LOCUS       DASSWR010000001         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_113052,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000001 DASSWR010000000
VERSION     DASSWR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113052"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..401)
                     /locus_tag="VFO47_00005"
     CDS             complement(132..401)
                     /locus_tag="VFO47_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787111.1"
                     /translation="MWRRRGGLLVVLAVVLDGDPALGSKDRWLRDIPATLRTDLPKLK
                     AVVLFDRLATPVPGDPCAWLVDSSPGARAGFAAAGSDPYLNQPHK"
     gene            complement(427..975)
                     /locus_tag="VFO47_00010"
     CDS             complement(427..975)
                     /locus_tag="VFO47_00010"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HET8787112.1"
                     /translation="MRRRPFLLGLLALLSSTLVAGAALPAGAGGPKPPTVPAPIAVPA
                     GHKVVLDVTGKGVQIYDCKPSATDPSVLAWTFREPAAVLYGHRGRPVGIHFRGPTFES
                     FDGSSVVGAADGSVPAPDPGSVPWLRLKAVSTQGDGVLAGVDYIQRLETRGGVAPTGA
                     CDPATDATVAVPYRARYVFYAG"
     gene            complement(1123..1383)
                     /locus_tag="VFO47_00015"
     CDS             complement(1123..1383)
                     /locus_tag="VFO47_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787113.1"
                     /translation="MADPSSKADIQLNRSLIVGGGVLVALGGLLGFAGMALLSSALIS
                     ATRQWVDHLDQPPSEIARRRWAQTKHAATVGAAAWRDGPPSS"
     gene            complement(1477..1746)
                     /locus_tag="VFO47_00020"
     CDS             complement(1477..1746)
                     /locus_tag="VFO47_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010062891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HET8787114.1"
                     /translation="MDKQFTAQLQTSPNQGGWTYLVWPGSVEFFGTRGLVKVRGTIDG
                     HPFRSSFMALGDGTHKLPVKADVRRAIGKEAGDTVTVRLEERIDD"
     gene            complement(1772..2134)
                     /locus_tag="VFO47_00025"
     CDS             complement(1772..2134)
                     /locus_tag="VFO47_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787115.1"
                     /translation="MGRTTRITGVGTVGLPVTDQDRALEFYVGELGFETRRDVPFGDG
                     RWIEVAPPGAATTIALVPAGIPAGIRLTTEDAGADHAGLRARGVDADPEVLRIPEAPP
                     MFAVRDPDGNSLILVEDA"
BASE COUNT          317 a          792 c          875 g          319 t
ORIGIN      
        1 gggctcgcga cgacgcgtcg ccgatctcac cacagggacg atcgggcggc ctccagaagg
       61 cccacggggg aaacccgtcc cgggacgcgg acacgggaag cggcgtcccg ggacggtacc
      121 agcagagtag gctatttgtg cggttggttc aggtagggat ccgacccggc ggcggcgaac
      181 ccggccctgg ccccgggcga gctgtcgacc agccaggcgc acgggtcacc ggggacgggg
      241 gtggccagcc ggtcgaacag gaccaccgcc ttgagcttgg gcaggtcggt ccggagggtc
      301 gccgggatgt cgcgcagcca ccggtccttg ctcccaaggg ccgggtcgcc gtccagcacg
      361 accgcgagca cgaccaggag cccgccgcgc cgtcgccaca tgcgcggcag tgtcccacgg
      421 ggagggtcag ccggcgtaga acacgtagcg ggcccggtag ggcacggcca cggtggcgtc
      481 ggtcgccggg tcacaggcgc cggtcggcgc caccccgccc cgggtctcca gccgctggat
      541 gtagtcgacc ccggcgagca ccccgtcacc ctgggtggag accgccttca gccgcagcca
      601 ggggaccgac ccgggatcgg gggcgggcac gctgccgtcg gccgccccga ccacgctgct
      661 gccgtcgaac gactcgaagg tgggcccacg gaagtggatg ccgaccgggc gtccccggtg
      721 gccgtagagc acggccgctg gctcccggaa cgtccaggcg agcacgctag ggtcggtggc
      781 gctgggcttg cagtcgtaga tctgcacgcc cttgccggtg acgtccagca cgaccttgtg
      841 gccggccggg acggcgatgg gcgcggggac cgtcggcggc ttcggcccgc cggcgcccgc
      901 cgggagcgcc gcgccggcga ccagggtcga gctgagcagg gcgagaagtc cgagcaggaa
      961 cgggcggcgt cgcatgacac gacctcctcg aggagcgttg cgggggagcg tcgcccggcc
     1021 ctgggcgaca gacccgcgga tcctgcgaca gtccgaccga aagcgcaaat cgccatgccc
     1081 ggcccgaact gggccgaacg cggcgcttct tcctgctggc ggctagctgc tcggggggcc
     1141 gtcgcgccag gcggcggcgc cgacggtggc ggcgtgcttg gtctgggccc agcggcgcct
     1201 ggcgatctcg ctcgggggct ggtccaggtg gtcgacccac tggcgggtcg ccgagatcag
     1261 cgcggagctg agcagggcca tgccggcgaa gccgagcagg ccgccaaggg cgaccagcac
     1321 gcctcctccg acgatcaggc tgcggttgag ctggatgtca gctttggagc tgggatctgc
     1381 cattgccttg cccccttgtc gtcgaccggc atcatgatgc ccagccgggt tgggccgggc
     1441 atcacacttc ctgggtgatt ctgcgcgggt atgggtctag tcgtcgatcc gctcctccag
     1501 ccggacggtc acggtgtcgc cggcctcctt gccgatggcc cggcggacgt cggccttcac
     1561 gggcagcttg tgggtgccgt cgcccagggc catgaaggag ctccggaacg ggtggccgtc
     1621 gatcgtgccc cggaccttga ccaggccccg ggtgccgaag aactcgaccg acccgggcca
     1681 caccaggtag gtccagccgc cctggttggg gctcgtctgc agctgcgcgg tgaattgctt
     1741 gtccatggtc gcccccacag gtcgtcgccg ctcaggcgtc ttcgacgagg ataaggctgt
     1801 tgccgtccgg gtcgcgcacc gcgaacatcg gcggcgcctc cgggatccgc agcacctccg
     1861 ggtcggcgtc gacgccgcgg gcccgcagcc cggcgtggtc ggcgccggcg tcctcggtgg
     1921 tgaggcggat gccggccggg atgccggccg ggacgagggc gatggtggtg gccgcccccg
     1981 gcggggccac ctcgatccag cgcccgtcgc cgaaggggac gtcgcggcgg gtctcgaagc
     2041 cgagctcgcc gacgtagaac tcgagcgccc gatcctggtc ggtgacgggg aggccgaccg
     2101 ttccgacgcc ggtgatccgg gtggtcctgc ccatttcctg ctcctctcgc gggatgtccg
     2161 tgctcgcggg gtcaccagat gatcgaagct ggcgagctag tctcgacatc cctgaggaag
     2221 cgcgttcgag atccgaggag gccccgtgaa gtacctgatc ctgatctaca gcaacccggc
     2281 gtcgcgggag atctgggagg ggt
//
LOCUS       DASSWR010000002         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_876007,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000002 DASSWR010000000
VERSION     DASSWR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876007"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1034)
                     /locus_tag="VFO47_00030"
     CDS             complement(15..1034)
                     /locus_tag="VFO47_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787116.1"
                     /translation="MSAPTAKLLEVEDLQVRFYTRRGVAHAVRDVSLSLDRGETLGLV
                     GESGSGKSVTAQAILGLIELPGKVTHGEIRWKGHSLLGPEGARLAGRIRGKELAVVFQ
                     DPMTSLNPLFPVKTQIGEVLRRHLGMSRKQAHERVVELLELVGVASPRRRADQYPHEL
                     SGGMRQRVMIAMALACEPSLLIADEPTTALDVTIQAQILELIAELQQRLQLAVLLIAH
                     DLGVVAGVCHRVAVMYGGKIVETGSAPELFRRPGHPYTAGLLGSTPRLDLVQHRLSGI
                     DGVPPELLDPPPGCPYEPRCPLAVEQCASEMPPLADHDGREVACWRAFATTDGAPAGA
                     RSLPG"
     gene            complement(1031..1966)
                     /locus_tag="VFO47_00035"
     CDS             complement(1031..1966)
                     /locus_tag="VFO47_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009572110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787117.1"
                     /translation="MFLRGRPYEVIRSVRGALGELWSDKAGLFGVGVLVLLVLTAVFA
                     PLLAPHDPAAQSLQNRLRPPFWYPEGSLENPLGADGLGRDILSRMIYGSRVSLIVGAS
                     VVLMAGTFGTLMGLIAGYRGGRTDSIIMRVVDTQVAFPGLLLVLIILAAIGPSMTTII
                     VVLALNGWMVYARITRGVVLSVKEMPYVEAAEIVGCPSKRVVLRHILPNLASPLLTLA
                     VLEFARIILAEAALSFLGVGIQPPQVSWGLMVAQGRDYLFNGWWIITWPGLAIALTVL
                     GINLFASWLRVTADPQEREKRFARSATEMRQGSVT"
     gene            complement(2046..>2339)
                     /locus_tag="VFO47_00040"
     CDS             complement(2046..>2339)
                     /locus_tag="VFO47_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8787118.1"
                     /translation="KGMPRARVVGFHALRNASIPALTLTGWELIRALAGYSVVVETVF
                     AWPGIGFLADQALNNQDLILLQAIVFVVAVMVVVINVVIDVLYRAVDPRIKLV"
BASE COUNT          365 a          831 c          841 g          302 t
ORIGIN      
        1 cccgcagcag cggctcagcc ggggagcgac cgggctcctg cgggcgctcc gtcagttgtg
       61 gcgaacgcac gccagcatgc cacctccctc ccatcgtggt cggccagcgg cggcatctcg
      121 gaggcgcact gctccaccgc cagcgggcac cgtggctcgt acgggcagcc gggcggcggg
      181 tccagcagct cgggcgggac cccatcgatg ccgctcagcc ggtgctgaac caggtccagg
      241 cggggggtgg agcccagcag cccggcggtg tagggatggc ccgggcggcg gaacagctcg
      301 ggcgccgacc cggtctcgac gatcttgccc ccgtacatga ccgcgacccg gtggcagacg
      361 ccggccacca ccccgaggtc gtgggcgatg agcagcacgg ccagctgcag ccgctgctgc
      421 agctcggcga tgagctccag gatctgggcc tggatggtga cgtccagcgc ggtggtcggc
      481 tcgtcggcga tgagcagcga gggctcgcac gccagggcca tggcgatcat gacccgctgg
      541 cgcatgccgc cggacagctc gtgcgggtac tggtcggccc gccggcgcgg cgaggccacc
      601 ccgaccagct ccagcagctc cactacccgc tcgtgggcct gcttgcggct catgcccagg
      661 tggcggcgga gcacctcgcc gatctgggtc ttgaccggga acagcgggtt cagcgaggtc
      721 atggggtcct ggaagaccac cgccagctcc ttgccgcgga tgcggccggc caggcgcgcg
      781 ccctcggggc cgagcagcga atggcccttc cagcggatct cgccgtgggt gaccttcccc
      841 gggagctcga tcagaccgag gatggcctgg gcggtgacgc tcttgcccga cccggactcg
      901 ccgaccaggc cgagcgtctc gccccggtcc agcgacaggc tgacgtcgcg gacggcatgg
      961 gccacgcccc gccgggtgta gaagcggacc tggaggtcct cgacctcgag cagcttggcg
     1021 gttggggcgc tcatgtgacc gacccctgac gcatctcggt cgcggagcgg gcgaagcgct
     1081 tctcgcgctc ctgggggtcg gcggtcaccc gcagccagct cgcgaacagg ttgatcccga
     1141 gcacggtgag ggcgatggcc agccccggcc aggtgatgat ccaccagccg ttgaacaggt
     1201 agtcgcggcc ctgggcgacc atcagccccc aggagacctg cgggggctgg atgccgacgc
     1261 cgaggaagga cagggccgcc tcggccagga tgatccgggc gaactcgagc acggccaggg
     1321 tgagcagcgg cgaggccagg ttgggcagga tgtgccgcag gaccacccgc ttggaggggc
     1381 agccgacgat ctcggccgcc tcgacgtacg gcatctcctt gacgctcagc accacccccc
     1441 gggtgatgcg ggcgtacacc atccagccgt tgagggccag gaccacgatg atggtggtca
     1501 tgctcggccc gatggcggcc aggatgatga gcaccagcag caggcccggg aaggcgacct
     1561 gggtgtcgac cacccgcatg atgatcgagt cggtgcgccc gccccggtag ccggcgatca
     1621 gccccatgag cgtgccgaag gtaccggcca tcagcaccac cgacgccccg acgatcagcg
     1681 acacccgcga gccgtagatc atccgcgaga ggatgtcgcg cccgagcccg tcggccccca
     1741 gcgggttctc caggctgccc tcggggtacc agaacggcgg ccggaggcgg ttctggagcg
     1801 actgcgccgc cgggtcgtgc ggcgccagca gcggcgcgaa caccgccgtc agcaccagca
     1861 ggaccagcac gccgacgccg aacaggcccg ccttgtcgct ccacagctcg cccagcgcgc
     1921 cccggacgga ccggatgacc tcgtagggcc gcccgcgcag gaacatctgg tgctgggcgg
     1981 cgtgctcggt ctcccgccgt cgccgcttgc cgagatcggg ttcgcgtccc ttgaccgttg
     2041 ccatgtcaca ccagcttgat ccgggggtcg accgcccggt agaggacgtc gatcaccacg
     2101 ttgatgacca ccaccatgac ggcgaccacg aagacgatcg cctggagcag gatcaggtcc
     2161 tggttgttga gggcctggtc ggccaggaag ccgatgccag gccaggcgaa gacggtctcc
     2221 accaccaccg agtagccggc cagggcgcgg atcagctccc agccggtcag ggtcagcgcc
     2281 gggatcgagg cgttgcgcag cgcatggaag ccgaccaccc gggcccgcgg catgccctt
//
LOCUS       DASSWR010000003         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_423928,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000003 DASSWR010000000
VERSION     DASSWR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423928"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFO47_00045"
     CDS             complement(<1..963)
                     /locus_tag="VFO47_00045"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET8787119.1"
                     /translation="MHTTAATARTGVRGRALRIGAAVLLAVATVSLLGVSATDAAGSR
                     GHLDVSRERFGTMPDGTVVHRYTLTNKRGMEVKLITYGGAIQSLRVPDRHGRKANVTL
                     GFSTLEEYLNPANPYFGCITGRYANRIDEGRFTLDGVTYQLAINNPPNHLHGGNVGFD
                     KRVWKAEVVRADKFVGVRFTYTSPDGEENYPGTLKTTVTYTLNNRNDLRMRYQATTDA
                     PTIVNLTNHAYWNLAGEGSGDINDHELLLKASHYTPVDPTLIPTGAIDPVAGTPMDFT
                     RSTRIGKRIRDYGFEQLQIGRGYDHNWVLDRRVSGLELAARLKDP"
     gene            complement(1000..2157)
                     /locus_tag="VFO47_00050"
     CDS             complement(1000..2157)
                     /locus_tag="VFO47_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8787120.1"
                     /translation="MVGDRGDLGIGMVGYAFMGRAHSQAWRTVGRFFDLPFTPRMAAI
                     CGRDASAVAAAADRLGWPATETDWKRLVERDDVDLVDVCTPGSSHAEISIAALEAGKH
                     VLCEKPLANTVDEARAMAEAASRAAASGVRAMVGFNFRRVPAVALARELIAAGRLGAI
                     RHVRGAYLASHVLDPEFPLLWRLQAEHAGSGALGDLGAHAIDLAQHLAGDRIAGVSAL
                     TETFVRERPLPGGGGARGPVTVDDAALFIARFAGGAVGSFEATRFATGHTEGLRVEVN
                     GELGSVIWELGALNELRLFDATEDPATQGFRRIQVTRAGHPYAGAWWPDGHTIGYEHT
                     FTHEVRDLLHDIADGRDPAPSFADGLQVQEVLDAVQRSAASSAGWTLVGRQ"
     gene            2308..>2773
                     /locus_tag="VFO47_00055"
     CDS             2308..>2773
                     /locus_tag="VFO47_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787121.1"
                     /translation="MRTGRSPARQDALRHHNLALVLQCIAASEPVSRARIAATTSLTK
                     TTVSSLVDDLVAARLVTELGPEARGEIGRPGSALALNRAGFVGVGLEINVDYIAVCVA
                     NLVGEVRYLRTRARDNRAQSPAKVLGRAVRMTRTAIEAAEAAGLTVAGLAVAV"
BASE COUNT          355 a          944 c         1054 g          420 t
ORIGIN      
        1 cgggtccttg agccgcgccg ccagctccag gccggagacg cgccggtcga gcacccagtt
       61 gtggtcgtag ccgcggccga tctgcagctg ctcgaacccg tagtcgcgga tgcgcttgcc
      121 gatccgggtc gaccgggtga agtccatcgg cgtgccggcc accgggtcga tggccccggt
      181 ggggatcagg gtcgggtcga ccggcgtgta gtggctggcc ttgagcagca gctcgtggtc
      241 gttgatgtcg cccgagccct cgccggccag gttccagtag gcgtggttgg tcaggttgac
      301 gatcgtcggg gcgtcggtgg tcgcctggta gcgcatccgt aggtcgttgc ggttgttcag
      361 ggtgtaggtg acggtggtct tgagggtgcc cgggtagttc tcctcgccgt cggggctggt
      421 gtaggtgaag cgcacgccga cgaacttgtc ggcgcggacg acctcggcct tccacacccg
      481 cttgtcgaag ccgacgttgc cgccgtgcag gtggttgggc gggttgttga tggccagctg
      541 gtaggtgacg ccgtcgaggg tgaagcggcc ctcgtcgatg cggttggcgt agcggccggt
      601 gatgcagccg aagtacgggt tggccgggtt gaggtactcc tcgagggtgc tgaagccaag
      661 ggtgacgttg gccttgcggc cgtggcggtc gggtacccgg agcgactgga tcgcgccgcc
      721 gtaggtgatc agcttcacct ccatgccgcg cttgttggtg agggtgtagc ggtggacgac
      781 ggtgccgtcg ggcatcgtcc cgaaccgctc ccgggagacg tcgaggtggc ctcgcgagcc
      841 ggcggcgtcc gttgcgctca cccccaggag gctgacggtg gcgacggcca gcaggacggc
      901 cgcgccgatg cgcaacgccc gtccccggac cccggtcctc gcggttgcgg cggtggtgtg
      961 catggaccct ccttatccgg tgcccatctc gggcatgagc tactggcgcc cgaccagcgt
     1021 ccagccggcg ctcgacgcgg cgctgcgctg caccgcgtcg agcacctcct gcacctggag
     1081 cccgtcggcg aacgacggcg ccgggtcgcg gccgtcggcg atgtcgtgga gcaggtcgcg
     1141 cacctcgtgg gtgaaggtgt gctcgtagcc gatcgtgtgc ccgtccggcc accaggcccc
     1201 ggcgtacggg tggccggccc gggtgacctg gatgcgccgg aacccctggg tggccgggtc
     1261 ctcggtggca tcgaacaggc gcagctcgtt gagggcgccc agctcccaga tgacgctgcc
     1321 gagctcgccg ttgacctcga cccggagccc ctcggtgtgg ccggtggcga agcgggtggc
     1381 ctcgaagctc ccgaccgccc cgccggcgaa gcgggcgatg aacagcgcgg cgtcgtccac
     1441 ggtgaccggc ccgcgggccc cgccgccgcc gggcagcggc cgctcgcgca cgaacgtctc
     1501 ggtcagggcc gagaccccgg cgatgcggtc gccggccagg tgctgggcca ggtcgatcgc
     1561 gtgggcgccg aggtcgccga gggcgcccga ccccgcgtgc tcggcctgga gccgccagag
     1621 cagggggaac tcggggtcga ggacgtggct ggccaggtag gcgccgcgga cgtggcggat
     1681 ggcgccgagg cggccggcgg cgatcagctc acgggccagg gcgacggccg gcacccggcg
     1741 gaagttgaag ccgaccatcg ccctgacgcc cgaggcggcg gccctggacg cggcctcggc
     1801 catggcccgc gcctcgtcga cggtgttggc cagcggcttc tcgcacagca cgtgcttgcc
     1861 ggcctccagg gccgcgatcg agatctcggc gtggctggac ccgggggtgc agacgtcgac
     1921 caggtcgacg tcgtcccgct cgaccagccg cttccagtcg gtctcggtgg ccggccagcc
     1981 gagccggtcg gcggcggccg cgaccgcgct ggcgtcccgg ccgcagatgg cggccatcct
     2041 gggcgtgaag gggaggtcga agaagcgccc gacggtccgc caggcctggg agtgggcccg
     2101 gcccatgaag gcgtagccga ccatcccgat cccgaggtca ccccggtcgc ccaccatacg
     2161 aacccccgac ttccgcttcg ggaacttcgt agaacctgga gtttgtccgt tcagtggcca
     2221 aactaagctg ggcctccttt ccctgtcaat gcctagtcgg tagagtatgt ccagggactg
     2281 gacaaacatc ctgggggagg tcttgtcgtg aggacggggc ggtcgccggc caggcaggat
     2341 gcgctccgtc accacaacct cgccctggtg ctccagtgca tcgcggccag cgagccggtg
     2401 tcccgggccc gcatcgccgc caccaccagc ctgaccaaga cgaccgtctc cagcctggtc
     2461 gacgacctgg tcgccgcccg cctggtcacc gagctgggcc cggaggccag gggcgagatc
     2521 ggccggccgg ggagcgccct ggccctcaac cgggccggct tcgtcggcgt cggcctcgag
     2581 atcaacgtcg actacatcgc cgtgtgcgtg gccaacctgg tcggggaggt ccgatatctc
     2641 agaacccggg cgcgcgacaa ccgggcccag tcccccgcca aggttctggg gcgggccgtg
     2701 cgcatgaccc gcacggccat cgaggccgcc gaggcggccg ggctgaccgt ggccgggctg
     2761 gccgtggccg tgc
//
LOCUS       DASSWR010000004         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1186887, whole genome shotgun sequence.
ACCESSION   DASSWR010000004 DASSWR010000000
VERSION     DASSWR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186887"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="VFO47_00060"
     CDS             <1..2206
                     /locus_tag="VFO47_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8787122.1"
                     /translation="FPTSLSPEALARFERREQVAATAAMQAFLGPRSVAVVGASRRRG
                     TVSGELFHNLLAAGFNGPVYPVNPKAAVVQSVLAYRSVLDVPGPVDLAVLALPAPYVV
                     EAAQECAAKGVRAVVVISAGFAETGPEGVERQRELLAVCRAAGMRLIGPNCLGIVNTD
                     AEVCLDATFGPIVPLPGRVGFLSQSGALGLAIIDYANALGLGLSSFVSVGNKADISAN
                     DLLSYWEQDDRTNLVLLYLESFGNPRKFARIARRVARTKPVLAVKSGRSAAGARATSS
                     HTGALLAASDVTVDALFDQAGVIRTDSLAELFDVASLLVNQPVPAGRRVGIVTNAGGP
                     GIMCADACEAGGLEVVQLSPRLQASLADGLPAEAALSNPVDMLASAPAEHYRRTVELV
                     AASGEVDAVIAIFIPPLRANPAAVARAVRDAAATAGAVPVLSVVMSAHELPAEANGGA
                     PRLPRYRFPEDAARALVRAVEYATWRQRPEGHVPALPDARHDEAAALLAATLADGSGP
                     RWLAPEEVARLLACYGVPVAEWRLAGSPEEAAAAAVELGGPVALKAVAPRLIHKTEAH
                     AVRLHLTGADQVRTAAGEMAVAVAAEGYTVNGFLVQRMVGEGVELLVGVVHDASFGPV
                     IACGAGGTAVELLKDVAVRITPLTEVDAAEMVRSLATYPLLDGYRGAPRADVAALEDL
                     LLRVSALVEAHPEVAELDLNPVRALADGVMVVDARIRVEPAAPSLPLGARRR"
     gene            complement(2233..>2521)
                     /locus_tag="VFO47_00065"
     CDS             complement(2233..>2521)
                     /locus_tag="VFO47_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HET8787123.1"
                     /translation="AKRGPTGLIGNNKPDSAATVEAMLADLPLLRRFRDRHRDPSAIE
                     ALLRERGVDHVTYPDWQVLDAHEVAAGRAEGRPRKKVTTVPEMMAIIHQGR"
BASE COUNT          288 a          938 c          925 g          370 t
ORIGIN      
        1 gttcccgacc tccctgtccc cggaggcgct ggcgcggttc gagcggcgcg agcaggtggc
       61 cgccacggcc gccatgcagg ccttcctggg cccgcggtcg gtggccgtgg tcggcgcctc
      121 ccggcgccgt ggcaccgtct ccggtgagct gttccacaac ctgctggcag ccgggttcaa
      181 cggcccggtc tacccggtca accccaaggc cgcggtggtc cagtcggtcc tggcctacag
      241 gtcggtcctg gacgtgcccg gcccggtcga cctggccgtg ctggccctgc cggcgcccta
      301 tgtggtcgag gcagctcagg agtgcgcggc caagggggtg cgggcggtcg tggtcatctc
      361 ggccggcttc gccgagaccg ggccggaggg cgtcgagcgc cagcgggagc tgctggccgt
      421 ctgccgcgcc gccgggatgc gcctgatcgg tcccaactgc ctggggattg tgaacaccga
      481 tgccgaggtg tgcctggacg ccaccttcgg cccgatcgtc ccgctgcccg ggcgggtggg
      541 gttcctgtcc cagagcggcg ccctggggct ggccatcatc gactacgcca acgcgctcgg
      601 gctggggctg tcgtcgttcg tgtcggtcgg caacaaggcc gacatctccg ccaacgacct
      661 cctctcctac tgggagcagg acgaccggac caacctggtg ctgctgtacc tggagtcgtt
      721 cggcaacccg cgcaagttcg cccgcatcgc ccgccgggtg gccaggacca agcccgtgct
      781 cgcggtcaag agcggccgct cggccgccgg agccagggcc acctcctccc ataccggggc
      841 cctgctggcc gcctccgacg ttacggtgga cgccctgttc gaccaggcgg gcgtgatccg
      901 caccgatagt ctcgccgagc tgttcgacgt ggcctccctg ctcgtcaacc agccggtccc
      961 ggccggtcgg cgcgtgggga tcgtcaccaa tgccggcggg ccggggatca tgtgtgccga
     1021 cgcctgcgag gccggcggcc tggaggtcgt ccagctctcc ccgcggctcc aggccagcct
     1081 ggccgacggc ctgcccgccg aggcggccct gtccaacccg gtcgacatgc tggcctcggc
     1141 cccagccgaa cactaccgcc gcacggtcga gctggtggcc gcctccgggg aggtcgatgc
     1201 cgtcatcgcc atcttcatcc cgcccctgcg cgccaacccg gcggcggtcg cccgagcggt
     1261 ccgcgacgcg gccgcgacgg ccggagcggt cccggtgctg agcgtggtga tgtcggcaca
     1321 cgagctgccg gccgaagcga acgggggcgc gccgcggctt ccccgctacc ggttccccga
     1381 ggacgcagcc cgcgcgctcg tccgggcggt ggagtacgcg acctggcgcc agcgcccgga
     1441 gggccatgtg cccgcgctgc cagacgcccg ccacgacgag gccgccgccc tgctggctgc
     1501 caccctggcc gacgggtcag gcccccgctg gctggcgccg gaggaggtag cgaggctgct
     1561 cgcctgctac ggcgtgccgg tcgccgagtg gcgcctggcc ggctccccgg aggaggccgc
     1621 cgcggccgcg gtcgagctcg gtgggccggt cgccctcaag gcggtcgcgc cgcggctgat
     1681 ccacaagacc gaggcccacg ccgtccggct ccacctcacc ggcgccgacc aggtccggac
     1741 cgccgccggc gagatggccg tggccgtcgc cgccgagggc tacacggtga acgggttcct
     1801 ggttcagcgc atggtcggcg agggtgtgga gctgctggtc ggcgtcgtcc acgacgcctc
     1861 cttcggtccc gtgatcgcct gtggggcggg cggcacggcg gtggagctcc tcaaggacgt
     1921 ggccgtgcgc atcaccccgc tgaccgaggt agatgcggcc gagatggtcc gctcgctggc
     1981 cacctacccg ctgctggacg gctaccgggg cgccccaaga gccgacgtgg ccgccctcga
     2041 ggacctgctg ctgcgggtca gcgccctggt cgaggcccac ccggaggtcg ccgagcttga
     2101 tctcaatccg gtgcgggcgc tggccgacgg cgtcatggtg gtcgacgcca ggatccgggt
     2161 cgagccggcc gccccatcgc tcccgctggg cgcccgccgc cgatagccac gcccgctggc
     2221 gggcgccggc tctcatcgtc cctggtggat gatggccatc atctcgggaa cggtcgtgac
     2281 ctttttccgc gggcggcctt cggctcgacc ggcggcgacc tcgtgggcat ccaggacctg
     2341 ccagtccgga taggtgacgt ggtcgacgcc tcgctcgcgc aggagcgcct cgatcgccga
     2401 cgggtcgcga tgcctgtcgc ggaaccgtcg taggaggggc aggtcggcca gcatggcctc
     2461 gacggtggcg gcggaatcgg gcttgttgtt gccgatcaga cccgtcgggc cccgcttggc
     2521 c
//
LOCUS       DASSWR010000005         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_847912,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000005 DASSWR010000000
VERSION     DASSWR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847912"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFO47_00070"
     CDS             <1..340
                     /locus_tag="VFO47_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8787124.1"
                     /translation="KVAQLSDAVLSYASLLSWEQHGLGEQFLTLARGFWRTRGFGDFW
                     GHMLVAEGAADVAIEPEVELWDLAAPQVIVEEAGGRFTDLRGTPTPAGRSVVSSNGLL
                     HDRVVATLRP"
     gene            424..1221
                     /locus_tag="VFO47_00075"
     CDS             424..1221
                     /locus_tag="VFO47_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8787125.1"
                     /translation="MALVTGVSRRIGIGMAVARRLAALGTDLFVTSWTAHDREQPWGA
                     DPGGMDAVLAELRAGAAPRQAPGAGDPAGRRVEHLEADFLDPEAPGRVMAAAVAAYGR
                     VDILVCNHARSSEGDLARLTAAELDTTLAVNTRAVLLLVQAFAAQHDGRPGGRVVLFT
                     SGQQLGPMPDELAYATSKGALVAITPTLADTLADRGITVNTVNPGPTDTGYADEAVRE
                     RVATRFPAGRWGTPDDVARLVAWLCTDEAAWVTGQVINSEGGFRRYG"
     gene            1279..2043
                     /locus_tag="VFO47_00080"
     CDS             1279..2043
                     /locus_tag="VFO47_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787126.1"
                     /translation="MHGPAGVLTVVLYETALGGLALLWLAPLWGVVRPGFFKLAGATV
                     LACATLAWLAGRGELAAAGATGRMAALWLGVFAAAVLVWQVLLYARVAAARWAGMAAV
                     LPGLVAGWPLAQLSGRPALGALALGTGALFLGATLDGLLLGHWYLVDRRLSNRPIAAL
                     ATWLVAGVAAALVSAALGGARGSAVDQSLSPLLAFPNLTVYLAVGLVLLVGLMASFIR
                     VLVKGNSIQAATGMFYLAVIMALAAEFAAKVYFFPA"
BASE COUNT          230 a          815 c          811 g          293 t
ORIGIN      
        1 caaggtcgcg cagctgtccg acgccgtcct gagctacgcc agcctcctgt cgtgggagca
       61 gcacgggctc ggcgagcagt tcctcaccct cgcccgcggc ttctggcgga ccagggggtt
      121 cggggacttc tgggggcaca tgctggtcgc cgagggcgcg gccgacgtcg ccatcgagcc
      181 ggaggtcgag ctgtgggacc tggcggcgcc tcaggtgatc gtcgaggagg cgggcggccg
      241 cttcaccgac ctccgcggca ccccgacccc ggccggccgc agcgtggtct ccagcaacgg
      301 cctcctgcac gaccgggtgg tcgcgaccct gcggccgtga cctccgagga ccccgcccca
      361 ccgtgggcct tgaccggcga gggccccgac cggccggccc ccagatggcc cctcgatggg
      421 cgggtggcgc tggtgaccgg ggtcagccgg cggatcggca tcgggatggc ggtggccagg
      481 cggctggcgg cgctcgggac cgacctgttc gtcacctcgt ggaccgccca cgaccgggag
      541 cagccctggg gggccgaccc gggcggcatg gacgcggtcc tggccgagct gcgggctggc
      601 gccgcccccc ggcaggcacc cggagccggc gacccggcgg ggaggcgggt cgagcacctg
      661 gaggccgact tcctcgaccc ggaggcgccc ggccgggtga tggcggccgc cgtcgcggcg
      721 tacgggcggg tggacatcct cgtctgcaac cacgcccgca gcagcgaggg ggacctggcg
      781 cggctcaccg cggccgagct ggacaccacc ctcgccgtca acacgcgggc cgtgctcctg
      841 ctcgtccagg ccttcgccgc ccagcacgac gggcggccgg gtggccgggt ggtgctgttc
      901 acctccggcc agcagctcgg gcccatgccg gacgagctcg cctacgcgac ctccaagggc
      961 gcgctggtcg ccatcacccc caccctggcc gacacgctgg ccgaccgggg gatcaccgtg
     1021 aacaccgtca accccggccc gaccgacacc ggctacgccg acgaggcggt ccgcgagcgg
     1081 gtggcgacga ggttcccggc cggccgctgg ggcactcccg acgacgtcgc ccggctggtc
     1141 gcctggctct gcaccgacga ggcggcctgg gtcacggggc aggtcatcaa ctccgaggga
     1201 gggttccgcc gctacgggtg acgcggaggc gctaccctgc cacggcacaa ccacacggcg
     1261 gagaggagga gcggcaccat gcacggtccc gccggagtgc tcacggtggt cctgtacgag
     1321 accgccctcg gcggcctggc cctgctctgg ctggcgccgc tgtggggcgt ggtccggccc
     1381 ggcttcttca agctggccgg ggcgaccgtg ctggcctgcg cgacgctggc ctggctggcc
     1441 ggccggggcg agctggccgc cgccggagcc accggccgca tggccgccct ctggctgggg
     1501 gtgttcgcgg ccgccgtgct cgtctggcag gtgctgctct acgcccgcgt ggccgcggcc
     1561 cgctgggccg gcatggccgc ggtcctgccc gggctggtcg ccggctggcc gctggcccag
     1621 ctgagcggac gccccgccct tggggcgctg gccctgggaa ccggggccct gttcctgggc
     1681 gcgaccttgg acgggctgct gctcggtcac tggtacctgg tcgaccggcg gctgtccaac
     1741 cggcccatcg ccgccctcgc cacctggctc gtcgccgggg tggcggccgc cctcgtctcg
     1801 gccgccctcg gcggggccag gggcagcgcc gtcgaccagt cgctctcgcc actgctggcc
     1861 ttccccaacc tcaccgtgta cctggccgtc ggcctggtgc tgctggtcgg gctgatggcc
     1921 tccttcatcc gggtgctggt caagggcaac tccatccagg ccgccaccgg catgttctac
     1981 ctggccgtga tcatggccct ggcggccgag ttcgcggcca aggtgtactt cttcccggcc
     2041 tgagccgggg gcgacgaaga tttgcccggg ctggtcgccg gctggccgct ggcccagctg
     2101 agcggacgcc ccgcccttgg ggcgctggcc ctgggaaccg gggccctgt
//
LOCUS       DASSWR010000006         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1045783, whole genome shotgun sequence.
ACCESSION   DASSWR010000006 DASSWR010000000
VERSION     DASSWR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045783"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..605
                     /locus_tag="VFO47_00085"
     CDS             168..605
                     /locus_tag="VFO47_00085"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8787127.1"
                     /translation="MAASDSTAGFGPLEGSKTALLTTYRDGGEAVGTPLSIALTGGKV
                     YFVTAGDSGKARRLATTARVTLAPCTIGGKVTGETVHGRARRLDPSERKTASKGLLKP
                     TGPLFWSLVMYRLFEGKKMELFEVVPDDGPAPSGGQEPVTSRG"
     gene            complement(589..1224)
                     /locus_tag="VFO47_00090"
     CDS             complement(589..1224)
                     /locus_tag="VFO47_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET8787128.1"
                     /translation="MSARDEVLARVRAAVAGGAEPPPVARTYRTAGHPPELAGEELLE
                     RLVDRLVDYRALVRRSTTAMVATTVVAALTERGVRRVAVPDGILPAGLDRLEPGIELV
                     GDDPPLTPAQLDGLDGVLTGCAVAIAETGTIVLDGGAGQGRRVLSLVPDYHLVVVTAA
                     QVVAGVPEALARLDPARPQTWISGPSATSDIELDRVEGVHGPRTLEVILCS"
     gene            complement(1221..>2099)
                     /locus_tag="VFO47_00095"
     CDS             complement(1221..>2099)
                     /locus_tag="VFO47_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET8787129.1"
                     /translation="AEAARLHLRRKFLEAEVAVSGANFAVAETGTLAVVESEGNGRMC
                     LTLPRVLITVMGIEKLVPAWRDLEVFLQLLPRSSTGERMNPYTSMWTGVHPGDGPQEF
                     HLVLLDNGRTATLADPQGRAALRCIRCSACLNVCPVYERTGGHAYGSVYPGPIGAVLS
                     PQLTGVADNASLPYASTLCGACYDVCPVAIDIPQMLVHLRSRVVEAKREASKVPSAEQ
                     AVMASAAWIMDSPARWAGALRAARAGRLLGRRRRRISSLPPPLSAWTGTRDAPLPPTQ
                     PFRDWWAKRQRQGGRS"
BASE COUNT          273 a          770 c          801 g          255 t
ORIGIN      
        1 agccgggcct ccggctccgc ctcgcgcatg tccgccattc tcccctgtcc ctcgcgatcc
       61 acatcgccat cgtgggcgga gatccttggc ggcggcttgg gccttcctgg gaggttgctg
      121 ggagcataat ctgcttcgac gtcgacaccg cctgaccggg ggtgcccatg gccgccagcg
      181 acagcaccgc gggcttcggc ccgctcgagg gctccaagac cgccctgctg accacctacc
      241 gggacggcgg ggaggcggtg ggcacgccgc tcagcatcgc cctgaccggg ggcaaggtct
      301 acttcgtcac cgccggcgac agcggcaagg cgaggcggct ggccaccacg gccagggtca
      361 ccctggcccc gtgcaccatc ggcggcaagg tcaccggcga gaccgtccac ggccgggccc
      421 ggcgcctcga cccgtccgag cgcaagacgg cctccaaggg cctgctgaag ccgaccggcc
      481 cgctgttctg gagcctggtc atgtaccggc tgttcgaggg caagaagatg gagctgttcg
      541 aggtcgtccc cgacgacggc cccgcgccct cgggcggcca ggagccggtc acgagcagag
      601 gatgacctcc agggtccgag ggccatggac gccctcgacc cggtcgagct cgatgtcgct
      661 ggtcgcgctg gggccgctga tccaggtctg ggggcgggcc gggtcgagcc gggccagcgc
      721 ctcggggacg ccggccacca cctgggcggc ggtcaccacc accaggtggt agtcggggac
      781 caggctgagg acgcggcggc cctggccggc accgccgtcg agcacgatcg tgcccgtctc
      841 ggcgatggcc acggcgcagc cggtgagcac gccgtcgagg ccgtcgagct gggccggggt
      901 gaggggcggg tcgtcgccga ccagctcgat gccgggctcg aggcggtcga gcccggcggg
      961 gaggatgccg tcggggaccg ccacccgccg caccccgcgc tcggtcaggg cggccaccac
     1021 cgtggtcgcg accatggcgg tggtgctgcg cctgaccagg gcccggtagt cgaccaggcg
     1081 gtcgaccagc cgctccagca gctcctcgcc ggccagctcg ggcgggtggc cggcggtccg
     1141 gtaggtccgg gccaccgggg gtggctcggc cccgccggcc acggcggccc ggacccgggc
     1201 caggacctcg tccctcgcgc tcacgacctg ccgccctgtc gctggcgctt ggcccaccag
     1261 tcgcggaagg gctgggtcgg gggcaggggg gcgtccctgg tgccggtcca ggccgacagg
     1321 ggcggcggca gggagctgat ccggcgccgc cgccggccca gcagccggcc cgccctggcc
     1381 gcccgcagcg cgcccgccca acgggccggg ctgtccatga tccaggccgc cgaggccatc
     1441 accgcctgct cggccgacgg caccttggac gcctcgcgct tggcctccac cacccgcgag
     1501 cggaggtgga ccagcatctg ggggatgtcg atggccaccg ggcagacgtc gtagcaggcc
     1561 ccgcagaggg tcgaggcgta cggcagcgag gcgttgtcgg cgaccccggt cagctggggc
     1621 gagagcacgg ccccgatcgg gcccgggtag accgacccgt aggcgtggcc gcccgtgcgc
     1681 tcgtacaccg ggcagacgtt caggcaggcc gagcagcgga tgcagcgcag ggcggcccgg
     1741 ccctgcgggt cggccagggt ggcggtgcgg ccgttgtcca gcaggacgag atggaactcc
     1801 tgcgggccgt cgcccgggtg caccccggtc cacatgctgg tgtaggggtt catccgctcg
     1861 ccggtggagg agcggggcag gagctggagg aacacctcca ggtcgcgcca ggccgggacc
     1921 agcttctcga tccccatgac ggtgatcagc acccggggca gggtcaggca catgcggccg
     1981 ttgccctcgg actcgaccac ggccagggtg ccggtctcgg ccacggcgaa gttggccccg
     2041 gacacggcca cctcggcctc caggaacttg cgccgcagat gcagccgggc cgcctcggc
//
LOCUS       DASSWR010000007         4126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_819821,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000007 DASSWR010000000
VERSION     DASSWR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819821"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFO47_00100"
     CDS             complement(<1..294)
                     /locus_tag="VFO47_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8787130.1"
                     /translation="MLSARDVEEALKERLLRGAYPRGGALPSVRALAAELGTSPSTVG
                     RAVQELARQGWVTATSRRGAVVRRDLPAGGTGVRDAEAAIRRLAVRWRLVGGDR"
     gene            complement(294..1043)
                     /locus_tag="VFO47_00105"
     CDS             complement(294..1043)
                     /locus_tag="VFO47_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET8787131.1"
                     /translation="MAVWDDVLPDTDRKVFEAAGWGRPSGLGRRPVLLVIDANYNFVG
                     DRPEPILESIKRWRYSCGERGWQGVAAIGRVLEPARALRLPVLYTTNPRRPDGFDLGV
                     WNLKNWRAGDDTDVQGHLGNEIVAEVAPRPGDLLVEKKKPSAFFGTPLVSYLVALRAD
                     TLLLCGTTTSGCVRASAVDAISYNFRVGIPEPCVWDRGELTHKVNLFDLQQKYCDVLE
                     LEEVLGYLDGLEPGLFDAELPAVGDAGSVAS"
     gene            complement(1063..2607)
                     /locus_tag="VFO47_00110"
     CDS             complement(1063..2607)
                     /locus_tag="VFO47_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8787132.1"
                     /translation="MPAREVDLKLVGGRVVTGDGVLQAGVAIDGGAVVAVARDDLLPP
                     ARRTIDVRGNHILPGIVDSEAHPGCYVPFEDDLAPESRAAACAGITTWGIQAPSTRLG
                     TRPFKEFVQADDVVPFSQAFATARATIERVSSIDCFLTFMMETDEQAEEIPLYCEEYG
                     VTSYKLYMQARRIPAADHNWPSRRAGLGVGIDDGTLYLVMEQVARYGDPAICSIHPEN
                     WEIARVFERRARELGHDDLAAWTDRSPHMLEAQHVRAYAYVAEQLGARMYIQHATTPE
                     TFSEIREARRRGVELYAQTGPAWLGFSPFDGWRINVPLRYRDTQEALWQALARGDVDV
                     VGSDHVVAWPPADRQAMYRQSIWDCRTGFSRVETFLPVMLTHGVHEGRITLERLVQVC
                     CENPARIYGLFPRKGTVAVGSDADLVVVDLSREVTVGPEHLNTRAGWSIMEGKTYHGW
                     PVLTLLGGRVVAEWADDAPGPRPVGEPSGRYLPRAVRPDVPKEPKDRLVRTGPDATLA
                     GIRPGV"
     gene            2669..3241
                     /locus_tag="VFO47_00115"
     CDS             2669..3241
                     /locus_tag="VFO47_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HET8787133.1"
                     /translation="MPLDIRKLVVHLEEVRAEGGRGDTGGPLRKVAALAVVPNPYAGR
                     PWSGDLDELVEPSGALAAELAAAALAVLGGPVESYGKAALDCEQEHANACITGVFGDA
                     LREAVGGGRAWLPSVTKRVPAGAAVDIPVCYRDAIWVRSHYDAVTVAVPDAPLAGELV
                     VGLALTNRGRLNARLGGLAKEEVVGQDGLR"
     gene            3238..>4126
                     /locus_tag="VFO47_00120"
     CDS             3238..>4126
                     /locus_tag="VFO47_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET8787134.1"
                     /translation="MTWDGTFDPGAFGPSGGSLELRNLRGVVTGRLGEAPVAAATLRA
                     EAGRVVSVGADPAEADVVVDAAGAVAAPGLVDTHCHVVFGDYTPRQHAVGFLESYLHG
                     GVTQVMSASEVHLPGRPRDRAGVKALAVAAQRSWSGFRPGGVKVRGGSLICEPVLREP
                     DFAELAAEGVRYMKVGFGAFGDPFEAVPLVGWARAAGFVVMSHSGGASIPGSSPITVD
                     HLLAMDPTIAGHANGGTTSLPDADLERLVDGSGMALQVVQAGNLRSGLLLLGLARDRG
                     RLDRVILGTDTPSGTGVMPL"
BASE COUNT          502 a         1506 c         1568 g          550 t
ORIGIN      
        1 ccggtccccg ccgaccagcc gccagcggac ggccaggcgg cggatggccg cctcggcgtc
       61 gcggaccccg gtcccgccgg ccggcaggtc gcgccggacc accgccccgc gccggctggt
      121 cgccgtcacc cagccctggc gggccagctc ctgcacggcc cggccgaccg tgctcgggct
      181 ggtcccgagc tcggcggcca gcgcccgcac cgacggcagg gccccgccgc gggggtaggc
      241 gccccgcagc agccgctcct tcagcgcctc ctcgacgtcc cgcgccgaca gcatcacgag
      301 gcgacgctcc cggcgtcgcc cacggccggg agctcggcgt cgaacagccc cggctccagg
      361 ccgtcgaggt agccgaggac ctcctccagc tcgaggacgt cgcagtactt ctgctggagg
      421 tcgaacaggt tgaccttgtg ggtcagctcg ccccggtccc agacgcacgg ctcggggatg
      481 ccgacccgga agttgtagga gatcgcgtcc accgccgagg cccgcacgca gcccgaggtg
      541 gtggtcccgc acagcagcag ggtgtcggcc cgcagggcga ccaggtagct gaccagcggg
      601 gtgccgaaga acgccgacgg cttcttcttc tcgaccagca ggtcgcccgg ccggggcgcg
      661 acctcggcca cgatctcgtt gccgaggtgg ccctggacgt cggtgtcgtc gccggcgcgc
      721 cagttcttga ggttccagac cccgaggtcg aagccgtcgg gtcggcgcgg gttggtggtg
      781 tagaggaccg ggaggcgcag cgcccgggcc ggctccagga cccggccgat ggcggccacg
      841 ccctgccagc cgcgctcccc gcaggagtag cgccagcgct tgatggactc gaggatcggc
      901 tcgggccggt cgccgacgaa gttgtagttg gcgtcgatca ccagcagcac gggccgtcgg
      961 ccgagccccg acggccggcc ccatccggcg gcctcgaaca ccttgcggtc ggtgtctgga
     1021 aggacgtcgt cccagaccgc catgtgcggt tgcctccttc tgtcagacac cgggccggat
     1081 gccggccagg gtggcgtcgg gcccggtgcg gaccagccgg tccttgggtt ccttggggac
     1141 gtcgggccgg acggcccggg gcaggtagcg gccgctgggc tcgcccaccg ggcgcggccc
     1201 gggcgcgtcg tcggcccatt cggcgacgac ccggccgccc agcagggtca gcaccggcca
     1261 gccgtggtag gtcttgccct ccatgatcga ccagccggcc ctggtgttca ggtgctcggg
     1321 gcccacggtg acctcgcggg acaggtcgac cacgaccagg tcggcgtcgg acccgacggc
     1381 caccgtcccc ttgcggggga acaggccgta gatccgggcc gggttctcgc agcacacctg
     1441 gaccagccgc tccagggtga tgcggccctc gtggacgccg tgggtcagca tcaccggcag
     1501 gaaggtctcg acccgggaga acccggtccg gcagtcccag atgctctggc ggtacatggc
     1561 ctggcggtcc gccgggggcc aggcgaccac gtggtcggag ccgaccacgt cgacgtcgcc
     1621 ccgggccagg gcctgccaga gcgcctcctg ggtgtcgcgg tagcgcaggg gcacgttgat
     1681 gcgccagccg tcgaacgggg agaagccgag ccaggccggc ccggtctggg cgtacagctc
     1741 gaccccgcgc cggcgggcct cgcggatctc ggagaacgtc tcgggggtgg tggcgtgctg
     1801 gatgtacatg cgcgccccga gctgctcggc cacataggcg taggcccgga cgtgctgggc
     1861 ctccagcatg tggggcgagc ggtcggtcca ggcggccagg tcgtcgtggc cgagctcgcg
     1921 cgcgcggcgc tcgaacaccc gggcgatctc ccagttctcc gggtggatgg agcagatggc
     1981 cgggtcgccg tagcgggcca cctgctccat gaccaggtac agggtgccgt cgtcgatccc
     2041 gaccccgagc ccggcccgcc ggctcggcca gttgtggtcg gcggccggga tgcggcgggc
     2101 ctgcatgtag agcttgtacg aggtgacccc gtactcctcg cagtacaggg ggatctcctc
     2161 ggcctgctcg tcggtctcca tcatgaaggt caggaagcag tcgatcgagg acacccgctc
     2221 gatcgtcgcc cgcgccgtcg cgaacgcctg ggagaacggg accacgtcgt cggcctgcac
     2281 gaactccttg aaggggcggg tgcccaggcg ggtgctgggg gcctggatgc cccaggtggt
     2341 gatgccggcg caggccgcgg cccggctctc gggggccagg tcgtcctcga aggggacata
     2401 gcagccgggg tgggcctcgg agtcgacgat cccgggcagg atatggttgc cccgcacgtc
     2461 aatcgttcgc cgggccgggg ggagcaggtc gtcgcgggcc acggcgacga cggcaccccc
     2521 gtcgatcgcg acccccgcct ggagcactcc gtcgccggtc accacccggc cgccgacgag
     2581 cttgaggtcg acctcccgtg ccggcatggg ctcccctccc gggtctgtct cttgtctcgg
     2641 acagcggcta gaggatagcg tatgcggcat gccgctggac atccgcaagc tggtcgtcca
     2701 cctcgaggag gtcagggccg agggtggccg cggcgacacc ggcgggcccc tgcgcaaggt
     2761 cgccgccctg gccgtggtcc ccaatcccta tgccggccgg ccctggagcg gcgacctgga
     2821 cgagctggtg gagccgtcgg gggcgctggc cgccgagctg gccgccgccg ccctggccgt
     2881 gctgggcggg ccggtggaga gctacggcaa ggccgccctg gactgcgagc aggagcatgc
     2941 caacgcctgc atcaccgggg tgttcggcga cgccctgcgg gaggccgtcg gcggcggcag
     3001 ggcgtggctg ccgtcggtca ccaagcgcgt cccggccggg gcggcggtgg acatcccggt
     3061 ctgctaccgg gacgccatct gggtgcggtc ccactacgac gccgtgaccg tggccgtgcc
     3121 ggacgcgccc ctggcgggcg agctggtggt cggcctcgcc ctcaccaacc ggggccggct
     3181 caacgcccgc ctgggcggcc tggccaagga ggaggtcgtc ggccaggacg gcctgcggtg
     3241 acctgggacg gcaccttcga cccgggcgcc ttcgggccgt ccggcggctc gctggagctg
     3301 cgcaacctgc gcggagtggt gaccggccgg ctcggggagg cgccggtggc ggcggcgacg
     3361 ctgcgggccg aggcgggccg ggtggtgtcg gtcggggccg acccggccga ggccgacgtg
     3421 gtcgtcgacg ccgccggggc ggtggccgcc cccggcctgg tcgacaccca ctgccacgtc
     3481 gtgttcggcg actacacccc gcgccagcac gccgtcggat tcctggagag ctacctgcac
     3541 ggcggggtca cccaggtgat gtcggcctcc gaggtccacc tgcccggccg gccccgcgac
     3601 cgggccgggg tcaaggccct ggccgtggcc gcccagcggt cctggagcgg gttccggccc
     3661 ggcggggtca aggtccgggg cgggtcgctg atctgcgagc cggtgctgcg cgagcccgac
     3721 ttcgccgagc tcgccgccga gggcgtgcgc tacatgaagg tcggcttcgg ggccttcggc
     3781 gacccgttcg aggccgtgcc gctggtcggc tgggcccggg cggccgggtt cgtggtcatg
     3841 tcccactccg ggggcgcctc catccccggg tcgagcccga tcaccgtcga ccacctgctg
     3901 gccatggacc cgaccatcgc cgggcacgcc aacggcggca cgaccagcct gcccgacgcc
     3961 gaccttgagc ggctggtcga cggctccggc atggccctgc aggtcgtcca ggcgggcaac
     4021 ctccgctccg gcctcctcct gctcggcctg gcccgggacc ggggtcggct ggaccgggtg
     4081 atcctcggca ccgacacccc cagcggcacc ggggtcatgc ccctgg
//
LOCUS       DASSWR010000008         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1384964, whole genome shotgun sequence.
ACCESSION   DASSWR010000008 DASSWR010000000
VERSION     DASSWR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384964"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1285)
                     /locus_tag="VFO47_00125"
     CDS             complement(125..1285)
                     /locus_tag="VFO47_00125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8787135.1"
                     /translation="MSEADREIQAAARALADELLPHEVEAELHGGELPDGLLEAHRRR
                     VAELGFEAINMPKELGGGGFTAFQQVLVQEQIGRVTNALGWVVHTPASWLPKVATTEQ
                     LDRWVVPTIRGERRECYAITEEGAGSDVDAIQATATRDGDDYLLNGVKWHVTSFNHAD
                     HVFFQGKLAGGANAGAHAMFVLDLDTPGIRVVRTPAYTHTYPYHHPVVAFEDVRVPAG
                     QLIGAEGDGMTFAYEWFRYERLMIAARALGAAERLIEEATAFARERHAYGQAIFGYQA
                     VQFMLADSLTELWAARLMTYQTAHGIDRDADVKVQHAQCSMAKLYASEMAGRVADRAV
                     QIFGGRGYMRENVAERFYRELRVDRIWEGTSEIQRAIIADQLAKRGVGPLTG"
     gene            1394..2635
                     /locus_tag="VFO47_00130"
     CDS             1394..2635
                     /locus_tag="VFO47_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8787136.1"
                     /translation="MARMSRRAFLSRSAATALAVSGGVLLSACGERDATGVAPGAGGP
                     KLASPENPVTWPVPADAGRIADGLAPEQGATLRVYNWSEYIYKKVVQDFEKKYKKYDV
                     KVQVSTYGNMDEAVAKLRAGQVAFDVLFPTYDYIGKLIQGNFLRPLNHSYIPNIEQVW
                     PAFQNPWYDQGWNYTVPYTIYTTGIAWRVDKVTDDVAKLANPYEALWDERYRGKVGIL
                     DDYREAIGMTLLKNGITDLNTANQDHLNLARDQLVDLAQTVRPRVNTNDYIDLPESKT
                     WITQAWSGDMVNAQYYMPKGQSADVMRYWFPADGKGPVNSDLIVLLRSGKNPVLGHLF
                     MDHLLDYDVAVENMSWNGYQPPQNKLNPTELVGQELLPKNLETATVLPAYFDSGYRYL
                     ELPGEVDDRWHAVWQEFKAGA"
     gene            2786..>3576
                     /locus_tag="VFO47_00135"
     CDS             2786..>3576
                     /locus_tag="VFO47_00135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787137.1"
                     /translation="MVWLVVLFLLPFYVVLSIAFGAVDPLFRTPVPVWNPAQWNPFQF
                     ELLYDRVFGPGGFLGPAFLRTFAYVAAASALCLLVAYPVAYYVTRYAGRRKGLLLVAL
                     LAPFWVSYMMRMLAWVNLLEADGMVNNVLEAVGILDEPFPWLAGQPVTVTLGLVYGYI
                     PYMVLALYAGLDRVDQRLLEAARDLGASRRRTFVLVTLPLSRQAILAGLVVTSLPMFG
                     DYFTNDLLSGSPRTSMVGNLVNDAIASPGQGGQAAALVLVMMALLA"
BASE COUNT          527 a         1288 c         1204 g          557 t
ORIGIN      
        1 gccggccagc gacccggcgg cccgctcgag cagggcccgg gagcggggct ggcgctccag
       61 gaacagcttc tcctggactg cgatcagctc gtcaaggcgg tccgcgaccg tcatacgtcg
      121 tctcctatcc ggtgagcggg ccgacgccgc gcttggcgag ctggtcggcg atgatcgccc
      181 gctggatctc cgaggtccct tcccagatcc tgtccacccg aagctcccgg tagaaccgct
      241 cggcgacgtt ctcgcgcatg tagccgcggc cgccgaagat ctgcaccgcc cggtcggcga
      301 cccggccggc catctcggag gcgtacagct tggccatcga gcactgggcg tgctggacct
      361 tgacgtcggc gtcgcggtcg atgccgtggg cggtctggta ggtcatcagc cgggccgccc
      421 acagctcggt cagggagtcg gccagcatga actggaccgc ctggtagccg aagatggcct
      481 ggccgtaggc gtggcgctcc ctggcgaagg ccgtggcctc ctcgatcagc cgctcggccg
      541 cgcccagggc ccgggcggcg atcatcagcc gctcgtaccg gaaccactca taggcgaagg
      601 tcatgccgtc gccctcggcg ccgatcagct ggccggcggg gacgcggacg tcctcgaagg
      661 cgaccaccgg gtggtggtag gggtaggtgt gggtgtaggc gggggtgcgg accacccgga
      721 tgccgggggt gtcgaggtcg agcacgaaca tggcgtgggc gccggcgttg gccccgccgg
      781 ccagcttgcc ctggaagaac acgtggtcgg cgtggttgaa ggaggtcacg tgccacttga
      841 ccccgttgag caggtagtcg tcgccgtcgc gggtggccgt ggcctggatg gcgtcgacgt
      901 cggagccggc gccctcctcg gtgatggcgt agcactcgcg gcgctcgccg cggatggtcg
      961 gcaccaccca gcggtccagc tgctcggtgg tggcgacctt gggcagccag gaggccgggg
     1021 tgtggaccac ccagccgagg gcgttggtga cccgcccgat ctgctcctgg accagcacct
     1081 gctggaaggc cgtgaagccg cccccgccga gctccttggg catgttgatg gcctcgaagc
     1141 cgagctcggc gacccggcgc cggtgcgcct cgagcagccc gtcgggcagc tcgccgccgt
     1201 gcagctcggc ctcgacctcg tgggggagca gctcgtccgc gagcgccctg gcggccgcct
     1261 ggatctcccg gtctgcctcc gacatcggat agcttcggcc cactaacaga ctccccggaa
     1321 gcgtaacgca aacattgact gaacgttcag tttattgtta gcttccgccc atctgcaagc
     1381 tcaggaggca gccatggcca ggatgagccg ccgagccttc ctctcccgtt ccgccgccac
     1441 cgcgctggcc gtctccggag gagtcctgct gtcggcgtgc ggcgagcgcg acgccaccgg
     1501 ggtcgccccc ggcgccggcg ggcccaagct ggccagcccc gagaacccgg tcacctggcc
     1561 ggtcccggcc gacgccgggc gcatcgccga cggcctggcc ccggagcagg gcgccaccct
     1621 gcgcgtctac aactggagcg agtacatcta caagaaggtc gtccaggact tcgagaagaa
     1681 gtacaagaag tacgacgtca aggtgcaggt ctccacctac ggcaacatgg acgaggccgt
     1741 ggccaagctg cgggccggcc aggtcgcctt cgacgtgctg ttccccacct acgactacat
     1801 cggcaagctg atccagggga acttcctgcg ccccctcaac cactcctaca tccccaacat
     1861 cgagcaggtc tggccggcgt tccagaaccc ctggtacgac cagggctgga actacacggt
     1921 gccgtacacg atctacacca ccggcatcgc ctggcgggtc gacaaggtca ccgacgacgt
     1981 cgccaagctg gccaacccct atgaggccct ctgggacgag cggtaccgcg gcaaggtcgg
     2041 catcctcgac gactaccgcg aggccatcgg catgaccctg ctcaagaacg gcatcaccga
     2101 cctcaacacg gccaaccagg accacctcaa cctggcccgg gaccagctgg tggacctggc
     2161 ccagacggtc cggccgcggg tcaacaccaa cgactacatc gacctgcccg agtccaagac
     2221 ctggatcacc caggcctggt cgggcgacat ggtcaacgcc cagtactaca tgcccaaggg
     2281 ccagagcgcc gacgtgatgc gctactggtt cccggccgac ggcaagggtc cggtcaacag
     2341 cgacctgatc gtgctgctgc gctcgggcaa gaacccggtg ctcggccacc tgttcatgga
     2401 ccacctgctc gactacgacg tggccgtgga gaacatgagc tggaacggct accagccgcc
     2461 ccagaacaag ctcaacccga ccgagctggt cggccaggag ctgctgccca agaacctcga
     2521 gaccgcgacc gtgctgcccg cctacttcga ctccggctac cgctacctgg agctgcccgg
     2581 cgaggtcgac gaccgctggc acgcggtctg gcaggagttc aaggccggag cgtagccggt
     2641 ggcgacctcg gaggccccgg cgacgacccg tgagcccggt caggccgagc cgaagttgcc
     2701 gcaccggcgg ggtgcgcgtc gcgcctccgc ccgtgccggc gcctggcacc cccgctggtt
     2761 ctggccgtcc ttcgccgtcc cggggatggt ctggctggtc gtgctgttcc tgctgccgtt
     2821 ctacgtggtc ctgtcgatcg ccttcggggc cgtcgacccg ctgttccgga ccccggtgcc
     2881 ggtctggaat ccggcccagt ggaacccgtt ccagttcgag ctgctgtacg accgcgtctt
     2941 cggccccggc gggttcctcg gcccggcgtt cctgcgcacc ttcgcctatg tggccgcggc
     3001 cagcgccctc tgcctgctgg tggcctaccc ggtcgcctac tacgtgaccc gctatgccgg
     3061 ccgccgcaag gggctgctgc tggtggccct gctggccccg ttctgggtca gctacatgat
     3121 gcgcatgctg gcctgggtca acctcctgga ggccgacggc atggtcaaca acgtgctgga
     3181 ggcggtcggg atcctggacg agccgttccc gtggctggcc ggccagccgg tcaccgtcac
     3241 cctggggctg gtctacggct acatcccgta catggtcctg gccctgtacg ccgggctgga
     3301 ccgggtcgac cagcggctgc tggaggcggc ccgcgacctg ggggcgagcc ggcggcggac
     3361 cttcgtgctg gtcaccctgc ccctgtcccg ccaggccatc ctggccggcc tggtcgtcac
     3421 ctccctgccc atgttcggcg actacttcac caacgacctg ctgtcggggt cgccgcggac
     3481 ctcgatggtc ggcaacctgg tcaacgacgc catcgccagc cccggccagg gcggccaggc
     3541 cgccgccctc gtgctggtca tgatggccct gctggc
//
LOCUS       DASSWR010000009         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_56870,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000009 DASSWR010000000
VERSION     DASSWR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56870"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VFO47_00140"
     CDS             <1..1407
                     /locus_tag="VFO47_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET8787138.1"
                     /translation="AWLPQESPPTTASTGLARLLSARGLVRARHELEEARRRIEQSAD
                     ARARDRAIKRFSGLQERFEAEGGYRAESEARRVAAGLGIPASGLDRPLSGLSGGERRR
                     VELARVLLSQAATLLLDEPTNHLDADAKRWLAGFLADFSGGILLVSHDLPLLDRDVTG
                     VLVLDPLTASIETYKGTWTQYLEAREVRARSEAKQRKVLERDIKRLSAFVEKYRHSNE
                     DMARRAMVTERRVEKMKQRLTPERKSGRKVAVRFPDPPPSGRFPLDAVDLSRSYGGPP
                     VFKHVSFDLQRGERLLVLGLNGAGKTSLLRILAGIDQPDEGEVRRGHGAELGYYAQEH
                     EGLDPAATAMESLREVAKAPDDLLRGVLGHFLLGGDQAHQPTRTLSGGEKTKLALARL
                     VLGRHNVLLLDEPTNNLDVQAVEAVRSALETYKGSVVLVSHDTPFVQSFEPDRVLLMP
                     DGALDVWSEDYLDLVALD"
     gene            complement(1555..1947)
                     /locus_tag="VFO47_00145"
     CDS             complement(1555..1947)
                     /locus_tag="VFO47_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET8787139.1"
                     /translation="MPVQRFAQVFGAVYVLVGLLGFAFTGLDGFAAPSDDKLLLFGVN
                     PLHNIVHLAVGALWLASSRTESSARAVSTLIGAVYLVVGILGLFINGSSDLNLLNINQ
                     PDNALHLASAALGLYFGLAGRRTAAPAA"
BASE COUNT          307 a          793 c          780 g          267 t
ORIGIN      
        1 gcctggctgc cgcaggagtc gccgccgacc acggcctcga ccgggctggc ccggctgctg
       61 tcggctcggg ggctggtccg ggcccggcac gagctggagg aggctcgacg gcggatcgag
      121 cagagcgccg acgcccgggc ccgcgaccgg gccatcaagc ggttctcggg cctccaggag
      181 cgcttcgagg ccgagggcgg ctaccgggcc gagtccgagg cccggcgggt cgcggccggc
      241 ctcggcatcc cggccagcgg gctcgaccgg ccgctgtcgg ggctgtcggg cggcgagcgg
      301 cgccgggtcg agctggcccg ggtgctgctc agccaggcgg ccacgctgct gctggacgag
      361 cccaccaacc acctggacgc cgacgccaag cgctggctgg ccgggttcct ggccgacttc
      421 tccggcggca tcctgctcgt ctcccacgac ctgcccctgc tcgaccggga cgtcaccggg
      481 gtgctggtgc tcgacccgct caccgcctcg atcgagacct acaagggcac ctggacccag
      541 tacctggagg cgcgcgaggt ccgggcccgg tccgaggcca agcagcgcaa ggtcctggag
      601 cgcgacatca agcggctgtc ggccttcgtg gagaagtacc gccactccaa cgaggacatg
      661 gcccgccggg ccatggtcac cgagcggcgg gtcgagaaga tgaagcagcg gctcaccccg
      721 gagcgcaaga gcggtcggaa ggtggccgtc cgcttccccg acccgccgcc gtcgggccgc
      781 ttcccgctgg acgcggtcga cctgtcccgc tcctacggcg gcccgccggt gttcaagcac
      841 gtctccttcg acctccagcg gggcgagcgg ctgctcgtgc tggggctgaa cggggccggc
      901 aagacctcgc tgctgcgcat cctggccggc atcgaccagc ccgacgaggg cgaggtccgc
      961 cgcggccacg gcgccgagct gggctactac gcccaggagc acgagggcct cgacccggcc
     1021 gccaccgcca tggagtcgct gcgggaggtc gccaaggccc ccgacgacct gctccggggc
     1081 gtcctcggcc acttcctgct cggcggcgac caggcccacc agccgacccg gaccctgtcc
     1141 ggcggcgaga agaccaagct cgccctggcc cggctggtcc tgggccgcca caacgtcctg
     1201 ctcctggacg agcccaccaa caacctggac gtccaggcgg tcgaggccgt ccgctcggcc
     1261 ctggagacct acaagggctc ggtggtcctg gtcagccacg acaccccgtt cgtgcagagc
     1321 ttcgagcccg accgggtcct gctcatgccc gacggcgccc tggacgtctg gagcgaggac
     1381 tacctggacc tggtggccct cgactgagtg gtcgcgacgc cgcccggccc ggcgcaggcg
     1441 ctcgcgcagg tcgagccgcc ggtcgaggcg gagcgggtag acctccgcat cacgagaaca
     1501 agagaggggc cgagggatcc ccccggaatc cctcggcccg cctggccccc cggcctaggc
     1561 cgccggcgcg gccgtccgcc gcccggccag cccgaagtac aggccgagcg cggcgctcgc
     1621 caggtgcagc gcgttgtcgg gctggttgat gttgaggagg ttcaggtccg aggagccgtt
     1681 gatgaacagg cccaggatcc cgaccaccag gtagacggcg ccgatcaggg tgctgacggc
     1741 gcgggcgctc gactcggtgc gggacgaggc cagccacagg gccccgaccg cgaggtggac
     1801 gatgttgtgc agcgggttca ccccgaacag caggagcttg tcgtccgagg gggcggcgaa
     1861 gccgtccaac cccgtgaagg cgaacccgag caggcctacg agcacgtaga cggccccgaa
     1921 gacctgggcg aaacgctgga cgggcatgat ccgcatgacc tccttggcgg ctgtggagcc
     1981 ccggcgttgc cggctcacca tgcgttcgct gcgccgcctc gatcggttca tcggcgaggg
     2041 aggaatttct tgcgctcgcg aaccgtcccg accccaggag cgagccgtgg ggctggcctc
     2101 gccgaggagc gggacgtggc cgccgccgat ccccgccaag cctccga
//
LOCUS       DASSWR010000010         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_424208,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000010 DASSWR010000000
VERSION     DASSWR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424208"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFO47_00150"
     CDS             <1..594
                     /locus_tag="VFO47_00150"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HET8787140.1"
                     /translation="EVGFAEGEAGLVAYKLVPNFRALGPRFGRDAQAVAAALRQAPAT
                     LAAELAPRLRAGERVELAVDGLGAVELGPEEVTVAEEPVTGWRVVRDGATAVALDLEL
                     SPELRREGLARDLVRAVQDLRKAAGLAVDDRIELAVKADGEAAAAVAAHRDHLLGETL
                     ATSLHGAPQGDGHDARVDLDGQEVRIWLRPVREGAWG"
     gene            591..1067
                     /locus_tag="VFO47_00155"
     CDS             591..1067
                     /locus_tag="VFO47_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787141.1"
                     /translation="MSGRGPTDPPPARGPGRLDDLGDLLPAARSLWRYVWAGPNSLVG
                     LVGGLTTGTRPVRWRGVLLFEDARGGLARFLRWRGFAAITLGHVIVTNRRLNDGLLAH
                     ELEHVAQAERWGPLYYPAYLLGSVRGYRRNPFERAARRAADTVLAALEERTKGRRG"
     gene            complement(1068..1229)
                     /locus_tag="VFO47_00160"
     CDS             complement(1068..1229)
                     /locus_tag="VFO47_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787142.1"
                     /translation="MVAGALLALALSLGLLVASLFRTDGLGLVWTSLAIDLVALTLLV
                     TALRRRRAR"
     gene            complement(1273..>2166)
                     /locus_tag="VFO47_00165"
     CDS             complement(1273..>2166)
                     /locus_tag="VFO47_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET8787143.1"
                     /translation="TPLDIQHKEFTKAMRGYAMHEVDTFLDQVTEEFTRMQDEIARLR
                     EQASSAAPPQPAPVVQQAPPPPPPQPQPVAAESRGGAGGEEAIARALVMAQRMADQTV
                     EEAKVKAKSMVAEAEARAKNMTEQAQMRAREVTEAAQMRAREVTEAAQARARELTEGL
                     ETRYKERIQSAEARARVAEEQARMQIAQATEQVARRRQELESSIEALRAFERDYRARL
                     RGFVEGQLKALEDAAPSGPVAPPQPPGLGGNSRPLPGAEDLPALSTGARQSSYSALRD
                     SSSDQLGHNGRDRVDRLRHDD"
BASE COUNT          232 a          782 c          872 g          280 t
ORIGIN      
        1 gaggtcggct tcgccgaagg cgaggccggt ctggtcgcct acaagctggt cccgaacttc
       61 cgagcgctgg ggccccggtt cgggcgcgac gcccaggcgg tggccgccgc tctgcgccag
      121 gccccggcca ccctcgccgc cgagctggcc ccgcggctgc gggccggcga gcgggtcgag
      181 ctggccgtgg acgggctggg cgcggtcgag ctggggccgg aggaggtgac ggtggccgag
      241 gagccggtga ccggctggcg ggtggtccgc gacggcgcca ccgcggtcgc cctcgacctg
      301 gagctctcgc ccgagctgcg ccgcgaggga ctggcccgcg acctggtccg ggccgtgcag
      361 gacctgcgca aggcggccgg gctggccgtc gacgaccgga tcgagctggc cgtcaaggcc
      421 gacggcgagg ccgccgcggc ggtggccgcc caccgcgacc acctgctcgg cgagaccctg
      481 gccaccagcc tccacggcgc cccccagggc gacggccacg acgcccgggt cgacctggac
      541 ggccaggagg tccgcatctg gctgcggccg gtccgggagg gggcgtgggg gtgagcggcc
      601 gcgggccaac tgatccacct cccgcccggg ggccggggcg tctggacgac ctgggggacc
      661 tgctgccggc ggcgcggtcg ctgtggcggt acgtctgggc cgggccgaac agcctggtcg
      721 ggttggtggg cgggctcacc accgggaccc ggccggtgcg ctggcggggg gtgctgctgt
      781 tcgaggacgc ccgcgggggc ctggcccggt tcctgcgctg gcgcgggttc gcggccatca
      841 ccctcgggca cgtgatcgtg accaaccggc gcctgaatga cgggctgctc gcccacgagc
      901 tggagcacgt cgcccaggcc gagcgctggg ggccgctgta ctacccggcc tacctgctcg
      961 ggagcgtccg cgggtaccgg cgcaacccgt tcgagcgcgc cgcccggcgg gcggccgaca
     1021 ccgtcctggc cgccctggag gagcgcacca agggccggcg gggctagtca gcgggcccgg
     1081 cggcggcgca gggccgtcac cagcaaggtg agggcgacaa ggtcgatggc cagcgacgtc
     1141 cagacgaggc cgaggccgtc ggtgcggaac agcgacgcga ccagcaggcc cagggacagt
     1201 gccagggcga gcagggcgcc ggcgaccacc gggctcgcta cgggaccgtt ctagcgatga
     1261 ccgtggcggc cgtcagtcgt cgtgccgcag gcggtcgacg cggtcgcggc cgttgtggcc
     1321 gagctggtcc gagctggagt cgcgcagggc cgagtaggag ctctggcgcg ccccggtcga
     1381 cagggccggc aggtcctcgg cgcccggcag gggccgggag ttcccgccga ggcccggcgg
     1441 ctgcggcggg gccacgggcc cggacggggc cgcgtcctcg agcgccttga gctggccctc
     1501 gacgaagccg cggagccggg cgcggtagtc gcgctcgaac gcgcgcaggg cctcgatcga
     1561 gctctccagc tcctggcggc gccgggccac ctgctcggtc gcctgggcga tctgcatgcg
     1621 ggcctgctcc tcggccacgc gggcgcgggc ctcggccgac tggatccgct ccttgtagcg
     1681 ggtctccagg ccctcggtga gctcacgggc cctggcctgg gcggcctcgg tgacctcacg
     1741 ggcccgcatc tgggcggcct cggtgacctc acgggcccgc atctgggcct gctcggtcat
     1801 gttcttggcc cgcgcctcgg cctcggcgac catcgacttg gccttgacct tggcctcctc
     1861 gaccgtctgg tcggccatcc gctgcgccat caccagggcc cgggcgatcg cctcctcgcc
     1921 acctgcgccg ccgcgggact cggcagccac cggctggggc tgcggaggcg ggggcggggg
     1981 cgcctgctgc accaccggcg ccggctgggg tggcgccgcg ctggaggcct gctcccggag
     2041 gcgggcgatc tcgtcctgca tccgggtgaa ctcctcggtg acctggtcga ggaaggtgtc
     2101 cacctcgtgc atggcgtacc cgcgcatggc cttggtgaat tctttgtgtt ggatgtccag
     2161 cggggt
//
LOCUS       DASSWR010000011         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_169963,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000011 DASSWR010000000
VERSION     DASSWR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169963"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..817)
                     /gene="cmk"
                     /locus_tag="VFO47_00170"
     CDS             complement(101..817)
                     /gene="cmk"
                     /locus_tag="VFO47_00170"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET8787144.1"
                     /translation="MRVVGLGGDEPVEGTADVVVAVDGPAGSGKSTVARRVAERAGLR
                     YLDTGATYRALTLALLRRGVPVDDPEAVADAAKAVDLTLELPPGRAGAARVLLDGTEP
                     GPALRTPEVNATVSAVSAVPEVRELLVGLQRSLVGVGGIVVEGRDIGTVVWPDADVKV
                     FLTASEHERARRRTGDAEGGGETAETLAHRDALDSGRAASPTRPAADALIIDSTTRSI
                     DQVVDQLLELIEAARASRPR"
     gene            complement(1111..>2095)
                     /locus_tag="VFO47_00175"
     CDS             complement(1111..>2095)
                     /locus_tag="VFO47_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET8787145.1"
                     /translation="RDALAREAITSRARSVEEAAEGADLIVAATPVRSLAGVLARAHA
                     ANPEAVLTDVGSTKLHLLAELQRSSTNLRRVIPGHPMAGSEERGAGAARVDLFHGAAW
                     VLTPAPHVDHQALRRLTSFVRDLGGRPLMLDPELHDQVAAFASHLPQLAATALMGAVA
                     QVESPAALRRLVASGFRDTTRVAASDPDLWVDICATNGPSIVAALDVLTSRLEVLRKL
                     IAEGDRAGLREELASARTARLRIPTKPGASPRGLREVVVHIADRPGSLAAVFHALGAA
                     GVNVEDLAIDHELQGGSGALRIWVAGREALTTAIAALEMAGWPAHEGTGAP"
BASE COUNT          239 a          802 c          790 g          264 t
ORIGIN      
        1 ttctcggagc cgacgggccc accggcgtcg gcgggtccgg tgcctccgcc gtcggcgtcg
       61 gcggccgggg cgcggggcgc ctcgcggtgg ggaccgccgg tcatcgcggg cggctggctc
      121 tggcggcttc gatgagctcc aggagctggt ccaccacctg gtcgatggag cgggtggtgg
      181 agtcgatgat gagggcgtcg gcggccgggc gggtcgggga ggcggcccgg ccgctgtcga
      241 gggcgtcgcg gtgggccagg gtctcggcgg tctcgccgcc gccctcggcg tcgccggtgc
      301 gccgccgggc ccgttcgtgc tcggaggcgg tgaggaacac cttgacgtcg gcgtcgggcc
      361 agaccacggt cccgatgtca cggccctcga ccacgatgcc cccgaccccg accagcgacc
      421 gctgcagccc gaccagcagc tcacggacct ccgggacggc ggagacggcc gacacggtgg
      481 cgttgacctc cggggtgcgg agcgccggtc ccggctcggt cccgtccagc agcaccctgg
      541 ccgccccggc ccggccaggc ggcagctcga gggtgaggtc gaccgccttg gccgcgtcgg
      601 ccaccgcctc cgggtcgtcg acggggacac cccggcgcag cagcgccagg gtgagcgcgc
      661 gataggtcgc cccggtgtcc aggtagcgca ggccggcccg ctcggcgacc cgccgggcga
      721 ccgtgctctt gcccgacccg gccggcccgt cgacggcgac caccacgtcg gcggtgccct
      781 cgaccggctc gtcgccgccc aacccgacca ccctcacctg gcctccctac tcgaccagct
      841 cacccagctc acctgcacct cgctcatcac ccggcctgtc ccggcgcgcg aggggcaagg
      901 gccccgcgtc ggccgccccg ggctcccggc cgccctggcc ggcgcgggca catcgcatgg
      961 atccctggtc cgggacctgt gcccatccgc agcgcagtct acccgctggc ggccctgcac
     1021 cctcaccgtc gtcgctcacc ggagcgtcgg gaatcgccat gtctatctat cgatggatag
     1081 gtagatctat tgatccacag tgtgctccgg tcagggggcg ccggtgccct cgtgggcggg
     1141 ccagccggcc atctccaggg cggcgatggc ggtggtgagg gcctcccggc cggcgaccca
     1201 gatccgcagg gcgccgctcc ccccctgcag ctcgtggtcg atggccaggt cctcgacgtt
     1261 gaccccggcc gcgcccaggg cgtggaacac ggcggccagg ctgcccggcc ggtccgcgat
     1321 gtggaccacc acctcccgca gcccgcgggg cgaggcgccg ggcttggtcg ggatgcgcag
     1381 gcgcgccgtc cgggcgctgg ccagctcctc gcgcagtccg gcccggtccc cctcggcgat
     1441 gagcttccgc agcacctcga gccggctggt cagcacgtcc agcgcggcca cgatgctcgg
     1501 gccgttggtg gcgcagatgt cgacccacag gtccgggtcg ctggcggcca cccgcgtggt
     1561 gtcgcggaag ccgctggcga ccaggcggcg cagggcggcc ggcgactcca cctgggccac
     1621 cgcgcccatg agggcggtcg cggccagctg cggcagatgg ctcgcgaacg ccgccacctg
     1681 gtcgtgcagc tccgggtcga gcatgagcgg ccgcccgccg aggtcccgca cgaacgaggt
     1741 cagccggcgc agcgcctggt ggtcgacgtg cggggccggg gtcagcaccc aggccgcgcc
     1801 gtggaacagg tcgacccggg cggcgcccgc cccccgctcc tcggacccgg ccatggggtg
     1861 gccggggatg acccgacgca gattggtgga tgaacgttga agctcagcta gaaggtgcaa
     1921 cttggtactg ccgacgtcgg tgagcaccgc ctccgggttc gccgcgtggg cgcgggcgag
     1981 cacgccggcc agcgagcgga ccggggtcgc ggccacgatc aggtcggcgc cctcggccgc
     2041 ctcctcgacg gagcgggccc ggctggtgat cgcctcccgt gccagggcgt cgcgc
//
LOCUS       DASSWR010000012         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1243778, whole genome shotgun sequence.
ACCESSION   DASSWR010000012 DASSWR010000000
VERSION     DASSWR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243778"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFO47_00180"
     CDS             complement(<1..223)
                     /locus_tag="VFO47_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787146.1"
                     /translation="MALAAQRNQVTERGRRRVAGALVTGAALLVVYWAAWLLDRTLLG
                     SDTRPAYYEFQAAFVLADAWLAFCLVAGAR"
     gene            complement(298..846)
                     /gene="lepB"
                     /locus_tag="VFO47_00185"
     CDS             complement(298..846)
                     /gene="lepB"
                     /locus_tag="VFO47_00185"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET8787147.1"
                     /translation="MAALILTILVKGFLIQAFFIPSRSMEPTLDVGDRVVVNRLAYRL
                     GDPAHGQVVVFLRPTGADQPPASGPISWVRRAVAQGLGGTPPGNEDLIKRVVGLPGDE
                     LEGRDGHLIRNGAVVDEPYLRPGTFTSDFDKVRIKPDHLWVMGDNREDSADSRSFGQV
                     HRSALVGRAVLTVWPVQHTGGL"
     gene            complement(1039..2499)
                     /locus_tag="VFO47_00190"
     CDS             complement(1039..2499)
                     /locus_tag="VFO47_00190"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8787148.1"
                     /translation="MRTAMWVGGVELPGRGAVEVVANPATEEPLAEVPSASPEQVDAA
                     VEAARGALPGWRRTPAGERGELLHGVAAWLREHTDELGRLMALEGGKPLVENTDEVGW
                     TANAFTYYAELGRHERGRVIPSVEEGQLSLVLKEPVGVVATIVPWNYPLLLLAWKVAP
                     ALAAGNTVVVKPSPYTPLATLRLAEAFAGLPDGVVNLVTGGAEVGEALVVHGGTSLVA
                     FTGSVETGQRIGALCAERVKRTHLELGGKDAFIVCDDVDLAVAAPGAAWAAYLNAGQV
                     CTSAERFYVMEPVFDEFVDRLVTVTKGLRLGDPLDPATDVGPMVAEAQRAKVERQLGG
                     AVAAGARVLAGGDHGGQQRGWFLAPTVVVDVDDTMDLLTTETFGPVAPVQRVGSLDEA
                     IARANALPYGLGANVYTGRMDYVLRCMEELEAGTVWFNDPLTDNDAGPFGGMKMSGNA
                     RELGPEGLEAFRETKHVHLDARANRKPWWYPYGQEG"
BASE COUNT          354 a          955 c          928 g          333 t
ORIGIN      
        1 cccgggcgcc cgcgaccagg cagaaggcca gccaggcgtc ggccaggacg aacgccgcct
       61 ggaactcgta gtaggcgggc cgggtgtcgc tgcccagcag ggtccggtcg agcagccagg
      121 ccgcccagta gacgaccagc agggccgcgc cggtcaccag ggcgccggcg acgcgtcgcc
      181 gtcctcgctc ggtgacctgg ttccgctggg ccgccagcgc catccagccg tctccttcct
      241 cccgccaggc acaggtaatc ttgccccctt gcccccacaa gagggaaaac gacaggtcta
      301 gaggccgccg gtgtgctgca ccggccagac ggtgagcacg gcccggccga ccagggccga
      361 gcggtggacc tggccgaagc tcctggagtc ggccgagtcc tcgcggttgt cgcccatcac
      421 ccagaggtgg tctggcttga tccgcacctt gtcgaagtcg gaggtgaagg tgccggggcg
      481 gaggtagggc tcgtcgacca cggcgccgtt gcggatcagg tggccgtcgc ggccctcgag
      541 ctcgtcgccg ggaagcccga cgacccgctt gatcaggtcc tcgttgcccg gcggggtgcc
      601 tccgagcccc tgggcgacgg cccgccggac ccaggagatg ggtccgctcg ccgggggctg
      661 gtcggcgccg gtcgggcgca ggaagaccac cacctggccg tgggcggggt cgccgaggcg
      721 gtaggcgagc cggttgacga ccacacggtc gcccacgtcc agggtcggct ccatcgaccg
      781 cgacgggatg aagaaggcct ggatcaggaa ccccttgacc aggatggtca ggatcagggc
      841 ggccaccagc aggaacggca gctcggcaaa gaaccggcgc cgggtccgcc gggcagcgtt
      901 acggcggccg tggatgcgct cgacgcggga gggctggacg cggatcgtct ggctcagcgc
      961 ccgacctccg gggaccggag tcttgacatg ccgcctccat cggccggaca ggccgtcggc
     1021 ttgacgcggt ccaccaggct aaccctcctg gccgtaggga taccaccacg gcttgcggtt
     1081 ggcccgggcg tcgaggtgga cgtgcttggt ctcgcggaac gcctccaggc cctccggtcc
     1141 cagctcgcgg gcgttgccgg acatcttcat gccgccgaac gggccggcgt cgttgtcggt
     1201 gagggggtcg ttgaaccaga cggtgcccgc ctccagctcc tccatgcagc gcagcacgta
     1261 gtccatccgg cccgtgtaga cgttggcccc cagcccgtag gggagggcgt tggcccgcgc
     1321 gatcgcctcg tccagcgagc cgacccgctg cacgggcgcg accggcccga aggtctcggt
     1381 ggtgagcagg tccatggtgt cgtccacgtc caccaccacc gtcggggcca ggaaccagcc
     1441 ccgctgctgc ccgccgtggt ccccgccggc caggacccgg gccccggccg ccaccgcccc
     1501 gccgagctgg cgctccacct tggcccgctg ggcctcggcc accatcgggc cgacgtcggt
     1561 ggccgggtcg agcgggtcgc ccagccgcag ccccttggtc accgtgacca ggcggtcgac
     1621 gaactcgtcg aacaccggct ccatgacgta gaagcgctcg gccgacgtgc agacctggcc
     1681 cgcgttcagg taggccgccc aggcggcgcc gggggcggcg accgccaggt cgacgtcgtc
     1741 gcagacgatg aacgcgtcct tgccgcccag ctccaggtgg gtccgcttga cccgctcggc
     1801 gcagagggcg ccgatgcgct ggccggtctc caccgacccg gtgaaggcga ccaggctggt
     1861 cccgccgtgc acgaccaggg cctcgccgac ctcggccccg ccggtgacca ggttgaccac
     1921 gccgtcgggg agcccggcga acgcctcggc caggcgcaag gtggccagcg gcgtgtacgg
     1981 cgacggcttg accaccaccg tgttgccggc ggccagggcg ggggcgacct tccaggccag
     2041 caggagcagc gggtagttcc agggcacgat cgtggccacg accccgaccg gctccttcag
     2101 caccagcgag agctggccct cctcgaccga ggggatgacc cggccgcgct cgtggcgtcc
     2161 cagctcggcg tagtaggtga aggcgttggc cgtccagccg acctcgtcgg tgttctcgac
     2221 cagtggcttg ccgccctcca gggccatcag gcggccgagc tcgtcggtgt gctcgcgcag
     2281 ccaggcggcg accccgtgga gcagctcgcc gcgctcgccg gccggggtcc gccgccagcc
     2341 gggcagcgcg cccctggccg cctccacggc cgcgtcgacc tgctcgggcg aggccgacgg
     2401 cacctcggcc agcggctcct cggtggccgg gttggcgacc acctcgaccg cgccccggcc
     2461 gggcagctcg acgccgccaa cccacatggc agtcctcatg cagacactgt agccagggcc
     2521 tggtcgagga tgtcgagccc ctcggccagc agccgctcct ccatgacgag
//
LOCUS       DASSWR010000013         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_509066,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000013 DASSWR010000000
VERSION     DASSWR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509066"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..831)
                     /locus_tag="VFO47_00195"
     CDS             complement(274..831)
                     /locus_tag="VFO47_00195"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HET8787149.1"
                     /translation="MATILWQAVESGALERCRLEAGPDGLRLSGTVLTPEHGTPLDVR
                     YRVEAGPDGLTRRVELELDGGATRRVLLADGAGTWRWDGGPALPEVAGALDVDLTVTP
                     STNTLPIRRLARQLEPGQAADLRMAWVQFPGLEVIGSAQRYQRLATDRWQFSTGDFQA
                     DLLVDPDGLVLDYEGLFRSLAYRPG"
     gene            complement(840..2204)
                     /locus_tag="VFO47_00200"
                     /pseudo
     CDS             complement(840..2204)
                     /locus_tag="VFO47_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011455564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
     gene            2296..>2844
                     /locus_tag="VFO47_00205"
     CDS             2296..>2844
                     /locus_tag="VFO47_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787150.1"
                     /translation="MAQRISSPVLAGRATELARLRSATERAAAGTPVAVVVAGEAGVG
                     KTRLVAELAGEAEAAGMAVLGGGCLDVGDGTLPYAPLAEALRELAADLDPAELDGGLG
                     GARIELSRLVPELAAPTSAPGPPPGTSPGWLFELLLGVVHRLAARRPVLLVVEDLHWA
                     DRSTRDLLGYLVRNLRAGVALVL"
BASE COUNT          356 a         1019 c         1109 g          360 t
ORIGIN      
        1 gagaagggca tctcctactg gaccaagaac taccgcaccg gcatcgagtt cgacgacccc
       61 gacgccctca gccgccgcta cgagtactac gacccgatcc ggaccctgcc cgagtccggc
      121 aaggagtact ggcgcaagtt cggggtcccc gacaccgtcc cgatcagctg agggcgaact
      181 gctccaccga acgctacctc ccgacctggt cgccgaggcc ctgcgcgagg ccaccggcgc
      241 ggacgcggcc ctggtccggg tcctggaacg ggctcagccg gggcggtagg cgagggagcg
      301 gaacaggccc tcgtagtcga ggacgaggcc gtcggggtcg accagcaggt cggcctggaa
      361 gtcgccggtg gagaactgcc agcggtcggt ggcgaggcgc tggtagcgct gagctgatcc
      421 gatcacctcg aggccgggga actggaccca ggccatccgg aggtcggccg cctggccggg
      481 ctcgagctgg cgggccaggc ggcggatggg gagggtgttg gtggacgggg tgacggtcag
      541 gtcgacgtcg agggcgccgg caacctcggg cagggccggg ccgccgtccc agcgccaggt
      601 gccggccccg tcggcgagca ggacccgccg ggtggcgccg ccgtccagct ccagctcgac
      661 ccgccgggtc agcccgtccg gcccggcctc gacccggtag cggacgtcca ggggggtgcc
      721 gtgctcgggc gtgagcacgg tccccgacag ccgcagcccg tccggcccgg cctccagccg
      781 gcagcgctcc agggcgcccg actcgacggc ctgccagagg atcgtcgcca cgggcccgct
      841 cagccgcgca gcagctcggt gacctggaag gccaggtcca gggcctggct ggcgttgagg
      901 cgggggtcgc aggcggtctc gtagcggcgg tgcaggtgcc cgtcctcgat ctcgtcggcg
      961 ccgcccagac actcggtgac gtcctcgccg gtcagctcga cgtggatgcc ccccgggtgg
     1021 gtgccctcgg agcggtgcac ggcgaagaag ccggccagct cggccatgac gtcgtcgaag
     1081 cggcgggtct tgtggcccga cggcccgacg aaggtgttgc cgtgcatcgg gtcgcaggcc
     1141 cacacgaccg tgtggccggc gtcgcggacg gcccggacca gcggcggcag ccgctcctcg
     1201 acccgggcgg cgcccatcct ggccaccagg gtgagccggc ccgggacccg ccccgggtcg
     1261 agcaggtcgc acagggcgag gacctcctcg ggggtcgccg tcgggccgac cttggccccg
     1321 accgggttcc cgatgccgcg gaggaactcg aggtgggccc cgtccagctg ccgggtccgg
     1381 tcccccaccc acagcaggtg ggccgagcag tcgtaccagc acccggtgag gctgtcgcgc
     1441 ctggtcatgg cctcctcgta gccgagcagg agggcctcgt gggaggtgta gaagtcgacc
     1501 tggtggatga ccgaggaggc ggtcaggtcg atcccgcagg cggccatgaa gcgcagcgcc
     1561 cggtcgatgc cgtcggcgac cgcgtcgtag cgccgcccgg ccgggctgcg ggccacgaac
     1621 tcctggttcc agacgtgcac ctggttgagg tcggcgaacc cgcccttggt gaaggcgcgc
     1681 agcaggttca gggtggccga cgactggtgg taggcgcgca gcatccgggc cgggtcaggc
     1741 cggcgcgacg ccgggtcgaa gccgaggtcg ttgaccatgt cgccgcttta gacgggcagc
     1801 tcgaccccgt cgacccgctc gctggggagg ctgcggggct tggcgaactg gccggcgatg
     1861 cgcccgacct tggtcaccgg gacgccggcg ccgtaggtca ggacggcggc catctggagg
     1921 atgaccttga gcttgtcgcg gatcgtgtcg gccgagaagg cgtcgaacga ctcggcgcag
     1981 tcgcccgcct ggagcaggaa gccgtgcccg gaggccaccg tggccagggt ggactgcagc
     2041 gtcctggcct cgccggcgaa caccagcggc ggctggttcg acagctcctt caggacccgg
     2101 tcgagggcgt cgggctcggg ccagtcgggc tgctgggccg ccggcagcgc ccgccaggag
     2161 tcgggcgccc aggacgggga cgcggtgcga acggtggggt tcatcgctct atcgtcgcag
     2221 aaccgcgcat gatcgcgcat gcccggcgac agcgggcggt ctcgcccggc ggcggcgtct
     2281 ggcaagatcg gcggcgtggc ccagcgcatc tccagccccg tcctggccgg ccgcgccacc
     2341 gagctcgccc ggctccggtc cgccaccgag cgggcggcgg ccgggacccc ggtcgccgtg
     2401 gtcgtggcgg gcgaggccgg ggtcggcaag acccggctgg tcgccgagct ggccggcgag
     2461 gccgaggcgg ccggcatggc cgtgctcggc ggtggctgcc tcgacgtcgg cgacgggacg
     2521 ctgccgtacg cgccgctggc cgaggcgctc cgggagctgg ccgccgacct cgacccggcc
     2581 gagctggacg gcgggctcgg cggcgcccgc atcgagcttt cccgcctggt ccccgagctg
     2641 gccgcgccga cgtccgcccc cgggccgccg ccggggacgt cccccgggtg gctgttcgag
     2701 ctgctgctgg gggtcgtcca ccggctggcc gcccgccgcc cggtcctgct ggtggtcgag
     2761 gacctgcact gggccgaccg ctccacccgc gacctgctcg ggtacctggt gcgcaacctc
     2821 cgggccgggg tggccctggt cctt
//
LOCUS       DASSWR010000014         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_311343,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000014 DASSWR010000000
VERSION     DASSWR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311343"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFO47_00210"
     CDS             complement(<1..299)
                     /locus_tag="VFO47_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HET8787151.1"
                     /translation="MDTWGISGPRFLLLYVVLPAGMVLVAVVAGRRALAGPGEGAVAP
                     ARLDPYGAAYLNGGSGLVATTAVSGLLRGGRPATGIRRRGQVRLTARNAPEAGVHP"
     gene            506..1315
                     /locus_tag="VFO47_00215"
     CDS             506..1315
                     /locus_tag="VFO47_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8787152.1"
                     /translation="MPDRYAAYPSLETERPADGVLRVVMRAPGRLNAAGPQMHRDLAG
                     IWTEIDRDPEVRAVVVRGAGDAFSAGGDLDLVADMADDFELRVRVLREARDLVYNLLN
                     CSKPVVSAMAGPAVGAGLVIGLLADVSIAARSARIIDGHTRLGLAAGDHAAIVWPLLC
                     GMAKAKYHLLLCEPVSGEEAERIGLVSRCVDDAELHDVALEVAVRLAGGSQSAIRWTK
                     YALNNWLRLAGPTFDTSLALEFVGFGGPDVREGVASFREKRPPRFTGPASE"
     gene            complement(1379..2053)
                     /locus_tag="VFO47_00220"
     CDS             complement(1379..2053)
                     /locus_tag="VFO47_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8787153.1"
                     /translation="MTGPTKVLVVDDQGLVRAGFRMILEAQPDIEVVGEAADGLDAVE
                     AARRLRPDVVLMDIRMPRLDGLEATRRLAGPGTADPVRVLILTTFDLDEYVFEALRAG
                     ASGFLLKDLPREDLVAAGRVVAGGEALLAPRVTRRLIEEFARRPAGADPDPAALAALT
                     AREREALELMARGLSNAEMATSLFVSEHTVKTHVGNVLAKLGLRDRIQAVILAYEVGL
                     VQPGRG"
     gene            complement(2050..>2558)
                     /locus_tag="VFO47_00225"
     CDS             complement(2050..>2558)
                     /locus_tag="VFO47_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787154.1"
                     /translation="IAATGRQALVEMRRLLGVLRGDGDGDAAPSLAPQPGLAQVPSLV
                     EQVGRAGLRVELVVEGPEAPLPAGVDLSAYRIVQEALTNALRHGGAGRARVVVRYGEH
                     DLELQVTDEGRSNGSPPVGRSQGAQGRGLLGMKERAALFGGELHAGPAAAGGFAVLAR
                     LPIGDPAP"
BASE COUNT          309 a         1012 c          935 g          302 t
ORIGIN      
        1 gggtggaccc cggcctccgg agcgttccgg gcggtcagcc ggacctggcc gcggcggcgg
       61 atgccggtgg ccggccggcc gccccggagc aggccggaga cggcggtggt ggcgaccagc
      121 ccgctgccgc cgttgaggta ggcggccccg tacgggtcga ggcgggccgg ggcgaccgcc
      181 ccctcgcccg gcccggccag ggcccgcctc ccggccacga ccgccaccag caccatgccc
      241 gcgggcagga ccacgtacag gagcaggaac cggggaccgc tgatgcccca tgtgtccatc
      301 tggcgacctc ctggccgctc cgctggtcat ccccgccagc gcgctggtca tccctgccag
      361 cgtgtccgtt gccgtcacgg cgagtatcgg gccgcccggg gccgccgtcc agcctcccgg
      421 acaagttgtt accgcccggt aacccaccgt tcacgccacc ggcctgccac cattggccgg
      481 cacgtcgacg acccgaggag cagcgatgcc cgaccgctac gccgcctacc cgagcctgga
      541 gacggaacgg cccgccgacg gggtcctgcg ggtggtgatg cgcgcccccg gacgcctcaa
      601 cgcggccggc ccccagatgc accgcgacct ggccggcatc tggaccgaga tcgaccgcga
      661 ccccgaggtg cgcgccgtgg tcgtccgcgg ggccggggac gccttctcgg ccggcggcga
      721 cctcgacctg gtggccgaca tggccgacga cttcgagctc cgggtgcgcg tgctccgcga
      781 ggcccgcgac ctggtctaca acctgctcaa ctgctccaag ccggtggtct cggccatggc
      841 cgggccggcc gtcggggccg ggctggtcat cggcctgctg gccgacgtgt cgatcgccgc
      901 ccgctcggcc cggatcatcg acggccacac ccgcctgggg ctggccgccg gcgaccacgc
      961 ggcgatcgtc tggccgctgc tgtgcggcat ggccaaggcc aagtaccacc tgctgctgtg
     1021 cgagccggtg tcgggcgagg aggccgagcg gatcgggctg gtgtcgcgct gcgtggacga
     1081 cgccgagctg cacgacgtcg ccctggaggt ggcggtccgg ctggccggcg gctcccagag
     1141 cgccatccgc tggaccaagt acgcgctcaa caactggctg cggctggccg ggccgacctt
     1201 cgacacctcg ctggcgctcg agttcgtggg cttcggcggc ccggacgtgc gcgagggggt
     1261 ggcctcgttc cgagagaagc gcccgccccg cttcaccggc ccggcctccg agtaaggccc
     1321 ggccggtgca cccggcccac caccacgccg cctcgctgac caccggcggc ggggggacct
     1381 agccccggcc cggctgcacc aggccgacct cataggccag gatcacggcc tggatgcggt
     1441 cgcgcaggcc cagcttggcc agcacgttcc cgacgtgggt cttgacggtg tgctcgctca
     1501 cgaacagcga ggtggccatc tcggcgttgg acagccctct ggccatcagc tcgagggcct
     1561 cccgctcgcg ggcggtcagg gcggccaggg cggccgggtc ggggtcggcc ccggccggcc
     1621 ggcgggcgaa ctcctcgatc aggcggcggg tgacgcgggg ggcgagcagc gcctcgcccc
     1681 ccgcgaccac ccggccggcc gccaccaggt cctcgcgggg caggtccttg agcaggaacc
     1741 cgctggcccc ggcccgcagg gcctcgaaca cgtactcgtc caggtcgaag gtggtcagga
     1801 tcagcacccg gaccgggtcg gccgtccccg ggccggccag gcggcgggtc gcctccagcc
     1861 cgtccaggcg gggcatgcgg atgtccatca ggaccacgtc gggccgcagc cgccgggccg
     1921 cctcgaccgc gtccaggccg tcggcggcct ccccgaccac ctcgatgtcc ggctgggcct
     1981 ccaggatcat ccggaacccg gcccggacca gcccctggtc gtccaccacc aggaccttcg
     2041 ttggcccggt cacggggccg gatccccgat cgggagccgc gcgagcacgg cgaagccgcc
     2101 ggccgccgca gggccggcgt gcagctcgcc gccgaagagc gccgcccgct ccttcatgcc
     2161 cagcaggccg cggccctgcg cgccctggga ccggcccacg ggcggcgacc cgttgctccg
     2221 gccttcgtct gtgacctgga gctccaggtc gtgctccccg taacggacga cgacccgggc
     2281 ccggccggct cccccatgcc gcaacgcgtt ggtcaacgcc tcctggacga tccggtaggc
     2341 ggacaggtcg accccggccg gcagcggcgc ctcggggccc tcgaccacca gctcgacccg
     2401 gagcccggcc cgcccgacct gctcgaccag ggacggcacc tgggccaggc cgggctgggg
     2461 ggcgaggctg ggcgcggcgt cgccgtcccc gtcgccgcgc agcaccccga ggagccggcg
     2521 catctcgacc agggcctggc ggccggtggc ggcgatcg
//
LOCUS       DASSWR010000015         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_876494,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000015 DASSWR010000000
VERSION     DASSWR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876494"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFO47_00230"
     CDS             complement(<1..693)
                     /locus_tag="VFO47_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8787155.1"
                     /translation="MTDRTLITGATGFVGGLLASRLVAEGRQVRALVRHLADRERLLD
                     PRVELAVGHLDDEESLARAADGCQVVYHVAGLNQLCLADPEPLYRVNVEGTRRMLEAA
                     RRAGVRRVVHTSSAATLGGDGSTFLDETAGPPSEFTSHYARSKWEGEQVALGFDGVEV
                     VAVNPSSVQGPGRTTGTAGVFIGYLNGRLPFDLPARFGLCYTLDCVNGHLLAEAKGRP
                     GQRYVLSTATLTN"
     gene            complement(698..1252)
                     /gene="pgsA"
                     /locus_tag="VFO47_00235"
     CDS             complement(698..1252)
                     /gene="pgsA"
                     /locus_tag="VFO47_00235"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET8787156.1"
                     /translation="MGTLTLSTRITLLRIALIPVLLWLLVAEGRVERAAVLAAIVFLV
                     ASFTDFVDGYLARRWQQTTMLGNFLDTTADKLLVVGALLGLMAVDRVNVWVAFVVIAR
                     EIAVLGLRAIAAASNVVISASIWGKIKFNVQVVGITLAILRPEVQLGSLWLDEWAMLA
                     VAVVSAASAVDYFARFGDLIRAGR"
     gene            complement(1290..2102)
                     /locus_tag="VFO47_00240"
     CDS             complement(1290..2102)
                     /locus_tag="VFO47_00240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HET8787157.1"
                     /translation="MSGHASRRAELGQHFLAGGWLAAELVEQAGVGQGDLVVEIGAGT
                     GVLTEALARRAGRVVAVEYDPRLAQRVRTRVAGYPNVRVVTADARTMPPPRRPFRVVA
                     NLPFGSTAAILRRLLGDPRTQLERADLVLQEQAARRYAARRPGTPEMIGWGARYELTI
                     GRRLGPSCFRPPPRVGAAVLVARRRDPPLVPVADRRRFAALVQAAFRHPGLPLRRGLV
                     PPLTYRQLRRLARDLGFPLDARPGDLDAVQWAGLYALLDNGRPGKTGGKPVG"
BASE COUNT          320 a          847 c          772 g          261 t
ORIGIN      
        1 gttggtcagg gtggcggtgc tgagcacgta gcgctgcccg ggccggccct tggcctcggc
       61 cagcaggtga ccgttgacgc agtccagggt gtagcagagc ccgaacctgg ccggcaggtc
      121 gaacggcagc cggccgttga ggtagccgat gaacaccccg gccgtgcccg tggtccgccc
      181 tggcccctgc accgagctgg ggttgacggc caccacctcg acgccgtcga agccgagggc
      241 gacctgctcg ccctcccact tggagcgggc gtagtgggac gtgaactccg acggcggacc
      301 ggccgtctcg tcgaggaacg tgctgccgtc cccgccgagg gtggccgccg agctggtgtg
      361 gaccacccgc cgcaccccgg cccgccgggc cgcctccagc atccgccggg tgccctcgac
      421 gttgacccgg tacaggggct ccgggtcggc caggcagagc tggttcagcc cggccacgtg
      481 gtagacgacc tggcagccgt cggccgccct ggccagcgac tcctcgtcgt ccaggtggcc
      541 gacggccagc tcgacccgcg ggtcgagcag ccgctcacgg tccgccaggt ggcggaccag
      601 ggcccgcacc tggcggccct cggccaccag ccgggacgcc agcagcccac caacgaaccc
      661 ggtcgccccg gtgatcagcg tcctgtcggt catccgctca tcgtcccgcc cgtatcaggt
      721 cgccaaagcg cgcgaagtag tcgacggccg aggcggcgct gacaacggcc accgccagca
      781 tggcccattc gtccagccag agcgaaccca gctggacctc cggccgcagg atggccaggg
      841 tgatcccgac gacctggacg ttgaacttga tcttgcccca gatgctggcc gagatgacca
      901 cgttggaggc ggcggcgatg gcccgcaggc cgagcacggc gatctcccgg gcgatcacca
      961 cgaacgccac ccagacgttg acccggtcca ccgccatcag gcccagcagg gcccccacga
     1021 ccagcagctt gtcggcggtg gtgtcgagga agttgccgag catggtggtc tgctgccagc
     1081 gccgggccag gtagccgtcg acgaagtcgg tgaacgaggc gaccaggaac acgatcgcgg
     1141 ccagcaccgc cgcccgctcc acccgtccct cggcgaccag cagccacagc agcaccggga
     1201 tgagcgcgat gcgcaacagg gtgatgcggg tggagagcgt cagcgtcccc atcgatccct
     1261 cggcagcggt cgggaggtcc ggttcccccc tatcctacgg gcttgccccc ggtcttccca
     1321 ggtcgaccgt tgtcgagcag cgcgtacagg ccggcccatt ggaccgcgtc caggtcgccc
     1381 gggcgggcgt cgagcgggaa cccaaggtcg cgggccaggc ggcgcagctg ccggtaggtc
     1441 aagggcggca ccagcccgcg gcgcagcggc agcccgggat ggcggaaggc cgcctggacg
     1501 agcgccgcga agcgcctgcg gtcggccacc gggaccagcg gcgggtcgcg gcgccgggcg
     1561 accagcacgg ccgccccgac ccgcggcggc ggccggaagc aggaaggccc gagccgacgg
     1621 ccgatggtca gctcatagcg ggccccccag ccgatcatct ccggggtgcc gggtcgcctg
     1681 gccgcgtacc gcctggccgc ctgctcctgg agcaccaggt cggcccgctc cagctgggtc
     1741 cgcgggtcac cgagcagccg ccgcaggatc gccgccgtcg acccgaacgg caggttggcc
     1801 accacccgga acggccgccg cggcggcggc atggtgcgcg cgtcggccgt caccacccgc
     1861 acgttggggt agccggccac ccgcgtccgc acccgctggg ccagccgcgg gtcgtactcg
     1921 accgccacca cccgcccggc ccgccgggcc agcgcctcgg tgagcacccc ggtcccggcc
     1981 ccgatctcga ccaccaggtc gccctgaccg accccggcct gctcgaccag ctcagccgcc
     2041 agccaccctc cggccaggaa gtgctgcccg agctcggcgc gcctcgacgc gtgcccggac
     2101 acggaagacc accatgctca tgcccccagc caacctcgcc caggccgttc cggcaagcga
     2161 attgttcgtg gtgagtcggg tggggttcgg gttcgtgccc
//
LOCUS       DASSWR010000016         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1187265, whole genome shotgun sequence.
ACCESSION   DASSWR010000016 DASSWR010000000
VERSION     DASSWR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187265"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFO47_00245"
     CDS             complement(<1..468)
                     /locus_tag="VFO47_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787158.1"
                     /translation="MSGPPPQAGPPRGREGAPVPTWRLVAGAAVGSLVVLLLPVRPPL
                     GLWVVNGVLLVAAVVDWALAVRPRDLEVEREPPGIVPLGSEARVTWRIAHRAGPSRDR
                     LGLAGRGVRVRLADELAPSLGAVTRRARVVVPARGRAMAATTIRPGRRGRFTPA"
     gene            complement(482..1453)
                     /locus_tag="VFO47_00250"
     CDS             complement(482..1453)
                     /locus_tag="VFO47_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8787159.1"
                     /translation="MSVSTTQPQAPRAAVLRVRDEIAKAVVGQDGAVSGLVAALLVRG
                     HVLLEGVPGVAKTLLVKTLARTLDLDFKRVQFTPDLMPSDVTGQVIFEQRDGSFRFRQ
                     GPVFTNLLLADEINRTPPKTQASLLEAMEERQVSVEGQGHPLPEPFLVVATQNPVEYE
                     GTYPLPEAQLDRFLFKLHVGYPSAEQEQAVLARHDAGMDPHDLATLGVTAVAGRQDLE
                     AARKEVTAVPVEPRVQAYIVAIARATRESPSLELGVSPRGAAMLLHAAKAWAWLAGKQ
                     YVTPDEVKAVAKPTLRHRLELRPEVELEGVTADGVLDGILAAVPVPR"
     gene            complement(1443..2576)
                     /locus_tag="VFO47_00255"
     CDS             complement(1443..2576)
                     /locus_tag="VFO47_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET8787160.1"
                     /translation="MSGRAGARTGVGRALPWVAVVLGVALVVAVAGRGPEEGEPLDPG
                     SPGPLGTKALMDVLRELGGEVGISSDPPGSAVQTTLLLSDDLTPDRRQDLLEWVRQGG
                     TLVVADPSSGVTEVEPAGSTRIGLLDAEIERRCDVAALGNVGRVAAPGGVVFEVPAGR
                     GAAAGTRACFPRNDGAWLLVQPLGLGTVVRLGGASVLVNEELGEADNGLLLASLLVPA
                     PGTAVRVLRPPLPGGGNAGLGDLVAPRVRLALWQLVVAFVLLALWRARRLGRPVAEPQ
                     PVQLPGAELVMAVGNLLQRAKGRGQAARLLTDDLRRSLAERLGLPPSAPPDLVADTAA
                     ARTGIPRERVLRALSPTTPRDEAGLVALSQAVDTVRREVTRVR"
     gene            complement(2573..3172)
                     /locus_tag="VFO47_00260"
     CDS             complement(2573..3172)
                     /locus_tag="VFO47_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET8787161.1"
                     /translation="MPVQDPDQVREVVREVLSRPEFRPAERSLVERIYDWVLEAIGRL
                     LAALGGSGAGGIIGLILLALVLVGVGVLVARFSRGLTPSPEVAAAVPGIRRRSAAEWR
                     AEAEAQERAGAWREAVRSRYRALVADLASRGLVEEVPGRTAGEYRREVGRALPDAAGD
                     FAGATELFEVAWYGRSPTGAQEATHLRDLSDRVLQRAGS"
     gene            complement(3264..4076)
                     /locus_tag="VFO47_00265"
     CDS             complement(3264..4076)
                     /locus_tag="VFO47_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787162.1"
                     /translation="MMFSDLLDGSFRLLKANLSAIVLVAAVFVIPVNLVAAFFQRDLL
                     GGYGFLELIQDPSLAEQAAGTGPSDGAWIGIVIAALAGFLVTPFIGGATSMIVAASYL
                     GGELKAGPAMRATGRAFLVLLAVFLMTALLKLAGLVLCLVGALIPMTFFLVTTPVVMV
                     EHAGPIQAMARSVTLVRPRFWPVMAIGIGSGLIASFLGNILATPFSIAALAVGYRWGW
                     ILAAIGAIIPALITTPFVSILATLVYFDLRIRNEGFDLQMIAAELGRGAPAR"
     gene            4426..>4850
                     /locus_tag="VFO47_00270"
     CDS             4426..>4850
                     /locus_tag="VFO47_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8787163.1"
                     /translation="MKGRVLVVDDDPSLGEMLGIVLRQEGFEPVFVRSGDQAIAAFHQ
                     AKPDLVLLDLMLPGKDGLEICRELRLESGVPIVMLTAKTDTVDVVLGLESGADDYITK
                     PFSAKELVARIRARLRRRDTDGAETLRFHDIEMDVMAHQV"
BASE COUNT          650 a         1868 c         1806 g          526 t
ORIGIN      
        1 ggccggggtg aaccgccccc gccggcccgg ccggatcgtg gtcgccgcca tcgcccgccc
       61 ccgggccggc accaccaccc gcgcccgccg ggtcaccgcc cccagcgacg gcgccagctc
      121 gtccgccagc cgcacccgca ccccccggcc ggccaggccc aagcggtccc gggatggccc
      181 ggcccggtgg gcgatgcgcc aggtgacccg ggcctcgctg ccgaggggca cgatcccggg
      241 cggctcccgc tcgacctcga ggtcgcgggg ccgcaccgcc agcgcccagt cgacgaccgc
      301 ggccaccagc agcaccccgt tcaccaccca cagccccagc ggcggccgga ccggcagcag
      361 cagcaccacc agcgacccca ccgccgcccc cgccaccagc cgccaggtcg gcaccggcgc
      421 gccctcccgt cccctggggg gcccggcctg ggggggcggc ccgctcaccc ggcctccccg
      481 gtcaccgggg gaccgggacg gcggccagga tgccgtcgag gacgccgtcg gcggtcacgc
      541 cctcgagctc gacctccggc cgcagctcga gccggtgccg cagggtcggc ttggccaccg
      601 ccttgacctc gtcgggggtg acgtactgct tgccggccag ccaggcccag gccttggcgg
      661 cgtgcagcag catggccgcc cccctggggg agacgccgag ctccagcgac ggcgactccc
      721 gggtcgcccg ggcgatggcc acgatgtagg cctggacccg cggctcgacc gggacggccg
      781 tcacctcctt gcgggccgcc tccaggtcct ggcggccggc caccgccgtc acccccaggg
      841 tggccaggtc gtgggggtcc atgccggcgt cgtgacgggc cagcacggcc tgctcctgct
      901 cggccgaggg gtagccgacg tgcagcttga acaggaaccg gtcgagctgg gcctcgggca
      961 ggggataggt gccctcgtac tcgaccgggt tctgggtggc gacgaccagg aacggctcgg
     1021 gcagcgggtg cccctggccc tccaccgaca cctggcgctc ctccatcgcc tccagcagcg
     1081 aggcctgggt cttgggcggg gtgcggttga tctcgtcggc cagcagcagg ttggtgaaca
     1141 ccggcccctg gcggaagcgg aacgagccgt cgcgctgctc gaagatcacc tgaccggtca
     1201 cgtccgacgg catcaggtcg ggggtgaact ggacccgctt gaagtccagg tcgagggtcc
     1261 gggcgagggt cttgaccagc agcgtcttgg ccaccccggg gacgccctcc agcagcacgt
     1321 ggccgcggac gagcagggcc gcgaccagcc ccgacaccgc cccatcctgg ccgacgaccg
     1381 ccttggcgat ctcgtcgcgg acccggagca ccgccgcccg gggcgcctgc ggctgcgttg
     1441 tgctaacgga cacgggtgac ctcccggcgg acggtgtcga cggcttggct gagggcgacg
     1501 agcccggcct cgtcgcgggg cgtggtcggg ctgagggccc ggagcacccg ctcgcgcggg
     1561 atcccggtcc gggcggcggc cgtgtcggcc accaggtcgg gtggggccga cgggggcagg
     1621 ccgagccgct cggccagcga gcggcgcagg tcgtcggtga gcaggcgcgc cgcctggccg
     1681 cgccccttgg cccgctggag caggttgccg acggccatga ccagctcggc cccggggagc
     1741 tggaccggct gcggctcggc cacgggccgg cccagccggc gcgcccgcca gagggcgagc
     1801 aggacgaagg cgacgacgag ctgccacagc gccagccgca ccctgggggc gaccaggtcg
     1861 cccagcccgg cgttccctcc gccgggcagg ggcggccgca gcacccggac ggccgtcccc
     1921 ggcgccggga ccagcaggct ggccagcagc aggccgttgt cggcctcgcc gagctcctcg
     1981 ttgacgagca cgctggctcc gccgaggcgg acgacggtgc cgaggccgag cggctggacc
     2041 agcagccagg cgccgtcgtt gcgggggaag caggcccggg tgccggccgc cgccccccgg
     2101 cccgcgggga cctcgaacac gaccccgccg ggcgccgcca cccggcccac gttgcccagg
     2161 gcggccacgt cgcagcgccg ttcgatctcg gcgtcgagca ggccgatgcg ggtcgagccg
     2221 gccggctcga cctcggtcac gccggagctg gggtcggcca ccaccagggt cccgccctgc
     2281 cggacccact cgagcaggtc ctggcgccgg tcgggggtga ggtcgtcgct gagcagcagg
     2341 gtcgtctgga cggcgctccc gggcgggtcg gaggagatgc ccacctcgcc gcccagctcc
     2401 cgcagcacgt ccatcagcgc cttggtcccc agcggccccg gcgaccccgg gtccagcggc
     2461 tcgccctcct cgggcccccg ccccgcgacc gccaccacca gcgccacccc cagcacgacc
     2521 gccacccacg gcagcgccct cccgaccccg gtccgggccc cggcccgccc gctcacgagc
     2581 cggcccgctg gaggacgcgg tcggacaggt cgcggaggtg ggttgcctcc tgggcgccgg
     2641 tgggggagcg gccgtaccag gcgacctcga acagctcggt ggcgccggcg aagtcgccgg
     2701 cggcgtcggg cagggcgcgg ccgacctcgc gccggtactc gccggcggtg cggccgggca
     2761 cctcctcgac caggccgcgg gaggcgaggt cggccaccag ggcccggtag cgggagcgga
     2821 ccgcctcccg ccaggcgccg gcccgctcct gcgcctccgc ctcggcgcgc cactcggccg
     2881 ccgaccgccg ccggatcccc gggacggccg ccgcgacctc ggggctgggc gtcagccccc
     2941 gcgagaaccg ggccaccagc accccgactc cgacgaggac cagcgccagc aggatcagcc
     3001 cgatgatccc gccggccccg cttccgccga gcgccgccag gaggcgcccg atcgcctcca
     3061 gcacccagtc gtagatccgc tccaccagcg accgttcggc cggccggaac tccggcctcg
     3121 acaggacctc gcggacgacc tcccgcacct ggtccgggtc ctgcaccggc acccgcgggt
     3181 ccccctgccg ctgccccagc cccaccacgg cgggcaggaa cagcccggcg gcgggacgga
     3241 gggccgggga gcggggcggt cggtcagcgg gccggggcac cgcggcccag ttcggcggcg
     3301 atcatctgca ggtcgaagcc ttcgttcctg atccggaggt cgaagtagac cagggtggcc
     3361 aggatcgaca cgaacggcgt cgtgatcagg gccgggatga tggccccgat ggcggccagg
     3421 atccagcccc agcggtagcc gacggccagc gccgcgatcg agaacggggt ggccaggatg
     3481 ttcccgagga agctggcgat gaggccgctc ccgatcccga tggccatgac cggccagaac
     3541 cgcgggcgga ccagggtcac ggaccgggcc atggcctgga tgggcccggc gtgctcgacc
     3601 atcaccaccg gcgtggtcac caggaagaag gtcatgggga tgagcgcccc gaccaggcac
     3661 aggaccagcc cggccagctt gagcagcgcg gtcatgagga acacggccag caggacgagg
     3721 aaggcgcgtc cggtggcccg catggccggg ccggccttga gctccccgcc gaggtagctg
     3781 gcggcgacga tcatggaggt ggcgccgccg atgaacgggg tgaccaggaa gccggcgagg
     3841 gcggcgatca cgatgccgat ccaggccccg tcggagggcc cggtccccgc cgcctgctcg
     3901 gccagcgacg ggtcctggat gagctcgagg aagccgtagc cgccgagcag gtcgcgctgg
     3961 aagaacgcgg ccaccaggtt gaccgggatg acgaacacgg ccgcgaccag gacgatggcg
     4021 gagaggttgg ccttgagcag gcggaacgac ccgtcgagga ggtcgctgaa catcatcggg
     4081 tgcagcggca gcgggcccgt ccgggccgac ccggccttcg gcaacggcgg gtacggcgac
     4141 ggcacccccc accccggcgc cgcacccggc ggcggccccc accccggcgg gggcgtggcg
     4201 ggcgggccgg gcagctggcc cggcgggtcc cagggtggtg gtggtgggct ggtcacggcc
     4261 ctcccctctt catcggcgct cgcgcgcatt gtcacgaggc cgcacgggcc ggtcaacggc
     4321 gtcagccgga cgtctgtgga ttgtggaaat ggttgatact ccactgttgg acgccgacgt
     4381 gacatccttg gccgattgtc ggcgccggga agggggcggt aacgcgtgaa gggcagggta
     4441 ctggtcgtcg acgacgaccc gtcgctcggc gagatgctcg ggatcgtcct gcgccaggag
     4501 ggcttcgagc cggtgttcgt ccgctcgggc gaccaggcca tcgccgcctt ccaccaggcc
     4561 aagcccgacc tggtcctgct cgatctcatg ctgcccggca aggacggcct ggagatctgc
     4621 cgcgagctgc ggctggagtc cggcgtgccc atcgtgatgc tgaccgccaa gaccgacacc
     4681 gtcgacgtcg tcctcgggct cgagtccggg gccgacgact acatcaccaa gccgttctcg
     4741 gccaaggagc tggtggcccg catccgggcc cggctgcgcc gccgcgacac cgacggcgcc
     4801 gagaccctcc gcttccacga catcgagatg gacgtcatgg cccaccaggt
//
LOCUS       DASSWR010000017         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_170285,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000017 DASSWR010000000
VERSION     DASSWR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170285"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="VFO47_00275"
     CDS             complement(<1..2094)
                     /locus_tag="VFO47_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8787164.1"
                     /translation="MTVPRRIRVGVDTGGTFTDVVAFDEDSGELVATKTPSTPADPAE
                     GFMDGIAKVLATLGVGPEAVTAVCHGTTVATNQLLEGRVGRLGFVTTEGFGALLEIAR
                     QSVPDGYGNSYFWVKPPRIVPADLVRTVGGRLDHSGAELRPFDEEGARAAARFLRDRA
                     VTAVGVCFLHAYANPAHERRMREVLAAEHPGAAVSLSSEVLREYREYERAMTTLVDAA
                     VKPRVGRYVATIARHLEELVPGTPPPFYVMKSNGGVLSAAEVVHQPISTVLSGPAAGA
                     LGAALATRTAGFDRVLTLDGGGTSTDVSVVLDGEPALTTEGSVGGYPSKIPMVDVVTV
                     GAGGGSVAWLSREGGLKVGPRSAGADPGPMCYGRGGAEPTVTDASLVLGRIPPHLLGG
                     EVPLDPAAAATGLEALGAKLGLELPAVAAGVLEISAWNQANALRQVTVQRGLDVRDFV
                     LATFGGSGSLAVCRLLELLGLPAAVVPRDPGNLSAFGLLTVDVKNDEVQTAVAGHADL
                     DPAAVAATLAALQERAARALDAEGFPRPAHRYLRSADLRYVGQAFEVRVPVPDGPVDH
                     ALTDATVAAFHDAHQRRYGYSFRDDPTQQVEWVNLRVTGVGPIRRPTLRPLTPDTPRP
                     PGASPSTPASIPVPAGTRPACFDPARGFVDTAIYHREDLSPGDLVGGPAVVEEYGATV
                     PLHPGFRARIDRFGNL"
     gene            complement(2091..3002)
                     /locus_tag="VFO47_00280"
     CDS             complement(2091..3002)
                     /locus_tag="VFO47_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET8787165.1"
                     /translation="MRAFTAAAVQTAPAPGPLTAASVKANLDTCVDHVERCVAATAAD
                     LVVLPESATTGYTPGVDPATLWDLVSEVPGPVTEPLQDAARRLGVHLVAGTYERGRER
                     GTVHNTAVLVGPTGDVLGSYRKTHVFYLEDRDAGGWVTRGDQAVVVDTDLARIGLLIC
                     YDGDFPELARAEAVLGAEVLCRPSALLRPVDIWELTNRARAYDNHVFVVGANATGADP
                     AGTLYFGNSMVVSPVAEVMARAATQPGWCCARLDPATAMATVAPGASIPQRFDHLADR
                     NLALVERHLDDLRRPARTRFPHPPPER"
BASE COUNT          369 a         1029 c         1274 g          365 t
ORIGIN      
        1 caggttgccg aagcggtcga tccgggctcg gaaccccggg tggagcggca ccgtggcgcc
       61 gtactcctcc accaccgccg gcccgcccac gaggtcccct ggcgacaggt cctcacggtg
      121 atagatcgcc gtgtcgacaa atccacgcgc cgggtcgaag caggcgggcc gggtcccggc
      181 gggaacgggg atggaggctg gagtggaggg ggaggcgccc ggggggcggg gggtgtctgg
      241 ggtgaggggg cggagggtgg ggcggcggat ggggccgacg ccggtgaccc ggaggttgac
      301 ccactcgacc tgctgggtgg ggtcgtcgcg gaagctgtag ccgtagcggc gctggtgggc
      361 gtcgtggaag gcggccacgg tggcgtcggt gagggcgtgg tcgacggggc cgtcggggac
      421 cgggacgcgg acctcgaacg cctggccgac gtagcgcagg tcggccgagc gcaggtagcg
      481 gtgggccggc cgggggaagc cctcggcgtc cagggcccgg gcggcgcgct cctggagggc
      541 ggccagggtg gcggcgacgg cggccgggtc gaggtcggcg tggccggcga ccgccgtctg
      601 gacctcgtcg ttcttgacgt cgacggtgag caggccgaag gcggacaggt tgcccgggtc
      661 gcgggggacg acggccgcgg gcaggccgag cagctccagc agccggcaga cggccagcga
      721 ccccgacccg ccgaaggtgg cgagcacgaa gtcgcgcacg tccaggccgc gctggacggt
      781 gacctggcgc agggcgttgg cctggttcca ggccgagatc tccagcaccc cggcggcgac
      841 ggcgggcagc tcgagcccga gcttggcccc cagcgcctcc aggccggtgg cggccgcggc
      901 cgggtcgagg gggacctcgc cgccgagcag gtggggcggg atccggccca ggacgaggct
      961 ggcgtcggtc acggtcggct cggccccgcc gcgtccgtag cacatcggcc cggggtcggc
     1021 cccggccgag cggggcccga ccttgaggcc gccctcccgc gacagccagg ccaccgaccc
     1081 gccgcccgcg ccgacggtga ccacgtcgac catggggatc ttggacgggt agccgccgac
     1141 gctgccctcg gtggtcaggg ccggctcgcc gtccaggacc acgctgacgt cggtcgaggt
     1201 gccgccgccg tccagggtca ggacccggtc gaacccggcc gtcctggtcg ccagggccgc
     1261 cccaagggcc ccggccgccg gcccggacag cacggtcgag atcggctggt ggacgacctc
     1321 ggcggccgag aggacgccgc cgttggactt catcacgtag aacggcggcg gcgttcccgg
     1381 caccagctcc tccaggtggc gggcgatggt ggccacgtag cggccgaccc gcggcttgac
     1441 ggcggcgtcg accagggtgg tcatggcccg ctcgtactcg cggtactcgc gcagcacctc
     1501 gctggagagc gacaccgccg ccccggggtg ctcggcggcc aggacctcgc gcatgcgccg
     1561 ttcgtgggcc gggttggcgt aggcgtgcag gaagcacacg cccacggcgg tcaccgcccg
     1621 gtcgcgcagg aagcgggcgg ccgcccgggc cccctcctcg tcgaacgggc gcagctcggc
     1681 accgctgtgg tcgagccggc cgccgacggt cctgaccagg tcggcgggga cgatccgggg
     1741 cggcttgacc cagaagtagc tgttgccgta gccgtcgggg acgctctggc gggcgatctc
     1801 cagcagggcc ccgaagccct cggtggtgac gaagccgagc cggccgaccc ggccttcgag
     1861 gagctggttg gtggccacgg tggtgccgtg gcagacggcg gtgaccgcct ccggcccgac
     1921 gcccagggtg gccaggacct tggcgatccc gtccatgaac ccctcggccg ggtcggccgg
     1981 ggtcgagggg gtcttggtcg ccaccagctc gccgctgtcc tcgtcgaagg cgaccacgtc
     2041 ggtgaaggtc ccgccggtgt cgaccccgac ccggatccgg cgcgggacgg tcatcgttcg
     2101 ggcggcgggt gcgggaacct cgtcctggcg gggcggcgga ggtcgtcgag gtggcgctcg
     2161 accagggcca ggttgcggtc ggcgaggtgg tcgaagcgct gggggatgct ggccccgggg
     2221 gcgacggtgg ccatggccgt ggccgggtcg aggcgggcgc agcaccagcc cggctgggtg
     2281 gcggccctgg ccatcacctc ggcgaccggg gagacgacca tcgagttgcc gaagtacagc
     2341 gtcccggccg ggtcggcgcc ggtggcgttg gccccgacca cgaacacgtg gttgtcgtag
     2401 gcccgggccc ggttggtcag ctcccagatg tcgaccgggc gcagcagcgc cgacggccgg
     2461 cacaggacct cggccccgag cacggcctcg gccctggcca gctccgggaa gtcgccgtcg
     2521 tagcagatga gcaggccgat ccgggccagg tcggtgtcga cgaccacggc ctggtcgccg
     2581 cgggtgaccc agccgccggc gtcgcggtcc tccaggtaga agacgtgggt cttgcggtag
     2641 ctgccgagga cgtcgccggt cgggccgacc aggacggcgg tgttgtggac ggtgccgcgc
     2701 tcgcggccgc gttcgtaggt gccggcgacg aggtggacgc cgaggcgccg ggccgcgtcc
     2761 tggaggggct cggtgaccgg gccggggacc tcgctgacca ggtcccagag ggtggccggg
     2821 tcgaccccgg gggtgtagcc ggtggtggcc gactcgggga gcacgaccag gtcggcggcg
     2881 gtggcggcga cgcagcgctc gacgtggtcg acgcaggtgt cgaggttggc cttgaccgag
     2941 gcggcggtca gggggcctgg ggccggggcg gtctggacgg ccgcggcggt gaaggcccgc
     3001 atggcggctc agcgcctccg ggcccggcgg gccaggt
//
LOCUS       DASSWR010000018         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_678766,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000018 DASSWR010000000
VERSION     DASSWR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678766"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFO47_00285"
     CDS             <1..310
                     /locus_tag="VFO47_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET8787166.1"
                     /translation="AFDPAAGRWSAVADLPTARGGLAATATAAGEVVAVGGEAAATFP
                     EAEAFDPASGRWRSLPPLPTPRHGLGVVAVGDVVYVLAGGPQPGLHTSAANEAIDLGG
                     "
     gene            337..549
                     /locus_tag="VFO47_00290"
     CDS             337..549
                     /locus_tag="VFO47_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8787167.1"
                     /translation="MSLISDPMFVCHCRALTDGEISEVITGGACDLDEVGDRCGAGIT
                     CGGCCPLIEELLAQHAEAGRPAAVAS"
     gene            complement(546..1472)
                     /locus_tag="VFO47_00295"
     CDS             complement(546..1472)
                     /locus_tag="VFO47_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018730800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET8787168.1"
                     /translation="MGGTRMLDGGGAGPPVFTYQVVPGEPSVGVLRILGRELPPGGPP
                     PGHAHSHDFLVLAYFERGGGSLRLAGREWPVEAGDAYVVAPGEVIAVGGQAAGLRGAE
                     GWAVFFPPEVLGGQAPGAFLSWRAHPLLYPFVRGTSGSAQRLRVAPAQRAAWSERFAA
                     LDTELRGRHDGYQEAALAHLTLLLVELSRLAVDVAGDLRLKDEPLLAGVFGFIEERFR
                     ERISLKDVASAVSLTPGHLTTVVRRRTGRTVQDWIAERRMAEARRLLVQTDLTVEEVS
                     RRVGYGDAGYFARHFRRAHGATPLGWRRAGRP"
     gene            1632..2300
                     /locus_tag="VFO47_00300"
     CDS             1632..2300
                     /locus_tag="VFO47_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8787169.1"
                     /translation="MDVTTEGRGDHYLPGMGHDRLLPLYDPVCRLLRISRFHRPLVDQ
                     AGIRPGQRVLEIGCGTGNLALLVRRLQPGAEVVGLDPDPRALARARRKAERRSLPVRF
                     DRGFAEKLPYADASFDRVLSALMFHHLGPDEQERALREVRRVLEPGGSLHLVDFGGAR
                     ERSDGLMARLGHRSPRLEDNFGGRIPALMREAGLTDPSEVAHRVSRVMGRLTYYRAVA
                     PAAA"
     gene            complement(2297..2767)
                     /gene="bfr"
                     /locus_tag="VFO47_00305"
     CDS             complement(2297..2767)
                     /gene="bfr"
                     /locus_tag="VFO47_00305"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET8787170.1"
                     /translation="MQGDPRVLELLNQHLTFELTAINQYFLNSRMCANWGYDRLAKRH
                     REIAMAEMRDTEELIDRILYLEGHPNLQRLGTVKVGETAVEQIRLAVELERTAVRNLD
                     GYVATCAEIGDQGTREFLAAMIAEEERHVDWWETQLDTIAQIGEGLYLAQQVRD"
     gene            complement(2817..>3362)
                     /locus_tag="VFO47_00310"
     CDS             complement(2817..>3362)
                     /locus_tag="VFO47_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8787171.1"
                     /translation="PRRAPGEAVDALEEAIALLRLLWSDQRSVRFTGQHYRVVGVKPG
                     PAPAHPIGIWIGGFGPRMLALTGRLADGWVPSSPYVPPERLPAAQARIDEAAAAAGRG
                     PAALRRLYNISGRIGPGGGGFLDGPAAQWVEQLLPLVTETGMDTFVLWPSESPARQLE
                     LFAAEVAPALREAVAAHRGRG"
BASE COUNT          450 a         1261 c         1222 g          429 t
ORIGIN      
        1 ggcgttcgac ccggccgccg gccgctggag cgcggtcgcc gacctgccga cggcccgggg
       61 cggcctggcc gccaccgcca ccgccgccgg ggaggtggtg gccgtcggcg gcgaggccgc
      121 ggccaccttc ccggaggcgg aggcgttcga cccggccagc ggccgctggc gctccctgcc
      181 gccgctgccc accccccgcc acgggctcgg cgtggtcgcc gtcggcgacg tggtctacgt
      241 cctcgccggc ggtccccagc ccggcctgca cacctcggcc gccaacgagg ccatcgacct
      301 cggcgggtag cggatccccc ttgtgcccgg gattaggtga gcctaatatc ggacccgatg
      361 ttcgtctgcc actgccgggc cctcaccgac ggagagatct ccgaggtgat cacgggcgga
      421 gcctgcgacc tcgacgaggt cggggaccgc tgcggcgccg gcatcacctg cggcggctgc
      481 tgccccctga tcgaggagct gctggcccag cacgccgagg ccgggcggcc ggcggcggtg
      541 gcgtcctagg ggcggccggc gcggcgccag ccgagcgggg tggcgccgtg ggcgcgacgg
      601 aaatgcctgg cgaagtagcc ggcatccccg tagccgaccc tccggctcac ctcctccacg
      661 gtcaggtcgg tctggaccag cagcctgcgc gcctcggcca tgcgccgctc ggcgatccag
      721 tcctgcacgg tgcgacccgt ccggcgtcgc accaccgtgg tgagatggcc cggcgtcagg
      781 ctcaccgcgc tggccacgtc cttgagcgag atccgctccc tgaagcgttc ctcgatgaag
      841 ccgaacacgc cggcgagcag gggctcgtcc ttgagccgga ggtccccggc gacgtccacg
      901 gccagccgcg acagctccac cagcagcagc gtcaggtgcg cgagggccgc ctcctggtag
      961 ccgtcgtggc ggccgcgcag ctcggtgtcg agggccgcga agcgctcgga ccacgccgcc
     1021 cgctgggcgg gggccacccg caggcgctgg gcgctcccgg aagtcccccg gacgaacggg
     1081 tacagcagcg ggtgggcgcg ccaggagagg aaggcgccgg gtgcctggcc accaaggacc
     1141 tcgggcggga agaacaccgc ccagccctcg gccccgcgga ggccagcggc ctgcccgccg
     1201 acggcgatca cctcgccggg ggcgacgacg taggcgtcgc cggcctccac cggccactcc
     1261 cggccggcca gccgcaacga cccgccgccc cgttcgaagt aggcgagcac cagaaagtcg
     1321 tgggagtggg cgtgaccggg cggtggccca cccggcggca gctcccggcc gaggatccgc
     1381 aggaccccca cggagggctc gccgggcacc acctggtagg tgaagaccgg cggtcccgcc
     1441 ccgccgccgt ccagcatcct cgtcccaccc acgccggtcc ctcccctccc accgccgcac
     1501 cccgcggccg ggcccccaga gcccaaagat catctcagga caaccgaaca tcctgtcttt
     1561 tccccgcata caccgaccga aatactggtc ccatggcaaa acatcgtact aggcaggagc
     1621 ggaggacggg catggacgtc acgacggaag gtcgcggaga ccactacctc cccggcatgg
     1681 gacacgaccg gctgctgccg ctctacgacc ccgtctgccg gttgctgcgc atctcccggt
     1741 tccatcggcc actggtggac caggcaggca ttcggccggg ccagagggtc ctggagatcg
     1801 ggtgcggcac cgggaacctg gcactgctgg tccggcggct ccagccgggc gcggaggtcg
     1861 tcgggctcga ccccgacccc agggcgctcg cccgcgcacg ccgcaaggcc gagcgacggt
     1921 cgcttccggt gcggttcgac cgcgggttcg cggagaagct cccgtacgcc gacgcgtcgt
     1981 tcgaccgggt gctgtccgcc ctcatgttcc accatctagg accggacgag caggagcgag
     2041 cgctgcgcga ggtgcggcgc gtgctcgaac ctggcggctc cctgcatctt gtcgacttcg
     2101 ggggagccag ggagcgctcc gacgggctca tggcccgcct gggtcaccga agcccgcggc
     2161 tggaggacaa cttcgggggc cgtatccccg cgctcatgcg cgaagccggc ctcaccgacc
     2221 cctccgaggt ggcccacagg gtctcgcggg tcatgggacg cctcacctac taccgcgccg
     2281 tggcgccggc cgccgcctag tctcggacct gctgggccag gtacaggccc tcgccgatct
     2341 gggcgatggt gtccagctgg gtctcccacc agtcgacgtg ccgctcctcc tcggcgatca
     2401 tcgcggccag gaactcgcgg gtgccctggt cgccgatctc ggcgcaggtg gccacgtagc
     2461 cgtccaggtt gcggaccgcg gtccgctcca gctcgacggc cagccggatc tgctcgaccg
     2521 ccgtctcgcc caccttgacc gtgcccagcc gctgcaggtt ggggtggccc tccaggtaga
     2581 ggatgcggtc gatcagctcc tcggtgtcgc gcatctccgc catggcgatc tcgcggtggc
     2641 gcttggccag gcggtcgtag ccccagttgg cgcacatcct ggagttgagg aagtactggt
     2701 tgatcgcggt cagctcgaag gtcaggtgct ggttgagcag ctcgaggacg cgcgggtcac
     2761 cctgcaaggg ggccctcctt gggtcggcga cggaacggtg aggtgggtag tctccctcaa
     2821 ccgcggccgc ggtgggcggc cacggcctcg cgcagggccg gggcgacctc ggccgcgaac
     2881 agctccagct ggcgggccgg ggactccgac ggccagagca cgaaggtgtc catgccggtc
     2941 tcggtgacca gcgggaggag ctgctcgacc cactgggcgg ccgggccgtc caggaacccg
     3001 ccgccgccgg ggccgatccg gccggagatg ttgtagaggc ggcggagggc cgccggtccg
     3061 cggccggcgg cggcggccgc ctcgtcgatg cgggcctggg cggccgggag ccgttccggg
     3121 ggcacgtacg gtgaggacgg cacccagccg tcggccagcc ggccggtcag ggccagcatc
     3181 cgtgggccga agcccccgat ccagatgccg atgggatggg ccggcgccgg ccccggcttg
     3241 accccgacga cccggtagtg ctggcccgtg aaccgcaccg accgctggtc actccagagc
     3301 agccgcagca gggcgatggc ctcctcgagc gcatccacgg cctcgcccgg ggcgcggcgg
     3361 gg
//
LOCUS       DASSWR010000019         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1244128, whole genome shotgun sequence.
ACCESSION   DASSWR010000019 DASSWR010000000
VERSION     DASSWR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244128"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFO47_00315"
     CDS             <1..581
                     /locus_tag="VFO47_00315"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET8787172.1"
                     /translation="LVGALVGPASLGIYAVASRYAELLRLPSLAMNYVLYPDYARDGG
                     KVAADKARAMLPGIGWIPAALAVPMALAAPLVLPLFYGPAFKEAVLPACVLLIGLAGG
                     GVIGVISAYLQGVGRPGLYSAAVGAGLPVTVALDLLLIPHFGVMGAAVASTAAYVTTT
                     GVLVACFRVVTRAEGRAARAAGAVESKAEVPR"
     gene            578..1279
                     /locus_tag="VFO47_00320"
     CDS             578..1279
                     /locus_tag="VFO47_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET8787173.1"
                     /translation="MRVLTVAAHPDDETLGAGGTMARLAALGHEVWICILTDGVTARH
                     AHVEQQKECAIGAADVLGAANVVFCELPDQRLDSLPLLDVITPIEKCIGELRPEIVFT
                     HFKEDVNQDHRTVFQATMVAARPVEGTSVRQLLCYETASSTEWAPPFPGSVFSPNVFV
                     DISSTLPRKLEAMRSYERTWAGEMRPYPHPRSYEAIEAYARRHGVAAGVAAAEPFMLV
                     RQVHHEGGLSSDHRF"
     gene            1263..1886
                     /locus_tag="VFO47_00325"
     CDS             1263..1886
                     /locus_tag="VFO47_00325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET8787174.1"
                     /translation="MITGSDIAPGKRLFDVAVSLTALVLLVPVLVVIALLVKFGSPGP
                     VLYRQQRVSRGGRLFELLKFRTMVVGADRMSANVSATGDPRVTPVGAFLRKSYLDELP
                     QLLNVLRGDMSLVGPRPETPEFVALYNPEERRVLTVRPGLAGPSTLAFMDEADLLAAA
                     PDPVAFYTTTVLHDRVRADLAYLERRSIGYDIRLLATQVLSIVRRLR"
     gene            1883..2926
                     /locus_tag="VFO47_00330"
     CDS             1883..2926
                     /locus_tag="VFO47_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HET8787175.1"
                     /translation="MTTMPPAPPVQVERWTNGAAWDQFVGGARDANIGHRWAWLGIVS
                     EVYRHRTFPLAAIRDGVLAGVLPLVLVRSRLFGRHLVSMPFLDSGGLCTDGDVASEQA
                     LVATAVELAGDHGAKLELRHLTERRIGLPASLEKVTMTLDLTGGEDAVWRSIRSTRRG
                     QVRKARRNGLEASWHGQEAVGQFYRVWAANMRDLGSPAHRRAYFARIAASLPGDARFV
                     LVRDGAETVGAGLALVHGDRIVLPWVSSLRSSFSRGPNQLLYWEAFRYGIERGCTVFD
                     FGRSSWNSGTFEAKRQYGAEPEQLYWHRFPAEEGTGGDGADKLEWATRVWKRLPVPVA
                     NTAGALVRGGLPN"
     gene            3002..3841
                     /locus_tag="VFO47_00335"
     CDS             3002..3841
                     /locus_tag="VFO47_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787176.1"
                     /translation="MPPGGPAGEAIASALVADVAGLPLPVVLAAGGVVLLIGVFTARA
                     LRARVSKVDDVEGSTGLVVIGEVPARSLGRPMPSMNGLPLRPVDGLQEICRMLEHNGL
                     GTAITVLTIVRAGIRGPEASFASDLTQTLAAQGKNVLLVQANLRQPSGQTAMGLAQAE
                     GLADLLEDEDLDPVTLLVSVAQRLLVLPSGTPRSDPAALLARPRLKQVIGTLRDLGFI
                     AIIDAPPAAFARDVLSLAREGDATLLIVRTGSRWRDVEAAARMLRYGDVSDPAAVVVG
                     SRW"
     gene            3838..4182
                     /locus_tag="VFO47_00340"
     CDS             3838..4182
                     /locus_tag="VFO47_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787177.1"
                     /translation="MRRRRAWLLAAVAAGAVLVAAVAGIRSRALQGSPEPGTTRYVSV
                     DGSDGNPGSLSRPWRTIAFAAGQLRPGDTLLIRGGTYLELPTVTASGTAAAPITIQSY
                     PGESAVIDSGPA"
BASE COUNT          520 a         1596 c         1507 g          602 t
ORIGIN      
        1 ccctcgtcgg ggcgctggtc gggcccgcct cgctcggcat ctacgccgtg gcctcgcggt
       61 acgccgagct gctgcggctg ccctcgctgg ccatgaacta cgtgctctac ccggactacg
      121 cccgcgacgg gggcaaggtg gccgccgaca aggcgcgggc catgctgccc gggatcggct
      181 ggatcccggc cgccctggcc gtccccatgg ccctggccgc gccgctggtc ctgccactgt
      241 tctacggccc ggccttcaag gaggcggtgc tgccggcctg cgtgctgctg atcgggctgg
      301 ccggcggcgg ggtcatcggg gtcatcagcg cctacctgca gggcgttggc cggccgggcc
      361 tgtactcggc ggccgtcggc gccggcctgc cggtgacggt ggcccttgac ctgttgctga
      421 tcccgcactt cggggtgatg ggcgcggcgg tcgcctcgac cgccgcctac gtgacgacga
      481 ctggcgtgct ggtggcgtgc ttccgcgtgg tgacccgcgc ggaaggacgg gcggcgcgtg
      541 cagcgggggc ggtcgagtcc aaggcggagg taccaaggtg agagtcctga ccgtggcggc
      601 acatcctgac gacgagaccc tgggcgccgg ggggaccatg gcccggctgg ccgccctagg
      661 gcacgaggtg tggatctgca tcctcaccga cggcgtcacg gcccggcacg cgcacgtcga
      721 gcagcagaag gagtgcgcca tcggggcggc cgacgtcctc ggggccgcca acgtcgtgtt
      781 ctgcgagctc cccgaccagc ggctggactc gctgcccctg ctcgacgtca tcaccccgat
      841 cgagaagtgc atcggcgagc tgcggcccga gatcgtcttc acgcacttca aggaggacgt
      901 gaaccaggac caccggaccg tcttccaggc gacgatggtg gccgcacggc ccgtcgaggg
      961 gacgagcgtg cggcagctgc tctgctacga gaccgcctcg tccaccgagt gggcgccgcc
     1021 gttcccgggc agcgtcttca gtcccaacgt gttcgtcgac atcagcagca cgctgccgcg
     1081 caagctggag gcgatgcgct cctacgagcg cacctgggcc ggcgagatgc ggccataccc
     1141 gcacccgcgt tcctacgagg cgatcgaggc ctacgcccgg cgccacggcg tggccgccgg
     1201 agtcgcggcc gccgagccgt tcatgctcgt ccgccaggtg caccacgagg ggggcctgtc
     1261 cagtgatcac cggttctgac atcgcgcccg gcaagcggct cttcgacgtc gccgtctccc
     1321 tgaccgccct ggtcctgctc gtgcccgtcc tggtggtcat cgccctgctg gtcaagttcg
     1381 gctcgcccgg cccggtgctg taccgccagc agcgggtgag ccgcggcggc cggctgttcg
     1441 agctgctcaa gttccgcacc atggtggtcg gcgccgaccg catgtccgcc aacgtcagcg
     1501 ccaccggcga cccgcgcgtc acccctgtcg gcgcgttcct gcgcaagtcc tatctcgacg
     1561 agctgcccca gctgctcaac gtcctgcggg gcgacatgag cctggtcggg ccccggccgg
     1621 agacgccgga gttcgtcgcc ctctacaacc ccgaggagcg gcgggtgctg accgtccggc
     1681 ccggcctggc cggcccctcg accctggcct tcatggacga ggcggacctg ctggccgcgg
     1741 cgcccgaccc ggtcgccttc tacacgacca cggtcctcca cgaccgcgtc cgcgccgacc
     1801 tcgcctacct cgagcggcgg tccatcggct acgacatccg cctgctggcc acccaggtcc
     1861 tcagcatcgt gaggcggctg cgatgacgac catgcccccg gcaccgccgg tccaggtcga
     1921 gcggtggacg aacggcgccg cctgggacca gttcgtgggc ggcgcccgcg acgccaacat
     1981 cggccaccgc tgggcctggc tggggatcgt ctcggaggtg taccggcacc ggaccttccc
     2041 gctggccgcc atccgggacg gggtcctggc cggcgtgctc cccctggtcc tggtccgcag
     2101 ccgcctgttc ggccgccatc tggtgtccat gccgttcctc gactccggcg ggctctgcac
     2161 cgacggcgac gtcgcctccg agcaggcgct ggtcgcgacc gcggtcgagc tggccggcga
     2221 ccacggggcc aagctcgagc tgcgccacct caccgaacgg cggatcggcc tgccggcctc
     2281 gctggagaag gtgaccatga ccctcgacct caccggcggc gaggacgccg tatggcggag
     2341 catccggtcc accaggcggg gccaggtccg caaggcgcgc cgcaacgggc tcgaggcgag
     2401 ctggcacggc caggaggcgg tgggccagtt ctaccgtgtc tgggcggcca acatgcgcga
     2461 cctggggtcc ccggcgcacc ggcgggccta cttcgcccgg atcgccgcca gcctccccgg
     2521 cgacgcccgg ttcgtcctcg tccgggacgg cgccgagacc gtcggcgcgg gtctggcgct
     2581 ggtccacggc gaccgcatcg tcctgccctg ggtgtcgtcg ctgcggtcca gcttctcccg
     2641 cggccccaac cagctgctct actgggaggc cttccgctac ggcatcgaac gcggctgcac
     2701 cgtcttcgac ttcgggcggt cgtcctggaa ctcggggacc ttcgaggcca agcggcagta
     2761 cggcgccgag cccgagcagc tctactggca ccggttcccg gccgaggagg ggaccggcgg
     2821 cgacggcgcc gacaagctgg aatgggcgac ccgggtgtgg aagcggctgc cggtgcccgt
     2881 ggccaacacc gccggcgccc tggtccgcgg agggctgccc aattgagccc cgacctgcgt
     2941 tactctcgac atgtagcgaa gccaccgtca gaccttggtg ggctgcggcc agggggacga
     3001 gatgccacca ggagggccgg caggtgaggc gatcgcgtcc gccctggtcg cggacgtggc
     3061 cggcctgccc cttcccgtcg tcctggccgc cggtggtgtc gtcctcctga tcggggtgtt
     3121 cacggccagg gccctccggg cccgggtgag caaggtcgac gacgtcgagg gctcgaccgg
     3181 cctggtggtc atcggggagg tcccggccag gagcctcggc cggcccatgc cgtccatgaa
     3241 cggcctcccg ctgcggccgg tggacggcct gcaggagatc tgccggatgc tggagcacaa
     3301 cggcctcggg acggccatca cggtgctcac gatcgtccgc gccgggatcc gcggccccga
     3361 ggcgagcttc gcctccgacc tgacccagac cctcgccgcc cagggcaaga acgtcctcct
     3421 cgtccaggcc aacctgcgcc agccgtcggg gcagacggcc atgggcctgg cccaggccga
     3481 aggcctcgcc gacctgctcg aggacgagga cctcgacccc gtgaccctgc tcgtgtcggt
     3541 ggcccagcgc ctgctggtgc tgccctcggg gaccccgcgc tcggacccgg ccgcgctgct
     3601 ggccaggccg cggctcaagc aggtgatcgg caccctccgg gacctcgggt tcatcgccat
     3661 catcgacgcg ccgccggccg ccttcgcccg cgacgtcctc tcgctggccc gcgagggcga
     3721 cgcgacgctg ctgatcgtcc gcaccggcag ccgctggagg gacgtcgagg cggcggccag
     3781 gatgctccgc tacggcgacg tcagcgaccc ggccgccgtg gtcgtcggct cgcggtggtg
     3841 aggcgccggc gcgcctggct cctggcggcg gtggcggcgg gtgccgtcct cgtggcggcc
     3901 gtggccggga tcaggagccg cgccctccag ggctcccccg agccggggac cacccgctac
     3961 gtctcggtcg acggcagcga cggcaacccc ggctccctga gcaggccctg gcggacgatc
     4021 gcgttcgccg ccggccagct ccgccccggc gacaccctcc tcatccgcgg cgggacctac
     4081 ctggagctcc ccacggtcac ggccagcggg accgccgccg ccccgatcac catccagtcc
     4141 tatcccgggg agagcgcggt catcgactcc ggcccggcgt agaagggcgt cgcctggtcg
     4201 acgtaggcgt cgctgggcgc ccgga
//
LOCUS       DASSWR010000020         7236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1244144, whole genome shotgun sequence.
ACCESSION   DASSWR010000020 DASSWR010000000
VERSION     DASSWR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244144"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..267
                     /gene="rplW"
                     /locus_tag="VFO47_00345"
     CDS             1..267
                     /gene="rplW"
                     /locus_tag="VFO47_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET8787178.1"
                     /translation="MYKPVVSEKSYALLDENTYTFIVDPDANKTEIKEAIQAIFSVRV
                     ANVNTLHRKGKRKRTRRTLGVRKATKRALVTLHPDDKIEIFEAR"
     gene            270..1100
                     /gene="rplB"
                     /locus_tag="VFO47_00350"
     CDS             270..1100
                     /gene="rplB"
                     /locus_tag="VFO47_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET8787179.1"
                     /translation="MGIRKHKPTTPGRRGSSVADFVEVTKTTPEKALLAPSPKKGGRN
                     VHGRITARHQGGGHKQRYRIVDFRRTKDGVPAKVAAIEYDPNRTARLALLHYRDGEKR
                     YIIAPTRLKVGDVVESGEGADIKPGNALPLKNIPVGTIVHNVELRPGGGAKMGRSAGS
                     GIQLLAKEGTLATLRMPSGEIRQVLTTCRATVGEVGNAEQELISWGKAGRNRWKGKRP
                     TTRGVAMNPVDHPLGGGEGKSSGGRHPTSPWGKPEGRTRRRKPSDQLIVRRRRKGRGR
                     "
     gene            1103..1384
                     /gene="rpsS"
                     /locus_tag="VFO47_00355"
     CDS             1103..1384
                     /gene="rpsS"
                     /locus_tag="VFO47_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET8787180.1"
                     /translation="MARSLKKGPFVDDHLLKKIDELNRKRDKRVVRTWSRRSTVIPDM
                     VGHTIAVHDGRKHVPVYISEAMVGHKLGEFAPTRTFRIHRSQERATGVR"
     gene            1392..1733
                     /gene="rplV"
                     /locus_tag="VFO47_00360"
     CDS             1392..1733
                     /gene="rplV"
                     /locus_tag="VFO47_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET8787181.1"
                     /translation="MEVKAQARFVRVTPFKARRVLDLIRGLHVEEARRILQFNPRAAA
                     LPVRKVLDSAVANAEHNFQLPADNLFVAKAFVDEGPTLKRHRPRALGRAYRVNKRTSH
                     ITVVVDSREAS"
     gene            1733..2584
                     /gene="rpsC"
                     /locus_tag="VFO47_00365"
     CDS             1733..2584
                     /gene="rpsC"
                     /locus_tag="VFO47_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET8787182.1"
                     /translation="MGQKINPYGFRLGVTTDWKSRWFSEKDYKKYLHEDIEIRRYLKR
                     NLSHAGISRVEIERTRDRVRVDIHTARPGVVIGRRGAEADRIRAALDKMTGKQVTLNI
                     LEVKGAETDAQLVAQGVAEQLASRVSFRRAMRRAINSAMKGGAKGIRVQCSGRLGGAE
                     MARTEFYREGRVPLHTLRADIDYGLAEGRTTFGMVGVKVWIYRGDVLPSREEQEADRA
                     RARARAAAGGPSGERRSGRGRGEGNGGGRSGGRASKAEPASEAPAEAPAEAPAEAEAV
                     PAAEGKE"
     gene            2592..3027
                     /gene="rplP"
                     /locus_tag="VFO47_00370"
                     /pseudo
     CDS             2592..3027
                     /gene="rplP"
                     /locus_tag="VFO47_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
     gene            3008..3223
                     /gene="rpmC"
                     /locus_tag="VFO47_00375"
     CDS             3008..3223
                     /gene="rpmC"
                     /locus_tag="VFO47_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET8787183.1"
                     /translation="MTMKASELADLDADELAAKLAEAKEELFNLRFQNVTGQLDNPHR
                     LREVRKDIARVLTVMHQRERATERERQ"
     gene            3220..3504
                     /gene="rpsQ"
                     /locus_tag="VFO47_00380"
     CDS             3220..3504
                     /gene="rpsQ"
                     /locus_tag="VFO47_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET8787184.1"
                     /translation="MTEQDATTTSKPRGTRKVRTGVVVSDKMDKTVVVEVEDIRQHRL
                     YQKTIRRTTRLKAHDEQNSAGTGDLVRLAETRPMSATKRWRVTEIIQKAK"
     gene            3570..3938
                     /gene="rplN"
                     /locus_tag="VFO47_00385"
     CDS             3570..3938
                     /gene="rplN"
                     /locus_tag="VFO47_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET8787185.1"
                     /translation="MIQQESRLKVADNTGAKEILCIRVLGGSGRRYASIGDIIVATVK
                     DAIPGGAVKEGEVVKAVVVRVKKQKRRPDGSYIRFDENAAVIINEQQNPRGTRIFGPV
                     GRELREKRFMKIISLAPEVL"
     gene            3941..4255
                     /gene="rplX"
                     /locus_tag="VFO47_00390"
     CDS             3941..4255
                     /gene="rplX"
                     /locus_tag="VFO47_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET8787186.1"
                     /translation="MKVRKDDRVMVMAGKDLGVTGRVIKVDPRRNKVLVEGVNRIKRH
                     EKIRPTQRGGQTGGITEKEAWIDASNVQLLSPDDGKPTRVAYRDEDGVKVRVCARTGA
                     KF"
     gene            4271..4840
                     /gene="rplE"
                     /locus_tag="VFO47_00395"
     CDS             4271..4840
                     /gene="rplE"
                     /locus_tag="VFO47_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET8787187.1"
                     /translation="MTQTAPEREMPRLKARYRAEVEQRLKDELGLGNIMQVPRPLKVV
                     VNMGIGEAVKESRLLDGAVRDLTQITGQKPLVTKAKKSIAGFKIREGQSIGAKVTLRG
                     DRMWEFLDRLISVVLPRIRDFRGLNPRTFDGHGNFTFGLTEQLVFPEIDYDKIEKIRG
                     MDITVVASAETDDQGRALLTALGFPFRQS"
     gene            4901..5086
                     /locus_tag="VFO47_00400"
     CDS             4901..5086
                     /locus_tag="VFO47_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET8787188.1"
                     /translation="MAKKALIAKAKRTPKFKVRGYTRCQRCGRPRAVLRKFGLCRICV
                     RQLAHAGELPGVTKSSW"
     gene            5102..5500
                     /gene="rpsH"
                     /locus_tag="VFO47_00405"
     CDS             5102..5500
                     /gene="rpsH"
                     /locus_tag="VFO47_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET8787189.1"
                     /translation="MSMTDPIADMLTRLRNGNAAYHDEIVMPSSRMKVEIAKVLKQEG
                     YIRDYAVEKADVGSKLTVVLKYGPNRERTISGVRRISKPGLRVYAKKESLPRVLGGLG
                     IAIISTSSGLLTDRAAHKAGIGGEVVAYVW"
     gene            5513..6049
                     /gene="rplF"
                     /locus_tag="VFO47_00410"
     CDS             5513..6049
                     /gene="rplF"
                     /locus_tag="VFO47_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET8787190.1"
                     /translation="MSRIGKLPIEVPSGVEVTVEGTLATVKGPMGTLAETIPTSISVR
                     QEGNQLLVERPDDEREHRALHGLVRSLVANMVEGVTKGFEKQLEIQGVGYRVQAQGSD
                     LVFSLGYSHQIPVKAPEGITFEVASPTRFSVKGIDKQQVGQVAADIRRLRKPDPYKGK
                     GVRYAGEVVRRKAGKTAK"
     gene            6060..6413
                     /gene="rplR"
                     /locus_tag="VFO47_00415"
     CDS             6060..6413
                     /gene="rplR"
                     /locus_tag="VFO47_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET8787191.1"
                     /translation="MNNAKVKRDARLRRHRRVRRRVVGTAERPRLAVFRSNKQIYAQL
                     IDDAAGRTLAAAGSPTSAGDGDKKAAATRVGAELATKAKAAGITSVVFDRGGYQYQGR
                     VRALAEAAREGGLKF"
     gene            6439..7083
                     /gene="rpsE"
                     /locus_tag="VFO47_00420"
     CDS             6439..7083
                     /gene="rpsE"
                     /locus_tag="VFO47_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET8787192.1"
                     /translation="MAPAPRRGPGGQQGNRPPRDDRGGPGRDEAPKSQFEERVVAINR
                     VAKVVKGGRRFSFTALVVVGDGNGKVGVGYGKAKEVPSAIQKGVETAKKNFFPVPMIG
                     STIPHEVLGEDGAGRVLLKPASPGTGVIAGGPVRAVLEAAGVSDVLSKSLGSSNPINI
                     VHATVAGLKQLRRPQEVADRRGLDLAEMSPVTVARQKAAAAVGAGAGKAKEQSA"
     gene            7080..>7236
                     /gene="rpmD"
                     /locus_tag="VFO47_00425"
     CDS             7080..>7236
                     /gene="rpmD"
                     /locus_tag="VFO47_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET8787193.1"
                     /translation="MTTRLKVTQKRSLIGRPRPQRDTIRSLGLKRIGDTVVKEDRPEI
                     RGMIFKVS"
BASE COUNT         1312 a         2533 c         2535 g          856 t
ORIGIN      
        1 atctacaagc cggtggtcag cgagaagtcc tacgccctgc tggacgagaa cacctacacc
       61 ttcatcgtcg accccgacgc caacaagacg gagatcaagg aggccatcca ggccatcttc
      121 tccgtccggg tcgcgaacgt gaacaccctg caccgcaagg gcaagcgcaa gcgcacccgc
      181 cgcaccctcg gggtccgcaa ggcgaccaag cgcgcgctgg tcaccctcca ccccgacgac
      241 aagatcgaga tcttcgaggc caggtagcca tgggcatccg caagcacaag ccaaccacgc
      301 cgggacggcg gggcagctcg gtggccgact tcgtggaggt caccaagacg acccccgaga
      361 aggcgctgct ggcaccgagc ccgaagaagg gtggccgcaa cgtccacggg cgcatcaccg
      421 cgcgccacca gggcggcggc cacaagcagc gctaccggat cgtcgacttc cgccggacca
      481 aggacggcgt gccggccaag gtggcggcga tcgagtacga cccgaaccgc accgcccgcc
      541 tggccctgct gcactaccgg gacggcgaga agcgctacat catcgccccc acccgcctca
      601 aggtcggcga cgtcgtcgag tcgggcgagg gcgccgacat caagccgggc aacgccctgc
      661 cgctgaagaa catccccgtc ggcaccatcg tccacaacgt cgagctccgg ccgggcggcg
      721 gcgccaagat gggccgctcc gccgggtccg ggatccagct gctggccaag gagggcacgc
      781 tggccaccct gcggatgccc tcgggcgaga tccgccaggt gctgaccacc tgccgggcca
      841 ccgtcggcga ggtcggcaac gccgagcagg agctgatcag ctggggcaag gccggccgca
      901 accgctggaa gggcaagcgc ccgaccaccc gcggcgtggc catgaacccg gtcgaccacc
      961 cgctcggcgg tggcgagggc aagagctccg gcggccgtca cccgaccagc ccgtggggca
     1021 agccggaggg tcgcacccgc cggcgcaagc cctccgacca gctcatcgtc cgccgccgcc
     1081 gcaaggggag agggcgctag gtatggcacg cagtctgaaa aagggtccct ttgtggacga
     1141 ccatctgctg aagaagatcg acgagctcaa ccgcaagcgc gacaagcggg tggtccgcac
     1201 ctggtcgcgg cgctcgacgg tcatccccga catggtcggg cacacgatcg cggtccacga
     1261 cgggcgcaag cacgtgcccg tgtacatctc ggaggccatg gtcggccaca agctgggcga
     1321 gttcgccccg accaggacct tccgcatcca ccgctcgcag gagcgggcga caggagtgag
     1381 gtaaccccga tatggaggtc aaagcgcagg ctcggttcgt ccgggtgacg ccgttcaagg
     1441 cgcgccgggt cctggacctg atccgcggcc tgcacgtcga ggaggcgcgc cggatcctgc
     1501 agttcaaccc gcgggcggcc gcgctgccgg tacgcaaggt gctcgactcg gccgtcgcca
     1561 acgccgagca caacttccag ctgccggccg acaacctgtt cgtggccaag gcgttcgtcg
     1621 acgagggccc gaccctgaag cggcaccggc cccgcgcgct gggccgcgcc taccgggtga
     1681 acaagcggac cagccacatc acggtcgtcg tcgactccag ggaggcgtcg taatgggaca
     1741 gaagatcaac ccctacggct tccgcctggg cgtcaccacc gactggaagt cgcgctggtt
     1801 cagcgagaag gactacaaga agtacctgca cgaggacatc gagatccgcc ggtacctgaa
     1861 gcggaacctg agccacgccg gcatctcccg ggtggagatc gagcgcaccc gcgaccgggt
     1921 cagggtcgac atccacaccg cccgcccggg cgtggtcatc ggccgccgtg gcgccgaggc
     1981 cgaccgcatc agggccgccc tcgacaagat gaccggcaag caggtcaccc tgaacatcct
     2041 cgaggtcaag ggcgccgaga ccgacgccca gctcgtggcc cagggcgtgg ccgagcagct
     2101 ggccagccgg gtgtcgttcc gccgggccat gcgccgggcc atcaactcgg ccatgaaggg
     2161 cggggccaag ggcatccggg tgcagtgctc cggccgcctg ggcggcgccg agatggcccg
     2221 caccgagttc taccgcgagg gccgcgtgcc cctgcacacc ctgcgggccg acatcgacta
     2281 cggcctggcc gagggccgca ccaccttcgg gatggtcggg gtcaaggtct ggatctaccg
     2341 cggcgacgtg ctgccctcgc gcgaggagca ggaggccgac cgggcccggg cccgcgcccg
     2401 ggccgccgcc ggcgggccca gcggcgagcg ccgttcgggt cgcggccggg gcgagggcaa
     2461 cggcggcggc cgctccggcg gccgcgcctc caaggccgag ccggccagcg aggcgcccgc
     2521 cgaggctccg gccgaggctc cggccgaggc cgaggccgtc ccggcggcag agggcaagga
     2581 gtgaccgacc gatgctgacc cccaagcgcg tcaagcaccg caagcagcac cgcggccacc
     2641 gccgcggcgt ggccagcggc ggcaccagcg tggccttcgg cgactacggc ctgcaggcgc
     2701 tggagccctc ctgggtgacc aaccggcaga tcgaggccgc ccgtatcgcc atgacccgct
     2761 acatcaagcg tggcggcaag gtctggatca atgtctaccc ggacaagccg gtgaccaaga
     2821 agcccgccga gacccgcatg ggctcgggca agggctcgcc ggagtggtgg gtcgcggtgg
     2881 tccggccggg ccgggtgatg ttcgagctgt cgggcgttcc cgaggccacc gcccaggagg
     2941 cgatgcgcct ggccgcccac aagctgccga tgaagtgcaa gttcgtgaag cgcgttgacg
     3001 cggagtgatg accatgaagg caagtgagct ggccgacctg gacgcggacg agctggccgc
     3061 caagctggcc gaggccaagg aggagctgtt caacctccgg ttccagaacg tcaccggcca
     3121 gctcgacaac ccgcaccggc tgcgcgaggt ccgcaaggac atcgccaggg tcctcaccgt
     3181 gatgcaccag cgcgagcggg ccacagagag ggagcgccag tgaccgagca ggacgcgacg
     3241 accacgagca agccccgcgg gacccgcaag gtccgcaccg gggtcgtggt ctccgacaag
     3301 atggacaaga cggtggtcgt cgaggtggag gacatccgcc agcaccgcct gtaccagaag
     3361 acgatccggc ggacgacccg gctcaaggcc cacgacgagc agaacagcgc cggcacgggc
     3421 gacctcgtcc ggctggccga gacccggccc atgtcggcga ccaagcgctg gcgggtcacc
     3481 gagatcatcc agaaggcgaa gtgaaccgca ggttcacttc gcagaaccat gggacaacgc
     3541 cgaccacgga agatgaataa gaggtctgta tgatccagca ggagtcgcgg ctgaaggtcg
     3601 cggacaacac gggtgccaag gagatcctct gcatccgcgt gctcggcggc tccgggcggc
     3661 gctacgcctc catcggcgac atcatcgtgg ccacggtgaa ggacgccatc cccggtggcg
     3721 ccgtcaagga gggcgaggtc gtcaaggcgg tcgtcgtccg ggtcaagaag cagaagcgcc
     3781 gccccgacgg ctcctacatc cgcttcgacg agaacgcggc cgtgatcatc aacgagcagc
     3841 agaacccgcg gggcacccgc atcttcgggc cggtcggccg ggagctgcgg gagaagcgct
     3901 tcatgaagat catcagcctg gccccggagg tgctgtagcc gtgaaggtac gcaaggacga
     3961 ccgcgtgatg gtcatggccg gcaaggacct cggcgtcacc gggcgggtga tcaaggtcga
     4021 cccgcgccgc aacaaggtgc tggtcgaggg cgtcaaccgc atcaagcggc acgagaagat
     4081 ccggcccacc cagcggggcg gccagaccgg cggcatcacc gagaaggagg cctggatcga
     4141 cgcctccaac gtgcagctgc tcagccccga cgacggcaag ccgacccggg tcgcctaccg
     4201 cgacgaggac ggcgtcaagg tccgggtctg cgcccgcacc ggcgcgaagt tctaagagga
     4261 agacgagacg atgacgcaga ccgctccaga gcgagagatg ccgcggctga aggcgcgcta
     4321 ccgcgccgag gtcgagcagc ggctcaagga cgagctcggg ctcggcaaca tcatgcaggt
     4381 cccgcggccg ctcaaggtcg tggtcaacat gggcatcggc gaggccgtca aggagtcccg
     4441 cctgctggac ggcgcggtgc gcgacctgac ccagatcacc gggcagaagc cgctggtcac
     4501 caaggccaag aagtcgatcg ccggcttcaa gatccgcgag ggccagtcca tcggggccaa
     4561 ggtcaccctg cgcggcgacc gcatgtggga gttcctggac cggctgatct cggtcgtcct
     4621 gccccgcatc cgcgacttcc gcggcctcaa cccaaggacc ttcgacggcc acggcaactt
     4681 caccttcggg ctgaccgagc agctggtctt cccggagatc gactacgaca agatcgagaa
     4741 gatccggggc atggacatca ccgtggtggc cagcgccgag accgacgacc aggggcgggc
     4801 gctgctcacc gccctcgggt tcccgttccg ccagagttga cgacggttcc cgccaggccg
     4861 gacacccgga accggcggga ccaggaggta gcaccagcag atggcaaaga aggcactgat
     4921 cgccaaggcc aagcgcaccc cgaagttcaa ggtgcgcggc tacacgcgct gccagcgctg
     4981 cgggcggccg cgggccgtgc tgcgcaagtt cggcctgtgc cggatctgcg tgcgccagct
     5041 cgcccacgcc ggcgaactgc cgggcgtcac caagtcgagc tggtaggggg cgggtactga
     5101 catgagcatg accgacccga tcgccgacat gctgacccgg ctgcgcaacg gcaacgcggc
     5161 ctaccacgac gagatcgtca tgcccagctc gaggatgaag gtggagatcg ccaaggtcct
     5221 caagcaggag ggctacatcc gggactacgc ggtcgagaag gccgacgtcg gttccaagct
     5281 gacggtcgtc ctcaagtacg gccccaaccg ggagcggacc atcagcggcg tccgccgcat
     5341 ctccaagccg gggctgcgcg tgtatgccaa gaaggagagc ctgccccggg tcctgggagg
     5401 cctcggcatc gccatcatct cgacctcgtc cggcctgctc accgaccgcg ccgcccacaa
     5461 ggccggcatc ggtggcgagg tcgtggccta cgtctggtaa gggaggcgca cgttgtcacg
     5521 catcggcaag cttcccatcg aggtccccag cggcgtcgag gtgacggtcg agggcaccct
     5581 ggccaccgtg aagggcccca tgggcaccct ggccgagacg atcccgacgt ccatctcggt
     5641 ccgccaggag ggcaaccagc tcctggtcga gcggcccgac gacgagcgcg agcaccgggc
     5701 cctgcacggc ctggtccgct ccctggtggc caacatggtc gagggggtca ccaaggggtt
     5761 cgagaagcag ctcgagatcc agggggtcgg ctaccgggtc caggcccagg gctccgacct
     5821 ggtgttcagc ctcggctact cccaccagat cccggtcaag gcccccgagg gcatcacctt
     5881 cgaggtggcc agcccgaccc ggttctcggt caagggcatc gacaagcagc aggtgggcca
     5941 ggtggcggcc gacatccggc ggctgcgcaa gccagacccc tacaagggca agggcgtgcg
     6001 ctacgccggc gaggtcgtcc gccgcaaggc cggcaagacg gcgaagtgag gacccgacga
     6061 tgaacaacgc aaaggtgaag cgcgacgcgc gcctgcggcg tcaccgtcgc gtgcggcggc
     6121 gggtggtggg cacggccgag cggccgcgcc tggccgtctt ccgctccaac aagcagatct
     6181 acgcccagct catcgacgac gcggccggcc gcacgctggc cgcggctggc tcgccgacct
     6241 cggccggcga cggcgacaag aaggccgccg ccacccgggt cggggccgag ctggcgacca
     6301 aggccaaggc ggccggcatc acctcggtgg tgttcgaccg cggcggctac cagtaccagg
     6361 gacgtgtccg cgcgctggcc gaggcggccc gcgagggcgg tctcaagttc taggaagatc
     6421 ctgaaggagg cattccgcat ggcaccagcc cctcgccggg gtcccggtgg acagcaggga
     6481 aaccggccgc cccgtgacga ccggggcggc ccaggccggg atgaggcccc caagtcccag
     6541 ttcgaggagc gcgtggtcgc gatcaaccgc gtggccaagg tcgtgaaggg tggccgccgg
     6601 ttctcgttca ccgccctggt ggtggtcggc gacggcaacg gcaaggtcgg cgtcggctac
     6661 ggcaaggcca aggaggtccc ctcggccatc cagaagggtg tcgagacggc caagaagaac
     6721 ttcttcccgg tgccgatgat cggctcgacc atcccccacg aggtcctcgg tgaggatggc
     6781 gccggccggg tcctgctcaa gccggcctcc ccgggtaccg gggtcatcgc cggcggcccc
     6841 gtccgggccg tcctggaggc ggccggggtc tccgacgtgc tctccaagtc gctcgggtcc
     6901 tccaacccga tcaacatcgt ccacgccacc gtggccggcc tgaagcagct gcgccggccc
     6961 caggaggtgg ccgaccgccg cgggctcgac ctggccgaga tgtcgccggt caccgtggcc
     7021 cggcagaagg cggcggccgc ggtcggcgcc ggcgccggca aggcgaagga gcagtcggcg
     7081 tgacgacccg tctcaaggtc acccagaagc ggtccctgat cggccggccc cgaccgcagc
     7141 gggacacgat caggagcctg gggctcaagc ggatcggcga caccgtcgtc aaggaggacc
     7201 gccccgagat ccgcggcatg atcttcaagg tctccc
//
LOCUS       DASSWR010000021         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1244203, whole genome shotgun sequence.
ACCESSION   DASSWR010000021 DASSWR010000000
VERSION     DASSWR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244203"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFO47_00430"
     CDS             <1..780
                     /locus_tag="VFO47_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787194.1"
                     /translation="IDCSVRRIQFTPDLLPSDVTGVSVFNQERGDFEFKPGAIFANIV
                     LGDEINRASPKTQAALLESMEERQVTVDGVTYQRGVPFMVIATQNPIEHEGTYPLPEA
                     QRDRFMLELNIGYPTRQAEIEILETHGRTTTLLSDLQPVADAPAVADLIEAAKTVHVA
                     DTLKRYIVDLVRATRDHADLALGASPRAALSLLRAARAAGAVSGRDYVVPDDVKELVT
                     PVLAHRLITAPEAQMIGRTAADVLGDIVEYVPIPIRGRGQG"
     gene            782..2050
                     /locus_tag="VFO47_00435"
     CDS             782..2050
                     /locus_tag="VFO47_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8787195.1"
                     /translation="MLKSFTPRAAGFAGAAVALYLAGRLTGVPELYMLAVAALALPLG
                     ALAVVRWGNARLTSSRAVRPVRTSVGNRIAVTVRLDNPARLETGVLLLEDRLPYQLGP
                     GARFVVPGIPGGDRELLHYELRAASRGRYSIGPLAVRLADPFGLAQVTSELAGASDVI
                     VHPRVEALSAPGLGGELASSAATKVRYLFSQGDEFYTTRDYRDGDDLRKVHWRSSAKR
                     GQLMIRQEERPWQARAVIAIDLRLAAHRGQGGRASFERAVSAAASIALRLGRSGYELA
                     LVADDGRRVSPAGLADQATAVLDFLASVEPTSTPSLVPMANRLARTAGEGLLVAVLGV
                     PSSDEAAALARCRLGFGGALGLLVRTDSWLGLAAREVAAADAQAAGIATLLDRAGWRT
                     AMLSRTQPLDDPWRRLTAPLGRATLTSHRH"
     gene            1993..>2724
                     /locus_tag="VFO47_00440"
     CDS             1993..>2724
                     /locus_tag="VFO47_00440"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET8787196.1"
                     /translation="MATTDSAPGARNTDFAQALALAGLLAGASLSLSRLYAGNSWLVP
                     TWLTMAAALGLGALLRRLGVGQLLALLAMVAGFLVVGGILLFPDTLLLVVPTGETLSA
                     MATAASTGLAEVSEQAAPVQVTTGFLLLTCAGAWAVATAADGLAFRARQPLLALVPAL
                     GLFVFPAVIRPTSPAWYTAWFLGGAAGMLLFEGRARLATWGRWVSHARSRPGVGWRLP
                     MTPAAQTGRWLALAAGLCALTVPWLL"
BASE COUNT          296 a         1086 c          972 g          370 t
ORIGIN      
        1 atcgactgct cggtccgccg catccagttc acccccgacc tgctgccctc cgacgtcacc
       61 ggggtcagcg tgttcaacca ggagcggggg gacttcgagt tcaagccggg ggcgatcttc
      121 gccaacatcg tcctcggcga cgagatcaac cgggcctccc ccaagaccca ggccgccctg
      181 ctcgagtcca tggaggagcg gcaggtcacc gtggacgggg tcacctacca gcggggcgtg
      241 cccttcatgg tgatcgccac ccagaacccg atcgagcacg agggcaccta tcccctgccc
      301 gaggcccagc gcgaccggtt catgctcgag ctcaacatcg gctacccgac ccggcaggcc
      361 gagatcgaga tcctggagac ccacggccgg accaccaccc tgctcagcga cctccagccg
      421 gtggccgacg cgccggcggt ggccgacctg atcgaggccg ccaagaccgt gcacgtggcc
      481 gacaccctca agcgctacat cgtcgacctg gtccgggcga cccgcgacca cgccgacctg
      541 gccctggggg cgagcccgcg ggccgccctg tccctgctgc gggcggcccg ggccgccggg
      601 gccgtgtccg ggcgcgacta cgtggtcccc gacgacgtca aggagctggt caccccggtc
      661 ctggcccacc ggctgatcac cgcccccgag gcccagatga tcggccgcac cgccgccgac
      721 gtcctgggcg acatcgtcga gtacgtcccc atcccgatcc gcggccgggg gcagggctag
      781 cttgctgaag tcgttcaccc cgcgggcggc cgggttcgcc ggggcggcgg tcgcgctgta
      841 cctggccggc cgcctcaccg gcgtccccga gctgtacatg ctggccgtgg ccgccctcgc
      901 cctccccctg ggggcgctgg ccgtggtccg gtggggcaac gcccggctca cctccagccg
      961 ggcggtgcgg ccggtgcgga cctcggtcgg caaccggatc gccgtcaccg tgcgcctcga
     1021 caacccggcc cggctggaga cgggcgtgct gctgctcgag gaccgcctcc cctaccagct
     1081 cggccccggg gcccgcttcg tcgtccccgg gatccccggc ggcgaccggg agctgctcca
     1141 ctacgagctg cgggcggcct cccggggccg gtactcgatc gggccgctgg ccgtccgcct
     1201 ggccgacccg ttcgggctgg cccaggtcac ctcggagctg gccggggcct cggacgtgat
     1261 cgtccacccg cgggtcgagg ccctcagcgc gcccgggctg ggcggggagc tggcctcctc
     1321 ggccgcgacc aaggtccgct acctgttctc ccagggcgac gagttctaca ccacccgcga
     1381 ctaccgcgac ggcgacgacc tgcgcaaggt ccactggcgc tcctcggcca agcggggcca
     1441 gctgatgatc cgccaggagg agcgcccctg gcaggccagg gccgtgatcg ccatcgacct
     1501 gcgcctggcc gcccaccgcg gccagggggg ccgggcctcg ttcgagcggg cggtgtcggc
     1561 ggccgcctcg atcgccctgc gcctgggccg ctccggctac gagctcgccc tggtggccga
     1621 cgacgggcga agggtgagcc cggccggcct ggccgaccag gccacggccg tgctcgactt
     1681 cctggccagc gtcgagccga cctcgacccc gtcgctggtg ccgatggcca accggctggc
     1741 ccggacggcc ggcgaggggc tgctggtggc cgtgctcggc gtcccctcct ccgacgaggc
     1801 ggccgccctg gcccgctgcc ggctcggctt cggcggcgcc ctcggcctgc tggtccgcac
     1861 cgacagctgg ctggggctgg ccgcccgcga ggtggccgcc gccgacgccc aggcggccgg
     1921 catcgccacc ctgctcgacc gggccgggtg gcggaccgcg atgctctccc gaacccaacc
     1981 cctggacgac ccatggcgac gactgacagc gcccctgggg cgcgcaacac tgacttcgca
     2041 caggcactag cgctggccgg cctgctggcc ggcgcctcgc tgtcgctcag ccggctgtac
     2101 gccggcaact cctggctggt cccgacctgg ctgaccatgg ccgccgccct cggcctgggc
     2161 gcgctgctgc gccgcctcgg cgtcggccag ctgctggcgc tgctggccat ggtcgccggc
     2221 ttcctggtcg tcggcgggat cctgctcttc ccggacacgc tgctgctggt cgtccccacc
     2281 ggcgagacgc tgtcggcgat ggccacggcg gcctcgacgg ggctggccga ggtcagcgag
     2341 caggcggccc cggtccaggt gacgaccggg ttcctgctgc tcacctgcgc cggcgcctgg
     2401 gcggtggcca ccgccgccga cgggctcgcc ttccgggccc gccagccgct gctggccctg
     2461 gtccccgccc tggggctgtt cgtgttcccg gccgtcatcc ggcccaccag ccccgcctgg
     2521 tacacggcct ggttcctggg tggggcggcc gggatgctgc tgttcgaggg gcgggcgcgg
     2581 ctggccacct ggggccgctg ggtgtcgcac gcccgcagcc ggcccggggt gggttggcgg
     2641 ctgccgatga ccccggccgc ccagaccggc cgctggctgg ccctggccgc cgggctgtgc
     2701 gccctcaccg tgccctggct gctg
//
LOCUS       DASSWR010000022         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_311753,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000022 DASSWR010000000
VERSION     DASSWR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311753"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VFO47_00445"
     CDS             <1..1308
                     /locus_tag="VFO47_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787197.1"
                     /translation="AENAALRAQVGHGGADEDTARLPGRPAARQRWRTVVATLLIVVG
                     CVLAPLSVAAVWTRNQVTNTDRYVATVSPLADDPAIQSAVADQITAQVFTYIDVQGLT
                     NQAVDALAERGLTPVLAEQLRALSVPIANGVQSFTRSQVGRVVESDAFADAWVQANRV
                     AHQELVAALTGEGGGSVTVENDTVSLNMAAFIETVKQRLVESGFSVAARIPTVNASFV
                     LFQSEDITRARSGFNLLNTLGVWLPVIAIVLLVLGVYVARDHRRATVGAALGVAISMV
                     VLALGLAVFRSIYLDAVPASVLPHDAAAVLYDTIIRFLRLGIRTVFVLALVVAAGAFL
                     SGQSVTAVRTREGLSHAIGWLAGGAERAGFSTGPVGTWVYANKRVLRVGAVALAALAL
                     VFWGRPTGKVVLGLTLALLVVLAIIEFLGRPARPAADVAAGQA"
     gene            complement(1313..1813)
                     /locus_tag="VFO47_00450"
     CDS             complement(1313..1813)
                     /locus_tag="VFO47_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787198.1"
                     /translation="MSEAPARPSRWRVFAPDTAGAIYGTIAAMAVIAGSASDPEHAKP
                     LQLALATLFVFWLAHVYAQALSHHLRGAKRLDWSVVTGAMAEERPLLEGPLPILAVVA
                     LGTFGILELRQAIRLALWLGVIELVSWGILYARRQRWRWLTALTAGAINGFFGLAIVL
                     LEVVVH"
     gene            complement(1881..3905)
                     /locus_tag="VFO47_00455"
     CDS             complement(1881..3905)
                     /locus_tag="VFO47_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HET8787199.1"
                     /translation="MRTLNLGILAHVDAGKTTLTERLLFAAGVIDEVGSVDDGSTQTD
                     SLELERQRGITIKSAVVSFVVGDVTVNLIDTPGHPDFIAEVERVLHVLDGVVLVISAV
                     EGVQAQTLVLMRALRRLGIPTLLFVNKVDRVGARDTAVLAGIAERLTPAIIPMGSLSG
                     LGTRRAGFTPFSAADRAFTARLAEVLADHDDALLAAWVDDRAALSYRRLRRALVAQAR
                     RGLVHPVFFGSAITGAGVEPLLAGVTELLPAAQGDPGGPVAGSVFKVERGPAGEKVAY
                     VRMFSGSVRVRDRLPLPRGGEGKVTAISVFDRGAAVRRRAVTAGQIGKLWGLGAVQIG
                     DAVGGSPATLAAPSFARPSLETVVVPRRPADKAALQVALTQLAEQDPLIDLRQDDTRG
                     ELSVSLYGEVQKEVIQATLAGDYGLEVEFRETTTLCVERPVGTGAAAEIIGKDPNPFL
                     ATVGLRVEPAPVGAGVRFLLEVELGSMPYAFFRAVEDTVRETLRQGLHGWEVIDCTVT
                     MTHSGYWPRQSHAHATFDKSMSSTARDFRQLTPLVLMDALAQAGTRVHEPVHRFHLEI
                     PADTLGALSPVLARLHAVPRSTALQGPAYLLDGDILAARVDDLERQLPALTRGEGVLE
                     SAFDHYQPVNGPPPARPRTDHDPLDRKQYLLTVLRRVPGRQVDGGFTPGG"
     gene            4156..>4615
                     /locus_tag="VFO47_00460"
     CDS             4156..>4615
                     /locus_tag="VFO47_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8787200.1"
                     /translation="MPADAGFYAAVAGQLAGAWTVVAYDPRGMSRSRLHGPPEDQRVE
                     VHADDAHRVLAAAGQGPAHVLADSISGLPGLQLAASHPEQVRTLVAFEPPLTELLPDR
                     ERWRAFYAELHATYRDEGLHPALHRFAEAVGLGGEEPPQGDPDPEVTASMA"
BASE COUNT          617 a         1739 c         1657 g          602 t
ORIGIN      
        1 gccgagaacg ccgccctgcg cgcccaggtc ggccacggcg gcgccgacga ggacactgcc
       61 cggctgccgg gtaggccggc ggcccgccag cgctggcgca cggtcgtggc caccctgctg
      121 atcgtggtcg gctgcgtgct cgccccgctg tcggtggcgg cggtgtggac ccgcaaccag
      181 gtcaccaaca ccgaccgcta cgtggccacc gtctcccccc tggccgacga cccggccatc
      241 cagagcgccg tcgccgacca gatcaccgcc caggtgttca cctacatcga cgtccagggt
      301 ctgaccaacc aggccgtcga cgccctggcc gagcgcggcc tgaccccggt gctggccgag
      361 cagctccgcg ccctcagcgt gccgatcgcc aacggggtcc agagcttcac ccgcagccag
      421 gtcggcaggg tggtcgagag cgacgccttc gccgacgcct gggtccaggc caaccgggtc
      481 gcccaccagg agctggtcgc cgccttgacc ggcgagggtg ggggatcggt cacggtcgag
      541 aacgacaccg tgagcctgaa catggccgcc ttcatcgaga cggtcaagca gcggctggtc
      601 gagtccgggt tctcggtcgc ggcccgcatc cccacggtca acgcctcctt cgtgctgttc
      661 cagtccgagg acatcacccg ggcccgcagc ggcttcaacc tgctcaacac cctcggggtg
      721 tggctgcccg tgatcgccat cgtcctgctc gtcctcgggg tgtacgtggc ccgcgaccac
      781 cggcgggcca ccgtcggagc cgccctcggg gtggccatct ccatggtcgt gctggcgctg
      841 gggctggccg tgttccggtc catctacctg gacgcggtgc cggcgtcggt gctgccccac
      901 gacgccgccg ccgtgctgta cgacaccatc atccggttcc tgcgcctggg gatccggacc
      961 gtgttcgtcc tggccctggt cgtggccgcg ggggcgttcc tgtccggcca gtccgtgacc
     1021 gccgtccgga cccgcgaggg cctcagccac gccatcggct ggctggccgg gggagcggag
     1081 cgggccgggt tctcgaccgg cccggtcggg acctgggtgt acgccaacaa gcgggtcctg
     1141 cgcgtcggcg ccgtcgccct ggccgccctg gccctggtgt tctggggccg gcccaccggc
     1201 aaggtcgtgc tcggcctcac cctggccctg ctggtcgtgc tggccatcat cgagttcctg
     1261 ggccgaccgg cccgcccggc cgccgacgtg gccgccggcc aggcctgacc gatcagtgca
     1321 cgaccacctc gagcaggacg atggccagcc cgaagaagcc gttgatggcg cccgcggtca
     1381 gcgccgtcag ccacctccag cgctggcggc gggcgtagag gatcccccag gagaccagct
     1441 cgatcacgcc cagccacagg gccaggcgga tcgcctggcg gagctccagg atgccgaacg
     1501 tccccagggc gaccacggcc aggatcggca gcgggccctc cagcagcggc cgctcctcgg
     1561 ccatggcccc ggtcaccacc gaccagtcca gccgcttcgc cccgcgcagg tggtgggaca
     1621 gcgcctgggc gtacacgtgg gccagccaga acacgaacag ggtggccagc gccagctgca
     1681 gcggcttggc gtgctcgggg tcggacgcgg acccggcgat caccgccatg gccgcgatcg
     1741 tgccgtagat cgcccccgcg gtgtcgggcg cgaacacccg ccagcgggag ggtcgcgcgg
     1801 gtgcctcgga catcgacgct cctcgccagt cgccgtcttg cggtcgggac cgatcgtggc
     1861 gcaagaccga tgcaatcgca tcatccgccc ggggtgaacc ccccgtccac ctgacgcccg
     1921 gggacccggc gcaggaccgt cagcaggtac tgcttgcggt cgagggggtc atggtcggtg
     1981 cgggggcggg ccgggggcgg gccgttgacc ggctggtagt ggtcgaaggc ggactccagc
     2041 accccctcgc cgcgggtcag ggccgggagc tggcgctcca ggtcgtcgac ccgggcggcc
     2101 aggatgtcgc cgtccagtag gtaggccggg ccctggaggg cggtcgaccg ggggacggcg
     2161 tgcagccggg ccaggacggg ggagagggcc ccgagggtgt cggccgggat ctccaggtgg
     2221 aagcggtgga ccggctcgtg gacccgggtg ccggcctggg cgagggcgtc catcagcacc
     2281 agcggcgtca gctggcggaa gtccctggcc gtgctcgaca tgctcttgtc gaaggtggcg
     2341 tgggcgtggc tctggcgcgg ccagtagccg gagtgggtca tggtgaccgt gcagtcgatc
     2401 acctcccagc cgtggagccc ctggcgcagg gtctcgcgca cggtgtcctc gaccgccctg
     2461 aagaacgcgt acggcatcga cccgagctcc acctccagca ggaaccgcac accggcgccg
     2521 accggcgccg gctcgacccg caacccgacg gtggcgagaa agggattcgg gtccttgccg
     2581 atgatctccg ctgcggcgcc ggtcccgacc ggccgctcga cgcacagggt cgtggtctcg
     2641 cggaactcca cctccaggcc gtagtcgccg gccagggtcg cctggatgac ctccttctgg
     2701 acctcgccgt agagcgagac cgacagctcc ccccgggtgt cgtcctggcg caggtcgatc
     2761 agcgggtcct gctcggccag ctgggtgagc gcaacctgga gggcggcctt gtcggccggg
     2821 cggcggggga cgacgaccgt ctccagcgac ggccgggcga acgacggggc tgccagggtc
     2881 gccggcgacc cgccgaccgc gtcgccgatc tggacggcgc ccagccccca gagcttgccg
     2941 atctgcccgg cggtgacagc cctacgccgg acggccgcgc cgcggtcgaa cacgctgatg
     3001 gcggtgacct tcccctcgcc gccccgcggg agcggcagcc ggtcccggac gcgcaccgag
     3061 cccgagaaca tccgcacgta ggcgaccttc tccccggccg ggccccgctc gaccttgaac
     3121 accgagcccg ccaccggacc gcctgggtcg ccctgggccg ccggcaggag ctcggtgacc
     3181 ccggccagca gcggctccac gccggcgccg gtgatggccg agccgaagaa caccgggtgc
     3241 accaaccccc gccgggcctg ggccacgagc gcgcggcgga gccgccgata ggagagtgcc
     3301 gcccggtcgt cgacccaggc cgccaggagg gcgtcgtcgt ggtcggccag cacctcggcc
     3361 agccgggccg tgaacgcccg gtcggcggcg ctgaagggcg tgaacccggc ccggcgcgtg
     3421 cccagcccgg acagcgagcc catcgggatg atcgccggcg tcagccgttc ggcgatcccc
     3481 gccagcacgg cggtgtcccg cgcgccgacg cggtcgacct tgttcacgaa cagcagcgtg
     3541 gggatgccca gccgccgcag cgcccgcatc agcaccaggg tctgcgcctg caccccctcg
     3601 acggccgaga tcaccaggac cacgccgtcg agcacgtgca gcacccgttc cacctcggcg
     3661 atgaagtccg ggtggccggg ggtgtcgatc aggttgacgg tgacgtcgcc gacgacgaac
     3721 gacaccaccg ccgacttgat ggtgatgccg cgctggcgct ccagctccag cgagtcggtc
     3781 tgggtgctgc cgtcatcgac gctgccgacc tcgtcgatga cgccggcggc gaacagcagc
     3841 cgttcggtga gggtggtctt cccggcgtcg acatgggcca ggatccccag gttcaatgtg
     3901 cgcacggagc gtcatgtcct ctggctcgag ggcgaagccc tggtgggcgg acatgcacgc
     3961 cggcgtcatc tcggtctctc ttggtcggtc gggtcgtccc ggccagcaca gcagacgccc
     4021 gtccggcggg caaccgaatt gccaggaggt gggagagcga tcggagcggc gaaggtcgcc
     4081 acgctggagg tcccgggcgc gagcctgcac tacgaggtcc gcgggtccgg ccccgccctg
     4141 ctgatcatcc ccggcatgcc ggccgacgcc gggttctacg cggccgtggc cggccagctc
     4201 gccggcgcct ggacggtggt ggcctacgac ccccggggca tgtcccgcag ccgcctccac
     4261 ggaccgccag aggaccagcg ggtcgaggtc cacgccgacg acgcccaccg cgtcctggcc
     4321 gccgccggcc aggggccggc ccacgtcctc gccgacagca tctccggcct gcccggcctc
     4381 cagctcgccg ccagccaccc cgagcaggtt cgcaccctgg tcgcgttcga gccacccctg
     4441 accgagctgc tgcccgaccg cgagcgctgg cgcgccttct acgccgagct gcacgccacc
     4501 taccgcgacg aggggctcca cccggccctc cacaggttcg ccgaggctgt gggcctgggc
     4561 ggcgaggagc caccccaggg cgaccccgat cctgaggtga cggcctccat ggccc
//
LOCUS       DASSWR010000023         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_792118,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000023 DASSWR010000000
VERSION     DASSWR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792118"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFO47_00465"
     CDS             <1..456
                     /locus_tag="VFO47_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HET8787201.1"
                     /translation="EENWISRLGSEDFGALVDYQDRYQPGARRFDVGQRSHFETTPMA
                     VAALRQLLDWEVERVAATLGQVTGRIQHEVEAIGLRLTSGDRVPHMLGIALPDEARGA
                     VAAALADAGVFAGVRGSSLRVSPHLWTTDQDVERLVGALTKATNRMVAS"
     gene            453..1220
                     /locus_tag="VFO47_00470"
     CDS             453..1220
                     /locus_tag="VFO47_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET8787202.1"
                     /translation="MKTLGTYNIKGGVGKTATAVNLAWLAARDGQRTLLWDLDPQGAA
                     SYVLRVRPHVKGGGKALVRGKRSLDQAVKQTEFERLDLVPSDFTYRNLDLVLDDAKKP
                     TQRLARMLAPLADDYDVAILDSPPGISLVSENIVQAAGTLLVPLIPTTLSVRTLEQLT
                     EFVAGLDHRPALFPFFTMADGRKRLHREVMEDLRARRDDLATTVIPARSLVEQMAVHR
                     APLPTFSPRSQVTRSYEELWAEVRQRSGTAWIPKGGS"
     gene            1217..1597
                     /locus_tag="VFO47_00475"
     CDS             1217..1597
                     /locus_tag="VFO47_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787203.1"
                     /translation="MTQDYVAGACNIGPAEIALRRRVGHVGLATTAVLAAALIRSDLP
                     RPWRLTLALPATGAAAGYLQARSRFCADFGFRGVYNFDRPARRPQAQVPTEQARQQDR
                     RRALRIGAASAAIGVGVAVVALLA"
     gene            1700..1879
                     /locus_tag="VFO47_00480"
     CDS             1700..1879
                     /locus_tag="VFO47_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787204.1"
                     /translation="MSLLVRDNTTIRLQDHWPTQDDLGLLVLMPGGEAGILTSWWHAP
                     DHSEWRWQLELSGHR"
     gene            2122..2253
                     /locus_tag="VFO47_00485"
     CDS             2122..2253
                     /locus_tag="VFO47_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787205.1"
                     /translation="MIHDLPRIAWIVEHRAAELTDEERQIVRDATTRMLHALEHDTV"
BASE COUNT          388 a         1091 c          844 g          371 t
ORIGIN      
        1 gaggagaact ggatctcgcg cctcgggtcg gaggacttcg gcgccctggt cgactaccag
       61 gaccgctacc agccgggggc ccgccgcttc gacgtcggcc agcgcagcca cttcgagacc
      121 accccgatgg ccgtggccgc cctccgccag ctgctcgact gggaggtcga gcgggtcgcc
      181 gccaccctcg gccaggtcac cggccgcatc cagcacgaag tcgaggccat cggcctccgg
      241 ctgacctccg gcgaccgggt cccgcacatg ctcggcatcg ccctccccga cgaggcgcgc
      301 ggggccgtgg ccgccgcgct cgccgacgcc ggcgtgttcg ccggggtcag gggctcgtcc
      361 ctgcgcgtct ccccgcacct gtggaccacc gaccaggacg tggagcgcct ggtcggcgcc
      421 ctgaccaagg cgacgaacag gatggtggcc tcgtgaagac cctggggacc tacaacatca
      481 agggcggggt tgggaagacc gccaccgccg tcaacctggc ctggctggcc gcccgggacg
      541 gccagcggac cctgctctgg gacctcgacc cgcagggcgc ggccagctac gtcctccgcg
      601 tccgccccca cgtcaagggc ggcggcaaag ccctcgtccg cggcaagcgc tccctcgacc
      661 aggccgtcaa gcagaccgag ttcgagcgtc tcgacctggt gccctccgac ttcacctacc
      721 gcaacctcga cctcgtcctc gacgacgcca agaagccgac ccagcggctc gcccgcatgc
      781 tcgccccgct cgcggacgac tacgacgtgg ccatcctcga ctccccgccc ggcatctccc
      841 tggtctccga gaacatcgtc caggccgccg gcaccctgct cgtccccctc atccccacca
      901 ccctgtcggt ccggaccctg gagcagctga ccgagttcgt ggccgggctc gaccaccggc
      961 cggccttgtt ccccttcttc accatggccg acggccgcaa gcgcctccac cgcgaggtga
     1021 tggaggacct ccgcgcccgc cgcgacgacc tggcgaccac ggtcatcccc gcccgctccc
     1081 tggtcgagca gatggccgtc caccgcgccc ccctgcccac cttctccccc cgcagccagg
     1141 tcacccgcag ctacgaggag ctgtgggccg aggtccggca acgcagcggc accgcgtgga
     1201 tcccgaaggg aggctcatga cgcaggacta cgtcgccgga gcctgcaaca tcggcccggc
     1261 cgagatcgcc ctccgccgcc gcgtcgggca cgtcggcctg gccaccaccg ccgtcctcgc
     1321 cgccgccctc atccgctccg acctcccccg gccctggcgc ctcaccctcg ccctcccggc
     1381 caccggcgcc gcggccggct acctccaggc ccgctcccgc ttctgcgccg acttcgggtt
     1441 ccggggcgtc tacaacttcg accgccccgc ccgccgaccc caggcccagg tccccaccga
     1501 gcaggcgcgc cagcaggacc gccgccgcgc cctccggatc ggcgccgcct cggccgccat
     1561 cggggtcggg gtcgcggtgg tcgccttgct cgcctgatcg aacatcattg ggcccgtccg
     1621 tcaccatcct cccggctccg aaaggcagga tcgccatgga cgtggtcgtc gtccccctgg
     1681 tctggcagga cgcggcccca tgagcctgct ggtccgcgac aacaccacca tccgcctcca
     1741 agaccactgg ccgacccagg acgacctcgg cctgctggtc ctgatgcccg gcggcgaagc
     1801 cgggatcctc accagctggt ggcacgcccc cgaccattcc gagtggcgct ggcagctgga
     1861 gctctccggc caccgctaac ctcccgaagg acacgacgat ggttcccaca gccaccaccg
     1921 ccctcgccac cacccgtgtc ggcgatctcc accgccaagc ccaggtcagc ccctggacac
     1981 tcctcgtgat ggaccgccgc caggacgggg accatactca tccccatgac aacgcccacc
     2041 ccaaggtgcc cgacctgcgg cggcctgctc gcccacgacc cgcattcgct cctgtcacct
     2101 ggcgagcggc tcacaccatg gatgatccat gacctgccga ggatcgcctg gatcgtggag
     2161 caccgggcag cagagctgac cgacgaggag cgccagatcg ttcgcgacgc caccacccgg
     2221 atgctgcacg ccctggagca cgacaccgtc tgagctggtc gccgtcaccg gttgctcaac
     2281 tccgccgagg gggttgtcgg tggagatgat gggactcgaa cccacgaccc ctgcctgcaa
     2341 agccggatcc ggcaggatcg ccacctccga cggcagtgaa cgctgccggt caagaggggc
     2401 agagtgtgtc cgttgttgtc ccctggagcc cgggtcgtcg ccagcctcgt tggcaagtcg
     2461 cggggaggcg cgcggcagca ggacaccaga ggactcagcg gtccatgcca tgctggtcga
     2521 cgtgagttga gcggcgtagc gttcgctcat ggcggccaac cgcgagcggg tggcgctcgc
     2581 ctccttcatc atcggctcgg tgctggccgg cggcaatgcg gtgggtgtcc ggttcagcaa
     2641 ccgcgagctg ggcccgctgt ggggcgccgg cctgcgcttc ctggtggccg ccgg
//
LOCUS       DASSWR010000024         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_170584,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000024 DASSWR010000000
VERSION     DASSWR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170584"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1160..1549)
                     /locus_tag="VFO47_00490"
     CDS             complement(1160..1549)
                     /locus_tag="VFO47_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8787206.1"
                     /translation="MASIGLASATANLVGKGLLRRARPDRAGVNVPTGRMVRMPGSTS
                     FPSGHSASAFAFATAVGDELPLAWLPLHALAAAITYSRVHTGVHYPGDVLVGSLMGTG
                     AAVAVRYSGRILMRRSASPSRPGSAAP"
     gene            complement(1793..>2237)
                     /locus_tag="VFO47_00495"
     CDS             complement(1793..>2237)
                     /locus_tag="VFO47_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET8787207.1"
                     /translation="GPRLVARAGNVTIEGPQAVLVSNNVYGMGDIAGLGRRARLDRGN
                     LGVFAVSVSSAVQAAGLVRGTASRGLTRLVGREVTVDADAAQVPVGVDGEALLLDTPV
                     RCTIRPAALRVRVPRDRPGVPDPKPIMDWKRLRQMALTGPGTGRG"
BASE COUNT          336 a          786 c          813 g          302 t
ORIGIN      
        1 tcgccgagtc gctcccatcg ccggcaccgc aaccagtgag ccctaggacc aggctcagca
       61 cgagcccggc caggagccat gccgtggttg cccggcggcc agggatgccg ggtttcaccc
      121 accggtccct ggtgggggac aggatccgcc gcgacagcat ccaagacgtc ggcatcgatg
      181 accaccctcc gggtcagcca gggcaagtgg gggagtgcgc ccggcatgcg acgccggcca
      241 ttctcggccg atggtccgtc cgcaacatca tccaaagcgg gtgagaacgc ggcctccaga
      301 acctgcctcc gggtacacgg cccaggcgga acggacggcc acgtgcgccc atgtggggtc
      361 cggggccatt cggcgggttg ctggcgggcg gggctggggg agaccgccag cctgcgtgtc
      421 ccgccctacc agggcgtgct gtcgtgttgt tggtgatcct tgttgggcca ttggatacgc
      481 ggtgttggcc atgctcacca gggcgtccag caggccccgg tgattgctcg ggcagggatc
      541 aggccgtcca gagcgtggac gagctggagg agtctgtctc tgagtgcgga gaccggcctg
      601 ggcgagctga ggtgtgggtt ggactgccat cgtcctagga ggtggttggc cagtggccag
      661 gtcgaccgcg tggccgacca ggagaggctg ggtgcgcgcg aggatgtccc gccggtcggg
      721 agcctttgcg cagcgaggtt cgcccctcct gtggcaggct gcgcggcacg tcacttccac
      781 cattgctgct cagaacccgc ctaggaccgt gcgcctaccc agacgccggc ctcggcgtgg
      841 gcgtccagca gcactcgcca gctgcgatcc ccaaaccgcg acgcctgttc agtgatccca
      901 gcatgtcgct gaacaacacg gtggccagca tgcggtcggg cgcagccggt ggccggaccc
      961 ggtgaggaac tcggccactc gtccaggacc tgcgaggcgt gctacatacc ttgccctacg
     1021 acggcaagga caggaggtcg ctgtcccggc cgacccggac atcgtgacac cgcccgcctg
     1081 aagtggttgc tagggctacg cacgctgctc gactacaggg ccgcctggct ggcacgccac
     1141 ctggccgccg ggctgctgct cacggcgctg ctgaccccgg ccgagatggg ctggcgctcc
     1201 ggcgcatcag gatgcggccg ctgtagcgga cggcgacggc ggcgccggtc cccatgagcg
     1261 agccgaccag cacatcaccc ggatagtgca cgccggtgtg gaccctggag taggtgatgg
     1321 cggcggccaa ggcgtgcagc ggcagccacg ccaggggcag ctcgtcgccg accgccgtcg
     1381 cgaaggcaaa ggccgaggcg gagtgaccag aagggaacga cgtcgagccg ggcatcctga
     1441 ccatgcgccc tgtcggcacg ttcaccccgg cccggtcggg acgggcccgc ctgagcagcc
     1501 ccttgcccac gaggttggcc gtcgccgagg cgagcccgat gcttgccacc cccagcagcg
     1561 cggccctccg tggccggccc ggcttgagca tgagcgccgc ggcgatggcg aaccacagct
     1621 tggagtggtc ggcggccgac gacagccggc tcaggacgag gtcgaggctg ggcgacggcg
     1681 tggccgcgat cgcccgatag acggcctgac ggcctggtcg atgtggtcca gctcaccgag
     1741 agcgccgccg tagatggtcg cgggaggcgt cggccgcccg cgaccggcgc cgtcacccac
     1801 ggccggtgcc cggcccggtg agggccatct ggcgcaggcg tttccagtcc atgatgggct
     1861 tgggatcggg caccccagga cggtcacgag gcacccgcac ccgcagggca gccggccgga
     1921 tcgtgcagcg aacgggcgtg tccagcaaca gcgcctcacc gtcgaccccg accgggacct
     1981 gggcggcgtc ggcgtcgacg gtgacctcgc gcccgaccag tctggtcagc ccgcgggacg
     2041 cagtcccgcg gacaaggccg gccgcctgga ccgcgctgct gacggagacg gcgaacacgc
     2101 ccaggttgcc acggtccagc cgggcccggc gccccagccc ggcgatgtcg cccatcccat
     2161 agacattgtt ggagaccagc accgcctggg gcccctcgat ggtcacgttc ccggccctgg
     2221 cgaccagccg cgggccc
//
LOCUS       DASSWR010000025         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_29172,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000025 DASSWR010000000
VERSION     DASSWR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29172"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1583
                     /locus_tag="VFO47_00500"
     CDS             105..1583
                     /locus_tag="VFO47_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787208.1"
                     /translation="MPARLRWYVGSLTVAAGFVLAFLLTRLDLDRAREVAGAIAVFAL
                     FMIAGELVILRINAQNRLKEVSITSTFAYGLVPLAGTAMAVLVFLLGSVVSDLGRRKG
                     AIKTLFNAAQYVLALAAGGAVYHALGGGYEITTATLPALAAGGAAFMLVNHLLVSTVV
                     SLDQGIPVLQGLRRDDLRLELGSSAMVLALAPVAVVVAERSLLLVPALVVPLAAVFLA
                     SAGEIRARAQQAVAEEATERQRLLTEREHEVVRRLQETDRLKADLIATVSHELRSPLT
                     TIVGVFGILRVRRQRLSASERDELVRMGIHQSERLHRMIEQLLLAARFEQAEGVASLG
                     ASRTELDAIDLVHQAGAEARARHTGRRIAIETNGSLPVRVAQDAVVQVLANLLDNACK
                     YSPDTEPVRLSGTREGAEAVLAVEDAGPGIPEAERQRIFDRFTQLERHMQRRSGGVGL
                     GLYIARQLARSQDGDLLLVDPASGRGARFELHLPLLPEATGS"
     gene            1696..>2231
                     /locus_tag="VFO47_00505"
     CDS             1696..>2231
                     /locus_tag="VFO47_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787209.1"
                     /translation="MTQYGTDPVYRDPVAEPPAGSTSDQVKDQVRDKAQVAQDKARGA
                     FGQARGRLSGQVDQRSTQAGERIAGTASDVRSIAEELRSQGKDAPANLAEQVAGQADR
                     VGDYLKGASGDRILRDVEDFARRQPMLVAAGALALGFAASLFLKASSGRRYEAAYQPR
                     HQRDGDGAYGAYDDRTDVL"
BASE COUNT          311 a          840 c          787 g          293 t
ORIGIN      
        1 gatggtgata gttcaaaagg gctgatacat acgtaccccg ggcggatcag ctcggggtac
       61 gctagtcaga agaatcggtc gtcacctcgc cggggagggg caggatgccg gccagactgc
      121 ggtggtacgt cggcagcctc accgtggccg ccgggttcgt gctcgccttc ctgctgaccc
      181 ggctcgacct cgaccgcgcc cgcgaggtcg ccggggccat cgccgtgttc gccctcttca
      241 tgatcgccgg cgagctggtc atcctccgga tcaacgccca gaaccggctc aaggaggtgt
      301 cgatcacctc caccttcgcc tacgggctgg tccccctggc cgggaccgcg atggccgtgc
      361 tggtgttcct cctcggctcg gtcgtctccg acctgggccg gcgcaagggc gccatcaaga
      421 cgctgttcaa cgcggcccag tacgtgctcg ccctggccgc cggcggggcc gtctaccacg
      481 ccctgggcgg cggctacgag atcaccaccg cgaccctgcc cgcgctggcg gccggcgggg
      541 ccgccttcat gctggtcaac cacctgctgg tcagcaccgt ggtcagcctc gaccagggca
      601 tccccgtgct ccaggggctg cggcgcgacg acctccgcct ggagctgggc agctcggcca
      661 tggtgctggc gctggccccg gtggcggtgg tggtcgccga gcgcagcctg ctgctggtcc
      721 cggccctggt ggtgcccctg gcggccgtgt tcctggcctc ggcgggggag atccgggcca
      781 gggcccagca ggccgtcgcc gaggaggcga ccgagcgcca gcgcctgctc accgagcgcg
      841 agcacgaggt cgtccggcgc ctgcaggaga ccgaccggct caaggccgac ctgatcgcga
      901 ccgtctccca cgagctgcgc agccctctga ccacgatcgt cggcgtattc gggatcctgc
      961 gggtccggcg ccagcggctg agcgcgtccg agcgcgacga gctggtccgg atgggcatcc
     1021 accagagcga gcggctgcac cgcatgatcg agcagctgct gctggccgcc cgcttcgagc
     1081 aggccgaggg ggtggcctcc ctgggcgcgt ccaggaccga gctggacgcc atcgacctgg
     1141 tccaccaggc cggggccgag gccagggcgc gccacaccgg ccgccgcatc gccatcgaga
     1201 ccaacgggtc cctgccggtc cgggtcgccc aggacgcggt cgtgcaggtg ctggccaacc
     1261 tgctcgacaa cgcctgcaag tactcgcccg acaccgagcc ggtacggctg tcggggaccc
     1321 gcgagggcgc cgaggccgtg ctcgccgtcg aggacgccgg ccccggcatc cccgaggccg
     1381 agcgccagcg catcttcgac cgcttcaccc agctcgagcg ccacatgcag cgccgctccg
     1441 gcggcgtcgg cctggggctg tacatcgccc gccagctcgc ccgcagccag gacggcgacc
     1501 tcctgctggt cgacccggcc agcggccgcg gcgcccgctt cgagctccac cttcccctgc
     1561 tcccggaggc caccggatcc tgacggcggc acatccgtcg ttttggagct cgatgccggg
     1621 atagacagac gcgtgcgcgc gcttgaacca tcaagccgat cgaggcatgc ggcgaggcat
     1681 gcaggaggag atgtcatgac ccagtacggc accgacccgg tgtaccggga cccggtcgcg
     1741 gaaccgccgg ccggttccac cagcgaccag gtgaaggacc aggtccgcga caaggcccag
     1801 gtggcccagg acaaggcgcg gggcgcgttc gggcaggcca ggggccggct cagcggccag
     1861 gtcgaccagc gctcgaccca ggccggggag cgcatcgccg ggaccgcctc ggacgtgcgc
     1921 tcgatcgccg aggagctgcg cagccagggc aaggacgccc cggcgaacct ggccgagcag
     1981 gtcgccggcc aggccgaccg cgtgggcgac tacctcaagg gcgccagcgg cgaccggatc
     2041 ctgcgcgacg tcgaggactt tgcccgccgc cagcccatgc tggtcgcggc cggcgccctc
     2101 gccctcggct tcgccgcctc cctgttcctg aaggcgtcca gcggccgccg ctacgaggcc
     2161 gcctaccagc cgcgtcacca gcgcgacggc gacggcgcct acggcgccta cgacgaccgg
     2221 accgacgtcc t
//
LOCUS       DASSWR010000026         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_481066,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000026 DASSWR010000000
VERSION     DASSWR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481066"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1267
                     /locus_tag="VFO47_00510"
     CDS             119..1267
                     /locus_tag="VFO47_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET8787210.1"
                     /translation="MSTAGAYERSVDGEGAPVSEPLVKITGVKKYFPITQGVIFQREV
                     GRVHAVDGVDLEVFPGETLGIVGETGCGKSTLARLISRLLPVTEGTIEFDGQDITHLK
                     GNGLRSLRREIQMIFQDPYSSLNPRKRVGAIIGDPFDIHGLAEGKERKQRVQELMEVV
                     GLNPEHYNRFPAEFSGGQRQRIGVARALALRPKLIICDEPVSALDVSIQAQVVNLLED
                     LQEEFHLTYVFIAHDLSVVRHVSDRVAVMYLGKVVELADARRLYAEPKHPYSGALLSA
                     VPVPDPDKVHERKRVILLGDVPSPIDPPSGCRFHPRCPKAQQRCVDEVPPLETKASGD
                     LAACHFPLEPGEDLAAARPGIEQDAAAVAVAGATGGTELPPDEPPHPA"
     gene            complement(1284..2249)
                     /locus_tag="VFO47_00515"
     CDS             complement(1284..2249)
                     /locus_tag="VFO47_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005480028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787211.1"
                     /translation="MWRYIVRRLLWVVLVVLVITAFTFVIFFVMPPTDPAVQFAGKNP
                     TPEQIAEVRRQFGLDKPVWQQYVLFLKNIFTGDEYGWPGLGFSFVTRSPVKDLLFDRI
                     WVTIQLAVGAALVWLCIGIPIGIVSAIKRGSLIDRASMLFALFFVSAPVFWLGLMALW
                     LFWYKLGISPGTGYVPIGESFSGWLSHMIMPWVVLALLYAAFYARMTRGNLIETMGQD
                     YIRTARVKGLPEWKVILKHGVRASLTPVVTLIGLDLGALLGGAVITETVFNLQGIGQW
                     AVNSVFQGDLPAVLAVTVIVALAVTLMNLVVDVVYAYLDPRVRYT"
     gene            complement(2330..>3323)
                     /locus_tag="VFO47_00520"
     CDS             complement(2330..>3323)
                     /locus_tag="VFO47_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8787212.1"
                     /translation="FNKVDTGALDTSFTDDAPPTLLQRYSADQAKKNLIHVNEGDRTW
                     YITMNWATPPFDDINVRKAVNQVMDKAGMLQAWGGGIAGQIATHIMPPNVLNGQLTAE
                     YNPYASEGNRGDEAKAKELMKQSKYDTNKDGLCDGAKCTGLVMINRNVAPWTAMEPVV
                     VESLAKIGIKVTARELASSAAYTTIQTVKNMIPIALNAGWGKDYPDPYSFAAPLFTKE
                     ALIPTGNTNYALVGLDQAKAEELGVDIPSGAQIPNVDADVNRCQEIVEVEARTTCFVD
                     LDKKLMEQAVPWVPYLWNSVIVITNPSVTKYEFDQFSTEMSITEIAVSNKETLS"
BASE COUNT          529 a         1108 c         1155 g          531 t
ORIGIN      
        1 gggaagagcc gaagctggtg ccggccgacc cggccgggca gccttcgcac ctgtccgcct
       61 gccacctgtc cctggaggag aaggagcgga tctggcagca ggaggtggca cccacccgat
      121 gagcacggcg ggcgcatacg agaggtcggt ggacggggag ggtgcgccag tgagcgagcc
      181 gctggtcaag atcaccgggg tcaagaagta cttcccgatc acccagggag tcatcttcca
      241 gcgcgaggtc ggccgcgtgc acgccgtcga cggcgtcgac cttgaggtct tcccgggcga
      301 gacgctcggc atagtggggg agacgggctg cggcaagtcg accctggcca ggctgatctc
      361 gcggctgctg ccggtcaccg agggcacgat cgagttcgac ggccaggaca tcacccatct
      421 caagggcaac gggctgcgat cgctccggcg cgagatccag atgatcttcc aggacccgta
      481 ctcgtcgctg aacccgcgca agcgggtcgg ggcgatcatc ggcgacccgt tcgacatcca
      541 cgggctggcc gagggcaagg agcgcaagca gcgggtccag gagctgatgg aggtcgtcgg
      601 cctcaacccc gagcactaca accggttccc ggccgagttc tccggtggcc agcgccagcg
      661 catcggggtg gcccgcgccc tggccctgcg gcccaagctg atcatctgcg acgagccggt
      721 gtcggccctc gacgtctcca tccaggccca ggtggtcaac ctgctcgagg acctgcagga
      781 ggagttccac ctcacctacg tgttcatcgc ccacgacctg tcggtggtgc gccacgtgtc
      841 ggaccgggtg gcggtgatgt acctcggcaa ggtggtcgag ctggccgacg cccgccgcct
      901 gtacgccgag cccaagcacc cctacagcgg ggcgctgctg tcggcggtgc ccgtgcccga
      961 cccggacaag gtccacgagc gcaagcgggt catcctcctc ggcgacgtgc cgtcgccgat
     1021 cgacccgccg tcgggctgcc ggttccaccc ccgctgcccc aaggcccagc agcgctgcgt
     1081 cgacgaggtg ccgccgctgg agaccaaggc ctccggcgac cttgccgcct gccatttccc
     1141 gctcgagccg ggcgaggacc tggccgcggc gcggccgggc atcgagcagg acgcggccgc
     1201 ggtcgccgtg gccggggcca ccggagggac cgagctgccg cccgacgagc ccccgcaccc
     1261 cgcctagcga acctgccgga gcgctaggtg taccggaccc gcgggtccag gtaggcgtag
     1321 accacgtcca cgaccaggtt catcagggtc acggcgaggg ccacgatcac ggtcacggcc
     1381 agcacggccg ggagatcgcc ctggaacacc gagttcaccg cccactggcc gatgccctgg
     1441 aggttgaaca ccgtctcggt gatcaccgcc ccgccgagca gggcgcccag gtcgagcccg
     1501 atcagggtca ccaccggggt caggctggcc cgcacgccgt gcttgaggat gaccttccat
     1561 tcgggcaggc ccttgaccct ggccgtccgg atgtagtcct ggcccatcgt ctcgatcagg
     1621 ttgccccggg tcatgcgggc gtagaaggcc gcgtacagca gggccagcac cacccagggc
     1681 atgatcatgt ggctcagcca gcccgagaag ctctcaccta tcgggacata gccggtgccc
     1741 ggcgagatcc ccagcttgta ccagaacagc cacagcgcca tcaggccgag ccagaacacc
     1801 ggggccgaca cgaagaacag tgcgaacagc atcgatgcgc ggtcgatcag ggagccgcgc
     1861 ttgatcgccg agacgatccc gatggggatg ccgatgcaca gccagaccag ggccgcgccc
     1921 acggcgagct ggatggtgac ccagatccgg tcgaagagca ggtccttcac cggtgagcgg
     1981 gtcacgaagg agaagccgag ccccggccag ccgtactcgt cgccggtgaa gatgttcttc
     2041 aggaacagca cgtactgctg ccagaccggc ttgtccaggc cgaactggcg gcgcacctcg
     2101 gcgatctgct ccggggtggg gttcttgccg gcgaactgga cggccgggtc ggtcggtggc
     2161 atcacgaaga agatgacgaa ggtgaaggcc gtgatgacca ggaccaccag gacgacccac
     2221 aggagccgtc ggacgatgta gcgccacatg tgctgtcacc cggccgttct tgcgtctcgg
     2281 gggaggggcg ggggccgccc cgggcggccc ccgccgtctc accgtgccac taggagaggg
     2341 tctccttgtt gctcaccgcg atctcggtga tggacatctc ggtcgagaac tggtcgaact
     2401 cgtacttggt cacgctcggg ttggtgatga cgatcaccga gttccacagg tagggcaccc
     2461 aggggacggc ctgctccatc agcttcttgt ccaggtcgac gaagcaggtg gtccgggcct
     2521 cgacctcgac gatctcctgg cagcggttca cgtcggcgtc cacgttcggg atctgggccc
     2581 cggaggggat gtcgacgccc agctcctccg ccttcgcctg gtcgagcccg accagcgcgt
     2641 agttggtgtt gcccgtcggg atgagcgcct ccttggtgaa cagcggcgcc gcgaacgagt
     2701 aggggtcggg gtagtccttg ccccagccgg cgttcagcgc gatcgggatc atgttcttga
     2761 cggtctggat ggtggtgtag gcggcgctgg aggcgagctc gcgggcggtc accttgatgc
     2821 cgatcttggc caggctctcg acgaccaccg gctccatggc ggtccagggg gccacgttgc
     2881 ggttgatcat caccaggccg gtgcacttgg cgccgtcgca gagaccgtcc ttgttggtgt
     2941 cgtacttcga ctgcttcatc aactccttgg ccttggcctc gtcaccccgg ttgccctccg
     3001 aggcgtaggg gttgtactcg gccgtgagct gaccgttgag gacgttcggc ggcatgatgt
     3061 gggtggcgat ctggccggcg atgccgccgc cccaggcctg cagcatcccc gccttgtcca
     3121 tcacctggtt gacggccttg cgcacgttga tgtcgtcgaa cgggggcgtg gcccagttca
     3181 tcgtgatgta ccaggtgcgg tcgccttcgt tgacgtggat caggttcttc ttggcctggt
     3241 cggccgagta ccgctgcagc agggtcggcg gcgcgtcgtc ggtgaacgag gtgtccaggg
     3301 cgccggtgtc caccttgttg aag
//
LOCUS       DASSWR010000027         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_848774,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000027 DASSWR010000000
VERSION     DASSWR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848774"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..735)
                     /locus_tag="VFO47_00525"
     CDS             complement(70..735)
                     /locus_tag="VFO47_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8787213.1"
                     /translation="MIKVLIVDDDFMVARIHSGFVAQMPGFEVAGVARTGAQALAEAR
                     RIQPDLVLLDIHLPDIYGLELLGQLRAEVPELDVLVISAAREAETVRRALRGGIVHYL
                     MKPFTQEDLHQRLEHYRRTYRSLGSAATAEQSDVDAVFGMAGADRPLPKGCAAETMER
                     VQGILREAEADLSALEVGEMLGLSRVSARRYLEYLNEVGQAQVNLRYGGGRPERRYSR
                     RGA"
     gene            complement(732..2306)
                     /locus_tag="VFO47_00530"
     CDS             complement(732..2306)
                     /locus_tag="VFO47_00530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET8787214.1"
                     /translation="MSLAGQFLGLQLLILLAVMAGVLAMSLAQATQTFERVESRRSLA
                     AAENLAATPLVRALLPEARPRFGSALPSITESVRSVSGSSEAVLVNAEGTVVTASNPA
                     LLGEKFPLGESDVMEGRAWTGSTQRGGIKMLEAHVPVLDDGGRLVGFAAMGRSYPSLL
                     ERLLEATPNLLVYLGVSVALGTLGSLLLSRRVKRQTLGLEPAEITGLVEHREAILHGV
                     KEGVVAIDLRERITLANDAARRLLGWPEDCEGRTLADLSIDAALREVLTMKQAEADRL
                     VLVGERMVVFNRRPMEARGRLVGSVTTLRDRTELTTLEKELGATRMTTETLRAQTHEF
                     ANQLHTISGLIQLEEYDEVLSYVNGVSFSRTRLLDDVTHRVADPTVAALLIAKSALAS
                     ERGVGIDLDPASQLGRVDEALSRDLTTVVGNLVDNAIDAVAGRPDARVKVRLAEEGQT
                     VVVTVHDSGEGVDESNVAKIFVQGYSTKTSSSEAGRGFGLALTRLVCLRRGGDVTVAN
                     DQGAVFTARLGKRVQP"
     gene            2565..>3171
                     /locus_tag="VFO47_00535"
     CDS             2565..>3171
                     /locus_tag="VFO47_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8787215.1"
                     /translation="MGRTNILKLAAVAAGIMLAATGCGVTGNDAGASGSAAESGPVTG
                     LRIMVPNSPGGGYDTTARVGAKVMEEAEIAKSVEVFNLAGAGGTVGLARIVNEKGNND
                     LGMLMGLGVVGASYTNDTDARLTDTTPIARLIQEPGAIMVSKDSPYKTIEDLVAAWKK
                     DPSKLAVGGGSSPGGPDHLLPMQLAQAVGIDAKKVNYVSYDG"
BASE COUNT          490 a         1131 c         1098 g          452 t
ORIGIN      
        1 cgcgaggcgc accagaagct gcagaacggg tccttcggcg aggacgactg gctgtagcgg
       61 ggccgcggct caggccccgc gccggctgta tctgcgctcg ggacgcccgc cgccatagcg
      121 caggttcacc tgcgcctggc cgacctcgtt gaggtactcc aggtagcgcc gcgcgctgac
      181 ccgggacagg cccagcatct cgccgacctc cagcgcggag agatccgcct ccgcctcgcg
      241 caggatgccc tgcacccgct ccatggtctc cgccgcgcag cccttgggca gcggcctgtc
      301 ggccccggcc atgccgaaga ccgcatcgac gtcggactgc tcggcggtgg cggccgaacc
      361 cagcgaacgg taggtgcgcc ggtagtgctc caggcgctgg tgcaggtcct cctgggtgaa
      421 cggcttcatc aggtagtgca cgatgccccc gcgcagcgcc cggcgcaccg tctcggcctc
      481 gcgggcggcg ctgatcacca gcacgtcgag ctccgggacc tccgcgcgca actggcccag
      541 cagctccagc ccgtagatgt cgggcaggtg gatgtccagc agcaccagat cgggctggat
      601 ccggcgggcc tcggccaggg cctgggcccc ggtgcgggcg acccccgcga cctcgaagcc
      661 gggcatctgc gccacgaagc ccgaatggat ccgcgcgacc atgaagtcgt cgtcgacgat
      721 gagcaccttg atcatggctg aacccttttc cccaggcggg cggtgaagac cgccccctgg
      781 tcgttggcga ccgtgacgtc gccgccgcgg cgcaggcaca ccaggcgggt cagcgccagg
      841 ccgaagccgc gcccggcctc ggaggaggag gtcttggtgc tgtagccctg cacgaagatc
      901 ttagcaacgt tcgactcgtc gacgccctcg cccgagtcgt gcaccgtgac caccaccgtc
      961 tggccctcct cggccagccg gaccttgacc cgcgcgtcgg gtcgccctgc caccgcgtcg
     1021 atggcgttgt ccaccaggtt gccgaccacc gtggtcaggt cccgcgagag ggcctcgtcc
     1081 acccggccca gctgcgaggc aggatccagg tcgatgccca cgccccgctc cgaggccagc
     1141 gctgacttgg caatgagcag cgcggccacc gtggggtctg ccacgcggtg ggtgacgtcg
     1201 tccagcagcc gggtgcggct gaagctcacc ccgttgacgt aggagagcac ctcgtcgtat
     1261 tcctctagct ggatcagccc ggagatggtg tgcagctggt tggcgaactc gtgggtctgg
     1321 gcgcgcagcg tctcggtggt catgcgcgtg gcccccagtt ccttttcaag ggtcgtcagt
     1381 tcggtgcggt cgcgcagtgt ggtcaccgag ccgaccagcc ggccgcgggc ctccatgggg
     1441 cggcggttga acaccaccat gcgctcgccg accagcacca gccggtccgc ctcggcctgc
     1501 ttcatggtca gcacctcgcg cagggccgcg tcgatggaca ggtccgccag ggtccggccc
     1561 tcgcagtcct cgggccagcc cagcagccgg cgggcggcgt cgttggccag ggtgatgcgt
     1621 tcgcgcaggt cgatggccac cacgccctcc ttgacgccgt gcaggatggc ctcgcggtgt
     1681 tcgaccagcc cggtgatttc cgccggctcc aggcccaggg tctggcgctt gacgcggcgg
     1741 gagagcagga gcgagccgag cgtgcccagg gccaccgaga cccccaggta gaccagcagg
     1801 ttgggggtgg cctccagcag gcgttcgagc agcgaggggt agctgcgccc catggcggcg
     1861 aagcccacga gccgcccgcc gtcatcgagc accgggacgt gcgcctcgag catcttgatg
     1921 ccgccgcgct gcgtggaccc cgtccaggcg cggccctcca tgacgtcgga ttcgcccagc
     1981 gggaacttct ccccgagcag ggcgggattc gaggcggtca ccacggtccc ctcggcgttg
     2041 accagcaccg cctcgctgct gccggagacc gagcgcaccg actcggtgat cgagggcagg
     2101 gccgagccga agcggggcct ggcctcgggc agcagggcgc gcaccagcgg ggtcgctgcg
     2161 aggttttcgg cagccgccag cgagcgccgg gactcgacgc gttcaaaggt ctgggtggcc
     2221 tgggccaggg acatggcaag gaccccggcc atgacggcga gcaggatcag caattgcagt
     2281 cccaggaact ggcccgcgag gctcagccgg gcggtgcggc cccaacgcgg ggcgggggag
     2341 tggccggaca ccggcggtcc tttccgtgcc atgacgcacc cggcgccaat cgcccggtcc
     2401 ggcatatgcc atcggcgtga actctaccga catcgggcat ccgcgccgaa tgtgaccgtg
     2461 aacactatgt acacaacttt ctttgcgacc acaagaattg cccggccctg ctggcggcct
     2521 agtgtggtgc acatcattca ctcgcatttg agatgggaat caccatggga cgaaccaata
     2581 tcttgaagct ggcagcggtt gcggccggca tcatgctcgc cgccaccggc tgcggggtca
     2641 ccggcaatga cgccggcgcc tccggcagcg cggccgaatc cggcccggtg accggcctgc
     2701 gcatcatggt ccccaacagc cccggcggcg gctacgacac cacggcccgc gtcggcgcca
     2761 aggtcatgga ggaagcggag atcgccaagt cggtggaggt cttcaacctt gccggcgccg
     2821 gcggcaccgt ggggctggcc cgcatcgtca acgaaaaggg caacaacgac ctgggcatgc
     2881 tcatgggcct gggcgtggtg ggcgcgagct acaccaacga caccgacgcc cggctgaccg
     2941 acaccacccc gatcgcccgg ctgatccagg agccgggggc catcatggtc tcgaaggact
     3001 ccccgtacaa gaccatcgag gacctcgtcg cggcctggaa gaaggatccg tccaagctgg
     3061 ccgtcggcgg cggctcctcg ccgggcggcc ccgaccacct gctgccgatg cagctggccc
     3121 aggccgtggg catcgacgcg aaaaaggtca actacgtctc ctacgacggc g
//
LOCUS       DASSWR010000028         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_707442,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000028 DASSWR010000000
VERSION     DASSWR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707442"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1097
                     /locus_tag="VFO47_00540"
     CDS             87..1097
                     /locus_tag="VFO47_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005568777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET8787216.1"
                     /translation="MDVAFVLAVVAILAFAFTNGFHDAANAIATLVATRAARPGPAIV
                     LAAACNMLGPLLLGAAVADTIASIVEVTPEQTIPLVGAALTAAVTWNIVTWWKGLPSS
                     SSHALVGGLVGAAIVEAGVSAVNWGGVGGGHPEGVLGALAALAISPVLGFGGAWLMER
                     AALRALRRATVHVYRPVLQAQWVTSGWLGFSHGANDAQKAVGVLAVLLLANGSTTSLS
                     APVWATMSCAAALTVGTALGGWRIVKTIGRRIFRIRPLDGLVSQTSSAAVILAASVIG
                     APASTTQVVSSSVVGVGVGRRRYRHVGWEVVGSILLAWVTTFPAAALLAALFVPLWRL
                     IP"
     gene            1094..1717
                     /locus_tag="VFO47_00545"
     CDS             1094..1717
                     /locus_tag="VFO47_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET8787217.1"
                     /translation="MKRSWFLPENPDVLGLLCHQAKVTQEGMEALRAWANAEPDAAKQ
                     VREWEHKADDAKKELRRALTIAFTTPVDAEDIYVMSERLDAVINGAKDLVREAEVMRI
                     EPDEATADMAALLADGVRQLTLAFERLSQNRHGDGDETATAAADAAVKTQRQVERVYR
                     KAASALLTVDDLREVIGRRELYRRFSRVSETLIEAADRVWYATVKEG"
     gene            complement(1745..2194)
                     /locus_tag="VFO47_00550"
     CDS             complement(1745..2194)
                     /locus_tag="VFO47_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET8787218.1"
                     /translation="MRFRTTIVQSGKNTTAIQVPEEVMEALGAGKRPAVTVTVNGYRY
                     RSTVASMGGVAMVSLSAEHRAGAGVAGGDEVEVDLELDTAPREVEVPADLAAALDAEP
                     AARSTFDGLSYSNRSWHVLQVTGAKTDETRRRRIARSVDLLKQGRAR"
BASE COUNT          299 a          868 c          857 g          338 t
ORIGIN      
        1 agaaggcacc tcggcttcca cacctcccgc gagttggcca caatccagac gtcggcgagc
       61 ggctggcggg cgggagggct gggagcgtgg atgtcgcgtt cgtgctggcg gtcgtcgcca
      121 tcctggcctt cgccttcacc aacggcttcc acgacgcggc caacgccatc gccaccctgg
      181 tcgccaccag ggccgcccgc ccggggccgg ccatcgtcct ggccgcggcc tgcaacatgc
      241 tcggcccgct gctgctgggc gcggccgtgg ccgacaccat tgccagcatc gtcgaggtca
      301 ccccggagca gacgatcccg ctggtcgggg ccgccttgac cgccgccgtg acctggaaca
      361 tcgtgacctg gtggaagggg ctgccctcca gctccagcca cgcgctggtc ggagggctgg
      421 tcggggcggc gatagtggag gcgggcgtct cggcggtcaa ctggggcggc gtcggcggcg
      481 ggcacccgga gggcgtgctc ggggccttgg cggcgctggc catctcgccg gtgctcgggt
      541 tcggcggggc ctggctgatg gagcgggccg ccctgcgcgc cctgcgccgg gccaccgtcc
      601 acgtgtaccg gccggtgctc caggcccagt gggtgacctc gggatggctg gggttcagcc
      661 acggcgccaa cgacgcccag aaggcggtgg gcgtgctggc cgtgctgctg ctggccaacg
      721 ggtcgaccac gtcgctgtcg gcgccggtct gggcgacgat gtcgtgcgcc gccgccctca
      781 ccgtcgggac ggcgctgggc gggtggcgga tcgtgaagac gatcggtcgc cgcatcttcc
      841 ggatccggcc gctcgacggc ctggtcagcc agacgagctc ggcggccgtg atcctggccg
      901 cctcggtgat cggcgctccg gccagcacca cccaggtggt ctcgtcctcg gtcgtcgggg
      961 tcggggtcgg ccgccgccgc taccgccacg tcggctggga ggtcgtgggc agcatcctgc
     1021 tcgcctgggt caccaccttt cccgccgccg ccctgctggc ggcgctcttc gtcccactgt
     1081 ggaggttgat cccgtgaaac ggagctggtt cctgcccgag aaccccgacg tgctcggcct
     1141 gctgtgccac caggccaagg tcacccagga gggcatggag gcgctgcgcg cctgggccaa
     1201 cgccgagccc gatgcggcca agcaggtcag ggagtgggag cacaaggccg acgacgccaa
     1261 gaaggagctg cggcgggccc tcaccatcgc cttcaccacc ccggtcgacg ccgaggacat
     1321 ctacgtgatg tccgagcgcc tggacgccgt catcaacggc gccaaggacc tggtgcgcga
     1381 ggccgaggtc atgcggatcg agcccgacga ggcgacggcg gacatggcgg cgctgctggc
     1441 cgacggcgtc cgccagctca ccctcgcgtt cgagcgcctc agccagaacc ggcacggcga
     1501 cggcgacgag accgcgaccg cggccgccga cgccgccgtc aagacccagc gccaggtcga
     1561 gcgggtctac cgcaaggccg cctcggccct gctcaccgtc gacgacctcc gcgaggtcat
     1621 cggccgccgc gagctctacc gccgcttctc ccgggtcagc gagaccctga tcgaggcggc
     1681 cgaccgtgtc tggtacgcca ccgtcaaaga gggctgacca agaggttgcg cagcgcgccc
     1741 gtcgtcagcg ggcgcgcccc tgcttgagca ggtcgaccga cctggcgatc cggcgccggc
     1801 gggtctcgtc ggtcttggcc ccggtgacct ggaggacgtg ccaggacctg ttgctgtagg
     1861 acagcccgtc gaaggtgctc cgggcggccg gctcggcgtc gagcgcggcg gccaggtcgg
     1921 ccggcacctc cacctcccgg ggagcggtgt cgagctccag gtcgacctcg acctcgtccc
     1981 ccccggcgac cccggccccg gcgcggtgct cggcgctcag gctgaccatc gccaccccgc
     2041 ccatcgaggc cacggtgctc cggtaccggt acccgttcac ggtcaccgtg accgccggcc
     2101 gcttccccgc ccccagtgcc tccatcacct cctcgggcac ctggatcgct gtggtgttct
     2161 tgccgctctg cacgatcgtg gtccggaacc gcaggtcgtt ctcctctcta ccccccgggc
     2221 ccgtctcagc tacctgggat caccaacggg gccgtcgcgg tataggctcg cagaacggat
     2281 gggcaggggg aggtcgaggt ggagacgtcg ggcaaccggg ccgggcgtcg ggcggtgacg
     2341 ctccgggacg tggccgcggc gg
//
LOCUS       DASSWR010000029         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_311951,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000029 DASSWR010000000
VERSION     DASSWR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311951"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFO47_00555"
     CDS             <1..1210
                     /locus_tag="VFO47_00555"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET8787219.1"
                     /translation="VFLGHERRRGPLLNQAAMHRETAALLARLGDEGIAPTASVTSLR
                     PAQQQVVSMARALSHSVKLVILDEPSAVLDDVEVEALFKVVRRLTAEGVGIIWISHRL
                     GEVRQLGDRVTVLKEGRTVATELPPDSPVDTLIGHMVGGRMETLFPDRKPPREATVLQ
                     VRGLTRAPDVHDASFDLREGEILGLAGLVGSGRTELLRLIYGLDPAQSGEVEVGGRRL
                     PPGRPDLAIRAGLGFAPEERKSQGLWLDWSMVRNTSIADLARFQRGPFTDRAGERQEA
                     TRHLRSLKTRPDAPERPARQFSGGNQQKAVLARWLLRSCRVLLLDEPTRGVDVGARSE
                     IYRVIAELAGNGIGIVMVSSELAELLGFCHRVLVVREGHIVDELDGATSTEEQVLRSA
                     VPVHHTTKAG"
     gene            1215..2171
                     /locus_tag="VFO47_00560"
     CDS             1215..2171
                     /locus_tag="VFO47_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787220.1"
                     /translation="MAVDSPPRNALAGWRQVPVQELVLPVVIVALLVAGVIIKPDVFP
                     TSDNLLNVLTQASITGIIAIGMTFVIATGGIDLSVGSVLAAASIAGGLVFENGTWIFI
                     LGTIGFAVLLGAINGLAITWGRVVPFIATLAMLTIARGLALWMSDKTPISLSQLDILQ
                     RVGSGRVLGVPVPAVVFLAVTVAGWVLLNRTTFGRHTIAVGGNRSAARIAGIRSNRVI
                     FAVYVLTGLCVGISAILLSGRLASASPVVGNLVELDAIAAVVIGGTALAGGKATVTGT
                     FFGVITFALIFNLLNLLNMPAEIQGIVKGVLILAAVALQRRD"
     gene            2243..>3261
                     /locus_tag="VFO47_00565"
     CDS             2243..>3261
                     /locus_tag="VFO47_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8787221.1"
                     /translation="MRLPRMLVVMVALAALTLTACTNEVSDGGGEGATGGTSAPQAEG
                     VTGRPVKVGFSAPGADHGWLKAITDNARKSADELGVDLILSEGTNDTASQVSQVETLI
                     SQKPDVLVILPQEGGALTPIAQKAMQAGIPVVNVDREFSTPAAFRTWIGGDNYGIGEA
                     AANFIANELNCQGNVVEIQGVAGISVTEQRSKGFTDGIKRCNGGIKIVASQPADFLPD
                     KGLQVMETMLQGQQDIDAVYTHDDDMAMGAVQAIRNANRQDEMFITGAGGSKDAMNLI
                     AEGDLYRATFLYNPSMSASAISLAKLIAERKGLSELVEPEVPSRVELPATTVTKDNVA
                     SVQNLG"
BASE COUNT          495 a         1197 c         1095 g          474 t
ORIGIN      
        1 cgtgttcctc ggccacgagc ggcgccgggg tccgctgctg aaccaggcgg cgatgcaccg
       61 cgagaccgcg gcgctgctgg cccggctggg ggacgagggc atcgctccga cggcatcggt
      121 gacgtcgctg cggccggccc agcagcaggt cgtctcgatg gccagggcgc tgtcgcactc
      181 ggtgaagctg gtgatcctgg acgagccgtc ggcggtcctg gacgacgtcg aggtggaggc
      241 gctgttcaag gtggtcaggc ggctcaccgc cgagggagtc gggatcatct ggatctccca
      301 ccggctcggc gaggtgcgcc agctgggcga ccgggtgacg gtgctgaagg agggccggac
      361 ggtggcgacc gagctgccac ccgacagccc ggtcgacacc ctgatcgggc acatggtcgg
      421 cggacgcatg gagacgctgt tccccgaccg caagccgccc cgcgaggcga ccgtgctcca
      481 ggtgcgcggc ctgacccggg ccccggacgt ccacgacgcc tccttcgatc tgcgcgaggg
      541 cgagatcctc ggcctggccg ggctggtcgg ctcgggccgc accgagctgc tccgcctgat
      601 ctatgggcta gacccggccc agtccggcga ggtggaggtt ggcggtcgcc gcctcccgcc
      661 cggccggccc gacctggcca tccgggccgg cctggggttc gcgcccgagg agcgcaagtc
      721 ccagggacta tggctggact ggagcatggt ccgcaacacc tcgatcgccg acctggcccg
      781 gttccagcgc ggcccgttca ccgatcgcgc cggcgagcga caggaagcca cccgccacct
      841 gcggtcgctc aagacccggc ccgacgcgcc cgaacgcccg gcgcggcagt tctccggcgg
      901 caaccagcag aaggcggtcc tggcccgctg gctgctgcgc tcctgccgtg tcctgttgct
      961 ggacgagccg acccggggcg tcgacgtcgg cgcccgctcg gagatctatc gggtgatcgc
     1021 cgagctggcc ggcaacggga tcgggatcgt catggtctcc agcgagctgg ccgagctgct
     1081 cggcttctgt caccgcgtgc tcgtggtgcg tgagggccac atcgtcgacg agctcgacgg
     1141 cgccaccagc accgaggagc aggtcctgcg gtcagcggtc cctgttcacc acaccaccaa
     1201 ggcagggtga ggaaatggca gtcgacagcc ccccgcgcaa cgccctggcc ggctggcgcc
     1261 aggtccccgt ccaggagctg gtgctgccgg tggtgatcgt cgccctgctg gtcgccggcg
     1321 tgatcatcaa gcccgacgtc ttcccgacca gcgacaacct gctgaatgtg ctcacccagg
     1381 ccagcatcac cggcatcatc gccatcggca tgaccttcgt gatcgccacc ggcgggatcg
     1441 acctgtcggt cgggtcggtg ctggccgcgg ccagcatcgc cggcgggctg gtgttcgaga
     1501 acggcacctg gatattcata ctcggcacga tcggtttcgc cgtgctcctc ggggcgatca
     1561 acggcctggc catcacctgg ggccgggtcg tcccattcat cgcgaccctg gccatgctca
     1621 ccatcgcccg tggcctcgcg ctctggatga gcgacaagac ccccatctcc ctgagccagc
     1681 tggacatcct gcagcgggtg ggcagcggcc gggtgctcgg cgtgcccgtc ccggccgtgg
     1741 tcttcttggc cgtcacggtg gccggctggg tgctgctcaa ccgcaccacc ttcggccggc
     1801 acacgatcgc ggtcggcggc aaccgcagcg ccgcccggat cgccggcatc cgctccaacc
     1861 gggtcatctt cgccgtctac gtcctcaccg ggctgtgcgt ggggatctcg gccatcctgc
     1921 tgtcgggccg gctggccagc gcctccccgg tcgtcgggaa cctggtcgag ctggacgcga
     1981 tcgccgccgt ggtcatcggc gggaccgccc tggccggcgg caaggccacc gtgaccggca
     2041 ccttcttcgg cgtcatcacc ttcgcgctga tcttcaacct gctgaacctg ctcaacatgc
     2101 ccgccgagat ccaagggatc gtcaagggcg tgctcatcct cgccgccgtg gccctgcagc
     2161 gccgcgactg accacgtcgt ctgtctctgt ccggccgctc cggctcccag caggagccca
     2221 tcgcagaaag gaagggagca gcatgaggct cccccgaatg ctcgtcgtca tggtcgcgct
     2281 cgcggctctc acgctgaccg cctgcaccaa cgaggtctcc gacggcggcg gcgaaggcgc
     2341 caccgggggc accagcgccc cccaggccga gggcgtcacc ggccgtcccg tcaaggtcgg
     2401 cttcagcgct cccggcgccg accacggctg gctgaaggcg atcaccgaca acgcccgcaa
     2461 gtctgccgac gagctcggcg tggacctgat cctgtcggag ggcaccaacg acacggccag
     2521 ccaggtctcc caggtcgaga cgctcatcag ccagaagccg gacgtgctgg tcatcctccc
     2581 gcaagaaggc ggcgcgctca ccccgatcgc gcagaaggcc atgcaggccg gcatcccggt
     2641 ggtcaacgtc gaccgtgagt tctccacccc ggccgccttc cggacctgga tcggcggcga
     2701 caactacggc atcggcgagg ccgccgccaa cttcatcgcc aacgagctga actgccaggg
     2761 caacgtcgtg gagatccagg gcgtggccgg gatctcggtg accgagcagc gaagcaaggg
     2821 cttcaccgac ggcatcaagc gctgcaacgg cggcatcaag atcgtggcca gccagccggc
     2881 cgacttcctg cccgacaagg gcctccaggt gatggagacc atgctccagg gccagcagga
     2941 catcgacgcc gtctacaccc acgacgacga catggccatg ggcgccgtcc aggccatccg
     3001 caacgccaac cgccaggacg agatgttcat caccggcgcc ggcgggtcca aggacgccat
     3061 gaacctcatc gccgagggcg acctgtaccg ggccaccttc ctctacaacc cctccatgtc
     3121 cgcctccgcc atctcgctcg ccaagctgat cgccgagcgc aagggtctca gcgagctggt
     3181 cgagcccgag gtcccgagcc gcgtcgagct gcccgcgacc accgtgacca aggacaacgt
     3241 ggcctcggtg cagaacctcg g
//
LOCUS       DASSWR010000030         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1074870, whole genome shotgun sequence.
ACCESSION   DASSWR010000030 DASSWR010000000
VERSION     DASSWR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074870"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFO47_00570"
     CDS             <1..600
                     /locus_tag="VFO47_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET8787222.1"
                     /translation="EEVQKRRSLFADRLGQRVGAAGLRLVDDGRLVAGPGAAPFDGEG
                     VPTRRTVLIDDGVLACFLHNTATAARDGGGAGSTGNARRGSFKSTPGVSAHNLFLEPG
                     ELDQAGLLARAGEGLLVQDVSGVHSGANPITGDFSVGVSGLLIRDGQLAEPVREATVA
                     AHLLDILKGIDAVGSDLRFTTGSIGGSSLLIGEMTVAGA"
     gene            764..1495
                     /locus_tag="VFO47_00575"
     CDS             764..1495
                     /locus_tag="VFO47_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787223.1"
                     /translation="MADRPLAVLVHGVTSSSRTWWRVGPALAGRGFRVVAVDLRGHGA
                     SPRTVAGLALADLAADVAETVEGPVELLVGHSLGALVALDLVGRRPGFARRLVLEDPP
                     GSSSIDWAGLAAGIEADTRRAVADPDGLRRELEAANPAWPGGEAERRVADLADCDGQA
                     IAAALRRGVPFDLPALLAAARLPVLMLLADEALGSNLTGLDRKAAVEALGGGATRVLP
                     AGHSVHREALDPWLAAVDTWLAAQP"
     gene            complement(1492..2262)
                     /locus_tag="VFO47_00580"
     CDS             complement(1492..2262)
                     /locus_tag="VFO47_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787224.1"
                     /translation="MVNMVTSSDGTSIAFERMGAGPPLVMVDAAGSYRDFRPLRPPVE
                     LLSRHFTVYLYDRRGRGASGDTQPYAVEREVDDLAALIAEAGGSASVYAMSSGGLLAL
                     HAAASGLAIPKLALFEPPIEANELPTGETEFTAELAGLVAAGRRRAAVELFHRGIGVP
                     DEVMAQMTPAVWAALEAVAPTLVYDCRLSDAMSLPLVRTVTAPTLVLDSQGSTGDLTG
                     MSAAVVKALPNGSHRSLAGAWHGVPDETLAPVLIEFFG"
     gene            2327..2695
                     /locus_tag="VFO47_00585"
     CDS             2327..2695
                     /locus_tag="VFO47_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8787225.1"
                     /translation="MRYLCLVYADEARLAELSQGELDTLIDETLAANEELAASGHLVL
                     AQALEQVDAAATVRVRDGRLSATDGPFAETREQLGGFVLIEARDLNEAIRIAGRLPSA
                     RLGSIEVRPVIDLLRERSPR"
     gene            2692..3984
                     /locus_tag="VFO47_00590"
     CDS             2692..3984
                     /locus_tag="VFO47_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8787226.1"
                     /translation="MSSGGADDPRARIDRLYRTESRRVLATLIRLLGDFDLAEEALQD
                     AFMAAAERWPRDGVPANPRAWLISAGRFKAIDRLRRRARFDAVLGELAARLDDRVAAD
                     PADQDDQTLEDDRLRLVFTCCHPALPPDAQVAMTLREVCGLTTEEIARAFLTGPPTVA
                     QRIVRAKAKIRAAGIPYEVPPPEELPERLDTVLAVVYLVFTEGYAPASGDAVVRADLA
                     DEAIRLGRLLAELLPEPEVLGLLALMLLQDARRAARTAGGTDLVLLGDQDRSRWDQAR
                     VAEGAALLGRAASSGRVGPYALQAAIAATHAGAATAEATDWDRIVGLYDRLASVDPSP
                     VVELNRAAAISVRDGPASGLALVDALLDGGQLGSYPLAHATRADLLRRLGRTADARAA
                     YERALALTRHEPERRFLRQRLEELAAGPAGDPAPERRR"
BASE COUNT          452 a         1592 c         1506 g          488 t
ORIGIN      
        1 gaggaggtgc agaagcgccg cagcctgttc gccgaccgcc tcggccagcg ggtcggcgcg
       61 gccgggctgc ggctggtcga cgacggccgc ctggtcgccg ggccgggcgc ggccccgttc
      121 gacggcgagg gcgtccccac ccggcgcacg gtgctgatcg acgacggcgt gctggcctgc
      181 ttcctgcaca acaccgccac cgcggcccgc gacggcggcg gggccggctc caccggcaac
      241 gcccgccggg gctcgttcaa gagcaccccg ggggtctcgg cccacaacct gttcctggag
      301 ccgggcgagc tcgaccaggc cgggctgctg gcccgggccg gcgagggcct gctcgtccag
      361 gacgtgtccg gggtccactc gggcgccaac ccgatcaccg gcgacttctc cgtcggggtc
      421 agcggcctcc tgatccgcga cggccagctg gccgagccgg tccgggaggc gaccgtcgcc
      481 gcccacctcc tcgacatcct caagggcatc gacgccgtcg gctccgacct ccgcttcacc
      541 accggctcca tcggcggctc cagcctcctc atcggcgaga tgaccgtcgc cggcgcctga
      601 ggtgccccgc ctcgccaccc gttcgtgggt cgcgtcgtgg gtcgcgccgg gttcgtcggg
      661 cgcgccggag cccgcccctc cccggttccc ggggcccccg aaccgggagg gtagcccagc
      721 ccggcccgag ggccgggggg ctgtccacag gccctgcgcc gaggtggccg accggcccct
      781 ggccgtgctg gtccacgggg tgacgtcgtc gtcgaggacc tggtggcggg tgggtccggc
      841 cctggccggg cgggggttcc gggtggtggc cgtcgacctg cgggggcacg gggccagccc
      901 gcggacggtg gccgggctgg ccctcgccga cctggccgcc gacgtcgccg agacggtcga
      961 ggggccggtg gagctgctgg tcggccactc gctgggggcg ctggtggcgc tcgacctggt
     1021 cggccggcga cccgggttcg cgcgccggct ggtgctggag gacccgcccg ggtcgagctc
     1081 gatcgactgg gccggcctgg ccgccgggat cgaggccgac acccgacggg ccgtcgccga
     1141 cccggacggg ctccgccgcg agctggaggc cgccaacccc gcctggcccg gcggcgaggc
     1201 cgagcgccgg gtcgccgacc tggccgactg cgacggccag gcgatcgccg ccgccctccg
     1261 ccggggcgtc cccttcgacc tgcccgccct gctcgccgcc gcccggctcc ccgtcctcat
     1321 gctcctggcc gacgaggccc tcggctccaa cctgaccggc ctcgaccgca aggccgccgt
     1381 cgaagccctc ggcggcggcg ccacccgcgt cctccccgcc ggccacagcg tccaccgcga
     1441 ggccctcgac ccctggctgg ccgccgtcga cacctggctg gcggcccagc cctagccgaa
     1501 gaactcgatc aggaccggcg cgagcgtctc gtcgggcacg ccgtgccagg cgccggccag
     1561 gctgcggtgg ctgccgttgg gcagcgcctt caccaccgcc gccgacatgc cggtgaggtc
     1621 gccggtgctg ccctggctgt cgaggaccag cgtgggcgct gtcacggtgc ggacgagcgg
     1681 gagcgacatg gcgtccgaga gccggcagtc gtagacgagc gtgggcgcga ccgcctccag
     1741 ggccgcccag accgccggcg tcatctgggc catgacctca tcggggacgc cgatcccgcg
     1801 gtggaagagc tcgaccgcgg cccggcgccg gccggccgcc accagcccgg ccagctcggc
     1861 cgtgaactcc gtctcgcccg tggggagctc gttggcctcg atcgggggct cgaacagcgc
     1921 cagcttcggg atggcgaggc cgctcgcggc ggcgtgcagg gcgagcagcc cgccggagga
     1981 catggcgtac accgaggccg acccgccggc ctcggcgatc agcgccgcca ggtcgtcgac
     2041 ctcgcgctcg accgcgtacg gctgggtgtc gccgctggcg ccccggccgc ggcggtcgta
     2101 gaggtagacg gtgaagtgcc tggacagcag ctcgaccggc ggccgcaggg ggcggaagtc
     2161 ccggtagctg ccggcggcgt cgaccatgac gagcggcggg ccggcgccca tgcgctcgaa
     2221 cgcgatcgag gtcccgtcgc tggaagtgac catgttcacc atgtcggtgc tcctttctcc
     2281 tgctaggttg tcgaacgccg ccggccggga tcgacaggag accgcgatga gatacctctg
     2341 cctggtctat gccgacgagg ccaggctcgc cgagctgtcg cagggcgagc tcgacacgct
     2401 gatcgacgag acgctcgccg ccaacgagga gctggctgcc agcgggcacc tggtcctcgc
     2461 ccaggcgctg gagcaggtgg acgcggccgc caccgtgcgg gtgcgcgacg gcaggctctc
     2521 ggcgaccgac ggcccctttg ccgagacgcg cgaacagctc ggcgggttcg tgctcatcga
     2581 ggcccgggac ctcaacgagg cgatccggat cgccggccgg ctccccagcg cgcgtctcgg
     2641 cagcatcgag gtgcggcccg tcatcgacct gctgcgggag cgctcgcccc ggtgagctcc
     2701 ggcggcgccg acgacccgcg ggcgcggatc gaccgcctct accggaccga gtcgcgccgg
     2761 gtgctggcca ccctgatccg cctgctgggc gacttcgacc tggccgagga ggcgttgcag
     2821 gacgccttca tggcggcggc ggagcgctgg ccccgggacg gcgtccccgc gaacccgcgt
     2881 gcctggctca tctcggccgg gcgcttcaag gcgatcgacc ggctgcggcg ccgggccagg
     2941 ttcgacgcgg tgctcggcga gctggccgcc cgcctcgacg accgggtggc cgccgacccc
     3001 gccgaccagg acgaccagac cctggaggac gaccggctgc ggctcgtctt cacctgctgc
     3061 catcccgcgc tgccgcccga cgcccaggtg gcgatgaccc tgcgcgaggt gtgcggcctc
     3121 accaccgagg agatcgcccg cgccttcctg accggtccac ccaccgtggc ccagcgcatc
     3181 gtgcgcgcca aggcgaagat ccgggccgcc ggcatcccct acgaggtgcc gccgcccgag
     3241 gagctgccgg agcgcctcga caccgtcctg gctgtcgtgt acctggtctt caccgagggc
     3301 tacgcgccgg cctccgggga cgcggtggtc cgcgccgacc tggcggacga ggcgatccgc
     3361 ctcggccggc tgctggccga gctcctcccg gagccggagg tgctcgggct gctcgccctg
     3421 atgctgctcc aggacgcgcg ccgcgccgcc cggaccgccg gcggcaccga cctggtcctg
     3481 ctgggcgacc aggaccgctc gcgctgggac caggcccgcg tcgccgaggg agcggcgctg
     3541 ctcgggcggg cggcgtcgtc ggggcgggtc ggtccctacg ccctccaggc ggcgatcgcc
     3601 gccacccacg ccggggcggc caccgccgag gccaccgact gggaccggat cgtcgggctg
     3661 tacgaccgcc tggcctccgt ggacccctcg ccggtggtcg agctgaaccg ggccgccgcc
     3721 atctcggtgc gcgacggccc ggcgtccggc ctggccctgg tcgacgccct cctcgacggc
     3781 ggccagctcg gctcctaccc cctggcccac gccacccggg ccgacctgct ccgacggctg
     3841 ggacggaccg ccgacgcccg cgccgcctac gagcgcgctc tcgccctgac ccgccacgag
     3901 cccgagcggc ggttcctccg gcaacggctg gaggagctgg cggccggccc cgccggcgac
     3961 ccggcgcccg aacggcgacg gtgacccggc gcggcgtggc cctcagccac cgttccccgg
     4021 gatcgccccg gcggcgcg
//
LOCUS       DASSWR010000031         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1216238, whole genome shotgun sequence.
ACCESSION   DASSWR010000031 DASSWR010000000
VERSION     DASSWR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216238"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFO47_00595"
     CDS             complement(<1..986)
                     /locus_tag="VFO47_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8787227.1"
                     /translation="MRHRGRPGRAGRGHALAEGGLEPLVLEARRRVGGRVHSRRLANG
                     AVVELGAEFVEADHETLVATAARLGLAMAPAGMAYGDRDPRGGLGTDRATVLAEVARL
                     RDLLTAPPAGGPGEPGPGGPDLLGPGRSVAEVLDGLPLDRAAREAIAARLQVSTGQPV
                     GELAAAVLGHAGSSFSTRESLRVAGGNQRIALRLAERLPGAVHLGVPVQAVSWSGPGV
                     RVAVEGAELDAEACLLAVPASVIGRIRFDPPLPDWKAGALGRVAYGHAAKLFVPLRRV
                     PPPSAVLSVPDHYWTWTAKGADGAVQPVVSAFAGSAPALAALEVSAGPATWRR"
     gene            1033..2112
                     /locus_tag="VFO47_00600"
     CDS             1033..2112
                     /locus_tag="VFO47_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET8787228.1"
                     /translation="MPDEEARIALFIDHENLVIGAREIGRGFDVGPIMDALAERGRVV
                     ARRAYADWTLFAGDRRTLVEHNCELIDIPQRTGAVRKNAADIKLAVDAMELAYERGFV
                     STFVIASGDSDFTPLVAALRALNRRVIGIGVKGSTSSLLPPACDEFLFYDRLPGVGEL
                     VPQGSRRAGNGGGNGGARPPRRASKPRKDDGAKPAGQRDLQELQRQLTQTLAGLKQSG
                     AEPVLASSLKRALLRKDPTFAESDYGFRAFRELLGQAERNGIVALTEGQAPGDPAVDF
                     PESGQADKAFDLLRQTLLELQGEDDEVPLSGLKDQMRKRDPNWSEKDFGYAGFLQFVK
                     AAAAKGTVNMEWDDAEGDYFLYVPD"
     gene            2715..>2910
                     /locus_tag="VFO47_00605"
     CDS             2715..>2910
                     /locus_tag="VFO47_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8787229.1"
                     /translation="MDQTLMLPATDVAAMLRRQEVSSRELTEALLARIEAVNPALNAV
                     VELRAEAALREAATADEAAAR"
BASE COUNT          381 a         1095 c         1122 g          312 t
ORIGIN      
        1 cggcgccagg tcgcggggcc ggcgctgacc tccagggcgg ccagggcggg ggcggagccg
       61 gcgaaggcgc tcaccaccgg ctggacggcg ccgtcggcgc ccttggcggt ccaggtccag
      121 tagtggtcgg ggacggacag gacggcgctg ggcggcggca cccggcgcag gggcacgaac
      181 agcttggccg cgtggccgta ggcgacccgg ccgagggcgc cggccttcca gtccgggagg
      241 ggcggatcga agcggatccg gccgatgacc gaggcgggca cggccagcag gcaggcctcg
      301 gcgtccagct cggcgccctc gaccgcgacc cgcacgcccg ggcccgacca gctgaccgcc
      361 tgcacgggca cgcccaggtg gacggcgccg gggagccgtt cggccagccg cagggcgatc
      421 cgctggttgc cgccggcgac gcggaggctc tcgcgggtcg agaagctcga gccggcgtgg
      481 ccgaggacgg ccgcggccag ctccccgacc ggctggccgg tcgacacctg caggcgggcg
      541 gcgatggcct cgcgggcggc ccggtcgagg ggcagcccgt ccagcacctc ggccaccgac
      601 cgcccgggcc ccaggaggtc ggggccgccc gggccgggct cgccggggcc gccggcgggc
      661 ggcgcggtca gcaggtcccg gaggcgggcg acctcggcca ggacggtggc ccggtcggtg
      721 cccaggccgc cgcgagggtc gcggtcgccg taggccatgc cggccggggc catggccagg
      781 cccaggcggg cggcggtggc gaccagcgtc tcgtggtcgg cctcgacgaa ctcggccccg
      841 agctcgacca cggcgccgtt ggccagccgc cgcgagtgga cccgcccccc gacccgccgg
      901 cgggcctcca gcaccagcgg ctccagcccg ccctcggcca gggcatggcc gcggccagcc
      961 cggccaggcc ggccccgatg acgcacaccc gggtcgtagg cacggccaca ggttacggcg
     1021 tagtgttcac ccatgcctga cgaggaagca cgcatcgcgc tgttcatcga ccacgagaac
     1081 ctggtcatcg gggccaggga gatcggccgg ggcttcgacg tcggcccgat catggacgcc
     1141 ctggccgagc ggggccgggt ggtggcccgg cgggcctatg ccgactggac gctgttcgcc
     1201 ggcgaccgcc gcaccctggt cgagcacaac tgcgagctga tcgacatccc ccagcggacc
     1261 ggggcggtgc gcaagaacgc cgccgacatc aagctggccg tggacgccat ggagctggcc
     1321 tacgagcggg ggttcgtgtc caccttcgtg atcgcctcgg gggacagcga cttcaccccg
     1381 ctggtggcgg ccctgcgggc cctgaaccgg cgggtcatcg gcatcggggt caagggctcg
     1441 acctcgagcc tgctcccccc ggcctgcgac gagttcctgt tctacgaccg cctgcccggg
     1501 gtcggcgagc tggtccccca gggaagccgg cgggccggca acggcggcgg caacgggggc
     1561 gcccggccgc cgcggcgggc cagcaagccc cgcaaggacg acggcgccaa gccggccggc
     1621 cagcgggacc tgcaggagct gcagcgccag ctcacccaga ccctggccgg gctgaagcag
     1681 agcggcgccg agcccgtgct ggcctcgtcg ctcaagcggg ccctgctgcg caaggacccg
     1741 accttcgccg agtccgacta cggcttccgc gccttccggg agctgctcgg ccaggccgag
     1801 cgcaacggca tcgtcgccct caccgagggc caggcccccg gcgacccggc ggtcgacttc
     1861 cccgagtccg gccaggccga caaggccttc gacctgctcc gccagaccct gctcgagctc
     1921 cagggcgagg acgacgaggt ccccctgtcc ggcctcaagg accagatgcg caagcgcgac
     1981 cccaactggt ccgagaagga cttcggctac gccggcttcc tccagttcgt caaggccgcc
     2041 gcggccaagg gcaccgtcaa catggaatgg gacgacgccg agggcgacta cttcctgtac
     2101 gtccccgact gagccggtgc cgctggaggt ccgggaggcg cggaccgacg ccgaggtgga
     2161 ggcggccggg cgggtgacgg tggcggccaa cgccgagttc gcccccgccg accctgacgg
     2221 gccgttcgcg ccctcgtggc ggcgctacct ggccgagatg gccgacgcgg ccgcccgggc
     2281 cgcccagggg gtgctgctgg tcgcggtcga ggacgggcgg gtggtgggga cggtcacgct
     2341 ctacctggct cccgggtcga tgcagtggcg gcccgacgac gccatgttcc ggctcctggc
     2401 cgtcgacccg gcggcccgcg ggcgcggcat cggccgggcc ctgctccagg cctgcctgga
     2461 ccgggcccgg gcggccggca agcgccgcat ggccctgcac accaccgagt ggatgcccgt
     2521 ggcccgggcc atgtacgagc gggccggctt caagcgcgaa cccgagggcg acgaggagct
     2581 tcccggcgtc accatcatcg cctacgcggc cgagctggaa cccgccgcgg acccagaggc
     2641 ttgtccctgc ccctggggga ggccctacac cggtgggtgc cgggcgccac cttccgatga
     2701 cgggcggaca cgacatggac cagaccctga tgctgccggc gaccgacgtg gccgccatgc
     2761 tgcgccgcca ggaggtgtcg tcccgggagc tgaccgaggc cctgctggcg cggatcgagg
     2821 ccgtcaaccc ggcgctcaac gccgtggtcg agctgcgggc cgaggcggcg ctgcgtgagg
     2881 ccgccaccgc cgacgaggcg gccgcccggg
//
LOCUS       DASSWR010000032         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_114138,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000032 DASSWR010000000
VERSION     DASSWR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114138"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFO47_00610"
     CDS             <1..491
                     /locus_tag="VFO47_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET8787230.1"
                     /translation="GTAGSGAVGADGRVAVHVAGRVRRPGLVQLPAGSRVQDAIRAAG
                     GAISGADLDAVNLARKLTDGEQIRVPAPGDPAPPPQGVAAPGASGTPGGPGAVLSAPL
                     DLNTATVEQLDTLPGVGEVTAGRIVAYRTAHPFTAVDELLEVPGIGQRRYDQLKDLVT
                     VA"
     gene            488..>2157
                     /locus_tag="VFO47_00615"
     CDS             488..>2157
                     /locus_tag="VFO47_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET8787231.1"
                     /translation="MTPAPPPRPVRHPDADPDVDGVALAAATATGVFLGTRFAAAAAW
                     MVVAASLAVACLGAGSSSRLRDEGTAAVVGVLVLATLLAAGAAAASVRATAVRDGILA
                     RRVQHQGRVEGQGRVEDPGWAGPRGRVEVAASVAEEPQRLRFGGHWVVLAVHQVRQAG
                     RTYRTRERAGMILPRGRLPPPGAPRGPAVGGPGAAQPTRPPATAGPLVDRGEGRLAVG
                     DRLRVRASVGRARWSDALGRRPPVVLRDPVIEERAPPAGAALRVSELVRDAARERALA
                     SLAPERAGLLVGMALGDTSLLPVELEGEFRAAGLTHLMAVSGANLAVVLAAGLSLAGL
                     AGAGRRGLAVAGIVLVVVLVVVTRWEPSVLRAGVMAGLVLLGVATGRGPGGRRALCLA
                     VVVLLLADPGLAGALGFRLSVAATAGVLWFGPLVARALPDRVPERVGKAVGMTLGAQA
                     TAVPALALALGPVSLAGLPANLLGLPLAGGPMLLGVIAAATAPVAPGVAALACRLADP
                     FLLALIAVARWAADLPGGTVTLAGPARVAPVVVVVVLGLLTAARRRRSP"
BASE COUNT          164 a          869 c          840 g          284 t
ORIGIN      
        1 ctgggactgc cggctcgggg gccgtcgggg ccgacggtcg ggtcgccgtt cacgtggccg
       61 gccgggtccg ccgcccgggg ctggtccagc tgcccgccgg cagccgggtc caggacgcca
      121 tccgggccgc cggcggcgcc atctccggcg cggacctgga cgcggtcaac ctcgcccgca
      181 agctgaccga cggcgagcag atccgggttc ccgcccccgg cgaccccgcc ccgccgcccc
      241 agggcgtggc cgctcctggc gcttccggca ctccgggtgg tccgggcgcg gtcctgtccg
      301 cgccgctcga cctcaacacc gccaccgtcg agcagctcga caccctcccc ggcgtcggcg
      361 aggtcaccgc cggccgcatc gtcgcgtacc ggaccgccca tcccttcacc gccgtcgacg
      421 agctcctcga ggtccccggc atcggccagc gccgctacga ccagctcaag gacctggtga
      481 cggtcgcgtg acgccagccc caccgccccg tcccgtccgc caccccgacg cggaccccga
      541 cgtcgacggg gtggcgctgg cggcggccac cgccaccggg gtcttcctcg gcacccggtt
      601 cgccgcggcc gcggcctgga tggtcgtggc cgcctccctg gccgtggcct gcctgggggc
      661 gggcagctcg tcccgcctcc gggacgaagg caccgccgcc gtggtcggcg tgctcgtcct
      721 cgccaccctg ctggcggccg gggccgccgc cgcctcggtc cgggcgacgg cggtccggga
      781 cggcatcctg gcccgccggg tccagcacca gggccgggtc gagggccaag gtcgggtcga
      841 ggaccccggc tgggccgggc cccggggccg ggtcgaggtg gccgccagcg tcgccgagga
      901 gccgcagcgg ctccgcttcg gcggccactg ggtcgttctt gctgtgcacc aggtccgcca
      961 ggccggccgc acctaccgca cccgcgaacg cgccggcatg atcctccccc ggggccgcct
     1021 ccctcctccg ggcgcacccc gcggtccggc cgtcggtggt ccgggtgccg cccagcccac
     1081 ccggcccccg gccaccgcag gacccttggt cgaccgtggg gagggtcggc tcgcggtagg
     1141 ggaccggctg cgggtccggg cgtcggtagg ccgggcccgg tggagcgatg cgctggggag
     1201 gcggccgccg gtcgtgctcc gggaccccgt gatcgaggag cgggcgccgc cggccggtgc
     1261 cgccctgcgg gtcagcgagc tggtgcggga cgcggcgcgc gagcgggcgc tggccagcct
     1321 ggcgccggaa cgtgccgggc tgctcgtcgg catggcgctc ggcgacacgt ccctgctccc
     1381 ggtggagctg gaaggcgagt tccgcgcggc cgggctgacg cacctgatgg cggtcagcgg
     1441 cgccaacctg gccgtggtcc tcgccgccgg gctgtcgctg gccgggctcg ccggcgccgg
     1501 tcgccgcggc ctggccgtcg ccgggatcgt cctggtggtg gtgctggtgg tcgtcacccg
     1561 ctgggaaccg agcgtcctcc gggccggcgt gatggccggt ctggtgctgc tcggggtcgc
     1621 caccgggcgg ggcccgggtg gccgccgggc cctctgcctg gccgtggtcg tgctgctgct
     1681 ggccgacccg gggctcgccg gtgccctcgg gttccggctg tcggtggcgg ccaccgccgg
     1741 cgtcctctgg ttcggcccgc tcgtggccag ggcgctcccg gaccgggttc ccgagcgcgt
     1801 cgggaaggcg gtcggcatga ccctcggcgc ccaggccacg gccgtgcccg cgctcgccct
     1861 ggccctcggc ccggtctccc tggccggact gccggccaac ctgctcggcc ttcccctggc
     1921 cggcggtccg atgctgctcg gggtgatcgc cgcggccacg gccccggtcg cgcccggggt
     1981 cgcggccctg gcctgccggc tggccgaccc gttcctgctc gccctgatcg cggtcgcccg
     2041 ctgggccgcg gacctgcccg gcgggacggt caccctggcc ggcccggccc gggtcgcgcc
     2101 ggtcgtggtc gtggtggtcc ttgggttgct gacggcggcg cggcgtcggc gttcgcc
//
LOCUS       DASSWR010000033         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1272741, whole genome shotgun sequence.
ACCESSION   DASSWR010000033 DASSWR010000000
VERSION     DASSWR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272741"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFO47_00620"
     CDS             complement(<1..332)
                     /locus_tag="VFO47_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787232.1"
                     /translation="MTARLTLPRTAAWVWHGGPRSIFGLEAVFVRCASGTSGSAGSGF
                     SYADNFHAWLQARGPAVVPWSWLGPPHSADGVASADALSDIAPGQHVYVVEVGPGTSP
                     DQVTAFAAR"
     gene            complement(422..751)
                     /locus_tag="VFO47_00625"
     CDS             complement(422..751)
                     /locus_tag="VFO47_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787233.1"
                     /translation="MVTARKQVGTSSTYGALRNWQPPRPSYDQLRQAGQQATAGDLEV
                     LLRTTEVARRLQVSARTVLAWAQAGKLPSILTPGGHRRYPAQGVEQVLQTMTAAGEPR
                     TTQGPSR"
     gene            982..3477
                     /gene="leuS"
                     /locus_tag="VFO47_00630"
     CDS             982..3477
                     /gene="leuS"
                     /locus_tag="VFO47_00630"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET8787234.1"
                     /translation="MPLQHSEEISVDAERYDPQQVEPKWVAAWEELALYRTDESETSR
                     PRFYALDMFPYPSGDLHMGHLEAFSGGDVIARLKWMQGYNVLHPIGWDAFGLPAENAA
                     IKRGVHPKPWTYQNIDAQRATFHRLGISFDWSRALVTCDPDYYRWTQWLFLRFFERGL
                     AYRKASPVNWCPNDQTVLANEQVINGHCERCDALVEKRELTQWFFKITDYAQRLLDDM
                     EGLNWSDKVLTQQRNWIGRSEGADVDFQVAETGETVRVFTTRPDTLFGATFFVLAPEH
                     PLAERLVAGTEREAEFRTFRERVGRETEIERLSTERDKQGMFLGAHAVNPVNGELIPI
                     WAADYVLMEYGTGAIMAVPAHDQRDLEFARKYRLPVRVVVQPPGEPPLDGSTLTEAMS
                     HEGVLVNSGPYDGLPATEEGIPRITADLEAQGKGGPSVNFRLRDWLLSRQRYWGCPIP
                     IVHCPACGEVPVPDDQLPVLLPEDVTDFRPKGLSPLATSEAWVNVDCPRCGGPARRET
                     DTMDTFVDSSWYFLRYTGLEPDRPFDPERVAGWMPVHQYTGGIEHAILHLLYARFFTK
                     VLCDMGLVGFTEPFTSLLNQGMVVMQGAAMSKSKGNLVEPSEIIDEHGADVARLTILF
                     AGPFEDDVDWADVSPEGMARWIQRVWRAVHAAVADPPAGEGWPEDPELVRLAHRTTAG
                     VTDDLERFRFNTAISKLMVLSNGLADAIRAGRGSAAQRREVAERLVLLLAPLAPFVTE
                     ELWRVLGGSSSVHRQAWPGFDADLVRSERVTCVVQVDGKVRDRIEVAPDAPEDELREL
                     AMASERVRAALAGRRVARVVVVPPKLVNLVTAG"
BASE COUNT          506 a         1301 c         1273 g          517 t
ORIGIN      
        1 cgggccgcga acgccgtcac ctggtcgggc gaggtcccgg gcccgacctc gacgacgtag
       61 acgtgctggc cgggggcgat gtcggagagg gcgtcggcgc tggccacccc gtcggcgctg
      121 tggggcggcc ccaaccacga ccaggggacc accgccggcc cgcgtgcctg cagccaggcg
      181 tggaagttgt cggcgtagct gaacccggac ccggcgctcc cgctggtgcc gctggcgcag
      241 cgcacgaaca ccgcctcgag gccgaagatc gacctgggcc cgccgtgcca gacccaggcc
      301 gccgtcctgg gaagggtcaa ccgggccgtc atccatcacc tccaagccct cggtcgcgat
      361 gcagcagtat cgcaaaacag cggcgggccg acccgagggc cggcccgccg ctggtcgttc
      421 gtcagcggct gggcccttga gtggtgcgag gctcgccggc ggcggtcatg gtctgaagca
      481 cctgctcgac gccctgcgcc ggatagcgac ggtgaccacc cggggtcagg atgctcggca
      541 gcttccccgc ctgagcccag gcgaggaccg tacgggcgct cacctggagc ctgcgggcaa
      601 cctcggtggt gcggagcagg acctccaggt cgccggcggt cgcctgctgt ccagcctggc
      661 ggagctggtc ataggacggc cgcggcggct gccagttgcg gagtgcgcca taggtgctcg
      721 aggtaccgac ctgcttgcga gcggttacca ctctgcctcc cctctggtgg catgtcggcg
      781 gaggacgtgc ccgctgccac cgagcgcgct acgtccggct cgttccactt cgacagtatg
      841 ggcgggactt ttgcactcga cgggtccggc tgtccagtgg acaaaatgcc cggctgccct
      901 aacgagtccc caacgcggct ggaatagcgc tggtggcctg ctgttatgct tgtcggaggt
      961 ggccggaagg ccccgttccg catgccccta cagcattctg aggagatttc cgtggacgcc
     1021 gagcgctacg acccccagca ggtggagccc aagtgggtgg cggcctggga ggagctggcg
     1081 ctctaccgta ccgacgagag cgagaccagc cgtccgcgct tctacgccct ggacatgttc
     1141 ccgtacccct cgggcgacct gcacatgggc cacctggagg cgttctcggg cggtgacgtc
     1201 atcgcccggc tgaagtggat gcagggctac aacgtgctgc acccgatcgg ctgggacgcc
     1261 ttcggcctgc ccgccgagaa cgcggccatc aagcgcgggg tccatcccaa gccctggacc
     1321 taccagaaca tcgacgccca gcgggccacc ttccaccggc tggggatctc cttcgactgg
     1381 tcgcgggccc tggtcacctg cgacccggac tactaccgct ggacccagtg gctgttcctg
     1441 cgcttcttcg agcgcggcct ggcctaccgc aaggcctcgc cggtcaactg gtgccccaac
     1501 gaccagacgg tgctggccaa cgagcaggtc atcaacggcc actgcgagcg ctgcgacgcc
     1561 ctggtcgaga agcgcgagct gacccagtgg ttcttcaaga tcaccgacta cgcccagcgg
     1621 ctgctggacg acatggaggg cctgaactgg agcgacaagg tcctgaccca gcagcgcaac
     1681 tggatcgggc gcagcgaggg ggccgacgtc gacttccagg tggccgagac cggcgagacg
     1741 gtgcgggtgt tcaccacccg cccggacacg ctgttcgggg ccacgttctt cgtgctcgcc
     1801 cccgagcacc cgctggccga gcgcctggtc gccggcaccg agcgcgaggc cgagttccgc
     1861 accttccggg agcgggtggg ccgcgagacc gagatcgagc ggctgtcgac cgagcgcgac
     1921 aagcagggca tgttcctggg ggcgcacgcc gtcaacccgg tcaatggtga gctgatcccc
     1981 atctgggccg ccgactacgt gctgatggag tacggcaccg gcgccatcat ggccgtgccc
     2041 gcccacgacc agcgcgatct ggagttcgcc cgcaagtaca ggctgccggt gcgggtggtg
     2101 gtgcagccgc cgggggagcc gccgctggac ggctccaccc tgaccgaggc catgagccac
     2161 gagggcgtgc tggtcaactc cggcccgtat gacggcctgc ccgccaccga ggagggcatc
     2221 ccccggatca ccgccgacct ggaggcccag ggcaagggcg gcccgtcggt caacttccgc
     2281 ctgcgcgact ggctgctctc gcgtcagcgc tactggggct gcccgatccc gatcgtgcac
     2341 tgcccggcct gcggcgaggt gccggtcccc gacgaccagc tgccggtgct gctgcccgag
     2401 gacgtcaccg acttccggcc caagggcctg tcgcccctgg ccacctccga ggcctgggtg
     2461 aacgtcgact gcccgcgctg cggcggcccg gcccggcgcg agaccgacac catggacacc
     2521 ttcgtcgact cctcctggta cttcctgcgc tacaccgggc tggagccgga ccggccgttc
     2581 gaccccgagc gggtggccgg gtggatgccg gtgcaccagt acaccggcgg gatcgagcac
     2641 gccatcctgc acctgctgta cgcccggttc ttcaccaagg tcctgtgcga catgggcctg
     2701 gtcggcttca ccgagccgtt cacctcgctg ctcaaccagg gcatggtggt gatgcagggg
     2761 gcggccatgt ccaagtccaa gggcaacctg gtcgagccgt cggagatcat cgacgagcac
     2821 ggcgccgacg tggcccgcct gaccatcctg ttcgccgggc cgttcgagga cgacgtcgac
     2881 tgggccgacg tgtcgccgga gggcatggcc cgctggatcc agcgggtctg gcgggccgtc
     2941 cacgcggccg tggccgaccc gccggcgggg gagggctggc ccgaggaccc cgagctggtc
     3001 cgcctggccc accggaccac cgccggggtc accgacgacc tggagcggtt ccgcttcaac
     3061 accgccatct ccaagctgat ggtgctgtcc aacggcctgg ccgacgccat ccgggccggc
     3121 cggggcagcg ccgcccagcg gcgcgaggtc gccgagcggc tggtcctgct gctggccccg
     3181 ctggccccgt tcgtgaccga ggagctgtgg cgggtccttg gcggctcgag ctcggtccac
     3241 cggcaggcct ggcccggctt cgacgccgac ctggtccgca gcgagcgggt cacctgcgtg
     3301 gtccaggtcg acggcaaggt ccgcgaccgc atcgaggtcg cccccgacgc ccccgaggac
     3361 gagctgcggg agctggccat ggcctccgag cgggtccgcg ccgccctggc cggccgtcgg
     3421 gtggcccggg tcgtggtcgt cccgcccaag ctggtgaacc tggtcaccgc cggctgaccg
     3481 gggacgccgg gcggcacggg acgagcgcct cggaccggaa ctcgttcagg gccgccaccg
     3541 tctccggcag cggggtcgtc accgccagcc gtggggcggc gtcggccttc tccccgg
//
LOCUS       DASSWR010000034         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_368672,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000034 DASSWR010000000
VERSION     DASSWR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368672"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VFO47_00635"
     CDS             <1..1692
                     /locus_tag="VFO47_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8787235.1"
                     /translation="ARAEAMARLRQRARLPLIAVPAGGLVGILGSYLYWTLSPVRVEG
                     VPLTGRLADFGIGGVRTWSLLLALAVLVYGALVLRSRTRTSTQGEALVRLGVGLTVLP
                     PVWALTLVLFLDVEVGRLGPGPWVTAAGGVLAWLGARSLLAAEPAPAATPWARRSMLL
                     DLAVIVVAELVAYGVFLLGIEIDDPGEFLGFFTALTAIAATLSTLGVFKGLAGPFRRN
                     EGVAIALLAVLLLLFPFTQEGNTFWIQVTAQAGLFVLAALGLNIVVGSAGLLDLGYVA
                     FFGIGAYTAALTGNALLTNVNFHIPFLLVVFIVAPVVAAFFGVLLGAPTLRLRGDYLA
                     IVTLGFGEIVRITLNNLDPITRGPNGISSIPDPVIGGWSMGAGFTVAGFEISGNAIYY
                     YLLLALIALTLATIYRLNDSRIGRAWVAVREDEVAAAAMGINTTIIKLLAFGAGAWFA
                     GMSGAVNAHLLTQVSPDSYTFPFSVLILAMVVLGGLGNPPGVVLGAIILTFLPEKLRE
                     FSDIRFLVFGFALILIMRFRPEGLLPSARRRRELHATGETAAAESQQLFDIRSGR"
     gene            1689..>2280
                     /locus_tag="VFO47_00640"
     CDS             1689..>2280
                     /locus_tag="VFO47_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8787236.1"
                     /translation="MTDPTATTAGPAPRPGPAGDGTPILEARKVTRRFGGLVAVREVD
                     LTVGQGQIIGLIGPNGAGKTTFFNCITGMFAPSEGDVLFEGRRINGFSPDRITKLGIA
                     RTFQNLRLFANMTAHENVLVGRHCRTREGVISSVIRGPRFRREERESFERANELLEFV
                     GLAGKGDELARNLPYGDMRRLEIARALATEPKLVLLD"
BASE COUNT          271 a          848 c          789 g          372 t
ORIGIN      
        1 gcccgggccg aggccatggc ccgcctgcgg caacgggccc gcctgcccct gatcgcggtc
       61 cccgccggcg ggctggttgg catcctcggc tcctacctgt actggaccct gtcgccggtg
      121 cgggtcgagg gggtgcccct caccgggcgg ctggccgact tcgggatcgg cggggtgcgg
      181 acctggtcgc tgctgctcgc cctggccgtc ctggtctacg gggcgctggt gctgcgcagc
      241 cggacgcgaa cctcgaccca gggcgaggcc ctggtccgcc tcggcgtcgg gctgaccgtc
      301 ctcccgccgg tctgggcgct caccctggtc ctgttcctcg acgtcgaggt gggcaggctc
      361 ggcccggggc cgtgggtgac ggcggccggc ggcgtgctgg cctggctggg cgcccgcagc
      421 ctgctggcgg ccgagccggc gccggcggcc acgccctggg cgcggcggtc gatgctgctc
      481 gacctggccg tgatcgtggt ggccgagctg gtcgcctacg gggtcttcct gctcggcatc
      541 gagatcgacg acccgggcga gttcctcggc ttcttcaccg ccctgaccgc gatcgcggcc
      601 accctgtcca ccctcggcgt gttcaagggc ctggccggac cgttccgccg caacgagggc
      661 gtcgccatcg ccctgctggc cgtgctcctg ctgctgttcc ccttcaccca ggagggcaac
      721 accttctgga tccaggtgac cgcccaggcc ggcctgttcg tgctggccgc cctcgggttg
      781 aacatcgtgg tcgggtcggc cggcctgctc gacctgggct acgtcgcctt cttcggcatc
      841 ggcgcctaca cggccgcgct caccggcaac gccctgctca ccaacgtgaa cttccacatc
      901 ccgttcctgc tggtggtgtt catcgtggcg cccgtggtgg cggccttctt cggggtcctg
      961 ctgggggcgc cgacgctgcg gctgcgcggc gactacctgg ccatcgtgac cctcgggttc
     1021 ggggagatcg tccgcatcac cctcaacaac ctcgacccga tcacccgcgg ccccaacggc
     1081 atctcctcga tccccgaccc ggtgatcggc ggctggtcga tgggcgccgg cttcaccgtc
     1141 gccgggttcg agatcagcgg caacgcgatc tactactatc tgctgctggc cctgatcgcc
     1201 ctcaccctgg ccaccatcta ccggctcaac gactcgcgca tcgggcgggc ctgggtggcg
     1261 gtgcgcgagg acgaggtcgc ggccgcggcc atgggcatca acaccaccat catcaagctg
     1321 ctggccttcg gggccggggc ctggttcgcc gggatgtccg gggcggtcaa cgcccacctg
     1381 ctcacccagg tgtcgcccga ctcctacacc ttcccgttct cggtgctgat cctggccatg
     1441 gtggtgctgg gcggcctcgg caacccgccc ggggtggtgc tgggggcgat catcctgacc
     1501 ttcctccccg agaagctgcg ggagttctcc gacatccgct tcctggtctt cgggttcgcc
     1561 ctgatcctga tcatgcggtt ccgtcccgag gggctgctgc cctcggcccg ccgccgccgc
     1621 gagctgcacg ccaccggcga gaccgccgcc gccgaatccc aacagctctt cgacatcagg
     1681 agcgggagat gacggatccc acggcgacga cggccgggcc cgcgccgcgg ccgggacccg
     1741 ccggcgacgg cacgccgatc ctggaggcgc gcaaggtgac ccgccggttc ggggggctgg
     1801 tggcggtgcg cgaggtcgac ctcaccgtcg gccagggcca gatcatcggc ctgatcggcc
     1861 ccaacggggc cggcaagacg accttcttca actgcatcac cggcatgttc gcgcccagcg
     1921 agggcgacgt gctgttcgag ggcaggcgga tcaacggctt ctccccggac cggatcacca
     1981 agctcggcat cgccaggacc ttccagaacc tccggctgtt cgccaacatg accgcccacg
     2041 agaacgtcct ggtcgggcgg cactgccgga ccagggaggg cgtcatctcc tcggtgatcc
     2101 gcgggccccg gttccggcgc gaggagcggg agagcttcga gcgggcgaac gagctgctgg
     2161 agttcgtcgg cctggccggc aagggcgacg aactggcccg caacctgccc tacggcgaca
     2221 tgcggcggct ggagatcgcc cgcgccctgg ccaccgagcc caagctggtc ctgctggacg
//
LOCUS       DASSWR010000035         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_57864,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000035 DASSWR010000000
VERSION     DASSWR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57864"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2923)
                     /gene="hrpA"
                     /locus_tag="VFO47_00645"
     CDS             complement(<1..2923)
                     /gene="hrpA"
                     /locus_tag="VFO47_00645"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET8787237.1"
                     /translation="MAGVRRRDARRGVRDRVARLPPGDPIVEGMPALTITYPPELPVS
                     RRRGEIAEAIREHQVVVVAGETGSGKTTQLPKICLELGRGTNGMIGHTQPRRLAARTV
                     AERVASELRTPLGETVGYQVRFTDRVGRDTRVKLMTDGILLAELERDRTLRRYDTIII
                     DEAHERSLNVDFLLGYLARLLPRRPDLKVIITSATIDPERFSRHFGGAPVVEVSGRTY
                     PVEVRYRPLGEEPDDDRDQTQGILDAVDELCREGPGDILVFLSGEREIRDTADALRGH
                     AFPRSPGGVEVLPLYARLAAAEQHRVFRPHAGRRVVLATNVAETSLTVPGIKYVVDPG
                     TARISRYSHRSKVQRLPIEPVSQASANQRKGRCGRTSDGICIRLYPEDDFAGRPEFTE
                     PEILRTNLASVILQMTALRLGDVAAFPFIDPPDRRSVKAGVDLLGELGALEGGRLTTV
                     GRRLAQVPADPRLARMLLEAGRLGCAREVLVIVAALSIQDPRERPAEKQEAADEQHRR
                     FADKGSDFLTWLNLWRYLHERQAELSSNQFRKLCRAEFLNYLRVREWQDLHGQLRSVA
                     RDLGIRLNRPGEEPDADRIHTALLAGLLSHIGLKQGDTREYLGARGTRFAIFPGSALA
                     RKPPQWVMAAELVETSRLWGREAAAIKPEWVEPLAAHLVKRTYAEPHWSRSRGAVMAS
                     ERVTLYGVPIVVDRQVNYGSIDPEVARELFIRHALVEGDWETSHEFFARNRRLLAEVE
                     ELEHRARRRDILVDDHTLFDFYDQRIPDEVVSARHFDAWWKRAREQRPDLLDFERSML
                     ITDAAGAPDAVDYPDTWVQDGHRLRLTYQFEPGSDADGVTVHVPLAVLNQIDPDGFDW
                     QVPGLRQELVTELIRSLPKAIRRGFIPAPDWAAATLERIGPADGPLRDALARELERAG
                     RVEVAPEAFDLSKVPAHQRMTVRVVDRRGRGPARVVGEGKDLETLKRRLAPRMRAALA
                     AA"
BASE COUNT          380 a         1067 c         1085 g          407 t
ORIGIN      
        1 cggcggcggc cagggcggcc cgcatccgcg gggccagccg ccgcttcagc gtttcgagat
       61 ctttgccctc gcccaccacc cgggccgggc cgcgaccccg gcggtccacc acccggacgg
      121 tcatccgctg gtgggcgggg accttggaga ggtcgaacgc ctccggcgcg acctcaaccc
      181 gcccggcccg ctccaactcg cgggccagcg cgtcgcgcag cggcccgtcg gcggggccga
      241 tccgctccag ggtcgcggcc gcccagtcgg gggcggggat gaagccgcgc cggatcgcct
      301 tgggcaggga gcggatcagc tcggtcacca gctcctggcg cagccccggg acctgccagt
      361 cgaagccgtc cgggtcgatc tggttgagga cggccagggg gacgtggacg gtgacgccgt
      421 cggcgtcgct gcccggctcg aactggtagg tgagccgcag gcggtggccg tcctggaccc
      481 aggtgtcggg gtagtcgacg gcgtcgggcg cgccggcggc gtcggtgatc agcatcgagc
      541 gctcgaagtc gagcaggtcg ggccgctgct cgcgggcccg cttccaccag gcgtcgaagt
      601 ggcgggccga gacgacctcg tcggggatgc gctggtcgta gaagtcgaac agggtgtggt
      661 cgtcgaccag gatgtcgcgg cggcgggccc ggtgctccag ctcctcgacc tcggccagga
      721 gccgccggtt gcgggcgaag aactcgtgcg aggtctccca gtcgccctcg accagggcgt
      781 ggcggatgaa cagctcgcgg gccacctcgg ggtcgatcga cccgtagttg acctgccggt
      841 caacgacgat cggcaccccg tagagggtca cccgctcgga ggccatcacc gccccgcgcg
      901 acctcgacca gtgcggctcg gcgtaggtcc gcttgaccag gtgggcggcc agcggctcga
      961 cccactcggg cttgatggcg gccgcctccc gcccccacag ccgcgaggtc tcgaccagct
     1021 cggccgccat cacccactgg ggcggcttcc gggccagcgc cgacccgggg aagatggcga
     1081 accgggtgcc gcgggcaccc aggtactcgc gggtgtcgcc ctgcttgagg ccgatgtggc
     1141 tgagcagccc ggccagcagg gcggtgtgga tgcggtcggc gtcgggctcc tcgccgggcc
     1201 ggttgagccg gatgccgagg tcgcgggcga ccgagcggag ctggccgtgg aggtcctgcc
     1261 actcccgcac ccgcaggtag ttgaggaact cggcccggca cagcttgcgg aactggttcg
     1321 aggacagctc ggcctggcgt tcgtgcaggt agcgccagag gttcagccag gtcaggaagt
     1381 cggagccctt gtcggcgaag cggcggtgct gctcgtcggc cgcctcctgc ttctcggccg
     1441 ggcgctcgcg tgggtcctgg atggacaggg cggccacgat caccagcacc tcgcgggcgc
     1501 agccgagccg cccggcctcc agcagcatcc gggccaggcg ggggtcggcc gggacctggg
     1561 ccagccgccg gcccactgtg gtcagccggc cgccctcgag ggcgcccagc tcaccgagga
     1621 ggtccacccc ggccttgacg ctgcgccggt cgggcgggtc gatgaagggg aaggcggcca
     1681 cgtcgcccag gcgcagcgcc gtcatctgca ggatcaccga ggccagattg gtgcgcagga
     1741 tctccggctc ggtgaactcg ggccggccgg cgaagtcgtc ctctgggtag aggcggatgc
     1801 agatgccgtc ggaggtgcgc ccgcagcggc ccttgcgctg gttggccgag gcctgcgata
     1861 ccggctcgat gggcaggcgc tgcaccttgg accggtggct gtagcgggag atccgggccg
     1921 tgcccgggtc gaccacgtac ttgatgccgg gcacggtcag cgaggtctcg gccacgttgg
     1981 tggccagcac cacccgccgc ccggcgtggg gccggaacac ccggtgctgc tcggccgcgg
     2041 ccagccgggc gtacagcggc agcacctcca ccccacccgg gctcctcggg aaggcatgac
     2101 cccgcagggc gtcggcggtg tcgcggatct cccgctcgcc gctgaggaag accaggatgt
     2161 cgccgggccc ctcccggcac agctcgtcga ccgcgtccag gatcccctgg gtctggtcgc
     2221 ggtcgtcgtc cggctcctcc ccgaggggcc ggtaccggac ctcgaccggg taggtccgcc
     2281 cggacacctc caccaccgga gccccgccga agtgccggct gaaccgctcc gggtcgatcg
     2341 tcgccgaggt gatgatcacc ttgaggtcgg gccgccgcgg cagcagccgg gccaggtacc
     2401 cgagcaggaa atcgacgttg aggctgcgct cgtgggcctc gtcgatgatg atcgtgtcgt
     2461 agcgccgcag cgtccggtcc cgctccagct cggccagcag gatcccgtcg gtcatcagct
     2521 tgacccgcgt atccctgccc acccggtccg tgaaccggac ctggtacccg accgtctccc
     2581 ccagcggcgt ccgcagctcg gacgccaccc gctcggccac cgtccgcgcg gccagccgcc
     2641 gcggctgggt gtgcccgatc atcccgttgg tgccccggcc cagctccaga cagatcttgg
     2701 gcagctgggt cgtcttcccc gacccggtct cccccgccac caccacgacc tgatgctcgc
     2761 ggatggcctc ggcgatctcg ccacgccgcc ggctgaccgg tagttcgggc gggtaggtga
     2821 tggtgagggc aggcatgccc tctacgatag ggtcgccggg cgggagccgg gctacgcgat
     2881 ctcgaactcc ccgtcgcgca tcccggcggc gaacgcccgc cactcgtcgg tgctgaaca
//
LOCUS       DASSWR010000036         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_142643,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000036 DASSWR010000000
VERSION     DASSWR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142643"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFO47_00650"
     CDS             <1..363
                     /locus_tag="VFO47_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8787238.1"
                     /translation="AGAAGYVLKQIRGEELVRAIQAVGAGQNLLDPAVTKGVFDRLRK
                     GKHLLKDEKLARLSPQEERILSLVADGRTNGQIGEELGLAEKTVKNYVSSILAKLEVA
                     RRAEAAAYLARHTTLPGA"
     gene            complement(394..1467)
                     /locus_tag="VFO47_00655"
     CDS             complement(394..1467)
                     /locus_tag="VFO47_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787239.1"
                     /translation="MLCPFDLQVWDVDRMLNFEIADDPHYEGLELQVFDDPVHGRGMI
                     VLFKRRHDGRFDVYRQPGLALDPAYVQVGGELGEWRQASIKPARFEIGPFGIDVDVRL
                     TDLAGREIRVRIDDRDGQRRHPGTLLAPVGSTIEHPVSLPLFLMGGCDLVRKTGPAFD
                     LRIGGRRVTTGRLPGGWLHRRRLVKYTAAPTAVVLNPAGDGPVATVDPRTPGNVRFDG
                     TSNRIESLQARTAGHRAGLVLEPALPDLARLRPGALATGGWRLDIDDSPAVVGGAWTV
                     ERRHDRVDVVLDVTHGWRPRGLPPLMAAVTRVAPVFRNWPMTYRWSATITLGDRPLLS
                     SRWERKSSRRDQSYRRLTTARPR"
     gene            complement(1678..1860)
                     /locus_tag="VFO47_00660"
     CDS             complement(1678..1860)
                     /locus_tag="VFO47_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787240.1"
                     /translation="MTHYLLSVHGVEGEVREPMTDEEMAQSHQQLGTLEQDLKSAGAW
                     VVSGRLHHPPPPLSCA"
     gene            complement(2078..2380)
                     /locus_tag="VFO47_00665"
     CDS             complement(2078..2380)
                     /locus_tag="VFO47_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET8787241.1"
                     /translation="MVTVGLLVRLVATPGKEAEVVSFLEGGLALVEEEPATIAWFAVR
                     LGPSEFGIFDAFPDDAGRQAHLGGQVAAALMAQAPELFAQPPVIEPVDVLAAKLPG"
BASE COUNT          441 a          958 c          913 g          386 t
ORIGIN      
        1 gccggcgccg ccggctatgt cctcaagcag atccgcggcg aggagctggt ccgggccatc
       61 caggccgtcg gcgccggtca gaacctgctg gacccggcgg tcaccaaggg cgtgttcgac
      121 cggctccgca agggcaagca cctcctcaag gacgagaagc tggcccgtct gtccccccag
      181 gaggaacgga ttctttcctt ggtggccgac ggccggacca acggccagat cggcgaggag
      241 ctcggcctgg ccgagaagac ggtcaagaac tacgtctcca gcatcctggc caagctcgag
      301 gtcgcccgcc gcgccgaagc cgccgcctac ctggcccgcc acaccaccct gcccggcgcc
      361 tgaccagccg gtcggtgtcg agggcggcgc gcgttatcgg ggtcgcgcgg tggtgagccg
      421 ccgatacgac tggtcgcgtc tgctgctctt gcgctcccac cgtgagctca gcagtggccg
      481 gtcgcccagg gtgatggtgg ctgaccagcg gtaggtcatc ggccagttgc gaaacacggg
      541 ggcgacccgg gtgacggccg ccatgagcgg cggcagcccc cgcggcctcc aaccatgggt
      601 gacgtcaagg acaacgtcga cgcggtcatg gcgccgctcg accgtccagg cgccgccgac
      661 gacggccggg ctgtcgtcga tgtccagccg ccagccaccg gtcgccaggg caccgggccg
      721 gagccgggca agatcgggga gggccggctc gagcaccaga ccggcacggt ggccggcggt
      781 tcgtgcctgg aggctttcga tccggttcga cgtaccgtcg aaccgcacgt tccccggtgt
      841 ccggggatcg acggtggcga cggggccgtc gccggccgga ttgaggacga ccgcggtggg
      901 ggcggcggtg tacttcacca gccgacgccg gtgcagccag ccgccgggga ggcgaccggt
      961 ggtcaccctc cggccaccga tgcggaggtc gaaggccgga ccggtcttac gcaccaggtc
     1021 acagccgccc atgaggaaca acggcagcga gaccgggtgc tcgatcgtcg agccgaccgg
     1081 cgcgagcagc gttcccgggt gccggcgctg tccatcccgg tcgtcgatcc gcacccggat
     1141 ctcccggccg gccaggtcgg tgaggcggac atcgacgtcg atcccaaacg ggccgatttc
     1201 gaaccgggcc ggcttgatgg acgcctggcg ccactccccc agctcaccac ccacctggac
     1261 gtatgcggga tccaaggcca ggccaggctg gcggtagacg tcgaagcgtc cgtcatggcg
     1321 ccgcttgaac aggacgatca tcccccggcc gtggacaggg tcgtcgaaca cctggagctc
     1381 caggccctcg tagtggggat cgtcggcgat ctcgaaattg agcatccggt cgacgtccca
     1441 cacctggagg tcgaaggggc acagcacccg ctgaccccca gtcccctggt ccggcttgtc
     1501 ggccccgaac gattcgttcc tgatgccagt cgtcgcaatc acctcccgaa gcccctccat
     1561 gcgagcgtgc cgccgcccag cgcgacaggg cagggtcaac ggtcccggcc gtcacgggcc
     1621 atccgcccat gccagcgccg tccgccgggg cggaagctgg aaccgacgaa cggagcctca
     1681 tgcgcacgac agtggcggtg gagggtggtg caggcgaccc gagacgaccc acgccccagc
     1741 cgacttcagg tcctgctcga gggtgccgag ctgctggtgg gactgggcca tctcctcgtc
     1801 ggtcattggc tcgcggacct cgccctcgac gccgtggacc gacaacaggt agtgcgtcat
     1861 ggtgaccctc ctgctggtgg gcgggcagct cccgccgctc accctgacta cgaacaggcc
     1921 ccgcccagat tgacataagg accgaaggaa ccctggtagc ggcatcgacc tcgatcacca
     1981 ggtcatgggt gtctctggtc ggatctaacc cgcccacttg ccggcacggt ccgatcggtt
     2041 gtcgctgcct gcccgtccgc ccagcttcga ccagcagtta cccaggcagc ttggccgcaa
     2101 ggacgtcgac cggctcgatg accggaggct gcgcgaacaa ctccggcgcc tgggccatca
     2161 atgctgccgc cacctggccg ccaaggtggg cttggcggcc cgcgtcgtcg ggaaaggcgt
     2221 cgaagatccc gaactccgag ggtccaaggc ggaccgcgaa ccaggcgatg gtcgccggtt
     2281 cctcctccac cagcgccaag cctccctcaa ggaagctgac cacctcagcc tccttgccgg
     2341 gcgtggccac cagacgaaca agtagtccca ctgtaaccat caccgctctc cttccaagga
     2401 gccgccgcca ccaagcaaac caaccatcaa gcatcatact gcgggggcgc acgcgctcgg
     2461 acagggagac gatgcggtac ggcgacggcg gagcaatcag cggacgacca ttgctcggga
     2521 cgccgatcgc cgctggcggc gacccaacat gaccagcgcg gcttggttgg cgtgtgccgg
     2581 ggatggtccg ttggtgtccc ctggcgcatg cccagatgtg ccccaggttg gcctccgcgt
     2641 taggtgtgtg gatcaccctg cccgggtgat gcgcactcgc cctggtcgag cgacgctt
//
LOCUS       DASSWR010000037         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_594867,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000037 DASSWR010000000
VERSION     DASSWR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594867"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFO47_00670"
     CDS             complement(<1..270)
                     /locus_tag="VFO47_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HET8787242.1"
                     /translation="MRVLVAGAGGQVGRALAEVYGGAKELVALDRTQLDITSWPAVAG
                     TVAELGPDLVVNAAAWTDVDGCEADPDHAFRANCLGPRHLALACRE"
     gene            complement(267..1262)
                     /gene="rfbB"
                     /locus_tag="VFO47_00675"
     CDS             complement(267..1262)
                     /gene="rfbB"
                     /locus_tag="VFO47_00675"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET8787243.1"
                     /translation="MRFLVTGGAGFIGSNYVRHLLATDPEARVTNFDKLTYAGNRASV
                     ADLEGDPRYSFVQGDICDPGQVAEAVAGHDVVVNFAAETHVDRSIVEPLEAVRTNTVG
                     VATLAEAARTAGVGRFLQVGTDEEYGTISDGSFAEDDRLEPSSPYSAGKAGGSLVALA
                     YAATHKLDVVVTRCTNNYGPYQFPEKVIPLFVTNLMDGHKVPLYGSGGNIRDWLYVLD
                     HCSAIDLVLRRGQTGAIYNIGAGNEVTNLELTRRILAAFGAGEDMIEFVADRPGHDWR
                     YSLDTTRVRELGWAPAHDFDQGLAETIAWYRSREDWWRPMKPAGATRTLDHGRAP"
     gene            complement(1308..2579)
                     /locus_tag="VFO47_00680"
     CDS             complement(1308..2579)
                     /locus_tag="VFO47_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET8787244.1"
                     /translation="MSSRRRRNLLRSLAILLVLLVVLTGAAAVYGYRYAGRLVAKGHR
                     PVANLTPAASGKAMNILLVGSDSRAGLSRRQLGRIQTEEVAGRRTDTIIVLHVSPDRD
                     KPVLVSLPRDLRSTVNGRTSKINAAYAFGGPDLLVKTVAETTGLPIHHYAEVDFAGFL
                     EVVDALGGITLCNRSGRRLDDPYANLHMAPGCQRMNGVKALAFVRARHVDSDFGRIGR
                     QQEFMRAVLAEVDRRSGLTSLPALKGVAEIATDHIKTDDTLGTGTALGLARRLRDLGP
                     ASLDMRVYPSVASPPRCAGCAAFVDPLPEAAILMRALARDAARLPAVGLPGGKGVSLA
                     TTRVTVLNGSGAQGAAARAAAGLRRLGVRVVGTGNAATPTGQASTLAYPPDLAQQARL
                     LSAVLGGQVRLVKAGEGGTLVLTVGSGFRLR"
     gene            2677..>3179
                     /locus_tag="VFO47_00685"
     CDS             2677..>3179
                     /locus_tag="VFO47_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HET8787245.1"
                     /translation="MNTSGAAFDSFNVAAERQIGRYGDKPLLTWYDDHRDERVELSYR
                     TFGNWVAKTANLLVDELGAGPGDRVGAVLPDHWRTPVVLAACWRAGVGVAALDPSSPD
                     PPPDGLVAAFVWEDHLAAVTDALGGIPVVAVGAELLGPGGHDLGQALSFARLVPSMGD
                     HFDGGADP"
BASE COUNT          391 a         1146 c         1212 g          430 t
ORIGIN      
        1 ctcgcggcag gccagggcca ggtggcgggg gccgaggcag ttggcccgga aggcgtggtc
       61 ggggtcggcc tcgcagccgt cgacgtcggt ccaggcggcc gcgttcacca ccaggtccgg
      121 cccgagctcg gccacggtcc cggccaccgc cggccagctg gtgatgtcga gctgcgtgcg
      181 gtcgagagcg accagctcct tggcgccgcc gtagacctcg gcgagcgccc gccccacctg
      241 cccaccggcg ccggcaacca gcaccctcac ggggcccgcc cgtggtccag ggtgcgggtg
      301 gccccggccg gcttcatcgg gcgccaccag tcctcgcggg agcggtacca ggcgatcgtc
      361 tcggccaggc cctggtcgaa gtcgtgggcc ggggcccagc cgagctcgcg cacccgggtc
      421 gtgtccagcg agtagcgcca gtcgtgcccg ggccggtcgg ccacgaactc gatcatgtcc
      481 tcgccggccc cgaaggcggc caggatgcgc cgggtcagct ccaggttggt gacctcgttg
      541 ccggccccga tgttgtagat ggcccccgtc tggccgcgcc gcagcaccag gtcgatggcg
      601 ctgcagtggt cgagcacgta cagccagtcg cggatgttgc cgccgctgcc gtacagcggc
      661 accttgtggc cgtccatcag gttggtcacg aacagcggga tgaccttctc ggggaactgg
      721 tagggcccgt agttgttggt gcagcgggtc acgaccacgt ccagcttgtg ggtggcggcg
      781 taggccaggg cgaccaggga gccgccggcc ttgccggccg agtagggcga cgacggctcc
      841 agccggtcgt cctcggcgaa ggagccgtcg ctgatggtgc cgtactcctc gtcggtcccg
      901 acctgcagga agcgcccgac cccggccgtg cgggccgcct cggccagggt ggccaccccg
      961 acggtgttgg tccgcaccgc ctccagcggc tccacgatcg agcggtccac gtgggtctcg
     1021 gcggcgaagt tgaccaccac gtcgtggccg gccacggcct cggccacctg gccagggtcg
     1081 cagatgtcgc cctgcacgaa gctgtaccgg gggtcgccct ccaggtcggc cacgctcgcc
     1141 cggttgccgg cgtaggtgag cttgtcgaag ttggtcaccc gcgcctcggg gtcggtggcg
     1201 agcaggtggc ggacgtagtt ggagccgatg aagccggccc cgccggtcac gaggaagcgc
     1261 atggcggcag ctccgctcct tggtcggtgc gatgggcggc caagagccta ccggagcctg
     1321 aacccggacc ccacggtcag caccagcgtc ccgccctcgc cggccttgac cagcctgacc
     1381 tggccgccga gcacggccga gagcagccgg gcctgctggg ccaggtccgg cgggtaggcc
     1441 agggtcgagg cctggccggt cggggtggcg gcgttgccgg tgccgacgac ccggacgccg
     1501 aggcggcgca ggccggcggc ggccctggcc gcggccccct gggcgccgct gccgttgagg
     1561 accgtgaccc gggtggtggc cagcgacacc cccttgccgc cgggcaggcc gaccgcgggc
     1621 agccgggcgg cgtcgcgggc cagggcccgc atgaggatgg ccgcctcggg cagcgggtcg
     1681 acgaaggcgg cgcagccggc gcagcggggc ggcgaggcca cgctcgggta gacgcgcatg
     1741 tcgagcgagg ccgggccgag gtcgcgcagc cgcctggcca gcccgagggc cgtgcccgtg
     1801 cccagggtgt cgtcggtctt gatgtggtcg gtggcgatct cggccacgcc cttgagggcg
     1861 ggcaggctcg tcaggccgct gcgccggtcc acctcggcca gcacggcccg catgaactcc
     1921 tgctggcggc cgatgcggcc gaagtcggag tcgacgtgcc tggcccgcac gaaggcgagc
     1981 gccttgaccc cgttcatccg ctggcagccc ggcgccatgt gcaggttggc gtacgggtcg
     2041 tccagccgcc ggccgctgcg gttgcacagg gtgatgccgc cgagggcgtc gaccacctcg
     2101 aggaagccgg cgaagtcgac ctcggcgtag tggtggatgg gcagcccggt cgtctccgcc
     2161 accgtcttga ccagcaggtc ggggccgccg aaggcgtagg cggcgttgat cttgctggtc
     2221 cgcccgttga cggtggagcg gagatcacgc gggagcgaga ccagcaccgg cttgtcccgg
     2281 tccgggctga cgtggagcac gatgatggtg tcggtgcgcc ggccggccac ctcctcggtc
     2341 tggatccggc cgagctggcg gcggctcagg ccggcccgcg agtcggaccc gacgagcagg
     2401 atgttcatcg ccttgccgga ggcggccggg gtcaggttgg ccaccggccg gtggcccttg
     2461 gcgaccagcc ggccggcgta gcggtagccg tacacggcgg ccgccccggt cagcacgacc
     2521 aggagcacca gcaggatggc caggctccgg agcaggttgc gccgacggcg cgagctcatg
     2581 ggctgcggtc ggtcacggtg cgggcgtgct cctgctcatg gctgcgcggg cggcgcaatt
     2641 ggccttggga tttgaagaag cctagcatgc gcccccgtga acacctcggg cgccgcgttc
     2701 gacagcttca acgtcgccgc cgagcgccag atcgggcgct acggcgacaa gccgttgctc
     2761 acctggtacg acgaccaccg ggacgagcgg gtcgagctgt cctaccgcac cttcggcaac
     2821 tgggtcgcca agacggccaa cctgctggtc gacgagctcg gcgccgggcc cggggaccgg
     2881 gtcggggccg tcctccccga ccactggcgg accccggtcg tccttgccgc ctgctggcgg
     2941 gccggcgtgg gcgtggccgc cctcgacccg tcgtcccccg acccgccgcc cgacggcctg
     3001 gtcgccgcgt tcgtgtggga ggaccacctg gccgccgtca ccgacgccct cggcgggatc
     3061 ccggtggtcg ccgtcggcgc cgagctgctc ggccccggcg gccacgacct gggccaggcc
     3121 ctcagcttcg cccggctcgt ccccagcatg ggggaccact tcgacggcgg cgccgaccc
//
LOCUS       DASSWR010000038         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_651286,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000038 DASSWR010000000
VERSION     DASSWR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651286"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFO47_00690"
     CDS             <1..193
                     /locus_tag="VFO47_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787246.1"
                     /translation="APFAAGAGVVLVGGLDPGRFWKRVADERVTVAVLDPAQAAAVRD
                     AGPPPAGLDHSRLRMLLGD"
     gene            253..1746
                     /locus_tag="VFO47_00695"
     CDS             253..1746
                     /locus_tag="VFO47_00695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8787247.1"
                     /translation="MKLSVVVPFYNELRTLPTVIDRLLRVDFDALGLETELIFVDDGS
                     TDAGRSLLDPLPRDDVRVIVHPANRGKGAAVKTGLEAATGDILCIQDADLEYDPRDLP
                     ALIEPILDGDYEVVYGSRFRGSAAGLYYTHRIANRLLNLMVNVAFNRYLSDVYTGYKV
                     FTRHAYRGLRLTAKSFTVEMELTAHFLRKGLIIYEVPISYRARTYAEGKKIHFKDGFL
                     AAGAVARYRFRRPPERTGPAAYGAPDDPGVMRAVEGGATIHTLGLEDLATAHRYRRYL
                     HGLLAPYLGPSVLEVGAGLGDFSAQLLDRDRLVVNDNDPICVRALDERFGERPGIEVL
                     PADVLKLKVEPPVDSVVALNVLERMDDDVAALRAMARAALPGGRVLLVVPAYPSLAGA
                     FDDALGHLRRYTPGSLGDTVETAGLVPEVIRPVNLLGGLAWWAAVRVGRQARPTPALV
                     RLYDRLVVPAERLLERRLQPRFGQSILCVAQVPEPENQPQTRRERFA"
     gene            complement(1915..2673)
                     /locus_tag="VFO47_00700"
     CDS             complement(1915..2673)
                     /locus_tag="VFO47_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8787248.1"
                     /translation="MPADPSQPKVVVVMPAYNAARTLVRTYRDIPPGVVDHVILVDDL
                     SRDDTVAVAELLNLQVVVHRQNLGYGGNQKTCYDTALAEGADVVVMLHPDYQYDATRI
                     PDLVAPILAGDADMVLGSRFLGDPLAGGMPRWKFVANRFLTTLQNRAYDLRLSEYHTG
                     FRAYSRKLLETIDYRANSNDFVFDQELVGQVVRAGFRIAEIPVPTRYFAEASSVGFRR
                     SVVYGLSTLRTLARHRGATRSSAGSANSPLTGEG"
     gene            complement(2684..>3215)
                     /locus_tag="VFO47_00705"
     CDS             complement(2684..>3215)
                     /locus_tag="VFO47_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787249.1"
                     /translation="LAALVGRGRAGDRDRSGGTGRLAAVAGIGLGLAVALPGVVTYLD
                     GALDSGRSREQGQRALSASLPSSAVVYGAYAPTMLFDTQLVTRTPWPTAEANVHDPVG
                     RLGVTHLLVGAEPADPTGRVATLAGRGAGVTLARVRWGPGELVLYGLPATSGVGVSPL
                     AAPIDYHPLAGPSRRM"
BASE COUNT          423 a         1222 c         1149 g          421 t
ORIGIN      
        1 ggccccgttc gccgccggcg ccggggtggt gctggtcggc ggcctcgacc ctggccggtt
       61 ctggaagcgg gtcgccgacg agcgggtcac ggtggccgtg ctcgaccccg cccaggccgc
      121 cgccgtccgc gacgccggac cgccaccggc cggcctcgac cattcccggc tgcggatgct
      181 gctcggcgac tgaccgctca agcgacccga cgtggtactt tgccggctgc tgccgaaggg
      241 ggagaaatgt ccatgaagct gtcggtggtc gtcccgttct acaacgaact gcggacgctc
      301 ccgacggtca tcgaccgcct cctcagggtc gacttcgacg ccctcggcct ggagacggag
      361 ctgatcttcg tcgacgacgg ctccaccgac gccgggcgga gcctgctcga cccgctgccc
      421 cgcgacgacg tccgggtcat cgtccacccc gccaaccggg gcaagggcgc cgccgtcaag
      481 accggcctgg aggcggccac cggcgacatc ctctgcatcc aggacgccga cctcgagtac
      541 gacccccgcg acctgccggc cctgatcgag ccgatcctgg acggcgacta cgaggtcgtc
      601 tacggcagcc gcttccgggg cagcgcggcc ggcctctact acacccaccg gatcgccaac
      661 cggctgctca acctgatggt caacgtggcc ttcaaccgct acctgtcgga cgtctacacc
      721 ggttacaagg tgttcacccg ccacgcgtac cgggggctgc ggctgaccgc caagagcttc
      781 accgtggaga tggagctgac cgcccacttc ctgcgcaagg gcctgatcat ctacgaggtg
      841 ccgatctcgt accgggcccg cacctacgcc gagggcaaga agatccactt caaggacggc
      901 ttcctggccg ccggggcggt ggcccgctac cgctttcgcc gcccgccgga gcggaccggg
      961 ccggccgcct acggcgcccc cgacgacccg ggggtgatgc gcgccgtcga gggcggggcc
     1021 accatccaca ccctcggcct ggaggacctg gccacggccc accgctaccg ccgctacctg
     1081 cacggcctgc tcgcccccta cctgggcccg tccgtgctcg aggtcggggc cggccttggc
     1141 gacttctcgg cccagctgct ggaccgcgac cgcctggtcg tcaacgacaa cgacccgatc
     1201 tgcgtgcgcg ccctcgacga gcgcttcggc gagcgccccg gcatcgaggt cctgcccgcc
     1261 gacgtgctca agctcaaggt cgagccgccg gtcgactcgg tggtcgccct caacgtcctg
     1321 gagcgcatgg acgacgacgt ggccgccctc cgggccatgg cccgggccgc cctccccggc
     1381 ggccgcgtcc tgctggtcgt gcccgcctac ccgtcgctgg ccggcgcctt cgacgacgcc
     1441 ctcggccacc tgcgccgcta cacccccggc agcctcggcg acaccgtcga gaccgccggc
     1501 ctggtccccg aggtcatccg cccggtcaac ctcctcggcg gcctcgcctg gtgggccgcc
     1561 gtccgggtcg gccgccaggc ccgccccacc cccgccctgg tccggctcta cgaccgcctg
     1621 gtggtccccg ccgagcgcct cctggagcgc cgcctccagc cccgcttcgg ccagtccatc
     1681 ctctgcgtgg cccaggtccc cgagcccgag aaccagcccc agacccgccg cgagcgcttc
     1741 gcctgaccgc ggccgaccct cgctcaccgg gctcgcgacc cccaccggaa ccggaacggc
     1801 cagcgcgggc gtctggcgcc aggcgcccgc acccggcgct cctggcccag caggtacgcc
     1861 gtccgaggca ccaggagctg caccctcctc cacagctccc cgcggttgct ccgatcagcc
     1921 ctcgccggtg agcgggctgt tggccgaacc cgcgctgctc cgggtggcgc cgcggtggcg
     1981 ggccagggtg cggagggtgg agaggccgta gacgaccgag cggcggaacc cgaccgagct
     2041 ggcctcggcg aagtagcggg tggggacggg gatctcggcg atacggaagc cggcccggac
     2101 gacctggccg accagctcct ggtcgaacac gaagtcgttg gagttggccc ggtagtcgat
     2161 cgtctccagc agcttgcggg agtaggcccg gaagccggtg tggtactcgg acaggcgcag
     2221 gtcgtaggcg cggttctgga gggtggtcag gaaccggttg gccacgaact tccagcgggg
     2281 catgccgccg gccagcgggt cgccgaggaa gcggctgccc agcaccatgt cggcgtcgcc
     2341 ggccaggatg ggggcgacca ggtcggggat gcgggtggcg tcgtactggt agtcggggtg
     2401 gagcatgacg accacgtcgg cgccctcggc cagggcggtg tcgtagcagg tcttctggtt
     2461 gccgccgtag ccgaggttct ggcggtggac gacgacctgg aggttcagga gctcggccac
     2521 ggccacggtg tcgtcgcggg acaggtcgtc gaccaggatg acgtggtcga ccacgcccgg
     2581 ggggatgtcg cggtaggtgc ggaccagcgt ccgggcggcg ttgtaggccg gcatcaccac
     2641 gacgaccttg ggctgggacg ggtctgcggg catcggcacc tacctacatc cggcgggacg
     2701 ggcctgccag cggatggtag tcaatcggcg cggccagggg cgagacgccg acgccggacg
     2761 tggccggcag gccgtacagg accagctcgc cgggacccca gcggacccgg gccagggtca
     2821 cgccggcccc gcggccggcc agggtcgcca cccggccggt cgggtcggcg ggctcggcgc
     2881 cgacgaggag gtgggtgacg ccgaggcggc cgaccgggtc atggacgttg gcctcggcgg
     2941 ttggccaggg ggtgcgtgtg accagctggg tgtcgaacag catggtcggg gcgtaggcgc
     3001 cgtacacgac cgcgctgctg ggcagcgacg cggacagggc ccgctggccc tgctcgcgcg
     3061 agcgcccgct gtccagcgcc ccgtccaggt acgtcaccac cccgggcagg gccacggcca
     3121 gcccgaggcc gatcccggcc accgcggcca gccgccctgt tccgccggac cggtcccggt
     3181 cccccgcccg gccccggccg acgagggcgg ccagg
//
LOCUS       DASSWR010000039         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1160067, whole genome shotgun sequence.
ACCESSION   DASSWR010000039 DASSWR010000000
VERSION     DASSWR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160067"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFO47_00710"
     CDS             <1..875
                     /locus_tag="VFO47_00710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8787250.1"
                     /translation="RPGPAEVGTVTRLESDYYSYMEFDPEHTREVLRHYVPMFEDKSP
                     VLELGCGRGEFLGLLDEKGVKGKGVDSDEGMVEAAVAKGLDVIRGDAMAFLNGEPAPG
                     PYQGVFCAHFIEHLTPDQVRELLAGVRRVLAPGGRFVAVTPNPACYSVLSHDFWRDPT
                     HVRFYDLPLLEFLCRQAGLEVEATGTNPANHPGPPPEYLVPEPVVHPPLDDLVERAMG
                     KLGASLDHRDRKGRVTEHQHDAEWAYELAHVVKVLSGRLQETTEALREVRRAHDKLVW
                     GLYQSNETYVVARG"
     gene            910..>2173
                     /locus_tag="VFO47_00715"
     CDS             910..>2173
                     /locus_tag="VFO47_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HET8787251.1"
                     /translation="MKVAVYNRFLQSMGGGERHSSMLAQVLADDGHEVDLVGHKDVGK
                     EILADHLGLNLGKVNLRIVPERGEPQVARLSAEYELFVNASYMSRVKAQAARNLYLCY
                     FPTPFDHDLAGWQRRLVRLLGPHVRQGKPGVVEWGLGWFPPEGGRRRTWVWTSGRAAL
                     QLAGGPAKRVVFDLGRPGAPGPAEVVISHEGGIELARLEASPAGFRRHELDLPPSEGD
                     REVVFDSGTFVPGAGDDRTLGVAVSRLHMADATWEPRRWAGERFPWLLRDPTDLEFLG
                     HYERVLANSQYTRHWIRRLWGVDSDVLFPPIRTKDLHPGPKTRRILTVGRFIAPGFGH
                     SKKQLEQVQAFGEMVRGGGLDGWELHLVGGCEPAHRPYLAEVERAAGDLPVRIHANAP
                     RALVEELFATSSVFWVATGLGEDEEAAPW"
BASE COUNT          301 a          797 c          783 g          292 t
ORIGIN      
        1 ggcgcccagg cccggcggag gtcggcacgg tgacccggtt ggagagcgac tactactcgt
       61 acatggagtt cgaccccgag cacacgcgcg aggtcctgcg ccattacgtg cccatgttcg
      121 aggacaagag ccccgtgctg gagctgggct gcgggcgcgg cgagttcctc gggctcctgg
      181 acgagaaggg cgtcaagggc aagggggtcg actccgacga gggcatggtc gaggcggccg
      241 tggccaaggg cctggacgtc atccgcggcg acgccatggc cttcctgaac ggcgagcccg
      301 cccccgggcc ctaccagggg gtgttctgcg cccacttcat cgagcacctc acccccgacc
      361 aggtgcgcga gctgctggcc ggggtccggc gggtgctggc ccccgggggc cggttcgtcg
      421 ccgtgacccc gaacccggcc tgctactcgg tcctcagcca cgacttctgg cgcgacccga
      481 cccacgtccg cttctacgac ctgcccctgc tcgagttcct ctgccgccag gccggcctgg
      541 aggtggaggc caccggcacc aacccggcca accaccctgg cccgccgccg gagtaccttg
      601 tccctgagcc agtcgtccac ccacccctgg acgacctggt cgagcgggcc atggggaagc
      661 tcggggccag cctcgaccac cgcgaccgca agggccgggt cacagagcac cagcacgacg
      721 cggagtgggc gtacgagctg gcccatgtgg tcaaggtgct gtccggccgg ctccaggaga
      781 cgaccgaggc cctccgggag gtccgcaggg cccacgacaa gctggtgtgg gggctgtacc
      841 agagcaacga gacctacgtc gtcgcccggg gctgaccctg ggcccggaga accagggagc
      901 tagcgcgttt tgaaggtggc ggtctacaac cggttcctgc agtccatggg tgggggagag
      961 cgccacagca gcatgctcgc ccaggtcctg gccgacgacg gccacgaggt cgacctggtc
     1021 ggccacaagg acgtcggcaa ggagatcctc gccgaccacc tcggcctcaa cctgggcaag
     1081 gtcaatctcc ggatcgtccc cgagcggggg gagccccagg tcgcccgcct gtcggccgag
     1141 tacgagctgt tcgtcaacgc ctcctacatg agccgggtca aggcccaggc cgcccgcaac
     1201 ctgtacctct gctacttccc gacccccttc gaccacgacc tggccggctg gcagcggcgc
     1261 ctggtccggc tgctcggccc ccacgtccgc cagggcaagc cgggggtggt cgagtggggg
     1321 ctgggctggt tcccgcccga agggggccgg cgccggacct gggtgtggac cagcggccgg
     1381 gcggcgctcc agctggccgg cggtccggcc aagcgggtcg tgttcgacct cggccgcccc
     1441 ggcgcccccg gcccggccga ggtggtcatc agccacgagg gcgggatcga gctggcccgg
     1501 ctggaggcct ccccggccgg cttccgccgc cacgagctgg acctgccgcc gagcgagggc
     1561 gaccgcgagg tcgtcttcga cagcggcacc ttcgtccccg gggccggcga cgaccgcacc
     1621 ctcggggtgg cggtgagccg gctgcacatg gccgacgcca cctgggagcc gcggcgctgg
     1681 gccggcgagc gcttcccgtg gctgctgcgc gaccccaccg acctggagtt cctcggccat
     1741 tacgagcggg tgctggccaa ctcccagtac acccggcact ggatccggcg gctgtggggg
     1801 gtcgactccg acgtgctgtt tcccccgatc cggacgaagg acctgcaccc gggccccaag
     1861 acccggcgca tcctgaccgt tggccgcttc atcgcccccg gcttcggcca ctccaagaag
     1921 cagctcgagc aggtccaggc gttcggcgag atggtccgcg gcggcgggct ggacggctgg
     1981 gagctgcacc tggtcggcgg ttgcgagccg gcccaccggc cctacctggc cgaggtcgag
     2041 cgggccgccg gggatctgcc ggtgcgcatc cacgccaacg ccccccgggc cctggtcgag
     2101 gagctgttcg ccaccagctc ggtcttctgg gtggccaccg ggctgggcga ggacgaggag
     2161 gccgcgccct ggg
//
LOCUS       DASSWR010000040         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_340419,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000040 DASSWR010000000
VERSION     DASSWR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340419"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFO47_00720"
     CDS             complement(<1..509)
                     /locus_tag="VFO47_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8787252.1"
                     /translation="MDLATGLETLLVAAAVAAIAPVLVAWLPEPRIPQVVVLLAGGVL
                     VGPQVLGWADRPAIDLLANVGLGFLFLLAGYELDLALFRVRPGRLAMVAWLVTVALAA
                     ATVGLLEAAGLVRAFVPVALGLTTTALGTLLPILRDNDMLGGRFGRYLIAAGAVGELF
                     PIFAVALFLG"
     gene            599..1954
                     /locus_tag="VFO47_00725"
     CDS             599..1954
                     /locus_tag="VFO47_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HET8787253.1"
                     /translation="MAQARAAAGRALRKAVPRSSHAAWDPRGRPSPLAVLEETNRQRL
                     PDLVPIRDARMRASPFAFYRGAPAVMARDLATTPVTGITVQACGDAHLLNFGLFATPE
                     RNLSFGLNDFDETLPGPWEWDVKRLATSFVVAARTVGFDAGLGREAALATVRTYREQM
                     ARYAGMRLLDVWYSRVDAAAIVAMAKGRRRQLVAARLAKAEHHTSLNAMPRLTEPQGE
                     GRRFVEDPPLLTHVAECDERWVTEVLVRYRSSLSDERRGLLERFRPVDAARKVVGVGS
                     VGTRCYVVLLLGERHDDPLLLQVKQATASVLEPYAGRSRYRHPGHRVVNGQRLLQTAS
                     DIFLGWTGDDVADYYIRQLWDMKGGIHLETLDPADLVPYGRLCGWVLARAHARSGDPA
                     AIAGYLGSGDAFDQAVASFAEAYADQTEADHAAFSAARDSSSGDGGRGGGTNQRKGAE
                     G"
     gene            complement(2137..3540)
                     /locus_tag="VFO47_00730"
     CDS             complement(2137..3540)
                     /locus_tag="VFO47_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8787254.1"
                     /translation="MDLGAYLDAARAFDAGFRSRWEPVPAAAASEVDGERLAAAWAEY
                     TGRLQDNYPFFHPRYAGQMLKPPHPVAIAGYLAAMTVNPNNHALDGGPATGAMEKEVV
                     ADLAAMFGLPSGGLGHLTSSGTIANLEALWVARQLAPGGKVAHSQEAHYTHARMCEVL
                     GVESVPVTADAAGRMDLDALEELLATGEVGTVVLTAGTTGLGAVDPIAEALELRDRYG
                     CRLHVDAAYGGFFALLAWDADGAELLGPETAARLRAVGECDSVVVDPHKHGLQPYGCG
                     AVLFRDPTVGRLYRHDSPYTYFTSDELHLGEISLECSRAGAAAGALWLTLRALPLRAA
                     DGLGPVVAGGLRAARGWADLLEASDELHLYQRPELDILAFWPDPGSPSCAAVDAASAA
                     LLHAAMDDPSPVYLSTLRLAAGRLRARDPSLAADVPEARVLRSVLMKPEHEAYVETLH
                     HEVVRVARSLSGDRAAG"
BASE COUNT          511 a         1428 c         1429 g          447 t
ORIGIN      
        1 ccgaggaaca gggcgacggc gaagatcggg aacagctccc ccaccgcccc ggcggcaatc
       61 aggtagcggc cgaagcggcc accgagcatg tcgttgtcgc gcaggatggg cagcagcgtc
      121 cccagggcgg tggtggtcag cccgagcgcg accgggacga aggcgcgaac cagcccggcc
      181 gcttccagga ggcccaccgt ggcggcggcc aacgccaccg tcaccagcca ggccaccatg
      241 gccaggcgcc ccgggcgcac ccggaacagc gcgagatcga gctcgtagcc ggccagcagg
      301 aacaggaacc cgagcccgac gttggccagc aggtcgatgg cgggccggtc ggcccagccg
      361 agcacctgcg ggccgaccag caccccgccg gccagcagca ccaccacctg ggggatgcgg
      421 ggctcgggca gccaggccac cagcacgggg gcgatcgcgg ccaccgcggc cgccaccagc
      481 agcgtctcca ggcccgtggc aagatccatg cgcgacctcc ggcctcgacc tcgtcgtcga
      541 gcatggcatg gcggatactg gtcccgcagc cggacggcgg tattggggga tacggcggat
      601 ggcgcaagct cgagcggccg ccggccgcgc cctgcgcaag gcggtgccac gctccagcca
      661 cgcggcctgg gacccgcgcg ggcggccgtc gccgctggcc gtgctggagg agaccaaccg
      721 ccagcgcctg cccgacctgg tcccgatccg cgacgcccgc atgcgggcgt cgccgttcgc
      781 gttctaccgg ggcgcccccg cggtgatggc ccgcgacctg gccaccaccc ccgtcaccgg
      841 catcaccgtg caggcctgcg gggacgccca cctgctcaac ttcgggctgt tcgccacccc
      901 ggagcggaac ctgtcgttcg gcctcaacga cttcgacgag acgctccccg gcccctggga
      961 gtgggacgtc aagcggctgg cgaccagctt cgtggtggcc gcccgcacgg tcgggttcga
     1021 cgccggactc ggccgggagg cggccctggc cacggtccgc acctaccgcg agcagatggc
     1081 ccgctacgcc ggcatgcggc tgctggacgt ctggtactcg cgggtcgacg cggccgcgat
     1141 cgtggccatg gccaaggggc ggcgccggca gctggtggcc gcccgcctgg ccaaggccga
     1201 gcaccacacc agcctgaacg cgatgccccg gctgaccgag ccgcagggtg agggccggcg
     1261 gttcgtggag gacccgccgc tgctcaccca cgtcgccgag tgcgacgagc ggtgggtcac
     1321 cgaggtgctg gtccgctacc gctccagcct gtcggacgag cggcgcgggc tgctcgaacg
     1381 cttccggccg gtggacgccg cccgcaaggt ggtcggggtc ggcagcgtcg gcacccgctg
     1441 ctatgtggtg ctgctgctcg gagagcgcca cgacgacccg ctgctgctcc aggtcaagca
     1501 ggccaccgcc tcggtgctgg agccgtacgc cgggcgcagc cgctaccgcc atcccggcca
     1561 ccgggtcgtc aacggccagc ggctgctcca gacggccagc gacatcttcc tcggctggac
     1621 cggcgacgac gtggccgact actacatccg ccagctctgg gacatgaagg gcgggatcca
     1681 cctggagacg ctggatccgg ccgacctggt cccctacggc cgcctctgcg gctgggtgct
     1741 ggccagggcc cacgcccgca gcggcgaccc ggccgcgatc gccggctacc tgggctccgg
     1801 cgacgccttc gaccaggcgg tggcgtcctt cgccgaggcc tacgccgacc agaccgaggc
     1861 cgaccacgcg gcattctcgg ccgcccggga ttcgtcctcc ggggacgggg gtaggggtgg
     1921 cggcacgaac caacggaaag gagccgaagg atgatcctct cgatcctggt cacgttggcg
     1981 ttcgtggcca ccctggcgtt catgctcgtc cgcgccttca ccggcccggt ccggcaccgc
     2041 cgcagccgcc cggtgcactg acgggaccac ccagggcagg cggtgaagct gctggggcgg
     2101 cgcctgcggt ctcgctctcg gtgacctgcg ggatgctcag cccgctgccc ggtccccgct
     2161 gagcgaccgc gcgacccgca ccacctcgtg gtggagggtc tccacgtagg cctcgtgctc
     2221 gggcttcatc aggacgctgc gcaggacccg ggcctcgggg acgtcggcgg ccagggacgg
     2281 gtcgcgggcg cggaggcggc cggcggccag gcgcagggtg ctgaggtaga ccggggaagg
     2341 gtcgtccatg gccgcgtgca ggagggcggc cgaggcggcg tcgacggccg cgcaggaggg
     2401 tgaccccggg tcgggccaga aggcgaggat gtcgagctcg ggccgctggt acaggtgcag
     2461 ctcgtcgctc gcctccagca ggtcggccca gcccctggcg gcgcggaggc cgccggccac
     2521 gaccgggccg aggccgtcgg cggcccgcag gggcagggcc cgcagggtca gccagagagc
     2581 gccggcggcc gccccggccc gggagcactc caggctgatc tcgcccaggt gcagctcgtc
     2641 ggaggtgaag taggtgtagg gcgagtcgtg gcggtacagc cggcccaccg tcgggtcgcg
     2701 gaacagcacc gccccgcagc cgtacggctg caggccgtgc ttgtgggggt cgaccacgac
     2761 cgagtcgcac tcccccaccg cccgcagccg cgccgccgtc tccggcccca gcagctcggc
     2821 cccgtcagcg tcccaggcca gcagggcgaa gaacccgccg taggcggcgt cgacgtgcag
     2881 ccggcaaccg taccggtcgc gcagctcgag cgcctcggcg atcgggtcga ccgcccccag
     2941 tccggtggtg ccggcggtca gcaccaccgt ccccacctcc ccggtggcca gcagctcctc
     3001 gagagcgtcc aggtccatgc gcccggccgc gtcggccgtc accggcacgc tctccacccc
     3061 caggacctcg cacatgcggg cgtgggtgta gtgggcctcc tggctgtggg cgaccttgcc
     3121 gccgggggcg agctggcggg ccacccacag cgcctccagg ttggcgatgg tgccgctgga
     3181 ggtgaggtgc ccgaggccgc cggacgggag gccgaacatg gccgccaggt cggcgacgac
     3241 ctccttctcc atcgccccgg tggccgggcc gccgtccagg gcgtggttgt tggggttgac
     3301 ggtcatcgcg gccaggtagc cggcgatcgc caccgggtgc ggcggcttga gcatctggcc
     3361 ggcgtagcgg gggtggaaga acgggtagtt gtcctgcagc cgcccggtgt actcggccca
     3421 ggcggcggcc agccgctcgc cgtccacctc cgacgccgcg gccgcgggca ccggctccca
     3481 gcgggaccgg aagccggcgt cgaaggcgcg ggccgcgtcc aggtaggcgc ccaggtccat
     3541 gtcacccctc cgtggccgga tcgagacccg gccacgttcg gtccgtgacc gaccggcccc
     3601 aggatgctac ccggcgcgac cagcacacac gcggccgggc cggcggcacc aggcgcacga
     3661 gggggcgcct ggtgccgcct tttctccggc cggtgctagc gagccgtcgc gcggtggcgt
     3721 ccgcggtagg ccgtcacggc gaccagcgcg gtcccggcga ggatcaggcc ggcggccagc
     3781 ccggtcaggg tcggcgcccg ggcgggcccg gtgaa
//
LOCUS       DASSWR010000041         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_707783,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000041 DASSWR010000000
VERSION     DASSWR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707783"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFO47_00735"
     CDS             complement(<1..395)
                     /locus_tag="VFO47_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8787255.1"
                     /translation="MTEQTGQGPEPAHERASATQVGARGPEGLPDEVGADPGQAGRAS
                     AGIGRFETHVSVLPAFDDHHPPEQELIDDCVHCGFCLPTCPTYVLWGEEMDSPRGRIY
                     LMELGRQGEALNTEMVRHFDQCLGCMACVT"
     gene            complement(395..1663)
                     /locus_tag="VFO47_00740"
     CDS             complement(395..1663)
                     /locus_tag="VFO47_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8787256.1"
                     /translation="MGLQELARGDLVQPAPPGTAVDGVPVARVARPVSVEEASQVLRA
                     AAADGLAVAFAGGGSKLGLGNPPQRADLLVSTERLDQVLEHAAGDLVVRAQAGLRLAD
                     LQATLAPAGQWLALDPPEPGATLGGVVAANASGPRRLRYGTVRDLIIGITVVLADGTV
                     ARAGGKVVKNVAGYDLAKLVCGSFGTLGLVAEVIFRLHPVPAAASVVTFRAEAPERVG
                     QAVQRLLRSALEPSAIELQLDEWGWPGRLTTVFEGIGPGVEAQAAAAVELLGQIGEAA
                     VAGPGETDAALHQLGALPFEKAELAFKATFPPAELPGLLAGGVLGRDGQLRVHAGTGV
                     AWLASSAREGDLRADHPAVPRVVAWVAETRERLAAVGGGLVVVKAPAHLKRELDVWGP
                     PGDALGLMRRVKERFDPGRRMSPGRFVGGI"
     gene            complement(1667..>2665)
                     /locus_tag="VFO47_00745"
     CDS             complement(1667..>2665)
                     /locus_tag="VFO47_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8787257.1"
                     /translation="CLKYGFTTNHVTGLEVVLPDGELVHLGGGKAPDPPGYDLLGAFV
                     GSEGTLGITTKVTLRLLRKPEAVRTLLAAFKGTDQAGDAVSRIIGAGIVPAAIEMMDA
                     LAMEAAEAAVHVHYPEGAGAVLVVELDGPAAEVEHQFDEVERHCQEAGSTTIQIAEDD
                     DQRALFWKGRKSAFAAVGRISPDYYVQDGVIPRTALPEVLRRITELADERGLRVANVF
                     HAGDGNLHPLVLYDGEVSGQEQRAEELAAEILYTCLEHGGSITGEHGVGFDKKQYMGR
                     QFTPEDLDTMQLVRCAFDPEGRANPGKVFPTPRLCGERPGPRRVHPAEQAGLAEVF"
BASE COUNT          322 a         1032 c          976 g          335 t
ORIGIN      
        1 gtgacgcagg ccatgcagcc gaggcactgg tcgaagtggc ggaccatctc ggtgttcagg
       61 gcctcgccct ggcgtcccag ctccatcagg tagatgcggc cccggggcga gtccatctcc
      121 tcgccccaga gtacgtaggt cgggcaggtg ggcaggcaga agccgcagtg gacgcagtcg
      181 tcgatcagct cctgctcggg cgggtggtgg tcgtcgaagg cgggcagcac cgacacgtgg
      241 gtctcgaacc gcccgatccc cgcgctcgcc cggccggcct ggcccgggtc ggccccgacc
      301 tcgtcgggca gcccctccgg ccctcgcgcc ccgacctggg tcgccgaggc ccgctcgtgg
      361 gccggctcgg gcccctgtcc cgtctgctcg gtcatcagat gcctcccacg aaccggcccg
      421 ggctcatccg gcggccaggg tcgaaccgct cctttacccg gcgcatcagc ccgagcgcgt
      481 ccccgggcgg cccccacacg tccagctccc gcttgaggtg cgccggcgcc ttgaccacga
      541 ccaggccgcc cccgacggcg gccagccgct ccctggtctc ggcgacccac gcgaccaccc
      601 tcgggaccgc cgggtggtcg gcccgcaggt cgccctcgcg ggccgagctg gccagccagg
      661 ccacgccggt gccggcgtgc accctgagct ggccgtcccg gccaaggacg ccgccggcca
      721 ggaggccggg cagctcggcc ggcgggaagg tggccttgaa cgcgagctcc gccttctcga
      781 acgggagcgc gccgagctgg tggagggcgg cgtcggtctc ccccggcccg gccacggccg
      841 cctccccgat ctggccgagg agctcgacgg cggccgccgc ctgcgcctcc acccccggcc
      901 cgatgccctc gaacacggtc gtgagccggc ccggccagcc ccactcgtcc agctgcagct
      961 cgatcgcgct cggctcgagg gcggagcgga gcagccgctg gacggcctgg ccgacccgct
     1021 cgggcgcctc cgcccggaag gtcaccacgg acgcggccgc cggcacgggg tggaggcgga
     1081 agatgacctc ggcgaccagg ccgagggtgc cgaacgagcc gcagaccagc ttggccaggt
     1141 cgtagccggc gacgttcttg accaccttgc cgcccgccct ggccacggtg ccgtcggcca
     1201 ggaccacggt gatgccgatg atcaggtcgc gcaccgtgcc gtagcgcagc cgccgcgggc
     1261 ccgaggcgtt ggccgccacc accccgccga gggtggcccc cggctcgggc gggtcgaggg
     1321 ccagccactg gccggccggg gccagggtgg cctggaggtc ggccaggcgc aggcccgcct
     1381 gggcccggac gaccaggtcg ccggcggcgt gctccagcac ctggtcgagc cgctcggtgg
     1441 agacgagcag gtcggcccgc tggggcgggt tccccagccc gagcttgctc cccccgcccg
     1501 cgaaggccac ggccaggccg tcggcggcgg cggcccggag gacctggctg gcctcctcca
     1561 ccgacacggg gcgggccacc cgggccaccg gcacgccgtc gacggccgtg cccggcgggg
     1621 ccggctggac caggtcgccg cgcgccagct cctggaggcc cacggctcag aacacctccg
     1681 ccaggccggc ctgctcggcc gggtggaccc ggcgcgggcc gggacgctcg ccgcacagcc
     1741 gcggggtggg gaacaccttg cccgggttgg cccgtccctc ggggtcgaag gcgcagcgca
     1801 ccagctgcat ggtgtccagg tcctcgggcg tgaactgccg gcccatgtac tgcttcttgt
     1861 cgaagccgac gccgtgctcg ccggtgatcg acccgccgtg ctccaggcag gtgtagagga
     1921 tctcggcggc cagctcctcg gcccgctgct cctggccgga gacctcgccg tcgtacagca
     1981 ccagcgggtg caggttgccg tcgccggcgt ggaacacgtt ggccacccgc agcccgcgct
     2041 cgtcggccag ctcggtgatc cgccgcagca cctcgggcag ggcggtccgg gggatgaccc
     2101 cgtcctggac gtagtagtcg gggctgatcc gcccaacggc ggcaaaggcc gacttgcggc
     2161 ccttccagaa cagggcccgc tggtcgtcgt cctcggcgat ctggatggtg gtcgagccgg
     2221 cctcctggca gtgccgctcg acctcgtcga actggtgctc gacctcggcc gccggcccgt
     2281 ccagctccac caccaggacc gcgccagccc cttccgggta gtgcacgtgc acggccgcct
     2341 cggcggcctc catggccagg gcgtccatca tctcgatggc cgccggcacg atcccggccc
     2401 cgatgatcct ggacacggcg tcgccggcct ggtcggtgcc cttgaacgcg gccagcaggg
     2461 tgcggacggc ctcgggcttg cgcagcagcc gcagggtcac cttggtggtg atgccgaggg
     2521 tgccctcgga gccgacgaag gcgccgagca ggtcgtagcc cggcgggtcg ggcgccttgc
     2581 cgccccccag gtggacgagc tcgccgtcgg gcagcaccac ctcgaggccg gtgacgtggt
     2641 tggtggtgaa gccgtacttg aggca
//
LOCUS       DASSWR010000042         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_538550,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000042 DASSWR010000000
VERSION     DASSWR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538550"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1219
                     /locus_tag="VFO47_00750"
     CDS             185..1219
                     /locus_tag="VFO47_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET8787258.1"
                     /translation="MAIVGGLDIHRRQITYDYLDTDSGETCRGQLSPATRLELRAWLA
                     RFTGQPAAFALEATTGWRFVAEELARAGIRAHLAEPADTRALRGPKRRAKTDRTDARH
                     LRELLRAGTLPESWMPPAHIADARTQVRLRRALVGEHTGWYQRIHAVLFHHGLPERSQ
                     LLTADGQAWLARAELPAVARQTVDLALRMIDHLDTELDLLDTELARIARAQPGCKALM
                     GRYGIGWLTAVAIWAEFGDVRRFTGSRQAVRFTGLDITISESDGKRARGHLARQGSPV
                     LRWALHEAAMCAARPGSPDHAYYLQVKQRLGGKRAALSVARKLAREAYHTLRELGDQA
                     LVPAELPTAA"
     gene            1891..2886
                     /locus_tag="VFO47_00755"
     CDS             1891..2886
                     /locus_tag="VFO47_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8787259.1"
                     /translation="MTERTTRAEDGAQGRGGRGGQAPQRPTQLPGRSWWAAVKRTVRE
                     FQVDNLSDWAAALTYYSVLSIFPALLVVISLVDLAGPGTIQTLLDNLGQVVPGSVNQI
                     LATAIDNLQQTRGSAGLLALVSLATALWLASRYVAAFMRASNAIYDVPEGRPVWKTLP
                     IRIGVTVVVMVLLAVSAVAVVATGGLAARIGRLLGIGSTAVRVWDIAKWPVLLLIVGF
                     LFALLYWASPNARQPFRWITPGGILAVVVWVLASVAFALYVANFNSYNKTYGSLASVI
                     IFLVWLWLSNTAILLGAELNAELERGRAIAAGHPPEEEPYMELRDTSKLRERDNL"
BASE COUNT          450 a         1105 c         1015 g          481 t
ORIGIN      
        1 gagggctacc gggccatgga cgagcgccgc gccatcaaga ccctgctacg accgtagatg
       61 ccgactctcg cagcagtccc cagcggttga cgcgaaatag atggcatgtg agcgggaccc
      121 cctccgctcg gtgcggcacg ctggccgcat cggatcgcaa acgcgggagc cagaggaggt
      181 cccgatggcc atcgtaggag ggctcgacat ccaccgccgc cagatcacct acgactacct
      241 ggataccgac agcggcgaga cctgtcgtgg gcagctcagc ccggccactc gcctggagct
      301 gcgcgcctgg cttgctcggt tcactggcca gccggcggcc ttcgccttgg aggccaccac
      361 cggctggcgg ttcgtcgccg aggagctggc gcgggccggg atccgagcgc acctggccga
      421 gccggccgac acccgggcgc tgcgcggtcc caagcggcgg gccaagaccg accgcaccga
      481 cgcccgccac ctgcgggagc tgctgcgcgc cggcacgctg ccagagtcgt ggatgccacc
      541 ggcgcacatc gctgacgcac gcactcaggt ccggctgcgc cgtgcgctgg tcggcgagca
      601 caccggctgg taccagcgca tccacgcggt gctgttccac catgggctgc ctgagcgctc
      661 ccagctgctc accgccgacg gacaggcctg gctggcccgg gccgagctcc ctgcggtggc
      721 ccgccagacg gtcgacctgg ccctgcggat gatcgaccac ctcgacaccg aactcgacct
      781 gctcgacacc gagttggcgc gcatcgcacg agcccagcca ggctgcaagg cgctcatggg
      841 tcgctatggg atcgggtggc tgaccgcggt ggcgatctgg gccgagttcg gcgacgtgcg
      901 ccgcttcaca ggctcccgcc aggcggtccg cttcactggc ctggacatca ccatcagcga
      961 gtccgacggc aagcgcgccc gcgggcacct ggcccgccag ggctccccgg tgctgcgctg
     1021 ggcgttgcac gaggcggcca tgtgcgctgc ccgcccgggc tcccccgacc acgcctacta
     1081 cctgcaggtc aagcagcggc tgggcggcaa gcgggctgcg ctgtcggtgg cgcgcaagct
     1141 ggcccgcgag gcctaccaca ctctgcgcga gctcggcgac caggcactgg tccccgccga
     1201 actcccaacc gccgcctgag ctggctggca tcgtgcgcgc ttgggcgcct ggattgttga
     1261 tgtggtgcgg ctggctccct gatgctcctg ccgccacgct cgtcgtgggc ggcctccata
     1321 gaacgagcgg ccgcgcccgc cacgtgcggg aacccgatca cccatcatgt cgccggaccc
     1381 tggtcggtcc gagcaccgag ataaggctgg atgcccgcgc gcggatccct gccagcagac
     1441 caagcctcgc ccggtgatgc ccatggcatg acgcaggctg ctgcttcgct ccacgatcct
     1501 gcggcacccc tcgccggcgt caaggtcggt cgtcctcgcg ctgctgcggg cgctccacct
     1561 tgacccacgg cttcggtctg cccgacccag ccagcaacaa aggagcagca gacccgacca
     1621 aatcctcgtt gaccggggat cccaccacag ataagaagcg tcaagcggaa tgaccagcgc
     1681 gcctgggccg ctggtccagg tcgccacgca gatcaatctg ctggcagccc agtcgacggt
     1741 ggtgagggtc agttaggccc gaggtggtcg gggtactgct cacctgtggc agcaaccgag
     1801 ccggttggct tcagtgcggt ttcgcgaccc cggcgggatc ggacagccgc aacccgctcg
     1861 gcccagccgc gctgaggagg tgctggtgcc atgacggagc gcaccaccag ggctgaggac
     1921 ggggcccaag gtcgtggagg ccggggcggc caggcccccc agcggccgac ccagctgccc
     1981 ggtcgttcct ggtgggcggc ggtcaaacgg accgtgcggg agttccaggt cgacaacctg
     2041 agcgactggg cggcggcgct cacctactac agcgtgctgt cgatcttccc ggccctgctg
     2101 gtggtgatct ccctagtcga tctggccggc cctgggacga tccagaccct gttggacaac
     2161 ctcggccagg tcgtgcccgg ctcggtcaac cagatcctgg ccaccgccat cgacaacctg
     2221 cagcagaccc gcggctcggc tgggctgctg gccctggtca gcttggccac ggccttgtgg
     2281 ctggcctccc ggtacgtcgc cgcctttatg cgcgcctcca acgccatcta cgacgtgccc
     2341 gagggtcggc cggtctggaa gaccctgccc atccgcatcg gggtcaccgt ggtcgtcatg
     2401 gtcctgctgg cggtgtcggc ggtcgcggtc gtggccaccg gcggcctggc tgctcgtatc
     2461 ggccgactgc tggggattgg ctccaccgcg gtcagggtct gggacatcgc caagtggccg
     2521 gtgctgctgc tgattgtcgg ctttctgttc gccctgctgt attgggcgtc ccccaacgcc
     2581 aggcagccgt ttcgctggat cacccctggc gggatcctcg ccgtcgtggt ctgggtgctc
     2641 gcctcggtcg ccttcgcgct gtatgtggcc aacttcaact cctacaacaa gacctacggc
     2701 agcctggcca gcgtgatcat cttcctggtc tggctgtggc tgtccaacac cgccatcctg
     2761 ctcggggcgg agctgaatgc cgagctggaa cgcggccggg ccatcgccgc cggtcatcca
     2821 cctgaggagg agccctacat ggagctgcgc gacaccagca agctccggga acgcgacaac
     2881 ctgtagccca cagccacacg ccccggctcc ccgaactcca tatgcaccag atccgtagcc
     2941 tggtgctccg cgtcgatctg gtcggctcca gaccgatctg gcctgctcac gttgggtgcc
     3001 tcgtccgtcc agacggatcc agacggatcc agacggatcg tctggatgat c
//
LOCUS       DASSWR010000043         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_255942,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000043 DASSWR010000000
VERSION     DASSWR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255942"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1839)
                     /locus_tag="VFO47_00760"
     CDS             complement(139..1839)
                     /locus_tag="VFO47_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET8787260.1"
                     /translation="MDQPGGAPAGGHVVLCGLNELGYRTLEELVRLGVEVVVVVRSPA
                     EELARGARELGATLVPGNYRDQAVLAAAGVAAARALVVTEDDDVGNLHAALAAQELNP
                     ALRMRLRMFNRELGRRVEALFEDCQVFDSAALAVPAFVSAALLQDWQQRVEVAGRTLV
                     VRRAAATDPGVLLPLARVHPDGAAELFPDGGQELLCLSWAPGNRRTRRPERPPVEHPG
                     RVAAAWTVLLGADIRLRVMTAIVLGLTVTGIAIFWWFSEQDLDLIDAIYFTVTIMTTT
                     GFGDIHLRNAPPPLQLYGVALMLSGTAALAILFALITDALISARLARVLGANIPRGLH
                     DHVIVCGLGNIGYRMVEQLHELGVPLVAAELHESNRYLPAVRRLGVPVLVADIRLPET
                     LQTLHVERARSVVVVTSSDIVNLETALNTQTLRPEVRVVLRLFDPDLAARVERAFGIH
                     ISRSPSALAAPAFAAAAAGEHVLATIAVGAAVLGVVRLRVAPGCRVQGHTVAELEAAS
                     NSRVLLLDDGAGTTWRPRGTTRLDAGVELVVVIPRGELGRVLAWTETDVHATPDPSPS
                     "
     gene            complement(1849..2052)
                     /locus_tag="VFO47_00765"
     CDS             complement(1849..2052)
                     /locus_tag="VFO47_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787261.1"
                     /translation="MAEELPPGVIDNDGHYEVDPALMSVYPQFATDFEVQRIVTDRLE
                     YLYWVQRHFADQVLPPHAEGPSG"
     gene            complement(2055..>3048)
                     /locus_tag="VFO47_00770"
     CDS             complement(2055..>3048)
                     /locus_tag="VFO47_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8787262.1"
                     /translation="VISLEVVLPTGELLEVGQGGGRKIRHSSTGYRLKDLFLGHQGTL
                     GIATQATLELYPRPEAEFPAFFGYESFEAGYQAMQPFCRSNLATLAGVILFDERKVQY
                     LRRDDEAYIPEPESLQSIVAVAAYGSEGEVAAARDRLMRIGADTGGRYLGEELSELDW
                     ASRHDRYATPLHGRSRDRAVHMSWHCEDAAITYSQLPFVKQRWHEIADRYIAEHEVFD
                     NWGMFFYTNNAYKGWGDFLVEIDIGIWEQRLDEASWRAWTDMKREITQVSLDAGGSIS
                     ACHGATREGEVELVPAELGGQFDLMLKLKRMLDPNNIMNPGKYLLDQAYEDGEG"
BASE COUNT          423 a         1073 c         1151 g          401 t
ORIGIN      
        1 gctgcgggcc gagcggccgg gtgcgccggc cgacccccag gtcgaccggg caggcgccgg
       61 tgaccagccc gacgtacccg gccgccgcca cggcggcgcc gccggcggcg tagccgagca
      121 gccgagggat cgaggtggtc atgaggggct ggggtcgggg gtggcgtgga cgtcggtctc
      181 ggtccaggcg aggacgcggc ccagctcgcc gcgggggatg accacgacca gctcgacgcc
      241 ggcgtccagc cgggtcgtcc cccgaggccg ccaggtggtg ccggcgccgt cgtccagcag
      301 caggacccgg ctgttggagg cggcctccag ctcggccacc gtgtggccct ggacccggca
      361 gccgggggcc acccgcagcc ggacaacgcc cagcaccgcc gccccgacgg cgatcgtggc
      421 caggacgtgc tcgccggcgg cggcggccgc gaacgcgggg gcggccaggg ccgaggggct
      481 gcgggagatg tggatgccga aggcccgctc gacccgggcg gccagatcgg ggtcgaacag
      541 ccgcagcacc acccggacct cgggacggag ggtctgggtg ttcagggccg tctcgagatt
      601 gacgatgtcg ctggaggtga ccaccaccac cgagcgggcc cgctcgacgt gcagggtctg
      661 gagcgtctcg ggcaggcgga tgtcggccac cagcaccggg acgccgaggc ggcgcaccgc
      721 cggcaggtag cggttgctct cgtgcagctc ggcggcgacc agcggcacgc ccagctcgtg
      781 gagctgctcc accatccggt agccgatgtt gccgagcccg cagacgatca cgtggtcgtg
      841 cagcccgcgg gggatgttgg cgcccaggac gcgggccagc cgggcgctga tcagcgcgtc
      901 ggtgatcagc gcgaacagga tggccagggc ggccgtgccc gacagcatca gggcgacgcc
      961 gtagagctgc agcggcggcg gggcgttgcg caggtggatg tcgccgaagc cggtggtggt
     1021 catgatcgtg accgtgaagt agatggcgtc gatcaggtcg aggtcctgct ccgagaacca
     1081 ccagaagatg gcgatgccgg tcacggtcag cccgaggacg atggccgtca tgacccgcag
     1141 gcggatgtcg gccccgagca gcaccgtcca ggcggcggcg acccggccgg ggtgctccac
     1201 cggcggccgc tccgggcgcc tggtccggcg gttgccgggc gcccaggaca ggcagagcag
     1261 ctcctggccg ccgtcgggga acagctcggc cgcgccgtcg gggtggaccc tggccaacgg
     1321 cagcagcacg ccgggatcgg tggcggcggc ccgccgcacg accagggtcc ggccggccac
     1381 ctcgacccgc tgctgccagt cctggagcag cgccgccgac acgaacgcgg gcacggccag
     1441 ggcggccgag tcgaacacct ggcagtcctc gaacagcgcc tcgacccggc ggcccagctc
     1501 gcggttgaac atccgcagcc gcatccgcag ggccgggttg agctcctggg cggccagggc
     1561 ggcgtgcagg ttgccgacgt cgtcgtcctc ggtgacgacc agggcccggg ccgccgccac
     1621 cccggccgcg gccagcaccg cctggtcgcg gtagttgccc ggcacgaggg tggcgccgag
     1681 ctccctggcc cccctggcca gctcctcggc cggcgaccgc acgaccacga cgacctcgac
     1741 cccgaggcgc accagctcct cgagcgtccg gtagccgagc tcgttgaggc cgcacagcac
     1801 cacgtgcccg ccggccgggg cccctcccgg ttggtccatc gccgattgct acccgctggg
     1861 accctcggca tgcggcggga gcacctggtc ggcgaagtgg cgctgcaccc agtacaggta
     1921 ctcgaggcgg tcggtgacga tccgctggac ctcgaagtcg gtggcgaact gggggtagac
     1981 gctcatcagg gccgggtcga cctcgtagtg gccgtcgttg tcgatcacgc ccggcggcag
     2041 ctcctcggcc atgctcagcc ctccccgtcc tcgtaggcct ggtcgagcag gtacttgccc
     2101 gggttcatga tgttgttggg gtcgagcatc cgcttgagct tcagcatcag gtcgaactgg
     2161 ccgccgagct cggccgggac cagctcgacc tcgccctcgc gggtggcgcc gtggcaggcc
     2221 gagatcgacc cgccggcgtc cagcgacacc tgggtgatct cccgcttcat gtcggtccag
     2281 gcccgccagc tggcctcgtc caggcgctgc tcccagatgc cgatgtcgat ctcgaccagg
     2341 aagtcgcccc agcccttgta ggcgttgttg gtgtagaaga acatccccca gttgtcgaac
     2401 acctcgtgct cggcgatgta gcggtcggcg atctcgtgcc agcgttgctt gacgaacggc
     2461 agctgggagt aggtgatggc cgcgtcctcg cagtgccagc tcatgtggac ggcccggtcg
     2521 cgggagcggc cgtgcagcgg ggtcgcgtag cggtcgtggc gcgacgccca gtccagctcc
     2581 gacagctcct cacccaggta gcggccgccg gtgtcggcgc cgatgcgcat cagccggtcg
     2641 cgggcggccg cgacctcgcc ctcggagccg taggcggcca cggccacgat cgactggagc
     2701 gactccggct cggggatgta ggcctcgtcg tcgcgccgga ggtactggac cttgcgctcg
     2761 tcgaacagga tcaccccggc cagggtggcc aggttggagc ggcagaacgg ctgcatggcc
     2821 tggtagccgg cctcgaacga ctcatagccg aagaaggccg ggaactcggc ctcgggccgc
     2881 gggtacagct ccagggtggc ctgggtggcg atccccaggg tgccctggtg gccgaggaac
     2941 aggtccttga gccggtagcc ggtggagctg tggcggatct tgcggccgcc gccctggccg
     3001 acctcgagca gctcgccggt gggcagcacc acctccaggc tgatcacg
//
LOCUS       DASSWR010000044         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_171316,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000044 DASSWR010000000
VERSION     DASSWR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171316"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFO47_00775"
     CDS             <1..472
                     /locus_tag="VFO47_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET8787263.1"
                     /translation="YGQLDLHLRQDPRVLVLDRTNVRTLEADQVGGPVDLTVADLSFI
                     SLRLVVPVLARLTRDDGDLVLLVKPQFEAGREAVGRGGVVRDPAARAAAVRAVAGAGA
                     SLGLGVAGLCPSPLPGPAGNLELFLHLRAGAPPLDEPALEAALAEGAALPGAAG"
     gene            469..1365
                     /locus_tag="VFO47_00780"
     CDS             469..1365
                     /locus_tag="VFO47_00780"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET8787264.1"
                     /translation="MSRLRRVGVVVHPGRPPAVELGKLLAGWADEHGIELRTLASERG
                     CVGEDVPCVEEPEFAPGLDLVVALGGDGTLLRALGLVLVEQVPALGVNAGRLGFLVEV
                     EPGGLGDALDAVWAGRYRVERRTTLVARVVVDGREIAEDLAVNDVVLEKAARERLAAV
                     AVRLGDGGPFARYAADGVMVASPTGSTAYSFSAGGPIVSPRLDALLLTPIAPHMVFNR
                     SLVLHPDEAVQLEVLPDNPVIESVDGRAARELPPGAVVEVRRSRFDALLVRIGRADFY
                     RLVRSKFRLADPAATDAKHAGP"
     gene            1419..>2377
                     /locus_tag="VFO47_00785"
     CDS             1419..>2377
                     /locus_tag="VFO47_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787265.1"
                     /translation="MLAELRIRDLGVIDDARLEVSAGLNVLTGETGAGKTMVVDALAL
                     LLGGRADPGAVRAGRPAALVEGRLRTGGDGEVAAALATAGVEDEDGEVVVARQVLAEG
                     RSRAQLQGRMATVAAVADVVRPLVEVHGQHEFQDLLRPGVQRDLLDRFAGEPALGPRA
                     AFAAGWRRLRAVTRELDDLATRAREREREIDLLRHQLDEIEAAQVRVGESAELAAEAE
                     RLANAEALQQAAALAHQLLEGDEDAGAATALGAAARAVQGPGGHDRALGELAGRAQAL
                     AAEVGDLASSLRAYAEAVLVDPARLEEVNARMAQLRDLERKYG"
BASE COUNT          246 a          933 c          911 g          287 t
ORIGIN      
        1 ctacggacag ctcgacctgc acctgcgcca ggacccgagg gtcctggtgc tggaccggac
       61 caacgtccgc accctggagg ccgatcaggt cggcgggccg gtcgacctga ccgtggccga
      121 cctgtcgttc atctcgctgc gcctggtcgt gcccgtcctg gcccggctga cccgggacga
      181 cggcgacctg gtgctgctgg tcaagcccca gttcgaggcc ggccgggagg cggtgggccg
      241 cggcggcgtg gtccgcgacc cggccgcccg ggccgccgcc gtccgcgcgg ttgccggcgc
      301 cggcgcctcc ctcgggctcg gggtggccgg gctctgcccg tcgccgctcc ccggcccggc
      361 cggcaacctg gagctgttcc tccacctccg cgccggcgca ccccccctcg acgagcccgc
      421 cctcgaggcc gccctggccg agggcgccgc cctccccgga gccgcggggt gagccgcctg
      481 cggcgggtcg gggtggtggt ccacccaggg cggccgcccg ccgtcgagct gggcaagctg
      541 ctggccgggt gggccgacga gcatgggatc gagctgcgca ccctggcctc cgagcggggc
      601 tgtgtcggcg aggacgtgcc ctgtgtcgag gagccggagt tcgcccccgg gctcgacctg
      661 gtggtcgcgc tcggcggcga cgggaccctg ctgcgggccc tcggcctggt cctggtcgag
      721 caggtgccgg cgctgggggt gaacgccggc cggctcgggt tcctggtcga ggtggagccg
      781 ggcggcctcg gcgacgccct cgacgccgtc tgggccggcc gttaccgggt cgagcggcgc
      841 accaccctgg tggccagggt ggtcgtcgac ggccgggaga tcgccgagga cctggccgtc
      901 aacgacgtcg tgctcgagaa ggcggcccgg gagcggctgg ccgcggtcgc ggtccgcctc
      961 ggcgacggcg ggccgttcgc ccgctacgcc gccgacgggg tcatggtcgc ctccccgacc
     1021 ggctcgaccg cctacagctt ctcggccggc gggccgatcg tctccccccg gctggacgcg
     1081 ctgctgctca ccccgatcgc gccccacatg gtcttcaacc gctcactggt cctgcacccc
     1141 gacgaggcgg tgcagctcga ggtgctcccg gacaacccgg tgatcgagag cgtcgacggc
     1201 cgggccgccc gcgagctgcc cccgggcgcg gtcgtggagg tccgccgcag ccgcttcgac
     1261 gccctgctcg tccggatcgg gcgggccgac ttctaccgcc tggtccgctc caagttccgc
     1321 ctggccgacc ccgccgccac cgacgccaag cacgccgggc cctgagctgg ggacggcgcc
     1381 gggctccggg tgggccaggc gggctagaat cgcaggtgat gttagccgag cttcgcatcc
     1441 gcgacctcgg ggtcatcgat gatgcccgcc tcgaggtctc ggccggcctc aacgtgctca
     1501 ccggcgagac cggcgccggc aagaccatgg tcgtcgacgc cctcgccctg ctgctcgggg
     1561 ggcgggccga ccccggcgcg gtccgggccg gtcggcccgc cgcgctggtc gaaggccgcc
     1621 tgcgcaccgg cggcgacggc gaggtcgccg ccgccctggc gacggccggg gtggaggacg
     1681 aggacggcga ggtcgtggtc gcccgccagg tgctggccga gggccgcagc cgggcccagc
     1741 tccagggccg catggccacc gtggccgccg tggccgacgt cgtccgcccc ctggtcgagg
     1801 tccacggcca gcacgagttc caggacctgc tccgcccggg cgtccagcgc gacctgctcg
     1861 accgcttcgc cggcgagccc gcgctgggcc ctcgggccgc cttcgccgcc ggctggcggc
     1921 ggctgcgcgc cgtcacccgc gagctggacg acctggccac cagggcccgc gagcgcgaac
     1981 gcgagatcga cctgctccgc catcagctgg acgagatcga ggccgcccag gtccgggtgg
     2041 gggagtcggc cgagctggcc gccgaggccg agcgcctggc caacgccgag gccctccagc
     2101 aggcggccgc cctggcccac cagctcctcg agggcgacga ggacgccggc gcggccaccg
     2161 ccctcggcgc ggcggccagg gcggtgcagg gccccggcgg ccatgaccgc gccctcggcg
     2221 agctggccgg gcgggcccag gcgctggccg ccgaggtcgg cgacctggcc tccagcctgc
     2281 gcgcctacgc cgaggcggtg ctggtcgacc cggcccgcct cgaggaggtc aacgcccgca
     2341 tggcccagct ccgcgacctg gagcgcaagt acggcga
//
LOCUS       DASSWR010000045         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_199541,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000045 DASSWR010000000
VERSION     DASSWR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199541"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..>2073)
                     /locus_tag="VFO47_00790"
     CDS             complement(43..>2073)
                     /locus_tag="VFO47_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET8787266.1"
                     /translation="RAERITRLIASGQPAPDRFQGAWARLRAELVEVFGQRKLLKRPL
                     PGLAHFFTFWGFVILLTTIAEAYGELFSETFALPLVGHSPLLGFLQDLTAVAVLVSLA
                     VFAVIRIRQSPARQDRASRFYGSHTGQAWVILGMIALVIVTLLLYRGARVARGTFPYD
                     GWAFASTAVGRALDGLAPSTLRLLDEGFLLAHLAVVLGFLVLIMYSKHLHIVTAPFNV
                     AFSRRPKALGPLATPRIDPEEMTEEDVFGAGKVEDLNFKQLLDMATCTECGRCQDQCP
                     AWNTGKPLSPKMVITDLRDHLFEKAPFLLGEADPEHPDGHDPAVVGKDLVQDVVGADV
                     LWSCTTCGACVEQCPVDIEHVDTIMELRRYEVLMESRFPAEAGTMLRNVENSGDPWGL
                     GAGQRLAWTEGLGFEVPVVTDRIPDDVEYLYWVGCAAALEDRARKTARAVAGLLHRAG
                     VRFAVLGPRESCSGDPARRLGNEYLYQLQAQQNIETLDGVGARKVIASCPHCFNTLAR
                     EYPHLGGNYEVIHHTQLLRRLVDEGRLRPDGPVETTVTYHDPCYLGRYNDVYDAPRAV
                     LDAVPGVRSVEMHRCRERGFCCGAGGARMWMEERIGKRVNLERTDEALATGAEVVGTA
                     CPYCMVMLDDAIKQRQAEGAAEGVRVLDVAQVLEESVRVRERPAADAPSDAP"
BASE COUNT          296 a          713 c          794 g          270 t
ORIGIN      
        1 gcgcgacccg gtggtccagc ccagggacac cggcgagagc ccctagggag cgtctgaggg
       61 ggcgtcggcg gccgggcgct cccggacccg gaccgactcc tccagcacct gggcgacgtc
      121 gaggacccgg accccctcgg cggcgccctc ggcctggcgc tgcttgatgg cgtcgtcgag
      181 catgaccatg cagtaggggc aggccgtccc gaccacctcg gccccggtgg ccagggcctc
      241 gtcggtgcgc tccaggttga cccgcttgcc gatgcgctcc tccatccaca tgcgggcccc
      301 gccggcgccg cagcagaagc cccgctcgcg gcagcggtgc atctccaccg accggacccc
      361 gggcacggcg tcgagcacgg cccgcggggc gtcgtagacg tcgttgtagc ggcccaggta
      421 gcaggggtcg tggtaggtga cggtggtctc gaccggcccg tcggggcgaa ggcggccctc
      481 gtcgaccagc cgccgcagca gctgggtgtg gtggatgacc tcgtagttgc cgcccaggtg
      541 ggggtactcg cgggccaggg tgttgaagca gtgggggcag gaggcgatca ccttgcgagc
      601 cccgaccccg tccagggtct cgatgttctg ctgggcctgg agctggtaga ggtactcgtt
      661 gccgagccgc cgggccgggt cgccggagca ggactcgcgc gggccgagca cggcgaagcg
      721 caccccggcc cggtgcagca ggccggcgac ggccctggcc gtcttgcggg cccggtcctc
      781 gagcgcggcc gcgcagccga cccagtagag gtactcgacg tcgtcgggga tccggtcggt
      841 gaccaccggg acctcgaacc cgagcccctc ggtccaggcc aggcgctggc cggcccccag
      901 cccccagggg tcgccggagt tctcgacgtt gcgcagcatg gtgccggcct cggcggggaa
      961 gcgggactcc atcaggacct cgtagcggcg cagctccatg atggtgtcga cgtgctcgat
     1021 gtcgaccggg cactgctcga cgcaggcccc gcaggtggtg caggaccaga gcacgtccgc
     1081 cccgaccacg tcctggacga ggtccttgcc gaccaccgcc gggtcgtggc cgtccgggtg
     1141 ctcggggtcg gcctcgccga gcaggaacgg ggccttctcg aacaggtggt cgcgcaggtc
     1201 ggtgatgacc atcttgggcg acagcggctt gcccgtgttc caggccgggc actggtcctg
     1261 gcagcggccg cactcggtgc aggtggccat gtcgagcagc tgcttgaagt tcaggtcctc
     1321 gaccttgccg gcgccgaaga cgtcctcctc ggtcatctcc tcggggtcga tccggggggt
     1381 ggccagcggc cccagggcct tggggcgccg cgagaaggcg acgttgaacg gggcggtgac
     1441 gatgtgcagg tgcttggagt acatgatcag caccaggaag ccgagcacga ccgccaggtg
     1501 ggccagcagg aagccctcgt cgagcagccg cagggtcgac ggggcgaggc cgtcgagggc
     1561 ccgcccgacg gccgtggacg cgaacgccca gccgtcgtac gggaaggtcc cgcgggccac
     1621 cctggccccc cggtacagca gcagggtgac gatcaccagg gcgatcatgc cgaggatgac
     1681 ccaggcctgg ccggtgtggg acccgtagaa ccgcgaggcc cggtcctggc gggcgggcga
     1741 ctggcggatg cggatgacgg cgaagacggc caggctgacc agcacggcga ccgcggtcag
     1801 gtcctggagg aacccgagca gggggctgtg ccccaccagc ggcagggcga aggtctcgct
     1861 gaacagctcc ccgtaggcct cggcgatcgt ggtcagcagg atcacgaagc cccagaaggt
     1921 gaagaagtgg gccagcccgg ggagcggccg cttgagcagc ttgcgctggc cgaagacctc
     1981 gaccagctcg gcccggaggc gggcccaggc gccctggaac cggtcggggg ccggctggcc
     2041 gctggcgatc agcctggtga tccgctccgc ccg
//
LOCUS       DASSWR010000046         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_171371,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000046 DASSWR010000000
VERSION     DASSWR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171371"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3130)
                     /locus_tag="VFO47_00795"
     CDS             complement(<1..3130)
                     /locus_tag="VFO47_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8787267.1"
                     /translation="MQFGILGPLEVRNGEAPVRVPGAKERALLADLLVNAGRVVSADR
                     LVEDLWGDDPPGRPANTLQGRVSALRRALGPGGAGRLVTSPPGYRLDAGLEQLDATRF
                     ERLVAEAEAAAPAEPPRAVRLLEEALGLWRGPALAEFADQPWAQAEAARLEELRLAAR
                     EALVELRLAAGGHAGLVGELEALVAAHPTRERLRGQLMVALYRSGRQADALAVYQQTR
                     EVLAEELGIDPSPELQRLHQAILVQDPALEAAARDRDLPRHNLPERLTSLVGRDQELA
                     ELAKLLEQHRLVTVAGPGGAGKTTVAVELARRLMGGYPDGVWLVELAPLRDPALLAEV
                     VVAALGLGEEAADRAAPPPSPAERLAGFARDKALLLVLDNCEHLVGACAALVQGLLRS
                     GPAVRVLATSREVLGVPGEVVWPVPPLAVPAAGDAAAGLAGPDAGGAGPEVLAGYDAV
                     RLFGERAAAADPGFTLDAASAPVVAELCRRLDGLPLAIELAASRVRALPVAEIADRLE
                     DRFRLLAGGGRTLDPRQQTLRATVDWSWDLLEEPDRRLWRRLSVFSGGWTVAAAEEVC
                     GGDGLEAGDVLEGLFRLVDRSLVVAAGGDPPRFRMLETMRVYGAERLAEAGEHETMAA
                     RHTAWCLALAERAAAHRTARRWLRLLDADYDNLRAALDRTMAAGDPGTALRLAADLGW
                     YWWTRHTPEGHQRLAAVIALADADGRPPTPDLARALQASAMLAVAMTPTAAATDAVQR
                     SQELFERYGDRWGVAFSQLLSAWVGLQRFGPNADAIRLVEQAEATFRELGDPWGEAFA
                     GQSRFALEAYLHGLSERGEAAGRRALERFQALDDQWGLAQAQVTRAELASARGDVDGA
                     AAAYEAALVAAGDVGPGWVQLASIVRLGTMVALQGDDARAAVLGVEAADRIRRFGERR
                     GFAHLYNEMGGVARARDDLERARHLHREALPIVQELVGWSVPYTLASLACAEARLGDL
                     DEAEAHLQEAAALLVAAPQATTVALVLTGAALAAIGRGRPELAARRLAAAEAARARAG
                     VVPIGGEAREADL"
     gene            3221..3613
                     /locus_tag="VFO47_00800"
     CDS             3221..3613
                     /locus_tag="VFO47_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8787268.1"
                     /translation="MQEREVRELFERLRTAMDPAIEYEERHEDYLVEFPQSGERLDRD
                     GLRRLQETFPGGTPPRIRLRRLTGGGDVWFAESAIHYADGKDFFGVSRVEFRDGKMWR
                     ETRYYAEPFEVPEWRAEWSNPVLEEAAR"
     gene            complement(3615..4595)
                     /locus_tag="VFO47_00805"
     CDS             complement(3615..4595)
                     /locus_tag="VFO47_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8787269.1"
                     /translation="MRVMVLGGTRFVGAAVVEELAAHGHELLVVHRGEHEPAGLPDAD
                     HLHTDRQDLPHLRGPVDEFDPEAVVDVCAYSAADAETALAAVGGDVRLLVVSSMDVYR
                     AFGAVLAGTETDALPVDETSPVRSERYPYRGRTLTSTDADTYEKLDVEAAYLARAATV
                     CRLPMVYGERDHQRREEPLLRRVRAGRDRIPAGSGGWLWTRGYVRDVAAGIRLALESD
                     ACVAEILNLGEARTWSMGLWARHVLEAAGSEAELVRVPDVLLPDDLKALGTVSQHLLV
                     DSSKARDLLGWTETDPHEALHRSVAWHLANPPEGADADFTADDRALAAAG"
BASE COUNT          575 a         1837 c         1756 g          503 t
ORIGIN      
        1 ccagatcggc ctcgcgggcc tccccgccga tcgggaccac gccggcgcgc gcgcgggccg
       61 cctcggcggc ggccagccgg cgcgcggcca gctcgggccg gccccggccg atcgcggcca
      121 gggccgcgcc ggtcaggacc agggccacgg tcgtcgcctg gggcgcggcg accaggaggg
      181 cggccgcctc ctgcaggtgc gcctcggcct cgtcgaggtc gccgaggcgg gcctcggcgc
      241 aggcgagcga ggcgagggtg taggggacgc tccagccgac cagctcctgg acgatgggca
      301 gcgcctcccg gtgcaggtgg cgagcgcgtt ccaggtcgtc gcgggcccgt gcgacgccgc
      361 ccatctcgtt gtacaggtgg gcgaacccgc gccgctcccc gaaccggcgg atccggtcgg
      421 ccgcctcgac gcccaggacg gccgcccggg catcgtcgcc ctggagggcg accatggtgc
      481 cgagccgcac gatcgaggcc agctgcaccc accccggccc cacgtcgccg gcggcgacca
      541 gggcggcctc gtaggcggcc gccgccccgt cgacgtcgcc ccgggcgctg gccagctccg
      601 ccctggtcac ctgcgcctgg gccagccccc actggtcatc gagggcctgg aaccgctcca
      661 gggcccgccg gcccgccgcc tcgccccgct ccgagagccc gtggaggtag gcctccaggg
      721 cgaaccggga ctggcccgcg aaggcctcgc cccaggggtc gcccagctcc ctgaaggtcg
      781 cctcggcctg ctcgaccaga cggatggcgt cggcgttggg gccgaaccgc tgcagcccca
      841 cccaggccga cagcagctgg gagaaggcga ccccccagcg gtccccgtac cgctcgaaca
      901 gctcctggct gcgctggacg gcgtcggtgg cggccgcggt cggggtcatc gccaccgcca
      961 gcatggcgct cgcctgcagc gccctggcca ggtccggggt cggcggccgc ccgtcggcgt
     1021 cggccagggc gatgaccgcg gccagccgct gatggccctc cggggtgtgc ctggtccacc
     1081 agtaccagcc gaggtcggcg gccagccgca gcgccgtgcc ggggtcgccg gccgccatcg
     1141 tccggtccag ggccgcccgc aggttgtcgt agtcggcgtc gagcagccgc aaccagcgcc
     1201 gggcggtgcg gtgggcggcg gcccgctcgg ccagggccag gcaccaggcg gtgtggcggg
     1261 cggccatcgt ctcgtgctcg cctgcctcgg ccagccgctc ggccccgtag acccgcatcg
     1321 tctccagcat ccggaagcgc ggcgggtcgc cgccggccgc caccaccagc gaccggtcca
     1381 ccagccggaa cagcccttcc agcacgtccc cggcctccag gccgtcgccg ccgcagacct
     1441 cctcggcggc cgccaccgtc cagccacccg agaacaccga cagccgccgc cacagccgcc
     1501 ggtcgggctc ctccagcagg tcccagctcc aatcgacggt ggcccgcagg gtctgctgcc
     1561 gcgggtcgag ggtgcggccg cccccggcca gcagccggaa ccgatcctcc agtcggtccg
     1621 cgatctcggc caccggcagg gcccgcaccc tggacgcggc cagctcgatg gccaggggga
     1681 ggccgtccag gcgacggcac aactcggcca ccaccggggc gctggccgcg tccagggtga
     1741 agcccgggtc ggcggcggcc gcccgctccc cgaacagccg caccgcgtcg tagccggcca
     1801 gcacctcggg cccggccccg ccggcgtcgg ggccggccag gccggcggcg gcatcgccgg
     1861 cggcgggcac ggccaggggc gggaccggcc agaccacctc cccgggcacc ccgaggacct
     1921 cgcggctggt ggccaggacc cggaccgccg ggccggaacg gagcagcccc tggaccagcg
     1981 cggcgcacgc cccgacaaga tgctcgcagt tgtcaaggac cagcagcagc gccttgtcgc
     2041 gggcgaagcc ggccaggcgc tcggccgggg acgggggcgg agcggcccgg tcggccgcct
     2101 cctcgcccag gcccagggcc gcgaccacga cctcggccag cagggccggg tcgcggaggg
     2161 gggccagctc gaccagccac accccgtccg ggtacccgcc catcagccgc cgggccagct
     2221 ccacggccac ggtggtcttg cccgctcctc cggggccggc caccgtgacc agccggtgct
     2281 gctccagcag cttggccaac tcggccagct cctggtcccg cccgaccagg ctggtcagcc
     2341 gctcgggcag gttgtgccgg ggcaggtccc ggtcacgggc ggccgcctca agggccgggt
     2401 cctggaccag gatcgcctgg tgcagccgct gcagctccgg cgacgggtcg atccccagct
     2461 cctcggccag cacctcgcgg gtctgctggt agacggccag ggcgtcggcc tgccggcccg
     2521 accggtacag ggccaccatc agctggcccc ggagccgctc ccgggtcggg tgggcggcaa
     2581 ccagcgcctc cagctccccg accagcccgg cgtggccgcc ggcggccagg cgcagctcga
     2641 ccagggcctc gcgggcggcc aggcgcagct cctccagccg ggccgcctcg gcctgggccc
     2701 acggctggtc ggcgaactcg gccagggccg ggccgcgcca cagccccagc gcctcctcca
     2761 gcagccgcac cgcccgcgga ggctccgccg gcgcggccgc ctcggcctcg gcgaccaggc
     2821 gctcgaacct ggtcgcgtcc agctgctcca ggccggcgtc cagccggtac ccgggcgggc
     2881 tggtgaccag ccgccccgcc ccgcccggcc ccagggcccg gcgcagggcc gacacccggc
     2941 cctgcagggt gttggccggc cgccccggcg ggtcgtcgcc ccacaggtcc tcgaccagcc
     3001 ggtccgccga caccacccgg ccggcgttga ccagcaggtc ggccagcagg gcccgctcct
     3061 tggcccccgg cacccgcacc ggcgcctccc cgttgcggac ctcgagaggc cccaggatcc
     3121 cgaactgcat ctccgccttc ccgcagcaaa cgcacagcgc cacgtcaggg cgccggccca
     3181 tcgtggcatc agcaccggtc agcgaccaag gaggattgcg atgcaggaac gtgaggtgcg
     3241 ggagctgttc gagcggctcc ggacggccat ggaccccgcg atcgagtacg aggagcggca
     3301 cgaggactac ctcgtcgagt tcccccagtc gggcgagcgg ctggaccgcg acggcctgcg
     3361 ccggctccag gagaccttcc cgggcggcac gccgcccagg atccggctgc gccggctgac
     3421 cggcggcggg gacgtctggt tcgccgagag cgccatccac tacgccgacg gcaaggactt
     3481 cttcggcgtg agccgggtcg agttccgcga cggcaagatg tggcgcgaga cccgctacta
     3541 cgccgagccg ttcgaggtcc cggagtggcg ggccgagtgg tccaatccgg tcctcgagga
     3601 ggcggccagg tgagctagcc ggccgcggcc agggcgcggt cgtcggcggt gaagtcggcg
     3661 tccgcgccct ccggtgggtt ggccaggtgc caggccaccg agcggtggag ggcctcgtgg
     3721 gggtcggtct cggtccagcc caggaggtcg cgggccttgg aggagtcgac caggaggtgc
     3781 tgggagacgg tgccgagggc cttgaggtcg tcgggcagga ggacgtcggg gacccggacc
     3841 agctcggcct cggagccggc ggcctccagg acgtggcggg cccacaggcc catcgaccag
     3901 gtgcgggcct cgccgaggtt gaggatctcg gccacgcagg cgtcggactc cagggccagg
     3961 cggatgccgg cggcgacgtc gcggacgtac ccgcgggtcc acaaccagcc gcccgacccg
     4021 gcggggatcc ggtcgcggcc ggcccggacc cggcgcagga gcggctcctc gcggcgctgg
     4081 tggtcgcgct cgccgtagac catcgggagc cggcacacgg tggccgcccg ggccaggtag
     4141 gcggcctcga cgtcgagctt ctcgtaggtg tcggcgtcgg tcgaggtgag ggtccggccc
     4201 cggtaggggt agcgctcgga gcggaccggg gaggtctcgt cgacgggcag ggcgtcggtc
     4261 tcggtgccgg ccagcaccgc cccgaacgcc cggtacacgt ccatgctgga caccaccagc
     4321 aggcgcacgt cgccgccgac ggcggcgagg gcggtctcgg cgtcggcggc cgagtaggcg
     4381 cagacgtcga ccacggcctc ggggtcgaac tcgtcgaccg ggccgcgcag gtggggcagg
     4441 tcctggcggt cggtgtggag atggtcggcg tctggaaggc cggccggctc gtgctcgccc
     4501 cggtggacga ccagcagctc gtggccatgg gcggccagct cctcgaccac ggcggccccg
     4561 acgaacctgg tcccgcccag caccatcacc cgcatcggcg cgctccttcc ctggtcagaa
     4621 cccgagccgg gcgagctggc gggggtcacg ctgccactcc ttggcaacct t
//
LOCUS       DASSWR010000047         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_595355,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000047 DASSWR010000000
VERSION     DASSWR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595355"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFO47_00810"
     CDS             <1..367
                     /locus_tag="VFO47_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8787270.1"
                     /translation="DTEGRFSVLEHRLDAHCPGPPVHVHDQLDHAMYVLEGTVVSTVE
                     QRQLTGPAGSFALVPHGIAHTSANPSDAVARLVEVDAPAGFERYFERLAEAFPAGSQL
                     DPATMVAIQAEHDTHPPHA"
     gene            584..1459
                     /locus_tag="VFO47_00815"
     CDS             584..1459
                     /locus_tag="VFO47_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787271.1"
                     /translation="MTDAGSSAPSDGKTITEYELEQCQRANQTGRPPVVFVHGLWLLP
                     NSWDRWAKLFEDNGYVALTPGWPDDPETVEEAAEHPEVFAGKSIGQVADHFETIIRGL
                     DRKPAIIGHSTGGLLTQILAGRGLAAVSVAIDPAPFRGVLPLPISTLKSSSPVLRNPA
                     NRNRAVPLTFEQFRYGFANAVSEDEAKQLYEDFAVPASGKALWQAAAANLNPWTEDKV
                     DTKNPDRGPLLLISGELDHTVPPAIVNASYKQQQDNPGVTEIEKIPGHGHALTIDSGW
                     REVADTALAFVQRFT"
     gene            complement(1472..1699)
                     /locus_tag="VFO47_00820"
     CDS             complement(1472..1699)
                     /locus_tag="VFO47_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787272.1"
                     /translation="MEEGYTQLGTVLTDQRPDDSEGDGVIVVGRFKGDPYDGVQLSYD
                     AGRRNLYLTPEGALRLAFLLAAAVERDIDIR"
     gene            complement(2271..2352)
                     /locus_tag="VFO47_00825"
     tRNA            complement(2271..2352)
                     /locus_tag="VFO47_00825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2316..2318),aa:Leu,seq:tag)
BASE COUNT          379 a          890 c          790 g          352 t
ORIGIN      
        1 cgacaccgag gggcgcttca gcgtcttgga gcatcgactc gacgcccact gtcccggccc
       61 tcccgtgcac gtgcacgacc agctggatca cgccatgtac gtcctggagg gcacggtcgt
      121 gtccaccgtg gagcagcgac agctcaccgg cccagccggc agcttcgccc ttgttcccca
      181 cgggatcgcc catacctccg ccaacccaag cgacgccgta gcgcggttgg tggaggtgga
      241 cgcccccgcc ggcttcgaac ggtactttga gcgcctggcc gaggcgtttc ctgccggcag
      301 ccagctcgac cccgccacca tggtggcgat ccaggccgag cacgacaccc acccgccgca
      361 cgcctgaccg ctgtcgcctg gggcaggtca gacccgccag gcagtatcga ggaacggcgg
      421 cacgtcgcag agccgtcgta gcgacccagt tgacagacct agcagcctgg taaccccgcc
      481 accccggccg ccaggtgcag tcatctgact gtagtcagca ccggcgtcgt ccctctatgg
      541 tggggtgtcg gttgcttgtc ccgcgtgagc gaggagaact gtcatgaccg atgcagggtc
      601 gagcgccccg tcggacggca agacaatcac cgagtacgag ctggagcagt gccagcgcgc
      661 caaccagacc gggcgcccgc cggtggtgtt cgtccacggc ctgtggctgc tgcccaacag
      721 ctgggaccgc tgggcgaagc tgttcgagga caacggctat gtggccctga cccccggctg
      781 gccggacgac cccgagacgg tcgaggaggc cgccgagcac cccgaggtgt tcgccggcaa
      841 gtcgatcggc caggtcgccg accacttcga gacgatcatc cgcgggctgg accgcaagcc
      901 ggcgatcatc ggccactcca ctggcgggct cctgacccag atcctggccg ggcggggcct
      961 ggccgcggtg tcggtggcca tcgacccggc ccccttccgg ggggtgctgc ccctgcccat
     1021 ctcgaccctc aagtccagct caccggtgct gcgcaacccg gccaaccgca accgggccgt
     1081 gccgctcacc ttcgagcagt tccgctacgg cttcgccaac gccgtcagcg aggacgaggc
     1141 caagcagctg tacgaggact tcgcggtgcc ggcctccggc aaggcgctgt ggcaggcggc
     1201 ggcggccaac ctgaatccct ggaccgagga caaggtcgac accaagaacc ccgaccgtgg
     1261 gccgctgctg ctcatctccg gcgagctgga ccacaccgtc cccccggcga tcgtcaacgc
     1321 ctcctacaag cagcagcagg acaacccggg cgtgaccgag atcgagaaga tccccggcca
     1381 cggccacgcg ctgaccatcg acagcggctg gcgcgaggtc gccgacaccg ccctcgcgtt
     1441 cgtccagcgc ttcacctagt ggtgtggatc cctaccggat gtcgatatcc ctttcgaccg
     1501 ccgccgcgag caggaatgcc aggcgcagcg ccccctctgg ggtcaggtac aggtttcgcc
     1561 ggccagcgtc gtagctgagc tgaacgccgt cgtaggggtc gcccttgaag cggccgacga
     1621 cgatgacgcc atctccctca ctgtcgtcgg gacgctgatc tgtcagcacg gtgccaagct
     1681 gcgtgtagcc ctcttccatg tgctgatcct ctggtgatcg tcgcgaccct ggctttgcgc
     1741 tggggtcgcg ctgcgttcgg gtgcgcctcg tgcggccagc ccggccatca tgccgaccac
     1801 ctggcgccgt cgcctgcgtc ctcggggcgg gctgacggcg ctccactcga ccttccagcc
     1861 agtgcaagtc cctaccgcca ccccggtcag caaggcgagc gttacgatcc gaccggcagg
     1921 ccagcgggcc agagtcaagc gcgatcgagg catgcggccg aactcctccg ctggtccgac
     1981 gtcaatctgg aggccggccg cgtcccaccg cggcggcccc gcgtcgtcgt gaactacgag
     2041 gtccacatcc cggagcccaa gacggccaag ggccggcgct cgctggccct cgacccggcc
     2101 acggtggcgg ccctgtgtca gcaccgcgcc cgccaggccg aggaacgcct ggccgtaggg
     2161 ccgcgttggc aggacccggg cctcggttca cctggccgga cggcagacca gtccatccgc
     2221 agcggttctc cacaaggagg tgaagcgcga agctcctggt cggaggggtg tgcgagcggg
     2281 gggattcgaa cccccacggg tcgccccacc gggacctaaa cccggcgcgt ctgccggttc
     2341 cgccacgctc gcgaactgcg gtcgagctta caacaggggg ttccggggcc ctgttcccct
     2401 caagtggcgg c
//
LOCUS       DASSWR010000048         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1301494, whole genome shotgun sequence.
ACCESSION   DASSWR010000048 DASSWR010000000
VERSION     DASSWR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301494"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFO47_00830"
     CDS             complement(<1..810)
                     /locus_tag="VFO47_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HET8787273.1"
                     /translation="MPTDKDFKRLVRARMGKTGESYTTARSHLRPDQGPDRPGRLRGR
                     HPDTAALARLLAALGVTDPATGRPLSEATVLGVSGGIGFAYFVFEYEEGATLYLGGRI
                     NSYVQKQDAAEAALTRLGVPFQAHRTSGPATAERQLRAALDQGRPVVATVDVARLRYR
                     AVPDWLCGMTPQDVLVELADGEPLLWDLAPAPFPVTWAELAEARAGVRSAKHRLLVAE
                     PPEEPVDLAGAAAAGIADTWKGMLEPPMRNFGAPGLAKWADLLTDTRDPKGW"
     gene            complement(1020..1781)
                     /gene="hisF"
                     /locus_tag="VFO47_00835"
     CDS             complement(1020..1781)
                     /gene="hisF"
                     /locus_tag="VFO47_00835"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020170963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET8787274.1"
                     /translation="MTLARRVIPCLDVAAGRVVKGVSFVNLVDAGDPAELAAIYDAEG
                     ADELVFLDITATVEARATLFEAVARTAEQVFIPLTVGGGVRTVEDVQRLLRAGADKVS
                     VNSAAVRDPELLSAGAGTYGVQCMVRAIDARRRPGGDGWEVVVDGGRTPTGLDALAWA
                     EEGARRGAGELLVTSMDADGQKTGYDLELVRAVTGAVDLPVIASGGAGQVDHFAAAAQ
                     AGADAVLAASVFHFGQLSVAEVKQGLADAGLPVRA"
     gene            complement(1778..2548)
                     /locus_tag="VFO47_00840"
     CDS             complement(1778..2548)
                     /locus_tag="VFO47_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET8787275.1"
                     /translation="MTLELLPAVDLLDGQAARLVQGVAGTETGYGDPVEAATGFAAQG
                     ASWLHVVDLDAAFGRGPRNREHLGRIVQAVAPAVRVEASGGVRTADDLHALLELGAAR
                     VVIGTAALEDPAWVAEACARHGEAVAVGLDARGRTLQARGWTASGGDLFEVLERLEAA
                     GCRRFVHTEVARDGMLTGPATDRLGEVLAATSRPVIASGGVAQLGDLRALAALEPDGL
                     EGAIVGKALYAGRFTVAEALATLAEAAPGNGAPDGRRA"
     gene            complement(2545..3207)
                     /gene="hisH"
                     /locus_tag="VFO47_00845"
     CDS             complement(2545..3207)
                     /gene="hisH"
                     /locus_tag="VFO47_00845"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET8787276.1"
                     /translation="MTRPPVAVLDYGVGNLHSAAKALDRAGADVRVVPTVEAAAGAAG
                     LVVPGVGAYGACLTGLASAGGAAAVAGWLRGGRPLLGICVGMQLLFEASEEGPVADGV
                     GVVPGKIRRLTGGVRGTPRGCPGESGPVKIPHIGWDEVTVRPGSRLFAGLGDGTRFYF
                     VHSYAPEPDGDAVAAVCDYGGRFGAAVERGNLFGTQFHPEKSGRAGLALLANFVTQVG
                     AA"
     gene            complement(3204..3824)
                     /gene="hisB"
                     /locus_tag="VFO47_00850"
     CDS             complement(3204..3824)
                     /gene="hisB"
                     /locus_tag="VFO47_00850"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET8787277.1"
                     /translation="MIAGGVAAGPPRTARIERRTAEVSVTVELDLDGSGSGGSDTGVP
                     FLDHMLSQLGRHARFDLSIEAKGDLEIDAHHTVEDTGIAVAQALAEALGDKSGVRRFG
                     DAVVPLDEARARAAVDLSGRPYLVWDVQVDAAPGELGSFDPRQARHLMEALVANARCT
                     IHVQLEAGDLPHHCLEACFKALARALGDACAPDPRAGAGSTKGVLG"
     gene            complement(3821..>4068)
                     /locus_tag="VFO47_00855"
     CDS             complement(3821..>4068)
                     /locus_tag="VFO47_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8787278.1"
                     /translation="ERVAAAMDRLPGVRVLPSDANFLCFETPVDPRRLWQGLLDRGVL
                     IRDVSRYPTLDRHLRVTVGTPAEDDAFLTALTEELNR"
BASE COUNT          478 a         1510 c         1609 g          471 t
ORIGIN      
        1 ccagcccttg gggtcgcggg tgtcggtgag caggtcggcc cacttggcca gcccgggcgc
       61 gccgaagttg cgcatcggcg gctccagcat gcccttccag gtgtcggcga tcccggcggc
      121 ggccgccccg gccaggtcca ccggctcctc cgggggctcg gccacgagca ggcggtgctt
      181 ggccgagcgg acgccggccc gggcctcggc cagctcggcc caggtgaccg ggaacggcgc
      241 cggggccagg tcccacagca gcggctcacc gtcggccagc tcgaccagga cgtcctgggg
      301 agtcatgccg cagagccagt cggggacggc ccggtagcgc aggcgggcca cgtcgacggt
      361 ggcgacgacc gggcggccct ggtcgagcgc ggcccgcagc tgccgttcgg ccgttgccgg
      421 gccgctggtc cggtgcgcct ggaacgggac cccgaggcgg gtcagggcgg cctcggcggc
      481 gtcctgcttc tgcacatagg agttgatccg gccgcccagg tagagggtgg ccccctcctc
      541 gtactcgaac acgaagtagg cgaacccgat gcccccggag acgcccagga ccgtcgcctc
      601 gctcaggggc cggccggtcg ccgggtcggt gacgccgagg gcggcgagca gccgggcgag
      661 cgcggccgtg tccgggtgcc ggccccggag ccgaccgggc cggtcaggcc cctggtcggg
      721 gcgcaggtgg gagcgggcgg tggtgtacga ctcgccggtc ttgcccatgc gggcgcggac
      781 cagccgcttg aagtccttgt cggtcggcat gacccagtcc tctcaagtcg cgaggcccgg
      841 ccccccagca gccctggccg cgcgaccgga aggcaccggg cgccgcgcga tccgggagtg
      901 tgcccccctt tgcctctcca cgggccccgc tggggtgggc caccagaggt tgggcgctgg
      961 atcaccgcca ccggcaagct tgccgtggcc ggggtcgggg ggtcaaggcc cggcgtgggc
     1021 tacgcccgga ccgggaggcc ggcgtcggcc aggccctgct tgacctcggc gacggagagc
     1081 tggccgaagt ggaagacgct ggccgccagg acggcgtcgg cgccggcctg ggcggcggcg
     1141 gcgaagtggt cgacctggcc ggcgccgccg ctggcgatga ccgggaggtc gacggcgccg
     1201 gtcacggcgc ggaccagctc caggtcgtag ccggtcttct ggccgtcggc gtccatgctg
     1261 gtgaccagca gctcgccggc gccgcggcgg gcgccctcct cggcccaggc gagggcgtcg
     1321 aggccggtgg gggtgcggcc gccgtcgacc accacctccc agccgtcgcc gccgggacgg
     1381 cggcgggcgt cgatggcccg caccatgcac tggaccccgt aggtgccggc cccggccgac
     1441 agcagctcgg ggtcgcggac ggcggccgag ttgaccgaga ccttgtcggc cccggcccgg
     1501 agcaggcgct ggacgtcttc gacggtgcgg actcccccgc cgacggtcag ggggatgaac
     1561 acctgctcgg cggtccgggc gacggcctcg aacaaggtgg cgcgcgcctc cacggtggcc
     1621 gtgatgtcga ggaacaccag ctcgtcggcg ccctcggcgt cgtagatggc ggccagctcc
     1681 gccgggtcgc cggcgtcgac caggttgacg aacgacacgc ccttgaccac ccgcccggcg
     1741 gccacgtcca ggcacgggat cacccgccgg gccagggtca tgcccgcctc ccgtccgggg
     1801 cgccgtttcc gggggcggcc tcggccaggg tggcgagggc ctcggcgacg gtgaagcggc
     1861 cggcgtagag ggccttgccg acgatggcgc cctccaggcc gtcgggctcc agggcggcca
     1921 gggcccggag gtcgccgagc tgggcgacgc cgccggaggc gatcaccggg cggctggtgg
     1981 cggccaggac ctcgccgagg cggtcggtgg ccgggccggt gagcatgccg tccctggcca
     2041 cctcggtgtg gacgaagcgg cggcagccgg ccgcctccag ccgctccagg acctcgaaca
     2101 ggtcgccgcc gctggccgtc cagccgcggg cctgcagggt ccggccgcgg gcgtcgagcc
     2161 cgaccgcgac cgcctcgccg tggcgggcgc acgcctcggc cacccacgcc gggtcctcca
     2221 gggcggcggt gccgatcacc acccgggccg cgcccagctc cagcagggcg tgcaggtcgt
     2281 ccgcggtccg caccccgccg gaggcctcga cccggaccgc cggggcgacc gcctggacga
     2341 tccgtcccag gtgctcgcgg ttgcgggggc cgcgcccgaa ggcggcgtcc aggtcgacca
     2401 cgtgcagcca gctcgccccc tgggcggcga agccggtggc cgcctccacc gggtcgccgt
     2461 agccggtctc ggtgccggcg acgccctgga cgaggcgggc cgcctgcccg tcgagcaggt
     2521 cgaccgccgg gagcagctcc agggtcatgc ggcgccgacc tgggtgacga agttggccag
     2581 cagggccagg ccggccctgc ccgacttctc cgggtggaac tgggtcccga acaggttgcc
     2641 gcgctcgacg gcggcgccga agcggccgcc gtagtcgcag acggcggcga ccgcgtcgcc
     2701 gtccggttcg ggggcgtagg agtggacgaa gtagaagcgg gtcccgtccc cgaggccggc
     2761 gaacaggcgg ctgccgggcc ggacggtgac ctcgtcccag ccgatgtggg ggatcttcac
     2821 cgggcccgat tcaccggggc acccccttgg ggttccccgg acccctccgg tgaggcgccg
     2881 gatcttgccc gggacgaccc cgacgccgtc ggcgaccggg ccctcctcgc tggcctcgaa
     2941 cagcagctgc atgccgacgc agatgccgag cagcgggcgg ccgcctcgca gccagccggc
     3001 caccgcggcc gccccgccgg ccgaggccag cccggtcagg caggccccgt aggccccgac
     3061 cccgggcacg accagcccgg ccgccccggc ggccgcctca acggtcggca ccacccggac
     3121 gtcggcgccg gcccggtcca gggccttggc ggccgagtgc aggttcccga ccccgtagtc
     3181 gagcacggcg accggcggcc tggtcatccg aggaccccct tggtgctgcc ggcgccggcc
     3241 cgcgggtcgg gcgcgcaggc gtcgccgagg gcgcgggcca gggccttgaa gcacgcctcc
     3301 aggcagtggt ggggcaggtc gccggcctcc agctggacgt ggatggtgca gcgggcgttg
     3361 gcgaccagcg cctccatcag gtggcgggcc tggcgcgggt cgaagctgcc gagctcgccg
     3421 ggggcggcgt cgacctggac gtcccagacc aggtagggcc ggcccgacag gtcgacggcg
     3481 gcccgggccc tggcctcgtc gagggggacg acggcgtcgc cgaagcgacg cacgcccgac
     3541 ttgtcgccca gcgcctcggc cagggcctgg gcgacggcga tgccggtgtc ctcgaccgtg
     3601 tggtgggcgt cgatctcaag gtcgcccttg gcctcgatgg agaggtcgaa gcgggcgtgc
     3661 ctgcccagct gggagagcat gtggtcgagg aacggcacgc cggtgtcgga gccgccgctg
     3721 ccgctgccgt ccaggtcgag ctcgacggtc accgagacct cggcggtgcg ccgctcgatc
     3781 cgggcggtcc ggggcggccc tgcggccact ccccctgcga tcatcggttc agctcctcgg
     3841 tgagggcggt caggaacgcg tcgtcctcgg ccggggtgcc gaccgtgacc cgcaggtgcc
     3901 ggtccagggt cgggtagcgg gagacgtcgc ggatcaggac cccgcggtcg agcaggccct
     3961 gccacagccg gcgcgggtcg accggggtct cgaagcagag gaagttggcg tcgctgggta
     4021 ggacccggac cccggggagg cggtccatgg cggccgcgac ccgctccc
//
LOCUS       DASSWR010000049         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1160493, whole genome shotgun sequence.
ACCESSION   DASSWR010000049 DASSWR010000000
VERSION     DASSWR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160493"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1548)
                     /locus_tag="VFO47_00860"
     CDS             complement(49..1548)
                     /locus_tag="VFO47_00860"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HET8787279.1"
                     /translation="MRSPLEIRYRFGPLERRGWVGSLRPGQLAILASAVFFTILLVQA
                     LKSGLGLLGGVVLLSLGALLTFVPLYGRPLNEWVPVIGVFMVRKATGRSRFRSRAPEA
                     GTRFAGGDEPVSLPPSIGKVELLAFPFRGVELGVLADRPNHTFSAVIQARARSFVLLD
                     PVDKAHRLAGWAGVIAGLAREGSPISRVQWIERTAPDDPDALSRYLREAIDPSIGLDA
                     LPLQSYLRLTHAAAPVTEQHELYIVLQLNAGKAGRAIKQAGGKDTGACMVLARELEVM
                     ARRLESAEVEVLHALGPRRLAATIRLSYDPHARTNLARLDSVDPDRKGGVSARNAWPM
                     RAEEHWGYYRTNDVCHATYWIAEWPRIDVGPDFLAPLLVQTRSQRTVAVTMEPVPPLK
                     AMRAVGFAKTADVADEELRQKLGFLGTAKRRNQADAVARREQELADGHADVRFSGYIT
                     VTADSPEALAEACGEVEHAAGQSRLELQRCDGEQELCFSYTLPMGRGLK"
     gene            complement(1541..>2986)
                     /locus_tag="VFO47_00865"
     CDS             complement(1541..>2986)
                     /locus_tag="VFO47_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787280.1"
                     /translation="QDRRRAWTRAVLVFATLAALLVVLAPAALAQDQPAGSGQTQTTQ
                     PGGQDRCDTIPNPVARNVCRAGTNPGQAVTSAVSGAAQNVAETAGDSALRSFTAAVAA
                     AGKFFLEKVGGLIHGTTSPDVVNADWFASQYRVMLALAVVIALPILLVSVAQSIVRAD
                     GMQAIRSAFVFLPLAAILSAVGPAMAQMLINISDWMSAALGGNAAADAQKFMSDAGGA
                     LASLGAGSVNPAAPVFGILLGALVVTLGALSIWLELLLRAAAIYISVLFLPLALAAMI
                     WPAGWRWCRRLIEFLIAIIFAKVFIVAIINLAAAGLARGGLGDKFEGVLAGGALLLMA
                     AFTPIALLKLIPLAEAAVVTAGHQRAALRQATTGATALTTSSSVVSGMINTRFRSSAA
                     GPAAGFAAPAAAATAGAAVVGAARNRATSSFAAMSAAPEAEGGEPSPPAPIRSSARPS
                     PANFGGNGGASSAGTKPLKLPDLPGEKGSDA"
BASE COUNT          408 a          978 c         1230 g          370 t
ORIGIN      
        1 gccccaccgt aaccgtcgac ctacgctcgc ttcacaggct gcggtcgctc acttcagacc
       61 ccggcccatt ggcaacgtat aggaaaaaca aagttcctgt tcgccgtcgc agcgctggag
      121 ctccaggcgg gactggccgg cggcgtgctc gacctcgccg caggcttcgg cgagggcttc
      181 ggggctgtcg gcggtgacgg tgatgtagcc ggagaagcgg acgtcggcgt ggccgtcggc
      241 gagctcctgc tcgcggcggg cgacggcgtc ggcctggttg cggcgcttgg cggtgccgag
      301 gaagccgagc ttctggcgga gctcctcgtc ggcgacgtcg gcggtcttgg cgaacccgac
      361 ggcgcgcatg gccttcaggg gcgggaccgg ttccatggtg acggcgaccg tgcgctggga
      421 gcgggtctgg accagcaggg gggccaggaa gtcggggccg acgtcgatgc ggggccactc
      481 ggcgatccag taggtggcgt ggcagacgtc gttggtccgg tagtagcccc agtgctcctc
      541 ggcccgcatg ggccaggcgt tgcgggccga gacgcccccc ttgcggtcgg ggtcgacgct
      601 gtccaggcgg gccaggttgg tccgggcgtg ggggtcgtag gagaggcgga tggtggcggc
      661 caggcggcgg gggccgaggg cgtggaggac ctcgacctcg gccgactcga ggcggcgggc
      721 catgacctcc agctccctgg ccaggaccat gcaggcgccg gtgtccttgc cgccggcctg
      781 cttgatggcc cggccggcct tgccggcgtt gagctggagg acgatgtaga gctcgtgctg
      841 ctcggtcacc ggggcggcgg cgtgggtgag gcgcaggtag ctctgcaggg gcagggcgtc
      901 gaggccgatg gacgggtcga tcgcctcgcg caggtagcgg ctgagggcgt cggggtcgtc
      961 gggggcggtg cgctcgatcc actggacccg gctgatcggg ctgccttcgc gggccaggcc
     1021 ggcgatcacc ccggcccagc cggccagccg gtgggccttg tcgacggggt cgaggagcac
     1081 gaaggagcgc gcccgggcct ggatgacggc cgagaaggtg tggttgggcc ggtcggcgag
     1141 cacgcccagc tcgaccccgc ggaaggggaa ggcgagcagc tcgaccttgc cgatcgaggg
     1201 ggggaggctg acgggctcgt caccgccggc gaaccgggtg ccggcctcgg gggcgcgcga
     1261 gcggaagcgg ctgcgtccgg tcgccttgcg gaccatgaag actccgatca cggggaccca
     1321 ctcgttgagg ggccgtccat agaggggcac gaaggtgagc agcgccccga ggctgagcag
     1381 gaccacgcca cccagcagcc cgaggccgct cttgagggcc tggaccagca ggatggtgaa
     1441 gaacacggcc gaggccagga tggccagctg gccggggcgg agtgagccga cccagccgcg
     1501 gcgctccagc gggccgaagc ggtagcggat ctccagcggg ctacgcatcc gaccccttct
     1561 cgccggggag gtccggcagc ttcagcggct tggtgccggc gctggaggcg ccgccgttgc
     1621 cgccgaagtt ggccggggac gggcgggccg aggagcggat cggggccggg ggcgacggct
     1681 ccccgccctc ggcctcgggg gccgcgctca tggcggcgaa gctcgaggtg gcgcggttgc
     1741 gggcggcccc gacgaccgcc gccccggcgg tggcggccgc cgccggggcc gcgaagccgg
     1801 ccgccgggcc ggccgcggag gagcggaagc gggtgttgat catgcccgag accaccgagg
     1861 agctggtcgt gagggcggtc gcgcccgtgg tcgcctggcg cagggcggcc cgctggtggc
     1921 cggccgtgac caccgccgcc tcggcgagcg ggatgagctt gagcagggcg atcggggtga
     1981 aggcggccat cagcagcagg gccccgccgg ccaggacgcc ctcgaacttg tcgcccagcc
     2041 cgccccgggc cagcccggcc gcggccaggt tgatgatggc cacgatgaac accttggcga
     2101 agatgatggc gatcaggaac tcgatcaggc gccggcacca gcgccagccg gccggccaga
     2161 tcatggccgc cagggccagg ggcaggaaca gcaccgagat gtagatggcg gcggcccgca
     2221 gcagcagctc cagccagatc gacagggccc cgagggtgac gacaagggcg ccgagcagga
     2281 tcccgaacac gggcgcggcc gggttgacgg agccggcgcc gaggctggcc agggcgccgc
     2341 cggcgtcgct catgaacttc tgggcgtcgg cggcggcgtt gccgccgagg gcggcgctca
     2401 tccagtcgct gatgttgatg agcatctggg ccatggccgg gccgacggcc gacaggatgg
     2461 cggccagggg caggaacacg aacgccgagc ggatggcctg catgccgtcg gcgcgcacga
     2521 tcgactgggc caccgacacc agcaggatgg gcagggcgat gaccacggcc agggccagca
     2581 tcacccggta ctgggaggcg aaccagtccg cgttgaccac gtccgggctg gtggtgccgt
     2641 ggatcaggcc gcccaccttc tcgaggaaga acttgccggc ggcggccacc gcggcggtga
     2701 agctgcgcag ggcgctgtcg ccggcggtct cggccacgtt ctgggccgcc ccggacaccg
     2761 cgctggtgac cgcctggccc gggttggtcc cggcccggca gacgttgcgg gccaccgggt
     2821 tggggatggt gtcgcagcgg tcctggccgc ccggctgggt cgtctgggtc tggccggagc
     2881 cggccggctg gtcctgggcc agggcggccg gggccagcac caccagcagg gcggcgaggg
     2941 tggcgaagac caggaccgcg cgggtccagg cgcgcctacg gtcctg
//
LOCUS       DASSWR010000050         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_510343,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000050 DASSWR010000000
VERSION     DASSWR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510343"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..927
                     /locus_tag="VFO47_00870"
     CDS             64..927
                     /locus_tag="VFO47_00870"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8787281.1"
                     /translation="MILVTGATGTTGGELVRLLAEAGAPVRALVRSPDKAASIQRLGV
                     EVAVGDFEQPDTLDAAMAGCDHLFLLSPPSPHQPEQERNAIDAAQRVGVGHVVALSVL
                     GSNPDASVAFGRWHGEIDRYLAESGLPHTLLLPSGFMQNLLASAQYVADQGILYGMTG
                     ETRVSYIDTRDVAAVAARVLTSPGHQGKAYALTGPEALSGDEVAERLSAATGHPVGSV
                     DVPADTFGQALVGAGLPGWLVERLIELNIMMADGHAAGVTDEVARLTGRQPRTFAQFA
                     AEHRAVFGGQQ"
     gene            1117..2238
                     /locus_tag="VFO47_00875"
     CDS             1117..2238
                     /locus_tag="VFO47_00875"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET8787282.1"
                     /translation="MDEGRHDPGRQAARRRMRLLTTVGGAEGVTPVTQRNLGVWLALG
                     LIAVIVGLDMVGGGNAELLGLLVCPPILAASFVGPKRTVAVGLVALAVAVGYGRAVGV
                     DLLAGAQGVPVVAIGVATVLSALVARVRVEREGRLRAVTRVAEVAQRALLGGVPPALG
                     SLRLAVLYASASAEASIGGDFYEALETPHGVRLLVGDVRGKGLDAVRLAGVVLGSFRD
                     AAQDYPDLAQVTAALDRSVTRAIGPEDFVTAVLVQVAIDGSAIVVNCGHPAPLLVRGG
                     RATALDPPEPAPPLGLGPRPEPLRLRLALGDRILLYTDGASEARARGGFFPLQTAAAF
                     ALVSADLEAGLETLWAELQRYVAGQLHDNIALLLIEHSR"
BASE COUNT          296 a          797 c          930 g          361 t
ORIGIN      
        1 cggcgtccgc ggcaggatgg gggtggagac cccagaggat cgccgcacgg tatggaggag
       61 cccgtgatct tggtcaccgg tgccaccggc accaccgggg gggagctggt gcggctgctc
      121 gctgaggccg gcgcgccggt ccgggccttg gtccgctcgc ccgacaaggc cgcctcgatc
      181 cagcgcctgg gcgtggaggt cgctgtgggt gacttcgagc agcccgacac cctggacgcc
      241 gctatggccg gctgcgacca cctgttcttg ctgtcgccgc ccagccccca ccagcccgag
      301 caggaacgca atgccatcga cgcggcccag cgggtcgggg ttgggcatgt ggtcgcgttg
      361 tcggtgctgg ggtccaaccc ggacgcctcg gtggcgtttg ggcgctggca cggcgagatc
      421 gaccggtacc tggccgagtc gggcctgccc cacacccttt tgctgccgag cgggttcatg
      481 cagaacttgc tggcgtcggc gcagtatgtc gccgaccagg gcatcttgta cggcatgacc
      541 ggcgagaccc gggtcagcta catcgacacc cgcgacgtcg ccgcggtggc cgcccgggtg
      601 ctgaccagcc ccggccacca gggcaaggcg tacgcgctga ccgggccgga ggccctgtcg
      661 ggggatgagg tggccgagcg gctgtcggcg gccaccggcc acccggtcgg ctcggtcgat
      721 gtcccggccg acaccttcgg ccaagcgctg gttggcgctg gcctgcccgg ttggctggtc
      781 gagcgcctga tcgagctcaa catcatgatg gcggacggcc acgccgctgg ggtcactgac
      841 gaggtggcca ggctgaccgg acgccaaccg cggacctttg cgcagttcgc cgccgagcac
      901 cgcgctgtct tcggcggcca gcagtagtac ctgccagcgg tggcgcagtt gccgcctggc
      961 ttgggcggtg gccgaccgca gatccacgct ggagctggcg gcaggagtcg gtccctcgac
     1021 ctgctcgacc aggtcaccgc tggctcccgt ggttgtcttg accaagctga ggccacaaga
     1081 tcaacccagg aaacggcgca ggtcgggagc aagatgatgg acgagggcag gcacgatcca
     1141 ggccggcagg ccgccaggcg ccggatgcgc ctgctcacca cggtgggcgg cgccgagggg
     1201 gtcacgccgg tgacccagcg caacctcggg gtgtggctgg cgctggggct tatcgcggtg
     1261 attgttggcc tggacatggt cggtggcggc aacgcggagc tgctggggct gctggtctgc
     1321 ccaccgatcc tggccgcctc gttcgtcggt cccaaacgca cggtcgcggt cgggctggtc
     1381 gcgctggccg tcgcggtcgg ctacggccgg gcggtcgggg tggacctgct ggccggcgcc
     1441 cagggggtgc cggtggtcgc gatcggggtc gcgaccgtgc tgtcggcgct ggtggcgcgg
     1501 gtccgggtgg agcgggaagg gcggctacgg gcggtcaccc gggtcgctga ggtcgcccag
     1561 cgggcgctgt tgggcggggt gcccccggcg ttggggagcc tgcgcctggc cgtgctgtac
     1621 gcctcggcca gcgccgaggc gtcgatcggg ggggacttct atgaggcgct ggagaccccg
     1681 cacggggtac ggttgctggt cggggacgtg cgcggcaagg gcctggacgc ggtccggctg
     1741 gccggggtgg tgttgggcag cttccgggac gccgcccagg actaccccga cctggcccag
     1801 gtcaccgcgg cgttggaccg cagcgtgacc cgcgccatcg gcccggagga ctttgtgacc
     1861 gcggtgctgg tccaggtcgc catcgacggc agcgcgatcg tggtcaactg cggccatccc
     1921 gcgccgctgc tggtccgcgg cggccgggcc accgcgctgg acccacccga gccggcgccg
     1981 ccgctggggc tgggcccgcg cccagagccg ctgcggctgc ggttggcgtt gggcgaccgg
     2041 atcttgctgt acaccgacgg ggcgagcgag gcacgcgccc gcggcgggtt cttcccgttg
     2101 cagaccgcgg cagcgttcgc actggtcagc gccgacctcg aagccgggct ggagacgctc
     2161 tgggccgagc tgcaacgcta cgtcgctggg cagctccacg acaacatcgc tctgctgctc
     2221 atcgaacaca gccgctaaca gcaccccgct agggtggggt tgaccgcccc gctcccgatg
     2281 ctcgccctcg gggcttgcgg acacagcggc tcgtgggtta cggcgagatg atggcggtga
     2341 ggaggctggg atgaacggtg tcgatcggct gctggggtcg gtcg
//
LOCUS       DASSWR010000051         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_114905,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000051 DASSWR010000000
VERSION     DASSWR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114905"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFO47_00880"
     CDS             complement(<1..671)
                     /locus_tag="VFO47_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8787283.1"
                     /translation="MNQWLDPARPVPDLRTEVPGPEGRKVLARDAAVTSPSLPRAYPM
                     VPRRGSGALIEDVDGNLFLDFNAGIAVCSTGHAHPRVVEAVGRQAADLLHYSASDFYL
                     PVYSEYAEALAATAPMPGPVRAFLTNSGTEAVEGALKLARAATGRQYVVSFYGAFHGR
                     SYGSVSLTASKAKYHAGFGPLLPGVLHAPYGYCHRCPLNLTFPDCGHATVDWLEQTLF
                     RYEVDP"
     gene            complement(722..>2177)
                     /locus_tag="VFO47_00885"
     CDS             complement(722..>2177)
                     /locus_tag="VFO47_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8787284.1"
                     /translation="QTVPTVVDGEARSDGEPFTLSSPNDAGLELAEVHSATERDVDDA
                     VRAAQAAFGAWSATPWAERVALLRRAADLISERSNELAALMTMEVGKNRLESLGDVEE
                     AADLIRYYCQQLADNDGFAHKMGQLSANEHTRSVLKPYGVWAVISPFNFPMALAAGPA
                     GGALVAGNTVVLKPSPQGSFTGYRLYECLRDAGLPAGVFHFLPGGDEVGKLVAGHEGV
                     DGITFTGSYEVGMKIYRQYSKGVPRPAVCEMGGKNPAIVSAKADLDLAAEGVLRSAFG
                     FSGQKCSACSRVYVERAVAGEFTGRLADRAAQLRVGDPLDREVYMGPVIDREAVERFK
                     EAVKEARKEGRVLAGGEVLTDDLPKGNYVAPTVVGDLPTDHRLFKDELFVPFVAVAPV
                     DSLDEALKLANDTEYGLTAGFYSADQAEVDQFLDRIEAGVVYVNRKAGATTGAWPGVQ
                     PFGGWKGSGSSGKAGGGLYYVQQFMREQSQTVIA"
BASE COUNT          288 a          766 c          818 g          305 t
ORIGIN      
        1 gggtcgacct cgtagcggaa cagggtctgc tccagccagt cgacggtggc gtggccgcag
       61 tcggggaagg tgaggttgag cgggcagcgg tggcagtagc cgtagggggc gtgcagcacc
      121 cctgggagca gcggcccgaa cccggcgtgg tacttggcct tggaggcggt caggctgacc
      181 gagccgtagc tgcggccgtg gaaggcgccg tagaagctga ccacgtactg gcggccggtg
      241 gccgcccggg ccagcttgag ggccccctcc acggcctcgg tgccggagtt ggtgaggaag
      301 gcccgcaccg ggccgggcat gggtgcggtg gcggccaggg cctcggcgta ctcggagtag
      361 acgggcaggt agaagtcgct ggccgagtag tgcagcaggt ccgcggcctg gcggccgacc
      421 gcctcgacca cccgggggtg ggcgtggccg gtcgagcaga cggcgatgcc ggcgttgaag
      481 tcgaggaaca ggttgccgtc gacgtcctcg atcagggccc cgctcccccg ccggggcacc
      541 atcgggtacg cccggggcag cgacgggctg gtgacggccg cgtcccgggc cagcaccttg
      601 cggccctcgg gccccggcac ctcggtcctg aggtccggca ccggccgggc cgggtccagc
      661 cattgattca ccggggcacc cccctcgggg ttccccgggc ccctccgggt cgcaggctgg
      721 gtcaagcgat caccgtctgg gactgctcac gcatgaactg ctggacgtag tagaggccgc
      781 cgccggcctt gccggagctg cccgacccct tccagccgcc gaacggctgc acccccggcc
      841 aggcgccggt ggtggcgccg gccttgcggt tgacgtagac gaccccggcc tcgatccggt
      901 ccaggaactg gtcgacctcg gcctggtcgg cggagtagaa gccggccgtg aggccgtact
      961 cggtgtcgtt ggccagcttc agcgcctcgt ccagggaatc gaccggggcc acggccacga
     1021 acggcacgaa cagctcgtcc ttgaacagcc ggtggtcggt cggcaggtcg ccgaccacgg
     1081 tcggggcgac gtagttgccc ttgggcaggt cgtcggtgag gacctcgccg ccggccagga
     1141 cccggccctc cttgcgggcc tccttgaccg cctccttgaa ccgctccacg gcctcccggt
     1201 cgatcaccgg gcccatgtag acctcgcggt ccagcgggtc gccgacccgc agctgggccg
     1261 cccggtcggc caggcggccg gtgaactcgc cggccacggc ccgttccacg tacacccgcg
     1321 agcaggccga gcacttctgc ccggagaagc cgaaggccga gcggagcacg ccctcggcgg
     1381 ccaggtcgag gtcggccttg gccgagacga tggccgggtt cttgccgccc atctcgcaca
     1441 ccgccggccg gggcacgccc ttggagtact gccggtagat cttcatcccg acctcgtagc
     1501 tgccggtgaa ggtgatgccg tcgacgccct cgtggcccgc gaccagcttg ccgacctcgt
     1561 ccccgccggg caggaagtgg aacaccccgg ccggcaggcc ggcgtcgcgc aggcactcgt
     1621 acagcctgta gccggtgaac gagccctgcg gggacggctt gaggacgacg gtgttgccgg
     1681 cgaccagggc gcccccggcc ggcccggcgg ccagggccat ggggaagttg aaggggctga
     1741 tgaccgccca cacgccgtac ggcttgagca ccgagcgggt gtgctcgttg gccgacagct
     1801 ggcccatctt gtgggcgaag ccgtcgttgt ccgccagctg ctggcagtag tagcggatca
     1861 ggtcggccgc ctcctcgacg tcgccgaggg actccagccg gttcttgccg acctccatgg
     1921 tcatcagggc ggcgagctcg ttggagcgct cgctgatcag gtcggcggcc cggcgcagca
     1981 gcgccacccg ctccgcccat ggggtcgccg accaggcacc gaaggcggcc tgggcggccc
     2041 ggacggcgtc gtcgacgtcg cgctcggtgg ccgagtggac ttccgccagc tccaggccgg
     2101 cgtcgttggg gctggacagg gtgaacggct cgccgtcgct gcgggcctcg ccgtcgacca
     2161 ccgtggggac ggtctgg
//
LOCUS       DASSWR010000052         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_539014,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000052 DASSWR010000000
VERSION     DASSWR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539014"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2157
                     /gene="paaZ"
                     /locus_tag="VFO47_00890"
     CDS             133..2157
                     /gene="paaZ"
                     /locus_tag="VFO47_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HET8787285.1"
                     /translation="MIGSYVRGGWHTATDEGVTVRDASTGEPVGRVSSTGVDVAGMVE
                     HARGVGGPALRELTFHQRAAALKQLAGYLREHRDGLYQESARTGATLGDAKVDVDGGI
                     GTLFVYASKGRRELPDGVLYADGPPQRLGRGGTFVGQHVYSSRPGVAVFINAFNFPVW
                     GMLEKLAPAVLAGMPAIVKPASQTAYVTELAFRHLIESGALPPGSVQLLCGGVGDLLD
                     HLGGQDTVAFTGSAATARRLRAHPAVVGEAVRFNAEADSLNCSILGPDATPGDEEFDL
                     FVGEVVRELTEKTGQKCTAIRRCLVPAGQVRAVVDALGDRLAGVRVGDPRDEATTMGP
                     LVSRDQQAEVGRAVARLGRGAEVAVGGPGKVDLASGDPDRGAFFAPTVLVAGSPGTPE
                     LHQVEAFGPVTSVIGYQDAAQAVELAALGRGSLVGSVVTHDPDFARRVVAGVAPWHGR
                     LLVLDRDDAGESTGHGAPLPHLVHGGPGRAGGGEELGGIRAVLHHMQVTSVSASPAMV
                     TAVTGRWTTGAPTTGDATHPFRKHFEDLAVGETYRTPTRTVTMEDIERFAELSGDRFY
                     AHMDEAAAKANPFFEGRVAHGYFVVSAAAGLFVDPDPGPCLANYGLERLRFRKPVYPG
                     DTLAVALTCQQKTARDGEAYGEVRWDVTVTNQDDEPVATYEVLTLVATRV"
     gene            2199..3167
                     /gene="paaA"
                     /locus_tag="VFO47_00895"
     CDS             2199..3167
                     /gene="paaA"
                     /locus_tag="VFO47_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HET8787286.1"
                     /translation="MDGQTKEGQASDGQASFDAAIAADQRIEPRDFMPEEYRRTLVRQ
                     IAQHAHSEIIGMQPEGNWITRAPSLRRKAILMAKVQDEAGHGLYLYSAAETLGVSRDE
                     LMEALHSGRQKYSSIFNYPTLTWADMGAIGWLVDGAAIMNQVPLCRCSYGPYARAMIR
                     ICKEESFHQRQGFEILHTLSHGTEAQHAMAQDAVDRWWWPSLMMFGPPDDQSSHTAQS
                     MAWNIKRHSNDELRQKFVDMCVPQAEVLGLTLPDPELRWNEATQHHDFGPIDWDELFR
                     VIKGDGPVNRKRMEHKLRAHAEGAWVREAATAYAAKHRRDGREAAA"
     gene            3164..3466
                     /gene="paaB"
                     /locus_tag="VFO47_00900"
     CDS             3164..3466
                     /gene="paaB"
                     /locus_tag="VFO47_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HET8787287.1"
                     /translation="MSEREPEPSAGWPLWEVFVRARRGLSHNHVGSLHAPDAEMALRN
                     ARDVYTRRQEGVSIWVVPAGAITASSPDEKDPFFDPAGDKAYRHPTFYDVPGGVQM"
BASE COUNT          508 a         1321 c         1247 g          422 t
ORIGIN      
        1 ccgtacggat cgggcgcgag gctcgcaggt tcgcggcgag caagtcaacc ggtgccgggc
       61 aggttgacac cgccgacggg ctcccaaacc atgtgaccga atgttcggta gaaggaggac
      121 cacgtggcca ggatgatcgg ctcctatgtc cgcggcggct ggcacaccgc gaccgacgag
      181 ggagtgacgg tgcgggacgc gtccaccggg gagccggtgg ggcgggtgtc gagcaccggg
      241 gtggacgtgg ccgggatggt cgagcacgcc cgcggggtcg gcgggccggc cctgcgcgag
      301 ctgaccttcc accagcgggc ggccgccctc aagcagctcg ccggctacct gcgcgagcac
      361 cgcgacggcc tctaccagga gtcggcccgg accggggcca ccctgggcga cgccaaggtc
      421 gacgtcgacg ggggcatcgg gaccctgttc gtgtacgcca gcaagggccg ccgcgagctg
      481 cccgacggcg tcctctacgc cgacgggccg ccgcagcggc tgggccgggg cgggaccttc
      541 gtcggccagc acgtctacag ctcccggccc ggcgtggccg tgttcatcaa cgccttcaac
      601 ttccccgtct gggggatgct ggagaagctg gccccggccg tgctcgccgg catgccggcg
      661 atcgtcaagc cggccagcca gaccgcgtac gtgaccgagc tggccttccg gcacctgatc
      721 gagtcgggcg ccctcccgcc cgggtcggtg cagctgctct gcggcggcgt cggcgacctg
      781 ctcgaccacc tcggcgggca ggacaccgtc gccttcaccg gctcggcggc caccgcccgg
      841 cggctgcggg cccacccggc ggtggtcggc gaggcggtcc ggttcaacgc cgaggccgac
      901 tcgctcaact gctcgatcct cggccccgac gccaccccgg gagacgagga gttcgacctg
      961 ttcgtcgggg aggtggtccg cgagctgacc gagaagacgg ggcagaagtg cacggccatc
     1021 cggcgctgcc tggtcccggc cgggcaggtc cgggccgtgg tcgacgccct cggcgaccgg
     1081 ctggccgggg tgcgggtggg cgacccgcgg gacgaggcca cgaccatggg cccgctggtc
     1141 agccgcgacc agcaggccga ggtcggccgg gcggtggccc ggctgggccg cggcgccgag
     1201 gtcgccgtcg gcggccccgg caaggtggac ctggcctcgg gcgaccccga ccggggggcc
     1261 ttcttcgccc cgacggtgct ggtggccggc tcgcccggga cgcccgagct ccaccaggtg
     1321 gaggcgttcg ggccggtcac cagcgtgatc ggctaccagg acgccgccca ggcggtcgag
     1381 ctggccgcgc tcgggcgggg gagcctggtc ggctccgtcg tcacccacga ccccgacttc
     1441 gcccgccgcg tcgtcgccgg cgtcgccccc tggcacggcc gcctgctcgt cctcgaccgc
     1501 gacgacgccg gcgagtcgac cggccacggg gccccgctgc cgcacctggt ccacggcggg
     1561 cccggccggg ccggcggcgg cgaggagctg ggcgggatcc gggccgtcct ccaccacatg
     1621 caggtcacca gcgtctcggc ctcgccggcc atggtcacgg ccgtcaccgg ccgctggacc
     1681 accggcgccc cgaccaccgg cgacgccacc cacccgttcc gcaagcactt cgaggacctg
     1741 gccgtggggg agacctaccg cacccccacc cggacggtga ccatggagga catcgaacgc
     1801 ttcgcggagc tgtccggcga ccggttctac gcccacatgg acgaggccgc ggccaaggcc
     1861 aacccgttct tcgagggccg ggtggcccac ggctacttcg tggtgtcggc cgccgccggg
     1921 ctgttcgtgg accccgaccc gggcccctgc ctggccaact acggcctgga gcggctgcgc
     1981 ttccgcaagc ccgtctatcc cggcgacacc ctggcggtgg ccctgacctg ccagcagaag
     2041 acggcccggg acggcgaggc ctacggcgag gtccgctggg acgtcaccgt gaccaaccag
     2101 gacgacgagc cggtggccac ctacgaggtc ctcaccctgg tcgcgacccg cgtataataa
     2161 tcgaacgttc ggtaggtaag aggatcgggg agccccggat ggacggccag accaaggaag
     2221 gccaggccag cgacggccag gccagcttcg acgccgcgat cgccgccgac cagcgcatcg
     2281 agccacgcga cttcatgccc gaggagtacc gcaggacgct ggtccgccag atcgcccagc
     2341 acgcccactc cgagatcatc gggatgcagc ccgagggcaa ctggatcacc cgcgccccca
     2401 gcctgcgccg caaggccatc ctcatggcca aggtccagga cgaggccggc cacggcctgt
     2461 acctgtactc ggccgccgag accctcggcg tgtcccgcga cgagctgatg gaggccctcc
     2521 acagcggccg ccagaagtac tccagcatct tcaactaccc caccctgacc tgggccgaca
     2581 tgggcgccat cggctggctg gtcgacgggg cggcgatcat gaaccaggtc ccgctgtgcc
     2641 gctgctccta cggcccctac gcccgggcga tgatccgcat ctgcaaggag gagtccttcc
     2701 accagcggca gggcttcgag atcctgcaca ccctcagcca cggcaccgag gcccagcacg
     2761 ccatggccca ggacgccgtc gaccgctggt ggtggccgtc gctgatgatg ttcggccccc
     2821 ccgacgacca gtcgtcccac accgcccagt cgatggcctg gaacatcaag cgccacagca
     2881 acgacgagct gcgccagaag ttcgtcgaca tgtgcgtccc ccaggccgag gtcctcggcc
     2941 tgaccctgcc cgacccggag ctgcgctgga acgaggcgac ccagcaccac gacttcggcc
     3001 cgatcgactg ggacgagctg ttccgggtca tcaagggcga cggcccggtc aaccgcaagc
     3061 ggatggagca caagctgcgc gcccacgccg agggcgcctg ggtgcgggag gcggcgaccg
     3121 cctacgcggc caagcaccgc cgcgacggcc gggaggcggc ggcgtgagcg agcgcgagcc
     3181 ggagccgtcc gccggctggc cgctgtggga ggtgttcgtc cgcgcccggc gcggcctgtc
     3241 ccacaaccac gtcggcagcc tgcacgcccc cgacgccgag atggccctgc gcaacgcccg
     3301 cgacgtctac acccgccgcc aggagggcgt gtcgatctgg gtcgtcccgg ccggcgccat
     3361 caccgcctcc tcacccgacg agaaggaccc gttcttcgac ccggccggcg acaaggccta
     3421 ccgccacccg accttctacg acgtccccgg cggggtgcag atgtgagcac ggaggggtcc
     3481 ggccggaggg gtccgggg
//
LOCUS       DASSWR010000053         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_482103,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000053 DASSWR010000000
VERSION     DASSWR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482103"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFO47_00905"
     CDS             <1..214
                     /locus_tag="VFO47_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787288.1"
                     /translation="EIEAGATAAGDEEPETDEVEDEADEAPTGEADTAVEPGVTESGV
                     DEEEAASTQADDDAAETAGDEDQDRK"
     gene            230..961
                     /gene="truA"
                     /locus_tag="VFO47_00910"
     CDS             230..961
                     /gene="truA"
                     /locus_tag="VFO47_00910"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET8787289.1"
                     /translation="MRLVVAYDGAPFSGFARQRARRTVQGELEAALARLARQPVATVG
                     AGRTDAGVHARGQVVHADVPSRLDPDRVRRALNGGLGPVITVREAGWAPPGFDARLSA
                     RRRTYVYRVDDSGDPDPLLRGFVLAWPRPLDLPRMRQGARPLVGEHDFAAFCRSRSGA
                     TTTRRLRSIGIRRLRGLVEARLVADAFCWQMVRGIVGHLLLVGDGRRDPASTVTILSD
                     GDRSRAGNIAPPHGLVLEAVSYVRR"
     gene            complement(946..1725)
                     /locus_tag="VFO47_00915"
     CDS             complement(946..1725)
                     /locus_tag="VFO47_00915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006138172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8787290.1"
                     /translation="MTGAGPAAGVQGMVALVTGGSRGIGRAVAEEFAAQGATVVVQFR
                     ADGRAADDTLARLGAGGHRAAQADLADPGQARSLVARVVDELGRVDVLVNNAGIYEAQ
                     PILETGAEEWQRSWRRTIDTNLLGPANLIHAVAPHMVAAGGGRIVNVSSRGAFRGEPS
                     HPAYGASKAGLNSLGQSMARALGPHGIIVTTVAPGYVETDMAAPYLDGPGGDAIRAQS
                     PLNRVATPQEIARVVVFLATPGTESLTGAVVDVNGASYLRT"
     gene            complement(1722..2237)
                     /locus_tag="VFO47_00920"
     CDS             complement(1722..2237)
                     /locus_tag="VFO47_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8787291.1"
                     /translation="MRVEIGAGEKPDPGYEVHTDVLPLPGIQVLCRLDRLPFADGSVS
                     ALRANHVLEHQSFQLVEPTLREWARVLAPGARVDIGVPDARYIVTQWVEGGYSTLEAN
                     YWLLGGHMDREAHKGTDERGVPRWIWNAHHAMFDDAWLGQVLAANGFTDIDISCYDVR
                     NLRCYCRRGPR"
     gene            2333..>2846
                     /locus_tag="VFO47_00925"
     CDS             2333..>2846
                     /locus_tag="VFO47_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET8787292.1"
                     /translation="MPCACVAHVGSSAWERLPEALRLLRPRFGRVPVYPAGDPGDAET
                     LAAELAAEVDVLVVFGGDGTVHEVANGLPLGGDGPLVALLPAGTGNDLARALGMPPDP
                     VAAAGELADARPRTLDLLDCGPRRAANGVNAGFAASATDTLPRALKKALGPAAYVVGG
                     VRAGITPPTWP"
BASE COUNT          328 a         1123 c         1052 g          343 t
ORIGIN      
        1 cgagatcgaa gccggcgcca ccgccgccgg cgacgaggag cccgagaccg acgaggtcga
       61 ggacgaggcc gacgaggccc cgaccggcga ggccgacacg gccgtcgagc ccggcgtcac
      121 cgagtccggg gtcgacgagg aggaggccgc gtccacccag gccgacgacg acgccgccga
      181 gaccgccggg gacgaggacc aggaccgcaa gtagcccgaa gtgcggcgga tgcggctggt
      241 ggtggcgtac gacggggcgc cgttctccgg gttcgcccgg cagcgggccc gccggaccgt
      301 ccagggtgag ctggaggccg cgctggcccg gctggccagg cagccggtgg cgaccgtcgg
      361 ggcgggacgc accgacgccg gagtgcacgc ccgggggcag gtcgtccacg ccgacgtgcc
      421 gtcgcggctc gacccggacc gggtccggcg ggccctgaac ggcgggctgg ggccggtcat
      481 caccgtgcgc gaggccggct gggcgccgcc ggggttcgac gcccggctgt cggcccggcg
      541 ccgtacctac gtgtaccgcg tcgacgactc cggcgacccc gatccgctgc tgcgcggctt
      601 cgtgctggcc tggccccggc cgctcgacct gccccgcatg cgccaggggg cccggcccct
      661 ggtgggcgag cacgacttcg ccgccttctg ccgcagccgt tcgggggcca cgaccacccg
      721 gcggctgcgc tccatcggga tccgccggct ccgcggcctg gtcgaggccc ggctggtcgc
      781 ggacgcgttc tgctggcaga tggtccgcgg gatcgtcggc cacctcctgc tcgtcggcga
      841 cggccgccgc gaccccgcca gcacggtcac gatcctgagc gacggcgacc gttcccgcgc
      901 cggcaacatc gccccgccgc acggcctcgt gctggaggcg gtgagctacg tccgcaggta
      961 ggaggcgccg ttgacgtcga ccacggcgcc ggtgagggac tcggtccccg gggtggccag
     1021 gaagaccacc accctggcga tctcctgggg ggtcgccacc cggttgaggg ggctctgggc
     1081 gcggatggcg tcgccgccgg ggccgtccag gtacggggcg gccatgtcgg tctccacgta
     1141 gccgggcgcg accgtggtca cgatgatccc gtgcgggccg agcgcccggg ccatggactg
     1201 ccccaggctg ttcaggcccg ccttgctggc cccgtaggcg ggatggctgg gctcgccccg
     1261 gaacgccccc cgcgaggaga cattgacgat ccgccccccg cccgcggcca ccatgtgcgg
     1321 ggccacggcg tggatgaggt tggccgggcc gagcaggttg gtgtcgatgg tccgccgcca
     1381 gctccgctgc cactcctcgg cgccggtttc caagatgggc tgcgcctcgt agatcccggc
     1441 gttgttcacc agcacgtcga cccggccgag ctcgtccacg acccgcgcga cgagcgaccg
     1501 cgcctgcccg gggtcggcca ggtcggcctg ggccgcccgg tgcccgccgg ccccgagccg
     1561 ggccagggtg tcgtcggccg cccggccgtc ggcgcggaac tggacgacga ccgtcgcccc
     1621 ctgggccgcg aactcctcgg ccacggcacg gccgatcccg cgcgagccgc ccgtgaccag
     1681 cgccaccatg ccctggacac ccgccgccgg cccggccccg gtcatcgcgg cccccggcgg
     1741 cagtagcagc gcaggttgcg gacgtcgtag caggagatgt cgatgtcggt gaacccgttg
     1801 gcggccagca cctggcccag ccaggcgtcg tcgaacatgg cgtggtgggc gttccagatc
     1861 cagcggggca cgccccgctc gtcggtcccc ttgtgggcct cgcggtccat gtgcccgccc
     1921 agcagccagt agttcgcctc cagggtcgag tacccgccct ccacccactg ggtgacgatg
     1981 tagcgggcgt cgggcacccc gatgtcgacc cgcgcccccg gcgccagcac cctggcccac
     2041 tcgcgcaggg tcggctcgac cagctggaag ctctggtgct cgagcacgtg gttggcccgc
     2101 agggcgctga cgctcccgtc ggcgaacggc agccggtcga gccggcacag cacctggatg
     2161 ccggggaggg gcagcacgtc ggtgtgcacc tcgtagccgg ggtccggctt ctccccagcg
     2221 ccgatctcga cccgcaccga gccctcctcc cgacggccct tggcttgccg cgtggctgcc
     2281 accaccccgg ccaacgctaa cctcaccccg ccccatgtag ggtgccaggg agatgccatg
     2341 cgcctgcgtg gcccacgtcg ggtcgtccgc ctgggagcgc ctccccgagg ccctccggct
     2401 gctgcgtccc cggttcggcc gggtgccggt gtacccggcc ggcgaccccg gggacgccga
     2461 gaccctggcc gccgagctgg ccgccgaggt cgacgtcctg gtcgtgttcg gcggcgacgg
     2521 caccgtccac gaggtcgcca acgggctccc gctcggcggc gacggcccgc tggtcgccct
     2581 gctgcccgcc gggaccggca acgacctggc ccgggccctc ggcatgccgc ccgacccggt
     2641 ggccgcggcc ggcgagctgg ccgacgcccg cccccggacc ctggacctgc tcgactgcgg
     2701 cccgcgccgg gccgccaacg gcgtcaacgc cggcttcgcc gcctccgcca ccgacaccct
     2761 gccccgggcg ctcaagaagg ccctcggccc ggccgcctac gtggtcggcg gggtccgcgc
     2821 cgggatcacc ccgccgacct ggccgg
//
LOCUS       DASSWR010000054         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_340915,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000054 DASSWR010000000
VERSION     DASSWR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340915"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFO47_00930"
     CDS             <1..376
                     /locus_tag="VFO47_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET8787293.1"
                     /translation="PALAAYVSRDVAVGIRSEDMEDARLVRDAPADTRLKATVSLVEA
                     LGAQIVVHFDVDAPKVVTADTKLLDKDAHTEDVPTHVEGTKFVAAFAPRSRVRVGDQV
                     EVVVDTERMHFFDPETGAAIRD"
     gene            complement(373..1119)
                     /locus_tag="VFO47_00935"
     CDS             complement(373..1119)
                     /locus_tag="VFO47_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8787294.1"
                     /translation="MGDFDGTVVLVTGGASGIGAATADLLAERGARVAVLDLDPGPAG
                     GGHLRLACDVSDRAAVDRAVERAAGDLGGLDVVVNNAGIGAQGDVAANDDAEWARVLD
                     VNVVGIARVTRAALPHLRRSARAAVVNTCSVVATLGVPNRALYAASKGAVLALTLAMA
                     ADHVGDGIRVNCVTPGTVETPWVGRLLEAAGDPGAELAALRARQPLGRLVSAAEVAAA
                     IAYLAGPQAGATTGTALAVDGGMAGLRLPR"
     gene            1159..>2543
                     /locus_tag="VFO47_00940"
     CDS             1159..>2543
                     /locus_tag="VFO47_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET8787295.1"
                     /translation="MEEDGREVTPLRSAQWYGGSDRNAYIHRAWMRRGLPRHAFDGRP
                     HIAIANTASDLTPCNSHLDEVAASVKEGVWEAGGVPLNLPVVSLGETQVRPTAMLWRN
                     LAAMAAEELLRANPVDGAVLLGGCDKTIPALLMAAASVDLPALVVPGGPMLTGHFRGA
                     PLGCGTDVWRLGEEVRAGRLSRDTFLESESAMIRSRGHCNTMGTASTMACLAEALGTT
                     IPGVAGTPAPDSRLLERAHQSGRLAVELVAAGRRPSGFLGPGSFRNAIVALAAIGGST
                     NGVVHLLALAGRLGVDLSLEDFDRIGSGVPLLVDLQPAGRHLMDDFHRAGGLRAVLRE
                     VADLLDPDARTVTGRPLVEHLEDAELVDPEVIRPRDRPLKEAAGIAVLRGNLCPDGAV
                     IKPAAASPELLRHRGRAVVFDSIEDLHARLDDPALEVDETSVLVLRGGGPKGDPGMPE
                     VGNMPLPARLL"
BASE COUNT          308 a          999 c          937 g          299 t
ORIGIN      
        1 gccagccctg gccgcctacg tttcccgcga cgtcgccgtg gggatccgca gcgaggacat
       61 ggaggacgcc cggctggtcc gcgacgcgcc cgccgacacc cgcctcaagg cgaccgtctc
      121 gctggtcgag gccctcggcg cccagatcgt ggtccacttc gacgtcgacg cccccaaggt
      181 cgtcaccgcc gacaccaagc tgctggacaa ggacgcccac accgaggacg tccccaccca
      241 cgtcgagggg accaagttcg tggccgcctt cgccccccgc tcccgggtca gggtcggcga
      301 ccaggtcgag gtcgtggtcg acaccgagcg catgcacttc ttcgaccccg agaccggcgc
      361 cgccatccgc gactagcggg gcagccgcag gccggccatg ccgccgtcga cggccagggc
      421 cgtcccggtg gtggcgccgg cctgcgggcc ggccaggtag gcgatggcgg ccgccacctc
      481 ggcggcgctg accaggcggc cgagcggctg gcgggcccgc agggcggcca gctcggcccc
      541 ggggtcgccg gccgcctcca gcaggcgccc gacccagggc gtctcgaccg tgccgggggt
      601 gacgcagttg acccggatgc cgtcgccgac gtggtcggcg gccatggcca gggtgagggc
      661 gagcaccgcg cccttgctgg ccgcgtacag ggcgcggttg gggacgccca gggtggccac
      721 caccgagcag gtgttgacga ccgccgcccg ggccgagcgc cgcaggtgcg gcagggcggc
      781 ccgggtcacc cgggcgatgc cgacgacgtt gacgtcgagg acgcgggccc attccgcgtc
      841 gtcgttggcg gcgacgtcgc cctgggcgcc gatgccggcg ttgttgacca ccacgtcgag
      901 gccgccgagg tcgcccgcgg cccgctcgac ggcccggtcg accgccgccc ggtcgctgac
      961 gtcgcaggcc agccggaggt gcccgccgcc ggccgggccc gggtcgaggt cgagcacggc
     1021 cacacgcgcc ccgcgctcgg ccagcaggtc cgcggtggcg gccccgatcc cgctggcgcc
     1081 gcccgtgacc agcaccaccg tcccgtcgaa gtctcccatc ccaccctccc ggcgatccct
     1141 ctagactgag gcctgaatat ggaagaggac ggccgcgaag tcacgccgct gcgcagcgcc
     1201 cagtggtacg gggggtcgga ccgcaacgcc tacatccacc gggcgtggat gcggcggggg
     1261 ctgccgcggc acgccttcga cgggcggccg cacatcgcca tcgccaacac cgcctcggac
     1321 ctcacgccct gcaacagcca cctggacgag gtggcggcca gcgtcaagga gggggtctgg
     1381 gaggcgggcg gggtcccgct caacctgccg gtggtgtcgc tgggcgagac ccaggtgcgg
     1441 cccacggcca tgctctggcg caacctggcc gcgatggccg ccgaggagct gctgcgggcc
     1501 aacccggtcg acggggccgt gctgctcggc ggctgcgaca agacgatccc ggccctgctg
     1561 atggcggcgg cctcggtcga cctgccggcc ctggtcgtgc ccggcgggcc catgctcacc
     1621 gggcactttc gcggcgcccc gctcggctgc ggcaccgacg tgtggcgcct gggcgaggag
     1681 gtgcgggcgg gccggctgtc ccgggacacc ttcctcgagt ccgagtcggc gatgatccgc
     1741 agccgcggcc actgcaacac catgggcacc gcctcgacca tggcctgcct ggccgaggcc
     1801 cttgggacca ccatccccgg cgtcgccggc accccggcgc ccgacagccg gctgctggag
     1861 cgggcccacc agagcggccg gctggccgtc gagctggtcg ccgccgggcg ccgccccagc
     1921 ggcttcctgg ggccggggtc gttccgcaac gcgatcgtcg ccctggccgc catcggcggg
     1981 tcgaccaacg gcgtcgtcca cctgctggcc ctggccggcc ggctcggggt cgacctgtcg
     2041 ctggaggact tcgaccgcat cggctccggc gtgcccctgc tggtcgacct gcaaccggcc
     2101 ggccgccacc tgatggacga cttccaccgg gccgggggcc tgcgggccgt gctccgcgag
     2161 gtcgccgacc tgctcgaccc cgacgcccgc accgtgaccg gccggccgct ggtcgagcac
     2221 ctggaggacg ccgagctggt cgaccccgag gtgatccggc cccgcgaccg gcccctcaag
     2281 gaggcggccg ggatcgccgt cctgcgcggc aacctctgcc ccgacggggc cgtcatcaag
     2341 cccgccgccg cctcgcccga gctgctccgg caccggggcc gggcggtggt gttcgacagc
     2401 atcgaggacc tccacgcccg cctcgacgac ccggccctgg aggtcgacga gacctccgtg
     2461 ctggtcctgc ggggcggcgg ccccaagggc gacccgggga tgcccgaggt cggcaacatg
     2521 cccctgccgg cccggctgct gga
//
LOCUS       DASSWR010000055         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1301692, whole genome shotgun sequence.
ACCESSION   DASSWR010000055 DASSWR010000000
VERSION     DASSWR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301692"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFO47_00945"
     CDS             <1..1245
                     /locus_tag="VFO47_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET8787296.1"
                     /translation="GSAFKMFFVVAALEKGISPGLVLPGPAQITIPNRRCYTGYNQPW
                     TSGNAGDSSAGTYNMYGATANSVNTWFAQLAVRVGPERAVEVARRMGITNIPPRNSKA
                     YASWNVCSLVLGAREVSVLDMAGAFGVLANKGVRCAPYSITKVVAPGERKPLIENKPD
                     CKQVISEKISTRTVAMLRGVITNGTGGRASLGARPVAGKTGSAQNNTSAFFSGFTPQL
                     STSVWVGYRARRVPMTNLYNGSPVYGGTFPAIIFKNYMDAALAGQPAVGFPAAPPPPA
                     PPQVGVPDVVGLSQETAQAVLARAGFGSQVKPVRSAQPKGRVVRQAPGPGGRLRQGST
                     VILAVSAGRGGGGGGNNVIVPGVVGLRAGVARTVLTRAGLSSGLAYTNSGQPGRVAAQ
                     SPGAGAQLPRDSYITLVVSRRS"
     gene            complement(1242..1685)
                     /locus_tag="VFO47_00950"
     CDS             complement(1242..1685)
                     /locus_tag="VFO47_00950"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET8787297.1"
                     /translation="MLIDDVQQAMFQAMKAREARETAALRLALSALKSAAIDARKDLS
                     DDEAVAVLQREARKRREAEQVYRDAGRAERADQEAYERSVLERFLPAALDPAELARLV
                     DEAVTASGASGPREFGKVMGRLMPQVKGRADGNEVRRLVLERLGG"
     gene            1747..>2085
                     /locus_tag="VFO47_00955"
     CDS             1747..>2085
                     /locus_tag="VFO47_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8787298.1"
                     /translation="MPATTSKVALLAGAVAAAGAACVGYGVLVERDWYRLRRERVEAL
                     APGQAPLTVLHLSDLHLTAADTRQVAFLERLAGERVDLVVLTGDMLGEPGALDPVLDT
                     LGRFRPRLGAV"
BASE COUNT          272 a          791 c          750 g          272 t
ORIGIN      
        1 gggtcggcgt tcaagatgtt cttcgtcgtc gccgccctgg agaagggcat ctcgcccggg
       61 ctcgtcctgc ccggcccggc ccagatcacc atccccaacc gccgctgcta caccggctac
      121 aaccagccct ggacctccgg caacgccggc gactccagcg ccgggaccta caacatgtac
      181 ggggccacgg ccaactcggt gaacacctgg ttcgcccagc tcgcggtcag ggtcggcccc
      241 gagcgggcgg tcgaggtggc caggaggatg ggcatcacca acatcccgcc ccgcaacagc
      301 aaggcctacg cctcctggaa cgtctgctcg ctggtcctgg gggccaggga ggtctcggtc
      361 ctcgacatgg ccggggcgtt cggcgtgctc gccaacaagg gcgtccgctg cgcgccctac
      421 tcgatcacca aggtggtggc gccgggcgag cgcaagccgc tgatcgagaa caagcccgac
      481 tgcaagcagg tcatcagcga gaagatctcc acccggaccg tggccatgct gcggggggtc
      541 atcaccaacg gcaccggcgg cagggcgtcg ctgggcgccc ggccggtggc cggcaagacc
      601 ggcagcgccc agaacaacac cagtgccttc ttctccgggt tcacgcccca gctctccacc
      661 tcggtctggg tcggctaccg ggccaggcgg gtgccgatga ccaacctgta caacggcagc
      721 ccggtctacg gcgggacctt cccggcgatc atcttcaaga actacatgga cgcggccctg
      781 gccggccagc cggccgtcgg gttcccggcg gcgcccccgc ccccggcccc accgcaggtg
      841 ggcgtgcccg acgtggtcgg cctctcccag gagacggccc aggcggtgct ggcccgggcc
      901 gggttcggca gccaggtcaa gccggtccgc agcgcccagc ccaagggccg ggtggtccgc
      961 caggcgcccg gccccggggg ccgcctgcgc cagggcagca ccgtgatcct ggccgtctcc
     1021 gccggccgcg gtggcggtgg cggagggaac aacgtgatcg tgcccggggt ggtcgggctc
     1081 cgggccggcg tggccaggac ggtcctgacc cgggccgggc tgagctccgg cctggcctac
     1141 accaacagcg gccagccggg ccgggtcgcc gcccagagcc cgggcgccgg cgcccagctg
     1201 cccagggact cctacatcac cctggtggtg agccggcgga gctagccgcc gaggcgctcc
     1261 agcaccaggc ggcggacctc gttgccgtcg gcgcggccct tgacctgagg catgaggcgg
     1321 cccatgacct tgccgaactc cctggggccg ctggcccccg aggccgtgac cgcctcgtcg
     1381 accagccggg ccagctcggc cgggtcgagg gccgccggca ggaaccgctc cagcaccgac
     1441 cgctcgtagg cctcctggtc ggcccgctcg gcccgcccgg cgtcccggta gacctgctcg
     1501 gcctcgcggc gcttgcgggc ctcccgctgg agcacggcca cggcctcgtc gtccgacagg
     1561 tccttgcggg cgtcgatggc ggccgacttg agcgccgaca gggccaggcg cagggcggcc
     1621 gtctcccggg cctcgcgggc cttcatggcc tggaacatcg cctgctgcac gtcgtcgatg
     1681 agcatggtcc gagcctacag ctacaatcct cgttcatccc aggcagacct ccaggagcgc
     1741 gaccgcatgc ccgccaccac gtcgaaggtt gccctgctgg ccggggcggt ggccgccgcc
     1801 ggggcggcct gcgtcggcta cggcgtcctg gtcgagcggg actggtaccg gctgcgccgg
     1861 gagcgggtcg aggccctggc ccccgggcag gcgccgctca ccgtgctgca cctgtcggac
     1921 ctgcacctga cggccgccga cacccggcag gtggcgttcc tggagcggct ggccggcgag
     1981 cgggtcgacc tggtcgtgct gaccggggac atgctcggcg agcccggggc gctcgacccg
     2041 gtcctggaca cccttggacg gttccggccg cggctggggg cggtg
//
LOCUS       DASSWR010000056         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_30052,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000056 DASSWR010000000
VERSION     DASSWR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30052"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFO47_00960"
     CDS             <1..800
                     /locus_tag="VFO47_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET8787299.1"
                     /translation="DDLIVYREADGYLLVPNAANWPKVADAVRASDPGRTEVTPRPDI
                     AVLAVQGPRAPELVGGLFPEAADLPYMHLVATTYRDTTARVCRTGYTGERGFELLVPG
                     EVAPALWEELFERGAAFEVVPVGLGGRDTLRLEMGYALHGNDISTDTDPFEARLGWAV
                     AMDKGDFRGREALAARKEAGPKRLLVGLVAADRLIPRHGMAVLDGDRPVGEVTSGTFS
                     PTLRQGIALAYVLPDVAAKGTELQVDVRGKRGQVTVTRPPFVGSSPR"
     gene            803..1342
                     /gene="idi"
                     /locus_tag="VFO47_00965"
     CDS             803..1342
                     /gene="idi"
                     /locus_tag="VFO47_00965"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017563268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET8787300.1"
                     /translation="MATGVGSELVVLLDEDGRPAGTMPKARVHGPDTPLHLAFSVYLF
                     DAGGRFLATRRALGKRTWPGVWTNSCCGHPAPGEEPADAARRRLGQELGLHPARMELV
                     LPSFAYRAVAADGVVENEVCPVFFAHLDGDPEPPPTPDPEEVAEWRWVPWAAFRAVAT
                     TAPWALSPWSVEQVRQLPA"
     gene            1383..>2151
                     /locus_tag="VFO47_00970"
     CDS             1383..>2151
                     /locus_tag="VFO47_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HET8787301.1"
                     /translation="MARHDPDFLEFFAAEFWPLRRVGFLLTGDWDQAEELAQEAMART
                     YAVWPRVRGYDRPAAYARKVLLNRHRSLLRRAVVEARHLLTSHPDDRHQPDFGGDDLV
                     LWQALQGLPSRQRAALVLRFYLDLPEAEVARLLGVPAGTVKSWTHRGLARLRDRLGPT
                     YADERLAGNPSTEAGNFHFRYPPGWEARRIGGQWALTLRGIPRSDQGVPEFEVRVTSA
                     RSLWRSHGYWQGTAPEVGRLPAGQAYLLKSHAPAEYST"
BASE COUNT          254 a          861 c          759 g          277 t
ORIGIN      
        1 tcgacgacct gatcgtctac cgggaggccg acggttacct gctggtcccc aacgcggcca
       61 actggcccaa ggtggccgac gccgtccgcg cgtcggaccc gggccggacc gaggtcaccc
      121 cccgacccga catcgccgtg ctcgccgtcc agggcccccg cgcccccgag ctcgtcggcg
      181 gcctgttccc ggaggcggcc gacctgccct acatgcacct cgtggccacc acctaccggg
      241 acaccacggc ccgggtctgc cgcaccggct acaccggcga gcgcgggttc gagctgctcg
      301 ttcccggtga ggtcgccccc gccctgtggg aggagctgtt cgagcggggc gcggccttcg
      361 aggtcgtccc ggtcgggctg ggcggccgcg acaccctgcg actggagatg ggctacgccc
      421 tgcatggcaa cgacatctcc accgacaccg acccgttcga ggcccggctg ggctgggccg
      481 tggccatgga caagggcgac ttccggggcc gcgaggccct ggccgcccgc aaggaggccg
      541 gccccaagcg gctgctggtc ggcctcgtcg ccgccgaccg gctgatcccg cgccacggca
      601 tggccgtgct cgacggcgac cgcccggtcg gcgaggtcac cagcggcacc ttctcgccga
      661 ccctgcgcca gggcatcgcc ctggcctacg tgctgcccga cgtcgccgcc aagggcaccg
      721 agctccaggt cgacgtccgc ggcaagcgcg gccaggtgac ggtgacccgg ccgccgttcg
      781 tcgggtcctc acccaggtag gcatggcgac cggggtgggc tccgagctgg tcgtgctcct
      841 cgacgaggac ggcaggccgg ccgggaccat gcccaaggcc cgggtccacg ggccggacac
      901 gccgctgcac ctggccttct cggtctacct gttcgacgcc ggggggcgct tcctggccac
      961 ccgccgggcg ctcggcaagc ggacctggcc gggggtgtgg accaactcct gctgcggcca
     1021 cccggccccc ggggaggagc cggccgacgc cgcccggcgc cgcctgggcc aggagctcgg
     1081 cctccacccg gcccgcatgg agctggtcct gccctcgttc gcctaccggg cggtggccgc
     1141 cgacggggtg gtcgagaacg aggtctgccc ggtgttcttc gcccacctgg acggcgaccc
     1201 ggagccgccg ccgacccccg accccgagga ggtggccgag tggcgctggg tgccctgggc
     1261 ggcgttccgc gccgtcgcca ccacggcccc gtgggccctc agcccctggt cggtcgagca
     1321 ggtccgtcag ctccccgcct gatcgaccgc aaccccggcg gcttcggtcg cgtgtagccg
     1381 acatggcccg acacgacccg gacttcctgg agttcttcgc cgccgagttc tggcccctgc
     1441 ggcgggtcgg gttcctgctc accggcgact gggaccaggc cgaggagctg gcccaggagg
     1501 cgatggcccg cacctacgcg gtgtggcccc gggtccgggg gtatgaccgc ccggccgcct
     1561 acgcccgcaa ggtgctgctc aaccggcacc gctcgctgct ccgccgggcc gtggtcgagg
     1621 cccgccacct gctcaccagc caccccgacg accgccacca gcccgacttc ggcggcgacg
     1681 acctggtcct gtggcaggcc ctccaggggc tgccgtcccg ccagcgggcc gcgctcgtgc
     1741 tccgcttcta cctcgacctg cccgaggccg aggtcgcccg cctcctgggc gtcccggcgg
     1801 gcacggtcaa gtcctggacc caccgcggcc tggcccgcct gcgcgaccgc ctcggtccca
     1861 cctacgccga cgagcgcctc gccggcaacc ccagcacgga ggccggcaac ttccacttcc
     1921 ggtacccgcc gggctgggag gcgcgccgga tcgggggcca gtgggccctc accctgcggg
     1981 gcatccctcg tagcgaccag ggggtgccgg agttcgaggt gagggtcacg tccgctcgga
     2041 gcctgtggag gtcccacgga tactggcagg gcaccgcccc ggaggtcggg cgactgcccg
     2101 ccgggcaggc ctacctgctc aagtcccacg ccccggcgga gtacagcacc t
//
LOCUS       DASSWR010000057         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_934522,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000057 DASSWR010000000
VERSION     DASSWR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934522"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..812)
                     /locus_tag="VFO47_00975"
     CDS             complement(162..812)
                     /locus_tag="VFO47_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HET8787302.1"
                     /translation="MSDTTPTPERSSKPVVTLFESYGSGASYIGPRVAQALDVPFHPQ
                     AFSSEEIENAMVERESEGLLSQVFSAMGGQYVGIEGGSVAMAQQDKHDLVMENTRKVV
                     EDARQGGVIVGRNGALILANWPTALHVKLDGPLTQRIERAAKDSGIDIERAAKRQKRE
                     DQVRADMSIEFYGWDPRETERYDLMVNTGTMDLDTCVDIIVQAALVKAGLASAASP"
     gene            complement(837..2240)
                     /locus_tag="VFO47_00980"
     CDS             complement(837..2240)
                     /locus_tag="VFO47_00980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HET8787303.1"
                     /translation="MSETRTTAGTLSRVGGHGRPAAPVRLVHLGLGNFFRAHQAWYTD
                     RAPDAGEWGIAAFTGRSAEPAEALTTQQGLYTLITRAADEDRFEVLASLSRAHPGTDH
                     QSWLGRLASPEVRVVTITVTEAGYLLGADGGLDRDRPQVQADVEALRHDRSALVRTAP
                     ARLVAGCAARRRAEAGPLTLVPCDNLPDNGAVVARVVRDLAEMVDPGLAGWLAGSIAF
                     ATTMVDRITPKTTQEDLHTVKAATGLDDRAPVATEPFSEWVLSGPFPGGRPRWEDAGA
                     TFTDDIQPYEQRKLWLLNGGHSLLAYAGSARGHQTVAEAVADETCRAWLEEWWSEASR
                     HLSLPAADVAAYRAALLDRFANPRMHHRLDQIAADGSQKLPVRILPALRRERAAGRLP
                     EGAARALAAWVCHLRGVGAAVTDARADQVVPLAAGPLPEAVPRGLAALDPALADDGDL
                     VAAVLAHADQLGQPKRS"
     gene            complement(2237..>2484)
                     /locus_tag="VFO47_00985"
     CDS             complement(2237..>2484)
                     /locus_tag="VFO47_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET8787304.1"
                     /translation="APDATRRFRAAVTETVGFSRTSGFVDDTRAFCSIPARHDMSRRI
                     DAGWLAQLVAEHRLDEDEAAETALDLVTGRPREVFKL"
BASE COUNT          323 a          866 c          932 g          363 t
ORIGIN      
        1 aggcgctcat agccgatgag gggctcgtga tcgtgagcaa ggaggaggcg acgagtcggg
       61 ccaccagggt gcccgcgaag ttggggttga acgccttcac gaccggtgtc cctgcgggac
      121 gcgctgctgg acctcctcgc aggcgttgag cggcagggcg gttacgggga tgcggcgctc
      181 gcgagcccag ccttgaccag tgcggcctgg acgatgatgt cgacgcaggt gtccaggtcc
      241 atggtccccg tgttgaccat caggtcgtag cgctccgtct cccgggggtc ccaaccgtag
      301 aactcgatcg acatgtcggc gcggacctgg tcctcccgct tctgacgctt cgcggcccgt
      361 tcgatgtcga ttccgctgtc cttcgcggct cgttcgatcc gctgcgtgag gggaccgtcc
      421 agcttgacgt ggagcgccgt cggccagttc gccaggatca gggccccgtt gcgtccaacg
      481 atcaccccgc cctgacgcgc gtcctccacc accttgcggg tgttctccat gacgaggtca
      541 tgcttgtcct gctgggccat ggccaccgat ccgccctcga tcccgacgta ctggccgccc
      601 atggcgctga agacctggga gagcagccct tcgctctcac gctcgaccat ggcattctcg
      661 atctcctcgg acgagaacgc ctgtgggtgg aagggcacgt ccagcgcctg tgccacccgg
      721 gggccgatgt agctcgcgcc cgacccgtac gactcgaaca gggtcaccac gggcttggac
      781 gaacgctccg gggtgggtgt ggtgtcggac atcacaacct ccttgacgtc accgttctat
      841 gaccgcttcg gctgcccgag ctggtcggcg tgggcgagta cggccgccac caggtcgccg
      901 tcgtcggcga gggccgggtc gagggccgcc aacccgcgcg gcaccgcctc ggggaggggg
      961 ccggcggcga gcgggaccac ctggtcggcg cgggcgtcgg tgaccgccgc gccgacaccc
     1021 cgcaggtggc acacccaggc ggcgagggcg cgggccgcgc cctcgggcag ccgaccggcg
     1081 gcccgctccc gccgcagggc cggcaggatg cggaccggca gcttctggga gccgtcggcg
     1141 gcgatctggt ccagccggtg gtgcatcctt gggttggcga accggtccag gagagcggcg
     1201 cggtaggccg cgacgtcggc tgccggcagg ctcaggtgcc gggacgcctc cgaccaccac
     1261 tcctccagcc aggcccggca ggtctcgtcg gccaccgcct cggccaccgt ctggtgtccc
     1321 cgggcggatc cggcgtaggc cagcaacgag tgaccgccgt tgagcagcca gagcttgcgc
     1381 tgctcgtaag gttggatgtc gtcggtgaag gtggcgcccg cgtcctccca gcgcggccgg
     1441 ccgccgggga acgggccgct gagcacccac tcgctgaacg gctcggtggc gaccggggcg
     1501 cggtcgtcca ggccggtggc cgccttgacc gtgtggaggt cctcctgggt ggttttcggc
     1561 gtgatccggt cgaccatggt ggtggcgaag gcgatcgacc cggcgagcca gccggcgagg
     1621 ccgggatcga ccatctcggc caggtcgcgg accacccggg ccaccacggc accgttgtcc
     1681 ggcaggttgt cgcacgggac cagggtgagt ggccccgcct cggcgcgccg ccgggcggcg
     1741 cagccggcga ccagccgggc cggggcggtg cgcaccaggg cactgcggtc gtgacggagc
     1801 gcctcgacgt cggcctggac ctggggccga tcgcggtcca gcccaccgtc ggcgccaagg
     1861 aggtagccag cttcggtcac ggtgatggtg accacgcgca cctccggcga ggcgagccgg
     1921 cccagccaac tctgatggtc ggtccctgga tgcgcccgcg acaggctggc cagtacctcg
     1981 aagcggtctt cgtcggccgc ccgggtgatc agcgtgtaga ggccttgttg ggtggtgagg
     2041 gcctcggccg gctcggccga gcggccggtg aaggcggcga tgccccactc gccggcgtcg
     2101 ggggcccggt cggtgtacca ggcctggtgg gcgcggaaga agttgccgag cccgaggtgc
     2161 accaggcgga ccggcgccgc gggacggccg tgcccgccga cgcgggacag cgtcccggcg
     2221 gtggtccggg tctcgctcac agcttgaaca cctcccgagg ccgcccggtg accaggtcga
     2281 gcgccgtctc ggcggcctcg tcctcgtcga gccggtgctc ggccaccagc tgcgccagcc
     2341 agcccgcgtc gatgcggcgg gacatgtcgt ggcgggccgg gatcgagcag aacgcccggg
     2401 tgtcgtcgac gaacccggag gtccgcgaga agcccacggt ctcggtgacc gcggctcgga
     2461 accgccgggt cgcgtcggga gcgt
//
LOCUS       DASSWR010000058         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_143489,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000058 DASSWR010000000
VERSION     DASSWR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143489"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFO47_00990"
     CDS             <1..1154
                     /locus_tag="VFO47_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787305.1"
                     /translation="RAGRGRNPVVGKRSGGRKNGQATPLTMRGIGLLAGGLLLGVGAG
                     VTLERTLVGRDRRREDPEAGERFGGIRGTSAKVASHDGTRIHVEELGQGPCLVFAHGF
                     SLTQDAWHYQRRDLPDRFRCVFYDQRGHGRSGRPGKEDYSLQALAGDLKAVLDWTGEE
                     RVVVVAHSMGGIAALQLAEQFPQELGGRVAGLVLVGSTYVDTVRGMTAAVSAWGSAWA
                     QRTLITGAFRFMGQDPVRAQQLRRRGSDLGYLGTRLFGFGSNPSPSQVAFIDRTLAGT
                     DVEVWAKVFPSLVDFDLSESLEAVGVPALVVVGDKDRLTPPAAARYMAEKIPGSRLLI
                     LEDAGHCAFLEEHEVLDAEIVAFADEVLVPAKAAARKRRRTEVRRVQGS"
     gene            1162..2139
                     /locus_tag="VFO47_00995"
     CDS             1162..2139
                     /locus_tag="VFO47_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET8787306.1"
                     /translation="MALGIEDLAVGHWTDPVGLTGCTVVVPPPGNVAAASVRGGGPGT
                     RETDLLQPQAHVEGVSAVLLTGGSAFGLHAAQGVVDWCERRGLGYGRFGRPIPIVPAA
                     VLFDLMVGDWDARPGPAEGEAACLAASTADGPLGNVGAGMGATVGKTAGPEHMTKGGL
                     GWSVLEAGPVTVGALAAVNAGGDVLDEDGTVLAGARVPGGARTALRERLASPTADGEV
                     RPEPVPPGGSTTLALVATNAILTKGEVHRVAVQAHDGMARAIAPVHTSFDGDTVFAVA
                     VPRVQATMDLVAFLAEEALAAAIRAGVRAAGPVAGIPAASDASSGAGGE"
     gene            2141..>2293
                     /locus_tag="VFO47_01000"
     CDS             2141..>2293
                     /locus_tag="VFO47_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET8787307.1"
                     /translation="MPLYEYHCADCGHQFEALVPAGRADDETCPDCGTQARRLLSLVA
                     APVRGSD"
BASE COUNT          269 a          844 c          903 g          277 t
ORIGIN      
        1 aacgggctgg gcgggggcgg aaccccgtcg tcggcaagcg ttccgggggg cggaagaacg
       61 ggcaggcgac gccgctgacc atgcggggga tcgggctgct ggccgggggg ctgctgctcg
      121 gggtcggggc gggggtgacc ctggagcgga cgctggtggg acgggaccgg cggcgcgagg
      181 accccgaggc cggggagcgg ttcgggggga tccgggggac ctcggccaag gtggccagcc
      241 acgacggcac ccggatccac gtcgaggagc tgggccaggg cccctgcctg gtgttcgccc
      301 acggcttctc actcacccag gacgcctggc actaccagcg ccgcgacctg cccgaccggt
      361 tccgctgcgt cttctacgac cagcgcggcc acggccgctc gggccggccc ggcaaggagg
      421 actactcgct ccaggccctg gccggcgacc tcaaggcggt gctcgactgg accggggagg
      481 agcgggtggt ggtggtcgcc cactcgatgg gcggcatcgc cgccctccag ctcgccgagc
      541 agttccccca ggagctgggc ggccgggtcg ccgggctggt cctggtcggc tccacctacg
      601 tcgacaccgt ccggggcatg accgccgcgg tcagcgcctg ggggtcggcg tgggcccagc
      661 ggaccctgat caccggcgcc ttccgcttca tgggccagga cccggtccgg gcccagcagc
      721 tccgccggcg cggcagtgac ctcggctacc tgggcacgcg cctgttcggc ttcggctcga
      781 acccctcgcc cagccaggtc gccttcatcg accggaccct ggccggcacc gacgtcgagg
      841 tctgggccaa ggtcttcccc agcctggtcg acttcgacct gtcggagtcg ctggaggcgg
      901 tcggcgtgcc cgccctggtc gtggtggggg acaaggaccg cctcaccccg ccggcggcgg
      961 cccggtacat ggccgagaag atccccgggt cgcgcctgct gatcctggag gacgccggcc
     1021 actgcgcctt cctggaggag cacgaggtgc tcgacgccga gatcgtcgcc ttcgccgatg
     1081 aggtgctggt cccggccaag gcggccgccc ggaagcgccg gcgcacagaa gtccgccggg
     1141 tccaggggtc ctagaccggc cgtggcactc ggcatcgagg acctggccgt cggccactgg
     1201 accgacccgg tcgggctgac cggctgcacg gtggtggtgc cgccgccggg gaacgtggcc
     1261 gcggccagcg tccgcggtgg cgggcccggg acccgcgaga ccgacctgct ccagccccag
     1321 gcccacgtcg agggcgtgtc ggcggtgctg ctcacgggcg ggagcgcgtt cgggctgcac
     1381 gccgcccagg gggtggtcga ctggtgcgag cggcgcgggc tcggctacgg ccgcttcggc
     1441 cggcccatcc cgatcgtgcc cgcggccgtg ctgttcgacc tgatggtcgg ggactgggac
     1501 gcccggcccg gcccggccga gggcgaggcc gcctgcctgg ccgcctcgac cgccgacggg
     1561 ccgctcggca acgtcggcgc cggcatgggc gccaccgtcg gcaagacggc cgggcccgag
     1621 cacatgacca agggcgggct cggctggtcg gtgctcgagg ccgggccggt cacggtcggc
     1681 gccctggccg ccgtcaacgc cggcggcgac gtgctcgacg aggacgggac cgtgctcgcc
     1741 ggggcccggg tgcccggcgg ggccaggacc gccctgcgcg agcggctggc ctcgccgacg
     1801 gccgacgggg aggtccggcc cgagccggtc ccgcccggcg gcagcaccac cctggccctg
     1861 gtcgcgacca acgccatcct gaccaagggc gaggtccacc gggtcgccgt gcaggcccac
     1921 gacggcatgg cccgggcgat cgcgcccgtc cacaccagct tcgacggcga caccgtgttc
     1981 gcggtggccg tgcccagggt gcaggcgacc atggacctgg tagccttctt ggccgaggag
     2041 gcgctggctg cggccatccg ggccggggtc cgggccgccg gcccggtcgc cggcatcccg
     2101 gccgcctccg acgcatcgag cggagcagga ggggagtagc gtgccgctct acgagtacca
     2161 ctgcgcggac tgcgggcacc agttcgaggc cctggtcccg gccgggcggg ccgacgacga
     2221 gacctgcccg gactgcggga cccaggcccg ccggctgctg agcctggtcg ccgccccggt
     2281 ccgggggagt gac
//
LOCUS       DASSWR010000059         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_510737,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000059 DASSWR010000000
VERSION     DASSWR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510737"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2404
                     /locus_tag="VFO47_01005"
     CDS             <1..>2404
                     /locus_tag="VFO47_01005"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HET8787308.1"
                     /translation="LFPPAAPTPFGRLLSAAFDRVPGAIAFRTTNKAGALVALAYALL
                     LALGAAELFRRSAKGERPWRGAAAAGVAVILAVSVLPAWTGGLYPIRYQVPGYWHQLA
                     ADVNAGSADTRVLLAPGASNTAYRWGMEGPDDVNLSLLSRPSVVRTTVPNGSFEQTNF
                     LAALDLPLSTGDADPEVVAAMARYLGVAEVVVRNDQRWEAFGGPRPSVVAERLAGDPS
                     LDLVRSYGRPGEHTVAPVSDFYPPDQALADADLPPLQRYAVARPRPIVRTEPTRGAML
                     VDGDNFAIGSLVRLGLAKGEPAFRLLGSVTPAELAGSLAGGARVVVSDSNRRRAWGSF
                     RTGQNYSPTLRADQPLPTSTLTLFGDPDTQTVTRLEGAASVTATGSGSLLTATPFGKP
                     EHAFDGDRRTAWTTGDFGGAVGQSVTVRLSEPVEVGSIMLRPVLSEPGVEVAGVRVRV
                     DAGAVEVPVPAEPEVRVDVPDATTTSVTVEITQVRGVGLNPVGFWEIGVPGVEVRQVA
                     RLPRTLRRLAGALPEADRARLDAAPIDVVLTRAAGEAGDRFHDEERVLDRELWLPAAR
                     DFTVGGLAAAGPGLPDPVVDRLAGASGRVVASSSSRFFDEVGARASQALDGDPDSAWV
                     PDGRGPGEWIELRLPGERLDHVDVLQSVPKGLVGVDAITAAEVSVNGGKPFLVNLRQG
                     RSRVDFPQTRVRRLRLTIDRVAGLGGQVRISELTAGRARIPAAPSSRRLRGCVELARV
                     DGDPLQVRLAGTVGDLAAGEALPVRGCDGPLRLGAGDHRVRSSPGWLVDLLGLSSPAP
                     ASR"
BASE COUNT          246 a          938 c          919 g          301 t
ORIGIN      
        1 ctgttcccgc cggccgcccc gacgccgttc gggcggctgc tgtcggccgc cttcgaccgg
       61 gtcccggggg cgatcgcctt ccgcaccacc aacaaggcgg gagcgctggt cgcgctggcg
      121 tacgcgctgc tgctcgccct gggggcggcc gagctgttcc gccggagcgc caagggcgaa
      181 cgaccgtggc ggggggcggc ggccgccggc gtggccgtga tcctggccgt gtcggtgctg
      241 ccggcctgga cgggcgggct gtacccgatc cgctaccagg tccccggcta ctggcaccag
      301 ctggccgccg acgtcaacgc ggggtcggcc gacacccggg tgctgctcgc cccaggggcc
      361 agcaacaccg cctaccgctg gggcatggaa gggcccgacg acgtcaacct gtcgctgctg
      421 tcgcggccct cggtggtccg gaccaccgtg cccaacggct ccttcgagca gaccaacttc
      481 ctggccgccc tcgacctgcc gctgagcacc ggcgacgccg accccgaggt ggtcgcggcc
      541 atggcccgct acctgggcgt ggccgaggtg gtggtccgca acgaccagcg ctgggaggcg
      601 ttcggcgggc cgcggccctc ggtggtggcc gagcgcctgg ccggcgaccc ctcgctcgac
      661 ctggtccggt cctacggccg ccccggcgag cacacggtgg ccccggtctc cgacttctac
      721 ccgcccgacc aggcgctggc cgacgccgac ctgccgccgc tgcagcgcta cgccgtggcc
      781 cggccccggc cgatcgtccg gaccgagccg acccgggggg cgatgctggt cgacggcgac
      841 aacttcgcca tcgggtcgct ggtccgcctc gggctggcca agggcgagcc ggcgttccgg
      901 ctgctcggct ccgtcacccc ggccgagctg gccgggtcgc tggccggcgg agcccgggtg
      961 gtggtctccg acagcaaccg gcgccgggcc tggggcagct tccgcaccgg ccagaactac
     1021 tcgcccaccc tccgggccga ccagccgctg cccacctcga ccctgaccct gttcggcgac
     1081 ccggacaccc agaccgtcac ccggctggag ggggcggcgt cggtgaccgc caccgggtcg
     1141 ggctcgctgc tcaccgccac cccgttcggc aagccggagc acgccttcga cggcgaccgg
     1201 cggaccgcct ggacgaccgg cgacttcggc ggcgccgtcg gccagtcggt caccgtccgc
     1261 ctgtccgagc cggtcgaggt ggggtcgatc atgctccgcc cggtgctgtc cgagccgggc
     1321 gtggaggtcg ccggggtgcg ggtccgggtc gacgccggcg cggtcgaggt cccggtgccg
     1381 gccgagcccg aggtccgggt cgacgtgccg gacgccacca ccaccagcgt gaccgtggag
     1441 atcacccagg tccgcggggt cggcctcaac ccggtgggct tctgggagat cggcgtgccc
     1501 ggggtcgagg tccgccaggt cgcccgcctg ccccggaccc tgcgccggct ggccggcgcc
     1561 ctcccggagg ccgaccgggc ccggctcgac gccgccccga tcgacgtggt cctgaccagg
     1621 gcggccggcg aggccggcga ccgcttccac gacgaggagc gggtgctcga ccgcgagctg
     1681 tggctaccgg cggcgcggga cttcaccgtc ggcgggctgg ccgcggccgg gccggggctg
     1741 cccgacccgg tggtggaccg gctcgccggc gcctccggca gggtcgtggc cagctcctcc
     1801 tcccgcttct tcgacgaggt cggggcccgg gcctcccagg ccctggacgg cgacccggac
     1861 agcgcctggg tgccggacgg ccgcgggccc ggcgagtgga tcgagctgcg cctgcccggc
     1921 gagcggctcg accacgtcga cgtcctccag tccgtgccca aggggctggt cggggtggac
     1981 gccatcaccg ccgccgaggt ctcggtcaac ggcggcaagc cgttcctggt caacctgagg
     2041 cagggacggt cccgggtcga cttcccccag accagggtgc ggcggctgcg gctgaccatc
     2101 gacagggtgg ccgggctggg cggccaggtg cggatcagcg agctgacggc gggccgggcc
     2161 cgcatcccgg cggcgccgtc gtcgcggcgc ctgcggggct gcgtcgagct ggccagggtc
     2221 gacggcgacc cgctccaggt gcggctggcc ggcaccgtcg gtgacctggc cgccggcgag
     2281 gccctgcccg tccgcggctg cgacgggccc ctgcggctcg gcgccggcga ccaccgggtc
     2341 cggtccagcc ccggctggct ggtcgacctg ctggggctgt cgtcgccggc ccccgcctcg
     2401 cggc
//
LOCUS       DASSWR010000060         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_567479,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000060 DASSWR010000000
VERSION     DASSWR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567479"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFO47_01010"
     CDS             <1..138
                     /locus_tag="VFO47_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006067998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8787309.1"
                     /translation="DPSEEEIRWALSGNLCRCTGYQNIVNAVQWTARKLQSDSAATGA
                     S"
     gene            148..>2071
                     /locus_tag="VFO47_01015"
     CDS             148..>2071
                     /locus_tag="VFO47_01015"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HET8787310.1"
                     /translation="MTQDHTAEHWLGKSVKRKEDDRFLQGKGNYVDDINLPGMLHMSI
                     LRSPVAHARINSIDTSAAEAVDGVVAVVTGELLAQHNLAWMPTLSGDTQAVLATDKVR
                     MQGQEVACVIAEDPYIAHDALELIEVDYDPLPVVITPQQSLEPGAPVIRDDKEDKHDN
                     RIYHWEAGDKEATDEAFAKADRVVSYETFYPRCHPAPMETCGCVADVNPVTGKATIYM
                     TSQAPHAHRTVFALVAGLPEHQIQIISPDIGGGFGNKVPIYPGYVVATAASLLLGRPV
                     KWIEDRTGNLISTGFARDFYMKGELAVDNDGKMLGLRVNMLSDQGYAYADAQPSKLKA
                     GLFHIVTGSYDIPAAHVVTDGAYTNKAPGGVAYRCSFRVTEASFFIERLVQTAAYELG
                     IDPVELRRKNFIQPEQFPYTSATGFVYDSGNYEAALDKALEMLGYEDLKAEQARARDE
                     GKLIGIGLATFTEVVGAGPSKDYDILGLKMFDSAELRVHPTGKAILKIGVQTQGQGHE
                     TTFAQIVSNELGIPPEDILVQHGDTDNTPYGLGTYASRSTPTAGAATAMVSRKLREKA
                     KQLAAHLLEVAPEDLEWVGDRFQVQGAPDRQTTIQEAAFAAYTNHPQGMEAGLEGVTY
                     YDPPNMTYPFGAYAVAV"
BASE COUNT          378 a          782 c          652 g          259 t
ORIGIN      
        1 gaccccagcg aggaggagat ccgctgggcc ctgtccggca acctctgccg ctgcacgggc
       61 taccagaaca tcgtcaacgc ggtgcagtgg acggcgagga agctccagtc cgacagcgcc
      121 gcgaccggcg ccagctagga ggccgacatg acccaggatc acacggccga gcactggctg
      181 ggcaagagcg tcaagcgcaa ggaggacgac cgcttcctcc agggcaaggg caactacgtc
      241 gacgacatca acctgcccgg catgctgcac atgtcgatcc tgcgcagccc ggtggcccac
      301 gcccgcatca actcgatcga cacctcggcc gccgaggccg tcgacggcgt ggtcgcggtg
      361 gtcaccggcg agctgctggc ccagcacaac ctggcctgga tgccgaccct gtccggggac
      421 acccaggccg tgctggccac cgacaaggtg cgcatgcagg gccaggaggt cgcctgcgtg
      481 atcgccgagg acccctacat cgcccacgac gccctcgagc tgatcgaggt cgactacgac
      541 ccgctgccag tggtcatcac cccccagcag agcctcgagc cgggcgcccc ggtgatccgg
      601 gacgacaagg aagacaagca cgacaaccgc atctaccact gggaggcggg cgacaaggag
      661 gccaccgacg aggccttcgc caaggccgac cgggtggtct cctacgagac cttctacccg
      721 cgctgccacc cggccccgat ggagacctgc ggctgcgtgg ccgacgtcaa cccggtcacc
      781 ggcaaggcca ccatctacat gacctcccag gccccgcacg cccaccggac ggtgttcgcg
      841 ctggtcgccg gcctgcccga gcaccagatc cagatcatct cgcccgacat cggcggtggc
      901 ttcggcaaca aggtccccat ctaccccggc tacgtggtcg ccaccgcggc ctcgctgctg
      961 ctgggccggc cggtcaagtg gatcgaggac cgcaccggca acctgatctc caccggcttc
     1021 gcccgcgact tctacatgaa gggcgagctc gccgtggaca acgacggcaa gatgctcggc
     1081 ctgcgggtca acatgctgtc cgaccagggc tacgcctacg ccgacgccca gccgagcaag
     1141 ctcaaggcgg gcctgttcca catcgtcacc ggctcctacg acatccccgc ggcccacgtg
     1201 gtcaccgacg gcgcctacac caacaaggcc cccggcgggg tcgcctaccg ctgctcgttc
     1261 cgggtcaccg aggcgtcgtt cttcatcgag cgcctcgtcc agacggccgc ctacgagctc
     1321 gggatcgacc cggtcgagct gcgccggaag aacttcatcc agcccgagca gttcccctac
     1381 acctcggcca ccggcttcgt ctacgactcc ggcaactacg aggcggcgct ggacaaggcc
     1441 ctggagatgc tcggctacga ggacctcaag gccgagcagg cccgggcccg cgacgagggc
     1501 aagctgatcg ggatcgggct ggccaccttc accgaggtgg tcggggccgg gccgagcaag
     1561 gactacgaca tcctcggcct gaagatgttc gactcggccg agctgcgggt ccacccgacc
     1621 ggcaaggcga tcctcaagat cggcgtgcag acccagggcc agggccacga gacgaccttc
     1681 gcccagatcg tgtccaacga gctcggcatc ccgcccgagg acatcctggt ccagcacggc
     1741 gacaccgaca acacccccta cgggctcggc acctacgcct cccggagcac cccgacggcc
     1801 ggggcggcca cggccatggt ctcgcgcaaa ctgcgcgaga aggccaagca gctggccgcc
     1861 cacctgctgg aggtcgcccc cgaggacctg gagtgggtcg gcgaccgctt ccaggtgcag
     1921 ggcgccccgg accggcagac caccatccag gaggccgcct tcgccgccta caccaaccac
     1981 ccgcagggga tggaggccgg gctggaaggc gtcacctact acgacccgcc caacatgacc
     2041 tacccgttcg gggcctacgc ggtcgccgtc g
//
LOCUS       DASSWR010000061         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_765448,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000061 DASSWR010000000
VERSION     DASSWR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765448"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFO47_01020"
     CDS             complement(<1..996)
                     /locus_tag="VFO47_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET8787311.1"
                     /translation="MAETAEPTLVELRVLDGANLYFPRPAIKLTLEVPALLSLPEERA
                     AAVAGEVGLMAGAPGPPGGEQRRRFATRRRAHLVRRTAAAAGVRARAVRSRPGPEPAQ
                     VVVAYPWRRQHAAEALGRAVAGLTDGLDTGGLAERVEAAGAALRQVEPGPGPSLPAPR
                     IPTVAVTGTNGKTTVTRLVAHMGRCAGLHVGWSNTDGIYLDGELVDEGDWSGPGGAAR
                     VLAQPGIQLAVLETARGGILRRGVGMAHNDVAVVTNISADHLGLGGIHTLDQLAEVKA
                     TIVRITRAGGWVVLNADDPRTLAMRRLSRARPFVFGLDPDAPGLREAQTEGGRAAT"
     gene            1027..1983
                     /locus_tag="VFO47_01025"
     CDS             1027..1983
                     /locus_tag="VFO47_01025"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET8787312.1"
                     /translation="MIGSAGTTKVAVVGAGSVGATCAYACLIQGIGRTIALYDINAAK
                     TRAEVLDLNHGLQFVPQATVVGSDDVEVCRGADLVVITAGAKQKPGQTRLELAEANVN
                     LSKAIVPRLLEVAPEAILLLVTNPVDVITYAALKISGLPPQRVFGSGTVLDSSRLRLL
                     VADHCGVAVQNVHAYIAGEHGDSEVPLWSSASIGSVPLLAWDVPGRPPLDAAAREDIH
                     RRVVGAASEIIRGKGATNYAVGLAAARIIEAVLRDQRQVLPVSSLLDGQAGIDDVCLS
                     LPSVVDRLGVEMVLPSPMSAEEVAGLRRSAETVKGVIKSVGL"
BASE COUNT          242 a          792 c          762 g          255 t
ORIGIN      
        1 ggtggcggcg cgcccgccct cggtctgggc ctcgcgcagc ccgggcgcgt ccgggtcgag
       61 gccgaacacg aacgggcggg cccgcgacag ccggcgcatg gccagggtcc gcgggtcgtc
      121 ggcgttgagc accacccagc cgccggcgcg ggtgatccgg acgatcgtgg ccttgacctc
      181 ggccagctgg tcgagggtgt ggatgccgcc gaggccgagg tggtcggcgg agatgttggt
      241 gaccacggcc acgtcgttgt gggccatccc gaccccgcgg cgcaggatgc cgccccgggc
      301 cgtctcgagc acggccagct ggatgccggg ctgggccagg acccgggcgg ccccgcccgg
      361 ccccgaccag tccccctcgt cgaccagctc gccgtccagg tagatgccgt cggtgttgga
      421 ccagccgacg tgcagcccgg cgcaccggcc catgtgggcg acgagccgcg tcaccgtcgt
      481 cttgccgttg gtgccggtca ccgccaccgt cgggatcctg ggcgcgggca gcgaggggcc
      541 ggggccgggc tcgacctggc gcagggccgc ccccgccgcc tccacccgct cggccaggcc
      601 gccggtgtcg aggccgtcgg tcagcccggc caccgcccgt ccgagggcct cggcggcgtg
      661 ctggcggcgc cacgggtagg cgaccaccac ctgggccggc tcggggccgg ggcggctgcg
      721 cacggcccgc gcccgcaccc cggcggcggc cgcggtccgc cggaccaggt gggcccgccg
      781 ccgggtggcg aagcggcgcc gctgctcgcc cccgggcggg cccggcgccc cggccatcag
      841 gccgacctcg ccggccaccg ccgccgcccg ctcctcgggc agcgacagca gcgccggcac
      901 ctccagggtg agcttgatcg ccgggcgggg gaagtacagg ttggccccgt cgagcacgcg
      961 cagctcgacc agcgttggct ccgccgtctc ggccatggcc cggacattat ccggtagggt
     1021 tccctcatga tcggatcggc gggcaccacc aaggtggcgg tggtgggggc cggcagcgtc
     1081 ggggccacct gcgcctacgc ctgcctgatc caggggatcg ggcggaccat cgccctctac
     1141 gacatcaacg ccgccaagac ccgggccgag gtgctcgacc tcaaccacgg cctccagttc
     1201 gtcccccagg ccaccgtcgt cggctccgac gacgtcgagg tctgccgcgg ggccgacctg
     1261 gtcgtgatca ccgccggggc caagcagaag cccgggcaga cccgcctgga gctggccgag
     1321 gccaacgtca acctctccaa ggcgatcgtc ccgcggctgc tcgaggtcgc ccccgaggcg
     1381 atcctgctgc tggtcaccaa cccggtcgac gtcatcacct acgcggctct gaagatctcg
     1441 ggcctgccgc cgcagcgggt gttcgggtcg ggcacggtgc tggacagctc gcggctgcgg
     1501 ctgctggtcg ccgaccactg cggcgtggcc gtgcagaacg tccacgccta catcgccggc
     1561 gagcacgggg actccgaggt ccccctgtgg agcagcgcct ccatcgggtc ggtgccgctg
     1621 ctggcctggg acgtccccgg ccggcccccg ctggacgcgg ccgcccgcga ggacatccac
     1681 cgccgggtgg tcggcgccgc cagtgagatc atccgcggca agggcgccac caactacgcg
     1741 gtcgggctgg ccgcggcccg gatcatcgag gccgttctgc gcgaccagcg ccaggtgctg
     1801 cccgtcagct ccctgctcga cggccaggcc ggcatcgacg acgtctgcct gtcgctgccg
     1861 tcggtggtgg accgcctcgg cgtcgagatg gtcctgccca gcccgatgtc ggccgaggag
     1921 gtcgcgggcc tgcgccgctc ggccgagacg gtcaagggcg tcatcaagag cgtcggcctg
     1981 tgaccgaccc cagggccgag ccggcgagcc tgctggcgcc gctgcgcgaa cgcctcgacg
     2041 acgtcctggc c
//
LOCUS       DASSWR010000062         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_397802,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000062 DASSWR010000000
VERSION     DASSWR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397802"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFO47_01030"
     CDS             <1..1120
                     /locus_tag="VFO47_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET8787313.1"
                     /translation="TRGVNFAGFSSDGMRVVTTSQDATARYWDASAGQSSREFRGHSR
                     AVTNVAFSPDSHRLATAGGDKQVGIWNVTTGRLERFLTGHDREVSGAVFSPDGRRIAT
                     GSWDGTGAVRDAGSGQVLTRLKGHHREITAIAFSPDGARVVTASRDRTARIWEAETGR
                     QLFALSGHEAAVKSVAWSPDGRLIATASNDTTAKVWDAATGRLRAELVGHQEPLWSIG
                     FSPDGASVLTASEDRTARIWDFANAKVTTELRGHTGKVYGAAFHPDGTRVITSSEDRT
                     AYIWDTDTGRPLLELRGHAGAVNAAAFSPDGQLVATGGDDGAVRVYRITVRASVEDLL
                     VRARERVTRELTPEERVKYLREGDGAADVAQAPEIISL"
     gene            1117..1923
                     /locus_tag="VFO47_01035"
     CDS             1117..1923
                     /locus_tag="VFO47_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787314.1"
                     /translation="MTTWRARRRRVLDWLADDEHGLPALEAYAIAALAFGAIVFGIVT
                     DLPPGLYQAATLACLGFLVLSTVRQRAAQGFTIADVLRDRDSYEGFARLLDGTSELCF
                     YAPSGVNVLPQHLPDVKRWVQGGGRARFVVHDPEAAAAGGVRTQLDTNTDFLQDLERS
                     LTALARHAEPGKLDYRLLPLNPGFSIVVVNPGQRSGRVILELHGFQDPKVGDRMHLEI
                     RRTESVHWFEYWAERFEAIWLAAREPGADGRVTLSRVPADRTDDPEARPQ"
     gene            1920..2459
                     /locus_tag="VFO47_01040"
     CDS             1920..2459
                     /locus_tag="VFO47_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787315.1"
                     /translation="MTDRQRDDAVPSARLLVAYVSEDAELDHVRDAALEIGRRGAKVI
                     LYDRDSASAFSDPMPNQWASQAEGAQFNDPLSDQELVKLGREPFARKVAAARQAGVDA
                     WGWLASDHGTDALVAYARDHGADLILLPADLEDPGLSERLKGETVDKAVEEAEDTPSG
                     LAVVLVAPDGATELAAGRL"
     gene            2504..2695
                     /locus_tag="VFO47_01045"
     CDS             2504..2695
                     /locus_tag="VFO47_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787316.1"
                     /translation="MFRDEPTNELPREGAAAMYGPLIVAAAILLSIAGIVLFLHDLAD
                     ARARKAFDRIFEEGSRRRR"
     gene            complement(2913..3302)
                     /locus_tag="VFO47_01050"
     CDS             complement(2913..3302)
                     /locus_tag="VFO47_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET8787317.1"
                     /translation="MLTEVVVELEDRIGALARLGELLGEQGVNIRALAVVRVAEGRAL
                     AHLVVEPADVAVRVLREHDLVPERVREVLSVTLEDEPGALGRYCRRLADASINLEAVY
                     LAGERNGAKELVLAVSDLPAARQLRAR"
     gene            complement(3367..>3970)
                     /locus_tag="VFO47_01055"
     CDS             complement(3367..>3970)
                     /locus_tag="VFO47_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET8787318.1"
                     /translation="SACAMVEAPRFRLRSGRLLILAVLVVVAVVLPAGAALLDRRRPA
                     AQWESRTAPTVPPSVKVYEPGTPAPPLRLEGLDGRPVDLAALRGRPVVVNFWATWCEP
                     CVREFPLLAKAAATHRADRLAVVGVLTGDRPSEARAFVRRHNATWPVGIDADGAAAAA
                     WSAVGLPHTYFIRPDGTLASHHFSELTQPALDGQLAGILG"
BASE COUNT          554 a         1507 c         1456 g          453 t
ORIGIN      
        1 cacccgcggg gtcaacttcg ccggcttcag ctcggacggg atgcgggtcg tgaccaccag
       61 ccaggacgcg accgcgcgct actgggacgc cagcgccggg cagtcgtcgc gcgagttccg
      121 cggccacagc cgggccgtga ccaacgtcgc cttcagcccg gacagccacc ggctggccac
      181 cgccggcggc gacaagcagg tggggatctg gaacgtgacc accggccgcc tggagcggtt
      241 cctcaccggc cacgaccgcg aggtcagcgg ggccgtgttc agccccgacg gccgccgcat
      301 cgccaccggg agctgggacg ggaccggcgc cgtccgcgac gccggcagcg gtcaggtcct
      361 gacccggctc aaaggccacc accgcgagat cacggcgatc gccttcagcc ccgacggcgc
      421 cagggtggtg acggccagcc gcgaccggac ggcgcggatc tgggaggccg agacggggcg
      481 gcagctgttc gccctctccg ggcacgaggc cgcggtcaag agcgtcgcct ggagccccga
      541 cggccgcctc atcgccaccg ccagcaacga caccacggcc aaggtgtggg acgcggccac
      601 cggccggctc cgggccgagc tggtcgggca ccaggagccg ctgtggtcga tcgggttcag
      661 ccccgacggg gcctcggtgc tcacggccag cgaggaccgc acggcccgga tctgggactt
      721 cgccaacgcc aaggtgacca ccgagctgcg cggccacacc ggcaaggtct acggggccgc
      781 cttccacccc gacggcaccc gggtgatcac ctccagcgag gaccggacgg cctacatctg
      841 ggacaccgac accggccggc cgctgctgga gctgcgcggc cacgccgggg cggtgaacgc
      901 ggccgccttc agccccgacg gccagctggt ggcgaccggc ggtgacgacg gcgccgtccg
      961 cgtctaccgc atcaccgtgc gcgcgtccgt ggaggacctg ctggtgcggg cccgggagcg
     1021 cgtgaccagg gagctcacgc ccgaggagcg ggtaaagtac ctgcgagagg gggacggagc
     1081 cgccgacgtc gcccaggccc cggagatcat ctcgctatga ccacctggag ggcccgccgt
     1141 cgcagggtgc tcgactggct ggccgacgac gagcacgggc tcccggccct tgaggcgtac
     1201 gccatcgccg ccctggcttt cggggccatc gtcttcggca tcgtcaccga cctgcccccc
     1261 gggctgtacc aggcggccac cctggcctgt ctcggcttcc tggtcctgtc gaccgtccgg
     1321 cagcgggccg cccaggggtt cacgatcgcc gacgtgctgc gcgaccggga cagctatgag
     1381 ggcttcgccc gcctcctcga cgggacctcg gagctgtgct tctacgcccc cagcggggtc
     1441 aacgtgctgc cccagcacct gcccgacgtc aagcgctggg tccagggcgg cggccgggcc
     1501 cggttcgtcg tccacgaccc ggaggcggcc gcagccggcg gcgtccgcac ccagctcgac
     1561 accaacaccg acttcctcca ggacctggag cggtccctga ccgccctggc ccggcacgcc
     1621 gagcccggca agctcgacta ccggctgctg cccctcaacc ccggcttcag catcgtggtc
     1681 gtcaacccgg gccagcgcag cggccgggtc atcctcgagc tccacggctt ccaggacccc
     1741 aaggtcggcg accggatgca cctcgagatc cggcgcaccg agtcggtcca ctggttcgag
     1801 tactgggcgg agcggttcga ggccatctgg ctcgccgcgc gcgagccggg agcagacggt
     1861 agggtgaccc tgagccgcgt gcccgcggac cgcaccgacg acccggaggc cagaccccag
     1921 tgaccgaccg tcagcgcgac gacgccgtgc ccagcgcccg cctgctggtg gcctacgtct
     1981 ccgaggacgc cgagctcgac cacgtccgcg acgccgccct ggagatcggc cgccgcgggg
     2041 ccaaggtgat cctctacgac cgcgactcgg cctcggcctt cagcgacccc atgcccaacc
     2101 agtgggcctc ccaggccgag ggcgcccagt tcaacgaccc cctctccgac caggagctgg
     2161 tcaagctggg ccgggagccg ttcgcccgca aggtggccgc cgcccgccag gccggcgtcg
     2221 acgcctgggg ctggctcgcc tccgaccacg gcaccgacgc cctggtcgcc tacgcccgcg
     2281 accacggcgc cgacctgatc ctcctccccg ccgacctgga ggaccccggc ctcagcgagc
     2341 gcctcaaggg cgagaccgtc gacaaggccg tcgaggaggc ggaggacacc ccctccggcc
     2401 tggccgtcgt cctggtcgcc cccgacggcg ccaccgagct ggccgcgggt cgcctgtagc
     2461 gcttgcggcg gccgcgtagt tgacgccgct tgcggcatgt gcaatgttcc gcgatgagcc
     2521 cacaaacgag ctgccgcgag agggagccgc cgccatgtat gggccattga tcgtcgccgc
     2581 cgcgatcctg ctgtcgatcg ccggcatcgt cctgttcctg cacgacctcg ccgacgcgcg
     2641 tgcgcgcaag gccttcgacc gcatcttcga ggagggcagc aggcggcgcc gctgacgcgc
     2701 cccgggttca ccgcggccgg tcgcaccgtc gcggccagcc gcaagacgcc gacccggggc
     2761 gcggagccgc cgccaagcga gccaccgacg ccccgggtcg gcccaccgca ttatgacccg
     2821 ggcacccccc acggggcgaa ccccccggcc cggtcagatg cgccgcggcc cgccggccgc
     2881 ggccggccgc ggtgaacccg ggacgccgca ggtcaccggg cgcggagctg acgggcggcc
     2941 ggcaggtcgc tgacggccag gaccagctcc ttggcgccgt tgcgctcgcc ggcgaggtag
     3001 acggcctcca ggttgatgga ggcgtcggcg agccggcggc agtagcggcc gagggcgccg
     3061 ggctcgtcct cgagggtcac cgacagcacc tcgcgcaccc gctcgggcac caggtcgtgc
     3121 tcgcgcagga cgcgcacggc gacgtcggcg ggctcgacca ccaggtgggc cagggcccgc
     3181 ccctcggcca cccgcaccac ggccagcgcg cggatgttca ccccctgctc gccgagcagt
     3241 tccccgaggc gggccagcgc tccgatccgg tcctcgagct ccaccaccac ctcggtcagc
     3301 atcggcatgc ctcctcgtcg ggtgcaccgg cgcagcgttg tcggacggca tcgtctcacg
     3361 atggcgtcag ccgaggatcc cggccagctg gccgtcgagg gcgggttggg tgagctcgct
     3421 gaagtggtgg gaggcgaggg tgccgtcggg gcggatgaag taggtgtggg ggagaccgac
     3481 ggcactccag gcggcggcgg cggcgccgtc ggcgtcgatg ccgaccggcc aggtggcgtt
     3541 gtggcggcgg acgaaggcgc gggcttcgga ggggcggtcg ccggtgagga cgccgaccac
     3601 ggccaggcgg tcggcgcggt gggtggcggc ggccttggcg aggaggggga actcacggac
     3661 gcagggctcg caccaggtgg cccagaagtt gaccaccacc gggcggccgc ggagggcggc
     3721 caggtcgacc gggcggccgt cgaggccttc caggcgcagg ggcggggccg gggtgccggg
     3781 ctcgtagacc ttgaccgacg gggggacggt cggggcggtg cgggactccc actgggcggc
     3841 cggccggcgg cggtcgagca gggccgcccc cgccggcagg acgaccgcga ccacgaccag
     3901 gacggccagg atcagcagcc ggccggaacg gagccggaac ctaggagcct cgaccatggc
     3961 acaggcgctc
//
LOCUS       DASSWR010000063         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_652405,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000063 DASSWR010000000
VERSION     DASSWR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652405"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFO47_01060"
     CDS             complement(<1..197)
                     /locus_tag="VFO47_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787319.1"
                     /translation="MRQAGRPAFAHAASEAAHGITGRAARDAIRGAARGDVRRAARGD
                     ACAATRGVARAEQTRSRAGSPG"
     gene            207..422
                     /locus_tag="VFO47_01065"
     CDS             207..422
                     /locus_tag="VFO47_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HET8787320.1"
                     /translation="MSPLPGVVRRVEVRAGDRVEAGAVLVVVEAMKTEHRIAAPRAGR
                     VGRVLVSEGQEVAAGAAVAELEEPGDG"
     gene            415..1176
                     /locus_tag="VFO47_01070"
     CDS             415..1176
                     /locus_tag="VFO47_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8787321.1"
                     /translation="MAEAVSYELQDGVVWLVIERPEARNAINRAVADGLWEGFRRFEA
                     DPDAAVLVLTGAGDAFCAGADLKEMAALGLTVPPRDMTPNLGRNIEVTKPVVAAVNGP
                     AFGGGFLLAQMCDLCVAGTSARFAITEARWGRGAPWAAPLPWLVPPRVALELLLTGEP
                     IDARRAYEVGLVNRVVPDPELRSEAGRLARRIADNAPLSVRAAKAMVHATAGRALDEA
                     VEEGWRLFEPVYLSEDAQEGPRAFVEKRPPVWKGR"
     gene            1180..>2645
                     /locus_tag="VFO47_01075"
     CDS             1180..>2645
                     /locus_tag="VFO47_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HET8787322.1"
                     /translation="MEPLRIANCSGFYGDRRSAPRELLEGGPVDVLTGDYLAELTMLI
                     LWRSRSRDPAKGYATSFLAQMEECLGLAADKGVKVVANAGGLNPAGLAAELRALAGRL
                     GLDVVVAHVEGDDLLGRLDDLRAAGEPLAHLDSGVPLAAAGVVPVTANAYLGAWGIVA
                     ALEAGADVVVSGRVADASLVAGPAAWHFGWAADDWDRLAGAVVAGHVLECGTQATGGN
                     YAFLDELPGTRPPGFPIAEVAADGSAVVTKHPGTGGLVSAGTVTAQLLYEIAGPAYAS
                     PDVVARFDTVRIEEVGPDRVRLSGTRGEPAPDRLKVAVNYLGGFRNSMSFVLTGLDVE
                     AKAAMAEAALFERLGGRERFQEAESRLVGSPADDPDSAEASVAELRITVKDPDPAKVG
                     RAFSNAAIELALASYPGCYTTTPPGDATPYGIYWPALVRADLVEQVVVLPDGRRLPVK
                     PPPGRAPGPPGSPGALQPRPPTPGPPMGERSPTPVSGDP"
BASE COUNT          296 a          981 c         1051 g          317 t
ORIGIN      
        1 ccgggggacc cggcgcggga ccgggtctgt tcggcgcggg cgaccccacg ggtggccgcg
       61 caggcgtccc cacgggccgc ccggcggacg tccccacggg cggccccgcg gatggcgtcg
      121 cgagcggccc tgccggtgat cccgtgggcg gcctcggagg cggcgtgggc gaacgcgggg
      181 cgaccggcct gccgcacggg gccctggtgt cgccgctgcc aggggttgtg cggcgggtgg
      241 aggtgcgggc cggggaccgg gtcgaggccg gggcggtgct ggtggtggtc gaggcgatga
      301 agaccgagca ccggatcgcg gccccgcggg ccgggagggt ggggcgggtg ctggtctccg
      361 agggtcagga ggtggccgcc ggggccgcgg tggccgagct ggaggagccg ggcgatggct
      421 gaggcggtct cctacgagct ccaggacggc gtggtctggc tggtgatcga gcggccggag
      481 gcgcgcaacg ccatcaaccg ggccgtcgcc gacggcctct gggaggggtt ccggcggttc
      541 gaggccgacc cggacgcggc cgtgctggtg ctcaccggcg ccggggacgc gttctgtgcc
      601 ggcgcggacc tcaaggagat ggccgccctc ggcctgaccg tgccgccgcg cgacatgacc
      661 ccgaacctcg gccggaacat cgaggtgacc aagccggtgg tggccgccgt caacgggccg
      721 gcattcggcg gcgggttcct gctggcgcag atgtgcgacc tctgtgtggc cggcacctcg
      781 gcccggttcg cgatcaccga ggcacgctgg ggccgggggg cgccgtgggc ggcgccgctg
      841 ccgtggctgg tcccgccgcg ggtggcgctg gagctgctgc tgaccggcga gccgatcgac
      901 gcccgccggg cctacgaggt cgggctggtc aaccgggtcg tgcctgaccc ggagctccgc
      961 tccgaggccg gccgcctggc ccggcggatc gccgacaatg cgccgctgtc ggtgcgggcg
     1021 gccaaggcca tggtccacgc caccgccggg cgcgcgctcg acgaggcggt ggaggagggc
     1081 tggcggctgt tcgagccggt gtacctgagc gaggacgccc aggagggacc gcgggcgttc
     1141 gtggagaagc gcccgccggt ctggaagggc cgctgaggca tggagccgct gcgcatcgcc
     1201 aactgctccg gcttctacgg cgaccggcgc agcgccccgc gggagctgct cgagggcggc
     1261 ccggtcgacg tcctcaccgg tgactacctg gccgagctga ccatgctgat cctgtggcgc
     1321 tcgcgcagcc gcgacccggc caaggggtac gcgaccagct tcctcgccca gatggaggag
     1381 tgcctcggcc tggccgccga caagggggtc aaggtggtgg ccaacgccgg cggcctcaac
     1441 ccggccggcc tggccgccga gctgcgggcg ctcgccggtc ggctgggcct ggacgtcgtc
     1501 gtcgcccacg tcgagggcga cgacctgctc ggccgcctcg acgacctccg ggcggcgggg
     1561 gagccgctgg cccacctcga cagcggcgtg ccgctggccg cggccggggt ggtgccggta
     1621 accgccaacg cctacctggg cgcctggggg atcgtggccg cgctggaggc cggcgccgac
     1681 gtggtcgtgt ccggccgggt cgccgacgcc tccctggtcg ccgggccggc cgcctggcac
     1741 ttcggctggg ccgccgacga ctgggaccgg ctcgccgggg cggtggttgc cggccacgtg
     1801 ctggagtgcg ggacccaggc caccggcggc aactacgcct tcctggacga gctgcccggc
     1861 acccgcccgc ccggcttccc gatcgccgag gtggccgccg acggcagcgc cgtcgtgacc
     1921 aagcacccgg ggacgggcgg gctggtctcg gccggcacgg tgaccgccca gctgctgtac
     1981 gagatcgccg ggccggccta cgccagcccc gacgtggtcg cccgcttcga cacggtgagg
     2041 atcgaggagg tcgggccgga ccgggtgcgc ctgtccggca cccgcggcga gcccgccccc
     2101 gaccggctga aggtggccgt caactacctc ggcgggttcc gcaactcgat gagcttcgtc
     2161 ctcaccggcc tcgacgtgga ggccaaggcg gccatggcgg aggcggccct gttcgagcgc
     2221 ctcggcggcc gcgagcggtt ccaggaggcc gagagccgcc tggtcggcag ccccgccgac
     2281 gacccggaca gcgccgaggc gtccgtcgcc gagctccgga tcacggtcaa ggaccccgac
     2341 cccgccaagg tcggccgcgc cttctccaac gccgccatcg agctcgccct ggccagctac
     2401 cccggctgct acaccaccac cccgcccggc gacgccaccc cctacggcat ctactggccg
     2461 gccctggtcc gcgccgacct ggtcgagcag gtggtcgtcc tccccgacgg ccgccgcctc
     2521 ccggtcaagc cgccgcccgg tcgcgcgcca ggtccacccg ggtccccggg cgcgctccag
     2581 cctcgcccgc cgacaccagg gcccccgatg ggggagcgta gcccaacgcc ggtctcgggg
     2641 gatcc
//
LOCUS       DASSWR010000064         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_567594,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000064 DASSWR010000000
VERSION     DASSWR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567594"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1092)
                     /locus_tag="VFO47_01080"
     CDS             complement(4..1092)
                     /locus_tag="VFO47_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET8787323.1"
                     /translation="MTADPRRYRLGVLEGDGIGPEIVPAAVRVVDAATAAVAGRPTVD
                     WVPLPLGAAAIDEYGTAVPDKTLRALAELDGWLLGPHDSASYPEPHRSALNPSGTIRK
                     HFDLFANIRPARGFEGARAISPDTDLIIVRENTQGFYADRSTYAGTGEFMPSPDVAIA
                     LGIVTRPAVERIAEVACDLAARRRKHLTIVHKANVLKLTTGLFRDVCREVAAGYPDVT
                     VDDFHIDAMTVHLVRRSPDFDVLVTENMMGDILSDLAGEIAGSLGIAPSINASADLAM
                     AQAAHGSAPDIAGRDLANPIAMISSSAMLLEWLGARHDDEAATRAAALVEQAVTETVE
                     GGVATRDLGGTASTSGFTTAVVEAIGRR"
     gene            1254..1949
                     /locus_tag="VFO47_01085"
     CDS             1254..1949
                     /locus_tag="VFO47_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET8787324.1"
                     /translation="MEIARRLLDYFLSGEVEPGERIPSERRLAESLGVGRSLVREALK
                     SLHLLGLLEVRQGDGTYLKRTDSELLPQVIEWGLLLGERPALDLVEARAHLEVVVAGL
                     AAERRDEQALDDLRGLLETMRKSSDDPVRFVEADVGFHLRVAEAAGNVVLNDMLNGVR
                     ALLRVWIRRVIEAAGKTGPSYREHLPIFEAIERGDREAAVAAMAAHMEGASARLKASI
                     SQSAWSNEAAPDG"
BASE COUNT          268 a          687 c          773 g          284 t
ORIGIN      
        1 ccgtcaacgg cgcccgatgg cctcgaccac ggccgtggtg aagccgctgg tggaggccgt
       61 gccgccgagg tcgcgggtgg ccacgccgcc ctcgacggtc tcggtcacgg cctgctcgac
      121 cagggccgcg gcccgggtgg ccgcctcgtc gtcgtggcgg gcacccagcc actccagcag
      181 catcgcgctg gagctgatca tcgcgatcgg gttggccagg tcgcgcccgg cgatgtcggg
      241 ggccgagccg tgggcggcct gggccatggc caggtcggcc gaggcgttga tcgagggggc
      301 gatgccgagc gatccggcga tctcaccggc caggtcggag aggatgtcgc ccatcatgtt
      361 ctcggtgacc agcacgtcga agtcgggcga gcgccggacc aggtgcacgg tcatggcgtc
      421 gatgtggaag tcgtcaacgg tcacgtccgg gtagccggcc gcgacctcgc ggcacacgtc
      481 gcggaacagc ccggtggtca gcttgagcac attggccttg tggacgatgg tcaggtgctt
      541 gcggcggcgg gcggccaggt cgcaggcgac ctcggcgatc cgctcgacgg ccggccgggt
      601 gacgatgccg agggcgatgg ccacgtccgg ggacggcatg aactcgccgg tgccggcgta
      661 ggtgctgcgg tcggcgtaga agccctgggt gttctcgcgc acgatgatga gatccgtgtc
      721 gggagagatg gcccgggcgc cctcgaagcc cctggccggg cggatgttgg cgaacaggtc
      781 gaagtgcttg cggatggtgc cgctgggatt gagggccgag cggtggggct cggggtagct
      841 ggcgctgtca tggggcccga gcagccagcc gtccagctcg gccagggccc ggagggtctt
      901 gtcggggacg gcggtgccgt actcgtcgat ggcggcggcg cccagcggca gcggcaccca
      961 gtcgaccgtc ggccggccgg cgacggccgc cgtggccgcg tccaccaccc gcacggccgc
     1021 gggcacgatc tccgggccga tgccgtcgcc ttcgaggacg ccgagccggt agcgtcgcgg
     1081 gtctgcggtc atgtcgcacc tccgttcggc cggtccgggt ggcgggcggc catggtgccg
     1141 gtgaattggt cgacctgtca accactcgac cggcgggcca aaccgcgtac gatacgaaca
     1201 ccatgccgac cggtctaccc agaatcgacc tcagctcgcg cgagacgctc acgatggaga
     1261 tcgcgcgccg gctgctcgac tacttcctgt ccggagaggt cgagcccggg gagcggatcc
     1321 cctccgagcg ccgcctggcc gagtccctcg gcgtcggccg ctcgctggtc cgggaggcgc
     1381 tcaagtcgct gcacctgctc ggcctgctcg aggtgcgcca gggcgacggc acctacctga
     1441 agcggaccga ctccgagctg ctgccgcagg tgatcgagtg gggcctgctg ctgggcgagc
     1501 ggccggccct ggacctggtc gaggcccgcg cccatctcga ggtggtggtg gccggcctgg
     1561 ccgccgagcg ccgcgacgag caggccctcg acgacctgcg agggctgctg gagacgatgc
     1621 ggaagtcgag cgacgacccg gtccgcttcg tcgaggccga cgtcggcttc cacctgcggg
     1681 tggccgaggc ggccggcaac gtggtcctga acgacatgct caacggcgtg cgcgccctgc
     1741 tgcgggtctg gatccgccgg gtgatcgagg ccgccggcaa gaccggcccc tcctaccggg
     1801 agcacctccc gatcttcgag gcgatcgagc ggggcgaccg cgaggcggcc gtcgcggcca
     1861 tggccgccca catggagggg gccagcgccc gcctcaaggc ctcgatctcc cagagcgcct
     1921 ggtccaacga ggccgcgccc gacggttgag cctcatcctg gccgacgctt cgaccacggc
     1981 gcgagggtga tccagagggg cctcgacttc gg
//
LOCUS       DASSWR010000065         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_256856,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000065 DASSWR010000000
VERSION     DASSWR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256856"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1373)
                     /locus_tag="VFO47_01090"
     CDS             complement(159..1373)
                     /locus_tag="VFO47_01090"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET8787325.1"
                     /translation="MTRPTADLVVLAYSGGLDTTVTVHRLVHEQGRAVVALLADVGQG
                     EDLPALAERALAAGAADAVVVDARDRFAAEFLVPAVRANALYEGRYPLVSALSRPLIA
                     QLLVEEARRRGAGAVAHGCTGKGNDQVRFEVSCGALAPELEILAPVRDSAMSRPEAIS
                     YADKHGIDLGWITEAKPWSVDQNLWGRTVEAGVLEDPWLAPPEEAFEWTASPATASGA
                     REVVVGFEGGVPATVDGAEVGLAGVVAALNQAGGQVGFGRVDMMENRRVGIKSREVYE
                     VPGALAVIAAHRDLEDLTLDRDVLHEKARLDIRYAELVYDGLWFSPLKRALDAFIEDA
                     NSHVTGDIRMTLHGGRAVVTGRRSEESLYDFALATYDAEDTFDQTLAKGFVELWGLPS
                     KIAAKRDQASGS"
     gene            complement(1370..1882)
                     /gene="argR"
                     /locus_tag="VFO47_01095"
     CDS             complement(1370..1882)
                     /gene="argR"
                     /locus_tag="VFO47_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET8787326.1"
                     /translation="MSLKAARQQALAALLRTRQVSSQALVLEHLRAQGFDATQATISR
                     DLDDLGAVKVRGPDGRLVYALPEPGNGQGADHDEVRRMLGASLLAIVPSGNLVVLRTP
                     PGHAAALASTLDRAGIAGVAGTVAGDDTILVVCTERTPGRVMARQLAALAVTPPELPA
                     ISEPYRGATA"
     gene            complement(1873..3132)
                     /locus_tag="VFO47_01100"
     CDS             complement(1873..3132)
                     /locus_tag="VFO47_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HET8787327.1"
                     /translation="MSSLPEREAAVLWPTYARPQVEFVRGRGVHLWDARGREYLDFLG
                     GIAVVAAGHAHPKVVRAVTAQLGTLGHTSNLYFTGPQVELAERLVGHFGGDAKVFLSN
                     SGAEANEAAIKVARRWGRANRGEQATGIVATLGGFHGRTLATLAATGKPAMHTPFQPL
                     PPGFSHVPFGDVDALAAAIGPDTAAVLIEPIQGEAGIRVPPPGYLRAVRELTSRAGVL
                     LIVDEIQSGMGRTGRFFAHQHDEIRPDVVTMAKALGSGYPIGATIARTEVADAMQRGD
                     HGTTFGGNPVACTAALATLDVVEPLLAGHVDKVSTRLGDGLQELRRRLTGRSTWAPPS
                     ADDADDGLPGAEVRGAGLWFALDLGPEAPADAKAAALAALGRGLVVNPVTDTALRLAP
                     PLVVTEDEIDEGLARLGAALEEAGLCR"
     gene            complement(3129..4082)
                     /gene="argB"
                     /locus_tag="VFO47_01105"
     CDS             complement(3129..4082)
                     /gene="argB"
                     /locus_tag="VFO47_01105"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET8787328.1"
                     /translation="MTTAQPATGPVQRRPVGSGHAVPTLDDLQAKAAAVHEALPWIKR
                     LSGRTVVVKYGGAAMGTAGFDAFVDDVVLLRYVGVDVIVVHGGGPEVSQLMARFGKEP
                     VFVSGHRVTDAETMDLARMVLVGRVNKTLVGRINTHGRLAVGLSGEDGLLLRARPRVD
                     PGGADLGYVGDVEAVDPTALRALTDKGLVPVVATVAAGPGGQPYNVNADAAAGSLAAA
                     LGAEKLVYLTDVEGLYADLADPGSLLQQLTVAEVEAILAGGEVAAGMVPKLTGIVTAL
                     RGGVRRAHILDGRREHALLLELFTDSGVGTMVAAEDEEERS"
     gene            complement(4079..5329)
                     /gene="argJ"
                     /locus_tag="VFO47_01110"
     CDS             complement(4079..5329)
                     /gene="argJ"
                     /locus_tag="VFO47_01110"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET8787329.1"
                     /translation="MSTSVQVDRRLGVTAAAGFGAAGVTCGLKASGAPDLALVVGDPG
                     TVAAGVFTTNQVVAAPVTWSRDRLAASGDARVVVLNSGNANACTGPAGDAAVLATVER
                     AAAELGCRAEQVLVCSTGVIGVPLQVARVADGVAKAAAALDRSGGLAAAEAILTTDTV
                     TKQAGGRVAGGVTVGGMAKGAAMLAPELGPALAHATMLAVLTTDAVAAVEVLREALEA
                     GVATTFNRITVDGAQSTNDTVLLLASGASGVRADPGELAEAVRAVCRDLAGQLLADAE
                     GGTKVVSVQVGGAGGEEQALAVARRVADSPLVKTAVYGGDPNWGRVLQAAGTAGVGFD
                     PGLVRLEVAGGPGAAGIEPAAGPRERVLLVSQGRPTGADAGAAMAATEIELRLHLGGS
                     GPWAEVRTSDLTPEYVRLNSEYTT"
     gene            complement(5326..>5509)
                     /locus_tag="VFO47_01115"
     CDS             complement(5326..>5509)
                     /locus_tag="VFO47_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET8787330.1"
                     /translation="TLGSNSCHLQVSTDLDSGRVIAVSALDNLGKGAAGQAVQCANLA
                     LGLPETAGLDLVPPGP"
BASE COUNT          658 a         2126 c         2055 g          670 t
ORIGIN      
        1 aatcgcagcg cgagcctgcg agcgcgagaa tctgcccagc tcccgccggg tgtcggcgca
       61 gccgccgccc ccggccaccc cgggcaccgg cgccgccgcc gcccaagccc accccgggtg
      121 ccggcgcagt cagagcgccg gcccccgggg tgcacaggtc atgagcccga ggcctggtcc
      181 cgcttggccg cgatcttcga cggcaggccc cacagctcga cgaagccctt ggccagcgtc
      241 tggtcgaacg tgtcctccgc gtcgtacgtc gcgagcgcga agtcgtacag cgactcctcg
      301 ctgcgccggc cggtcaccac cgcgcgtccc ccgtgcagcg tcatgcggat gtcgccggtg
      361 acgtgggagt tggcgtcctc gatgaaggcg tcgagcgccc gcttgagcgg cgagaaccac
      421 agcccgtcgt agaccagctc ggcgtagcgg atgtcgaggc gggccttctc gtggaggacg
      481 tcgcggtcca gggtcaggtc ctccaggtcg cggtgggcgg cgatcaccgc cagggcgccc
      541 gggacctcgt agacctcgcg gctcttgatc cccacccggc ggttctccat catgtcgacc
      601 cgcccgaacc ccacctggcc tccggcctgg ttgagggcgg cgacgactcc ggccagcccg
      661 acctcggccc cgtcgaccgt cgccggcacc ccgccctcga agccgaccac cacctcgcgg
      721 gcgccgctgg ccgtggccgg cgaggccgtc cactcgaagg cctcctccgg cggggccagc
      781 cacgggtcct caaggacccc ggcctcgacg gtccggcccc acaggttctg gtcgaccgac
      841 cacggcttgg cctcggtgat ccagcccagg tcgatgccgt gcttgtcggc gtaggagatc
      901 gcctccggcc ggctcatggc cgagtccctg accggggcca ggatctccag ctcgggggcg
      961 agggcgccgc agctgacctc gaaccggacc tggtcgttgc ccttgccggt gcagccgtgg
     1021 gcgaccgcgc ccgccccccg gcgccgggcc tcctcgacca gcagctgggc gatcagcggc
     1081 cgcgacaggg ccgacaccag cgggtagcgg ccctcgtaga gggcgttggc ccgcaccgcc
     1141 gggaccagga actcggccgc gaagcggtcc cgggcgtcca ccaccacggc gtcggccgcc
     1201 ccggcggcca gggcccgctc ggccagcgcc ggcaggtcct cgccctggcc cacgtcggcc
     1261 agcagggcga ccacggcgcg accctgctcg tggaccagcc ggtgcacggt cacggtcgtg
     1321 tccagcccgc ccgagtaggc gagcaccacg aggtcggcgg tgggtcgggt catgcggtgg
     1381 ctcctcggta gggttcggag atggcgggca gttcgggcgg ggtcaccgcc agggcggcga
     1441 gctggcgggc catgacccgg ccgggggtcc gttcggtgca gacgaccagg atggtgtcgt
     1501 ccccggcgac ggtgccggcg accccggcga tgccggcccg gtcgagagtg gaggcgagcg
     1561 cggccgcgtg cccgggcggg gtgcggagca cgaccaggtt gccgctgggc acgatggcca
     1621 gcagcgacgc cccgagcatg cggcggacct cgtcgtggtc ggcgccctgg ccgttgcccg
     1681 gctcgggcag ggcgtagacg agccggccgt ccggcccccg gaccttgacc gcgcccaagt
     1741 cgtcgaggtc gcgcgagatg gtcgcctggg tggcgtcgaa gccctgggcg cgcaggtgct
     1801 ccaggaccag ggcctggctg gacacctgcc gggtccggag cagcgccgcc agggcctgct
     1861 ggcgggccgc cttcagcgac acaggccggc ctcctccagg gccgcgccca gccgggccag
     1921 cccctcgtcg atctcgtcct cggtgaccac caggggcggg gccaggcgca gggcggtgtc
     1981 ggtgaccggg ttgacgacca gcccgcggcc gagcgcggcc agggcggccg ccttggcgtc
     2041 ggccggcgcc tcgggaccga ggtcgagggc gaaccacagg cccgctcccc ggacctcggc
     2101 tccggggagc ccgtcgtcgg cgtcgtcggc gctcggcggg gcccaggtgg agcggccggt
     2161 gaggcgtctc cgcagctcct ggagcccgtc gcccagccgg gtcgacacct tgtcgacgtg
     2221 gccggcgagc agcggctcga ccacgtccag ggtggccagg gcggcggtgc aggcgaccgg
     2281 gttgccgccg aaggtggtgc cgtggtcgcc gcgctgcatg gcgtcggcga cctcggtcct
     2341 ggcgatggtg gcgccgatcg ggtagccgga gccgagcgcc ttggccatgg tgaccacgtc
     2401 gggccggatc tcgtcgtgct ggtgggcgaa gaagcggccg gtgcggccca tcccggactg
     2461 gatctcgtcc acgatcagca gcaccccggc ccggctggtg agctcccgga cggcgcggag
     2521 gtagccgggg ggcgggaccc ggatgccggc ctcgccctgg atcggctcga tcagcacggc
     2581 cgccgtgtcc ggcccgatgg ccgcggccag ggcgtccacg tccccgaagg gcacgtggga
     2641 gaacccgggc gggagcggct ggaacggcgt gtgcatcgcc ggcttgccgg tggcggccag
     2701 agtggccagg gtgcggccgt ggaagccgcc cagggtggcc acgatgccgg tggcctgctc
     2761 gccccggttg gcccggcccc agcgccgggc caccttgatg gcggcctcgt tggcctcggc
     2821 gcccgagttc gacaggaaca ccttggcgtc gccgccgaag tggccgacca gccgctcggc
     2881 cagctccacc tgggggccgg tgaagtagag gttggaggtg tggccgaggg tgccgagctg
     2941 ggccgtcacc gcccgcacga ccttcgggtg ggcgtggccg gcggccacca ccgcgatccc
     3001 gcccaggaag tcgaggtact cgcggccgcg cgcgtcccac aggtgcaccc cccggccgcg
     3061 gacgaactcg acctggggcc gcgcgtaggt cggccacagc acggccgcct cgcgctcggg
     3121 caggctgctc acgaccgctc ctcctcatct tccgcggcga ccatggtccc gaccccggag
     3181 tcggtgaaca gctccagcag cagggcgtgc tcgcgccggc cgtcgaggat gtgggcccgg
     3241 cggaccccgc cgcgcagggc ggtcacgatc ccggtgagct tgggcaccat cccggccgcg
     3301 acctcgccgc cggccaggat ggcctcgacc tcggccacgg tgagctgctg gagcagcgag
     3361 cccgggtcgg ccaggtcggc gtagagcccc tccacgtcgg tcaggtagac cagcttctcg
     3421 gcgccgaggg cggccgccag cgacccggcg gcggcgtcgg cgttgacgtt gtagggctgg
     3481 cccccggggc cggcggccac cgtggccacc accgggacca gacccttgtc ggtgagggcg
     3541 cgcagcgcgg tcgggtcgac cgcctcgacg tcgccgacgt agccgaggtc ggcgccgccc
     3601 gggtcgaccc ggggcctggc ccggagcagc aggccgtcct cgccggacag gccgacggcc
     3661 aggcggccgt gggtgttgat gcggcccacc agggtcttgt tgacccggcc gaccagcacc
     3721 atgcgggcca ggtccatggt ctcggcgtcg gtgacccggt ggccgctgac gaacaccggc
     3781 tccttgccga agcgggccat gagctggctg acttcggggc cgccgccgtg gacgacgatc
     3841 acgtcgacgc ccacgtagcg gagcaggacg acgtcgtcga cgaaggcgtc gaagccggcc
     3901 gtgcccatgg ccgccccgcc gtacttgacg accaccgtgc ggcccgacag ccgcttgatc
     3961 cagggcaggg cctcgtggac ggcggccgcc ttggcctgca ggtcgtccag ggtcggaacg
     4021 gcgtgcccgg accccaccgg acgccgctgg accggacccg tcgccggctg ggccgttgtc
     4081 atgtcgtgta ctccgagttg aggcggacgt actcgggggt caggtcgctg gtccggacct
     4141 cggcccatgg cccgctcccg cccaggtgca gccgcagctc gatctccgtg gccgccatgg
     4201 ccgcgcccgc gtccgccccg gtgggccgcc cctggctgac gagcaggacc cgctcccgcg
     4261 gcccggccgc cggctcgatc ccggccgccc ccggcccgcc ggccacctcc agccgcacca
     4321 gccccgggtc gaacccaacc ccggccgtgc ccgcggcctg cagcacccgc ccccagttgg
     4381 ggtcgccccc gtacacggcc gtcttgacca gcgggctgtc ggcgacccgc cgggccacgg
     4441 ccagcgcctg ctcctctcca ccggcccctc cgacctgcac gctgaccacc ttggtcccgc
     4501 cctcggcgtc ggccagcagc tgcccggcca ggtcgcggca gaccgcccgc acggcctcgg
     4561 ccagctcccc cgggtcggcc cggacccccg acgcccccga ggccagcagc agcaccgtgt
     4621 cgttggtcga ctgggccccg tccaccgtga tccggttgaa ggtggtcgcg acgcccgcct
     4681 ccagcgcctc ccgcagcacc tccacggcgg cgaccgcgtc ggtggtcagg acggccagca
     4741 tggtcgcgtg ggccagcgcg ggcccgagct ctggggccag catggccgcc cccttggcca
     4801 tgccgcccac cgtgacccct ccggcgaccc gcccgccggc ctgcttggtc acggtgtcgg
     4861 tggtgaggat ggcctcggcc gcggccagcc cgcccgaacg gtcgagggcg gccgcggcct
     4921 tggcgacccc gtcggcgacc cgggccacct gcagcggcac cccgatgacc ccggtggagc
     4981 agaccaggac ctgctcggcc cggcagccca actcggccgc ggcccgctcc acggtggcca
     5041 ggacggccgc gtccccggcc ggtccggtgc aggcgttggc gttgcccgag ttgagcacga
     5101 ccacccgggc gtcgcccgag gccgccagcc ggtcgcgcga ccaggtgacc ggcgccgcca
     5161 ccacctggtt ggtggtgaac accccggcgg ccaccgtccc cgggtcgccc acgaccaggg
     5221 ccaggtcggg cgcccccgac gccttgagcc cgcaggtcac cccggccgcc ccgaacccgg
     5281 cggccgcggt caccccgagc cgccggtcga cctgcacgga agtgctcacg gtcccggcgg
     5341 gacgaggtcg aggccggccg tctcaggcag gccgagggcg aggttggcgc actggacggc
     5401 ctggccggcg gcgcccttgc cgaggttgtc gagggcgctg accgcgatca cccgcccgga
     5461 gtcgaggtcg gtgctcacct gtaggtgaca ggagttggag ccgagggtc
//
LOCUS       DASSWR010000066         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_341465,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000066 DASSWR010000000
VERSION     DASSWR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341465"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFO47_01120"
     CDS             <1..222
                     /locus_tag="VFO47_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787331.1"
                     /translation="ADLRRTLEADGISGRPVFVVSADPPDGHDHGELEALRAWLSERL
                     QVKAVVTERLAAEARQRGVELARQAGLEG"
     gene            280..1047
                     /locus_tag="VFO47_01125"
     CDS             280..1047
                     /locus_tag="VFO47_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787332.1"
                     /translation="MAAARSAVDVEGVRRACRRRTLAEARAAGAGPLGRLLAAVARAR
                     GAGDRGPASARSIDPVAYARAWRSRSTLSRTVNPVHDLLRRAATAAPPALRAEVMGLA
                     RPERLEERVTDAIDGAVARGTVDHARPPRSFLWPLIGVLQTLVTVAFAAGLLWYLTLY
                     LAGRAQADLPDLPTVRDVPVPLILVVGSLLAGWLLSRLLSASARRAGRRWADRLTGDL
                     DATVAAEVEAAMSEPLAELEAARSELLVALSDLRDAC"
     gene            complement(1044..2252)
                     /locus_tag="VFO47_01130"
     CDS             complement(1044..2252)
                     /locus_tag="VFO47_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787333.1"
                     /translation="MRNFRLFLFGHLVSSTGTWMQQVGQDWLVLRLTDAALPLGITLA
                     LQFAPMLAFGAWAGLVADRADKRRLLLVTQTAMAVLALALGALTATGAVRLWMVYALA
                     LLLGVATAFDMPARQAFVSEMVGPDRVANAVGLNSASFNTARVVGPAVAGALIAVVGI
                     APAFFINAVSYLAMIGGLLAMDPDRLYRRPAEERGRGQIRAGVRYVWATPVLRSTVLL
                     VAVVGMFGLNYRVALPLLARFAFDGGPGAYGALAAIMAAGSVVGALVVARRGRPSRLL
                     LVGSVAAFGVLSFAAAAAPNILVEAAVLFPIGAASLAFVATANSTVQLASDPEKRGRV
                     MSLFGLVLLGSSLPSGLLSGWLAGQFGPRSILLLSGVSCVLAAAVAAFTARSRVERSP
                     ALPGDAERAA"
     gene            complement(2304..>2669)
                     /locus_tag="VFO47_01135"
     CDS             complement(2304..>2669)
                     /locus_tag="VFO47_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787334.1"
                     /translation="VALDELPERRRRLLEAWREAGRDPETARLSLMTTCVAGRDRAEV
                     EERVGRVLAVTGSHDSVAEVIDARPNWLLGTVDELAERFAALEAAGVGRVMLQHLDHA
                     DTDMVAVLGEVAATLGSTP"
BASE COUNT          344 a         1023 c         1031 g          271 t
ORIGIN      
        1 gccgacctgc gccgcaccct ggaggccgac ggcatctcgg ggcggccggt gttcgtggtc
       61 tcggccgacc cgcccgacgg ccacgaccac ggcgagctgg aggcgctgcg ggcctggctg
      121 tcggagcggc tccaggtcaa ggcggtggtg accgagcggc tggccgccga ggcccgccag
      181 cgcggggtcg agctggcccg ccaggccggc ctggagggct aggcggcgag gcggccgctg
      241 gtcggcgacg ccgcccgccg ggacgcgcgg cgggcggcgg tggccgcggc ccgctcggcc
      301 gtcgacgtcg agggggtgcg ccgggcctgc cggcgccgca ccctggccga ggcccgggcc
      361 gccggtgccg gccccctcgg ccggctgctg gccgccgtgg cccgggcccg cggcgccggc
      421 gatcgtggcc ccgcctcggc ccgctcgatc gacccggtcg cctacgcccg cgcctggcgg
      481 agccgctcga ccctcagccg caccgtcaac ccggtccacg acctgctccg ccgggcggcc
      541 acggccgccc ctccggccct gcgggccgag gtcatgggcc tggccaggcc cgagcggctg
      601 gaggagcgcg tcaccgacgc catcgacggg gcggtggcca ggggcacggt cgaccacgcc
      661 cgcccgcccc ggtcgttcct ctggcccctc atcggcgtgc tccagaccct ggtcacggtc
      721 gccttcgccg ccggcctgct ctggtacctc accctctacc tggccggccg ggcccaggcc
      781 gacctccccg acctccccac cgtccgcgac gtccccgtcc ccctcatcct ggtcgtcggc
      841 agcctgctgg ccggctggct gctgtcccgc ctgctgtcgg ccagcgcccg ccgcgccggc
      901 cgccgctggg ccgaccgcct caccggcgac ctcgacgcca ccgtcgccgc cgaggtcgag
      961 gcggccatgt ccgaacccct ggccgagctg gaagcggccc gttcagagct cctggtcgcc
     1021 ctctccgacc tgcgggacgc ctgctaggcg gcccgctcgg cgtcgccggg gagggccggg
     1081 gagcgctcga cccggctccg ggcggtgaac gcggccacgg cggcggccag gacgcaggag
     1141 accccggaga ggagcaggat cgagcgggga ccgaactggc cggccagcca gccgctgagg
     1201 aggccgctgg ggaggctgga gccgagcagg acgaggccga acaggctcat cacgcggccg
     1261 cgcttctcgg ggtcggaggc gagctggacg gtcgagttgg ccgtggccac gaaggccagg
     1321 ctggccgcgc cgatggggaa caggaccgcc gcctcgacca ggatgttggg ggcggcggcg
     1381 gccgcgaacg acagcacgcc gaaggcggcc accgacccga ccagcagcag ccgcgagggc
     1441 cggccccggc gggcgacgac cagggcgccg acgaccgagc cggcggccat gatggcggcc
     1501 agggccccgt aggcgccggg gccgccgtcg aaggcgaacc gggccagcag cggcagggcc
     1561 acccggtagt tgaggccgaa catgccgacg acggccacca gcaggacggt cgagcgcagc
     1621 accggggtcg cccagacgta gcgcacgccg gcccggatct ggccccggcc ccgctcctcg
     1681 gcgggccggc ggtacaggcg gtcggggtcc atggccagca ggccgccgat catggccagg
     1741 taggagacgg cgttgatgaa gaaggcgggg gcgatcccga ccaccgcgat cagcgccccg
     1801 gcgacggccg ggccgacgac ccgggcggtg ttgaagctgg cgctgttcag cccgacggcg
     1861 ttggcgaccc ggtcgggccc gaccatctcg ctgacgaagg cctgcctggc cggcatgtcg
     1921 aaggcggtgg ccaccccgag cagcagggcc agggcgtaga ccatccacag ccggaccgcc
     1981 ccggtcgcgg tcagggctcc gagggcgagg gcgagcacgg ccatggcggt ctgggtgacc
     2041 agcagcagcc ggcgcttgtc ggcccggtcg gcgaccagcc ccgcccaggc cccgaaggcg
     2101 agcatgggcg cgaactggag ggccagggtg atgccgagcg gcagggccgc gtcggtcagg
     2161 cgcaggacca gccagtcctg gccgacctgc tgcatccagg tgccggtgga ggagacgagg
     2221 tgaccgaaca ggaacaaccg gaagttgcgc acgccgaggg agtcgaaggt gcgcagccgg
     2281 gtcgacctcc aggccaggac gggttacggg gtggagccca gagtagcagc gacctccccg
     2341 agcaccgcga ccatgtcggt gtcggcgtgg tcaaggtgct ggagcatgac ccggccgacc
     2401 ccggccgcct ccagggccgc gaaccgctcg gcgagctcgt cgaccgtgcc gagcagccag
     2461 ttgggccggg cgtcgatcac ctcggccaca ctgtcgtggc tgccggtgac ggccaggacc
     2521 cggccgaccc gctcctccac ctcggcccgg tcgcggccgg ccacacaggt ggtcatcagc
     2581 gacagccggg ccgtctcggg gtcccggccg gcctcgcgcc aggcctccag cagccgccgg
     2641 cgccgctccg gcagctcgtc cagggccac
//
LOCUS       DASSWR010000067         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1245794, whole genome shotgun sequence.
ACCESSION   DASSWR010000067 DASSWR010000000
VERSION     DASSWR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245794"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFO47_01140"
     CDS             <1..487
                     /locus_tag="VFO47_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8787335.1"
                     /translation="DALALADELGLERLVSNQPEYSIVRRRIEAEVLPLSAREGIGQV
                     VWSPLAQGVLTGKYRKGQAPPSDSRAASRKMGSFVGRFLTDDVLDAVERLRVVADEAG
                     LTMAQLALAWVLRTPTVASAIVGASRPEQLEDNCAAAGRRLDDEVLAAVDKAVAGVLE
                     P"
     gene            493..1329
                     /gene="lgt"
                     /locus_tag="VFO47_01145"
     CDS             493..1329
                     /gene="lgt"
                     /locus_tag="VFO47_01145"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET8787336.1"
                     /translation="MPVLAFIPAPPTNGFQLGPFFFHLYGLCIAVGVLAAFWLANRRW
                     VQAGGQPGELERPAIWAVIAGFLGGRLGYVITHTGDFRDNPLEVFAIWQGGLALYGGL
                     TVGILTGLWVARRRGLPVLKALDAAIPAIPLAQAIGRWGNYFNQELFGTPTTVPWALE
                     VDPEHRPDRYASFATFHPTFLYESIYNLGVVVVLLRVSATRRLRPGSLSLLYLVLYGA
                     GRFGLELLRTDTTYRLGGLSRNAWISLALVVGGSVWLWLRERRRPAAEADDDAEERQW
                     SS"
     gene            1317..2081
                     /gene="map"
                     /locus_tag="VFO47_01150"
     CDS             1317..2081
                     /gene="map"
                     /locus_tag="VFO47_01150"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET8787337.1"
                     /translation="MVILKSRDEIATMRRAGRVVARTLERVVAAVRPGVSLRELDDLG
                     ARTIAAEGATASFLGYHPRFAPTPFPASLCLSVNEVIVHGIPDRRRLRAGDVLSIDCG
                     AVVDGYHADAAVTVPVGEIDPAAARLLEATERALWAGIEQARVGNRLSDISHAVGQVA
                     RAAGYGILADHGGHGVGQLLHEDPSVDNDGRPGRGPRLRDGLVLAIEPMFVDGDPAYV
                     HRPDGWAVATRDGSRSAHFEHTVAVLDEGPEPLTVP"
     gene            2119..>2270
                     /locus_tag="VFO47_01155"
     CDS             2119..>2270
                     /locus_tag="VFO47_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787338.1"
                     /translation="MRLLEREPALEALGGWLAEAGAGRGRLVLVAGEAGVGKTALVEE
                     FTVRQRP"
BASE COUNT          250 a          870 c          840 g          310 t
ORIGIN      
        1 cgacgccctc gccctggccg acgagctggg ccttgaacgg ctggtgtcca accagcccga
       61 gtactcgatc gtgcgccggc gcatcgaggc cgaggtgctg cccctgtcgg cccgggaggg
      121 catcggccag gtcgtctggt cgcccctggc ccagggggtg ctgaccggca agtaccgcaa
      181 gggccaggcg ccgccgtccg acagccgggc cgcgtcgcgc aagatgggct cgttcgtggg
      241 ccggttcctc accgacgacg tcctggacgc ggtcgagcgg ctgcgggtgg tcgccgacga
      301 ggccggcctg accatggccc agctcgccct ggcctgggtg ctgcgcaccc cgacggtggc
      361 gtcggcgatc gttggcgcgt cccggcccga gcagctggag gacaactgcg ccgccgccgg
      421 ccgccgcctg gacgacgagg tgctggccgc cgtggacaag gccgtggccg gcgtgctgga
      481 gccgtgagcc ggatgccggt cctggccttc atcccggcgc cgccgaccaa cgggttccag
      541 ctcgggccgt tcttcttcca cctgtacggg ctgtgcatcg ccgtcggggt gctggccgcg
      601 ttctggctgg ccaaccggcg ctgggtccag gccggcggcc agccgggcga gctcgagcgc
      661 ccggccatct gggcggtcat cgccgggttc ctgggcggcc gcctcggcta cgtcatcacc
      721 cacaccggcg acttccgcga caacccgctg gaggtgttcg ccatctggca gggcgggctg
      781 gccctgtacg gcgggctgac cgtcggcatc ctcaccgggc tgtgggtggc ccgccggcgc
      841 ggcctgcccg tgctcaaggc cctggacgcc gccatcccgg ccatccccct ggcccaggcc
      901 atcgggcgct ggggcaacta cttcaaccag gagctgttcg gcacccccac gaccgtcccc
      961 tgggcgctgg aggtcgaccc cgagcaccgg cccgaccgct acgcctcctt cgccaccttc
     1021 caccccacct tcctgtacga gtccatctac aacctgggcg tggtggtcgt cctgctgcgg
     1081 gtgtcggcca cccggcggct ccgccccggc agcctgtcgc tgctgtacct ggtcctgtac
     1141 ggcgccgggc ggttcgggct ggagctgctg cgcaccgaca ccacctaccg gctgggtggc
     1201 ctgtcccgca acgcctggat ctcgctggcc ctggtcgtgg gcgggtcggt ctggctgtgg
     1261 ctgcgggagc gccgccggcc ggccgccgaa gccgacgacg acgccgagga gcggcaatgg
     1321 tcatcctgaa gtcccgggac gagatcgcca ccatgcgccg ggccgggcgg gtggtggccc
     1381 ggaccctgga gcgggtcgtg gccgcggtcc ggcccggggt gagcctgcgc gagctggacg
     1441 acctcggggc ccggacgatc gccgccgagg gcgccaccgc ctcgttcctc ggctaccacc
     1501 cgcggttcgc gccgaccccg ttcccggcca gcctgtgcct gtcggtcaac gaggtgatcg
     1561 tccacggcat ccccgaccgg cgccggctgc gggccgggga cgtgctctcg atcgactgcg
     1621 gcgcggtggt ggacggctac cacgccgacg cggccgtgac cgtgcccgtc ggcgagatcg
     1681 acccggccgc ggcccgcctc ctggaggcca ccgagcgggc cctgtgggcc gggatcgagc
     1741 aggcccgggt gggcaaccgg ctgtcggaca tcagccacgc cgtcggccag gtcgcccgcg
     1801 ccgccggcta cggcatcctg gccgaccacg gcggccacgg cgtcggccag ctgctccacg
     1861 aggacccgtc ggtcgacaac gacggccgcc ccggccgggg cccacgcctg cgcgacggcc
     1921 tcgtcctggc catcgagccg atgttcgtcg acggcgaccc ggcctacgtc caccgccccg
     1981 acggctgggc ggtggcgacc cgcgacggct cccggtcggc ccacttcgag cacaccgtgg
     2041 ccgtcctcga cgagggcccg gagccgctga ccgtccccta gccctcgggc ggcgccctgg
     2101 ggctacgatc aggggagcat gcggctcctc gagcgggaac cggcgctgga ggccctcggc
     2161 ggctggctgg ccgaggcggg ggccggccgc ggccggctgg tcctggtcgc cggcgaggcc
     2221 ggcgtcggca agaccgccct ggtcgaggag ttcacggtcc ggcagcggcc
//
LOCUS       DASSWR010000068         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_567754,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000068 DASSWR010000000
VERSION     DASSWR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567754"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1189
                     /locus_tag="VFO47_01160"
     CDS             125..1189
                     /locus_tag="VFO47_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787339.1"
                     /translation="MADSEAYLEALPDAVVVVDAEGRVAYANAAAERLARAGRDELAG
                     RPLDDAVPLRDGAGNPWWTCTERLRRLPGVRRVPVRELHLVAGGEPVPVDLTASFQRD
                     AAGRVVQAVCVLRDASARNRGDSQRGELISTLAHELRSPLTSVKGFTSTLLHRWERFS
                     DEQKRQMLATVNMDADRVTRLIGELLDVSRIDAGRLELRRKEFDLAAMAEGIVGRFDL
                     QHDGHTFVITFPEDFPAVYADPDKIEQVLTNLVENAVKYSDGGPVTVTGRATETEVEV
                     AVHDQGVGVPTGELPLIFTKFYRRAGSGAPSGTGLGLFIARGLVEAHDGRIWADSAPG
                     QGSTLRFRLPRGGLELAGIT"
     gene            1269..2318
                     /gene="pheS"
                     /locus_tag="VFO47_01165"
     CDS             1269..2318
                     /gene="pheS"
                     /locus_tag="VFO47_01165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET8787340.1"
                     /translation="MPTPDLDAVVAAGRQAIAAATDLEELREAEQAHLAKRSALGEVQ
                     RSLGGLDEAARRDLGRRVNQARTTLQAELAARRAELEAERDAALLEAERVDVTLPGRV
                     PPRGAIHPVTRTIDEIVDIFIGLGYRVAEGPEVETDWYNFQALNIPADHPARSMQDSL
                     YLAGDDGRETGELVLRTQTSPVQIRTMQAGPPPVYVVAPGRCYRADTPDATHVPVFNQ
                     IEVLAVDRGLTMADMKGTLLAFARAYFGEERQIRLRPSYFPFVEPGAEVDVSCFICGG
                     TGAGCRTCRGEGWIELLGAGMVHPNVLRAGGYDPDEVSGFAAGTGIERPFMLRSGLAD
                     MRTLTDNDVRWLTSV"
     gene            2335..>3085
                     /locus_tag="VFO47_01170"
     CDS             2335..>3085
                     /locus_tag="VFO47_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET8787341.1"
                     /translation="MRVPLSWLASHVDVDVPAATLADKLTFAGIEIETVHRVGDDLAG
                     ILTARVLEVSPHPDADRLVLVRIEAGGEDRSVVCGARNFAPGDVVPWAAPGAKLPGGV
                     EIGRRKVRGALSDGMLASARELGVFDDHSGILVLPPDTPVGADVVEAVDLRDTVLDVK
                     TAPNRGDILSMRGIAREVALLLGKELKPLDLSVPETASAAGSLASVAVDDEEGCPLYA
                     ARVIQGLDASRPSPLRIARRLYLYGQRPLGAV"
BASE COUNT          405 a         1218 c         1077 g          385 t
ORIGIN      
        1 cgagagcctc aacctggccg ccgccgcggc cgtcctgctg tacgagacct cgcgccggca
       61 ggcggcccgg aggggtccgg ggaaccccga gggggtgccc cggtgaatcg gggggaggcg
      121 gccgttggcc gatagcgagg cctatctgga ggcgttgccc gacgccgtcg tggtggtcga
      181 cgccgagggc cgggtcgcct acgccaacgc ggccgccgag cgcctggcca gggccggccg
      241 ggacgagctg gccggccgcc ccctcgacga cgccgtgccc ctgcgcgacg gcgccggcaa
      301 cccctggtgg acctgcacgg agcggctgcg ccgcctcccc ggggtccggc gggtcccggt
      361 gcgcgagctc cacctggtcg ccggcggcga gccggtcccg gtcgacctca cggccagctt
      421 ccagcgcgac gccgccggcc gggtcgtcca ggccgtctgc gtgctccggg acgcctcggc
      481 ccgcaaccgg ggcgacagcc agcggggcga gctcatctcc accctcgccc acgagctgcg
      541 ctcccccctg accagcgtca agggcttcac ctcgaccctg ctgcaccgct gggagcgctt
      601 cagcgacgag cagaagcgcc agatgctggc caccgtcaac atggacgccg accgggtcac
      661 ccggctcatc ggggagctgc tggacgtctc ccgcatcgac gccggccgcc tggagctgcg
      721 gcgcaaggag ttcgacctgg ccgccatggc cgagggcatc gtcggccgct tcgacctcca
      781 gcatgacggc cacaccttcg ttatcacgtt cccggaggac ttcccagccg tctatgctga
      841 cccggacaag atcgagcagg tgctgaccaa cctggtggag aacgcggtga agtactccga
      901 cggcgggccg gtgacggtga ccgggcgggc gaccgaaacc gaggtcgagg tcgccgtgca
      961 cgaccagggg gttggcgtcc ccaccgggga gctgccgctg atcttcacca agttctaccg
     1021 gcgggccggc tccggggcgc ccagcggcac cgggctcggc ctgttcatcg cccgcggcct
     1081 ggtcgaggcc cacgacggcc gcatctgggc cgactcggcc cccggccagg gcagcaccct
     1141 gcgatttcgc ctgccccgcg ggggcctgga gctggccggc atcacctagc ccggcacccg
     1201 gcccgagcgg ggcgaccgcg aagcggacgt gacgtttgcg ctgtgccttc ccgtgaggag
     1261 tcccgaccat gcccacccca gacctggacg ccgtcgtcgc cgccgggcgg caggcgatcg
     1321 ccgccgccac cgacctcgag gagctccgcg aggccgagca ggcccacctg gccaagcgct
     1381 cggccctcgg ggaggtccag cgctccctgg gcggcctcga cgaggccgcc cgccgcgacc
     1441 tgggccgccg ggtcaaccag gccaggacca ccctccaggc cgagctggcc gcccgccggg
     1501 ccgagctgga ggccgagcgc gacgccgccc tgctcgaggc cgagcgggtc gacgtcaccc
     1561 tgcccggccg ggtccccccg cgcggggcca tccacccggt gacccgcacc atcgacgaga
     1621 tcgtcgacat cttcatcggt ctcggctacc gggtcgccga aggccccgag gtcgagaccg
     1681 actggtacaa cttccaggcc ctcaacatcc cagccgacca cccggcccgc tccatgcagg
     1741 acagcctgta cctggccggg gacgacgggc gcgagacggg ggagctggtg ctgcgcaccc
     1801 agacctcgcc ggtccagatc cgcaccatgc aggccggccc gccgccggtc tacgtcgtcg
     1861 cccccggccg ctgctaccga gccgacaccc cggacgcgac gcacgtgccg gtgttcaacc
     1921 agatcgaggt cctggccgtc gaccgcggcc tgaccatggc cgacatgaag gggacgctgc
     1981 tcgccttcgc ccgcgcctac ttcggcgagg agcgccagat ccgcctgcgc ccctcctact
     2041 tcccgttcgt cgagccgggg gccgaggtcg acgtctcctg cttcatctgc ggcggcaccg
     2101 gggccggctg ccgcacctgc cgcggcgagg gctggatcga gctgctgggc gccggcatgg
     2161 tccaccccaa cgtgctccgg gccggcgggt acgaccccga cgaggtctcc ggcttcgccg
     2221 ccgggaccgg catcgagcgg cccttcatgc tccgctccgg cctggccgac atgcgcaccc
     2281 tgaccgacaa cgacgtccgc tggctgacca gcgtctagag aggaggagca ggcgatgcgt
     2341 gtcccactct cctggctggc cagccacgtg gacgtcgacg tcccggcggc caccctggcc
     2401 gacaagctga ccttcgccgg catcgagatc gagaccgtcc accgggtcgg ggacgacctg
     2461 gccggcatcc tcaccgcccg ggtcctggag gtgagcccgc accccgacgc cgaccgcctc
     2521 gtcctggtcc gcatcgaggc cggcggcgag gaccgctcgg tggtctgcgg cgcccgcaac
     2581 ttcgcccccg gcgacgtcgt gccctgggcg gcccccgggg ccaagctgcc cggaggggtc
     2641 gagatcggcc gccgcaaggt ccgcggcgcc ctctccgacg gcatgctcgc ctccgcccgc
     2701 gagctgggcg tgttcgacga ccactccggg atcctggtcc tgccgcccga cacgcccgtc
     2761 ggggccgacg tggtcgaggc ggtcgacctg cgcgacaccg tgctcgacgt caagaccgcc
     2821 cccaaccgcg gcgacatcct ctccatgcgc ggcatcgccc gcgaggtcgc gctgctgctc
     2881 ggcaaggagc tcaagccgct cgacctgtcg gtgcccgaga ccgccagcgc cgccggctcg
     2941 ctcgcgtcgg tcgccgtcga cgacgaagag ggctgcccgc tgtacgccgc ccgggtcatc
     3001 cagggcctgg acgcctcccg gcccagcccg ctgcggatcg ccaggcgcct gtacctgtac
     3061 ggccagcggc cgctgggagc ggtgg
//
LOCUS       DASSWR010000069         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1132589, whole genome shotgun sequence.
ACCESSION   DASSWR010000069 DASSWR010000000
VERSION     DASSWR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132589"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..937)
                     /locus_tag="VFO47_01175"
     CDS             complement(80..937)
                     /locus_tag="VFO47_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787342.1"
                     /translation="MFGAGSLWVGAWRDGQVVRVDPHSNRVVARFPAGGPNPAGLAVG
                     AGTIWVVHPDTDEVVRLDSGTGRVVARIKLGPLPFQFGAGDRRFLPSLVAVGAGAGWV
                     GTGRGAVARIDLASNRVVAVIKLRPGSPAGIAVAGGSVWVAAGGDGVARIEAATNRLL
                     GAIRLDLYAERVAVGGGALWVGGPTTDPIVEIAGGVARIDAATGRVREIVPTGLPIGL
                     AAAAGAVLITEPDTVGGALECIDPERLPAAMTLQPALGELAVGGGAVWVADRRGSLVY
                     RLDPDRLPC"
BASE COUNT          298 a          793 c          734 g          282 t
ORIGIN      
        1 gctgttccgt ctcctggagc ggcaagcccg gcctgggtca cggcgtgacg ggcaaggctt
       61 gtgagcgccg aggccacgct cagcacggga ggcggtcggg atctagtcga tacaccaacg
      121 agccgcggcg gtcggcgacc cagacggcgc cgccgccaac ggccagctcg cccagggcgg
      181 gctgcagggt catggctgcg gggagccgct ctgggtcgat gcactccagc gccccgccaa
      241 cggtgtccgg ctcggtgatc aagacggcac cggcggcggc ggccaggccg atcgggaggc
      301 cagtggggac gatctcccgg acccggccgg tggcggcgtc gatccgggcc accccaccag
      361 cgatctcaac aatcgggtcg gtggttgggc caccgaccca gagcgccccg ccgccgaccg
      421 ccacccgctc ggcgtagagg tcgagccgaa tggccccaag cagccggttg gtggccgcct
      481 cgatccgggc cacgccatcg ccgccggcag cgacccagac gctcccgccg gcgacggcaa
      541 tgcccgcggg actcccaggg cgaagcttga tcaccgcgac cacgcggttg ctggccagat
      601 cgatgcgggc caccgcaccg cgaccggtac caacccatcc ggcacccgcg ccgactgcca
      661 ccagcgacgg caggaagcgg cgatcaccgg ccccgaactg gaacggcagc ggaccgagct
      721 tgatcctggc aacgactcgg ccggtcccgg agtcaaggcg caccacctca tcggtgtcgg
      781 gatgcaccac ccagatcgtc cctgccccga ccgccaggcc agccggattc ggccccccgg
      841 ctgggaagcg ggcgacgact cggttggagt gcggatcgac ccgcacgacc tggccgtctc
      901 gccacgcgcc gacccatagg ctgccggcgc cgaacaccag cgggccaccg ccatggccga
      961 cctcgatctg cgctgtcgcc cagcgatccg gcagggggag ctgctgggaa ggcgccggtg
     1021 tcctagtggg cgtcggcggc gaccgtgacc caggcgcttg ggggctggtg atgcgcccca
     1081 ccacggcgag cactgcgacc gccccgagca cggcggcacc gacgcgcagc acctcacgct
     1141 gtgaggccat agggtcgacc tcgccggttc ccagcccaca gcgccatcgg cgacacgcca
     1201 tccagaggcc gagcggccca tctgcttcct acaaggccat cctacgacga gcggctgccc
     1261 ctctcgaagc ttcaccctgg ccgcgccgcc gccggagggg ggtccgggga cccctccccc
     1321 cgggatactg gccaccaggc tcgcccgggg agcggtggtg cagctgcggc accgctgccc
     1381 agagtcatgc gcgatgggac ggccgggctc gtcggctgct ggatcaggcg gccgccgaag
     1441 gcggcaccac cagccggcga gttcggccgc ctacctcgat cgcgcttgac cgggaccccg
     1501 gccagccgcc ggccgctggc cacagtcggt agcgggtgag ggacgcgcct tcggcgcgtg
     1561 ctgcttcgct ggcaagctcc gcagccctgc cggtcccggc ccggtccccc caccgtggcc
     1621 caggccctcg acccctacgt cattccctac gcgcaggcat taccgaggtc gtgtcggcag
     1681 cggcagggct gctcctggtg gccacggggt tgtgggcctt cgccgctccc cggtcgttct
     1741 tcgaatcggt ggccacctat ccacggttca acaagcactt gctccacgac atcggggcgt
     1801 tcaactcggc ctgggagcca ccttgctcct ggcgttggcc ggcgtgggtt tgggggccac
     1861 ggcccatgcg ctggcccact ggctggaccg ggaactgggc ggctccgcca ctgatccctg
     1921 gtacctgagc gccttcgtcc ctggtgctcc tggcagcggc tgcctgggga tggaatgagc
     1981 ttcggcgatg acaatccctg tgttcggcgt cctccgccgt tcggcgcttg gatggcgtga
     2041 cgagcacctc ggcggccgga ccgcagcgac tgaggcctcc gagccgctgc gcttcgacgg
     2101 gtggctg
//
LOCUS       DASSWR010000070         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_285189,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000070 DASSWR010000000
VERSION     DASSWR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285189"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="pyk"
                     /locus_tag="VFO47_01180"
     CDS             complement(<1..1206)
                     /gene="pyk"
                     /locus_tag="VFO47_01180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET8787343.1"
                     /translation="MKSVERTKVVATLGPASSSREILGGMVEAGLDVVRLNFSHGERA
                     DHVARFELVRAVAKELDHNLAVLVDLQGPKIRVGVVGDDGVRLDRGEEVTLVSGTETC
                     AEPDIPVVYPALATDVHQGDRILLDDGAIGLRVEDVEGERVRCQVERGGVVKSRKGVN
                     LPGVAVSAASLTAKDRADVATAVEFGADYVALSFVRKPEDVAEAKQAIAEHGGDIPVV
                     AKLERPEAIDVLDEILEVADAVMVARGDLGVELAVEQVPPIQKHIIARANSLGVPVIT
                     ATQMLESMVASPRPTRAEASDVANAIFDGTDAVMLSQETAVGQYPVEAVATMSRIARE
                     AEATPYLTAPPPPAVGGLDVPATVCRAAVQVAADLGARAIVAFTESGATARYVSRFRP
                     RTPIIGLTTS"
     gene            1311..2225
                     /locus_tag="VFO47_01185"
     CDS             1311..2225
                     /locus_tag="VFO47_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET8787344.1"
                     /translation="MRFDDDSQLDSSQVQDARGRGGFSGFPRRGGVAIGGGAGLLGVI
                     VFILLQLLGGGTATSGALPGIDQIQGEQSNLAEECKTGADANTKEDCRVVAVVNSLQD
                     YWTAEFQRRGGQYQKAPTTLFSGGVQTGCGAASSEVGPFYCPRDGTVYIDLSFYRDLQ
                     TKFGAQGGPFAQAYVLAHEYGHHVQNLTGTMEQAGSSRATGPQSAAVRLELQADCFAG
                     LWANHATTEPGPDGGDPLVERITEQDIADGLDAAAAIGDDRIQERFQGQSNPETWTHG
                     SAEQRQRWFTTGYRTGDYAQCNTFRGSV"
BASE COUNT          317 a          850 c          802 g          290 t
ORIGIN      
        1 ggaggtggtc aggccgatga tcggggtccg gggccggaac cgggagacgt agcgggcggt
       61 cgcccccgac tcggtgaagg ccacgatcgc ccgggccccc aggtcggcgg cgacctgcac
      121 ggccgcccgg cagacggtgg cgggcacgtc cagcccgccg acggccgggg gcggcggggc
      181 cgtgaggtac ggggtcgcct cggcctcgcg ggcgatgcgg ctcatggtgg ccaccgcctc
      241 gaccgggtac tggccgacgg ccgtctcctg ggagagcatc accgcgtcgg tgccgtcgaa
      301 gatggcgttg gccacgtcgc tggcctcggc ccgggtcggg cgggggctgg ccaccatcga
      361 ctcgagcatc tgggtggcgg tgatcaccgg caccccgagc gagttggccc tggcgatgat
      421 gtgcttctgg atgggcggga cctgctcgac ggccagctcg accccgaggt cgccccgggc
      481 gaccatgacc gcgtcggcga cctccaggat ctcgtccagg acgtcgatcg cctccggccg
      541 ctccagcttg gccaccaccg ggatgtcgcc gccgtgctcg gcgatggcct gcttggcctc
      601 ggccacgtcc tcgggcttgc gcacgaacga cagggccacg tagtcggcgc cgaactcgac
      661 cgcggtggcc acgtcggcgc ggtccttggc ggtcagcgaa gccgccgaga cggccactcc
      721 gggcaggttg acgcccttgc gggacttgac caccccgccc cgctcgacct ggcagcggac
      781 ccgctcgccc tcgacgtcct cgacccggag gccgatggcg ccgtcgtcga gcaggatccg
      841 gtcgccctgg tggacgtcgg tggccagcgc cgggtagacc accgggatgt cgggctcggc
      901 gcaggtctcg gtgccggaga ccagcgtgac ctcctcgccg cggtcgagcc ggaccccgtc
      961 gtcgccgacc accccgaccc ggatcttggg cccctggagg tcgaccagca cggccaggtt
     1021 gtggtcgagc tccttggcca cggcccggac gagctcgaac cgggccacgt ggtcggcccg
     1081 ctcgccgtgg gagaagttca gccgcaccac gtcgaggccg gcctcgacca tcccgccgag
     1141 gatctcgcgg ctgctcgagg ccgggcccag ggtggccacg accttggtac gctcgacgct
     1201 cttcatcgtg ccggcagttt ggcacgactc gcggcccgtc gcgacggttg gcgggtgacc
     1261 ggctcgggta ttcctgacgg caggcggcga ccccagagag ggcaggcagg atgcgcttcg
     1321 acgacgacag ccagctcgac agctcacagg tccaggacgc ccgcggccgg ggcggcttca
     1381 gcggcttccc gcgccgcggc ggggtggcca tcggcggcgg cgccgggctc ctcggcgtga
     1441 tcgtgttcat cctgctccag ctgctcggcg ggggcaccgc cacctccggc gccctccccg
     1501 gcatcgacca gatccagggc gagcagagca acctggccga ggagtgcaag accggggcgg
     1561 acgccaacac caaggaggac tgccgggtcg tggccgtggt caacagcctc caggactact
     1621 ggacggccga gttccagcgc cgcgggggcc agtaccagaa ggccccgacg acgctgttct
     1681 cgggcggcgt ccagaccggc tgcggcgcgg ccagctccga ggtcggcccc ttctactgcc
     1741 caagggacgg caccgtctac atcgacctca gcttctaccg ggacctgcag accaagttcg
     1801 gcgcccaggg cggccccttc gcccaggcct acgtgctggc ccacgagtac ggccaccacg
     1861 tccagaacct caccggcacg atggagcagg ccggcagctc ccgggccacc ggcccccaga
     1921 gcgcggccgt ccgcctcgag ctccaggccg actgcttcgc cggcctgtgg gccaaccacg
     1981 ccaccaccga gcccggcccc gacggcggcg accccctggt cgagcggatc accgagcagg
     2041 acatcgccga cggcctcgac gccgccgccg ccatcggcga cgaccgcatc caggaacggt
     2101 tccagggcca gtccaacccc gagacctgga cccacggctc cgccgagcag cgccagcgct
     2161 ggttcaccac cggctaccgc accggcgact acgcccagtg caacaccttc cgcgggtcgg
     2221 tctaaagcct gtcgaacaac ctcgtggtga ggcgtccgg
//
LOCUS       DASSWR010000071         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_963521,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000071 DASSWR010000000
VERSION     DASSWR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963521"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1312)
                     /locus_tag="VFO47_01190"
     CDS             complement(164..1312)
                     /locus_tag="VFO47_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8787345.1"
                     /translation="MADELLAYRDRLPTLARGPYFAAHTLGPMPDTVPDALARFAAEW
                     AGQGVVAWYGWLDQIRATAGLLEGLFGAPAGSVALGPNVSVLAGQVLSCFDWSGERSR
                     LITTDLEFPTCDYLYRAQETLGAKVEVVPSRDLAVDLDRLLEAIDTRTALVAVTHVAF
                     RSSALLDAAAVAARAHEVGALVMLDTYQSAGTVPIDVAALDVDLMVGGSVKWLLGGPG
                     TGYLYARPEVAAGLAPWLVGWFGHEAPFAFRPSPIAYAEGAGRFVTGTPNVAAHVMAA
                     EGYRMVAEAGLDAIRAKSRRQVARLLDGFQGQGAVVRGPADPAARGGSVVVDFDGAEQ
                     VTQGLIARGYTCDYRPGAGLRLGPHLYTTDDECDAVVAEVAALRGRSV"
     gene            complement(1479..2387)
                     /locus_tag="VFO47_01195"
     CDS             complement(1479..2387)
                     /locus_tag="VFO47_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET8787346.1"
                     /translation="MEVTVVGVPFNSAGLTDGVARAPAALRRAGLVSGLARRLDVADA
                     GDVGFDPPAPERSPTSGLLAEPALVSMVAGTRQAVAAVHQAGRFPLVVGGDCPVMLGA
                     LAATRDRHGGVGLLMLDGHEDAYPPRRSPTGEAADSELALALGLAEGLPDGLAALVPL
                     LAPGQVGLLGPRDDATIAREGVASLRGVVPLWSDVELAVRGAAEVAAQAARDVAAAAP
                     AWWLHVDLDVLATAELAAVDYPQPGGLRWWQLRQMTVAALAVPGCAGWTVAIYNPDLD
                     PDGGQAARIVEYVAGAAARLAEPPDA"
     gene            complement(2406..3191)
                     /locus_tag="VFO47_01200"
     CDS             complement(2406..3191)
                     /locus_tag="VFO47_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787347.1"
                     /translation="MIQGRVPDRAARHRYPLAGAVRLRVRELARRPVVVAAFSLQAMA
                     VVAAELLAAVAAEGLAGAGDDLVLGGLRVTAVPGGLVFGVLVAAVVGAEFGWATERAL
                     LARDPRRLRFAGYQLAVAAALALAWWAVQSLLAVTVAALLQAGGGIVSGTPWNAPAHL
                     AALAAALAATLLYGLLGAACALVLRGALAGVVALLAYGLLGELVLAPQWAPATGWTIY
                     TSAARLTGQGTTPMPRAALVVTLAFVVALAGCLALYARRQVRD"
     gene            complement(3188..4180)
                     /locus_tag="VFO47_01205"
     CDS             complement(3188..4180)
                     /locus_tag="VFO47_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787348.1"
                     /translation="MVQCLRNHLRARRPATAAVELSGVYRRFGRTMVLAGLDLRVDEG
                     ELYGLIGPNGAGKTTALRVALGLLQADAGEVRLLGRDPRSAAGARPRRVGSLIEAPAL
                     SPRLTGREYLVLIARWAHAPDAEVDRVVGLLGLGRMTDRQTATYSTGMRQVLATAAAL
                     VGPPDLLILDEPTVGLDPGHRRRVNDLLLDHVARGGTVLLSSHQLDDAERLCTRVGLM
                     AAGRLVEEGPPTELGPAAGRFELTVSDPEAALSALRAMRLICWRDNGKVLVETGAPYQ
                     VGRPDGSAIVRVLANAGVYPSDLRRSPTLELAYAAVTSSLAPEPPRPPIAEASP"
     gene            4200..>4544
                     /locus_tag="VFO47_01210"
     CDS             4200..>4544
                     /locus_tag="VFO47_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787349.1"
                     /translation="MTSTARVTRAGSRQEAVLAALAAGILGEAFDPAVPGRMLEILGR
                     TASRRDREQLLGVLRALGSRAGALALTGRPAPLPGLDPAAAEAVVRRWAAARLPLRRR
                     LAAANTPITLLAA"
BASE COUNT          579 a         1733 c         1780 g          452 t
ORIGIN      
        1 accgccacgc cccccgcttt gtgcccaccc tgggcgtcaa ggagttcctc ccccgccaca
       61 cgggcacccg caggaagggc gacgccgcca ccgccgccaa ggccgaggac cgcacccggg
      121 tccgcaccga gccgtccaag agccagaaga agctggccgg gctctagacg cttcggccgc
      181 ggagcgcggc gacctcggcc accacggcgt cgcactcgtc gtcggtggtg tacaggtggg
      241 ggccgaggcg caggcccgcc ccggggcggt agtcgcaggt gtagccgcgg gcgatcaggc
      301 cctgggtgac ctgctcggcg ccgtcgaagt cgacgaccac gctgccgccc cgggccgccg
      361 ggtcggcggg gccgcggacg accgcgccct ggccctggaa gccgtccagc aggcgcgcga
      421 cctggcgccg tgacttggcc cggatggcgt ccaggccggc ctcggcgacc atccggtagc
      481 cctcggcggc catcacgtgg gcggcgacgt tgggggtgcc ggtgacgaac cggcccgccc
      541 cctcggcgta ggcgatcggg ctgggccgga acgcgaacgg cgcctcgtgg ccgaaccagc
      601 cgaccagcca cggggccagc ccggcggcca cctccggccg ggcgtacagg tagccggtgc
      661 cggggccgcc gagcagccac ttgaccgagc ccccgaccat caggtcgacg tccagggcgg
      721 cgacgtcgat ggggacggtc ccggccgact ggtaggtgtc gagcatgacc agcgccccca
      781 cctcgtgggc gcgggcggcc acggcggccg cgtccagcag ggccgaggac cggaaggcca
      841 cgtgggtgac ggcgaccagg gccgtccggg tgtcgatcgc ctcgagcagc cggtccaggt
      901 ccacggccag gtcgcgggag ggcacgacct cgaccttggc ccccagcgtc tcctgggccc
      961 ggtacaggta gtcgcaggtg gggaactcga ggtcggtggt gatcagccgg ctccgctcgc
     1021 ccgaccagtc gaagcacgac agcacctgcc cggccagcac cgacacgttg ggcccgaggg
     1081 ccaccgaccc cgccggcgcc ccgaacagcc cctccagcag cccggccgtc gcccggatct
     1141 ggtccagcca cccgtaccag gccaccaccc cctggccggc ccactcggcc gcgaaccggg
     1201 ccagcgcgtc cggcaccgtg tccggcatcg gcccgagcgt gtgggcggcg aagtaggggc
     1261 cccgcgccag ggtcggcaat cggtcacggt aggcgagcag ctcgtcggcc acggcaaccc
     1321 tccttcagaa cagcggtcga gtatccggcg ccgcataccg cgctgagcca tccgtgcagc
     1381 gttgcagctt gcgcgccgct cccggcggcc agccaattac ctcaaacccg gatgcttggc
     1441 cggtgttctg gtggtcggga ccggcccggc tcatgctgct aggcgtctgg cggttcggca
     1501 agcctggccg cggccccggc cacgtactcg acgatccggg cggcctgccc gccgtccggg
     1561 tcgaggtcgg ggttgtagat cgccaccgtc cagccggcgc atccgggcac cgccagcgcg
     1621 gccacggtca tctggcggag ctgccaccag cgcaggccgc ccggctgcgg gtagtcgacg
     1681 gcggccagct cggcggtggc cagcacatcc aggtcgacat ggagccacca cgccggggcg
     1741 gccgcggcca cgtcccgggc cgcctgggcg gcgacctcgg ccgctccccg gacggccagc
     1801 tcgacgtccg accacagcgg gaccacgccc cgcagcgagg cgacaccctc gcgggcgatg
     1861 gtggcgtcgt cccgcggccc gagcagcccg acctggccgg gggcgagcag ggggaccagc
     1921 gcggccagcc cgtcgggcag cccctcggcc aggccgaggg ccagcgccag ctcggagtcg
     1981 gcggcctcgc cggtggggga gcgccgcggc gggtaggcgt cctcgtggcc gtccagcatc
     2041 agcagcccga ccccgccgtg gcggtcgcgg gtggcggcca gcgcgcccag catgaccggg
     2101 cagtcaccgc cgacgaccag ggggaaccgg ccggcctggt ggacggcggc caccgcctgg
     2161 cgggtgccgg cgaccatcga gaccagcgcc ggctcggcca gcaggccgga ggtggggctg
     2221 cgctcgggcg ccggcgggtc gaagccgacg tcgccggcgt cggccacgtc cagccgcctg
     2281 gccaggccgg agaccagccc ggcccggcgc agggcggccg gggcccgggc cacgccgtcg
     2341 gtcagcccgg ccgagttgaa cggcaccccc acgacggtga cctccatgac ctggctcccc
     2401 tcgggtcagt cgcgaacctg gcggcgggcg tagagggcga ggcagccggc cagggcgacc
     2461 acgaaggcga gggtgacgac cagggcggcg cggggcatgg gggtggtgcc ctggccggtg
     2521 aggcgggcgg cggaggtgta gatggtccag ccggtggccg gggcccactg gggggcgagg
     2581 acgagctcgc cgaggaggcc gtaggcgagc agggcgacca cgccggccag ggcgccccgc
     2641 aggaccaggg cgcaggcggc cccgaggagg ccgtacagca gggtggcggc cagggcggcg
     2701 gccagggcgg ccaggtgggc gggggcgttc cagggcgtcc cggagacgat gccgccgccg
     2761 gcctggagca gggccgcgac cgtgaccgcg agcaggctct gcaccgccca ccaggccagg
     2821 gccagggcgg cggccacggc cagctggtag ccggcgaagc ggagccggcg cgggtcgcgg
     2881 gccagcaggg cccgctcggt cgcccagccg aactcggcgc cgaccacggc cgcgaccagc
     2941 accccgaaga ccagcccgcc gggcacggcc gtgacccgca ggccgcccag gacgaggtcg
     3001 tcgccggccc cggccagccc ctcggcggcc acggcggcca gcagctcggc cgcgaccacg
     3061 gccatcgcct ggagcgagaa cgccgccacc accaccggcc gccgggccag ctcccggacc
     3121 cgcagccgca ccgccccggc cagcgggtac cggtgcctgg cggcccggtc cgggaccctc
     3181 ccctggatca aggggacgcc tccgcgatcg gggggcgagg cggctcgggg gccaggctgc
     3241 tggtcacggc ggcgtaggcc agctcgaggg tgggggagcg gcgcaggtcg gaggggtaga
     3301 ccccggcgtt ggccaggacc cggacgatgg cgctgccgtc gggccggccc acctggtagg
     3361 gggcgcccgt ctcgaccagc accttgccgt tgtcgcgcca gcagatcagg cgcatggccc
     3421 gcagcgccga cagggcggcc tcggggtcgc tgacggtgag ctcgaagcgg ccggcggccg
     3481 ggcccagctc ggtcggcggg ccctcctcga ccagccggcc ggcggccatc agcccgaccc
     3541 gggtgcagag ccgctcggcg tcgtcgagct ggtggctgga cagcagcacc gtcccgcccc
     3601 gggcgacgtg gtcgagcagc aggtcgttga cccggcggcg gtggcccggg tcgagcccga
     3661 ccgtcggctc gtcaaggatc agcaggtcgg gcggcccgac cagggcggcc gcggtggcca
     3721 gcacctggcg catgccggtc gagtaggtgg cggtctgccg gtcggtcatc cggcccagcc
     3781 cgaggaggcc gaccacccgg tcgacctcgg cgtcgggggc gtgggcccag cgggcgatca
     3841 ggaccaggta ctcgcggccg gtcagccgcg gcgacagggc cggggcctcg atcaggctgc
     3901 cgacccgccg gggccgcgcc ccggccgccg accgcgggtc gcggccgagc agccgcacct
     3961 cgccggcgtc ggcctggagc agcccaaggg cgacccgaag ggcggtcgtc ttgccggcgc
     4021 cgttgggtcc gatcagcccg tagagctcgc cctcgtcgac gcgcaggtcg aggccggcga
     4081 gcaccatggt gcgcccgaag cgccggtaca cgccgctgag ctcgaccgcc gccgtcgccg
     4141 gcctccttgc tcgcagatgg ttgcgcaggc attgaaccac taggtgtaca aaccttgacg
     4201 tgacgtccac tgcgcgggtt acgagggccg ggtcccggca ggaggccgtc ctggccgcgc
     4261 tggccgccgg gatcctcggc gaggccttcg acccggccgt gcccggccgg atgctggaga
     4321 tcctggggcg gacggcgagc cggcgggacc gcgagcagct cctcggcgtg ctgcgggcgc
     4381 tcgggtcgcg ggccggcgcc ctcgccctca ccggccggcc ggcgccgctg cccgggctcg
     4441 acccggccgc ggccgaggcg gtggtgcggc gctgggcggc ggcccggctc cccctgcggc
     4501 ggcggctggc cgccgccaac accccgatca ccctgctggc cgcc
//
LOCUS       DASSWR010000072         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_144176,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000072 DASSWR010000000
VERSION     DASSWR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144176"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFO47_01215"
     CDS             <1..630
                     /locus_tag="VFO47_01215"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HET8787350.1"
                     /translation="RVFEDRVTEELQREYDLGRRPVVQVRDFPFLPHLATGRFSTIDV
                     AATDASAKGINAAQLQLTLHGVEVPGEVLLGEPGRVTVERADGTIELTEAEVNRLLAD
                     RLQGGALAIEADGVEVRTQVPFLGQTLDALVAGKLGARDGQVTFTAEEVRLGDQGPLD
                     PAQTDLLASRFTFQVPLPELPAGVRLERVETRPGTVVLSGRAASVGVAA"
     gene            1017..1973
                     /locus_tag="VFO47_01220"
     CDS             1017..1973
                     /locus_tag="VFO47_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787351.1"
                     /translation="MAALTSIVDRPDLATVALAGNDVLRYLHAVCTQHTLDLEPGDAT
                     QALLLSPKGKVEFAFRLAVLDEGVLLDTEAEAAGALAERLARFVFRHDVTVGQPVVGA
                     ASLLGPGAGDLLAAAGLPVPAPGRAGVGGPDLVVHHTPAGFDLVGPGALVVFAALERA
                     GAERRPAELWEQVRVELGLPRAGRELTDDVLAEEAGLLGSHVHLDKGCYPGQETVARV
                     HNLGQVQRRLAGLRFHPHGDGGQGRSLDTEPAGAGDGDGLPTPRTDLVADDGRRAGQL
                     RSVVDHPRLGPIGLAYVRRVVDSGRLVRAGDRVATVVDLPFH"
BASE COUNT          264 a          764 c          756 g          284 t
ORIGIN      
        1 cgggtgttcg aggaccgggt caccgaggag ctgcagcgcg agtacgacct cggccggcgg
       61 ccggtggtgc aggtgcgcga cttcccgttc cttccccacc tggcgaccgg gcggttctcc
      121 accatcgacg tggccgccac cgacgcctcg gccaagggaa tcaacgccgc ccagctccag
      181 ctcaccctcc acggcgtcga ggtgccgggg gaggtgctgc tgggcgagcc cggccgggtc
      241 accgttgagc gcgccgacgg caccatcgag ctgaccgagg ccgaggtcaa ccggctgctg
      301 gccgaccgcc tccagggcgg cgcgctggcc atcgaggcgg acggggtcga ggtccggacc
      361 caggtcccgt tcctcggcca gacgctggat gccctggtcg ccgggaagct gggcgcccgc
      421 gacggccagg tcaccttcac ggccgaggag gtccggctgg gtgaccaggg tccgctcgac
      481 ccggcccaga ccgacctgct cgcctcccgg ttcaccttcc aggtgccgct gccggagctg
      541 ccggccgggg tgcgcctgga gcgggtcgag acccggccgg ggacggtggt gctgagcggc
      601 cgggcggcgt cggtcggggt ggccgcctga tacgctggct ggcatgaccc tggagttctg
      661 gtacacggcc ctgctggtcg cgatgctggc cgccgccggc ctgttcgccg tgtacgtggt
      721 ctaccggctg ctcagggact gagcgcggga ccaccggaga aaggaaagac ccagggagtc
      781 ggcattccgc tgcgcggcgc cacctcggtc ggaccaggcc gaggctccct gggttcggtg
      841 agctgtttgg attccggggg tgatccattc cccacgatcg aggctgagtc gccctaccgg
      901 gcagtcacct cacgggtccg taccggttct gacagctgga ccacctccct tctcgtgtac
      961 gcccagttag ccacacgtga atccggaccg caaacgacta tggctaccat ggggccatgg
     1021 cagcactgac cagcatcgtc gaccggccgg acctggcaac ggtggccctc gccgggaacg
     1081 acgtgctgcg ctacctccac gccgtctgca cccagcacac cctcgacctg gaaccgggcg
     1141 acgccaccca ggcgctgctg ctgtcgccca agggaaaggt cgagttcgcc ttccggctgg
     1201 ccgtgctgga cgagggcgtc ctgctcgaca ccgaggcgga ggcggccggg gcgctggccg
     1261 agcggctggc ccggttcgtg ttccgccacg acgtgaccgt cggccagccg gtcgtgggag
     1321 ccgcgtccct gctcggcccc ggcgccggcg acctcctggc cgcggccggc ctgcccgtcc
     1381 cggcccccgg ccgggccggc gtcggcggcc ccgaccttgt cgtccaccac accccggccg
     1441 ggttcgacct ggtcggcccg ggcgcactgg tcgtcttcgc cgccctggag cgggccgggg
     1501 cggagcggcg cccggccgag ctgtgggagc aggtccgggt cgagctcggc ctgccgaggg
     1561 cgggccggga gctgaccgac gacgtgctcg ccgaggaggc cgggctgctc ggctcccacg
     1621 tccacctgga caagggctgc tacccgggcc aggagacggt ggccagggtc cacaacctgg
     1681 gccaggtcca gcgccgcctg gccggcctcc ggtttcaccc gcacggagat gggggccagg
     1741 gccggagcct cgacaccgag cccgcgggcg ccggggacgg cgacggcctg cccacccccc
     1801 ggaccgacct ggtcgccgat gacggccgcc gcgccggcca gctccgcagc gtcgtcgacc
     1861 acccccgcct gggcccgatc ggcctggcct acgtccgccg ggtggtcgac agcggccgcc
     1921 tggtccgggc cggggaccgg gtcgcgacgg tcgtggacct gcccttccac tagcggtccg
     1981 tcgctaaagc gagcgacgtc gaccaggttg tgctagcgat caccctcgat cgagaagagg
     2041 ttcccgtcgg gatccttgaa ccaggcga
//
LOCUS       DASSWR010000073         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_285235,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000073 DASSWR010000000
VERSION     DASSWR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285235"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFO47_01225"
     CDS             complement(<1..340)
                     /locus_tag="VFO47_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET8787352.1"
                     /translation="MGRLRMAAAAATLLLAACTGGEPDPPAPAATAPAPTRAGDPAWR
                     TLAAAPSERTEVAAAAVGGRIWVLGGYAPDGATLATVEVYDTTADTWARGPDLPVAVN
                     HAMAATLDGVL"
     gene            393..1859
                     /locus_tag="VFO47_01230"
     CDS             393..1859
                     /locus_tag="VFO47_01230"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET8787353.1"
                     /translation="MSIAPSEQAVVDAVTPRLLVGGDWRQAGGGGTFPVEDPATGEVL
                     CEVADGTPEDGMAALDAAVAAQAEWAAHPPRERGEVLRRAFQLLNDRADDLALLMTLE
                     MGKPLAESKAEIAYAAEFFRWFAEEAVRIDGRYAVAPNGKGRLLTMRQPVGPCLLITP
                     WNFPMAMGTRKVGPAVAAGCTMVIKPAALTPLSMLALGGILEEAGLPPGVCNIITTKR
                     SGAVMEPLIRDGRVRKLSFTGSTEVGRSLLAQAADNVLRVSMELGGNAPFLIFDDADL
                     DAAVEGAMIAKMRNIGEACTAANRFHVAEPVAEAFAGKLAERMGAMKVGRGTEDGVTV
                     GPLIDKDAVDKVSELVSDALDKGARAVVGARPGDGRGYFYLPTVLADVPGDARVLKEE
                     IFGPVAPVASFDDEEAAVAAANDTEFGLVAYVYTRDLKRAFRVVEGLETGMVGLNQGL
                     VSNPAAPFGGVKQSGVGREGGPEGIAEYLETKYVAMNL"
     gene            1923..2696
                     /locus_tag="VFO47_01235"
     CDS             1923..2696
                     /locus_tag="VFO47_01235"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787354.1"
                     /translation="MRQTRWGGRLAALLLGAPLALAFPEPGWWWLALVGLAPLLALAL
                     AAPSGREAAVRAWLGGTGFFLAVNAFLVPKVGPFMVPLAMLLAVLWLPVGWLAWALLG
                     GLARPGPARLAAALVLVPSAFVAGEALRSWEGLGGPWGLLGASQWNAPALLSVAALGG
                     IWGLGFLLVAVNLAVAAAARPRVPAATRAVAAATAVALPLAALAFWALRPAPAPAGTA
                     AVACGPAPAGRGQPRRPAGPGPASRAAARARPGAWPAAP"
BASE COUNT          303 a         1000 c         1067 g          338 t
ORIGIN      
        1 acagcacccc gtccagggtg gcggccatgg catggttcac ggccaccggc aggtcgggcc
       61 cccgagccca ggtgtcggcg gtggtgtcgt agacctcgac ggtggccagg gtggccccgt
      121 cgggggcgta gccgcccagc acccagatgc gcccgccaac ggcggccgcg gccacctcgg
      181 tccgctccga cggcgccgcc gccagcgtcc gccacgccgg gtcgccggcc cgggtgggcg
      241 ccggggcggt ggccgccggc gccggggggt ccggctcgcc gcccgtacag gcggccagca
      301 gcagcgtcgc cgccgccgcg gccatccgga gcctgcccat ggcgcgctac gctaacggcg
      361 tccgcgacgg gacgccagag gaggcagcgc ccatgagcat cgcaccatcg gagcaggcgg
      421 tcgtcgacgc cgtcaccccg cggctgttgg tcggcgggga ctggcgccag gccggcggcg
      481 gggggacctt cccggtcgag gacccggcca ccggcgaggt gctctgcgag gtcgccgacg
      541 gcaccccgga ggacggcatg gccgccctgg acgcggccgt ggccgcccag gcggagtggg
      601 ccgcccatcc gccgcgcgag cggggcgagg tcctgcgccg ggcgttccag ctcctcaacg
      661 accgggccga cgacctggcc ctgctgatga ccctggagat gggcaagccg ctggccgaga
      721 gcaaggccga gatcgcctac gcggccgagt tcttccgctg gttcgccgag gaggcggtcc
      781 gcatcgacgg ccgctacgcc gtcgccccca acggcaaggg gcggctgctg accatgcgcc
      841 agcccgtggg gccgtgcctg ctcatcaccc cctggaactt ccccatggcc atggggaccc
      901 gcaaggtcgg cccggcggtg gccgccggct gcaccatggt catcaagccg gccgccctca
      961 cccccctgtc gatgctggcc ctcggcggga tcctggagga ggcgggcctg cccccggggg
     1021 tctgcaacat catcaccacc aagcggtccg gggcggtgat ggagccgctg atcagggacg
     1081 gccgggtccg caagctgtcg ttcaccggct ccaccgaggt cggccgcagc ctgttggccc
     1141 aggcggccga caacgtgctc cgggtgtcga tggagctggg cggcaacgcg cccttcctga
     1201 tcttcgacga cgccgacctg gacgcggccg tcgagggcgc gatgatcgcc aagatgcgca
     1261 acatcggcga ggcctgcacg gccgccaacc gcttccacgt ggccgagccg gtggcggagg
     1321 cgttcgccgg caagctggcc gagcggatgg gggccatgaa ggtcggccgg ggcaccgagg
     1381 acggcgtcac cgtcggcccc ctgatcgaca aggacgccgt cgacaaggtg tcggagctgg
     1441 tctccgacgc cctcgacaag ggggccaggg cggtcgtggg ggcccggccg ggcgacggcc
     1501 gcggctactt ctacctgccg acggtcctgg ccgacgtccc cggcgacgcc cgggtgctca
     1561 aggaggagat cttcgggccg gtggccccgg tggcctcctt cgacgacgag gaggcggcgg
     1621 tcgcggccgc caacgacacc gagttcgggc tggtcgccta cgtctacacc cgcgacctga
     1681 agcgggcctt ccgggtcgtg gagggcctgg agacgggcat ggtcggcctc aaccaggggc
     1741 tggtgtcgaa cccggccgcc cccttcggtg gggtcaagca gtccggcgtc ggccgcgagg
     1801 gcggccccga gggcatcgcc gagtacctgg agaccaagta cgtcgccatg aacctctgac
     1861 cggccggccg atggggccgt gacgagcgcg gtgggcgcat acgagacctg ctggagatga
     1921 gggtgcgcca gacacgctgg ggcggccggc tggcggcgtt gctgctgggg gcgccgctgg
     1981 ccctggcctt cccggagccc gggtggtggt ggctggccct ggtcgggctg gcgccgctgc
     2041 tggccctggc cctggccgcc cccagcgggc gcgaggcggc cgtccgggcc tggctcggcg
     2101 ggaccgggtt cttcctggcc gtcaacgcct tcctggtgcc gaaggtgggg ccgttcatgg
     2161 tcccgctggc catgctcctg gccgtgctct ggctgccggt cggctggctg gcctgggcgc
     2221 tcctcggggg cctggcccgc cccggcccgg cccggctggc cgccgccctg gtgttggtgc
     2281 cgagcgcgtt cgtggccggg gaggcgctgc ggtcctggga ggggctgggc ggcccctggg
     2341 gcctgctcgg ggccagccag tggaacgccc cggcgctgct gtcggtggcc gcgctcggcg
     2401 ggatctgggg gctcggcttc ctgctggtgg ccgtcaacct ggccgtcgcc gccgccgccc
     2461 gcccgcgggt cccggccgcg acccgggccg tggccgcggc caccgccgtc gccctgcccc
     2521 tggccgcgct cgccttctgg gcgctgcgac cggccccggc gccggccggg acggccgcgg
     2581 tggcgtgtgg gccggcgccg gcgggtcggg gtcagccgcg ccgcccagca gggcccgggc
     2641 cagccagccg agcggcagcc agagcacggc caggagcatg gccagcggca ccatgaacgg
     2701 cccgacgg
//
LOCUS       DASSWR010000074         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1075763, whole genome shotgun sequence.
ACCESSION   DASSWR010000074 DASSWR010000000
VERSION     DASSWR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075763"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1297
                     /locus_tag="VFO47_01240"
     CDS             122..1297
                     /locus_tag="VFO47_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787355.1"
                     /translation="MTVGTPRPPLVSRALLLVFVASFGAMTGFYLLLSVVPLYADSVG
                     AGGVGAGLTTGALMAATVAGELAVPRLAARFGYRAVFAAGLVLLGAPALALPASTTLA
                     AILAVSVVRGLGFAIVVVVGGALVASLVPGERRGEGLGLFGIVVGVPGVAALPLGVWL
                     VGEAGYPAVFAAGAAAALAGLIVVPGLPGRAARREAPVGVLAGMRTPALVRPAAAFAV
                     AAMAAGVVVTFLPLATGGSSGRLAAVALFVQAAASTGSRWWAGRYGDRHGPAVLLVPG
                     VVATAAGIATLVMVDRPAAVLAGMAVFGCGFGVTQIATMTVMLSRVTPSGFGTVSAIW
                     NLAYDLGIGAGAFGFGVVAAQTGYPAAFAVTAALVLAGLVPLWLDRRRPATGSCIHS"
     gene            1349..1969
                     /locus_tag="VFO47_01245"
     CDS             1349..1969
                     /locus_tag="VFO47_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8787356.1"
                     /translation="MSSAAEKAYEAIRDGIVGGELPVGGRLREEELAGSIGVSRTPVR
                     EALRRLDAEGLVEFSPHRGAQVASWSDQDLDEIFGLRAVLESYGASLAATRVTPEALA
                     GLAGLADAMEAASGRGQLDQVAELNNRFHRGVLAASGNQRLAGLLSGVVVVSLVRRTF
                     HRYSPEALARSHAHHRELLAALAAGDPEWAASVMRSHVLAARAVLR"
     gene            1966..>3175
                     /locus_tag="VFO47_01250"
     CDS             1966..>3175
                     /locus_tag="VFO47_01250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8787357.1"
                     /translation="MSTAGAPEAAGGHRGAPGRGPLDDVRVVEMGQLLAGPFCGQLLA
                     DFGAEVIKLEDPAKGDPMRQWGREKPHGLSLWWPVIARNKKSVTLNLRHPEGQALARR
                     LLEEADVLVENFRPGTLERWGLGYDALAERNPGLVLVRVTGFGQDGPYAPRAGYGSIG
                     EAMGGLRYVTGDPSTPPSRSGISIGDSLAGTFAALGTMLALHARGRSGRGQVVDSAIY
                     EAVLAMMESLVTEYQVAGYTRERTGAILPNVAPSNVYPTADGQLVLVAANQDTVFRRL
                     AAVMGRPELADDPRYASHSARGEHQEELDGLIAGWTATLGADRLLHVLEEVGVPAGRI
                     YRAADMLADPHYLARQAIVRLVDPELGEIAMQNVAPRLSATPGRVVWPGPALGRHNQE
                     VYQGLLGLPDE"
BASE COUNT          318 a         1155 c         1267 g          435 t
ORIGIN      
        1 gctaccggca gctggtcggc atcgtccagg accgccccgg cgaggagctc gccgcccggg
       61 tggccgacta cgcctgggtc atggccgccc tccgccaccg cgaccgcgcc ggcgcgtcca
      121 cgtgacggtc ggcaccccgc ggccgccgct cgtcagccgc gccctgctgc tggtgttcgt
      181 ggcctcgttc ggggccatga ccggcttcta cctgctgctg tcggtggtcc cgctgtacgc
      241 cgactcggtc ggggccggtg gggtcggggc cgggctgacc acgggggcgc tgatggcggc
      301 cacggtggcc ggcgagctgg ccgtcccccg cctcgcggcc aggttcgggt accgggcggt
      361 gttcgcggcc ggcctggtcc tgctgggcgc gccggccctg gccctgcccg cctcgaccac
      421 cctggccgcg atcctggccg tcagcgtcgt gcggggactg gggttcgcga tcgtggtggt
      481 ggtcggcggg gcgctggtgg cgtcgctggt cccgggcgag cgccgcgggg aggggctggg
      541 gctgttcggg atcgtcgtcg gggtgcccgg ggtggcggcc ctgccgctcg gggtctggct
      601 ggtgggggag gccggctacc cggcggtgtt cgccgccggg gcggcggccg ccctggccgg
      661 gctgatcgtc gtccccggac tgcccggccg ggcggcgcgg cgggaggcgc ccgtcggcgt
      721 cctggccggg atgcgcacgc cggcccttgt ccggccggcg gccgccttcg cggtggcggc
      781 catggcggcc ggggtggtgg tgaccttcct gcccctggcc accggcggga gctcgggccg
      841 gctggccgcg gtggcgctgt tcgtccaggc ggccgcgtcc accggcagcc gctggtgggc
      901 cgggcggtac ggcgaccggc acgggccggc ggtcctgctc gtccccgggg tcgtggccac
      961 cgccgccggc atcgcgaccc tggtcatggt cgaccgcccg gcggccgtcc tggccgggat
     1021 ggccgtgttc gggtgcgggt tcggggtcac ccagatcgcc accatgacgg tgatgctgag
     1081 ccgggtgacg ccgtccgggt tcgggacggt gagcgccatc tggaacctcg cctacgacct
     1141 gggcatcggg gccggtgcct tcggcttcgg ggtggtcgcg gcccagaccg gctacccggc
     1201 ggcgttcgcc gtcacggccg cgctggtgct ggccgggctc gtccccttgt ggctggaccg
     1261 gcggcggccc gcgacgggca gttgtataca ttcctgacgt agccaggtcg cagttggcct
     1321 tgatccgctt gtgttgtata caacccctat gtcgagcgcg gccgagaagg cgtacgaggc
     1381 catccgcgac gggatcgtcg gcggggagct cccggtcggg gggcggctgc gcgaggagga
     1441 gctggccggc agcatcgggg tgagccggac gccggtgcgg gaggcgctgc ggcggctcga
     1501 cgccgagggg ctggtcgagt tctcgcccca ccggggcgcc caggtggcgt cctggtccga
     1561 ccaggacctg gacgagatct tcgggctgcg ggcggtgctg gagagctacg gggcgtcgct
     1621 ggccgcgacc cgggtcaccc ccgaggccct ggccgggctg gccgggctgg ccgacgccat
     1681 ggaggcggcc agcggccgcg gccagctcga ccaggtggcc gagctcaaca accgcttcca
     1741 ccggggcgtg ctggccgcct cgggcaacca gcggctcgcc gggctgctgt cgggcgtggt
     1801 ggtggtctcg ctggtccggc ggaccttcca ccgctactcg cccgaggccc tggcccgcag
     1861 ccacgcccac caccgggagc tgctggccgc cctggccgcc ggcgaccccg agtgggccgc
     1921 ctcggtgatg cgctcccacg tcctggccgc ccgggcggtg ctgcgatgag cacggcgggc
     1981 gcaccggaag ccgctggcgg gcaccggggt gcgccaggcc gcggcccgct ggacgacgtg
     2041 cgggtggtcg agatgggcca gctcctggcc gggccgttct gcggccagct gctggccgac
     2101 ttcggggccg aggtgatcaa gctcgaggac ccggccaagg gcgacccgat gcgccagtgg
     2161 gggcgggaga agccgcacgg cctgtcgctg tggtggccgg tgatcgcccg caacaagaag
     2221 tcggtcaccc tgaacctgcg ccacccggaa ggccaggccc tggcccggcg gctgctggag
     2281 gaggccgacg tcctggtcga gaacttccgc cccggcaccc tggagcgctg ggggctcggc
     2341 tacgacgccc tggccgagcg gaacccgggg ctggtgctgg tccgggtgac cgggttcggc
     2401 caggacggcc cgtacgcccc ccgggccggc tacggctcca tcggtgaggc catgggcggg
     2461 ctgcgctatg tcaccggcga cccctcgacc ccgccgagcc gctcgggcat ctccatcggg
     2521 gacagcctgg ccgggacctt cgccgccctc gggaccatgc tggccctgca cgcccgcggg
     2581 cgcagcgggc gcggccaggt ggtcgactcg gccatctacg aggccgtcct ggccatgatg
     2641 gagtcgctgg tcaccgagta ccaggtggcc ggctacaccc gcgagcggac cggcgccatc
     2701 ctgcccaacg tcgccccctc caacgtctac ccgaccgccg acgggcagct ggtgctggtc
     2761 gccgccaacc aggacacggt gttccggcgg ctggccgccg tgatgggccg gcccgagctg
     2821 gccgacgacc cccgctacgc cagccactcg gcccgcggcg agcaccagga ggagctggac
     2881 gggctgatcg ccggctggac ggccaccctg ggcgccgacc ggctcctgca cgtcctggag
     2941 gaggtgggcg tgccggcggg gcggatctac cgggccgccg acatgctcgc cgacccccac
     3001 tacctggccc gccaggcgat cgtccgcctg gtcgacccgg agctggggga gatcgccatg
     3061 cagaacgtgg ccccgaggct gtcggccacc ccgggccggg tcgtctggcc ggggccggcc
     3121 ctcggccggc acaaccagga ggtgtaccag gggctgctcg gcctgcccga cgagg
//
LOCUS       DASSWR010000075         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1048792, whole genome shotgun sequence.
ACCESSION   DASSWR010000075 DASSWR010000000
VERSION     DASSWR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048792"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="VFO47_01255"
     CDS             <1..1942
                     /locus_tag="VFO47_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET8787358.1"
                     /translation="GWWVVGIGGVAGVALGTGLPIGMIVAFAVGIGSAAIVHLLFGSP
                     AGRLTLDQVGGALAELGVDATRLRQAPLEPRGVAVAFAEATDGRSLLVKIYGRDAWDG
                     QLLASVWSSLWYRDDGPHLALGRRQQVEHEAFVTLLAERAGVAVLPVVAAGMASQRDA
                     LLVTEITGRPLRTLDPGEVDDELLGGIWANAGRLHALGVAHRRLDGGRIVVRPDGTPA
                     FGDFGGAEADADDADVAADRSQILVATALVAGPERAAAAALAALGSDALARVLPLVQP
                     AALERATRRAVKEQDWDLDDLRKACADASGVELPKLAQLRRVSLRSIGIVVLIALVAY
                     AMISAVANVGLDNLVDEFKGADLGWLAAATLLSPVIPVAQTVATLGASFRPLRFGPVL
                     MLEYAIQFIALAVPSSAARLALDVRFFGRNGIEGGAALSIGVIASVCGFVIQVLLVLV
                     TSLSGLASLDLGGGDVTATSSTSDSSESGGHRLLILAAVLVVLGLLATLAVPRYRRAV
                     REAVPRYREMLRAQASSAATALRVLRSPSKVVMIFAGNLAAQLLQAVILGLCLRAFGH
                     HATLAELILVNTLASLFAGFMPVPGGMGVAEAAYTAGLVALGVPNAPAMSTAIAFRMV
                     TYYLPPVWGAVAMRWLRQHAYL"
BASE COUNT          237 a          802 c          791 g          339 t
ORIGIN      
        1 gggatggtgg gtggtcggga tcgggggggt cgccggggtc gcgctgggaa ccggcctccc
       61 catcgggatg atcgtggcct tcgccgtcgg gatcgggtcg gccgccatcg tccacctgct
      121 gttcggctcc ccggcgggcc ggctcaccct cgaccaggtc ggcggtgccc tggccgagct
      181 gggcgtcgac gcgaccaggc tccgccaggc gccgctggag ccccgtggcg tggccgtcgc
      241 ctttgccgag gcgacggacg ggcgctccct gctggtcaag atctacggcc gggacgcctg
      301 ggacggccag ctgctcgcct ccgtctggtc ctcgctgtgg taccgggacg acgggccgca
      361 cctcgcgctg ggccgccgcc agcaggtcga gcacgaggcg ttcgtcaccc tgctggccga
      421 gcgggccggc gtcgcggtgc tcccggtggt ggcggccggg atggcgtccc agcgcgacgc
      481 cctgctcgtg accgagatca ccggccgccc gctgcgcacg ctcgaccccg gcgaggtaga
      541 cgacgagctc ctggggggca tctgggccaa cgccgggcgg ctccacgccc tgggggtcgc
      601 gcatcggcgg ctcgacggcg gccgcatcgt ggtccggccc gatgggacac cggccttcgg
      661 cgacttcggg ggcgcggagg cggacgccga cgacgccgac gtcgcggccg accggtcaca
      721 gatcctggtc gcgaccgcgc tggtggcggg gccggaacgc gccgccgcgg ccgcactggc
      781 cgcgctgggc agcgacgccc tcgcgcgggt gctccccctg gtgcagccgg cggcgctgga
      841 gcgggcgacc cggcgcgcgg tcaaggagca ggactgggac ctcgacgacc tgcggaaggc
      901 ctgcgccgac gcctcgggcg tcgagcttcc gaagctggcc cagctgcggc gggtgtcgct
      961 ccggtcgatc ggcatcgtgg tcctgatcgc gctggtcgcc tacgcgatga tctccgcggt
     1021 ggccaacgtc gggctggaca acctggtcga cgagttcaag ggggcggacc tcggctggct
     1081 ggccgccgcg acactgctct caccggtcat cccggtggcc cagaccgtgg ccaccctcgg
     1141 cgcctcgttc cggccgctcc ggttcgggcc cgtcctcatg ctcgagtacg ccatccagtt
     1201 catcgccctg gccgtgccga gctcggcggc ccggctcgcc ctcgacgtgc gcttcttcgg
     1261 gcgcaacggc atcgagggcg gcgccgcgct ctccatcggc gtgatcgcca gcgtctgcgg
     1321 gttcgtcatc caggtcctgc tggtcctggt gaccagcctg tccgggctgg cctccctgga
     1381 cctggggggc ggcgacgtca cggccacgtc ctcgacgagt gactcgtcgg agtccggcgg
     1441 ccaccggctg ctgatcctgg ccgcggtcct ggtcgtgctc ggcctgctcg ccacgctggc
     1501 cgtgcccagg taccggcggg cggtccggga ggccgtcccc aggtaccgcg agatgctccg
     1561 tgcccaggcc tcgtcggccg ccacggcgct gcgggtcctg cgctcgccgt ccaaggtcgt
     1621 gatgatcttc gccggcaacc tggcggcgca gctgctccag gccgtcatcc tcggcctctg
     1681 cctgcgggcg ttcgggcacc acgccaccct ggccgaactg atcctggtga acaccctggc
     1741 gagcctgttc gccgggttca tgcccgtgcc cggcggcatg ggcgtcgccg aggccgccta
     1801 cacggccggg ctggtcgccc tgggtgtgcc caacgccccc gcgatgtcca ccgcgatcgc
     1861 cttccggatg gtcacctact acctcccccc ggtctggggc gcggtcgcca tgcgctggct
     1921 gcgccagcac gcctacctct gaacggacct ctcgccggtt ccttcagagg cgctgttgca
     1981 tctggacggt tcacggcata ttcaagccgc ggccaccagt tcgggatcag atcgccaaag
     2041 tgaggtggtc ggacgtgaca gtcagccgcc gccgaccggc acctcgaccg tcagttcgta
     2101 gtgacctcgg cggaggttct gcacggacgc atggccggca attgatgacc ctggcgctac
     2161 gggtcctgc
//
LOCUS       DASSWR010000076         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_314186,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000076 DASSWR010000000
VERSION     DASSWR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314186"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1400)
                     /locus_tag="VFO47_01260"
     CDS             complement(75..1400)
                     /locus_tag="VFO47_01260"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8787359.1"
                     /translation="MDIDIRPITPEQGDEFSAVMAAAFGESFSEEELADHDRWFEYDR
                     SIGAFDGDRLVGTGGAYSMDMTLPGLTTIPIGGLTAISVLPTHRRRGILRAMIAYHFQ
                     EVEGRGELVSGLGASESLLYGRYGYGLATTSADYEIDPRRGQFLRPFAGRGRLRLLGP
                     EETAKVVPALYDRYRRGQPGELSRPQAWWDTYARDPEWTRRGASRHYDVVHESEPGRV
                     DGWLGYRVESRWPDGMAANIIKVGSLIGLTPEAEGALWRYLLDLDLAGTVKLFDRPLD
                     DPARWRLADPRRLRVTQVGDQLWLRLLDLPGALGARRYAVSGSLVLEVTDALRPRNQG
                     RFRLEGGPDGATCEPTRSAPDLGLDVADLGAAYLGGTSLVSLARAERVTELTPGALLR
                     ADRMFAASRLHHALLAGVVPSGVVPVLRVDSVTPVEAPADPYEHNLYGL"
     gene            1558..1704
                     /locus_tag="VFO47_01265"
     CDS             1558..1704
                     /locus_tag="VFO47_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018219920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787360.1"
                     /translation="MDLAYLDAASGSLIVQAIVAGAAGAAVFFKLGWRRLTGPFRNRQ
                     SDKA"
     gene            1714..>3103
                     /locus_tag="VFO47_01270"
     CDS             1714..>3103
                     /locus_tag="VFO47_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8787361.1"
                     /translation="MEQEGRPVARRERLWAFFELFALVGFVVAQPTLDVLGKAPDFFI
                     FRRAGRAEILILVLAVTLLPALGLWLAEQVVGLAGERARRIVHVLMVGGLLAVLALEV
                     GKNLTSVRGPGLVAIGVAGGVAGGLLYANWDGFKLWLRYLAPAPLAFALLFLLVSPVS
                     KLVLPQPEGAVDANSPGVRINPEHPVVMILLDEFPVTSLLDSRGQIDARLYPNFAKLA
                     GQSTWYRNATAVSGLTNWAVPSMLTGRYPTKAQEEASPTAAQYPDNLFTLLGKSYDVE
                     QFQVVTQLCPAEVCKATRDSAPSGAGLKTTLRDSARVFKRIVTPRDVDENPTGAWLTA
                     DPEAEREPRAARGGGPGSPILREIEQGNESRQYYNFVKSIEGSDPPTFYFMHLLLPHQ
                     PWRYLPDGRIYSDRGDGRNANGWTSEPWPPRLSRQRHLMQVAETDRLLGQVMRRLQEQ
                     GMYDDTLFVVTAD"
BASE COUNT          414 a         1124 c         1128 g          437 t
ORIGIN      
        1 cctggtcccg tccccgttac ctcccatgcc ggcagtctag caatgcagtt gcaactcgat
       61 gcagctccgg cgtctcacag accgtagagg ttgtgctcgt aggggtcggc cggggcctcg
      121 accggcgtga ccgagtcgac ccgcagcacc ggcaccacgc ccgatggtac gaccccggct
      181 agaagtgcgt ggtgcagacg ggaggcggcg aacattcggt cggcccggag gagggcgccg
      241 ggggtgagct cggtgacccg ttcggcccgg gccagggaga cgaggctggt cccgccgagg
      301 taggcggcgc cgaggtcggc gacgtccagg ccgaggtcgg gggcggaccg ggtgggctcg
      361 caggtggcgc cgtcggggcc gccctcgagg cggaagcggc cctggttgcg ggggcgcagg
      421 gcgtcggtga cctcgagcac cagcgacccg gagaccgcgt agcggcgggc gccgagcgcg
      481 cccggcaggt cgagcagccg cagccagagc tgatcgccca cctgggtgac ccgcagccgc
      541 cgcgggtcgg ccagccgcca gcgggccggg tcgtcgagcg gccggtcgaa cagcttcacg
      601 gtgccggcca ggtccaggtc gaggaggtag cgccacaggg cgccctcggc ctccggggtg
      661 agcccgatca ggctgcccac cttgatgatg ttggcggcca tgccgtcggg ccagcggctc
      721 tcgacccggt agccgagcca gccgtcgacc cggcccggtt ccgactcgtg gaccacgtcg
      781 tagtggcggc tggccccgcg ccgggtccac tccgggtcgc gggcgtaggt gtcccaccac
      841 gcctggggac gggacagctc gcccggctgg ccccgccggt agcggtcgta gagggccggc
      901 acgaccttgg ccgtctcctc cggcccgagc aggcgcagcc gcccccggcc ggcgaacggg
      961 cgcaggaact gcccgcgccg agggtcgatc tcgtagtcgg cggaggtggt ggccaggccg
     1021 tagccgtagc gcccgtagag cagcgactcg gacgccccca ggcccgagac cagctcgccc
     1081 cggccctcga cctcctggaa gtggtaggcg atcatcgccc gcaggatgcc ccgccggcgg
     1141 tgggtcggga gcaccgagat ggcggtcagg ccgccgatcg ggatggtggt gagcccgggc
     1201 agggtcatgt ccatcgagta ggcgccgccg gtgccgacga gccggtcgcc gtcgaaggcg
     1261 ccgatgctgc ggtcgtactc gaaccagcgg tcgtggtcgg ccagctcctc ctcggagaag
     1321 gactccccga aggcggcggc catgacggcg ctgaactcgt cgccctgctc gggcgtgatg
     1381 ggccggatgt cgatgtccat cgctccgttg tactggaaac gcgggcggcg cacacctggt
     1441 tttctcgtga tatcgtcctc tgcttgaccg tgacatcccg tcagtgggtc cacggaatcc
     1501 cccgaccgtg ccgctcccgc ctgggatgcg cccgcttgcg caaggagttc gaacgcgatg
     1561 gacctggcct acctcgatgc cgcctcgggc agcctgatcg tgcaggcgat cgtcgccggc
     1621 gccgccggag cggcggtgtt cttcaagctc ggctggcgcc ggctgaccgg cccgttccgc
     1681 aaccgccaga gcgacaaggc ctagccgcac cccatggagc aggaagggcg gcccgtcgcc
     1741 cgccgcgagc ggctctgggc cttcttcgag ctgttcgcgc tggtcgggtt cgtggtcgcc
     1801 cagccgaccc tggacgtgct cggcaaggcc cccgacttct tcatcttccg gcgggccggc
     1861 cgggccgaga tcctgatcct ggtcctggcc gtgaccctgc tcccggccct cggcctgtgg
     1921 ctggccgagc aggtcgtcgg gctggccggg gagcgggccc gccgcatcgt gcacgtgctg
     1981 atggtcggcg ggctgctggc cgtgctcgcc ctggaggtcg gcaagaacct gacctcggtc
     2041 cgcgggcccg ggctggtcgc catcggcgtg gccggcgggg tggccggcgg gctgctgtac
     2101 gccaactggg acggcttcaa gctgtggctg cgctacctgg ccccggcgcc gctggccttc
     2161 gccctgttgt tcctgctcgt ctcgccggtg tccaagctgg tgctgcccca gcccgagggc
     2221 gccgtggacg ccaactcgcc cggggtgagg atcaacccgg agcacccggt ggtgatgatc
     2281 ctcctggacg agttcccggt gacctcgctg ctggactcca gggggcagat cgacgcccgc
     2341 ctctacccga acttcgccaa gctggccggc cagtccacct ggtaccgcaa cgccaccgcg
     2401 gtgtcgggcc tgaccaactg ggccgtgccg tcgatgctga cggggcgcta cccgaccaag
     2461 gcccaggagg aggcctcgcc gacggccgcc cagtaccccg acaacctgtt caccctgctc
     2521 ggcaagtcct atgacgtcga gcagttccag gtcgtcaccc agctctgccc ggccgaggtg
     2581 tgcaaggcga ccagggactc ggccccgtcc ggggccggcc tgaagacgac cctgcgcgac
     2641 tcggcccggg tcttcaagcg gatcgtgacg ccgcgggacg tcgacgagaa cccgaccggg
     2701 gcctggctga ccgccgaccc cgaggccgag cgcgagcccc gggcggcccg gggcggcggg
     2761 ccggggtcgc ccatcctgcg cgagatcgag cagggcaacg agtcgcgcca gtactacaac
     2821 ttcgtgaagt cgatcgaggg ctccgacccg cccaccttct acttcatgca cctgctgctg
     2881 ccccaccagc cctggcgcta cctgcccgac gggcgcatct acagcgaccg cggcgacggc
     2941 cgcaacgcca acggctggac cagcgagccc tggccgccgc ggctgtcgcg ccagcgccac
     3001 ctcatgcagg tcgccgagac cgaccggctg ctcggccagg tcatgcgccg gctccaggag
     3061 caggggatgt acgacgacac cctgttcgtg gtcaccgccg acc
//
LOCUS       DASSWR010000077         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_398760,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000077 DASSWR010000000
VERSION     DASSWR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398760"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFO47_01275"
     CDS             <1..421
                     /locus_tag="VFO47_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET8787362.1"
                     /translation="NAALAITVGATHRDMPHTYGVSYFSSKGPTGDGRLKPDLVAPGE
                     RITSCAAGKALTSAFPGGQAPPPGRTAVYVEDSGTSMAAPHVSGAVANLLSIRREFIG
                     RPDEVKRIFCESATSLGRERYFEGHGLVDLMRAIQSI"
     gene            434..1771
                     /locus_tag="VFO47_01280"
     CDS             434..1771
                     /locus_tag="VFO47_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-threonine protein kinase"
                     /protein_id="HET8787363.1"
                     /translation="MASEIAGQPHWEVGFDEKGKPDQAGVDALLAELPGKDLTDLFVF
                     AHGWNSDFRQARRLYQLYFQQVPALLARGGGQAARVGTLGVVWPSKRWADEPEPTGAV
                     DGGGAAGFGGAAGFGDGPVAEAPPADPVLVEDLKDVFEGAERHQALDELARLLEERPE
                     DEAALARFQALMKELAGEPDAEPADEDQGELALLTDDPEEVFGRFADAVPRGGGPAAG
                     FGDTFGRLWDGAKEALRQLTYFEMKKRAGVVGKEGLGPLLGRIHQADPDVRIHLIGHS
                     FGARLVSFALAGLPDGDSPVKSLYLLQGAFSHFAFADALPMDRSRGGALKGMAARVDG
                     PLVASFTVHDTAVGKLYPMASLSSRDDAAGLEDRLYRWGGIGHDGAQAVDATVAGLGP
                     VGTGYPFQRGRFVNLDGNAIVNRGGPPAGAHSDIFHPELVWAGLAAAGLVTAT"
BASE COUNT          255 a          793 c          737 g          262 t
ORIGIN      
        1 caacgccgcc ctggccatca ccgtcggcgc cacccaccgg gacatgcccc acacctacgg
       61 ggtgtcgtac ttctcctcca agggcccgac cggcgacggc cggctcaagc cggacctggt
      121 cgcccccggc gagcgcatca cctcctgcgc cgccggcaag gcgctgacca gcgcctttcc
      181 cggcggccag gcgccaccgc ccggccgcac ggccgtgtac gtcgaggact ccggcaccag
      241 catggccgcc ccccacgtct cgggggcggt ggccaacctc ctgtccatcc gccgcgagtt
      301 catcggccgc cccgacgagg tcaagcggat cttctgcgag tcggccacct ccctgggccg
      361 cgagcgctac ttcgagggcc acggcctggt cgacctgatg cgcgccatcc aatcgatctg
      421 agaggagcca accatggcga gcgagatcgc cggccagcct cactgggagg tcggcttcga
      481 cgagaagggc aagcccgacc aggccggggt cgacgccctg ctggccgagc tgccgggcaa
      541 ggacctgacc gacctgttcg tgttcgccca cggctggaac agcgacttcc gtcaggcccg
      601 ccggctctac cagctctact tccagcaggt cccggccctt ctcgcccggg gcgggggcca
      661 ggccgcccgg gtcgggaccc ttggggtggt gtggccgtcc aagcgctggg cggacgagcc
      721 cgagccgacc ggcgccgtgg acggcggcgg cgccgccggg ttcgggggtg ccgccggatt
      781 cggcgacggc ccggtggccg aggctccgcc cgccgacccg gtcctggtcg aggacctcaa
      841 ggacgtgttc gagggcgccg agcggcacca ggccctcgac gagctggccc ggctgctgga
      901 ggagcggccc gaggacgagg ccgccctggc ccggttccag gccctgatga aggagctggc
      961 cggggagccg gacgccgagc cagccgacga ggaccagggc gagctggccc tgctcaccga
     1021 cgaccccgag gaggtgttcg ggcgcttcgc cgacgccgtg ccccgcggcg gcggcccggc
     1081 ggccggcttc ggcgacacct tcgggcggct ctgggacggc gccaaggagg cgctgcgcca
     1141 gctcacctac ttcgagatga agaagcgggc cggggtggtc ggcaaggagg gccttggccc
     1201 gctgctcggc cgcatccacc aggccgaccc cgacgtgcgc atccacctga tcgggcacag
     1261 cttcggagcc cgcctggtct ccttcgccct ggccggcctg cccgacggcg actccccggt
     1321 caagtcgctg tacctgctgc agggcgcgtt ctcgcacttc gccttcgccg acgccctgcc
     1381 catggaccgc tcccgcggcg gcgccctcaa gggcatggcc gcccgggtcg acggcccgct
     1441 ggtggcctcg ttcacggtcc acgacaccgc cgtcggcaag ctctacccga tggcctcgct
     1501 gagctcccgg gacgacgccg ccggcctgga ggaccggctg taccgctggg gcgggatcgg
     1561 ccacgacggc gcccaggcgg tcgacgccac cgtggccggc ctcggcccgg tcgggaccgg
     1621 ctaccccttc cagcggggcc gcttcgtcaa cctcgacggc aacgccatcg tcaaccgcgg
     1681 cggcccgccg gccggcgccc actccgacat cttccatccc gagctggtct gggccgggct
     1741 ggccgcggcc ggtctggtca cggccaccta gacccggcgt cccctaccag cggttctgcc
     1801 tccgcgagta ccgcggggtg gtggagccgg cccacgcccc tgtccgggaa gcggccggat
     1861 caaggtcgac ccggccgcat cctgcggatc cggctcggct agctcaccac tcgaactcgc
     1921 ccgaggaccg gccggtgctg gggttgccga ggccggtgcc gggctgggcg ccggggttcg
     1981 gctggcgcgg cggggagatc gtcgggccgg tggcgccgct gtcgcggggc gaggcctcca
     2041 gcagggc
//
LOCUS       DASSWR010000078         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_992424,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000078 DASSWR010000000
VERSION     DASSWR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992424"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFO47_01285"
     CDS             <1..694
                     /locus_tag="VFO47_01285"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET8787364.1"
                     /translation="VDTQLCANAPYRFLVSGMGDALATWVEARATAEARKSTMAGGLP
                     TMAGLALARLSWDTLIAYGFLARQAAQQHVVTPALEKVVEANTLLSGLGFESGGLAAA
                     HAVHNGLTALPQLHHFMHGEKVNFGTLTQFALEDRPTREFNEFVSFCMSVGLPTTFAD
                     LDLGTVSREELETVAKAATASAETIHNMPFAVTPGMVVDAMVAADAYSRAYQQESGQQ
                     PAAWSRPSADGH"
     gene            738..1805
                     /locus_tag="VFO47_01290"
     CDS             738..1805
                     /locus_tag="VFO47_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET8787365.1"
                     /translation="MGAEAIGVGLAGYGLGGSVFRAPLVQAAPGLRLHAVATSRAGQV
                     GRDLPGVRVVASAEELLEDPAVELVVVAAPNAVHHRLAAAALRAGRHVVVDKPFTLST
                     AEADELIALAEAADRRLSVFHNRRWDGDFLTVRRCLEAGLLGEVSSFVSRYDRFRPVP
                     KGGWKEEAVPGSGVLWDLGPHLIDQALQLFGMPATVWADLAVQRPGVEAVDYVHLVLG
                     YGRLRVLLHAAMEVRHPGPRFEVHGDRGSFVTWGMDTQEAALRAGARPGDPGWGTEPP
                     DRHGTLTTEVAGLGLHARVTAVPGDYGAFYAAMAAAVAGEGPVPVAPSEARATVAVTE
                     HALRSSREGRVVEVAAAAPAS"
     gene            complement(1802..>2332)
                     /locus_tag="VFO47_01295"
     CDS             complement(1802..>2332)
                     /locus_tag="VFO47_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007821151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET8787366.1"
                     /translation="GAGVLTPASWGMFSLRLPPFTPPRQPDEDHKVLLEALVELAGHA
                     AAEGVWLAVEPINRYEDYMVNRLDQAVELGEEVERATGQDSVRVCADLFHMNIEEDDL
                     AAAIRAAGPRIAHVHVDDTNRLQPGTGHMDFGSVFAALRDVGYDDWLTFECRLRGAPE
                     EVLPASTRFLAGFLSG"
BASE COUNT          282 a          874 c          869 g          307 t
ORIGIN      
        1 ggtcgacacc cagctgtgcg ccaacgcgcc ctaccggttc ctggtctcgg ggatgggcga
       61 cgccctggcc acctgggtcg aggcccgggc caccgccgag gcccgcaagt cgaccatggc
      121 cggcgggctg ccgaccatgg ccgggctggc cctggcccgg ctgagctggg acacgctgat
      181 cgcctacggc ttcttggccc ggcaggccgc ccagcagcac gtggtcacac cggccctgga
      241 gaaggtggtg gaggccaaca ccctgctgtc ggggctgggg ttcgagagcg gcggcctggc
      301 cgccgcccac gccgtccaca acggcctgac cgcgctgccc cagctccacc acttcatgca
      361 cggggagaag gtcaacttcg gcaccctgac ccagttcgcc ctcgaggacc ggccgacccg
      421 cgagttcaac gagttcgtgt cgttctgcat gagcgtcggc ctgcccacca ccttcgccga
      481 cctcgacctg ggcacggtca gcagggagga gctggagacg gtggccaagg cggccaccgc
      541 ctccgccgag accatccaca acatgccgtt cgcggtcacg cccgggatgg tggtcgacgc
      601 catggtcgcc gccgacgcct attcgcgcgc ctaccagcag gagtcgggcc agcagccggc
      661 ggcttggagt cggccttcgg ccgacggcca ttagccttcg accggctccg ccggtcgact
      721 ggtgcgatca tgccccgatg ggagcggaag ccatcggggt cgggctggcc gggtacgggc
      781 tgggcgggtc ggtgttccgt gcgccgctag tccaggccgc gcccggcctg cgcctgcacg
      841 ccgtcgccac cagccgcgcc ggccaggtcg gccgggacct tcccggggtg cgggtggtgg
      901 cctcggccga ggagctgctg gaggacccgg cggtcgagct ggtggtggtg gcggcgccca
      961 acgccgtcca ccaccggctg gccgcggccg ccctgcgggc cgggcggcac gtggtcgtgg
     1021 acaagccgtt caccctgtcc acggccgagg ccgacgagct gatcgccctc gccgaggcgg
     1081 ccgaccggcg gctcagcgtg ttccacaacc ggcgctggga cggcgacttc ctcaccgtgc
     1141 ggcgctgcct cgaggccggc ctgctcgggg aggtgtccag cttcgtgtcc cgctacgacc
     1201 gcttccgccc cgtccccaag ggcggctgga aggaggaggc cgtccccggg tcgggcgtcc
     1261 tctgggacct ggggccgcac ctgatcgacc aggcgctgca gctgttcggc atgccggcga
     1321 cggtctgggc cgacctggcc gtccagcgtc ccggggtcga ggcggtcgac tacgtccacc
     1381 tggtgctcgg ctacgggcgg ctgcgggtcc tgctccacgc cgccatggag gtgcgccacc
     1441 ccgggccgcg cttcgaggtc cacggcgacc gggggtcgtt cgtcacctgg ggcatggaca
     1501 cccaggaggc ggccctgcgg gccggcgccc gccccggcga cccgggctgg gggaccgagc
     1561 cccccgaccg ccacggcacc ctcaccaccg aggtcgccgg cctcgggctc cacgcccggg
     1621 tgacggccgt gcccggcgac tacggggcct tctacgccgc catggccgcc gccgtcgccg
     1681 gcgagggccc ggtcccggtc gcgccctcgg aggcccgggc caccgtcgcc gtcaccgagc
     1741 acgccctccg cagcagccgc gagggccggg tggtcgaggt cgccgcggcg gcccccgcga
     1801 gctagccgga caggaacccg gccaggaacc gggtggaggc gggcaggacc tcctcggggg
     1861 cgccgcggag gcggcactcg aaggtgagcc agtcgtcgta gccgacgtcg cggagggcgg
     1921 cgaacacgct gccgaagtcc atgtggccgg tgccgggctg gagccggttg gtgtcgtcga
     1981 cgtggacgtg ggcgatgcgc gggccggcgg cccggatggc cgcggccagg tcgtcctcct
     2041 cgatgttcat gtggaacagg tcggcgcaga cccgcaccga gtcctgcccg gtcgcgcgct
     2101 cgacctcctc gccgagctcg accgcctggt cgaggcggtt gaccatgtag tcctcgtagc
     2161 ggttgatcgg ctccacggcc agccacaccc cctcggcggc ggcgtgcccg gccagctcga
     2221 ccagcgcctc cagcagcacc ttgtggtcct cgtcgggctg ccggggcggg gtgaagggcg
     2281 gcaggcgcag gctgaacatg ccccagctgg ccggggtcag cactccggcc cc
//
LOCUS       DASSWR010000079         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1133086, whole genome shotgun sequence.
ACCESSION   DASSWR010000079 DASSWR010000000
VERSION     DASSWR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133086"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFO47_01300"
     CDS             complement(<1..777)
                     /locus_tag="VFO47_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787367.1"
                     /translation="MAAISVQGLTKRFGDVLAVDQLDFGVDPGTVTGFLGPNGAGKTT
                     TLRMLLGLVAPTSGTATIGGRPYRELADPARRVGAVLEASGFHPGRTARDHLRVLATA
                     AGVAPGRVDEVLEQTGLAAAGRRRVGGFSLGMRQRLGLAAALLGDPEVLVLDEPANGL
                     DPEGVRWLRGLVRGLADQGRTVLVSSHALAEVAQTVDQVVIIDRGRLVAQSTLAALTA
                     GADRTVRVRTPQPEALRDLLVARGARATLDGPDRLVVAGAS"
     gene            complement(913..1578)
                     /locus_tag="VFO47_01305"
     CDS             complement(913..1578)
                     /locus_tag="VFO47_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8787368.1"
                     /translation="MIRVLVADDQTLVRAGFRVLVESAPDLEVVGEAGDGVEAVELAR
                     RDLPDVVLMDIRMPRMDGLEATRRIVALDQAAAVRVLVLTTFDLDEYVYQALRAGASG
                     FLLKDTPPADLLAAIRVVSAGDALLAPSVTRRLIAEFARRPEPSAVTPAALAALTDRE
                     REVLALVARGLSNAEIAEQLVVSAATAKTHVSRVLAKLQARDRAQLVMLAYETGLVTP
                     GSA"
     gene            complement(1575..2810)
                     /locus_tag="VFO47_01310"
     CDS             complement(1575..2810)
                     /locus_tag="VFO47_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET8787369.1"
                     /translation="MRDLDARRRRLPTPGPWALDGLLAVVAAGIGVATLATVLPFDPG
                     SPRALAAYLLLLAHTLPIAVRRRWPLPVLAWGLVTGAAFAALGLNLVSLSFAILVYVY
                     TAAAHCPRRRSLAGLAATEAMLLLIWLARPRLIGDTGTLVVDGLIVAAAWWLGDGTRR
                     RQEAVAEARRRAEELERAREELARRAVTEERLRIARELHDVVAHSMSIIAVQSGVGVH
                     VLDSQPEEARKALVAVEATSRQALVEMRRLLGVLRQEHEPRGSLAPAPGLAEVETLAA
                     EVARAGVRVEVRIEGTPAELPAGLDLSAYRIVQEALTNVLKHARPAAATVRVAYKPGQ
                     LVIEVTDDGRESASNGMESASGRPATDSDPAGQRPAGSGIAGMRERAALYGGTLEAGP
                     LPGGGFRVAATLPVEEVPG"
     gene            2957..>3257
                     /locus_tag="VFO47_01315"
     CDS             2957..>3257
                     /locus_tag="VFO47_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HET8787370.1"
                     /translation="MTEQPQQSQAPVPGVVVTGEPEQQQPTESIPSPAKVMRIGAMVR
                     QLLEEVRHAPLDEAGRDRMRDIYDISVRELSDSLSPDLAQELSRVARPFVGEETPS"
BASE COUNT          403 a         1249 c         1241 g          364 t
ORIGIN      
        1 ggaggccccg gcgaccacca gccggtccgg gccgtcgagg gtcgccctcg cgcccctggc
       61 gaccagcagg tcgcggaggg cctcgggctg cggggtgcgg acccgcaccg tccggtcggc
      121 cccggcggtc agggcggcca gggtcgactg ggcgaccagg cggccccggt cgatgatcac
      181 cacctggtcg accgtctggg cgacctcggc cagggcgtgg ctggacacga gcacggtccg
      241 gccctggtcg gccaggccgc ggacgaggcc gcgcagccag cgcacgccct cggggtcgag
      301 cccgttggcc ggctcgtcca gaaccaggac ctcggggtcg ccgagcaggg cggcggccag
      361 cccgaggcgc tggcgcatgc cgagcgagaa gcccccaacc cggcggcggc cggcggcggc
      421 cagcccggtc tgctccagga cctcgtcgac ccggcccggg gcgaccccgg ccgcggtggc
      481 caggacccgc aggtggtcgc gggccgtgcg gcccgggtgg aacccgctcg cctccagcac
      541 cgcgcccacc cggcgggccg ggtcggccag ctcgcggtag ggccgcccgc cgatggtggc
      601 cgtgcccgag gtcggggcga ccaggccgag gagcatccgc agggtggtcg tcttgccggc
      661 cccgttgggg ccgaggaagc cggtcaccgt cccggggtcg acgccgaagt cgagctggtc
      721 gacggcgagc acgtcgccga agcgcttggt gagtccctgc acgctgatgg ccgccatggc
      781 ctgcctcctt ggacgttcgg tgatgctggc gacgctacgg gccggggcgg cggccgtcgt
      841 cggcctggcg gccgcacctg gtggtcggcc tggaggcgga ggcgggaccg gtggtcggcc
      901 ccggggcgta ggtcaggcgg agccgggggt gaccaggccc gtctcgtagg cgagcatcac
      961 cagctgggcc cggtcgcggg cctggagctt ggccaggacc cggctgacgt gggtcttggc
     1021 cgtggccgcg ctcaccacca gctgctcggc gatctcggcg ttggagaggc cccgggcgac
     1081 cagggccagc acctcccgct cgcggtcggt gagggcggcc agcgcggccg gggtgaccgc
     1141 gctcggctcg gggcggcggg cgaactcggc gatcagccgc cgggtgacgc tgggggcgag
     1201 cagcgcgtcc ccagccgaca ccacccggat ggcggccagc aggtcggccg ggggcgtgtc
     1261 cttgaggagg aacccgctcg ccccggcccg cagcgcctgg tagacgtact cgtccaggtc
     1321 gaaggtggtg aggaccagga cgcgcacggc ggcggcctgg tcgagggcca cgatcctccg
     1381 ggtcgcctcg agcccgtcca tgcgcggcat gcggatgtcc atcaggacca cgtcgggcag
     1441 gtcccggcgg gccagctcga ccgcctccac cccgtccccg gcctccccga cgacctccag
     1501 gtcgggcgcc gactcgacca ggacccggaa tccggcccgg accagcgtct gatcgtccgc
     1561 caccaggacc cgtatcaccc gggcacctcc tccaccggca gggtggcggc gacccggaag
     1621 ccgccgccgg gcagcggccc ggcctccagg gtgccgccgt agagagcggc ccgctcgcgc
     1681 atcccggcga tgcccgaccc ggccggccgt tggccggccg ggtcggagtc tgttgctggt
     1741 cggccggagg ccgactccat gccgttagag gccgactccc tgccgtcgtc ggtgacctcg
     1801 atgaccaact gcccgggctt gtaggcgacc cggacggtgg ccgcagccgg gcgggcatgt
     1861 ttgagcacgt tggtcagcgc ctcctggacg atccggtagg ccgacaggtc cagcccggct
     1921 ggcagctcgg ccggggtccc ctcgatccgg acctcgaccc gcaccccggc cctggccacc
     1981 tcggcggcca gggtctccac ctcggccagc ccgggggccg gggcgaggct gccgcggggc
     2041 tcgtgttcct ggcgcagcac gccgagcagc cgccgcatct cgaccagggc ctggcggctg
     2101 gtcgcctcga ccgcgaccag ggccttgcgc gcctcctcgg gctggctgtc gagcacgtgg
     2161 accccgaccc cggactgcac ggcgatgatg ctcatgctgt gggcgaccac gtcgtgcagc
     2221 tcgcgggcga tccgcagccg ctcctcggtg accgcccgcc gggccagctc ctcgcgggcc
     2281 cgctccagct cctcggcccg ccgccgggcc tccgcgaccg cctcctggcg ccggcgggtg
     2341 ccgtcgccca gccaccaggc cgcggccacg atcaggccgt cgaccaccag cgtcccggtg
     2401 tcgccgatca accgcggcct ggccagccag atcaggagca gcatcgcctc ggtggcggcc
     2461 aggccggcca gcgagcggcg ccgcgggcag tgggcggcgg ccgtgtagac gtagaccagg
     2521 atcgcgaacg acagcgagac caggttgaga ccgagcgcgg cgaacgccgc ccccgtgacc
     2581 agcccccagg ccagcaccgg caggggccag cgccgccgca ccgcgatcgg cagggtgtgg
     2641 gcgagcagca gcaggtaggc cgccaaggcc ctcggcgatc cggggtcgaa gggcagcacc
     2701 gtggccaggg tggccacgcc gatgccggcc gccaccaccg ccagcaggcc gtcgagggcc
     2761 cacggcccgg gggtgggcag ccgtcgccgg cgggcgtcca ggtcgcgcat cgctgcaccg
     2821 taccgctccg cgcccctggc cgcgtcaccc tggaggccga cccgccgcct ccgcccggag
     2881 gtgtacgccg ggccggttcc cccggccgcg ggcgggacaa gtggcgaccg acgccgggct
     2941 gcgcgaagat gccggcatga ccgagcagcc acagcagtcc caggcccccg tccctggcgt
     3001 ggtggtgacg ggcgagccag agcagcagca gccgaccgag tcgatcccca gcccggccaa
     3061 ggtgatgcgc atcggcgcca tggtccgcca gctcctcgag gaggtccgcc acgcccccct
     3121 ggacgaggcc ggccgggacc ggatgcgcga catctacgac atctccgtgc gcgagctgtc
     3181 cgatagcctc tcgcccgacc tggcccagga gctgagccgg gtcgcccgcc cgttcgtcgg
     3241 cgaggagact cccagcg
//
LOCUS       DASSWR010000080         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_31450,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000080 DASSWR010000000
VERSION     DASSWR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31450"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFO47_01320"
     CDS             <1..440
                     /locus_tag="VFO47_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787371.1"
                     /translation="ADPDEQAARGLERFGIPQLARAMGTELSSGQKTLIGIVKSTMHD
                     PELLVLDEPTASLDPDVALRVRTGLATLCEERGTSLLVTSHNMVEVERIAERVVFLSA
                     GRVVADGPPFEVAARFGRDDLEEVFLHLASQRQASEHTGGLRP"
     gene            437..1264
                     /locus_tag="VFO47_01325"
     CDS             437..1264
                     /locus_tag="VFO47_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787372.1"
                     /translation="MTVMRVSRELAPWLRMWAVARRHAYVLLRSPHRLFDVTVWPIVD
                     TVLFGSLGVFFVRQGGPDSAAQAGVAYLLSGIVLWHVVYQAQIAVSTGFMEETWSRNL
                     LSLMVTPLRELELIAGIALFGLAKLVMGVGVVAMGAWALYAFDVTALGLAVLPVVAIL
                     MVGGWAIALFVVGLILRFGNGAEALAWGIMFVVMPLGGVFYPVEALPAFVQPISELLP
                     TTHAFAAGRELIDGGPVPWGELSLAAVGTLAATAAALAFVAWMLRLFRRRGYVTRYS"
     gene            complement(1261..>2074)
                     /locus_tag="VFO47_01330"
     CDS             complement(1261..>2074)
                     /locus_tag="VFO47_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8787373.1"
                     /translation="RVWALVNDIPRVAPCMPGAALTGVLDDNTYEGTVAVKLGPLRMS
                     YKGKVTVEEVDEANHSARLAASGRDTKGAGTARANVETRLESVDPTRTRLHVTSDVQL
                     TGKVASFGRGGAINDVATRLFGQFADCLRATLEAESTPAATGTAEAAGAGAAGTGAEA
                     GAGTEAGAGAEAAGVAGAGATTATGAQPGPSSDAVAAGDGTTTTGAGAAPTATSTTGT
                     PAPRPATPAPRAAEALDAGSLVGTMIKGRLQALFQAIGRFFRRLFGRGRSQA"
BASE COUNT          209 a          749 c          794 g          322 t
ORIGIN      
        1 tggccgaccc cgacgagcag gccgcccggg gccttgagcg cttcggcatc ccgcagctgg
       61 ccagggccat gggcaccgag ctgtcgtccg gccagaagac cctgatcggc atcgtcaagt
      121 ccaccatgca cgaccccgag ctgctggtcc tggacgagcc gacggccagc ctcgaccccg
      181 acgtggccct gcgggtccgc accggcctgg ccaccctgtg cgaggagcgg ggcacgtccc
      241 tgctggtcac cagccacaac atggtcgagg tggagcggat cgccgagcgg gtcgtgttcc
      301 tgtccgccgg ccgggtggtc gccgacggcc cgccgttcga ggtcgccgcc cgcttcggcc
      361 gcgacgacct cgaggaggtc ttcctccacc tggcctccca gcgccaggcc agcgagcaca
      421 ccggggggct gcggccatga ccgtcatgag ggtgtccagg gagctggcgc cgtggctgcg
      481 catgtgggcg gtggcccgcc ggcacgccta cgtgctgctc cgcagcccgc accggctgtt
      541 cgacgtgacc gtctggccga tcgtggacac ggtgctgttc gggtccctcg gggtgttctt
      601 cgtccgccag ggtgggccgg actcggccgc ccaggccggg gtggcctacc tgctgtccgg
      661 gatcgtcctc tggcacgtcg tctaccaggc tcagatcgcc gtctcgaccg gcttcatgga
      721 ggagacctgg tcgcgcaacc tgctcagcct gatggtgacg ccgctccggg agctggagct
      781 gatcgccggg atcgccctgt tcgggctggc caagctggtg atgggcgtcg gcgtggtggc
      841 catgggggcg tgggccctct acgccttcga cgtcaccgcc ctcgggctcg ccgtgctgcc
      901 ggtggtcgcc atcctgatgg tgggcgggtg ggcgatcgcc ctgttcgtgg tcggcctgat
      961 cctgcgcttc ggcaacgggg cggaggcgct ggcctggggc atcatgttcg tggtgatgcc
     1021 gctgggcggc gtcttctacc cggtcgaggc cctcccggcg ttcgtgcagc cgatctcgga
     1081 gctgctgccg accacccatg ccttcgccgc cggccgcgag ctgatcgacg gcggcccggt
     1141 cccgtggggc gagctctccc tggccgccgt cggcaccctg gccgccaccg ccgccgccct
     1201 cgccttcgtc gcctggatgc tgcgcctgtt ccgccgccgc gggtacgtga cccgctacag
     1261 ctaggcctgg gaccggccgc ggccgaacag gcggcggaag aagcggccga tggcctggaa
     1321 cagggcctgc aggcggccct tgatcatggt gccgaccagg ctgccggcgt cgagggcctc
     1381 ggccgcccgg ggcgccgggg tggcgggtcg gggggccggg gtgccggtcg tggacgttgc
     1441 cgtgggagcg gcgccggcgc cggtggtggt cgtcccgtcg cccgcggcga ctgcatccga
     1501 cgaggggccg ggctgggccc cggttgcggt ggtggccccc gcgcccgcca cgcccgccgc
     1561 ctctgcgccc gcgcccgcct ctgtgcccgc gcccgcctcg gcccccgtgc cggccgcccc
     1621 cgcacccgcc gcctccgccg taccggtggc cgccggggtc gattcggcct ccagggtggc
     1681 gcggaggcag tcggcgaact ggccgaagag gcgggtggcg acgtcgttga tggcaccgcc
     1741 gcggccgaag gaggcgacct tgccggtgag ctggacgtcg ctggtgacgt ggaggcgggt
     1801 gcgggtgggg tcgacggatt cgaggcgggt ctcgacgttg gcgcgggcgg tgccggcgcc
     1861 tttggtgtcg cggccgctgg cggccaggcg ggcgctgtgg ttggcctcgt cgacctcctc
     1921 gaccgtgacc ttgcccttgt aggacatgcg cagcgggccg agcttgaccg ccaccgtgcc
     1981 ctcgtaggtg ttgtcgtcca gcacgccggt cagggccgcc cccggcatgc acggggcgac
     2041 ccgcgggatg tcgttgacca gggcccagac ccgg
//
LOCUS       DASSWR010000081         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_370993,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000081 DASSWR010000000
VERSION     DASSWR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370993"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFO47_01335"
     CDS             <1..235
                     /locus_tag="VFO47_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET8787374.1"
                     /translation="AMVAAVLGDTGEVMPVCAWVTGEYGIDGVYLGVPARLGRGGVTE
                     VVELPLTGEELADLREAAEAVRSKQADVAKLVG"
     gene            228..554
                     /locus_tag="VFO47_01340"
     CDS             228..554
                     /locus_tag="VFO47_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787375.1"
                     /translation="MADQAHRDPAAGAASGCALCAAEPLTPWFHEDDRCWVAECLICR
                     TPMVVWRHHGSDPPPSDREHMLTVLSGVAAARLGPSGFRIDPVMRRIPDHFHAHARDP
                     NWPGFA"
     gene            773..>2190
                     /locus_tag="VFO47_01345"
     CDS             773..>2190
                     /locus_tag="VFO47_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET8787376.1"
                     /translation="MAGNSFGAKAGLDVGDRSFEVFRLDALGSVGDVGRLPFSLKVLL
                     ENLLRNEDGSAVTAEDVEAFASWDPTSAEDREIAFSPARVLMQDFTGVPAIVDLAAMR
                     DAMAALGGDASRINPLAPAELVIDHSVVADIAGRPDAFVRNVELEFERNRERYAFLRW
                     GQQAFADFKVVPPGTGIVHQINCEYLARVVFTNDRGQAYPDTLVGTDSHTPMVNGLGV
                     LGWGVGGIEAEAAMLGQPISMLMPKVVGFKLSGELPEGSTATDLVLTVTELLRRHGVV
                     GKFVEFYGPGVGNVPVENRATIGNMSPEYGSTCAIFPIDAETLRYLEFTGRPPELIAL
                     VEAYAREQGLWHDPDAEPRYSETVELDLGTVVPSLAGPRRPQDRVALTDARRGFRAAL
                     ADMTPEGTSQTRQDTPIDRKVDEDGPDEASAESFPASDPPAAMAGTMAHENHDPPPAG
                     WFEPDPAGASGRPGKRVPVTLE"
BASE COUNT          295 a          815 c          800 g          280 t
ORIGIN      
        1 ggccatggtg gcggccgtcc tcggcgacac cggcgaggtc atgccggtct gcgcctgggt
       61 caccggcgag tacggcatcg acggcgtcta cctgggcgtc ccggccaggc tgggtcgcgg
      121 cggggtcacc gaggtcgtcg agctgccgct gaccggcgag gagctggccg acctgcgcga
      181 ggccgccgag gcggtccgtt ccaagcaggc cgacgtggcc aagctggttg gctgaccagg
      241 cccaccggga cccggccgcc ggggccgcct ccggctgtgc cctgtgcgcg gccgagccgc
      301 tgaccccctg gttccacgag gacgatcgct gctgggtcgc ggaatgcctg atctgccgca
      361 ccccgatggt cgtctggcgc caccacggct ccgacccgcc gccctccgac cgcgagcaca
      421 tgctgaccgt cctctccggc gtggcggcgg cccgcctcgg cccgtccggg ttccgcatcg
      481 acccggtgat gcgccgcatc cccgaccact tccacgccca cgcccgcgac cccaactggc
      541 ccgggttcgc ctgagcggta ggtggcgtcg ggggtgaaca ggccgaggac ggccggggtg
      601 tcggcctcct cccaggcccg ccggtaggcg gccagccaga cgccggccgg ttcgcggtcc
      661 atgaccgccg atcgcagggc cgggggggtg accggaccgc cgcctgaagc gtcccctcac
      721 cgggtaccct gcagagcgcg aacctgacag gcagatgcgg agggccggga ccatggcggg
      781 gaacagcttc ggggccaagg cggggttgga cgtcggggac cggtcgttcg aggtcttccg
      841 gctggatgcg ctcgggtcgg tgggggacgt cgggaggctg ccgttctcgc tcaaggtgct
      901 gctcgagaac ctgctccgga acgaggacgg gagcgccgtc accgccgagg acgttgaggc
      961 gttcgcgagc tgggacccga cgtcggccga ggaccgggag atcgcgttct cgccggcgcg
     1021 ggtgctgatg caggacttca ccggcgtgcc cgcgatcgtc gacctggccg ccatgcgcga
     1081 cgccatggcc gccctcggcg gcgacgcgag ccggatcaac ccgctggccc cggccgagct
     1141 ggtcatcgac cactcggtgg tggccgacat cgccggccgg cccgacgcct tcgtgcgcaa
     1201 cgtggagctg gagttcgagc gcaaccggga gcggtacgcg ttcctgcgct ggggccagca
     1261 ggccttcgcc gacttcaagg tcgtgccccc cggcaccggc atcgtccacc agatcaactg
     1321 cgagtacctg gcccgggtgg tgttcaccaa cgaccgcggc caggcctacc ccgacaccct
     1381 ggtcggcacc gacagccaca ccccgatggt caacggcctg ggcgtgctcg gctggggcgt
     1441 cggcggcatc gaggccgagg cggccatgct cggccagccg atctcgatgc tcatgcccaa
     1501 ggtggtcggg ttcaagctgt cgggcgagct gcccgagggc tcgaccgcca ccgacctggt
     1561 cctgaccgtg accgagctgc tgcgccgcca cggggtggtg ggcaagttcg tcgagttcta
     1621 cggccccggg gtcggcaacg tgccggtcga gaaccgggcc accatcggca acatgtcgcc
     1681 cgagtacggg tcgacctgcg ccatcttccc gatcgacgcc gagaccctgc gctacctcga
     1741 gttcaccggc cgcccgcccg agctgatcgc cctggtcgag gcgtacgcca gggagcaggg
     1801 cctgtggcac gacccggacg ccgagccccg ctactccgag acggtcgagc tggacctggg
     1861 cacggtggtg ccgagcctgg ccgggccgcg ccggccccag gaccgggtcg ccctcaccga
     1921 cgcccgccgc ggcttccggg ccgccctggc cgacatgacc ccggagggaa cctcccagac
     1981 caggcaggac accccgatcg accgcaaggt cgacgaggac gggccggacg aggcctcggc
     2041 cgagtcgttc ccggccagcg acccgccggc ggccatggcc ggcacgatgg cccacgagaa
     2101 ccacgacccc ccgccggccg gctggttcga gcccgacccg gccggggcca gcggccggcc
     2161 cggcaagcgg gtcccggtca ccctggagga
//
LOCUS       DASSWR010000082         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_173355,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000082 DASSWR010000000
VERSION     DASSWR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173355"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFO47_01350"
     CDS             <1..662
                     /locus_tag="VFO47_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8787377.1"
                     /translation="SQIIVPAATPGVEIVRAIPVMGHRGRGWSTHCEVRLTGVRVPVA
                     NTIGEPGDGFRIAQKRLGPGRIHHVMRWLGQMQRAFELMCGYALRRQAFGGPLATKQT
                     VQTWVADSAAEIQACRLLTLDAAARIDQGEEARVEISAVKFYAARVLHDVIDRAVQVH
                     GALGLTDQTPLARMYADARAARLYDGPDEVHRMVVSRRVLRAFEQGAVWDFAQGGPRL
                     V"
     gene            694..1215
                     /locus_tag="VFO47_01355"
     CDS             694..1215
                     /locus_tag="VFO47_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787378.1"
                     /translation="MPPEQDDDRERQLAALRRYVSDLDMRRQHQGQARVVEERRRPAW
                     PWLLLTLVLMAVSLAGGVAIGANRDAVGDPARYSGTTATSAAPSPAASPECAEAVVKA
                     NRTLSHAVRVSGALREHTEAMNKLLNGELGTKEAVQAGTPSLISGSAESARFDVALAD
                     YRKVVDRCQLQVP"
     gene            complement(1224..>2412)
                     /locus_tag="VFO47_01360"
     CDS             complement(1224..>2412)
                     /locus_tag="VFO47_01360"
                     /inference="COORDINATES: protein motif:HMM:NF037017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET8787379.1"
                     /translation="VTAVAVVLALVGGGVVALAGRDGGGPGTGSGEQRAGADGGPAQA
                     APVRLTVTPRNKATRVALDAKAKVVAESGRLRSVRVTGAGKRVDGELAEDGRSWESSG
                     PLAPATRYRVVAEAVDDAGTPTRRQSSFTTLRPRAELRAAVMPLDGETVGVGLPIGVW
                     FSQPVADRAAVERRLEVSSSKPVTGAWHWFADNEVHYRPKAYWPSGSKVTLRARLAGT
                     DAGRGVWGVADRTIRFTIGQRRVSVVDVRTHRMKVTSAGKTLRVLPVSTGRERYPTTN
                     GVHFVIEKSAVKLMDSSTVGIPRNSPGGYYQRVNWSVRISNSGEFVHAAPWSTGAQGS
                     ANVSHGCVNLSTANAAWYFRLTRRGDVVEVRGSPKRPGSSLGVADWNMSWSKWLAGSA
                     LPR"
BASE COUNT          306 a          908 c          889 g          309 t
ORIGIN      
        1 tgagccagat catcgtcccg gcggccaccc ccggggtcga gatcgtccgc gccatcccgg
       61 tcatgggcca ccggggccgc ggctggagca cccactgcga ggtccgcctc accggcgtcc
      121 gggtcccggt ggccaacacc atcggtgagc ccggggacgg cttccgcatc gcccagaagc
      181 ggctcggccc cggccgcatc caccacgtca tgcgctggct ggggcagatg cagcgagcct
      241 tcgagctgat gtgcggctac gcgctccgcc gccaggcgtt cggcgggccg ctggccacca
      301 agcagacggt gcagacctgg gtggccgact cggcggccga gatccaggcc tgccggctgc
      361 tcaccctgga cgccgcggcc cgcatcgacc agggggagga ggccagggtc gagatctcgg
      421 cggtcaagtt ctacgccgcc cgggtgctgc acgacgtgat cgaccgggcg gtccaggtcc
      481 acggcgccct cggcctgacc gaccagaccc cgctggcccg gatgtacgcc gacgcccggg
      541 cggcccgcct ctacgacggc cccgacgagg tccaccgcat ggtcgtgagc cgccgggtcc
      601 tgcgggcgtt cgagcagggc gcggtctggg atttcgccca gggcggcccc cgtttggtat
      661 gacatggcaa ggttcgcccg ggagggggaa gccgtgcctc cagagcaaga cgacgaccgc
      721 gagcgccagc tcgccgccct gcggcgctac gtctccgacc tcgacatgcg ccgccagcac
      781 cagggccagg cgcgggtggt cgaggagcgg cgccggcccg cctggccctg gctgctgctc
      841 accctggtcc tgatggccgt gtccctggcc ggcggggtgg ccatcggcgc caaccgcgac
      901 gccgtcggcg acccggcccg ctactcgggg accaccgcga ccagcgccgc cccctccccc
      961 gccgcctccc ccgagtgcgc cgaggcggtg gtgaaggcca accggaccct gagccacgcc
     1021 gtccgggtgt ctggcgccct gcgcgagcac accgaggcca tgaacaagct gctcaacggc
     1081 gagctcggca ccaaggaggc ggtgcaggcc ggcaccccgt ccctgatttc cggctcggcc
     1141 gagtcggcca ggttcgacgt tgccctggcc gactaccgca aggtggtcga ccgctgccag
     1201 ctgcaggtgc cctgaccggg cggtcaccgc ggcagggcgc tgccggccag ccacttcgac
     1261 caggacatgt tccagtcggc aaccccgagg ctgctgcccg ggcgcttggg cgagcccctg
     1321 acctcgacca cgtcgccgcg gcgggtcagg cggaagtacc aggcggcatt ggcggtggag
     1381 aggttgacgc agccgtggga gacgttggcc gagccctggg cgccggtcga ccagggggcg
     1441 gcgtggacga actcgccgga gttggagatc cgcaccgacc agttgacccg ctggtagtag
     1501 ccgccggggg agttcctggg gatgcccacg gtggaggagt ccatcagctt gacggcgctc
     1561 ttctcgatga cgaagtggac gccgttggtg gtcgggtagc gctcgcggcc ggtggagacc
     1621 ggcaggaccc gcagggtctt gccggccgag gtgaccttca tgcggtgggt ccggacgtcg
     1681 accacgctga cccggcgctg gccgatggtg aagcggatgg tgcggtcggc cacaccccag
     1741 accccgcggc cggcgtcggt cccggccagg cgggcccgca gggtgacctt ggacccgctc
     1801 ggccagtagg ccttggggcg gtagtggacc tcgttgtcgg cgaaccagtg ccaggccccg
     1861 gtgaccggct tggacgagct gacctccagg cgccgctcga ccgcggcccg gtcggccacc
     1921 ggctggctga accagacgcc gatgggcagg ccgaccccga ccgtctcgcc gtccaggggc
     1981 atgaccgcgg cccgcagctc ggcccggggc ctgagggtgg tgaagctgct ctggcggcgg
     2041 gtcggagtgc cggcgtcgtc gaccgcctcg gccaccaccc ggtagcgggt cgccggggcc
     2101 agggggccgc tgctctccca gctgcgcccg tcctccgcga gctccccgtc cacgcgcttg
     2161 ccggccccgg tcacccggac cgaccgcagc cgcccggact cggccacgac tttggccttg
     2221 gcgtcgagtg cgacccgggt cgccttgttc ctcggggtca cggtgaggcg caccggcgcc
     2281 gcctgggccg gcccgccgtc ggccccggcc cgctgctcgc ccgacccggt gccgggcccg
     2341 ccgccgtccc tgccggccag cgcgaccacg ccgccgccga ccagggcgag gacgacggcc
     2401 acggcggtca ct
//
LOCUS       DASSWR010000083         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_653764,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000083 DASSWR010000000
VERSION     DASSWR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653764"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFO47_01365"
     CDS             complement(<1..1140)
                     /locus_tag="VFO47_01365"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET8787380.1"
                     /translation="MKERVEELRTRKEQALHAGSPKAVEVQHDKGKLTARERIDGLLD
                     PGSFVETDMLAVHRATAFGMDKRRIPGDGVVTGYGTIGGRRVCLFSQDFTVFGGSLGE
                     VMAEKICKVMDLSLKMGVPCIGINDSGGARIQEGVVALSGYAEIFWRNVQASGVVPQL
                     SLIMGPSAGGAVYSPAITDFVLMVKETSYMFITGPDVVKTVTGEDVTFEELGGAMTHA
                     TKSGTAHFAAEDEDECFDLARELVGFLPLNNMEDPPRRQPTDDPRREEQALVDLVPDH
                     PNQPYDIREVVTRVVDDGEFLEILQAHAESMVIGLARLNGRPVGIVGNQPLHLAGVLD
                     INASVKGARFVRFCDAFNIPILSFVDVPGFMPGTSQEWGGIIRHGA"
     gene            complement(1250..>2088)
                     /locus_tag="VFO47_01370"
     CDS             complement(1250..>2088)
                     /locus_tag="VFO47_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8787381.1"
                     /translation="RLSRPLGGMVRNASIAKDALGWHVSFGVAIAAKPAPPNRLPSCG
                     VDFGVACSAFVSDEREPRLMPSTLTAGQRRRLLGLERRKSRQITWAKKHNDGHYSNRL
                     GRTISEISKLRARQARRRQDFIHKLTTDLAKNHGWVAVEDLRVKGMTRSAKGTREEPG
                     RNVKAKASLNRTILDNAPYERQRQLAYKAPRFGSELRLVRPAFTSQTCSACGLRDPNS
                     RPGCGRLFACTVCGHVEHADLNAARNIHNLAAGQAVHSTRSHPRAARPSSRMREPLGS
                     IA"
BASE COUNT          292 a          699 c          740 g          357 t
ORIGIN      
        1 ggccccgtga cggatgatgc cgccccactc ctggctggtg ccgggcatga acccgggcac
       61 gtcgacgaag ctgaggatgg ggatgttgaa ggcgtcgcag aagcgcacga accgggcgcc
      121 cttgaccgag gcgttgatgt cgaggacccc ggccaggtgc agcggctggt tgccgacgat
      181 cccgaccggc cggccgttga gccgggccag gccgatcacc atcgactcgg cgtgggcctg
      241 gaggatctcc aggaactcgc cgtcgtcgac cacccgggtg accacctcgc ggatgtcgta
      301 gggctggttg gggtggtcgg ggaccaggtc gaccagggcc tgctcctccc gccgggggtc
      361 gtcggtgggc tggcgccggg gcgggtcctc catgttgttg aggggcagga acccgaccag
      421 ctcccgggcc aggtcgaagc actcgtcctc gtcctcggcg gcgaagtggg ccgtgcccga
      481 cttggtggcg tgggtcatgg ccccgcccag ctcctcgaag gtcacgtcct cgccggtgac
      541 cgtcttcacc acgtccgggc cggtgatgaa catgtaggag gtctccttga ccatcagcac
      601 gaagtcggtg atggccgggg agtagacggc gccgccggcc gaggggccca tgatcagcga
      661 cagctgcggg accaccccgg aggcctggac gttgcgccag aagatctcgg cgtagccgga
      721 cagggcgacc acgccctcct ggatgcgggc cccgccggag tcgttgatgc cgatgcaggg
      781 cacccccatc ttcagcgaca ggtccatgac cttgcagatc ttctcggcca tgacctcgcc
      841 gagggagccg ccgaacacgg tgaagtcctg ggagaacagg cagacccggc ggccgccgat
      901 ggtgccgtag ccggtgacga ccccgtcccc ggggatgcgc cgcttgtcca tgccgaaggc
      961 ggtcgcccgg tgcacggcca gcatgtcggt ctccacgaac gagcccgggt cgagcaggcc
     1021 gtcgatccgt tcgcgggcgg tgagcttgcc cttgtcgtgc tggacctcga ccgccttggg
     1081 ggagccggcg tgcagcgcct gctccttgcg ggtgcggagc tcctccaccc gttccttcat
     1141 ggtcttcggc tgctgtggcg ccacctggcc atcctcccca cccgcgcgcg ctggtcgcag
     1201 gcattttgac atcctcccct gcctgaaggg cggggattcc cgcgagtgct caggcgatac
     1261 tcccgagtgg ttcacgcatc cggctcgatg gcctcgccgc ccgagggtga ctgcgcgtgc
     1321 tgtggaccgc ctgcccggcg gcaagattgt ggatgtttcg tgcggcgttc aggtccgcat
     1381 gctcgacatg gccgcagacg gtgcaggcga acagccggcc gcagcccggc cgtgagttcg
     1441 ggtcacggag cccacacgcc gagcatgtct ggcttgtgaa cgccggccga acgagtcgca
     1501 gctctgaccc aaatctgggt gccttgtacg cgagctggcg ctgccgctcg tacggggcgt
     1561 tgtcgaggat ggtccggttc aggctagctt tggccttgac gttcctgccg ggctcctccc
     1621 tcgtgccctt ggccgacctt gtcatgccct tcacccgcag gtcctcgacg gcaacccagc
     1681 cgtggttctt ggcgaggtcg gtggtgagct tgtggatgaa gtcctgccga cggcgcgcct
     1741 ggcgcgcccg aagcttggag atctcgctga tggtccggcc cagccggttg gagtagtgac
     1801 catcgttgtg cttcttggcc caggtgatct ggcggctctt gcggcgctcc agccccagca
     1861 ggcgtcgtct ctgtcccgcg gtgagggtcg atggcatcag gcgcggttcc cgctcgtcgg
     1921 acacgaacgc cgagcaggcc accccgaagt ccactccaca gctcggcagg cggttcggcg
     1981 gagcgggttt ggcggcgatc gcaacaccga acgagacatg ccagccgagt gcgtccttgg
     2041 cgatcgaggc gttgcgcacc atgcccccga gggggcggga cagccgga
//
LOCUS       DASSWR010000084         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_427728,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000084 DASSWR010000000
VERSION     DASSWR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427728"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFO47_01375"
     CDS             complement(<1..495)
                     /locus_tag="VFO47_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET8787382.1"
                     /translation="MSTTPAPLSWSGVTARRMARHALTEPASDLGPAEVAGVLCGAHA
                     QVLSAAELSIGRRIAGATRADVQRALWEDRTLVKTFGPRGTVHLLAAVDLSMWTGALS
                     ALPSSVPAHPEPVRFSAEQAEEVIAAIGDALADTELTVDELTEAIVDRTGPWAGERTM
                     AAFQD"
     gene            complement(632..>2065)
                     /locus_tag="VFO47_01380"
     CDS             complement(632..>2065)
                     /locus_tag="VFO47_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET8787383.1"
                     /translation="DALRRRAPKRLAVQAYVDGNLLRKEAAFRARWRYRGRIEHVGHH
                     DAHAASAFFPSPFERAAVLTLDRGGDFLSTTLQVGTGSRLHRLAQVRNPDSLGEVYSA
                     LTWYLGFEPNADEGKTMGLAPYGRDKLAGELADLVSRTPDGLFRVNLAWFAYHREGGW
                     LSRQFLERFGPPRVPESAITETHQDLAFAVQDLLEETALHVARALRRGSGSPNLCLAG
                     GVALNSVMNSRLLREAGFERIFIQPAASDAGNALGAALWVWHQRLGRSRAEPMTHAFH
                     GAEWSEPEQAEVLTGQGLAVQTVTDPAEEAARLLAEGRIVGWFQGRAEVGPRALGARS
                     ILADPRHAETKDVVNARVKRREAFRPFAPSVLDEHGAEFFEGYYPNPFMLLVQPVRPD
                     RRAQIPAVTHVDGTARLQTVTATENPLYHRLISRFAARTGVPVVLNTSFNLRGEPIVH
                     RPAEAVADFLASDMDALFLGNLLATKT"
BASE COUNT          299 a          720 c          765 g          281 t
ORIGIN      
        1 gtcctggaac gccgccatgg tccgctcgcc ggcccacggg ccggtgcggt ccacgatcgc
       61 ctcggtcagc tcgtcgacgg tcagctcggt gtcggccagg gcgtcgccga tggccgcgat
      121 gacctcctcg gcctgctcgg cgctgaagcg gaccggctcg ggatgggccg gcaccgacga
      181 cggcagggcc gacagcgccc ccgtccacat cgacaggtcg acggccgcca acaggtgcac
      241 ggtgccgcgc ggaccaaacg tcttgaccag agtgcggtcc tcccacaggg cgcgctggac
      301 gtcggcgcgg gtcgccccgg cgatgcgccg cccaatcgag agctcggcgg cgctgaggac
      361 ctgggcgtgg gctccgcaca gcacgccggc gacctcggcc gggcccaggt ctgacgccgg
      421 ttcggtcaag gcgtggcggg ccatccgccg ggccgtgacg ccgctccagc tcagcggggc
      481 cggggtcgtg gacatggggg tcacggaccg cattctcccg gctcgcccga cagcggtcac
      541 gtcgaggacg gtggcctggc acacgcccag actacctgac cgtggagacc agaagatgcg
      601 ggtcagagtt catctttccc gggtcggccc gctaggtctt tgtggccagc aggttgccga
      661 ggaacagggc gtccatgtca ctggctagga agtcggccac ggcttcggcc gggcggtgga
      721 caatcggctc gccgcggagg ttgaagctgg tgttgaggac caccgggacg ccggtgcggg
      781 cggcaaagcg gctgatcagc cggtgataca ggggattctc ggtggccgtg accgtctgga
      841 gccgggcggt gccgtccaca tgagtgacgg ccgggatctg ggcccgtcgg tcgggccgca
      901 ccggctgcac caggagcatg aaggggtttg ggtagtagcc ctcgaagaac tcggcgccgt
      961 gttcgtccaa cacgctgggg gcgaacggac ggaacgcctc ccggcgcttg acccgggcgt
     1021 tgacgacgtc cttggtctcg gcgtggcggg gatcggccag gatcgagcgg gcccccagcg
     1081 cccgcggccc gacctcggcc cggccctgga accagcccac gatgcggccc tcggccagca
     1141 ggcgggccgc ctcctcggcc gggtcggtca ccgtctggac cgccagcccc tggccagtca
     1201 gcacctcggc ctgctctggc tcgctccact cagccccgtg gaaggcgtgg gtcatcggct
     1261 cggcgcggga ccggccgagc cgctggtgcc agacccacag agccgccccc agggcgttgc
     1321 cggcgtcgga ggcggccggc tggatgaaga tccgctcgaa gccggcctcg cgcagcagcc
     1381 ggctgttcat caccgagttc agggcaaccc cgccggccag gcacagattt ggcgagccgc
     1441 tgccccgccg cagggcgcgg gcgacgtgca aggccgtctc ctccagcagg tcctgcaccg
     1501 caaacgcgag gtcctggtgg gtctcggtga tggccgactc gggcacccga ggtggtccga
     1561 accgctccag gaactggcgt gacagccagc cgccctcgcg gtggtaggcg aaccaggcca
     1621 ggttgacccg gaacagcccg tccggggtcc gggagaccag gtcggcaagc tcgccggcaa
     1681 gcttgtcgcg gccatagggg gcaaggccca tggtcttgcc ctcatcggcg ttgggctcga
     1741 agcccagata ccaggtgagg gctgagtaca cctcgcccag ggagtctggg ttgcgaacct
     1801 gggccagccg gtgaagccgc gacccggtgc cgacctgcag ggtggtcgac aggaaatccc
     1861 caccccggtc cagggtaagc accgccgccc gctcgaatgg gctggggaag aaggcgctgg
     1921 cggcatgcgc gtcgtggtga ccgacgtgct cgatccgccc ccggtagcgc cagcgggccc
     1981 ggaaggcggc ctccttgcgc agcaggttgc catcgacata ggcctgcacg gccagccgtt
     2041 tgggcgcccg ccgccggagc gcgtc
//
LOCUS       DASSWR010000085         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_483793,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000085 DASSWR010000000
VERSION     DASSWR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483793"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFO47_01385"
     CDS             <1..903
                     /locus_tag="VFO47_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8787384.1"
                     /translation="AGMPCISTVTPYQPWYLARSPRPNPAKPKPYQWTWHFFWGLEDI
                     IGVFADMWSQVDNNHVVGGLWPNDGDGRAWSVGFPKVLRNQAYEIVDPGRYQNEKNSF
                     AAEIEKFKAEGAEIVTGVPLPPDFATFWLQAADRGYRPKIASVGKALLFPSFIEAMGS
                     SAEGVSTEVWWSPRHPFTSSLTGASAGQLATDYQNQTGRQWIQPIGFVHALFEVAASV
                     LQRAGDVGDKQALADAIKATSMRTIVGTVDWTRTQADFPNVSRTPLVGGQWRRGGSRP
                     FELEIVSNSVHPEIPATSRLQPIS"
     gene            969..2228
                     /locus_tag="VFO47_01390"
     CDS             969..2228
                     /locus_tag="VFO47_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8787385.1"
                     /translation="MQLAGLSGAAVLAGCRQSDSGSDAARTLKIGYVSPKTGPLAGFG
                     EADDFILAGVRKALEGGLEIGGTSYPVEILTKDSQSDPNRAATVAGDLINADQIDLML
                     VASTPETTNPVADQCEANGVPCISSVAPWQPWFFGRQGDPEKPFQWTYHFFWGLEDII
                     AVFTDMWGQVDNNKAVAALWPNDGDGNAWGDKKLGFPPALTKEGYKIVDPGRYQNGTE
                     DFSSQIAEFKAAKAEIVTGVPIPPDWTTFWKQASQQGFQPKIASVGKALLFPASVEAL
                     GDIGDGMSTEVWWSPAHPYTSSLTGASAAKVAEEYQASTGRQWTQPIGFVHALFEVAA
                     AALKASGDVGDRQALANAIKGVDLDTVVGKISWSGGPVPNVAKTPLVGGQWRKSDGKY
                     PFELVIVSNKDHPDIPKGGSLEPIPYA"
     gene            2239..>2638
                     /locus_tag="VFO47_01395"
     CDS             2239..>2638
                     /locus_tag="VFO47_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8787386.1"
                     /translation="MAGRALELDGLSKSFGALKVIDDLHVHLDEGEALGVVGPNGAGK
                     TTMLHLAAGQLRPDAGRVRLAGQDVTGLPARARCQLGLARTFQVPQPFSGMTVYENVL
                     VGATFGAGGRGGSSLAPRRVAHPDAHRASLE"
BASE COUNT          425 a          983 c          881 g          349 t
ORIGIN      
        1 gccgggatgc cctgcatctc caccgtcacc ccctaccagc cctggtacct ggcccggagc
       61 ccgcgtccca accccgccaa gcccaagccc taccagtgga cctggcattt cttctggggc
      121 ttggaggaca tcatcggcgt gttcgccgac atgtggagcc aggtcgacaa caaccacgtc
      181 gtgggcgggc tgtggcccaa cgacggcgac ggccgggcct ggagcgtcgg cttccccaag
      241 gtgctccgca accaggccta cgagatcgtc gaccccggcc gctaccagaa cgagaagaac
      301 agcttcgccg ccgagatcga gaagttcaag gccgaagggg ccgagatcgt caccggagtg
      361 ccgctgcccc ccgacttcgc cacgttctgg ctgcaggcgg ccgaccgggg ctaccggccc
      421 aagatcgcct cggtcggcaa ggcgctgctg ttcccctcct tcatcgaggc catgggcagc
      481 agcgccgagg gcgtctcgac cgaggtctgg tggagccccc ggcacccgtt cacctcctcg
      541 ctgaccgggg ccagcgccgg ccagctggcc accgactacc agaaccagac gggccgccag
      601 tggatccagc ccatcggctt cgtccacgcc ctgttcgagg tggcggcgtc ggtgctccag
      661 cgggccggcg acgtcggcga caagcaggcc ctggccgacg ccatcaaggc gaccagcatg
      721 cgcacgatcg tcggcaccgt cgactggacc cgcacccagg ccgacttccc caacgtctcc
      781 cggaccccgc tggtcggggg ccagtggcgc cggggcggca gccggccgtt cgagctcgag
      841 atcgtctcca acagcgtcca ccccgagatc ccggctacca gcaggttgca gccgatcagc
      901 tgacggctgc ggtgaccacc aggaggtcgc tcgtgagcag tggcagaggc gtcgatcgcc
      961 gccggttcat gcagctcgcc gggctctcag gagccgcggt cctcgccggc tgcaggcagt
     1021 cggactcggg ctccgacgcc gcccggaccc tcaagatcgg ctacgtctcc cccaagaccg
     1081 gccccctggc cggcttcggc gaggccgacg acttcatcct cgcgggcgtg cgcaaggcgc
     1141 tggagggcgg cctggagatc ggcggcacgt cgtacccggt ggagatcctc accaaggaca
     1201 gccagtccga ccccaaccgg gcggccacgg tcgccggcga cctgatcaac gccgaccaga
     1261 tcgacctgat gctggtcgcc tccaccccgg agacgaccaa cccggtggcc gaccagtgcg
     1321 aggccaacgg cgtcccctgc atctccagcg tcgccccctg gcagccgtgg ttcttcggcc
     1381 gccagggaga cccggagaag ccgttccagt ggacctacca cttcttctgg ggcctcgagg
     1441 acatcatcgc cgtgttcacc gacatgtggg gccaggtgga caacaacaag gcggtggccg
     1501 cgctgtggcc caacgacggc gacggcaacg cctggggcga caagaagctg ggcttcccgc
     1561 ccgccctcac caaggagggc tacaagatcg tcgacccagg ccgctaccag aacggcaccg
     1621 aggacttctc ctcccagatc gccgagttca aggcggccaa ggccgagatc gtcaccggcg
     1681 tgcccatccc cccggactgg accaccttct ggaagcaggc ctcccagcag gggttccagc
     1741 ccaagatcgc ctcggtcggc aaggcgctgc tgttcccggc ctcggtcgag gcccttggcg
     1801 acatcggcga cggcatgtcc accgaggtct ggtggagccc ggcccacccc tacacctcct
     1861 cgctcaccgg ggccagcgcg gccaaggtgg ccgaggagta ccaggcgtcc acgggcaggc
     1921 agtggaccca gcccatcggc ttcgtccacg ccctgttcga ggtggccgcg gccgccctca
     1981 aggcgagcgg cgacgtcggc gacaggcagg ccctggccaa cgccatcaag ggcgtcgacc
     2041 tggacaccgt ggtcggcaag atctcctgga gcggcggccc ggtgcccaac gtggccaaga
     2101 ccccgctggt cggcggccag tggcgcaaga gcgacggcaa gtacccgttc gagctggtga
     2161 tcgtgtccaa caaggaccac cccgacatcc ccaagggcgg gtccctggaa cccatcccct
     2221 acgcctagga cggcgggctt ggccgggcgg gcgctggagc tggacgggct gtcgaagtcg
     2281 ttcggggcgc tcaaggtcat cgacgacctg cacgtgcacc tggacgaggg cgaggccctc
     2341 ggggtggtcg ggcccaacgg ggccggcaag acgaccatgc tccacctggc cgctgggcag
     2401 ctgcggcccg acgccggccg ggtccgcctc gccggccagg acgtcaccgg cctgccggcc
     2461 cgggcccgct gccagctcgg cctggcccgc accttccagg tcccccagcc gttctcaggg
     2521 atgaccgtgt acgagaacgt gctcgtcggg gcgaccttcg gcgcaggggg tcgggggggc
     2581 tcaagcctag ccccccggag agtcgcccat ccggacgcgc accgggccag cctggagg
//
LOCUS       DASSWR010000086         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_597256,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000086 DASSWR010000000
VERSION     DASSWR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597256"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="VFO47_01400"
     CDS             complement(<1..1737)
                     /locus_tag="VFO47_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787387.1"
                     /translation="MQPSQPPDTTAPLAVVNGVLEHITYTNEDTGYTVARVATDRGGD
                     PVTVVGPLLGAQPGEHLHLEGRWRNHQLYGRQFEVRSYRAVLPATVQGIRRYLGSGMI
                     KGIGPKMAERIVDHFGVDTLRVLEEDPGRLRSVPGLGPTRTAMIEQAWEEQKAIKDVM
                     VFLQGVGVSTSLAVRIYKAYRDEAIKVVTHAPYRLATEVWGIGFKTADKIAQGVGIAY
                     DSGERVKAGLQFTLSQASELGHCYLPEPELVARASEILGVRPELAQSCLDELVRQQGV
                     IRDPVPIPGFDDGVDGIYLVPFHRAEVSLAAGLLRLRSAPEQADRLPMFRNVDWAAAF
                     AWLERVTGSRLATEQETAVRLALTERVAVLTGGPGCGKSFTVRAVVALAQAKKARVVL
                     AAPTGRAAKRLSELAGMDAQTLHRLLELRPGGDAAFNQDRPLEADLVVVDEASMLDLL
                     LANKLVKAVPPGAHLLLVGDVDQLPSVGPGEVLRDLLGAGKGAGVLPRVRLTQVFRQA
                     QQSGVVTNAHRINAGHPPLTSGLGDFFLVPEDDPELVAELTVDIVVNRLPRRFGLDPR
                     RDVQVLCPMYRGP"
     gene            complement(1775..2257)
                     /gene="ssb"
                     /locus_tag="VFO47_01405"
     CDS             complement(1775..2257)
                     /gene="ssb"
                     /locus_tag="VFO47_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET8787388.1"
                     /translation="MSDNQVTLSGNLTDDPELRFTPNGVAVANFRLAVDQRVWDTDGW
                     KDGESSYFRVSVWRDQAEHVSRSLHKGARCVVVGRLRSRSWETPEGQRRSAVEVDAEE
                     VGMSLRFGAAASAPAPVTPAPPPAEAATERPAPAKAGGNGSRRGRQPAKAAARDEAPF
                     "
     gene            complement(2455..2706)
                     /locus_tag="VFO47_01410"
     CDS             complement(2455..2706)
                     /locus_tag="VFO47_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787389.1"
                     /translation="MASSRQQAAQEAHQRSERLAAVAQDLADQASAAATAAEAAHQAA
                     DAADRDVDEAADRVRAAQEALEAAEAAHAAAVTALEDQA"
BASE COUNT          342 a          962 c         1029 g          388 t
ORIGIN      
        1 cgggccgcgg tacatggggc agagcacctg cacgtcgcgc cgcgggtcca ggccgaagcg
       61 gcgtggcagg cggttgacca cgatgtcgac ggtcagctcg gccaccagct cggggtcgtc
      121 ctcggggacg aggaagaagt cacccaggcc gctggtcagc ggcgggtggc cggcgttgat
      181 gcggtgggcg ttggtgacca cgccgctctg ctgggcctgg cggaagacct gggtcaggcg
      241 cacccggggg agcaccccgg ctcccttgcc ggcgccgagc aggtcccgca ggacctcgcc
      301 cgggccgacg cttgggagct ggtccacgtc gccgaccagc agcaggtggg cgccgggcgg
      361 gaccgccttg acgagcttgt tggccagcag caggtccagc atggaggcct cgtcgaccac
      421 caccaggtcg gcctccagcg ggcggtcctg gttgaaggcg gcgtcgccgc ccgggcgcag
      481 ctccaataat cggtgcaggg tctgggcgtc catcccggcc agctcggaca ggcgcttggc
      541 ggcgcggccg gtgggggcgg cgagcaccac ccgggccttc ttggcctggg cgagggcgac
      601 gacggcgcgg acggtgaagc tcttgccgca gccggggccg ccggtgagca cggccacccg
      661 ctcggtcagg gcgagccgga ccgccgtctc ctgctcggtg gcgaggcggc tcccggtgac
      721 ccgctccagc cacgcgaagg cggccgccca gtcgacgttg cggaacatgg gcagccggtc
      781 ggcctgctcg ggcgccgagc gcagccgcag cagccccgcg gccagcgaga cctcggcccg
      841 gtggaagggg accaggtaga tgccgtccac gccgtcgtcg aagccgggga tcggcaccgg
      901 gtcgcggatc acgccctgct ggcgcaccag ctcgtccagg caggactggg ccagctccgg
      961 ccggacgccg aggatctccg aggccctggc caccagctcc ggctcgggca ggtagcagtg
     1021 gccgagctcg ctggcctggg agagggtgaa ctggaggccg gccttgacgc gctcgccgct
     1081 gtcgtaggcg atgccgacgc cctgggcgat cttgtcggcg gtcttgaagc cgatgcccca
     1141 cacctcggtg gccaggcggt agggcgcgtg cgtgacgacc ttgatggcct catcgcggta
     1201 ggccttgtag atccgcacgg ccagcgaggt cgagaccccg acgccctgga ggaagaccat
     1261 gacgtccttg atggccttct gctcctccca ggcctgctcg atcatggccg tccgcgtggg
     1321 gccgagaccg ggcacgctgc gcagccgccc ggggtcctcc tcgagcaccc gcagggtgtc
     1381 gaccccgaag tggtcgacga tgcgctcggc catcttgggg ccgatcccct tgatcatccc
     1441 cgagcccagg tagcggcgga tgccctggac ggtggcgggg agcacggccc ggtagctgcg
     1501 tacctcgaac tggcgcccgt acagctggtg gttgcgccag cggccctcca ggtggagatg
     1561 ctccccgggc tgggccccga gcagcgggcc gaccacggtg accgggtcac caccgcgatc
     1621 ggtggcgacc cgggcgacgg tgtagccggt gtcctcgttg gtgtaggtga tgtgttccag
     1681 cacaccgttg accactgcca gaggagcggt cgtgtccggc ggctgggaag gctgcatggt
     1741 gagggtcaag ggactccccc cgaggtcaga caggtcagaa cggcgcctcg tcacgggcgg
     1801 ccgccttggc gggctggcgg ccccggcgcg acccgttgcc gccggccttg gccggcgcgg
     1861 gacgctcggt ggcggcttcg gcgggcggcg gggccggggt gaccggcgcc ggggcggagg
     1921 cggccgcccc gaaccgcagg ctcatgccca cctcctcggc gtcgacctcg acggccgagc
     1981 gccgctggcc ctccggggtc tcccaggagc ggctcctgag ccggcccacg accacgcacc
     2041 gggccccctt gtggaggctg cggctgacgt gctcggcctg gtcgcgccag acactcacgc
     2101 ggaagtagga cgactcaccg tccttccagc cgtcggtatc ccagacccgc tggtcgacgg
     2161 cgagccggaa gttggccacc gccaccccgt tgggggtgaa gcgcagctcc gggtcgtcgg
     2221 tcaggttgcc gctgagggtc acctggttgt cgctcacctt cgagctcctt ccgttgttct
     2281 gcgtaggacg atcctacgcg ctcatacgaa catacgttcg aaatgtgaca gtggctgcgc
     2341 cgggaacgga ctaccccggc acttggtaca gattggtatg agtcggtcag aaccctggcg
     2401 cccttggcgg ccgccccagc catcaccctc ggccgtccgc tgcggtcgcc gccgctaggc
     2461 ctggtcctcc agggcggtca ccgcggcggc atgggcggcc tcggccgcct ccagcgcctc
     2521 ctgggcggcc cgtacccggt cggccgcctc gtccacgtcg cggtcggcgg cgtcggccgc
     2581 ctggtgggcc gcttcggcgg cggtggcggc cgccgaagcc tggtcggcga ggtcctgggc
     2641 gacagccgcc agccgctcgg agcgctggtg ggcctcctgg gcggcctgct ggcggctgct
     2701 ggccagcccc tgggcctgat c
//
LOCUS       DASSWR010000087         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_568909,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000087 DASSWR010000000
VERSION     DASSWR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568909"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1175
                     /locus_tag="VFO47_01415"
     CDS             261..1175
                     /locus_tag="VFO47_01415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET8787390.1"
                     /translation="MSVLDELEQAVRQVAERVGPSVVGIGRGPGRGSGVVLADGLVAT
                     NAHNLRGEETTVSFGGDRSAVGRVAGVDVDADLAVIAVDTGGAPAIAWADDAGARVGA
                     AVFALANPGGQLRVTFGLVSAVDRAFRGPRGRRIAGSIEHTAPLPRGASGGPVVDAGG
                     RLLGIDTNRLGDGFYLALPADAELRQRLDALGRGESTARPRLGVGVAPARVARQLRRS
                     VGLPERDGLLVQVVEDGGPADQAGLRTGDLLVEAGGRPLTDADELHEALDQVGEDQAL
                     TLRVVRGTDELTVTVRFGGSTAEQVGSA"
     gene            complement(1172..1558)
                     /locus_tag="VFO47_01420"
     CDS             complement(1172..1558)
                     /locus_tag="VFO47_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8787391.1"
                     /translation="MDEQEVRELFFERLEHLSPEAEYELRHPDYVMEMPQSGERIRGR
                     ENMRAFQEAYPNPPTITPRRVAGSGDVWVIEGRSDYGGGQVFNVAMIVEFRDGKIWKD
                     TRYYAEPFEPPAWRSQWVEPMGETGT"
     gene            complement(1584..3215)
                     /locus_tag="VFO47_01425"
     CDS             complement(1584..3215)
                     /locus_tag="VFO47_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8787392.1"
                     /translation="MADYDAIVCGGGSNGLAAAIRLAEAGWSVCLLEANDDLGGAART
                     LESTLPGFKHDFGAAFFPLVAPSPSIVGSDLAPYGVRFVHAQLPAAHPFPGNQAIALG
                     RSVGETAASIDKVHLGDGAAWFALDEAFGEVMKHFLRAQMQRWPFEELLRVVGRLRLS
                     GGLQFARLVVSGVSAVVDRFESEQAKAFLTGPAMHADLQPEEPGTGIYALLLSMLGQR
                     FGMPVVEGGTGMITAGLAAKARHDGVEIATGGRVDRIVVERGRAVAVEAGGDTVSARR
                     AVLCALDVGALVRLAGPESFPPRALAEVRHYRPGLGTFKVDWALDGQVPWAAEECRRA
                     GVVHVGDTLTEMSRSAWEAAHGLLPGKPTLILGQQSLADPSRAPAGKHTLWGYAHVPS
                     VPTADAARPRAKAGWDRSAEPFLERMEAAIEAHAPGFRDRVLARRAWSPADLEAADAS
                     LAGGDISAGSFAIDQQLLFRPGPSWWRWGTPVKGLYLAGASVPPGAGVHGACGDLAAR
                     QAIADHDRPRQLALTAAGAGAVATAFLAAVRARRR"
BASE COUNT          436 a         1219 c         1312 g          413 t
ORIGIN      
        1 ggactcggcc agctccgcat accgggcgat ccggtcgaag gtggccggct gctggtcggg
       61 gaacgagatg tcgtagtggg gaagggcgat tcccagcttc atggaaggca gtcttccggt
      121 gtcgccacca ccccgtcaac gcggggccgt tggctggcga tagagacggg gccccgtcgg
      181 ccttggcgtc cccttgcgga tccctcgctt cgcgactagc ttacatgatt acaacgttgc
      241 ggagcaggaa ggagcggcgc atgagcgtgc tcgacgagct ggagcaggcc gtccggcagg
      301 tggcggagcg ggtcgggccc tcggtggtcg ggatcggccg ggggccgggg cgcgggtccg
      361 gggtggtgct ggccgacggg ctggtggcca ccaacgccca caacctccgc ggcgaggaga
      421 cgacggtctc cttcggcggc gaccgcagcg ccgtcgggcg ggtggccggg gtcgacgtcg
      481 acgccgacct ggccgtgatc gcggtcgaca ccggcggggc gccggccatc gcctgggccg
      541 acgacgccgg ggcgagggtc ggggcggcgg tgttcgccct ggccaacccg ggcgggcagc
      601 tgcgggtgac cttcgggctg gtctcggcgg tggaccgggc cttccgggga ccgcgggggc
      661 ggcggatcgc cggcagcatc gagcacaccg cgcccctgcc ccggggcgcc tcgggcgggc
      721 cggtggtcga cgccggcggc cggctgctcg gcatcgacac caaccggctg ggcgacggct
      781 tctacctggc cctgccggcc gacgccgagc tgcgccagcg cctggacgcc ctcggccggg
      841 gcgagtccac ggcccggccc cggctcggcg tcggcgtcgc cccggcaagg gtggccaggc
      901 aactgcgccg gtcggtgggc ctgcccgagc gggacggcct gctggtccag gtggtcgagg
      961 acggcggacc ggccgaccag gccggcctgc gcaccggcga cctcctggtc gaggccggcg
     1021 gccggccgct caccgacgcc gacgagctgc acgaggcgct cgaccaggtc ggcgaggacc
     1081 aggccctcac cctccgcgtg gtccggggca ccgacgagct gacggtgacg gtcaggttcg
     1141 gcggcagcac cgccgagcag gtgggcagcg cctaggtccc cgtctccccc atcggctcga
     1201 cccattggga gcgccaggcc ggcggctcga acggctcggc gtagtagcgg gtgtccttcc
     1261 agatcttgcc gtcgcggaac tccacgatca tggccacgtt gaagacctgc ccgccgccgt
     1321 agtcggaccg gccctcgatc acccagacgt ccccggaccc ggccacccgg cgcggggtga
     1381 tggtcggcgg gttgggatag gcctcctgga acgcccgcat gttctcgcgg ccgcggatgc
     1441 gctcccccga ctggggcatc tccatcacgt agtcgggatg acggagctcg tactcggcct
     1501 cgggactcag atgctcgagc cgctcgaaga acagctcgcg gacctcctgc tcgtccaact
     1561 gccctcctct gggtgagccg gtgtcagcgg cggcgggcgc ggaccgcggc caggaacgcc
     1621 gtggccaccg cgccggcgcc ggcggcggtg agggcgagct ggcgggggcg gtcgtggtcg
     1681 gcgatggcct ggcgggcggc caggtcgccg caggcgccgt ggaccccggc gccggggggg
     1741 acgctggcgc cggccaggta caggcccttg accggggtgc cccagcgcca ccaggacgga
     1801 ccggggcgga acaggagctg ctggtcgatc gcgaacgagc cggcggagat gtcgcccccg
     1861 gccaggctgg cgtcggccgc ctccaggtcg gccgggctcc aggccctgcg ggccaggacc
     1921 cggtcgcgga acccgggcgc gtgggcctcg atggccgcct ccatccgctc caggaacggc
     1981 tcggccgacc ggtcccagcc ggccttggcc cggggccggg ccgcgtccgc ggtcgggacc
     2041 gacgggacgt gggcgtagcc ccacagggtg tgcttgccgg ccggggcccg tgaggggtcg
     2101 gccagcgact gttggccgag gatgagcgtc ggcttgcccg gcagcaggcc gtgcgcggcc
     2161 tcccaggcgg aacggctcat ctcggtcagg gtgtcgccga cgtggacgac gccggcccgg
     2221 cggcactcct cggccgccca cggcacctgg ccgtccaggg cccagtcgac cttgaaggtc
     2281 ccgagaccgg gccggtagtg gcggacctcg gccagggccc ggggcgggaa cgactccggc
     2341 ccggccagcc ggaccagcgc gccgacgtcc agggcgcaga gcaccgcccg gcgggcgctg
     2401 accgtgtcgc cgccggcctc gacggccacc gcccggcccc tctcgaccac gatccggtcg
     2461 acccggccgc cggtggcgat ctcgaccccg tcgtgacgcg ccttggcggc caggccggcg
     2521 gtgatcatcc cggtgccgcc ctcgaccacc ggcatgccga agcgctggcc gagcatggac
     2581 agcagcaggg cgtagatgcc ggtgcccggc tcctcgggct ggaggtcggc gtgcatggcc
     2641 gggccggtca ggaacgcctt ggcctgctcg gactcgaagc ggtcgacgac cgcgctgacc
     2701 cccgagacga ccaggcgggc gaactggagg ccgcccgaca ggcgcagccg ccccaccacc
     2761 ctgagcagct cctcgaacgg ccagcgctgc atctgggccc ggaggaagtg cttcatgacc
     2821 tcgccgaacg cctcgtccag ggcgaaccag gccgccccgt cgcccaggtg gaccttgtcg
     2881 atcgaggcgg cggtctcgcc caccgagcgg cccagggcga tcgcctggtt gccggggaac
     2941 gggtgggcgg ccggcagctg ggcgtggacg aagcgcacgc cgtagggggc aaggtcgctc
     3001 ccgacgatcg agggcgacgg cgccaccagc gggaagaagg cggccccgaa gtcgtgcttg
     3061 aaccccggca gggtgctctc cagggtcctg gccgcccccc cgaggtcgtc gttggcctcc
     3121 agcaggcaca ccgaccagcc cgcctcggcc aggcggatgg cggcggcgag cccgttggag
     3181 ccgccgccgc agacgatcgc gtcgtagtcg gccatcaccc cgagcctact ctgctcgcga
     3241 cggcgaggcg tcccggcggt cgctacactc caggaagaag ggcggtttcg cccggcacgg
     3301 ctcagaaggg accggttctg atggtcacgc atgatgaggt catgacagcg ctcgggcagg
     3361 tgatggaccc ggagatccac
//
LOCUS       DASSWR010000088         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1246838, whole genome shotgun sequence.
ACCESSION   DASSWR010000088 DASSWR010000000
VERSION     DASSWR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246838"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFO47_01430"
     CDS             <1..461
                     /locus_tag="VFO47_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004059929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET8787393.1"
                     /translation="GFMPGTSQEWGGIIRHGAKLLYAYAEATVPKLTVITRKAYGGAY
                     DVMASKPLRADINFAWPTAEIAVMGPQGAVNVIFRKEIERADDPVARRDELVAEYTER
                     FANPYIAAERGYIDEVIEPQVTRPRLIQALEMCVTKREVRPPRKHGNIPL"
     gene            458..766
                     /locus_tag="VFO47_01435"
     CDS             458..766
                     /locus_tag="VFO47_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787394.1"
                     /translation="MRSSESAFGRQPTDSARQGRAGPGDILVLHTPDGLRFQLVKGVP
                     EGDEAEVLAAAIDRVEAWDKSQELGPWVTNTRPGIATRAYPAGTRWGASLRSTWGRDL
                     "
     gene            complement(789..1100)
                     /locus_tag="VFO47_01440"
     CDS             complement(789..1100)
                     /locus_tag="VFO47_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET8787395.1"
                     /translation="MEAACRQVDTTLFYSPEGERGPRKERREAAAKQICGSCKVMELC
                     AAYAIATREPYGTWGGLSENDRRELVRRIDPRMAQLRYRTALAAWEETTAWSRPPADG
                     N"
     gene            1347..>2904
                     /locus_tag="VFO47_01445"
     CDS             1347..>2904
                     /locus_tag="VFO47_01445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET8787396.1"
                     /translation="MEKVLVANRGEIAVRVLRACRELGLVAVAVYSDADRDALHVEVA
                     DEAWHIGETAPAKSYLNVDGLVEVARRARIDAVHPGYGFLAENAAFAQAVLDAGLRWV
                     GPKPAAIAAMGDKVSARRVAEQAKAPLVPGTVEPLAGPEAAVAFGDRHGYPLALKAAF
                     GGGGRGMKVVRTAGDVAAGLESARRESKAAFGRDEIYVERYLDAPRHVEAQILADGRG
                     EVVFLGERDCSLQRRHQKLVEEAPAPGLPGEVRMALGKAACDIARAADYENAGTIEFL
                     YEPATERFYFLEMNTRLQVEHPVTELTAGIDLVHAQLRVAAGEGIPAAYEDVQARGHA
                     IEVRINAEDPGARFMPAPGPITGWREPSGPGVRVDAGVRAGFTVPQAYDSLLAKLIVW
                     GEDRDQARRRMLRALDEFQIEGVPTTIPFHRLAMLDPAFAAGDVSTVLVEEGMDLSSL
                     EPATIEGGGPPTAKPPPRRLVIELEGKRFDVDLFPQEPVKVPERTRTPRSPKALERAR
                     AESGGPGKEVV"
BASE COUNT          388 a         1077 c         1067 g          372 t
ORIGIN      
        1 ccgggttcat gcccggcacc agccaggaat ggggcggcat catccgccac ggggccaagc
       61 tgctgtacgc ctacgccgag gccaccgtgc ccaagctgac cgtgatcacc cgcaaggcct
      121 acggcggggc ctatgacgtg atggcgtcca agccgctgcg ggccgacatc aacttcgcct
      181 ggccgacggc cgagatcgcc gtcatggggc cgcagggcgc ggtcaacgtc atcttccgca
      241 aggagatcga gcgggccgac gacccggtgg cccgccgcga cgagctggtc gccgagtaca
      301 ccgagcgctt cgccaacccc tacatcgccg ccgagcgggg ctacatcgac gaggtgatcg
      361 agccccaggt gacccgcccg cggctcatcc aggccctgga gatgtgcgtg accaagcgcg
      421 aggtccgccc gccccgcaag cacgggaaca tcccgctatg aggagctcgg agtcggcctt
      481 cggccgacag ccgacagact ccgcccggca gggccgggcg gggccgggcg acatcctggt
      541 gctgcacacc cccgacgggc tgcggttcca gctcgtcaag ggggtgccgg agggggacga
      601 ggccgaggtg ctggcggccg ccatcgaccg ggtcgaggcc tgggacaaga gccaggagct
      661 cggcccctgg gtcaccaaca cccgcccggg catcgcgacc agggcctatc cggccggtac
      721 ccgctggggg gccagcctcc gctcgacctg gggccgcgac ctctagccga ccggcgtgcc
      781 ggtcgaagtc agttgccgtc ggccggaggc cggctccacg cggtcgtctc ttcccaggcg
      841 gccagcgccg tgcggtagcg gagctgggcc atgcgggggt cgatccggcg gacgagctcg
      901 cggcggtcgt tctccgacag gccgccccag gtgccgtacg gctcccgggt ggcgatggcg
      961 taggcggcgc acagctccat gaccttgcac gacccgcaga tctgcttggc cgcggcctcg
     1021 cggcgctcct tgcgtggccc ccgctccccc tcgggcgagt agaacagggt ggtgtccacc
     1081 tgccggcagg cggcttccat gtgccaggtc cagaactcct cgagggtgga cggccaggcc
     1141 tggatggggg cttcgctcac cttggctccg cttcttcccg tacgtgtcct ctccctgaac
     1201 cttcgccccc cgccaggttg cggttgcacc acatcgcgtt ccgcaaacac tgtgcgcccg
     1261 ggtccggttg cctgcccagg tcgcggctgg gctacgctgt ccgcgacgcc cggtcgggcg
     1321 tccacccctt gcgaggaggc agccgcgtgg agaaggtgct ggtcgccaac cggggcgaga
     1381 tcgccgtgcg cgtgctgcgc gcctgccgcg agctcggcct ggtggcggtg gcggtctact
     1441 ccgacgccga ccgcgacgcc ctccacgtcg aggtggccga cgaggcctgg cacatcggcg
     1501 agaccgcccc ggccaagagc tacctgaacg tcgacgggct ggtcgaggtg gcccgccggg
     1561 ccaggatcga cgccgtccac cccggctacg ggttcctggc cgagaacgcc gccttcgccc
     1621 aggcggtgct ggacgccggg ctgcgctggg tcgggcccaa gccggccgcg atcgccgcca
     1681 tgggcgacaa ggtctcggcc cgccgggtcg ccgagcaggc caaggcgccg ctggtcccgg
     1741 gcacggtgga gccgctggcc ggcccggagg cggcggtggc cttcggcgac cgccacggct
     1801 acccgctggc cctcaaggcg gccttcggcg gcggcggccg gggcatgaag gtggtccgca
     1861 cggccggcga cgtggccgcc gggctggagt cggcccggcg cgagtccaag gccgccttcg
     1921 gccgcgacga gatctacgtc gagcgctacc tggacgcccc ccgccacgtc gaggcccaga
     1981 tcctggccga cgggcgcggc gaggtggtgt tcctgggcga gcgcgactgc tcgctccagc
     2041 gccgccacca gaagctggtc gaggaggccc cggccccggg cctgcccggg gaggtgcgga
     2101 tggcgctggg caaggcggcc tgcgacatcg cccgggcggc cgactacgag aacgccggca
     2161 ccatcgagtt cctctacgag ccggccacgg agcggttcta cttcctggag atgaacaccc
     2221 ggctccaggt cgagcacccg gtcaccgagc tgaccgccgg catcgacctg gtccacgccc
     2281 agctccgggt ggccgccggc gagggcatcc cggccgccta cgaggacgtc caggcccgcg
     2341 ggcacgccat cgaggtgcgg atcaacgccg aggacccggg ggccaggttc atgccggccc
     2401 cggggcccat caccggctgg cgggagccgt ccggtcccgg ggtgcgggtc gacgccggcg
     2461 tgcgggccgg cttcaccgtc ccccaggcct acgactcgct gctggccaag ctgatcgtct
     2521 ggggcgagga ccgcgaccag gcccggcggc ggatgctgcg ggccctggac gagttccaga
     2581 tcgagggcgt gcccaccacc atcccgttcc accgcctggc catgctcgac ccggcgttcg
     2641 cggccgggga cgtctccacc gtgctggtcg aggagggcat ggacctgtcg tcgctggagc
     2701 cggccacgat cgaggggggc gggccgccga cggccaagcc gccgccccgg cgcctggtca
     2761 tcgagctgga gggcaagcgc ttcgacgtcg acctgttccc acaggagccg gtcaaggtgc
     2821 cggagcggac ccgcaccccc cgctccccca aggccctgga gcgggcccgg gccgagtccg
     2881 gcggtcccgg caaggaggtc gtca
//
LOCUS       DASSWR010000089         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_371225,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000089 DASSWR010000000
VERSION     DASSWR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371225"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..>2396)
                     /locus_tag="VFO47_01450"
     CDS             complement(266..>2396)
                     /locus_tag="VFO47_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HET8787397.1"
                     /translation="AWLAWRRQWKLLGRLLLASVVALVLLARVDRLISEAPVPSPAAI
                     PPAAWVTDISLRLGSGNVAVAVAIYMVARPLMTVPYRRLVVWTIALTTLHVILAGSDL
                     PRDLVAAIACGFIAASSVLLALGRPMRPLEPDQVAAALRRTSIDVTKVHPAAVDARGS
                     RPFFGTMAGGDGVFVKVLGREERSSDLLFRFYRWLRFRGLGDEAIASSLKQSIEHEAL
                     VSVLAADDGIRTPRLLGVSEVDADRLALAFVRIEGSSLDGVPVERLDDEILRGIWRLV
                     AELHRAGIAHRDLRLANLFLDDRGQPWLIDFGFGTVAALPQQLAGDVAELLCSMAVAV
                     GPKRTVDAAVAELRPEQIQAALPRLQPDALSTATRKAMKAHKGLDKQLQQTAADAVGV
                     EKVQLQHLERVQPRHVLTLVATAVAAYLLIHQVTEIKDLPAVLARMDWSWAVLALVAS
                     ALTYLGAALNLMGGFVQRLSLGLTTAEQLGGSFINRITPVKVGGMAVNVRYLQKQGLD
                     PATTIGGVGISMLAAGAMHISLTAVFLVWAGSKANVVDLPSSTTILLVLVIAAAIIGL
                     AVMIRPARRLIRTTVVPQVEKAVHELREALRDPARLSLSFVGALLMDLAYIVALFASV
                     RAFGYPVSVAVAGAVYLAGTAVASAAPTPGGVGAVEAALIAGLTATGVPNELAVPAVL
                     IYRVATFWLPVLPGWLAFTLLTRRDVI"
BASE COUNT          381 a          871 c          865 g          279 t
ORIGIN      
        1 cacaaccccg cctggaccgt ccatgccgac ggccggacaa cgctcgaatg agggccacgg
       61 ccaggcccgc gacccgatcc ccctgttcgg cctgcttcca ggctcgctac cgtcagggga
      121 ggtcgaccgc ctcctcgggg acgtggccgc ggcggaggac gcctcccagc aggccggccg
      181 ccggggtgtg ggcccgcacg gccgcgccgt cggcctcgac ctcgaccccg gcgccctggc
      241 catcgcccgc caggcggagg agcggtcaga tcacgtcccg cctcgtcagg agggtgaagg
      301 ccagccagcc cggcagcacc ggcagccaga acgtggccac ccggtagatc agcacggccg
      361 ggacggccag ctcgttgggg acacccgtgg ccgtcaggcc ggcgatcagg gcggcctcga
      421 cggcgccgac cccgcccggc gtcggggcgg cggaggccac ggcggtgccg gcgaggtaga
      481 ccgccccggc gacggcgacg ctgaccggat agccgaaggc ccggaccgac gcgaacagcg
      541 cgacgatgta ggccaggtcc atgaggagcg cgccgacgaa cgacagcgac agccgggccg
      601 ggtctcgcag ggcctcgcgc agctcatgaa ccgccttctc cacctgtggg acgaccgtgg
      661 tgcggatgag ccgccgggcc ggccggatca tcaccgccag gccgatgatc gcggccgcga
      721 tgacgaggac cagcaggatg gtggtgctcg acggcaggtc cacgacgttc gccttggagc
      781 cggcccacac gaggaacacg gcggtcaggg agatgtgcat cgcccctgcg gcgagcatgg
      841 agatccccac cccgccgatc gtggtcgccg gatcgagccc ctgcttctgg aggtagcgga
      901 cgttgaccgc catgccgccg accttcaccg gggtgatgcg gttgatgaag gatcccccga
      961 gctgctcggc cgtggtgagc ccgagcgaca ggcgctggac gaacccaccc atcaggttca
     1021 gcgcggcgcc gagataggtc agggcggagg cgacgagggc caggaccgcc cagctccagt
     1081 ccatccgggc gaggacggcc ggcaggtcct tgatctcggt gacctggtgg atgaggaggt
     1141 aggccgccac ggcggtggcc accagggtca ggacatgccg gggctgcacc cgctccaggt
     1201 gctggagctg gaccttctcg acacccacgg cgtcggccgc ggtctgctgc agctgcttgt
     1261 ccagcccctt gtgcgccttc atggccttcc gggtcgcggt cgagagggcg tccggctgca
     1321 ggcggggcag cgccgcctgg atctgctcag gccgcagctc cgcgaccgcg gcgtcgacgg
     1381 tccgcttggg gccgaccgcc accgccatcg agcacagcag ctcggccacg tcaccggcga
     1441 gctgctgcgg cagcgccgcc acggtgccga acccgaagtc gatcagccag ggctggccac
     1501 ggtcgtcgag gaagaggttg gccaagcgca ggtcgcggtg ggcgatcccg gcccggtgca
     1561 gctcggcgac caggcgccag atcccccgga ggatctcgtc gtcgagccgc tcgaccggca
     1621 cgccgtccag cgagctcccc tcgatgcgga cgaacgccaa ggccagccgg tccgcgtcga
     1681 cctcggagac accgagcagc cggggggtgc ggatgccgtc gtcggccgcc agcaccgaca
     1741 ccagcgcctc gtgctcgatc gactgcttca gggaggacgc gatcgcctcg tcgcccagac
     1801 cgcggaagcg caaccagcgg tagaaacgga acagcaggtc gctgctacgc tcctcgcggc
     1861 cgaggacctt gacgaagacg ccgtctccgc cggccatggt cccgaagaac ggccgcgatc
     1921 cccgggcgtc gaccgcggcc gggtggacct ttgtgacgtc gatgctggtg cggcgcaggg
     1981 ccgccgcaac ctgatccggc tccagtggcc gcatgggccg tcccaacgcc agcagcaccg
     2041 acgaggcggc gatgaacccg caggcgatcg cggccaccag gtcccgtggc aggtcgctgc
     2101 cggccaggat cacgtgcagc gtggtgaggg cgatggtcca gaccaccaac cgccggtagg
     2161 gaaccgtcat caacggccgg gcgaccatgt agatcgccac ggccacggcc acgttccccg
     2221 agccaagcct gagggagatg tcggtcaccc acgccgccgg cgggatggct gccgggctgg
     2281 gcaccggcgc ctcgctgatg agccggtcca ccctggccag caggaccaag gcgaccacgc
     2341 tggccaggag cagccggccc agcagcttcc actggcgacg ccaggccagc caggcc
//
LOCUS       DASSWR010000090         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1218958, whole genome shotgun sequence.
ACCESSION   DASSWR010000090 DASSWR010000000
VERSION     DASSWR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218958"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1025
                     /locus_tag="VFO47_01455"
     CDS             135..1025
                     /locus_tag="VFO47_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET8787398.1"
                     /translation="MDSRIVVADRIANASAVVALESSVLAQGLPSPANLESADAQQAA
                     VAKANAEPAVIGVIAGRVIVGADQQEVQALASGAVRWKVTVRDLPYVVARGLDGATTV
                     SATAFAAEAAGIGVMATGGIGGIHFGGGDVSADLPQLARSRLTVVCSGPKHVADAAAT
                     IEWLETHGVQLVGYRTDRLPGFLARGRLPLEQVADDVTQVVELIRVRQALGTPGALVL
                     CQDPPEAAAMDPEELGELAAEGAAAARDANVFGKARTPFELDWLHRRTGGRSLVANLA
                     LLEANAGLAGEIAAGVAAAG"
     gene            complement(1030..1287)
                     /locus_tag="VFO47_01460"
     CDS             complement(1030..1287)
                     /locus_tag="VFO47_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET8787399.1"
                     /translation="MSATADPGGRNGTGLPPQAAPSTSGRRVDRLSLVVGVLFVVAGV
                     VFLLDALDVWRLRVDYLVPLGLIVLGLVVLASAWPLRARRP"
     gene            complement(1284..1931)
                     /locus_tag="VFO47_01465"
     CDS             complement(1284..1931)
                     /locus_tag="VFO47_01465"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HET8787400.1"
                     /translation="MTTEPIPPAGPAAPVDHRGPATATVLLGALLVLVGIGWLLDSAG
                     IEVPWRALLPAALIAVGLACVAGAFRGRQHALMVVGVALVVVLSVAVAADWDLDVPWT
                     GGVGDRTERPATPAELGEYELGVGNLVVDLSRLQVPAGTTAVEARLGVGELVVELPDG
                     VSVEVVASSGLGEVQVLGQQEGGFASRIDTAAELGGDRRLRVDARVGLGQVRVDR"
BASE COUNT          284 a          860 c          758 g          203 t
ORIGIN      
        1 gccggggccg gcaccgccct ggccgccagg gcggtggcca ccgccggcgc ctggccggcc
       61 ctgcccgccg gcgacccccc ggagtaccct tgaccgctgc accaggccga ccaacacagg
      121 ggaacgcacc gctcatggac agccgcatcg tcgtcgccga ccgcatcgcc aacgccagcg
      181 ccgtcgtcgc cctcgagtcg agcgtgctcg cccagggcct gccgtccccg gcgaatctcg
      241 agtcggccga cgctcagcag gcggccgtgg ccaaggccaa cgccgagccg gccgtcatcg
      301 gggtgatcgc cggccgggtg atcgtcggcg ccgaccaaca ggaggtgcag gccctggcct
      361 cgggtgccgt ccgctggaag gtgaccgtcc gcgacctgcc gtatgtggtc gcgcgcggcc
      421 tggacggggc gaccacggtg tcggcgaccg cgttcgcggc cgaggcggcc gggatcgggg
      481 tcatggccac cggcgggatc ggcggcatcc acttcggcgg gggcgacgtc tcggccgacc
      541 tgccccagct ggcccgctcg cggctcacgg tcgtctgctc cggccccaag cacgtggccg
      601 acgccgccgc caccatcgag tggctcgaga cccacggggt gcagctggtc ggctaccgca
      661 ccgaccggct ccccgggttc ctggcccgcg gccggctgcc gctggagcag gtcgccgacg
      721 acgtcaccca ggtggtcgag ctgatccggg tccggcaggc tctcggcacc ccgggcgccc
      781 tggtgctctg ccaggacccg ccggaggcgg ccgccatgga ccccgaggag ctgggggagc
      841 tggccgccga gggcgccgcc gccgcccgcg acgccaacgt gttcggcaag gcacggacgc
      901 cgttcgagct ggactggctg caccgccgca ccggcgggcg cagcctggtc gccaacctgg
      961 ccctgctgga ggccaacgcc ggcctggccg gggagatcgc cgccggggtg gccgccgccg
     1021 gctgagccgt cagggccggc gggcccgcag cggccaggcg ctggccagca cgaccagccc
     1081 gagcacgatc aggcccagcg ggaccaggta gtccacccgc agccgccaga cgtccagggc
     1141 gtcgagcagg aacacgaccc cggcgaccac gaacagcacc ccgacgacca gcgacagccg
     1201 gtcgacccgg cgacccgaag tgctcggtgc cgcctgcggc ggcaggccgg ttccattgcg
     1261 accgccaggg tcggcggtcg cgctcaccgg tccaccctga cctggccgag cccgacccgg
     1321 gcgtccaccc gcagccggcg gtcgccgccg agctcggcgg cggtgtcgat ccggctcgcg
     1381 aacccgccct cctgctggcc cagcacctgg acctcgccaa ggccggagct ggccacgacc
     1441 tcgaccgaca ccccgtcggg cagctcgacc accagctcgc cgacccccag ccgggcctcc
     1501 acggcggtgg tcccggccgg gacctggagc cggctcaggt cgaccaccag gttgccgacg
     1561 cccagctcgt actcgcccag ctcggccggg gtggcgggcc gctcggtgcg gtcgccgacc
     1621 ccgccggtcc agggcacgtc caggtcccag tcggccgcca ccgccaccga caggacgacc
     1681 accagggcga ccccgacgac catcagggcg tgctggcggc cccggaaggc gccggccacg
     1741 caggccaggc cgaccgcgat cagggccgcg ggcagcagcg cccgccacgg cacctcgatc
     1801 ccggccgagt cgagcagcca gccgatcccg accagcacca gcagcgctcc cagcagcacc
     1861 gtggccgtgg ccggcccccg gtggtcgacc ggcgcagccg gccccgccgg cgggatcggc
     1921 tcggtcgtca tcgtctgagc cccaccagca tgaccgcgac cccgacggcg acgaccgcga
     1981 ccggccagaa cagctcgtcc aggtcggggg cgagccggtc cagcagcagc accaggccga
     2041 gcacgaccag cgacccgccg aggaccagcc ggccggtctc gcgccggggc tcggcggccg
     2101 gaccg
//
LOCUS       DASSWR010000091         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1076338, whole genome shotgun sequence.
ACCESSION   DASSWR010000091 DASSWR010000000
VERSION     DASSWR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076338"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFO47_01470"
     CDS             complement(<1..851)
                     /locus_tag="VFO47_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8787401.1"
                     /translation="MTSYLPADGRYDTMQYRRVGRSGLLLPAISLGLWQNFGGDRPFE
                     TQRAIVRRAFDLGITHFDLANNYGPPYGSAEENFGRILATDLRGYRDELVISTKAGYD
                     MWPGPYGDHGSRKYLLASLDQSLQRMGLPYVDIFYSHRVDPDTPLEETMGALDTAVRS
                     GRALYAGISSYSPERTRQAAEILRGMGTPLLIHQPSYSMLNRWIEDGLLDALESQGVG
                     CIVFSPLAQGLLTDRYLGGVPEGSRASKPGTIAGDQLSEETLAKVRALHQLAGRRGQS
                     LAQMAVA"
     gene            935..1990
                     /locus_tag="VFO47_01475"
     CDS             935..1990
                     /locus_tag="VFO47_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET8787402.1"
                     /translation="MRRLLPVLCLAVLVAGCSDLGVRSQDAATVNGVGISTDRLSEMT
                     KAQLGQQQQAEQQQGQQEGQDIDSATRQALEGLIQFQLVLDGARSEGITIQEADVDAR
                     MEQLKQQVAAQGQNYEELLKTRQISEEVLRTQQRVQLAVDLVAVKLVPYSSDAALRQL
                     LDRRKEDFLEVRVRHVLVKVKATANQVRQQLVADGDWAAVAKRSSIDTQSKDKAGDLG
                     FTAKGATVKPFETAEYALAGQGDCKGKTSGSCESPISQPVKTQFGYHVLQVVGVRLPA
                     LDNELRAKLEPTIKDRRQQAVQQWFDQQVDKAEVVINPRFGRWDAENGKVIERETAPG
                     AATTTTAGLGGPSPTQP"
     gene            1995..>2915
                     /locus_tag="VFO47_01480"
     CDS             1995..>2915
                     /locus_tag="VFO47_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HET8787403.1"
                     /translation="MTRVVLVASSPRLPLLFPPQTWRALDAARPVWLLDDGHPSLPAL
                     EVAEIPWEVLAPADDTGPAGRDLLLVGQGLDLEAVALARRQADALLDHARRGGTATLL
                     LPPVNDGPLVQLVADRAARSHIEVEAVYPLGEPKGAALLDLVATETRLRGPGGCPWDR
                     EQTHASLARHLVEEAYEVLDAIEEGDPEHLREELGDLLLQVVFHAQLAEDAGDFDVDG
                     VARAITEKLVRRHPHVFGDLRVASAGEVVRNWEAIKREEEGRTDPLAGIPSALPALQL
                     TAKLQKRAPAGGLADDAGSAARVRERLEALA"
BASE COUNT          421 a         1058 c         1064 g          372 t
ORIGIN      
        1 caggcgaccg ccatctgggc caggctctgg ccgcgccggc cggcgagctg gtgcagggcc
       61 ctgaccttgg ccagggtctc ctcgctgagc tggtcgccgg cgatggtgcc gggcttgctg
      121 gcccgcgacc cctcgggcac gccgcccagg tagcggtcgg tcagcagacc ctgggccagc
      181 ggcgagaaca cgatgcagcc gaccccctgg gactccagcg cgtccagcag gccgtcctcg
      241 atccagcggt tcagcatcga gtacgacggc tggtggatca gcaggggcgt gcccatcccg
      301 cggaggatct cggccgcctg ccgggtccgc tccggcgagt aggaggagat gccggcgtac
      361 agcgcccggc ccgagcgcac ggccgtgtcc agggccccca tcgtctcctc cagcggcgtg
      421 tccgggtcca cccggtggga gtagaagatg tcgacatagg gcaggcccat gcgctgcagg
      481 ctctggtcga ggctggccag gaggtacttg cgcgagccgt ggtcgccgta gggccccggc
      541 cacatgtcgt agccggcctt ggtcgagatc accagctcgt cgcggtagcc gcgcaggtcg
      601 gtggcgagga tgcggccgaa gttctcctcg gccgagccgt agggcgggcc gtagttgttg
      661 gccaggtcga agtgggtgat gccgaggtcg aaggcgcggc gcacgatcgc ccgctgggtc
      721 tcgaacggcc ggtcgccgcc gaagttctgc cacaggccca gggagatggc gggcagcagg
      781 agcccgctgc gcccgacccg gcggtactgc atggtgtcgt agcggccgtc ggccggcagg
      841 tagctcgtca tcgcggcatt gtcgcacccc tccggcggcg cccgccggta tgcttgtgca
      901 ttgtcgtcgt cgagtcgtga acgtgaggtc gtccgtgcgc cgtctgctcc ccgtcctgtg
      961 tctcgccgtc ctcgtcgccg gctgctcgga ccttggcgtc cgcagccagg acgcggcgac
     1021 ggtcaacggc gtcggcatct cgaccgaccg cctcagtgag atgaccaagg cccagctcgg
     1081 ccagcagcag caggccgaac agcaacaggg ccagcaggag ggccaggaca tcgacagcgc
     1141 gacccggcag gcgctggagg gcctgatcca gttccagctg gtgctggacg gggccaggag
     1201 cgagggcatc accatccagg aggccgacgt cgacgcccgc atggagcagc tcaagcagca
     1261 ggtggcggcc cagggccaga actacgagga gctgctcaag acccggcaga tctccgagga
     1321 ggtcctccgc acccagcagc gggtccagct ggcggtcgac ctggtcgcgg tcaagctcgt
     1381 cccctactcc tccgacgccg cgttgcgcca gctgctcgac cggcgcaagg aggacttcct
     1441 ggaggtccgc gtccgccacg tgctggtcaa ggtcaaggcg accgccaacc aggtccgcca
     1501 gcaactggtc gccgacggcg actgggccgc ggtggccaag cgctcctcga tcgacaccca
     1561 gagcaaggac aaggccggcg acctcggctt caccgccaag ggggcgaccg tgaagccgtt
     1621 cgagacggcc gagtacgccc tcgccggcca gggcgactgc aagggcaaga ccagcggcag
     1681 ctgcgagtcg ccgatctccc agccggtcaa gacccagttc ggctaccacg tcctccaggt
     1741 cgtcggggtg cggctgccgg cgctcgacaa cgagctgcgg gccaagctcg agccgaccat
     1801 caaggaccgc cgccagcagg ccgtccagca gtggttcgac cagcaggtcg acaaggccga
     1861 ggtcgtcatc aacccccgct tcggccgctg ggacgccgag aacggcaagg tgatcgagcg
     1921 cgagaccgcc cccggcgcgg ccacgaccac cacggccggc ctcggcgggc cgtccccgac
     1981 ccagccctag gcgtatgacc agggtggtgc tggtcgcgtc cagcccgcgg ctcccgctgc
     2041 tgttcccgcc ccagacctgg cgggcccttg acgccgcccg gccggtgtgg ctgctcgacg
     2101 acgggcaccc gtcgctgccg gcgctcgagg tcgccgagat cccctgggag gtgctggccc
     2161 cggccgacga caccggcccc gccgggcgcg acctgctcct ggtcggccag ggcctcgacc
     2221 tggaggcggt ggccctggcc cgtcggcagg ccgacgcgct gctcgaccac gcccgccgcg
     2281 gcgggacggc cacgctgctg ctgccgccgg tcaacgacgg gccgctggtg cagctggtcg
     2341 ccgaccgggc cgcccgcagc cacatcgagg tcgaggccgt ctacccgctg ggcgagccca
     2401 agggggcggc cctgctcgac ctggtggcga ccgagacgcg gctgcgcggg cccggcggct
     2461 gcccctggga ccgcgagcag acccacgcct cgctggccag gcacctggtc gaggaggcct
     2521 acgaggtgct cgacgcgatc gaggagggcg accccgagca cctgcgcgag gagctcggcg
     2581 acctgctcct ccaggtggtc ttccacgccc agctggccga ggacgccggc gacttcgacg
     2641 tcgacggggt ggccagggcg atcaccgaga agctggtccg gcgccacccg cacgtcttcg
     2701 gcgacctgcg ggtcgcctcg gccggcgagg tcgtccgcaa ctgggaggcg atcaagcgtg
     2761 aggaggaggg ccgcaccgac ccgctggccg gcatcccctc cgccctgccg gcgctccagc
     2821 tcacggccaa gctccagaag cgggccccgg ccggcggcct ggccgatgac gccgggtcgg
     2881 cggccagggt gcgggaacgg ctggaggcgc tggcc
//
LOCUS       DASSWR010000092         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_342737,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000092 DASSWR010000000
VERSION     DASSWR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342737"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFO47_01485"
     CDS             <1..486
                     /locus_tag="VFO47_01485"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HET8787404.1"
                     /translation="GVVYWFLSYEWLGTVCLLLLGAGPGFAGLILIQEQRQRGGTGEN
                     RADAMRRLAGIPPQDPPGPKDLAAEDLGVLPLPTIWPLAAALGFTVLFTGLIYGLWLV
                     ILGLGLLLYATWGWLAAVNRENRYGRVLADTEPPSPQSGPVSDPRPHPDPEAQERRDR
                     P"
     gene            complement(499..1923)
                     /locus_tag="VFO47_01490"
     CDS             complement(499..1923)
                     /locus_tag="VFO47_01490"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HET8787405.1"
                     /translation="MTERSFKYLDERLGTASLARTALRKVFPDHWSFLLGEIALFSLV
                     VLIFTGVFLTFFYTPSVQEVIYDGPYEPLRGAHVTAAYDSVMRISFEVRAGLLMRQTH
                     HWAALVFVAAIVIHLCRVFFTGAFRRPREVNWLVGILLLFLALGEGFTGYSLPDDLLS
                     GIGLRIFYAVTLAIPVIGTWATYLLFGGEFPAPDIISRLFVFHVMLLPALLLALVGVH
                     MAILVLQKHTQFPGHGRTERNVVGKRLWPEQTFKSLSLLLFTTAVLALLGGLAQINPV
                     WQYGPFEPFNVTSPAQPDWYIGFLDGALRLGPPWDLTLFGFTISEVFWPAVLFPGIAF
                     GILTLWPWIERRFTKDRAEHHLLDRPRDTPVRTGIGAAGLMVFFIMFLEGGNDIFGVL
                     LNVPVETITRILQWGLVVAPIVTFLVTYWICKGLSRSRLRPAQINAGVRLSRTADGGY
                     ETVSLEPASSDGERAPAATGPHGE"
     gene            complement(1932..2870)
                     /locus_tag="VFO47_01495"
     CDS             complement(1932..2870)
                     /locus_tag="VFO47_01495"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8787406.1"
                     /translation="MTDSDQQGSERVDVDRQTEQGPANRELTGSQRRAERRTAIALAV
                     TVVAGIGLFVVYFSGGQTQVEGVLFAVALGGLGVALGIWANHLLDAREVVEERHELSS
                     GAAGREAFGDALSEEVGPVLGRRSMLLKLLAGAGGALGLALLLPVLSLGPAPGNTLKM
                     TPWRRGSRVVDEEGEPVTLGQVPEDGFLTVFPEGHEGAADAQTLLIHVRPGSLKLPPE
                     KMAGVPEGTHVAYSKICTHAGCPVGLYRAESQSLICPCHQSQFDVSDGAKPFFGPAAR
                     PLPQLPLGVDEDGILVAQGDFPEPVGPAFWDMNRGR"
     gene            complement(2899..>3666)
                     /locus_tag="VFO47_01500"
     CDS             complement(2899..>3666)
                     /locus_tag="VFO47_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8787407.1"
                     /translation="ALLLTPTTGTASSSRRAAAAAAQQQPSDSPALNARGRELYENAC
                     ASCHGPDPAGPSYYPTVPSLNDVGGAAAVNWVLRTGRMPWKDNKGPAIERGEQRFSDE
                     DVHALVVYIGKAVGDAQLPDVDPARGDLQRGRELYGQACAACHGMNGAGAALGGENIA
                     VSLQDVHPQDVAEAIKIGPGQMPVGGGMTDYEFGTAKSQQDVDDIAAYVESLRTDPYN
                     EGGAPIGGKGPVPEGFVAWVIGLGLLVVASRWIAGRG"
BASE COUNT          521 a         1280 c         1382 g          483 t
ORIGIN      
        1 ggggtcgtgt actggttcct cagctacgaa tggctgggca cggtgtgcct gctgctgctg
       61 ggcgccggcc ccggcttcgc cgggctgatc ctgatccagg agcagcgcca gcggggcggc
      121 accggcgaga accgcgccga cgccatgcgc cgcctggccg gcatcccgcc ccaggacccc
      181 cccggcccca aggacctggc cgccgaggat ctgggcgtgc tgcccctgcc caccatctgg
      241 cccctggccg ccgccctcgg cttcaccgtc ctgttcaccg gcctcatcta cggcctctgg
      301 ctggtcatcc tcggcctggg cctgctcctc tacgccacct ggggctggct ggccgccgtc
      361 aaccgcgaga accgctacgg ccgcgtcctg gccgacaccg agcccccctc accccagtcc
      421 ggcccggtgt cggacccgag gccgcacccc gacccggagg cgcaggagcg tcgcgaccgc
      481 ccctgagcgg cccggccgct actcgccgtg gggcccggtg gccgccgggg cccgctcgcc
      541 gtcgctggag gcgggctcga ggctgaccgt ctcgtagccg ccgtcggcgg tgcggctgag
      601 gcggacgccg gcgttgatct gggccgggcg gaggcggctg cggctgaggc ccttgcagat
      661 ccagtaggtg accaggaagg tcacgatcgg ggcgaccacc aggccccact ggaggatgcg
      721 ggtgatggtc tcgaccggca cgttgagcag gaccccgaag atgtcgttgc cgccctccag
      781 gaacatgatg aagaacacca tcaggccggc ggcgccgatg ccggtgcgga ccggggtgtc
      841 gcgggggcgg tcgaggaggt ggtgctcggc ccggtccttg gtgaagcggc gctcgatcca
      901 gggccacagg gtgaggatgc cgaaggcgat gccggggaac agcacggccg gccagaacac
      961 ctcggagatg gtgaacccga acagggtcag gtcccagggc gggccgaggc gcagggcgcc
     1021 gtcgaggaag ccgatgtacc agtcgggctg ggccggcgag gtcacgttga agggctcgaa
     1081 cggcccgtac tgccagacag ggttgatctg ggccaggccg cccagcaggg cgagcacggc
     1141 ggtggtgaac agcagcagcg acagcgactt gaaggtctgc tcgggccaca gccgcttgcc
     1201 gaccacgttg cgctcggtgc ggccgtggcc ggggaactgg gtgtgcttct gcagcaccag
     1261 gatggccatg tggaccccga ccagggcgag cagcagggcc gggagcagca tcacgtggaa
     1321 cacgaacagc cgcgagatga tgtcgggggc cgggaactcg ccgccgaaga ggagataggt
     1381 cgcccaggtg ccgatcaccg ggatggccag ggtcacggcg tagaagatgc gcaggccgat
     1441 gccgctgagc aggtcgtcgg ggagcgagta gccggtgaag ccctcgccaa gggccaggaa
     1501 cagcagcagg atgccgacca gccagttgac ctcgcggggg cggcggaagg cgccggtgaa
     1561 gaacacccgg cacaggtgga tcacgatcgc ggccacgaac accagggccg cccagtggtg
     1621 ggtctggcgc atcagcaggc cggccctgac ctcgaaggag atccgcatga ccgagtcgta
     1681 ggcggccgtc acgtgcgcgc cccgcagggg ctcatacggg ccgtcgtaga tgacctcctg
     1741 gaccgacggg gtgtagaaga aggtcaggaa gacgccggtg aagatcagca ccacgaggct
     1801 gaacagggcg atctcgccga gcaggaacga ccagtggtcg gggaagacct tgcgcagggc
     1861 cgtgcgggcc aggctggccg tgcccagccg ctcgtccagg tacttgaagg accgctcggt
     1921 caccgtgctc atcatcggcc tcgattcatg tcccagaacg ccggcccgac gggttcgggg
     1981 aagtccccct gggcaaccag gatgccatcc tcgtcgaccc caaggggcag ctggggcagg
     2041 ggccgggcgg ccggcccgaa gaacggcttg gccccgtcgg acacgtcgaa ctgcgactgg
     2101 tggcaggggc agatgagcga ctggctctcg gcccggtaca gccccaccgg gcagccggcg
     2161 tgggtgcaga tcttggagta ggcgacgtgg gtgccctcgg gcaccccggc catcttctcc
     2221 gggggcagct tcagcgaccc cgggcggacg tggatgagca gggtctgggc gtcggcggcg
     2281 ccctcgtggc cctcggggaa cacggtcagg aagccgtcct cggggacctg gccgagggtg
     2341 accggctcgc cctcctcgtc gaccacccgc gagccccgcc gccacggggt catcttgagg
     2401 gtgttgccgg gggccgggcc gagcgacagc accggcagca gcagggccag cccgagcgcc
     2461 ccgccggccc cggcgagcag cttgagcagc atcgagcggc ggccgaggac ggggccgacc
     2521 tcctcggaga gggcgtcccc gaacgcctcc cggccggcgg cgccgctgct cagctcgtgg
     2581 cgctcctcga ccacctcgcg ggcgtcgagc aggtggttgg cccagatgcc gagggcgacc
     2641 ccgagcccgc ccagggcgac ggcgaacagg accccctcga cctgggtctg gccgccgctg
     2701 aagtagacga cgaacagccc gatcccggcg acgacggtca cggccagggc gatcgcggtg
     2761 cggcgttcgg cccggcgctg gctgccggtc agctcccggt tggccggtcc ctgctcggtc
     2821 tggcggtcga cgtccacccg ttcgctcccc tgctggtcgc tgtcggtcat gacttcccgg
     2881 tcgctgctcc ggccggtttc agccgcgccc ggcgatccag cgggaggcca ccaccagcag
     2941 ccccagcccg atcacccagg ccacgaaccc ctccggcacc ggccccttgc cgccgatggg
     3001 ggcgccgccc tcgttgtagg ggtcggtgcg caggctctcg acgtaggcgg cgatgtcgtc
     3061 gacgtcctgc tggctcttgg cggtgccgaa ctcgtagtcg gtcatgccgc cgccgaccgg
     3121 catctgcccg gggccgatct tgatcgcctc ggccacgtcc tgggggtgga cgtcctgcag
     3181 cgacacggcg atgttctcgc cgcccagggc cgccccggcg ccgttcatgc cgtggcaggc
     3241 cgcgcacgcc tggccgtaca gctcgcggcc gcgctggagg tcgccccggg ccgggtcgac
     3301 gtcggggagc tgggcgtcgc cgaccgcctt gccgatgtag acgaccaggg cgtggacgtc
     3361 ctcatcgctg aagcgctgct cgccgcgctc gatggccggg cccttgttgt ccttccaggg
     3421 catccggccg gtgcgcagga cccagttgac ggccgccgcc ccgccgacgt cgttgagcga
     3481 cggcaccgtc gggtagtagc tggggccggc cgggtcgggg ccgtggcagg aggcgcaggc
     3541 gttctcgtac agctcgcggc cgcgggcgtt cagggccggg ctgtcgctgg gctgctgctg
     3601 ggcggcggcc gcggccgccc gccgggacga ggaggccgtc ccggtcgtcg gcgtgaggag
     3661 cagggc
//
LOCUS       DASSWR010000093         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1162612, whole genome shotgun sequence.
ACCESSION   DASSWR010000093 DASSWR010000000
VERSION     DASSWR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162612"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFO47_01505"
     CDS             complement(<1..470)
                     /locus_tag="VFO47_01505"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET8787408.1"
                     /translation="MDPVVPSTGWGVTHLFFRVDSSRSPDPAQAGKELVAALDAFAAA
                     GEDHQVLCASVLGLKADLAVMALGPDLTRHEALARSLRAVSPLEPAYSFLSLTEVSEY
                     METEDDVRARLQAQGVEDTEERVAKALERLAGQREDRLHPRLPRKAVLAFYPMS"
     gene            complement(763..1644)
                     /locus_tag="VFO47_01510"
     CDS             complement(763..1644)
                     /locus_tag="VFO47_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET8787409.1"
                     /translation="MDHTTLIRRPRLRGPVLLAAFDGWNDAGEAATTALDAIGDGLAA
                     ETFAVIDPEEFYDFQATRPTVRQVDGHRRIEWPSIELRAARLPAAEHDLIVVRGHEPN
                     LRWRTFASEIVELAADLRVEMVVTVGALLADVPHTRPVQVVSSAGDRDLAERLGLNVS
                     RYEGPTGILGVLGDVATRQGIPTVGLWAALPHYLNLAPNPWGALALIRELRRLLPMAI
                     DTSTLAGQTEAFDTAVAELVEENPELAGYIERLEADTDDEDEDEEGSPGLADLPPEEL
                     VAEVERYLRDHPGGSRN"
     gene            complement(1873..>2328)
                     /locus_tag="VFO47_01515"
     CDS             complement(1873..>2328)
                     /locus_tag="VFO47_01515"
                     /inference="COORDINATES: protein motif:HMM:NF026109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HET8787410.1"
                     /translation="DVDNGVLVDQQLRAADGVWAAGDVANADNTLFGRIRIEHWAEAL
                     NQGLLAGRNLAGSDGRYDRVPYFFSDQFDLSLSYLGHVREWDELVVRGSREVKEPKFV
                     AWYLREGTPRAALIVNDRDAEDPVREIIRRARPVDAARLADEGVDLGDL"
BASE COUNT          306 a          836 c          852 g          334 t
ORIGIN      
        1 ttgctcatcg ggtagaaggc gagcaccgcc ttgcggggca ggcgcgggtg cagccggtcc
       61 tcgcgctggc cggccagccg ctccagcgcc ttggccaccc gctcctcggt gtcctcgacc
      121 ccctgggcct ggaggcgggc ccggacgtcg tcctcggtct ccatgtactc cgagacctcg
      181 gtgagggaca ggaacgagta cgccggctcc agcgggctca ccgcccgcag cgacctggcc
      241 agcgcctcgt ggcgggtcag gtcgggcccg agggccatca cggccaggtc ggccttgagg
      301 ccgaggacgg aggcgcagag cacctggtgg tcctccccgg ccgcggcgaa ggcgtcgagg
      361 gcggccacca gctccttgcc ggcctgggcg gggtcggggg agcggctgga gtcgacgcgg
      421 aagaacaggt gcgtgacccc ccagccggtc gacgggacga cgggatccat gtggtcgctg
      481 cctcctgggg cgggtgccgg gccgggtccg ttcgccacca tcgtgcctgc tcgcgcggtg
      541 gtttgccgag ttcggcgcag ctgcgacagc gtcctcgacg agatagacaa gtttgagtct
      601 attgacatga ctgaccgggt gctagcatcc ggccgggcaa gggcgcaggc tgccgtcgca
      661 gagcggctcg agctgcccct gcccgccggg tcaggtaccg gcccggcaga gaaccggccc
      721 gcccggccca tccggcccgc cgggccagat cctgttgcgg agctagtttc tcgacccgcc
      781 cgggtggtcg cgcaggtagc gctcgacctc ggccaccagc tcctcgggag ggaggtcggc
      841 gaggccgggc gacccctcct cgtcctcgtc ctcgtcgtcg gtgtcggcct cgagccgctc
      901 gatgtagccg gcgagctccg ggttctcctc gaccagttcg gccacggccg tgtcgaacgc
      961 ctcggtctgg ccggccaggg tgctggtgtc gatggccatc ggcagcagcc gccgcagctc
     1021 ccggatcagc gccaaggcac cccaggggtt gggggccagg ttcaggtagt ggggcagggc
     1081 ggcccacagg ccgaccgtgg ggatcccctg ccgggtggcc acgtcgccga ggacgccgag
     1141 gatcccggtc ggcccctcgt agcgggagac gttcagcccc aggcgctcgg ccaggtcccg
     1201 gtcgccggcc gagctgacca cctggaccgg ccgggtgtgg ggcacgtcgg ccagcagggc
     1261 gccgaccgtg accaccatct cgacccggag gtcggcggcc agctcgacga tctccgaggc
     1321 gaaggtccgc cagcgcaggt tgggctcgtg ccccctgacc acgatcaggt cgtgctcggc
     1381 ggccggcagg cgggcagcgc gcagctcgat ggacggccac tcgatccgcc ggtggccgtc
     1441 gacctgccgc accgttggac gggtcgcctg gaagtcgtag aactcctcgg ggtcgatgac
     1501 ggcgaaggtc tcggccgcca gcccgtcccc gatcgcgtcc agggcggtgg tggcggcctc
     1561 accggcgtcg ttccagccgt cgaaggcggc cagcagcacg gggccccgga gccggggccg
     1621 cctgatcagc gtcgtgtgat ccatgctctc cacgatgcca cggaaggcaa gaggggcgca
     1681 gactggccta cgtagcagag cctacggtgg ccgaggcagg ggggtccgtc gatggggcga
     1741 tcttgctcgc aagagggccg tcgtccccct ctggcacaac acagttcgcg aaaacggcgt
     1801 actatgccca ctgcggcccc tcgcgctgtc ttggcccccc cggatggcgc gggggccctt
     1861 tttctccccg gcctacagat cgcccaggtc aacgccctcg tcggccagcc gggcggcgtc
     1921 caccggccgg gcccggcgga tgatctcgcg gaccgggtcc tcggcgtccc ggtcgttgac
     1981 gatcagggcc gcccgcggcg tgccctcgcg caggtaccag gcgacgaact ttggctcctt
     2041 gacctcgcgg ctgccccgca ccaccagctc gtcccactcc cgcacgtggc ccaggtagga
     2101 cagggacagg tcgaactggt cggagaagaa gtaggggacc cggtcgtagc gtccatccga
     2161 cccggccagg ttgcggccgg ccagcagccc ctggttgagg gcctcggccc agtgctcgat
     2221 gcggatccgc ccgaacagcg tgttgtcggc gttggccacg tcgccggccg cccagacgcc
     2281 gtcggccgcc cgcagctgct ggtcgaccag gacgccgttg tcgacgtc
//
LOCUS       DASSWR010000094         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_569071,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000094 DASSWR010000000
VERSION     DASSWR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569071"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFO47_01520"
     CDS             <1..926
                     /locus_tag="VFO47_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET8787411.1"
                     /translation="GAVVVDEQHRFGVHQRVRLREKGDSPHVLIMTATPIPRTLALTL
                     YGDLDVSTLDELPPGRTPIATHVVADATLRERAYQRVREEAAAGHQAYVVCALKDESD
                     KVEVRSAKAEAERLRGQVFPDLRVGLVYGDMPAREKEATMDRFRAGEVDVLVSTTVIE
                     VGVDVPNATVMLIEDADRFGLSQLHQLRGRVGRGRAPGLCILFADPLTDEGRARMDAI
                     ARTNDGFELANEDLRIRGEGTVFDARQSGLSDLKLARLVEDFDWVRRARADAFALVEA
                     DPELAGPDHRRLRQEVLARLGDGRAEWLARG"
     gene            959..1783
                     /locus_tag="VFO47_01525"
     CDS             959..1783
                     /locus_tag="VFO47_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787412.1"
                     /translation="MSSDDRGWSDEDQLVQYLAEFGFNRNEARIYLAALGRPPLRVAE
                     LAELADVNRPKAYDALRQLVEKGLFEPEGGGQEPARFQATDPMLVVQRLRHQSALEQA
                     DRVQDTALLVADLFARYYEAPQGDDPFEYVELIRNSDAAWARRDAITAGAEKEVVQAR
                     RMTPPGTGPRAGDEVGVREGVSYRALYETGFLDFPQFVARLAEREQRGEQIRFVDKVP
                     VGMTAVDRRKCLLSLNPTGLVSGQGSWVVLEQPALAHLLTDAFDLLWDSARPGPHR"
     gene            1825..2787
                     /locus_tag="VFO47_01530"
     CDS             1825..2787
                     /locus_tag="VFO47_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787413.1"
                     /translation="MRLAPVVPSGKDRSLTQSDDSDLIDRLVRHGLGQGEARCYVGML
                     AQRAFKVSEVAAEAGLPRSRAYELIRSLVRFGLCTEVAGSGVARFRAVPPNEAIGRLE
                     ASKAEQARRRDLALNGLAEALTLRAGPTPGAGEEPLEMLRRREQLLGVMATEVCRARQ
                     EVLTIVNTSWEPADCPGMRDRLAAGVRFRTIFDRDSLEHDPHREWVAAFADDDGFEVR
                     VVDRVETLYLLVDRYVVLLNLTVPGSGSPGSHAESLLMRHAGLGEILHDAFERTWVQG
                     VPFEQALEADGAPALVGATASARGHAPIRWRGSHASNGRSASSG"
     gene            2872..3438
                     /gene="rsmD"
                     /locus_tag="VFO47_01535"
     CDS             2872..3438
                     /gene="rsmD"
                     /locus_tag="VFO47_01535"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET8787414.1"
                     /translation="MRVVGGRLGGRRLRAVPGRDTRPTSDRVREALFGVLGDRVEGAR
                     VLDLFAGTGALAIEALSRGAASAVLVEQAAPAVTVIRANLDALGLGGVATVRRTRAET
                     YLRTQRDGPFDLVLLDPPYAAPVGLVAGLLGRLARTALAPGAVVVVEAAARAEAPPWP
                     PGLTPERPRRYGDTALHLAILDAPGGSA"
     gene            3505..3990
                     /gene="coaD"
                     /locus_tag="VFO47_01540"
     CDS             3505..3990
                     /gene="coaD"
                     /locus_tag="VFO47_01540"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET8787415.1"
                     /translation="MRTACCPGTYDPVTNGHLDIVARASRAFDRVIVAVLINPAKQPM
                     FTLEERLAMLKEATVGMASVEIDAFSGLLVDYARRREVTAIVKGLRAVSDFDYELQMA
                     QMNYRLAAVDTMFMTTNPTWSYLSSSLVKDVARHGGDVTGLVPDFVVTRLRERAAQPP
                     A"
BASE COUNT          551 a         1638 c         1468 g          566 t
ORIGIN      
        1 tgggggcggt ggtggtcgac gagcagcacc gcttcggcgt gcaccagcgg gtccggctgc
       61 gcgagaaggg cgacagcccc cacgtcctca tcatgaccgc gaccccgatc ccgcggacgc
      121 tcgccctgac cctctacggc gacctggacg tgtccaccct ggacgagctg ccgccgggcc
      181 ggaccccgat cgccacccac gtggtcgccg acgccaccct gcgggagcgg gcctaccagc
      241 gggtccgcga ggaggctgcg gccggccacc aggcctatgt cgtctgcgcc ctcaaggacg
      301 agtccgacaa ggtcgaggtc cgctcggcca aggccgaggc cgagcggctc cggggccagg
      361 tcttccccga cctgcgggtc gggctggtct acggcgacat gccggccagg gagaaggagg
      421 cgaccatgga ccgcttccgg gccggcgagg tcgacgtgct cgtctccacc accgtgatcg
      481 aggtcggcgt cgacgtgccc aacgccaccg tgatgctgat cgaggacgcc gaccgcttcg
      541 gcctgtccca gctccaccag ctccgggggc gggtcgggcg gggccgggcc cccgggctgt
      601 gcatcctgtt cgccgacccc ctgaccgacg agggccgggc ccgcatggac gccatcgccc
      661 gcaccaacga cggcttcgag ctggccaacg aggacctgcg catccgcggc gagggcaccg
      721 tgttcgacgc ccgccagtcg ggcctctccg acctcaagct ggcccgcctg gtcgaggact
      781 tcgactgggt ccggcgggcc cgggccgacg ccttcgcgct ggtcgaggcc gacccggagc
      841 tggccggccc cgaccaccgg cggctgcgcc aggaggtgct ggcccgcctc ggcgacggcc
      901 gcgccgagtg gctggcccgc gggtagcaca aaccccccat acgcactacg ctcccgccat
      961 gtcgagcgac gaccgtggct ggtcggacga ggaccagctc gtccagtacc tggccgagtt
     1021 cggcttcaac cgcaacgagg cccggatcta cctggccgcc ctcggccgtc cgccgctgcg
     1081 ggtcgccgag ctggccgagt tggccgacgt caaccggccc aaggcctatg acgcgctgcg
     1141 ccagctggtc gagaaggggc tgttcgagcc ggagggtggc ggccaggagc cggcccggtt
     1201 ccaggccacc gacccgatgc tggtcgtgca gcgcctgcgc caccagagcg cgctcgagca
     1261 ggccgaccgg gtccaggaca ccgcgctgct ggtcgccgac ctgttcgccc gctactacga
     1321 ggccccccag ggcgacgacc cgttcgagta cgtggagctg atccgcaact ccgacgccgc
     1381 ctgggcccgc cgcgacgcca tcaccgccgg cgccgagaag gaggtcgtcc aggcccgccg
     1441 gatgaccccg ccggggaccg ggccgcgggc cggcgacgag gtcggcgtcc gcgagggggt
     1501 cagctaccgg gccctgtacg agaccgggtt cctcgacttc ccgcagttcg tggcccggct
     1561 ggccgagcgc gagcagcgcg gcgagcagat ccgcttcgtc gacaaggtgc cggtcggcat
     1621 gaccgccgtc gaccgccgca agtgcctgct cagcctcaac ccgaccgggc tggtcagcgg
     1681 gcaggggagc tgggtcgtgc tggagcagcc ggccctggcc cacctgctca ccgacgcctt
     1741 cgacctgctg tgggactcgg ccaggccggg tccccaccgg tagcagctca accttcttga
     1801 cttcgccctg aaccgtgcct actgttgcgg ctcgctcctg ttgtcccctc tgggaaggac
     1861 agatcgctga cccagtccga tgactcggat ctgatcgacc ggctcgtccg acatggcctc
     1921 ggccagggcg aggcccgctg ctacgtcggc atgctggccc agcgggcgtt caaggtcagc
     1981 gaggtcgccg ccgaggccgg cctgcccagg tcacgggcgt acgagctgat ccggtcgctg
     2041 gtccgcttcg ggctctgcac cgaggtggcc ggcagcgggg tcgcccgctt ccgggccgtc
     2101 ccgcccaacg aggccatcgg ccggctggag gcgtccaagg ccgagcaggc ccgccggcgc
     2161 gacctggccc tcaacggcct ggccgaggcg ctcaccctcc gcgccggccc gacacccggg
     2221 gccggcgagg agccgctgga gatgctgcgc cgccgcgagc agctcctcgg cgtcatggcc
     2281 acggaggtct gccgggcccg ccaggaggtg ctgacgatcg tcaacacctc ctgggagccg
     2341 gccgactgcc cggggatgcg cgaccgcctg gccgccgggg tccgcttccg caccatcttc
     2401 gaccgcgact ccctggagca cgacccgcac cgggagtggg tggccgcctt cgccgacgac
     2461 gacgggttcg aggtccgggt cgtcgaccgc gtcgagaccc tgtacctgct cgtcgaccgg
     2521 tacgtcgtgc tcctcaacct gaccgtcccg ggctccgggt cgcccggcag ccatgccgag
     2581 agcctgctga tgcgccacgc cggcctcggc gagatcctcc acgacgcctt cgagcgcacc
     2641 tgggtgcagg gcgtcccttt tgagcaggcc ctcgaggcgg acggcgcacc cgccctcgtg
     2701 ggcgcaacgg ccagcgcgcg cggccacgcc ccgatccggt ggcgcggttc gcacgccagc
     2761 aacggccgct cggcctcgtc tggctgagcc ggcccgaccg gttggacggc gggggaacgg
     2821 ccttggcccc cccgggctaa gtaccccgcc gccagccact caggggggag cgtgcgggtc
     2881 gtcggaggcc gcctcggcgg ccgccgcctg cgggccgtcc cggggcggga cacgcggccg
     2941 accagcgacc gggtcaggga ggcgctgttc ggggtcctcg gcgaccgggt cgagggggcg
     3001 cgggtgctcg acctgttcgc cgggaccggg gcgctggcca tcgaggcgct gtcgcgcggg
     3061 gccgcctcgg cggtgctggt cgagcaggcc gccccggcgg tgacggtgat ccgggccaac
     3121 ctggacgccc tcggcctggg cggcgtggcc acggtgcgcc gcacccgggc cgagacctac
     3181 ctccggaccc agcgggacgg gcccttcgac ctggtcctgc tcgacccgcc ctatgcggcc
     3241 ccggtcggcc tggtcgccgg cctgctcggc cgcctggccc gcaccgccct ggccccgggt
     3301 gcggtggtcg tggtcgaggc cgccgcccgc gccgaggccc cgccctggcc gccgggcctg
     3361 acgcccgaac gcccccgccg ctacggcgac accgcgttgc acctcgccat ccttgatgct
     3421 ccggggggct cggcttgagc cccccgggcc cccaggtggg tacccttgac ctgagcccga
     3481 cccccagcca ggagaccccg acccgtgcgg accgcttgct gccccggcac ctacgacccg
     3541 gtcaccaacg gccacctcga catcgtggcc cgggccagca gggccttcga ccgggtgatc
     3601 gtggccgtcc tcatcaaccc ggccaagcag ccgatgttca ccctcgagga gcgcctggcc
     3661 atgctcaagg aggccaccgt tgggatggcc tcggtcgaga tcgacgcctt ctccggcctg
     3721 ctggtcgact acgcccgccg ccgggaggtc accgccatcg tcaagggcct gcgagccgtc
     3781 tccgacttcg actacgagct ccagatggcg cagatgaact accgcctggc cgctgtcgac
     3841 accatgttca tgaccaccaa cccgacctgg tcgtacctga gctccagcct ggtcaaggac
     3901 gtggcccgtc acggcggcga cgtcaccggc ctggtccccg acttcgtggt gaccaggctg
     3961 cgggaacgcg ccgcccagcc cccggcctag cccgcgctca ccggccactt tctgctaccc
     4021 tgatatgctg cggtggctcc ccgccgcccg cttttgtgtg ccctttacac accccacgcg
     4081 gcacatcttg agaatcgcca tttccgatga gcaaacacgt cacagaaccg gcactcgcgg
     4141 tcgacgtccg cgagctgctc cagagacccg gagcccacaa gcacgtcgtc gtccgcgcgc
     4201 cccttgagga cctggccacc ccg
//
LOCUS       DASSWR010000095         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_371365,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000095 DASSWR010000000
VERSION     DASSWR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371365"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..968
                     /locus_tag="VFO47_01545"
     CDS             285..968
                     /locus_tag="VFO47_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787416.1"
                     /translation="MRAHELVETGRQHVATGRIREAMACFKRAADDPERGAEALSLLA
                     AAYLLPGSPAPELALAHAHRAASHPAAGGEHLARCAAVALTAGDPSLAEELAGRAGRA
                     GGGEALAIRATALLRLGDLAEVRALLAGLERASGPAVFWRRLVVEASAAGRLDIAFTV
                     RRAMRRAGLRAPAVPDVLVRAMAGPLFFACLATASVLAVAVPNQVAAVVALGFLAVGL
                     GTAVLARRG"
     gene            1092..>2281
                     /locus_tag="VFO47_01550"
     CDS             1092..>2281
                     /locus_tag="VFO47_01550"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HET8787417.1"
                     /translation="MPAASVAIQGRLDDLGTPLLDVEFVVLDLETTGGSPANDRITEV
                     GAVKIRGGEVLGTFHTLVNPEVSIPPLISALTGITNGMVADAEPIEVVLPCLLEFLGG
                     AVLVAHNASFDRRFVQANLERHGYQRLANRVVCTARLARKLLPRDEVPNVRLATLAAY
                     LGATVAPCHRALTDARATVDVFHGLLERAGSYGVLALEDLIEFPSAKASANFKKVHLA
                     DRLPRCPGVYLFRDAAGRVLYVGKAKDLRTRVRSYFSGDGRVKVADLLRELAAIDHQP
                     CATELEASVREVRMIQHHRPRYNRRGRNPERYCYLKLTRERFPRLSLVRRVQADGARY
                     LGPFGSTGQAELVKSAIEDALPLRRCTMRIGARGGGSACALLELGRCLGPCTGAVGRE
                     RYAAL"
BASE COUNT          250 a          936 c          794 g          301 t
ORIGIN      
        1 tcccgggccc ccacccggga gggtagcccg gccggggcgt tggtgcgctg gctcaccaca
       61 gccccccgca gcccactcca cccgcaccca gctgccgccc ccgcccgtcc gccccggggc
      121 gccggcccgc ggtcggcgac ctcgcaccgc tccggctcgg cgctcccaaa gtcgagccga
      181 acggcccccg cgtccggttt gtccccctgc cgcggcgagc tggctttcgc ttacgattgc
      241 gatcaccgcg gccctcgcgg tggccgacag gtaggaggcg cgccgtgcga gcgcacgagc
      301 tggtcgaaac cggtcgccag cacgtggcca ccggccggat ccgcgaggcg atggcctgct
      361 tcaagcgggc cgcggacgac cccgaacgtg gcgccgaggc gctgagcctg ctcgccgccg
      421 cctacctgct gcccgggtcg ccggcccccg agctcgccct cgcccacgcc caccgcgccg
      481 ccagccaccc ggccgcgggc ggcgagcacc tggcccgctg cgccgccgtc gccctcaccg
      541 ccggcgaccc gtcgctggcc gaggagctgg ccggccgcgc cggccgcgcc ggcggcggcg
      601 aggccctggc catccgggcc accgccctgc tgcgcctcgg cgacctggcc gaggtgcggg
      661 ccctgctcgc ggggctggag cgggcgtcgg gcccggcggt gttctggcgg cggctggtgg
      721 tggaggcgtc ggcggccggc cggctcgaca tcgccttcac cgtccgccgg gccatgcgcc
      781 gcgccggcct ccgcgccccc gccgtccccg acgtcctcgt ccgggccatg gccgggccgc
      841 tgttcttcgc ctgtctggcc accgcctccg tgctggccgt ggccgtgccc aaccaggtgg
      901 ccgcggtggt cgcgctcggg ttcctcgccg tcggcctcgg cacggccgtg ctggcccggc
      961 gcggctgacc agccggtccg ctgggcgccc ctgcgccctt ctccccaggt ggcctcgagg
     1021 tgcttgacct cgaacacctg ttcgtctagc gtcgcctcga cagcccagag cggacgaagg
     1081 gaaccgcagg gatgcccgcc gcatccgtcg ccatccaggg tcgtctcgac gacctcggca
     1141 ccccgctgct cgacgtcgag ttcgtcgtgc tcgacctgga gaccaccggc gggtcgcccg
     1201 cgaacgaccg gatcaccgag gtcggcgccg tcaagatccg cggcggcgag gtcctcggca
     1261 ccttccacac cctggtcaac cccgaggtct cgatcccgcc cctgatcagc gccctgaccg
     1321 gcatcaccaa cgggatggtg gccgacgccg agccgatcga ggtcgtactc ccctgcctgc
     1381 tcgagttcct cggcggcgcc gtgctggtcg cccacaacgc ctccttcgac cggcgcttcg
     1441 tccaggccaa cctggagcgc cacggctacc agcgcctggc caaccgggtc gtctgcacgg
     1501 cccggctggc ccgcaagctc ctcccccgcg acgaggtgcc caacgtccgg ctggccaccc
     1561 tggccgccta cctcggggcg acggtcgccc cctgccaccg ggccctgacc gacgccaggg
     1621 ccaccgtgga cgtattccac ggtctgctgg agcgggccgg gtcctacggc gtcctcgccc
     1681 tcgaggacct gatcgagttc ccctcggcca aggcgagcgc caacttcaag aaggtccacc
     1741 tggccgaccg gctcccccgc tgcccggggg tctacctgtt ccgcgacgcc gccgggcggg
     1801 tcctgtacgt cggcaaggcc aaggacctcc ggacccgggt ccgcagctac ttctcgggcg
     1861 acggacgggt caaggtcgcc gacctgctgc gcgagctggc cgcgatcgac catcagccgt
     1921 gcgcgaccga gctggaggcg agcgtccgcg aggtccggat gatccagcac caccggcccc
     1981 gctacaaccg ccggggccgc aaccccgagc gctactgcta cctgaagctg acccgcgagc
     2041 ggttcccgcg gctgtcgctg gtgcggcggg tccaggccga cggggcccgc tacctgggcc
     2101 cgttcggctc gaccgggcag gcggagctgg tcaagtcggc gatcgaggac gccctgccgc
     2161 tgcgccgctg caccatgcgg atcggggcgc gggggggtgg gtcggcctgt gccctgctgg
     2221 agctgggccg ctgcctcggc ccctgcaccg gcgcggtcgg gcgggagcgg tacgccgccc
     2281 t
//
LOCUS       DASSWR010000096         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_653984,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000096 DASSWR010000000
VERSION     DASSWR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653984"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFO47_01555"
     CDS             complement(<1..538)
                     /locus_tag="VFO47_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787418.1"
                     /translation="MTLLRFSLGQLLAGRRVVIVALLVALPLVLPAVFAAGADIDPAT
                     FTLDLFRQLVLPVLLPVVALTFSTSSLGSELRDGTVTNLLLKPIPRPAVLGAKYLAAV
                     LSSLLVLLPAEAVGHVVAAGGLGSTDLLAGMLLATTVGTLAYCALGVLLSLLMARALL
                     VGLAYALLWEGAVVSVAPS"
     gene            complement(547..1485)
                     /locus_tag="VFO47_01560"
     CDS             complement(547..1485)
                     /locus_tag="VFO47_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787419.1"
                     /translation="MSGPAPNGAPVVELRRFSKWYGDVVAVADLSFELRPGVTGLLGP
                     NGAGKTTTLKAICGLLHPSQGEVLLFGRPIRNDPMLYRAIGVVPDGDRLYPRLTIRSY
                     VRLHAELQGLADAGAAAERAIAEVDLQPLADRRLKGLSKGERQRTKVAGAIVHQPALL
                     VLDEPMTGMDPGQRARFIELVRLIGEAGVTVLVSSHILGEVERLASQILVLVSGRLAA
                     SGDYHAIRDLMADQPRTVMVKADDARLLAAGLIALPSTVAVEVDGDALRARTRSVDSF
                     ATALPRLARDQGATLREVRTTDESLEQVFRYLVGRR"
     gene            complement(1482..2375)
                     /locus_tag="VFO47_01565"
     CDS             complement(1482..2375)
                     /locus_tag="VFO47_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787420.1"
                     /translation="MTELTTPAGTIYDRGYRHYEGPREGRGRRMRAIAVAGVRRVLGL
                     KRNWKTKVVPWGLLVLAFGPVLAFIGVRVLFAEAADEFFTYAQYLRIVAGLLLLFAAT
                     AGPELLCPDRRQNVLALIFTRPVTRADYLLAKLAALLAVVGLIAIVPLVVLFLGNTLT
                     AVSAATYLRENLDDLGKILIAGTALTIFYSVVALAAASLTDRRAVATAGLLGVFLGSS
                     ALANILFFTAQFEGRRWVAFLSLSGLPGRFVDWLFGAPFDPGTLADQAGFTGPGYLVA
                     MTVVAAVAGAILTWRVLRLRA"
BASE COUNT          383 a          889 c          904 g          261 t
ORIGIN      
        1 ccgagggcgc gaccgagacg accgccccct cccacagcag ggcataggcc agcccgacca
       61 gcagcgccct ggccatcagc agcgacaaca gcaccccgag ggcgcagtag gcgagcgtgc
      121 cgacggtggt ggccagcagc atgccggcga gcaggtcggt cgagccgagc ccgccggcgg
      181 cgaccacgtg cccgaccgcc tcggcgggta gcaggaccag cagcgacgac agcacggcgg
      241 ccaggtactt ggccccgagc acggccgggc gcgggatcgg cttgagcagg aggttggtga
      301 ccgtgccgtc gcgcagctcc gagccgaggc tcgaggtcga gaaggtgagg gcgaccaccg
      361 ggagcagcac gggcaggacc agctggcgga acaggtcgag ggtgaaggtg gccgggtcga
      421 tgtcggcccc ggccgcgaac accgccggga gcaccagcgg cagcgcgacc agcagggcga
      481 cgatgaccac ccgccgtccg gccagcagct ggccaaggct gaaccgcagc aaggtcatgg
      541 atctgctcat cgtctcccca ccagataccg gaacacctgc tcaagggact cgtcggtggt
      601 gcggacctcg cgcagggtgg ccccctggtc gcgggccagc cggggcaggg cggtggcgaa
      661 gctgtccacc gagcgggtgc gggcccgcag ggcgtccccg tcgacctcga cggccaccgt
      721 cgacggcagg gcgatcagcc cggcggccag cagccgggcg tcgtcggcct tgaccatgac
      781 cgtgcgcggc tgatcggcca tgaggtcgcg gatggcgtgg tagtcgccgg aggcggccag
      841 ccggcccgac accagcacca ggatctggga ggcgagccgc tccacctcgc cgaggatgtg
      901 gctggacacc agcacggtca ccccggcctc gccgatcagc cggaccagct cgatgaagcg
      961 ggcccgctgc cccgggtcca tgccggtcat cggctcgtcc agcaccagca gggccggctg
     1021 gtggacgatc gcccccgcga ccttggtgcg ctggcgctcg cccttggaca gtcccttgag
     1081 gcgccggtcg gccagcggct ggaggtcgac ctcggcgatg gcccgctcgg cggccgcccc
     1141 ggcgtcggcc agcccctgga gctcggcgtg cagccggaca taggagcgga tggtcagccg
     1201 cgggtagagc cggtccccgt cgggcaccac cccgatggcc cggtacagca tcgggtcgtt
     1261 gcggatgggc cgcccgaaca gcagcacctc gccctgggag ggatgcagca gcccgcagat
     1321 ggccttgagg gtggtggtct tgccggcccc gttgggtccc agcagcccgg tcacccccgg
     1381 ccgcagctcg aacgacaggt cggccacggc gaccacgtcc ccgtaccact tcgagaaccg
     1441 ccgcagctcg accacgggag cgccgttcgg cgccgggccg ctcatgcccg caggcgcagg
     1501 acgcgccagg tcaggatggc cccggccacc gcggccacca ccgtcatcgc caccaggtac
     1561 ccgggcccgg tgaagccggc ctggtcggcc agggtgcccg ggtcgaacgg ggccccgaac
     1621 agccagtcga cgaagcgccc gggaaggccg ctgagcgaca ggaacgccac ccagcgccgc
     1681 ccctcgaact gggcggtgaa gaacaggatg ttggccaggg ccgaactgcc gaggaacacc
     1741 ccgagcagcc cggccgtggc caccgcccgc cggtcggtga ggctggccgc ggccagggcg
     1801 acgaccgagt agaagatcgt cagcgccgtc ccggcgatca ggatcttgcc caggtcgtcg
     1861 aggttctcgc gcaggtaggt ggcggcgctc acggcggtca gggtgttgcc caggaagagc
     1921 accaccaggg gcacgatcgc gatcagcccg accacggcca gcagcgcggc cagcttggcc
     1981 agcaggtagt cggcgcgggt gaccggccgg gtgaagatca gggcgagcac gttctggcgg
     2041 cggtcggggc agagcagctc cggtccggcg gtggccgcga acagcagcag caggccggcg
     2101 acgatgcgca ggtactgggc gtaggtgaag aactcgtcgg cggcctcggc gaacagcacg
     2161 cggacgccga tgaaggccag caccggcccg aaggccagca ccagcagccc ccacgggacc
     2221 accttggtct tccagttgcg cttgagcccc agcacccggc gcaccccggc gacggcgatg
     2281 gcccgcatcc gccgcccgcg gccctcccgc ggcccctcgt agtgccggta gccgcggtcg
     2341 tagatggtcc ccgcgggggt cgtgagctcc gtcatcgccg caccccccag gccgcctcgg
     2401 gcgtgcgcgc cgcctcggcc tcccgggtca cggggcc
//
LOCUS       DASSWR010000097         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_767127,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000097 DASSWR010000000
VERSION     DASSWR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767127"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..847
                     /locus_tag="VFO47_01570"
     CDS             53..847
                     /locus_tag="VFO47_01570"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8787421.1"
                     /translation="MYLQGIGQVKVSVHRQVKGRVKTIQVRREGRRWMLILSCDEVPT
                     RPLEPTQAAVGVDVGIASFATTSDGQCVPNPGYGRVAAARLATAQQALARKRRRSTNR
                     RRASDTLAARHRKVANQRRNFHHQTARTLVARYGLLVVEDLRIRNLVRRPASRPDPDQ
                     PGGFRPNGAAAKSGLHRSIHDVGWAQFVSILRAKAEEAGRVVIDVDARHTSDRCEACG
                     HTATENRASQAVFSCRRCGHTVNADEHAARNILRAGLAHLAATPAA"
     gene            complement(888..>2234)
                     /locus_tag="VFO47_01575"
     CDS             complement(888..>2234)
                     /locus_tag="VFO47_01575"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET8787422.1"
                     /translation="LAAEVLETLRVMAELQGRYGAEACRRYVVSFSRSAADLAAVRAL
                     ARLAVPDGPLELDVVPLFESRADLERACDVLDEYLALPGAAAWLERRGRRLEVMLGYS
                     DSAKDAGFLAANLALYRAQGGLAAWAAARGIDLVLFHGRGGALGRGGGPAGRAVRGQA
                     PGSVAGRFKVTEQGEVIYARYGNLAIGHRHLEQVTNAVLGASTPGAQAAVAAAEDRYL
                     ATATAMAAAAEAAWRRLVERPGFAEFFARVTPIKELGLLRIGSRPARREGAGAGADGD
                     DLVDLRAIPWVFAWSQNRCNLPGWYGLGAGLEVVAHQPQGLALLREMYDRWPFFRSLI
                     ENAEMSLAKADPLVAESYLDLGGRPDLVAAIREEFRRTRVLVTETTGAGRLLGHRPVL
                     RQAVDLRNPYVDALSFLQVRFLTDLRNGLTGEEAARAAGLVLLTVNGVAAGLQNTG"
BASE COUNT          326 a          821 c          819 g          268 t
ORIGIN      
        1 ggaatggccc agggacggcg atggctgccg ctggcagctc gacacccgca gggtgtacct
       61 gcaagggatc gggcaggtga aggtcagcgt ccaccgtcag gtcaaaggca gggtcaagac
      121 gatccaggtt cgccgcgaag ggcgccgctg gatgctgatc ctgtcttgtg acgaggtgcc
      181 cacccggccc ctggaaccca ctcaggccgc ggtcggggtc gacgtcggga tcgccagctt
      241 cgccaccact agcgacggcc agtgtgtgcc caatcctggc tacgggcggg tcgccgccgc
      301 caggctggcc accgcccaac aggccctggc ccgtaaacgg cggaggtcga ccaaccgtcg
      361 tagggccagc gacacgctgg cggctcgtca tcgcaaggtt gccaaccagc gccgcaactt
      421 ccaccaccag acggccagga cgctcgtcgc ccgctacggc ctgctggtcg tggaggacct
      481 gcggatccgc aacctggtcc ggcgaccggc ttcccgtccc gatcctgacc agccgggcgg
      541 gttccggccc aacggggccg ccgccaagtc cggcttgcac cgaagcatcc atgacgtggg
      601 ctgggctcag ttcgtgtcga tcctgcgcgc caaagcggaa gaggctggcc gtgtggtgat
      661 cgacgtcgac gcccgccaca cctctgaccg ctgcgaagcc tgcgggcaca cagcaaccga
      721 gaaccgcgcc agccaggccg tgttctcctg tcgacgttgc gggcacacgg tcaacgccga
      781 cgaacatgcg gcacgcaata tcctccgggc cggactggcc catctcgcag ccaccccggc
      841 tgcgtgagaa gaagctggca gctccagccg tcagagaagt caccgcctca gccggtgttc
      901 tggaggccgg cggcgacccc gttgacggtg agcaggacca gtccggccgc cctggccgcc
      961 tcctccccgg tgaggccgtt gcgcaggtcg gtgaggaagc ggacctggag gaacgacagg
     1021 gcgtcgacgt aggggttgcg gaggtcgacg gcctggcgca gcaccggccg gtggccgagg
     1081 agccgccccg ccccggtggt ctcggtgacc agcacccggg tgcgccggaa ctcctcgcgg
     1141 atggccgcga ccaggtcggg ccggccgccc aggtccaggt aggactcggc caccagcggg
     1201 tcggccttgg ccaggctcat ctcggcgttc tcgatcagcg accggaagaa cggccagcgg
     1261 tcgtacatct cccgcagcag ggcgaggccc tggggttgat gggcgacgac ctccagcccg
     1321 gccccgagcc cgtaccagcc gggcaggttg cagcggttct ggctccaggc gaacacccac
     1381 gggatggccc gcaggtcgac caggtcgtcc ccgtccgccc cggccccggc gccctcgcgg
     1441 cgggccgggc gggacccgat ccgcagcagg cccagctcct tgatcggggt caccctggcg
     1501 aagaactcgg cgaaccccgg ccgttccacc agccggcgcc aggccgcctc ggcggcggcg
     1561 gccatggccg tggccgtggc caggtagcgg tcctcggcgg cggccacggc ggcctgggcg
     1621 ccgggggtgg aggcgccgag gacggcgttg gtgacctgct ccaggtggcg gtggccgatg
     1681 gccaggttgc cgtagcgggc gtagatgacc tcgccctgct cggtgacctt gaagcgcccg
     1741 gcgaccgagc ccggggcctg gccgcggacc gcccggccgg ccgggccgcc gccgcggccg
     1801 agggcgccgc cgcggccgtg gaacaggacc aggtcgatgc cgcgggccgc cgcccaggcg
     1861 gccagtccgc cctgggcccg gtacagggcc aggttggcgg cgaggaagcc ggcgtccttg
     1921 gccgagtccg agtagccgag catgacctcc aggcgccggc cccggcgctc cagccaggcg
     1981 gccgcgcccg ggagggccag gtactcgtcc aggacgtcgc aggcccgctc caggtcggcc
     2041 cgggactcga acagcggcac cacgtccagc tccagcgggc cgtcgggcac ggccagccgg
     2101 gccagggccc ggaccgcggc caggtcggcg gccgagcggc tgaagctgac cacataccgc
     2161 cggcaggcct cggccccgta gcggccctgg agctcggcca tgacccgcag ggtctccagc
     2221 acctcggcgg cgag
//
LOCUS       DASSWR010000098         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1162674, whole genome shotgun sequence.
ACCESSION   DASSWR010000098 DASSWR010000000
VERSION     DASSWR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162674"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..555
                     /locus_tag="VFO47_01580"
     CDS             190..555
                     /locus_tag="VFO47_01580"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET8787423.1"
                     /translation="MTTISEAELFHALPGWQRYGNRIEKTFTFDDFGAAARFVGRMAD
                     DAAVAGRQPAIDLRCDCVTVAFAPRDGSTVTLDDVAVASRIQRLLGDHRHPVGRAGPW
                     SPGGLLACRRPRPVGQAGP"
     gene            complement(678..1385)
                     /locus_tag="VFO47_01585"
     CDS             complement(678..1385)
                     /locus_tag="VFO47_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787424.1"
                     /translation="MDPNDVDQQLTSLAGLGDPIRRALYRYVADRGAPVSRDEAAQAA
                     GISRPLAAYHLDKLVEDGLLEPRYHRRGRRRGPGAGRPAKHYVRADRPLELSLPARDY
                     AALAELLAGAFEADRSGAAKAALNRAAAALGAELGTEAAGRTAPDGDPDQVLAAVRQV
                     LAARGYEPYQDPDGTIRLRNCPFDRIAARHRELVCGANHAMLQALTHQVDGDPPTVRA
                     VLDPQPGRCCVTLTRAS"
     gene            1471..1905
                     /locus_tag="VFO47_01590"
     CDS             1471..1905
                     /locus_tag="VFO47_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787425.1"
                     /translation="MTTINTVRSHNTSVRDNPAYQAYLTLRVGFVVAPILFGLDKFTN
                     LLTDWTAYLAPAIDRLVPGSATGAMLAVGVVEIVAGLVVAVRPKIGGYLVAAWLAGII
                     TNLLLLGEHYDVALRDFGLLLAALALARLATAFQPTHDTDAA"
     gene            2005..2622
                     /gene="folE"
                     /locus_tag="VFO47_01595"
     CDS             2005..2622
                     /gene="folE"
                     /locus_tag="VFO47_01595"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET8787426.1"
                     /translation="MPEEPTPLGSRPKLELVGRSPAVDLVRAERAVAELLAALGQDPT
                     SEELAETPRRVAASYAELLTPAPFTLTSFPNDEGYDELVLARAIPFSSLCQHHLLPFT
                     GVAHVGYLPAGRILGLSKLARVVELFARRLQLQERLTTQVADWLQTQLEPKGVGVVLE
                     AEHLCMTLRGVQATGARTVTSALHGRLRDDPRSRQEFLALTRTAT"
     gene            2650..>3129
                     /locus_tag="VFO47_01600"
     CDS             2650..>3129
                     /locus_tag="VFO47_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8787427.1"
                     /translation="MTATPTSTFVIVGAGLAGAKAAETLRAEGFDGRLLLLGEEPERP
                     YERPPLSKAYLRGEADRDSLYVHQEGFYAAHDIELRPSTPVRSIDPGGRQLELASGER
                     IGYERLLLATGATPRQLPLPGADLAGVQYLRTRQDADVLAAAAARAEQVVVVGTGWIG
                     "
BASE COUNT          427 a         1155 c         1097 g          450 t
ORIGIN      
        1 ttgcgaccct ggcttacacg ccgtttcgct cccttggggt cgatctggca ggatcgtggt
       61 gaatagggtt gtccatgaag tacaacttat cttgctggcc aaacaattaa gtggtactct
      121 ccttgtggat cctccgtatg acatcgacga ccacgagcga ccgtcgcgga ggagcgcaag
      181 gaggccacca tgaccaccat cagcgaggcg gagctcttcc acgccctgcc cggctggcaa
      241 cgctacggca accgcatcga gaagaccttc accttcgacg acttcggtgc tgcggcgcgg
      301 ttcgtcggcc ggatggccga tgacgctgcc gttgccggtc gccagcctgc catcgacctc
      361 cgctgcgatt gcgtcaccgt tgccttcgcc ccacgggacg gcagcactgt gaccctggac
      421 gatgtggccg tggcaagccg catccagcgg ctcctcggcg accaccgcca cccggtcggc
      481 cgggccggcc cctggagtcc cggcgggctg ctggcctgca ggcgcccgcg tccagttggt
      541 caggccggcc cctgaacgcg gccgggtcac ttgcgacatg aggtcgtgcg acgtcggcac
      601 gcacacccgc ccatccggtc cggtggtgtg ctgagaccca acagtggcgc tcgcccgcga
      661 tagcccggat cggccgctca gctggcgcgg gtcaaggtga cgcagcagcg tccgggctgt
      721 gggtccagca ccgcccggac ggtcggcggg tcgccgtcga cctggtgggt gagggcttgg
      781 agcatggcgt ggttggctcc gcagaccagc tcgcggtgcc gggcggcgat gcggtcgaag
      841 gggcagttgc gcagccggat ggtgccgtcg gggtcctggt agggctcgta gccgcgggcg
      901 gctagcacct ggcggacggc ggcgaggacc tggtccgggt cgccgtcggg cgcggtccgg
      961 ccggccgcct cggtgccgag ctcggcgccc aaggcggcgg cggcgcggtt gagggcggcc
     1021 ttggcggccc cggagcggtc ggcctcgaag gcgccggcga ggagctcggc cagggcggcg
     1081 tagtcgcggg cgggcaggct gagctcgagc gggcggtcgg cgcggacgta gtgcttggcc
     1141 gggcggccgg cgcccggccc gcggcggcgc ccgcgccggt ggtagcgggg ctccagcagg
     1201 ccgtcctcga cgagcttgtc gaggtggtag gcggccaggg gccggctgat gccggcggcc
     1261 tgggcggcct cgtcccggct gacgggggcg ccgcggtcgg ccacgtaccg gtagagggcg
     1321 cggcggatgg ggtcgcccag gccggccagg ctggtgagct gctgatcaac gtcattagga
     1381 tccatacgtc cattctaaaa caaaagattc ttgacagcta gcctcagccc gtctataaca
     1441 aagataggct agtgatagag gaggaagggc atgaccacca tcaacaccgt ccgttctcac
     1501 aacacgtccg tccgcgacaa ccccgcctac caggcctacc taaccctgcg ggtcgggttc
     1561 gtggtggccc cgatcctgtt cgggctggac aagttcacca acctgctgac ggactggacc
     1621 gcctacctgg caccggccat cgaccgcctg gtcccaggca gcgccaccgg cgcgatgctg
     1681 gccgtcggcg tcgtcgagat cgtcgctggg ctggtcgtgg ccgtccggcc caagatcggc
     1741 ggctacctgg tggccgcctg gctggccggg atcatcacca acctgctgct gctcggcgag
     1801 cactacgacg tcgccctgcg cgacttcggc ctgctgctgg ccgccctggc cctggcccgg
     1861 ctcgccaccg ccttccagcc cacccacgac accgacgccg cctgacgacg cccgcagagc
     1921 gggcggcgtt gccgaccagc caacgccgcc cgcctgcgca ctcgacccct gaaaggatcc
     1981 gacgtgtccg ctgccgccca cctcatgccc gaggagccca caccgctcgg cagccgcccg
     2041 aagctggaac tcgtgggccg ctccccagcg gtcgacctgg tccgggccga gcgggcggtg
     2101 gccgagctgc tggccgcgct cggccaggac ccgacctcgg aggagctggc ggagacgccg
     2161 cggcgggtcg cggccagcta cgccgagctg ctcaccccgg ccccgttcac cctgaccagc
     2221 ttccccaacg acgagggcta cgacgagctg gtgctggcca gggccatccc gttctcatcg
     2281 ctgtgccagc accacctgct gcccttcacc ggggtcgccc acgtcggcta cctaccggcc
     2341 gggcggatcc tggggctgtc caagctggcc cgggtggtcg agctgttcgc ccgccgcctg
     2401 cagctccagg agcggctcac cacccaggtc gccgactggc tccagaccca gctcgagccc
     2461 aagggcgtcg gggtggtgct ggaggccgag cacctgtgca tgaccctgcg cggcgtccag
     2521 gcaaccggcg cccgcaccgt gacctcggcc ctgcacggcc ggctgcgcga cgacccgcgc
     2581 tcgcggcagg agttcctcgc cctcacccgc accgccacct gaaccacccg cccaccacca
     2641 ggagttccca tgaccgccac ccccacgtcc acatttgtca tcgtcggtgc cggcctggcc
     2701 ggcgccaagg cggccgagac cctgcgcgcc gagggattcg acggccgcct gctgctgctc
     2761 ggcgaggagc ccgagcggcc ctacgagcgc ccgccgctgt ccaaggccta cctgcgcggc
     2821 gaggccgacc gcgacagcct ctatgtgcac caggaaggct tctacgccgc ccacgacatc
     2881 gagctgcgcc cctcgacccc ggtccgctcg atcgacccgg gcggccgcca gctcgagctc
     2941 gcctcgggcg agcggatcgg ctatgagcgg ctgctgctgg ccaccggcgc caccccgcgt
     3001 cagctgcccc tgcccggggc ggacctggcc ggggtccagt atctgcgcac tcgccaggac
     3061 gccgacgtgc tcgcggccgc cgcggcgcgt gccgagcagg tcgtggtggt ggggacgggc
     3121 tggatcggc
//
LOCUS       DASSWR010000099         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_541058,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000099 DASSWR010000000
VERSION     DASSWR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541058"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1102
                     /locus_tag="VFO47_01605"
     CDS             197..1102
                     /locus_tag="VFO47_01605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8787428.1"
                     /translation="MPATLEVGHRLGPFRIEERLGQGGMGTVFRAVHDDGRVVALKVL
                     RDELGADQSFRTRLAREARAAAEVDHPNLARVLEAGEADGRLYLAVRYVDGRSLAERL
                     AAGGPLQVPELVRLAADAGAGLDALHRRGIVHRDVKPANLLLAADGTAVVGDFGLAKN
                     RAWTVLTRPGQVLGTLDYLAPELIRGEPADPMSDLYALGCVLYECLAGAPPFAGRGVL
                     RIGMAHLEEEPGDPAAGRADVPPALSWTIRQALAKDPSRRPPAAGTLARMLRLAAADS
                     RPTGSVRPAGDGLEVEDHGSRNAPG"
     gene            complement(1200..1649)
                     /locus_tag="VFO47_01610"
     CDS             complement(1200..1649)
                     /locus_tag="VFO47_01610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8787429.1"
                     /translation="MAIDTRAVTAVGRPREEVAAYLRDPANDPTWIGGLRSARLVTDP
                     PVGVGSRVARVASFLGRRIEYVNEITELTGTRLAMRSVRSPFPMRVTYGYDDAGEGGT
                     EVSVRVEGDASRLYRLADPLLAALVRRSVRRDLRTLKRVLEDGAGGR"
     gene            complement(1625..2101)
                     /locus_tag="VFO47_01615"
     CDS             complement(1625..2101)
                     /locus_tag="VFO47_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET8787430.1"
                     /translation="MTDPEAVTREQVVPAPPGEVFRWFVDPALLARWIGVRATLEPRP
                     GGVFRFEITDGEWCSGRYLEVVPGRRVVFTWGWESGAIPVPPGSSTVEVELAPHPGGT
                     LVRLVHRDLAPEVRPLPDDGWSRFLPRLAAVVAGQEPLPYPTEPPEEVPWPSTPGP"
     gene            complement(2098..2406)
                     /locus_tag="VFO47_01620"
     CDS             complement(2098..2406)
                     /locus_tag="VFO47_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8787431.1"
                     /translation="MPVDDTLRALAHPARRAMLALAWDAERSSSELAEAVALTRPAAS
                     QHLKVLRDAGLVHVRVDGNQRLYRVDPTRLEEVRRQLEGFWGDRLGRLAAGLDGEEER
                     "
     gene            complement(2473..3237)
                     /locus_tag="VFO47_01625"
     CDS             complement(2473..3237)
                     /locus_tag="VFO47_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8787432.1"
                     /translation="MTARRRGGQVEALPRALSYARPKGEQLQEILEGVIDELQPGALL
                     PSERLLAERYGVARATVTQAVEGLASRGLVYRVHGSGTFVAEPKFRQPLTLTSFTEDM
                     RARGMTPGSIVRSQAVVPASEVVARHLAMVPGSPVVHLERVRTADGEPMALERTHLPA
                     QRLPGLEAANLADASLYELLERRWGVRVADADQWASVVRLTEDEAALLQVAPDQPALL
                     FQRLTRDPSGAPIEYVRSLYRGDRYEVHTHLERLGG"
     gene            3259..>3441
                     /locus_tag="VFO47_01630"
     CDS             3259..>3441
                     /locus_tag="VFO47_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787433.1"
                     /translation="MTTGLGHFTVHRKARERHGRDGDARPLPRAGREAAVTARLLVTG
                     ARVLTPGGEWPRGWIAV"
BASE COUNT          441 a         1291 c         1323 g          386 t
ORIGIN      
        1 ggcccgcagg accacgacgg cgaccacggc caccagcgtc agcagcagca cgtaggccgc
       61 cacgagccgg cggcccgacc ggtcggcggt gcccgaattg ccgcccatgg cggctatcat
      121 aggcggcgcg tgcggaaccc ccgcctcctc cggggaccag gcttgagccc cggacccaga
      181 cctgcaggga tgccgaatgc ctgccacgct cgaggtcggc caccggctcg ggcccttccg
      241 gatcgaggag cggctgggcc agggcggcat ggggacggtg ttccgcgcgg tccacgacga
      301 cggccgcgtg gtcgccctca aggtgctgcg cgacgagctc ggcgccgacc agtccttccg
      361 cacccggctg gcccgcgagg ccagggccgc ggccgaggtc gaccacccca acctggcccg
      421 ggtcctggag gcgggggagg cggacggccg cctctacctg gccgtgcgct acgtcgacgg
      481 ccgctcgctg gccgagcgcc tggccgccgg cgggccgctg caggtccccg agctggtgcg
      541 gctggccgcc gacgccgggg ccggcctgga cgcgctgcat cggcggggga tcgtgcaccg
      601 cgacgtcaag ccggccaacc tgctgctcgc cgccgacggg acggccgtgg tcggcgactt
      661 cgggctggcc aagaaccgcg cctggacggt gctgacccgg cccggccagg tcctcgggac
      721 cctcgactac ctggccccgg agctgatccg gggcgagccg gccgacccga tgagcgacct
      781 gtacgcgctc ggctgcgtcc tctacgagtg cctcgccggg gcgccgccgt tcgccggccg
      841 gggggtcctg cggatcggca tggcccacct ggaggaggag ccgggcgacc cggccgccgg
      901 gcgggccgac gtcccgccgg ccctgtcctg gacgatccgc caggcgctgg ccaaggaccc
      961 gtcccggcgg ccgcccgcgg cgggcacgct ggcccgaatg ctccgcctgg ccgccgccga
     1021 cagccggccc accggaagcg tgcgcccggc cggcgacggc ctcgaggtcg aggaccacgg
     1081 gtcacgcaac gcacctgggt gaacggcgcc cgggtcgacg gccccgccgg cctcgccccg
     1141 gggtggcgac ccgtcgcctc accccgaccc cgctcgttcg gcacggtgat cgcgacccgc
     1201 tagcgacccc cggcgccgtc ctccagcacc cgcttgaggg tgcgcaggtc gcgccggacc
     1261 gatcgccgca ccagcgcggc caggagcggg tcggcgagcc ggtagaggcg gctggcgtcg
     1321 ccctcgaccc ggaccgagac ctcggtcccg ccctcacccg cgtcgtcgta gccgtaggtg
     1381 acccgcatgg ggaagggcga ccggaccgag cgcatggcca gccgggtccc ggtcagctcg
     1441 gtgatctcgt tgacgtactc gatgcggcgg cccaggaacg aggcgacccg cgcgacccgg
     1501 ctgcccacgc cgaccggcgg gtcggtgacc agccgggccg agcgcagccc gccgatccag
     1561 gtcgggtcgt tggccgggtc gcgcaggtag gcggcgacct cctcgcgggg ccggccgacg
     1621 gcggtcacgg cccgggtgtc gatggccacg ggacctcctc gggcggctcg gtggggtagg
     1681 ggagcggctc ctggccggcg acgaccgcgg ccagacgggg caggaaccgg gaccagccgt
     1741 cgtcggggag ggggcggacc tcgggggcga ggtcgcggtg gacgaggcgg accagggtgc
     1801 cgccgggatg cggcgccaac tcgacctcga cggtggagga gccgggcggc accgggatgg
     1861 cgccgctctc ccagccccag gtgaacacca cccgccggcc gggcacgacc tccaggtagc
     1921 ggcccgagca ccactcgccg tcggtgatct cgaaccggaa gacgcccccg ggccgcggct
     1981 ccagggtcgc ccggacgccg atccagcggg ccagcagggc cgggtcgacg aaccagcgga
     2041 acacctctcc gggcggggcg ggcacgacct gctcgcgggt cacggcctcc gggtcggtca
     2101 ccgctcctcc tcgccatcaa gcccggcggc cagccggccc agccggtcgc cccagaaccc
     2161 ctcgagctgg cggcgcacct cctccagccg ggtcgggtcg acccggtaca gccgctggtt
     2221 gccgtccacc cgcacgtgga ccagcccggc gtcgcgcagc accttgaggt gctgggaggc
     2281 ggcgggccgg gtcagcgcca ccgcctcggc cagctccgac gacgaccgct cggcgtccca
     2341 ggccagcgcc agcatggccc gccgcgcggg atgggcgagc gcccgcaggg tgtcgtcgac
     2401 cggcattgat taagcataag cgcaattaag tagccatgca atcagtggag gggtggggcg
     2461 gagccggccg gatcagccgc ccaggcgctc caggtgggtg tggacctcgt agcggtcgcc
     2521 gcggtagagg gagcggacgt attcgatcgg ggcgccgctg gggtcgcggg tgaggcgctg
     2581 gaacagcagg gccggctggt cgggggcgac ctggaggagg gccgcctcgt cctcggtgag
     2641 gcggaccacg ctggcccact ggtcggcgtc ggccacccgc accccccagc ggcgctccag
     2701 cagctcgtac agggaggcgt cggccaggtt ggccgcctcc agcccgggca gccgctgggc
     2761 cgggaggtgg gtgcgctcca gggccatcgg ctcgccgtcg gcggtccgga cccgctccag
     2821 gtggaccacc gggctgcccg ggaccatggc caggtggcgg gccacgacct cgctggccgg
     2881 caccaccgcc tggctgcgca cgatcgagcc cggagtcatg ccccgggccc gcatgtcctc
     2941 ggtgaaggag gtcagggtga gcggctggcg gaacttgggc tcggccacga aggtgccgct
     3001 gccgtggacc cggtagacca gcccgcggga ggccagcccc tcgaccgcct gggtgacggt
     3061 cgccctggcc actccgtagc gctcggccag cagccgctcg gaggggagca gggctcccgg
     3121 ctggagctcg tcgatcaccc cctccaggat ctcctggagc tgctcgccct tgggccgggc
     3181 gtaggagagc gcccggggga gcgcctcgac ctggcctccg cgtcgccgtg ccgtcatgcc
     3241 gacaagtcta ccgccctctt gacaactgga ctaggccact ttaccgtcca ccgcaaggca
     3301 agggaacgcc atggaaggga cggcgatgct cgaccgctac ctcgagcggg ccgggaggcg
     3361 gccgtgacgg cccggctgct ggtcaccggg gcccgggtgc tgaccccggg cggggagtgg
     3421 ccgcggggct ggatcgccgt g
//
LOCUS       DASSWR010000100         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_2625,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000100 DASSWR010000000
VERSION     DASSWR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2625"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VFO47_01635"
     CDS             complement(<1..171)
                     /locus_tag="VFO47_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase assembly protein HupF"
                     /protein_id="HET8787434.1"
                     /translation="MSDPVECHGDVCITCSDEAVQVRVVRLLDADLAVVDTGQSMEEV
                     SVALVDATVGDTI"
     gene            complement(168..794)
                     /locus_tag="VFO47_01640"
     CDS             complement(168..794)
                     /locus_tag="VFO47_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET8787435.1"
                     /translation="MRQRLSETLQAAATDALEQRAKPVEALADDAEQVAAACHAMAVR
                     FHRGGKLVCFGSGGPSTDAQHIAVEFVHPVIVGKRALPAISLTGDVATLTSIAGREGY
                     AEVYAHQLRHLAAPEDIALGVSVDGNDPAVLAGLEAAAELGMLTIALVGGDGGQTAQS
                     GAVHHALVARSDDPLVVKEVHVTAYHVLWELVHVLFEQPGVLDPEVIR"
     gene            972..1526
                     /locus_tag="VFO47_01645"
     CDS             972..1526
                     /locus_tag="VFO47_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787436.1"
                     /translation="MEERAGDARGDRVPDGAPVAVPHETVVVDPVFVVTQTFPARPSS
                     IPDIRDFVLRYLRDAPLTPEGSQQLHHAVLNALLEAASPDGGQIQVCFRIFPDGVEID
                     VLRGPASAGSTFPPVRRDRPEHRSFAEWLAGVLKREGLSQEAAARQLGVSVKTVSRWV
                     GGETEPRLRELRRIHEVFGEAPLG"
     gene            complement(1636..2397)
                     /locus_tag="VFO47_01650"
     CDS             complement(1636..2397)
                     /locus_tag="VFO47_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787437.1"
                     /translation="MTTYVLVAGAWLGGWAWQPATDQLRRHGHDVHPVTLTGLGDRSD
                     LASPQVDLDTYISDVVDLVEREELHDVVLVGHSYAGHVVTGVADRIPERISLLAYLDA
                     GPSPDGTAFMDLQPPAARELIERLVDEAGDGWRIPMPSWEELEGTMGASLEGLGQDER
                     DHMRAHATAQPLRTWTQPLSLKSPARQDLPKLLITCSLPLAQVREMIAAGHPWFAALA
                     GPQWSFRELPTGHWPMFSVPDKLASLLLDLSSGSR"
     gene            2459..3136
                     /locus_tag="VFO47_01655"
     CDS             2459..3136
                     /locus_tag="VFO47_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET8787438.1"
                     /translation="MSTPLEDPRPRGAGQRAGLTRAAVLRAARRIADEEGVERLTMRR
                     LAAELGVMPNALYTYVPGKEGLLDALIDDLLAGVDTGEVAADWRDGLVRVMDSSRRLL
                     LAHPRLVPVFLARPGLGPNAARLGEITFALLRRGGIQGERAVEAFRVLLIYSLGFAAF
                     QATRLPDDPARSARAEAVFASLPEDEFPEMHRLARDLAGPTTDRQFATGLRWLLDGIA
                     AQAAQSR"
     gene            complement(3124..>4125)
                     /locus_tag="VFO47_01660"
     CDS             complement(3124..>4125)
                     /locus_tag="VFO47_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787439.1"
                     /translation="DLVAVNGRLARVAALGGTAGALLGVGLDRLLGGGAVLWAAAATF
                     AAGGLTALSLPAARSRRDRAAGSLDRAAGSLDRESGSLDRGSGSLDPGSGPLDPGSRP
                     VDRGPSPGGSGTRPYRDPRSRPLDPGPRPRRGSMDRRVARLARPPGRVRLARTANTCV
                     RAVGGFTLFLLAFELRRQGVGTVGLGLLLAGVGVGGVIGAFLLPRAARLVREDGLLAG
                     GLLLCGTTAWLLAGRVGVETAAAGGLAMGAGIAAARLGFESLVQREVPPQARGTAITR
                     AETTFQLAWVTGAVLPVAFPLPTTPSLVAAALACLFAATTYTVGLLRLRRGRDRQRL"
BASE COUNT          543 a         1500 c         1569 g          513 t
ORIGIN      
        1 gatggtgtcc ccgacggtcg cgtcgaccag ggccacgctg acctcctcca tgctctggcc
       61 ggtgtcgacc acggccaggt cggcgtcgag cagccgcacc acccgcacct ggaccgcctc
      121 gtccgagcag gtgatgcaga cgtcgccgtg acattcgacg gggtcgctca acggatcacc
      181 tccgggtcga gcacgcccgg ctgctcgaac agcacgtgca ccagctccca caggacgtgg
      241 taggcggtga cgtggacctc cttgacgacc agcgggtcgt cggaccgggc gaccagggcg
      301 tggtgcacgg ccccgctctg ggccgtctgg ccgccgtcgc ccccgaccag ggcgatggtg
      361 agcatcccga gctcggccgc ggcctccagc ccggccagga ccgccgggtc gttgccgtcg
      421 accgacaccc cgagggcgat gtcctcgggg gcggccaggt ggcggagctg gtgggcgtag
      481 acctcggcgt agccctcgcg gccggcgatg ctggtcaggg tggccacgtc gccggtcagc
      541 gagatcgccg ggagggcccg cttgcccacg atcaccgggt ggacgaactc gacggcgatg
      601 tgctgggcgt cggtgctggg gccgccgctg ccgaagcaga cgagcttgcc gccacggtgg
      661 aagcggacgg ccatcgcgtg gcaggcggcc gccacctgct cggcgtcgtc ggccagggcc
      721 tcgaccggtt tggcccgctg ctcgagggca tcggtggccg ccgcttggag cgtctccgac
      781 agtcgctgtc gcatgcacgg ccctcccggc agcctaccgg catctgggaa gcatgcgccg
      841 tcgctgcccg ctttgcaaga cacttctgtc cggacagctt tcggacagag cgtcacgttt
      901 gcctgattcc tgggaccgga gcgcaacctc gcctacaatc gcacaaattg gcggcggcga
      961 gggagcgatc cttggaggag cgcgctggtg acgcccgggg ggaccgggtg cccgacggcg
     1021 ctccggtggc ggtgccgcac gagacggtcg tcgtcgaccc tgtcttcgtg gtcacccaga
     1081 ccttcccggc ccggccgtcg tccatccccg acatccgcga cttcgtgctg cgctatctgc
     1141 gcgacgcccc gctgaccccc gaaggcagcc agcagctcca ccacgccgtg ctgaacgccc
     1201 tgctggaggc ggccagcccc gacggcggcc agatccaggt ctgcttccgc atcttccccg
     1261 acggggtcga gatcgacgtc ctgcgcggcc cggccagcgc cggcagcacc ttcccaccgg
     1321 tccgccgcga ccggcccgag caccgctcgt tcgccgagtg gctggcgggc gtgctcaagc
     1381 gcgagggcct ctcccaggag gccgcggccc gccagctcgg cgtgtcggtg aagacggtca
     1441 gccgctgggt cggcggcgag accgagcccc gcctgcgcga gctccgccgc atccacgagg
     1501 tcttcggcga ggccccgctg ggctagcccg tggtcgatgt gcccaccgcc gccatggtgg
     1561 tcaccctggc ctggcaccga ggtcagttgg ccctcacccc tgactccagc gccaggtgac
     1621 tgcatggggg tggcgtcagc gcgacccgga cgagaggtcg agcagcaggg aggcgagctt
     1681 gtcggggacc gagaacatgg gccagtgccc ggtggggagc tcccggaacg accactgggg
     1741 gccggcgagg gcggcgaacc aggggtggcc ggcggcgatc atctcgcgca cctgggccag
     1801 gggcaggctg caggtgatca gcagcttggg caggtcctgg cgggcggggc tcttcaggga
     1861 gaggggctgg gtccaggtgc ggagcggctg ggcggtggcg tgggcgcgca tgtggtcgcg
     1921 ctcgtcctgg ccgagtccct ccaggctggc gcccatggtg ccctccagct cctcccagga
     1981 gggcatgggg atccgccagc catcgccggc ctcgtcgacc aggcgctcga tcagctccct
     2041 ggcggcgggc ggctggaggt ccatgaaggc ggtcccgtcg gggctcggcc cggcgtcgag
     2101 gtaggcgagc agggagatgc gctcggggat ccggtcggcc actcccgtga ccacgtgccc
     2161 ggcgtagctg tgcccgacca ggacgacatc gtggagctcc tcgcgctcga ccaggtcgac
     2221 gacgtcggag atgtaggtgt cgaggtcgac ctgggggctg gccaggtccg agcggtcccc
     2281 cagcccggtg agcgtcaccg ggtggacgtc gtgcccgtgg cggcggagct ggtcggtggc
     2341 cggctgccag gcccatccgc ccagccaggc cccggcgacg aggacgtagg tggtcatccg
     2401 cgcgatccct cctctgagtt gtacgttgta caattactgt acgttgtaca atagcgctat
     2461 gtcaaccccc ctcgaggacc cacgaccccg cggcgccggc cagcgtgccg gcctgacccg
     2521 ggcggcggtg ctgcgggccg cccgccgcat cgccgacgag gagggtgtgg agcggctcac
     2581 catgcgccgc ctggccgccg agctgggcgt gatgccgaac gccctctaca cctacgtccc
     2641 tggcaaggag gggctgctcg acgccctgat cgacgacctg ctcgcgggcg tcgacaccgg
     2701 cgaggtggcc gccgactggc gggacggcct ggtccgggtg atggactcct cgcgccggct
     2761 gctgctggcc catccgcggc tggtgccggt gttcctcgcc cgtcccgggc tcggccccaa
     2821 cgcggcccgg ctcggcgaga tcaccttcgc gctgctgcgg cggggtggca tccagggcga
     2881 gcgggcggtc gaggcgttcc gggtcctgct gatctactcg ctcgggttcg ccgccttcca
     2941 ggcgacccgg ctgccggacg atcccgcccg ctcggcccgg gccgaggccg tgttcgccag
     3001 cctgccggag gacgagttcc ccgagatgca ccggctggca cgggacctcg ccgggcccac
     3061 caccgaccgg cagttcgcca ccggcctgcg ctggctgctc gacgggatcg ccgcccaggc
     3121 cgctcagagc cgctgacggt cgcgaccccg gcggaggcgc agcaggccga cggtgtaggt
     3181 ggtggcggcg aacaggcagg cgagcgcggc cgcgaccagg gacggggtcg tgggcagggg
     3241 gaaggcgacg gggaggacgg cgccggtcac ccaggcgagc tggaaggtgg tctcggcgcg
     3301 ggtgatggcg gtgccgcggg cctgcggggg aacctcgcgc tggacgaggc tctcgaagcc
     3361 gaggcgggcg gcggcgatgc cggcgcccat ggcgaggccg ccggcggcgg cggtctcgac
     3421 cccgacccgg ccggccagga gccaggcggt ggtgccgcac aggagcaggc cgccggcgag
     3481 gaggccgtcc tcccggacca ggcgggccgc ccgcggaagg aggaaggcgc cgatcacccc
     3541 gcccaccccg accccggcca ggagcaggcc gagaccgacg gtgccgacgc cctggcgtcg
     3601 cagctcgaag gcgagcagga acagggtgaa cccgccgacc gcccggacgc aggtgttggc
     3661 cgtcctggcc agccgcaccc gccccggtgg ccgggccagc cgggccaccc gccggtccat
     3721 cgacccccgc cgggggcgag gccccgggtc gagcgggcgg gaacgcgggt cgcggtacgg
     3781 gcgggtgccg gagccaccgg gagagggacc ccggtcgaca ggacgggaac ccgggtccag
     3841 cggcccagac cctggatcca gcgacccgga cccccggtcc agggacccgg actcccgatc
     3901 cagcgacccc gcagcccggt ccagcgaccc cgcagcccga tcccggcggg agcgggccgc
     3961 cggaagcgac agggcggtca gcccgccggc ggcgaaggtg gcggcggcgg cccacagaac
     4021 cgcgccgccg cccaggaggc ggtcgaggcc gaccccgagc agggccccgg cggtcccgcc
     4081 cagggcggcc accctggcca ggcggccgtt cacagcgacc aggtc
//
LOCUS       DASSWR010000101         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1332386, whole genome shotgun sequence.
ACCESSION   DASSWR010000101 DASSWR010000000
VERSION     DASSWR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332386"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VFO47_01665"
     CDS             complement(<1..1580)
                     /locus_tag="VFO47_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HET8787440.1"
                     /translation="MPPDPDVVLRRLTDRLAPLERDLHRAFWAASTDARPETSATRQR
                     AEEAWLATLGDPELFAEVQGALAAAGAAGTGPYARRALEQANLDLLANQIPEGDRAEL
                     VTLQAKVEHAFSTVRGRVGGRELANNELERILTDSTDPAERRAAWEAGKQVGAAVADD
                     VLALVELRNRVARERGYRDWFAFALAVGDIDEAWLERMLDEVESATREPFLAAKAALD
                     ARQSARFGVAVEDLYPWHYGDLFFQRYNGDGEADLGPLLEGTDTVKLTVTTYDGMGLE
                     TRHLLERSDLHPRPGKDQHAFCLDVDRAGDIRVLANLAPGEELGVAPEPAQHQGHGLG
                     DQRLGRAAEHPGQRPVDVVVVDGVADLVQQHVQPLLAGGQVGQHPDVAGPVDVQAEGM
                     LVLAGAGVEVGPLQQVAGLEAHAVVGGDGEPDGVGALQQRAQVGAGLAVVALEEQVAV
                     VPGPQGVDVDPEAGAQAGVERGLGGQERLPGGRLDLLQDPLQPGLVDVAGGQGEGEPV
                     AVAALASDPVAQLDQGQHV"
     gene            1629..1850
                     /locus_tag="VFO47_01670"
     CDS             1629..1850
                     /locus_tag="VFO47_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787441.1"
                     /translation="MRYRYVVRIGPEDVGQRVVVRWRRPAPGGDEVADVVGPLEAADE
                     RRFAVRNRRGELVEIPRDRALAAKVIPPR"
     gene            complement(1834..>2169)
                     /locus_tag="VFO47_01675"
     CDS             complement(1834..>2169)
                     /locus_tag="VFO47_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787442.1"
                     /translation="GRDGTMAGVRDMALHDGELHVVTGNIDAREKGSVLLEDYPEGAH
                     TMATHWRCAFPGDRRAGHLVPERVREFPDLSAIEGIAVDHQDRTFYVSDEDEGVVVKH
                     THVLVSEEG"
BASE COUNT          250 a          789 c          828 g          302 t
ORIGIN      
        1 acgtgctggc cctggtcgag ctgcgcaacc gggtcgctcg cgagcgcggc taccgcgact
       61 ggttcgcctt cgccctggcc gccggcgaca tcgacgaggc ctggctggag cggatcctgg
      121 aggaggtcga gtcggccacc cgggagccgt tcctggccgc caaggccgcg ctcgacgccc
      181 gcctgagcgc ccgcttcggg gtcgacgtcg actccctgcg gccctggcac tacggcgacc
      241 tgttcttcca gcgctacgac ggcgaggccg gcgccgacct gggcccgctg ctggagggcg
      301 ccgacaccgt cgggctcacc gtcgccacct acgacggcat gggcctcgag acccgccacc
      361 tgctggagcg gtccgacctc cacccccgcc ccggcaagga ccagcatgcc ttctgcctgg
      421 acgtcgaccg ggccggcgac atccgggtgc tggccaacct ggcccccggc gaggagtggc
      481 tggacgtgct gctgcacgag gtcggccacg ccgtctacga cgaccacatc gaccggtcgc
      541 tgccctgggt gctccgccgc ccgccccagc cgctggtcac cgaggccgtg gccctgatgc
      601 tgggccggct ccggcgcgac cccgagttcc tcgccggggg ccaggttggc caggacccgg
      661 atgtcgccgg cccggtcgac gtccaggcag aaggcgtgct ggtccttgcc cgggcggggg
      721 tggaggtcgg accgctccag caggtggcgg gtctccaggc ccatgccgtc gtaggtggtc
      781 acggtgagct tgaccgtgtc ggtcccctcc agcagcgggc ccaggtcggc ctcgccatcg
      841 ccgttgtagc gctggaagaa caggtcgccg tagtgccatg ggtacaggtc ctcgacggcg
      901 accccgaagc gggcgctctg gcgggcgtcg agggcggcct tggcggccag gaagggctcg
      961 cgggtggccg actcgacctc gtccagcatc cgctccagcc aggcctcgtc gatgtcgccg
     1021 acggccaggg cgaaggcgaa ccagtcgcgg tagccgcgct cgcgggccac ccggttgcgc
     1081 agctcgacca gggcgaggac gtcgtcggcg acggcggcgc cgacctgctt gccggcctcc
     1141 caggcggcgc gccgctcggc cgggtcggtc gagtcggtca ggatccgctc gagttcgttg
     1201 ttggccagct cccggccgcc gacccggccg cggacggtcg agaaggcgtg ctccaccttg
     1261 gcctgcaggg tcaccagctc ggcccggtcg ccctcgggga tctggttggc cagcaggtcg
     1321 aggttggcct gctccagggc gcggcgggca tacgggccgg tcccggccgc cccggcggcg
     1381 gccagggcgc cctggacctc ggcgaacagc tccgggtcac cgagggtggc cagccaggcc
     1441 tcctcggccc gctgccgggt cgccgaggtc tcgggccggg cgtcggtcga ggccgcccag
     1501 aaggcgcggt ggaggtcgcg ctccagcggg gccaggcggt cggtgagccg ccgcaggacg
     1561 acgtcggggt cggggggcat ggcacgggct ccgggcgctg gacggtcggt cgcccctact
     1621 gtagtgggat gcgctaccgg tacgtggtcc ggatcgggcc ggaggacgtc ggccagcggg
     1681 tggtggtccg ctggcgccgt cccgcgcccg gcggcgacga ggtggccgac gtggtcggtc
     1741 ccctggaggc cgccgacgag cggcgcttcg cggtccgcaa ccgccgcggc gagctggtcg
     1801 agatcccccg cgaccgcgcg ctggccgcca aggtcatccc tcctcgctga ccaacacgtg
     1861 ggtgtgcttg acgaccacgc cctcatcctc gtcgctgacg tagaaggtgc ggtcctggtg
     1921 gtcgacggcg atgccctcga tggccgacag gtcggggaac tcgcggaccc gctcggggac
     1981 caggtggccg gcgcggcggt cgccggggaa cgcgcagcgc cagtgggtgg ccatggtgtg
     2041 ggcgccctcg gggtagtcct ccaacagcac actgcccttc tcgcgggcgt cgatgttgcc
     2101 ggtgaccacg tgcagctcgc cgtcgtgcag ggccatgtcg cggaccccgg ccatggtccc
     2161 gtcgcggcc
//
LOCUS       DASSWR010000102         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_456245,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000102 DASSWR010000000
VERSION     DASSWR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456245"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFO47_01680"
     CDS             complement(<1..181)
                     /locus_tag="VFO47_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787443.1"
                     /translation="MQESWGTFRDRVSRLGWEQASDRPMAWRAPWRHLDPTVLLAALA
                     LTALGLLAIYSSTFAG"
     gene            complement(181..2142)
                     /gene="mrdA"
                     /locus_tag="VFO47_01685"
     CDS             complement(181..2142)
                     /gene="mrdA"
                     /locus_tag="VFO47_01685"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET8787444.1"
                     /translation="MHPPSSPFRSRLTVLSILVAVLFVALSSRLWYLQVLAGERYGDL
                     AEGNRVRQVVVEAPRGRILDREGRVVVRNRAAWAVTVKPSELGDRREVLGRLARLLGT
                     SRARLEDRIAGYTGSPLRGIPVAEDVPTRQLFHLTEHADRFPGVAPEVLALREYPAGP
                     AAAHVLGYVGEISAGELKERRFRGYKAGDIVGKAGVELTYDKWLRGRDGVQDLEVNAA
                     GEVVRVLGGRPPVPGRDLQLNLDLNLQVSVERALADGMRAARRLPDSQRGGTYPAPAA
                     TAVVLDPDDGAVAALASLPQYDPRRFVGGISRRDFARYAGSPGKPLLHRAVQSAYPPG
                     STWKPFTALAGLEAGTITPTSTYHCPGSYTIGSYTKRDWTPRGHGTVGLGDSLRLSCD
                     VYYYSLGAQLGVAERAQEERGQRVDEQMQATARAFGFGRRPPLDLPYGAEGTVPTREW
                     RKRFWEENRKTYCAGSSALYRELCRDGWRWQGGDNLNIAIGQGDLQVSPLQLAVGYGA
                     LANGGTVYRPHVGRAVLDPASGRVVRRVAPRVTGVAEVPGGALAAVGQGLASVTTGGT
                     AASAFAGFPLDRFPVAGKTGTADLPPQAPFAWFASYAPVGNPEYVVVVMVEQGGHGGE
                     TAAPVARAIYQKLFGLPVTPVAPGADRSG"
     gene            complement(2152..2652)
                     /gene="mreD"
                     /locus_tag="VFO47_01690"
     CDS             complement(2152..2652)
                     /gene="mreD"
                     /locus_tag="VFO47_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET8787445.1"
                     /translation="MRRPLVLTAVLVVAVLVQSTVLAELRLAGVRPDLLVLAVVAVAM
                     ASDATTGAVFGFVTGLVADLLFDLPVGVSALVYTAAGFAVGTVRVYVTSHRPLVHLVL
                     AGAASLASVWCCGLLLRVFDLSSWAVVARAGPLVAVYNLLLTPFVYPVVWALTERVPA
                     RPARRW"
     gene            complement(2656..3501)
                     /gene="mreC"
                     /locus_tag="VFO47_01695"
     CDS             complement(2656..3501)
                     /gene="mreC"
                     /locus_tag="VFO47_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET8787446.1"
                     /translation="MSRSPRRARTVLAVLLAAAIVLITIDFRQPDGGPVDRLQRLAIA
                     VFGPLQRGASAVVRPVADLAGGVSELGGLREDNRRLEAEVARLRAQERTYADLLGENR
                     RLRGALRMAARCGCRTVGASVVASSGSNFQLSVTVDAGSRQGVAKDMAVVDADGLVGR
                     VTQVTADYATVLLVTDPASGVAATLARAKAPGIVKGSGAQLLSFQPVRAGTAVRRGDP
                     VVTQGYQGGVFPAGLPVGVVERVDPAGAASLVPRVAVRPYAALGALDVVAIVVERPAP
                     PVRGS"
BASE COUNT          488 a         1343 c         1382 g          375 t
ORIGIN      
        1 gcccggcgaa ggtcgaggag tagatggcga gcaggcccag ggcggtcagg gccagcgcgg
       61 ccagcagcac cgtcgggtcc aggtgccgcc agggcgcccg ccaggccatc ggccggtcgc
      121 tggcctgctc ccagccgagc cgcgacaccc ggtcgcggaa ggttccccac gactcctgca
      181 tcagccgctc cgatccgccc cgggggccac cggggtcacc ggcaggccga acagcttctg
      241 gtagatggcc ctggccaccg gggcggccgt ctcgccgccg tggccgccct gctcgaccat
      301 gaccacgacc acgtactccg ggttccccac cggcgcgtag gacgcgaacc aggcgaacgg
      361 ggcctggggc ggcaggtcgg cggtgccggt cttgccggcc accgggaacc ggtccagcgg
      421 gaacccggcg aatgcggagg ccgccgtccc gcccgtggtc acgctggcca gcccctggcc
      481 gacggcggcc agggcgccgc cgggcacctc ggccaccccg gtgacccggg gcgcgacccg
      541 ccggacgacc cgcccgctgg ccgggtcgag caccgcccgg ccgacgtggg gccggtagac
      601 ggtgccgccg ttggccagcg ccccatagcc gacggcgagc tggagcggcg acacctggag
      661 gtcgccctgg ccgatggcga tgttgaggtt gtcgcccccc tgccagcgcc agccgtcgcg
      721 gcacagctcc cggtacaggg cgctggaccc ggcgcagtag gtcttgcggt tctcctccca
      781 gaagcgcttg cgccactccc gggtcggcac cgtgccctcg gccccgtacg gcaggtcgag
      841 ggggggccgc cgcccgaacc cgaacgcccg ggcggtggcc tgcatctgct cgtcgacccg
      901 ctggccgcgc tcctcctggg cccgctcggc caccccgagc tgagcgccca ggctgtagta
      961 gtagacgtcg caggacagcc gcaggctgtc gccgagcccg accgtgccgt ggccccgcgg
     1021 ggtccagtcc cgcttggtgt agctgccgat ggtgtacgac ccagggcagt ggtaggtgct
     1081 ggtcggggtg atggtgccgg cctccaggcc ggccagggcg gtgaacggct tccaggtcga
     1141 gccgggcgga taggccgact ggacggcccg gtgcagcagc ggcttgcccg ggctgccggc
     1201 gtagcgggcg aagtcgcgcc gggagatgcc gccgacgaag cgccgcgggt cgtactgggg
     1261 caggctggcc agggcggcca cggcgccgtc gtcgggatcg agcacgaccg cggtcgcggc
     1321 cggggccggg taggtgccgc cgcgctggct gtcgggcagc cggcgggcgg cgcgcatgcc
     1381 gtcggccagg gcccgctcga cactcacctg gaggttgagg tcgaggttga gctggaggtc
     1441 gcggccgggg acgggcgggc ggccgccgag gacccggacg acctcgccgg cggcgttgac
     1501 ctcgaggtcc tggacgccgt cgcggccccg cagccacttg tcgtaggtca gctcgacccc
     1561 ggccttgccg acgatgtcgc cggccttgta cccgcggaag cgccgctcct tgagctcgcc
     1621 ggccgagatc tcgccaacgt agccgagcac gtgggcggcg gccgggccgg ccgggtactc
     1681 gcgcagggcc aacacctcgg gggcgacccc ggggaaccgg tcggcgtgct cggtcaggtg
     1741 gaagagctgg cgggtgggga cgtcctcggc caccgggatg ccgcgcaggg gcgagccggt
     1801 gtagccggcg atgcggtcct ccagccgggc cctgctggtg ccgagcagcc gggccaggcg
     1861 gccaaggacc tcgcggcggt cgcccagctc cgacggcttg accgtgaccg cccaggcggc
     1921 ccggttgcgg accaccaccc ggccctcgcg gtccaggatc cggccccggg gcgcctcgac
     1981 cacgacctgg cggacccggt tgccctcggc caggtcgccg tagcgctcgc cggccagcac
     2041 ctggaggtac cacagccgcg acgacagggc cacgaacagc accgccacca ggatggacag
     2101 gacggtgagg cgggagcgga acggggagga gggcgggtgc acggcgcgaa cctaccaacg
     2161 ccgggccggg cgggcgggga cgcgctcggt cagcgcccac accaccgggt agacgaacgg
     2221 ggtcagcagc aggttgtaga cggcgaccag ggggccggcc cgggccacga ccgcccacga
     2281 cgacaggtcg aacacccgca gcagcagccc gcagcaccac accgaggcca ggctggccgc
     2341 gccggccagg accaggtgga cgagcgggcg gtggctggtc acgtacaccc ggacggtccc
     2401 cacggcgaac ccggcggcgg tgtagaccag cgccgacacc ccgaccggca ggtcgaacag
     2461 gaggtcggcg accagcccgg tgacgaagcc gaacaccgcc ccggtggtcg cgtccgaggc
     2521 catggccacc gcgaccacgg ccaggaccag caggtcgggc cggaccccgg ccaggcgcag
     2581 ctcggccagc acggtcgact ggacgagcac ggccacgacc aggacggcgg tcaggacgag
     2641 ggggcggcgc acggctcagc tcccccggac cggcggggcc gggcgctcga ccacgatcgc
     2701 gaccacgtcg agggcgccga gggcggcata cgggcggacc gcgacccggg ggaccaggct
     2761 ggccgcgccg gccgggtcga cccgctcgac caccccgacg ggtagcccgg ccgggaacac
     2821 gccgccctgg tagccctggg tgaccaccgg atcgccgcgg cggacggctg tgccggcccg
     2881 gaccggctgg aaggagagca gctgggcgcc gctgcccttg acgatgccgg gcgccttggc
     2941 gcgggccagg gtggccgcca ccccgctggc cgggtcggtg accagcagca cggtggcgta
     3001 gtcggcggtc acctgggtga cccgcccgac cagcccgtcg gcgtcgacca cggccatgtc
     3061 ctttgccacc ccctggcgcg acccggcgtc gaccgtgacc gacagctgga agttcgagcc
     3121 cgagctggcc accaccgagg ctccgacggt gcggcagccg cagcgggcgg ccatgcgcag
     3181 cgccccccgc aggcgccggt tctcccccag caggtcggcg taggtccgct cctgggcccg
     3241 cagccgggcc acctcggcct ccaggcggcg gttgtcctcg cgcagcccgc ccagctcgga
     3301 gaccccgccg gccaggtcgg caaccggccg cacgaccgcg ctggccccgc gctgcagcgg
     3361 cccgaacacg gcgatggcga ggcgctgcag gcggtcgacg gggccgccgt cgggctggcg
     3421 gaagtcgatc gtgatgagca cgatcgcggc ggccagcagc acggccagga cggtgcgcgc
     3481 gcggcgggga cttctcgaca ccttctccgg gaggcgcaga gcctcccgac ctcccccggg
     3541 tcagtgggcc tctgacgaga tgagcacttt cttgagcgac tcgaactc
//
LOCUS       DASSWR010000103         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_964801,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000103 DASSWR010000000
VERSION     DASSWR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964801"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1048)
                     /locus_tag="VFO47_01700"
     CDS             complement(185..1048)
                     /locus_tag="VFO47_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787447.1"
                     /translation="MRALPVALCGVVVGWGIGFWAAPQVLAGLARSSLARVNYRKREV
                     VAGLGLLLPLGLLVWAAPLAVAARVDPLRAGRAGILAPSALAVVVAGLAFVVLGLVDD
                     LVEDPAGSRGFRGHLRALASGRLTGGGIKLFGGALAGLLVASLAMPGDRPAWAILLGG
                     LVVASAANTANLLDLRPGRCAKVFLPLWVAGCLLDPGGGAWSAGLAGAALAALPFDLR
                     EEGMLGDAGANALGAVVGTLLLAGPMWLLWGAAAVLVALQLASERVSFTRVIEGNRVL
                     RAADRLGRRGT"
     gene            complement(1048..1815)
                     /locus_tag="VFO47_01705"
     CDS             complement(1048..1815)
                     /locus_tag="VFO47_01705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8787448.1"
                     /translation="MSAGTVLALVPAKDEADRVGATVRALRQLPGVAEVLVVSDGSTD
                     ATAARALEAGAHCLDLPRNVGKGGALNAGLAALMGRVAERLSPEPAVLLLADADLADT
                     AGRLGRLLDPVLAGEADLAIADLPAQQGAGGFGVAMGLARRGMARATGRRMAEPLSGQ
                     RAVRWEALPALLPFAPGFGVEVAMTMDALRAGLRVVEVEVDLHHNATGKDLAGLLHRA
                     RQARAIARELTRRRSWRPTRIPNGHPASPAEPPQDGG"
     gene            complement(1812..>2181)
                     /locus_tag="VFO47_01710"
     CDS             complement(1812..>2181)
                     /locus_tag="VFO47_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HET8787449.1"
                     /translation="GNDPFPRQGSLFVLLGPAAAATTAVAPDAFLVPLAGQVSTQTGG
                     PVAGGEAAAVPEQTSWIRALRANGEVSRLVSGIDSVDTVYGQLALVEALEGSLQQLPA
                     GQYGSKAGASGLLPERTESS"
BASE COUNT          283 a          842 c          854 g          202 t
ORIGIN      
        1 ccgcccgtca caaagatgtg cttcgccaag gtgcttgacc ccctttgggc ccatcggggg
       61 gaccgggtgt gctcccctgg ccacccggcc gtctgcgggc cctcccggga tgccagcgta
      121 gcacgaatgc aggagggctc cgcgcaggcc gcaccaaccc tgcgcgaagg tcctggtacg
      181 ggcgttaggt gccgcgccgg ccgagccggt cggcggcccg gagcacccgg ttgccctcga
      241 tcacgcgggt gaacgagacc cgctcggagg cgagctggag ggcgaccagg acggccgcgg
      301 ccccccacag cagccacatc gggccggcca ggagcagcgt gccgaccacg gcgccgaggg
      361 cgttggcccc ggcgtcgccg agcatgccct cctcgcgcag gtcgaagggg agggcggcca
      421 gggcggcccc ggccagcccg gccgaccagg ccccgccgcc ggggtcgagc aggcagccgg
      481 cgacccacag cggcaggaac accttggcgc agcggccggg tcgcaggtcg agcaggttgg
      541 cggtgttggc ggccgaggcc accaccagcc cgccgagcag gatcgcccac gccggccggt
      601 cgccgggcat ggccaggctg gccaccagca gcccggcgag ggcgccgccg aacagcttga
      661 tccccccgcc ggtgagccgc cccgaggcca gcgcccgcag atggccccgg aagccgcggc
      721 tgcccgccgg gtcctcgacc aggtcgtcga ccagccccag gaccacgaac gcgagcccgg
      781 ccacgaccac ggccagcgcc gacggggcca ggatcccggc ccgccccgcc cgcagcggat
      841 cgacccgcgc ggccacggcc agcggcgccg cccacaccag cagcccgagc ggcagcagca
      901 gcccgagccc ggccaccacc tcccgcttgc ggtagttcac ccgcgccagg ctggaccggg
      961 ccagcccggc cagcacctgc ggcgccgccc agaacccgat cccccacccc accaccaccc
     1021 cgcacaacgc caccggcagc gcccgcatca gccaccgtcc tgggggggct cggcagggga
     1081 ggcggggtgg ccgttgggga tgcgggtggg gcgccaggat cggcggcggg tgagctcgcg
     1141 ggcgatggcg cgggcttggc gggcgcggtg gaggaggccg gcgaggtcct tgccggtggc
     1201 gttgtggtgg aggtcgacct cgacctcgac cacgcggagg ccggcgcgga gggcgtccat
     1261 ggtcatggcg acctcgaccc cgaacccggg ggcgaacggc agcagggccg ggagggcctc
     1321 ccagcggacc gcgcgctggc cggacagggg ctcggccatg cggcggccgg tcgcccgggc
     1381 catgccgcgg cgggccaggc ccatggcgac gccgaacccg ccggcgccct gctgggcggg
     1441 gaggtcggcg atggccaggt cggcctcgcc ggccaggacc gggtcgagca ggcggccgag
     1501 ccgcccggcc gtgtcggcca ggtcggcgtc ggccagcagc agcaccgccg gctcggggct
     1561 gagccgctcg gcgacccggc ccatcagggc ggccagcccg gcgttgaggg cgccgccctt
     1621 gccgacgttg cggggcaggt cgaggcagtg ggccccggcc tccagggccc tggccgcggt
     1681 ggcgtcggtc gacccgtcgc tgaccaccag cacctcggcc accccgggca gctggcgcag
     1741 ggcccgcacg gtcgccccga cccggtcggc ctcgtccttg gccggcacca gggccagcac
     1801 cgtcccggcg ctcacgagct ctcggtccgt tccgggagga ggccggaggc accggccttg
     1861 ctgccgtact ggcccgcggg gagctgctgg aggctcccct cgagggcctc gaccagggcc
     1921 agctggccgt agacggtgtc gaccgagtca atgccggaga ccaggcggct gacctccccg
     1981 ttggcccgaa gggcgcggat ccaggaggtc tgctcgggga cggcggcggc ctcgcccccg
     2041 gccaccgggc cgccggtctg ggtcgagacc tggccggcca gggggacgag gaaggcgtcc
     2101 ggggccacgg ccgtggtggc ggcggcggcc gggccgagca gcacgaacag cgagccctga
     2161 cgggggaacg ggtcgttccc g
//
LOCUS       DASSWR010000104         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_230431,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000104 DASSWR010000000
VERSION     DASSWR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230431"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFO47_01715"
     CDS             complement(<1..1002)
                     /locus_tag="VFO47_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8787450.1"
                     /translation="MALLVWAVAAPAFTPGGALAAGPVAATAGGAIATGDPDPAEWAR
                     LRVLAWQRGREAAATAGRDRAAARRAAEGAGATATPASWVVPAGGGATIPALTLRAYR
                     EAAAWAAGFDPDCRLSWTVLAGIGRIESNHGLFGGPATRFSAAGTVSPRITGPPLDGR
                     GVARIPDSDGGRWDGDATWDRAVGPMQFIPTTWRSLGRDGNGDRVADPNNLFDAAVSA
                     AGYLCLSGDGDLVDPARLRQAIYAYNHSSSYVDAVLGWARLYQGGVTAGPAVPSGPPA
                     PTTTAGPPATGVGGQPTTTTRATTTRPPTTTRPTSTTHRPTTTTDPPTTTRPSTTTPT
                     "
     gene            1406..>2787
                     /locus_tag="VFO47_01720"
     CDS             1406..>2787
                     /locus_tag="VFO47_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET8787451.1"
                     /translation="MTVKAKDRKELRSFGLVTVVAALAAALFLVWVGLGIGGPATTTL
                     VSNLFQAGTALIAASACFSAATRTPYGFGRAWTASLHRVWRLLGSAALAWGLGQVIWT
                     YLEWRTGEEPGVPSLADVGYLTAVPLLIAGVLAFPIAPMRATARLRTLLDGLLIAASL
                     LFLGWATVLGDAFRDNGTSSTLERAICLAYPLGDIVAGTIVLVLLTRSRGRGVIHLSM
                     IAAAVFSLLIADLGFAYRIPTDTYTTGAMVDAGWVVGWLLLTVTALKPTVAELRRTDD
                     EEEPSITRLALPYAPLVVAAVTALIIQIVTGEFEPFLVYTGTMIGLLVISRQIVALIE
                     NRQLNANLRTTVMQLSEREQELKDSLRRELAAADRLRAADAMKDTFLRAVSHDLRTPL
                     TAMLGVAVTLERTRLNLPREQALDLVGMLVEKTRKLERLLKDLLDLNRLEEGVLEPNR
                     SLTDVRELVHR"
BASE COUNT          340 a         1026 c         1016 g          405 t
ORIGIN      
        1 ggtcggggtg gtcgtcgacg gacgggtcgt ggtcggcggg tcggtggtgg tggtcggccg
       61 gtgggtggtg gaggtcggcc gggttgtggt cggcggccgg gtcgtggtgg cccgggtggt
      121 cgtggtgggc tggccaccca ccccggtggc cggcggtccg gcggtcgtgg tcggggccgg
      181 cgggccggag ggcacggccg ggccggccgt gaccccgccc tggtagaggc gggcccagcc
      241 gagcaccgcg tccacgtacg aggaggagtg gttgtaggcg tagatcgcct ggcgcagccg
      301 ggccgggtcg accaggtcgc cgtcgccgct caggcacagg tacccggcgg cgctcaccgc
      361 ggcgtcgaac aggttgttgg ggtcggcgac ccggtcgccg ttgccgtcgc ggcccagcga
      421 ccgccaggtc gtcgggatga actgcatcgg cccgaccgcc cggtcccagg tcgcgtcccc
      481 gtcccagcgg ccgccgtcgc tgtcggggat gcgggccacc ccgcggccgt ccagcggcgg
      541 gccggtgatc ctcgggctca ccgtcccggc cgcgctgaac ctggtcgccg gccccccgaa
      601 caggccgtgg ttggactcga tgcggccgat gccggccagg accgtccagg agagccggca
      661 gtccgggtcg aacccggccg cccaggcggc ggcctcgcgg taggcgcgca gggtgagggc
      721 ggggatggtg gcgccgcctc cggccgggac cacccaggag gcgggcgtgg cggtggcgcc
      781 ggctccctcg gccgcccgcc gggccgccgc ccggtcgcgt ccggccgtgg ccgccgcctc
      841 gcgtccccgc tgccaggcca ggacgcgcag acgggcccac tcggcggggt cagggtcacc
      901 ggtcgcgatg gcgcctccgg ccgtcgcggc caccggcccg gcggccaggg ccccgcccgg
      961 ggtgaacgcg ggcgccgcga cggcccagac cagcagcgcc accgcggcgg cgcctccggc
     1021 ccgcaggcgg cggacgaagg gcgcggacgg cggtggcggt cggtgcaaag ggcggctccc
     1081 gtcccgggga cgtgcgcggc cttccatgtt ggcacgtcat ggccgagaat gccgccccaa
     1141 gatgttagtg acagctatgt tcaggcaaga accaagcgtc taggtcgccc ttgtcgcgac
     1201 gatgtagagt ttggcggttg ggaacgtggg ggaaaggtgg cgatggtcga gcaggaaaac
     1261 ctcgccggat acctagttct tcgtgaccag gtgacgggaa cgtcgccctc tgctcgtgta
     1321 tcgtatggac tcgccttgca ccctattgac ggcgttcagg aagtgcacag ctcgagctca
     1381 ttcgtggggc ccccgtggtg aaaccgtgac ggtcaaggcc aaggaccgga aggagctgcg
     1441 cagcttcggc ctggtcaccg tcgtcgcggc gctggcggcg gccttgttcc tggtctgggt
     1501 cgggctcggg atcggcggtc ctgcgaccac caccttggtc agcaacctct tccaggcggg
     1561 caccgccctg atcgccgcca gcgcctgctt cagcgccgcc acccgcaccc cctacggctt
     1621 cggccgggcc tggaccgcct ccctgcaccg cgtctggcgc ctgctcggct cggccgccct
     1681 ggcctggggc ctcggccagg tcatctggac ctacctggaa tggcgcaccg gcgaggagcc
     1741 gggcgtgccg tcgctggccg acgtcggcta cctgaccgcg gtgccgctgc tgatcgccgg
     1801 ggtgctggcc ttcccgatcg ccccgatgcg ggccacggcc cgcctgcgga ccctgctcga
     1861 cggcctgctg atcgccgcct ccctgctgtt cctcggctgg gccaccgtgc tcggcgacgc
     1921 cttccgcgac aacggcacca gctcgacctt ggagcgggcc atctgcctgg cctacccgct
     1981 gggcgacatc gtggccggca ccatcgtgct ggtgctgctc acccgctccc gcggccgcgg
     2041 cgtcatccac ctgtcgatga tcgccgccgc ggtgttctcc ctgctcatcg ccgacctcgg
     2101 cttcgcctac aggatcccaa ccgacaccta caccaccggg gccatggtcg acgccggctg
     2161 ggtggtgggc tggctgctgc tgaccgtcac cgccctcaag cccaccgtgg ccgagctgcg
     2221 ccgcaccgac gacgaggagg agccgtcgat cacccggctc gccctgccgt acgcgccgct
     2281 ggtcgtggcc gccgtcaccg ccctgatcat ccagatcgtc accggcgagt tcgagccgtt
     2341 cctggtctac accggcacca tgatcgggct gctggtcatc tcccgccaga tcgtcgccct
     2401 gatcgagaac cgccagctca acgcgaatct caggaccacc gtcatgcagc tgtccgagcg
     2461 cgagcaggag ctcaaggaca gcctgcggcg cgagctggcc gccgccgacc ggctgcgggc
     2521 ggccgacgcc atgaaggaca ccttcctgcg cgccgtctcc catgacctgc gcaccccgct
     2581 cacggccatg ctcggggtcg ccgtcaccct ggagcggacc cggctcaacc tgccccggga
     2641 gcaggccctt gacctggtcg ggatgctggt cgagaagacc cggaagctgg agcggctgct
     2701 caaggacctg ctcgacctca accggctcga ggagggcgtg ctcgagccga accgctccct
     2761 caccgacgtc cgcgagctcg tccaccg
//
LOCUS       DASSWR010000105         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_512690,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000105 DASSWR010000000
VERSION     DASSWR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512690"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFO47_01725"
     CDS             <1..364
                     /locus_tag="VFO47_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787452.1"
                     /translation="RLDLLRFEGGLLLRERSSMVYIYGVTVQVVAGALLALVYRQVFQ
                     QLDSATFVGWGALLGLAQGVLAVLALPLLGRLNRNVRAGDEPRPGQFGRAYGGLTPVA
                     LVVASVVFGTWVGIVLIP"
     gene            361..1095
                     /locus_tag="VFO47_01730"
     CDS             361..1095
                     /locus_tag="VFO47_01730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET8787453.1"
                     /translation="MSTLVLLREQIAANRRAYQLVVAVVAGGLALLGLLVTLVLGLGV
                     SGLGAGLGLAVLVVGLAPGLGERAALRDAGAQPADPVRWPRYHNLVEGLSQAAGLPVP
                     RLYVVDSDAANAFSVGRDPSRSALVVTRGLLTTLNRVELEGVLAHELNHIWSWGARLA
                     TLGVALGRVPGLLRLLAPPRREFDADEGGARLTRYPPGLVSALRKLASGGQPPDGPGL
                     RHLWIMAPGDATHPPVDERIAALHEL"
     gene            complement(1028..2251)
                     /locus_tag="VFO47_01735"
     CDS             complement(1028..2251)
                     /locus_tag="VFO47_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8787454.1"
                     /translation="MAHGAAVLLELGGVILGLGILGRLAGRLGLSAIPRYLLAGLAFG
                     EGGLLPLVTAQEFIEIGAEVGVILLLLLLGLEYSASELMSSLRAAAPAGLVDLALNFT
                     PGLLAGLLLGWSALAAVVLGGVTYVTSSGIVAKVLGDLNRLGNRETPVILSILVVEDL
                     AMAAYLPLVTGLLVGGGLLASVLPVGVALATVAVVLVVAVRYGETLSRLVFSPSDEVL
                     LLVILGVALVVAGLAQQLQVSAAVGAFLVGIALSGRAAEAAHDLLSPLRDLFAAVFFV
                     FFGLQTDPRSIPPVALVATVLALVTAATKVATGWWAAKRIGVGPRGRWRAGTALVPRG
                     EFSIVIAGLAVAAGREAELGPLATAYVLILAVLGPVLARTSEPLRVGLRRTLTARAGR
                     RSARPPADAWRPREP"
BASE COUNT          298 a          881 c          863 g          271 t
ORIGIN      
        1 ccggctggac ctgctccgct tcgagggcgg cctgctgctc cgggagcgga gcagcatggt
       61 ctacatctac ggggtgaccg tgcaggtcgt ggccggcgcc ctgctcgccc tggtctaccg
      121 gcaggtgttc cagcagctcg actcggccac cttcgtgggc tggggagccc tgctcggcct
      181 cgcccagggg gtcctggccg tgctggccct gccgctgctg ggccggctca accgcaacgt
      241 gcgggccggc gacgagccgc ggcccggcca gttcgggcgc gcctacggcg gcctcacgcc
      301 cgttgccctg gtggtcgcct cggtggtctt cggcacctgg gtcggcatcg tcctgattcc
      361 ttgagcaccc tcgtcctcct gcgggagcag atcgccgcca accggcgcgc ctaccagctc
      421 gtcgtggccg tggtggccgg tggcctggcc ctgctcgggc tgctggtcac cctggttctc
      481 ggcctcgggg tgagcgggct cggggccggg ctcggcctgg ccgtgctggt cgtcgggctc
      541 gcccccgggc tgggggagcg ggccgccctg cgcgacgccg gcgcccagcc ggccgacccg
      601 gtccgctggc cccgctacca caacctggtc gagggcctga gccaggccgc cggcctgccc
      661 gtgccccggc tgtacgtggt cgacagcgac gccgccaacg ccttctcggt cgggcgcgac
      721 ccctcccggt cggccctggt cgtcacccgg gggctgctca ccaccctgaa ccgggtcgag
      781 ctggagggcg tgctcgccca cgagctcaac cacatctgga gctggggggc gcgcctggcc
      841 accctcgggg tcgccctcgg ccgggtcccg gggctgctgc ggctgctggc cccgccccgg
      901 cgcgagttcg acgccgacga gggcggggcc cgcctcaccc gctacccgcc gggcctggtc
      961 agcgccctca ggaagctggc ctcgggcggc cagccgccgg acgggcccgg cctgcgccac
     1021 ctctggatca tggctcccgg ggacgccacg catccgccgg tggacgagcg gatcgccgcc
     1081 ctgcacgagc tgtgagcgtt cgcctgaggc cgactcgcag gggctcggag gtccgggcca
     1141 ggaccgggcc caggacggcc aggatgagca cgtaggcggt ggccagcggg cccagctcgg
     1201 cctcgcggcc ggcggccacg gccaggccgg cgatcacgat ggagaactcg cccctgggga
     1261 ccagggccgt gccggcccgc cagcgccccc gggggccgac cccgatccgc ttggccgccc
     1321 accagccggt ggcgaccttg gtggccgccg tcaccagggc caggacggtg gccacgagcg
     1381 cgaccggggg gatcgagcgc gggtcggtct ggagcccgaa gaacacgaag aacacggccg
     1441 cgaacaggtc gcgcaggggg ctgagcaggt cgtgggcggc ctcggcggcc cggccggaca
     1501 gggcgatgcc gaccaggaac gccccgaccg cggccgacac ctggagctgc tgggccagcc
     1561 cggccacgac cagggccacg ccgaggatga ccagcagcag cacctcgtcg ctggggctga
     1621 acaccagccg gctgagggtc tcgccgtagc ggaccgcgac caccaggacc acggccaccg
     1681 tggccagggc gaccccgacc ggcaggacgc tggccagcag cccgcctccg accagcagcc
     1741 cggtgaccag cggcaggtag gcggccatgg ccaggtcctc gacgaccagg atggacagga
     1801 tcaccggggt ctcgcggttg ccgagccggt tcaggtcgcc gaggaccttg gccacgatcc
     1861 ccgacgaggt cacgtacgtc accccgccca gcaccaccgc ggccagggcc gaccagccga
     1921 gcagcagccc ggccagcagg ccgggggtga agttgagggc gaggtcgacc aggccggccg
     1981 gggcggcggc gcggaggctg ctcatcagct cgctggccga gtactccagc cccagcagga
     2041 gcagcaggag gatgaccccg acctctgccc cgatctcgat gaactcctgg gcggtgacca
     2101 gcggcagcag gccgccttca ccgaaggcca gcccggccag caggtaccgg gggatggcgg
     2161 agaggccgag gcggccggcc agccgtccca ggatgcccag ccccaggatc accccgccca
     2221 gctcgagcag gacggcggcg ccgtgggcca cgccggtcag ctccccagca gcttggcgag
     2281 ctccttgatg ccctgggcgg tgccgacgac cac
//
LOCUS       DASSWR010000106         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1021486, whole genome shotgun sequence.
ACCESSION   DASSWR010000106 DASSWR010000000
VERSION     DASSWR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021486"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1497)
                     /locus_tag="VFO47_01740"
     CDS             complement(73..1497)
                     /locus_tag="VFO47_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET8787455.1"
                     /translation="MHPHRNYPRQPVGWGALHEAPPPVIAPYVEIRPADWLLLAVLRP
                     AWAYRLELALIVLVAVAYVWLGGKLGRADADRLIVAAAFVVALLGWTREPLARSLARS
                     HVRRRWALACRHAELASRNDRVPWVMRCALTRAGEQLRARLPAGAQVPDLENASERLA
                     AFLAVREVRVTRDPANARYARVVVLRRDPLADPTPIPWPLANADRCSLWESIRVGEDE
                     DGNEVTVTLPERNLLAGGEPGAGKSTVVQLLIAAAALDPTVVLTLFDPKLVELAVWQG
                     CAHRLVGPNVEEAIDVLKGLIGELDDRYLTLLANRARKVAEGDGLPLYLVVIEELAYF
                     TNGPDRKTAQAFSTLLRDFVARGRAAGMVTVATTQKPSADVVPTYLRDLFGFRWALRC
                     STPEASDTILGRGWASQGYSAAQIDPAARGVGLLLQEGGIPVRLRSCYLDDLDLADLA
                     RRAEALRDNDRSDTPTLRLLDGTG"
     gene            complement(1635..1958)
                     /locus_tag="VFO47_01745"
     CDS             complement(1635..1958)
                     /locus_tag="VFO47_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787456.1"
                     /translation="MKLPIDTSAIAFLCAMPPEPVVDFDTKRPRADENGEPLYVVQLL
                     VMGEDSADLIAVKVSGVPSSAIRQGVPVKVSGLVAQPWTMADRSGVSFRASRIEPAMA
                     AAKAS"
BASE COUNT          381 a          788 c          833 g          321 t
ORIGIN      
        1 agccggggcc ggcccggtcg atgacctcct ggagaacacc cggcagcagc tccccaacca
       61 gagcaggctc gctcatccgg tgccgtccag cagccgcagc gtcggcgtat cggacctgtc
      121 gttatcgcgg agggcctcag cgcggcgagc caggtcggcc aagtccaagt catcgaggta
      181 gcaggaccgc aaccgcaccg ggatgccacc ctcctgcagc agcagtccca ccccgcgggc
      241 tgccgggtcg atctgggcgg ccgagtagcc ttggctggcc cagccccgac ccaggatggt
      301 gtcgctggcc tcgggggtgg agcacctgag cgcccaccgg aacccgaaca ggtcacggag
      361 ataggtcggg accacatcgg ccgagggctt ctgggtggtg gccaccgtga ccatgccggc
      421 ggctcggccc cgggccacga agtcgcgcag cagggtcgag aaggcttggg cggtcttacg
      481 gtcggggccg ttggtgaagt aggccagctc ctcgatcacc actaggtaca gcggcagtcc
      541 atcaccctcg gcgaccttgc gggctcggtt ggccagcagg gtcaggtaac ggtcgtccag
      601 ctcgccgatc aggcccttga ggacgtcgat ggcctcctcg acgttggggc cgaccagccg
      661 gtgagcgcag ccttgccaga cggccagctc gaccagcttg ggatcgaaca gggtcaggac
      721 caccgtgggg tcgagggcgg cggccgcgat caggagctgc accacggtgc tcttgccggc
      781 ccccggctcc ccgccggcca gcaggttgcg ctccggcagg gtgacggtga cctcattccc
      841 gtcctcgtcc tcgccgaccc ggatcgactc ccacagcgag cagcggtcgg cgttggccag
      901 cggccaggga atcggggtcg ggtcggccag cgggtcacgg cgcaggacca ccacccgcgc
      961 atagcgtgcg ttggctgggt cacgggtgac gcggacctct cggacggcca ggaaggcggc
     1021 caaccgctcc gaagcattct ccaggtcggg cacctgggcg ccggccggga gccgggcccg
     1081 gagctgctcg ccggcgcggg tgagcgcaca gcgcatgacc catggcaccc gatcgttccg
     1141 agaggccagc tcagcgtggc ggcaggccag cgcccaccgt ctgcggacat gggagcgggc
     1201 caacgagcgg gccagcggct cccgcgtcca ccccagcagg gccaccacga acgcggcggc
     1261 cacgatgagg cggtcagcat cggcgcggcc gagcttgcca cccagccaca cataggccac
     1321 cgccaccagg acgatcaagg cgagttcgag gcgataggcc caggccggcc ggaggacggc
     1381 cagcagcagc cagtcggccg ggcggatctc gacgtaaggc gcgatgaccg ggggtggggc
     1441 ttcgtgtagg gcgccccagc ccaccggctg gcgggggtag ttacgatgag ggtgcatcag
     1501 gggctccact tgatgcaggc caggtgttgc gagcacctgg tcaggtggtg gaggggccgg
     1561 cccggctccc aacagccggc ctgccggccc ctccgtcgtt ccgccgctct tgtctctggc
     1621 atccctccct tcggttagct cgccttcgcc gccgccatgg ccggctcaat gcgggacgca
     1681 cggaatgaca ccccggagcg gtcggccatc gtccagggct gggcaaccag ccccgagacc
     1741 ttgaccggga caccctggcg gatggccgag gacggcaccc cggagacctt gaccgcgatc
     1801 aggtcggccg agtcctcgcc catgaccagg agctggacga catagagcgg ctcgccgttc
     1861 tcgtcggcgc gaggccgctt ggtgtcgaag tcgaccaccg gctcgggcgg catcgcacac
     1921 aggaacgcga tcgccgacgt gtcgatcggg agcttcattg actgcctcct tccgcaacga
     1981 cgctgtgtgc ggccaggaaa cagccatcaa tgaaagttca gaaggtccgc aagcaggtcc
     2041 ccgagccggt ccgttgacag gtcccgccga ggatcgtgac caaagtgttg gtgggtgcgg
     2101 ctacgctgac ggggtccggg cttggaggaa cggcagttat gagtgctgcg aggatctacg
     2161 tccccaatgt gcagcttcga gagcaccggc tacgcctcaa cctgacgctg gaggaggtcg
     2221 ccgaggcgct caccgcactg gcctgggaac ggttcgccgt ccgtgtgggc gtaaacgcag
     2281 ggatggttgg caaatgggag cggggcgaga agcggcccag ccg
//
LOCUS       DASSWR010000107         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1219423, whole genome shotgun sequence.
ACCESSION   DASSWR010000107 DASSWR010000000
VERSION     DASSWR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219423"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFO47_01750"
     CDS             complement(<1..626)
                     /locus_tag="VFO47_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HET8787457.1"
                     /translation="MTRRQLVLLAVWLLLAVLLTPAAGLGVASRASAGSQAQATVTVM
                     AAGDISPDPSASKDDDVATSDLILDADPTAVLTLGDNQYPRGELADFRSRSGYAGSWG
                     RFKARTHPSPGNHDYSDPAGGAAGYFGYVGRLAGDPAKGYYSFDLGAWHIIALNSNCG
                     AAGAPSCDQDSAQVRWLQADLEGNAKRCTLAYWHHPRYTDPAGHRDDPR"
     gene            complement(848..2329)
                     /locus_tag="VFO47_01755"
     CDS             complement(848..2329)
                     /locus_tag="VFO47_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8787458.1"
                     /translation="MAEQLQRRTEQTPARFGLDYAPAPEATDHVQLRSDHGLLIGGRW
                     VEPVAQKRFKTINPATEEVLAEVAEAGEEDVDAAVRAARAAYTKVWSKLSGAERAKYL
                     YRIARALQERAREFAVLETINGGKPIKESRDVDVPLAAAHFFYHAGWADKLDYAFPGR
                     RPAPLGVAAQVIPWNFPLLMAAWKLAPALAAGNTVVLKPAETTPLTALRLGEVLLEAE
                     LPEGVVNILTGAGATGAAVVGHAGVDKVAFTGSTEVGKAIQRTLAGSGKRLTLELGGK
                     AANIVFEDAPLDQAVEGIVNGIYFNQGHVCCAGSRLLVQESIAEPLVDKLKRRMATLR
                     VGDPLDKNTDVGAINSRAQLDKITELVASGEADGAQAWQAPCELPQRGFWFRPTVFTH
                     VAQSYRIAQEEIFGPVLSVLTFRTPDEAVEKANNTPYGLSAGVWTDKGSRILDLASRM
                     RAGVVWANTFNRFDPTSPFGGYRESGFGREGGRHGLAAYLDLS"
     gene            complement(2336..>2431)
                     /locus_tag="VFO47_01760"
     CDS             complement(2336..>2431)
                     /locus_tag="VFO47_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET8787459.1"
                     /translation="LGASSLLNDCLMQLAKERSGRYQSGDYFTKD"
BASE COUNT          332 a          825 c          918 g          356 t
ORIGIN      
        1 cgcgggtcgt cccggtgccc ggccgggtcg gtgtaccgcg ggtgatgcca ataggccagc
       61 gtgcaccgct tggcgttgcc ctccaggtcg gcctgcagcc acctgacctg ggctgagtcc
      121 tgatcgcagg acggcgcgcc cgcagccccg cagttggagt tgagcgcgat gatgtgccag
      181 gcgcccaggt cgaacgagta gtagcccttg gcgggatcac cggccagccg gccgacgtag
      241 ccgaagtagc cggccgcccc gccggccggg tcgctgtagt catggttccc tggcgagggg
      301 tgggtcctgg ccttgaaccg tccccacgac ccggcgtagc cggaccggct gcggaagtcg
      361 gccagctccc ccctgggata ctggttgtcg ccaagggtga gcacggcggt cgggtcggcg
      421 tccaggatga ggtcgctggt ggcgacgtcg tcgtccttgc tggcgctggg gtcgggagag
      481 atgtcgccgg cggccatgac cgtgaccgtc gcttgggcct ggctccccgc cgacgcccgc
      541 gatgcgaccc cgagcccggc ggcgggcgtg agcagcacgg caagcagcag ccagacggcc
      601 agcaggacga gttggcgtcg agtcatacag cccggacctc tcaagacggc cagaggaccg
      661 tcacagcttc ggggtcaggg agaaactagc cgcaggcagc accaggacag cctccggaac
      721 ggtgtcatcc aagcccctac aggagatcgc tgggatcccg cgatccaggg ctcccgcgaa
      781 ggccgagtgg cctacgaccc agtgtgcggc gcaccgcacc tggatcacgg gggagggccg
      841 agggcggcta ggacaggtcg aggtaggcgg ccaggccgtg gcggccgccc tcgcggccga
      901 agccggactc gcggtagccg ccgaagggcg aggtggggtc gaagcggttg aaggtgttgg
      961 cccacacgac cccggcccgc atgcggctgg ccaggtcgag gatgcggctg cccttgtcgg
     1021 tccagacccc ggccgacagt ccgtaggggg tgttgttggc cttctccacc gcctcgtcgg
     1081 gggtgcggaa ggtcagcacc gacaggaccg gcccgaagat ctcctcctgg gcgatccggt
     1141 aggactgggc gacgtgggtg aacacggtgg ggcggaacca gaagccgcgc tggggcagct
     1201 cgcagggggc ctgccaggcc tgggcgccgt cggcctcgcc ggaggcgacc agctcggtga
     1261 tcttgtccag ctgggcgcgg gagttgatgg ccccgacgtc ggtgttcttg tccagcgggt
     1321 cgccgacccg cagggtcgcc atgcgccgct tcagcttgtc gaccagcggc tcggcgatcg
     1381 actcctggac cagcaggcgc gacccggcgc agcacacgtg gccctggttg aagtagatgc
     1441 cgttgacgat cccctcgacc gcctggtcga gcggggcgtc ctcgaacacg atgttggccg
     1501 ccttgccgcc cagctccagg gtgagcctct tgcccgaccc ggccagggtg cgctggatcg
     1561 ccttgccgac ctcggtcgag ccggtgaagg cgaccttgtc gacgccggcg tggccgacca
     1621 cggccgcccc ggtcgccccg gcgccggtga ggatgttgac cacgccctcg ggcagctcgg
     1681 cctccaggag gacctcgccc aggcgcaggg cggtcagcgg ggtcgtctcg gccggcttga
     1741 ggaccacggt gttgccggcg gccagggccg gggccagctt ccaggcggcc atcagcagcg
     1801 ggaagttcca ggggatgacc tgggcggcca cccccagggg ggccgggcgg cggccgggga
     1861 aggcgtagtc gagcttgtcg gcccagccgg cgtggtagaa gaagtgggcc gcggccaggg
     1921 gcacgtcgac gtcgcgggac tccttgatcg gcttgccgcc gttgatcgtc tccaggacgg
     1981 cgaactcccg ggcccgctcc tgcagggccc gcgcgatccg gtagaggtac ttggcccgct
     2041 cggcccccga cagcttcgac cagaccttgg tgtaggcggc ccgggcggcc cggacggccg
     2101 cgtccacgtc ctcctcgccg gcctcggcga cctcggccag gacctcctcg gtggccgggt
     2161 tgatggtctt gaaccgcttc tgtgcgaccg gctcgaccca ccgccccccg atcagcagcc
     2221 cgtggtcgga ccgcagctgc acgtggtcgg tcgcctcggg cgcgggcgcg tagtcgagcc
     2281 cgaaccgcgc cggggtctgc tcggtccgcc gttggagctg ctctgccatg tcgacttagt
     2341 ccttggtgaa gtagtcgccg gactggtagc ggccggagcg ctccttggcc agctgcatga
     2401 ggcagtcgtt gaggaggctg gaggcgccga g
//
LOCUS       DASSWR010000108         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_541343,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000108 DASSWR010000000
VERSION     DASSWR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541343"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..319
                     /locus_tag="VFO47_01765"
     CDS             116..319
                     /locus_tag="VFO47_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET8787460.1"
                     /translation="MLVTARLRPPDRRVEVADVRRVSDLLARLEVLPGAVLVIRDGAL
                     LTSQDELHDGDQVEVRFAISGGS"
     gene            322..1266
                     /locus_tag="VFO47_01770"
     CDS             322..1266
                     /locus_tag="VFO47_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787461.1"
                     /translation="MAMRCKRCRGTAVMEVRRHNAAFCRDCFFHYVGQQVTRAIAKYR
                     MLGHTDRVLVAVSGGKDSLALWDLLLELGYQVDGLYLSLGIGAYSERSKDVCQEFAAA
                     RGARLLVHDLAGEHGYTIPEAAGKGGRSSCGVCGLSKRYVFNRAALDGGYDVVCTGHN
                     LDDEAAVLFGNTLRWEVAAMARQFPVLEATRPGLVKKVKPLYRVAERETAAYCVLKGI
                     DYVVEECPLVAGNTQMRHKETLNQMEEAAPGTKHSFLFGYLDRAAGLLKVADVADLTE
                     CARCGMVTQAPDDPASEAVCAFCRSKSRLVTSLPVVTA"
     gene            1263..2072
                     /locus_tag="VFO47_01775"
     CDS             1263..2072
                     /locus_tag="VFO47_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HET8787462.1"
                     /translation="MRPLEAGERVLLQDVKGRRYLITLEQGATFHTHKGRLAHDELIG
                     SAEGAVARTDLGQKLLVLRPTLADWVVKMPRGAQVIYPKMLALMVMAADVQPGMTVVE
                     AGTGSGALSIALLQALAPGGRLHSFERRPDFADQARRNLERWFGKPPDGFELHQGDVV
                     EGIAGVGPVDRVLLDLLEPWLVVPGAAGALHPGGVLVCFVATVPQVMRLTEACESSGS
                     FGLVETSEAIFRPWHVDGLAVRPEHRMVGHTGFLVSARRVDGPWPSPGRVP"
BASE COUNT          288 a          830 c          793 g          312 t
ORIGIN      
        1 gctgccccag gtcgtcctcg gcctgttcct gtggcgggcc gccgccgacg gcgaacgctc
       61 ggccgcccgc tcggaactgc tcgccgggcg ggccgagcgc gggtagcctt ctggcatgct
      121 cgtcaccgcc cgcctgcggc cgcccgaccg tcgcgtcgag gtggccgacg tccgccgcgt
      181 cagcgacctg ctggcccgtc tcgaggtgct ccccggggcg gtcctggtga tccgtgacgg
      241 cgccctgctg accagccagg acgagctcca cgacggcgac caggtcgagg tccggttcgc
      301 gatcagcgga ggttcatagc aatggcgatg cgctgcaagc gctgccgcgg caccgccgtg
      361 atggaggtgc gccgccacaa cgccgccttc tgccgcgact gcttcttcca ctacgtcggc
      421 cagcaggtga ccagggccat cgccaagtac cggatgctcg gccacaccga ccgggtgctg
      481 gtcgcggtct ccggcggcaa ggactccctg gccctctggg acctgctgct ggagctcggc
      541 taccaggtcg acggcctcta cctcagcctc ggcatcggcg cctactccga gcgctccaag
      601 gacgtgtgcc aggagttcgc ggccgcccgc ggggcccggc tgctggtcca cgacctggcc
      661 ggcgagcacg gctacacgat ccccgaggcg gccggcaagg gcggccgctc ctcctgcggg
      721 gtgtgcgggc tgtccaagcg ctacgtcttc aaccgggccg ccctcgacgg cggctacgac
      781 gtggtctgca ccggccacaa cctcgacgac gaggcggcgg tgctgttcgg caacaccctg
      841 cgctgggagg tggcggcgat ggcgcgccag ttcccggtcc tggaggcgac ccggcccggc
      901 ctggtcaaga aggtcaagcc gctgtaccgg gtggccgagc gcgagacggc cgcctactgc
      961 gtgctcaagg gcatcgacta cgtggtcgag gagtgcccgc tggtcgccgg caacacccag
     1021 atgcgccaca aggagaccct caaccagatg gaggaggcgg cccccgggac caagcacagc
     1081 ttcctgttcg gctacctgga ccgggccgcc ggtctgctca aggtggccga cgtggccgac
     1141 ctcaccgagt gcgcccgctg cggcatggtc acccaggccc ccgacgaccc ggcctccgag
     1201 gccgtctgcg ccttctgccg ttccaagtcc cggctggtca cgtccctgcc ggtggtgact
     1261 gcttgaggcc gctcgaggcg ggggagcggg tgctgctcca ggacgtcaag gggcgccgct
     1321 acctgatcac cctggagcag ggggcgacct tccacaccca caagggccgc ctggcccacg
     1381 acgagctgat cgggtcggcc gagggcgccg tggcccgcac cgacctcggc cagaagctgc
     1441 tggtgctgcg gcccaccctg gccgactggg tggtcaagat gccccgcggg gcccaggtca
     1501 tctaccccaa gatgctggcc ctgatggtga tggcggccga cgtccagccg ggcatgaccg
     1561 tggtcgaggc cggcaccggc tccggggcgc tgtcgatcgc cctgctccag gcgctcgccc
     1621 ccggcgggcg gctgcactcc ttcgagcgcc gccccgactt cgccgaccag gcccggcgca
     1681 acctcgagcg ctggttcggc aagccccccg acggcttcga gctgcaccag ggcgacgtcg
     1741 tcgagggcat cgccggggtc gggcccgtgg accgcgtcct gctcgacctg ctcgagccgt
     1801 ggctggtcgt gcccggggcg gccggcgccc tgcacccggg cggggtgctg gtctgcttcg
     1861 tggccaccgt gccccaggtg atgcgcctga ccgaggcgtg cgagtcctcg ggcagcttcg
     1921 gcctggtcga gacctcggag gccatcttcc gcccctggca cgtcgacggc ctggccgtcc
     1981 gccccgagca ccgcatggtc ggccacaccg gcttcctggt ctcggcccgc cgggtcgacg
     2041 gcccctggcc gtcccccggc cgcgtcccct gacccgtttg cgcgggagga tcagggggca
     2101 ggcagcccgc ggaggtgatg cccacgtggc gaccgacgac aagcttcgca accagggcga
     2161 ggagctgaag ggcaaggcca aggagtccct cggccgcgcc accgacgacg aggagctgga
     2221 ggc
//
LOCUS       DASSWR010000109         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1049774, whole genome shotgun sequence.
ACCESSION   DASSWR010000109 DASSWR010000000
VERSION     DASSWR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049774"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1026)
                     /locus_tag="VFO47_01780"
     CDS             complement(19..1026)
                     /locus_tag="VFO47_01780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET8787463.1"
                     /translation="MPPQQRPAARERAVDPARSRVSGTSVGMFAIVGVIWLLSLVVVA
                     LPAYGAAVLAGWPRVTVVIVACAAWTASAALILIRPVEAGLARLLYRSLRRPRAAELA
                     RITPALERVCRRAGTDPHRYLLRVEEKRQVNAFALGGHVLAVTRVALELPDDMLEAVL
                     AHEVGHHRHLHPLAIILGWWYLLPFEAGDRLLRAIRRITRWLSRAFGRLNDRIGGLAG
                     GRAPDGALGMLALLLVLGVLVLVGGVLVVALGLLWLPLSLLVRVAKVLSAALSRAGEH
                     AADRHAAELGYGPGLVQVLFLFQQAEFAAPRPRGMASLLRTHPSSQARIDAIRRTVHP
                     S"
     gene            1080..1595
                     /locus_tag="VFO47_01785"
     CDS             1080..1595
                     /locus_tag="VFO47_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787464.1"
                     /translation="MPIFRLNHAVLYVRDLERSVGFYRDVLGFEAVMSMPGAAFVQAP
                     GSTNDHDLGLFQIGAAAGPSEAGRRTVGLYHLAWEVDTLAELERLAGRLAEAGALVGA
                     SDHGTTKSLYAKDPDGLEFEVAWVVPADLLDDQALAARRRIGPLDLAREKDRYGPDTK
                     GGIGISVPAGS"
     gene            complement(1824..>2389)
                     /locus_tag="VFO47_01790"
     CDS             complement(1824..>2389)
                     /locus_tag="VFO47_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET8787465.1"
                     /translation="YEERRRDLVAAEKTQAAEVERREAKKAEVLAAVDRTEALLARMR
                     AADSPAVPSRPSTPVAPPPSGGGGAAPSGAAAAAVAYARAQVGKPYCYGGSGPGCFDC
                     SGLTLMAWRQGGVSLPHSSASQYNVGRRISASELRPGDLIFYYSPISHVSIYIGGGQR
                     ISATHTGDYVRVQSLGSSIVGYVRPTG"
BASE COUNT          330 a          856 c          932 g          271 t
ORIGIN      
        1 cgcaacccga tcgtacgttc aggagggatg gacggtccgg cggatggcgt cgatgcgggc
       61 ctgggaggag ggatgggtgc ggagcaggga ggccatgccg cggggccggg gggcggcgaa
      121 ctcggcctgc tggaacagga acagcacctg gaccaggccg gggccgtagc cgagctcggc
      181 cgcgtgccgg tcggcggcgt gctcgccggc ccgcgacagc gccgccgaca gcaccttggc
      241 cacgcggacg agcaggctca gcggcagcca gagcagcccc agggcgacca ccaggacgcc
      301 acccaccagg accagcacgc caaggacgag cagcagggcg agcatgccga gggcgccgtc
      361 gggggcccgg ccgccggcca gcccgccgat ccggtcgttc agccgcccga aggcccggga
      421 cagccacctg gtgatccggc ggatggcccg cagcagccgg tcgccagcct cgaagggcag
      481 caggtaccac cagccgagga tgatcgccag gggatggagg tggcggtggt ggccgacctc
      541 gtgggccagc accgcctcca gcatgtcgtc gggcagctcg agggcgaccc gggtcacggc
      601 cagcacgtgg ccgccgaggg cgaacgcgtt gacctgccgc ttctcctcga cccggagcag
      661 gtagcggtgc gggtcggtcc cggcccggcg gcacacccgc tcgagggccg gggtgatccg
      721 ggccagctcg gccgcccgcg gccgccgcag gctccggtac agcagccggg ccagcccggc
      781 ctccaccggc cggatcagga tgagggcggc actggccgtc caggcggcgc aggccacgat
      841 cacgaccgtc acccggggcc agccggccag gacggccgcc ccgtaggcgg gcagggccac
      901 cacgaccagc gacagcagcc agatgacgcc cacgatggcg aacatcccga ccgaggtccc
      961 gctgacccgc gaccgggccg ggtccacggc ccgctcccgg gcggctgggc gctgctgcgg
     1021 cggcatgggg caacgatacg tagagtgagc ctgaggtgcg acgatcggag gaccgaccca
     1081 tgcccatctt ccggctgaac cacgccgtgc tgtatgtgcg cgacctggag cgcagcgtcg
     1141 gcttctaccg cgacgtgctc ggcttcgagg ccgtcatgtc catgcccggg gcggccttcg
     1201 tccaggcccc cggctcgacc aacgaccacg acctgggcct gttccagatc ggggccgccg
     1261 ccggcccctc cgaggccggg cggcgcaccg tcggcctgta ccacctggcc tgggaggtcg
     1321 acaccctggc cgagctggag cggctggccg gtcgcctggc cgaggccggc gccctggtcg
     1381 gcgcctccga ccacggcacc accaagagcc tgtacgccaa ggaccccgac ggcctcgagt
     1441 tcgaggtcgc ctgggtggtc ccggccgacc tgctggacga ccaggccctg gccgcccgca
     1501 ggcgcatcgg ccccctcgac ctggcccggg agaaggaccg ctacggcccc gacaccaagg
     1561 gcggcatcgg catctcggtc ccggccgggt cctagcccgg gcccggccta ggtggtcgag
     1621 gtcgggagct cggtcggcgg cgggaccacc gtgccggtgg tctccgtgtc cgaggtggtg
     1681 ggggcgatgg tggtggtcac gcctccaccg gcggtggtct gcggctcggc cgggcccata
     1741 cagaaggccc atacagaagg cccgccacgg tcggcgggcc ttctgtatgg gggaactgcg
     1801 cgcgggaggc gcggtgcggg ggtctatccg gtgggccgga cgtagccgac gatgctgctg
     1861 ccgagggact ggacacgcac gtagtcgccg gtgtgggtgg ccgagatgcg ctgaccgccg
     1921 ccgatgtaga tggacacgtg cgagatgggg ctgtagtaga agatcaggtc gccgggccgg
     1981 agctcgctgg ccgagatgcg ccggccgacg ttgtactggg aggcgctgga gtgggggagc
     2041 gagacgccgc cctgccgcca ggccatcagc gtcagcccgg agcagtcgaa gcagccgggg
     2101 cccgacccgc cgtagcagta gggcttgccg acctgggccc tggcgtaggc cacggccgcc
     2161 gcggccgccc cgctgggggc ggcgccgccg ccaccggacg gcgggggcgc gaccggggtc
     2221 gacgggcggc tggggacggc ggggctgtcg gcggcgcgca tccgggccag cagggcctcg
     2281 gtcctgtcga cggccgccag cacctcggcc ttcttggcct cgcggcgctc gacctcggcg
     2341 gcctgggtct tctcggccgc gaccaggtca cgccggcgct cctcgtagg
//
LOCUS       DASSWR010000110         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_569357,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000110 DASSWR010000000
VERSION     DASSWR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569357"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..406)
                     /locus_tag="VFO47_01795"
     CDS             complement(50..406)
                     /locus_tag="VFO47_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787466.1"
                     /translation="MDEEARRRVSLAVRAGEAVTDPADAPAAVELARLAQRRLEDRTV
                     PSALRISAGAVLAWLLLVALPVTISTGVHLAALAAGLGVGLLLFSVILLLGRRQLRLA
                     RRAERRNLRLLEGDGA"
     gene            complement(445..1026)
                     /locus_tag="VFO47_01800"
     CDS             complement(445..1026)
                     /locus_tag="VFO47_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET8787467.1"
                     /translation="MGDLVSLGVMGIRGSFSEEAALEYARDNNLGDVEIVELLTTEKV
                     LRAVEAGEVDIGIFALENSNGGVVYESVYAMAAHRFRVVDLFEIDVNHTLLVHPTLPG
                     REGIERIASHQQAIRQCRMWLRHHFPTTLVTEEPDTAEAARMLATGELPVSTAVIAAE
                     RCAELFGLRVLERRIQDLKFNFTTFVAAGPRTS"
     gene            complement(1019..1909)
                     /locus_tag="VFO47_01805"
     CDS             complement(1019..1909)
                     /locus_tag="VFO47_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787468.1"
                     /translation="MQTRASTPTDLAVPGDGIRLHARDWGGSGQAVVLLHGLASNARI
                     WDGVAPRLTGAGLRVVALDQRGHGASDQPASGYDFGTLVRDLDAALAALELERPVLVG
                     HSWGANVALQHAADRPGALAGLALVDGGLASVAAWAGATREEARRAMAPPRFAVPLAD
                     WLARAGRFDPGGPDGQPWVREYLRAGVEVDDHDVARSRFHFDNHMQVVDALWDQRPEA
                     LFPLVGCPVLLCPADDPAGGDLAGVKRDAVGRALALFPSATVTWFEDTMHDIPLQRPA
                     ELAAELARFADHAKGVAGNG"
     gene            complement(1957..2448)
                     /locus_tag="VFO47_01810"
     CDS             complement(1957..2448)
                     /locus_tag="VFO47_01810"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HET8787469.1"
                     /translation="MATTLVFLVRHAKAANRFDWTGSDRDRPLIGKGRKQADRLAGAL
                     RREEPVLVAASPWLRCRQTAAPLAAAVGLQVELDDRLGYDAPDLAAWVRTVVREHPGR
                     AVVGVSHGDLIPMYLFQTGLVDGFPEFRTGSLFRLEASRGRVTGVELVDRKALKRQLE
                     GSS"
BASE COUNT          341 a          941 c         1004 g          309 t
ORIGIN      
        1 ggcccgaggt ggccaaggcc ctgcgcgagg cgccgtaggc cgcggccggc taggcgccgt
       61 caccctccag gaggcggagg ttgcgccgct cggcacggcg ggccaggcgg agctggcggc
      121 ggccgaggag caggatcacg ctgaacagca ggaggccgac gccgaggccg gcggccaggg
      181 cggccaggtg gacgccggtg ctgatggtca ccgggagggc gaccaggagc agccaggcca
      241 gcacggcccc ggcgctgatg cggagcgcgc tcgggacggt gcggtcctcc aagcggcgct
      301 gggccaggcg ggccagctcg accgcggccg gggcgtcagc cgggtcggtc accgcctcgc
      361 cggcgcggac ggccagcgac acccgccgcc tggcctcctc atccatcggg acacccccct
      421 ggggttcccc gaacccctcc ggactcagct cgtccgtggg cccgccgcca cgaaggtggt
      481 gaagttgaac ttgaggtcct ggatgcgccg ctcaaggacc cgcaggccga acagctcggc
      541 gcagcgctcg gccgcgatca cggccgtgga caccgggagc tcgccggtgg ccagcatgcg
      601 ggccgcctcg gcggtgtcgg gctcctcggt gaccagggtg gtggggaagt ggtggcgcag
      661 ccacatgcgg cactggcgga tggcctgctg gtgggaggcg atccgctcga tgccctcccg
      721 accgggcagg gtgggatgga ccagcagggt gtggttgacg tcgatctcga acaggtccac
      781 cacccggaac cggtgggcgg ccatcgcgta gacgctctcg tagacgaccc cgccgttgga
      841 gttctccagg gcgaagatgc cgatgtcgac ctcgcccgcc tccaccgccc gcagcacctt
      901 ctcggtggtg aggagctcga cgatctcgac gtcgccgagg ttgttgtcgc gggcgtactc
      961 gagcgccgcc tcctctgaaa aagagccgcg gatgcccatg acgccgaggc tgaccagatc
     1021 acccattgcc ggccaccccc ttggcgtggt cggcgaagcg ggccagctcc gcggccagct
     1081 cggccggccg ctgcagtggg atgtcgtgca tcgtgtcctc gaaccaggtg acggtggcgg
     1141 atgggaacag ggcgagggcc cggccgacgg cgtcccgctt gaccccggcc aggtcgccgc
     1201 cggccgggtc gtcggccggg cagagcagta ccgggcagcc gaccagcggg aacagcgcct
     1261 ccggccgctg gtcccagagc gcgtcgacga cctgcatgtg gttgtcgaag tggaaccggg
     1321 agcgggccac gtcgtggtcg tcgacctcga ccccggcccg caggtactcc cggacccagg
     1381 gctggccgtc cggccccccg gggtcgaagc gcccggccct ggccagccag tcggccagcg
     1441 ggaccgcgaa ccggggcggg gccatggcgc ggcgggcctc ctcccgggtc gcgcccgccc
     1501 acgcggccac gctggccagg ccaccgtcga ccagggcgag gccggccagc gccccgggcc
     1561 ggtcggcggc gtgctggagg gcgacattcg ccccccacga gtgcccgacc agcaccggcc
     1621 gctccagctc gagggcggcc agggcggcgt cgaggtcgcg gaccagtgtc ccgaagtcgt
     1681 agccggaggc gggttggtcg gaggcgccgt ggccgcgctg gtcgagggcg accacccgca
     1741 ggccggcgcc ggtcagccgg ggcgcgaccc cgtcccagat gcgggcgttg gaggcgagcc
     1801 cgtgcaacag cacgaccgcc tgcccgctcc cgccccagtc gcgggcgtgc aggcggatcc
     1861 cgtcgccggg cacggcgagg tcggtcgggg tgcttgccct ggtctgcatg ggccgctcat
     1921 cgtacgggac ccccgtggcc tccgcctcgg cattcctcag gaggagccct ccagctgccg
     1981 cttgagcgcc ttgcggtcga ccagctcgac tcctgtgacc cggccccggc tggcctcgag
     2041 ccggaacagc gagccggtgc ggaactcggg gaacccgtcg accaggccgg tctggaacag
     2101 gtacatgggg atcaggtcgc cgtggctgac cccgaccacc gcccggccgg ggtgctcgcg
     2161 cacgaccgtc ctgacccagg cggccaggtc gggggcgtcg tagccgagcc ggtcgtccag
     2221 ctcgacctgg aggcccacgg ccgcggccag cggagcggcc gtctggcggc agcgcagcca
     2281 ggggctggcc gcgaccagca ccggctcctc ccggcgcagg gcgccggcca gccggtcggc
     2341 ctgcttgcgg cccttcccga tcaggggccg gtcgcggtcg ctgcccgtcc agtcgaaccg
     2401 gttggccgcc tttgcgtgcc tgaccaggaa caccagggtg gtcgccatgc cccagagtgt
     2461 ggcgggccgc ccggtgccag gcaaccggta cgggccgcca ggccaggcgg gcactaggcc
     2521 ggggtggcgt ggaaggtggg ggcgagggcg gggtacaggc cgcggaaccg cgcgtgaccc
     2581 tcgcggtaga cgtcc
//
LOCUS       DASSWR010000111         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_343232,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000111 DASSWR010000000
VERSION     DASSWR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343232"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFO47_01815"
     CDS             complement(<1..516)
                     /locus_tag="VFO47_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8787470.1"
                     /translation="MHPDLAFALELADLADAITVERFQADDLVVETKPDLSPVTEADR
                     VVEQVLRKRIGEERPGHSVVGEEFGADEPGSARWIIDPIDGTKNYLRGIPVWATLIAL
                     EREGRVEIGVVSAPALHRRWWGARGEGAFVDGRRIHVSRIAELSDAVLSHASFASWEE
                     HGLGEQFLTLVR"
     gene            615..824
                     /locus_tag="VFO47_01820"
     CDS             615..824
                     /locus_tag="VFO47_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HET8787471.1"
                     /translation="MADQELRGSNLEKDPAQWKTGDEPMTGAQASYLKTLSDQASEAF
                     DENLTKAEASRRIDELQERTGRGQS"
     gene            complement(888..1772)
                     /locus_tag="VFO47_01825"
     CDS             complement(888..1772)
                     /locus_tag="VFO47_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8787472.1"
                     /translation="MPLGLGSGLCWGAADFFGGIQSRHLPALTVAFWSQVAGALALAT
                     ALAIEGTPPNATGFAWGLAAGIGAGCALTLFYRGLAEGTMSIVAPISACGAIVPVAAA
                     LLTGNQPGSLAALGVLIAVIGVVLVSRPAPTAEGPGAVANRGRSRRVVAMALGAALGF
                     GLFYVLVDAGTTGSRGAPLWVIAGARTSSLAVLSTLALVGQRGWGDRRSRRPALRWPG
                     RRIGPVALIGIGDTGANLLFAYAAIGGNLAVVGVLASLYPVTTVVLARWLLGERLSGG
                     QNAGVVLALTGVGLLAAA"
     gene            complement(1810..1941)
                     /locus_tag="VFO47_01830"
     CDS             complement(1810..1941)
                     /locus_tag="VFO47_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787473.1"
                     /translation="MEVIWLVLVLLAVDLAAFLFAADTRPGLQHTPRWRDRHRATTG"
BASE COUNT          333 a          751 c          792 g          251 t
ORIGIN      
        1 gcggaccagc gtcaggaact gctcgccgag cccgtgctcc tcccacgagg cgaagctggc
       61 gtggctcagg acggcgtcgg acagctcggc gatccgtgac acgtggatgc ggcggccgtc
      121 gacgaacgcc ccctcgcccc gtgcccccca ccaccgccgg tgcagcgccg gcgccgacac
      181 caccccgatc tcgacccggc cctcgcgctc gagggcgatg agggtcgccc acaccgggat
      241 gccgcgcagg tagttcttgg tgccgtcgat cgggtcgatg atccagcggg cgctgcccgg
      301 ctcgtcggcc ccgaactcct ccccgaccac gctgtggccg ggtcgctcct cgccgatccg
      361 cttgcgcagg acctgctcca ccacccggtc ggcctcggtc accgggctca ggtcgggctt
      421 ggtctcgacg acgaggtcgt cggcctggaa gcgctcgacg gtgatggcgt cggccaggtc
      481 ggccagctcg agggcgaagg cgaggtcggg atgcataccc ggcagcctac aggggcgggc
      541 ctgtggatgg gtttcggcgg gcgtccgggg ggcatgctag cctcgaacac gagttcgatt
      601 ccaaggaggg cgacatggcc gaccaggagc ttcgtggctc caacctcgag aaggaccccg
      661 cccagtggaa gaccggggac gagccgatga ccggcgccca ggcgtcctat ctcaagaccc
      721 tctccgacca ggccagcgag gccttcgacg agaacctgac caaggccgag gcgtcccgcc
      781 ggatcgacga gctccaggag cgcaccggcc gcgggcagtc ctgaccgggg cccgtggccg
      841 gggtctgggt cggaaccggg tggggtcggg gcagggtcgg ggccgggtca ggcggcggcc
      901 agtagcccca cgccggtgag ggcgagcacg acgccggcgt tctggccgcc cgacagccgc
      961 tcgcccagca gccagcgggc caggacgacg gtggtgaccg ggtacagcga cgcgagcacg
     1021 cccacgacgg cgaggttgcc accgatggcc gcgtaggcga acagcagatt ggccccggtg
     1081 tcgccgatcc cgatcagtgc caccggcccg atccggcgcc ccggccagcg cagggccggc
     1141 cgccggctcc gccggtcccc ccagccccgc tggccgacga gcgcgagcgt cgacaggacc
     1201 gccagcgagc tggtccgggc accggcgatc acccacagcg gtgcgccacg ggatccggtc
     1261 gttccggcgt ccacgagcac gtagaacagc ccgaacccga gcgccgcccc cagcgccatc
     1321 gccaccaccc gccgcgacct cccccggttg gcaacggcac cagggccttc ggcggtaggg
     1381 gcggggcggg agacgaggac gacgccgatg acggcgatga ggacaccgag ggcggcgagg
     1441 ctgcccggct ggttgccggt gaggagggcg gcggcgaccg ggacgatggc gccgcaggcc
     1501 gagatggggg cgacgatcga catggtgccc tcggccaggc cgcggtagaa gagcgtcagg
     1561 gcgcagccgg ccccgatccc ggccgcgagg ccccaggcga acccggtcgc gttgggaggc
     1621 gttccctcga tggcgagggc ggtggcgagc gccagcgcgc ccgccacctg gctccagaag
     1681 gcgacagtca gcgccggcag gtggcgggac tggatgccgc cgaagaagtc ggcggccccc
     1741 cagcacagcc ccgaccccag cccgagcggc acgcccaggt tcaagaaccc tcgaccgggg
     1801 tgatcgggct caacccgtgg tggcgcggtg gcggtcgcgc cagcgggggg tgtgctggag
     1861 gccggggcgg gtgtcggcgg cgaacaggaa cgcggccagg tcgacggcga gcaggacgag
     1921 gacgagccag atcacttcca tggtcgctcc cggcttcttg tctggtcttg ccagcgcgat
     1981 accagtacag tgaaccttca gtacaatcta ttgtactcca ctatactgaa cttgtcgaac
     2041 aatttattga tacgagcgta ggagcgggcc gtgccggagc cggacgacgt caacgccgcc
     2101 gtggggcgca acgtgcgggg gcaccgc
//
LOCUS       DASSWR010000112         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_626080,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000112 DASSWR010000000
VERSION     DASSWR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626080"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..955
                     /locus_tag="VFO47_01835"
     CDS             50..955
                     /locus_tag="VFO47_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787474.1"
                     /translation="MTDTDVAAAETERGEAEPSGRQASLWADAWRELRRNPLFLVASA
                     LILLFGAMAVAPQLFTGADPRNCDLSRSLERPSAKHWFGFDLQGCDYYALVVYGARVS
                     IAVGLLVTLGAVAVAVVLGSIAGYYGGALDTVIARVTDIWFGLPYILAAIVILSVIGT
                     RGLLQVTLVLLVVGWPTMLRLMRSSVLSAKESDYVQAARALGASGPRVLRRHILPNAI
                     APVIVYATIFIGIMIAAEATLSFVGVGLELPAISWGLMISVAQTRVLNAPHLLLFPGL
                     FLSATVFSFILLGDALRDALDPKLR"
     gene            961..1989
                     /locus_tag="VFO47_01840"
     CDS             961..1989
                     /locus_tag="VFO47_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787475.1"
                     /translation="MTQTDDVTYRPDFGGGDHLLEVEDLAVEFRTRHGVVKAVNGISF
                     HLDQGETLAILGESGSGKSVSAQAIMGIIDSPPGFVTGGAVRFRGDDLLRLSEEDRRR
                     VRGERLAMVFQDALTALNPVFSVGFQIGEMFRVHRGASRDEARRRAVELLDRVRIPSA
                     ADRVGDYPHQFSGGMRQRVMIALALALDPDVLIADEPTTALDVTVQAQIMELLADLQS
                     ETGMGLILITHDLGVVADVADRVAVMYAGKIVETGPIGDLYARPAHPYTEGLMGSIPR
                     VDQKGQDLTPIGGAPPNLLRIPSGCPFHPRCRYRQEVCTVEEPPLYEVEGRRRSACHF
                     YQEVLDGH"
BASE COUNT          273 a          768 c          688 g          319 t
ORIGIN      
        1 aacctgctgg tggacgtgct gtatgccgtc ctcgacccga ggatccgcta tgactgacac
       61 cgacgtcgcc gcggccgaga ccgaacgcgg cgaggccgag ccctccggcc gccaggccag
      121 cctctgggcc gacgcctggc gggagctgcg ccgcaacccg ctgttcctgg tcgcctcggc
      181 gctgatcctg ctcttcgggg ccatggccgt cgccccccag ctgttcaccg gcgccgaccc
      241 gcgcaactgt gacctgtcgc ggtcgctgga gcggcccagc gccaagcatt ggttcggctt
      301 cgacctgcag ggctgcgact actacgccct ggtggtgtac ggcgcccggg tctcgatcgc
      361 cgtcgggctg ctggtcaccc tcggcgcggt cgccgtcgcc gtggtgctgg ggtcgatcgc
      421 cggctactac ggcggggcgc tcgacaccgt gatcgcccgg gtcaccgaca tctggttcgg
      481 cctgccctac atcctggccg cgatcgtcat cctcagcgtc atcggcaccc gcggcctgct
      541 ccaggtgacc ctggtgctgc tggtggtcgg ctggccgacc atgctgcgac tgatgcgctc
      601 ctcggtgctg tcggccaagg agagcgacta cgtgcaggcg gccagggccc tgggggcgag
      661 cgggccgcgg gtgctgcgcc gccacatcct gcccaacgcc atcgccccgg tgatcgtgta
      721 cgccaccatc ttcatcggca tcatgatcgc cgccgaggcg accctgtcgt tcgtcggggt
      781 cggcctggag ctgccggcca tctcctgggg cctgatgatc tcggtcgccc agacccgggt
      841 gctgaacgcc ccccacctgc tgctgttccc cgggctgttc ctcagcgcca cggtattcag
      901 cttcatcctg ctcggcgacg ccctgcgcga cgctctcgac cccaagctgc ggtgaggccg
      961 atgacccaga ccgacgacgt cacctaccgg ccggacttcg gcggcggcga ccacctgctg
     1021 gaggtggagg acctggcggt cgagttccgc acccgccacg gggtggtcaa ggccgtcaac
     1081 ggcatcagct tccacctcga ccagggcgag accctggcca tcctgggcga gtcggggtcg
     1141 ggcaagagcg tcagcgccca ggcgatcatg gggatcatcg acagcccgcc tgggttcgtc
     1201 accggcggcg cggtccgctt ccgcggcgac gacctgctca ggctgagcga ggaggaccgg
     1261 cgccgggtcc gcggcgagcg gctggccatg gtcttccagg acgccctgac cgccctcaac
     1321 ccggtgttct cggtcggttt ccagatcggc gagatgttcc gggtccaccg gggcgcctcc
     1381 agggacgaag cccggcgcag ggcggtcgag ctgctggacc gggtgcggat cccctcggcc
     1441 gccgaccggg tcggcgacta cccccaccag ttctcgggcg gcatgcgcca gcgggtgatg
     1501 attgccctgg ccctggccct ggacccggac gtgctgatcg ccgacgagcc gaccaccgcc
     1561 ctcgacgtca ccgtccaggc ccagatcatg gagctgctgg ccgacctcca gtccgagacc
     1621 ggcatgggcc tcatcctgat cacccacgac ctgggggtgg tcgccgacgt cgccgaccgg
     1681 gttgccgtca tgtacgccgg caagatcgtg gagaccggcc cgatcggcga cctctacgcc
     1741 cgccccgccc acccctacac cgaggggctg atgggctcca tcccccgggt cgaccagaag
     1801 ggccaggacc tcaccccgat cggcggggcg ccccccaacc tgctgcgcat ccccagcggc
     1861 tgcccgttcc acccgcgctg ccgctaccgg caggaggtct gcaccgtcga ggagccaccg
     1921 ctgtacgagg tcgagggccg gcggcgcagc gcctgccact tctaccagga ggtgctcgat
     1981 ggccactgag ccgccgctgc tggaggtcga gaacctggtc aagcacttcc cgatcaccac
     2041 cgggatcg
//
LOCUS       DASSWR010000113         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_343237,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000113 DASSWR010000000
VERSION     DASSWR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343237"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFO47_01845"
     CDS             <1..199
                     /locus_tag="VFO47_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide isomerase"
                     /protein_id="HET8787476.1"
                     /translation="DHTWAVDTSRVFGVPTFVTGSRAVFVRLMDRPATTTAARSTLER
                     VLALVEDWPDLNEFKATVIPR"
     gene            complement(206..1039)
                     /locus_tag="VFO47_01850"
     CDS             complement(206..1039)
                     /locus_tag="VFO47_01850"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET8787477.1"
                     /translation="MPLDALTSAEISLPATPDATQVRERIAVLSRHVDLVGLTDNHAG
                     RPRMSPLAAVALAREQGVATVVHVSCRDRNRLALRSEVVGAAALGSEGILCLYGDPVA
                     DVPRVRDYTTTRLLAEAREWAAPNDLAIGCVADPFKPDVEAELALLGRKVAAGADFVQ
                     TQMVFDPAGLTAFLEQAAEGGKLGDCRVHASVPVIRTARMVDILNQTLPCQIPERVAA
                     QIAAGGGIDLAVETAMVLAGLDRVHALHVMSWGSEEQAGQVAAAFRAARGAPPERPAR
                     A"
     gene            complement(1063..1767)
                     /locus_tag="VFO47_01855"
     CDS             complement(1063..1767)
                     /locus_tag="VFO47_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HET8787478.1"
                     /translation="MTVSSAGLRQALDQLADLRTSPVSVEDALDRVMATADALFQVDG
                     TALLLVDRDQVLRNLATSDHRAVLLEELQAEHGEGPGVDAFDDKEPVSADDLRHDDRW
                     PAFAPAAVRGGLLAVLASPIPYSDQAVGVVAVFAGEPHAWSEAEREAIVAFTELVAML
                     ILNAMEARERGRLAGELQVALDSRVVIEQAKGVLVGRHGLTTRQAFERLRRQARDQRR
                     PLAEVARGVVSAAEHR"
     gene            complement(1769..2872)
                     /locus_tag="VFO47_01860"
     CDS             complement(1769..2872)
                     /locus_tag="VFO47_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8787479.1"
                     /translation="MGPVTTMVLLAGVLAAVIAVVVGLVAGRGRRGHRPAEEAGEELF
                     RARAARFLSRRLDPEAATGLALTVALGLVFAAALGFGLVSDMVTSETGLYRLDASAAA
                     WGADNATPASTWFLGAVTWLGATVTVLAVTFALGGLEWLRRRRLAVLAFMVTVVVGQN
                     LIANTVKALVDRERPPVPHLAPSSGFSFPSGHAAAAAATWAAVVLVVGRGRPFRTKAW
                     LAAGAAAVTVAVAASRVLLGVHWLTDVIGGAALGFGWFVVCAVAFGGALLHFGAPAER
                     VEAEEARLSGDQTAASPRSQSMPDHDKPGPEQVDSRAGSLAAEPGNHAEDPGAQARAL
                     LEESEQRVEDQAARDPDDQGVIRRRSDEGVSAD"
BASE COUNT          389 a         1123 c         1158 g          346 t
ORIGIN      
        1 cgatcacacc tgggcggtcg acacctcccg ggtgttcggc gtgcccacct tcgtgaccgg
       61 cagccgggcc gtgttcgtgc gcctgatgga ccgcccggcc accaccaccg ccgcccgctc
      121 caccctggag cgggtgctgg ccctggtcga ggactggccc gacctcaacg agttcaaggc
      181 caccgtcatc ccccgctgag gcccttcagg cccgggcggg tcgctccggc ggggcgcccc
      241 gggcggcccg gaaggcggcg gcgacctggc cggcctgctc ctcggacccc caggacatga
      301 cgtgcagggc atggacccgg tcgaggccgg ccaggaccat ggccgtctcg acggccaggt
      361 cgatgccccc gccggcggcg atctgggcgg ccaccctctc cgggatctgg cagggcaggg
      421 tctggttgag gatgtcgacc atgcgggccg tgcggatcac cgggacgctg gcgtggaccc
      481 ggcagtcgcc cagcttgccg ccctcggcgg cctgctccag gaaggcggtc aggccggccg
      541 ggtcgaacac catctgggtc tggacgaagt cggccccggc ggcgaccttg cggccgagca
      601 gggccagctc ggcctcgacg tcgggcttga acgggtcggc gacgcagccg atggccaggt
      661 cgttgggcgc ggcccactcc ctggcctcgg ccagcagcct ggtggtggtg tagtcgcgca
      721 cccggggcac gtccgccacc gggtcgccat agaggcacag gatgccctcg gagccgagtg
      781 cggccgcccc gacgacctcg gagcgcaggg cgaggcggtt gcggtcgcgg caggagacgt
      841 ggaccaccgt cgccaccccc tgctcgcggg ccagggcgac cgcggccagc ggcgacatgc
      901 gcggcctgcc ggcgtggttg tcggtcagcc cgaccaggtc gacgtggcgg gacaggacgg
      961 cgatgcgctc gcgcacctgg gtcgcgtccg gggtcgcggg cagcgagatc tcggcggagg
     1021 tgagggcgtc cagcggcatg ccggaaggct agcagccgcc ggtcatcggt gctcggccgc
     1081 gctgaccacg ccccgggcga cctcggcgag cgggcggcgc tggtcccgcg cctggcggcg
     1141 cagccgctcg aacgcctgcc tggtggtgag cccgtggcgc ccgacgagga cccccttggc
     1201 ctgctcgatc accacccgcg agtcgagcgc gacctggagc tcgccggcca gccggccgcg
     1261 ctcccgggcc tccatggcgt tcaggatgag catggcgacc agctcggtga aggcgacgat
     1321 cgcctcccgc tcggcctcgc tccaggcatg gggctcgccg gcgaacaccg ccaccacccc
     1381 gaccgcctgg tcgctgtagg ggatggggct ggccagcacg gccagcagcc cgccccggac
     1441 ggcggcgggc gcgaacgccg gccagcggtc gtcgtggcgc aggtcgtcgg ccgacaccgg
     1501 ctccttgtcg tcgaaggcgt ccacgccggg gccttcgcca tgctcggcct ggagctcctc
     1561 gagcaggacg gcgcggtggt cggaggtggc caggttgcgg aggacctggt cgcggtcgac
     1621 cagcagcagg gcggtcccgt cgacctggaa cagggcgtcg gcggtggcca tgacccggtc
     1681 aagggcgtcc tcgaccgaca ccggggaggt ccggaggtcg gcgagctggt cgagggcctg
     1741 gcgcaacccc gccgaagaca ccgtcatgtc agtcggcgct gacgccctcg tcggagcggc
     1801 gccggatgac gccctggtcg tccgggtcgc gggccgcctg gtcctcgacc cgctgctcgg
     1861 actcctccag cagcgcccgg gcctgggcgc cggggtcctc ggcgtggttg cccggctcgg
     1921 cggccagcga tccggcccgg ctgtcgacct gctcggggcc cggcttgtcg tggtcgggca
     1981 tggactggct cctgggtgac gcggcggtct ggtcgccgga cagcctagcc tcctcggcct
     2041 cgacccgctc ggccggggcg ccgaagtgga gcagggcacc gccgaaggcg accgcgcaga
     2101 ccacgaacca gccgaacccg agggcggccc cgccgatcac gtcggtcagc cagtggaccc
     2161 cgagcagcac ccgcgaggcg gccacggcca cggtcacggc ggcggccccg gcggccagcc
     2221 aggccttggt ccggaacggc cggccgcggc ccacgaccag gacgaccgcc gcccaggtgg
     2281 cggccgcggc ggcggcatgc cccgagggga acgagaagcc gctggagggc gcgagatggg
     2341 gcacgggcgg ccgctcccgg tcgaccagcg ccttgaccgt gttggcgatg aggttctggc
     2401 cgaccaccac ggtcaccatg aaggcgagca cggccaggcg ccgccggcgc agccactcca
     2461 ggccccccag agcgaacgtg acggcgagca cggtgacggt cgcgcccagc caggtgaccg
     2521 cgcccaggaa ccaggtcgag gccggggtgg cgttgtcggc cccccaggcg gcggcgctgg
     2581 cgtcgaggcg gtagagcccg gtctcggagg tgaccatgtc ggacaccagc ccgaagccga
     2641 gggcggcggc gaagaccagc ccgagggcga cggtcagggc cagcccggtg gccgcctccg
     2701 ggtcgagccg ccggctcagg aaccgggccg ccctggcccg gaacagctcc tcgccggcct
     2761 cctcggccgg gcggtgaccg cggcggccgc ggccggccac cagcccgacg accacggcga
     2821 tcacggcggc cagcacgccc gccagcagga ccatggtggt gaccgggccc atgagctaga
     2881 gctcccttcg gatggcggcg ctcgcgagct ccttggcccg caggcgcacc gggcgggacc
     2941 gccaggcggt cagctcgagc tcgcgagcgt cgtcgaggtc ggccaggacg tgcttctcca
     3001 ggccgaccac gacccc
//
LOCUS       DASSWR010000114         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1105936, whole genome shotgun sequence.
ACCESSION   DASSWR010000114 DASSWR010000000
VERSION     DASSWR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105936"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFO47_01865"
     CDS             complement(<1..265)
                     /locus_tag="VFO47_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HET8787480.1"
                     /translation="MRRVAWVMVLVMAAVAAPYASAGRAAAQPTTVPFPDFDHDGFGD
                     LAVGVPGEDVGTAVDAGAVQVLYGSAGGLVSDGRALTQGAGGVP"
     gene            complement(371..1324)
                     /gene="meaB"
                     /locus_tag="VFO47_01870"
     CDS             complement(371..1324)
                     /gene="meaB"
                     /locus_tag="VFO47_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET8787481.1"
                     /translation="MNLPELLERLKAGDKRAVARLISWVEDGDREQLRDAAEALNPAT
                     GRAQVIGLTGSPGVGKSTLADALVATYRAAGLSVGVLAVDPSSPFTGGALLGDRVRMQ
                     RHALDEGVYIRSMATRGHLGGLAWATPQAVRVLDAAGCDVVLVETVGVGQAEVEVAGL
                     ADTTLVALAPGFGDAVQVAKAGILEVADVFVVNKADRDGAEVVARDLRQMLHLGEAQP
                     WQVPVVLTVAERGDGVGKLVEAIAAHRDHLASSGELDRRRRDRAAREIEEVALADLRT
                     ELGELGRGEALDTLAEQVAAGKLGPYSAADQLLAGVRGDRG"
     gene            complement(1321..>2501)
                     /locus_tag="VFO47_01875"
     CDS             complement(1321..>2501)
                     /locus_tag="VFO47_01875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET8787482.1"
                     /translation="RWASWPRHRSPAGSARTWWFPLCCARPESTVRVAAALERAGADP
                     GAVEHVVMGHVLQAGQGQITARQAAARGGVPMTVPALTVNKVCLSGLNAIATAAQYVE
                     LGQAGLLVAGGMESMSGAPYVVPKARFGARMGDAELVDAMVHDGLWDAFDDAHMGELS
                     DQVNQRLGIDRAAQDEWSARSHERAAEATKNGVLGEEIAPVVVAQRKGEVTVDQDEGI
                     RPGTTAEALGRLRPAFSPDGTITAGNASQISDGAAAVVVTSRERAAADGLPVLASVVA
                     YGTVAGPDTSLHSQPSRAIADACRRAGVAPGDLDLVEINEAFAAVALRSTRELELDEG
                     IVNVNGGAVALGHPIGASGTRVVLTLAMELRRRGGGLGAAALCGGGGQGDALLVRVEG
                     "
BASE COUNT          309 a          957 c          940 g          295 t
ORIGIN      
        1 ccggtacccc gccagcgccc tgggtcaggg ctcgcccgtc ggagaccagc ccgcccgccg
       61 aaccgtacag cacttgaacc gccccggcgt cgaccgccgt gcccacgtcc tcgccgggta
      121 cccccaccgc caggtcgccg aagccgtcgt ggtcgaaatc ggggaagggg accgtcgtcg
      181 gttgggcggc ggcccgcccg gcggaggcgt acggcgccgc caccgccgcc atcacaagca
      241 ccatgaccca cgccacgcgt cgcatctcga cctcctgatc ttcggacgct ccgacggtgg
      301 ccagcctcgc ggcgacccga cgcccgggac aggaagccga ccggcaattc cccggcaaca
      361 ggggggcgga tcagccgcgg tcgcccctga ccccggccag gagctggtcg gcggcgctgt
      421 aggggccgag cttgccggcg gcgacctgct cggccagggt gtcgagggcc tcgccgcggc
      481 cgagctcgcc cagctcggtg cggaggtcgg ccagggcgac ctcctcgatc tcgcgggcgg
      541 cacggtcgcg gcggcggcgg tccagctcgc cggaggacgc caggtggtcg cggtgggcgg
      601 cgatggcctc gaccagcttc ccgaccccgt cgccgcgctc ggccacggtc aggaccaccg
      661 ggacctgcca gggctgggcc tcccccaggt ggagcatctg gcgcaggtcg cgggcgacca
      721 cctcggcgcc gtcgcggtcg gccttgttga ccacgaacac gtcggccacc tcgaggatgc
      781 cggccttggc gacctggacc gcgtccccga atccgggggc cagggcgacc agggtggtgt
      841 cggccaggcc ggccacctcg acctcggcct ggccgacccc gacggtctcg accaggacga
      901 cgtcgcagcc ggcggcgtcc agtacccgca ccgcctgcgg ggtcgcccag gccaggccgc
      961 ccaggtggcc ccgggtcgcc atggagcgga tgtagacccc ctcgtcgagg gcgtggcgct
     1021 gcatccgcac ccggtcgccg agcagggccc cgccggtgaa cggcgaggac gggtcgacgg
     1081 ccagcacccc cacggacagt ccggcggccc ggtaggtcgc gacaagggcg tcggccaggg
     1141 tcgacttgcc gaccccgggc gacccggtga gcccgatcac ctgggcccgc ccggtggccg
     1201 ggttgagcgc ctcggcggcg tcccggagct gctcgcggtc gccgtcctcg acccaggaga
     1261 tcagccgggc cacggcccgc ttgtcgccgg ccttcagccg ttcgaggagc tcgggaaggt
     1321 tcacccctcg acccggacca gcagggcgtc gccctggccg ccgccgccgc acagggcggc
     1381 cgcgccgagc ccgccgccgc ggcggcgcag ctccatggcc agggtgagca cgacccgggt
     1441 gccgctggcc ccgatggggt ggccgagggc gaccgcgccg ccgttgacgt tgacgatgcc
     1501 ctcgtccagc tccagctcgc gggtcgagcg cagggcgacc gcggcgaagg cctcgttgat
     1561 ctcgaccagg tccaggtcgc cgggggcgac cccggcccgg cggcaggcgt cggcgatggc
     1621 ccgcgacggc tgggagtgca ggctggtgtc gggaccggcc acggtcccgt aggccacgac
     1681 cgaggcgagc accggcaggc cgtcggcggc ggcccgctcc cgggaggtga cgaccaccgc
     1741 cgccgccccg tcggagatct gggaggcgtt gccggcggtg atggtgccgt cgggtgagaa
     1801 cgccggccgg agccggccga gggcctcggc ggtggtgccg ggccggatgc cctcgtcctg
     1861 gtcgacggtg acctcgccct tgcgctgggc caccaccacc ggggcgatct cctcgcccag
     1921 gacgccgttc ttggtcgcct cggcggcccg ttcgtgggag cgggccgacc actcgtcctg
     1981 ggcggcccgg tcgatcccga ggcgctggtt gacctggtcg gacagctcgc ccatgtgggc
     2041 gtcgtcgaag gcgtcccaga gcccgtcgtg gaccatcgcg tcgaccagct cggcgtcgcc
     2101 catacgggct ccgaagcggg ccttgggcac cacgtagggg gcgccgctca tcgactccat
     2161 gcccccggcg accagcagcc cggcctggcc gagctcgacg tactgggcgg cggtggcgat
     2221 ggcgttcagc cccgacaggc agaccttgtt gacggtgaga gcgggcacgg tcatggggac
     2281 gccgccccgg gccgcggcct gcctggccgt gatctgcccc tgcccggcct ggagcacgtg
     2341 ccccatgacc acgtgctcga ccgccccggg gtcggccccg gcccgctcca gggcggccgc
     2401 gacccggacg gtagactcgg gacgagcgca gcacaggggg aaccaccatg ttcgagcgga
     2461 cccagccggt gatctgtgcc ggggccagga cgcccatcgg g
//
LOCUS       DASSWR010000115         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_795834,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000115 DASSWR010000000
VERSION     DASSWR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795834"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1354
                     /locus_tag="VFO47_01880"
     CDS             89..1354
                     /locus_tag="VFO47_01880"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET8787483.1"
                     /translation="MKIAVAGGGSTYTPELVQGFATRENRLPVDELVLFDIDADRLEV
                     VAGLAGRMLARAGWGGRLQQTGRREQAIDGADFVVVQLRVGGQAMRSVDETLPLEFGC
                     VGQETTGPGGFAKALRTVPVVLELAEEMSRRGAPGAWFVDFTNPVGIVTQALLDRGHR
                     AIGLCNSAIGFQRRFAAQLGVAPERVELEHVGLNHLTWERAVRVDGTDRLPELLERHG
                     DQLARDLGLAPALLRALGAVPSTYLRYYYATAEVLDEQRRGPSRAEQVAEIERGLLEL
                     YSDPGLDTKPELLERRGGAFYSEAAAALIATLHAGTGDVQVVDVRNDGALPGLPDDAV
                     VEVPARIDTDGAHPLPLDPLAPELLGLVQQAKAYERLAVEAAVTGDRTVALKALLANP
                     LVREYRVAAPLLDALLQASRPYLPRFFAG"
     gene            complement(1365..>2526)
                     /locus_tag="VFO47_01885"
     CDS             complement(1365..>2526)
                     /locus_tag="VFO47_01885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET8787484.1"
                     /translation="VEAVAEVVRSGRWGGFPEPGPLAAEFAGRFAAYQGSRHGVVMVN
                     GTVTMEVALKALGIGWGDEVIVPALTFAATPYAAMAAGALPVFCDVLPDTLTIDPDQV
                     EAAVTSRTRAIMPVHLGHQMADMDRLTEIARRHGLALVEDCAHAHGQRWRDRGAGSFG
                     AFGSFSHQSTKLLTAGEGGSLITNDEGLARRAHSLIDCGRAKDPEEKEFTFGANYRLG
                     ELHAALLLCALERLPGQQAQRAEAGRHFEQLAGQVPGIRLLPPDPRITRWSFYRYIVL
                     IDPEAFAGAGNRAVCQALEAEGVGAWEGYEPMSRYDLFQPALSRLPVAVEHATRLDPA
                     RMSFPVAEDAGLRRTVYLDENVFRAGRRGVEDAVEALAKVQRHADELVEAGT"
BASE COUNT          327 a          949 c          925 g          325 t
ORIGIN      
        1 gtagagtaag aaaactttcc taacctactc atggtggctg aacaatagca gccggtggag
       61 agcgagcgaa agaggggcgg ccgtgggcct gaagatcgcg gtggccggtg gcggcagcac
      121 ctacaccccc gagctggtcc agggcttcgc cacccgggag aaccgcctgc ccgtcgacga
      181 gctggtgctg ttcgacatcg acgccgaccg cctcgaggtg gtggccggcc tggccgggcg
      241 gatgctggcc cgggccggct ggggcggtcg cctgcagcag accggccggc gcgagcaggc
      301 catcgacggc gccgacttcg tggtcgtcca gctccgggtg ggcgggcagg cgatgcgcag
      361 cgtggacgag accctgccgc tggagttcgg ctgcgtcggc caggagacga ccgggccggg
      421 cggcttcgcc aaggccctgc gcaccgtccc ggtggtgctg gagctggccg aggagatgtc
      481 gcgccgtggc gcccccgggg cctggttcgt cgacttcacc aacccggtcg gcatcgtcac
      541 ccaggcgctg ctggaccggg ggcaccgcgc catcgggctg tgcaactcgg ccatcgggtt
      601 ccagcgccgc ttcgccgccc agctgggcgt ggcccccgag cgggtcgagc tcgagcacgt
      661 gggcctcaac cacctcacct gggagcgcgc cgtcagggtg gacgggaccg accggctgcc
      721 cgagctcctg gagcggcacg gcgaccagct ggcccgcgac ctgggcctgg cgccggcgct
      781 gctccgggcc ctcggggccg tcccctcgac ctacctccgc tactactacg ccaccgccga
      841 ggtgctggac gagcagcggc ggggcccctc ccgcgccgag caggtggccg agatcgagcg
      901 gggcctactt gagctgtaca gcgaccccgg gctcgacacc aagcccgagc tgctggagcg
      961 gcgcggcggg gccttctaca gcgaggccgc ggccgccctg atcgccaccc tccatgccgg
     1021 caccggcgac gtgcaggtgg tcgacgtccg caacgacggg gccctgcccg gcctgcccga
     1081 cgacgccgtg gtcgaggtcc cggcacgcat cgacaccgac ggcgcgcacc cgctgcccct
     1141 cgacccgctc gcccccgagc tcctcggcct ggtgcagcag gccaaggcct acgagcgcct
     1201 ggccgtcgag gccgcggtga ccggcgaccg caccgtcgcc ctcaaggcgc tgctcgccaa
     1261 ccccctggtc cgcgagtacc gggtggccgc gcccctgctg gacgcgctcc tccaggccag
     1321 ccggccgtac ctgccgaggt tcttcgccgg ctgacgggcc ggcctcaggt gccggcctcg
     1381 accagttcgt cggcgtgccg ctggaccttg gccagggcct cgaccgcgtc ctcgaccccc
     1441 cgccgtccgg cccggaagac gttctcgtcc aggtacacgg tccggcgcag gccggcgtct
     1501 tcggccaccg ggaacgacat ccgggccggg tcgaggcgcg tggcgtgctc gacggcgacc
     1561 ggcaggcgcg acagggccgg ctggaacagg tcgtagcggc tcatcggctc gtagccctcc
     1621 caggcgccga ccccctcggc ctccagcgcc tggcagaccg cccggttgcc cgccccggcg
     1681 aaggcctccg ggtcgatcag cacgatgtag cggtagaagc tccagcgggt gatgcgggga
     1741 tcgggcggga gcagccggat ccccggcacc tgcccggcca gctgctcgaa gtgccggccc
     1801 gcctcggccc gttgcgcctg ctggccgggg agccgctcca gggcacacag gagcagggcc
     1861 gcgtgcagct cgcccagccg gtagttggcg ccgaaggtga actccttctc ctccgggtcc
     1921 ttggcccgcc cgcagtcgat cagcgagtgg gcccgccggg ccagcccctc gtcgttggtg
     1981 atcaggctgc cgccctcccc ggcggtgagg agcttggtgg actggtggct gaaggagccg
     2041 aacgcgccga acgacccggc cccccggtcg cgccagcgct ggccgtgggc atgggcgcag
     2101 tcctccacca gcgccagccc gtggcgccgc gcgatctccg tgagccggtc catgtcggcc
     2161 atctggtggc cgaggtgcac cggcatgatg gccctggtcc gggaggtgac ggccgcctcg
     2221 acctggtcgg ggtcgatggt cagggtgtcg gggaggacgt cgcagaacac cggcagcgcg
     2281 ccggcggcca tggccgcgta cggggtggcc gcgaaggtca gggccgggac gatcacctcg
     2341 tcgccccagc cgatcccgag ggccttgagc gcgacctcca tggtgacggt gccgttgacc
     2401 atcaccacac cgtgccggga gccctggtag gcggcgaacc gcccggcgaa ctcggcggcc
     2461 agcggtcccg gctcggggaa tccgccccag cggccgctcc gcaccacctc ggcgaccgcc
     2521 tcgacg
//
LOCUS       DASSWR010000116         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_230790,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000116 DASSWR010000000
VERSION     DASSWR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230790"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VFO47_01890"
     CDS             <1..1189
                     /locus_tag="VFO47_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787485.1"
                     /translation="EQYLARLGPRIELDGETHQVATDRVTIEPFDLRQPVRHALVIDR
                     LAYWYDHPREWLKKIALMDRAYLLNNPFTFQAMEKHAGYCAAIRLGFDVPATWLLPHK
                     VPPDNERFAPTASRYNRAFDLPAVGEQVGYPMYMKPFHGGGWVNVYRIADPYELQAAY
                     DASGRELMHVQAGVEGYDVFTRGLAIGPQTMVTHYDPTKPLHLRYQVDHDFLTPEAGN
                     EIVTFGSSIGAFFRWEFNSFEVIVRDGACYPIDFANATPDMAIISLHYYFPWAITALA
                     KWSLYCAVTGRAMRLDTEIGRWYTVADDPSLGWEEKLAAYRGLVDDYYDADRFTEFCA
                     THLAHAEECMAEYISSEQFDAHLVATIQHAFPPHEHEQFVAHYRGLLGAWVNDQRAAA
                     GQP"
     gene            complement(1247..>2127)
                     /locus_tag="VFO47_01895"
     CDS             complement(1247..>2127)
                     /locus_tag="VFO47_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787486.1"
                     /translation="GTVGVPLLLLLAGVVLLAARFWPDLATWTRLSLVGLVAAALWGA
                     GALVPEQAAPALWRLRGVLWLGSSAAVAFLAALFGTPVLELDSEAIALGAGLAAATHA
                     GALWWRRPGPLQQLACLAGLAVAAGAGVALAGGNEAAVGLSIWTVGVLWVLAGWRGLL
                     PPSVVALVAGAIVVLQGAQATAVGWEGAGLVFGLASAAGLLVAGTTGRRLALAAVGIM
                     GVVLFLPATVIYFFAGTVGVPLLLLLAGVVLLAVTLVLLRWRQPWVRATPRTPAGERP
                     EQRSSPSDAPTGLPAA"
BASE COUNT          351 a          848 c          689 g          239 t
ORIGIN      
        1 cgagcagtac ctggcccggc tcggcccccg catcgagctc gacggcgaga cccaccaggt
       61 ggccaccgac cgggtcacca tcgagccctt cgacctgcgc cagccggtcc gccacgccct
      121 ggtcatcgac cgcctcgcct actggtacga ccacccccgg gagtggctca agaagatcgc
      181 cctgatggac cgggcctacc tgctcaacaa ccccttcacc ttccaggcga tggagaagca
      241 cgccggctac tgcgcggcca tccggctcgg gttcgacgtc cccgcgacct ggctgctgcc
      301 gcacaaggtc ccgcccgaca acgagcgctt cgccccgacc gcgtcgcgct acaaccgggc
      361 cttcgacctg ccggcggtgg gggagcaggt cggctacccg atgtacatga agccgttcca
      421 cggcggcggc tgggtcaacg tctaccgcat cgccgacccc tatgagctcc aggccgccta
      481 cgacgcctcg ggccgcgagc tgatgcacgt ccaggccggg gtcgagggat acgacgtctt
      541 cacccggggc ctggccatcg gcccccagac gatggtcacc cactacgacc cgaccaagcc
      601 cctgcacctc cgctaccagg tcgaccacga cttcctgacc cccgaggcgg gcaacgagat
      661 cgtcaccttc gggtccagca tcggagcgtt cttccgctgg gagttcaaca gcttcgaggt
      721 gatcgtccgc gacggagcgt gctacccgat cgacttcgcc aacgccaccc cggacatggc
      781 catcatctcg ctgcactact acttcccgtg ggcgatcacg gccctggcca agtggtcgct
      841 gtactgcgcg gtcaccgggc gggcgatgcg cctggacacc gagatcggcc gctggtacac
      901 ggtcgccgac gacccgtccc ttggctggga ggagaagctg gccgcctacc gggggctggt
      961 cgacgactac tacgacgccg accgcttcac cgagttctgc gccacccacc tggcccacgc
     1021 cgaggagtgc atggccgagt acatctccag cgagcagttc gacgcccacc tggtggccac
     1081 catccagcac gcattcccgc cccatgagca cgagcagttc gtcgcccact accgcggcct
     1141 gctcggcgcc tgggtcaacg accagcgggc ggccgccggc caaccatgac tggtgcatct
     1201 gctgacgcgt cgcacaaggc gagcggtgtc aggatggagc agcgagtcag gccgccggta
     1261 gcccggtcgg ggcgtccgac gggctggacc gctgctcggg ccgctcgcca gcaggcgtgc
     1321 gtggcgttgc ccggacccag ggttgccgcc agcgcagcag caccagggtg acggccagca
     1381 gcaccacacc ggccagcagg agcagcagcg ggaccccgac ggtgccggcg aagaagtaga
     1441 tgaccgtggc gggcaggaac aggaccacgc ccatgatccc gaccgccgcc aacgccaagc
     1501 ggcgaccggt cgtccccgcc accagcaacc cagccgcgct ggccagcccg aagaccaggc
     1561 cggcgccctc ccagcccacc gcggtggcct gcgccccctg cagcaccacg atggccccgg
     1621 cgaccagcgc gaccacgctg ggggggagca gcccccgcca gccggccagc acccacagga
     1681 cccccaccgt ccagatgctc agcccgaccg cggcctcgtt cccgccggcc agggccaccc
     1741 cggccccggc cgccaccgcc agcccggcca ggcaggccag ctgctgcagc gggccgggcc
     1801 gccgccacca caacgccccg gcatgggtgg cggccgccag cccggccccc agggctatgg
     1861 cctcgctgtc cagctccagc accggggtgc cgaagagggc ggcgaggaag gcgaccgcgg
     1921 ccgaggatcc cagccacagc accccccgga gccgccacag cgctggggcg gcctgctcgg
     1981 ggaccagggc gccggctccc cacagggccg ccgcgaccag tccgaccagc gacagccggg
     2041 tccaggtggc caggtccggc cagaaccggg cggccagcag caccacaccg gccagcagga
     2101 gcagcagcgg gaccccgacg gtgccgg
//
LOCUS       DASSWR010000117         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1219831, whole genome shotgun sequence.
ACCESSION   DASSWR010000117 DASSWR010000000
VERSION     DASSWR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219831"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..624)
                     /gene="folK"
                     /locus_tag="VFO47_01900"
     CDS             complement(130..624)
                     /gene="folK"
                     /locus_tag="VFO47_01900"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET8787487.1"
                     /translation="MTRAHVGLGSNLGDRMAILDTAVRVLDADEQTHVMAVSRVYETA
                     PVGGPEQDPYLNAVAVLETDRSPHELLELLLATEDGLGRVRTERWGPRTADLDLLLYD
                     GPPVDSPELTVPHPRARERAFVLVPLCDADPFATFPDGELAAAALARLGPVTGVVPVG
                     ELQP"
     gene            complement(621..1004)
                     /gene="folB"
                     /locus_tag="VFO47_01905"
     CDS             complement(621..1004)
                     /gene="folB"
                     /locus_tag="VFO47_01905"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET8787488.1"
                     /translation="MTGDRILLSGLRVHGRHGVLPHEAQLGQVFVVDLELDVDLAAAG
                     RTDDLERTVDYGSLAGRVAEVVGGRPRRLLEAVAEDVAQLVLADERVRQVRVRVTKPQ
                     APLPVDARVAVEITRDRETTGEGRP"
     gene            complement(1001..1402)
                     /locus_tag="VFO47_01910"
     CDS             complement(1001..1402)
                     /locus_tag="VFO47_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8787489.1"
                     /translation="MNPVDVKAVTEANEEFYAAFEALDMDRMEACWRHDEDVRCIHPG
                     WDVMAGWPQVSRSWVAIFANSGYIQFFLTDVRVHVDADTAWVTCAENILSGGAGGPDM
                     EDAKVLATNVFRRDGDGWRMVLHHGSPVLRP"
     gene            complement(1399..2187)
                     /gene="folP"
                     /locus_tag="VFO47_01915"
     CDS             complement(1399..2187)
                     /gene="folP"
                     /locus_tag="VFO47_01915"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET8787490.1"
                     /translation="MGVVNVTPDSFSDGGLFLDPEAAVAHGLALAAEGADLLDVGGES
                     TRPGAVDVPEGVELDRVLPVVEELAAKAGVPVSVDTRKSGVAAAAVAAGAAMVNDVSA
                     GRHDPDLLGVVAEAKVPLVLMHMLGTPATMQDDPRYDDVVAEVEAFLAERCLAAEFAG
                     VDHEALVVDPGIGFGKRDRDNYALLDNLARLTRLGHPVLVGTSRKAFIGRALDRPADQ
                     RVEGTAATVVWAVERGARIVRVHDVAPLVPSVRMTEAMLRWGAT"
     gene            complement(2357..>2500)
                     /locus_tag="VFO47_01920"
     CDS             complement(2357..>2500)
                     /locus_tag="VFO47_01920"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET8787491.1"
                     /translation="AEHLCMSMRGVRKPGAVTVTSAVRGSFRDQLSTRMEAMNLLGVT
                     RLG"
BASE COUNT          333 a          947 c          914 g          306 t
ORIGIN      
        1 cgtgcccggc cagggccacg gccagctgcc cggtgccgcc accggcgtcg accaccgtgg
       61 ccggcggggc cggaagggca tccagcacct gcccgagcag cagctcgaac ccgaggcggc
      121 ccctgcccat cacggctgca gctccccgac cgggacgacc ccggtgacgg ggccgaggcg
      181 ggccagggcg gccgcggcca gctcgccgtc cgggaaggtg gcgaacgggt cggcgtcgca
      241 caggggcacc agcacgaacg cccgctcccg ggcccgcggg tggggcacgg tcagctcggg
      301 cgaatcgacc ggcgggccgt cgtagagcag caggtccagg tcggcggtgc gcggccccca
      361 gcgctcggtt cggacccggc ccaggccgtc ctcggtggcc agcagcagct ccagcagctc
      421 gtgcgggctg cggtcggtct ccagcacggc gaccgcgttg aggtagggat cctgctcggg
      481 cccgccgacg ggggccgtct cgtacacccg ggacacggcc atgacgtggg tctgctcgtc
      541 ggcgtcgagg acccgcacgg ccgtgtcgag gatggccatg cggtcgccca ggttggagcc
      601 gagcccaacg tgcgcccgtg tcacggccgt ccctcccccg tcgtctcgcg gtcccgcgtg
      661 atctccaccg ccacccgggc gtcgaccggc agcggcgcct gcggcttggt gacgcggacc
      721 cggacctggc ggacccgctc gtcggccagg accagctggg ccacgtcctc ggccaccgcc
      781 tccagcagcc gccgggggcg gccgccgacc acctcggcca cccggccggc caggctaccg
      841 tagtccaccg tccgctcgag gtcgtcggtc cgcccggccg cggccaggtc gacgtccagc
      901 tccaggtcga ccacgaacac ctggccgagc tgggcctcgt ggggcagcac cccgtggcgg
      961 ccgtggaccc gcagcccgct cagcaggatc cggtcgccgg tcatgggcgc agcaccggcg
     1021 agccgtggtg caggaccatc cgccagccgt cgccgtcgcg gcggaacacg ttggtggcca
     1081 gcaccttggc gtcctccatg tcggggccgc cggcaccgcc cgagaggatg ttctcggcgc
     1141 aggtgaccca ggcggtgtcg gcgtcgacgt ggacgcggac gtcggtgagg aagaactgga
     1201 tgtagccgga gttggcgaag atggccaccc aggaccggct gacctggggc cagccggcca
     1261 tcacgtccca gcccgggtgg atgcagcgga cgtcctcgtc gtgccgccag cacgcctcca
     1321 tgcggtccat gtccagggcc tcgaaggcgg cgtagaactc ctcgttggcc tcggtcacgg
     1381 ccttgacgtc caccgggttc acgtggcccc ccagcgcagc atcgcctcgg tcatgcgcac
     1441 ggacgggacc agcggggcca cgtcgtggac gcggacgatc cgcgccccgc gctccaccgc
     1501 ccacaccacc gtggccgccg tgccctcgac ccgctggtcg gcggggcggt ccagggcccg
     1561 gccgatgaac gccttgcggc tggtgccgac cagcaccggg tggcccaggc gggtgagccg
     1621 ggccaggttg tcgagcaggg cgtagttgtc gcggtcgcgc ttgccgaacc cgatccccgg
     1681 gtcgacgacc agggcctcgt ggtcgacgcc ggcgaactcg gcggccaggc agcgctcggc
     1741 caggaacgcc tcgacctcgg ccacgacgtc gtcgtagcgg gggtcgtcct gcatggtggc
     1801 cggggtgccg agcatgtgca tcagcaccag cgggaccttg gcctcggcga ccacgccgag
     1861 caggtcgggg tcgtggcggc cggcgctgac gtcgttgacc atggccgctc cggcggccac
     1921 ggcggccgcg gccacccccg acttcctggt gtcgaccgac accggcaccc cggccttggc
     1981 cgccagctcc tcgacgactg ggaggacccg gtccagctcg accccctcgg gcacgtcgac
     2041 ggccccgggc cgggtcgact cgccgcccac gtcgagcagg tcggccccct cggcggccag
     2101 ggccagcccg tgggccacgg ccgcctccgg gtccaggaac agccccccgt ccgagaatga
     2161 gtccggggtg acattcacca cgcccatgac cagacaccgc tgcccggtcg gcagcacccg
     2221 gtcatcgacc cgcaggtcat gcggtgcgag cggggcaaca gacacaagac aacctccggg
     2281 agtcggcccg gtcatcttac ggcgtccacg gatcggggga cgcgggtcgg ggtggcgcag
     2341 actccacccg gatgggctac cccaggcggg tgacgcccag gaggttcatg gcctccatgc
     2401 gggttgacag ctggtcgcgg aacgagccgc gcactgccga ggtcaccgtg accgcgccgg
     2461 gcttgcggac gcctcgcatg ctcatgcaga ggtgctcggc
//
LOCUS       DASSWR010000118         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_796021,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000118 DASSWR010000000
VERSION     DASSWR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796021"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFO47_01925"
     CDS             complement(<1..906)
                     /locus_tag="VFO47_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lytic transglycosylase/C40 family
                     peptidase"
                     /protein_id="HET8787492.1"
                     /translation="MGKLLFGVFATVFLVLGLMAVGLSTSLIGGGAPSASAQEDIPPP
                     LLPVYQTAADSCPGLPWSVLAAIGKVETDHGRLQAPGVTSGANFAGAAGPMQIGIGGK
                     AGNTWAAYAVDADGGGADVYNPIDAIFTAANYLCQNGANRGADVAGAVFAYNHADWYV
                     TKVLAIASTYAASQGLPPGAPAEEVAAAAVQYAYDKLGQPYKWGATGERGFYDCSGLM
                     LRAYQAGGITLPRTSREQWTAGARVWNVADLQPGDLVFYAYNTADPSSIHHVGIYIGA
                     GNMIDAPYTGANVRITPFLRGDFIGA"
     gene            1307..2380
                     /locus_tag="VFO47_01930"
     CDS             1307..2380
                     /locus_tag="VFO47_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET8787493.1"
                     /translation="MPEPPPEPARDHPPGHGHPRPEASRDLVDGRPERADRAGRLVGV
                     TSPASGRSTATAADFTAERYLRGQAAPPRDGWRRFIYWLTGGRINFGPSPAELAERHL
                     IARAKAPVAGCRSIAVISRKGGVGKTTTTLMLGHTFASLRGDRVVALDGNPDAGSLGY
                     RVRRETAATVTNLLADEQEITRYADIRAYTNQAPTRLEVVASDDDPRITTALGEEDYR
                     RAIGLLERHYNLILLDTGTGVLESATKGILQLAEQIVVVMSPSLDSARAASSTLDWLN
                     ENGHSELVRNAIAVVNAVREEGLVEVDKIEEHFQGRCRAVVQVPWDPHLSAGAETQVD
                     RLRPVTRRAYLEVAAAVADGFTA"
     gene            2400..2699
                     /locus_tag="VFO47_01935"
     CDS             2400..2699
                     /locus_tag="VFO47_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HET8787494.1"
                     /translation="MLLRMMVLAQDVSFTPDFDAIPGSGPIQQLINGIGAFALLLSLV
                     GIIIGGAMWGVGSLSSNYHQAAVGKRATLYSVVGAVIVGAAAALVNWAFSLGQTA"
     gene            2793..>2978
                     /locus_tag="VFO47_01940"
     CDS             2793..>2978
                     /locus_tag="VFO47_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787495.1"
                     /translation="MAVRDQRARVLYRLPEQRRFGARGMPGLLTMALLAVVLFLGGLV
                     VGRASMTRDQADVAAPTA"
BASE COUNT          417 a         1049 c         1091 g          421 t
ORIGIN      
        1 ggcgccgatg aagtcgccgc gcaggaacgg ggtgatgcgg acgttggccc cggtgtaggg
       61 ggcgtcgatc atgttgccgg cgccgatgta gatgccgacg tggtggatgg aggaggggtc
      121 ggcggtgttg taggcgtaga agaccaggtc gccgggctgc aggtcggcca cgttccagac
      181 ccgggcgccg gccgtccact gctcgcggga ggtgcggggc agggtgatcc cgcccgcctg
      241 gtaggcgcgc agcatcaggc ccgagcagtc gtagaagccc cgctcgccgg tggcgcccca
      301 cttgtagggc tggccgagct tgtcgtaggc gtactggacg gccgcggccg ccacctcctc
      361 ggccggggcg cccgggggca ggccctggct ggccgcgtag gtcgaggcga tggccaggac
      421 cttggtcacg taccagtcgg cgtggttgta ggcgaacacg gccccggcca cgtcggcgcc
      481 cctgttggcg ccgttctggc agaggtagtt ggccgcggtg aagatcgcgt cgatcgggtt
      541 gtagacgtcg gccccgccgc cgtcggcgtc gacggcgtag gcggcccagg tgttgccggc
      601 cttgccgccg atgccgatct gcatcgggcc ggcggccccg gcgaagttgg cccccgaggt
      661 caccccgggc gcctggagcc ggccgtggtc ggtctcgacc ttgccgatgg cggccagcac
      721 cgaccagggc agccccgggc agctgtcggc ggccgtctgg tagacgggca gcagcggcgg
      781 cgggatgtcc tcctgggcgc tggcgctcgg cgccccgccg ccgatgagcg aggtcgacag
      841 tcccaccgcc atgagcccga ggaccaggaa gacggtggcg aagacgccga acaggagttt
      901 gcccatcagg tggccaatcg gtggtatttt aggatagtcg agtcggttga caggttcggc
      961 tcatcagggt ggcggagctg ccgcatcgat ccaccgaccg cgggccgtgc atcggcgggt
     1021 ccgattcccg ctggtcgact gccttggtgt cattgcacat cagtgtaatc tgttggtaat
     1081 cacagaggga cagatcctcc caaatacgca tccgacagca cgagaatctg gagaccgacc
     1141 gggggtcgtt gtgagccagc agcagaggcc cgcctggacc gggggttccg acgaggccct
     1201 ggtcccgctc ggccgtggcg acgaaccggc tccgaccacc gatcgtgacc ggccgtccga
     1261 gcctgccccg gcgcccgagc ccgaccgggc ccccgagcgt cccaggatgc cagagccgcc
     1321 cccggagccg gccagggacc acccgcccgg ccacggccac ccacggccgg aggcgtcccg
     1381 cgacctcgtc gacgggcgcc ccgagcgggc cgaccgggcc gggcgcctgg tcggggtgac
     1441 cagcccggcc agcggccggt cgaccgccac cgcggccgac ttcaccgccg agcgctacct
     1501 gcgcggccag gccgcaccgc cccgggacgg ctggcggcgc ttcatctact ggctgaccgg
     1561 cggccgcatc aacttcggcc ccagcccggc cgagctggcc gagcgccacc tgatcgccag
     1621 ggccaaggcg cccgtggccg gctgccgctc gatcgccgtc atcagccgca agggcggggt
     1681 cggcaagacc accaccaccc tcatgctcgg ccacaccttc gcctcgctgc ggggcgaccg
     1741 ggtggtcgcc ctcgacggca accccgacgc cgggtcgctc ggctaccggg tccggcggga
     1801 gacggcggcc acggtcacca acctgctcgc cgacgagcag gagatcacgc gctacgccga
     1861 catccgcgcc tacaccaacc aggccccgac ccgcctggag gtggtcgcct ccgacgacga
     1921 cccgcgcatc accaccgcgc tgggtgagga ggactaccgc cgcgccatcg ggctgctgga
     1981 acgccactac aacctcatcc tgctcgacac cggcaccggt gtcttggaat ccgctacaaa
     2041 gggaatcctc cagttggccg agcagatcgt cgtcgtcatg tcgccgagcc tggactcggc
     2101 cagggcggcc agctcgacac tggactggct gaacgagaac ggccactccg agctggtccg
     2161 caacgccatc gcggtcgtca acgcggtgcg ggaggagggc ctggtcgagg tggacaagat
     2221 cgaggagcac ttccaggggc gctgccgggc cgtggtccag gtgccgtggg acccccatct
     2281 gagcgcaggc gccgagaccc aggtcgaccg tttgcgcccc gttaccagac gcgcgtactt
     2341 ggaggtagcc gccgccgtgg ccgatggatt cacggcatga ccatcggagg gaactaccga
     2401 tgctgttgcg catgatggtc ctcgcgcaag atgtcagctt caccccggac ttcgacgcca
     2461 tccccggttc ggggccgatc cagcagctga tcaacgggat cggggccttc gccctgctgc
     2521 tgtcgttggt gggcatcatc atcggcgggg ccatgtgggg cgtcggctcg ctgtcgtcca
     2581 actaccacca ggcggccgtg ggcaagcggg ccacgctgta ctcggtggtc ggcgccgtga
     2641 tcgtcggggc ggccgcggcg ttggtcaact gggcgttctc gctcgggcag accgcctagg
     2701 gcgccgatgg cgacttcccc gacgccggag gaccggcggg tgtgccggcc ctcgtcggcg
     2761 ttggcggatc cggcggcggg ggggtgaggg ccatggccgt gcgcgaccag cgggcccggg
     2821 tcctgtaccg gctccccgag cagcggcgct tcggggcccg cggcatgccg ggcctgctga
     2881 ccatggccct gctggccgtg gtgctgttcc tgggcggcct ggtcgtcggc cgcgcctcca
     2941 tgaccaggga ccaggcggac gtcgccgccc ccacggcg
//
LOCUS       DASSWR010000119         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_569788,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000119 DASSWR010000000
VERSION     DASSWR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569788"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..296)
                     /locus_tag="VFO47_01945"
     CDS             complement(93..296)
                     /locus_tag="VFO47_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787496.1"
                     /translation="MVTGDELKARRHAREIVADMRWCGMTVPPLHVRMANEFQDLVRS
                     GDYAAWVAANQTPALLSRRSYPR"
     gene            complement(283..903)
                     /locus_tag="VFO47_01950"
     CDS             complement(283..903)
                     /locus_tag="VFO47_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8787497.1"
                     /translation="MRTCDVMSTPVVTVSPDAPLKDVAATLVEHGINAVPVVDASDRL
                     IGIVSEADLLSLETGLGRGPAPAGPRTAGEVMRQSVYTLTGDTAATAAARLMVRHRLK
                     SVPVVAGERVVGMVARRDLIRLIARSDDDIQADLDRRLKEEIQVLQRLAVGVAGGVVT
                     IDAAASPLARRLLEGLARDIPGVVEVRTTQRGADGAEPPRGTDGDR"
     gene            complement(914..1051)
                     /locus_tag="VFO47_01955"
     CDS             complement(914..1051)
                     /locus_tag="VFO47_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787498.1"
                     /translation="MVTCPRCGNDADYLADDTFVLIRCEFCGDDIDATDLVPPGDAQA
                     A"
     gene            complement(1146..2213)
                     /locus_tag="VFO47_01960"
     CDS             complement(1146..2213)
                     /locus_tag="VFO47_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HET8787499.1"
                     /translation="MTVDLRTTYLGLELRSPLVASPSPLTGELDGLRRLEAAGVGAVV
                     LPSLFEEQLTHDQLELDRLLETTSDHTGEALSYFPDLQDYNTGPWTYLDHIAQAKRAV
                     SVPVIASLNGITPGGWVGHAKRMRDAGADALELNLYTVATDPRVGAAELEARYLDLIA
                     DMRAAVSIPLAVKLSPFFTALANFAVRVDEAGADGLVLFNRFYQPDLDLDSRDVVPRL
                     VLSNSEELRLPLRWIAILFGRVGISLAATTGVHTGLDAAKVLLAGANVAMMTSALLRH
                     GPEHLQTVEAELRTVMAEHDLDSVAQLRGSMSHASMPDPAGFERANYMRTLMSWSSRA
                     EVSPGQAAGDEPAPPAGRAAR"
     gene            complement(2210..>4187)
                     /locus_tag="VFO47_01965"
     CDS             complement(2210..>4187)
                     /locus_tag="VFO47_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8787500.1"
                     /translation="LDLWVVDAARVAREVGMGHRINTVMQPCFFALSGVLPRDEAIAA
                     IKAWIERAFGRRGEEVVARNFAAVDRALDGLRQVQVPASATATRHRRPTVSEQAPDFV
                     KRVTARMMEGNGDLLPVSALPVDGAFPTGTARWEKRSLAAEIPIWDPSICIDCGKCAI
                     VCPHSTIRMKVFAPEALEGAPDGFEAKDFRSRDLPGHKLTIQVAPDDCTGCGVCVEVC
                     PAHSKEEVKHKAINMTPVDEFLERERQRWDFFEAVPELDCERMAPDTVKGSQVRQPLF
                     EFSGACAGCGETQYLKLISQLFGDRLLVANATGCSSIYGGNLPTTPWTTNAEGQGPAW
                     ANSLFEDNAEFGLGIRLAADRQAARARQLVERLAPAVGEDLARAILAADQHGEAEVAA
                     QRERVAELRRRLAGVDGGAARELEGLAGELVRRSVWIVGGDGWAYDIGSAGVDAVLAS
                     GQDVNLLVLDTEVYSNTGGQASKATPRGAVAKFAARGKQTTKKDLGMLAMAYGNVYVA
                     HVAVGASDMQTVKALLEAEAWPGPSLVIAYSTCIAHGFDMTGSMSHMREAVRSGHWPL
                     YRYHPEAERPFHLDSKPPTVPLREFAGSEARFAMLARTAPEEFERLMDLAEADARERW
                     RYYEQLAGLSRTAPHLPPRPVTPAGTGPTTEEAP"
BASE COUNT          564 a         1497 c         1539 g          587 t
ORIGIN      
        1 gcgccgggga ggacgcccgc gtggtccatc aacatgacgg cccacaccac caggatcgat
       61 cccatgaaca cggccagcag cacctcggac gatcagcgtg gataggatct tcggctgagc
      121 agggcgggcg tctggttcgc cgcgacccag gcggcgtagt cgccgctgcg caccaggtcc
      181 tggaactcgt tggccatgcg gacatgcagc ggtggcacgg tcatgccgca ccagcgcatg
      241 tcggcgacga tctcgcgggc gtgccgccgc gccttcagct cgtcaccggt caccatcggt
      301 gcccctgggt ggctcggcgc catcggctcc ccgctgggtg gtgcgcacct cgaccacgcc
      361 ggggatgtca cgagcgagtc cctcgaggag ccggcgggcg agcgggctgg cggcagcgtc
      421 gatggtgacg accccgccgg cgacgcccac ggccagccgc tggagcacct ggatctcctc
      481 cttgagccgg cggtcgaggt cggcctggat gtcgtcgtcg ctgcgggcga tcaggcggat
      541 caggtcgcgc cgggcgacca tgccgaccac ccgttccccg gccaccaccg ggacggactt
      601 gaggcggtgg cgcaccatga gccgggcggc ggcggtggcg gcggtgtcgc cggtgagggt
      661 gtagaccgac tggcgcatca cctcgccggc cgtgcggggc ccagccgggg cggggcctcg
      721 gccgagcccg gtctccagcg acagcaggtc ggcctcggac acgatcccga tgagccggtc
      781 gctggcatcg accaccggca cggcgttgat cccatgctcg accagggtcg cggccacgtc
      841 cttgagggga gcatccggcg acacggtcac caccggagtg ctcatcacgt cgcaggtgcg
      901 catgccgtcc tcctcaggcg gcctgcgcgt cgcccggcgg gacgaggtcg gtggcgtcga
      961 tgtcgtcgcc gcagaactca cagcggatca ggacgaaggt gtcatcggcg aggtagtcgg
     1021 cgtcgtttcc gcaccgtggg caggtgacca tgggtcctct ccttcgacgg cggtgctgct
     1081 gcaccggcct tggacccagc atcagcccac ggccctggcc gcacctacgg ccgaaggtcc
     1141 cgctcctagc gggccgcccg gcccgccggg ggtgccggtt cgtcgccggc cgcctgaccg
     1201 gggctgacct cggcccggct cgaccaggac atgagggtgc gcatgtagtt ggcccgctcg
     1261 aacccggccg ggtcgggcat ggaggcatgg ctcatgctcc cgcggagctg ggccaccgag
     1321 tcgaggtcat gctcggccat gacggtccgc agctccgcct ccaccgtctg aagatgctcg
     1381 ggcccgtggc gcagcagggc cgaggtcatc atggccacgt tcgccccggc cagcaggacc
     1441 ttggccgcgt ccaggccggt gtggaccccg gtggtggcgg ccagcgagat cccgacccgg
     1501 ccgaacagga tggcgatcca gcgcaggggc aggcgcagct cctcggagtt cgacagcacc
     1561 agccgcggca ccacgtcgcg gctgtccagg tccaggtcgg gctggtagaa gcggttgaac
     1621 aggaccagcc cgtcggcccc ggcctcgtcg acccgcacgg cgaagttggc cagggcggtg
     1681 aagaacgggc tcagcttgac ggccagcggg atcgacaccg ccgcccgcat gtcggcgatc
     1741 aggtccaggt agcgggcctc cagctcggcc gcgccgaccc gcgggtcggt ggccaccgtg
     1801 tagaggttca gctccagggc gtcggcgccg gcgtcgcgca tccgcttggc gtgcccgacc
     1861 cagccgcccg gggtgatgcc gttgaggctg gcgatcaccg ggacgctcac cgcccgcttg
     1921 gcctgcgcga tgtggtccag gtaggtccag ggcccggtgt tgtagtcctg gaggtcgggg
     1981 aagtaggaga gggcctcgcc ggtgtggtcc gaggtcgtct ccagcagccg gtccagctcc
     2041 agctggtcgt gggtgagctg ctcctcgaac agcgagggca gcaccaccgc cccgaccccg
     2101 gccgcctcca gccgccgcag cccgtccagc tccccggtca gcggcgaggg cgaggcgacc
     2161 agcgggctcc gcagctccag ccccagatag gtggtgcgca ggtcgacggt cacggcgcct
     2221 cctcagtggt cggcccggtg ccggctgggg tgaccggccg cgggggcaga tggggggcgg
     2281 tgcgggacag gccggcgagc tgctcgtagt agcgccagcg ctcgcgggcg tccgcctcgg
     2341 ccaggtccat cagccgctcg aactcctcgg gggcggtgcg ggccagcatg gcgaagcggg
     2401 cctcgctgcc cgcgaactcc cgcagcggca ccgtcggcgg cttggagtcg agatggaacg
     2461 ggcgctccgc ctcagggtgg tagcggtaca gcggccagtg gcccgaccgc acggcctcgc
     2521 gcatgtggct catcgacccg gtcatgtcga acccgtgggc gatgcaggtc gaataggcga
     2581 tcaccagcga cgggccgggc caggcctcgg cctccagcag ggccttgacg gtctgcatgt
     2641 cgctggcccc gacggccacg tgggccacat agacgttgcc gtaggccatg gccagcatgc
     2701 ccaggtcctt cttggtggtc tgcttgccgc gggcggcgaa cttggccacc gcgccccgcg
     2761 gtgtagcctt ggacgcctgc cctccggtgt tggagtagac ctcggtgtcc agcaccagca
     2821 ggttcacgtc ctggccggag gccaggaccg cgtccacccc ggccgagccg atgtcgtagg
     2881 cccagccgtc cccgccgacg atccagacgc tgcgccggac gagctccccg gccaggccct
     2941 cgagctcgcg cgccgccccc ccgtccacgc cggcgagccg gcggcgcagc tcggccaccc
     3001 gctcgcgctg ggcggcgacc tcggcctcgc cgtgctggtc ggcggccagg atggcccggg
     3061 cgaggtcctc ccccaccgcc ggcgccagcc gctcgaccag ctggcgggcc cgggccgcct
     3121 gccggtcggc ggccaagcgg atgccgaggc cgaactcggc gttgtcctcg aacagcgagt
     3181 tggcccaggc cggcccctgt ccctcggcgt tggtcgtcca gggggtggtt ggcaggttgc
     3241 cgccgtagat ggaggagcag ccggtggcgt tggcgaccag cagccggtca ccgaacagct
     3301 ggctgatcag cttgaggtac tgggtctcgc cgcagccggc gcaggccccg gagaactcga
     3361 acagcggctg gcggacctgg gagcccttga ccgtgtcggg ggccatccgc tcgcagtcca
     3421 gctcggggac ggcctcgaag aagtcccagc gttgccgctc gcgctccaag aactcgtcga
     3481 ccggggtcat gttgatcgcc ttgtgcttga cctcctcctt ggagtgggcc gggcagacct
     3541 cgacgcagac cccgcagccg gtgcagtcgt cgggcgcgac ctggatggtg agcttgtgtc
     3601 cgggcaggtc ccgggagcgg aagtccttgg cctcgaaccc gtcgggggcg ccctccagag
     3661 cctcgggggc gaacaccttc atgcggatgg tcgagtgcgg gcacacgatg gcgcacttgc
     3721 cgcagtcgat gcagatcgac gggtcccaga tggggatctc ggcggccagg gagcgcttct
     3781 cccacctggc cgtgccggtc gggaaggcgc cgtccacggg cagggcgctg accggcagca
     3841 ggtcgccgtt gccttccatc atccgggccg tgacccgctt gacgaagtct ggggcctgct
     3901 ccgagacggt cggccggcgg tgacgggtgg cggttgccga cgccggcacc tggacctgac
     3961 gcagcccgtc cagagcccgg tccacggcgg cgaagttgcg ggccaccacc tcctcccccc
     4021 ggcggccgaa cgccctctcg atccaggcct tgatcgccgc gatggcctcg tcccggggca
     4081 ggacgccgga cagggcgaag aagcacggct gcatcacggt gttgatccgg tggcccatgc
     4141 cgacctcccg ggccacccgg gcggcgtcca ccacccacag gtccagg
//
LOCUS       DASSWR010000120         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_541864,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000120 DASSWR010000000
VERSION     DASSWR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541864"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFO47_01970"
     CDS             complement(<1..855)
                     /locus_tag="VFO47_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET8787501.1"
                     /translation="MMADQPPPQTPPRQVEPATLPPVVSLAEAVHLDPIPARALLGTK
                     AASLARLAGAGFPVPAGVVVTAAAAADWDQAWTRLRSAVAGFGGRDRRFAVRSSATAE
                     DLAGASFAGQYETVLDVGLDELPDAVRQVVDSAASARVAAYRQAHPQAVPADSSGSTG
                     GMAVLVQVMVPAEAAGVVFTANPLTGDRDEVVISAVPGLGERLVAGQATGDQWIVRHA
                     HARRTRATEQAISTDQARQIATLARRVHAHLGSPQDLEWAIAIDPSDRDGADGVEGGL
                     WLLQARPMT"
     gene            complement(852..1724)
                     /locus_tag="VFO47_01975"
     CDS             complement(852..1724)
                     /locus_tag="VFO47_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8787502.1"
                     /translation="MPATSRQQVLAALAAAIHASDRHGPRQGQDGDGLAGLSMDELAT
                     RAGVSRATLYRLFGSQQDLLHELGLQPPPTVRSRVLDTALELVGRHGLAELSMDELAA
                     TAGVSRATLYRLVPGKEALFAELVRSFSPFEPIAAVLQTMGERPPAEVVPAIAQAMAA
                     AMDGHIGLLLQLLFELSRSDPDHRGGADASQDAAQAMRTLPLVSRYLDQQMAAGRLRR
                     MDPVLAMQALAGPIVMHLLMQASAESQSGAGDGVALPPLEVVVDELAGMWLRAMTPGD
                     GDHQQPTSNSREQA"
     gene            1870..2232
                     /locus_tag="VFO47_01980"
     CDS             1870..2232
                     /locus_tag="VFO47_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8787503.1"
                     /translation="MATATAADPATTAAACCAPLGAPSLTDAEAEATARLFKALADPH
                     RVRIVNLLATSPDPVCVCEFTGPLGLSQPTVSHHLKKLVAAGLLEREQRGTWAYYSLR
                     PDALGGLAAALDLQGASR"
     gene            2229..>2531
                     /locus_tag="VFO47_01985"
     CDS             2229..>2531
                     /locus_tag="VFO47_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite S-adenosylmethyltransferase"
                     /protein_id="HET8787504.1"
                     /translation="MSTFQTGQDALREQVRDRYAAAALKVTRGQSDCGCGQPADCGCD
                     SGCCGGAATADEPGFGAELYAALDRDELPDSAVLASLGCGNPTAVAELHAGETVLDL"
BASE COUNT          340 a          897 c          961 g          333 t
ORIGIN      
        1 cgtcatcggc cgggcctgca gcagccacaa cccgccctcg accccgtcgg cgccgtcgcg
       61 gtcgctgggg tcgatggcga tggcccattc caggtcctgg gggctgccga ggtgggcgtg
      121 gacgcggcgg gccagggtgg cgatctggcg ggcctggtcg gtgctgatgg cctgctcggt
      181 ggctcgtgtg cgcctggcgt gggcgtggcg gacgatccac tggtcgccgg tggcctggcc
      241 ggcgaccagc cgctcgccca ggccgggcac ggcgctgatg acgacctcgt cgcggtcgcc
      301 ggtcagcggg ttggcggtga agacgacccc ggccgcctcg gctgggacca tcacctggac
      361 cagcaccgcc atcccgccgg tagacccgga cgagtcggct gggactgcct gggggtgagc
      421 ctggcggtag gcggcgacac gcgccgaggc ggccgagtcc accacctgcc gcaccgcgtc
      481 gggcagctcg tccaggccga cgtccaggac ggtctcgtac tggccggcaa acgacgcgcc
      541 ggccaggtcc tcggcggtgg cggaggagcg gaccgcgaac cgccggtccc gaccgccgaa
      601 cccggcgacg gcgctgcgca gccgcgtcca cgcctggtcc cagtcggcgg cggcggccgc
      661 ggtcaccacc accccggccg gaaccgggaa gcccgctccg gccaggcgcg ccaggctggc
      721 cgccttggtg cccagcaggg cccgcgccgg gatggggtca aggtggacgg cctcggcgag
      781 gctgacgacc ggggggagcg tcgcaggctc gacctggcgg ggcggcgtct ggggcggtgg
      841 ctggtcggcc atcatgcctg ctcccgtgaa ttcgacgtgg gctgctggtg gtcgccgtcg
      901 ccgggggtca tggcccgcag ccacatcccg gccagctcgt cgacgaccac ctccaggggc
      961 ggcagggcca ccccatcgcc ggcgccggac tgggattcgg ccgaggcctg catgagcagg
     1021 tgcatcacga tcgggccggc cagggcctgc atcgccagga ccgggtccat ccggcgcagg
     1081 cggccggcgg ccatctgctg gtccaggtag cggctcacca gcggcagggt gcgcatcgcc
     1141 tgggccgcat cctggctggc gtcggcgccg ccacggtggt cgggatcgct gcgggacaac
     1201 tcgaacagca gctgcagcag caggccgatg tggccgtcca tggcggcggc catcgcttgg
     1261 gcgatggcgg ggacgacctc ggccgggggc cgctcgccca tggtctgcag caccgcggcg
     1321 atcggctcga agggcgagaa gcttcggacc aactcggcga acagcgcctc cttgccggga
     1381 accagccggt acagggtggc gcgggagacg ccggcggtgg cggcgagctc gtccatggac
     1441 agctcggcca ggccgtggcg gccgaccagc tccagggcgg tgtcgaggac gcggctgcgc
     1501 accgtcggcg gcggctggag gccgagctca tggagcaggt cctgttggct gccgaacagc
     1561 cggtacaggg tggcgcggga gacgcccgct cgggtggcca gctcgtccat cgacagcccg
     1621 gccagcccgt ctccgtcctg gccttgtctc ggcccgtgcc ggtcgctggc gtggatggcg
     1681 gcggcgaggg ccgccaagac ctgctgcctg gaggttgctg gcatgggcat gagatgatac
     1741 tagacgagat gtatcgactc gtccagtatc taaggatcag agttttccag ctcagcagtt
     1801 gattgacaga tgtcgatgta tgatggcacc ctcgggatcg acgttcatcg atacgagagg
     1861 acctgttgga tggcgaccgc caccgcagcc gacccggcca ccacggcagc cgcctgctgc
     1921 gccccgctgg gagcaccgtc gctgacggac gccgaggccg aggccacggc ccggctgttc
     1981 aaggccctgg ccgaccccca ccgggtcagg atcgtcaacc tgctggccac cagccccgac
     2041 ccggtctgcg tgtgcgagtt caccgggccg ctggggctca gccagccgac cgtcagccac
     2101 cacctcaaga agctggtcgc ggccgggctc ctggagcgcg agcagcgcgg gacctgggcc
     2161 tactactcgc tgcgacctga cgccctgggc ggtctggccg ccgccctcga cctccaagga
     2221 gccagccgat gagcaccttt cagaccggtc aggacgcgct gcgtgagcag gtccgggacc
     2281 gctacgcggc cgccgccctc aaggtcaccc gcggccagag cgactgcggc tgtgggcagc
     2341 cggccgactg cggctgcgac agcggctgct gtgggggcgc cgcgaccgcc gacgagcccg
     2401 gcttcggggc cgagctgtac gccgccctgg accgtgacga gctgcccgac agcgccgtgc
     2461 tggccagcct gggctgcggc aaccccacgg cggtggccga gctgcacgcc ggcgaaacgg
     2521 tcctggacct g
//
LOCUS       DASSWR010000121         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_965385,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000121 DASSWR010000000
VERSION     DASSWR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965385"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFO47_01990"
     CDS             <1..380
                     /locus_tag="VFO47_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004600302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET8787505.1"
                     /translation="PAKGEKVKHRPLPALAVTRTVSAERSAAPASSSRRSTVTRSAAA
                     PAGGGVWDRLAQCESGGNWGINTGNGYSGGLQFAPGTWRANGGSGSAHNASRAEQIRV
                     AERVRASQGWGAWPACSAKLGLR"
     gene            482..2035
                     /locus_tag="VFO47_01995"
     CDS             482..2035
                     /locus_tag="VFO47_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8787506.1"
                     /translation="MTQQASLLASRVGAGRADGGRRHRSVDPAHTGDVVAEVLLASPA
                     TFVDACRAARQAQPGWAATPAPVRGRVVKHLGRLVEENAEALARLVTREIGKPLAEAR
                     GEVTEIVDTCDFFAGEGRRLYGQTVPSEMPDKQLFTFREPVGVAAVITAGNFPVAVPS
                     WYLVPALLCGNAVVWKPAECAPATAEALAQLCWHAGLPDGVLNLVQADGPSTFEGLGA
                     ALEAGLVDKVGFTGSTEVGRRIGELCGRHLQVPCLELGGKNPLVVMPDADLDLAVEGA
                     LFSGFGTAGQRCTSLGTVIAHREVHDEFLARFAAAVEGAAVGDPFQDVLYGPMIDQRF
                     CDRFLGWLDLVQPHHRVHGSTGTGRITAANPRRGFVGDPEDGLYCHPTIVSRVTAGDE
                     LYRRETFGPIVGVAACGSLEEAVELANGHGYGLSAAIYTHDPATAFRFRAGVGSGMVS
                     VNNSTSGAEAHLPFGGNGRSGNGSRQSGIWVLDQFTRWQALNWDYAGRLQKAQMDVAE
                     PTPDRDFRI"
     gene            2032..>2924
                     /locus_tag="VFO47_02000"
     CDS             2032..>2924
                     /locus_tag="VFO47_02000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016825292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8787507.1"
                     /translation="MSGPLEGLVVADLSRVLAGPFATMLLGDLGADVIKVEHPGGGDE
                     TRAWGPPWQGGHSTYYLAVNRNKRSVALDLKTDAGRRAARALAGRADVLVENFKAGAL
                     ERLGLGFEEVARDNPGLVWCSVSGFGRGAGADLPGYDFLVQAMSGLMSITGPAGGEPT
                     KVGVALVDVLTGLYAFGGVLAALGERERTGRGQRVEVSLLGSALASLVNQASSYLCTG
                     RPPEAMGNRHPSIAPYETLATADRPLVVAVGNDGQFARLCRVLGLPEAAADPRFATNA
                     DRVTNREALVGLLEEALAARGA"
BASE COUNT          355 a         1121 c         1067 g          381 t
ORIGIN      
        1 ttcccgccaa gggcgagaag gtcaagcacc gtcccctgcc ggcgctggcc gtgacccgca
       61 ccgtctccgc ggagcggtcg gccgctcccg cctccagctc ccggcgctcg accgtgaccc
      121 ggtcggccgc ggctccggcc ggcggcggcg tctgggaccg cctggcccag tgcgagtccg
      181 gcggcaactg gggcatcaac accggcaacg gctactccgg cggcctccag ttcgctcccg
      241 gcacctggcg tgccaacggc ggcagcggct cggcccacaa cgccagccgc gccgagcaga
      301 tccgcgtcgc cgagcgcgtc cgggcctccc agggctgggg cgcctggcct gcctgctcgg
      361 ccaagctcgg ccttcgctga tcctcagcta gcaacaccac acggcaacat cgtggggaac
      421 ggtgcggcgc gtggtccttt gggctgcgcg ccgcgccgcg ttcgtgtact acggttgaac
      481 catgactcag caggcgtcgc tgctcgcctc ccgcgtcggc gcggggcggg ccgacggcgg
      541 gcggcggcac cggtcggtcg accccgccca caccggcgac gtggtcgccg aggtcctgct
      601 ggcctccccg gccaccttcg tcgacgcctg ccgggcggcc cgccaggccc agccgggctg
      661 ggcggccacg ccggccccgg tccggggccg ggtcgtcaag cacctgggcc gcctggtcga
      721 ggagaacgcc gaggccctgg cccgcctcgt cacccgcgag atcggcaagc cgctggccga
      781 ggcgcgcggc gaggtcaccg agatcgtcga cacctgcgac ttcttcgccg gcgagggacg
      841 gcggctctac ggccagacgg tcccgagcga gatgcccgac aagcagctgt tcaccttccg
      901 cgagccggtc ggggtggccg ccgtgatcac cgccggcaac ttcccggtgg cggtgccctc
      961 ctggtacctg gtcccggcgc tgctctgcgg caacgcggtc gtctggaagc cggccgagtg
     1021 cgccccggcc accgccgagg ccctggccca gctctgctgg cacgccggcc tgcccgacgg
     1081 cgtcctcaac ctcgtccagg ccgacgggcc gtccaccttc gaggggctgg gcgccgccct
     1141 ggaagccggc ctggtcgaca aggtcggctt caccgggtcg accgaggtgg gccggcgcat
     1201 cggcgagctc tgcggccgcc acctgcaggt gccctgcctg gagctgggcg gcaagaaccc
     1261 gctggtggtg atgcccgacg ccgacctcga cctggccgtc gagggggcgc tgttcagcgg
     1321 cttcggcacc gccggccagc gctgcacctc gctgggcacg gtgatcgccc accgcgaggt
     1381 ccacgacgag ttcctggccc ggttcgcggc cgcggtggag ggggccgccg tcggcgaccc
     1441 gttccaggac gtgctctacg ggcccatgat cgaccagcgc ttctgcgacc ggttcctggg
     1501 ctggctggac ctggtccagc cccaccaccg ggtccacggc tcgaccggga cagggcggat
     1561 caccgccgcc aacccgcgcc ggggcttcgt cggcgacccc gaggacggtc tctactgcca
     1621 cccgacgatc gtctccaggg tgaccgccgg cgacgagctg taccggcgcg agaccttcgg
     1681 cccgatcgtc ggggtggccg cctgcgggtc ccttgaggag gcggtggagc tggccaacgg
     1741 ccacggctac ggcctgtcgg cggcgatcta cacccacgac ccggcgaccg ccttccgctt
     1801 ccgcgccggc gtcgggtcgg ggatggtcag cgtcaacaac tcgacctcgg gggccgaggc
     1861 ccacctgccg ttcgggggca acggccgctc cggcaacggc agccgccagt ccggcatctg
     1921 ggtcctggac cagttcaccc gctggcaggc gctcaactgg gactacgccg gccggttgca
     1981 gaaggcccag atggacgttg ccgagcccac ccccgaccgg gacttccgaa tatgagcggt
     2041 cccctggagg ggctggtcgt ggccgacctc tccagggtgc tggccgggcc gttcgcgacc
     2101 atgctcctgg gcgacctggg ggccgacgtg atcaaggtcg agcaccccgg cggcggggac
     2161 gagacccgcg cctgggggcc gccctggcag ggcgggcaca gcacctacta cctggccgtc
     2221 aaccgcaaca agcgcagcgt cgccctcgac ctcaagaccg acgccggccg gcgggccgcc
     2281 cgggccctgg ccggccgggc cgacgtgctg gtcgagaact tcaaggcggg cgcgctggag
     2341 cggctcgggc tcggcttcga ggaggtggcg cgggacaacc cgggcctggt ctggtgctcc
     2401 gtctccgggt tcggacgggg cgcgggggcg gacctgcccg gctacgactt cctggtccag
     2461 gccatgagcg ggctgatgag catcaccggc ccggccgggg gcgagccgac caaggtcggg
     2521 gtcgccctgg tcgacgtgct caccgggctg tacgccttcg gcggcgtcct ggccgccctt
     2581 ggcgagcggg agcggaccgg gcgcgggcag cgggtcgagg tcagcctgct cggctcggcc
     2641 ctggccagcc tggtcaacca ggcgtcctcg tacctctgca ccggccggcc gcccgaggcc
     2701 atgggcaacc gccaccccag catcgccccg tacgagaccc tggccaccgc cgaccggccc
     2761 ctggtggtcg ccgtgggcaa cgacggccag ttcgcccgcc tctgccgggt tctgggtctg
     2821 cccgaggcgg ccgccgaccc gcggttcgcc accaacgccg accgggtgac caaccgcgag
     2881 gccctggtcg gcctgctgga ggaggccctg gccgcccgcg gggc
//
LOCUS       DASSWR010000122         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_852509,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000122 DASSWR010000000
VERSION     DASSWR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852509"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFO47_02005"
     CDS             <1..324
                     /locus_tag="VFO47_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787508.1"
                     /translation="AADALRAQADGDAAKAEMSRGADQGEVVSLPERFAVSPGHGRFY
                     PVTPSRYNDERPYVEPGDLLGEVRNGAASIPVRSPFRGWVMAHLAWEGELVTPGQILL
                     SLRPF"
     gene            370..1485
                     /locus_tag="VFO47_02010"
     CDS             370..1485
                     /locus_tag="VFO47_02010"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET8787509.1"
                     /translation="MPAEIVGLGVAVPPYVLTNADLEELVETNDQWITERTGVKERRV
                     AGRDDSTALLGKRAAEQAMKVAGIGVDEIDLVMVATATPDYTLPSAACILQLELGMTR
                     GAAMDVIAGCSGFVYALATASQFVDSGAAETALVVGSETLSRISDYTDRGTCILFGDG
                     AGAVVLRRGEAGVRAFSLGADGARSRLLMTPAGGSRFPANARTVAERRHYIVMEGREV
                     FKGAVTAMAASSLEAIERSGLTPDDIDLVVPHQANQRIIEATARRLKVPMTNVVMNVA
                     RYGNTSAASIPIAMAEAWEQGQIRPGQRLLLTAFGAGLAWGSVVVDWTLPNPAEPAPE
                     EPTQPALPIVRALLDGTLWDGVDMLPPLRPAVPEAAL"
     gene            1482..>2079
                     /locus_tag="VFO47_02015"
     CDS             1482..>2079
                     /locus_tag="VFO47_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8787510.1"
                     /translation="MSGLEGRTALVTGASGAIGRACAVALATRGARIVVAYGSDEQGA
                     QETADLVKAAGAEPVVAQADLTDPGAAKALVAAAGDAGVDILVNNAGLTRDGLVLRMR
                     DEDFTGVLEVNLVAAFRCTREALRGMLRRRWGRVISISSVVGLVGNPGQANYSASKAG
                     LIGLTMSVAREVAGRGITVNAVAPGYIPSKLTDAMSEEA"
BASE COUNT          277 a          762 c          776 g          264 t
ORIGIN      
        1 gccgccgacg ccctgcgcgc ccaggccgac ggcgacgcgg ccaaggcgga gatgtccagg
       61 ggcgcggacc agggcgaggt ggtgtcgctc cccgagcggt tcgccgtctc acccggccac
      121 gggcgcttct accccgtcac cccttcccga tacaacgacg agcggccgta cgtcgagccg
      181 ggcgacctgc tcggcgaggt ccgcaacggc gccgccagca tccctgtccg cagccccttc
      241 cgcggctggg tcatggccca tctcgcctgg gagggcgagc tggtcacccc cggccagatc
      301 ctgctcagcc tccggccctt ctgaccgtcc cccgaccgcc gatcccccac gccggagaaa
      361 ggagccgtcg tgcccgccga gatcgttgga ctcggggtgg ccgtgccacc ctacgtcctg
      421 accaacgcgg acctggagga gctggtcgag accaacgacc agtggatcac cgagcgcacc
      481 ggggtcaagg agcgccgggt cgcgggcagg gacgactcga cggcgctgct cggcaagcgg
      541 gccgccgagc aggccatgaa ggtggccggc atcggcgtcg atgagatcga cctggtgatg
      601 gtggccacgg ccacgcccga ctacacgctg ccctcggccg cctgcatcct ccagctcgag
      661 ctgggcatga ccaggggcgc ggccatggac gtgatcgccg gctgctcggg gttcgtgtac
      721 gccctggcca ccgcgtcgca gttcgtggac tcgggcgcgg ccgagacggc cctggtggtc
      781 ggctcggaga cgctgtcgcg gatctccgac tacaccgacc ggggcacctg catcctgttc
      841 ggtgacgggg ccggtgccgt ggtgctgcgc cggggcgagg ccggggtgcg ggcgttctcg
      901 ctgggggccg acggggcgcg gtcgcggctg ctcatgaccc cggcgggcgg gtcgcggttc
      961 ccagccaacg cccggaccgt ggccgagcgg cggcactaca tcgtcatgga gggccgcgag
     1021 gtcttcaagg gcgcggtgac ggccatggcc gcctccagcc tcgaggcgat cgagcgctcc
     1081 ggcctgaccc cggacgacat cgacctggtc gtcccccacc aggcgaacca gcggatcatc
     1141 gaggcgaccg cccggcgcct caaggtgccc atgaccaacg tggtcatgaa cgtcgcccgc
     1201 tacggcaaca cctccgccgc ctcgatcccc atcgccatgg ccgaggcctg ggagcagggg
     1261 cagatccggc ccgggcagcg gctgctgctg acggcgttcg gggccgggct ggcctggggc
     1321 tcggtggtgg tcgactggac cctccccaac ccggccgagc cggcgccgga ggagcccacc
     1381 cagccggccc tgccgatcgt ccgggccctg ctcgacggca ccctgtggga cggcgtggac
     1441 atgctgcccc ccctgcgccc ggcagtcccg gaggccgccc tatgagcggc ctcgaaggcc
     1501 ggacggcgct ggtcaccggg gcgtcggggg cgatcgggcg ggcgtgcgcg gtggcgctgg
     1561 ccacccgagg ggcgaggatc gtcgtcgcct acggctcgga cgagcagggg gcgcaggaga
     1621 cggccgacct ggtgaaggcg gccggggccg agccggtggt cgcccaggcc gacctgaccg
     1681 acccgggggc ggccaaggcg ctggtggcgg cggccggcga cgctggggtc gacatcctgg
     1741 tcaacaacgc cgggctgacc cgcgacgggc tggtgctgcg gatgcgcgac gaggacttca
     1801 ccggcgtgct cgaggtcaac ctggtcgccg ccttccgctg cacccgggag gccctgcggg
     1861 gcatgctgcg gcggcgctgg ggccgggtga tctcgatctc cagcgtggtc gggctggtcg
     1921 gcaaccccgg ccaggcgaac tactccgcct ccaaggccgg cctcatcggg ctcaccatga
     1981 gcgtggccag ggaggtggcc ggccggggca tcaccgtcaa cgccgtcgcc cccggctaca
     2041 tcccctccaa gctcaccgac gccatgagcg aggaggcca
//
LOCUS       DASSWR010000123         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_654854,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000123 DASSWR010000000
VERSION     DASSWR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654854"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFO47_02020"
     CDS             complement(<1..385)
                     /locus_tag="VFO47_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET8787511.1"
                     /translation="MARTERRLEVTDGAVLAALAHPLRVALLYQLNALGSRTASQCAK
                     ALGETPANCSYHLRQLAKVGLVVREEGGDRRERPWRSVYTGLGLHPPVDDADPGVVTA
                     ARATRASLLNLAIQEHARLARQYLSL"
     gene            complement(461..1339)
                     /locus_tag="VFO47_02025"
     CDS             complement(461..1339)
                     /locus_tag="VFO47_02025"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787512.1"
                     /translation="MPVAAVLGWPLAHTLSPALHQAAYRAAGLDGWTYEARPTRPEDL
                     AAALDEVRSGGLAGVNLTTPHKQPAAALVDVLERPADRLGAVNTVFVRDGRLVGGNTD
                     VAGAVWALGSQLGLRDGRWAGTGPAVLLGTGGVAVAVAFAWSRLARARRHQGLPVAPL
                     LVVGRDPGRAAAVAGLAGPGAQAVRWAGAAELVAGADVVLQATTLTARGEPVPGQDRL
                     RPGVRVLDCNYGPASQGLVTAARSAGAAAAADGLGMLCAQAAGAVVKIARVRPDLDAM
                     AAAVGLPWPVPGSPPI"
     gene            complement(1342..2382)
                     /gene="mltG"
                     /locus_tag="VFO47_02030"
     CDS             complement(1342..2382)
                     /gene="mltG"
                     /locus_tag="VFO47_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET8787513.1"
                     /translation="MSRVERRLRKRRRRRGVLVMGMLLALVGLVVFQALQWSAAREPK
                     PVPPGQEVTITVRAGEGSVEIGRKLREAGVVDSVNRFRDAAEERALDGVLKPGTYKLE
                     TGMDVDAVLEILAKGPSTGIPVTIPEGFTVAQIAAKLAATEQFTKDEVEKALESKDLI
                     ATLRPKGEDSLEGLLFPDTYGIEPDDTAETVIQEMLSQLEVVLSRYQLSTAPQGLTPY
                     QLLIVASMIEREAKVDADRPKIAAVIYNRLAAGKRLEIDATVEYAVGHGNLTARDLRS
                     SSPYNTYTRLGLPPTPIAAPGEAAIKAALPPADGDWIYYVLATKEGEHAFTESYQEFL
                     KLKAEAKAKGLL"
BASE COUNT          283 a          897 c          934 g          328 t
ORIGIN      
        1 cgaggctgag gtactgccgg gccaggcggg cgtgctcctg gatcgcgagg ttgagcagcg
       61 acgccctggt cgcccgggcc gcggtcacca ccccggggtc ggcgtcgtcg accggcgggt
      121 gcagtcccag cccggtgtag accgaccgcc agggccgctc gcgtcggtcg ccgccctcct
      181 cccggaccac caggcccacc ttggccagct ggcgcaggtg atagctgcag ttggccggcg
      241 tctcccccag cgccttggcg cactggctgg ccgtgcgcga cccgagcgcg ttgagctggt
      301 acagcagcgc cacccggagc gggtgggcca gggcggccag caccgccccg tcggtgacct
      361 ccagccgccg ctcggtcctc gccaccgcgc tcccctccga tctgaaagag ttctttcaga
      421 ttcagggaac cacggcatgg gcgaacctgc aacagttctt tcagatcgga ggggaaccgg
      481 gaaccggcca ggggaggccg acggcggcgg ccatggcgtc gaggtcgggg cggacccggg
      541 cgatcttgac cacggccccg gcggcctggg cgcagagcat gccgaggccg tcggcggccg
      601 cggccgcccc ggccgacctg gccgcggtga ccaggccctg gctggccggg ccgtagttgc
      661 agtcgagcac gcggacgccg gggcggagcc ggtcctggcc gggcacgggc tcgcccctgg
      721 ccgtcagggt ggtcgcctgg aggaccacgt cggcgccggc gaccagctcg gcggcgccgg
      781 cccagcgcac cgcctgggcg ccggggccgg ccagcccggc cacggcggcc gcccgtccgg
      841 ggtcgcggcc cacgaccagc agcggggcca cgggcaggcc ctggtggcgg cgggcccggg
      901 ccaggcggct ccaggcgaac gccacggcca cggccacgcc gccggtgccg agcagcaccg
      961 ccggcccggt gccggcccag cggccgtcgc gcaggccgag ctggctgccc agcgcccaca
     1021 ccgccccggc cacgtcggtg ttgccgccga ccagccggcc gtcgcgcacg aacacggtgt
     1081 tgaccgcccc gagccggtcg gccggccgct ccaggacgtc gaccagggcg gccgccggct
     1141 gcttgtgcgg ggtggtcagg ttgaccccgg cgagcccgcc cgaccggacc tcgtccagcg
     1201 ccgcggccag gtcctcgggg cgggtcgggc gggcctcgta ggtccagccg tccagcccgg
     1261 cggcgcggta ggcggcctgg tgcagggccg gcgacagggt gtgggcgagc ggccagccga
     1321 gcacggcggc gacgggcacg gctacagcag gcccttggcc ttggcctcgg ccttgagctt
     1381 gaggaactcc tggtagctct cggtgaaggc gtgctcgccc tccttggtgg cgagcacgta
     1441 gtagatccag tcgccgtcgg ccggggggag ggcggccttg atggcggcct cgccgggggc
     1501 ggcgatcggg gtcgggggca gcccgaggcg ggtgtaggtg ttgtagggcg agctggagcg
     1561 gaggtccctg gcggtcaggt tgccgtggcc gaccgcgtac tccacggtgg cgtcgatctc
     1621 cagccgcttg ccggcggcca gccggttgta gatgacggcg gcgatcttgg gccggtcggc
     1681 gtcgaccttg gcctcccgct cgatcatgct ggccacgatc agcagctggt agggggtgag
     1741 gccctggggg gcggtggaga gctggtagcg ggacagcacg acctcgagct ggctgagcat
     1801 ctcctggatc accgtctcgg cggtgtcgtc gggctcgatg ccgtaggtgt cggggaacag
     1861 caggccctcc agcgagtcct cgcccttggg ccggagggtc gcgatcaggt ccttgctctc
     1921 gagcgccttc tccacctcgt ccttggtgaa ctgctcggtg gcggcgagct tggcggcgat
     1981 ctgggccacc gtgaagccct cggggatggt gaccgggatc ccggtgctgg ggcccttggc
     2041 caggatctcg agcacggcgt cgacgtccat gccggtctcc agcttgtagg tgccgggctt
     2101 gagcacgccg tcgagggcgc gctcctcggc cgcgtcgcgg aagcggttga cgctgtccac
     2161 caccccggcc tcgcggagct tccggccgat ctcgacgctg ccctcgccgg ccctgacggt
     2221 gatggtcacc tcctgcccgg gggggaccgg cttgggctcc cgggccgcgc tccactgcag
     2281 ggcctggaac acgaccagcc ccaccagggc cagcagcatc cccatgacca gcacgccccg
     2341 gcgccgccgg cgcttgcgca gccgccgctc gacccgactc atcggggcac ccctttgggg
     2401 ttccccgaac ccctccggct catcggccgc tctcccccgc cc
//
LOCUS       DASSWR010000124         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1191113, whole genome shotgun sequence.
ACCESSION   DASSWR010000124 DASSWR010000000
VERSION     DASSWR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191113"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFO47_02035"
     CDS             complement(<1..657)
                     /locus_tag="VFO47_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET8787514.1"
                     /translation="MTPQPDPLLVCVGDLMVDVAVAAPALARGGDVEGAVRLGPGGSA
                     ANVAVWGAGAGGRARLVAGRGDDLAGRLLAAALAERGVELRPGDPAAGASGAMLVVME
                     AGERTFVADPGANLGLAKADVVAGLEGADAVFVSGYPLLRAATRPAAMAAAEVAGRAG
                     LPAVVDAASWPLLGGGAGEPVLGAAALAGTLLANRDEAAALAGRPDPAAAGAWLAARV
                     G"
     gene            complement(654..>2498)
                     /locus_tag="VFO47_02040"
     CDS             complement(654..>2498)
                     /locus_tag="VFO47_02040"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HET8787515.1"
                     /translation="LQLVHGRHDPGLRSGSTLVALDRLTAAGFVGRADAAQLAQAYRF
                     LRLVEHRLQLASERRTHTIPAGEEARRWLARTLGYRDDPEASALERFEADRRRHAAAV
                     RGLHEKLFYRPLLEAFGAVPAGLDPEGARERLAALGFANPDRAMASLRALTSGLSRRA
                     TLMRAMLPVMLPWLAEAPDPDGGLAALRGLAEALGDRAAFYGMVRDNPAAAELLCTVL
                     GTSRLLGDLLARHPELLTAMADQHRVAAVRGPAELVTSATAIVTRHADPAAAWDGLRR
                     FKRRELVRVAVRDLTGDLPVERVGTELSALAQACLGAGLLIAMREAGEGPPPVRLAVL
                     AMGKLGGTELSYTSDLDVLFCHEPMPGADPGVAGQAADRVVRELLRGLSAVTPEGTCF
                     EVDANLRPEGRNGPLSRTLGSYLAYWDRWAQPWERQALIKVRPVAGDAELAGRFCAEA
                     AARVYPDPLDPGVVAEVRRVKARVESERLPAGADPKLHLKLGPGGLADVEWTAQLLQL
                     RFGGAEPSVRVQPTLAALDALAGVGALEAGEAAWLADAYRLCLRIRNLAYLVAGRPVD
                     TLPTDPVALERLARSMGEPGRQRLLEDYRRATRRARRVVGTRFWDDPP"
BASE COUNT          283 a         1019 c          960 g          236 t
ORIGIN      
        1 cccgaccctc gcggccagcc aggccccggc cgccgccggg tcgggccgcc cagccagggc
       61 ggccgcctcg tcgcggttgg ccagcagggt gccggccagg gcggccgcgc ccagcacggg
      121 ctcgccggcc ccgccgccga gcagcggcca ggaggcggcg tcgaccaccg ccggcagccc
      181 ggcccggccg gcgacctcgg ccgcggccat ggccgccggc cgggtggcgg cccgcagcag
      241 cgggtagccg ctcacgaaca cggcgtccgc cccctccagg cccgcgacca cgtctgcctt
      301 ggccagcccg aggttggccc cggggtcggc cacgaaggtc cgctcccccg cctccatcac
      361 caccagcatg gccccgctgg ccccggccgc cgggtccccc ggccgcagct ccaccccccg
      421 ctcggccagg gccgcggcca gcagccgccc ggccaggtcg tccccccgcc cggccaccag
      481 ccgcgcccgc cctccagccc ccgcccccca caccgccacg ttggccgccg acccccccgg
      541 cccgagccgt accgccccct ccacgtcccc cccacgggcc agcgccggcg ccgccacggc
      601 cacgtccacc atcaggtccc cgacgcacac cagcagcggg tcgggctgcg gggtcacggg
      661 gggtcgtccc agaagcgggt gccgacgacg cggcgggcgc ggcgggtggc gcggcggtag
      721 tcctcgagga ggcgctggcg gccgggttcg cccatggatc tggccaggcg ttccagggcg
      781 acagggtcgg tggggagggt gtcgacgggg cggcccgcga ccaggtaggc gaggttgcgg
      841 atgcgcaggc agagccggta ggcgtcggcc agccaggccg cctcgccggc ctcgagggcg
      901 ccaaccccgg cgagggcgtc gagggcggcc agggtcggct ggacccggac ggacggctcg
      961 gcgccgccga accggagctg gagcagctgg gccgtccact cgacgtcggc caggccgccc
     1021 gggcccagct tgaggtggag cttggggtcg gccccggccg ggaggcgctc ggactcgacc
     1081 cgggccttga cgcgccgcac ctcggccacc acccccgggt ccagcgggtc ggggtagacc
     1141 ctggccgcgg cctcggcgca gaaccgcccg gccagctcgg cgtccccggc caccggccgc
     1201 accttgatca gggcctggcg ctcccacggc tgggcccagc ggtcccagta ggccaggtag
     1261 ctgccgaggg tccgcgacag gggcccgttg cggccctcgg ggcggaggtt ggcgtcgacc
     1321 tcgaagcagg tcccctccgg ggtgacggcc gacaggcccc gcagcagctc ccggaccacc
     1381 cggtcggcgg cctggccggc gaccccgggg tcggcgcccg gcatcggctc gtggcagaac
     1441 agcacgtcca ggtcggaggt gtagctcagc tcggtcccgc cgagcttgcc catggccagc
     1501 acggccagcc tgaccggtgg cggcccctcg ccggcctcgc gcatggcgat cagcagcccc
     1561 gcccccaggc aggcctgggc gagcgcggac agctcggtcc cgacccgctc caccgggagg
     1621 tcgccggtga ggtcgcgcac ggccacccgg accagctcgc ggcgcttgaa ccggcgcagc
     1681 ccgtcccagg ccgcggccgg gtcggcgtgc cgggtcacga tcgcggtcgc cgaggtgacc
     1741 agctcggccg gcccccggac cgcggccacc cggtgctggt cggccatggc cgtgagcagc
     1801 tcggggtggc gggccagcag gtcgccgagc agccgcgagg tgccgagcac ggtgcagagc
     1861 agctcggccg cggccgggtt gtcgcggacc atcccgtaga acgcggcccg gtcgccgagg
     1921 gcctcggcca gcccccgcag cgcggccagc ccgccgtccg ggtcgggggc ctcggccagc
     1981 cagggcagca tcaccggcag catcgcccgc atgagcgtgg cccgtcgcga cagccccgag
     2041 gtgagggcgc gcagcgaggc catggcccgg tcggggttgg cgaacccgag ggcggccagc
     2101 cgctccctcg ccccctccgg gtcgagcccg gctgggaccg ccccgaacgc ctccagcagc
     2161 ggccggtaga acagcttctc gtgcaggccc cggacggccg cggcgtggcg gcgccggtcg
     2221 gcctcgaacc gctccagggc gctggcctcg gggtcgtccc ggtaccccag cgtccgggcc
     2281 agccagcggc gcgcctcctc cccggccggg atggtgtggg tgcggcgctc gctggccagc
     2341 tggaggcggt gctcgaccag gcgcaggaac cggtaggcct gggccagctg ggcggcgtcg
     2401 gcccggccga cgaacccggc cgcggtcagc cggtcgagcg cgaccagggt cgagccggac
     2461 cgcagccccg ggtcgtggcg gccgtggacc agctggag
//
LOCUS       DASSWR010000125         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1134532, whole genome shotgun sequence.
ACCESSION   DASSWR010000125 DASSWR010000000
VERSION     DASSWR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134532"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFO47_02045"
     CDS             complement(<1..500)
                     /locus_tag="VFO47_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787516.1"
                     /translation="MEERLPGERLAELVELGDPAALLRAVDGLCASRDWAGLVDLRHR
                     LDEAVERGRPLWPVTTYVEYRMALDGPAPEAASVLRPGAARFALGPLTEVAATTHTFA
                     ELAPHLAVPAVAGAVAQERVLRGEDLREAPGAHAEVLELPLVLAPWEPEYALATYRPD
                     EREVPDP"
     gene            591..1301
                     /locus_tag="VFO47_02050"
     CDS             591..1301
                     /locus_tag="VFO47_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HET8787517.1"
                     /translation="MRHHDKATAGPGAPPSGDPALGAPASFRPTPYEVAQRRGHEVLR
                     RALLDGASGLLVAEGPGALTMRRVAGVVGCSTSVLYTMFGAKEGLADALYREGFERLR
                     RRLEAVPDRGDPLARLRELARAYRDNALAERAYYGVMFQRAIPRFEPSPASLAVAGAS
                     LEVLALAVRAAMDAGALRPGDPRAVAEVLWAAVHGAVSLELAGHFPDPAVAADRFQTL
                     ASAAILPFLADPPTSRRR"
     gene            1303..1706
                     /locus_tag="VFO47_02055"
                     /pseudo
     CDS             1303..1706
                     /locus_tag="VFO47_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1713..2516)
                     /locus_tag="VFO47_02060"
     CDS             complement(1713..2516)
                     /locus_tag="VFO47_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET8787518.1"
                     /translation="MSWPLYQVDAFTDEPFAGNPAAVCLLAADADPAWMQRVAAEMNL
                     PETAFLRPSAEAGRYGLRWFTPTVEVELCGHATLASAHVLFSEGLAEAGRPVRFDSAS
                     GPLTARPGAGGAIWLDFPATPAAPADPPGGLLEALGAGPVRWVGLGRLDYLVELEDEA
                     AVRDLAPDVGRLGWLGSRGVIVTAAASGTGAYDFVSRFFAPGAGIDEDPVTGSAHCAL
                     GPFWAGRLGRDELTGFQASARGGLVQVCPEGDRVLLGGRAVTVLRGQLV"
     gene            complement(2513..>3280)
                     /locus_tag="VFO47_02065"
     CDS             complement(2513..>3280)
                     /locus_tag="VFO47_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8787519.1"
                     /translation="RNHDELTLEMVTDEERDYMYAEYAKDPRMRRNIGISRRLAPLLD
                     NGRRQIELLYSLLLSLPGSPVMYYGDEIGMGDNIYLGDRDGVRTPMQWTGDRNAGFSK
                     ADPAALYLPFLMDPVYGYQACSVEAQERVSTSLLQWVRRMLQVRKQHPVFGIGDYEPL
                     YPDNRRVLAFYRQLGADVVLVIANLSRFAQPAELDLSRFHGLIPEEMFGRTPFPMIDD
                     EPYRLGLGPHGFYWFHLVEPPAEPDDTAMFRAIRTRP"
BASE COUNT          402 a         1226 c         1223 g          429 t
ORIGIN      
        1 gggtcgggga cctcgcgctc atcgggccgg taggtggcca gggcgtactc cggctcccac
       61 ggggccagca ccaggggcag ctccagcacc tcggcgtggg ccccgggcgc ctcgcgcagg
      121 tcctccccgc gcaggacccg ctcctgggcg acggcgccgg ccaccgccgg cacggccagg
      181 tgcggggcca gctcggcgaa ggtgtgggtg gtggccgcga cctcggtgag ggggccgagg
      241 gcgaagcggg ccgcccccgg ccggagcacg ctggccgcct ccggggcggg gccgtccagg
      301 gccatccggt actcgacgta ggtggttacc ggccacagcg gccgtccccg ctccaccgcc
      361 tcgtccagcc ggtgccgcag gtcgaccagg ccggcccagt cgcggctggc gcagagcccg
      421 tcgacggccc gcagcagggc cgccgggtcc ccgagctcga ccagctcggc cagccgctcc
      481 ccgggcaggc gttcctccac gcggcctcct ccatcacgtc gcgacgcgcc atcttgccat
      541 cctccgccaa caacattgtc atcgcataat aacaacgtta cagtggcggc gtgaggcacc
      601 acgacaaggc gacggcgggc ccgggcgccc cgccttccgg ggacccggcg ctgggcgccc
      661 cggcttcgtt tcgcccgacc ccctacgagg tcgcccagcg gcgtgggcac gaggtgctgc
      721 ggcgggcgct gcttgacggg gccagcgggt tgctggtggc cgaggggccg ggggcgctga
      781 ccatgcggcg ggtggccggg gtggtggggt gctcgacgag cgtgctctac acgatgttcg
      841 gggccaagga ggggctggcc gacgcgctct accgggaggg gttcgagcgg ctccggcgcc
      901 gcctggaggc ggtgccggac cggggcgacc cgctggcccg gctgcgcgag ctggcccggg
      961 cctaccggga caacgcgctg gccgagcggg cctactacgg cgtcatgttc cagcgggcca
     1021 tcccccggtt cgagccgtcg ccggcgtcgc tggccgtggc cggggccagc ctggaggtgc
     1081 tcgccctggc cgtgcgggcg gccatggacg ccggggcgct gcggcccggc gacccccggg
     1141 ccgtggccga ggtgctgtgg gcggcggtcc acggcgcggt cagcctggag ctggccggcc
     1201 acttccccga ccccgcggtc gccgccgacc ggttccagac cctggcctcg gcggcgatcc
     1261 tcccgttcct ggccgacccg ccaacgtcaa ggaggcgctg acatgtccga cgcgtccagg
     1321 cagctcgccg gcatcctgtt cgtggtcatc ccgacgatcg agttcggcgg ctggtcgctg
     1381 ctgcgcatga tcgcccgccg catccccggc tacctcgaca acccggtgcg ccagaacctg
     1441 ttccgggccg gccacgccca cgccggcgtg ctggtgctcc tggccctggt cggcatcctc
     1501 tacgtcgacc agaccgacct gtcgtcaggc gtccggacgc tcgtgcgcac cggcttcgcc
     1561 ctggccccga tcctgatctc ggccgggttc ttcctctcgg tgatcccgcc ccgggccgag
     1621 cgcccagccg catgatcacc ctggtctacc tgggtgccct cctgctggcg gccgccaccg
     1681 tcaccctggg cgtgagcctg ctctgaccgg gctcagacga gctggccgcg caggacggtg
     1741 acggcccggc cgccgagcag gacccggtcg ccctccgggc agacctggac caggccaccc
     1801 cgggccgacg cctggaagcc ggtcagctcg tcccggccca gccgcccggc ccagaacggg
     1861 cccagcgcgc agtgggccga cccggtcacc gggtcctcgt cgatcccggc cccgggggcg
     1921 aagaagcggg acacgaagtc gtaggccccg gtcccggagg cggcggcggt cacgatcact
     1981 ccgcggctgc cgagccaccc cagccgcccg acgtcggggg ccaggtcgcg cacggccgcc
     2041 tcgtcctcca gctcgaccag gtagtcgagg cggcccaggc cgacccagcg caccggcccg
     2101 gcccccagcg cctccagcag cccgccgggc gggtcggcgg gcgcggccgg ggtggccggg
     2161 aagtcgagcc agatggcccc gccggcgccg ggccgggcgg tcagcggccc gctggcgctg
     2221 tcgaaccgga ccggccggcc ggcttcggcc aggccctcgc tgaacagcac gtgggccgag
     2281 gccagggtgg cgtggccgca cagctcgacc tcgaccgttg gcgtgaacca gcgcagcccg
     2341 tagcggccgg cctcggccga cggtcgcagg aaggccgtct ccggcaggtt catctcggcc
     2401 gccacccgct gcatccaggc cgggtcggcg tcggccgcga gcaggcagac ggcggccggg
     2461 ttgcccgcga acggttcgtc ggtgaaggcg tcgacctggt agagcggcca gctcatggcc
     2521 gggtgcggat ggcccggaac atggccgtgt cgtcgggctc ggccggcggc tcgaccaggt
     2581 ggaaccagta gaagccgtgc ggccccaggc ccaggcggta gggctcgtcg tcgatcatcg
     2641 ggaacggcgt ccgcccgaac atctcctcgg ggatgagccc gtggaagcgg gacaggtcga
     2701 gctccgccgg ctgggcgaag cgcgacaggt tggcgatcac caggaccacg tcggccccga
     2761 gctggcggta gaaggccagc acccgccggt tgtcggggta cagcggctcg tagtcgccga
     2821 tgccgaacac cgggtgctgc ttgcgcacct ggagcatgcg ccggacccac tggagcagcg
     2881 aggtcgacac ccgctcctgg gcctcgacgc tgcacgcctg gtagccgtag accgggtcca
     2941 tcaggaacgg caggtacagg gcggccgggt cggccttgga gaagccggcg ttgcggtccc
     3001 cggtccactg catcggggtc cggaccccgt cgcggtcgcc caggtagatg ttgtcgccca
     3061 tgccgatctc gtcgccgtag tacatcaccg ggcttcccgg cagcgacagc agcaggctgt
     3121 agagcagctc gatctgtcgc cggccgttgt ccagcagcgg ggccaggcgc cgcgagatgc
     3181 cgatgttgcg gcgcatccgc gggtccttgg cgtactcggc gtacatgtag tcgcgttcct
     3241 cgtcggtcac catctcgagg gtcagctcgt cgtggttgcg
//
LOCUS       DASSWR010000126         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1022242, whole genome shotgun sequence.
ACCESSION   DASSWR010000126 DASSWR010000000
VERSION     DASSWR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022242"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1152)
                     /gene="rpsB"
                     /locus_tag="VFO47_02070"
                     /pseudo
     CDS             complement(268..1152)
                     /gene="rpsB"
                     /locus_tag="VFO47_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            complement(1396..1821)
                     /locus_tag="VFO47_02075"
     CDS             complement(1396..1821)
                     /locus_tag="VFO47_02075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET8787520.1"
                     /translation="MPLAGSVVRGFDARAGPYGPGHRGIDVAASAGEVVRAPAAGEVV
                     FAGPVAGTTWVSIRVAPGVVVTVGPLLDEVAPGGRVRARAPLGRVGPGHEPGPVGGAQ
                     TLHLSVRVDGEYLDPLAYLVDRPRPRLAPLPAPGALPSP"
BASE COUNT          275 a          961 c          924 g          348 t
ORIGIN      
        1 ggcgcccccg gagtcggcct gggtctcgtc ggcggtcccg gccccggcgg cctcgatggc
       61 ctcgtcactg gcctccggct cggtggtccc gccctcgggc gtggcctcct cggggacctc
      121 ggtctcggcc ttggtgggct cggcggcggc ctcgggctgg gtcgcgccct tggccttgcc
      181 ccgtgacggg gtcgtggctc ggccgccgga gctctgggtc ctgctccggc ccgcggcgcc
      241 ctgggccccg ccgcggccgg tggccgccgg cttgtcggcg ggcgaggccg gctggtcggc
      301 ggcggagtcg gccgccgggg ccgcggcgct ctcggcgcgc ccgaagcggc tggcctcggc
      361 cgcatcgccg ccgtcggact cgagcagctc gcgctcccac tcggccagcg gctcctcgtc
      421 ggcggggctg gccgagatcg gggccgcgcc ctccttgccg gcggcctcgg ccgcgtactg
      481 gctgcgcagc cccagcccct cggcgaccgc gtcggccacg atccgggtga gcaggctccc
      541 ggcccggatg gcgtcgtcgt tgccggggat cgggtagtcg acctcgtccg ggtcgcagtt
      601 ggtgtcgagg atggccacca ccgggatgcc gagcttgcgt gcctcggtca cggcgatgtg
      661 ctccttgcgg gtgtcgacca cccagatcgc ggccggcagc cgggtcatgt cgcggatgcc
      721 gcccaggttc cgctccagct tctcgcgctc gtgcgcgagc cgcaggacct ccttcttggg
      781 cagcacgtcg gtcgcgccgg tggccaccat cgcctccagc tccttgagcc gctggagccg
      841 cttgtagatg gtggtgaagt tggtcagcat cccgcccagc cagcggaagt tcacgtacgg
      901 catcccgacc cgctcggcct gggtcttgat cgactcctgg gcctgcttct tggtgccgac
      961 gaacagcacg ttgccgcccc tggccaccgt gtcgcgcagg aacccgtagg tcgactcgat
     1021 ggcggcgctg gtcttctgca ggtcgatgat gtagatgccg ttgcgctcgc cgtagatgaa
     1081 gcgcttcatc ttcgggttcc agcgccgggt ctggtgcccg aagtggacgc cagcttcgag
     1141 cagctggcgc atggtgacga ctgccacgcc tgttcctccc tgccgttccg gttcggcctc
     1201 cgcccgctgt gctcccacag tcgtcggatc gtgggggttc cgggtcctcg acccgctcgg
     1261 gttccccgga gcgggcaccg tcgagtcccg tcgacgggcg tgcgttgtag cccggactcc
     1321 cgggcccgcg gcggacgata gcacaagccc cccagctccc gccccaccgg cccacccctg
     1381 ccagcccagt gctggtcagg ggctgggcag cgcaccaggc gccggcaatg gggcgagccg
     1441 gggcctgggc cggtcgacca ggtaggccag gggatccagg tactccccgt ccacccgcac
     1501 gctcaggtgc agggtctgcg ctccgccgac cgggccgggc tcgtgccctg ggccgacccg
     1561 cccgagcggg gctctcgctc ggacccggcc cccgggcgcc acctcgtcca gcagcgggcc
     1621 gacggtcacc acgactcccg gtgcgacccg gatgctgacc caggtggtcc ccgcgaccgg
     1681 gccggcgaac acgacctccc cggccgccgg cgcccgcacc acctccccag cggaggcggc
     1741 gacgtcgatc ccccggtgcc cgggaccgta gggcccggca cgcgcgtcga acccccgcac
     1801 caccgagccg gccaacggca tccacagcgg cggcacccga gggcgatccg gcgccgcggt
     1861 cggcggcccc ggcgtcggcg gcggcggggg gggacgctcg gccaccgcgg cgggcgcggc
     1921 gacgggcgcc aacgccccga ccgtcaagag gagggccagg aggaacggcg cccagcccgg
     1981 ccaaggcctt cccgccggct cggggcaggt cgtcgacatc cgggactcca ggctcctcag
     2041 gacgccaggg cgtgaccgcg acggcgtcgt tccgactggg ccgacgctac ccggcgggat
     2101 cggccagcgg gccgtggctc acgaaattgt ggatggcttc gtcggccggc ggcccggcct
     2161 gctcgcgctc ggcgcctgcg gccccggctg gagcacaacg tgaggaacga gaacgcgatc
     2221 accagggcga cgaccaggaa caccgacgtc ggcgcgtcga gcaggacggc gacggcgagg
     2281 gccgccacca ccccgacgac cacgagggcg atgctcggtc gcgtcgatca ggccgacgat
     2341 gccgtggaca ccaggtcggc ctgctcgatg tcggtggccg gccgaccgag acccggccgg
     2401 ccacgaccgt gtcggccgtg gcggcgtttg gtacccggct cgccgtgcct ggcggtcggc
     2461 gccccggctc gcctcgtcag ctcaggcgcg cggatgcgcc tgctcgta
//
LOCUS       DASSWR010000127         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1248056, whole genome shotgun sequence.
ACCESSION   DASSWR010000127 DASSWR010000000
VERSION     DASSWR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248056"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="VFO47_02080"
     CDS             complement(<1..2183)
                     /locus_tag="VFO47_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET8787521.1"
                     /translation="MGQDDSIGREVLPIPERPHVGLVTYDAKDPATSFPPIESLRPPA
                     GAPNVLVVLLDDVGFGASSAFGGPISTPTAERLAAQGLKYTRFHTTALCAPTRAALLT
                     GRNHHAVGMGVITELATSAPGYNSLRPNTCAPLAEVLKLNGYSTAQFGKCHEVPVWQA
                     SPMGPFDAWPTGSGFEHFYGFIGGETNQYAPAIYQDTVPVEPDRTPEEGYHFTEDMTD
                     RAIDWVRQQKALMADKPFFVYFAPGATHAPHHVPTEWADKYKGRFDQGWDRLREETIQ
                     RQKAIGVVPADTELTRRHDEIPAWDDMPDELKPVLARQMEVYAGFLEHTDHHVGRLID
                     ALADLEILDDTLVYYIIGDNGASAEGTLQGTFNEMISMNGAAALETTEFMAARIDKFG
                     TPEAFNHYAVGWAHAMDTPYQWTKQVASHWGGTRNGTIVHWPAKIQAKGETRSQFHHV
                     IDVAPTVLEVAGLPEPTMVNSVQQQPLQGTSMAYSFNEAEAADRHDVQYFEMFCNRGI
                     YHQGWTAVTRHSTPWAFGTKLPAFDEDVWELYGPDDWTQARDLAAEQPGKLAELQRLF
                     LLEAGKYNVFPLDDRRVERFNADLAGRPQLIKGNRQLLFGGMGRLSENSVVVTKNKSH
                     SITAQLVVPDAGAQGVIVAQGGAYGGWSLYAKDGRPVYCYNLFGLQRFKVYGEEPIPA
                     GEHQVRMEFAYDGGGPARGGTVRLYVDGALVGEGRVEATQPLVFSL"
BASE COUNT          430 a          958 c         1012 g          500 t
ORIGIN      
        1 agtgagaaca caagaggctg cgtggcctcg acccgcccct cgcccacgag cgctccgtcg
       61 acgtacagcc tgaccgtgcc tcccctggcc ggaccgcccc cgtcgtaggc gaactccatc
      121 cggacctggt gctcgccggc cgggatgggc tcctcgccgt agaccttgaa gcgttgcagc
      181 ccgaacaggt tgtagcagta gaccggccgc ccatctttgg cgtacaggct ccagccgcca
      241 taggcgccgc cctgggccac gatcacccct tgggcgccgg cgtcggggac gacgagctgg
      301 gcggtgatcg agtgggactt gttcttggtg accacgaccg agttctcgct cagccggccc
      361 atgccgccga acagcagctg gcggttcccc ttgatgagct gggggcggcc ggccaggtcg
      421 gcgttgaacc gctcgacccg ccggtcgtcc agtgggaaca cgttgtactt gccggcttcc
      481 agcaggaata gccgctgcag ctcggccagc ttgcctggct gttcggcggc caagtcgcgg
      541 gcctgggtcc agtcgtcggg gccgtacagc tcccagacat cctcgtcaaa ggccggcagc
      601 ttggtcccaa aggcccaagg ggtgctatgg cgggtcaccg ccgtccagcc ctggtggtag
      661 atgccgcggt tgcagaacat ctcgaagtac tggacgtcgt gccggtcggc cgcctccgcc
      721 tcgttgaacg agtaggccat gctggtcccc tggaggggct gctgctggac gctgttgacc
      781 atggtcggct cgggcagccc agccacttcc agcacggtcg gagcgacgtc gatgacgtgg
      841 tggaactggc tgcgcgtctc gcccttggcc tggatcttcg cgggccagtg gacgatggtg
      901 ccgttacggg tgccgcccca gtgggaggcg acctgcttgg tccactgata gggggtgtcc
      961 atggcatggg cccagccgac ggcgtagtgg ttgaaggctt ccggggtgcc gaacttgtcg
     1021 atccgcgccg ccatgaactc ggtcgtctcc agcgcggcgg caccattcat gctgatcatc
     1081 tcgttgaagg tgccctgcaa ggtcccttcg gccgaggcgc cgttgtcgcc gatgatgtag
     1141 tagaccagcg tgtcgtccag gatctccagg tcggccaggg cgtcgatgag ccggccgacg
     1201 tggtggtcgg tgtgctccag gaacccggcg tacacctcca tctggcgggc cagcaccggc
     1261 ttcagctcgt cgggcatgtc gtcccaggcc gggatctcgt cgtggcggcg ggtaagctcg
     1321 gtgtcggccg gaaccacgcc gatcgccttc tgccgctgga tcgtctcctc gcgcagccgg
     1381 tcccagccct ggtcgaagcg gcccttgtac ttgtcggccc actcggtagg gacatggtgg
     1441 ggggcgtggg tcgccccggg ggcgaagtag acgaagaacg gcttgtcggc catcagcgcc
     1501 ttctgctggc gtacccaatc gatcgcccgg tcggtcatgt cctcggtgaa gtggtagccc
     1561 tcctccgggg tccggtcggg ctccaccggc acggtgtcct ggtagatcgc cggggcgtac
     1621 tggttggtct ccccgccgat gaagccgtag aagtgctcga acccgcttcc ggtcggccag
     1681 gcgtcgaacg gacccatcgg gctggcctgc cacaccggca cctcatggca cttgccgaac
     1741 tgggcggtgg agtagccgtt gagcttgagg acctcggcca agggcgcgca ggtgttgggg
     1801 cgcagcgagt tgtacccggg cgcggaggtg gcgagctcgg tgatcacgcc catcccgacg
     1861 gcgtggtggt tgcggcccgt gagcaaggcg gcccgggtcg gggcgcacag cgcagtggtg
     1921 tggaaccggg tgtacttcag gccctgggcg gccagccgct cggcggtcgg ggtgctgatg
     1981 gggccaccga aggcgctgga ggcaccgaac ccgacgtcgt ccagcagcac caccaggacg
     2041 ttgggggcgc cagcgggcgg ccgcagcgac tcgatcggtg ggaagctggt ggcggggtcc
     2101 ttggcgtcgt aggtgaccag cccgacatgc ggccgttccg ggatcggtag cacctcgcgc
     2161 ccgatcgaat cgtcctgacc catctgactc gcccccaatt gccttgtccg gcaccacggt
     2221 ccgagccgac catagagccg agccaggtga gctgcatccc ccataacagg tgatttcgcc
     2281 atgcgccaag catcggacca cgacgtccta gaggtcgcgc cccggggccg gtcagcatcc
     2341 gcgatggctg ctccacagca ccggagcttg accgcgatgc tcggcctatc ccgtgacctc
     2401 agtgtccacc gtccccccgg tccaagggcg tggcagaccg cccgaccggg gcaacaggaa
     2461 ggggcgcacc atgaccgaga ccagcgtcag cttcgccggt gacctcaccg acgaccccag
     2521 ctccaacccg ccgagcgcgt catcgcccgg gccatgtttc gggtgggtgt gcggtcggcg
     2581 ggagccggaa gcgtcgttct gatgcgggac ctggccgaca gagcggtcgc ctgacgacaa
     2641 ggcggccgta cccggtttgg tgacatcacc actgactggc cgttttgacc atagaaaccc
     2701 gatggcatga atcgccgctg gcacggggcg gcgccggcgt cggcccacag agtcgttgtg
     2761 ctaatttttg gtttctatga cggtagcgga cgttccgttc gacctcgttg aggcgaagct
     2821 cggcgcgccg acgatccgac ccggcaccgt ggccaagcag gacgtgatcg aacggttgcg
     2881 ggcatcgcgg ctgccctttg
//
LOCUS       DASSWR010000128         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_598537,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000128 DASSWR010000000
VERSION     DASSWR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598537"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFO47_02085"
     CDS             complement(<1..376)
                     /locus_tag="VFO47_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787522.1"
                     /translation="MTSTEDPGTDPSQGSERLEPGPLFSRVVRLDDGLDPLEEAGDDG
                     FVWHSPGGALVGLGVAARVPVGTGPGRVERAAETVASLLEAAEVADPDRTGLGPAAVG
                     ALPFHPATPGELVVPALLLRTDP"
     gene            complement(414..1190)
                     /locus_tag="VFO47_02090"
     CDS             complement(414..1190)
                     /locus_tag="VFO47_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET8787523.1"
                     /translation="MIQSVGRAARILKALGSESARLGVTELAERLGLAKATVYGLLRT
                     LEAHQLVEQDSETGKYRLGPALLQLGNAFLDNNELRARSLLWAETLAGRAREAVHVGV
                     LYGPNVLLVHHVFRPDNSLQILQVGASIPWHACALGKATVAYLEPEAQGELLAGPLPR
                     LTGRTLTSGTVLRATLEAVVETGVAVEDQEAIVGEAGIAAALFDNRGAVVGAIGLAGP
                     VERLLPDGPAADAVAAVKEAARGLSRELGAGRPAVPGAGA"
     gene            complement(1187..1864)
                     /gene="dhaL"
                     /locus_tag="VFO47_02095"
     CDS             complement(1187..1864)
                     /gene="dhaL"
                     /locus_tag="VFO47_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET8787524.1"
                     /translation="MDAACVRLWLGEVAARLEAERDFLTQLDAAIGDADHGVNMHRGF
                     TAVMARIGAVEEATPGQLMKLAGRTFVSTVGGAAGPLYGTAFLQAGEALGDHERFGPR
                     LLGEALRASLEGVQRVGAASVGDKTMVDALTPALGAFELELRMGGSLETAASGAREAA
                     AEGMRATIPLQARKGRASYLGPRSVGHQDPGATSTALMFAALEQAVLSDRSQRFAAGA
                     RRRVSGP"
     gene            complement(1936..>2238)
                     /locus_tag="VFO47_02100"
     CDS             complement(1936..>2238)
                     /locus_tag="VFO47_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HET8787525.1"
                     /translation="GIGTDATLIAEAIAAADEGDGVLVLVDLGSAVLSAQLAIDELVE
                     EGTRGRVRIAEAPVVEGAVVAAIQASTGSSLDDVDQAARGAATMAKVKAPEHQAGE"
BASE COUNT          263 a          854 c          860 g          261 t
ORIGIN      
        1 cggggtcggt gcgcagcagc agggccggga cgaccagctc gcccggggtg gccgggtgga
       61 acgggagggc gccgaccgcc gccgggccca ggccggtgcg gtcggggtcg gcgacctcgg
      121 ccgcctcgag caggctggcg accgtctcgg cggcccgctc gacccggccc ggtcccgtgc
      181 ccacggggac gcgggcggcc acgcccaggc cgaccagggc gccgcccggg gaatgccaca
      241 cgaagccgtc gtcgccggcc tcctcgagcg ggtcgagccc gtcgtcgagg cggacgaccc
      301 ggctgaacag gggaccgggc tccagccgtt cggacccttg ggacgggtcg gtcccggggt
      361 cctccgttga cgtcatggcg ccatcgtagg ggcgggatgc gccgtcgcgc gactcaggcg
      421 ccggcgccgg ggacggccgg gcggccggcg cccaattcgc gcgacagccc gcgggccgcc
      481 tccttgaccg cggccaccgc gtcggcggcc gggccgtcgg ggagcaggcg ctcgaccggg
      541 ccggccagcc cgatggcgcc gaccaccgcc ccccggttgt cgaacagcgc ggcggcgatg
      601 ccggcctccc cgacgatcgc ctcctggtcc tcgacggcca ccccggtctc gaccaccgcc
      661 tccagggtcg cccggaggac ggtccccgac gtcagggtcc ggcccgtgag ccggggcagc
      721 gggccggcca ggagctcgcc ctgggcctcc ggctccaggt aggcgacggt cgccttgccg
      781 agggcgcacg cgtgccaggg gatcgacgcc cccacctgga ggatctggag cgagttgtcg
      841 ggccggaaca cgtggtggac gagcagcacg ttggggccgt agaggacccc gacgtggacg
      901 gcctcgcggg cccggccggc cagggtctcg gcccacagca gggagcgggc ccgcagctcg
      961 ttgttgtcca ggaaggcgtt gccgagctgg agcagggccg gtcccagccg gtacttgccg
     1021 gtctcggagt cctgctcgac cagctggtgg gcctcgagcg tgcgcagcag gccgtacacg
     1081 gtcgccttgg ccaggccgag ccgctcggcc agctcggtca cgcccagccg ggccgactcc
     1141 gaccccaggg ccttgaggat ccgggccgcc cgcccgaccg actggatcat ggcccgctca
     1201 cccggcgcct ggccccggcg gcgaaccgct gggagcggtc ggagagcacg gcctgctcga
     1261 gggcggcgaa catcagcgcc gtcgaggtcg cgcccgggtc ctgatggccg acgctgcgcg
     1321 ggcccagata ggaggcgcgg cccttgcggg cctggagcgg gatggtggcc cgcatcccct
     1381 cggccgccgc ctcccgggcg ccgctggccg ccgtctccag cgacccgccc atgcgcagct
     1441 ccagctcgaa ggcgccaagg gccggggtga gggcgtcgac catggtcttg tccccgaccg
     1501 aggcggcccc gacccgctgg accccctcga gcgaggctcg cagcgcctcc cccagcagcc
     1561 gcggcccgaa gcgctcgtgg tcgccgaggg cctcgccggc ctggaggaac gcggtgccgt
     1621 agagcggccc ggcggcgccg ccgacggtgg agacgaaggt ccggccggcg agcttcatca
     1681 gctggccggg ggtggcctcc tcgacggcgc cgatcctggc catgaccgcg gtgaagccgc
     1741 ggtgcatgtt gacgccgtgg tcggcgtcgc cgatggcggc gtcgagctgg gtcaggaagt
     1801 cccgctcggc ctccaggcgg gcggcgacct ccccgagcca gagccgaacg caggccgcgt
     1861 ccacgatcct ggctgcctcc cgtcggggtc cgggcaccaa acggccccat ggtactgcga
     1921 gggcggccgt catcctcatt ccccggcctg atgctccggc gccttgacct tggccatggt
     1981 ggccgcgccc ctggccgcct ggtcgacgtc gtcgaggctg gagccggtgc tggcctggat
     2041 ggcggcgacc acggcgccct cgacgaccgg ggcctcggcg atgcggaccc gcccgcgggt
     2101 cccctcctcg accagctcgt cgatggccag ctgggccgag agcacggccg atcccaggtc
     2161 gaccaggacc agcaccccgt cgccctcgtc ggcggccgcg atggcctccg cgatcagggt
     2221 ggcgtcggtg ccgatgcc
//
LOCUS       DASSWR010000129         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_994237,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000129 DASSWR010000000
VERSION     DASSWR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994237"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFO47_02105"
     CDS             <1..421
                     /locus_tag="VFO47_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET8787526.1"
                     /translation="SPWMKAIHDTLAQLFRGSHDADRRESYTQDLVQGLLDDGTVQLE
                     PLTFIRGRTFARTFAILDEAQNVEYGVLRSLASRLGEGSKLVLCHDTSQVDHPYVDPD
                     SGVAAMIERLKGEPLFGHVTLVKGERSPVAELVARKL"
     gene            433..750
                     /locus_tag="VFO47_02110"
     CDS             433..750
                     /locus_tag="VFO47_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787527.1"
                     /translation="MSQPQPADELSPTDRELLQRAELALTQLNNRHGLEADQSATLTA
                     IRLRLEGKSRASLDDLLVAGKDLGGKDPLTEAMTGRRQGPSFDDLVAGAEQKPKKSLD
                     DLL"
     gene            843..>2410
                     /gene="ggt"
                     /locus_tag="VFO47_02115"
     CDS             843..>2410
                     /gene="ggt"
                     /locus_tag="VFO47_02115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8787528.1"
                     /translation="MPRPVRALLTVLLLILCLVAATVPAQAGRKPVKQPVATGTGGAI
                     ATVDLDASAAGLEVLRHGGNAVDAAVAAAATLGVTEAYSAGVGGGGFMVVHLAKERRV
                     VTIDGRETAPAAFREDSFIDPATGQPIPFAERVTSGLGVGVPGTVATWQRALRRYGTR
                     SLGKLLQPAIRVAERGFVVDQTFHDQTASNAARFAAFPATAELFLPGGAPPPVGSVLR
                     NPDLARTYRLLARGGTHPFYRGRIAEAIAETVQDPPVDPASTLNVRPGLMTEADLAAY
                     RALDRPPTRVGYRGYQVYGMGPPSSGGSTVGEALNILEGYDLGGESYTQALFHYLEAT
                     RLAFADRNRWVGDPDFVDVPLGGLLSQGFADERRCLIGDRANVSPVPPGDPFPPFGPG
                     CPAGVAVAGPAERPATTHLTVADRWGNVVSYTLTIEQTGGSGIVVPGYGFLLNNELTD
                     FNAVPLFPGVPDPNLPAPGKRPRSSMSPTIVFRDGRPVLAVGSPGGATIITTVLQILV
                     EHLDRGRSLPEAIAA"
BASE COUNT          329 a          976 c          805 g          300 t
ORIGIN      
        1 ctccccctgg atgaaggcca tccacgacac cctggcccag ctgttccggg gcagccacga
       61 cgccgaccgg cgcgagagct acacccagga cctggtccag gggctgctcg acgacggcac
      121 ggtgcagctg gagccgctga cgttcatccg cgggcgcacc ttcgcccgca ccttcgcgat
      181 cctcgacgag gcccagaacg tcgagtacgg ggtgctgcgc tcgctggcca gccggctcgg
      241 cgagggctcc aagctggtcc tgtgccacga cacctcccag gtcgaccacc cctacgtcga
      301 cccggactcc ggggtggcgg cgatgatcga acggctcaag ggcgagccgc tgttcggcca
      361 cgtcaccctc gtcaagggcg agcgcagccc ggtcgccgag ctggtcgccc gcaagctgta
      421 ggaggccgcc ccatgtccca gccccagccc gccgacgagc tcagcccgac cgaccgggag
      481 ctgctgcaac gggccgagct ggccctgacc cagctcaaca accgccacgg cctcgaggcc
      541 gaccagtcgg ccaccctgac cgcgatccgg ctgcgcctgg agggcaagtc ccgggcgtcc
      601 cttgacgacc tgctggtcgc cggcaaggac ctcggcggca aggacccgct gaccgaggcc
      661 atgaccggcc gccgccaggg ccccagcttc gacgacctgg tcgccggggc cgagcagaag
      721 cccaagaagt ccctggacga cctgctctga cctgagcaac ccaggctcgg acaaaccaag
      781 ccaaaggttg catggtggac tagactccgt aggctgccgt cgcacctgcc ggaggtcccg
      841 cgatgccgcg acccgtccgt gccctgctga ccgtcctgct cctcatcctc tgcctggtcg
      901 ccgcgaccgt ccccgcccag gctgggcgga aacccgtcaa gcagccggtc gccaccggca
      961 ccggcggggc gatcgccacc gtcgacctgg acgcctccgc ggccgggctg gaggtgctgc
     1021 ggcacggcgg caacgccgtc gacgccgcgg tcgcggccgc ggccaccctg ggcgtcaccg
     1081 aggcctactc ggccggcgtc ggcggcggcg gcttcatggt cgtccacctc gccaaggagc
     1141 gccgggtggt caccatcgac ggccgcgaga ccgcccctgc ggcgttccgt gaggacagct
     1201 tcatcgaccc ggccaccggc cagcccatcc ccttcgccga gcgggtcacc agcggcctcg
     1261 gggtcggggt gccgggcacg gtggcgacct ggcagcgggc cctgcgcagg tacgggaccc
     1321 ggtccctcgg caagctgctc cagccggcca tccgggtggc cgagcgcggc ttcgtggtcg
     1381 accagacctt ccacgaccag acggccagca acgccgcccg cttcgccgcc ttcccggcca
     1441 ccgccgagct gttcctgccc ggcggggcgc cgccgccggt cgggtcggtg ctgcgcaacc
     1501 ccgacctggc ccggacctac cggctgctgg cccgcggcgg cacccacccc ttctaccggg
     1561 gccgaatcgc cgaggcgatc gccgagaccg tccaggaccc gcccgtcgac ccggcctcga
     1621 ccctgaacgt ccggcccggc ctgatgaccg aggccgacct ggccgcctac cgggccctcg
     1681 accggccccc gacccgggtc ggctaccgcg gctaccaggt ctacggcatg ggcccgccgt
     1741 ccagcggcgg ctccaccgtc ggcgaggccc tcaacatcct ggagggctac gacctgggcg
     1801 gcgagtccta cacccaggcg ctgttccact acctggaggc gacccggctg gccttcgccg
     1861 accgcaaccg ctgggtcggc gacccggact tcgtcgacgt gcccttgggt ggcctgctca
     1921 gccaggggtt cgccgacgag cgccgctgcc tcatcggcga ccgggccaac gtcagcccgg
     1981 tgccgcccgg cgacccgttc ccgccgttcg ggcccggctg cccggccggg gtcgccgtgg
     2041 ccggcccggc cgagcggccg gcgaccaccc acctgacggt ggccgaccgc tggggcaacg
     2101 tcgtcagcta caccctcacc atcgagcaga ccggcggcag cggcatcgtc gtgcccggct
     2161 acgggttcct gctcaacaac gagctgaccg acttcaacgc cgtgccgctg ttcccgggcg
     2221 tgcccgaccc caacctgccc gcccccggca agcggccccg ctcctcgatg agcccgacga
     2281 tcgtgttccg cgacggccgg ccggtgctgg ccgtgggctc gccgggcggg gccaccatca
     2341 tcaccaccgt gctccagatc ctggtcgagc acctggaccg gggccggagc ctgcccgagg
     2401 cgatcgccgc
//
LOCUS       DASSWR010000130         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_87929,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000130 DASSWR010000000
VERSION     DASSWR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87929"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..2896)
                     /locus_tag="VFO47_02120"
     CDS             complement(314..2896)
                     /locus_tag="VFO47_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET8787529.1"
                     /translation="MPVKAVIMAGGEGTRLRPLTANQPKPMLPVGNRPIMEHIVRHIR
                     DYGFKDIVVTVQFLASQVRNYFGDGADMEVDLTYATEPTPLGTAGSVRNAAEMLGETF
                     VVISGDALTDIDLDEVVSFHRRRGALATVVLKRMPDPLEFGIVITTDDGRIERFLEKP
                     HWGQVFSDTINTGIYVLEPEVFDHIPEGEPSDFAGDVFPKLLDQGAPLFGFVAEGYWE
                     DVGSLEAYQRAHQDILDGKVKVDVRGFQVRPGVWLGEGAELDPDAVLTAPVLIGDFTK
                     VEAGARLREYSVVGSGVIVKRDAFLHRAIVHDNAYIGPGTSLRGCVVGRSADVKEGAR
                     VEDGVVVGDNAHIGHGAILQPNVKVYPYKTVEPGAIVTKSIIWEGRGARGLFDEHGVT
                     GIGNVDLTPEMVLRVATAFGSTLKKGSRVTLGRDASRVSRALKRAIIAGLNSAGVTCE
                     DLELAPAPSVRFSAARMGASGGMYVRTSPRDSQSVELSVFGGDGSDIDEGTRRRIERT
                     YYREEFRRAFGQEMGELRYPPRAVEHYAVALVHSLDDAVVRHRGFKAVIDAGGGTASL
                     VLPHLLSRLSLDTLIVNGHVDENRVAPTEDERQADLDRLAKLVTASGADLGVLFDAVG
                     ERIVLVDERGAILGLDRALLLFVDLIVRTEEGGGIALPVSTTRLAADLATRHGRQVHG
                     SKLATSAIMRTAQLDGVVFAGAEGGGYVFPALHPAYDGLLSFGKLLELLAVHDTTLGA
                     AVDALPEPSVVRRRVPTAWERKGAVMRQLVEASKGRRVDDTDGVKVFHPPDGSAEPAG
                     GPGESAGGPGPGAGAWGDDWVLVIPDTVEPITHLWAEAGDEAGAVALADEYEALIRRA
                     AELD"
     gene            complement(3085..>3360)
                     /locus_tag="VFO47_02125"
     CDS             complement(3085..>3360)
                     /locus_tag="VFO47_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET8787530.1"
                     /translation="RDLVLLCGWPILKRRGIEPPEVIFLGKAATLELLLALPILTLGA
                     TGLAVADLARLLGLALLWAGVVMYYLAGAVYVRMVLERLGHRAEAGS"
BASE COUNT          461 a         1198 c         1244 g          457 t
ORIGIN      
        1 gctcctgggt ggccagcagc ccgccggcca gcacgaccac cagcagcccg gccaggagcg
       61 cccgcggcat ccgggcgagc cggcgcaggg ctgacgccgc cgactccggt atccggcgaa
      121 gtcggtcaga cggctcgttg ggctcggcgg gcgggtcggc gaccgagatg gtgggctcct
      181 tcttgcatgc cgtccggtcg ggccggacgg cgcgacaccg gccgagagcg ggggaagcgg
      241 ccgggcgaac ggagagccta cggctccggg cgcctccggg caagcccatc ccggcgcagt
      301 gtggacaagc tggtcagtcc agctcggcgg cccggcggat gagcgcctcg tactcgtcgg
      361 ccagggcgac ggcgccggcc tcgtcaccgg cctcggccca caggtgggtg atcggctcca
      421 cggtgtcggg gatgaccagg acccagtcgt ccccccaggc acccgccccg ggacctggcc
      481 caccggccga ctcccccggc ccaccggcgg gctccgccga cccgtccggc gggtggaaga
      541 ccttgactcc gtcggtgtcg tcgacccgcc ggcccttgga cgcctccacc agctggcgca
      601 tgaccgcgcc cttgcgctcc caggcggtcg ggacccggcg gcggaccacc gacggctcgg
      661 gcagggcgtc cacggcggcg cccagggtgg tgtcgtggac ggccagcagc tccagcagct
      721 tgccgaacga cagcaggccg tcataggcgg ggtgcagcgc cgggaagacg tagcccccgc
      781 cctcggcacc ggcgaacacc accccgtcga gctgggcggt ccgcatgatg gcgctggtcg
      841 ccagcttgct cccgtggacc tggcggccgt ggcgggtggc caggtcggcg gccagccggg
      901 tggtggagac gggcagggcg atgccgccgc cctcctcggt ccgcacgatc aggtcgacga
      961 acagcagcag ggcccggtcc aggccgagga tggcgccgcg ctcgtcgacc aggacgatcc
     1021 gctccccgac ggcgtcgaac agcacgccca ggtcggcccc ggaggcggtg accagcttgg
     1081 ccaggcggtc gaggtcggcc tggcgctcgt cctcggtggg ggcgacccgg ttctcgtcca
     1141 cgtggccgtt cacgatcagg gtgtcaaggc tcaacctgga caagaggtgc gggaggacga
     1201 gcgaggccgt gcccccgccg gcgtcgatca ccgccttgaa gccgcggtgg cgcacgaccg
     1261 cgtcgtccag gctgtggacc agggcgacgg cgtagtgctc gaccgcccgc ggcgggtagc
     1321 gcagctcgcc catctcctgc ccgaacgccc ggcggaactc ctcccggtag taggtgcgct
     1381 cgatgcgccg cctggtcccc tcgtcgatgt cgctgccgtc gccgccgaac acggacagct
     1441 cgaccgactg gctgtcccgg ggcgaggtgc gcacgtacat gccgcccgac gcgcccatcc
     1501 gggcggccga gaaccgcacc gagggggccg gggccagctc caggtcctcg caggtcaccc
     1561 cggccgagtt cagcccggcg atgatggccc gcttgagggc gcgcgacacc cggctggcgt
     1621 cccgccccaa ggtgaccctc gaccccttct tgagggttga accgaaggcg gtggcgaccc
     1681 gcaggaccat ctcgggagtc aggtcgacgt tgccgatgcc ggtcaccccg tgctcgtcga
     1741 acagcccgcg ggcgccccgg ccctcccaga tgatggactt ggtgacgatc gcgcctggtt
     1801 cgaccgtctt gtagggatag accttgacgt tgggctgcag gatggccccg tggccgatgt
     1861 gggcgttgtc gccgacgacc acgccgtcct cgacccgggc gccctccttg acgtcggcgc
     1921 tgcgaccgac gacgcagccg cgcaggctcg tcccggggcc gatgtaggcg ttgtcgtgga
     1981 cgatcgcccg gtgcaggaag gcgtcgcgct tcacgatcac gcccgagccg accacgctgt
     2041 actcgcgcag gcgggcgccc gcctcgacct tggtgaagtc gccgatgagg acgggcgcgg
     2101 tcaggacggc gtcggggtcg agctcggccc cctcccccag ccagaccccg ggccggacct
     2161 ggaagccgcg cacgtcgacc ttgaccttgc cgtcgaggat gtcctggtgg gcgcgctggt
     2221 aggcctcgag gctgccgacg tcctcccagt agccctcggc gacgaagccg aacaggggcg
     2281 cgccctggtc gagcagcttg gggaagacgt cgccggcgaa gtcggacggc tccccctccg
     2341 ggatgtggtc gaacacctcg ggttccagca cgtagatgcc ggtgttgatg gtgtcggaga
     2401 agacctggcc ccagtggggc ttctcgagga agcgctcgat acggccgtcg tcggtggtga
     2461 tgacgatgcc gaactcgagc gggtcgggca tgcgcttgag caccaccgtg gccagggcgc
     2521 cgcggcggcg gtggaagctc acgacctcgt cgaggtcgat gtcggtcagg gcgtccccgg
     2581 agatgaccac gaaggtctcg ccgagcatct cggcggcgtt gcggacgctg ccggccgtgc
     2641 cgagcggggt cggctcggtg gcataggtca ggtccacctc catgtcggcg ccgtcgccga
     2701 agtagttgcg gacctggctg gccaggaact gcacggtgac cacgatgtcc ttgaacccgt
     2761 agtcccggat gtgccggacg atgtgctcca tgatgggccg gttgcccacc gggagcatgg
     2821 gcttcggctg gttggcggtg agggggcgca ggcgggtgcc ctccccgccg gccatgatca
     2881 ccgccttcac cggcatcccc cgcggggccg agaggacgag acgcttgtag ccatggagat
     2941 tcgttccctt gtctcttcgg cgccctgcca gggacgcatg gccgttcggg gcgaccggcc
     3001 ggtggggtcg ccggcacctg ggcgcccgga ccggggtcct ggtccggacg ggctcggcca
     3061 gacggcgggc ggctcctgtc ggcgctagga gccggcctcc gcccggtggc cgagccgttc
     3121 gagcaccatt ctgacgtaga ccgccccggc gaggtagtac atcaccaccc cggcccagag
     3181 cagggccagg ccgagcaggc gggcgaggtc ggccacggcc agcccggtcg cccccagagt
     3241 gaggatgggc agggccaaca gcagctccag ggtggccgcc ttgccgagga agatcacctc
     3301 cgggggctcg atcccccgcc gcttgaggat cggccagccg cagagcagca ccaggtccct
//
LOCUS       DASSWR010000131         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_33236,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000131 DASSWR010000000
VERSION     DASSWR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33236"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..559
                     /locus_tag="VFO47_02130"
     CDS             131..559
                     /locus_tag="VFO47_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8787531.1"
                     /translation="MHESIEREARGVVATCLGYQTRKLARAVTRLYNDRLRPLGLNLT
                     EMNLLAAIAAQGSVQPARLGRAMALEKSTLSRNSSRLVERGWVEVRDHPDGRGVLLAV
                     TARGNEMLLRAVPAWKQAQQQAQSLVAEALADLEAGEEAS"
     gene            674..1369
                     /locus_tag="VFO47_02135"
     CDS             674..1369
                     /locus_tag="VFO47_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787532.1"
                     /translation="MEHPLRRTLPGAVTYLVLAIWAGLVTAAIVAGVFAGDGASRLLL
                     PAAQAIPIAAVAIGYRVSPRFRSYLLTLDLRLVLAAQLWRVIGVAFLFALAFGQLPAG
                     FAVPAGIGDLATGIAALAVVLALGNGTLTRGRLYAFTALGVIDFLVADATGLTLEPPA
                     LAAWPLAIFPTLMIPFFAVLHLVAVLQSRHHWDDRVRHYAIPSQNQAPTRQLPWRSPA
                     SQTATPQTVAGAP"
     gene            complement(1417..2163)
                     /locus_tag="VFO47_02140"
     CDS             complement(1417..2163)
                     /locus_tag="VFO47_02140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8787533.1"
                     /translation="MSDLSGRTTVVVGASRGLGRGIATAFAEAGAPVVAVSRTAAAFP
                     EPANGAGTIQPEVADARDATAAASLLDRYEPEAVILVAGASPHMRPLQQQTWETFSVN
                     WQADVRIAFHWLREALLKPLRPGSRVVVFSSGAALNGSPLSGGYAGAKATQRLITGYA
                     QEEAKRAGLDITFTAVFPGFAPMTGVGGPAVQAYAAHAGQSVEEYLQQLGPRVTPEVA
                     GAALVGLVRADAANVAPEYLLTAAGLQKLP"
BASE COUNT          328 a          865 c          765 g          352 t
ORIGIN      
        1 cgacccggcc cggccactta ccatcctcgg cctggcacac gcctgaagcg aacatcgcac
       61 cgccctggcc aaggccctgg acgaagcgcg gctcttgaca agcacagcat gagcgttgta
      121 gatacaacct atgcacgaga gcatcgagag ggaagcacgc ggggtggtcg cgacctgcct
      181 tggctaccag acccggaagc tggccagggc ggtgacccgg ctctacaacg accgcttgcg
      241 gccactgggc ctcaacctca ccgagatgaa cctgctggcg gcgatcgccg cacaggggtc
      301 cgtgcagcca gccaggctcg ggcgagccat ggcgcttgag aagtcgacgc tcagccgcaa
      361 cagcagccgc ctggtggagc gggggtgggt ggaggtgcgc gatcatccgg atggtcgcgg
      421 cgtgcttctg gcggtgacgg cacgtgggaa cgagatgctg ctccgcgccg tccccgcatg
      481 gaagcaggcc cagcagcaag cccaatcgtt ggtcgccgag gcgctggccg acctcgaagc
      541 cggggaggag gcgtcgtgac ggggcgaccc cttttttttc gtcgtccagt tgtaactaca
      601 acctagcagt cggaggaaca ccatgactac caataccgac cccacccgac ccgacgccac
      661 cgcacctgct ggcatggaac acccactgcg ccggaccctg ccgggggccg tcacctacct
      721 cgtcctggcc atttgggccg gcctcgtgac ggcagcgatc gttgccggcg tctttgccgg
      781 cgacggcgcc tcccggctcc tcctgccagc ggcgcaggcc atccccatcg ccgccgtcgc
      841 gatcggctac cgcgtctcgc cacggttcag gagctacctg ctcaccctcg acctgcggct
      901 ggtgctggcg gcccagctgt ggcgcgtgat cggcgtcgcc ttcctcttcg cgctggcgtt
      961 cggccagctc cccgcaggct tcgccgtccc agccggcatc ggcgacctcg ccaccgggat
     1021 cgccgccttg gccgtcgtgc tggccctggg caacggcacc ctgacccgtg gtcgcctcta
     1081 cgccttcacc gcgctcggcg tgatcgactt cctcgtcgcg gacgccaccg ggctgaccct
     1141 cgaaccgccg gccctcgccg cctggccgct ggccatcttc cccaccctca tgatcccgtt
     1201 cttcgccgtg ctccacctcg tggccgtgct ccagagccgc catcactggg acgaccgcgt
     1261 ccgccactac gccatcccca gccaaaacca ggcgcctacc cgtcaattac cctggcgatc
     1321 accggccagc cagacggcga ccccgcagac ggtcgcgggg gcgccgtagc gtgacggtga
     1381 cccggtccct gctcgggttc ctcgccgcgc cggtgttcat ggcagcttct gcagcccggc
     1441 ggcagtgagc aggtactcgg gggcgacgtt ggcggcatct gcccgtacca gcccgaccag
     1501 ggcggcgccg gcgacctccg gggtgacccg cgggcccagc tgctggagat actcctccac
     1561 ggactggcca gcgtgggccg cgtacgcctg gaccgccggc ccaccgacgc cggtcatcgg
     1621 cgcgaacccg gggaacacgg cggtgaaggt gatgtccaga ccggcgcgct tcgcctcctc
     1681 ctgggcgtac ccggtgatca agcgctgggt ggccttggcg ccggcgtacc cgccgctgag
     1741 cggtgacccg ttcagcgcgg caccgctgct gaacacgacc accctgctgc cgggccgcag
     1801 cggcttgagc agcgcctcgc gcagccagtg gaacgcgatc cggacgtcgg cctgccagtt
     1861 gaccgagaac gtctcccacg tctgctgctg cagcggacgc atgtgcgggc ttgcgccggc
     1921 caccaggatg acggcctccg gctcatagcg gtcaaggagg ctggctgccg ccgtagcgtc
     1981 cctggcatcg gcgacctccg gctggatggt gccggcgccg ttggccggct caggaaacgc
     2041 cgccgcggtg cgggacacgg cgaccaccgg ggcgccggcc tcggcgaagg ccgtggcgat
     2101 cccgcggccc aggccgcggc tggcgccaac gacgaccgtg gtcctgcccg acaagtcact
     2161 catgatcgtc ctcgttcctt gcgcgtccat ggtttcggtg gcgcagcctt ccgcaggcgt
     2221 cacgggctgt tgtactcgtc atggcggcgg agccagagct ggaacccgtc gccctcgtcg
     2281 cggcccttcg gcgcccggtc taggatcggg
//
LOCUS       DASSWR010000132         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_372628,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000132 DASSWR010000000
VERSION     DASSWR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372628"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFO47_02145"
     CDS             <1..906
                     /locus_tag="VFO47_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787534.1"
                     /translation="AGLAALAGAAIPPVGACMRALWSRLLGDGGRLQAAFAVESTVQE
                     LIFVVGPPLVALLAAAFSPAAAVLGTGALLLAGTGVFAATPASRSWRPRRRAADWAGP
                     LRSPGIRAVLATIVLLATAFGTVEVTVVAGAEQLGSRTLAGPLLALWALGSLVGGLAF
                     GARASDRGPEQRLIGLLALVVAGIALLAVAAGLAQLAAGMVLAGLGIAPAIACLYLLV
                     DRLAPAGTVTEAFTWVTTAFATGFAVGNALGGTLVHRVGTDRSFLVAAGGIAAAALLA
                     RLRRSALAGTSAGMPEAADGLGRAG"
     gene            903..2801
                     /locus_tag="VFO47_02150"
     CDS             903..2801
                     /locus_tag="VFO47_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787535.1"
                     /translation="MMYHWYPRREPLPPGTKLSRDVVRRVWGFARDFRWQIAGYLLLL
                     GLSALAAVVPALLVRELLNTAIPQRSFSLVNLIGLTAVGVAVANAGLALGQRFLSSRI
                     GEGLIYRLRTSLFDHVQRQPLAFFTHTQTGALTSRLNSDVVGAQRALTGTLGSVFSNL
                     ISLVTTLVTMFLLNWRITLITVAVLPLFLLPARRVGRRLQAITRESMQLNAAMNTTMN
                     ERFNVAGALLVMLFGRRDRELAGFADKAASVRDIGVRSALYGQTFFVVLGLVGAVGTA
                     AVYWFGARLVLQGQLQAGDVVALAALVTQVYGPLTALTNARVDVMTAFVSFERVFEVL
                     DFESPIRDRPGAVPLDHPAGRIEFDHVGFRYPTADESTIASLTGGASAIGAAAGEPVL
                     HDVSFTVLPGELVALVGPSGAGKTTTAMLVARVYDVSEGAVRVDGTDVRDLELESLRG
                     AIGLVAQDPHLFHDTILANLRYARPDATLEEIEDACRAARILDLVESLPERWETVVGE
                     RGYRLSGGEKQRLAIARLLLADPAIVILDEATAHLDSEAGVHIQEALAAALRGRTSLV
                     IAHRLSTIVNADRILVIAGGRVVEQGRHAELLAAGGLYADLYNTQFGRAPSSPRADDR
                     PVSRAATA"
     gene            complement(2779..3045)
                     /locus_tag="VFO47_02155"
                     /pseudo
     CDS             complement(2779..3045)
                     /locus_tag="VFO47_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
BASE COUNT          329 a         1216 c         1055 g          445 t
ORIGIN      
        1 gccgggctgg ccgccctcgc cggggcggcg atcccgccgg tcggggcgtg catgcgggcg
       61 ctctggtccc gcctgctcgg cgacggcggc cggctccagg ccgcgttcgc ggtcgagtcg
      121 accgtgcagg agctgatctt cgtcgtcggg ccaccgctgg tcgccctcct ggccgccgcc
      181 ttctccccgg ccgcggccgt gctcggcacc ggcgcgctgc tgctggccgg gaccggggtg
      241 ttcgcggcga ccccggcgtc gcggtcctgg cgcccccgcc gccgcgccgc cgactgggcc
      301 gggccgctgc gcagccccgg catccgcgcc gtgctggcca ccatcgtgct gctggccacc
      361 gccttcggca cggtcgaggt gacggtggtg gcaggcgccg agcagctcgg ctcccgcacc
      421 ctggccggcc cgctgctcgc cctgtgggcg ctcggcagcc tggtcggcgg cctggccttc
      481 ggcgcccgcg cctccgaccg cggtcccgag cagcgcctga tcggcctgct cgccctcgtg
      541 gtggccggga tcgccctgct ggccgtggcc gccgggctgg cccagctggc cgcgggcatg
      601 gtcctggccg ggctcggcat cgccccggcc atcgcctgcc tgtacctgct ggtcgaccgg
      661 ctcgcccccg ccggcaccgt gaccgaggcc ttcacctggg tgacgaccgc cttcgcgacc
      721 gggttcgccg tcggcaacgc cctcggcggc accctggtcc accgggtcgg caccgaccgg
      781 tcgttcctgg tcgccgccgg cgggatcgcc gccgccgccc tgctggcccg gctgcgccgc
      841 tccgccctgg ccggcacctc ggccggaatg ccggaggccg ccgacgggtt ggggcgagca
      901 ggatgatgta ccactggtac ccgcgccggg agccgctgcc gcccggcacc aagctgtcgc
      961 gcgacgtggt ccggcgcgtc tgggggttcg cccgcgactt ccgctggcag atcgccggct
     1021 acctgctcct gctgggtctg tcggcgctgg ccgcggtcgt gcccgcgctg ctcgtgcgcg
     1081 agctgctcaa caccgccatc ccccagcgca gcttctccct ggtcaacctg atcggcctga
     1141 ccgccgtcgg ggtcgccgtg gccaacgccg gcctcgccct cggccagcgg ttcctgtcct
     1201 cccgcatcgg cgagggcctg atctaccggc tgcgcaccag cctgttcgac cacgtgcagc
     1261 gccagcccct ggctttcttc acccacaccc agaccggcgc cctcaccagc cgcctgaact
     1321 ccgacgtggt cggcgcccag cgggccctca ccggcaccct cgggtcggtc ttctccaacc
     1381 tcatcagcct ggtcacgacc ctggtgacca tgtttctgct gaactggcgc atcaccctga
     1441 tcacggtggc cgtgctgccc ctgttcctgc tcccggcccg gcgggtgggg cgccggctgc
     1501 aggccatcac gcgcgagtcg atgcagctca acgcggccat gaacaccacc atgaacgagc
     1561 gcttcaacgt cgccggggcg ctgctggtga tgctgttcgg ccgccgcgac cgggagctgg
     1621 ccggcttcgc cgacaaggcc gccagcgtcc gcgacatcgg agtccgctcc gccctctacg
     1681 gccagacctt cttcgtcgtt ctcggcctgg tcggggccgt cggcacggcc gccgtctact
     1741 ggttcggggc ccggctggtc cttcagggcc agctccaggc cggcgacgtg gtcgccctgg
     1801 ccgccctcgt cacccaggtc tacggccccc tcaccgccct gaccaacgcc cgggtggacg
     1861 tgatgaccgc gttcgtgtcc ttcgagcggg tcttcgaggt gctcgacttc gagagcccga
     1921 tccgcgaccg gccaggcgcg gtccccctgg accacccggc cgggcggatc gagttcgacc
     1981 acgtcggctt ccgctacccg acggccgacg agtcgaccat cgccagcctc accggcggcg
     2041 cctcggcgat cggggccgcc gccggcgagc cggtcctgca cgacgtcagc ttcaccgtcc
     2101 tccccggcga gctggtcgcc ctggtcggcc cgtcgggggc gggcaagacc accacggcca
     2161 tgctggtcgc cagggtctac gacgtcagcg agggcgccgt ccgggtcgac gggaccgacg
     2221 tgcgcgacct ggagctggag agcctgcggg gcgcgatcgg gctggtcgcc caggacccgc
     2281 acctgttcca cgacaccatc ctggccaacc tgcgctacgc ccggccggac gcgacccttg
     2341 aggagatcga ggacgcctgc cgcgccgcca ggatcctcga cctggtcgag agcctgcccg
     2401 agcgctggga gacggtggtc ggggagcgcg gctaccggct gtccggcggg gagaagcagc
     2461 gcctggccat cgcccggctg ctgctggccg acccggccat cgtgatcctc gacgaggcca
     2521 ccgcccacct cgactccgag gccggggtcc acatccagga ggccctggcg gccgccctcc
     2581 gcggccggac ctcgctggtc atcgcccacc ggctgtcgac catcgtcaac gccgaccgga
     2641 tcctggtcat cgccggcggc cgggtggtcg agcagggccg ccacgccgag ctgctggccg
     2701 ccggcggcct ctacgccgac ctctacaaca cccagttcgg ccgggccccg agctcgcccc
     2761 gggccgacga ccgcccggtc agccgggcgg caacggcctg aggtgccgtt cggagatctt
     2821 ccgcaccttc tcgacccgcc gccggtactt ctcgtcgtcg ccgagaaact cggggtcatc
     2881 cacgagcgca cgacctcgag ggcgagggcc gagccgatga tcctgccgcc gacgcacagg
     2941 acgttggtgt ccgagtgcga cctggccagg ccggcgcgga aggggtcctg gcagacggcg
     3001 gcggcgacac cgaagtcctt gtctcgtgcc cgtccgcctg gagga
//
LOCUS       DASSWR010000133         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_710504,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000133 DASSWR010000000
VERSION     DASSWR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710504"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1814)
                     /locus_tag="VFO47_02160"
     CDS             complement(135..1814)
                     /locus_tag="VFO47_02160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET8787536.1"
                     /translation="MIVGSFGLRRGRALVVAALAVLVLATGLGTVPRDASAPPGAMES
                     IRVIVQKRAAGDVSPELAVRRLGGQVTRALPIVAGFAATVPAAALDELARLAGVRAVT
                     PDSRVRVQAAAPGGSSIRSVYPQTIRADDVWQRGVTGRGVTVAVLDTGVASVPDLAGR
                     LVQVRNDLTGQTTPCKNLSGELDCNDRYGHGTFIAGLVAGSGAGSGGKWKGVAPDAKI
                     LSVKAAGADGSADVSNILAAIQWVVSFRDRYNIRVLNLSLGTDSTQDWRTDPLNYAVE
                     RAWAAGMTVVVAASNNGPGAGTITKPADDPWVVTVGATDDRGTAGLSDDQLPDFSSRG
                     PTAHGLAKPDVAAPGAHVVSLRAPGSTIDTRYPYYVDGAYRRGSGTSMATGVVSGAVA
                     LMLQANPGFTPDRVKHALTATARDAASSDPLAVGAGVVDVHAAAYSAPPGVANQGLAR
                     SNGMGSLGASRGSVQVKSDDPLGTVLGPVLGATLTAQLLLWNPTGYTGAPWLPSDWYV
                     STWEVHRWNRVSWYGNDWPGSKWHGSSWYGQGSGESYGSPLPGSAWYGAWE"
     gene            1997..>3416
                     /locus_tag="VFO47_02165"
     CDS             1997..>3416
                     /locus_tag="VFO47_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8787537.1"
                     /translation="MAITSDRATPRREDPPLVTGAAVYSDDLVPPGALHAVFVRAPLA
                     HGRILSIDPAPAAAMPGVAGVYTAADLDLPPMPAGDAPDVMARPVLATGTVRFLGEAV
                     AVVVAATRAQALDAAEAVEVDYDPLPVVIDPLAALADDAPRLFGEGGNLAKERRWPEP
                     SRALEGAEVVVRGRFVNQRLAAAPLEPNGAVAVPEPDGGGLLLYSPCQAPFWVRDDVA
                     DVLGLAPERVRVVVPAVGGAFGARIVTYPEQVALAALALRLGRPVRYVESRSETMLAM
                     THGRAQVQEVELAATREGRITGLRARLVADGGAYPGEAINMPSSTRYMASGVYRIARV
                     EVAWQCAATNTTPVAAYRGAGRPEATAMVERAVDLLAARLRMDPAELRWRNLIPARAF
                     PYTTATRARYDSGDYEGALDRVLEAAGYGELRAEQARRRERGDVRQLGIGLAVYVEVT
                     GFGSEYGAVEVEPDGRVTARTGV"
BASE COUNT          415 a         1247 c         1316 g          438 t
ORIGIN      
        1 cggcaggcgg ctctgggcca gcagcccgcc ggccaggccg actgcggcca gcgcggccgt
       61 ggccagcagc accgagacgg ggaaggcacc gtcccgcgcc ttccccgtgg ccccctcagc
      121 gacctgaccc acgcctactc ccaggcgccg taccatgccg atccgggcaa tggtgagccg
      181 tatgactcgc cgctgccttg cccgtaccag ctggatccgt gccacttgct gcccggccag
      241 tcgttgccgt accagctcac ccggttccag cggtggacct cccaggtgga gacgtaccag
      301 tcggagggga gccagggggc gccggtgtag ccggtggggt tccagaggag gagctgggcg
      361 gtcagggtgg cgccgaggac cgggccgagg acggtgccca gcgggtcgtc cgacttgacc
      421 tggaccgacc cgcggcttgc gccgaggctg cccatgccgt tggagcgggc caggccctgg
      481 ttggccacgc ccggcggggc cgagtaggcg gcggcgtgga cgtcgaccac cccggcgccg
      541 acggccagcg ggtcgctgga ggcggcgtcc ctggccgtgg ccgtcagggc gtgcttgacc
      601 cggtcggggg tgaagccggg gttggcctgg agcatcagcg cgaccgcgcc ggacaccacc
      661 ccggtggcca tggaggtgcc gctgccccgc cggtaggccc cgtcgacgta gtacgggtaa
      721 cgggtgtcga tggtgctgcc cggggcccgc agggagacga cgtgggcgcc gggggcggcg
      781 acgtccggct tggccagccc gtgggcggtc ggcccgcggc tggagaagtc gggcagctgg
      841 tcgtcgctga ggccggcggt gccgcggtcg tcggtggccc cgacggtgac cacccacggg
      901 tcgtcggccg gcttggtgat ggtcccggcg ccggggccgt tgttgctggc ggccacgacc
      961 acggtcatac cggccgccca ggcccgctcg accgcgtagt tgagcgggtc ggtgcgccag
     1021 tcctgggtgg agtcggtgcc caggctgagg ttgagcacgc ggatgttgta gcggtccctg
     1081 aaggagacca cccactggat ggcggccagg atgttgctga cgtcggcgga gccgtcggcc
     1141 ccggcggcct tgaccgacag gatcttggcg tcgggggcga cgcccttcca cttgccgccc
     1201 gagccggccc cgctgccggc caccagcccg gcgatgaagg tgccgtgccc gtagcggtcg
     1261 ttgcagtcca gttcgccgga gaggttcttg cagggcgtgg tctggccggt gaggtcgttg
     1321 cgcacctgga ccagccggcc ggccaggtcg ggcacgctgg ccacgccggt gtcgagcacg
     1381 gccacggtga ccccgcggcc ggtgacgccg cgctgccaga cgtcgtcggc cctgatcgtc
     1441 tgcgggtaga ccgagcggat actgctcccg ccgggagcgg cggcctggac ccggacccgg
     1501 ctgtcggggg tgaccgcccg gaccccggcc agtcgggcca gctcgtcgag ggcggccgcg
     1561 ggcacggtgg ccgcgaaccc ggcgacgatg ggcagggccc tggtgacctg cccgcccagc
     1621 cgccgcacgg ccagctccgg cgagacgtcg ccggccgcgc gcttctgcac gatgacccgg
     1681 atggactcca tggctcccgg cggggccgac gcgtcccgtg ggacggtccc cagcccggtg
     1741 gccagcacca acaccgccag cgcggcaacg accagcgccc ttccgcgccg cagcccgaac
     1801 gaaccgacga tcaccacagc cctcccctgg atgtgacacc cactctcaat gacgaccggg
     1861 acagactgtg cgtttagcct agtcggctta aacatcctcg acgaaaccta ctggcaagta
     1921 cgcaggcagc tgacaacggt tctcgcaaac cttcgtgtcc ggggttgctc ggccgccggt
     1981 gccgtaggct ctgcccatgg cgatcaccag cgaccgggcg acgccccggc gcgaggaccc
     2041 gcccctggtc accggggcgg cagtctacag cgacgacctg gtcccgccgg gggcgctgca
     2101 cgccgtgttc gtccgggcgc cgctggccca cggccggatc ctgtccatcg acccggcccc
     2161 ggcggccgcc atgcccgggg tggccggggt gtacaccgcc gccgacctgg atctgccgcc
     2221 gatgccggcc ggggacgcgc ccgacgtcat ggcccgcccg gtgctggcca ccggcacggt
     2281 ccgcttcctg ggcgaggcgg tggcggtggt ggtggccgcc accagggccc aggcgctgga
     2341 cgcggccgag gccgtcgagg tcgactacga cccgctgccg gtggtcatcg acccgctggc
     2401 cgccctggcc gacgacgccc cgcggctgtt cggcgagggc gggaacctgg ccaaggagcg
     2461 ccgctggccg gagccgtccc gggccctgga gggggccgag gtggtggtcc ggggccggtt
     2521 cgtcaaccag cggctggccg cggcccccct ggaacccaac ggcgccgtgg ccgtccccga
     2581 gcccgacggc ggcggcctcc tgctctactc gccctgccag gcgccgttct gggtgcgcga
     2641 cgacgtcgcc gacgtgctgg gcctggcccc cgagcgggtc cgggtggtcg tgccggcggt
     2701 gggcggggcc ttcggggcca ggatcgtcac ctatcccgag caggtcgcgc tggccgccct
     2761 ggccctgcgg ctgggccgcc cggtccgcta cgtcgagagc cgctcggaga cgatgctggc
     2821 catgacccac ggccgggccc aggtccagga ggtcgagctg gccgcgaccc gggaggggcg
     2881 catcaccggc ctgcgggccc ggctggtggc cgacggcggc gcctacccgg gcgaggccat
     2941 caacatgccg tcctcgaccc gctacatggc ctcgggcgtg taccggatcg cccgggtcga
     3001 ggtggcctgg cagtgcgcgg ccaccaacac caccccggtg gccgcctacc ggggcgccgg
     3061 ccggcccgag gccacggcca tggtcgagcg ggcggtcgac ctgctggcgg ccaggcttcg
     3121 catggacccg gccgagctgc ggtggcgcaa cctcatcccg gccagggcgt tcccgtacac
     3181 cacggccacc agggcccgct acgactcggg cgactacgag ggcgcgctcg accgggtcct
     3241 ggaggcggcc gggtacgggg agctccgggc cgagcaggcc aggcggcgtg agcgcggcga
     3301 cgtccgccag ctcggcatcg gcctggcggt gtacgtcgag gtgaccgggt tcggctccga
     3361 gtacggcgcg gtcgaggtcg agcccgacgg gcgggtcacg gccaggacgg gcgtct
//
LOCUS       DASSWR010000134         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1050747, whole genome shotgun sequence.
ACCESSION   DASSWR010000134 DASSWR010000000
VERSION     DASSWR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050747"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFO47_02170"
     CDS             <1..516
                     /locus_tag="VFO47_02170"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET8787538.1"
                     /translation="ANGLGNLASRVTAMVERYRDGVLPGPGPATDAEVAVQDAAERAY
                     ADAAAALEEVAYERALAAIWRFVGAANAYLSERKPWDLAKAGDDVALDTTLYTGAEAL
                     RIAALLTAPWLTKAAPALWAALGVPGELAEARLPGALAWGGLPRGARVERVGALFPRL
                     DAEGNVARRAG"
     gene            513..1058
                     /locus_tag="VFO47_02175"
     CDS             513..1058
                     /locus_tag="VFO47_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET8787539.1"
                     /translation="MTLAARGTQPVEVVDYDQDWPRAYAEERDRIAAAIGDTVLAIEH
                     VGGTAVPGLPAKPVIDLMVGVEDIERAGPAVAGLINLGYEYVPELESQLPDRRYFRLG
                     TPETHHVHMVPISSDYFEEHLLFRDWLRSHPQAAEEYGKLKRGLAGRFRLDRDAYRAG
                     KVPFIDMVVAAARREAGGQSS"
     gene            1068..>2408
                     /locus_tag="VFO47_02180"
     CDS             1068..>2408
                     /locus_tag="VFO47_02180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET8787540.1"
                     /translation="MTTTTSVPGRPRSTEVTAGRDRAPARSMLRAVGFTDDDFERPQV
                     GVASSWNEVTPCNIHLDRLAVASKDGVRLAGAVPLTFNTITVSDAIAMGHEGMRASLV
                     SREVIADSVELVMHAERLDGLVAIAGCDKSLPGMLMACARLDLPAVFVYGGTILPGHF
                     RGRDVTVQDMYEAVGAVAAGTMSEEDLDELERVACPTAGSCAGMYTANTMAAVSEAIG
                     MALPGSATPPAVSERRAEAARRTGEAVVNLLRLDIRPRQIMTRPAFLNAAAVVMATAG
                     STNAVLHLLAIANEAGVELTMDDFDAVSRRVPHIVDMRPGGRFVMADVDRVGGIPVVL
                     RELLEAGLLDGDVLTVTGRTMAENLEGAGEPDGEVVRRVATPIAREGGLAVLRGSLAP
                     DGSVVKIPGNEHLTFRGTARVFESEEDCFAAVTGGRVGKGDVLVIRNEGPRGGPG"
BASE COUNT          314 a          898 c          897 g          299 t
ORIGIN      
        1 gccaacgggc tcggcaacct ggccagccgg gtcacggcca tggtcgagcg ctaccgcgac
       61 ggggtcctgc ccggccccgg accggccacc gacgccgagg tcgcggtcca ggacgccgcc
      121 gagcgggcct acgccgacgc cgccgccgcc ctcgaggagg tggcctacga gcgggccctg
      181 gccgccatct ggcggttcgt gggcgcggcc aacgcctacc tgtcggagcg caagccctgg
      241 gacctggcca aggccggcga cgacgtcgcc ctcgacacca ccctgtacac cggggccgag
      301 gcgctgcgga tcgccgccct gctcaccgcc ccctggctga ccaaggccgc gccggcgctg
      361 tgggcggccc ttggcgtccc cggggagctg gccgaggcca ggctgcccgg ggcgctggcc
      421 tggggcgggc tgcccagggg ggcgagggtg gagcgggtcg gggcgctgtt cccgcggctg
      481 gacgccgagg gcaacgtggc caggcgggcg ggttgacctt ggccgcccgc gggacccagc
      541 ccgtcgaggt cgtggactac gaccaggact ggccccgcgc ctacgccgag gagcgcgacc
      601 ggatcgccgc cgccatcggc gacaccgtcc tggccatcga gcacgtcggc gggacggccg
      661 tgcccggcct gccggccaag ccggtgatcg acctgatggt cggggtggag gacatcgagc
      721 gggccgggcc ggccgtggcc ggcctgatca acctcggcta tgagtacgtg cccgagctcg
      781 agagccagct ccccgaccgc cgctacttcc ggctgggcac ccccgagacc caccacgtcc
      841 acatggtccc gatctcgagc gactacttcg aagagcacct gctgttccgg gactggctgc
      901 gcagccatcc gcaggcggcc gaggagtacg gcaagctcaa gcgcggcctg gccggccgct
      961 tccgcctcga ccgcgacgcc taccgggccg gcaaggtgcc gttcatcgac atggtggtcg
     1021 ccgccgcccg ccgcgaggcc ggcggtcaga gcagctagga gccatccatg accacaacga
     1081 ccagtgttcc cgggcgtccc cggtccaccg aggtcaccgc cgggcgcgac cgggccccgg
     1141 cccgctcgat gctgcgggcg gtcgggttca ccgacgacga cttcgagcgt ccccaggtcg
     1201 gggtggcgtc gtcctggaac gaggtcacgc cctgcaacat ccacctcgac cggcttgcgg
     1261 tcgcctccaa ggacggggtg cgcctggccg gggcggtccc gctgaccttc aacaccatca
     1321 ccgtgtccga cgccatcgcc atggggcacg agggcatgcg ggcctcgctg gtcagccggg
     1381 aggtcatcgc cgactcggtc gagctggtga tgcacgccga gcggctcgac ggcctggtcg
     1441 ccatcgccgg ctgcgacaag tcgctgccgg gcatgctgat ggcctgcgcc cgcctcgacc
     1501 tgccggccgt gttcgtctac ggcggcacca tcctgcccgg gcacttccgc ggccgcgacg
     1561 tcaccgtcca ggacatgtac gaggcggtcg gggccgtggc cgccggcacc atgtccgagg
     1621 aggacctgga cgagctggag cgggtggcct gcccgacggc cgggtcgtgc gccggcatgt
     1681 acacggccaa caccatggcg gcggtgtccg aggccatcgg catggccctg cccgggtcgg
     1741 ccaccccgcc ggcggtgtcg gagcgccgcg ccgaggcggc ccgccgcacc ggcgaggccg
     1801 tggtcaacct gctccggctc gacatccggc cgcgccagat catgaccagg ccggccttcc
     1861 tgaacgcggc cgcggtggtc atggccaccg ccgggtcgac caacgccgtc ctccacctgc
     1921 tggccatcgc caacgaggcc ggggtggagc tgaccatgga cgacttcgac gccgtgagcc
     1981 ggcgcgtgcc ccacatcgtc gacatgcgcc ccggcggccg cttcgtcatg gccgacgtcg
     2041 accgggtcgg gggcatcccg gtggtgctgc gcgagctgct ggaggccggg ctgctggacg
     2101 gcgacgtcct gaccgtcacc ggcaggacaa tggccgagaa cctggagggc gcgggcgagc
     2161 ccgacggcga ggtcgtccgc cgggtggcca cgccgatcgc ccgcgagggc ggcctggctg
     2221 tgctccgggg cagcctcgcc cccgacggct cggtggtgaa gatccccggc aacgagcacc
     2281 tgaccttccg gggcacggcc agggtgttcg agtccgagga ggactgcttc gccgccgtga
     2341 ccggcggccg ggtcggcaag ggggacgtgc tggtgatccg caacgagggc cccaggggcg
     2401 gccccggg
//
LOCUS       DASSWR010000135         5178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1333841, whole genome shotgun sequence.
ACCESSION   DASSWR010000135 DASSWR010000000
VERSION     DASSWR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333841"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..651
                     /locus_tag="VFO47_02185"
     CDS             157..651
                     /locus_tag="VFO47_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016579253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HET8787541.1"
                     /translation="MADTQGFEEFYTATVGRLLGQLFPVTGDLHEAEEVVQEAFARAS
                     IRWSRIRDYDVPEAWVRRVAMNLAADRHRRRHRQVAALHRAGPPPSVPPVSVEALALV
                     HALGTLPIRHRQAIVLHYLVDLPVEEVAATLGTSAGTVKSWLARGRRELAGRLGEPEE
                     VGNS"
     gene            648..1439
                     /locus_tag="VFO47_02190"
     CDS             648..1439
                     /locus_tag="VFO47_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787542.1"
                     /translation="MNDLREHLHELADAAARHGTTTGPAAAIRRGRQRRRRMAGAVAA
                     VLALVVAVGSGSLGRLAQPDLPATTPSTTLLPVPKLDVTTPEFAAGTRQAAAFDALVA
                     QVRRCPGGYTSPALIGYYRSAEYRRLVLVVGKRPGLGETTICWAAAVFDLDGKAERLD
                     AVRPASTAVPLTATNGGSSNVGVVQGQTAKEAALVRVRFRDQPALLELPVIQSWGSYV
                     VNLYIGLFPPGWVPVEVTAYDANGRQVAGCALDPSAATLGRCPGT"
     gene            complement(1443..1796)
                     /locus_tag="VFO47_02195"
     CDS             complement(1443..1796)
                     /locus_tag="VFO47_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET8787543.1"
                     /translation="MLFHCTHGRNDPERAIVPFIGANVAAASGQEVVVVLTIEGAWLC
                     KRGYAETVEADGFPELSELLASFVEAGGQIWGCSACTTPRGITEADLVSGAQIVGAAT
                     IVAAVAEGAVPVALG"
     gene            1896..3257
                     /locus_tag="VFO47_02200"
     CDS             1896..3257
                     /locus_tag="VFO47_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8787544.1"
                     /translation="MVVVGGDAAGMSAAAQARKRRGPEDLEIVAFERGRHTSYSACGL
                     PYYAGDIVKDWRDLVARTPEEHAEQDIEVRTRHEVDHVDLDARRVRARDLDGDRVVEE
                     PWDLLVMATGSEPLRPPIDGIDLPGVYGLSVLQDGIELRKAVDAGPERAVIVGGGYIG
                     LEAAEALVNRGIPTALVEAAPQVMNRIDPDMGELVSEAVRDIGVELHLEEEVEAIEGD
                     GRVRAVRTAERTLEADLVVLGLGTRPAVGLAESSGIELGQSGAIAVNPRMQTRHDGVW
                     AAGDCAEARHLVTGKPVSYHLGTIANKQGRVCGINIGGGYATFPGVLGTAVSKVCDVE
                     VARTGLSEAELRDLGLEWEQAAIDSTTRAGYFPGAAPIRTKVLADPRSGRLLGAQIVG
                     KEGAAKRIDVFAMAIWNGMTVQEMVNLDLSYAPPFSPVWDPVLIAVRKAAEQVERSLD
                     DHL"
     gene            3347..4534
                     /locus_tag="VFO47_02205"
     CDS             3347..4534
                     /locus_tag="VFO47_02205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HET8787545.1"
                     /translation="MPFDLQPIDVLLLVLLALAALSGWRRGFAMVLLGYLGLLVGLAL
                     GAWAATRAGLLVSPDSSARRLVTAVIVFFLVAAACHAVGNLLGIRVRSLLGGRWTSRA
                     DAIGGAVVATIVAAIAMWFVALTLSNVPLSPFSRAVHSSSVLRTIDTYAPRPPAALAQ
                     LRGLLDRSGFPEAFNTLRPPVVSGAPPEASRNPAVLRAANSTLQIRSRGCGGILFGSG
                     FPVANDLVVTNAHVVAGARRHEVVTNEDVRAGATVVYLDPSRDLALLSVPDLPVRPLR
                     LAGAPARPGQQAVVLGYPAGGRQDYIGAEIVQRVQPEAPDIYARPQGSRRDIYVLHAR
                     VRKGVSGGPVVDLRGRPLGVVFAASTVERSEGYALTNAEVRRAINRNAGNRRPVPVGG
                     CAV"
     gene            complement(4531..>5178)
                     /locus_tag="VFO47_02210"
     CDS             complement(4531..>5178)
                     /locus_tag="VFO47_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-binding protein"
                     /protein_id="HET8787546.1"
                     /translation="RRDLRHARAFGRAARAAGVDRVVYLSGLGDDREGLSEHLASRRA
                     VEDVLASTGPDLTVLRAAIVLGRGGASFEMLVQLVRRLPVMVCPRWVETASQPIALAD
                     AVGYLADAATTDATRAQRLEIGGPEVLTYREMMLRFARLEGRRRLIVSVPLFTPGLSS
                     HWIGLVTDVPAAVARPLAEGLRNRVVVGNGAARRLMPRDLATFEEACRAALATPA"
BASE COUNT          654 a         1957 c         1908 g          659 t
ORIGIN      
        1 cgttccggat gccttggcgt gtccatggtt cagcagcttc aggcgcgccc tggcggcgaa
       61 cccgttcccc ctgccattcg acggcgtcgc ctggcccgat tctgactgca tcttcgcccc
      121 acagcgctga atcgggtcga cacaaaggaa ctgtccgtgg ccgacaccca gggattcgag
      181 gagttctata cggccaccgt cggccggctg ctcggccagc tgttccccgt caccggcgac
      241 ctccacgagg ccgaggaggt cgtgcaggag gccttcgccc gggcctcgat ccgctggagc
      301 cggatccgcg actacgacgt gcccgaggcc tgggtgcgcc gggtggccat gaacctggcc
      361 gccgaccgcc accgccggcg ccaccggcag gtggccgccc tgcaccgggc cggcccgccg
      421 ccctcggtgc caccggtctc ggtggaggcc ttggcgctgg tccacgccct cggcacgctg
      481 cccatccggc atcggcaggc gatcgtgctc cactacctgg tggacctgcc ggtcgaggag
      541 gtggcggcca ccctcggcac cagcgccggt acggtcaaga gctggctggc ccggggccgg
      601 cgcgagctgg cgggacggct gggcgagccc gaggaggtgg gcaactcgtg aacgacctgc
      661 gcgagcacct ccacgagctg gccgacgcgg ccgcccgcca cgggaccacc accggcccgg
      721 cggccgccat ccgccgcggc cggcagcgcc gccggcggat ggcgggtgcc gtcgcggccg
      781 tcctcgccct cgtcgtggcc gtcggcagcg gctccctggg ccggctggcc cagccggacc
      841 tgccggcgac cacaccctcg acgaccctcc tgcccgtccc caagctggac gtcaccaccc
      901 cggagttcgc cgccggcaca cgccaggccg ccgccttcga cgccctggtc gcccaggtca
      961 ggcgctgccc cggcgggtac acctcaccgg ccctgatcgg ctactaccgg tcggccgagt
     1021 accgccgcct ggttctggtg gtcggcaagc gacccgggct cggggagacg accatctgct
     1081 gggcggcggc cgtgttcgac ctcgacggca aggccgagcg cctggacgcc gtccggcccg
     1141 cgtccaccgc cgtaccgctg acggccacga acggcgggtc cagcaacgtg ggggtcgtgc
     1201 aggggcagac ggccaaggag gcggccctcg ttcgggtccg gttccgcgac cagccggcgc
     1261 tcctggagct cccggtgatc cagtcctggg gcagctacgt ggtcaacctc tacattggcc
     1321 tcttccctcc gggctgggta cccgtcgagg tgaccgccta cgacgcgaac gggcgccagg
     1381 tcgccggctg cgccctcgac ccgtcggccg ccaccctggg gcggtgcccc ggcacgtgag
     1441 gctcagccca gggcgaccgg gaccgcgccc tcggcgacgg cggcgacgat ggtggcggca
     1501 cccacgatct gggcgccgga gaccaagtcg gcctcggtga tgccgcgggg ggtggtgcag
     1561 gcgctgcagc cccagatctg gccgccggcc tcgacgaacg aggccagcag ctcggacagc
     1621 tcggggaagc cgtcggcctc caccgtctcg gcgtagccgc gcttgcacag ccaggcgccc
     1681 tcgatggtca gcaccaccac cacctcctga ccggaggcgg cggccacgtt ggccccgatg
     1741 aacgggacga tcgcccgctc cgggtcgttg cgcccgtggg tgcagtggaa cagcatcctg
     1801 gtcaactggc tcggcctccc gggtagcggt cgctccgaca gggcagacta gcctgcggcg
     1861 gacgacgacg aggaggggtc gtggcgagga cgcgcatggt ggtggtcgga ggcgacgcgg
     1921 ccggcatgag cgccgccgcc caggcgcgca agcgccgtgg ccccgaggac ctggagatcg
     1981 tcgccttcga gcgggggcgg cacacctcct actcggcctg cgggctcccc tactacgccg
     2041 gcgacatcgt caaggactgg cgtgacctgg tcgcccgcac cccggaggag cacgccgagc
     2101 aggacatcga ggtccgcacc cggcacgagg tcgaccacgt cgacctggac gcccgccggg
     2161 tccgggcccg cgacctggac ggcgaccggg tcgtcgagga gccgtgggac ctgctggtga
     2221 tggccaccgg gtccgagccg ctgcggccgc ccatcgacgg catcgacctg ccgggggtgt
     2281 acgggctgtc ggtgctccag gacggcatcg agctgcgcaa ggcggtcgac gccggcccgg
     2341 agcgggcggt gatcgtcggc ggcggctaca tcggcctgga ggcggccgag gccctggtca
     2401 accggggcat ccccaccgcc ctggtcgagg cggcgccgca ggtgatgaac cggatcgatc
     2461 ccgacatggg cgagctcgtc tccgaggccg tcagggacat cggggtcgag ctccacctcg
     2521 aggaggaggt cgaggcgatc gagggcgacg ggcgcgtccg ggcggtccgg acggccgagc
     2581 gcaccctgga ggccgacctc gtggtgctcg gtctcggcac ccggccggcc gtggggctgg
     2641 ccgagtcctc cgggatcgag ctcggccaga gcggcgccat cgccgtcaac ccgcgcatgc
     2701 agacccggca cgacggggtg tgggcggccg gcgactgcgc cgaggcccgg cacctcgtca
     2761 ccggcaagcc ggtcagttac cacctgggca ccatcgccaa caagcagggc cgggtctgcg
     2821 gcatcaacat cggcggcggc tacgccacct tccccggcgt gctcggcacg gccgtgtcca
     2881 aggtgtgcga tgtcgaggtc gcccgcaccg ggctgtccga ggccgagctg cgcgacctgg
     2941 gcctggaatg ggagcaggcg gcgatcgact cgacgaccag ggccggctac ttcccggggg
     3001 ccgccccgat ccggaccaag gtgctggccg acccccgctc cgggcggctg ctcggcgccc
     3061 agatcgtcgg caaggagggg gcggccaagc gcatcgacgt gttcgccatg gccatctgga
     3121 acggcatgac cgtgcaggag atggtcaacc tcgacctttc ctacgcgccg ccgttctccc
     3181 cggtctggga cccggtgctg atcgccgtgc gcaaggccgc cgagcaggtc gagcggtcgc
     3241 tggacgacca cctgtaaggc ggcccccgcc gggcccggtg cagcccgtgc ttggtccgcc
     3301 gaccgattag gatgagacgg tcatcccgtc tgcggccgga acagcgatgc cattcgacct
     3361 gcaaccgatc gacgtgctcc tgctcgtcct gctcgccctg gccgcgctga gcggctggcg
     3421 gcgcgggttc gcgatggtgc tgctcggcta cctggggctg ctggtcgggc tggccctggg
     3481 ggcgtgggcg gccacccggg cggggctgct ggtctcgccc gacagctcgg cgcggcggct
     3541 ggtgaccgcg gtgatcgtgt tcttcctggt cgccgccgcc tgccacgccg tcggcaacct
     3601 gctcggcatc cgggtccgct ccctgctggg cgggcgctgg acgagccggg ccgacgccat
     3661 cggcggagcg gtggtggcca ccatcgtcgc cgccatcgcc atgtggttcg tcgccctgac
     3721 cctgagcaac gtgccgctgt cgccgttctc ccgggcggtg cactcctcca gcgtgctgcg
     3781 gaccatcgac acctacgccc cccggccccc ggcggccctg gcccagctgc ggggcctgct
     3841 cgaccgctcg ggcttcccgg aggcgttcaa caccctgcgg ccaccggtgg tctccggggc
     3901 gccgcccgag gcgtccagga acccggcggt gctgcgggcc gccaactcca ccctccagat
     3961 ccggagccgc ggctgcggcg ggatcctgtt cggcagcggc ttcccggtcg ccaacgacct
     4021 ggtggtcacc aacgcccacg tggtcgccgg ggcgcgccgc cacgaggtcg tgaccaacga
     4081 ggacgtccgg gccggggcca ccgtcgtcta cctcgacccg agccgcgacc tggccctgct
     4141 ctcggtcccc gacctgccgg tgcggccgct gcgcctggcc ggggcgccgg cccggccggg
     4201 ccagcaggcg gtggtgctcg gctacccggc cggcggccgc caggactaca tcggggccga
     4261 gatcgtgcag cgggtccagc cggaggcgcc ggacatctac gcccggccgc agggcagccg
     4321 ccgcgacatc tacgtgctcc acgcccgggt gcgcaagggg gtcagcggcg gcccggtggt
     4381 cgacctgcgc ggccggccgc tcggggtggt gttcgccgcc tcgacggtcg agcgcagcga
     4441 gggctacgcc ctcaccaacg ccgaggtccg ccgggccatc aaccgcaacg ccggcaaccg
     4501 ccgccccgtg ccggtcggcg gctgcgcggt ctaggcaggg gtggccaagg cagcccggca
     4561 ggcctcctcg aaggtggcca ggtcgcgggg catgaggcgg cgggcagcgc cgttgccgac
     4621 cacgacccgg ttgcgcagcc cctcggccag gggccgggcc accgccgccg gcacgtcggt
     4681 gaccagcccg atccagtgcg acgacagccc cggggtgaac aggggcacgc tgacgatcag
     4741 ccgccgccgg ccctccaggc gggcgaaccg cagcatcatc tcccggtagg tcaggacctc
     4801 gggcccgccg atctccagcc gctgggcgcg ggtcgcgtcg gtggtggcgg cgtcggccag
     4861 gtagccgacg gcgtcggcga gggcgatggg ctggctggcc gtctccaccc agcgggggca
     4921 gaccatcacc ggcagccgcc ggacgagctg gaccagcatc tcgaagctgg ccccgccccg
     4981 gccgagcacg atcgccgccc gcagcacggt caggtcgggc ccggtcgagg ccagcacgtc
     5041 ctcgaccgcc cggcggctgg ccaggtgctc actgagcccc tcgcggtcgt cgcccaggcc
     5101 ggacaggtac accacccggt cgaccccggc cgccctggcc gcccggccga aggcgcgggc
     5161 gtggcgcagg tcgcggcg
//
LOCUS       DASSWR010000136         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_400788,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000136 DASSWR010000000
VERSION     DASSWR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400788"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3375)
                     /locus_tag="VFO47_02215"
     CDS             complement(<1..>3375)
                     /locus_tag="VFO47_02215"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET8787547.1"
                     /translation="HSIVEIVTDDMPFLVDSVAMELNRHGLVIHLPIHPVIVVRRDEA
                     GGLVEVLPPDATDGIGESYLHFEVDRQSEPEVLDQLRADIERVLEDVKASTEDWAEMR
                     TQVNKILEEFDEHPPPVDGAELAEARALLEWIDDHHFTFLGYRSYDLVTEDGADVLRP
                     VPGTGLGILRQDQGKPASGSFAKLPPEARRLARERHVLILTKANSHSTVHRPSYLDYI
                     GVKRFDDQGEVVGERRFLGLYTSAAYNRNPRDIPVLRRKVTKVLERAGLPRYSHDWKS
                     LLNILETFPRDELFQISDDQLFETAMGILRLEERRRVRLFMWHDDYGRFVSCLVYVPR
                     DRYTTTVRRRIEVVLNDAFPGASFDFQVWLSESVLARLHFIIRVPPGQMPTYDARELE
                     AKLATVARSWNDELHALLLELHGEEEGNDLFARYGEAFPAAYLDDFPARNAVYDIQRM
                     EDLADRGGLNMNLYRPLEATPGTLRLKLFWADRPIPLSDAVPMLEDMGVEVVEERPYE
                     IHPPGRGTVWIHDFGVTYTVPEGDVDLGRVRDVFQDTFAAILRGEAESDGFNRLVLRA
                     RLTWRQIVVLRAYCKYLRQTRMTFSQEYMEQALAGNPAIARWLIELFQGRFDPTQADG
                     AESRVEELRERLAEAIDQVANLDEDRILRGFLGMVEATLRTNYYQVDEGGQPKGYLSF
                     KLDPAQVPSLPKPRPMFEVFVYSPRTEAVHLRGGRVARGGIRWSDRREDFRTEILGLM
                     KAQMVKNAVIVPVGAKGGFVVKQPPAEGGREALMDEVVACYSTMMRGLLDITDNRVGG
                     KVVPPPDVVRYDDDDPYLVVAADKGTATFSDLANSIAQEYGFWLDDAFASGGSAGYDH
                     KKMGITARGAWESVKQLFRETGIDTQSTDFTVVGIGDMSGDVFGNGMLLSRHIKLVGA
                     FNHLHVFLDPDPDPEAGFAERERLFALPRSSWSDYDTARISEGGGVWPRTAKSIPLSE
                     PVRRVLDLSADALTPNELINAMLKAPVDLLWNGGIGTYVKAAREAHADVGDRTNDAVR
                     VNGGELQAKVVGEGGNLGFTQLGRVEYALHGGRINTDFIDNSGGVDCSDHEVNIKILL
                     NQVVSDGDMTRKQRDKLLADMTDDVATAVLQDNYRQV"
BASE COUNT          474 a         1165 c         1197 g          539 t
ORIGIN      
        1 acctgccggt agttgtcctg cagcaccgcc gtggccacgt cgtcggtcat gtcggccagc
       61 agcttgtcgc gctgcttgcg ggtcatgtcg ccgtcgctga ccacctggtt gagcaggatc
      121 ttgatgttga cctcgtggtc ggagcagtcg acaccgccgg agttgtcgat gaagtcggtg
      181 ttgatgcggc cgccatggag ggcgtactcg acccggccga gctgggtgaa gccgaggttg
      241 ccgccctcgc cgacgacctt ggcctgcagc tcgcccccgt tgacccggac ggcgtcgttg
      301 gtccggtcgc cgacgtcggc gtgggcctcg cgggccgcct tcacgtaggt gccgatgccg
      361 ccgttccaga gcaggtccac gggggccttg agcatggcgt tgatcagctc gttgggggtg
      421 agcgcgtcgg cgctgaggtc caggacccgc cggaccggct ccgacagcgg gatcgacttg
      481 gccgtgcgcg gccagacccc gccgccctcg gagatcctgg ccgtgtcgta gtccgaccag
      541 gaggagcggg ggagggcgaa caggcgctcc cgctcggcga agccggcctc cgggtcgggg
      601 tcggggtcga ggaacacgtg caggtggttg aaggccccga ccagcttgat gtggcggctg
      661 aggagcatgc cgttgccgaa cacgtcgccg gacatgtcgc cgatgccgac gacggtgaag
      721 tcggtcgact gggtgtcgat cccggtctcg cggaacagct gcttgaccga ctcccaggcg
      781 ccccgggcgg tgatccccat cttcttgtgg tcgtagccgg ccgacccgcc ggaggcgaag
      841 gcgtcgtcga gccagaagcc gtactcctgg gcgatcgagt tggccaggtc ggagaaggtg
      901 gcggtgccct tgtcggccgc caccaccagg tacgggtcgt cgtcgtcgta gcggaccacg
      961 tccggcgggg ggacgacctt gccgccgacc cggttgtcgg tgatgtcgag caggccccgc
     1021 atcatggtcg agtagcaggc caccacctcg tccatcaggg cctcgcggcc gccctcggcc
     1081 ggcggctgct tgaccacgaa cccgcccttg gcccccaccg gcacgatcac ggcgttcttg
     1141 accatctgcg ccttcatcag gccgaggatc tcggtgcgga agtcctcccg ccggtccgac
     1201 cagcggatcc cgcccctggc cacccggccc ccgcgcaggt gcacggcctc ggtgcggggc
     1261 gagtacacga acacctcgaa catcggccgc ggcttgggca gcgacggcac ctgggccggg
     1321 tcgagcttga acgacaggta ccccttgggc tggccgccct cgtccacctg gtagtagttg
     1381 gtgcgcaggg tcgcctcgac catgccgagg aagccgcgca ggatccggtc ctcgtccagg
     1441 ttggccacct ggtcgatcgc ctcggccagc cgctcccgca gctcctccac ccggctctcg
     1501 gcgccgtcgg cctgggtcgg gtcgaaccgg ccctggaaca gctcaatcag ccagcgggcg
     1561 atcgccgggt tcccggccag ggcctgctcc atgtactcct ggctgaaggt catccgggtc
     1621 tggcgcaggt acttgcagta ggcgcgcagc accacgatct gccgccaggt gagccgggcc
     1681 cgcaggacca gccggttgaa gccgtcgctc tcggcctcgc cgcggaggat ggccgcgaag
     1741 gtgtcctgga acacgtcgcg gacccggccc aggtcgacgt cgccctcggg aacggtgtag
     1801 gtcacgccga agtcgtggat ccagaccgtg ccccggccgg gtgggtggat ctcgtagggc
     1861 cgctcctcga ccacctcgac gcccatgtcc tccagcatcg gcaccgcgtc cgacagcggg
     1921 atcggccggt cggcccagaa cagcttgagc cgcagcgttc ccggcgtcgc ctccagcggc
     1981 cggtacaggt tcatgttcag gccgccgcgg tcggccaggt cctccatccg ctggatgtca
     2041 tagacggcgt tgcgggccgg gaagtcgtcc aggtaggcgg ccgggaacgc ctcgccgtag
     2101 cgggcgaaca ggtcgttgcc ctcctcctca ccgtgcagct ccagcagcag ggcgtgcagc
     2161 tcgtcgttcc aggagcgggc caccgtggcc agcttggcct ccagctccct ggcgtcgtag
     2221 gtcggcatct gccccggcgg gacccggatg atgaagtgca gccgggccag gaccgactcc
     2281 gacagccaca cctggaagtc gaagctcgcc cccgggaagg cgtcgttgag gaccacctcg
     2341 atgcgccggc gcacggtggt cgtgtagcgg tcgcggggca cgtagaccag gcaggagacg
     2401 aagcggccgt agtcgtcgtg ccacatgaac agccgcaccc ggcggcgctc ctccagccgc
     2461 aggatgccca tggccgtctc gaacagctgg tcgtcggaga tctggaacag ctcgtcgcgc
     2521 gggaacgtct ccaggatgtt cagcagcgac ttccagtcgt ggctgtaccg gggcagcccg
     2581 gcccgctcca gcaccttggt caccttgcgg cgcagcaccg ggatgtcgcg cgggttccgg
     2641 ttgtaggcgg ccgaggtgta caggccgagg aagcgccgct cacccaccac ctcgccctgg
     2701 tcgtcgaagc gcttgacgcc gatgtagtcc aggtacgacg gccggtgcac cgtcgagtgc
     2761 gagttcgcct tggtcaggat gagcacgtgc cgctcgcggg ccagccgcct ggcctccggc
     2821 ggcagcttgg cgaagctgcc cgaggccggc ttgccctggt cctggcggag gatgcccagg
     2881 ccggtgcctg gcaccgggcg gagcacgtcg gcgccgtcct cggtgaccag gtcgtaggag
     2941 cggtagccga ggaaggtgaa gtggtggtcg tcgatccact ccagcagcgc cctggcctcg
     3001 gccagctcgg ccccgtcgac cgggggcggg tgctcgtcga actcctccag gatcttgttg
     3061 acctgggtgc gcatctcggc ccagtcctcg gtggaggcct tgacgtcctc caggacccgc
     3121 tcgatgtcgg cccgcagctg gtccagcacc tccggctcgc tctggcggtc gacctcgaag
     3181 tggaggtacg actcgccgat gccgtcggtg gcgtcgggcg gcagcacctc gaccagcccg
     3241 ccggcctcgt cgcggcggac cacgatcacc gggtggatgg gcaggtggat gaccaggccg
     3301 tggcggttca gctccatggc caccgagtcg accaggaacg gcatgtcgtc ggtgacgatc
     3361 tccacgatcg agtgc
//
LOCUS       DASSWR010000137         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_994657,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000137 DASSWR010000000
VERSION     DASSWR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994657"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFO47_02220"
     CDS             <1..315
                     /locus_tag="VFO47_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787548.1"
                     /translation="VELATDIPARPTPAIETIAYFCAAELLANAAKHSHAGRVTVQAD
                     GRHGTLVLQVGDDGVGGADPARGSGLAGLAQRVSTVDGRLRIASPRGGPTLISVHLPL
                     RT"
     gene            329..973
                     /locus_tag="VFO47_02225"
     CDS             329..973
                     /locus_tag="VFO47_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8787549.1"
                     /translation="MRVVIAEDAAVFREGLVRLLEDRGHEVCAAVGDGEELLAATAAH
                     RPDVAVVDIRMPPTHTDEGLRAALQLRRDHPGTGVLVFSQYIETRYAARLLQGNAAGV
                     GYLLKDRVADVAEFADALARVAAGGTALDPEVVSQLLGAGRHARGLAALTPREAEVLS
                     LMAEGRSNAGIAAVLVISAGVVEKHVASIFGKLGLPPAEGDNRRVLAVLRYLES"
     gene            1166..2080
                     /locus_tag="VFO47_02230"
     CDS             1166..2080
                     /locus_tag="VFO47_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787550.1"
                     /translation="MPGTAARPDRRRLLRVVGVGVAGIAVGVGVTLAAAGVGGGAGRA
                     ASVQRLDLPAGVAAPAAATPDGAGKAAPVRAGSARAAVRRFLQAGADGDFAVAYDLLD
                     QAGRGRYPSLARFTRAQADRAEVTAVRVGRERRTGDDRTDVTATRSHPAAIDPFAGLV
                     PARTVEVWRASRQDGRWRVAADPVSVRADLPADDRAPEAVTAWVERLLGCDRQGAAGL
                     QAGAGLYGPADLAELPCRERGRWTVAAPEGFDAAVEPEAYVAAFGPEVGSWARLVPVQ
                     GPRSRFSVVVGPLGDAWRVLGTDPVNAR"
     gene            2140..>3150
                     /locus_tag="VFO47_02235"
     CDS             2140..>3150
                     /locus_tag="VFO47_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET8787551.1"
                     /translation="MSKFRKLMTVGGLAATLALVSVVGTLAPSGASSHREALQIAQDP
                     VADNTDTYAFVSPDKPDTVTLVGNWIPLEEPAGGPNFHNFGDDVKYTLNVDNNGDAVD
                     DIVWEFRFSTKVQNGNTFLYNTGPITSLNDPDFNIRQTYSVAKVEKGRRTVLASGLPV
                     PPANIGPRSTPNYDQLASAAVAGLSDGSKVFAGPRDDPFFVDLGSVFDLAGLRPFNQA
                     HLIPRPTEAGVDGVSGYNTHSIVLQVPIKDLTGDKKALTGSDDPDAVIGVYAASWRRQ
                     TKVLSPSGGDPLSLGQWVQVSRLGMPLVNEVVVGLPDKDRFNASEPKHDAQFATYVTH
                     PSL"
BASE COUNT          408 a         1265 c         1095 g          382 t
ORIGIN      
        1 gtcgagctgg ccaccgacat cccggcccgg cccaccccgg cgatcgagac gatcgcctac
       61 ttctgcgccg ccgagctgct ggccaacgcg gccaagcaca gccacgccgg ccgggtcacc
      121 gtccaggccg acgggcgcca cggcacgctg gtcctccagg tcggcgacga cggcgtcggc
      181 ggcgccgacc cggcccgcgg cagcggcctg gccggcctgg cccagcgggt cagcaccgtc
      241 gacggccggc tgcggatcgc cagcccccgc ggcgggccga ccctgatcag cgtccacctg
      301 cccctgcgga catgacgacg ggccggccat gcgcgtggtg atcgccgagg acgccgccgt
      361 cttccgggaa ggccttgtcc gcctgctcga ggaccgtggc cacgaggtgt gcgccgccgt
      421 cggcgacggc gaggagctcc tggccgccac ggccgcgcac cgccccgacg tcgcggtggt
      481 ggacatccgc atgccgccga cccacaccga cgaggggctg cgcgccgccc tgcagctccg
      541 ccgcgaccac ccggggaccg gggtgctggt cttctcccag tacatcgaga ccaggtacgc
      601 ggccaggctg ctccagggca acgccgccgg cgtcggctac ctgctcaagg accgggtcgc
      661 cgacgtggcc gagttcgccg acgccctggc ccgggtcgcg gccggcggca ccgccctcga
      721 tcccgaggtc gtcagccagc ttctcggcgc cggccgccac gccaggggcc tggccgccct
      781 gaccccgcgg gaggcggagg tgttgtccct gatggccgag ggccgctcca acgccggcat
      841 cgccgccgtc ctcgtcatct ccgccggcgt ggtcgagaag cacgtggcca gcatcttcgg
      901 caagctcggc ctgcccccgg ccgagggcga caaccggcgc gtcctggcgg tcctccgcta
      961 cctggagtcc tgagcggcgt actccagcct acggccaatg gcctaggtgg aattacgccg
     1021 gcgcggagca aacgcttccg ccgccgggaa cgaacaggac acgaagacaa gcaaccagcc
     1081 gggtggtcac cgctcggcgg acgcgcccgg gcccggcgcc tccctgttcc caaccgctcg
     1141 tccctcgagc cgaggaggtc ccaccgtgcc cggaactgcc gcacgccccg accgccgccg
     1201 cctgctccgc gtggtcgggg tcggcgtggc cgggatcgcc gtcggggtcg gggtcaccct
     1261 ggccgccgcc ggggtcgggg gcggcgccgg ccgcgccgcc tcggtgcagc gcctcgacct
     1321 cccggccggc gtcgccgccc cggcggccgc gaccccggac ggggccggca aggccgcgcc
     1381 cgtgcgcgcc gggtcggccc gggcggccgt gcggcgcttc ctccaggccg gggccgacgg
     1441 cgacttcgcc gtcgcctacg acctgctcga ccaggcaggc cgcgggcgct acccgtcgct
     1501 ggcccgcttc acccgcgccc aggccgaccg ggccgaggtc acggccgtgc gggtcgggcg
     1561 cgagcgccgg accggagacg accgcaccga cgtcaccgcc acccggtccc acccggcggc
     1621 catcgacccg ttcgccgggc tggtcccggc gcgcaccgtg gaggtgtggc gggccagccg
     1681 ccaggacggc cgctggcggg tcgccgccga cccggtgtcg gtccgggccg acctgcccgc
     1741 cgacgaccgc gcccccgagg cggtcaccgc ctgggtcgag cggctgctcg gctgcgaccg
     1801 ccagggggcg gccggcctgc aggccggggc cgggctgtac ggcccggccg acctggccga
     1861 gctgccctgc cgggagcgcg gccgctggac ggtggccgcc cccgagggct tcgacgccgc
     1921 cgtcgagccg gaggcctatg tggccgcctt cggtcccgag gtcggctcct gggcccggct
     1981 ggtgccggtc cagggcccgc gctcgcgctt ctccgtggtc gtcggcccgc tgggcgacgc
     2041 ctggcgtgtg ctcggcaccg atcccgtcaa cgcccgctga gaggaggcct ccgagaaccc
     2101 gacgtgctgt cccatccgtc ctcccgagga ggaacgcagt tgtccaagtt ccgcaagctc
     2161 atgacggtgg ggggtctggc cgccacgctg gcgctggtct ccgtcgtggg caccctggcg
     2221 ccgtcggggg cgtcaagcca ccgcgaggcc ctgcagatcg cgcaggaccc ggtggccgac
     2281 aacaccgaca cctacgcctt cgtcagcccc gacaagcccg acacggtcac cctggtcggg
     2341 aactggatcc cgctggagga gccggccggc gggcccaact tccacaactt cggcgacgac
     2401 gtgaagtaca cgctcaacgt cgacaacaac ggcgacgccg tcgacgacat cgtgtgggag
     2461 ttccgcttct ccaccaaggt ccagaacggc aacaccttcc tgtacaacac cggccccatc
     2521 acctcgctca acgaccccga cttcaacatc cgccagacct actcggtggc caaggtcgag
     2581 aagggccgcc gcacggtcct cgcctccggg ctgcccgtgc cgccggccaa catcggcccg
     2641 cggtcgacgc ccaactacga ccagctggcc tccgcggccg tcgccggcct gagcgacggc
     2701 agcaaggtct tcgccggtcc ccgcgacgac ccgttcttcg tcgacctggg ctcggtgttc
     2761 gacctggccg gcctgcggcc gttcaaccag gcccacctga tcccgcgtcc caccgaggcc
     2821 ggggtcgacg gggtcagcgg ctacaacacc cactcgatcg tcctccaggt ccccatcaag
     2881 gacctcaccg gggacaagaa ggccctcacc gggtccgacg accctgacgc ggtcatcggc
     2941 gtgtacgccg cctcctggcg ccgccagacc aaggtcctgt cgcccagcgg cggcgacccg
     3001 ctcagcctcg gccagtgggt ccaggtgtcg cgcctcggca tgccgctggt gaacgaggtc
     3061 gtggtcgggc tgcccgacaa ggaccgcttc aacgccagcg agccgaagca cgacgcgcag
     3121 ttcgccacct acgtcacgca cccgagcctg
//
LOCUS       DASSWR010000138         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_937928,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000138 DASSWR010000000
VERSION     DASSWR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937928"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFO47_02240"
     CDS             complement(<1..385)
                     /locus_tag="VFO47_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8787552.1"
                     /translation="MIALVTGAAGFVGSHLSAALLDDGAEVLGVDAFTDYYSRLRKEQ
                     NLAHLRGRPGFSFLEDDLSEAPLAPLLDGVDCVFHLAGQPGVRASWGPDFVQYVRHNI
                     AATQRLLEASMLHPLRKFVFSSSSSV"
     gene            complement(382..1671)
                     /locus_tag="VFO47_02245"
     CDS             complement(382..1671)
                     /locus_tag="VFO47_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET8787553.1"
                     /translation="MTVQAPRSDRPQVSARRRTGDGDGLARAGVVLGIATLPILQPAF
                     VNNVAPADLGLVAGIAAVLVWAGMTRQRLRLPYVAGMGVMVIAGTIAAAFGDLPWMGA
                     VTIVQDLYLLAWAAALANFGRTAARAGFIVDVWCLSAAAWSIALVVLYGPSTIGGGDD
                     AERAGFTFGEQNAAGLWFALSLLVMLAARRPRRWRWRAPALGAVSLGLLLTGSLGAIS
                     GLFAGLAVALVLQVRARRGPDTAIAFSLALLLLVGSVGLLAQQGELIERASQSPNPLI
                     RNSIGRGSDSESERILVNQQTFGLFQTSGFLGRGPVTTEHTLRQQEAAYPHEAHNDWV
                     AALVERGVLGFAGILLLALEVIVLALTIWNPSRLKPDFAEVLPAPAYLVAGFILVALY
                     SLTHEVLHERPLWTLFGTAAALGLWGKANRWSLGGST"
     gene            complement(1680..>2024)
                     /locus_tag="VFO47_02250"
     CDS             complement(1680..>2024)
                     /locus_tag="VFO47_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787554.1"
                     /translation="HEVFNVGSDEQNHRLVDVAQLISELVPGVRVRFEEAAAEEANYR
                     VSFAHIRSALGFTPRHSLADGIAEVKAAVERGLVGNYTDAKYSNFKAMTSGQAARALD
                     DGRQDVPTAAVG"
BASE COUNT          308 a          710 c          761 g          245 t
ORIGIN      
        1 agaccgacga gctggagctg aagacgaact tgcgcagcgg gtggagcatg ctcgcctcga
       61 gcaggcgctg ggtggcggcg atgttgtggc gcacgtactg gacgaagtcc ggtccccagg
      121 aggcgcgcac gcccggctgc ccggcgaggt ggaacacgca gtcgacgccg tccagcagcg
      181 gcgccagggg cgcctcggag aggtcgtcct cgaggaagct gaacccgggc cgcccgcgaa
      241 ggtgggcgag gttctgctcc ttgcgcagac ggctgtagta gtcggtgaag gcgtcgacgc
      301 ccagcacttc ggcgccgtcg tcgagcagcg ccgcactcag atgcgaccca acgaacccgg
      361 cagctcccgt caccaacgcg atcatgtcga cccccccagg gaccagcggt tggccttgcc
      421 ccacagcccg agggccgcgg ccgtgccgaa caacgtccac agcggccgct cgtgcaggac
      481 ctcgtgggtg aggctgtaca gggcgaccag gatgaatccc gccaccaggt aggcgggcgc
      541 cggcaggacc tcggcgaagt ccggcttgag ccggctcggg ttccagatgg tgagcgcgag
      601 cacgatcacc tcgagggcga gcaggaggat gccggcgaac ccgaggacgc cccgctccac
      661 cagggccgcc acccagtcgt tgtgggcctc gtgcgggtag gcggcctcct gctggcgcag
      721 ggtgtgctcg gtggtgacgg gccctcggcc caggaacccg gaggtctgga acaggccgaa
      781 ggtctgctgg ttgaccagga tgcgctcgga ctcgctgtcg gaccctcggc cgatcgagtt
      841 gcggatcagg gggttcgggc tctggctggc ccgctcgatc agctcgccct gctgggccag
      901 cagccccacc gagcccacca gcagcagcag ggcgagcgag aaggcgatgg ccgtgtccgg
      961 tccgcgccgg gcccggacct ggaggaccag ggcgacggcc aggccggcga agagcccgct
     1021 gatggcgccc agcgaaccgg tgagcagcag gccgagggag accgcgccga gggcgggcgc
     1081 ccgccagcgc caccgccgcg ggcgccgggc ggccagcatg accagcaggg agagggcgaa
     1141 ccagaggccg gcggcgttct gctcgccgaa ggtgaagccg gcccgctcgg cgtcgtcccc
     1201 gcccccgatg gtgctcgggc cgtacagcac caccagggcg atggaccagg cggccgcgct
     1261 caggcaccag acgtccacga tgaacccggc ccgggccgcc gtgcggccga agttggccag
     1321 cgccgccgcc caggccagca ggtacaggtc ctgcacgatg gtgaccgcgc ccatccaggg
     1381 gaggtcgccg aaggcggcgg cgatggtccc ggcgataacc atgacgccca tcccggccac
     1441 gtacggcagc cgcaggcgct gcctggtcat gccggcccac accagcaccg ccgcgatgcc
     1501 cgcgaccagc cccaggtcgg ccggggcgac gttgttgacg aaggcgggct ggaggatggg
     1561 cagcgtggcg atgccgagca ccaccccggc ccgggccagc ccgtcgccgt cgccggtacg
     1621 gcgacgggcg ctgacctgtg gccggtccga tcgcggtgcc tggacggtca tgtcccagct
     1681 cagcccacgg cggccgtcgg cacgtcctgc cggccgtcgt ccagggcgcg ggcggcctgg
     1741 ccgctggtca tcgccttgaa gttgctgtac ttggcgtcgg tgtagttgcc gaccagcccg
     1801 cgctcgacgg ccgccttgac ctcggcgatc ccgtcggcga gggagtggcg cggggtgaac
     1861 ccgagggcgg agcggatgtg ggcgaaggag acccggtagt tggcctcctc ggcggccgcc
     1921 tcctcgaacc gcacccggac ccccgggacc agctccgaga tgagctgggc cacgtcgacc
     1981 agccggtggt tctgctcgtc ggagccgacg ttgaagacct cgtg
//
LOCUS       DASSWR010000139         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_881517,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000139 DASSWR010000000
VERSION     DASSWR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881517"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /gene="hypF"
                     /locus_tag="VFO47_02255"
     CDS             <1..2120
                     /gene="hypF"
                     /locus_tag="VFO47_02255"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017611781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET8787555.1"
                     /translation="REDAPPLAIVERVLTEPVEPLPGTGFRIVESARGGRPSALVSAD
                     IATCDDCLRELFNPADRRHRYPFLNCTNCGPRFTIVTGIPYDRPNTTMAGFVMCPACR
                     REYDDPADRRFHAQPVCCPACGPALALLDREGRAAGDDPLEAAAARLRDGAVVAVKGL
                     GGYHLAADAASEPAVAALRARKHREDKPFAVMAADLDGARALCLVDRAEEAMLASPRR
                     PIVLLRRRPDLRAGVAGPVAPGNRSLGVMLPYTPLHHLLLAEVGRPIVLTSGNVSDEP
                     IAYRDDDALERLGGIADWFLVHNRPIHVRVDDSVVRSFRGRELPLRRSRGFAPQPLGL
                     PWPFPRHVLACGAELKHTFCLAKGDRAFVSHHVGDLENYETYRSFTEGVEHYRRLFEV
                     SPEVVAHDLHPEYLSTKHALELDGVELEGVQHHHAHVAACLADNGEPGPVIGVAFDGL
                     GYGTDGTIWGGELLVADLEGFRRAGHLETVPMPGGTAAIKEPWRMAAAWLDAALGAQV
                     PERLEVVGRNLGRWERVVALARSGTASPVTSSAGRLFDAVAAILGVRDAVNYEGQAAV
                     ELEQLADPAEPAAYPARVTGVGPGGWPGGLVLAGTDLVRAVVEDLEAGAAPPLVAARF
                     HNGLAAATIAACQAVRETTGLEAVALSGGVFQNLLLLDGVVAGLEGQGFRVLVHSRVP
                     PNDGGISLGQAAVAGARSRARAT"
     gene            complement(2222..2650)
                     /locus_tag="VFO47_02260"
     CDS             complement(2222..2650)
                     /locus_tag="VFO47_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787556.1"
                     /translation="MDEREEIEQVQPAGEQEPGGLVPPYEGRQTEMKEGYEEHTAKVF
                     HGADDVPPGPGREISDEEREGVPPTDTEATSPLGVGVSMTRRGEDVGRQESEPGREDT
                     GTKGASDRPVGVSDERASTTVDPDETESGGPTLPAGDQGG"
     gene            complement(2663..>2945)
                     /locus_tag="VFO47_02265"
     CDS             complement(2663..>2945)
                     /locus_tag="VFO47_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET8787557.1"
                     /translation="EEAVPVRPDVRGACELLGIDPLYVACEGRLVAVVDGDQVDAAMG
                     ALRSHPLGEGAAVIGRVRDDPPGLVLLKTSFGGTRIVDLLVGDPLPRIC"
BASE COUNT          326 a         1130 c         1111 g          378 t
ORIGIN      
        1 tgcgcgagga cgcgccgccg ctggcgatcg tggagcgcgt gctcacggag cccgtcgagc
       61 cgctgccggg gacgggcttc cggatcgtgg agtccgcgcg cggcgggcgc ccgtcggcgc
      121 tcgtctccgc ggatatcgcc acctgcgacg actgcctgcg cgagctcttc aaccccgccg
      181 accgccgcca ccgctacccg ttcctgaact gcaccaactg cggcccgcgc ttcacgatcg
      241 tgaccggcat cccgtacgac cggcccaaca cgacgatggc cggcttcgtg atgtgcccgg
      301 cctgccggcg cgagtacgac gacccggccg accgccgctt ccacgcccag ccggtgtgct
      361 gcccggcctg cgggccggcg ctggccctgc tggaccgcga ggggcgggcc gcgggcgacg
      421 acccgctcga ggcggccgcg gcccggctcc gggacggggc cgtggtcgcc gtcaagggcc
      481 tcggcggcta ccacctggcc gccgacgcgg ccagcgagcc ggccgtggcc gcgctgcggg
      541 cccgcaagca ccgcgaggac aagccgttcg cggtgatggc ggccgacctg gacggggcca
      601 gggcgctctg cctggtcgac cgggccgagg aggccatgct ggccagcccc cggcgcccga
      661 tcgtcctgct gcggcggcgc ccggacctcc gggccggcgt ggccgggccg gtggcgccgg
      721 ggaaccggtc gctgggggtg atgctcccct acacccccct gcaccacctg ctgctggccg
      781 aagtcgggcg gccgatcgtg ctcaccagcg gcaacgtctc cgacgagccg atcgcctacc
      841 gggacgacga cgccctggag cggctcggcg ggatcgccga ctggttcctg gtccacaacc
      901 ggccgatcca cgtccgggtc gacgactcgg tggtgcggtc gttccggggg cgcgagctgc
      961 ccctgcgccg ctcccgcggg ttcgcgcccc aaccgctggg gctgccctgg ccgttccccc
     1021 gccacgtcct ggcctgcggg gccgagctca agcacacctt ctgcctggcc aagggcgacc
     1081 gcgccttcgt ctcccaccac gtcggcgacc tggagaacta cgagacctac cggtccttca
     1141 ccgagggggt cgagcactac cggcggctgt tcgaggtctc cccggaggtg gtcgcccacg
     1201 acctgcaccc cgagtacctg tcgaccaagc acgcgctcga gctggacggg gtcgagctgg
     1261 agggcgtgca gcaccaccac gcccacgtgg ccgcctgcct ggccgacaac ggcgagccgg
     1321 ggccggtcat cggggtcgcc ttcgacgggc tcggctacgg caccgacggg accatctggg
     1381 gcggggagct gctggtcgcc gacctggagg ggttccggcg ggccggccat ctggagacgg
     1441 taccgatgcc gggcgggacg gccgccatca aggagccgtg gcggatggcc gccgcctggc
     1501 tggacgccgc cctcggcgcc caggtgcccg aacggctgga ggtggtcggg cgcaacctgg
     1561 gccgctggga gcgggtggtc gccctggcca ggtcgggcac ggcctcaccg gtgacctcga
     1621 gcgccgggcg gctgttcgac gcggtggcgg cgatcctggg cgtccgcgac gcggtcaact
     1681 acgagggcca ggcggcggtc gagctggagc agctcgccga cccggccgag ccggccgcct
     1741 acccggcccg ggtcaccggg gtggggccgg ggggctggcc tggcgggctg gtcctggccg
     1801 ggaccgacct ggtccgggcg gtggtcgagg acctggaggc cggggcggcg ccgccgctgg
     1861 tcgccgcccg cttccacaac ggcctggccg cggccaccat cgccgcctgc caggccgtca
     1921 gggagaccac cggcctggag gcggtggccc tctccggcgg cgtcttccag aacctcctgc
     1981 tcctggacgg cgtggtcgcg ggcctggaag gccagggctt ccgggtcctg gtccactccc
     2041 gggtcccccc caacgacggc ggcatcagcc tcggccaggc cgcggtcgcc ggcgcccgtt
     2101 cgcgggcccg ggcgacctag cgcgagcgcc ctgagcacgc ctgggaaggt tcgcctgaca
     2161 gcctgcccgc aggcagagcc cgtccgcccc accggccggg gggccttcag gagcccccga
     2221 actacccgcc ctggtcgccg gcggggaggg tggggccgcc ggactcggtc tcgtcggggt
     2281 cgaccgtggt ggaggcgcgc tcgtcggaga cgccgaccgg gcggtcggag gcgcccttgg
     2341 tgccggtgtc ctcgcggcct ggctcggatt cctgcctgcc gacgtcctcg ccgcggcggg
     2401 tcatgctcac gccgaccccg agcggggagg tggcctcggt gtcggtcggg gggacgccct
     2461 cgcgctcctc gtcggagatc tcacggccgg ggccgggggg gacgtcgtcg gcgccgtgga
     2521 agaccttcgc ggtgtgctcc tcgtagccct ccttcatctc cgtctggcgg ccctcgtacg
     2581 gcggcaccag cccgcccggc tcctgctccc cggccggctg cacctgctcg atctcttcgc
     2641 gctcgtccat gcccggcccc tttcagcaga tccgcggcag ggggtccccg accagcaggt
     2701 cgacgatccg ggtcccgccg aacgacgtct tcaacagcac caggccgggc gggtcgtccc
     2761 tgacccggcc gatgacggcg gcgccctcgc cgagggggtg ggagcgcagg gcgcccatgg
     2821 ccgcgtcgac ctggtcgccg tcgacgaccg cgaccagccg gccctcgcag gcgacgtaca
     2881 gggggtcgat gccgagcagc tcgcaggccc cgcggacgtc cgggcggacc ggcaccgcct
     2941 cctcg
//
LOCUS       DASSWR010000140         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_768902,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000140 DASSWR010000000
VERSION     DASSWR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768902"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFO47_02270"
     CDS             complement(<1..567)
                     /locus_tag="VFO47_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET8787558.1"
                     /translation="MPRGATADGAWSLAVTPEQAGWAYCGLRVLELAPGAAHTFATGA
                     DELLVMPMEGGGVVECESRRFELAGRDGVFGDVTDFAYVPRDAEVRVSSAGGARFILP
                     SARATSRLEPAYGPASGVPVALRGAGQASRQVNNFCTPASFPADKMIVVEVMTPGGNW
                     SSYPPHKHDERRTDEVELEEIYYFEVAGD"
     gene            complement(579..1466)
                     /locus_tag="VFO47_02275"
     CDS             complement(579..1466)
                     /locus_tag="VFO47_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HET8787559.1"
                     /translation="MLDERYRTVLEARTRRPEVIAEAAAARTRPASLFNEYGRLMMIA
                     ADHPARGALRAGERTLAMADRVDLLDRLCLALSRPGVNGVLGTPDILEDLLLLGALES
                     KVVVGSMNRGGLAGTVFEIDDRFTAYDAASLARDGFEGGKMLLRIDPDDPATVATVEA
                     CGRAVSELAAHRLMAMVEPFISHRVDGRVRIDLGVEATVRAMTVAAGIGNTSAYTWLK
                     VPVVEEMERVMAATTLPALLLGGEVSSAQDAAFESWRKALKLPTVQGLVIGRSLLYPP
                     DDDVATAVDTAVGLLQEGQ"
     gene            complement(1456..2412)
                     /gene="iolC"
                     /locus_tag="VFO47_02280"
     CDS             complement(1456..2412)
                     /gene="iolC"
                     /locus_tag="VFO47_02280"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HET8787560.1"
                     /translation="MTAIFDVLTMGRIGVDVYPLQVGVSLREVETFGKYLGGSPSNVA
                     VAAARYGRRSAVITRTGQDPFGEYLHDALRGFGVDDRFVTAVPGLPTPVTFCEIFPPD
                     NFPLYFYRFPKAPDLEIRPEELDLDAIRSARVFWVTVTGLSQEPSRSATLAALEARAR
                     SGITILDLDYRPMFWPSREAARAEVQRALGHVTVAVGNLDECETAVGEREPRAAAGAL
                     RDRGVEVVVVKQGPKGVLARDAGTEVEVPPVPVEVVNGLGAGDAFGGALCHGLLAGWE
                     LERLMRFCNAAGALVATRLACADAMPEAAEVEALLEEAVNAG"
     gene            2598..3341
                     /locus_tag="VFO47_02285"
     CDS             2598..3341
                     /locus_tag="VFO47_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8787561.1"
                     /translation="MSFSPASITVDRYSPVPMYYQVAQQLEHAIETGELAPGTRLDGE
                     LALANQLGVSRPTLRRAIEYLVDRGYLVRRRAVGTQVVHPKVRRPVELSSLYDDLTAS
                     RKNPRTTVLSIDRVPATDAVAHALGLEEGTGVLAVERLRYADGQPLAIMRNWLPLGLV
                     DLDAGRLERAGLYQLMRAAGINLHLASQTIGARGASGAEAKLLQASTGEPLLTMTRTT
                     YNENGQPVELGDHLYRASLYSFEIVLVTR"
BASE COUNT          499 a         1230 c         1212 g          455 t
ORIGIN      
        1 gtcgccggcg acctcgaagt agtagatctc ctccagctcg acctcgtcgg tgcgccgctc
       61 gtcgtgcttg tggggcgggt aggaggacca gttgccgccg ggggtcatga cctcgaccac
      121 gatcatcttg tcggccggga acgacgccgg ggtgcagaag ttgttgacct gccggctggc
      181 ctggccggcc ccgcgcagcg ccaccgggac gccggaggcc ggcccgtagg cgggctcaag
      241 gcgcgaggtc gcccgcgccg agggcaggat gaaccgggct ccgccggcgc tggagacgcg
      301 cacctcggcg tcccggggga cataggcgaa gtcggtcacg tcgccgaaca cgccgtcgcg
      361 gccggccagc tcgaaccggc gcgactcgca ctcgaccacg ccgccgccct ccatgggcat
      421 gaccagcagc tcgtcggccc cggtggcgaa ggtgtgggcg gcgccggggg cgagctccag
      481 gacccgcagc ccgcagtagg cccagccggc ctgctcgggg gtgacggcca gcgaccaggc
      541 gccgtcggcc gtggcgcccc tcggcaggta ccacttgctc actggccctc ctggagcagg
      601 ccgaccgcgg tgtcgacggc cgtggcgacg tcgtcgtcgg gcgggtacag cagcgaccgg
      661 ccgatgacca ggccctgcac ggtggggagc ttgagggcct tgcgccagct ctcgaaggcg
      721 gcgtcctggg cgctgctgac ctcgccgccc agcagcaggg cgggcagggt ggtggcggcc
      781 atgacccgct ccatctcctc caccaccggg accttgagcc aggtgtaggc ggaggtgttg
      841 ccgatgccgg cggccaccgt catggcccgg acggtcgcct cgacgcccag atcgatccgg
      901 acccggccgt cgacccggtg ggagatgaac ggctcgacca tggccatcag ccggtgcgcg
      961 gccagctcgc tgacggcccg gccgcaggcc tcgaccgtgg ccaccgtggc cgggtcgtcg
     1021 gggtcgatcc gcagcagcat cttgccgccc tcgaagccgt ccctggccag gctggccgcg
     1081 tcgtaggcgg tgaagcggtc gtcgatctcg aacacggtcc cggccaggcc gccgcggttc
     1141 atcgagccca cgaccacctt gctctccagg gcccccagca gcagcaggtc ctccaggatg
     1201 tcgggagtgc cgaggacgcc gttgacccct ggccgcgaga gggccaggca gaggcggtcg
     1261 agcaggtcga cccggtcggc catggccagg gtccgctcgc cggcccgcag ggcgccgcgc
     1321 gccgggtggt cggccgcgat catcatcagc cggccgtact cgttgaacag cgacgccggg
     1381 cgggtccggg cggcggcggc ctcggcgatc acctccgggc gccgcgtccg ggcctcgagc
     1441 acggtccggt agcgctcatc cagcattgac ggcctcctcg agcagcgcct ccacctcggc
     1501 ggcctcgggc atggcgtcgg cgcaggccag ccgggtcgcg accagcgccc cggcggcgtt
     1561 gcagaagcgc atcagccgct ccagctccca gccggcgagc agcccgtggc agagcgcccc
     1621 cccgaacgcg tcgccggcgc cgaggccgtt gaccacctcg accggcaccg gcggcacctc
     1681 cacttcggtg ccggcgtccc gggccagcac ccccttgggg ccctgcttca ccaccacgac
     1741 ctcgactccg cggtcgcgca gggccccggc ggcggcacgc ggctcgcgct cgccgacggc
     1801 cgtttcgcac tcgtcgaggt tcccgaccgc gaccgtgacg tgcccaaggg cccgctgcac
     1861 ctcggcgcgg gccgcctccc gggacggcca gaacatcggc cggtagtcga ggtcgaggat
     1921 ggtgatgccc gacctggccc gggcctccag ggcggccaga gtggcgctgc ggctcggctc
     1981 ctgggaaagg ccggtcaccg tcacccagaa cacccgggcc gaccggatcg cgtccaggtc
     2041 cagctcctcg gggcggatct cgaggtcggg ggccttgggg aagcggtaga agtagagcgg
     2101 gaagttgtcg ggcgggaaga tctcgcagaa ggtcaccggc gtcggcaggc cgggcacggc
     2161 ggtcacgaaa cggtcgtcga ccccgaagcc ccgcagggcg tcgtgcaggt actcgccgaa
     2221 ggggtcctgg ccggtgcggg tgatgacggc gctgcgccgc ccgtagcggg ccgcggccac
     2281 ggccacgttg ctcgggctgc cccccaggta cttgccgaag gtctcgacct cgcgcaacga
     2341 caccccgacc tgcaggggat agacgtcgac cccgatccgt cccatggtga ggacatcgaa
     2401 gatcgcggtc atggcgtgcc tcccaccaga gctgctgagg gaccgggatc gccaaagggg
     2461 actagacagc gattgttaga atacggtaac tactactcat tagatactca tgtcaacaga
     2521 tactcatgtc aaccccttgt cctaacttgg tgacaatgtt aggtcaggag aactcgcacc
     2581 actcgggggc tgggcgcatg tcgttctcgc cggcgtcgat caccgtggat cgatacagcc
     2641 cggtgcccat gtactaccag gtcgcccagc agctggagca cgccatcgag acgggcgagc
     2701 tcgctcccgg cacccgcctg gacggtgagc tggccctggc caaccagctc ggggtgtcgc
     2761 ggccgacgct gcggcgggcc atcgagtacc tggtcgaccg cggctacctg gtccgccggc
     2821 gggccgtcgg cacccaggtc gtccacccca aggtgcggcg gccggtcgag ctgtccagcc
     2881 tgtacgacga cctgacggcc agccgcaaga acccccggac cacggtgctg tcgatcgacc
     2941 gggtcccggc gaccgacgcc gtcgcccacg ccctcgggct ggaggagggc accggcgtgc
     3001 tggccgtcga gcggctgcgc tacgccgacg gccagccgct ggccatcatg cgcaactggc
     3061 tccccctggg gctggtcgac ctcgacgccg ggcggctgga gcgggccggg ctgtaccagc
     3121 tcatgcgggc cgccgggatc aaccttcacc tggcctccca gaccatcggg gccagagggg
     3181 ccagcggcgc cgaggccaag ctgctccagg cctcaacggg tgagccgctg ctgaccatga
     3241 cgcgcaccac ctacaacgaa aatggtcagc cggtcgagct tggcgaccac ctctaccggg
     3301 caagcttgta ctcgttcgag atcgttctcg tcacgcggta agtccccggc aggagggagg
     3361 gccctcacat ggccagtatg gaaggtcagg tcgcgc
//
LOCUS       DASSWR010000141         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_485665,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000141 DASSWR010000000
VERSION     DASSWR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485665"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="ppk2"
                     /locus_tag="VFO47_02290"
     CDS             complement(<1..779)
                     /gene="ppk2"
                     /locus_tag="VFO47_02290"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET8787562.1"
                     /translation="MAKRNKRQHEHADPPPKLKRKDYEREMRRLHGELVAMQEWVKAS
                     GAKICIVFEGRDTAGKGGTIKRITERVSPRVFRVVALPTPTEREKTQMYVQRYLPHLP
                     AAGEVVIFDRSWYNRAGVERVMGFCKPEETERFLELTPSVEKTMVDSGILLLKYWLEV
                     GPDEQTRRLESRIEDPRKVWKLSDMDLKSYSRWYDYSRARDAMFAATDTAWAPWYIAR
                     TDDKQRGRLNLIRHLLSQVPYKPLVQKDVTLPKRQRAGGYV"
     gene            complement(879..2237)
                     /locus_tag="VFO47_02295"
     CDS             complement(879..2237)
                     /locus_tag="VFO47_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET8787563.1"
                     /translation="MVATEQETPGTATVPQRWLARLALVAAAAAVLVPPVLAGLRQSL
                     ALVLVGLVGLALALAGAWWALTNRGLVRGLAIALAVAAPLTVLVLYRRERLLWVVLVA
                     LGLLALAVAAGRAALRGDPTPEKMPEYDVPPPRRPFMIMNPRSGGGKVVRFGLKDKAE
                     ALGATVALLEGPGVVDVGELARRAVADGADLLGVAGGDGTQALVAGIAAEHGIPLMVI
                     SAGTRNHFALDLGLDREDPSRCLDALRDGVELHVDLGFIGDRPFVNNASFGAYAAVVQ
                     SPAYRDDKTRTTLDLLPGLLVGHQGPRLVARAGNVTIEGPQAVLVSNNVYGMGDIAGL
                     GRRARLDQATLGLFAVTVASAVQAAGLVRGTQSRGLTRLAGRQVVVDADASQIPVGVD
                     GEALVLDTPVRCTVQPAALRVRVPRDRPGVPDPKPIMDWKRLWHIALTVSGPVSGAAR
                     PP"
BASE COUNT          358 a          890 c          829 g          355 t
ORIGIN      
        1 tccacatagc cgccggcccg ctgccgcttg ggcagcgtga cgtccttctg gaccagtggc
       61 ttgtagggga cctggctgag caggtggcgg atgaggttga gccggcctcg ctgcttgtca
      121 tcggtgcggg cgatgtacca gggcgcccag gcggtatcgg tggcagcgaa catggcgtcc
      181 ctggccctgg agtagtcgta ccagcggctg taggacttca ggtccatgtc cgacagcttc
      241 cagaccttgc gggggtcctc gatgcggctc tccagccgcc gggtctgctc gtcggggccg
      301 acctccagcc agtacttcag gagcaggatc cccgagtcga ccatggtctt ctcgaccgat
      361 ggcgtcagct cgaggaaccg ctccgtctcc tccggcttgc agaagcccat cacccgctcg
      421 acgccggcgc ggttgtacca gctgcggtcg aaaatcacga cctcgccggc ggccggaagg
      481 tgcgggaggt accgctggac gtacatctgg gtcttctcgc gctcggtcgg ggtgggcagg
      541 gcaacgaccc ggaacacccg cgggctcacc cgctcggtga tccgtttgat cgtgcccccc
      601 ttcccggcgg tgtcccggcc ctcgaacacg atgcagatct tggcccccga cgccttgacc
      661 cactcctgca tggccacgag ctcgccgtgc aggcgccgca tctcccgctc gtagtccttg
      721 cgcttcagct tcggaggcgg gtcggcgtgc tcgtgttgcc gcttgttcct cttggccata
      781 tcgtttcacc tccaaggcgc tcgcttcccc aaagatccgg gttgagcatg ccctgcccga
      841 tccttcctgc aggcgtacgg cttctgttgg agatctcctc aaggtggccg ggccgccccg
      901 gagaccgggc cagatacggt cagggcgatg tgccacagcc gcttccagtc catgattggc
      961 ttgggatcgg gcacaccagg acggtcacgg ggcacccgga cccgcagggc ggccggctgc
     1021 acagtgcagc ggacgggcgt gtccagcacc agcgcctcgc cgtcgacccc aaccgggatc
     1081 tgggatgcat cggcgtcgac cacgacctgg cgcccggcca gcctggtgag cccacgggat
     1141 tgtgtcccgc ggacaaggcc ggccgcctgg accgcgctgg cgacggtgac ggcgaacagg
     1201 cccagggtcg cctggtcgag ccgggcccgg cgccccagcc cggcgatgtc gcccatcccg
     1261 tagacgttgt tggagaccag gaccgcctgc ggcccctcga tggtcacatt cccggcccgg
     1321 gcgaccagcc gcggcccctg gtggccgacc agcagtcctg gcagcaggtc cagggtggtg
     1381 cgggtcttgt cgtcgcggta ggccgggctc tgcaccacgg cggcataggc gccgaaggag
     1441 gcgttgttga cgaacgggcg gtcgccgatg aaccccaggt cgacatggag ctcgacaccg
     1501 tcgcgcagcg catccaggca gcgggatggg tcttcacggt ccagccccag atccagggcg
     1561 aagtggttgc gggtgccggc cgagatgacc atcagcggga tgccgtgctc ggccgcgatg
     1621 ccggcgacca gcgcctgggt cccatccccg ccggccaccc ccagcaggtc ggccccgtcg
     1681 gccaccgccc ggcgggccag ctcacccaca tccaccaccc cgggcccttc cagcagggcg
     1741 acggtggcgc cgagggcctc ggccttgtcc ttgaggccga acctgaccac cttcccgccg
     1801 ccggagcgcg ggttcatgat catgaacggc cgccggggcg gcgggacgtc gtactcgggc
     1861 atcttctcgg gggtggggtc gccgcgcagc gcggcccggc cggcggcgac ggccagggcc
     1921 agcagcccca aggccaccag cacgacccag agcagccgct cgcgccggta caggaccagg
     1981 acggtcagcg gggccgccac cgccagggca atcgccaggc cccgcaccag gcccctgttg
     2041 gtcagcgccc accacgcccc ggccagggcc agggccaggc cgaccagccc gaccagcacc
     2101 aaggccaggc tctggcggag accggccagc accggcggca ccagcacggc cgcggcggcg
     2161 gccaccaggg ccagacgggc cagccagcgc tgggggacgg tcgccgtccc tggcgtctcc
     2221 tgctcagtgg ccaccatgcg actgctcacc gcccacttcc ctcagctgcc ggcaccacct
     2281 gcaagcgtgg acgagggacg cccagcgatg catcacccca cacggatgag atccaggacc
     2341 catcccggca cctcatgagt cggtacctcc ccggcatagc ggtggaggta gcccgtcagg
     2401 gacgtcccgt cgggatctgg gccatatgtg ag
//
LOCUS       DASSWR010000142         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_146982,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000142 DASSWR010000000
VERSION     DASSWR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146982"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(518..778)
                     /locus_tag="VFO47_02300"
     CDS             complement(518..778)
                     /locus_tag="VFO47_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET8787564.1"
                     /translation="MQKQQLQTRLRRIEGQVRGVARMIDEDKYCVDVVTQVAAIQAAL
                     DKVSLGLLDGHIRGCVREEIQAGGGDAKVDELLQVMDRVLRR"
     gene            885..>2638
                     /locus_tag="VFO47_02305"
     CDS             885..>2638
                     /locus_tag="VFO47_02305"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET8787565.1"
                     /translation="MAAAAPERAGATRVDLPIEGMTCGACAARIERGLLRIDGVASAS
                     VNLAAERATVVYDPAVTGPPAFAEKVASLGYAVGTEPDGDEGGDEGRTRLLAAAALTV
                     PVLALSMFPALRYPGWAWLAAVLATPVVLWAGAGFHRAALAGLRHRTATMDTLVSLGT
                     LAAWGWSVAVLLAGDPAAADPGGHVYFEVASVIVVLILLGRWLEGRARRRSGQALRRL
                     LELGAGTAMVLRDGAEVPVPAEAVAVGDRFVVRPGQKLATDGVVEQGRSSIDRSLLTG
                     ESVPVEVGPGDDVTGATVNLEGRLVVRATRVGADTALAQIVRLVEAAQGSKAPVQRLA
                     DRVAGVFVPVVLGVAAVTLAGWLVTGHDAGEAVSAAVAVLIVACPCALGLATPTAVMA
                     GTGRGAQLGILIRGAEVLERAGRVTTALLDKTGTVTEGRMSLVEVAADDGTDPDRLLA
                     LAASLEDASEHPVARAVADGARDRGLALSPAEAFRNLPGRGVSGRVGGHELLVGSAGL
                     LEERGWTVPERVVAAADAMRAAGRTAVVAGWDGRARACWPSPTSSSRAHGRRWTGSGP
                     STSRPSCSQATIRPQLAP"
BASE COUNT          283 a          994 c         1040 g          321 t
ORIGIN      
        1 gaggacgggc acggccacca cctgctcacg gctgggccgg cgcttgtccg agcgggcgta
       61 ggcggccttg tggcggcact ggtcgcgctt gatcagggtc cacggctccg gcttcccgtc
      121 ggcccgccag ggcaacgtgg ccgagcgcgg ggccggcgtc gacgaaggcg ccgccgaagc
      181 tctggcagcg gacggccacc ctggccccgg ccagcaccga ggcggccccg ccgaggcggc
      241 gttcctggtg ggcccggagc accccccggg acggccgcgg ccacggccat gaccagcagc
      301 agcgagacgc tgacccaggg gaaccggcgc ggccgaccca gctcgtccaa gcggcgcagg
      361 ctccagccgg cggcgaccgc gaccgcgccc ccggccaggg cgaccgcgaa ccaggtcagc
      421 accgcagctc tccgtcgccg cgcaacggtc cgttcaggga gcatcggcag atccggcgca
      481 gcgcgtgagc gcgcgggtgc gccggcggcc ggggagctca gcggcgcagg acccggtcca
      541 tcacctggag cagctcgtcg accttggcgt cgccgccgcc ggcctggatc tcctcgcgga
      601 cgcagccgcg gatgtggccg tcgagcagcc ccagcgacac cttgtcgagg gcggcctgga
      661 tggccgcgac ctgggtgacc acgtcgacgc agtacttgtc ctcgtcgatc atgcgggcca
      721 cgccgcggac ctggccctcg atgcggcgca ggcgcgtctg gagctgctgc ttctgcatgg
      781 tatagccggg cacggcgcct cctctcttgc acgccccgat accccggtag cgtatcctaa
      841 gatacccggg gtgggtatca ggagcgacgc cgacggaggt ggcggtggcg gcagcggcgc
      901 cggagcgggc gggcgcgacc agggtcgacc tgccgatcga gggcatgacc tgcggcgcct
      961 gcgcggcccg gatcgagcgg ggcctgctcc ggatcgacgg ggtcgcctcg gccagcgtca
     1021 acctggccgc cgagcgggcc actgtcgtct acgacccggc ggtcaccggc cccccggcgt
     1081 tcgccgagaa ggtggcctcc ctcggctatg ccgttggcac cgagccggat ggcgacgagg
     1141 gtggggacga gggccgcacc cgcctgctgg ccgcggccgc gctcaccgtt cccgtcctcg
     1201 ccctgtccat gttcccggcc ctgcggtacc ccggctgggc gtggctggcc gcggtcctgg
     1261 ccaccccggt ggtgctgtgg gccggggccg gcttccaccg ggccgccctg gccgggctgc
     1321 ggcaccggac cgcgaccatg gacacgctgg tctcgctggg caccctggcc gcctggggct
     1381 ggtcggtggc ggtgctgctg gccggcgacc cggccgccgc ggaccccggc ggccacgtct
     1441 acttcgaggt cgccagcgtg atcgtggtcc tgatcctgct cggccgctgg ctggaggggc
     1501 gggcccggcg ccgctccggg caggccctgc gccgcctgct ggagctgggc gcgggcacgg
     1561 ccatggtgct gcgcgacggg gccgaggtcc cggtcccggc cgaggcggtg gcggtcggcg
     1621 accggttcgt ggtccggccc ggccagaagc tggccaccga cggggtggtg gagcagggcc
     1681 gctcctcgat cgaccggtcg ctgctgaccg gcgagtcggt cccggtcgag gtcggcccgg
     1741 gcgacgacgt caccggggcc accgtcaacc tggagggccg gctggtggtg cgggcgaccc
     1801 gggtcggggc cgacaccgcc ctggcccaga tcgtgcggct ggtcgaggcc gcccagggct
     1861 ccaaggcccc ggtgcagcgc ctggccgacc gggtcgcggg cgtgttcgtg ccggtggtcc
     1921 tgggcgtcgc cgccgtcacc ctggccggct ggctggtcac cggccacgac gccggcgagg
     1981 cggtctcggc cgccgtggcc gtgctcatcg tggcctgccc gtgcgcgctc ggcctggcca
     2041 ccccgaccgc ggtcatggcc gggaccggcc ggggcgccca gctcggcatc ctcatccgcg
     2101 gggccgaggt gctggagcgg gccggccggg tgacgaccgc cctgctcgac aagacgggca
     2161 cggtcaccga gggccgcatg tccctggtcg aggtggccgc cgacgacggc accgacccgg
     2221 accggctgct ggccctggcg gccagcctcg aggacgcctc cgagcacccg gtggcccggg
     2281 ccgtggccga cggcgcccgg gaccgcgggc tggccctctc ccccgccgag gcgttccgga
     2341 acctccccgg ccggggcgtc agcggccggg tcggcggcca cgagctgctg gtcggctcgg
     2401 ccgggctgct ggaggagcgg ggctggaccg tccccgagcg ggtcgtggcc gccgccgacg
     2461 ccatgcgggc ggccggccgg accgcggtcg tggccggctg ggacggccgg gccagggcat
     2521 gctggccgtc gccgaccagc tcaagccggg cgcacgggcg gcggtggacc ggctcagggc
     2581 cctcgacctc gagaccgtcc tgctcacagg cgaccatcag gccacagctg gctccgtg
//
LOCUS       DASSWR010000143         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_401127,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000143 DASSWR010000000
VERSION     DASSWR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401127"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFO47_02310"
     CDS             complement(<1..416)
                     /locus_tag="VFO47_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787566.1"
                     /translation="MKLGPNFHKALAASGSSNLSDGVFQVGLPLLAVQLTRSPLLIAG
                     VSVAARLPWLLALVAGALADRLDRRQTMVRVDLFRAVLLGGLALAVAADLATLAMLYV
                     VALLLGLAETLFDTSAQSLLPAIVSRDDLTVANSRL"
     gene            471..1085
                     /locus_tag="VFO47_02315"
     CDS             471..1085
                     /locus_tag="VFO47_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET8787567.1"
                     /translation="MEPTIRGPVVRRPATAAEAKALAHPLRMRILRLCLDRSLTNKQL
                     AEWLDKDPGTVLHHVRTLVNTGFLVPDEVRQGEKGALEKPYRATGKSWTLSLDESPVP
                     QDADAQAMLEAFQAELAEAGPGASAGFNRLALTLSRASREELESRVLAILDEYVTRPP
                     DPDGEPYGLLFAMHRRSLGPDPSGGPARRPRGHASGSGGVAPRS"
     gene            complement(1046..1591)
                     /locus_tag="VFO47_02320"
     CDS             complement(1046..1591)
                     /locus_tag="VFO47_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET8787568.1"
                     /translation="MTRLLTLGHGTASATELLALLDGAGVARLVDVRTAPGSRRHPHV
                     RREAMAGWLGAAGVGYRWEPELGGFRRPGPESVNHALRHPAFRGYADHMRTPPFWAAL
                     DRLLAEAAADPTAAMCSETLWWRCHRRLLADAAALVRGVEVLHLDHRGRLEPHRPTEG
                     VRRDGDLLAYDLGATPPLPEA"
     gene            complement(1588..2514)
                     /locus_tag="VFO47_02325"
     CDS             complement(1588..2514)
                     /locus_tag="VFO47_02325"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET8787569.1"
                     /translation="MPRNPFANPLFLPVFLRVMLLIVVGLAAVLLSQRRSLKVWRRST
                     LFLRARTWFVIGPVFVLAVFIGGFVGFLLPTFVVVQGCSEFARIAGIERRYAYLLILW
                     GELGLLVAALARDFFGFLPFGFFIALTLLPILSGQITDAHRQVADTLVGYVFVALPMA
                     YIVLVKSAEPNGLEFLLIVTVAVALSDIAAYATGSALRGRKLMPRVDPTKTWGGVLGN
                     LVGAAVGVGWLWVAVPPEWSIAAVVVLVLVITFGSVWADLTGSFIQRAFRTEVRSTVI
                     LGYGGVLSRIDSLLITFPLSYYALILVDKLVG"
     gene            complement(2523..>2784)
                     /locus_tag="VFO47_02330"
     CDS             complement(2523..>2784)
                     /locus_tag="VFO47_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787570.1"
                     /translation="TRGTGASDFKKGFAYLAIDTGVPVVPMYLHGLEQVMPKGSFVPL
                     PGGVVVGIGLPIPPGDDYNDLVRRAEAGVAEVRAVVKGWEGA"
BASE COUNT          462 a          991 c         1032 g          299 t
ORIGIN      
        1 aacagccggc tgttggccac ggtgaggtcg tcgcgggaca cgatggcggg cagcagcgac
       61 tgggccgagg tgtcgaacag ggtctcggcc agcccgagca gcagggcgac gacgtacagc
      121 atggccaggg tggccaggtc ggccgccacg gccagggcca gccccccgag caggacggcc
      181 ctgaacaggt cgacccggac catcgtctgg cgccggtcca gccggtcggc cagggccccg
      241 gccaccaggg ccagcagcca cgggagccgg gcggcgacgc tgaccccggc gatcagcagc
      301 ggcgaccggg tgagctggac ggccagcagg ggcaggccga cctggaagac cccgtcggac
      361 aggttggagg acccggaggc ggccagcgcc ttgtggaagt tcggtcccag cttcatgggt
      421 ggagaccata gttcattgat ttggaaaagt caattgatag aatcttcccc atggaaccga
      481 cgatcagggg gccggtggtg cgccggccgg ccacggcggc cgaggccaag gccctggccc
      541 acccgctgcg catgcgcatc ctccggctct gcctcgaccg gtcgctgacc aacaagcagc
      601 tggccgagtg gctggacaag gacccgggca cggtcctcca ccacgtccgc acgctggtga
      661 acaccggctt cctggtcccc gacgaggtcc gccagggcga gaagggcgca ctcgagaagc
      721 cctaccgcgc caccggcaag tcctggaccc tgagcctcga tgagagcccg gtcccccagg
      781 acgccgacgc ccaggcgatg ctggaggcgt tccaggccga gctggccgag gccgggcccg
      841 gcgccagcgc cggcttcaac cgcctcgccc tcaccctcag cagggcgtcg cgcgaggagc
      901 tcgagtccag ggtgctggcg atcctggacg agtacgtgac ccggcctccc gatccggacg
      961 gcgaacccta cggcctgctg ttcgccatgc accgccggtc gctgggaccc gatccatcgg
     1021 gggggccggc gcggcgcccc cggggtcacg cctcgggcag cggcggggtg gcgcccaggt
     1081 cgtaggcgag caggtcgccg tcgcggcgga cgccctcggt ggggcggtgc ggctccagcc
     1141 ggccccggtg gtccaggtgc aggacctcga ccccgcggac cagcgcggcg gcgtcggcca
     1201 gcagccgccg gtggcagcgc caccacagcg tctccgagca catggccgcc gtcgggtcgg
     1261 ccgcggcctc ggccagcagg cggtcgaggg ccgcccagaa cggcggcgtc cgcatgtggt
     1321 cggcgtaacc gcggaaggcg ggatggcgca gggcgtggtt caccgactcc ggtccgggcc
     1381 tgcggaaccc gcccagctcc ggctcccagc ggtagccgac cccggcggcg cccagccagc
     1441 cggccatggc ctcgcggcgg acgtgcgggt ggcggcggct gcccggggcg gtgcggacgt
     1501 cgaccagccg cgccaccccg gccccgtcca gcagcgccag cagctcggtg gccgacgccg
     1561 tcccgtgccc gagggtgagc agccgggtca tccgaccagc ttgtccacca ggatcagggc
     1621 gtagtaggag agcgggaagg tgatcagcag cgagtcgatc cggctcagga ccccgccgta
     1681 gccgaggatg acggtgctgc ggacctcggt ccggaaggcc cgctggatga agctgccggt
     1741 gaggtcggcc cagacgctgc cgaacgtgat caccaggacc agcacgacca cggcggcgat
     1801 cgaccactcc ggcgggacgg ccacccacag ccagccgacc ccgaccgccg ccccgaccag
     1861 gttgcccagc accccgcccc aggtcttggt cgggtcgacc cggggcatca gcttgcggcc
     1921 ccgcagcgcc gacccggtag cgtaggcggc gatgtccgac agggccacgg ccacggtcac
     1981 gatcagcagg aactcgagcc cgttgggctc ggccgacttg accagcacga tgtaggccat
     2041 gggcagggcc acgaacacgt acccgaccag ggtgtcggcg acctgccggt gggcgtcggt
     2101 gatctggccg gacaggatcg gcagcagggt cagggcgatg aagaagccga acggcaggaa
     2161 gccgaagaag tccctggcca gggcggccac cagcagcccg agctcgcccc agaggatgag
     2221 caggtaggcg tagcggcgct cgatgccggc gatgcgggcg aactccgagc agccctgcac
     2281 gaccacgaag gtgggcagca ggaagcccac gaacccgccg atgaacacgg ccagcacgaa
     2341 caccgggccg atcacgaacc aggtgcgggc gcgcaggaac agggtcgagc gccgccacac
     2401 cttcagcgag cgccgctggg acagcaggac cgcggccaga ccgaccacga tcagcagcat
     2461 cacccgcagg aagacgggga ggaacagggg gttggcgaag gggttgcggg gcacggggcg
     2521 gcctacgccc cttcccagcc cttgaccacc gcgcgcacct cggccacccc ggcctcggcc
     2581 cgccgcacga ggtcgttgta gtcgtcgccg ggcgggatcg ggaggccgat gccgaccacc
     2641 acgccaccgg gcagaggcac gaacgagccc ttgggcatga cctgctcgag cccgtgcagg
     2701 tacatgggga cgaccgggac gccggtgtcg atggccaggt aggcgaagcc cttcttgaag
     2761 tcggaggcgc cggtgccgcg ggtc
//
LOCUS       DASSWR010000144         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_797096,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000144 DASSWR010000000
VERSION     DASSWR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797096"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFO47_02335"
     CDS             <1..309
                     /locus_tag="VFO47_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787571.1"
                     /translation="VAWSMLGVAAVALFASTVTDSPLAAALGALAALVTSQVLDLLDA
                     AAAIKPYLPTHYWLAFVDLFRSPVLWRDVNRGLAVQALYIAVFLGAAWANFATKDIKS
                     "
     gene            337..1089
                     /locus_tag="VFO47_02340"
     CDS             337..1089
                     /locus_tag="VFO47_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET8787572.1"
                     /translation="MRTPASPSMRSHNPALRDDVFTATRLDGGGTMTVQGTVNKTALL
                     LVLAVATATASWVLGTSGGSGVAGWVLVASLAGLGVAIATIVRPRWSPVTAPIYALVE
                     GVVLGVVSMWFEAEFPGIAIQAVALTFGVMGAMLVLYTTRVIKVTQRFRTGVIAATLA
                     IAGVYLVAFLLGLFGVRVPFLYDASPLGILISLAIVAVAALNLVLDFDLIERGARSGA
                     PAYMEWYAAFGLLVTLVWLYLELLRLLGRLRR"
     gene            complement(1108..2319)
                     /locus_tag="VFO47_02345"
     CDS             complement(1108..2319)
                     /locus_tag="VFO47_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8787573.1"
                     /translation="MTRTLLTGGRVFDGTGAPPREADVLVEGARILEVGPGLDGDDAV
                     DVGGRHLLPGLFDCHTHVTLTSIDLMGILQRPFSYRFFETVGNLAATLKAGITTVRDA
                     AGADAGVKRAVADKLVPGPRLQISVTLLSQTGGHGDSWYPSGQHLGLWPHYPGMPENL
                     VDGPEAMRRKVRELVRAGADVIKVATSGGVLSPTDDPRHAHFREDELTALVTEAAAAG
                     RWVMAHAQGSDGIKAALRAGVRSIEHGIYLDDEAIELLLARDAWLVPTLVAPRGVLAA
                     AEAGVPIPDSARRKAAEVVEAHADSFRRAVAAGVKVALGTDSGVTPHGRNLAELELMA
                     KGGMSPAAALAAATSSAAELLGVAGELGTLEPGKRADVVVVDGDPFELATIGDRIAAV
                     VKDGRLVAGRV"
     gene            complement(2316..2735)
                     /locus_tag="VFO47_02350"
     CDS             complement(2316..2735)
                     /locus_tag="VFO47_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787574.1"
                     /translation="MSEITVLGLDNVLLDVGDLEVAKGFYAGRLGLGVKFDFPDAGVV
                     GFRLGDEEAGLVVRAGDVPPGPPRSSPRLWLEVPDARAAGKALAAAGVTLLGEPRELR
                     TGWLVEVADPWGNVVGLTDYLLAPERARQREDGFPRP"
     gene            2863..>3242
                     /locus_tag="VFO47_02355"
     CDS             2863..>3242
                     /locus_tag="VFO47_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787575.1"
                     /translation="MPPDPYERLQAARTAVKALKDERSTLMVRLTELAHGSDEEALVE
                     ASMRLDALPVELLRAELKLARADRQAARATLQAAEASLARALANVRDVKPLPDATVKE
                     KLERAATDGAEAPEAAEGDQDEDSP"
BASE COUNT          405 a         1226 c         1180 g          431 t
ORIGIN      
        1 gtcgcctggt cgatgctggg ggtggcggcg gtggccctgt tcgcctccac cgtcaccgac
       61 tcgcccctgg ccgccgccct cggcgccctg gccgcgctgg tcacctccca ggtcctcgac
      121 ctgctcgacg cggccgcggc gatcaagccc tacctgccca cccactactg gctcgccttc
      181 gtcgacctgt tccgcagccc ggtgctgtgg cgcgacgtca accgtgggct cgccgtccag
      241 gcgctctaca tcgccgtgtt cctcggcgcc gcctgggcca acttcgccac caaggacatc
      301 aagagctagc cttccttgac gaacaggagg acgaccatgc gaaccccggc cagcccgtcc
      361 atgcggagcc acaacccggc cctccgggac gacgtcttca ccgccacccg cctcgacggg
      421 ggcggcacca tgaccgtcca ggggacggtc aacaagaccg ccctgctgct ggtgctggcc
      481 gtggccacgg ccaccgccag ctgggtgctg ggcacgagcg gcgggtcggg ggtggccggg
      541 tgggtcctgg tggccagcct ggccgggctc ggggtggcca tcgccaccat cgtccggccc
      601 cgctggtcgc cggtcaccgc gccgatctac gccctggtcg agggagtggt cctcggggtg
      661 gtgtcgatgt ggttcgaggc cgagttcccc ggcatcgcca tccaggcggt ggccctcacc
      721 ttcggggtca tgggggccat gctggtcctc tacacgaccc gggtgatcaa ggtcacccag
      781 cggttccgga ccggtgtcat cgccgccacc ctggccatcg ccggcgtcta cctggtggcg
      841 ttcctgctcg gcctgttcgg ggtccgggtg ccgttcctct acgacgccag ccccctgggg
      901 atcctcatca gcctggccat cgtggccgtg gccgccctca acctggtgct cgacttcgac
      961 ctgatcgagc gcggggcccg ctcgggcgcc cccgcctaca tggagtggta cgccgccttc
     1021 gggctgctgg tcaccctggt ctggctgtac ctggagctgc tccgcctgct cggccggctg
     1081 cgccgctgag cgccggcgcc gggccgctca cacgcggccg gcgaccaggc ggccgtcctt
     1141 gaccacggcg gcgatccggt cgccgatggt ggccagctcg aacgggtcgc cgtcgaccac
     1201 gaccacgtcg gcccgcttgc ccggctccag ggtgccgagc tcgccggcca cccccagcag
     1261 ctcggccgcc gaggaggtgg cggcggccag cgccgccgcc ggggacatgc cgcccttggc
     1321 catcagctcc agctcggcca ggttgcggcc gtgcggggtg acgccgctgt cggtgccaag
     1381 ggccaccttg accccggcgg cgacggcccg gcggaaggag tcggcgtggg cctcgacgac
     1441 ctcggccgcc ttgcgccggg ccgagtccgg gatgggcacg ccggcctcgg ccgcggccag
     1501 cacccccctg ggcgcgacca gggtcgggac cagccaggcg tcccgcgcca gcagcagctc
     1561 gatcgcctcg tcgtccaggt agatgccgtg ctcgatggag cggaccccgg cccgcagggc
     1621 cgccttgatg ccgtcgctgc cctgggcgtg ggccatcacc cagcggccgg cggcggccgc
     1681 ctcggtgacc agggcggtca gctcgtcctc gcggaagtgg gcgtggcggg ggtcgtcggt
     1741 gggggacagg acgccgccgc tggtcgccac cttgatcacg tcggcgccgg cccggaccag
     1801 ctcgcggacc ttgcgccgca tggcctcggg cccgtcgacc aggttctcgg gcatccccgg
     1861 gtagtgcggc cacagtccca ggtgctggcc cgacgggtac cagctgtcgc cgtggccgcc
     1921 ggtctggctg agcagggtca ccgagatctg caggcggggg ccggggacga gcttgtcggc
     1981 gaccgcccgc ttgaccccgg cgtcggcccc ggcggcgtcg cggaccgtgg tgatcccggc
     2041 cttcagggtc gccgccaaat tccctacggt ctcgaagaag cggtaggaga acggccgctg
     2101 gaggatgccc atcaggtcga tgctggtcag ggtgacatgg gtgtggcagt cgaacagccc
     2161 cggcaggagg tggcggccgc cgacgtcgac cgcgtcgtcg ccgtccagcc cgggcccgac
     2221 ctccaggatg cgggcgccct cgaccagcac gtcggcctcg cggggcgggg cgccggtgcc
     2281 gtcgaacacc cgcccgccgg tcagcagcgt cctggtcacg gacgcgggaa gccgtcctcg
     2341 cgctggcggg cccgctcggg ggccaggagg tagtcggtga ggccgaccac gttgccccag
     2401 gggtcggcga cctcgaccag ccagccggtg cggagctcgc ggggctcacc gagcaaggtc
     2461 accccggccg cggcaagggc cttgccggcc gcccgggcgt ccggcacctc cagccacagc
     2521 cgcggcgacg accggggcgg ccccggaggc acgtcgccgg cccggacgac caggccggcc
     2581 tcctcgtcgc ccagccggaa gccgaccacc ccggcgtcgg ggaagtcgaa cttcaccccg
     2641 aggccgagcc ggccggcgta gaagcccttg gcgacctcca ggtcgccgac gtcgagcagg
     2701 acgttgtcca gtccgagcac ggtgatctcg ctcatggcac gccatgaaag cggcgccggg
     2761 gtggcccgtc aaggcggccc cagcttgaca attggccccg ggagcaggca tcaaggtggc
     2821 cgcgggcggt cttccccctg ccgcccaggg aggtcggtac cggtgccacc tgacccctac
     2881 gagcgtctcc aggcggccag gacggccgtc aaggcgttga aggacgagcg gtccacgctg
     2941 atggtccgcc tgaccgagct cgcccacggc agcgacgagg aggccctggt cgaggcctcg
     3001 atgcggctgg acgccctgcc cgtggagctg ctccgggccg agctcaagct ggcccgggcc
     3061 gaccgccagg cggccagggc caccctccag gcggccgagg ccagcctggc cagggccctg
     3121 gccaacgtcc gcgacgtcaa gccgctgccc gacgccaccg tcaaggagaa gctggagcgg
     3181 gcggccaccg acggtgccga ggcgccggag gcggccgagg gcgaccagga cgaggacagc
     3241 cc
//
LOCUS       DASSWR010000145         7457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_147092,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000145 DASSWR010000000
VERSION     DASSWR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147092"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFO47_02360"
     CDS             complement(<1..283)
                     /locus_tag="VFO47_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787576.1"
                     /translation="MAQQHPTAVPDGLALEREPQPGAGVDGENGATAGAVTRPYDAGL
                     PPEPDVEPLLKRVRQRNPRADTATVGRAFELARGRHGGKRRASGVPYLAH"
     gene            complement(350..895)
                     /locus_tag="VFO47_02365"
     CDS             complement(350..895)
                     /locus_tag="VFO47_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET8787577.1"
                     /translation="MSSSASSGTELPIHVVVEIPKGSRNKYELDHETGRIRLDRMLFS
                     PVHYPTDYGFVPDTLAEDGDPLDALVLLGEPTFPGCEIKGRIVGMLDMADDKGPDQKL
                     LVVPLTDPHWNYIHRIDQVPIHLLREIEHFFSVYKTLEDKTVETFGWRDAYVGHVVLD
                     EAMARGAEHRQAGHGGSTVGA"
     gene            complement(867..1388)
                     /locus_tag="VFO47_02370"
     CDS             complement(867..1388)
                     /locus_tag="VFO47_02370"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET8787578.1"
                     /translation="MDIQRLRALIRDVPDFPKPGIVFKDITPLLANEVAFSSVIDETV
                     VHFGRGNVDKVVGIEARGFILASPVAYHFGAGFLPVRKAGKLPYQVEAEEYALEYGTA
                     TLELHADAVTPGERVLIVDDVLATGGTARAAARLVERLGGKVIGIATLIELEFLEGRR
                     LIEGYEFFSLIRY"
     gene            complement(1416..2669)
                     /gene="secF"
                     /locus_tag="VFO47_02375"
     CDS             complement(1416..2669)
                     /gene="secF"
                     /locus_tag="VFO47_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET8787579.1"
                     /translation="MSLASRLYHGETSYDIVGRRRLWFSISAVLLLISIVSLIWPGLN
                     FGIDFKGGAVFRVQPQEAVTEAQVRDKLGPVAEVVQISQSQPVEVIVQTEVLEPAEVA
                     RIRTELAELGGVERNQVSTQTIGSKWGGTVSRKAIVALLVFLVVVTLYVSLRMEFKMA
                     VAALVALLHDLIITAGIFALARFEVTPATVIALLTILGYSLYDTVVVFDKVRENTGAL
                     TSMSRTTYSQATNLAVNQTMMRSLNTSLASLLPILGLLLVGNYLLGAETLKDLALALF
                     VGVAAGAYSSIFIAPPLVAMWKEKEPRYAQLRARVERLGAQGATERPERPERPARPVR
                     AGGAARDARSRRAAAPPPEPELAPDPELPPDEELEEVAEAEPAEPAVVAQTPARRQPQ
                     KPRPTQQRRKPSGRQQSRPAKRRRR"
     gene            complement(2692..4149)
                     /gene="secD"
                     /locus_tag="VFO47_02380"
     CDS             complement(2692..4149)
                     /gene="secD"
                     /locus_tag="VFO47_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET8787580.1"
                     /translation="MPGTRRYVIPLLVVFALVGVVWAVVLVQGLEPRLGLDLRGGSTV
                     TFVPTNPRGGEPTQQQLDTTVDIIRSRVNSKGVAESEVNLEGGNIVVSIPDVPNPDEV
                     IAAVGTTAQLQFRPVKEVVAPNDPKYNQGEFKAVDCAKPETYSTKDVPDKDVVLCSRT
                     EGRLAPDANSAKLLMGPVALGGTDVASAQAQLAQTGQGGVTTGEWVTALNFSGRGGDK
                     FEELTGKAACNQVGDVKRQIAITLDAVVINSPPVAESIQCDQGISGGQAEITGQTQAE
                     AENLAPLISSGALPLKLDPQTRTTVSATLGADSLRAGLIGGAVGLGLVLLYVLVYYRG
                     LGLVIWVGLVIAAALNVGIVIVMGELIGFTLTLAGIAGLIVAVGISADTYVVFFERLK
                     DEVREGKSLRASVDRGWQRSFHTLVSANAVSFFAAVILYVLAVGPVRGFAFTLGLATV
                     IDFFAAWFFARPSVSLLTRLRLFQEGRFVGIRAAV"
     gene            complement(4224..4919)
                     /locus_tag="VFO47_02385"
     CDS             complement(4224..4919)
                     /locus_tag="VFO47_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET8787581.1"
                     /translation="MTLPERRAAAPELRLLDQVAGHQAIQTYIALADQFTGRLGYTEH
                     GFRHANLVGHIAYNILDRLGYDRHLAELAAVAGYLHDVGNVISRQNHPQTSALLALPV
                     LAELGMATEDIGLVIGAIGNHEEELGHPVSPVSAAVNIADKSDVHRSRVRKDARVEFD
                     IHDRVNYSATHSFVRVDATSRTITLELQIDTAVSDILEYFEIFLTRMMMCRRAAEGLD
                     CKFRMTVNGHSLL"
     gene            complement(4916..5230)
                     /gene="yajC"
                     /locus_tag="VFO47_02390"
     CDS             complement(4916..5230)
                     /gene="yajC"
                     /locus_tag="VFO47_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET8787582.1"
                     /translation="MEGSGSLVILVLMVAVFYFLLIRPQQRRLKALQSLQSSLQLGDE
                     IVTSAGFLGTIRRFDGEIVTIELSPGVEARVNRRAISGKVNPEPAGGTAEVADEEERP
                     ER"
     gene            complement(5270..6322)
                     /gene="ruvB"
                     /locus_tag="VFO47_02395"
     CDS             complement(5270..6322)
                     /gene="ruvB"
                     /locus_tag="VFO47_02395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET8787583.1"
                     /translation="MNRDDLLDPWPSDSERASEGSLRPRKLDEFVGQARVREQLGIVL
                     ESARRRGLPPDHLLFSGPPGLGKTSLAAIVAAELGVGFRVTSGPALERAGDLAAILTG
                     LEAGDVLFVDEIHRLPRAVEEVLYPAMEDFQIDVVLGKGPGARSLRLDLPRFTLVAAT
                     TRTGLITSPLRDRFGFSARLDYYGPADLQAIVTRSAAILGVAIDQAGAEVLARRSRGT
                     PRIANRLLRRVRDYAEVRADGSVDAAVAEAALELFEVDRLGLDKLDLALLRVLCERFG
                     GAPVGLATLAVSVGEEQDTVEDVAEPYLLQLGFLQRTPRGRVATAAAFAHLGLSPPRG
                     TGAAPSPGSLFEDRPE"
     gene            complement(6364..6954)
                     /gene="ruvA"
                     /locus_tag="VFO47_02400"
     CDS             complement(6364..6954)
                     /gene="ruvA"
                     /locus_tag="VFO47_02400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET8787584.1"
                     /translation="MIASLRGRLLEVLADGAVIEVGGVGYRVHLTPKAAAGLPRDGEV
                     LVHTVTYVREDTLALYGFATTDERRAFEQLLTATGVGPKLALAVLAVHSPDALRRAVS
                     AGDADALTLVPGVGRKGAARLILELKGKLGDGEPDLPVEPAGRPAYAEVREALGALGY
                     GPAEIKTALATLPADAGERPTEELLRLALRGLGSSR"
     gene            complement(6951..>7457)
                     /locus_tag="VFO47_02405"
     CDS             complement(6951..>7457)
                     /locus_tag="VFO47_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET8787585.1"
                     /translation="AGYGTISTAGEQVAPRLADLAARLREVLNAHRPEALAIERLFFN
                     ANVRTAMTVGQASGVVLLLAAEHGLEVTAYTPPQVKQAVTGSGSAPKEQVGYMVKALC
                     GLTSVPAPADTADALAVALCHLNHTGLAAPGAGPGGNGAALGSAPAADWLARAAPTSP
                     GLAKGAGR"
BASE COUNT         1022 a         2525 c         2873 g         1037 t
ORIGIN      
        1 gatgggccag atagggcacg ccagaggccc ggcgcttgcc gccgtggcgc ccgcgggcca
       61 gctcgaaggc gcggccaacg gtggcggtgt cggcccgggg gttgcgctgg cggacccgct
      121 tcagcagcgg ctcgacgtcc ggctcgggcg gcagtccggc gtcgtacggc cgggtgacgg
      181 cccccgcggt ggcgccgttc tccccgtcca cgccggcacc gggctggggc tcccgttcga
      241 gcgccagccc gtcggggacc gccgtcggat gctgctgcgc catcgtgctt ggccccaccc
      301 tggttggtcg tccgccgccg ggtcgtcttt gggggcgacc tgacggcgac taggctccca
      361 cggtactccc gccgtggcca gcctgccgat gctcggcccc cctggccatg gcctcgtcga
      421 ggacgacgtg gccgacgtag gcgtcgcgcc agccgaaggt ctcgaccgtc ttgtcctcca
      481 gggtcttgta gacgctgaag aagtgctcga tctcgcgcag caggtggatg gggacctgat
      541 cgatccggtg gatgtagttc cagtgggggt cggtcagggg gacgaccagc agcttctggt
      601 ccgggccctt gtcgtcggcc atgtcgagca tgccgacgat gcggcccttg atctcgcagc
      661 cggggaaggt tggctcaccc agcaggacca gggcgtccag ggggtcgccg tcctcggcca
      721 gggtgtcggg cacgaacccg tagtcggtcg ggtagtggac ggggctgaac agcatccggt
      781 cgaggcggat acggcccgtc tcgtggtcga gctcgtactt gttgcgagac cccttgggga
      841 tctcgacgac cacgtggatg gggagctcag taccggatga ggctgaagaa ctcatagcct
      901 tcgatcagcc tcctgccctc caggaactcg agctcgatca gggtggcgat gccgatcacc
      961 ttgcccccca ggcgctccac caaacgggcc gcggcccggg cggtgccgcc ggtggcgagc
     1021 acgtcgtcga cgatcaggac ccgctccccg ggggtgacgg cgtcggcgtg cagctccagg
     1081 gtggcggtgc cgtactcgag cgcgtactcc tcggcctcga cctggtaggg cagcttgccg
     1141 gccttgcgca cggggaggaa gccggccccg aagtggtagg cgaccggcga ggccaggatg
     1201 aagccgcggg cctcgatgcc gaccaccttg tcgacgttgc cgcggccgaa gtggaccacg
     1261 gtctcgtcga tcaccgagga gaaggcgacc tcgttcgcca gcagcggggt gatgtccttg
     1321 aagacgatcc ccggcttggg gaagtcgggc acgtcgcgga tgagggcccg caggcgctgg
     1381 atgtcgatgg gcacggtcgg ggcctcctcg tggggctagc gccggcggcg cttggccggg
     1441 cgggactgct ggcggccgga cggcttgcgc cgctgctggg tcgggcgtgg cttctggggc
     1501 tggcggcggg ccggcgtctg ggcgaccacg gccggctcgg ccggctcggc ctcggccacc
     1561 tcctccagct cctcgtcggg cggcagctcg ggatcggggg ccagctccgg ctccggcggc
     1621 ggggcggcgg cacggcgtga gcgggcgtcg cgggccgcgc cgccggcccg gaccgggcgt
     1681 gcggggcgct ccgggcgctc gggccgctcg gtcgcgccct gggcgcccag gcgctcgacc
     1741 cgggcccgga gctgggcgta gcggggctcc ttctccttcc acatggccac cagcggcggg
     1801 gcgatgaaga tggaggagta ggccccggcg gccacgccga cgaacagggc cagggccagg
     1861 tccttgaggg tctcggcgcc gagcaggtag ttgccgacca gcagcagccc caggatcggc
     1921 agcagcgacg ccagggaggt gttgagcgac cgcatcatcg tctggttgac ggccaggttg
     1981 gtcgcctggg agtaggtggt ccgcgacatc gaggtgagcg cgccggtgtt ctcccggacc
     2041 ttgtcgaaca ccaccacggt gtcgtagagc gagtacccga ggatggtcag cagggcgatc
     2101 acggtggccg gggtgacctc gaagcgggcc agggcgaaga tcccggcggt gatgatcagg
     2161 tcgtggagca gcgccaccag ggcggccacg gccatcttga actccatccg cagcgacacg
     2221 tagagggtca ccacgaccag gaacaccagc agggcgacga tcgccttgcg ggagacggtc
     2281 cccccccact tggagccgat ggtctgggtg gagacctggt tgcgctcgac cccgcccagc
     2341 tcggccagct cggtccggat cctggccacc tcggccggct ccagcacctc ggtctggacg
     2401 atcacctcga ccggctggct ctgggagatc tgcacgacct cggccaccgg cccgagcttg
     2461 tcgcggacct gggcctcggt gaccgcctcc tgcggctgga cccggaacac ggccccgccc
     2521 ttgaagtcga tgccgaagtt gagacccggc cagatcaggc tgacgatcga gatcagcagg
     2581 agcacggccg agatcgagaa ccagaggcgc cggcgaccga cgatgtcgta gctggtctcg
     2641 ccgtggtaca ggcggctggc gagggacacg gacgggatcc tccttgcggg gtcagacggc
     2701 ggcgcggatg ccgacgaagc gcccctcctg gaacaggcgg agtcgggtga gcagggagac
     2761 cgacggccgg gcgaagaacc aggcggcgaa gaagtcgatc acggtggcca ggccgagggt
     2821 gaacgcgaag ccccgcaccg ggccgacggc cagcacgtac aggatcacgg cggcgaagaa
     2881 cgacaccgcg ttggccgaga ccagggtgtg gaacgagcgc tgccagcccc ggtcgacgga
     2941 ggcgcgcagg gacttgccct cacggacctc gtccttcagc cgttcgaaga agaccacgta
     3001 ggtgtcggcg gagatgccga cggccacgat caggccggcg atcccggcca gggtcagggt
     3061 gaagccgatc agctcgccca tgacgatgac gatgccgacg ttgagggcgg ccgcgatgac
     3121 caggccgacc cagatcacca ggcccaggcc gcggtagtag acgagcacgt acaggagcac
     3181 caggccgagc ccgacggccc cgccgatgag gccggcccgc agcgagtcgg cccccagggt
     3241 ggcgctgacg gtggtgcggg tctgcgggtc gagcttgagg ggcagggcgc ccgaggagat
     3301 cagcggggcc aggttctcgg cctcggcctg ggtctggccg gtgatctcgg cctggccgcc
     3361 gctgatgccc tggtcgcact ggatgctctc ggccaccggc gggctgttga tcacgaccgc
     3421 gtccagggtg atggcgatct ggcgcttgac gtcgcccacc tggttgcagg ccgccttgcc
     3481 ggtgagctcc tcgaacttgt cgccgccgcg gccgctgaag ttgagggccg tcacccactc
     3541 gccggtggtc accccgccct ggccggtctg ggccagctgg gcctgggccg aggcgacgtc
     3601 ggtgccgccg agggcgaccg gccccatcag cagcttggcc gagttggcgt cgggcgccag
     3661 ccgaccctcg gtccgggagc agaggaccac gtccttgtcg gggacgtcct tggtcgagta
     3721 ggtctcgggc ttggcgcagt cgacggcctt gaactcgccc tggttgtact tggggtcgtt
     3781 cggcgccacg acctccttga ccgggcggaa ctggagctgg gcggtggtgc cgacggcggc
     3841 gatgacctcg tcggggttgg gcacgtcggg gatggacacc acgatgttgc cgccctcgag
     3901 gttgacctcg gactcggcca cgcccttgga gttcacccgg ctgcggatga tgtcgacggt
     3961 ggtgtcgagc tgctgctggg tcggctcgcc gccccgcggg ttggtcggca cgaaggtgac
     4021 ggtggacccg ccgcgcaggt cgaggccgag ccggggctcc agcccctgga ccaggaccac
     4081 cgcccagacc acgccgacca gtgcgaacac gaccagcagc gggatgacat agcgacgggt
     4141 tcctggcacg gacctgtggc tccttcgtgc tgcaaggggt tcgaaacaga gagcctagcg
     4201 gttttctccc gcgttcgagc gggctagagc aggctgtggc cgttgacggt catgcggaac
     4261 ttgcagtcca ggccctcggc ggcccggcgg cacatcatca tgcgggtcag gaagatctcg
     4321 aagtactcga ggatgtcgga caccgccgtg tcgatctgga gctccagggt gatggtgcgg
     4381 ctggtggcgt cgacccgcac gaacgagtgg gtggccgagt agttcacccg gtcgtggatg
     4441 tcgaactcga cccgcgcgtc cttgcgtacc cgggagcggt ggacgtcgga cttgtcggcg
     4501 atgttgaccg ccgccgacac cggcgacacg gggtggccga gctcctcctc gtggttgccg
     4561 atggccccga tgaccaggcc gatgtcctcg gtggccatgc ccagctcggc cagcacgggc
     4621 agggcgagca gggccgaggt ctgggggtgg ttctggcgcg agatcacgtt gccgacgtcg
     4681 tgcaggtagc cggcgacggc ggccagctcg gccaggtgcc ggtcgtagcc gagccggtcg
     4741 aggatgttgt aggcgatgtg gccgaccagg ttggcgtgcc ggaagccgtg ctcggtgtag
     4801 cccagcctcc cggtgaactg gtcggcgagg gcgatgtagg tctggatggc ctggtggccg
     4861 gccacctggt cgagcaggcg gagctcgggg gccgcggccc ggcgctccgg gagggtcatc
     4921 gttcgggccg ctcctcttcg tcggcgacct cggccgtccc gcccgccggc tcggggttga
     4981 ccttgcccga gatggcccgg cggttgaccc gggcctcgac gcccggggac agctcgatgg
     5041 tgacgatctc cccgtcgaaa cggcggatgg tgccgaggaa ccctgccgag gtgacgatct
     5101 cgtcccccag ctgcagcgag gactgcagcg actgcagcgc cttgaggcgg cgctgctggg
     5161 gacggatgag caggaagtag aagacggcga ccatcagcac gaggatgacg agtgacccgc
     5221 taccttccat ggatttccga cctccggggg tgccgatgcg gcggcaatcc tactccggcc
     5281 ggtcctcgaa gagggagccc ggagacgggg cggctcccgt gccccggggc gggctgaggc
     5341 cgaggtgggc gaaggcggcc gcggtcgcca cccggccgcg gggggtccgc tgcaggaagc
     5401 cgagctggag caggtagggc tcggccacgt cctcgaccgt gtcctgctcc tcgccgaccg
     5461 acacggccag ggtggccagg ccgaccgggg cgccgccgaa ccgctcgcac agcacccgca
     5521 gcagggccag gtcgagcttg tccaggccga ggcggtcgac ctcgaacagc tcgagggcgg
     5581 cctcggccac ggccgcgtcc accgacccgt cggcccggac ctcggcgtag tcgcggaccc
     5641 ggcgcagcag ccggttggcg atccgcgggg tgccccgtga ccgcctggcc aggacctcgg
     5701 ccccggcctg gtcgatggcc accccgagga tggccgccga gcgggtgacg atggcctgca
     5761 ggtcggccgg cccgtagtag tcgagccggg ccgagaagcc gaagcggtcg cgcagcggcg
     5821 aggtgatcag cccggtgcgg gtggtggcgg cgaccagggt gaagcggggc aggtcgaggc
     5881 gcaggctgcg cgcccccggc cccttgccga gcacgacgtc gatctggaag tcctccatgg
     5941 ccgggtagag gacctcctcg accgcccggg ggaggcggtg gatctcgtcc acgaacagca
     6001 cgtcccccgc ctccaggccg gtgaggatgg cggccaggtc gccggcccgc tccagggccg
     6061 ggccggaggt gacccggaag ccgaccccca gctcggcggc cacgatcgcg gccaggctgg
     6121 tcttgcccag gcccgggggc ccggagaaca gcaggtggtc ggggggcagg ccgcgccggc
     6181 gggccgactc gagcacgatc ccgagctgct cgcggaccct ggcctggccg acgaactcgt
     6241 ccagcttgcg cgggcgcagg ctgccctcgc tggcccgctc ggagtccgag ggccaggggt
     6301 cgaggaggtc gtctctgttc atcggggcac ccccctgggg ttccccgaac ccctccgtgt
     6361 cggtcaccgg ctcgagccca atccgcgcag ggcgagccgc aggagctcct cggtcgggcg
     6421 ttcgccggcg tcggccggga gggtggccag ggcggtcttg atctcggccg gcccgtagcc
     6481 gagggcgcca agggcctcgc ggacctcggc gtaggccggg cggccggccg gctcgaccgg
     6541 caggtcgggc tcgccgtcgc ccagcttgcc tttgagctcc aggatcagcc gggccgcgcc
     6601 cttgcgcccg accccgggga ccagggtcag ggcgtcggcg tcgccggcgc tcacggcccg
     6661 gcgcagggcg tcgggggagt gcacggccag cacggccagg gcgagcttgg gcccgacccc
     6721 ggtggcggtc agcagctgct cgaaggcgcg gcgctcgtcg gtggtggcga agccgtacag
     6781 ggccagggtg tcctcgcgca cgtaggtgac ggtgtggacc agcacctcgc cgtcgcgggg
     6841 cagcccggcg gccgccttgg gggtcaggtg cacccggtac ccgaccccgc cgacctcgat
     6901 cacggccccg tcggccagga cctcgagcag ccgcccgcgc aggctggcga tcaccgtccg
     6961 gcccccttgg cgagcccagg ggaggtcggg gcggcccggg ccagccagtc ggcggccggg
     7021 gccgacccga gggcggctcc attgccgccg ggcccggcgc cgggagcggc caggccggtg
     7081 tggttgaggt ggcacagggc cacggccaag gcgtcggcgg tgtcggccgg ggccgggacc
     7141 gaggtcagcc cacagagcgc cttgaccatg tacccgacct gctccttggg cgcgctcccg
     7201 ctgccggtga cggcctgctt gacctgcggc ggggtgtagg cggtgacctc gaggccgtgc
     7261 tcggcggcca gcagcagcac cacccccgac gcctggccga cggtcatggc cgtgcgcacg
     7321 ttggcgttga agaacagccg ctcgatggcc agcgcctccg gccggtgggc gttcagcacc
     7381 tcccgcagcc gggccgccag gtcggccagg cgcggagcca cctgctcgcc ggcggtggag
     7441 atggtgccgt agccggc
//
LOCUS       DASSWR010000146         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_909370,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000146 DASSWR010000000
VERSION     DASSWR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909370"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFO47_02410"
     CDS             <1..324
                     /locus_tag="VFO47_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8787586.1"
                     /translation="GGLLTGEAAGLDLDRLSGASLAAFAYLVVAGSLVAYTAYVWLLA
                     NAPVSQATTHTHVNPVVAVFLGWLILHERVTLATLAGAALIIGSVAVVVRHESAAEPE
                     PETVP"
     gene            470..607
                     /locus_tag="VFO47_02415"
     CDS             470..607
                     /locus_tag="VFO47_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787587.1"
                     /translation="MQLKELTVWWQQRRIAREGRAAFESAATRELYEALDEASMLAPR
                     R"
     gene            676..1572
                     /locus_tag="VFO47_02420"
     CDS             676..1572
                     /locus_tag="VFO47_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET8787588.1"
                     /translation="MAHEHRHSAAAEHRGRLAVVLAITVAVLVAEVAGAALSGSLALL
                     ADAGHVLADGAGIGLALLAIRFAARPATPQRTFGYYRLEILAAVVNAVLLFGVAGFVL
                     VEAWRRLTDPPAVASGLMLAVAAAGLVANAVSLWLLHGGQRDSLNLRGAYLEVWGDLL
                     GSVAVLAAAAVIAVTGFQAADPIASALIGVAILPRTWRLLREAVDVLLEATPKGVDMD
                     EVRRHLLETPGVTDVHDLHAWTITSGLPVLSAHVVLERHADAGKVLDGLGECLAGHFD
                     IEHSTFQLEQPEHRGHEGATHR"
     gene            1578..>2071
                     /locus_tag="VFO47_02425"
     CDS             1578..>2071
                     /locus_tag="VFO47_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8787589.1"
                     /translation="MPTRRLGGLQVSAIGLGEMPMSLAGRPDEDRSIRTVHAALDAGV
                     TLVDTADAYCIDGSEMGHGERLVAKALAAWPGDRDRVLVATKGGHTREGGEWHLDGRP
                     EYLRQACEASLAALGVEAIGLYQFHRPDPKVPFAESVGAMAELKAAGKVRLVGLSNVS
                     VDQI"
BASE COUNT          255 a          795 c          742 g          279 t
ORIGIN      
        1 ggcggcctgc tcaccggcga ggccgccggc ctggacctgg accgcctctc gggcgcgtcc
       61 ctggccgcct tcgcctacct ggtggtggcc ggctcgctgg tcgcctacac cgcctacgtg
      121 tggctgctgg ccaacgcccc cgtgtcccag gccaccaccc acacccacgt caacccggtg
      181 gtggccgtct tcctgggctg gctgatcctc cacgagcgcg tcaccctcgc caccctggcc
      241 ggcgccgccc tgatcatcgg ctcggtggcc gtggtcgtcc gccacgagtc ggcggcggag
      301 ccagagcccg agaccgttcc gtgatggatc ggggtcgcaa cggtgacccg cagcatgccg
      361 gtttgttacc tacggcggcg agactacgcc ctgtcgatgc gccgcaaccg acaggcggcg
      421 agggcgacgc gggctggggg gttcgcgtcg gggcagcagg agggggacaa tgcagctcaa
      481 ggaactcacc gtctggtggc agcagcgcag gatcgcccgg gagggtcgtg ccgcattcga
      541 gtcggccgcc acccgcgagc tctacgaggc gctcgacgag gcctcgatgc tggcgccgag
      601 gcgctgacgc gcaacgaccc tgacggcccg ggcgatcgcc cgggccgttc ggttgtgctg
      661 tggcagagtg accccatggc acacgagcac cgtcacagcg ccgccgccga gcaccggggc
      721 cggctggcgg tggtgctcgc gatcaccgtg gccgtgctcg tggccgaggt cgccggggcc
      781 gccctcagcg gcagcctggc cctgctcgcc gacgccgggc acgtgctggc cgacggcgcc
      841 gggatcggcc tggccctgct ggcgatccgg ttcgcggcac gcccggccac cccgcagcgg
      901 accttcggct actaccggct ggagatcctg gccgccgtgg tcaacgccgt gctgctgttc
      961 ggcgtggccg ggttcgtgct ggtcgaggcg tggcggcgcc tgaccgaccc gcccgcggtg
     1021 gccagcgggc tcatgctggc cgtggccgcc gccgggctgg tcgccaacgc cgtctcgctg
     1081 tggctgctcc acggcggcca gcgggacagc ctgaacctgc gcggcgccta cctggaggtc
     1141 tggggcgacc tgctcggttc ggtggcggtc ctggccgccg ccgccgtgat cgccgtcacc
     1201 ggcttccagg ccgccgaccc gatcgcctcg gccctgatcg gcgtggccat cctgccccgg
     1261 acctggcggc tgctccgcga ggcggtggac gtcctgctgg aggccacccc caagggggtc
     1321 gacatggacg aggtccgccg gcacctgctc gagaccccgg gcgtgaccga cgtccacgac
     1381 ctgcacgcct ggaccatcac ctccggcctg ccggtgctgt cggcccacgt ggtcctggag
     1441 cgccacgccg acgccggcaa ggtcctcgac gggctcggcg agtgcctggc cggccacttc
     1501 gacatcgagc actccacctt ccagctcgag cagcccgagc accgcggcca cgaaggagcg
     1561 acccaccggt gaccaccatc ccgacccgcc ggctgggcgg gctccaggtc agcgccatcg
     1621 ggctcggcga gatgccgatg tcgctggccg gccggcccga cgaggaccgc tccatccgca
     1681 ccgtccacgc cgccctcgac gccggcgtga ccctggtcga caccgccgac gcctactgca
     1741 tcgacgggtc cgagatgggc cacggcgagc gcctggtcgc caaggccctg gccgcctggc
     1801 cgggcgaccg cgaccgggtg ctggtggcca ccaagggcgg ccacacccgc gagggcggcg
     1861 agtggcacct ggacgggcgg cccgagtacc tgcgccaggc ctgcgaggcc agcctggccg
     1921 ccctcggggt cgaggccatc ggcctctacc agttccaccg gcccgacccc aaggtcccgt
     1981 tcgccgagtc ggtcggggcc atggccgagc tcaaggcggc cggcaaggtc cggctggtcg
     2041 gcctctccaa cgtcagcgtc gaccagatca a
//
LOCUS       DASSWR010000147         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_824561,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000147 DASSWR010000000
VERSION     DASSWR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824561"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VFO47_02430"
     CDS             complement(<1..1311)
                     /locus_tag="VFO47_02430"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET8787590.1"
                     /translation="MTTIPSNLRRVLRREPRVRPTTEPVAAPPERVEAPPVEIAPNDP
                     LVAYFSGATGAVDIGALQLDSPALEGLREAGIKLVVPLVSQGELIGLLNLGPRRSEQE
                     YSADDRKLLNDLAGHAAPAVRVAQLVREQEAEVRQRERIEQELRVAQLIQQQFLPQEL
                     PDLPGWQVGAYYGPAREVGGDFYDFIKLPDDQIGIVVGDVTDKGVPAALVMATTHSIL
                     RAEAPRLVAPSEVLTRANELLVDEMPAHMFVTCLYAVLDPASGRLRYANAGHNVPYVA
                     TEDGVTELRATGMPLGLLPEMTYEEKETTLALGDTVLLHSDGLAEAHNARREMFGFPR
                     VAALTGEASDGEVLIDRLLKELQEFVGPDLEQEDDITLVTLGRRTAGAPAAGGNQPAA
                     RELAAFEVASEEGNERLAIDQVAEAVAGLGLDGARLERLKTAVGE"
     gene            1431..>2348
                     /locus_tag="VFO47_02435"
     CDS             1431..>2348
                     /locus_tag="VFO47_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8787591.1"
                     /translation="MAELRIALLGPPRVERDGAPIEVDTRKAIALLAYLALHPERHGR
                     DAVAGLLWPDYDTEHARGALRRTLSTLNKAVGPGWLAADRTTVGFARTDFWLDVARFE
                     DLLAACRDHGHAPADACPACVAPLAEAAGLHRGDFLAGFGLRDSTSFDDWQYFQAERL
                     RRELAGALERLATARIGQHDWDGAVDAARRWLVLDPLHEPAHRQLMRIYAWSDRRGAA
                     LRQFQACQRVLEEELGVEPLEETVAVHEAIQANRLPPPPPPTPAPATAPAPTPTPAPA
                     TAPAPATLGAPSAPASAATPAAEPRDAGAP"
BASE COUNT          285 a          895 c          868 g          300 t
ORIGIN      
        1 ctcgccgacg gccgtcttga gccgctccag gcgggcgccg tccaggccca ggccggcgac
       61 ggcctcggcc acctggtcga tggccaggcg ctcgttgccc tcctcgctgg ccacctcgaa
      121 ggcggccagc tcgcgcgcgg ccggctggtt cccgcccgcg gccggcgcgc cggcggtccg
      181 gcggcccagg gtgaccaggg tgatgtcgtc ctcctgctcc aggtcgggac cgacgaactc
      241 ctgcagctcc ttgagcagcc ggtcgatcag cacctcgccg tcggaggcct cgccggtcag
      301 ggcggccacc cgggggaagc cgaacatctc ccgccgggcg ttgtgggcct cggccagccc
      361 gtcggagtgg agcagcaccg tgtcgccgag ggcgagggtg gtctccttct cctcataggt
      421 catctccggc agcagcccca gcggcatgcc ggtggcccgc agctcggtca ccccgtcctc
      481 ggtggcgaca taggggacgt tgtggccggc gttggcgtag cgcagccggc cgctggccgg
      541 gtccagcacc gcgtacaggc aggtgacgaa catgtgcgcc ggcatttcgt ccaccagcag
      601 ctcgttggcc ctggtgagga cctcgctggg ggcgaccagc cggggtgcct cggcccgcag
      661 gatcgagtgg gtggtggcca tgaccagcgc cgccgggacg cccttgtcgg tgacgtcgcc
      721 gaccacgatg ccgatctggt cgtcgggcag cttgatgaag tcgtagaagt ccccgccgac
      781 ctcgcgggcc ggcccgtagt aggcgccgac ctgccagccg ggcaggtcgg gcagctcctg
      841 gggcaggaac tgctgctgga tcagctgggc gacccgcagc tcctgctcga tccgctcacg
      901 ctggcggacc tcggcctcct gctcgcggac gagctgggcg acccgcaccg ccggggcggc
      961 gtggccggcc aggtcgttga gcagcttgcg gtcgtcggcc gagtactcct gctcgctgcg
     1021 ccgggggccc aggttgagca ggccgatcag ctcaccctgg ctgaccaggg ggacgaccag
     1081 cttgatgccg gcctcgcgca ggccctcgag ggcgggcgag tccagctgca gcgcgccgat
     1141 gtcgaccgcc ccggtcgccc ccgagaagta ggcgaccagc gggtcgttgg gcgcgatctc
     1201 gaccggcggc gcctcgaccc gctcgggcgg cgcggccacc ggctcggtcg tcggccggac
     1261 ccgcggctcg cgccgcagga cccgccgcag gttgctcggg atcgtggtca tggcacctcc
     1321 tcgagggcgg ttgtaccgtc ccaccgctcc ggcatcgtta cggactgcgt cacgagacgt
     1381 gaattctggg accatactga ccgcgtggcg tggcgacgag gagggggtgc atggcggagc
     1441 tgcggatcgc gctgctgggg ccgccgcggg tcgagcggga cggggcgccg atcgaggtcg
     1501 acacccgcaa ggcgatcgcg ctgctcgcct acctggccct gcaccccgaa cggcacggcc
     1561 gcgacgccgt cgccggcctc ctctggcccg actacgacac cgagcacgcc cggggggcgc
     1621 tgcggcgcac cctgtcgacc ctgaacaagg ccgtggggcc ggggtggctg gcggccgacc
     1681 ggacgacggt ggggttcgcc cggacggact tctggctgga cgtggcccgg ttcgaggacc
     1741 tgctggccgc ctgccgggac cacgggcacg cgccggccga cgcctgcccg gcctgcgtgg
     1801 ccccgctggc cgaggcggcc gggctgcacc ggggcgactt cctggccggg ttcggcctgc
     1861 gcgacagcac cagcttcgac gactggcagt acttccaggc cgagcggctg cggcgcgagc
     1921 tcgcgggggc gctggaacgc ctggccacgg cccggatcgg ccagcacgac tgggacgggg
     1981 cggtcgacgc cgcccgccgc tggctggtcc tggacccgct gcacgagccg gcccaccgcc
     2041 agctcatgcg catctacgcc tggagcgacc ggcgcggggc cgccctgcgc cagttccagg
     2101 cctgccagcg ggtcctggag gaggagctcg gggtcgagcc ccttgaggag acggtcgccg
     2161 tccacgaggc gatccaggcc aaccgcctcc caccaccccc gcccccaacc ccagccccgg
     2221 ccacggcccc ggccccaacc ccaaccccgg ccccggccac ggccccagcc ccggccaccc
     2281 tgggggcacc gtcggccccg gcatccgcgg ccacgccggc agccgaaccc cgggatgccg
     2341 gggccccc
//
LOCUS       DASSWR010000148         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_175514,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000148 DASSWR010000000
VERSION     DASSWR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175514"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFO47_02440"
     CDS             <1..1024
                     /locus_tag="VFO47_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787592.1"
                     /translation="ALLGPSGCGKTTTLRMVAGLEEPSDGQITLGEEDITRLRPYRRP
                     VNTVFQNYALFPHLSIFENVAFGLRRRGAKDVKRQVQEMLELVELEQYAGRKPAQLSG
                     GQQQRVALARALINRPQVLLLDEPLGALDLKLRRQMQIELKRIQTEVGITFVHVTHDQ
                     EEAMTMADTIAVMNSGVIEQMGNPLDLYEHPATTFVSNFLGQSNLVTGSVLQSQPDHL
                     LVDVEGTKLLAPASRARSTEGRVWVGVRPEKVFLAHAGSETDDGSNALRGGVVTDVSF
                     VGVSTQYLARLPWGQELMIFEQNPGARESFRPGDQVDLHWMPDHTFLLDAAQDAEAGV
                     EKEGVE"
     gene            1108..1926
                     /locus_tag="VFO47_02445"
     CDS             1108..1926
                     /locus_tag="VFO47_02445"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787593.1"
                     /translation="MLLLPGALWLLMFFVIPTITLVSTSLYDPSGSIITGYKMTFAWQ
                     NYVDGITGYGAQLIRSFVYAGIATLACIVLGYPLAYAIAFKAGRWKNLMLVMVIAPFF
                     TSFLVRTLSWQLLLADNGWAVGVLQALHILGPDGHLLATPFAVVTGLTYNFLPFMVLP
                     LYASLEKIDHRLIEAGSDLYASPATTFRKVTLPLSMPGLVAGTLLTFIPAAGDYINAE
                     LLGTPTTRMVGNEIQDLFAAGDYPTASALSVTLMAAIVVLVLVYVRRSGAEDLV"
BASE COUNT          309 a          721 c          669 g          323 t
ORIGIN      
        1 cgccctgctc ggcccctcgg gctgcggcaa gaccacgacg ctgcggatgg tggccggcct
       61 ggaggagccc agcgacggcc agatcacgct cggcgaggag gacatcaccc ggctgcggcc
      121 ctaccgtcgg ccggtcaaca ccgtgttcca gaactacgcg ctcttcccgc acctgagcat
      181 cttcgagaac gtcgccttcg ggctgcgtcg ccgtggcgcc aaggacgtca agaggcaggt
      241 gcaggagatg ctcgagctgg tcgagctcga gcagtacgcc gggcgcaagc ccgcccagct
      301 ctccggtggg cagcagcaac gcgtcgccct ggcgcgcgcg ctcatcaacc ggccgcaggt
      361 cctgctgctc gacgagccgc tcggggcgct cgacctcaag ctgcgccgcc agatgcagat
      421 cgagctcaag cggatccaga ccgaggtggg gatcaccttc gtgcacgtga cccacgacca
      481 ggaggaggcc atgaccatgg ccgacaccat cgcggtcatg aacagcgggg tgatcgagca
      541 gatgggcaac cccctcgacc tctacgagca cccagccacc acgttcgtgt ccaacttcct
      601 ggggcagtcg aacctggtga ccgggtcggt gctgcagagc cagcccgacc acctgctcgt
      661 cgacgtcgag ggcaccaagc tgctcgcgcc cgcctcacgc gcgcgctcga ccgagggacg
      721 ggtgtgggtc ggcgtacggc cggagaaggt cttcctcgcc cacgccggca gcgagaccga
      781 cgacgggtcc aacgcgctgc gtggcggggt ggtcaccgac gtcagcttcg tcggggtcag
      841 cacgcagtat ctcgcccggc ttccctgggg ccaggagctc atgatcttcg agcagaaccc
      901 cggtgcccgc gagtccttcc ggcccggcga ccaggtcgac ctgcactgga tgcccgacca
      961 caccttcctc ctcgacgccg cccaggacgc ggaggcggga gtggagaagg agggcgtcga
     1021 gtgagcatcg cggccgccca cgtcgggccg ggtgagcagc aggtctccgg cgggcagaag
     1081 gagcaaaagc gcggctggac cggctacgtg ctgctcctgc ccggcgcgct gtggctgctg
     1141 atgttcttcg tgatccccac gatcaccttg gtgagcacga gcctgtacga cccgtcgggc
     1201 tcgatcatca ccggctacaa gatgaccttc gcctggcaga actacgtcga cggcatcacc
     1261 gggtacggcg cccagctgat ccgctccttc gtctacgccg ggatcgcgac gctcgcctgc
     1321 atcgtgctcg ggtaccccct ggcctacgcc atcgccttca aggccgggcg gtggaagaac
     1381 ctgatgctgg tgatggtgat cgcccccttc ttcaccagct tcctcgtgcg caccctgtcg
     1441 tggcagctgc tgcttgccga caacggctgg gccgtcggcg tcctccaagc cctgcacatc
     1501 ctcggccccg acggccacct gctggccacg ccgttcgcgg tcgtgaccgg gctgacctac
     1561 aacttcctgc cgttcatggt gctcccgctc tacgcgagcc tggagaagat cgaccaccgg
     1621 ctcatcgagg ccggcagcga cctctacgcc tccccggcca ccaccttccg caaggtcacc
     1681 ttgccgctgt cgatgcccgg gctggtggcg ggcacgctgc tgaccttcat cccggcggcg
     1741 ggcgactaca tcaacgccga gctgctcggc acgccgacca cgcggatggt cggcaacgag
     1801 atccaggacc tcttcgcggc cggcgactac ccgacggcct cggcgctgtc ggtgacgctg
     1861 atggcggcca tcgtcgtgct ggtgctggtc tacgtacgcc gctccggggc cgaggacctg
     1921 gtgtgagcgc cctgacgcgc tggtgggggc gccacgtcgt cctggtcatg gggctgctgg
     1981 tgctggccta catgatggtg ccgatcgtcg tcgtggtgct ga
//
LOCUS       DASSWR010000149         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_543157,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000149 DASSWR010000000
VERSION     DASSWR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543157"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFO47_02450"
     CDS             complement(<1..711)
                     /locus_tag="VFO47_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HET8787594.1"
                     /translation="MGGGDWAEVTARAAAEGQDDVMIINMGPQHPSTHGVLRLVVELK
                     GEEVINLQPVVGYLHTGIEKSTEFRTWVQGVTFVTRMDYLAPIFNETAYCLAVEKLLG
                     IEAPPRAQALRVLMMELNRISSHLVWLATTGLELGALSVMLYGFREREEILDILEDVT
                     GLRMNHAWVRPAGVSQDLPEGTEDKIRAFVRDMRSKADEYETLLTNNPIWLERTRGIG
                     HLTLDELIGLGTTGPMLRA"
     gene            complement(861..1460)
                     /locus_tag="VFO47_02455"
     CDS             complement(861..1460)
                     /locus_tag="VFO47_02455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET8787595.1"
                     /translation="MTEPTATGTTEAPESGFQPPAALQPVLTGLRERFEDAIKEISGH
                     RGEVVVELERDRLVEVCTWLRDDPATSFRLLASITGIDYLKYDDRPERLCADYNLYSF
                     DHNLRLRLKVRVTVEDPHIPSVTGVWPGANFPEREAYDFFGIRFDGHPNLVRIHMPED
                     WKGYPQRKDYPLGGVDVEYIGAHVPPPDTRRYKGGWTST"
     gene            complement(1505..1966)
                     /locus_tag="VFO47_02460"
                     /pseudo
     CDS             complement(1505..1966)
                     /locus_tag="VFO47_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(1969..2331)
                     /locus_tag="VFO47_02465"
     CDS             complement(1969..2331)
                     /locus_tag="VFO47_02465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET8787596.1"
                     /translation="MNLGAFAPILAMLVLASAFAVLSIVVSAFLAPHRPTRAKYSAYE
                     SGIEPGLLPRGERYPVKFYLTAMLFVVFDVEVIFLYPWAVVFRQLGLFGLVQMGIFVG
                     LILVAYIYDWKRGGLEWD"
     gene            complement(2420..>3346)
                     /locus_tag="VFO47_02470"
     CDS             complement(2420..>3346)
                     /locus_tag="VFO47_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8787597.1"
                     /translation="DQLLARTASKAGADLWEATDVVEPLVENGLVRGAVVRREGEDPV
                     DVRAEVVVAADGASSRFAQALGLVRDEHRPIGVAIRQYYRSERDSDPWIDSYLELWKG
                     DHLLPGYGWVFPLADGTVNVGCGLLNTSEGFRNTNYRDLLKGWLPTVGPEWGFTLEDA
                     VAKPRSASLPMGASRHPPLYRGVLFVGDAAGLVNPFNGEGIDYAMESGRLAAEVGLEV
                     LESGDRTRLAAYRTALERGFGSYFTLGRVFVRAIGHPAVMKACARYGLPRPTLMKLVL
                     KLMANLYEPTRGDATDRIVRALVRLAPSRGGS"
BASE COUNT          494 a         1163 c         1188 g          501 t
ORIGIN      
        1 cgcccgcagc atcggcccgg tggtgccgag gccgatcagc tcatccaggg tcaggtggcc
       61 gatgcccctg gtccgctcca gccagatcgg gttgttggtg agcagggtct cgtactcgtc
      121 ggccttgctt cgcatgtcgc ggacgaaggc gcggatcttg tcctcggtgc cctcgggcag
      181 gtcctgggag accccggccg gccgcaccca ggcgtggttc atgcgcagcc cggtgacgtc
      241 ctcgaggatg tcgaggatct cctcccgctc ccggaagccg tagagcatga ccgacagggc
      301 gccgagctcg agcccggtgg tggccagcca gaccaggtgg gacgagatcc ggttgagctc
      361 catcatcagg acccgcaggg cctgggcccg gggcggcgcc tcgatcccca gcagcttctc
      421 gacggccagg cagtaggcgg tctcgttgaa gatcggggcc aggtagtcca tgcgggtcac
      481 gaaggtgacc ccctggaccc aggtgcggaa ctcggtcgac ttctcgatgc cggtgtgcag
      541 gtagccgacc accggctgga ggttgatgac ctcctcgccc ttgagctcca cgaccagccg
      601 cagcaccccg tgggtcgagg ggtgctgggg gcccatgttg atgatcatga cgtcgtcctg
      661 gccctcggcc gcggccctgg ccgtgacctc ggcccagtcg ccgccgccca tggccaccac
      721 ctggtggacg cgccgctcgt tcatggacac gtgctcggcc gcctgggccg ggggcaggtc
      781 gtgggcctcg ccggggcgga ccacgacctc gtcgaccagc tcgctggtcg gccggtcgat
      841 gtccggcgtc tcgacctcgc tcatgtgctg gtccagcctc ccttgtaccg cctggtgtcc
      901 ggcgggggga cgtgggcgcc gatgtactcg acgtcgaccc cgcccagcgg gtagtccttg
      961 cgctgcgggt agcccttcca gtcctcgggc atgtggatgc ggaccaggtt ggggtggccg
     1021 tcgaagcgga tgccgaagaa gtcgtaggcc tcccgctcgg ggaagttcgc ccccggccag
     1081 accccggtga ccgacgggat gtgcgggtcc tcgacggtga cccggacctt gagccggagc
     1141 cggaggttgt ggtcgaagga gtagaggttg tagtcggcgc agagccgctc gggccggtcg
     1201 tcgtacttca ggtagtcgat cccggtgatg ctggccagca gccggaagct ggtcgccggg
     1261 tcgtcgcgca gccaggtgca gacctcgacc agccggtcgc gctccagctc gaccacgacc
     1321 tcgccccggt gcccgctgat ctccttgatg gcgtcctcga agcgctcgcg cagcccggtc
     1381 aggacgggct ggagggccgc gggcggctgg aagccgctct ccggggcctc ggtcgtcccg
     1441 gtggcggtcg gctcggtcac ctggccgcct cttccaggac cagcttggag cggggctcgc
     1501 ccttcatgat cttctcgtgg agcctgagga tgccgtcgat cagcatctcc ggccggggcg
     1561 ggcagccggg cacgtacatg tccaccggca cgatctggtc gacgccctgg acgatcgcgt
     1621 agttgttgaa catgccgccc gaggtggcgc agacgcccat ggagatgacc cacttgggct
     1681 cgggcatctg gtcgtagatg gtccgcagca ccggggccat cttctgcgac acccggccgg
     1741 acacgatcat caggtcggcc tggcggggcg aggcccggaa cacctccatg ccgaaccggg
     1801 ccaggtcgta gcggccggcg ccggtggcca tcatctcgat ggcgcagcag gccagcccga
     1861 aggtggccgg ccacagcgag cccttgcggg cccagttggc cagcttctcc acggtcgtga
     1921 cgaggatgcc gcctggcagc ttgtccagtc cgctcccgct ctgcatggtc agtcccactc
     1981 caatccgccg cgcttccagt cgtagatgta ggccaccagg atcagtccga cgaagatgcc
     2041 catctggacc agtccgaaga ggccgagctg gcggaacacg accgcccacg ggtagaggaa
     2101 gatcacctcg acgtcgaaga ccacgaacag catcgcggtc aggtagaact tgaccgggta
     2161 gcgctcgccc cgcggcagca gccccggctc gatgccggac tcgtaggccg agtacttggc
     2221 ccgggtcggc cgatgggggg ccaggaacgc cgacacgacg atcgacagga ccgcgaacgc
     2281 gctggccagc acgagcatgg ccaggatggg agcgaaggca ccgaggttca cgggcagcct
     2341 ccaacagacc tcgagcgtgg ggccgacggc ggcagggcca gcctatggcc gcctcgggag
     2401 agcacgcaaa cctgggcggt cacgaccctc ccctcgaagg agccagtcgg accagggccc
     2461 gcacgatccg gtcggtggcg tcgccccggg tcggctcgta caggttggcc atcagcttca
     2521 ggaccagctt catcagcgtc gggcggggca gcccgtacct ggcgcaggcc ttcatcaccg
     2581 cggggtggcc gatggcccgg acgaacaccc ggccgagggt gaagtaggag ccgaacccgc
     2641 gctccagcgc cgtccggtag gcggccagcc gggtgcggtc gccgctctcc agcacctcaa
     2701 ggccgacctc ggcggccagc cggcccgact ccatggcgta gtcgatgccc tcgccgttga
     2761 acgggttgac cagcccggct gcgtccccca cgaacagcac gccccggtac aggggagggt
     2821 ggcggctggc ccccatgggc agggacgccg agcgcggctt ggccacggcg tcctccaggg
     2881 tgaaccccca ctcgggcccg accgtcggca gccacccctt gagcaggtcc ctgtagttgg
     2941 tgttgcggaa gccctcggag gtgttgagca gcccgcagcc gacgttgacg gtcccgtcgg
     3001 ccagggggaa cacccagccg tagccgggca gcaggtggtc gcccttccac agctccaggt
     3061 aggagtcgat ccaggggtcg gagtcgcgtt cggaccggta gtactggcgg atggccaccc
     3121 cgatgggccg gtgctcgtcc cggaccaggc cgagggcctg ggcgaagcgg ctggaggcgc
     3181 cgtcggcggc caccaccacc tcggcccgga cgtcgaccgg gtcctcgccc tcgcggcgca
     3241 ccaccgcccc ccggaccaga ccgttctcga ccagcggctc gaccacgtcg gtggcctccc
     3301 acaggtcggc cccggccttg gaagcggttc tggcgaggag ctggtc
//
LOCUS       DASSWR010000150         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_203818,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000150 DASSWR010000000
VERSION     DASSWR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203818"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1055)
                     /locus_tag="VFO47_02475"
     CDS             complement(423..1055)
                     /locus_tag="VFO47_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET8787598.1"
                     /translation="MLTVFSDLHCPWAYLFSIRLRRAREGAGRPPVAWRCWPLELVNE
                     RGTPWATLSQEIPVLTQLEPDHFAPPKRDTWPSTLLPAMEALKVAGELGGATAADRFD
                     EAARRAFFLDNRDLSIRPTLADLATEAGLDRAAFLDAFDGGGHRRAVVADWQEGRRRG
                     VEGSPHAFLPDGSGVFNPGIGKIDWVRGIPVPHDVDEGAITRLLDKATSA"
     gene            1348..>2314
                     /locus_tag="VFO47_02480"
     CDS             1348..>2314
                     /locus_tag="VFO47_02480"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET8787599.1"
                     /translation="MLVLALALVVGWTATPAAALPAPRVEAKTGVVMDAATGAVLWQR
                     QAHRPVLVASTTKILTAIVAADRYPARKVFRVPEAAERVDGTRFGYRTGWLIRRDQLL
                     TTLMLVSANDAAETLAAAWPDGGRAGFLRAMQDKAAELGCTDSTWRDPSGLDVPGHRA
                     SAADLALLGRALLERPVLAKLAGSRVAPYTWPGGRKTVLGNHNHFVGQGRDPGALGVK
                     TGYTTKAGSTIVAAQRRGGRTLVAVVLGSPAMYDDVRALMGYGFKVKPQVGAELLGVR
                     PQPAEEVTTDATLPPAAERVTGRVSGTPSPLVERLGLRLLAAPLPM"
BASE COUNT          284 a          860 c          869 g          301 t
ORIGIN      
        1 ctcacggcag gccaggctct gggcgggctg gttgacgaac accatgaacc cgccgatccc
       61 gagggccacg accagcagga ggcaggccgc cgccagcagc gcagtcggac gccgccggcg
      121 ccgctgggcc aggtgcctgc cgccaaccgt tgctcgctcg ttctccatat gaattcggcc
      181 acttctggcc ggtcggcgcg accgggggtc gtccgcgcct cggggagccg gaccgacctg
      241 gctccaccgt gtccgcagag attgccgcgt tcgctgccag tgtgcaaaat ccggcgccga
      301 tcaggcgggt tcgtcgcatt tctccgggga tcgttcgacg ggagcgactg gttggagcct
      361 gtcgtccggt cagggcgccc gtggtcagcc attggtgccc cgctcctcgg cctcggccag
      421 ggtcaggccg aggtggcctt gtcgaggagg cgggtgatgg cgccctcgtc gacgtcgtgg
      481 gggacgggga tgccgcggac ccagtcgatc ttgccgatgc cggggttgaa gacgccgctg
      541 ccgtcgggca ggaaggcgtg gggggagccc tcgacgccgc ggcggcggcc ctcctgccag
      601 tcggcgacca cggcgcggcg gtggccgccg ccgtcgaagg cgtcgaggaa cgcggcccgg
      661 tccaggccgg cctcggtggc caggtcggcc agggtgggcc ggatcgacag gtcgcggttg
      721 tcgaggaaga aggcccggcg ggcggcctcg tcgaagcggt cggccgccgt ggccccgccc
      781 agctcgccgg cgaccttcag ggcctccatg gccggcagca gggtcgaggg ccaggtgtcg
      841 cgcttcggcg gcgcgaagtg gtccggctcc agctgggtga gcaccgggat ctcctggctc
      901 agggtggccc acggggtgcc gcgctcgttg acgagctcca gcggccagca gcgccaggcc
      961 accggcggcc ggccggcccc ctcgcgggcc cggcgcaggc ggatggagaa caggtacgcc
     1021 caggggcagt ggaggtcgga gaagacggtc agcacgcgcg gctcctaacg gggggacctg
     1081 gacaatcaag ctcatgggcg aacctggcga ggttgtgtca cccttgaaga tatcgccgca
     1141 gggtgccgcg gaaaccggtc gaagggccgg cgccccggtg ccctcgcaac cggagcctcg
     1201 cgccggtcga agggccggcg tcgagctccc ggcgatcacc caggcggcgg cgccccccgc
     1261 cgcccgccgt ccgggtcgag ggtgttgcac tccttccacg ggttcgtgca gcctaccggc
     1321 gtgcgcaccc gccaacgatg gttgccgttg ctggtcctgg cgctcgccct ggtcgtggga
     1381 tggacggcga cgcccgccgc cgccttgccc gcgccccggg tcgaggcgaa gacgggtgtg
     1441 gtcatggacg ccgccaccgg ggccgtgctg tggcagcgcc aggcccaccg gccggtcctg
     1501 gtcgcctcca cgaccaagat cctcaccgcc atcgtggccg ccgaccgcta cccggcccgg
     1561 aaggtgttcc gggtcccgga ggcggccgag cgggtcgacg gcacccgctt cggctaccgg
     1621 accggctggc tgatccggcg cgaccagctg ctcaccaccc tgatgctcgt ctcggccaac
     1681 gacgccgccg agaccctggc cgcggcctgg ccggacgggg gccgggccgg cttcctccgg
     1741 gccatgcagg acaaggcggc cgagctgggc tgcaccgact cgacctggcg cgacccgtcg
     1801 ggcctggacg tccccggtca ccgggccagc gccgccgacc tggccctcct cggccgggcc
     1861 ctgctggagc ggccggtgct ggccaagctg gccgggtcgc gggtcgcgcc ctacacctgg
     1921 ccgggcggcc gcaagacggt gctcggcaac cacaaccact tcgtcggcca gggccgcgac
     1981 cccggcgccc tcggggtcaa gaccggctac accaccaagg ccggctccac catcgtggcc
     2041 gcccagcggc gcggcggccg caccctggtc gcggtcgtcc tcggctcccc ggccatgtac
     2101 gacgacgtgc gcgccctgat gggctacggg ttcaaggtca agccccaggt cggggccgag
     2161 ctgctcggcg tgcgccccca gccggccgag gaggtcacca ccgacgcgac cctgcccccg
     2221 gccgccgagc gcgtcaccgg gcgggtctcg ggcacgccgt ccccgctggt cgagcgcctc
     2281 ggtctgcgcc ttctggccgc gcccctgccg atgg
//
LOCUS       DASSWR010000151         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_656000,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000151 DASSWR010000000
VERSION     DASSWR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656000"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2542
                     /gene="mfd"
                     /locus_tag="VFO47_02485"
     CDS             <1..>2542
                     /gene="mfd"
                     /locus_tag="VFO47_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET8787600.1"
                     /translation="VAEGLDVEGVESLLPLLFDHLEPLPAYLPGDALLVLVDPKRTLD
                     RAEEVRRQADEASHASWASAAEGATAPVEGVAYRPLEEVLEEAGRALLRLGPFDSGQA
                     SAVRIDAHAVEPYRANMTRVAADARDLVADGATVLLCTEGAGPAQRLVEVLREEGLTV
                     PDLAPELPPEPDPGGPDGTSSRVRIRPGVLVGTAPLLTGFRLPSLRLALVAEGDLYGT
                     RRQTREQARMPSSRRRAKHAGGQLALEELQPGDIVVHAVHGIGRYVGMEHRSVGGAER
                     DYLVLAYDQGDKLYLPSEQVELISRYVGGEHPKLSRLGRREWDRQKARVRKKVREMAA
                     ELVRLYSARMASPGHAFGPDTPWQRELEDAFPFTETPDQLTAIEEIKADMEAPVPMDR
                     LLCGDVGYGKTEVAVRAAFKAVMDGKQVGVLVPTTLLAQQHMAVFSERFAPFPVKVAV
                     LSRFQSRQEQEEIVAGMADGTVDLVIGTHRLLSADAKWSDLGLVVVDEEHRFGVGHKE
                     HLKQLRTEVDVLTLTATPIPRTMEMAISGIRDLSVMETPPEERHPVLTFVGAYDQGTV
                     ANAIRREMLREGQTFFVHNRVDTIDRVAYEVRHAIPEARVAVAHGQMTEDQLERVMLD
                     FWDKQYDVLVCTTIIESGIDVPSANTLIVDRADTLGLAQLYQLRGRVGRSRERAYAYL
                     FYPPERSITETSHQRLATVATHQDLGSGRAIAMKDLEIRGAGNLLGADQSGHVALVGY
                     DMYMQLLAEAVAELRGRPIEAPKELKLEVPVDAHLPAAYVPRERLRLEAYRRLGGARV
                     VEEVEALGAELADRYGPPPPPVRNLLRLAGVRAQANAVGLTEVVCFGGR"
BASE COUNT          352 a          994 c          878 g          318 t
ORIGIN      
        1 ggtcgccgag ggcctcgacg tcgagggggt cgagtcgctg ctgccgctgc tgttcgacca
       61 cctcgagccg ctgccggcct acctgcccgg cgacgccctg ctggtgctgg tcgaccccaa
      121 gcgcaccctc gaccgggccg aggaggtccg ccgccaggcc gacgaggcca gccacgcctc
      181 ctgggcgagc gccgccgagg gcgccaccgc cccggtcgag ggcgtcgcct accggcccct
      241 cgaggaggtc ctggaggagg ccgggcgggc cctgctccgg ctcgggccct tcgactccgg
      301 gcaggccagc gccgtccgga tcgacgccca cgccgtcgaa ccctaccggg ccaacatgac
      361 ccgggtcgcc gccgacgccc gcgacctggt cgccgacggg gccacggtgc tgctctgcac
      421 cgagggcgcc ggcccggccc agcggctggt cgaggtcctg cgcgaggagg gcctgaccgt
      481 ccccgacctg gctcccgagc tgccgcccga gccggacccg ggggggcctg acggaacgag
      541 ctcccgcgtc cggatcagac ccggggtcct ggtcgggacc gcgccgctgc tcaccggctt
      601 ccgcctgccg tcgctacgcc tggccctggt cgccgagggc gacctgtacg gcacccgccg
      661 ccagacccgc gagcaggccc gcatgccctc cagccgccgc cgggccaagc acgccggtgg
      721 ccagctcgct ctggaggagc tccagccggg cgacatcgtc gtccacgccg tccacggcat
      781 cggccgctac gtcggcatgg agcaccgcag cgtcggcggg gccgagcgcg actacctggt
      841 cctcgcctac gaccagggcg acaagctgta cctgcccagc gagcaggtcg agctgatcag
      901 ccgctacgtc ggcggcgagc accccaagct gtcgcggctg ggccgccgcg agtgggaccg
      961 ccagaaggcc cgggtgcgca agaaggtccg cgagatggcg gccgagctgg tccggctcta
     1021 ctcggcccgg atggcctcgc ccggccacgc cttcggcccc gacaccccct ggcagcggga
     1081 gctcgaggac gccttcccgt tcaccgagac ccccgaccag ctcaccgcca tcgaggagat
     1141 caaggccgac atggaggcgc cggtgccgat ggaccggctg ctctgcggcg acgtcggcta
     1201 cggcaagacc gaggtcgcgg tccgggccgc cttcaaggcg gtcatggacg gcaagcaggt
     1261 cggcgtgctg gtgcccacga ccctgctcgc ccagcagcac atggccgtgt tctccgagcg
     1321 gttcgcgccc ttcccggtga aggtggccgt gctgtcgcgc ttccagtccc gccaggagca
     1381 ggaggagatc gtcgccggca tggccgacgg caccgtcgac ctggtcatcg gcacccaccg
     1441 gctgctgtcg gccgacgcca agtggtccga cctgggcctg gtcgtggtcg acgaggagca
     1501 ccgcttcggg gtcggccaca aggagcacct caagcagctc cggaccgagg tcgacgtgct
     1561 caccctcacc gccaccccga tcccccggac catggagatg gccatctccg ggatccgcga
     1621 cctgtcggtg atggagaccc cgcccgagga gcgccacccg gtgctcacct tcgtcggcgc
     1681 ctacgaccag ggcacggtgg ccaacgccat ccgccgcgag atgctccgcg agggccagac
     1741 cttcttcgtc cacaaccggg tcgacaccat cgaccgggtc gcctacgagg tccgccacgc
     1801 catcccggag gccagggtgg ccgtcgccca cggccagatg accgaggatc agctcgagcg
     1861 ggtgatgctc gacttctggg acaagcagta cgacgtcctg gtctgcacca ccatcatcga
     1921 gtccggcatc gacgtgccct cggccaacac cctgatcgtc gaccgggccg acaccctcgg
     1981 cctggcccag ctctaccagc tccgggggcg ggtcgggcgc tcgcgggagc gggcctacgc
     2041 ctacctgttc tacccgcccg agcggtccat caccgagacc tcccaccagc ggctggccac
     2101 cgtggccacc caccaggacc tcggctcggg cagggccatc gccatgaagg acctcgagat
     2161 ccgcggcgcc ggcaacctgc tcggggccga ccagtccggg cacgtcgccc tggtcggcta
     2221 cgacatgtac atgcagctgc tggccgaggc cgtggccgag ctgcgcggcc gcccgatcga
     2281 ggcccccaag gagctgaagc tggaggtccc ggtcgacgcc cacctgcccg ccgcctacgt
     2341 gccgagggag cgcctgcgcc tggaggccta ccgccgcctc ggcggggcca gggtggtcga
     2401 ggaggtcgag gccctcgggg ccgagctggc cgaccgttac ggcccgcccc cgcccccggt
     2461 ccgcaacctc ctgcggctgg ccggcgtccg cgcccaggcg aacgccgtcg gcctcaccga
     2521 ggtcgtctgc ttcggaggcc gg
//
LOCUS       DASSWR010000152         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_458184,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000152 DASSWR010000000
VERSION     DASSWR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458184"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VFO47_02490"
     CDS             complement(<1..1221)
                     /locus_tag="VFO47_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET8787601.1"
                     /translation="MTDQPRLALTFEDVLLVPRRSSIRSRRHVTTRSRFTRGIELAIP
                     IVSANMDTVTTAPMAIAMAELGGLGVLHRFLPVDAQAEEVRRVKRYLGQVIEQPYTIG
                     PERTVAEAAAEARRLGVTGLVVVDPERRPVGLLTARDMRADAGDTVAAAMTPAERLIT
                     ARPGIDLDEARKLLDRHRIEKLPLVGDDGRLAGLITLRDIGLRERLPQATRDGQGRLR
                     VAAALGVRGGYLARAQALLDADVDALVVDVAHGHADHTIDAVKELKATWPDSQVVAGN
                     VATADGFADLAEAGADAVKVGIGPGFACTTRLVAGVGVPQLTAILDCAEVARATGVPL
                     IADGGIRHPGDVAKAIAAGASTVMIGGLFAGRPESPGEVVRRQGREYKVFRGLASRGA
                     AAARLEIEGRGDALD"
     gene            1306..>2030
                     /locus_tag="VFO47_02495"
     CDS             1306..>2030
                     /locus_tag="VFO47_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HET8787602.1"
                     /translation="MNTTAGSDEPRLPPELRRFVEIHRRWTENEGIVPTLDELAGDLG
                     CAKSTASQHRRRLRELGLVQFDPRRRRSTRITTDAAILAVYGLAPVGPTDTKVTNLAR
                     FRSRRARLGGLRASQPLGDLQGERPWRLPDRPPRSLPVPGAIPAGPDRTFYPDEDRME
                     VDAELAGPGQYGLRVRGDSMVALGIYDGDQAVVDTRQPAHHGDLVAALFPSDHGDEDL
                     ASLKILELRDDKAWLVAANSAYAP"
BASE COUNT          275 a          707 c          776 g          272 t
ORIGIN      
        1 gtcgagggcg tcgccgcggc cctcgatctc caggcgggcc gcggcggcgc cccgggaggc
       61 caagccccgg aacaccttgt actcgcggcc ctggcggcgg acgacctcgc ccgggctctc
      121 ggggcggccg gcgaacaggc cgccgatcat caccgtgctc gcgccggcgg cgatcgcctt
      181 ggcgacgtcg ccggggtggc ggatgccgcc gtcggcgatc agggggacgc cggtggccct
      241 ggcgacctcg gcgcagtcga ggatggcggt gagctggggc accccgaccc cggcgaccag
      301 gcgggtggtg caggcgaagc cgggcccgat ccccaccttg acggcgtcgg ccccggcctc
      361 ggccaggtcg gcgaagccgt cggcggtggc gacgttgccg gcgaccacct ggctgtccgg
      421 ccaggtcgcc ttcagctcct tgacggcgtc gatggtgtgg tcggcgtggc cgtgggcgac
      481 gtcgacgacc agggcgtcga cgtcggcgtc gagcagggcc tgggccctgg ccaggtagcc
      541 gccgcgcacg ccgagggcgg ccgcgacccg gagccggccc tggccgtcgc gggtcgcctg
      601 gggcaggcgc tcgcgcaggc cgatgtcgcg cagggtgatc aggccggcca gccggccgtc
      661 gtcgccgacc aggggcagct tctcgatccg gtggcggtcc agcagcttgc gggcctcgtc
      721 caggtcgatg ccggggcggg cggtgatcag ccgctcggcc ggggtcatgg ccgcggccac
      781 ggtgtcgccg gcgtcggcgc gcatgtcgcg ggcggtgagg aggccgaccg ggcggcgctc
      841 ggggtcgacc accaccaggc cggtcacgcc caggcgtctg gcctcggcgg cggcctcggc
      901 cacggtccgc tcggggccga tggtgtaggg ctgctcgatc acctggccca ggtagcgctt
      961 gacccgccgg acctcctcgg cctgggcgtc gacgggcagg aagcggtgga ggacgccgag
     1021 cccgcccagc tcggccatgg cgatggccat gggcgcggtg gtcaccgtgt ccatgttggc
     1081 cgagacgatg gggatggcca gctcgatccc gcgggtgaag cgggagcggg tcgtgacgtg
     1141 gcggcgggaa cggatgctgg agcgccgcgg caccagcagg acgtcctcga aggtcagggc
     1201 gaggcgtggt tggtcggtca tggggcccag gctagccgag cgaacagcgt ggtgctcccc
     1261 ctcgtgtcat tgatggcaga ccgaacatgt gttctaatcg acgtgatgaa cacgactgcg
     1321 ggttctgacg aacccaggct gcctccggag ctccgccgct tcgtcgagat ccatcgtcgg
     1381 tggaccgaga acgaaggaat cgtcccgacc ctcgacgagc tggccggcga cctcggctgt
     1441 gccaagtcaa cggcgagcca gcaccgacgg cggctccgag agctcgggct cgtccagttc
     1501 gacccacgaa gacgcaggag cacacggatc acgaccgacg ccgccatctt ggctgtctat
     1561 gggctcgccc cggtcgggcc aaccgacacc aaggtcacca atctcgcccg gttccggagc
     1621 cggcgcgccc ggctcggcgg tctccgtgcc agccagcccc tgggcgacct ccagggcgag
     1681 cggccgtggc gactccccga tcggccaccg cgaagccttc ccgtgcccgg agcgatccca
     1741 gccggaccgg accgcacctt ctatccggac gaggacagga tggaggtcga cgccgagctc
     1801 gccggtccgg gccaatacgg gctccgggtg cgaggtgaca gcatggtggc gctcggcatc
     1861 tatgacggcg accaggcggt cgtggacacc aggcagccgg cccaccatgg cgatctcgtg
     1921 gcggcgctgt ttccgagcga ccatggtgac gaggaccttg cctctctgaa gatcctcgag
     1981 ctgagggacg acaaggcctg gctggtggcc gcgaactccg cctacgcgcc
//
LOCUS       DASSWR010000153         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1249202, whole genome shotgun sequence.
ACCESSION   DASSWR010000153 DASSWR010000000
VERSION     DASSWR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249202"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFO47_02500"
     CDS             complement(<1..620)
                     /locus_tag="VFO47_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787603.1"
                     /translation="MTTELTVRDVFDLQLREVSGICERAAGDGRPRQLLAIGDDSHHL
                     LVGDLEPAVPASFARHDLEPLLDAAGVGIGRSSQWEGVDTDETGRVFVLREVPGTVFV
                     FDPALGRLLHVLGLTVEDDPDEQRAWEADENALGEGLLLLDNGHLFVVKEEKPRQLLE
                     FGLPGEQAQGLHHDLLITDTGRFPTPEEATATFHMLSAWDLGGEAL"
     gene            756..1238
                     /locus_tag="VFO47_02505"
     CDS             756..1238
                     /locus_tag="VFO47_02505"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET8787604.1"
                     /translation="MTDTPSDPPQYPTQPGPPPGQYPQGQYPQGQYPPGDYQAAPRRR
                     TGMGTAALVLGVVALVLVLLLIFSPLGAFLGLLAVIFGIVGIVRANRREADNRGQAVT
                     GLITGGFALVLGIFFTISVGVWFSTHVNDFNRFGQCLDDATGQAAREECARELSRNLE
                     "
     gene            complement(1250..1864)
                     /locus_tag="VFO47_02510"
     CDS             complement(1250..1864)
                     /locus_tag="VFO47_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET8787605.1"
                     /translation="MDGALELDDWRRRVALLYLTGPTEGEAGVAAFRRGRDELFRDHP
                     QSALDDGQRTRFTGLPWFPYDPGARVTAEPRPPDDGSELAVDTGGEDGTVRYRRVARL
                     ATPFGDLTLFWTLGYGGGLFLPFRDATAGDETYGAGRYLTDTVKGTWGRGLVHQGGRV
                     VLDFNYAYNPSCAYNARWACPLAPPENHLNAAVRAGERAYVDPA"
     gene            complement(1875..>2219)
                     /locus_tag="VFO47_02515"
     CDS             complement(1875..>2219)
                     /locus_tag="VFO47_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET8787606.1"
                     /translation="AFVLELFVPDPTLHPGGQSVRARHLGLDLVRLDVAVHDPAAQRV
                     DFQNVLLTAGGIQVLPGAVRYAWPSELDLMARLAGLRLRERWGGWRREPYTASTKGLY
                     VAVYEHAGRPAS"
BASE COUNT          292 a          826 c          814 g          287 t
ORIGIN      
        1 tccagggcct caccgcccag gtcccaggcg gacagcatgt ggaaggtggc cgtggcctcc
       61 tccggggtcg ggaaccggcc ggtgtcggtg atcagcaggt cgtggtggag gccctgggcc
      121 tgctcgccgg ggagcccgaa ctcgagcagc tggcgcggct tttcctcctt gaccacgaac
      181 aggtggccgt tgtcgagcag cagcagcccc tcgccgaggg cgttctcgtc ggcctcccag
      241 gcccgctgct cgtccgggtc gtcctcgacg gtcaggccga ggacgtggag cagccgcccc
      301 agcgccgggt cgaacacgaa caccgtgccc gggacctcgc ggagcacgaa cacccgcccc
      361 gtctcgtcgg tgtcgacccc ctcccactgg gagctgcggc cgatgcccac gcccgccgcg
      421 tccagcagcg gctccaggtc gtggcgggcg aagctggccg gcaccgccgg ctccaggtcg
      481 cccaccagca ggtggtggct gtcgtcgccg atggccagca gctgacgggg ccgcccgtcg
      541 ccggcggccc gctcgcagat ccccgacacc tcccggagct gaaggtcgaa cacgtccctg
      601 acggtgagct cggtggtcat ggcacctccc cggtcgggcc aaggatgccc ggcgggcggc
      661 ccgcccaacc ggccgcccgt tcccttgagc ggtgttcaag cccggggtgg aggagtagtg
      721 ttcgcgcctt cgagcgacgc ctacaggagg tcgacatgac cgacacccct tcggacccgc
      781 cccagtaccc gacccagccg ggcccgccgc ccggccagta cccgcagggc cagtacccgc
      841 agggccagta cccgcccggc gactaccagg ccgcgccccg gcgacgcacc ggcatgggca
      901 cggccgccct cgtcctcggc gtggtcgcgc tggtgctggt gctgctgctg atcttctcgc
      961 ccctgggcgc gttcctcggc ctgctggcgg tgatcttcgg catcgtcggc atcgtacggg
     1021 ccaaccggcg cgaggccgac aaccgcggcc aggccgtgac cgggctgatc accggcggct
     1081 tcgccctggt gctcgggatc ttcttcacca tcagcgtcgg cgtctggttc tcgacccacg
     1141 tcaacgactt caaccgcttc gggcagtgcc tcgacgacgc caccggccag gcggcccgcg
     1201 aggagtgcgc ccgcgagctg tcgcggaacc tcgagtaagc cggaggagat caggccgggt
     1261 cgacgtaggc ccgctccccg gcccggaccg ccgcgttgag gtggttctcg ggcggggcca
     1321 gcgggcacgc ccagcgggcg ttgtaggcgc acgacgggtt gtaggcgtag ttgaagtcga
     1381 gcaccacccg gccgccctgg tggaccaggc cgcggcccca ggtgcccttg accgtgtcgg
     1441 tcaggtagcg cccggccccg taggtctcgt cgccggcggt ggcgtcccgg aaggggagga
     1501 acaggccgcc gccgtagccg agggtccaga acagggtcag gtcgccgaac ggggtggcca
     1561 ggcgggccac ccgccggtag cggacggtgc cgtcctcgcc gccggtgtcc acggccagct
     1621 cggaaccgtc gtccggcggg cgcggttcgg cggtcacccg ggccccgggg tcgtagggga
     1681 accagggcag gccggtgaac cgggtccgct gcccgtcgtc gagggccgac tgggggtggt
     1741 cccggaacag ctcgtcgcgg ccccgccgga acgccgccac cccggcctcg ccctcggtcg
     1801 ggccggtcag atacagcagc gcgacccgcc gccgccagtc gtccagctcc agcgccccgt
     1861 ccatggaccg catgctacga ggcgggccgg ccggcgtgct cgtagacggc cacgtacagg
     1921 cccttggtgg aggcggtgta cggctcccgg cgccagccgc cccagcgctc gcgcagccgc
     1981 agcccggcca ggcgggccat caggtcgagc tcggacggcc aggcgtagcg gaccgctccc
     2041 ggcagcacct ggatgccgcc ggcggtcagc aggacgttct ggaagtcgac ccgctgggcg
     2101 gccgggtcgt gcacggcgac gtcgaggcgg accaggtcca ggccgaggtg acgggcccgc
     2161 acgctctgac cgccggggtg gagagtcggg tcggggacga acagctcgag cacgaacgc
//
LOCUS       DASSWR010000154         4381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_119194,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000154 DASSWR010000000
VERSION     DASSWR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119194"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFO47_02520"
     CDS             <1..358
                     /locus_tag="VFO47_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787607.1"
                     /translation="ANLAEQVASQADRVGDYLKAASGDRILRDVEDFARRQPMLVAAG
                     ALALGFAASRFLKASSGRRYESAYRSYGDGAYDRTYATSPYDTRTAYGTGTSYGTGAG
                     YETEVPVTDVYAERRT"
     gene            358..789
                     /locus_tag="VFO47_02525"
     CDS             358..789
                     /locus_tag="VFO47_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET8787608.1"
                     /translation="MAATQDPAGTDDLRDRSLGELLKQLSEQTTRLVHQELELAKAEL
                     TEKGKQAGQGAGLFGGAGAIGLAALGALTACFILALDIIMPAWLAALIVAVVYGIVAF
                     VLVKQGQARMKRAAPPLPEQTIETVKEDVEWAKTQMRSDRT"
     gene            807..1307
                     /locus_tag="VFO47_02530"
     CDS             807..1307
                     /locus_tag="VFO47_02530"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787609.1"
                     /translation="MSETVGAVGYKADVPSRAKEAVSDKVENVKSKVSDTATRAKEAV
                     TGATDRAGEATPSRGEIEQQARRTAGLAKENPLGLAIGAAAIGFLAGLAVPSTKVEDE
                     KLGPVADQVKDKVKETGQEALDRGKQVAQEVASTAADTAKEKTQEQGQELAGSARQHA
                     EDVRTQ"
     gene            1304..2275
                     /locus_tag="VFO47_02535"
     CDS             1304..2275
                     /locus_tag="VFO47_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8787610.1"
                     /translation="MTERDNGQAAGRDAERPGEIPPRGWFAILKRVKAEVKEDNVTLL
                     AAGVAFYAMLAIFPAIIAVVTVYGMVADPAQVESQVDEFAKGLPAGAGELITEQLNNV
                     VNAGRQALSIGLAISLLAVLWSASGGVQGLVKSLNLVYDERETRGFVKLRGLSLLLTL
                     GAIVVVVVALALIAVFPGVIDDLGLGQAGELAASISRWVVLALVVLTALAVLYRYAPD
                     RANPRWRWVSWGAVVALVVWLLGSVGFSWYVDNFGKYNQTYGALASVIVLLLWLFLSS
                     FAVLLGAEFDAEIERQTARDTTTGPDRPLGERDAEVADTVGESPRSR"
     gene            2272..3051
                     /locus_tag="VFO47_02540"
     CDS             2272..3051
                     /locus_tag="VFO47_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET8787611.1"
                     /translation="MTAPLIAIPTYHLGPGRVGNWEGAYALPEPYVAALVAAGAQVAL
                     LPPSAPADPEALLAAFDGLLLAGGGDIEPARYGAADHPAQYGTDPDRDELELELARAA
                     VRLGTPTLAICRGVQLLNVAFGGTLVQHLPDGDGLVEHRNEAKQAMHGLRVEPGSRLA
                     EALGQVEAEGLSHHHQSLDRLGDGFRPVAWAPDGLVEGIERDHGWTVGVLWHPESTAQ
                     ADPVQHRLLRSFVEVASRRGQAFWRRRAEPVGFAGPGTTAG"
     gene            3103..3459
                     /locus_tag="VFO47_02545"
     CDS             3103..3459
                     /locus_tag="VFO47_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787612.1"
                     /translation="MAVAIALTVLFPLGLMGLLAAGEWLERRTLATEEVVPRRLRRMD
                     TKPPEAVEAMVLEETAHVVAQYWSATGRPASEAPTPRPATNGDQPATRSRSNGNGGRH
                     LRRPATGRAGRHERRR"
     gene            3544..>4381
                     /locus_tag="VFO47_02550"
     CDS             3544..>4381
                     /locus_tag="VFO47_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787613.1"
                     /translation="MQRRPRTSEAFVAGVAAMTVATAVLGLVHLVQASFPFPPLGLAQ
                     RLLRLVPGPVAVFFIDLLGHWALRLFAVGFTVGSILAGGGAAVLVARTAGPGAHVAED
                     GSPRRTAPSRPARAAWLAAGALGLAALAGYRVQPGAPSLLVYGVVVAVAAAVYASFLL
                     GALGRLERDPVPAPASGLGRTRRELLRAVVGAAGLLATGFAIRRLTGGIGDSGGQPLA
                     RPAGAAPVRPAPAPAAGDDQATWAIAGLTPEVTSNRLHYTVDESIIDPNVDSGSWRLR
                     VAG"
BASE COUNT          584 a         1628 c         1613 g          556 t
ORIGIN      
        1 ggcgaacctg gccgagcagg tcgccagcca ggccgaccgc gtgggcgact acctcaaggc
       61 ggccagcggc gaccggatcc tgcgcgacgt cgaggacttc gcccgccgcc agcccatgct
      121 ggtcgcggcc ggcgccctcg ctctcggctt cgccgcctcc cggttcctga aggcgtccag
      181 cggccgccgc tacgagtccg cctaccggag ctacggcgac ggggcctacg accggaccta
      241 cgccaccagc ccctatgaca cccggacggc ctatggcacc gggacgtcgt acggcaccgg
      301 cgccggctac gagaccgagg tgcccgtcac cgacgtctac gccgagcgga ggacgtgatg
      361 gccgcgaccc aggacccggc gggcaccgac gacctgcgcg accgttccct gggtgagctg
      421 ctcaagcagc tctcggagca gaccacccgg ctggtccatc aggagctgga gctggccaag
      481 gccgagctga ccgagaaggg caagcaggcc ggccagggcg ccgggctgtt cggcggcgcc
      541 ggggccatcg gcctggccgc cctcggggcg ctgacggcct gcttcatcct cgccctggac
      601 atcatcatgc cggcctggct ggccgccctg atcgtggccg tcgtctacgg gatcgtggcc
      661 ttcgtcctgg tcaagcaggg ccaggccagg atgaaacggg cggccccgcc gttgcccgag
      721 cagaccatcg agaccgtgaa ggaggacgtg gaatgggcca agacccagat gcgatccgac
      781 aggacataga gcagacccga gccgagatga gcgagaccgt cggggccgtc ggctacaagg
      841 cggacgtgcc ctcgcgggcc aaggaagcgg tgagcgacaa agtggagaac gtcaagtcga
      901 aggtgagcga caccgccacc cgggccaagg aggccgtgac cggcgcgacc gaccgggccg
      961 gcgaggccac cccgagccgc ggggagatcg agcagcaggc ccggcggacg gccgggctgg
     1021 ccaaggagaa cccgctgggg ctggccatcg gcgccgccgc catcgggttc ctggccgggc
     1081 tggccgtgcc ctcgaccaag gtcgaggacg agaagctggg cccggtggcc gaccaggtca
     1141 aggacaaggt caaggagacc ggccaggagg cccttgaccg gggcaagcag gtcgcccagg
     1201 aggtggccag caccgccgcc gacaccgcca aggagaagac ccaggagcag gggcaggagc
     1261 tggccggcag cgcccggcag catgccgagg acgtcaggac gcagtgaccg agcgcgacaa
     1321 cggccaggcg gccggcaggg acgccgagcg ccccggcgag atcccgccga ggggatggtt
     1381 cgccatcctc aagcgggtca aggccgaggt gaaggaggac aacgtcaccc tgctggccgc
     1441 cggcgtcgcc ttctacgcga tgctggccat cttcccggcg atcatcgccg tggtcaccgt
     1501 ctacggcatg gtcgccgacc cggcccaggt cgagtcccag gtggacgagt tcgccaaggg
     1561 cctgccggcg ggcgccggcg agctgatcac cgagcagctc aacaacgtgg tcaacgccgg
     1621 ccggcaggcc ctctcgatcg gcctggcgat cagcctcctg gccgtcctct ggagcgcctc
     1681 cggcggagtc caggggctgg tcaagagcct caacctggtc tacgacgagc gcgagacgcg
     1741 cgggttcgtc aagctgcgcg gcctgtcgct gctgctcacc ctgggggcga tcgtggtcgt
     1801 ggtggtcgcc ctggccctga tcgcggtctt ccccggcgtg atcgacgatc tcgggcttgg
     1861 gcaggccggc gagctggccg cgtcgatctc ccgctgggtg gtgctggccc tggtcgtcct
     1921 gaccgccctg gcggtgctgt accgctacgc ccccgaccgg gccaacccgc gctggcgctg
     1981 ggtcagctgg ggggcggtgg tcgccttggt ggtctggctg ctaggctccg tcgggttcag
     2041 ttggtacgtc gacaacttcg gcaagtacaa ccagacctac ggcgccctgg cctcggtgat
     2101 cgtcttgctc ctctggctgt tcctctcctc cttcgcggtg ctcctcggcg ccgagttcga
     2161 cgccgagatc gagcgccaga cggccaggga caccaccacg ggcccggacc ggccgctggg
     2221 cgaacgcgac gccgaggtcg ccgacaccgt cggtgagagc ccccggagcc ggtgaccgcc
     2281 ccgctgatcg cgatcccgac ctaccatctc ggccccggcc gggtggggaa ctgggagggc
     2341 gcctacgccc tgcccgagcc gtacgtggcc gccctggtcg cggccggcgc ccaggtcgcg
     2401 ctgctgccgc cgtcggcgcc ggccgacccc gaggcgctgc tggccgcctt cgacgggctg
     2461 ctgctggccg gcggcggcga catcgagccg gcccgctacg gtgccgccga ccatccggcc
     2521 cagtacggga ccgaccccga ccgtgacgag ctggagctgg agctggcccg ggcggcggtc
     2581 cggctgggca ccccgaccct ggccatctgc cggggggtcc agctgctcaa cgtggccttc
     2641 ggcggcaccc tggtccagca cctgccggac ggcgacggcc tggtcgagca ccgcaacgag
     2701 gccaagcagg cgatgcacgg cctccgggtc gagcccggca gccgcctggc cgaggcgctc
     2761 ggccaggtcg aggccgaggg cctctcccac caccaccaga gcctcgaccg cctcggcgac
     2821 ggattccggc cggtcgcctg ggcgccggac gggctggtcg aggggatcga gcgcgaccac
     2881 ggctggaccg tcggcgtgct ctggcatccc gagagcacgg cacaggccga cccggtccag
     2941 caccggctgc tgcggtcctt cgtggaggtg gcgtcccggc gtggacaggc gttttggcgg
     3001 cgacgcgccg agccggtagg gtttgccggt cccgggacca ccgcagggtg aaccgggacg
     3061 gccggtccgt gcgtactcca cggtgagatg ctgttctcca acttggctgt cgcgatcgcc
     3121 ctcacggtgc tgttcccgtt ggggctgatg gggctgctgg cggccgggga gtggctggag
     3181 cgccgcaccc tggccaccga ggaggtggtg ccgcggcggc tgcgccgcat ggacaccaag
     3241 ccgcccgagg cggtcgaggc catggtgctg gaggagacgg cccacgtggt cgcccagtac
     3301 tggtcggcca cgggccgccc ggcctccgag gcccccaccc cccggcccgc caccaacggc
     3361 gaccagccgg ccacccgctc ccggagcaac ggcaacggcg gccgccacct gcgccgccca
     3421 gccacgggtc gtgccggccg ccacgagcgc cgccggtagc tttagcgtcc gagcccgacc
     3481 acctgctccg gcgatcggcc gctggaaccc gcccaggacc cttgcggcgg ctatcgtgga
     3541 aggatgcaac ggcgcccgcg cacctccgag gccttcgtcg ccggcgtggc cgccatgacc
     3601 gtggccacgg ccgtcctcgg gctcgtccac ctggtccagg ccagcttccc gttcccgccc
     3661 ctggggctcg cccagcggtt gctgcggctg gtccccgggc cggtggcggt gttcttcatc
     3721 gacctgctgg gccactgggc cctgcggctg ttcgcggtcg gcttcaccgt cggcagcatc
     3781 ctggccggcg ggggagcggc cgtgctggtg gccaggacgg cgggtccggg ggctcatgtg
     3841 gccgaagacg gcagcccccg gagaacagct ccgtcccgcc cggccagggc ggcctggctg
     3901 gccgcgggcg cgctcgggct ggccgccctg gccggctacc gggtccagcc gggcgcgccc
     3961 tcgctgcttg tctacggtgt ggtggtggcc gtcgcggcgg ccgtctacgc cagcttcctg
     4021 ctcggcgccc tcggccgcct ggagcgcgac ccggtcccgg ccccggccag cgggctcggg
     4081 cggaccaggc gggagctgct ccgggccgtc gtcggggcgg ccgggctgct ggccaccggg
     4141 ttcgccatcc gccggctcac cggcgggatc ggcgacagcg gcggccagcc cctggcccgg
     4201 ccggccggcg ccgccccggt ccgcccggca ccggccccgg cggccggcga cgaccaggcc
     4261 acctgggcca tcgccggcct cacccccgag gtcaccagca accggctcca ctacaccgtc
     4321 gacgagtcga tcatcgaccc caacgtcgac agcggctcct ggcggctgcg ggtcgcgggt
     4381 c
//
LOCUS       DASSWR010000155         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_882143,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000155 DASSWR010000000
VERSION     DASSWR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882143"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VFO47_02555"
     CDS             <1..951
                     /locus_tag="VFO47_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8787614.1"
                     /translation="SVARTDLAGPELTEPLARAAYRSLHQRLLPLPDDLGVSPTHGAG
                     SFCSAQVGGERTTTIGAERRHNRLLAASDEDAFVTELLASFGSYPPYFLRLRERNRVG
                     PELLGRDWKVLPLLSTDQAQQHLAGGGLVIDARPITEFAAGHIPGALSIALRPAFASW
                     LGWLVDDTQPLVFVLDDDQDRGELARQCRTIGYDHLVGELAGGMAVWRIANLPQAQLP
                     VVEAEQLGDQPGVVLDVRQASEVADGHLPGAVAVELGALAGADHPELPEGPVTVMCAH
                     GERAMTAASLLARAGHKDLRVVRGGPGDWQRATGQALAHP"
     gene            948..>2155
                     /locus_tag="VFO47_02560"
     CDS             948..>2155
                     /locus_tag="VFO47_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787615.1"
                     /translation="MTQPPPVRPGGLRLGLRANLAQFSLLVGVNALVGGMVGQERSLL
                     SLLATEVFGLAAVSAALTYIVAFGLTKAATNFLAGALADRYGRKPVLVAGWLVGLPVP
                     LLIIWAPTWEWIVAANVLLGVNQGLCWSTTVIMKIDLAGPRRRGLAMGLNEAAGYLAV
                     AVTAYLTGLIAAHAGLRPEPFFLGLAYAGLGLGLSALLVRETRGHAQLEAQLPDAARP
                     TVANPGPNRTLGAAGVERPSTREVFVRTSLREPALSACSQAGLVNNLNDGLAWGLLPL
                     LFVRGGLPVAQVGLLAALYPAVWGLGQLLTGPLSDRLGRKPLITTGMLVQAVALAGTA
                     LAGSFTLWATMAILLGIGTAMVYPTLLAAISDVAHPSWRASAVGVYRLWRDTGFAVGA
                     LLAGLIADLI"
BASE COUNT          249 a          856 c          725 g          325 t
ORIGIN      
        1 tcggtggccc gcaccgacct ggccgggccg gagctgaccg agccgctggc ccgggccgcc
       61 taccggtcgc tgcaccagcg gctcctcccg ttaccggacg acctgggcgt gtccccgacc
      121 catggagctg gttcgttctg ctcggcccag gtcgggggcg agcggaccac caccatcggc
      181 gccgaacgcc gccacaaccg gctgctggcc gcctctgacg aggacgcctt cgtaacagag
      241 ctgctggcca gtttcggtag ctacccgccg tatttcctgc ggctgcggga gcgcaaccgg
      301 gtcggccccg agctgctcgg ccgcgactgg aaggttctgc ccctgctctc gaccgaccag
      361 gcccaacagc acctggccgg cggcgggctg gtgatcgacg cccgccccat caccgagttc
      421 gcggccggtc acatccccgg ggcgctctcg atcgcgctgc gcccggcgtt cgccagctgg
      481 ctaggctggc tggtcgacga cacccagccg ctggtcttcg tgctggacga cgaccaggac
      541 cggggcgagt tggcccgcca gtgccgcacc atcggctatg accacctggt gggtgagctc
      601 gccggcggga tggccgtctg gcgcatcgcc aacctgcccc aggcccagct ccccgtggtc
      661 gaggccgaac agctcggcga ccagcccggc gtggtcctgg acgtccgcca ggccagcgag
      721 gtcgccgacg gccacctccc cggcgcggtc gccgtcgagc tgggcgccct ggccggcgcg
      781 gaccatccgg agctcccgga ggggccggtc acggtgatgt gcgcccacgg cgagcgggcc
      841 atgaccgcgg ccagcctcct cgcccgcgcc ggccacaagg acctgcgggt ggtccgcggc
      901 ggccccggcg actggcagcg ggccaccggc caggccctgg cccacccgtg acccagccgc
      961 caccggtccg ccccgggggg ctgcggctgg ggttgcgggc caacctggcc cagttctccc
     1021 tgctggtcgg ggtcaacgcg ttggtcggtg gcatggtcgg ccaggagcgg agcctgctgt
     1081 cgctgctcgc caccgaggtc ttcgggctgg ccgcggtgtc ggccgctctc acctacatcg
     1141 tcgccttcgg cctcaccaag gcggccacca acttcctggc cggcgccctg gccgaccgct
     1201 acggccgcaa accggttctg gtcgccggct ggctggtcgg cctgccggtg ccgttgctga
     1261 tcatctgggc cccgacctgg gagtggatcg tggccgccaa cgtcctactt ggggtcaatc
     1321 agggcctgtg ctggtcgacc acggtgatca tgaagatcga cctggccggc ccgcgtcgcc
     1381 gcgggctggc catgggcctg aacgaggccg ccggctacct cgccgtcgcc gtcaccgcct
     1441 acctgaccgg cctgatcgcc gcccacgccg ggctgcgccc cgaaccgttc ttcctcggcc
     1501 tggcctacgc cggcctcggc ctcgggttgt cagcgctgct ggtccgcgag acccgcggcc
     1561 acgcccaact cgaggcccag ctgcccgatg ccgcccgccc gaccgtcgcc aaccccggcc
     1621 ccaaccgaac gctgggtgca gccggcgttg aacggccctc cacccgcgaa gtctttgtcc
     1681 gcaccagcct ccgcgagcca gccctgtcgg cctgcagcca ggccgggctg gtcaacaacc
     1741 tcaacgacgg gctcgcctgg ggactgttgc cgctgctgtt cgtccgcggg ggcctccctg
     1801 tcgctcaggt cggcctgctc gccgccctct accccgctgt ttggggcctc ggtcagctcc
     1861 tcaccggccc gctgtcggat cggctgggcc gcaagcccct catcaccaca gggatgctgg
     1921 tccaggcggt tgcgctggct ggcaccgccc tggccggcag cttcacgctc tgggcaacca
     1981 tggccatcct gctcgggatc ggcaccgcca tggtctaccc gaccctgctg gccgccatca
     2041 gcgacgtcgc ccaccccagc tggcgggcct ccgcggttgg cgtctaccgg ctctggcgcg
     2101 acaccggctt cgccgtcggc gccctgctcg ccggcctgat cgccgacctc atcag
//
LOCUS       DASSWR010000156         4728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_571318,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000156 DASSWR010000000
VERSION     DASSWR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571318"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..834)
                     /locus_tag="VFO47_02565"
     CDS             complement(55..834)
                     /locus_tag="VFO47_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8787616.1"
                     /translation="MTSLVRVEVAGRVATVTLDRPEALNAISTELAVELAEAVEPLAT
                     DPGVRAVVLTGAGKRAFCVGADLKQRAGFDDHGWFVQREAFRRGFAAVRRCPLPTVAA
                     VSGFALGGGTELALACDLVVADDDATFGLPEVRLGLVPAGGGTQLLVRRVGRSVAKDL
                     VLTGRRVPAPEAHRLGLVDRVVARGEVLTAATALAAEIAANAPTAVRMAKWAIDLGAD
                     LAQEAAMEVEDEAWRRAVLSDDRREGIAAWGEKREPNWPGG"
     gene            complement(839..1795)
                     /locus_tag="VFO47_02570"
     CDS             complement(839..1795)
                     /locus_tag="VFO47_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8787617.1"
                     /translation="MRTLVTGGSGFIGSALVDRLAGAGHQVIVVDDLSTGRRENLADA
                     FTSGRVELAQIDLAGPELAGVVAGARPEVVYHLAAQIDVRRSVADPLHDARVNVLGTI
                     ALAKAALEAGCRRLVFASSGGTVYGEPDPAALPIDERYPPRVTNPYGVSKRSAEDYLA
                     SLADLHGLEPVSLRLGNVYGPRQDPHGEAGVVAIFCNRLLAGEPVTIFGDGRQTRDYV
                     FVEDVVDAFVAGGQHPDAPGARVNIGTGVETSVLELYDALRGVAGFGAEPEFAPARPG
                     ELARIALDSAEAGRVLGWRSRVGLADGLARTWAWASQEVAPW"
     gene            1915..3447
                     /locus_tag="VFO47_02575"
     CDS             1915..3447
                     /locus_tag="VFO47_02575"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET8787618.1"
                     /translation="MQEAATPVTAGTRAAAASRGWLLPYLMIAVVAVVELLTGRRVIV
                     GGFLVLAPLLASRLSTRRSQVIAVGVVTLATAAALGVFTGKLAGDQLASRVVLITLAT
                     LFALVNWGASQREQSALERAADTIRMAGSLAVGLEPEEAYDLLARSARTLYAANAAAV
                     YRRQGDQMVTVAQARDPDVPAMPLRLPRASFPAAFMTVTRRVSARSTEPEAPMLAARG
                     LASLLWLPLLDRTGDQLGTIVLAWKRDPRLSAQALEASEAFAGLGARAISGSERVRAQ
                     TEVLEQVQALLLTTPPAWVAGFQVGVRYQSASGLAQIGGDFYDVVELDERGLAFILAD
                     ARGKGLEASSLAAVLKGAFRSLAGEGAGPARILNRLDRLVAREGGDEDFVTALAGRLH
                     PDGRILLASAGHPAPLGAGPRLVQVAAPLGLGTQANESQGHLRPGHRLVCYTDGLVEA
                     RNAAGEFLDRSVFEEAVAADSLAAALDRLVELVDRHAAGRHDDDLALLGLEYDPRGGG
                     GG"
     gene            3505..4593
                     /locus_tag="VFO47_02580"
     CDS             3505..4593
                     /locus_tag="VFO47_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8787619.1"
                     /translation="MDVELAPEQEEFRKIVRAFAEQVVAPRAEAMDRAGALDPVVLDQ
                     MAGMGLFGLPFPEEVGGMGADYTTLCLAIEELARVDSSVAITLEAAVGLGAMPIWRYG
                     TAAQHDQWLRPLVTGEKLGAFGLTEPGGGSDAAALTTRASLDGDDWVIDGTKALITNA
                     GLDRSSLVTIAAVTGQRPDGRNELTNIIVPAGTPGYRVGRSYAKVGWHASDTRELIFE
                     SCRVPVANTLGERGSGLANFLRTLEEGRIAVAALATGLCQGCVDESTKYARERQAFGQ
                     PIAGFQAIAFKIADMEVRAHTARLAWQRAAARLDAGLPFGREAAIAKLYASEAAVTSA
                     REAVQVHGGYGFMEEFPVARFYRDAKVL"
BASE COUNT          594 a         1831 c         1722 g          581 t
ORIGIN      
        1 gaagcccgca gcgacgccag ccgctagaag tcgggggggc ccgggggctc aagcctagcc
       61 ccccggccaa ttgggctcgc gcttctcgcc ccaggcggcg atgccctcgc ggcggtcgtc
      121 ggagaggacg gcccgccgcc aggcctcgtc ctcgacctcc atggccgcct cctgggccag
      181 gtcggcgccc aggtcgatgg cccacttggc catgcggacg gcggtcgggg cgttggccgc
      241 gatctcggcc gccagggcgg tggccgcggt cagcacctct ccccgggcca cgacccggtc
      301 gaccaggccg agccggtggg cctcgggggc ggggacgcgg cggccggtga ggaccaggtc
      361 cttggccacc gaccggccca cccgccggac cagcagctgg gtgccgcccc cggccgggac
      421 caggccgagg cggacctcgg ggagcccgaa ggtggcgtcg tcgtcggcca ccaccaggtc
      481 gcaggccagg gccagctcgg tgccgccccc gagggcgaag ccggacacgg ccgccaccgt
      541 gggcaggggg cagcggcgga cggcggcgaa cccgcggcgg aacgcctccc gctgcacgaa
      601 ccagccgtgg tcgtcgaacc cggcccgctg cttgaggtcg gccccgacgc agaaggcgcg
      661 cttcccggcc ccggtcagga ccaccgcccg caccccgggg tcggtggcca gcggctcgac
      721 ggcctcggcc agctcgacgg ccagctcggt cgagatcgcg ttcagcgcct ccggccggtc
      781 gagggtgacg gtggccaccc gcccggcaac ctccacccgg accagcgagg tcattggttc
      841 accagggggc gacctcctgg gacgcccacg cccaggtgcg ggcgagcccg tcggccaggc
      901 cgacccggga ccgccagccg aggacccggc cggcctcggc cgagtcgagc gcgatccggg
      961 ccagctcgcc cggccgggcc ggggcgaact ccggctcggc cccgaacccg gcgaccccgc
     1021 gcagggcgtc gtacagctcc agcaccgacg tctcgacccc ggtgccgatg ttgacccggg
     1081 ccccgggggc gtccgggtgc tgcccgccgg cgacgaaggc gtcgaccacg tcctccacga
     1141 acacatagtc gcgggtctgg cggccgtcgc cgaagatggt caccggctcg ccggccagca
     1201 gccggttgca gaagatggcc accaccccgg cctcgccgtg ggggtcctgg cggggcccgt
     1261 agacgttccc cagccgcagg ctgaccggct ccagcccgtg caggtcggca agtgaggcca
     1321 ggtagtcctc ggccgaccgc ttcgacaccc cgtaggggtt cgtcacccgc ggcgggtagc
     1381 gctcgtcgat cggcagggcc gccgggtcgg gctcgccgta caccgtcccg ccggagctgg
     1441 cgaagaccag ccgccggcag ccggcctcca gggccgcctt ggccagggcg atggtgccga
     1501 gcacgttgac ccgggcgtcg tgcagcgggt cggcgacgct gcggcgcacg tcgatctggg
     1561 cggccaggtg gtagacgacc tcgggccgcg ccccggcgac aaccccggcc agctccgggc
     1621 cggccaggtc gatctgggcc agctcgaccc gtcccgaggt gaacgcgtcg gccaggttct
     1681 cccgccgccc cgtggacagg tcgtcgacca cgatcacctg gtgccctgcg ccggccaggc
     1741 ggtccaccag cgccgacccg atgaaaccgg aaccgcccgt gaccagcgtt cgcatcccag
     1801 ggtcgccttt cgtcgaggtc gggcaaggcg gtcatcgtag aggaagctcc gtcttgtcgt
     1861 cggccactcg atcgttcagg ctccgtcggg caggccaggt gggaggagaa gcacgtgcag
     1921 gaggcggcca cgccggtgac ggccggcacc cgggcggccg cggcgagccg cggctggctg
     1981 ctcccgtacc tgatgatcgc cgtcgtcgcc gtcgtcgagt tgctgaccgg tcgccgggtg
     2041 atcgttggcg ggttcctggt cctggccccg ctactggcca gccggttgtc gaccaggcgc
     2101 agccaggtca tcgccgtcgg cgtggtcacc ctggccacgg ccgccgccct cggggtcttc
     2161 accgggaagc tggccggcga ccagctcgcc agccgcgtgg tcctgatcac cctggccacc
     2221 ctgttcgcgc tggtcaactg gggcgcctcc cagcgcgagc agtcggccct ggagcgggcc
     2281 gccgacacca tccggatggc cgggtcgctg gccgtcggcc tggagccgga ggaggcctac
     2341 gacctgctcg cccggtcggc ccggacgctg tacgcggcca acgcggccgc ggtctaccgc
     2401 cgccagggcg accagatggt gaccgtcgcc caggcccgcg acccggacgt gccggcgatg
     2461 ccgctgcggc tgccgcgggc gagcttcccg gccgccttca tgaccgtgac ccggcgggtc
     2521 agcgcccgct cgaccgagcc ggaggctccg atgctggccg cccgcggcct ggccagcctg
     2581 ctctggctgc cgctgctgga ccgcaccggt gaccagctcg gcaccatcgt gctcgcctgg
     2641 aagcgcgacc ccaggctgtc cgcccaggcc ctggaggctt cggaggcgtt cgccggcctc
     2701 ggcgcccggg ccatctccgg ctccgagcgg gtgcgggccc agaccgaggt gctggaacag
     2761 gtccaggcgc tgctgctcac caccccgccg gcctgggtcg ccgggttcca ggtcggggtc
     2821 cgctaccagt cggccagcgg cctggcccag atcggcggcg acttctacga cgtggtcgag
     2881 ctggacgagc gcgggctggc cttcatcctg gccgacgccc gcggcaaggg gctggaggcg
     2941 agctcgctgg ccgccgtgct caagggcgcc ttccgcagcc tggccggcga gggggccggc
     3001 ccggcccgca tcctcaaccg cctcgaccgg ctggtggccc gcgagggcgg cgacgaggac
     3061 ttcgtgaccg ccctggccgg gcggctccac cccgacggcc gcatcctgct cgcctcggcc
     3121 ggccacccgg ccccgctggg cgccggtccc cggctggtcc aggtggcggc gccgctcggc
     3181 ctcggcaccc aggccaacga gagccagggc cacctgcgtc ccggccaccg gctggtctgc
     3241 tacaccgacg ggctggtcga ggcccgcaac gccgccggcg agttcctcga ccggtcggtg
     3301 ttcgaggagg cggtcgccgc cgactccctg gccgccgccc tcgaccgcct ggtcgagctg
     3361 gtcgaccggc acgccgccgg ccgccacgac gacgacctgg ccctcctcgg cctggagtac
     3421 gacccccggg gtggcggcgg cggttagcat ggccgggcgc ccacaaggcg ccgaccacgc
     3481 tgctcatccc acaagggagt gacgatggac gttgagctgg cacccgagca ggaggagttc
     3541 cgcaagatcg tccgggcgtt cgccgagcag gtggtggccc cccgggcgga ggcgatggac
     3601 cgggccgggg cgctcgaccc ggtggtgctc gaccagatgg ccggcatggg cctgttcggg
     3661 ctgccgttcc ccgaggaggt cggcggcatg ggcgccgact acaccaccct gtgcctggcc
     3721 atcgaggagc tggcccgggt cgactcctcg gtggccatca ccctggaggc cgccgtcggc
     3781 ctcggggcca tgccgatctg gcgctacggc accgccgccc agcacgacca gtggctgcgg
     3841 ccgctggtca cgggggagaa gctgggcgcg ttcgggctca ccgagccggg cggcggctcc
     3901 gacgccgcgg ccctcacgac ccgggccagc ctggacggcg acgactgggt gatcgacggc
     3961 accaaggcgc tcatcaccaa cgccggcctg gaccgctcca gcctggtcac catcgccgcc
     4021 gtgaccgggc agcggcccga cggccgcaac gagctcacca acatcatcgt gcccgccggc
     4081 acccccggct accgggtcgg gcgcagctac gccaaggtcg gctggcatgc ctccgacacc
     4141 cgcgagctga tcttcgagag ctgccgggtc ccggtggcca acaccctcgg cgagcgcggc
     4201 agcggcctgg ccaacttcct ccgcaccctt gaggagggcc gcatcgccgt ggccgccctg
     4261 gccaccggcc tctgccaggg ctgcgtcgac gagtccacca agtacgcccg cgagcgccag
     4321 gccttcggcc agccgatcgc cgggttccag gcgatcgcct tcaagatcgc cgacatggag
     4381 gtccgggccc acaccgcccg cctggcctgg cagcgggccg cggcccggct cgacgccggc
     4441 ctgccgttcg gccgggaggc ggccatcgcc aagctgtacg cctccgaggc ggccgtcacc
     4501 tcggcccgcg aggccgtcca ggtccacggc ggctacggct tcatggagga gttcccggtg
     4561 gcccgcttct accgcgacgc caaggtgctg tagatcggcg agggcaccag cgaggtcatg
     4621 cgcatcctca tcgcccgtgc ggtcgggctc tgacaggagc agcagccgtg ttcgacaagg
     4681 agttcttcgc cacctccctg cccgaggcca tcgccaccta caccgccg
//
LOCUS       DASSWR010000157         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1249402, whole genome shotgun sequence.
ACCESSION   DASSWR010000157 DASSWR010000000
VERSION     DASSWR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249402"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFO47_02585"
     CDS             <1..592
                     /locus_tag="VFO47_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8787620.1"
                     /translation="EDATQEAFLAALRKAASFRRAAAFSTWLYRIAVNAATDQARRRG
                     RARLAALDPEDAGLAVAPGSELGEVVASAVAVQTALTQVPEEFRVAVVLCDLYRVPYA
                     DAAQILEVPVGTVKSRVFRGRLALAECLGAPPDADPGHPPLHVAGPPGTGTAPAASEP
                     TAPDPTRSSSSVPNLHDHQRQGQPRLPDSHDHERQG"
     gene            600..1490
                     /locus_tag="VFO47_02590"
     CDS             600..1490
                     /locus_tag="VFO47_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET8787621.1"
                     /translation="MPDRPDHPDREQLAAFQAGDGERRQRSEVEAHLAGCPSCAEVVA
                     SVARARSGLALLEEPILPAGLHDRVAAAVEAEAAQAAADLPAARRPEASPEVLRPTPW
                     YRRPVAWGAAAALLLAALVVPFLDQSGTMTSADRDAAGTASQEAATEGGGGAAPLPVI
                     RIPGEVTAAKVQATLTVDRRAKTALDAAGGGTPADQAAPPSGSLSREFGQQEAARSST
                     PAPQAHTALLASCLPAATGDADAATRPLTPAFFVEGTYQGREATILVTTSASPPGRVD
                     LWVFPRNDCSAPPVATERVR"
     gene            complement(1811..2302)
                     /locus_tag="VFO47_02595"
     CDS             complement(1811..2302)
                     /locus_tag="VFO47_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787622.1"
                     /translation="MTADQTNLAPTCVNHPKVETRLTCSNCGDPICTRCMVATAVGQK
                     CPRCAKQTGRAKGNPEPQLLARGFGAGVAVAAFGGIVLAAVPFAGLLLALAHGFVVGA
                     AVRWAARRRTHPWLGLAAAAAVMVGLGGLALLLGANPFAGRLLLAYLLSGGVAFARAS
                     GAW"
     gene            2436..3407
                     /gene="trxB"
                     /locus_tag="VFO47_02600"
     CDS             2436..3407
                     /gene="trxB"
                     /locus_tag="VFO47_02600"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET8787623.1"
                     /translation="MATPRTTAPVLIVGSGPAGLTAALYTARANLHPVVLEGLEAGGQ
                     LMLTTEVENYPGFPTGILGPELMGKMREQAERFGAEFVRADATKVDLAGPPFRAWVED
                     QEYSGKALIIATGAKAKMLGLPGESQYLGRGVSTCATCDGFFYRNRELVVVGGGDSAL
                     EEATFLTRFATKVTVVHRRDRLRASKVMQDRAFANPKIAFEWNSVVVDVLGEQTVTGV
                     RLRNVEDGSEKELPVAGVFVAIGHTPNTDLFKSQVEFDEAGYIVTPDPPSTATSVDGV
                     FAAGDVVDHTYRQAVTAAGTGCAAAIDAERWLEAHGHDSGATTDNWD"
     gene            3541..>3798
                     /gene="trxA"
                     /locus_tag="VFO47_02605"
     CDS             3541..>3798
                     /gene="trxA"
                     /locus_tag="VFO47_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET8787624.1"
                     /translation="MTKAAAVTDATFDNEVLKSSTPVLVDFWAEWCGPCKMISPLLEE
                     IADEHKGKLTIAKLNVDENPDVAMRYGVMSIPTLALFIGGVE"
BASE COUNT          546 a         1518 c         1285 g          449 t
ORIGIN      
        1 cgaggacgcc acccaggagg cattcctggc cgccctgcgc aaggcggcca gcttccgcag
       61 ggcggccgcc ttctcgacct ggctctaccg gatcgccgtg aacgccgcca ccgaccaggc
      121 ccgccgccgg ggccgggccc ggctggccgc cctcgacccg gaggacgcgg gcctggccgt
      181 cgcccccggg agcgagctcg gcgaggtggt cgcctccgcc gtcgccgtcc agaccgccct
      241 gacccaggtc cccgaggagt tccgggtcgc ggtcgtcctc tgcgacctgt accgggttcc
      301 ctacgccgac gccgcccaga tcctcgaggt cccggtcggc accgtgaaga gccgcgtgtt
      361 ccgtggccgc ctcgccctcg ccgaatgcct cggcgcccct ccggacgccg atcccggcca
      421 cccaccactc cacgtggccg gccccccggg aaccgggacc gccccggcag cgtccgaacc
      481 tacagcgccc gacccgacca ggagcagctc cagcgtgccc aacctccacg accaccagcg
      541 gcagggccag ccccgcctgc ccgactccca cgaccacgag cgccaggggt agcccgaccg
      601 tgcccgaccg tcccgaccat cccgaccgcg agcagctcgc cgccttccag gccggcgacg
      661 gcgaacgccg ccagcggtcc gaggtcgagg cccatctggc cggctgcccg tcctgcgccg
      721 aggtggtcgc gtcggtcgcg cgggccagga gcggcctggc cctgctcgag gagccgatcc
      781 tcccggccgg cctgcacgac cgcgtggccg cggccgtcga ggccgaggcg gcccaggccg
      841 ccgccgacct tcccgcggcc cggcgtccgg aggcctcccc ggaggtcctc cgcccgaccc
      901 cctggtaccg ccggcccgtc gcctgggggg cggccgccgc cctgctcctc gccgccctgg
      961 tcgtcccatt cctcgaccag tccggcacga tgacctccgc cgaccgtgac gccgccggca
     1021 cggcctccca ggaggccgcc acggagggcg gcggcggggc cgccccgctc cccgtcatcc
     1081 ggatcccggg cgaggtcacg gcggcgaagg tccaggccac gctcaccgtc gaccgccgcg
     1141 ccaagacggc ccttgacgcc gccggcggcg gcacccccgc tgaccaggcc gcacccccca
     1201 gcggcagcct gtcgcgggag ttcgggcagc aggaggccgc ccgttcgagc accccagctc
     1261 cccaggccca cacggccctg ctcgcgtcct gcctccccgc cgccaccgga gacgccgacg
     1321 ccgccacccg ccccctcacc cccgccttct tcgtcgaggg cacctaccag ggccgcgagg
     1381 ccaccatcct cgtcaccacc agcgccagcc cgcccggccg ggtcgacctc tgggtgttcc
     1441 cccgcaacga ctgctccgcc ccacccgtgg ccaccgaacg ggtccggtag gacctcgttc
     1501 gcgctggggc ccccgcatcg gggcgggagt tctgctccac gctggggctc tggttcgcgc
     1561 cacggactat gctcgcgcgg ggctgtggac aacccggcgt acccgttccc ggagtggctg
     1621 tggacaaccc ggcgcaccgt tcccgcaggg ctgtggacaa cccggcgcac ccgttcccgt
     1681 ggtccgctac gctcccccac cgggggcccg gacccggcac tgggtcgctg aaggacgccc
     1741 gaacccagaa ccgggtcctt gcaggacgcc ccgaccctcg gaaccgggtc gctggaggag
     1801 cccgcccgtg ctaccaggcg ccgctggccc gggcgaacgc aaccccgccg ctgagcaggt
     1861 aggcgagcag gagccggccg gcgaacgggt tggcgccgag cagcagggcc aggccgccga
     1921 ggccgaccat gaccgcggcc gccgcggcca gcccgagcca ggggtgggtg cggcgccggg
     1981 ccgcccagcg cacggccgcc ccgaccacga agccatgggc gagggcgagc aggagcccgg
     2041 cgaacggcac cgccgccagc acgatcccgc cgaaggccgc gaccgccacg ccggccccga
     2101 accccctggc caggagctgc ggctccgggt tgcccttggc ccgccccgtc tgcttggcgc
     2161 agcgcgggca cttctgcccg accgcggtcg cgaccatgca gcgggtgcag atcgggtccc
     2221 cgcagttcga gcaggtgagc ctggtctcga ccttgggatg gttcacacag gtcggcgcca
     2281 ggttcgtctg gtcggcggtc ataggctaag cgtacgagaa ccacgccccg aacgtggccc
     2341 ctggccgacc cggcggtgga atagccatgt gggacaatcc gttgcagagc acggctcctg
     2401 aatccgccga cgtccgagga ggcgcgcgca ccccgatggc cacaccccga accaccgctc
     2461 cggtgctgat cgtcggctcc ggcccggccg ggctgaccgc ggccctctac accgcccgcg
     2521 ccaacctgca cccggttgtc ctcgaagggc tggaggccgg cggccagctc atgctcacca
     2581 ccgaggtcga gaactacccg gggttcccga ccgggatcct cggccccgag ctgatgggca
     2641 agatgcgcga gcaggccgag cgcttcggcg ccgagttcgt ccgggctgac gccaccaagg
     2701 tcgacctggc cggcccgccg ttccgggcct gggtcgagga ccaggagtac agcggcaagg
     2761 cgctgatcat cgccaccggc gccaaggcca agatgctcgg cctgcccggc gagagccagt
     2821 acctcggccg cggggtctcg acctgcgcca cctgcgacgg cttcttctac cgcaaccgcg
     2881 agctggtggt cgtgggcggc ggcgactccg cgctcgagga ggcgaccttc ctgaccaggt
     2941 tcgcgaccaa ggtcaccgtg gtccaccgcc gcgaccggct gcgcgcctcc aaggtcatgc
     3001 aggaccgggc cttcgccaac cccaagatcg ccttcgagtg gaactcggtc gtcgtcgacg
     3061 tgctcggcga gcagacggtc accggggtcc ggctgcgcaa cgtcgaggac ggcagcgaga
     3121 aggagctgcc ggtggccggg gtgttcgtgg ccatcggcca caccccgaac accgacctgt
     3181 tcaagagcca ggtcgaattc gacgaggccg gctacatcgt cacccccgac ccgccctcga
     3241 ccgcgaccag cgttgacggc gtgttcgcgg ccggcgacgt cgtcgaccac acctaccgcc
     3301 aggcggtgac cgccgccgga acgggatgcg cggccgcgat cgacgccgag cgctggctgg
     3361 aggcccacgg ccacgactcc ggggccacca ccgacaactg ggactgaagc tggttgccgg
     3421 acgctatcag tgggcaggaa tacgtaggag ccggtgatcg ttgcaccggt ggccgggggg
     3481 attcattcgt acgaggaggt ggaggtgcca aggggccgag gccccgaccg caccgacgat
     3541 atgaccaaag ccgcagccgt gaccgatgcc acgttcgaca acgaggtgct caagtcgagc
     3601 acgcccgtgc tggtcgactt ctgggccgag tggtgcggcc cctgcaagat gatcagcccc
     3661 ctgctcgagg agatcgccga cgagcacaag ggcaagctga ccatcgccaa gctcaacgtc
     3721 gacgagaacc ccgacgtggc catgcgctac ggcgtcatga gcatccccac cctcgccctg
     3781 ttcatcggcg gcgtggag
//
LOCUS       DASSWR010000158         4889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_769613,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000158 DASSWR010000000
VERSION     DASSWR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769613"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..764
                     /locus_tag="VFO47_02610"
     CDS             21..764
                     /locus_tag="VFO47_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET8787625.1"
                     /translation="MKGVILAGGTGSRLHPLTRITNKHLLPIYDRPMVTYAIETLVSA
                     GITELMVVTGGTHAGEFLRLLGNGHEYGIDRLLYAYQERPGGIAEALGLTERFVDGDR
                     VVVMLADNVVERSLRPAVTNFAAQESGARIVLARIDDPAHLRHLGVPELDGDGRVVRI
                     LEKPDDPPSSYVVTGIYFYDEHVFEVIPTLQPSGRGELEITDVNNWYVERRRMEYDVL
                     DGFWGDAGESIDAYYEVNDFVRVHGANKD"
     gene            852..1826
                     /locus_tag="VFO47_02615"
     CDS             852..1826
                     /locus_tag="VFO47_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2637 domain-containing protein"
                     /protein_id="HET8787626.1"
                     /translation="MALNGPPLITGIVATDKWLTRFRTILVAVLGATVLGIASLAFYT
                     SFEAIRAYAVRSNGIAPEHGWAVPLMVDSFIVVALGADLWFTTTKARRAWWEVWWPKL
                     LLAAAAGVSFVLNVAHAEHNWAARGVALIPPAALVLGVELLMMVLRRATTLRATRLQV
                     EAEAAQETAMAAPLARIQIEAMRRPRRPDTALGTATPAPPARPAPAEPKAGALPRPAG
                     RAPADTFRQAVAAAKGTDDPRPRRRQAPSMVASAILDERADADTLTPAQLVEAMAERG
                     VPISQSTAQALLRELRSPLSTRMATRRPTREPRPTGAGNGGRQAVRPK"
     gene            1972..2463
                     /locus_tag="VFO47_02620"
     CDS             1972..2463
                     /locus_tag="VFO47_02620"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HET8787627.1"
                     /translation="MVYLVRHAHAGTKAKWEGPDLARPLSAQGRKEALGLIERLRSFP
                     LGRVLSSPAERCLQTVQPLAARLGRPVEPYEVLGVDGSGAGVVELLEDPALDEAVLCT
                     HGEIIGEVFDELHKAGLKLSDDPGRPKGSTWVLRRDGGHAWKGIYLAPLAIEPEAQDS
                     LPR"
     gene            2500..3993
                     /locus_tag="VFO47_02625"
     CDS             2500..3993
                     /locus_tag="VFO47_02625"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HET8787628.1"
                     /translation="MKEREAKLAVPPGFDLPELGGLDDGFLAEPQPTRRLRTTYYDTA
                     DLRLARWDASLRYRPGEGWTVKLPEGQQGVLLVRAEHVFSGDGRRPPAEALALVRPFV
                     RTAGLSPSVRMRTLRRPVELREPGGSRLGELVDDEVQVLEGSRVLSRFRELEVELEED
                     ADLDLLEQVVDQLLDAGAQAADPTPKYLRALAGRERVLGPEVVQPEVDDGSSVEELLR
                     HDLATSTLRLFRHEAGVRIGEDPEAVHQARVATRRIRSTLRTFSKLLDEEWTDRLRDD
                     LKWLADLLGQVRDTDVLLERFEDHLAELPAEDAKAGRRLLAKLSDQRDQARRRLLGAM
                     AQEKYAVLLDDLVAAAAAPALLPGGDRPAVEVMPPMVTRPWKRLRKEVRKAGDDPPDE
                     VLHQIRIRAKRARYAAEAVEPVFGKPAEDFADAIADLQDVLGDHQDAVVGEAWLREAA
                     ASARRRDVAIVAGLLIAAERASAAGTRDRWRDVWKAASRKSLRAWLS"
     gene            3990..4334
                     /locus_tag="VFO47_02630"
     CDS             3990..4334
                     /locus_tag="VFO47_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET8787629.1"
                     /translation="MTDRREPDYRFSLANERTLLAWVRTALALDVAGLGVVRFAPPLG
                     GTGGRELVGGVLVLLGAVTAWSGYRRFLATDRAIRAGDALPAHAAPRVLAAALAVISV
                     VVLALLVGEQLR"
     gene            complement(4327..>4889)
                     /locus_tag="VFO47_02635"
     CDS             complement(4327..>4889)
                     /locus_tag="VFO47_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787630.1"
                     /translation="AERVGREAERLEVLVRDLHDLSRVERGTLDDEPVDLVGLAKEVA
                     GGYADLAEERRVKLRTELRPDVSVRGDRAQLGLLLSNLLDNALRHTPARGSVRVRLDT
                     VESRAVLQVADSGEGIPAGERSRVFERFYRVDKARARQTGGTGLGLAIVRHVAEAHGG
                     DVRVDSELGRGATFTVTLPVAGPPAT"
BASE COUNT          634 a         1932 c         1714 g          609 t
ORIGIN      
        1 atggaccgga aaggacctag atgaaaggcg tcatcctggc cgggggaact ggctcccggt
       61 tgcatcccct gacccgcatc accaacaagc acctgctgcc gatctacgac cggccgatgg
      121 tcacctacgc catcgagacg ctggtctcgg ccggcatcac cgagctgatg gtggtcaccg
      181 ggggcaccca cgccggcgag ttcctgcggc tgctcggcaa cggccacgag tacggcatcg
      241 accggctgct ctacgcctac caggaacggc cgggcggcat cgccgaggcc ctcggcctga
      301 ccgagcgctt cgtggacggg gaccgggtcg tggtcatgct ggccgacaac gtggtcgagc
      361 gcagcctgcg gccggccgtg accaacttcg ccgcccagga atccggcgcc cgcatcgtcc
      421 tggcccgcat cgacgacccg gcccacctgc gccacctcgg cgtccccgag ctggacggcg
      481 acggccgggt cgtccgcatc ctcgagaagc ccgacgaccc gccaagcagc tacgtcgtca
      541 ccggcatcta cttctacgac gagcacgtct tcgaggtgat cccgaccctg cagccgtccg
      601 gccggggcga gctggagatc accgacgtca acaactggta cgtcgagcgg cggcgcatgg
      661 agtacgacgt cctcgacggc ttctggggcg acgccggcga gtcgatcgac gcctactacg
      721 aggtcaacga cttcgtccgc gtccacgggg ccaacaagga ctgaaacgtc cgtaatgtgc
      781 gtcgcccggc ccgggtgcga tagaaccttg cgcgcttctg cccgaacccc gccccgagga
      841 gcgtgccgcc cgtggccctg aacggcccac ccctgatcac cgggatcgtc gccaccgaca
      901 agtggctgac gcggttccgg accatcctgg tcgccgtcct cggcgcgacc gtcctcggca
      961 tcgccagtct cgccttctac accagcttcg aggccatccg ggcctacgcc gtccgctcca
     1021 acggcatcgc ccccgagcac ggctgggccg tgcccctgat ggtcgactcc ttcatcgtgg
     1081 tcgccctcgg cgccgacctc tggttcacca ccaccaaggc gcgccgcgcc tggtgggagg
     1141 tgtggtggcc caagctgctg ctggccgcgg cggccggggt cagcttcgtg ctcaacgtcg
     1201 cccacgccga gcacaactgg gccgcccggg gggtcgccct catcccgccg gccgccctgg
     1261 tgctcggggt cgagctgctc atgatggtcc tgcggcgggc caccaccctg cgcgccaccc
     1321 ggctccaggt cgaggccgag gcggcccagg agacggccat ggccgcgccc ctggcccgca
     1381 tccagatcga ggccatgcgc cggccccgcc ggcccgacac cgcgctcggg accgccaccc
     1441 cggccccgcc ggcgcggccc gccccggccg agccgaaggc gggggcgctg ccccggccgg
     1501 cgggccgggc ccccgccgac accttccgcc aggccgtggc cgcggccaag ggcaccgacg
     1561 acccgcgacc ccggcgccgc caggccccct ccatggtcgc ctccgccatc ctcgacgagc
     1621 gggccgacgc cgacaccctc acccccgccc agctggtcga ggccatggcc gagcggggcg
     1681 ttcccatcag ccagtcgacc gcccaggcgc tcctgcgcga gctccgctcg cccctctcca
     1741 cccgcatggc cacccgccgt cccacccgtg agccccggcc gaccggcgcg ggcaacggcg
     1801 gccggcaggc ggtccggccc aagtagggcc ggccggccgg ccgggccagg gccgaacgga
     1861 cgatttccgc acgctccggg tcgtgcttga attgtcctgt cggtcgccat cggtcaagct
     1921 gggcacggac catggcgaac agggcgagat ggtcatgggg aggatgtgcc cgtggtctat
     1981 ctcgtgcgcc acgcccacgc gggaaccaaa gccaagtggg aggggcccga cctggcccgc
     2041 cccctgtcgg cgcagggccg caaggaggcg ctcgggctga tcgagcggct ccgctccttc
     2101 ccgctgggcc gcgtgctctc cagcccggcc gagcgctgcc tccagaccgt ccagccgctg
     2161 gccgcccggc tgggccgccc ggtggagccg tacgaggtcc tcggggtcga cgggtccggt
     2221 gccggcgtgg tcgagctgct ggaagacccg gcgctggacg aggccgtcct ctgcacccac
     2281 ggcgagatca tcggtgaggt cttcgacgag ctccacaagg ccggcctcaa gctctccgac
     2341 gatcccgggc ggccgaaggg ctccacatgg gtactccggc gcgacggtgg gcatgcatgg
     2401 aaggggatct acctggcgcc gctggcgatc gagccggagg cccaggactc gctcccacgg
     2461 tgaggcccgc aggcaaggcg cgagcaagga ggacggccga tgaaggagcg tgaggcgaag
     2521 ctggccgtcc cgcccggctt cgacctgccg gagcttggcg ggctggacga cggcttcctg
     2581 gccgagccgc agcccaccag acggctccgg accacctact acgacaccgc cgacctgcgg
     2641 ctggcccgct gggacgccag cctgcgctac cggcccgggg agggctggac ggtcaagctg
     2701 cccgagggcc agcagggggt cctgctggtc cgggccgagc acgtcttctc cggcgacggg
     2761 cgccggcccc cggccgaggc cctcgccctg gtccggccgt tcgtccgcac cgccggcctg
     2821 tcgccgtcgg tgcggatgcg caccctgcgc cgcccggtgg agctgcgcga gcccggcggg
     2881 tcgcggctgg gcgagctggt cgacgacgag gtccaggtgc tggagggcag ccgcgtcctc
     2941 agccgcttcc gcgagctcga ggtcgagctg gaggaggacg ccgacctcga cctgctcgag
     3001 caggtggtgg accagctgct cgacgccggc gcccaggcgg ccgacccgac ccccaagtac
     3061 ctgcgggccc tcgccggccg cgagcgggtg ctcgggcccg aggtcgtcca gccggaggtg
     3121 gacgacggct ccagcgtcga ggagctgctc cgccacgacc tggccacctc caccctgcgg
     3181 ctgttccggc acgaggccgg ggtccgcatc ggcgaggacc ccgaggccgt gcaccaggcc
     3241 cgggtcgcca cccgccgcat ccgctccacc ctgcggacct tctccaagct gctcgacgag
     3301 gagtggaccg accggctccg cgacgacctc aagtggctgg ccgacctgct cggccaggtc
     3361 cgcgacaccg acgtgctgct ggagcggttc gaggaccacc tggccgagct cccggcggag
     3421 gacgccaagg ccggacggcg gctcctggcc aagctgtccg accagcgcga ccaggcccgc
     3481 cggcggctgc tcggcgccat ggcccaggag aagtacgccg tgctgctcga cgacctggtc
     3541 gccgccgcgg ccgcgcccgc cctgctcccg ggaggcgacc gcccggccgt cgaggtgatg
     3601 ccgccgatgg tcaccaggcc gtggaagagg ctgcgcaagg aggtccgcaa ggccggggac
     3661 gacccgcccg acgaggtcct ccaccagatc cgcatccggg ccaagcgggc ccgctacgcc
     3721 gccgaggcgg tcgagcccgt gttcggcaag ccggccgagg acttcgccga cgccatcgcc
     3781 gacctccagg acgtcctcgg cgaccaccag gacgcggtcg tcggcgaggc ctggctccgc
     3841 gaggcggccg cctccgcccg gcggcgcgac gtcgccatcg tcgccggcct cctgatcgcc
     3901 gccgaacggg ccagcgccgc cggcacccgc gaccgctggc gcgatgtctg gaaggccgcc
     3961 agccgcaagt ccctgcgtgc ctggctctcg tgaccgaccg gcgcgagccc gactaccgct
     4021 tctccctggc caacgagcgg accctgctcg cctgggtgcg caccgccctg gccctggacg
     4081 tcgccgggct cggggtggtg cggttcgccc ccccgctcgg cggcaccggc ggccgcgagc
     4141 tcgtcggcgg cgtcctggtc ctgctgggcg ccgtcaccgc ctggagcggc taccgccgct
     4201 tcctcgccac cgaccgggcc atccgcgccg gcgacgccct cccggcccac gccgcccccc
     4261 gcgtcctcgc cgccgccctg gccgtgatct ccgtggtcgt gctcgccctg ctggtcggcg
     4321 agcagctcag gtagcgggcg gccccgccac cggcagggtg acggtgaagg tggcgccccg
     4381 gcccagctcg gagtcgaccc ggacgtcgcc gccgtgggcc tcggccacgt ggcggacgat
     4441 ggccaggccg aggccggtgc cgccggtctg gcgggcccgg gccttgtcga cccggtagaa
     4501 gcgctcgaac acccgcgagc gctcgccggc cgggatgccc tcgccgctgt ccgccacctg
     4561 gagcacggcc ctgctctcga cggtgtccag gcgcacgcgg acgctccccc tggccggggt
     4621 gtggcgcagg gcgttgtcga gcaggttgga cagcagcagg cccagctggg cccggtcgcc
     4681 gcggaccgac acgtccggcc tcagctcggt ccggagcttg acccgccgct cctcggccag
     4741 gtcggcgtag cccccggcga cctccttggc cagcccgacc aggtcgaccg gctcgtcgtc
     4801 gagggtcccg cgctccaccc gcgagaggtc gtgcaggtcc cggaccagga cctccagccg
     4861 ctcggcctcc cggccgaccc gctcggcga
//
LOCUS       DASSWR010000159         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_938822,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000159 DASSWR010000000
VERSION     DASSWR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938822"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /gene="nhaA"
                     /locus_tag="VFO47_02640"
     CDS             <1..1148
                     /gene="nhaA"
                     /locus_tag="VFO47_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET8787631.1"
                     /translation="HTQLTIGVGDWSLAEDLQHWINDALMALFFLVVGLEIKRELLTG
                     ELREPRVAALPAIAALGGMAVPALLYLAINSSGEPARGWGIPMATDIAFALGVLALVG
                     GKLPAALKSFLLALAIVDDIGAILVIAVFYSGSIEVLALLAAVGLLGLILILQRLHVR
                     WTVLYVLLGVGVWLAAFQSGVHATIAGVALGLATPAVPFQRPKAVSLEAHRIADDTVD
                     DPVPPDADAPQWLHLAGLTREAVSPLARTEHLLHPWTSYVIVPLFALANAGVSITSAT
                     LREALTSGVTLGVVVGLVVGKTVGVTAFTWAATRTGITRLPDGVRWSHLVGVAALAGI
                     GFTVSLFITSLAFQTPAVQNAAKVGILAASLLAGLLGALLLARFQRR"
     gene            complement(1389..1895)
                     /locus_tag="VFO47_02645"
     CDS             complement(1389..1895)
                     /locus_tag="VFO47_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET8787632.1"
                     /translation="MLDLDGAAQVVLTREGRRLLSERVRVLRVEVLPQLRAALEDPER
                     DGHVDAEFDRAVEELRRLTWVVDHAAIAEDVVGEPAVVELGTVVTIGVRQGVLERFLI
                     VHPIGAPLDDLRISVRSPLAQAVLGHQVGEEVEVAAPSGPYRCRIVAVESPPVGPDAA
                     PPTLDTTQ"
BASE COUNT          357 a          901 c          844 g          379 t
ORIGIN      
        1 ggcacaccca gctcaccatc ggagtcggtg actggtccct ggccgaggac ctccagcact
       61 ggatcaacga cgccctcatg gccctgttct tcctggtggt cggcctggag atcaagcgtg
      121 agctgctcac cggcgagctc cgcgagcccc gcgtggccgc cctacccgcc atcgccgcgc
      181 tgggcggcat ggccgtgccc gccctgctgt atctggccat caattccagt ggggagccgg
      241 cccggggctg gggcatcccg atggccaccg acatcgcctt cgccctgggg gtgctggccc
      301 tggtcggcgg caagctgccg gcggcgctca agtcgttcct gctcgcgctg gctatcgtgg
      361 acgacatcgg ggccatcctg gtcatcgccg tcttctattc cggcagcatc gaggtcctgg
      421 cattgctggc cgcggtcggg ctgctggggc tgatcctgat cctgcagcgg ctgcacgtgc
      481 gctggacggt gctgtatgtg ctgctcggcg ttggcgtgtg gttggccgcc ttccagtccg
      541 gggtccatgc caccatcgcc ggggtcgccc tgggcctggc cacccccgcg gttcccttcc
      601 agcgcccaaa ggcggtgagt ctcgaggcgc accggatcgc cgacgacacc gtggacgacc
      661 cggtcccgcc cgacgccgac gccccccagt ggctgcatct ggccggcctg acccgcgagg
      721 cggtctcgcc cctggccagg accgagcatc tgctgcatcc ctggaccagc tatgtgatcg
      781 tcccgctgtt cgcgctggcc aacgccgggg tgtccatcac cagcgcaacc ctgcgcgagg
      841 cgctgaccag cggcgtcacc ctgggggtgg tggtgggtct ggtggtcggc aagacggtcg
      901 gagtgaccgc attcacttgg gcggcgacga ggaccgggat cacccggctc cccgatgggg
      961 tccgctggag ccacctggtc ggggtcgccg ctctggctgg catcgggttc accgtgtcgc
     1021 tgttcatcac gagcctggcg ttccagaccc cagcggtcca gaatgcggcc aaggtcggca
     1081 tcctggctgc atcgctgctg gccggcctac tcggggcgct cctgcttgct cgtttccaga
     1141 ggaggtaggc cccagcgggc cgtgccaggc ggcggcagtc ctcctaccga cggcgcagag
     1201 ctcacgagcc gtcctcgggc gaaccgatcg ccaggccggc gatggcgccg atgacgatcc
     1261 agaacaagac attgcccagc cgagccccga tgccgacccc gacaccgccg ccaagcgcca
     1321 cccacgcttc gaccgcccgc tggctcgcgg ggactggagg ttgaccgcca ctcatgtccg
     1381 tccctcggtc actgcgtcgt gtcgagggtc ggcggcgccg catccggacc gacgggagga
     1441 ctctcgaccg ccacgatccg gcagcggtag gggcctgacg gggcagccac ttccacctcc
     1501 tcgccgacct ggtggccgag cactgcctgg gccaacggcg atcggaccga gatccgcagg
     1561 tcatccagcg gggccccgat cggatggacg atcaggaacc gctccagaac accctgcctg
     1621 actccaatag tcacgaccgt gcccagctcg accacggccg gttcgccgac cacgtcctcg
     1681 gcgatggcgg cgtggtccac cacccaggtc agacggcgca gctcctcgac cgcccgatcg
     1741 aactcggcgt cgacatggcc atcacgctca ggatcctcca aggcggcgcg cagctgtgga
     1801 aggacctcga cacgcaggac gcggacgcgc tcgctcagca gccgtctgcc ctcgcgggtc
     1861 agtaccacct gagcggcgcc atccagatcc agcatcgcca aggggtcacc gctcctcacg
     1921 accacctccc ggcctggacg cggaggccaa gcagatgcga ccgcccgaac gacggacctg
     1981 cgctgcgtgc cggccgttca ggcctgcagg cgcgaacgtt gcaactgccc ggtagacgct
     2041 catggccgaa gcacagccgc gtgcagtcga gggcgatcag ggcaaggatc gcgccgaggg
     2101 tggcgatcca acccaagagg ggaacatcca gcatgcaacc tccgagatct gccgccggcg
     2161 gacgaccaac agacgcctac cgatcgaggg ccgacctgcg ctggcggccg ggtctgtgcc
     2221 gctgcgctgt cctgcgcccc aggtgccggc tccccgtgag ttcaacgccg ttcgtcggag
     2281 ggtggcgggt cactgaggtt ggcaggtcgc gggctggtcc tggaccgccg gaacaacctg
     2341 cgcaccctcc cccgctggct gcccgatacc atcccggccg ggttagggtc ggacggatcc
     2401 aaagagaggg gttgaccgtc cgggcccgcg cgcgccgggg cggtgcggcc ggcgggtgtg
     2461 gcttcgtcgg cgggtgaggt c
//
LOCUS       DASSWR010000160         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1221856, whole genome shotgun sequence.
ACCESSION   DASSWR010000160 DASSWR010000000
VERSION     DASSWR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221856"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFO47_02650"
     CDS             complement(<1..569)
                     /locus_tag="VFO47_02650"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787633.1"
                     /translation="MAADPAPDRPAPPSALAPLARPVFRALWIAALVSNVGFWMQSVG
                     AVWQVGTVSGSAALVALVQTAISLPIVLLALPAGAAADVFDRRRMLLATQSWMLISAA
                     ALCLATVLGVASPAVVLGLTFMLGLGNAANAPAWQATIPELVGGRLLAPAVALNSAGF
                     NIGRAVGPAIGGLVVAAAGPAAVFGLNAVS"
     gene            596..>2028
                     /locus_tag="VFO47_02655"
     CDS             596..>2028
                     /locus_tag="VFO47_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8787634.1"
                     /translation="MASPIEDYALIGDTHTAGLVSREGSIDWLCLPRFDSPACFAALL
                     GERSNGRWLLAPAGPVREVRRRYEGDTLVLETEHHTDDGVVRVVDFMPPRQWDPDVAR
                     VVEGVRGRVPMRMELTIRFDYGSIVPWVRNVDGALHAVAGPDSVWLRTPVPVRGENWT
                     TLADFTVAEGERVPFMLTWNASHRPAPRRIDPIRALADTEAWWGEWASHVNYHGGWQD
                     AVLRSLLTLKALTYAPTGGIVAAPTTSLPEQLGGVRNWDYRYCWLRDATFTLSALMLA
                     GLAEEAKAWREWLLRAVAGQPRQMQILYGVAGERRITEQELDWLAGYEGARPVRIGNA
                     AVNQFQLDVYGEVMDTLHLGQHVGLESDEAAWDLQRALLEFLETKWREPDEGIWEIRG
                     PRRHFTHSKVMAWVGLDRAIKAVELLRHDGPVDRWRAVRRELHDEVCREGYDTERDSF
                     VQYYGADHLDASLLQIPLVGFLPAGDPR"
BASE COUNT          281 a          739 c          769 g          239 t
ORIGIN      
        1 gacacggcgt tgagcccgaa cacggccgcc gggccggccg ccgccaccac caggccgccg
       61 atcgccgggc cgaccgcccg gccgatgttg aacccggccg agttgagggc caccgccggg
      121 gccagcagcc ggcccccgac cagctcgggg atggtcgcct gccaggcggg ggcgttggcc
      181 gcgttgccca gcccgagcat gaaggtcagg ccgaggacga ccgccgggct ggccaccccg
      241 agcacggtgg ccaggcacag cgccgccgcc gagatcagca tccaggactg ggtggcgagc
      301 agcatccgcc gccggtcgaa cacgtccgcg gccgccccgg ctggcagggc cagcagcacg
      361 atgggcaggc tgatggccgt ctggacgagg gcgaccaggg cggccgagcc ggagacggtg
      421 ccgacctgcc agaccgcccc gacgctctgc atccagaagc cgacgttgga gaccagggcg
      481 gcgatccaga gggcccggaa caccggccgg gccagcggag ccagcgcgga cggcggggcg
      541 ggccggtcgg gggcggggtc ggcggccacg gcggcgctca gtgtagcttc gtcccatggc
      601 ctcccccatc gaggactacg cgctgatcgg cgacacccac acggccgggc tggtgagccg
      661 ggaggggtcg atcgactggc tgtgcctgcc ccggttcgac tcgccggcct gcttcgcggc
      721 cctgctcggc gagcggtcca acggccgctg gctgctcgcc ccggccggcc cggtccggga
      781 ggtccgccgc cgctacgagg gcgacaccct ggtgctggag accgagcacc acaccgacga
      841 cggggtcgtg cgggtggtcg acttcatgcc cccgcgccag tgggacccgg acgtggccag
      901 ggtcgtcgag ggggtcaggg gccgggtgcc gatgcggatg gagctgacca tccggttcga
      961 ctacggctcc atcgtgccct gggtgcggaa cgtcgacggg gcgctgcacg ccgtcgccgg
     1021 gcccgactcg gtctggctgc gcaccccggt gccggtgcgg ggcgagaact ggaccaccct
     1081 ggccgacttc accgtggccg agggtgagcg ggtcccgttc atgctcacct ggaacgcctc
     1141 ccaccggccg gccccgcgcc gcatcgaccc gatccgggcc cttgccgaca ccgaggcctg
     1201 gtggggcgag tgggcgagcc acgtcaacta ccacggcggc tggcaggacg cggtgctccg
     1261 gtccctgctc accctgaagg cgctgaccta cgcccccacc ggcgggatcg tggccgcccc
     1321 gaccacgtcg ctgcccgagc agctcggcgg cgtccgcaac tgggactacc gctactgctg
     1381 gctgcgcgac gccaccttca ccctgtcggc gctgatgctg gccgggctgg ccgaggaggc
     1441 caaggcgtgg cgggagtggc tgctgcgggc cgtggccggc cagccgcggc agatgcagat
     1501 cctctacggc gtggccggcg agcggcggat caccgagcag gagctggact ggctggccgg
     1561 ctacgagggg gcacggccgg tgcggatcgg caacgcggcc gtgaaccagt tccagctcga
     1621 cgtctacggc gaggtcatgg acaccctgca cctgggccag cacgtgggcc tggagagcga
     1681 cgaggcggcc tgggacctgc agcgggccct gctggagttc ctcgagacca agtggcgcga
     1741 gccggacgag ggcatctggg agatccgcgg cccccggcgg cacttcaccc actccaaggt
     1801 gatggcctgg gtggggctcg accgagccat caaggcggtc gagctgctgc gccacgacgg
     1861 ccccgtcgac cgctggcggg ccgtccgccg cgagctccac gacgaggtct gccgcgaggg
     1921 ctacgacacc gagcgggaca gcttcgtgca gtactacggg gccgaccacc tggacgccag
     1981 cctgctccag atcccgctgg tcgggttcct gcccgccggc gacccgcg
//
LOCUS       DASSWR010000161         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_995666,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000161 DASSWR010000000
VERSION     DASSWR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995666"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            740..1795
                     /locus_tag="VFO47_02660"
     CDS             740..1795
                     /locus_tag="VFO47_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET8787635.1"
                     /translation="MPDHVVVVGASAGGVEALVDLAASLPADLPAAMFVVLHVPSTAS
                     SALPDILSRHGPLRAGHGRDGEPIERGRIYVAPPDHHLLLRTGHVRLTRGPRENGHRP
                     AVDPLFRSAAREYASRVIGVVLSGALDDGTAGLLAIKSRGGVAVVQDPGDALYPGMPT
                     SAAEHAEVDHVLAAASMGRLLAKLTAEPATEPPGPAPTDMTVEVEMEGFSLEAMEGEH
                     PGEPSGFSCPDCNGVLWEIQTEGLRRYRCRVGHAWSPESLLIQQSEALEAALWIALRT
                     LEERAALARRLAEPARRRGHSVTATRFEEQATEAQQAARQVRNLLLDRGSLATAWPPA
                     TEHHPADSPSSTERSGG"
     gene            1788..>2367
                     /locus_tag="VFO47_02665"
     CDS             1788..>2367
                     /locus_tag="VFO47_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET8787636.1"
                     /translation="MAEPETDQDLEVLLDHLRRSRGFDFTGYKRASLSRRIHKRMQVV
                     GADSYLSYLDHLEVDPEEFTQLFNTILINVTSFFRDPPTWDYLRAEILPRLAESQPDG
                     EPIRMWSAGCASGEEAYSLAMATAEVLGADTVRERVKIYATDVDEDALNQARQARYTP
                     RQVEGVPPELLERYFERNGHGHVFSKDLRAGPW"
BASE COUNT          353 a          827 c          858 g          329 t
ORIGIN      
        1 tgccgggtgg tgcggtgacg ccgcgggcca ttggttactg aagggacgca tcgacggggt
       61 ctgggccgac acgccgctcc aggcgataca gccgcccgag cgggtagcta ggcccaaagc
      121 ggccaccccc gaggctaccc ttggacaggc tggaagctgc ggccaccgca ggttcctgtt
      181 gggcacatcc taatcaacgt caccggcgcg gtcggcctag gctgatcggg gtggagcatg
      241 cttgccaagt tcgccgactg tcgtcatcgt cgagcgtgca gcgcaaggca cgcctgacgc
      301 acggcctgtt gaggtgccaa tggatgtcga cggcggaaag cagccggacg agccggcccc
      361 gcctgtccga gtggagccgg gggtggaccg gctggcgcag gtgcgcgacc gcctcaccca
      421 ggccagacgt cgtgagctcg ccgcccacca gcgcgccatc gagctgcacg agcgggccgc
      481 cgagctgcag gggcagttgg gtcggtccga tcgggcggct agtgctcgga ggcatgctca
      541 gcacgctcgg gagttatacg agcaggcccg cgccgaggga gcgcaggacc ggtaggtccg
      601 gccggcggtc gagaggcgtg cctatggacg gggaactgtc gagctgcgca tcatggccga
      661 acgtcgggcc tggtctcgtt aggatcgagg tgcctgggta cccataagcc gccatcactt
      721 ctgcgttcag gaggccgtca tgcccgacca cgtggtggtc gtcggcgcct cggccggcgg
      781 ggtggaggcg ctcgtcgacc tggccgcctc gctcccggcg gacctgcccg cggccatgtt
      841 cgtggtcctg catgtgccct cgactgctag cagcgccttg ccggacatcc tgagcaggca
      901 cggcccgctg cgggccgggc atgggcggga tggcgagcca atcgaacgtg ggcgcatcta
      961 tgtcgcgcct cctgatcacc atctgctgct acggaccggc catgtgcgcc tgacccgcgg
     1021 gccgcgcgag aacgggcatc ggccggccgt cgaccccctg ttccgcagcg ccgcccggga
     1081 gtacgcgtcc agggtgatcg gcgtggtcct gtcgggcgcc ctggacgacg gtaccgccgg
     1141 gctgctggcg atcaagtcac gcggtggcgt cgcggtcgtc caggacccgg gggacgcgct
     1201 ctatccgggg atgccgacca gcgcggccga gcacgccgag gtcgaccatg tcctcgcggc
     1261 ggcctccatg ggcaggctgc tcgccaagct gaccgccgag ccggcaacgg agccgcccgg
     1321 gccagcgccg accgacatga ccgtggaggt cgagatggag ggcttctcgc tcgaggccat
     1381 ggagggcgag catccgggtg agccgtcggg gttctcctgt cccgactgca acggtgtgct
     1441 ctgggagatc cagaccgagg ggctgcggcg gtaccgctgc cgggtcggcc acgcgtggtc
     1501 gcccgagagc cttctgatcc agcagagcga ggccctggag gcggcgctgt ggatcgccct
     1561 gcggacgctc gaggagcggg ccgccctggc cagacggctg gccgagccgg cccggcgccg
     1621 cgggcactcc gtcacggcca cccgcttcga ggagcaggcg accgaggccc agcaggccgc
     1681 ccggcaggtc cgcaacctgc tgctggaccg gggcagcctg gccaccgctt ggccaccggc
     1741 taccgagcac cacccggcgg attcgccgtc ttcgacggag aggtccggtg gctgagccgg
     1801 agaccgacca ggacctggag gtcctgctcg accacctgcg tcgcagccgc gggttcgact
     1861 tcaccggcta caagcgggcc agcctgagcc gccgcatcca caagcgcatg caggtcgtcg
     1921 gggccgacag ctacctgagc tacctggacc acctcgaggt cgaccccgag gagttcaccc
     1981 agctgttcaa caccatcctg atcaacgtca ccagcttctt ccgggacccg ccgacctggg
     2041 actacctgcg cgccgagatc ctgccccgcc tggccgagag ccagcccgac ggcgagccga
     2101 tccggatgtg gagcgccggc tgcgcctccg gcgaggaggc gtactcgctg gccatggcga
     2161 ccgccgaggt gctcggggcc gacacggtgc gcgagcgggt caagatctac gccaccgacg
     2221 tcgacgagga tgccctcaac caggcccgcc aggcccgcta caccccccgg caggtggaag
     2281 gggtcccgcc ggagctgctg gagcgctact tcgagcgcaa cgggcacggc cacgtcttct
     2341 ccaaggacct gcgcgcaggt ccttgga
//
LOCUS       DASSWR010000162         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_684928,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000162 DASSWR010000000
VERSION     DASSWR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684928"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1231)
                     /locus_tag="VFO47_02670"
     CDS             complement(62..1231)
                     /locus_tag="VFO47_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787637.1"
                     /translation="MPVDPELLSGLRAAVEAVPDNLALRLHVATLLLEAGRPAESLEH
                     SSAVLALEPGQREAAQLVERATAALPDTPFPMPAPFSAAPPDPQHRGATMDQWVLGEV
                     EEPRTTLADVGGMPAIKRWLATAFLSSLRDPQMRRLFGRSLRGGLLMYGPPGCGKTFL
                     ARAVAGELDAGFLRIDLGHVVDMWLGYSRRSIHEIFETARASAPCLLYLDQLDAVGPR
                     RAGRRHSIGRGVVDQLVAELYSASEEHEGVFVVAATEHPWDVDALLRRPGRLDHRLLV
                     LPPDRQAREAIIRSVLDGRPLAEDLDVAVLAARTDKYSAADLAQLCESAAAAALEASM
                     VSNSSRPIALEDFTRGLHEIRPSTPPWFDLARDYARFAAEPGSYDDLVSYLRARG"
     gene            1350..2363
                     /locus_tag="VFO47_02675"
     CDS             1350..2363
                     /locus_tag="VFO47_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8787638.1"
                     /translation="MSSIHFVDALLADADRDQRAAPGPTDDEALDAYSQVVTGVAERV
                     IGSVASLRISRRLPGGRQAQGSGSAVAITPDGFLVTSAHVVEGAGKGRASLADGRELR
                     FEVVGRDALSDLAVIRASGADLEPVSLGNADRLRVGQLVVAVGNPMGLAGSITAGVVS
                     ALGRSFPTSDGRTSRLIENVIQTDASLNPGNSGGALVDGRGRMVGVNTAVAGVGLGLA
                     VPINATTRRIIAALMREGRFRRAFIGIAGGSRPLPPRVAQVLDRAAGVEVVQVVDGSP
                     AARAGLRNEDLIIDVDGVAVTDAGDLQRLMVGERIGRPLVVRAFRGGELLELTVVPVE
                     LGP"
     gene            2467..>3289
                     /locus_tag="VFO47_02680"
     CDS             2467..>3289
                     /locus_tag="VFO47_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET8787639.1"
                     /translation="MAAVAVLAGGGWLFLAGRDAPPEARLRPGAGDWGSGRPDGEWVV
                     APDADGFVGYRVRERLGSISAPNDVVGRSPGVSGTVTIAGGGRHRAEVTVDMTRLRTD
                     VEPRDGRMREEGLETDRFPTASFRLREPVPLGDVSGARVVDLRLPGNLTLHGVTRPLD
                     VPVQARWDGDSIQVAGGTRIRRADFDLEVPTLAGYRIEDSPVVELELTLVREGAALAG
                     SASTLRRATAVPQPGGEEPGRPQAEPGQGGGRLPEGDGRLLFSAITDDDTEHLFTI"
BASE COUNT          415 a         1209 c         1275 g          390 t
ORIGIN      
        1 gcagtaagat actctcacca ctcagatttt gtgaacatct gagttggagc ggggccgccg
       61 gctacccgcg ggcccgcagg tagctgacca ggtcgtcgta ggacccgggc tcggcggcga
      121 agcgggcgta gtcccgggcc aggtcgaacc agggcggggt gctggggcgg atctcgtgca
      181 gcccgcgggt gaagtcctcc agggcgatcg gccgcgagct gttgctgacc atcgaggcct
      241 cgagggcggc cgcggccgcc gactcgcaga gctgggccag gtcggcggcg gagtacttgt
      301 cggtgcgggc ggccaggacg gccacgtcca ggtcctcggc cagcgggcgg ccgtccagga
      361 cggagcggat gatggcctcg cgggcctggc ggtcgggcgg caggacgagc aggcggtggt
      421 cgaggcggcc gggccggcgc agcagcgcgt cgacgtccca ggggtgctcg gtggccgcga
      481 ccacgaacac cccctcgtgc tcctcgctgg cgctgtacag ctcggccacg agctggtcga
      541 cgaccccccg cccgatcgag tggcggcggc cggcccggcg ggggccgacg gcgtcgagct
      601 ggtcgaggta gagcaggcag ggcgcgctgg cccgcgccgt ctcgaagatc tcgtggatgc
      661 tgcggcggct gtagccgagc cacatgtcga ccacgtgccc gaggtcgatg cggaggaagc
      721 ccgcgtccag ctccccggcc acggcccggg ccaggaacgt cttgccgcac ccgggcggcc
      781 cgtacatcag caggccgccg cggagcgacc ggccgaacag ccggcgcatc tgcgggtcgc
      841 gcagcgacga caggaaggcg gtggccagcc agcgcttgat cgccggcatg ccgccgacgt
      901 cggccagggt cgtccgcggc tcctcgacct cgcccagcac ccactggtcc atggtggccc
      961 cccggtgctg gggatcgggc ggggccgcgg agaacggggc cggcatcggg aacggggtgt
     1021 cgggcagggc ggccgtggcc cgctcgacca gctgggcggc ctcgcgctgg ccgggctcga
     1081 gggcgagcac ggccgagctg tgctccagcg actcggcggg gcggcccgcc tccagcagca
     1141 gcgtggcgac gtgcagacgc agggccaggt tgtcgggcac ggcctcgacc gccgcccgca
     1201 gaccggaaag cagctcaggg tcgaccggca tggcggcaac agtatgcaat ccgcccgaga
     1261 cgcgcgcgaa acgttcgccg ccggtcggtc ggcgaaacgc tgattacagt attacgatga
     1321 ggaggaccta ctcggggagg gaggcgacga tgagctccat ccacttcgtc gacgcactgc
     1381 tggccgatgc cgaccgcgac cagcgggccg cgcccggccc aacagacgac gaggccctcg
     1441 acgcctactc ccaggtggtc accggcgtcg ccgagcgggt gatcggctcg gtggccagcc
     1501 tgcggatcag ccgccggctg cccgggggac gccaggcgca gggctccggc tcggcggtgg
     1561 cgatcacccc ggacggcttc ctggtcacct cggcccacgt ggtcgagggg gccggcaagg
     1621 gccgggcctc gctggccgac ggccgcgagc tgcgcttcga ggtcgtcggc cgcgacgcgc
     1681 tgtccgacct ggcggtgatc cgggcgagcg gggccgacct ggagccggtg tccctgggga
     1741 acgccgaccg gctccgggtc ggccagctcg tcgtggccgt cggcaacccc atggggctgg
     1801 ccgggtccat caccgccggg gtggtcagcg ccctcggccg ctcgttcccg accagcgacg
     1861 gccggaccag ccgcctgatc gagaacgtga tccagaccga cgcgtccctc aaccccggca
     1921 actccggcgg cgccctggtc gacgggcgcg gccgcatggt cggggtcaac accgccgtgg
     1981 ccggggtcgg cctcggcctg gccgtgccca tcaacgccac cacccggcgg atcatcgccg
     2041 ccctgatgcg cgagggccgc ttccggcggg ccttcatcgg catcgccggc ggcagccgcc
     2101 ccctccctcc gcgggtcgct caggtgctgg accgcgccgc cggggtcgaa gtcgtccagg
     2161 tggtcgacgg cagcccggcg gcccgagccg ggctgcgcaa cgaggacctg atcatcgacg
     2221 tcgacggcgt ggccgtgacc gacgccggcg acctccagcg cctgatggtg ggcgagcgca
     2281 tcgggcggcc gctggtcgtc cgcgccttcc ggggcggcga gctgctggag ctgaccgtgg
     2341 tcccggtcga gctcggacct taacgttcgc tgagaatcgc caccgacccg cccggcgcgg
     2401 gaaccacgcc tgggcggcgg gcgtaccggg ggcatggggc ctctgccgag acgcgcgctc
     2461 gccgccgtgg cggccgtggc cgtgctggcc ggcggcggct ggctgttcct ggccggccgc
     2521 gacgccccgc ccgaggcccg gctgcggccc ggcgccggcg attggggcag cggccgtccc
     2581 gacggcgagt gggtcgtggc cccggacgcc gacgggttcg tcggctaccg ggtccgcgag
     2641 cggctgggat cgatctcggc cccgaacgac gtcgtcggcc gctcccccgg cgtcagcggc
     2701 accgtcacca tcgccggcgg gggccgtcac cgggccgagg tgaccgtgga catgacccgc
     2761 ctgcggaccg acgtcgagcc gcgggacggg cgcatgcgcg aggagggcct ggagacggac
     2821 cgcttcccga cggccagctt ccgcctgcgc gagccggtcc cgctgggcga cgtgtccggg
     2881 gccagggtgg tcgacctgcg cctccccggg aacctcaccc tccacggggt gacccggccg
     2941 ctcgacgtcc ccgtgcaggc ccgctgggac ggggacagca tccaggtcgc cggcggcacc
     3001 cggatccgcc gggccgactt cgacctggag gtccccaccc tggccggcta ccggatcgag
     3061 gacagccccg tggtcgagct ggagctcacc ctggtccgcg agggcgccgc cctggccggc
     3121 tcggccagca ccctgcggcg ggccacggcc gtcccccagc ccggcgggga ggagccgggc
     3181 cgcccccagg ccgagcccgg ccagggcggc gggcgcctgc ccgaggggga cgggcggctg
     3241 ctgttcagcg ccatcaccga cgacgacacc gagcacctgt tcaccatcg
//
LOCUS       DASSWR010000163         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_967373,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000163 DASSWR010000000
VERSION     DASSWR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967373"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="gatB"
                     /locus_tag="VFO47_02685"
     CDS             <1..1284
                     /gene="gatB"
                     /locus_tag="VFO47_02685"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET8787640.1"
                     /translation="AVEFAARIGLALHCRIAERSQFHRKNYFYPDMPKNYQISQYDEP
                     LCVGGWLDVDVEGEVRRVGITRVHLEEDTGKSLHVGATGRIHGADYSLVDYNRAGIPL
                     VEIVSEPDLRSAEEARAYAEELRAVLLALGVSDAKLEEGSMRFDVNVSIRPVGHAEFG
                     TKVELKNLNSLRSLYRAVAYEADRQRRVLADGGRLVQETRHWDEGAGRTEPMRSKEFA
                     TDYRYFPEPDLIPIEATAPWVDGLRADLPELPAERRARLVEATGLPAKEVGWLARDPE
                     VLAYFQAVATGRDPKVVASWVMGELQRDLREFDHTMASNPVEPERLGGLLDLLSAGTV
                     SATAAKDVLAEMFTSEASPATIVERKGLAQISDTGELEAVVERVVAANPDLADKFRGG
                     KRGVLGALVGQVMRETRGRANPKLVSDLLERAIGG"
     gene            1326..2807
                     /locus_tag="VFO47_02690"
     CDS             1326..2807
                     /locus_tag="VFO47_02690"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET8787641.1"
                     /translation="MTTHPQVLLERYEVGRLLGAGGMAEVFEGRDRLLARRVAIKVPL
                     SQYAHDPEFAQRFRREAQAAASLSHPGVVAVYDTGAENGTHFIVMEFVDGRTLKDVIR
                     AEAPLYPDRAAEITADVCSALAAAHARGLVHRDVKPANIMLMPDGRVKLMDLGIARVA
                     ASETVTQTAAMLGTAQYLSPEQAQGQAVDYRSDLYSLGCCLYEMLTGTVPFRGATPVA
                     IAYRHVREDPAAPRLLNPDIPPALEAVCLKAMAKRPEDRYQTAAEFRADLERARAGQR
                     VAAGPAAAGAATAAMATTVLPPLGGYPGAPDPTAAFGGPVAPSRAARHAEPPPGPRRR
                     WWLYVLVPLGVLALGVGVAFVVSRLVGGLPETPAASTLPTATAPQTVPTTLLRTSTSQ
                     LPTTSRPPTTTAPPTTAPPTTQQQPNQVQVPDVIGRRARVARAQLEAAGLRVNQQQVP
                     VRDSQQAGRVVLQNPQAGTTVGRDSTVTIVVGLRFGDDGDGDG"
BASE COUNT          385 a         1130 c         1054 g          346 t
ORIGIN      
        1 gccgtcgagt tcgcggcccg catcgggctg gccctgcact gccggatcgc cgagcggtcc
       61 cagttccacc gcaagaacta cttctatccg gacatgccca agaactacca gatctcccag
      121 tacgacgagc cgctgtgcgt cggcggctgg ctcgacgtgg acgtggaggg cgaggtccgg
      181 cgggtcggga tcacccgggt ccacctggag gaggacaccg gcaagagcct ccacgtcggg
      241 gccaccgggc ggatccacgg ggccgactac tcgctggtcg actacaaccg ggccgggatc
      301 ccgctggtcg agatcgtgtc cgagcctgac ctgcgctcgg ccgaggaggc ccgcgcgtac
      361 gccgaggagc tgcgggcggt gctcctggcc ctcggggtct ccgacgccaa gctcgaggaa
      421 ggctcgatgc ggttcgacgt gaacgtgtcg atccggccgg tcggccacgc cgagttcggc
      481 accaaggtcg agctgaagaa cctcaactcg ctgcgcagcc tgtaccgggc ggtcgcctac
      541 gaggccgacc ggcagcgccg ggtcctggcc gacggcggcc ggctcgtcca ggagacccgc
      601 cactgggacg agggggcggg ccggaccgag ccgatgcggt ccaaggagtt cgccaccgac
      661 taccgctact tccccgagcc cgacctgatc cccatcgagg ccaccgcccc ctgggtcgac
      721 gggctccggg ccgacctgcc cgagctgccg gccgagcgcc gggcccggct ggtggaggcg
      781 accggcctgc ccgccaagga ggtcggctgg ctggcccgcg accccgaggt cctcgcctac
      841 ttccaggcgg tcgccaccgg gcgcgacccc aaggtggtgg ccagctgggt gatgggcgag
      901 ctccagcgcg acctgcgcga gttcgaccac accatggcct cgaacccggt ggagccggag
      961 cggctcggcg ggctgctcga tctcctctcc gccggcaccg tctcggccac cgcggccaag
     1021 gacgtgctgg ccgagatgtt cacctcggag gcgtcaccgg ccaccatcgt ggagcgcaag
     1081 ggcctggccc agatctccga caccggcgag ctggaggcgg tggtcgagcg ggtggtggcc
     1141 gccaaccccg acctggccga caagttccgc ggcggcaagc ggggcgtgct cggcgccctg
     1201 gtcggccagg tcatgcgcga gacccgcggc cgggccaacc ccaagctggt cagcgacctg
     1261 ctggaacggg ccatcggcgg ctgagccaac gcccgcatac cgggacgtat aagctgctgg
     1321 gcgccatgac cacccacccc caggtgctcc tggaacgcta cgaggtcggc cggctgctcg
     1381 gcgcgggcgg catggccgag gtcttcgagg gccgcgaccg gctgctggcc cgccgggtcg
     1441 ccatcaaggt gccgctgtcc cagtacgccc acgacccgga gttcgcccag cggttccggc
     1501 gggaggcgca ggcggcggcc agcctgtcgc atcccggggt ggtcgccgtc tacgacaccg
     1561 gcgccgagaa cggcacccac ttcatcgtca tggagttcgt ggacggccgc accctcaagg
     1621 acgtgatccg ggccgaggcg ccgctgtacc cggaccgggc ggccgagatc accgccgacg
     1681 tctgctcggc cctggccgcc gcccacgccc gcggcctggt ccaccgcgac gtcaagccgg
     1741 ccaacatcat gctcatgccc gacggccggg tgaagctgat ggacctgggg atcgccaggg
     1801 tggccgcctc cgagacggtc acccagaccg cggccatgct cggcaccgcc cagtacctgt
     1861 cgcccgagca ggcccagggg caggcggtcg actaccgcag cgacctgtac tcgctcggct
     1921 gctgcctgta cgagatgctc accggcacgg tgccgttccg cggggcgacc ccggtggcca
     1981 tcgcctaccg gcacgtccgc gaggacccgg ccgcgccgcg gctgctcaac cccgacatcc
     2041 cgccggccct ggaggcggtc tgcctcaagg cgatggccaa gcggcccgag gaccgctacc
     2101 agacggcggc cgagttccgc gccgacctgg agcgggcccg ggccgggcag cgggtcgcgg
     2161 ccgggccggc cgccgccggg gcggccacgg cggccatggc gaccacggtc ctgccgccgc
     2221 tgggcggcta cccgggtgcc cccgacccga ccgcggcctt cggcgggccc gtcgccccga
     2281 gccgcgccgc ccgccacgcc gagccgccgc cgggcccgcg ccggcgctgg tggctgtacg
     2341 tgctcgtgcc gctgggggtg ctggccctcg gggtgggggt cgcgttcgtg gtctcgcggc
     2401 tggtcggcgg gctgcccgag acccccgcgg cctcgaccct gcccacggcc accgcgcccc
     2461 agacggtccc caccaccctc ctgcggacct cgacgtcgca gctccccacc acctcgcggc
     2521 cgcccaccac caccgcgccg ccgaccaccg ccccccccac cacccagcag cagccgaacc
     2581 aggtccaggt gccggacgtg atcggccgcc gcgcccgggt ggccagggcc cagctggagg
     2641 cggccgggct ccgggtcaac cagcagcagg tcccggtccg cgactcccag caggccggcc
     2701 gggtcgttct ccagaacccg caggccggga ccaccgtcgg ccgggactcc accgtcacca
     2761 tcgtggtcgg cctccgcttc ggcgacgacg gcgacggcga cggctgagcg gccatggcgg
     2821 cggcgcgggt ggtgctgttc ggggccaccg ggtacacggg ccggctgacg gccgaggccc
     2881 tggccgcggc cggggcgagg ccggtgctgg ccggc
//
LOCUS       DASSWR010000164         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_967391,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000164 DASSWR010000000
VERSION     DASSWR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967391"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFO47_02695"
     CDS             complement(<1..569)
                     /locus_tag="VFO47_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8787642.1"
                     /translation="MQQASRAPFSVIVGLGRTGVSAARHLAARGHHLVVTDSRADPPG
                     LAELRRLVPGAATALGGFDPAVLEGADQVVVSPGVSLADPFLRTAAARGLDLVGDIEL
                     FAREAGGRVVGITGTNGKSTVTTLVGAFAKAAGLEVRVGGNLGLPALDLLEGPPADAI
                     ARVAKVADADEIIVGSRGLGRFRGAFGSVS"
     gene            complement(569..1615)
                     /gene="mraY"
                     /locus_tag="VFO47_02700"
     CDS             complement(569..1615)
                     /gene="mraY"
                     /locus_tag="VFO47_02700"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET8787643.1"
                     /translation="MVGILIAAAVALALGLVGTPLAIRTFRRRGLGQLIQVDLPDAHQ
                     TKRGTPTMGGAVIILGAVAGYLVVHLFSGGRVPFTPAGALVVITMVLLGIVGFVDDYL
                     KIRNQRSLGLTKTAKFAGQALAAIVLGVGAWYWADAFRGISFIRPLGGTELHLILFLV
                     WVFLLVSATSNGVNLTDGMDGLASGSTVLVVAAYVVIGFWQFRHLCGSPPDTAGCYRF
                     PAGESPQDLAIVAAAMMGSTAGFLWWNAPPARIFMGDTGSLALGGTLAALALFTNTQL
                     LLAVLGGLYVVETLSVILQVASFRLRGKRIFRMAPIHHHYELKGWAEPKVIVRFWIIS
                     FMLVLIGLATLKLR"
     gene            complement(1619..3007)
                     /gene="murF"
                     /locus_tag="VFO47_02705"
     CDS             complement(1619..3007)
                     /gene="murF"
                     /locus_tag="VFO47_02705"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET8787644.1"
                     /translation="MIPVTLKEIAEVTGGRLAGADPDAVVTGVQVDSRKVQPGELFVA
                     LPGSRTDGSLFAAAAAEAGAAATLAQEGTVFAGPRVEVADPLAALGALGTAVRDRSAA
                     TVVAVTGSNGKTTTKDLLAAALATRLRTVANQASFNNEVGLPLTLTRIEPGTQAVVVE
                     MGARGPGHIAALARLARPGVGVVLNVGESHLGMFGSREAIAKAKGELVEALPPDGTAV
                     LNADDPQVAAMAGRTVARVVTFGQAAGEVRAAGVELDGDGRARFLLHTPAGTAPVVLP
                     APGEHLVGCALAAAAAAHVLGVGPDAAAAGLAAARLSPMRMQVHRRPDGLTVVNDAYN
                     ANPSSMAAALKTLAALGRQGGRTVAVLGEMAELGPDAAAEHDRIGRLATRLGIDRLVG
                     VGEPGRVMAAAARMEGMWPEEAEAVPDADAAVALLTPALGPDDVVLVKASRVVALDRV
                     ADALLRPRPEGV"
     gene            complement(3004..>3571)
                     /locus_tag="VFO47_02710"
     CDS             complement(3004..>3571)
                     /locus_tag="VFO47_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET8787645.1"
                     /translation="GLEALAGVPGRFERVDAGQPFTVLVDYAHTPDSLDNLLRAARAV
                     TGGRVIVVFGCGGDRDRAKRPLMGEIAGRLADVAVVTSDNPRSERPEAIVEQVAQGVA
                     RAAGPDGFLVEVDRRAAIRAALAMAGPGDAVLLAGKGHEQGQEFAGGGKVPFDDRVVA
                     TEELRALLGGPGGAWSEAVAPRESGAAR"
BASE COUNT          480 a         1306 c         1398 g          387 t
ORIGIN      
        1 gacacgctcc cgaacgcccc gcggaagcgt ccgagccccc gcgaccccac gatgatctcg
       61 tccgcatcgg cgaccttggc gacacgggcg atcgcgtcgg ccggcgggcc ctcgagcagg
      121 tcgagcgcag gcaggccgag gttgccgccg acgcgcacct cgagcccggc cgccttcgcg
      181 aaggcgccga ccagcgtcgt gaccgtgctc ttgccgttgg ttcccgtgat gccgaccacg
      241 cggccgccgg cctcgcgcgc gaacagctcg atgtcgccga cgaggtcgag cccccgcgcc
      301 gcggccgttc tcaggaaagg atcggcgagc gagacgccgg gcgagacgac gacctggtcc
      361 gcgccctcga gcaccgccgg gtcgaaaccc ccgagcgccg tcgcggcgcc gggcacgagg
      421 cggcgcagct ccgcaagccc gggcgggtcc gcgcgggagt cggtcacgac caggtgatgg
      481 ccgcgcgcgg cgaggtggcg cgcggccgaa acgccggtgc ggccgaggcc gacgatcacg
      541 ctgaagggcg cgcggctcgc ctgctgcatc agcgcagctt cagcgtggcc agcccgatga
      601 ggaccagcat gaacgagatg atccagaagc ggacgatgac cttcggctcc gcccagccct
      661 tcagctcgta gtggtggtgg atcggcgcca tccggaagat gcgcttgccg cgcagccgga
      721 acgacgccac ctggaggatc accgagaggg tctcgaccac gtacagcccg cccagcacgg
      781 ccagcagcag ctgggtgttg gtgaacaggg ccagggcggc cagggtgccg ccgagggcca
      841 gggagccggt gtcgcccatg aagatgcggg ccgggggggc gttccaccac aggaacccgg
      901 ccgtcgagcc catcatggcc gcggccacga tggccaggtc ctggggcgac tcgccggccg
      961 ggaaccggta gcagccggcg gtgtcgggcg ggctcccgca caggtggcgg aactgccaga
     1021 acccgatgac cacgtaggcg gccaccacca gcacggtgga gccggaggcg agcccgtcca
     1081 tgccgtcggt caggttgacc ccgttggagg tcgccgagac gagcaggaac acccagacca
     1141 ggaacaggat gaggtggagc tcggtgccgc cgagggggcg gatgaaggag atgccgcgga
     1201 aggcgtcggc ccagtaccag gcgccgaccc cgagcacgat cgcggccagg gcctggccgg
     1261 cgaacttggc cgtcttggtc aggccgaggc tgcgctggtt gcggatcttg aggtagtcgt
     1321 cgacgaagcc gacgatgccg agcaggacca tggtgatgac gaccagggcc ccggccgggg
     1381 tgaacgggac ccggccgccg ctgaacaggt ggacgaccag gtagccggcg acggcgccga
     1441 ggatgatcac cgccccgccc atggtcgggg tgccgcgctt ggtctggtgg gcgtcgggca
     1501 ggtcgacctg gatgagctgg ccgagcccgc gccgccggaa ggtccggatg gccaggggcg
     1561 tgccgaccag gcccagggcg agggcgacgg cggccgcgat caggatcccg accaacggtc
     1621 agaccccttc cggccgggga cgaagcaggg cgtcggcgac ccggtcgagg gcgacgaccc
     1681 ggctggcctt gaccaggacc acgtcgtcgg ggccgagggc aggggtgagc agggccacgg
     1741 cggcgtcggc gtcggggacc gcctcggcct cctcgggcca catgccctcc atgcgggcgg
     1801 cggcggccat cacccggccc ggctccccca ccccgaccag ccggtcgatg cccaggcggg
     1861 tggccaggcg gccgatgcgg tcgtgctcgg cggccgcgtc cgggcccagc tcggccatct
     1921 cgccgagcac ggccaccgtg cgcccgccct ggcggccgag ggcggccagg gtcttgaggg
     1981 ccgccgccat cgacgacggg ttggcgttgt aggcgtcgtt gaccacggtc agcccgtcgg
     2041 gccggcggtg gacctgcatc cgcatgggcg acaggcgggc cgcggccagg ccggcggcgg
     2101 cggcgtccgg cccgaccccg aggacgtggg cggccgcggc cgcggccagg gcgcagccga
     2161 ccaggtgctc gccgggggcg ggcaggacga ccggggcggt cccggccggg gtgtggagca
     2221 ggaaccgggc ccggccgtcg ccgtccagct cgaccccggc ggcccgcacc tcgccggcgg
     2281 cctggccgaa ggtgaccacc cgggccacgg tgcggccggc catggccgcc acctgggggt
     2341 cgtcggcgtt gagcacggcc gtgccgtcgg gcggcagggc ctcgacgagc tcgcccttgg
     2401 ccttggcgat cgcctcccgg gagccgaaca tgcccaggtg ggactcgccg acgttgagga
     2461 ccacgccgac cccgggccgg gccaggcggg ccagggcggc gatgtggccg gggccgcggg
     2521 cgcccatctc gaccaccacc gcctgggtgc cgggctcgat ccgggtgagg gtcaggggca
     2581 ggccgacctc gttgttgaac gaggcctggt tggcgaccgt gcgcaggcgg gtggccaggg
     2641 cggcggcgag caggtccttg gtggtggtct tgccgttgga gccggtgacc gccaccaccg
     2701 tggccgccga ccggtcccgc acggccgtgc ccagggcgcc gagggcggcc agggggtcgg
     2761 cgacctcgac ccgggggccg gcgaagacgg tgccctcctg ggccagggtc gcggccgcgc
     2821 cggcctcggc ggcggcggcc gcgaacaggc tgccgtcggt gcggctcccg ggcagggcca
     2881 cgaacagctc ccccggctgg accttgcggg agtcgacctg gaccccggtg acgacggcgt
     2941 ccgggtcggc gccggccagc cgccccccgg tcacctccgc gatctccttg agcgtgaccg
     3001 ggatcaacgt gccgcccccg attcccgggg ggctaccgct tcgctccatg cgccccccgg
     3061 accccccagc agcgcccgca gctcctcggt ggccacgacg cggtcgtcga aggggacctt
     3121 gccgccgccg gcgaactcct ggccctgctc gtggcccttg ccggcgagca ggacggcgtc
     3181 gcccggcccg gccatggcca gggcggcccg gatggcggcc cggcggtcga cctcgaccag
     3241 gaacccgtcc ggaccggccg cgcgggccac cccctgggcg acctgctcga cgatcgcctc
     3301 cgggcgctcg gagcggggat tgtcggaggt gaccacggcc acgtcggcca gccggccggc
     3361 gatctcgccc atcagcggcc gcttggcccg gtcgcggtcg ccgccgcagc cgaagaccac
     3421 gatcacccgc ccgccggtga ccgcccgggc cgcccggagc aggttgtcga gggagtcggg
     3481 ggtgtgggcg tagtcaacca gcacggtgaa gggctggccg gcgtcgaccc gctcgaagcg
     3541 gcccggcacc ccggccaggg cctccagccc g
//
LOCUS       DASSWR010000165         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_685092,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000165 DASSWR010000000
VERSION     DASSWR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685092"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VFO47_02715"
     CDS             complement(<1..1529)
                     /locus_tag="VFO47_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787646.1"
                     /translation="MTATLTSPTFVGRTEELARLAEATDRAAAGTPAAVLIGGEAGVG
                     KTRLVGEVVAAARRAGATVLVGGCVELGGEGVPFAPLIEALRGFVRDLDEPELARLLS
                     DQARAELARLLPELDPPGADSRLEPDRLTTTPGPWTDQGRLFEQLLGLLERLSADLPA
                     VLVVEDLHWADRSTRDLLAFLVRNLRHGRLLLVLTYRSDELHRRHPLRPFLAELDRGR
                     RVERLELARFGPAEVAAQLAGIQGAPPPAGLAERIHARSGGNAFFVEELAATAAADGE
                     LPPSLRDTLLARIELLGEPAQQVLAVAAAAGSRVEHELLAEVAAIDEAALLAGLREAV
                     SAQVLLVDAGDGAYGFRHALVKEAVYAELLPGERTRLHARFAAALAARDGGGDPGLAA
                     ELAWHWYAAHDLDRALPAAVEAGKAAERAYAFAEAQTQFERALELWDRCATATQRPGG
                     LDKAELLARAAEAASNAGAADRAVALVRGALAEVDQAKDPLLAAQLLERLAFHLRIAG
                     RP"
     gene            complement(1629..1760)
                     /locus_tag="VFO47_02720"
     CDS             complement(1629..1760)
                     /locus_tag="VFO47_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787647.1"
                     /translation="MQQHHIFHDAIARDHVEVLRRDARQARLAKARRKSARSSRRSR"
     gene            1953..3071
                     /locus_tag="VFO47_02725"
     CDS             1953..3071
                     /locus_tag="VFO47_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET8787648.1"
                     /translation="MATHEIRVDYGKTLLEEPETGHNRWHPDIPPVVTCDPGDEVVLE
                     TRDAFDGQMGPEATLETVAGPNLNVVHPLTGPVYVNGAEAGDLLEVEILDVEPDRYGY
                     TVQVPGFGFLRDQFPDPYMARWRIEGGWATSDDLPGLRIPGSPFMGTIGLSPDRALLE
                     RTTAREQAALDRGGFVLPPDPTDAVPSDPAIAAEALRTIPPRETAGNVDIKQLGKGTR
                     LYIPVYADGGLFSAGDAHFAQGDCETCGTAIEMRSTLTVRFALHKGRAAARGIQDLQF
                     ARDDYYLPPEYAAPRRFFATTGLSVTRDGESRPEDLTLAARNALLNMIDHLQDHGGWT
                     RQQAYAICSVAVDLKVSEVVDVPSFVVSAFLPLDIFQG"
     gene            complement(3068..>4771)
                     /locus_tag="VFO47_02730"
     CDS             complement(3068..>4771)
                     /locus_tag="VFO47_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HET8787649.1"
                     /translation="MHPFAHGQGTQTPLEALVSAHQRRYPRADVRLLHQAYEVAEREH
                     RGQQRTSGEPFITHPVAVAAILAELGMDTTTLVAALLHDTVEDTGYTLEQTRADFGDE
                     VTHLVDGVTKLDKMEFGHAAEAETIRKMIVAMALDLRVLIIKLADRLHNMRTLRFQPA
                     HKQQRTARATLEVLAPLANRLGLQVIRRDLEDLAFATLDPAEHDEIVRVVDSRDPGRR
                     EHVAMLVRQVAADLRAAKIKALVTPHPRHYYSIYQTMLERGSREIHDPDRILVVVAGG
                     PSDCYIALGAVHGRWHPVPGRFKDFIAAPKLNGYQSLHTTVIGPGEEPVEVHIRTEAM
                     HRTAEYGVVAAAREAARGRRANGQGDSTGGADDLVWLHRLLDWQRAVSDPGEFLESLR
                     SDLSDNEVLVFTPKGDALTLPAGATPVDMAYALRTELGHHCIGASVNGQLVPLASVLA
                     DGDVVEILTSTAEYPGPSKEWLGFVKSPQAHVKIRQWLASQRRDDAVDAGQQAIDRVL
                     ADQGWSLQRAMEDGSLAMLDYADLEAMYRAVGQEQLSAQSVAQWLVTVLGDQPQGNAE
                     R"
BASE COUNT          584 a         1760 c         1787 g          640 t
ORIGIN      
        1 ggccggccgg cgatgcgcag gtggaaggcc aggcgttcga ggagctgggc ggccagcagc
       61 gggtccttgg cctggtcgac ctcggccagg gcgccgcgga ccagggccac ggcccggtcg
      121 gcggcgccgg cgttggaggc cgcctccgcc gcccgggcca gcagctccgc cttgtccagc
      181 ccgccagggc gctgcgtcgc ggtggcgcac cggtcccaca gctccagggc ccgctcgaac
      241 tgggtctggg cctcggcgaa ggcgtaggcg cgctcggccg ccttgccggc ctcgaccgcc
      301 gccggcaggg cccggtccag gtcgtgggcg gcgtaccagt gccaggccag ctcggcggcc
      361 agcccggggt cgcccccgcc gtcgcgggcg gccagggcgg ccgcgaaccg ggcgtgcagg
      421 cgggtccgct cccccggcag cagctcggcg tagaccgcct ccttgaccag cgcgtggcgg
      481 aagccgtagg cgccgtcgcc ggcgtcgacc agcagcacct gggccgagac ggcctcgcgc
      541 aggccggcca gcagcgccgc ctcgtcgatg gcggccacct cggccaggag ctcgtgctcg
      601 acccggctcc cggcggccgc ggccacggcc agcacctgct gggccggctc gcccagcagc
      661 tcgatgcggg ccagcagggt gtcgcggagg ctggggggca gctcgccgtc ggcggcggcc
      721 gtggccgcca gctcctcgac gaagaaggcg ttgccgccgg agcgggcgtg gatccgctcg
      781 gccagcccgg ccggcggcgg cgccccctgg atcccggcca gctgggccgc cacctcggcc
      841 gggccgaacc gggccagctc cagccgctcg acccgccggc cgcggtccag ctcggccagg
      901 aacggccgca ggggatggcg gcggtgcagc tcgtcgctgc gataggtcag caccagcagc
      961 aggcggccgt ggcgcaggtt gcggacgagg aaggcgagca ggtcgcgggt ggagcggtcg
     1021 gcccagtgca ggtcctccac gaccagcacg gccggcaggt cggcgctcag gcgctccagc
     1081 aggccgagca gctgctcgaa cagccggccc tggtccgtcc aggggcccgg ggtcgtggtc
     1141 agccggtcgg gctccagccg gctgtcggct cccggcgggt ccagctcggg caggaggcgg
     1201 gccagctcgg ccctggcctg atcggagagc agccgggcca gctccggctc gtccaggtcc
     1261 cgcacgaacc cccgcagggc ctcgatcagc ggggcgaacg gcaccccctc gccgccgagc
     1321 tcgacgcagc cgccgaccag cacggtcgcc ccggcccggc gggcggcggc gacgacctcg
     1381 ccgaccagcc gggtcttgcc caccccggcc tcgccgccga tcagcaccgc cgccggggtc
     1441 cccgcggcgg cccggtcggt ggcctcggcc agccgggcca gctcctcggt ccggccgacg
     1501 aaggtggggc tggtgagcgt cgcggtcatg cccacgagta tcgcttggcc ggccgacgaa
     1561 gcgaacgagc cggcgggttc gccggctcgt cgctgtcgct cgcgcgtgac cacgggcatg
     1621 ggtgcctcct agcggctccg gcgggacgag cgggcgctct tgcggcgggc cttggccagc
     1681 cgggcctgcc gggcgtcgcg gcggaggact tcgacgtggt cgcgggcgat cgcgtcgtgg
     1741 aagatgtggt gctgctgcat cgttggcctc ctggtcggtt ccgctctctc gatcgtcacg
     1801 gtatggctaa gaccccccgc cccggatcgg acggttgcct taggttcccg gcccccctac
     1861 ctcatcttgg ggagggggct gcttaggagg tggactttgg acccggcaag gcaagtatcg
     1921 ttgtgccccc gtcggacctg gaggtggtga ccatggcgac gcacgagatc cgcgtcgact
     1981 acggcaagac gctcctggag gagcccgaga ccgggcacaa ccgctggcat ccggacatcc
     2041 cgccggtggt cacctgcgac cccggcgacg aggtggtgct ggagacccgc gacgccttcg
     2101 acggccagat ggggccggag gcgaccctgg agacggtggc cgggcccaac ctgaacgtgg
     2161 tccaccccct gaccggcccg gtgtacgtca acggggccga ggccggcgac ctgctcgagg
     2221 tcgagatcct cgacgtcgag cccgaccgct acggctacac cgtgcaggtg cccggcttcg
     2281 ggttcctgcg cgaccagttc cccgacccgt acatggcccg ctggcggatc gagggcggct
     2341 gggcgacctc cgacgacctg ccggggctgc gcatccccgg ctccccgttc atgggcacca
     2401 tcggcctttc ccccgaccgg gccctgctgg agcggaccac cgcccgcgag caggccgccc
     2461 tcgaccgggg cgggttcgtg ctcccccccg accccaccga cgccgtcccc tcggaccctg
     2521 ccatcgccgc cgaggccctg cgcaccatcc cgccccggga gacggccggc aacgtcgaca
     2581 tcaagcagct cggcaagggg accaggctct acatcccggt gtacgccgac gggggcctgt
     2641 tctcggccgg cgacgcccac ttcgcccagg gcgactgcga gacgtgcggc acggccatcg
     2701 agatgcgctc gaccctgacc gtccgcttcg ccctccacaa gggccgggcc gccgcccgcg
     2761 gcatccagga cctccagttc gcccgcgacg actactacct gccgcccgag tacgccgccc
     2821 cccgccggtt cttcgccacc accggcctgt cggtcacccg cgacggcgag agccgccccg
     2881 aggacctcac cctggccgcc cgcaacgccc tgctcaacat gatcgaccac ctccaggacc
     2941 acggcggctg gacccgccag caggcctacg ccatctgcag cgtcgccgtc gacctcaagg
     3001 tctcggaggt ggtggacgtc cccagcttcg tcgtctcggc gttcctgccc ctggacatct
     3061 tccagggcta gcgctcggcg ttgccctgcg gctggtcgcc gaggacggtg accagccact
     3121 gggcgaccga ctgggccgag agctgctcct ggccgacggc gcggtacatg gcctccaggt
     3181 cggcgtagtc gagcatggcc agggagccgt cctccatggc ccgctggagc gaccagccct
     3241 ggtcggccag gacccggtcg atcgcctgct ggccggcgtc gacggcgtcg tcgcggcgct
     3301 gggaggccag ccactggcgg atcttgacgt gggcctgggg gctcttgacg aagccgagcc
     3361 actccttgga cgggcccggg tactcggccg tcgaggtgag gatctcgacc acgtccccgt
     3421 cggccaggac gctggccagg gggacgagct ggccgttgac gctggcgccg atgcagtggt
     3481 ggcccagctc ggtccggagc gcgtaggcca tgtcgaccgg ggtggcgccg gccggcaggg
     3541 tgagggcgtc gcccttgggg gtgaacacca gcacctcgtt gtcggacagg tcggagcgga
     3601 gcgactccag gaactcgccc gggtcggaca cggcccgctg ccagtccagc agccggtgca
     3661 gccagaccag gtcgtcggcg ccgccggtgg agtcgccctg gccgttggcc cggcggccgc
     3721 gggcggcctc gcgggcggcc gcgaccaccc cgtactcggc cgtgcggtgc atggcctcgg
     3781 tgcggatgtg gacctccacc ggctcctcgc cggggccgat cacggtggtg tgcagcgact
     3841 ggtagccgtt gagcttgggg gcggcgatga agtccttgaa gcggccgggc accgggtgcc
     3901 agcgcccgtg caccgccccc agggcgatgt agcagtcgct gggcccgccg gcgaccacga
     3961 ccaggatccg gtcggggtcg tggatctcgc ggctgccccg ctccagcatc gtctggtaga
     4021 tcgagtagta gtgccgcggg tggggggtga ccagggcctt gatcttggcc gcgcgcaggt
     4081 cggccgcgac ctggcggacc agcatggcca cgtgctcgcg gcgtcccggg tcgcggctgt
     4141 cgaccacccg cacgatctcg tcgtgctcgg ccgggtcaag ggtcgcgaag gccaggtcct
     4201 ccaggtcgcg gcggatcacc tggagcccga gccggttggc caggggggcc agcacctcca
     4261 gggtcgcccg ggccgtgcgc tgctgcttgt gggcgggctg gaaccggagg gtgcgcatgt
     4321 tgtggagccg gtcggccagc ttgatgatca gcacccgcag gtccagggcc atggccacga
     4381 tcatcttgcg gatggtctcg gcctcggcgg cgtggccgaa ctccatcttg tcgagcttgg
     4441 tcaccccgtc gaccaggtgg gtgacctcgt cgccgaagtc ggcccgggtc tgctccaggg
     4501 tgtagccggt gtcctccacg gtgtcgtgga gcagggccgc gaccagggtg gtggtgtcca
     4561 tgccgagctc ggccaggatg gccgccacgg ccaccgggtg ggtgatgaac ggctccccgc
     4621 tggtccgctg ctggccccgg tgctcccgct cggcgacctc gtaggcctgg tgcagcaggc
     4681 gcacgtcggc cctgggatag cgccgctggt gagcggagac cagcgcctcc agcggggtct
     4741 gggtgccctg gccgtgggcg aaggggtgca t
//
LOCUS       DASSWR010000166         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_119909,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000166 DASSWR010000000
VERSION     DASSWR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119909"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFO47_02735"
     CDS             complement(<1..300)
                     /locus_tag="VFO47_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8787650.1"
                     /translation="MTRVLTVATWNIFGGRTWEGARVDLDLTLATLRRLDADLVAVQE
                     VDRDQARSHGADQACLLGEALDMEWRYAPALLGTPGNPEGWRAPAPGADDPGGTAY"
     gene            complement(297..1085)
                     /locus_tag="VFO47_02740"
     CDS             complement(297..1085)
                     /locus_tag="VFO47_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET8787651.1"
                     /translation="MAQGVVTDLARWPVKSLGGEALGAARLDWRGLGGDRAHALLDLR
                     EGRTGRWVTIRQAPRMLAWSAAYPDAPDDRLDPADPPPPVVTAPDGARWGWDDPGLER
                     ALADDLGVPVRRHRDLGGQQDLQASVPVTTEASRRAVGDALGRPVELRRFRTNLHLDL
                     DAPAFAEERWEGGRLQVGEVTLELGHPCSRCVIPTRHPDDLSRWPELLRWLHRRRRAL
                     FGVNARVVTAGRVRVGDRVTVTEPAVPVPPRPSTEWDVAPRGVL"
     gene            complement(1086..>2256)
                     /locus_tag="VFO47_02745"
     CDS             complement(1086..>2256)
                     /locus_tag="VFO47_02745"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET8787652.1"
                     /translation="IRSRRDPRQPPGLEVADFEEYTRLYWEAWGDPVLRWLLSTVPTA
                     MIFDDHDISDDWNTSAAWVEHMRAMPWWEERITSGLASYWLYQHLGNLSPEDLDDDPV
                     WPKVREAGDATRVLREFAARADQEIEGTRWSYKRDFGRTRLVVIDSRCGRDLEGDRRE
                     MIDDDEFAWVEEQATGDFDHLLLATSLPFALAPAIHHLEEWNEAVTAGAWGRPLAWLG
                     EKIRQGIDLEHWAAFNDSFDRLAALIESVAAGHRGRPPGSVVVLSGDVHHTYLAELSY
                     RGQHAGHDHSPAWQAVCSPLRNPLPRDQRAAHVRAFRRPARAVTRWLARRAGVEPDEI
                     AWRVVEGPWFENHLGQLELDGRAAMLRVEQAVTPDGGDGDPYLEPLYDHRLSDGR"
BASE COUNT          264 a          870 c          847 g          275 t
ORIGIN      
        1 gtacgccgtg ccgccggggt cgtcggctcc gggcgccggg gcgcgccagc cttcggggtt
       61 gcccggggtg ccgagcaggg ccggggcgta gcgccactcc atgtcgaggg cctcgccgag
      121 caggcaggcc tggtcggccc cgtggctgcg ggcctggtca cggtcgacct cctggaccgc
      181 gaccaggtcg gcgtccaggc gccgcagggt ggccagggtc aggtccaggt cgacccgggc
      241 cccctcccag gtccggcccc cgaagatgtt ccaggtggcc accgtcagca cccgggtcac
      301 aaaacccccc tcggggcgac gtcccactcg gtcgagggcc gcggcgggac cgggaccgcg
      361 ggctccgtca cggtcacccg gtcgccgacc cggacccggc cggcggtcac cacccgggcg
      421 ttgaccccga acagggccct gcggcggcgg tgcagccagc gcagcagctc cggccagcgg
      481 gacaggtcgt cggggtggcg ggtcgggatc acgcagcggc tgcaggggtg gcccagctcc
      541 agggtgacct ccccgacctg gaggcggccg ccctcccagc gctcctcggc gaaggcgggc
      601 gcgtccaggt cgaggtgcag gttggtccgg aagcgccgca gctcgaccgg ccggccgagg
      661 gcgtcgccga cggcccgccg cgacgcctcg gtggtcaccg ggaccgacgc ctggaggtcc
      721 tgctggccgc cgaggtcgcg gtggcgccgg acgggcacgc cgaggtcgtc ggccagggcc
      781 cgctccaggc cggggtcgtc ccagccccag cgggcgccgt caggggcggt cacgaccggc
      841 ggcggcgggt cggccgggtc caggcggtcg tcgggcgcgt ccgggtacgc cgccgaccag
      901 gccagcatcc gcggcgcctg ccggatcgtc acccaccgcc ccgtgcgacc ctcgcgcagg
      961 tcgagcaggg cgtgggcgcg gtcgccgccc agcccccgcc agtcgagccg ggccgcgccc
     1021 agcgcctccc cgcccagcga cttcaccggc caccgggcca ggtcggtcac caccccctgc
     1081 gccacctatc ggccgtccga cagccggtgg tcgtacaggg gctccaggta cgggtcgccg
     1141 tcgccgccgt cgggggtgac ggcctgctcg accctgagca tggccgcccg gccgtccagc
     1201 tccagctggc cgaggtggtt ctcgaaccac ggcccctcga ccacccgcca ggcgatctcg
     1261 tccggctcga cgccggcccg gcgggccagc cacctggtca cggcccgggc cgggcgccgg
     1321 aacgccctga cgtgggcggc ccgctggtcg cggggcagcg ggttgcgcag cggcgagcag
     1381 accgcctgcc aggccgggct gtggtcgtgg ccggcgtgct ggccgcggta ggagagctct
     1441 gccaggtacg tgtggtggac gtcgccggac aggaccacca ccgacccggg gggccggccc
     1501 cggtggccgg ccgccaccga ctcgatcagc gccgccagcc ggtcgaagga gtcgttgaac
     1561 gcggcccagt gctccaggtc gatcccctgc cggatcttct cccccagcca ggccaggggt
     1621 cggccccagg ccccggcggt caccgcctcg ttccactcct cgaggtggtg gatggccggg
     1681 gccagggcga acggcagcga ggtggccagc agcaggtggt cgaagtcgcc ggtggcctgc
     1741 tcctccaccc aggcgaactc gtcgtcgtcg atcatctcgc ggcggtcgcc ctccaggtcc
     1801 cgcccgcagc gcgagtcgat caccaccagc ctggtccggc cgaagtcgcg cttgtagctc
     1861 cagcgggtcc cctcgatctc ctggtcggcc cgggcggcga actcgcgcag cacccgggtg
     1921 gcgtcgcccg cctcccgcac cttgggccag accgggtcgt cgtccaggtc ctccggggac
     1981 aggttgccca ggtgctggta cagccagtag gaggcgaggc cgctggtgat ccgctcctcc
     2041 caccacggca tggcccgcat gtgctcgacc caggcggccg aggtgttcca gtcgtcgctg
     2101 atgtcgtggt cgtcgaagat catggccgtg ggcaccgtcg acagcagcca gcgcagcacc
     2161 gggtcgcccc aggcctccca gtacagccgc gtgtactcct cgaagtcggc cacctccagg
     2221 cccggcggct ggcgcgggtc gcggcgcgag cggatg
//
LOCUS       DASSWR010000167         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1193199, whole genome shotgun sequence.
ACCESSION   DASSWR010000167 DASSWR010000000
VERSION     DASSWR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193199"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFO47_02750"
     CDS             <1..302
                     /locus_tag="VFO47_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787653.1"
                     /translation="VLVFDHRLAEHRPLDSKSELARLLFGYTTGNGQRFPAQADLVRF
                     VARPGQDNDVQDVLAGPDAIARAEGGEVINFVYRAEGRRTEGTLARIGNGELRVR"
     gene            506..1672
                     /locus_tag="VFO47_02755"
     CDS             506..1672
                     /locus_tag="VFO47_02755"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET8787654.1"
                     /translation="MRTARRIDAIPPYLFAEIDRKIAERKAAGVDVISFGVGDPDLPT
                     PDFVVEELARAAGDPSTHQYPSYFGLPSFRRAIADFYRRRFGVELDPDTQVLPLIGSK
                     EGIAHLPWAFVDPGDQVLVTEPGYPVYEVGTILAGGEPVHIPLTAEDGWFPDFAAVGP
                     ATAPRAKLLWLCYPSNPTGAVATADQLQEAVRFAADRDLLLAYDNAYSEITYDGFVAP
                     SVLQVPGAMDVAVEFGSLSKIFNMTGWRIGWAVGSPVAIEALGRVKTNIDSGIFNAVQ
                     QAGVVALESGMPHLPGLLETYRRRRDRIMEVFWDAGWKVDAPKGALYVWLPTPPGESS
                     VDFTARLLDEAGVVVAPGTGYGPSGEGYIRLSITIPDSRLDEGCERVRKALVGR"
     gene            1734..>2586
                     /gene="hflX"
                     /locus_tag="VFO47_02760"
     CDS             1734..>2586
                     /gene="hflX"
                     /locus_tag="VFO47_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET8787655.1"
                     /translation="MSEHTLTEARRADGGSATTPTRPADEVKLVELRKLRLERVVLVG
                     VAVDGSVERAERSVEELHRLAETAGAEVLDTMVQRRARPDAGTFIGKGKAKELAATVR
                     ALEADTVIMDDELSPGQLRHLEELVDAKVIDRTVLILDIFAQHATSREGKAQVELAQL
                     NYMLPRLRGWGESLTRVGSGMGSGGPIGTRGPGETKMEVDRRRIRRRITKLRRDLVDM
                     ARTRDVKRRGRERAGVPAVALVGYTNAGKSTLLNRLSGAGVLVEDKLFSTLDPTTRRL
                     DLPGGRAA"
BASE COUNT          360 a          975 c          917 g          334 t
ORIGIN      
        1 acgttctcgt cttcgatcac cgtctcgccg agcaccggcc gctcgactcc aagtccgagc
       61 tggcccggct cctgttcggc tacaccaccg gcaacggtca gcgcttcccg gcccaggccg
      121 acctggtccg gttcgtggcc cgccccggcc aggacaacga cgtccaggac gtcctggccg
      181 ggccggacgc gatcgcgcgg gcggagggag gcgaggtcat caacttcgtc taccgggccg
      241 aggggcggcg caccgagggc accctggccc ggatcggcaa cggcgagctg cgggtccgct
      301 gagccgccac caggcgaagc atcgggtagc agccgctcgc cggagccccc gatttcggcc
      361 gttcgccggg tgcctgcgac cgaccgcttg gccaggcccc ggccgccggc tgccgggcgg
      421 agcgccgggg cgcggtgggc ggtaggctgc tggtttccga tcgccagatc gtgccggacc
      481 tccacgcgag gagactgccc agctcatgcg caccgcacgc cgcatcgacg ccatcccgcc
      541 gtacctgttc gccgagatcg accggaagat cgccgagcgc aaggcggcgg gggtcgacgt
      601 catctcgttc ggcgtcggtg accccgacct gcccaccccg gacttcgtgg tcgaggagct
      661 ggccagggcg gccggcgacc cctccaccca ccagtacccg agctacttcg ggctgccgtc
      721 gttccggcgc gcgatcgccg acttctaccg gcgccgcttc ggggtcgagc tcgacccgga
      781 cacccaggtc ctgcccctga tcggctccaa ggaggggatc gcccacctgc cgtgggcgtt
      841 cgtcgacccg ggcgaccagg tgctggtgac cgagcccggc taccccgtgt acgaggtcgg
      901 gaccatcctg gccggcggcg agccggtgca catcccgctc accgccgagg acggctggtt
      961 ccccgacttc gccgccgtcg gcccggccac ggcgccccgg gccaagctgc tgtggctctg
     1021 ctacccgtcc aacccgaccg gcgcggtcgc caccgccgac cagctccagg aggcggtccg
     1081 gttcgcggcc gaccgcgacc tgctgctcgc ctacgacaac gcctactccg agatcaccta
     1141 cgacggcttc gtcgccccgt cggtcctcca ggtgccgggc gccatggacg tggccgtcga
     1201 gttcggctcg ctgtccaaga tcttcaacat gaccggctgg cggatcggct gggcggtcgg
     1261 cagcccggtg gccatcgagg ccctcggccg ggtcaagacc aacatcgact ccggcatctt
     1321 caacgccgtg cagcaggccg gggtcgtcgc cctggagagc ggcatgccgc acctgcccgg
     1381 gctgctggag acctaccggc gccgccgcga ccggatcatg gaggtgttct gggacgccgg
     1441 ctggaaggtc gacgccccca agggcgccct ctacgtgtgg ctgcccaccc cgccggggga
     1501 gtcgagcgtc gacttcaccg cccggctgct ggacgaggcc ggtgtggtcg tcgcccccgg
     1561 caccggctac ggcccgagcg gcgagggcta catccgcctg tccatcacca tccccgacag
     1621 ccgcctcgac gagggctgcg agcgggtccg caaggcgctg gtcggccgct aggcggacca
     1681 ggccggtggg aaatggcggg acggatcggg ggcgacccgc tacggtggat gggatgagtg
     1741 agcacacatt gaccgaagcc cgccgcgccg acggcgggtc cgcgaccacc cccacccgcc
     1801 cggccgacga ggtcaagctc gtcgagctgc gcaagctccg cctcgaacgg gtggtgctcg
     1861 tcggcgtggc cgtcgacggg tcggtcgagc gcgccgagcg ctcggtcgag gagctgcacc
     1921 gcctggccga gacggccggg gccgaggtgc tggacaccat ggtccagcgg cgggcccgcc
     1981 ccgacgccgg caccttcatc ggcaagggca aggccaagga gctggccgcg accgtcaggg
     2041 cgctcgaggc cgacaccgtc atcatggacg acgagctgtc cccggggcag ctccggcacc
     2101 tggaggagct ggtcgacgcc aaggtgatcg accgcaccgt gctgatcctc gacatcttcg
     2161 cccagcacgc caccagccgg gagggcaagg cccaggtcga gctcgcccag ctcaactaca
     2221 tgctgccccg gctgcgcggc tggggcgaga gcctcacccg ggtgggcagc ggcatgggct
     2281 cgggcgggcc gatcgggacc cgcgggcctg gcgagaccaa gatggaggtc gaccgccgcc
     2341 gcatccgccg ccggatcacc aagctccgcc gcgacctggt cgacatggcc aggacccggg
     2401 atgtcaagcg ccgcggccgc gagcgggccg gcgtgcccgc ggtcgccctg gtcggctaca
     2461 ccaacgccgg caagtcgacc ctcctgaacc ggctgtcggg ggccggggtg ctggtcgagg
     2521 acaagctgtt ctcgaccctc gaccccacca cccggcggct cgacctgccc ggcgggcggg
     2581 ccgcga
//
LOCUS       DASSWR010000168         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_459111,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000168 DASSWR010000000
VERSION     DASSWR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459111"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..182)
                     /locus_tag="VFO47_02765"
     tRNA            complement(110..182)
                     /locus_tag="VFO47_02765"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Thr,seq:tgt)
     gene            complement(211..1164)
                     /gene="rlmB"
                     /locus_tag="VFO47_02770"
     CDS             complement(211..1164)
                     /gene="rlmB"
                     /locus_tag="VFO47_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET8787656.1"
                     /translation="MAPPPIGGRRRRDGDPRPAKARRDRDGGGEVRRPQAPSEKRRGG
                     SGARKAKQSRRRDQLDAAREAARPGQGRTLIEGRRPVVEALRAGRPMDRILLASGAGR
                     AALGDLLDLAERRGVEVQTVPRSLLDAEARSGAHQGVLAVVAPIQPIDLTELLAVPLA
                     GREPPFFLALDGVEDPHNLGALARSAEAAGCHGLIVPRHRSAPLSAVAVKSSAGALEH
                     LPVAEVPNLARAIEQLRASGIWCIGLDGTADASLFALDLADEPVCIVVGSEGSGLHRL
                     VRDSCDALVHIPMSGHIESLNASVAGALALFEVRRRRHPAP"
     gene            complement(1146..2573)
                     /gene="cysS"
                     /locus_tag="VFO47_02775"
     CDS             complement(1146..2573)
                     /gene="cysS"
                     /locus_tag="VFO47_02775"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET8787657.1"
                     /translation="MALQLHNTLTRRKEPFTPRDHGRVGIYVCGPTVYGEPHIGNLRP
                     VVVFDVLRRHLTASGHQVLLVRNYTDVDDKIINAAGHDPLQAFVVAEHWGRVYDEITA
                     ALGVLPPDIAPRASAHIPEMLALITRLVDGGLAYQAGTDVYFAVGKWPEYGKLSHRDL
                     GELQAGARVEVNPDKRDPLDFALWKGAKPGEPTWGSPWGPGRPGWHIECSAMAGKYLG
                     TGFDIHGGGEDLIFPHHENEIAQSEGATGEPFARYWLHNAFLELKGEKMAKSVGNVVS
                     PRELLRRHRGVVLRYALLGAHYRSPLEFSEEVLADAAASYDRLATFATNAARALALAG
                     GDGADADAGGPQGVGGWRDRFQAALDDDLNVPAALAVLFDLVAEANPLIVRLERGDHD
                     ATGPLRDRFATFLDLAGRLGFTPLDDLPDPAAFRPLLDLLLDLRERARAARDFAAADA
                     IRDRLADAGIRVEDRPGGPRWHLLP"
     gene            complement(2616..>2953)
                     /locus_tag="VFO47_02780"
     CDS             complement(2616..>2953)
                     /locus_tag="VFO47_02780"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET8787658.1"
                     /translation="AAGLPDLGQHFPSVDPQWAGVSSIDLCARVAELVAASGWAPVNL
                     DVVVLCDRPRLGHLTATMAANLAAALGLEPARVRVTAKSTDGLGFCGRGEGIAATAVC
                     LAAEQPVRG"
BASE COUNT          366 a          930 c         1285 g          372 t
ORIGIN      
        1 cgcccgctcc cgttgccgcg gtcgcccatt ctggagctga agaatgaccg cggaatgacc
       61 gcgggacaag caaagcaagt gctacagggc tgtgacctgc gcttttggga gctggcgggg
      121 ggactcgaac ccccaacctg ctgtttacaa gacagctgcg ctgccgattg cgccacgcca
      181 gcgggcggtg ccgaaccaaa gggtacgtga tcagggggct gggtggcggc ggcggcggac
      241 ctcgaacagg gccagggcgc cggctaccga ggcgttgagg ctctcgatgt ggccggacat
      301 ggggatgtgg acgagggcgt cgcaggagtc gcggacgagg cggtggaggc cggagccttc
      361 gctgccgacg acgatgcaga cgggctcgtc ggcgaggtcg agggcgaaga ggctggcgtc
      421 ggcggtgccg tcgaggccga tgcaccagat gccggaggcg cgcagctgct cgatggcgcg
      481 ggcgaggttg gggacctcgg ccacggggag gtgctcgagg gcgccggcgg aggacttgac
      541 ggcgaccgcg gacagggggg cgctgcggtg gcgggggacg atcaggccgt ggcagccggc
      601 ggcttcggcg ctgcgggcca gggcgccgag gttgtggggg tcctcgacgc cgtcgagggc
      661 gaggaagaag gggggctcgc ggccggccag ggggacggcc aggagctcgg tcaggtcgat
      721 cggctggatg ggggccacga cggcgaggac gccctggtgg gcgccgctgc gggcctcggc
      781 gtcgagtagc gagcggggga cggtctggac ctcgacgccg cggcgctcgg ccaggtcgag
      841 gaggtcgccg agggcggctc ggccggcgcc ggaggcgagc aggatccggt ccatggggcg
      901 gccggcgcgg agggcctcga cgaccgggcg gcggccctcg atcagggtgc ggccctggcc
      961 agggcgggcc gcctcgcggg ccgcgtcgag ctggtcgcgg cggcgggact gcttggcctt
     1021 gcgggcgccg gagccgccgc ggcgcttctc ggacggggcc tgggggcggc ggacctctcc
     1081 cccgccgtcg cggtcgcggc gggccttggc cgggcgtgga tcgccgtcgc gccgccggcg
     1141 gccgcctatg ggaggaggtg ccatcggggg ccgccggggc ggtcctcgac ccggatgccg
     1201 gcgtcggcga ggcggtcgcg gatggcgtcg gcggcggcga agtcgcgggc cgcgcgggcg
     1261 cgttcgcgga ggtcgaggag gaggtcgagc agcgggcgga aggccgccgg gtcgggcagg
     1321 tcgtcgagcg gggtgaagcc gaggcggccg gccaggtcga ggaaggtggc gaagcggtcc
     1381 cggagcgggc cggtggcgtc gtggtcgccc cgctccagcc ggacgatcag cgggttggcc
     1441 tcggcgacca ggtcgaacag gacggccagg gcggcgggga cgttcaggtc gtcgtcgagg
     1501 gcggcctgga agcggtcccg ccacccgccc acgccctggg ggccgccggc atcggcgtcc
     1561 gcgccgtccc cgccggcgag ggcgagggcc cgggcggcgt tggtggcgaa cgtggccagg
     1621 cggtcgtagc tggcggcggc gtcggccagg acctcctcgg agaactccag ggggctgcgg
     1681 tagtgggcgc cgaggagggc gtagcggagg accaccccgc ggtggcggcg caggagctcg
     1741 cgggggctga ccacgttgcc gacggacttg gccatcttct cgcccttgag ctcgaggaag
     1801 gcgttgtgga gccagtagcg ggcgaacggc tcgccggtgg cgccctcgga ctgggcgatc
     1861 tcgttctcgt ggtgggggaa gatcaggtcc tcgccgccgc cgtggatgtc gaagccggtg
     1921 ccgaggtact tgccggccat ggccgagcac tcgatgtgcc agccggggcg gcccgggccc
     1981 cagggcgagc cccaggtggg ctcgccgggc ttggcaccct tccacagggc gaagtcgagc
     2041 gggtcgcgct tgtccgggtt gacctcgacc cgggcgccgg cctggagctc gcccaggtcg
     2101 cggtgggaca gcttgccgta ctcgggccac ttgccgacgg cgaagtagac gtcggtgccg
     2161 gcctggtagg cgagcccgcc gtcgaccagc cgggtgatca gggccagcat ctctgggatg
     2221 tgggcgctgg ccctgggggc gatgtcgggc ggcagcacgc cgagggcggc ggtgatctcg
     2281 tcgtagaccc gcccccagtg ctcggcgacc acgaacgcct gcagcgggtc gtggccggcg
     2341 gcgttgatga tcttgtcgtc gacgtcggtg tagttccgga ccagcaggac ctggtggccg
     2401 gaggcggtca ggtggcggcg gagcacgtcg aacacgacca cgggccggag gttgccgatg
     2461 tggggctcgc cgtagacggt gggcccgcac acgtagatcc ccacccggcc gtggtcgcgg
     2521 ggcgtgaacg gctccttgcg gcgggtgagc gtgttgtgga gctggagggc cacggtggtt
     2581 cggctccggg ttccgatcgg cggtgagctc gtatcctacc cgcgaaccgg ctgctcagcg
     2641 gccaggcaga cggcggtggc ggcgatcccc tcgccgcggc cgcagaagcc gaggccgtcg
     2701 gtggacttgg ccgtgacccg gacccgggcc ggctccaggc cgagggcggc ggccaggttg
     2761 gcggccatgg tggccgtgag gtggccgagg cggggccggt cgcagagcac gaccacgtcc
     2821 aggttgaccg gggcccagcc gctggccgcg accagctcgg ccacccgggc gcacaggtcg
     2881 atgctggaga cgcccgccca ttgggggtcg accgagggga agtgctggcc caggtcgggc
     2941 aggccggccg cgc
//
LOCUS       DASSWR010000169         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_233201,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000169 DASSWR010000000
VERSION     DASSWR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233201"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..733
                     /gene="msrA"
                     /locus_tag="VFO47_02785"
     CDS             215..733
                     /gene="msrA"
                     /locus_tag="VFO47_02785"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET8787659.1"
                     /translation="MSQKREKAVLAGGCFWGMQDLIRKRPGVLSTRVGYAGGDVANAT
                     YRNHGTHAEAIEIIFDPEVTSYRELLEFFFQIHDPTTLNRQGNDIGTSYRSAIFYLDD
                     EQRRVAEDTIADVEASGLWPGKVVTDVTPAGPFWEAEPEHQDYLERYPNGYTCHFPRP
                     GWVLPRRSAAAR"
     gene            complement(888..>2765)
                     /gene="ftsH"
                     /locus_tag="VFO47_02790"
     CDS             complement(888..>2765)
                     /gene="ftsH"
                     /locus_tag="VFO47_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET8787660.1"
                     /translation="GPRFWWILLGLLVLNWVLSTMLVRPPERTEVPYTLFRAQVEAGN
                     VASVTGIEDAIDGNFKNPVRVPVDDQTQEVTRFSTRRPSFAADDNLWELLTEHNVEVS
                     AEPPPGPSLLERVLLGFGPTLLLVGLFVLLARRAAGGAGGAGALGGFGRSRARRYEPE
                     TGRRTTFADVAGIDEVEDEVAEIVDFLKNPDRYRRLGAMVPKGVLLAGPPGTGKTLLA
                     RAVAGEADVPFYFVSASEFIEFIVGVGASRVRDLFETAKNNAPAIIFIDELDAIGRAR
                     GGSLSVGGHDEREQTLNQILTEMDGFTGTESVIVLGATNRPEILDPALLRPGRFDRRL
                     TISPPDQAGRRQILGVHVRGMPLAPDVDLDAVASATPGMVGADLANLVNEAAILAARR
                     NHTQVQQADFTDALEKIVLGSERRIMLSAEDRRRTAYHEAGHALLGMLTPGADPVRKI
                     SIVPRGHALGVTFQSPDADRYSYSASYLRGRIAGMLAGRAAEELIYDDVTTGAENDLE
                     QATGIAKQMVGRWGMSEKVGMLSALPDPRREQPLSLGGDGTSPATRELVETEARRILD
                     QGHDQALQTLRANRHRLDALVEALLRTETLDAADAYRAAGVPYPAQPDLPAQAPDVVR
                     R"
BASE COUNT          388 a          938 c         1051 g          388 t
ORIGIN      
        1 gcagcgcgcc ctgagccgtt catgtgcatt cccggctgac cggctcacga gctcagccgg
       61 ccgcatcggc gacttgggcg ctgagtcttg gcctgggaat gcgccaaggt gtcggagcgt
      121 taccggctgc agtgcaaacc cgaggttggg tgctggagtt aggggcgtgc cggaacctcc
      181 gcaatcaagc cgcgacgacg aacggagcga agcgatgagc cagaagcggg agaaggcggt
      241 cctggccgga gggtgtttct gggggatgca ggacctgatc cgcaagcgtc caggggtcct
      301 gtcgacgcgg gtcggctacg ccggaggcga cgtcgccaac gccacctacc ggaaccacgg
      361 gacccatgcc gaggcgatcg agatcatctt cgaccccgag gtgacctcct accgcgaact
      421 gctcgagttc ttcttccaga tccacgaccc gacgacgctg aaccgccagg gcaacgacat
      481 cggcacgagc taccgctcgg cgatcttcta cctcgatgac gaacagcgcc gggtcgccga
      541 ggacaccatc gccgacgtcg aggcgtccgg gctctggccc ggcaaggtcg tcaccgacgt
      601 caccccagcc ggtccgttct gggaggccga gccggagcac caggactacc tggagcgcta
      661 cccgaacggc tacacctgcc acttcccgcg cccgggctgg gtgctgcccc gccggagcgc
      721 cgcggctcgc tagcagacgg tctgcgcgca cgctcctgcg cgagtacatc gagcagggcg
      781 ggctgggcgt caagcgcggc cgcgggttct acgacgagcc cggctgagcc gacggtgagc
      841 gcccaccatg gctgctgggg cgccgacctg ctggctgccg gcggacgcta gcggcggacc
      901 acgtcggggg cctgggcggg caggtcgggc tgggccgggt aggggacgcc ggcggcgcgg
      961 taggcgtcgg cggcgtcgag ggtctcggtg cggagcaggg cctcgaccag ggcgtcgagc
     1021 cggtgccggt tggcccgcag ggtctggagg gcctggtcgt ggccctggtc gaggatgcgg
     1081 cgggcctcgg tttcgaccag ctctctggtc gccggggagg tgccgtcgcc gcccaggctg
     1141 aggggctgct cgcgccgcgg gtcgggcagc gccgacagca tccccacctt ctcggacatg
     1201 ccccagcgcc cgaccatctg cttggcgatg ccggtggcct gctcgaggtc gttctcggcg
     1261 ccggtggtga cgtcgtcgta gatgagttcc tcggcggccc ggccggccag catcccggcg
     1321 atgcggccgc gcaggtagct ggcgctgtag gagtagcggt cggcgtcggg ggactggaag
     1381 gtgaccccga gggcgtggcc gcggggcacg atcgagatct tgcggaccgg gtcggcgccc
     1441 ggggtcagca tgccgagcag ggcgtggccg gcctcgtggt aggcggtgcg ccgacggtcc
     1501 tcggccgaga gcatgatccg ccgttcgctg ccgagcacga tcttctccag ggcgtcggtg
     1561 aagtcggcct gctgcacctg ggtgtggttg cggcgggcgg ccaggatggc ggcctcgttg
     1621 accaggttgg ccaggtcggc cccgaccatg cccggggtgg ccgaggccac cgcgtccagg
     1681 tcgacatcgg gcgccagggg catgccgcgg acgtgcacgc ccaggatctg gcggcggccg
     1741 gcctggtcgg gcgggctgat ggtgagccgg cggtcgaagc gccccgggcg cagcagcgcc
     1801 gggtcgagga tctcgggccg gttggtggcg cccagcacga tcacactctc ggtgccggtg
     1861 aagccgtcca tctcggtcag gatctggttg agggtctgct cccgctcgtc gtggccgccc
     1921 acgctgaggg acccgccgcg ggcacggccg atcgcgtcca gctcgtcgat gaagatgatc
     1981 gccggggcgt tgttcttggc cgtctcgaac aggtcccgga cgcggctggc gcccacgccc
     2041 acgatgaact cgatgaactc cgaggccgac acgaagtaga agggcacgtc ggcctcgccg
     2101 gccacggccc gggcgagcag ggtcttgccg gtcccgggcg gcccggccag cagcaccccc
     2161 ttggggacca tggcccccag ccgccggtag cggtcagggt tcttgaggaa gtcgacgatc
     2221 tcggccacct cgtcctcgac ctcgtcgatg ccggccacat ccgcgaaggt ggtgcgccgg
     2281 ccggtctcgg gctcgtagcg gcgggcccgg gagcggccaa agccgccgag ggcgcccgcg
     2341 cccccagccc ctcccgccgc ccgccgggcc agcagcacga acaggccgac caggagcagg
     2401 gtggggccga agccgagcag cacccgctcc agcagcgacg gccccggcgg cggctcggcc
     2461 gagacctcca cgttgtgctc tgtcagcagc tcccagaggt tgtcgtcggc ggcgaaggag
     2521 ggccggcggg tgctgaaccg ggtcacctcc tgggtctggt cgtcaacggg gacgcggacc
     2581 gggttcttga agttgccgtc gatggcgtcc tcgatcccgg tgacggaggc gacgttgccc
     2641 gcctcgacct gggcgcggaa caacgtgtag ggcacctcgg tgcgttcggg gggccggacc
     2701 agcatggtcg acagcaccca gttgagcacc agcaggccga gcaggatcca ccagaagcgg
     2761 ggccc
//
LOCUS       DASSWR010000170         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_261584,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000170 DASSWR010000000
VERSION     DASSWR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261584"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..750
                     /locus_tag="VFO47_02795"
     CDS             154..750
                     /locus_tag="VFO47_02795"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET8787661.1"
                     /translation="MFTGIVEGTGTVAALAVADGGGGARLEVEAPFLAGDLRLGESVA
                     VNGCCLTVAEPTPGGFAADLVAETLRRTALGGLAAGDAVNLERPLAVGGRLGGHLVQG
                     HVDGVARVLERKPVGEGEEVRVELPPDLERYVVEKGSIAVDGVSLTVAGVGPGWFAVA
                     LVPHTLEVTTLGRRRPGDPVQLEVDVVAKYVERLVVRQ"
     gene            747..2015
                     /locus_tag="VFO47_02800"
     CDS             747..2015
                     /locus_tag="VFO47_02800"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET8787662.1"
                     /translation="MSGFASVEDAVAALAAGGMIVVVDDADRENEGDLIMAAEAATPE
                     TIGFFVRHTSGVICTPMLGERLDELDLPLMVAVNTAPYRTAFTVSVDARAGTTTGISA
                     ADRARTIQALIDPATKPADLVRPGHIFPLRYREGGVLKRAGHTEAAVDLARMAGRTPA
                     AVLCEIVDDDGEMARLPQLERFADAHGLPLISIADLIRSRRQHEKLVRRVAEARIPTR
                     WGDFTAYAYESLLDGEQHLALVKGAVQGEENVLVRVHSECLTGDALGSLRCDCGPQLE
                     DALARIADEGLGVVVYLRGHEGRGIGLGHKLRAYHLQEQGRDTVDANLDLGLPVDGRE
                     YGIGSQILVDLGVTTMRLLTNNPAKYGGLDGFGLQIVERVPLQTRPNPENLRYLTTKR
                     DRMGHLLELPTEGGSGGLEPGPPENGEKVT"
BASE COUNT          279 a          796 c          810 g          269 t
ORIGIN      
        1 gctggccatc ggcggggcgg gcgcccctgg gctgctgggc ggggccggag cggccaccct
       61 ggccgaggcc cgccggctcc ggctggcctc ggtcgaccgg ctgggcgacg acgttcgcgt
      121 ggtggcgttt ccatgcgccg aggaggagga ctgatgttca ccgggatcgt ggaaggcacc
      181 ggcaccgtgg ccgcgctggc cgtcgcggac ggcggcggcg gggcccggct ggaggtcgag
      241 gcgccgttcc tggccggcga cctgcggctg ggagagtcgg tggccgtcaa cggctgctgc
      301 ctgaccgtgg ccgagccgac ccccggcggg ttcgcggccg acctggtcgc cgagaccctg
      361 cgccgcaccg cccttggcgg gctggccgcc ggggacgcgg tcaacctgga gcggcctctg
      421 gccgtgggcg gccgcctcgg cggccacctc gtccagggcc acgtcgacgg ggtggccagg
      481 gtgctggagc gcaagccggt cggtgagggc gaggaggtca gggtcgagct gccgcccgac
      541 ctggagcgct acgtggtcga gaaggggtcg atcgcggtcg acggggtcag cctgaccgtg
      601 gccggggtcg ggcccgggtg gttcgcggtc gcgctggtgc cccacaccct ggaggtgacc
      661 accctgggcc ggcgccgccc cggcgacccg gtgcagctgg aggtcgacgt ggtcgccaag
      721 tacgtcgagc ggctggtggt gcgccaatga gcggcttcgc cagcgtcgag gacgccgtgg
      781 ccgcgctcgc cgccggcggc atgatcgtgg tcgtcgacga cgccgaccgc gagaacgagg
      841 gcgacctgat catggccgcc gaggcggcca ccccggagac gatcggcttc ttcgtccgcc
      901 acacctccgg ggtcatctgc acccccatgc tcggtgaacg gctcgacgag ctcgacctgc
      961 ccctgatggt ggcggtcaac accgccccct accggaccgc cttcaccgtc tcggtcgacg
     1021 cccgcgccgg caccaccacc ggcatctcgg ccgccgaccg ggcccggacc atccaggccc
     1081 tgatcgaccc cgcgaccaag cccgccgacc tggtccggcc cggccacatc ttccccctcc
     1141 gctaccgcga gggcggggtc ctcaagcgcg ccggccacac cgaggccgcg gtcgacctgg
     1201 cccgcatggc cggccggacc ccggcggccg tgctctgcga gatcgtcgac gacgacggcg
     1261 agatggcccg cctgccccag ctggagcgct tcgccgacgc ccacggcctg ccactcatct
     1321 ccatcgccga cctgatccgg tcccggcgcc agcacgagaa gctggtcagg cgggtcgccg
     1381 aggcccgcat cccgacccgc tggggcgact tcaccgccta cgcctatgag tcgctgctcg
     1441 acggcgagca gcacctggcc ctggtcaagg gggccgtcca gggcgaggag aacgtgctcg
     1501 tgcgggtcca ctccgagtgc ctcaccggcg acgccctggg cagcctgcgc tgcgactgcg
     1561 gcccccagct ggaggacgcc ctggcccgga tcgccgacga gggcctcggg gtggtcgtgt
     1621 acctgcgcgg ccacgagggc cgcggcatcg ggctcggcca caagctgcgt gcctaccacc
     1681 tccaggagca gggccgcgac accgtcgacg ccaacctcga cctgggcctc ccggtcgacg
     1741 ggcgcgagta cggcatcggc tcccagatcc tggtcgacct gggggtgacg accatgcggc
     1801 tgctcaccaa caacccggcc aagtacggcg ggctggacgg cttcggcctc cagatcgtgg
     1861 agcgggtgcc cctccagacc cgccccaacc ccgagaacct gcgctatctc acaaccaagc
     1921 gcgaccgcat gggccacctg ctggagctgc ccacggaggg ggggtccggg gggctggagc
     1981 cgggccctcc ggagaacggg gagaaggtga catgagccgg gtctacgagg gttccatcga
     2041 cgggcggggc cggcggatcg cggtggccgc cagccggttc aaccggctgg tcaccgaccc
     2101 gctggtcgcc ggggcggtcg aggagctgcg gcgccacggg gtcgccgagg agga
//
LOCUS       DASSWR010000171         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1222571, whole genome shotgun sequence.
ACCESSION   DASSWR010000171 DASSWR010000000
VERSION     DASSWR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222571"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..972
                     /locus_tag="VFO47_02805"
     CDS             13..972
                     /locus_tag="VFO47_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET8787663.1"
                     /translation="MDPAAVRSAAARIAGHVHRTPVRTSATLDGRTGAQVLVKDEAVQ
                     KTGSFKARGALNRLLTLPDDARERGLVCVTAGNHGAAVAWAAAQVGAKATVVMPTYSP
                     AAKVAACWGYGAEVVLHGDTTGEAFAECERLQAERGLTFIHPFDDPEIVAGQGTVALE
                     LVEDAGPVDVWVVSVGGGGLTCGTALVVRDANPGCRVVAVEPEQAAALTAALAAGRPV
                     PVVPRSVADGLCAPFAGPLTFPLFRDLVDEVVLLTEAEILAGVRFVMERMKVVVEPAG
                     AATVAALLAGKAGDLTGRRVGTVLTGGNVDLGVVVPKPPAPDA"
     gene            1042..1308
                     /locus_tag="VFO47_02810"
                     /pseudo
     CDS             1042..1308
                     /locus_tag="VFO47_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            1372..1797
                     /locus_tag="VFO47_02815"
     CDS             1372..1797
                     /locus_tag="VFO47_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET8787664.1"
                     /translation="MTATLDVEQEVVVAAPREKVFDALCRMGEWWPHHFREGSTVHLE
                     PRVGGRFWEDWGDGDGALYATVTGIRRPEQLTCTGPMGMRTPVTGVFTMALEEQPDGT
                     LVRLSHHAVGPIDSETLAAYQTGWGEVFDALRGHLGLAG"
     gene            1912..>2825
                     /locus_tag="VFO47_02820"
     CDS             1912..>2825
                     /locus_tag="VFO47_02820"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET8787665.1"
                     /translation="MRLTPLLDRWNDDPAFAELLAALAQGQDQVRSEVVVPDVARAFL
                     LAGLAQAGGRLVVVTTATTADAEALAADVAAFCGPDSAAVFPAWETLPHERLSPRSET
                     VAARLRLLHRLGAGDTDGLRLLAVPARAMMQPLAPGLDAVDPVRVARGDRVDLEELLE
                     RLVAAGYQRTDMVERRGEVAVRGGLVDFFPPGEDHPVRVELWGDEVDSVRSFAVSSQR
                     SLTELPEVEAWPCREVRLGEAERGRARQLAAEVMGTQDLLAQVAEGLDVEGVESLLPL
                     LFDRLQPLPAYLPGDAVLVLVDPKRTLDR"
BASE COUNT          339 a         1058 c         1056 g          372 t
ORIGIN      
        1 gaaggagcag acgtggaccc agcggccgtg cggtccgcag cggcgcggat cgccgggcac
       61 gtgcaccgca ccccggtacg gacctcggcc accctcgacg ggcgcaccgg cgcccaggtc
      121 ctggtcaagg acgaggcggt ccagaagacc ggctcgttca aggcccgcgg cgccctcaac
      181 cgcctcctca ccctgcccga cgacgcccgc gagcgggggc tggtgtgcgt caccgccggc
      241 aaccacgggg ccgccgtggc ctgggcggcc gcccaggtcg gggccaaggc gaccgtggtc
      301 atgccgacct acagcccggc ggccaaggtg gccgcctgct ggggctacgg cgccgaggtg
      361 gtgctgcacg gagacaccac cggggaggcc ttcgccgagt gcgagcggct ccaggccgag
      421 cgcggcctca ccttcatcca ccccttcgac gaccccgaga tcgtcgccgg ccagggcacc
      481 gtcgccctgg agctggtgga ggacgccggg ccggtcgacg tctgggtggt gagcgtcggc
      541 gggggcggcc tcacctgcgg caccgccctg gtcgtgcgtg acgccaaccc gggctgccgg
      601 gtggtcgcgg tcgagcccga gcaggcggcc gccctgaccg ccgccctggc cgccggccgg
      661 ccggtcccgg tcgtgccccg gtcggtggcc gacgggctct gcgccccgtt cgccgggccg
      721 ctcaccttcc ccctcttccg cgacctggtc gacgaggtcg tgctcctgac cgaggcggag
      781 atcctggccg gggtccgctt cgtgatggag cggatgaagg tcgtggtcga gccggccggg
      841 gccgcgaccg tcgccgccct gctggccggc aaggccggcg acctgaccgg ccggcgggtc
      901 ggcacggtcc tcaccggcgg caacgtcgac ctgggcgtgg tcgtgcccaa gccgccggca
      961 ccggacgctt gacggccgcc acctcatatg tgaccatctg gttacgtaac tagatggtca
     1021 ccaatggttc cgggagattc catggtcgac gacaccgctc cggtctggaa ggcgctggcc
     1081 gacccgaccc ggcgggccat cctcgacctg ctgcgggagc ggtcgcacac caccggcgag
     1141 ctgagcgggg ccttcccggc cctcacccgg ttcgcggtca tgaagcacct gggggtgctg
     1201 gaggaggccg ggctggtcgt cgtcaggcgc caggggcggg agcgctggaa ccacctcaac
     1261 ggggtgccgc tgcgggaggc ctacgagcgc tggatgcgac cgtacgccga ccgctgggcc
     1321 gagtcgctgc tccggctgaa ggagacggcc gagcggcagg aaggagctgg catgaccgcg
     1381 accctggacg tggagcagga ggtggtggtg gccgccccgc gggagaaggt gttcgacgcc
     1441 ctctgccgga tgggggagtg gtggccgcac cacttccggg agggctcgac cgtgcacctg
     1501 gagccacggg tgggcgggcg cttctgggag gactggggcg acggcgacgg cgccctgtac
     1561 gccaccgtga ccggcatccg ccggcccgag cagctgacct gcaccggccc catgggcatg
     1621 cgcaccccgg tgaccggcgt gttcaccatg gccctcgagg agcagcccga cggcaccctg
     1681 gtgcggctct cccaccacgc ggtcggcccg atcgattccg agaccctcgc cgcctaccag
     1741 accggctggg gcgaggtgtt cgacgccctc cgcggccacc tcggtctggc cggctgaccg
     1801 tcggcatgct gcggtagcct ggacatcgac ataacgggag cccccggctg cggccgtcgg
     1861 ggctacccgg ctgtgctgtg ctcaacgccg caaccgctgc aaaggatttc cgtgcgcctc
     1921 acccccctgc tcgaccgctg gaacgacgac cccgcctttg ccgagctgct ggccgccctt
     1981 gcccagggcc aggaccaggt gcgctccgag gtcgtcgttc ccgacgtggc cagggcgttc
     2041 ctgctcgccg ggctggccca ggccggcggc cggctggtgg tggtgaccac cgccaccacg
     2101 gccgacgccg aggccctggc cgccgacgtg gccgccttct gcggccccga ctcggcggcc
     2161 gtcttcccgg cctgggagac cctgccccac gagcggctct cgccccgctc cgagacggtg
     2221 gccgcccgcc tgcgcctgct ccaccggctc ggcgccggcg acacggacgg actccgcctg
     2281 ctggccgtgc cggcccgggc gatgatgcag ccgctggccc cggggctgga cgccgtcgac
     2341 ccggtgcggg tggcccgggg cgaccgggtc gacctcgagg agctgctgga gcggctggtc
     2401 gccgccggct accagcgcac cgacatggtc gagcgccggg gcgaggtcgc cgtccgcggc
     2461 ggcctggtcg acttcttccc gccgggtgag gaccatccgg tccgggtgga gctgtggggc
     2521 gacgaggtcg acagcgtccg ctccttcgcc gtgtccagcc agcggtccct gaccgagctg
     2581 cccgaggtcg aggcctggcc gtgccgcgag gtgcgcctgg gcgaggccga gcggggccgg
     2641 gcccgccagc tcgccgccga ggtcatgggc acccaggacc tgctcgccca ggtggccgag
     2701 ggcctggacg tcgagggggt cgagtcgctg ctgccgctgc tgttcgaccg gctccagccg
     2761 ctgccggcct acctccccgg cgacgccgtg ctggtcctgg tcgaccccaa gcgcaccctc
     2821 gaccg
//
LOCUS       DASSWR010000172         4061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_374523,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000172 DASSWR010000000
VERSION     DASSWR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374523"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..854)
                     /locus_tag="VFO47_02825"
     CDS             complement(12..854)
                     /locus_tag="VFO47_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit C"
                     /protein_id="HET8787666.1"
                     /translation="MTDVATPTVERPAASEPIDYKPDKPGRGPIDWMGGWRVCWMGSL
                     AFILLALAIRIFQQFTAWSVGIDASSRDFGLYYRSLFIAEVLGVTVGTLLWWGYLVRR
                     GRKVVLREATHAEEVRRIAVFWGLVGITSVILYIMASFWPNQDGSWHQTAVRDTALTP
                     SHIPMFFLFFPLGITITVGTYLYGRYWLPKVYGAEKGFPWSFFFLIAASVTEMAQVAM
                     NEWGHSLWITEEIFAVPFHWPFVFYGWLAAGIFALWAETIIRLLQIEGEIEAEAGQPA
                     EEVA"
     gene            complement(994..1725)
                     /locus_tag="VFO47_02830"
     CDS             complement(994..1725)
                     /locus_tag="VFO47_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET8787667.1"
                     /translation="MTEARTGGLVDSPPVDSSAVLEALGTVRDPELDEPLPDLGFVSS
                     LEVDGDTVRVRLRLPTYFCAPNFAYLMVADADTAIRAVPGVRQAEVFLDDHFTSEEIN
                     HGVAQRRGFQQSFDGLADDELDELRTLFRRKALINRQQRLVKALLDGGATTGELGRLR
                     LGDLPPSPELDTYLQRRAELGLDVGREAPLVVDTDGNPIPPELLERHLRFASLTRVSI
                     EGNAGFCRGMLATRYDLPTPTVVTD"
     gene            complement(1722..2777)
                     /locus_tag="VFO47_02835"
     CDS             complement(1722..2777)
                     /locus_tag="VFO47_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8787668.1"
                     /translation="MYEKDGEKYFVVDGHMHFWDASEENTKNKYGEGFIRCFYDYHKN
                     LSPEDYVWPLEKYRKYDEPTLMKDLFEDGYVDVAIFQPTYLTDFYHQGFNTTAQDAVL
                     KEKYPDKFVLNTSFEPRLGEAGLAMFEEKVRKYGSKGVKLYTAEWHDASKGWKLTDPW
                     ALRHLEKCEELGVTNIHVHKGPTIWPLNRDSFDLADVDDAASTFPGLNFIVEHVGLPR
                     LEDMCWIGTQEPNVYGGLAVAMPFIHTRPAYFGQVVGELLYWLGEDRLLFASDYALWH
                     PKWLVEKFVDYQYPMENEYPEITTNVKKKILGLNAAKLYDLKVPEGVSAGDEGTGQDV
                     TEKPAVPVEASVGGHSE"
     gene            complement(2790..3824)
                     /locus_tag="VFO47_02840"
     CDS             complement(2790..3824)
                     /locus_tag="VFO47_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET8787669.1"
                     /translation="MQALRLHRYNERPTVDEIEEPKVEDPLDVIVKIGGAGLCRTDIH
                     LYLGQWHELDADAPLPYTLGHENAGWVHEVGSAVSHVAPGDTVILHPQATCGFCRYCR
                     AGDDMHCVNSFFPGLNTHGGMATYLRTGARAVVKIDPKLQPADVAALADAGLTAYHAV
                     RKAVPLLYPGTACVVIGAGGLGHIAIQVLAALCATTIIVVDRSPEALEFAKTIGADHG
                     VVADGGHVDKVKELTGGNGAEVVLDFVGEEGAEKDAWQMTRRAGSHFVIGYGGIVEVP
                     TIEIISTERNVIGNIVGTYNDLAELMALAAQGKVTLHTKPYPLEAANDAIDDLDNGRL
                     PGTRAILIPS"
BASE COUNT          619 a         1303 c         1451 g          688 t
ORIGIN      
        1 cgggcggcct ctcaggcgac ctcttccgcg ggctgtccag cctcagcctc gatctcgccc
       61 tcgatctgga gcaggcggat gatcgtctcg gcccagaggg cgaagatccc ggccgccagc
      121 cagccgtaga agacgaacgg ccagtggaac gggacggcga agatctcctc ggtgatccag
      181 aggctgtggc cccactcgtt catggcgacc tgggccatct cggtgaccga ggcggcgatc
      241 aggaagaaga acgaccaggg gaagcccttc tcggccccgt acaccttggg cagccagtag
      301 cggccgtaga ggtaggtgcc gaccgtgatg gtgatcccca gggggaagaa caggaagaac
      361 atcgggatgt gggacggcgt gagggcggtg tcgcgcacgg ccgtctggtg ccaggagccg
      421 tcctggttgg gccagaagct ggccatgatg tacaggatca cgctggtgat cccgaccagc
      481 ccccagaaca cggcgatccg gcggacctcc tcggcgtggg tggcctcccg aagcaccacc
      541 ttgcgcccgc gccgcaccag gtagccccac cacagcaagg tgcccacggt cacgccgagg
      601 acttcggcga tgaacaggga gcggtagtag aggccgaagt cgcggctgga ggcgtcgatc
      661 ccgaccgacc aggcggtgaa ctgctggaag atgcggatcg ccagcgccag gaggatgaag
      721 gccaggctgc ccatccagca gacccgccag ccgcccatcc agtcgatcgg gcccctgccg
      781 ggcttatccg gcttgtagtc gatcggctca ctggccgccg ggcgctcaac ggtcggcgtt
      841 gctacgtccg tcatgcctgt tcctttctct ccaccgggac cggcactgac gtgttccaag
      901 cgtgtcttga aggtaagaag acgcggaggc cgtcacaagc tacgaacccc tataacctcg
      961 catcgttgca acaatgtgcg aattgacgac gaattagtcg gtgaccacgg tcggggtcgg
     1021 gaggtcgtag cgggtcgcga gcatgccccg gcagaagccg gcgttgccct cgatgctcac
     1081 gcgggtcagg ctggcgaagc gcaggtgccg ttcgagcagc tcgggtggga tggggttgcc
     1141 gtccgtgtcg accaccaggg gcgcctcccg gccgacgtcc agccccagct cggcccggcg
     1201 ctgcaggtag gtgtccagct ccggcgacgg gggcaggtcg ccgaggcgca ggcggcccag
     1261 ctccccggtg gtcgcgccac cgtccagcag ggccttgacg agccgctgct ggcggttgat
     1321 cagcgccttg cgccggaaca gggtgcgcag ctcgtcgagc tcgtcgtcgg ccaggccgtc
     1381 gaacgactgc tggaagccgc gccgctgggc gacgccgtgg ttgatctcct cggaggtgaa
     1441 gtggtcgtcg aggaagacct cggcctggcg gacgccgggc acggcccgga tggccgtgtc
     1501 ggcgtcggcc accatcaggt aggcgaagtt gggggcacag aagtaggtgg gcaggcgcag
     1561 gcggacccgg acggtgtccc cgtccacctc gagggacgac acgaacccga ggtccggaag
     1621 gggctcgtcc agctcggggt cacgcaccgt gccgagcgcc tccagcaccg cggagctgtc
     1681 gaccgggggg ctgtccacca gccccccggt ccgtgcctcg gtcactcgct gtgcccgccc
     1741 acgctggcct cgaccgggac cgccggcttc tcggtgacgt cctggccggt gccctcgtcg
     1801 ccggcgctga cgccctccgg gaccttgagg tcgtacagct tggccgcgtt cagcccgagg
     1861 atcttcttct tcacgttggt ggtgatctct gggtactcgt tctccatcgg gtactggtag
     1921 tcgacgaact tctcgaccag ccacttgggg tgccagaggg cgtagtcgct ggcgaacagc
     1981 aggcggtcct caccgagcca gtacagcagc tcgcccacga cctggccgaa gtaggcgggc
     2041 cgggtgtgga tgaacggcat cgccaccgcc aggccgccgt acacgttcgg ctcctgggtg
     2101 ccgatccagc acatgtcctc cagccggggc agcccgacgt gctcgacgat gaagttcagg
     2161 cccgggaagg tgctggcggc gtcgtcgaca tcggccaggt cgaaggagtc ccggttgagg
     2221 ggccagatgg tcgggccctt gtggacgtgg atgttggtga cgccgagctc ctcgcacttc
     2281 tcgaggtggc gcagggccca cgggtcggtg agcttccagc ccttggaagc gtcgtgccac
     2341 tcggccgtgt acagcttcac gcccttggag ccgtacttgc ggaccttctc ctcgaacatg
     2401 gccaggccgg cctcgcccag gcggggctcg aaggaggtgt tgaggacgaa cttgtccggg
     2461 tacttctcct tgaggaccgc gtcctgcgcg gtggtgttga acccctggtg gtagaagtcg
     2521 gtcaggtagg tcggctggaa gatggccacg tcgacgtagc cgtcctcgaa gaggtccttc
     2581 atcagggtgg gctcgtcgta cttgcggtac ttctcaaggg gccagacgta gtcctcgggg
     2641 ctcaggttct tgtggtagtc gtagaagcag cggatgaagc cctcgccgta cttgttcttg
     2701 gtgttctcct cgctcgcgtc ccagaagtgc atgtggccgt cgaccacgaa gtacttctcg
     2761 ccgtccttct cgtacatgag gcgcctccct tagctgggga tgaggatcgc gcgggtgccg
     2821 ggcagccggc cgttgtcgag gtcgtcgatg gcgtcgttgg ccgcctccag ggggtagggc
     2881 ttggtgtgca gggtgacctt gccctgggcg gccagggcca tcagctcggc aaggtcgttg
     2941 taggtgccga cgatgttgcc gatgacgttg cgctcggtgg agatgatctc gatggtcggg
     3001 acctcgacga tcccgccgta gccgatcacg aagtgcgacc cggcccgcct ggtcatctgc
     3061 caggcgtcct tctcggcgcc ttcctcgccg acgaagtcca gcaccacctc ggccccgttg
     3121 ccgccggtca gctccttgac cttgtcgacg tggccgccgt cggcgaccac gccgtggtcg
     3181 gctccgatgg tcttggcgaa ctcgagcgcc tcggggctgc ggtcgaccac gatgatggtg
     3241 gtggcgcaga gggcggccag cacctggatg gcgatgtggc cgaggccacc ggccccgatg
     3301 accacgcagg cggtgcccgg gtagagcagg ggcacggcct tgcggacggc gtggtaggcg
     3361 gtcaggccgg cgtcggccag ggcggccacg tcggccggct ggagcttggg gtcgatcttg
     3421 accacggccc gggccccggt ccggaggtag gtggccatgc ccccgtgggt gttgagcccg
     3481 gggaagaagc tgttgacgca gtgcatgtcg tccccggccc ggcagtagcg gcagaagccg
     3541 caggtcgcct gggggtggag gatgaccgtg tcgccggggg cgacgtggct gacggccgaa
     3601 ccgacctcgt ggacccagcc ggcgttctcg tggccgaggg tgtagggcag cggcgcgtcg
     3661 gcgtccagct cgtgccactg gcccaggtag aggtggatgt cggtccggca gaggccggcc
     3721 ccaccgatct tgacgatcac gtcgagggga tcctcgacct tcggctcctc gatctcgtcc
     3781 actgtgggcc gctcgttata gcggtggagc cgcagtgcct gcatcgatgc atcacctcgt
     3841 cctcggccga ggggggctgc catgacctct cgatcgttgg gtagatcgca gaaacgaaca
     3901 agtagccaaa atcccacgat agttgggaaa tcggtgggct ttgcgcgacg acgcggcagg
     3961 agacaatcga gtcgttgaac cttcgcgcgg ttcgtcccga tccccggcgc cgccgccccc
     4021 gggcggcggc ggttccctga ccatgggccg gatcctggcc c
//
LOCUS       DASSWR010000173         4898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1108860, whole genome shotgun sequence.
ACCESSION   DASSWR010000173 DASSWR010000000
VERSION     DASSWR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108860"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="VFO47_02845"
     CDS             complement(<1..2229)
                     /locus_tag="VFO47_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8787670.1"
                     /translation="MNSLLGRLGAFTVRRRWWVIGAWVVLLAVMGSFASGLTGRLGSG
                     GFEVPGSQSLAVQRDLEQRFANQFPTTALITVHDDTRTVDDPAFRAVVDGIATRVRAV
                     PNVGGVESFVSSGSPAFVSPDRRTTYLVAGLSGDQNTQLDTVPEVAAAAREGVPAGVE
                     VETGGGAAFYERMNEISRHDLEQAERVSFPITLVVLLLAFSSLVAAGLPIMLALVSLG
                     VTLGVLYFLAGVTDMNVYVTNTASIVGIGVGIDYALFVVTRFREELRRGRSVAEAVPV
                     TLATSGRAVALSGATVIVALAGMFLVDIQAFRSMAIGSMSVVAVAVLAAITLLPAVLS
                     LVGHWVDRLRIPMLRPRAAEGEGFWHRWAVRIMRRPWAFMAAALVVLVVLAVPFAGIR
                     LGQPGASILPADESPRLATERLAAEFGAGVTGPMEILVDTPGGAGTRANLERVDRLTR
                     VLQGDGAVAGVQSLTSVLPRADLDAYTRLYAGGLNGVDPELAPAVAGLANWDRGADLA
                     RITVVTREEPESEAAEGLLDRVRDELVPAAGLAGQARVGGATALNVDLAREISGQLPV
                     VVLSVLALSFVLLAMAFRSLVLPLQAIAMNLLSVGAAYGLIVAVFQWGWGEQLLGFTS
                     EGHIEVFVPLFLFSILFGLSMDYEVFLLSRIREEYLRTGDNQLAVARGLESTARTITS
                     AALVMVTVFAAFAAGRLVPFKEMGFGLAVAVLIDATIVRTILVPAVMRLVGRWNWWMP
                     AWL"
     gene            complement(2312..2926)
                     /locus_tag="VFO47_02850"
     CDS             complement(2312..2926)
                     /locus_tag="VFO47_02850"
                     /inference="COORDINATES: protein motif:HMM:NF041196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="HET8787671.1"
                     /translation="MTLAPTATDRGGETRRRILEVAAEAFADRGYAGTSLNDVLKASG
                     VTKGGFYFHFPSKEALALATLRQKQEQWAGVVMAAVMRRSRAVEQLDAMVEALCDLHE
                     QDPACRAISRLCTELGDEHPGLRPELGTQLTMWMDMVASILRKGQDEGDIRPDVEPTV
                     AAEIAVESFIGLETVSEALTARSDLRRRARDFRDLWFAAIRQPS"
     gene            complement(2982..4241)
                     /locus_tag="VFO47_02855"
     CDS             complement(2982..4241)
                     /locus_tag="VFO47_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787672.1"
                     /translation="MNPEPRSVQRVYLVLMLFNTLAASFIWGINTLFLLDAGLSATAA
                     FTANALFTAGMVLFEVPTGVVADTWGRRASYLLGSVSLAISTALYWLAWQVEAPFWAW
                     ATTSVLLGLGFTFFSGATEAWLVDALKFTGFKGNLESVFAKGQIVAGAAMLSGSVLGG
                     LVAQWTNLGVPYILRALALVVTFVVAFVFMRDWGFVPKPGKHPVREMGQVLRGSVHFG
                     LGNPPVRWMMLAAPFSFGVGIYAFYAMQPYLLELYGDEQAYSIAGLAAAIVAGAQIIG
                     GMAAPRVRLLFRRRTSAILGGLVLEGGLLVLLGLTTAFWVAVGLLVLWGLTAAATLPI
                     RQAYMNGLIPSEQRATVLSFDNLLGSSGGVVIQPALGKVADVWSYSTSYLVAAGVHLM
                     GVPFVALARRQRASADPVEVEPAQQGG"
     gene            complement(4295..4561)
                     /locus_tag="VFO47_02860"
     CDS             complement(4295..4561)
                     /locus_tag="VFO47_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET8787673.1"
                     /translation="MCRNITTLRGLEPPATSEEIEAAARQYVRKVSGVTRPSPAAEEA
                     FEQAVTAVARATAEVLAELPPRRQPPKADPPLRRPEVRARMAGR"
     gene            4692..>4898
                     /locus_tag="VFO47_02865"
     CDS             4692..>4898
                     /locus_tag="VFO47_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787674.1"
                     /translation="MKSVPLGRQVSAVVALLTPVAVLLVLLVVLIQRPLMLLAAAVCF
                     STGVAAAAYAVTRSGIRRTLAAVLA"
BASE COUNT          763 a         1732 c         1811 g          592 t
ORIGIN      
        1 gagccaggcc ggcatccacc agttccagcg cccgaccagc cgcatcacgg ccggcacgag
       61 gatggtccgg acgatggtgg cgtcgatcag gacggccacg gccagcccga agcccatctc
      121 cttgaagggc accagccggc cggccgcgaa ggccgcgaac accgtcacca tgaccagcgc
      181 cgccgaggtg atggtgcggg ccgtgctctc caggccgcgg gccacggcca gctggttgtc
      241 gccggtgcgc aggtactcct cgcggatccg ggagagcagg aacacctcgt agtccatgga
      301 cagcccgaac aggatcgaga acaggaacag gggcacgaag acctcgatgt ggccctcgga
      361 ggtgaagccg agcagctgct cgccccagcc ccactggaag acggccacga tcagcccgta
      421 ggcggcgccc accgacagca ggttcatggc gatcgcctgc agcggcagca ccagcgagcg
      481 gaaggccatg gccagcagga cgaacgacag ggccaggacg gagagaacga ccaccgggag
      541 ctggccggag atctcgcggg ccaggtcgac gttgagcgcg gtggccccgc cgaccctggc
      601 ctgcccggcc aggccggcgg ccgggacgag ctcgtcgcgg acccggtcga gcagcccctc
      661 ggccgcctcg gactcgggct cctccctggt cacgaccgtg atgcgggcca ggtcggcgcc
      721 ccggtcccag ttggccagcc cggcaacggc cggggccagc tcggggtcga cgccgttcag
      781 gccgcccgcg tacagccggg tgtaggcgtc caggtcggcc cggggcagga ccgaggtgag
      841 gctctgcacg ccggccacgg ccccgtcgcc ctggaggacc cgggtgagcc ggtccacccg
      901 ctccaggttg gccctggtac cggccccgcc cggggtgtcg acgaggatct ccatgggacc
      961 ggtgacgccg gccccgaact cggccgccag gcgctcggtg gccagccgcg ggctctcgtc
     1021 ggcgggcagg atgctggcgc cgggctggcc caggcggatg ccggcgaagg gcacggccag
     1081 gacgaccagc acgaccaggg cggcggccat gaacgcccac gggcggcgca tgatccggac
     1141 cgcccagcgg tgccagaagc cctcgccctc ggccgcccgg gggcgcagca tcgggatgcg
     1201 gaggcggtcc acccagtggc cgaccaggct gagcaccgcc ggcagcaggg tgatggcggc
     1261 cagcacggcc accgcgacca cgctcatcga cccgatggcc atggagcgga acgcctggat
     1321 gtcgaccagg aacatcccgg ccagggcgac gatcacggtg gccccggaca gggccacggc
     1381 ccggccggag gtggccaggg tgaccgggac ggcctcggcc accgaccggc cccggcgcag
     1441 ctcctcgcgg aagcgggtga ccacgaacag ggcgtagtcg atgccgacgc cgatgccgac
     1501 gatggaggcg gtgttggtga cgtagacgtt catgtcggtg acgccggcca ggaagtacag
     1561 gacgccgagg gtcacgccca ggctgaccag ggcgagcatg atcggcaggc cggcggcgac
     1621 caggctggag aacgccagca gcagcacgac cagggtgatc gggaagctga cccgctcggc
     1681 ctgctcgagg tcgtggcggg agatctcgtt catgcgctcg tagaacgcgg cacccccacc
     1741 ggtctcgacc tcgaccccgg ccgggacgcc ctcgcgggcc gccgccgcga cctcggggac
     1801 ggtgtcgagc tgggtgttct ggtcgccgga gaggccggcg accagatagg tggtgcgccg
     1861 gtcgggcgag acgaaggccg ggctgccgct ggagacgaac gactccaccc cgccgacgtt
     1921 ggggaccgcc cgcacccggg tggcgatgcc gtcgacgacc gcccggaagg cggggtcgtc
     1981 gaccgtccgg gtgtcgtcgt ggacggtgat cagggcggtg gtcgggaact ggttggcgaa
     2041 gcgctgctcc aggtcgcgct ggacggccag cgactgcgat ccggggacct cgaacccgcc
     2101 agagccgagc cggccggtca gccccgaggc gaagcttccc atgacggcca gcagcaccac
     2161 ccaggcgccg atgacccacc agcggcgccg gacggtgaag gcaccgagtc gtcccagcag
     2221 gctgttcatc gttcctcccc cgtccggtcg aaaccgaccg gttggtatat tccgtgctcg
     2281 tacgcgttca cgggaaatgg cggcatggcg gtcaggatgg ctggcggatg gccgcgaacc
     2341 acaggtcgcg gaagtcccgg gcccggcggc gcaggtcgga ccgggcggtc agggcctcgg
     2401 agacggtctc caggccgatg aaggactcga cggcgatctc ggccgcgacc gtcggctcga
     2461 cgtcgggccg gatgtcgccc tcgtcctggc ccttgcggag gatcgaggcg accatgtcca
     2521 tccacatggt gagctgggtg cccagctcag ggcgcagccc ggggtgctcg tcgcccagct
     2581 cggtgcagag ccggctgatg gcccggcagg cggggtcctg ctcgtgcagg tcgcagaggg
     2641 cctcgaccat ggcgtcgagc tgctcgacgg cgcgcgagcg gcgcatcacg gccgccatca
     2701 ccacgcccgc ccactgctcc tgcttctggc ggagggtggc cagggccagc gcctccttgg
     2761 acgggaagtg gaagtagaag ccgcccttgg tgaccccgct ggccttgagg acgtcgttca
     2821 gggaggtgcc ggcgtagccc cggtcggcga acgcctcggc ggcgacctcg aggatgcggc
     2881 ggcgggtctc cccgccccgg tccgtggccg tcggcgccag cgtcatgccg ttcctccaga
     2941 ggtcgcgttc cgccggagat taaaaccgac cggtcggtct gtcaaccccc ttgctgggcc
     3001 ggctcgacct cgaccgggtc cgcgctcgcc cgctgccggc gggccagcgc cacaaatggc
     3061 acccccatca ggtgcacgcc ggccgcgacc aggtaggagg tggagtagct ccagacgtcg
     3121 gccaccttgc ccagcgccgg ctggatcacc acgccgccgc tcgacccgag caggttgtcg
     3181 aaggacagga cggtggcccg ctgctcggac gggatcaggc cgttcatgta ggcctgccgg
     3241 atcggcaggg tggcggcggc ggtcaatccc cacagcacca gcaacccgac cgcgacccag
     3301 aacgccgtcg tgagcccgag caggaccagc aggccgccct cgagcacaag gccgcccagg
     3361 atggccgagg tccgccggcg gaacagcagc cggacccgcg gcgccgccat gccgccgatg
     3421 atctgcgcgc cggcgacgat ggccgcggcc aggccggcga tgctgtaggc ctgctcgtcg
     3481 ccgtagagct ccagcaggta cggctgcatg gcgtagaagg cgtagatgcc gacgccgaag
     3541 ctgaacggcg ccgccagcat catccagcgg accggcgggt tccccaggcc gaaatgcacc
     3601 gagccgcgca gcacctggcc catctcgcgc accggatgct tgcccggctt ggggacgaag
     3661 ccccagtccc gcatgaagac gaaggcgacc acgaaggtca cgaccagggc cagcgcccgg
     3721 aggatgtagg gcacccccag gttggtccac tgggcgacca gcccgcccag gaccgagccg
     3781 gacagcatgg ccgccccggc gacgatctgg cccttggcga acaccgactc gaggttgccc
     3841 ttgaagccgg tgaacttgag ggcgtcgacc agccaggcct cggtcgcccc ggagaagaag
     3901 gtgaagccca gcccgagcag gaccgaggtg gtcgcccagg cccagaacgg ggcctccacc
     3961 tgccaggcca gccagtacag ggcggtggag atggccaggc tgaccgagcc gaggaggtag
     4021 gaggcgcggc ggccccaggt gtcggccacc accccggtcg gcacctcgaa caggaccatg
     4081 ccggccgtga acagggcatt ggcggtgaac gcggccgtgg cgctgagccc ggcgtccagc
     4141 aggaacagcg tgttgatgcc ccagatgaac gaggcggcca gggtgttgaa cagcatgagc
     4201 acgaggtaga cgcgctggac gcttcgcggc tcagggttca tggtccgctg aacctaccgc
     4261 cggccccgga cattccagaa ccgggggatc cgcctcagcg accggccatg cgggcgcgca
     4321 cctcggggcg gcgcagcggc gggtcagcct tcgggggctg gcgccgcggg ggcagctcgg
     4381 cgagcacctc ggccgtggcc cgggccacgg cggtcaccgc ctgctcgaac gcctcctcgg
     4441 cggccgggct ggggcgggtc acgccgctca ccttgcggac gtactggcgg gccgcggcct
     4501 cgatctcctc gctggtggcg ggcggctcca gcccccggag ggtggtgatg ttgcggcaca
     4561 tggtccccgt ccctctccct gtcctcctcg gattcgtcga ccgcaacgag cctaccgggc
     4621 ccctggaacg acggcttgtc ctgctcgcca cgacccggcc aagatgcaac ggtcggcaga
     4681 ggagggcgca gatgaagtcg gtacccctgg gacgccaggt gagcgcggtg gtggcgctcc
     4741 tcactccggt cgccgtgctc ctggtgctgc tggtcgtgct gatccagcgg ccgctgatgc
     4801 tgctggcggc ggccgtgtgc ttcagcaccg gggtggccgc ggccgcctac gccgtcaccc
     4861 ggtccgggat ccgccgcact ctggcggcgg tgctggcg
//
LOCUS       DASSWR010000174         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_374589,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000174 DASSWR010000000
VERSION     DASSWR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374589"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFO47_02870"
     CDS             <1..610
                     /locus_tag="VFO47_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005238367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET8787675.1"
                     /translation="DMSARIALHRGAGRVIGVDLVPERLERARARGVETLDLNDTDDL
                     VETVRDLTAGRGPDAVIDAVGMEAHGSPVGKLAHQMASLLPDALAAKMIETAGVDRLN
                     AFHLAIELVRRGGTISLSGVYGGMADPLPMLTLFDKQLQLRMGQANVRRWVGDILPLL
                     TDDDPLGVDTFATHHLPLADAPRGYEIFQKKQDGAVKILLQP"
     gene            642..1412
                     /locus_tag="VFO47_02875"
     CDS             642..1412
                     /locus_tag="VFO47_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8787676.1"
                     /translation="MHGADVVFLLVAGVLAGVAGSVAGLASLVSYPALLAVGLPALTA
                     NVTNTVALVGYTVSAAAFSRQELAGQAGRVRRLAGVTAVGGATGAALLLSTPAEVFEL
                     LVPLLIGGASLVLLLQPGINRLAGGMVDERGLALLAGVFAVGVYAGYFGAAAGVVLLA
                     LLTVSVAEPLARLVAARNVALGLANAVAAIGFALFGPVRWAAAAPLAVGFLIGGGLGP
                     GLVRRLPGGRLRVAIALAGLALAVKLGIDTFGQAGTAG"
     gene            1424..2473
                     /locus_tag="VFO47_02880"
     CDS             1424..2473
                     /locus_tag="VFO47_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET8787677.1"
                     /translation="MTECVLSTRELNRALLARQLLLERSSLSLPDALEQVAGLQAQYA
                     PSPYVGLWSRLRDFRRDDLTRALEDRRAVQATLLRVTIHVVSAADYPPFTEAVRGARR
                     EWWARAARRELEGVDVEAAAALVRERLQAGPARQAELARLLADRGYPKAVWTGVGLWV
                     DMVRVPPSGTWERRRADLYGLADDWLGPWRVDEADALEHLVRRYLAGFGPASLADLGS
                     WAGLSLTPLRPVLEQVGTRRFRDEQGTELFDLPGAPLPEEGTPAPPRFLSNWDANLLV
                     HARRAQVLPDRHRPRIFHVRAPHSFPTFLVDGAVAGTWRHQRGRVLLEPFEPLAAATR
                     RQLEDEAERLAAFHH"
BASE COUNT          249 a          976 c          920 g          349 t
ORIGIN      
        1 cgacatgtcg gcccgcatcg ccctgcaccg cggggccggc cgggtcatcg gggtcgacct
       61 ggtccccgag cggctggagc gggcccgggc ccggggcgtc gagacccttg acctgaacga
      121 caccgacgac ctggtcgaga ccgtccgcga cctcaccgcc ggccgcggcc ccgacgcggt
      181 gatcgacgcc gtcggcatgg aggcccacgg gtcgccggtc ggcaagctgg cccaccagat
      241 ggcctcgctg ctgcccgacg ccctggccgc caagatgatc gagacggccg gcgtggaccg
      301 cctcaacgcc ttccacctgg ccatcgagct ggtccgccgg ggcgggacga tctcgctgtc
      361 cggcgtctac ggcggcatgg ccgacccgct gcccatgctc accctgttcg acaagcagct
      421 ccagctccgc atgggccagg ccaacgtccg ccgctgggtc ggcgacatcc tccccctgct
      481 caccgacgac gacccgctcg gggtcgacac cttcgccacc caccacctcc ccctggccga
      541 cgcccccagg gggtacgaga tcttccagaa gaagcaggac ggcgccgtca agatcctgct
      601 gcagccgtga ccagcccacc cgggccgtag aatcccgggc ggtgcacggc gcggacgtgg
      661 tcttcctcct ggtcgccggg gtcctcgccg gggtcgccgg ctcggtcgcc ggcctggcgt
      721 cgttggtgtc gtacccggcc ctgctggcgg tggggctgcc ggcgctgacc gcgaacgtga
      781 ccaacacggt ggcgctggtg gggtacacgg tctcggcggc ggcgttctcg cgccaggagc
      841 tggccggcca ggcggggcgg gtgcgccgcc tggccggcgt cacggccgtc ggcggcgcca
      901 ccggggcggc gctgctgctg tcgacccccg ccgaggtgtt cgagctgctg gtgccgctgc
      961 tgatcggcgg cgcctccctg gtcctgctgc tccagccggg catcaaccgg ctcgccgggg
     1021 gcatggtcga cgagcgcggc ctggccctgc tggccggcgt gttcgccgtc ggggtgtacg
     1081 ccggctactt cggggcggcg gccggggtgg tgctgctggc cctgctgacc gtgtcggtgg
     1141 ccgagccgct ggcccggctg gtggccgccc gcaacgtcgc cctggggctg gccaacgccg
     1201 tggccgcgat cggcttcgcc ctgttcggcc ccgtccgctg ggcggcggcg gcgccgctgg
     1261 cggtcgggtt cctgatcggc ggcggcctcg gtcccggcct ggtccgccgg ctgccgggcg
     1321 ggcgcctgcg ggtcgcgatc gccctggccg ggctggccct ggccgtcaag ctggggatcg
     1381 acaccttcgg ccaggccggg accgccgggt agactgcggc cccgtgaccg agtgcgtgct
     1441 gtccacccgc gagctcaacc gggccctgct tgcccgccag ctgctcctcg agcgctcctc
     1501 gctgtccctt cccgacgccc tcgagcaggt cgccggtctc caggcccagt acgccccgtc
     1561 gccgtacgtc ggcctgtggt cgcggctgcg cgacttccgc cgcgacgacc tcacccgggc
     1621 gctggaggac cggcgggccg tccaggcgac cctgctccgg gtcaccatcc acgtcgtctc
     1681 ggccgccgac tacccgccgt tcaccgaggc ggtccggggc gcccgccgcg agtggtgggc
     1741 ccgagcggcc cgccgcgagc tggagggcgt cgacgtcgag gcggccgccg ccctggtccg
     1801 cgagcgcctc caggccggcc cggcccgcca ggccgagctg gcccggctgc tcgccgaccg
     1861 gggatacccc aaggcggtgt ggaccggggt cgggctctgg gtcgacatgg tccgggtccc
     1921 gccgtctggc acctgggagc ggcgccgggc cgacctgtac ggcctggccg acgactggct
     1981 cgggccgtgg cgggtcgacg aggccgatgc gctcgagcac ctggtccggc gctacctggc
     2041 cgggttcggc ccggcctccc tggccgacct gggcagctgg gccgggctgt ccctgacccc
     2101 gctgcggccg gtgctggagc aggtcggcac ccgccggttc cgcgacgagc agggcaccga
     2161 gctgttcgac ctgcccggcg cgcccctgcc cgaggagggc acgccggccc cgccccgctt
     2221 tctgtccaac tgggacgcca atctgctggt gcacgcccgg cgcgcccagg tcctgcccga
     2281 ccggcaccgg ccccgcatct tccatgtcag ggccccgcac tcgttcccga ccttcctggt
     2341 cgacggggcg gtggccggga cctggcgcca ccagcggggc cgggtgctgc tggagccgtt
     2401 cgagccgctg gccgccgcga cccgccgcca gctggaggac gaggccgaac gcctggccgc
     2461 cttccatcac tgacggctcg ccgatttggt ccag
//
LOCUS       DASSWR010000175         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_910774,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000175 DASSWR010000000
VERSION     DASSWR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910774"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>2134
                     /locus_tag="VFO47_02885"
     CDS             53..>2134
                     /locus_tag="VFO47_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787678.1"
                     /translation="MPRCAGCGQDNPGGFRFCGACGASLAGPVQAPAEERRLVTVLFC
                     DLVGFTARSDQADPEDVGALLRPYHIRLRAEIERLGGTLDKFVGDGVMAVFGAPVAHE
                     DDPERAVRCALAMLAASEELNLTVRIGITTGEALVRFGPARQTEGVVGDVVNTASRLQ
                     GVAPAGGVVVGEASFRATRRLFDYQELGPVQVKGKADPVPVWQLQGARSRTGIEAVRR
                     AGTPFVGRQAELERLRGLFEQTLADRTVRLVTVVGEPGVGKSRFVSELAADVDARPEL
                     VTWRQGRCLPYGDGITFWALGEIVKAQAGVLESDPPAEVSGKLRAVIADLLPDPSERE
                     WLRARLAPLLGIADADAVKAERAELFAAWQRFVEAMAASHPLVLVVEDLHWADAAMLE
                     FLEHLVERSSDLPLLIVATARPELLERRPGWGGGTPAATRIPLAPLSDLEVARLVAAL
                     VGRTSLPVGVQALLLERAGGNPLYAEEFARLLADQGMVAGEDAAVPDIPVPETVHGLI
                     AARLDALAPEVRALVQNAAVVGRVFWPGAVAAMDGDGGSGGLEASPPVDGGRAGDQTV
                     HAGLAELERKQLVQRARTSSVQHQDEYVFWHALVRDVAYAQIPRAGRARRHQAVAEWV
                     EAVAGERVGDLAEVVAHHYGQALAYARAAREPQEQIDKLIEPTRRFLVLAGDRTINLD
                     LDRARTYYRQAV"
BASE COUNT          253 a          793 c          811 g          277 t
ORIGIN      
        1 gtttccacac cccggtcaac cacggtcggt ggccgccccg tagactgccc ccatgccgcg
       61 ctgcgcaggt tgcggacagg acaacccggg gggcttccgg ttctgcgggg cctgcggggc
      121 gtccctcgcc ggaccagtcc aggccccggc cgaggagcgc cgcctggtca cggtgctgtt
      181 ctgcgacctg gtgggcttca ccgcccgctc cgaccaggcc gaccccgagg acgtcggcgc
      241 cctgctgcgc ccctaccaca tccggctgcg ggccgagatc gaacgcctcg gcggcaccct
      301 ggacaagttc gtcggcgacg gcgtcatggc cgtcttcggc gcccccgtcg cccacgagga
      361 cgaccccgaa cgagccgtcc gctgcgccct ggccatgctg gcggccagcg aggagctcaa
      421 cctgaccgtc cgcatcggca tcaccaccgg cgaggccctg gtccgcttcg gcccggcccg
      481 ccagaccgag ggcgtggtcg gggacgtggt caacacggcc tcgcggctgc agggggtggc
      541 cccggcgggt ggggtggtgg tgggggaggc gagcttccgg gcgacccggc ggctgttcga
      601 ctaccaggag ctggggccgg tccaggtcaa gggcaaggcc gacccggtgc cggtgtggca
      661 gctgcagggg gcccgcagcc gcaccggcat cgaggccgtg cgccgggccg gcaccccctt
      721 cgtcggccgc caggccgagc tggagcgcct caggggcctg ttcgagcaga ccctggccga
      781 ccggaccgtg cgcctggtca cggtcgtggg cgagccgggg gtgggcaagt cccgcttcgt
      841 gagtgagctg gccgccgacg tggacgcccg gcccgagctg gtcacctggc gccagggccg
      901 ctgcctgccc tatggcgacg ggatcacctt ctgggcgctg ggcgagatcg tcaaggccca
      961 ggccggggtc ctcgagtccg accccccggc cgaggtgtcg ggcaagctcc gggcggtcat
     1021 cgccgacctg ctccccgacc cgtcggagcg cgagtggctg cgggcgcggc tcgcccccct
     1081 gctcgggatc gccgacgccg acgcggtcaa ggccgagcgg gccgagctgt tcgccgcctg
     1141 gcagcgcttc gtcgaggcca tggccgcctc acacccgctg gtgctggtgg tggaggacct
     1201 ccactgggcc gacgcggcca tgctcgaatt cctcgagcat ctggtcgagc ggtccagcga
     1261 ccttcccctg ctgatcgtgg ccacggcccg tcccgagctg ctggagcggc gcccgggctg
     1321 gggcggcggc actccggccg cgaccaggat cccgctggcg ccgctgagcg acctggaggt
     1381 ggcgcggctg gtggccgccc tggtgggcag gacgtcgctg cccgtcgggg tgcaggcctt
     1441 gctgctggag cgggccggtg gcaacccgct gtacgccgag gagttcgccc ggctgctggc
     1501 cgaccagggc atggtcgccg gcgaggacgc ggccgtcccc gacatccccg tccccgagac
     1561 cgtgcacggg ctgatcgcgg cccgcctcga cgccctcgcc cccgaggtca gggcgctggt
     1621 ccagaacgcc gccgtggtcg gccgggtctt ctggccgggc gccgtggccg ccatggacgg
     1681 cgacgggggc tccggggggc ttgaggccag ccccccggtg gacggcgggc gcgccggtga
     1741 ccagacggtc catgccggcc tggccgagct ggagcgcaag cagctggtcc agcgggcccg
     1801 gacctcgtcg gtccagcacc aggacgagta cgtgttctgg cacgccctgg tcagggacgt
     1861 ggcctacgcc cagattccca gggcgggccg ggcccgccgc caccaggcgg tcgccgagtg
     1921 ggtcgaggcc gtggccgggg agcgggtcgg ggacctggcc gaggtcgtcg cccaccacta
     1981 cggacaggcg ctggcctacg ccagggcggc ccgggagccc caggagcaga tcgacaagct
     2041 gatcgagccg acccgccgct ttctcgtcct ggccggggac cggacgatca acctcgacct
     2101 cgaccgggcc cgcacctact accggcaggc cgtc
//
LOCUS       DASSWR010000176         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1052696, whole genome shotgun sequence.
ACCESSION   DASSWR010000176 DASSWR010000000
VERSION     DASSWR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052696"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1211)
                     /locus_tag="VFO47_02890"
     CDS             complement(33..1211)
                     /locus_tag="VFO47_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8787679.1"
                     /translation="MTDSSIGVAVIGAGMAGRAHAAGYRSASTVYGEGLPEIRLAAIA
                     DVNPVFAADTARRFGFERAEGGWEAVAADDDVDVVSVVVANPLHRPIVEGLLDAGKHV
                     LCEKPMAPTVEDAEAMVAAAKASGRETGIGFVFRRSPAIGAIREQLASGAMGRPLHFN
                     GHYWCDYGVDPRGPMSWRYKGGPGSGALSDIGSHLIDLSEFVCGPIRSVRGAVLSTFV
                     TERALPAGAAIGHAAAELSDVTEPVENEDLVTFTATFESGATGTLSASRSAYGLANSL
                     GFELFTSSGAATFDLARAGEFGFIDGAPAGPTQGFRQVLVGPAHPYLTGGLPMDFPGV
                     GYGQNDLFTFQARAFLEQVAGVEGLPRLASFEEGLHNIRLLDAIVASAKAGGADVTVN
                     "
     gene            1291..>2171
                     /locus_tag="VFO47_02895"
     CDS             1291..>2171
                     /locus_tag="VFO47_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010353496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8787680.1"
                     /translation="MSRGSRRPRLEDVAERVGMSPASVSMVLSNAPGPSPATRERVLQ
                     AAAELGYRPDRTASLLARRRTHLVGVLMQVSSPFHAELVEDLHEAAEGRGYDVVLSTV
                     TRTRDERRATETLVDFRCEALVLLGPVAPAAQLAALGRQLPVVVIGRRVAGGGVDVVR
                     TADAEGVGQVVGHLAGLGHRSIAYVDGGRGTIAADRRRGYRTAMRRHGLAGQVRVIGG
                     DHTEAAGSRAARTLLDDGALPGAVVAYNDSCALGLLDAFNRAGVEVPGRVSVAGYDDS
                     TLARLAHVDLTTVSQDAR"
BASE COUNT          284 a          784 c          832 g          271 t
ORIGIN      
        1 gtaggcgccg agcttcatgt tcgtcctccc ggtcagttga cggtcacgtc ggcgccgccg
       61 gccttggccg aggcgacgat cgcgtccagc aggcggatgt tgtgcagccc ctcctcgaag
      121 gaggccagcc gggggagccc ctccaccccc gcgacctgct cgaggaaggc ccgggcctgg
      181 aaggtgaaca ggtcgttctg cccgtagccg accccgggga agtccatcgg gaggcccccg
      241 gtgaggtacg ggtgggcggg cccgaccagc acctggcgga acccctgggt cgggccggcc
      301 ggggcgccgt cgatgaagcc gaactcgccg gcccgggcca ggtcgaaggt ggccgcgccc
      361 gaggaggtga acagctcgaa accgagggag ttggccaggc cgtaggcgga ccgggaggcc
      421 gacagggtgc cggtggcgcc ggattcgaag gtggccgtga aggtgaccag gtcctcgttc
      481 tccaccggct cggtgacgtc gctgagctcg gcggcggcgt ggccgatggc cgcgcccgcg
      541 gggagggcac gctcggtgac gaaggtggac agcaccgcgc cccggaccga ccggatgggg
      601 ccgcagacga actcggacag gtcgatcagg tggctgccga tgtcggacag ggcgcccgac
      661 cccgggccgc ccttgtagcg ccagctcatc gggccccgtg ggtcgacccc gtagtcgcac
      721 cagtagtggc cgttgaagtg caggggccgg cccatggcgc cggaggcgag ctgctcccgg
      781 atggcgccga tggcggggga gcggcggaac acgaacccga tgccggtctc gcggcccgac
      841 gccttggccg cggccaccat ggcctcggcg tcctcgaccg tcggggccat cggcttctcg
      901 cagagcacgt gcttgcccgc gtccagcagg ccctcgacga tcgggcggtg gagcgggttg
      961 gccacgacca cgctgaccac gtcgacgtcg tcgtcggcgg ccacggcctc ccagccgccc
     1021 tcggcccgct cgaacccgaa gcggcgggcg gtgtcggcgg cgaagacggg gttgacgtcg
     1081 gcgatcgcgg ccagccggat ctccggcagg ccctcgccgt agacggttga cgccgaccgg
     1141 tacccggcgg cgtgggcacg gccggccatg ccggccccga tgacggcgac gccgatggac
     1201 gagtcggtca tgaggtgcgc ctccttcctg agggtttgaa gcgcttgatt tgaagcgctt
     1261 caaaccgtac tatgcgaggc atccatggac atgtcaagag gttcgcgcag accacggctc
     1321 gaggacgtcg ccgagcgggt cgggatgtcg ccggcctcgg tgtcgatggt gctgtccaac
     1381 gcccccggcc ccagcccggc cacccgcgag cgcgtcctgc aggcggccgc cgagctcggc
     1441 taccgccccg accggacggc cagcctgctc gcccggcggc ggacccacct ggtcggggtg
     1501 ctgatgcagg tgagcagccc gttccacgcc gagctggtcg aggacctcca cgaggccgcc
     1561 gagggccgcg gctacgacgt cgtcctgagc acggtgaccc gcacccgcga cgagcgccgg
     1621 gccaccgaga cgctggtcga cttccgctgc gaggccctcg tcctcctcgg cccggtcgcc
     1681 cccgccgccc agctcgccgc cctggggcgg cagctgccgg tggtggtgat cgggcgccgg
     1741 gtcgccgggg gcggggtcga cgtggtccgg acggccgacg ccgagggggt cggccaggtc
     1801 gtcggccacc tggccgggct ggggcaccgt tcgatcgcgt acgtcgacgg gggccggggg
     1861 acgatcgccg ccgaccgccg ccgcggctac cgcacggcca tgcgccgcca cggcctggcc
     1921 ggccaggtgc gggtcatcgg cggcgaccac accgaggcgg ccggcagccg ggcggcgcgg
     1981 acgctgctgg acgacggcgc cctccccggc gcggtggtcg cctacaacga ctcctgcgcg
     2041 ctcgggctgc tggacgcctt caaccgggcc ggggtcgagg tccccgggcg ggtctcggtg
     2101 gccggctacg acgacagcac cctggcccgg ctcgcccacg tcgacctgac cacggtcagc
     2161 caggacgccc g
//
LOCUS       DASSWR010000177         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_657387,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000177 DASSWR010000000
VERSION     DASSWR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657387"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1727)
                     /locus_tag="VFO47_02900"
     CDS             complement(144..1727)
                     /locus_tag="VFO47_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787681.1"
                     /translation="MATEATEATEGGPEEARPRSPAIECRGITRRYAGVVANDQIDLQ
                     VTEGEIHALVGENGAGKSTLMKMLYGMEQPDEGEILVRGEPQVIDSPRRAIELRIGMV
                     HQHFMLVDDFTVAENIVLGAEPTRGGGRIDRDAAEQRVGELARGFGTPLDPHARVEDI
                     GVGQQQRVEILKLLYRGADILILDEPTAVLVPQEVEELFDHMRQLKADGKTIIFIAHS
                     LDEVLQIADRITVLRDGKVIDTVVAADTDTHQVAEMMVGRPVLLRRVEGKATPGGPML
                     QVKDLRVSVGGKDLVAGLDLDVRRSEIYGLAGVEGNGQAELVEALVGLTAPDTGRIYL
                     DDDELTRADVRTRRQAGLAYIPEDRHARGMVLQMLVSENSVLGQQEREPFSRRGLLDL
                     RAIRRRAVELIRGFRVKAPSVSAPAYVLSGGNQQKLVLGREFEINPKLLIAAHPTRGL
                     DIGATQFVWQELVEARDEGVAVLLISSNLEEILALADRVGVIHDGRIGATFRGSEATR
                     TELGLYMTGARGGDEEPAA"
     gene            complement(1730..2806)
                     /locus_tag="VFO47_02905"
     CDS             complement(1730..2806)
                     /locus_tag="VFO47_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8787682.1"
                     /translation="MRGVRGVRVGALLVAFGLMVAACGGGDDEGGDAGTGGGAETKQV
                     GLVYDIGGRGDQSFNDSAGAGLDAAKQEQGDKIEVREIEPNADGSNRKELLDSLAEEG
                     YGLVFGVGFLFSEDIAKSATEAPDTTFAVIDGFDDLCTEQGKNLLCIAFKEEEGSFLV
                     GAAAALKTESNTVGFVGGLEGDLIKKFQAGYEAGVKYIADQEGKDIEVLVDYAGNTPE
                     AFRNPAKGKELALKQIGDGADVIYHASGGTGAGVISAAAAEQVYAIGVDSDQSLTASE
                     AERKWILTSMLKRVDTAVQETIATYVGGQVPGGFRVFGLKEGGIDYAQNEYNKELLGD
                     IPTTLDDLKQKIADGEIKVPDKPS"
     gene            2926..>3233
                     /locus_tag="VFO47_02910"
     CDS             2926..>3233
                     /locus_tag="VFO47_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET8787683.1"
                     /translation="MRRVLLGLLVLVLAYFAVTTTLVARWMGKDERPRVDAIVVLGAA
                     QYDGRPSAIYEARLEHAVQLWRDGVAPLLVFTGGKEEGDRFTEGGSGARWAIQRGVPS
                     R"
BASE COUNT          394 a         1163 c         1155 g          521 t
ORIGIN      
        1 gacgccgatg acggccgcaa tcagcagcga cacggccggg gcgagcagct ggaaggccca
       61 gccccgcagg cgctggcccc aggggacccg ggcggcggcc tcctcgacct ccggctcggg
      121 cggcggtggc cgctcttcga ccttcacgcc gccggctcct cgtccccgcc gcgggcgccg
      181 gtcatgtaca ggccgagctc ggtcctggtc gcctccgagc cgcggaaggt ggccccgatg
      241 cggccgtcgt ggatgacccc gacccggtcg gccagggcca ggatctcctc caggttggag
      301 gagatcagca gcacggccac gccctcgtcg cgggcctcga ccagctcctg ccagacgaac
      361 tgggtggccc cgatgtccag gccgcgggtc gggtgggcgg cgatcagcag cttggggttg
      421 atctcgaact cgcggccgag gaccagcttc tgctggttgc cgccggacag gacgtaggcc
      481 ggggcggaca cgctgggcgc cttgacccgg aagccgcgga tcagctcgac cgcccggcgc
      541 cggatggccc ggaggtcgag caggccgcgc cggctgaacg gctcgcgctc ctgctggccg
      601 aggaccgagt tctcgctgac cagcatctgc aggaccatgc cgcgggcgtg gcggtcctcg
      661 gggatgtagg ccaggccggc ctggcgccgg gtgcgcacgt cggcccgggt cagctcgtcg
      721 tcgtccaggt agatccggcc ggtgtcgggg gcggtcaggc cgacgagggc ctcgaccagc
      781 tcggcctggc cgttgccctc gaccccggcc agcccgtaga tctccgagcg gcgcacgtcc
      841 aggtcgagcc cggccaccag gtccttgccc ccgacgctca cccggaggtc cttgacctgg
      901 agcatcggcc cgccgggggt ggccttgccc tcgacccggc gcagcagcac cgggcggccg
      961 accatcatct cggccacctg gtgggtgtcg gtgtcggcgg cgacgacggt gtcgatcacc
     1021 ttgccgtcgc ggagcacggt gatccggtcg gcgatctgaa gcacctcgtc cagcgagtgg
     1081 gcgatgaaga tgatcgtctt gccgtcggcc ttgagctggc gcatgtggtc gaacagctcc
     1141 tcgacctcct gggggacgag cacggccgtc ggctcgtcca ggatcaggat gtccgcgccc
     1201 cggtagagga gcttgaggat ctcgacccgc tgctgctggc cgacgccgat gtcctcgacc
     1261 cgggcgtgcg ggtccagcgg ggtgccgaac ccgcgggcca gctcccccac ccgctgctcg
     1321 gccgcgtccc ggtcgatccg gccgccgccg cgggtcggct cggccccgag cacgatgttc
     1381 tcggccacag tgaagtcgtc gaccagcatg aagtgctggt ggaccatgcc gatgcgcagc
     1441 tcgatggccc gccggggcga gtcgatcacc tgcggctcgc cccggacgag gatctcaccc
     1501 tcgtcgggct gctccatccc gtagagcatc ttcatcaggg tcgacttgcc ggcgccgttc
     1561 tcgccgacca gggcgtggat ctcgccctcg gtcacctgca ggtcgatctg gtcgttggcg
     1621 accaccccgg cgtagcggcg ggtgatgccg cggcactcga tggccggcga ccggggacgg
     1681 gcctcctcgg gcccgccctc ggtcgcctcg gtcgcctcgg tcgccacggc taggacggct
     1741 tgtccgggac cttgatctcg ccgtcggcga tcttctgctt caggtcgtca agggtggtcg
     1801 ggatgtcgcc caggagctcc ttgttgtact cgttctgggc gtagtcgatc ccgccctcct
     1861 tgagcccgaa gacccggaag ccgccgggga cctggccgcc gacgtaggtg gcgatggtct
     1921 cctggacggc ggtgtcgacc cgcttgagca tggaggtgag gatccacttc cgctcggcct
     1981 cgctggccgt caggctctgg tcggagtcga ccccgatggc gtagacctgc tcggcggcgg
     2041 cggccgagat gaccccggcc ccggtgccgc cggaggcgtg gtagatgacg tcggcgccgt
     2101 cgccgatctg cttcagggcc agctccttgc ccttggccgg gttgcggaag gcctcggggg
     2161 tgttgccggc gtagtcgacc aggacctcga tgtccttgcc ctcctggtcg gcgatgtact
     2221 tgacgcccgc ctcgtagccg gcctggaact tcttgatgag gtcgccctcg agcccgccga
     2281 cgaagccgac ggtgttggac tcggtcttga gggcggccgc ggccccgacc aggaacgagc
     2341 cttcctcctc cttgaaggcg atgcacagca ggttcttgcc ctgctcggtg cagaggtcgt
     2401 cgaagccgtc gatcaccgcg aaggtggtgt cgggggcctc ggtggccgac ttggcgatgt
     2461 cctcggagaa caggaacccg accccgaaga ccaggccgta gccctcctcg gcgaggctgt
     2521 cgagcagctc cttgcggttg gagccgtcgg cgttgggctc gatctccctg acctcgatct
     2581 tgtcgccctg ctcctgcttg gccgcgtcga gcccggcgcc ggccgagtcg ttgaaggact
     2641 ggtcgccgcg gccgccgatg tcgtagacca ggccgacctg cttggtctcg gcgccgccgc
     2701 cggtgccggc gtcgccgccc tcgtcgtcgc cgccgccgca cgcggccacc atcaggccga
     2761 acgccaccag cagggcgccc acccgcacac cccgtacccc acgcatgcac tgctcctctc
     2821 ggacgttggg ctcccaccct cgcccgcgcc gctggctggc ggtatttaac cactccttgg
     2881 gctggtggcc agggtgccgt cgggcggctg cgtagaatcc ccggcatgcg ccgcgtcctg
     2941 ctgggcctcc tcgtgctcgt gctcgcctac ttcgccgtga ccactacgct ggtcgcccgg
     3001 tggatgggca aggacgagcg tcccagggtc gacgccatcg tggtcctggg tgcggcccag
     3061 tacgacgggc ggccctcggc catctacgag gcccgccttg agcacgccgt ccagctgtgg
     3121 cgggacgggg tggccccgct gctggtgttc accgggggca aggaggaggg cgaccgcttc
     3181 accgagggcg ggtcgggggc ccgctgggcc atccagcgcg gcgtgccctc ccg
//
LOCUS       DASSWR010000178         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_233471,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000178 DASSWR010000000
VERSION     DASSWR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233471"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFO47_02915"
     CDS             complement(<1..982)
                     /locus_tag="VFO47_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8787684.1"
                     /translation="MSASEDASKHLRVAVVAPPWFTIPPDGYGGIEAMVHWLVEGLVA
                     RGHQVTLIGAGDCRTSASFLQTYEEPPAARLGTPFPEIVHALQVDEHLEALDVDVVHD
                     HSMGGPLTARARRASTVLTAHGPVVGELGQCYRLLSRHHPLVAISESQRAKGPHLPWA
                     ATVYNAIPVDEYPFETDKDDFVLFLGRMSPEKAPDLAIKAARAAGRRIVVAAKCNEPA
                     EHAYFEERVRPLLGHDAEWYGHATTEEKKKLLARAGALIFPIQWDEPFGIVMVEAMAC
                     GTPVVALRAGSVPELVVDGLTGYICDRPEELPAAIRRVDALDPRDCRQRVS"
     gene            1099..2547
                     /gene="gltX"
                     /locus_tag="VFO47_02920"
     CDS             1099..2547
                     /gene="gltX"
                     /locus_tag="VFO47_02920"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET8787685.1"
                     /translation="MSERVRTRFAPSPTGFLHVGTARTALYSYLFARRHGGQFVLRIE
                     DTDTGRNVADSAAGIVDGLRWLGLDWDEGYQRGGPHGPYVQTERLDRYHAAVNALVAH
                     GDAYLCYCTNEELEQRRQAAAAHGLPPGYDGHCRHLRPDQVRAYEAEGRRPAVRFRLP
                     DEGETVVEDLVRGTVPFDNATLTDFVILRANGVPTYHFSAVYDDVDMGITHVIRGEDL
                     FPSTPLHVHVFRALGRETPPAFAHLPLIVGADRRKLSKRRDKMSVQQFRDDGYLAEAM
                     VNYLALLGWSLDDHTELFALADLERVFSLERVSRNPAAFDVKKLDALNGHYLRQLDPA
                     AFAARAWPFVEAEALPEADPALLERASPLVQERVNHLTEVPGMVGFLMTDEVELDPAA
                     AAKALTEDGRAFLAEAAARLDKLEPWTAAAIEETLRGLQTERGLKPKVAFQPVRVAVT
                     GRLVSPPLFESMELLGKPRSLARLDRAAALAG"
     gene            2607..2678
                     /locus_tag="VFO47_02925"
     tRNA            2607..2678
                     /locus_tag="VFO47_02925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2640..2642,aa:Gln,seq:ctg)
     gene            2694..2769
                     /locus_tag="VFO47_02930"
     tRNA            2694..2769
                     /locus_tag="VFO47_02930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2728..2730,aa:Glu,seq:ctc)
BASE COUNT          395 a         1061 c          997 g          400 t
ORIGIN      
        1 cggacacccg ctggcggcag tccctggggt cgagggcgtc gacccgccgg atggccgcgg
       61 gcagctcctc ggggcgatcg cagatgtagc cggtgaggcc gtcgaccacc agctcgggca
      121 ccgagccggc gcgcagcgcc accaccgggg tgccgcaggc catggcctcg accatcacga
      181 tcccgaacgg ctcgtcccac tggatgggga agatcagcgc gccggccctg gccagcagct
      241 tcttcttctc ctcggtggtg gcgtggccgt accactcggc gtcgtggccg agcaggggac
      301 ggacccgctc ctcgaaatag gcgtgctcgg ccggctcgtt gcacttggcc gcgaccacga
      361 tccgccggcc ggcggcccgg gccgccttga tggccaggtc gggtgccttc tcggggctca
      421 tccgcccgag gaacagcacg aagtcgtcct tgtcggtctc gaacgggtac tcgtcgaccg
      481 ggatggcgtt gtagaccgtg gccgcccagg gcaggtgcgg gcccttggcc cgctgcgact
      541 cggagatggc gaccagcggg tggtggcggc tgagcagccg gtagcactgg cccagctcgc
      601 ccaccacggg cccgtgggcg gtgagcacgg tcgaggcccg gcgggccctg gccgtcagcg
      661 ggccgcccat cgagtggtcg tggaccacgt ccacgtccag cgcctccaga tgctcgtcca
      721 cctggagggc gtggacgatc tcggggaacg gggtgcccag gcgggcggcc ggcggctcct
      781 cgtaggtctg caggaagctg gcgctggtgc ggcagtcgcc ggcgccgatc agggtgacct
      841 ggtggccgcg ggcgaccagc ccctcgacca gccagtggac catggcctcg atgccgccgt
      901 agccgtcggg cgggatcgtg aaccagggag gcgcgaccac agcgacgcgc aggtgttttg
      961 aagcatcctc ggatgcggac acactggcct cctcaccgtt gggggtacgg cggtgccaag
     1021 ggcggagcgg tggagctggg gggctcctac cttaccgcga agcgccacct ccaaacgaac
     1081 tcgcgtagca ttcgtcgcat gagcgagcgc gttcggaccc ggtttgcacc ttcgccgacc
     1141 ggcttcctgc atgtcggcac tgcgcggacc gcgctgtact cgtacctttt cgcccgccgc
     1201 cacggcggcc agttcgtgct gcgcatcgag gacaccgaca ccgggcgcaa cgtggccgac
     1261 agcgccgccg ggatcgtgga cgggttgcgc tggctcggcc tcgactggga cgagggctac
     1321 cagcggggcg gcccccacgg gccgtacgtg cagaccgagc gcctcgaccg ctaccacgcg
     1381 gccgtcaacg ccctggtcgc ccacggcgac gcctacctct gctactgcac caacgaggag
     1441 ctggagcagc gccgccaggc ggccgcggcc cacggcctgc cgcccggcta cgacggccac
     1501 tgccgccacc tccggcccga ccaggttcgg gcctacgagg ccgaggggcg caggccggcg
     1561 gtccgcttcc ggctccccga cgagggcgag acggtggtcg aggacctggt ccggggcacg
     1621 gtccccttcg acaacgccac cctcaccgac ttcgtcatcc tgcgggccaa cggcgtcccc
     1681 acctaccact tctcggccgt ctacgacgac gtcgacatgg gcatcaccca cgtcatccgg
     1741 ggcgaggacc tgttcccgtc cacgcccctg cacgtgcacg tgttccgggc cctgggccgg
     1801 gagaccccgc ccgccttcgc ccacctgccc ctgatcgtcg gggccgaccg gcgcaagctg
     1861 tccaagcgcc gcgacaagat gtcggtgcag cagttccgcg acgacggcta cctggccgag
     1921 gcgatggtca actacctggc cctgctcggc tggtcgctgg acgaccacac cgagctgttc
     1981 gccctggccg acctggagcg ggtgttctcg ctggagcggg tctcgcgcaa cccggccgcc
     2041 ttcgacgtca agaagctcga cgccctcaac ggccactacc tgcgccagct cgacccggcc
     2101 gccttcgccg cccgggcctg gccgttcgtg gaggccgagg ccttgcccga ggccgacccg
     2161 gccctgctgg aacgggcctc gccgctggtc caggagcggg tcaaccacct gaccgaggtc
     2221 cccggcatgg tcgggttcct gatgaccgac gaggtcgagc tcgacccggc ggccgcggcc
     2281 aaggccctga ccgaggacgg ccgcgccttc ctggccgagg ccgcggcccg gctggacaag
     2341 ctcgagccgt ggacggccgc cgccatcgag gagaccctcc ggggcctcca gaccgagcgc
     2401 ggcctcaagc ccaaggtcgc cttccagccg gtccgcgtgg ccgtcaccgg ccgcctggtc
     2461 agcccgcccc tgttcgagtc catggagctg ctcggcaagc cccgctccct ggcccgcctg
     2521 gaccgggccg ccgccctggc cggttgaggg atccggccac cggccgctaa gatgcccgag
     2581 cccgtgggcc accggcccac gatccctggg gtgtggggta attggcagcc cgacgggttc
     2641 tggccccgtt agtctaggtt cgagtcctgg caccccagcg cagagcgcaa caaggccccg
     2701 tcgtctagcg gcctaggacg ccgccctctc aaggcggtag cgccggttcg aatccggtcg
     2761 gggctaccaa ccaccaccag cacaacacgg cctctgacct ggaggaatac gggtcggagg
     2821 ccgtgttgtg tgtccgacgg cgcccggtca ggt
//
LOCUS       DASSWR010000179         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1052762, whole genome shotgun sequence.
ACCESSION   DASSWR010000179 DASSWR010000000
VERSION     DASSWR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052762"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFO47_02935"
     CDS             <1..636
                     /locus_tag="VFO47_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET8787686.1"
                     /translation="GERLVDTLAEIHSVDPDSVGLGELGRKTDYVARQLHRWQGQWEM
                     GRTRDLPLVDEVHERLVSRIPEQGPATLVHGDYRLDNMILTPAGEVAAVVDWELCTLG
                     DPLADVGLLLVYWAEAGDQTLPLGSAPTVMPGFPGRAAVAEAYATGSGRDLSQLDTYV
                     AFASWKLAVILEGVVARHATGAYGGGDDTWRGFAEVVVQLADRALELTARL"
     gene            complement(704..2476)
                     /locus_tag="VFO47_02940"
     CDS             complement(704..2476)
                     /locus_tag="VFO47_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HET8787687.1"
                     /translation="MSLRPAVRREGPLDQVLLMIIVVLLGSTLMLWLTGQVAGFINSG
                     IWPQVSVPEMGRVMTRVLRHPLDPAAAWPADARKLMPGPVLFWATFLILLAVLAAAWV
                     YLSSALRSARRRMGRAIVVERTTAPADQVSPAGWARPQLFRELYVRAPTTWRVILGRV
                     NGRLVAAEPLQSVIALGPTQSQKTSGLAIPAILEWDGPVLAACVKPDLIRSTIGRRWQ
                     KGEVFLYDPAGATGMEANTWSPLPRCETWEGAYQMALSLVNASRLGSRADREGEPVPG
                     ATTNLLATMLLAAAISDRSMSDVLRWGQRQDRGEITAAIHVANEPAALDAFESILGLG
                     EQRRSQVYAQVLGVIAAYSDPTVKEASAGTRFTAERLLDGVANTAYVSLPAHEQRRLG
                     PLTVALIQDVIDLAFERSRNRDAPIDPALLVVLDDAASSAALPQLDMYAANAGRHGVQ
                     LVTTFRELSQMRARYDERAEAVFANHRAKVILSGVTDAETLALLSHLLGDETIRHLAT
                     PAALAKAAKDRGDGEPARTPRNRAGASPAETLGWISPGHGVLLYGHLPPAPITLRPWF
                     RDRQLQAMVDPAAAGAARRRTVRR"
BASE COUNT          333 a          925 c          975 g          334 t
ORIGIN      
        1 ggcgagcgcc tggtcgacac cctcgcagag atccattcgg tcgaccccga ctcggtcggc
       61 ctcggcgagc tcggccgcaa gaccgactac gtcgcgcgcc agctccaccg ctggcagggg
      121 cagtgggaga tggggaggac ccgtgacctg ccgctggtcg acgaggtgca cgagcgcctc
      181 gtgtcccgga tccccgagca gggcccggcc accctggtcc acggcgacta ccggctcgac
      241 aacatgatcc tgacgcccgc cggcgaggtg gcggccgtcg tggattggga gctgtgcacc
      301 ctcggcgacc cgctcgccga cgtcggcctg ctgctggtgt actgggccga ggccggcgac
      361 cagaccctcc ccctgggctc ggccccgacg gtcatgcccg gcttccccgg ccgcgccgcc
      421 gtggccgagg cctacgcgac cggctcgggt cgcgacctgt cgcagctcga cacctacgtg
      481 gccttcgcct cctggaagct cgccgtcatc ctcgaaggcg tggtcgcccg ccacgccacc
      541 ggagcctacg gtgggggcga cgacacctgg cgcggcttcg ccgaggtggt cgtccagctg
      601 gccgaccggg ccctggagct caccgcccgg ctgtgacccc gcgacggctc ggtgtcggcc
      661 ttcggccgac gatcaacgga cttcgaccgg cagagccggt cgactagcgc cggacggtcc
      721 ggcggcgggc ggcgccggcc gcggccgggt cgaccatcgc ctggagctgg cggtcgcgga
      781 accagggacg cagggtgatc ggggccgggg gcaggtggcc gtagaggagc accccgtgtc
      841 ccggggagat ccagcccagg gtctcggccg gggaggcccc ggcgcggttg cggggggtgc
      901 gggccggctc gccatcgcca cggtccttgg ccgccttggc cagggcggcc ggggtggcca
      961 ggtggcggat cgtctcgtcc cccagcaggt gcgacagcag ggccagggtc tcggcgtcgg
     1021 tgacgccgct caggatcacc ttggcccggt ggttggcgaa caccgcctcg gcccgctcgt
     1081 cgtagcgggc ccgcatctgc gacagctccc ggaaggtggt gaccagctgg accccgtggc
     1141 gcccggcgtt ggccgcgtac atgtccagct gcgggagggc ggccgagctg gcggcgtcgt
     1201 cgaggacgac cagcagggcc gggtcgatcg gcgcgtcccg gttgcgggag cgctcgaagg
     1261 ccaggtcgat gacgtcctgg atgagggcga cggtgagcgg gcccaggcgc cgctgctcgt
     1321 gcgcgggcag gctcacatag gcggtgttgg ccaccccgtc gagcaggcgc tcggcggtga
     1381 accgggtccc cgccgacgcc tccttgaccg tcgggtcgga gtaggcggcg atcacgccca
     1441 gcacctgggc gtagacctgg ctgcggcgct gctcgccaag gcccaagatc gactcgaagg
     1501 cgtcaagggc cgcgggctcg ttggccacgt ggatggcggc ggtgatctcg ccccggtcct
     1561 ggcgctggcc ccagcggagc acgtcgctca tggaccggtc ggagatggcc gcggccagca
     1621 gcatggtggc cagcaggttg gtggtggccc cggggaccgg ctccccctcc cggtcggccc
     1681 ggctgcccag gcggctggcg ttcaccaggg acagggccat ctggtaggcg ccctcccagg
     1741 tctcgcagcg gggcagaggc gaccaggtgt tggcctccat cccggtggcc ccggccgggt
     1801 cgtagaggaa cacctcgccc ttctgccagc gccggccgat ggtggagcgg atcaggtcgg
     1861 gcttgacgca ggcggccagc accggcccgt cccactccag gatggccggg atggccaggc
     1921 cgctggtctt ctggctctgg gtcgggccga gggcgatcac cgactgcagc ggctcggccg
     1981 cgaccaggcg cccgttgacc cggccaagga tgacccgcca ggtcgtcggc gcccgcacgt
     2041 acagctcccg gaacagctgc gggcgggccc agccggccgg gctgacctgg tcggccgggg
     2101 cggtggtgcg ctcgaccacg atggcccggc ccatgcgccg gcgggccgac cgcagcgccg
     2161 acgacaggta cacccaggcg gcggccagga cggccagcag gatcaggaag gtcgcccaga
     2221 acagcaccgg gccgggcatg agcttgcggg cgtcggccgg ccaggcggcg gccgggtcga
     2281 gcgggtgccg cagcaccctg gtcatcaccc gccccatctc cggcaccgac acctgcggcc
     2341 agatgccgga gttgatgaac ccggcgacct gcccggtcag ccacagcatc aaggtcgacc
     2401 cgagcaggac cacgatgatc atcagcagga cctggtcgag tggcccctcg cgccggaccg
     2461 ccggccgcag gctcatgacg ccgagtcgac ggcctcgtcg gcctcggagg tggcccacgg
     2521 tgccgaccgg gcgcccatgt tggcgtcggt gtcgacgatc tccttct
//
LOCUS       DASSWR010000180         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_6016,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000180 DASSWR010000000
VERSION     DASSWR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6016"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFO47_02945"
     CDS             complement(<1..321)
                     /locus_tag="VFO47_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HET8787688.1"
                     /translation="MLAVDLLHRGHLFLKRLYVLFVIEVATRQVQVLGVTAHPVGEWL
                     TQQARNLLMTLDDRAGRFRFLVRDRDTKFTAAFDAVFAAEAIKVLMTPVRAPRAKPMR
                     SGGWA"
     gene            complement(1163..2413)
                     /gene="glpB"
                     /locus_tag="VFO47_02950"
     CDS             complement(1163..2413)
                     /gene="glpB"
                     /locus_tag="VFO47_02950"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpB"
                     /protein_id="HET8787689.1"
                     /translation="MGADVVVIGAGPSGLAAAAMLAEAGRQVQVIARGHGFTHWAAGG
                     IDVLGRLPAGDEAGAGEPVERPLEALGRLPDGHPYSLVGEGALLAGIGHVRALTDRAG
                     IELVGDPAVNRSQITGIGTRRTTALLQTQSDGALAGRVAAIGFAGFEDFSSHLCADRL
                     HRIGLDAVPIEIPLPQGFRGRHVNAVELARAFDGREFRGAVAGTLGRAAAGVDVCVVP
                     AVIGLANAAEAWADLQELAGLRVVEAALPPPSIPGLRLFDAWRERLSELGVGWRLGLP
                     AIGAERDGDRVTAILGEGASGPVRIPCDEAVLATGGVAGGGIETYRDGTLAETVLGLP
                     IDGFAHRTEFLAADFFGSHRLAQAGVRVNRELRPVDRAGASVLGNVRVIGTQLAGHDP
                     TAEGSREGVGLATAARAAELLAGA"
     gene            complement(2413..4161)
                     /gene="glpA"
                     /locus_tag="VFO47_02955"
     CDS             complement(2413..4161)
                     /gene="glpA"
                     /locus_tag="VFO47_02955"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HET8787690.1"
                     /translation="MLRETDVVVIGGGATGTAVLWDLALRGIEAVLVDRSDLGTGTSG
                     RWHGELHSGARYAVNDLPSARECVEENAVLRRIAPHTIEDTGGFFILLPEHDLGYGDR
                     FVTGCAETGVPCEEVDPAAARREEPALTDRIERAFWVPTDGSMDAWRLLWSMAYGAEA
                     RGGRVLVHHPVVGFERDGDGRVSAVRVHDLVGGRGRTIGCRWVINAAGAWAGRIGAMA
                     GAPFRMVPDKGAMVVMAGRYVRSLVTHCRMPTDGDIIVPAYDAAILGTTSTEADDADD
                     PSVAPLEIDLIIDQTAKLVPAIANGRVLRAYSGYRPLYDPVQGEAGGHGRSVSRTFTV
                     LDHEPGDGVPNLLSIVGGKLTTSRQMAQKVVDVMVAKRGEERPCTTAITPLPGAEQGI
                     RQPTRPLARPSTAAGQADGPLACECELITRRTIEEAVGAGVTQLDDLRRQYRLGFGPT
                     QGGGCGWRAAAVVAARRPDVGDPLANLRLFMQERWRGLRAVAWGQAAVQALRNDAIYR
                     GILALDTVEREPGEDEQVQAAATGDDWSGPRQAGPGRGVGGEGLTGDTEGRRATDAGP
                     PGGPRGPLPEPTEGGR"
     gene            4264..4389
                     /locus_tag="VFO47_02960"
     CDS             4264..4389
                     /locus_tag="VFO47_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787691.1"
                     /translation="MPARRPVQAAVDRRFNRCRYNAARTIQAFSARLRQQIELDT"
BASE COUNT          680 a         1741 c         1574 g          612 t
ORIGIN      
        1 tgcccaccca ccgctccgca taggcttcgc ccgcggcgcc cgcaccggcg tcatgagcac
       61 cttgatcgcc tcggcggcga agacggcgtc gaacgcggcg gtgaacttgg tgtcacgatc
      121 ccggaccagg aaccggaacc ggccggcgcg gtcgtcaagg gtcatgagca ggttgcgggc
      181 ctgctgggtc agccactccc ccactgggtg cgccgtcacc cccagcacct ggacctggcg
      241 ggtggcgacc tcgatcacaa acagcacgta caggcgtttg agaaacaggt gtccacggtg
      301 aagcaggtcc accgccagca cacccttggc ctgcgcctgg aggaactgcc gccaggtcag
      361 ggccgaccgc ttgggtgagg ggtcaactct ggcgcgctgc agaatcgtcc acacggtgct
      421 ggccccgatg cggtagccga ggcggcacag ctcgccgtgg atgcggcggt agcccaggtc
      481 gggttctccc tggccagccg cagcaccaga ccgcggagct ccagcgccac gcctggacgg
      541 ccacgtcggt gtgggcaggt ccaccggcgc cggaccaaat cttgatgcca gcgcagcagc
      601 gtctcgggcc tgacgaacaa cctgttccag ctcgggcggg gcagcatccg cgaaagtgcg
      661 gccagcaccg cacggtcagc ccagtccaat cggggtcggg tcacctgccg gcggagcacc
      721 gcaacctcgt gccgcaacac cagcagttcc gcgttgttgg tcgctgagcg ccgtgccagc
      781 agcaccagcc atcccagcag ctggcagaag atcagataga gcagtcgtag cgccatccct
      841 cgatcatcgg cgtcagcgcc tccctcagcg aagtcgcagc tcagagccta cgaccgactt
      901 tctggcaccc acaagtttga cgcttgagct tggccaggat ctggtcggcg tccctggtcc
      961 aggtgaaggg acgacaatgc ctcctcggat ccggtcaagg gctcatgtgt aagctcgccg
     1021 accaccacca aggcatgccg gcgtcgcggg tgatgtggac caattccaag ggtcacgtca
     1081 gatcgaccac atcctggtca gccgcgagct gttgttcacc gtcactcggc tgctcaacga
     1141 cggcaaggac ctagcctctt gctcaggccc cggccagcag ctcggccgcc ctagccgcgg
     1201 tggcgaggcc gaccccctcg cgggagcctt cggcggtggg gtcatgaccg gccagctggg
     1261 tgccgatgac gcgcacattg cccagtacgg acgcgcccgc gcggtcgacc gggcggagct
     1321 cgcggttcac cctgacgccg gcctgggcca gccgatgcga gccgaagaag tccgcggcca
     1381 ggaactcggt acggtgagcg aagccatcga tgggcaaccc aagcactgtc tcggccaggg
     1441 tgccgtcgcg gtaggtctcg atccccccgc cggccacgcc ccccgtcgcc aggaccgctt
     1501 cgtcgcacgg gatacggacc gggcccgagg cgccctcgcc gaggatggcc gtaacccggt
     1561 cgccgtcgcg ctcggcccca atcgccggga gcccgagccg ccagcccaca ccgagctcgc
     1621 ttagccgctc tcgccaggcg tcgaacagcc gcagccccgg aatcgacggt ggcggcaggg
     1681 ccgcctcaac cacccggagc ccggccagct cttggaggtc ggcccacgcc tcggcggcgt
     1741 tggcgagccc gatcacggcc gggaccacgc acacgtcgac gccagcggcg gcgcggccga
     1801 gcgtgccggc caccgccccg cggaactccc tcccgtcaaa ggcgcgggcc agctcgaccg
     1861 cgttgacatg gcgtccgcgg aatccctggg gcagcgggat ctcgatcggc acggcgtcca
     1921 ggccgatccg gtgcagccgg tcggcgcaca ggtgggacga gaagtcctcg aagccggcaa
     1981 agccgatggc ggcgacccgc ccggcgaggg cgccgtcgct ctgggtctgg agcagcgcgg
     2041 tggtgcgccg ggtcccgatg cccgtgatct ggctgcggtt gacggccggg tcgccgacca
     2101 gctcgatgcc ggcccggtcg gtcagcgccc gcacgtgccc gatcccagcc agcagcgcgc
     2161 cctcgccgac cagcgagtac gggtgcccgt cgggtagccg gccgagtgct tccaaaggac
     2221 gttccaccgg ttccccggcc cccgcctcgt ccccggccgg caggcgcccc agcacgtcga
     2281 tcccgccggc ggcccagtgc gtaaagccgt gaccgcgcgc gatcacctgg acctgccggc
     2341 ctgcctcggc cagcatggcc gctgccgcca gcccggacgg gccggccccg atgaccacga
     2401 cgtcggcgcc catcaccggc ccccttcggt cggctccggg agcggcccgc gggggccgcc
     2461 gggcggcccg gcgtcggtgg cccggcgccc ctcggtgtcg ccggtcaggc cctccccgcc
     2521 gaccccacgc cccgggccgg cctggcgcgg cccagaccag tcgtcgcccg tggcggccgc
     2581 ctggacctgc tcgtcctcgc ccggctcccg ctccaccgtg tccagggcga ggatgccgcg
     2641 gtagatcgcg tcgttgcgca gcgcctggac cgccgcctgg ccccaggcca ccgctcgcag
     2701 cccgcgccag cgctcctgca tgaacaagcg caggttggcc agcgggtcac cgacgtccgg
     2761 ccgcctggcc gcgacgacgg cggcggcacg ccaaccgcac ccgccgccct gggtaggacc
     2821 gaagccgagc cggtactggc ggcgcaggtc gtcaagctgg gtcacgccgg ccccgacggc
     2881 ctcctcgatg gtgcggcggg tgatcagctc gcactcgcag gccagcgggc cgtcggcctg
     2941 cccggcagcg gtcgacgggc gggccagcgg gcgggtcggc tgacggatgc cctgctcggc
     3001 gccgggcagg ggcgtgatcg cggtcgtgca gggacgctcc tcgccacgct tggcgaccat
     3061 gacgtccacg accttctggg ccatctgcct ggaagtggtg agcttgccac ccacgatcga
     3121 cagcaagttg ggcactccat ccccgggctc gtggtccaag acggtgaagg tgcggctgac
     3181 gctgcggccg tgcccgcccg cttcgccctg gaccgggtcg tacaggggtc ggtagccgct
     3241 gtaggcgcgc agcacgcggc cgttggcgat ggcgggcacc agcttggctg tctggtcgat
     3301 gatcaggtcg atctccagcg gggcgacgct gggatcgtcg gcgtcgtcgg cctcggtcga
     3361 tgtggtccca aggatcgccg cgtcgtaggc cggcacgatg atgtcgccgt cggtcggcat
     3421 gcggcagtgg gtgaccagcg agcgcacgta gcggccggcc atcacgacca tggcgccctt
     3481 gtccgggacc atccggaacg gggccccggc catggcacca atccgcccgg cccaggcccc
     3541 ggccgcgttg atcacccagc ggcagccgat ggtccggccc ctgccgccga ccaggtcatg
     3601 gacgcggacc gcgctgaccc gaccgtcgcc gtcgcgctcg aagccgacga ccgggtggtg
     3661 caccaggacc cggccgcctc tggcctcggc gccgtaggcc attgaccaca gcagcctcca
     3721 ggcgtccatg ctgccgtcgg tcggcaccca aaaggcccgc tcgatccggt cggtaagggc
     3781 cggctcctcg cgacgggccg cggccgggtc gacctcctcg cacggcacgc cggtctcggc
     3841 acagccggtg acgaaacggt cgccgtagcc cagatcgtgc tcgggcaaca ggatgaagaa
     3901 gccgcctgtg tcctcgatgg tatgcggcgc gatccggcgc aggacggcgt tctcctcgac
     3961 gcactcgcgg gccgacggca ggtcgttgac cgcgtaccgg gcgcccgaat gcagctcgcc
     4021 gtgccagcgc cccgatgtcc ccgtccccag gtcggagcgg tccaccaaga ccgcctcgat
     4081 accgcgcaag gccaggtccc acaacacggc cgtcccggtg gcgcccccgc caatcaccac
     4141 gacgtcggtc tcgcgcagca tcgcctgctc ccttcgcggc cagcgcccgt cgtaccaagc
     4201 gtactgccgc gctgggggtg gtcggccacc ttcgggacca aaccggcgcc cccgccgggg
     4261 cccatgccgg cccgccgccc cgtccaagcc gccgtcgacc ggcgcttcaa ccggtgccgc
     4321 tacaacgccg cccgcacgat ccaggcgttc agcgcccggc tacgccagca gatcgagctg
     4381 gacacctgac cggcgagcgg ttcaccgtgc tcgaccagac gatgcagccg ataatgccct
     4441 cgccgcccat cagatgattc cagcaacgca aggctgacgc agggtgccgg agggattcga
     4501 cgaactggcc atggccatct gatcgggcgg aggttgaggc tccagcaagc ccttggtcgc
     4561 cgcaatgcaa caacgccggc ggactcggtt gctgcctcac tgcggtt
//
LOCUS       DASSWR010000181         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_770746,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000181 DASSWR010000000
VERSION     DASSWR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770746"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFO47_02965"
     CDS             complement(<1..336)
                     /locus_tag="VFO47_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET8787692.1"
                     /translation="MTAVVAPEVGARQYETVIGIETHCELGTATKMFCACPTVFGAEP
                     NTQVCPVCLGEPGSLPVANGRAVEFAARIGLALHCRIAERSQFHRKNYFYPDMPKNYQ
                     ISQYDEPLCV"
     gene            358..2649
                     /locus_tag="VFO47_02970"
                     /pseudo
     CDS             358..2649
                     /locus_tag="VFO47_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            2646..3044
                     /locus_tag="VFO47_02975"
     CDS             2646..3044
                     /locus_tag="VFO47_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8787693.1"
                     /translation="MTRRTASTRRRRRDAGTGKGADGNGGRPRVVVVGGGFAGLSAVR
                     ELGGADVEVLLLDRDLYSTFQPLLDQVATGGPNPGDVTYALRAFAARHPNAQFRRMAV
                     SGVDAERRCVRTDDGEWPTSRCGTWPGRVG"
BASE COUNT          412 a         1100 c         1121 g          420 t
ORIGIN      
        1 gacgcacagc ggctcgtcgt actgggagat ctggtagttc ttgggcatgt ccggatagaa
       61 gtagttcttg cggtggaact gggaccgctc ggcgatccgg cagtgcaggg ccagcccgat
      121 gcgggccgcg aactcgacgg ctcggccgtt ggccaccggc agcgaccccg gctcgcccag
      181 gcagacgggg cacacctggg tgttgggctc ggccccgaag acggtcgggc aggcgcagaa
      241 catcttggtc gcggtgccca gctcgcagtg ggtctcgatg ccgatgacgg tctcgtactg
      301 gcgggcgccg acctcggggg cgacgacggc ggtcacgggc gcacctcctg gggccgggtc
      361 accggcgaga gcgagccggt gctcaaggac accgcgaccc tgccggcccc ggcgccgctc
      421 ggggaccgga ccaacatggt gttcagcggc acggccgtga cccggggacg gggccaggcg
      481 gtggtgaccg cgaccggcat ggccaccgag atgggccgga tcgccgccct gctcggccgc
      541 accaaggaag agcgcacccc cctgcagcgc gaggtcgacc tggtcggccg cgtgctcggc
      601 atcgccgtgg tcgccatcgc ggtcgtggtc atggcggcca tcctggtgac ctccgacatc
      661 cggcagccga gcgacctggt cgaggtcctg ctgctgggcg tctccctggc cgtggccgcc
      721 gtccccgagg gcctgccggc ggtgctgtcg gtcgtcctcg ccctcggcgt ccagcgcatg
      781 gccgccaagc acgccatcgt caagaggctg tcgtcggtgg agaccctggg ctcggcctcg
      841 gtcatctgct cggacaagac cggcaccctg accaggaacg agatgaccat ccagcgggtg
      901 gtgacccgtt ccggcgaggt gacggtgacc ggcatcggct accggtccga gggcgacctg
      961 cgggccgacg gccatcccct ctcggacggt ccgttgctgg acgaggtccg ggccgtgctg
     1021 agcgggggca gcctggccaa cgacgccgtc ctgggcgagg acgacgacgg ccagtggagg
     1081 atccagggcg accccaccga cgccgccttc ctggtcgccg agcgcaagct cggcatcacc
     1141 gagcagcgcc gggcccgttt cgagcgcgtc ggcgaggtgc cgttcacctc cgagcgcaag
     1201 ctcatgacca gcatcgaggt cgacgccggc cgggagggtc gcatcgccgt ggtgaccaag
     1261 ggggcccccg acgtgctgct ggcccgctgc acctccgaac gggtcgggga ggccgaggcg
     1321 gccctgaccg aggcccgccg ggccgagatc ctggccgtgg tcgaccgcct ggccgaggcg
     1381 gccctgcgca ccctggccgt cgcctaccgg cccctgaagg ccaccgagcc gccggccgcc
     1441 gaggagtccc tggagcagga gctggtgttc gccggggtgg tcggcatcat cgacccgcca
     1501 cgcccggagg cggccgcggc catcgccgaa gcccacggcg ccggcctgcg ggtcatcatg
     1561 atcaccggcg accatccccg caccgccgcc cggatcgccg ccgacctggg catcgccgag
     1621 cccgaggtcg gccaggacag cgtcccctcc ctcagcgggc tggagctgga cgccctcgac
     1681 gacgaggcgt tcgccgagcg ggtcaggtcg gtgtcggtct acgcccgggt cgcgcccgag
     1741 cacaagctgc ggatcgttga cgccctccag gccgacggga acatcgtcgc catgaccggc
     1801 gacggcgtga acgacgcccc ggccctgaag tcggccgaca tcggcgtggc catgggcgtc
     1861 gccggcaccg acgtcaccaa ggaagcggcc aagatgatcc tggccgacga caacttcgcc
     1921 accatcgtgg ccgccgtgcg cgaggggcgg gccatcttcg ccaacatccg caagttcctg
     1981 cgctatctgc tgtcgtccaa catcggcgag gtgctgacga tgttcctcgg cgtggtcggc
     2041 gcctcggtga tcggcctgga cgtcgccggc gggacggtgg tggcgcccct gctggccacc
     2101 cagatcctct ggatcaacct gctcaccgac accggcccgg cgctggccat gggggtcgac
     2161 ccgccaccgg gggacgtgat gcgccacccg ccccgcaggc tcaccgaccg ggtcatcgac
     2221 cgggagatgc agctcggcgt ggtcttcgtc gggctggtga tggcgctggt caccctggcc
     2281 acgatcgacc tcaagctgcc cggcggcctg ttcgaggggt cggccgggct gaccgaggcc
     2341 cgcacggccg gcttcaccgt gctggtgctg gcccagctgt tcaacaccgt caactcgcgc
     2401 tcggaacggg tcagcgcctt gcgcggcctg ttcaccaacc ggtggctgtg ggcggccatc
     2461 gccctctcgc tggccctcca gctcctggtc gtgcacgtgc cgctgctcaa ccgggccttc
     2521 ggcaccacgc cgctgtcggc cggcgactgg gcgctgtgcg cggtcatggc cagcgcggtg
     2581 ctctggaccg acgagctcaa gaagctggtc gtccgacggg tggcgcgccg gaaggagagc
     2641 cagcgatgac tcgccgcacg gcatcgaccc gccgacgaag acgggacgcc gggaccggga
     2701 agggggcgga cggcaatggc gggcggcccc gcgtcgtcgt ggtgggcggc gggttcgccg
     2761 ggctgtcggc ggtccgggag ctcggcggcg cggacgtcga ggtgctgctg ctcgaccgcg
     2821 acctgtacag caccttccag ccgctgctgg accaggtggc gaccggcggg ccgaaccccg
     2881 gcgacgtcac ctacgcgctg cgggccttcg ccgcccgcca tcccaacgcc cagttccgtc
     2941 gtatggccgt gagcggggtg gacgccgagc gccgctgcgt ccgcaccgac gacggcgagt
     3001 ggccaacctc tcggtgcggt acctggcctg gccgggtcgg ctgaacgtga tca
//
LOCUS       DASSWR010000182         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_6178,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000182 DASSWR010000000
VERSION     DASSWR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6178"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFO47_02980"
     CDS             complement(<1..182)
                     /locus_tag="VFO47_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787694.1"
                     /translation="MRDQVWDRPRVRGRDHREGLRHLRLLPGKVRDAAAQAIKGPIAA
                     ASSTGRADDSNDVRQRT"
     gene            complement(201..638)
                     /locus_tag="VFO47_02985"
     CDS             complement(201..638)
                     /locus_tag="VFO47_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787695.1"
                     /translation="MTSSDPAAADLERSSERWIAWLAAETAGRRHPPASALHALWDAL
                     EEWFASDDFAGSALAAAVVAPPDGRGPAAHAALVRHRQATRRLLEDLARAAGARDPAA
                     LAGQLLTLVEGAIAGALIDRHPSAARHARELTQIALTAAGPQG"
     gene            807..1571
                     /locus_tag="VFO47_02990"
     CDS             807..1571
                     /locus_tag="VFO47_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787696.1"
                     /translation="MRRQPLPSTLPSRLLRVAVVLGTAVVLAALVAAPASAHTDKKAG
                     KYTFVVGFGEEPAYAGAPNSLQVIISRGGKPATDLAGQLDKLKAHLYYGGKVDPKAEM
                     VELPLEPHFGDGWGTPGDYRSFFVPTQAGAYTVHLNGALGTQKIDLAIPSGPETFSDV
                     NDPAKAAFPAVKDPTTAQLAERLDREATRLTGTMAAAAAAQQEARDTADQARLLALGG
                     LIVGAVGLIVGALAWGRRTSTPAAQPDVALTGAGKV"
     gene            1579..3300
                     /locus_tag="VFO47_02995"
     CDS             1579..3300
                     /locus_tag="VFO47_02995"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET8787697.1"
                     /translation="MRRRGRLAALAPLAGLWLLASGPVAAAHGVLEASEPAGGSSLER
                     APAAVTLRFSERPDPGLSTVRVLDSGGRVVAGGPAQPVAGRPLELRVPAAGLAAGSYT
                     VTWRIVSAVDGHRTDGVFGFGVGAAGAPRMAPSQSAAAVEAGPALGAVAVTGRWAWYW
                     GVALLVGAAATGLLVFGGRLPGRPALLLGAALAAAAGGLAAMAVAARADAGVGLGDML
                     GSTTGGWLLWRAAMLAAAGAAVWWLLARPATDPPLLAPAATEGPRPRRPATVGPLLAI
                     GLAGGAGMLVHALAGHAAGPSSLRPLNLLAQWTHLLGVGVWIGGLAWLFAGLRRGATA
                     RPAREVVLRFSKLAGISLAVVVVTGVVRTADELGGWGRLVDSRFGRALDLKLVLFAGL
                     VGLGALNRYRLVPLFQAGARRRAATRLRRSVGAELGLASAVLAAAALLSQLAPGAPDG
                     ARGAPAATPVLEARGADWATTVKVTLTVTPGAAGPNRFTATVADFDTGAILPADRVEL
                     TGTPASHPVLAGARLELAEAGDGRWLGQGRLLSLAGRWNLTTTIQRPTGGITVPLALE
                     VPPPAPA"
     gene            3456..>3636
                     /locus_tag="VFO47_03000"
     CDS             3456..>3636
                     /locus_tag="VFO47_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787698.1"
                     /translation="MLVPLHRAVSAVLHVMLVAFVTLSARVQHGVLRSKHWFAPATAV
                     AAYALVGLTGWLWLVG"
BASE COUNT          400 a         1395 c         1362 g          479 t
ORIGIN      
        1 gtcctttgac ggacgtcgtt ggagtcgtca gcgcggccgg tggacgacgc ggcggcgatg
       61 gggcccttga tcgcctgggc cgccgcgtcg cggaccttgc ccggaagcag gcgcagatgg
      121 cggagcccct cgcggtgatc tcgacctcga acacgaggcc ggtcccacac ctggtcgcgc
      181 acggggacgg tgaggcgacc ctagccctgg ggacccgcgg ccgtcagggc gatctgggtg
      241 agctcgcggg cgtggcgggc ggcgctgggg tggcggtcga tcagggcgcc ggcgatggcg
      301 ccctcgacca gggtgaggag ctggccggcg agggcggcgg ggtcgcgggc gccggccgcg
      361 cgggccaggt cctcgagcag gcggcgggtg gcctggcggt gccggacgag ggccgcgtgg
      421 gcggccgggc cgcggccgtc gggcggcgcg accacggccg cggccagcgc cgacccggcg
      481 aagtcgtcgg aggcgaacca ctcctcgagt gcgtcccaga gcgcgtgcag ggccgacgcc
      541 ggcgggtgcc ggcgcccggc cgtctccgcg gccaaccagg cgatccagcg ctcgctggac
      601 cgctccaggt ccgccgccgc ggggtcgctg ctggtcatgg cactagctcg ttcccacgct
      661 gatggggcca atcgtactct cctccccatc ctctacatac gggcgaaccg ttcaagactc
      721 gacggtaact agtcatactt gactagtgat aacgttctct ctcattatgc actcatgacg
      781 gtcatgtcgg tgaaaaggag gcaaaaatgc gccgacagcc actcccctcg accctgccaa
      841 gccggttgct gcgggtggcc gtcgtgctcg gcacggccgt ggtgctggcc gcgctcgtcg
      901 ccgcgccggc ctccgcccac accgacaaga aggccggcaa gtacaccttc gtggtcggct
      961 tcggcgagga gcccgcctac gccggggcgc ccaacagcct ccaggtgatc atcagccgcg
     1021 gcggcaagcc cgccaccgac ctggccggcc agctcgacaa gctgaaggcc cacctctact
     1081 acggcggcaa ggtcgacccc aaggccgaga tggtcgagct gcccctggag ccgcacttcg
     1141 gcgacggctg gggaacgccg ggtgactacc ggtcgttctt cgtcccgacc caggccggcg
     1201 cctacaccgt ccacctgaac ggcgcgctcg gcacccagaa gatcgacctg gccatcccga
     1261 gcggccccga gaccttcagt gacgtcaacg acccggccaa ggccgccttc ccggccgtca
     1321 aggaccccac cacggcccag ctcgccgagc ggctcgaccg tgaggcgacc cggctgaccg
     1381 ggaccatggc ggcggccgcg gccgcccagc aggaagccag ggacacggcc gaccaggccc
     1441 gcctgctggc cctgggcggg ctgatcgtcg gcgccgtcgg actgatcgtc ggtgccctgg
     1501 cctggggacg gcggacgtcc accccggccg cccagcccga cgtcgccctg accggcgccg
     1561 ggaaggtctg acggtcccat gcgacgccgt gggcggctcg ccgcgctcgc tccgctggcc
     1621 gggctctggc tgctggccag cggtccggtg gcggccgccc acggcgtcct cgaggccagc
     1681 gagccagccg gcgggagctc gctggagcgg gcgccggcgg cggtgaccct ccgcttcagc
     1741 gagcgtcccg accctggcct gtccacggtg cgggtgctcg acagcggcgg acgggtcgtc
     1801 gccggcggcc ccgcgcagcc ggtcgccggc cggcccctgg agctgcgggt cccggcggcc
     1861 gggctcgcgg ccggcagcta cacggtgacc tggcggatcg tctcggccgt cgacggccac
     1921 cgcaccgacg gggtcttcgg gttcggggtc ggcgcggccg gcgccccccg gatggcgccg
     1981 tcccagtcgg cggccgcggt cgaggccgga ccggctctcg gcgcggtcgc cgtgaccggc
     2041 cgctgggcct ggtactgggg ggtcgccctg ctggtcgggg cggccgccac cggcctgctg
     2101 gtcttcgggg gccgcctgcc ggggcggccg gcgctgctcc tgggcgcggc cttggcggcc
     2161 gcggcgggcg ggctggccgc gatggcggtg gccgcccggg ccgacgccgg ggtgggcctc
     2221 ggcgacatgc tcggctccac caccggcggc tggctgctct ggcgggcggc catgctcgcc
     2281 gccgccggcg ccgccgtctg gtggctgctg gcccgcccgg cgaccgaccc cccgctcctg
     2341 gccccggcgg ccaccgaggg cccgcgcccc cgccgccctg ccaccgtcgg cccgctgctg
     2401 gccatcgggc tggccggcgg ggccgggatg ctggtgcacg cgctggccgg gcacgcggcc
     2461 ggcccgtcgt cgctgcgccc cctgaacctg ctcgcccagt ggacccacct gctgggcgtc
     2521 ggcgtgtgga tcggcggcct ggcctggctg ttcgccggcc tccggcgcgg cgccacggcc
     2581 cggccggccc gggaggtggt gctgcgcttc tccaagctcg ccggcatcag cctggccgtg
     2641 gtggtggtca ccggcgtggt ccggacggcc gacgagctcg gcggctgggg gcggctggtc
     2701 gacagccggt tcggccgggc cctcgacctc aagctggtcc tgttcgccgg gctggtgggg
     2761 ctgggggcgc tcaaccgcta ccggctggtg ccgctgttcc aggccggcgc ccggcgccgg
     2821 gccgcgaccc ggctgcggcg cagcgtcggc gccgagctgg ggctggcgtc ggctgtgctg
     2881 gccgccgccg ccctgctcag ccagctcgcc ccgggcgccc cggacggggc ccggggggcg
     2941 ccggcggcca cgccggtcct ggaggcccgt ggggccgact gggccaccac cgtcaaggtc
     3001 accttgaccg tgaccccggg ggcggccggg ccgaaccgct tcaccgcaac cgtggccgac
     3061 ttcgacaccg gcgcgatcct gcccgccgac cgggtcgagc tgaccggcac gcccgcgtcc
     3121 caccccgtcc tcgccggtgc ccgcctcgag ctggccgagg cgggcgacgg ccgctggctg
     3181 ggtcagggcc gcctgctgtc cctggccggc cgctggaacc tcaccaccac catccaacgc
     3241 cccaccggcg gcatcaccgt gcccctggcc ctggaggtcc cgccccccgc tcccgcctga
     3301 cgccccaacg ggcggacccg gaatgtcgct cgttccggaa ttcgccccct atgaatgaga
     3361 gagaacagtc actatcattt gtccagatca atgtggttcg cccggcgagg gaggcgccgg
     3421 atgatcgaca ggatcgaccg agaagggagc atcccatgct cgttcccctc caccgggcgg
     3481 tgtcagccgt cctccacgtc atgctggtcg ccttcgtgac cctgtccgcg cgggtccagc
     3541 acggcgtgct ccgctcgaag cactggtttg cgccggcaac cgctgtggcc gcctacgccc
     3601 tcgtcgggct caccggctgg ctctggctgg tcggcc
//
LOCUS       DASSWR010000183         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_120757,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000183 DASSWR010000000
VERSION     DASSWR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120757"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..728
                     /locus_tag="VFO47_03005"
     CDS             9..728
                     /locus_tag="VFO47_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787699.1"
                     /translation="MDEALLDVKDIHVFYGNIEAVKGMSFHVNRGEIVCLIGANGAGK
                     TTTLRTVSGLLRPRVGAIFYEGQRIDLVPAHEIVGLGVAQSPEGRRIFPRMSVRENLD
                     MGAFIRRDADAQREDLERIMELFPILKERARQAAGTLSGGEQQMLAMGRALMARPKLL
                     LLDEPSMGLAPLVVQTIFDTIRDINSQGMTVLLVEQNAAQALSLAHRGYVIETGKIVL
                     EDEASALLENEQVRKAYLGEE"
     gene            1140..1571
                     /locus_tag="VFO47_03010"
     CDS             1140..1571
                     /locus_tag="VFO47_03010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787700.1"
                     /translation="MADTLNLTLPPSSSCLPDLRRSVARTLEGVDEEVVADVLLALDE
                     AVSNAIRHGSRAGDPVLVSVKADGGWIEMVVHDGGPTPRLPRLPAEPPPALQTGGRGL
                     WLILQLVDEVRLQRIDGGTRLVMRRQVSSRVDSLVGPDGGG"
     gene            1585..>2381
                     /locus_tag="VFO47_03015"
     CDS             1585..>2381
                     /locus_tag="VFO47_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET8787701.1"
                     /translation="MATSEPPTPSPLVLLDGHSLAYRAFFALPSDLQTTTGQLTNVVY
                     GFTTMLVKLFGDFSPDRIAVCFDLGRPAYRHDVYDGYKANRRTTPDDFSSQMPLVREV
                     LAALRIPVVEVAGYEADDLIATLVREARDESLPVLVVTGDRDNLQLIDDGADVRVMMT
                     RRGITDTAIYDEAAVQERYGVPPGRYIDVAALRGDPSDNLPGVPGVGDKTATKLVQTY
                     GTAEEVVAHADEQRGKLKDNLLAHGDDVLRNKQLMRLRDDVPLPVGVR"
BASE COUNT          363 a          878 c          807 g          333 t
ORIGIN      
        1 cgtcgtagat ggacgaggcg ctgctcgacg tcaaggacat ccacgtcttc tacggcaaca
       61 tcgaggccgt caagggcatg tccttccatg tcaaccgggg cgagatcgtc tgcctgatcg
      121 gcgccaacgg ggccggcaag acgaccaccc tgcgcaccgt ctcggggctg ctgcggccac
      181 gcgtgggggc gatcttctac gagggacagc ggatcgacct ggtgccggcc cacgagatcg
      241 tcggcctggg ggtggcccag tcgccggagg gccggcgcat cttcccgcgc atgagcgtcc
      301 gcgagaacct ggacatgggc gccttcatcc gccgcgacgc cgacgcccag cgcgaggacc
      361 tggagcggat catggagctg ttcccgatcc tcaaggagcg ggccaggcag gccgccggga
      421 ccctgtccgg gggcgagcag cagatgctgg ccatgggccg ggcgctgatg gcccggccga
      481 agctgttgct gctcgacgag ccgtcgatgg gtctggcccc gctggtcgtc cagaccatct
      541 tcgacaccat ccgcgacatc aactcccagg gcatgaccgt cctgctggtc gagcagaacg
      601 ccgcccaggc actcagcctc gcccaccgcg gctacgtgat cgagaccggc aagatcgtcc
      661 tcgaggacga ggccagcgcg ctgctcgaga acgagcaggt ccgcaaggcc tacctggggg
      721 aggagtagcc ccggagccga cggccggcct gtccggcggg ggccgagagc ctttggctcc
      781 gttcgcgcac cttccccgag tgcgcgcgtt ctctactcgg tcctcggacc cccgcgccgg
      841 acggacctcg gggcccccgt ggggaccccg attgcgggca cgctaacagc cgggcgggca
      901 gagggtcaac cggaccattc ccttgcggcg caacgggtcc agcatcgttg caggtcgcac
      961 ctgcgacgat gagttccgag cctccgcgcg gtttcctccc acctcgctcg ccccgggtga
     1021 tgtcccggcg ggcaggagtc taggtcccga tccggacgag atcctcgctc gggcggtttg
     1081 aggccgtcgg cgaccggggt aggaggtgac cgtcccgacc tgagcacacc gacgagcaaa
     1141 tggcggacac cttgaacctg accctgccac ccagttcctc ctgccttccc gaccttcggc
     1201 gctcggtcgc ccggaccctg gagggtgtcg acgaggaggt ggtcgccgac gtgctcctgg
     1261 ccctcgacga ggcggtcagc aacgccatcc gccacggctc gcgggccggt gacccggtgc
     1321 tggtgtcggt caaggccgac ggcgggtgga tcgagatggt cgtgcacgac ggcggcccga
     1381 ccccccgcct gccccgcctg ccggccgagc ctccgcccgc cctccagacc ggcggccgcg
     1441 gcctgtggct gatcctccag ctggtcgacg aggtccggct gcagcggatc gacgggggca
     1501 cccggctggt catgcgacgc caggtcagct ccagggtgga cagcctggtc ggccccgacg
     1561 gaggcggttg atagactcgg ccgcgtggcc acctcggaac cgccgacccc atccccgctg
     1621 gtcctgctgg acgggcacag cctcgcctac cgggccttct tcgccctgcc cagcgacctc
     1681 cagacgacca ccgggcagct gaccaacgtc gtctacggct tcaccaccat gctggtcaag
     1741 ctgttcggcg acttctcccc ggaccggatc gcggtctgct tcgacctggg ccggcccgcc
     1801 taccgccacg acgtctacga cggttacaag gccaaccgcc ggaccacccc cgacgacttc
     1861 agctcccaga tgccgctggt ccgcgaggtg ctggccgccc tgcgcatccc ggtggtcgag
     1921 gtcgccggct acgaggccga cgacctgatc gccaccctgg tcagggaggc gagggacgag
     1981 tcgctgccgg tgctggtcgt caccggggac cgggacaacc tccagctgat cgacgacggg
     2041 gccgacgtcc gggtgatgat gacccggcgc ggcatcaccg acaccgccat ctacgacgag
     2101 gccgccgtcc aggagcgcta cggcgtgccc ccaggccgct acatcgacgt ggccgccctg
     2161 cgcggcgacc cctccgacaa cctgccgggc gtccccgggg tcggcgacaa gacggccacc
     2221 aagctggtcc agacctacgg aaccgccgaa gaggtcgtgg cccacgccga cgagcagcgg
     2281 ggcaagctca aggacaacct actcgcccac ggcgacgacg tgctccgcaa caagcagctg
     2341 atgcggctgc gcgacgacgt gcccctgccc gtcggggtcc g
//
LOCUS       DASSWR010000184         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1109350, whole genome shotgun sequence.
ACCESSION   DASSWR010000184 DASSWR010000000
VERSION     DASSWR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109350"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFO47_03020"
     CDS             complement(<1..273)
                     /locus_tag="VFO47_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787702.1"
                     /translation="MAYRFTPADRGAFKRCRRQWDFGARERQNLEPVEAVAGPDLELA
                     VRDALAVYYFPGMWDWQRSVVLPLVVQGLARSAGRGPPAGEQELAAA"
     gene            346..927
                     /locus_tag="VFO47_03025"
     CDS             346..927
                     /locus_tag="VFO47_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8787703.1"
                     /translation="MAVSSLMVPLGTPAHDFELPSADGSTVKLADLDGRALLVMFLCN
                     HCPYVRHVERQLGAVVAEYAGKGVAAVGICSNDLDAYPDDGPAGMADQARRAGFEFPY
                     LLDTDQQVAAAYRAMCTPDLFVYDADRRLAYRGAFDDSTPRNGQPVTGALLRDALDRV
                     LAGQPVPEPHRPSMGCSLKWRPGNRPSWLVASG"
     gene            complement(949..2958)
                     /locus_tag="VFO47_03030"
     CDS             complement(949..2958)
                     /locus_tag="VFO47_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HET8787704.1"
                     /translation="MATNPDPFRRIELPVLQVGRRNHKSCRWGCGDACYKEPPNRSGN
                     ETYAAVVERALSRRAFLAAGGVGALVLGAAASPADASEAPPVGPYKPHPTGRRLAFTP
                     IAPNTVDDVVLADGYSNVILIAWGDPIRPGAPEFDFDAQTPRDQARQFGYNNDYLAFF
                     PLPWWSANSFIGLLWSNHEYTNPELMFRNYDAGDPTREQVDIQIQAHGGSIVLIQRAK
                     RGGPFKYRPLSRYNRRITATTPMKLTGAAAGDDLLKTSDDPTGRRVRGMLNNCAGGVT
                     PWGTVLTGEENFHQYFANNALVTDPKVKAWHERYALPDGASDRKWERYHDRFDLTKEP
                     HEPFRFGWVVEIDPYDPHFTPRKHTNLGRLKHEGATTTLTKSKRVAVYTGDDERFEYF
                     FKFVTRDRYRRWDRKHNLGLLEHGTLHVAKLSDDGTGRWIPLRHGENGLTAANGFASQ
                     AEVLINARGAGDTLGATKMDRPEDIQRNPVTGRVYVNLTNNTEREAPDPGNPRSPNPY
                     GHVLELREDGDDADSTSFTWRIFLLCGDPASPDTYFAGYPKELVSPIGSPDNMTFDNE
                     GNLWLATDGLQPGETNNGLFACPTRGEERGHVQLFATVPVGAETCGPLLTQDNRSLFI
                     AVQHPGEGGTVEEPTSHWPDGGDSLPRPAVTSIWRSADGDPTIGQ"
BASE COUNT          401 a         1084 c         1154 g          481 t
ORIGIN      
        1 ggcggcggcc agctcctgct caccggccgg cggcccgcgg cccgccgagc gggccagccc
       61 ctgcacgacc agcggcagca ccaccgagcg ctgccagtcc cacatgccgg ggaagtagta
      121 gacggccagg gcgtcgcgga cggccagctc caggtcaggg ccggcgaccg cctcaacggg
      181 ctcgaggttc tgccgctccc tggccccgaa gtcccactgc cggcggcagc gcttgaacgc
      241 cccccggtcc gccggcgtga agcggtaggc catgtccgct agcgtaggac cgtccccctc
      301 ggaccggaag acgacagggc ccgggcgccc caggagaacc aggacatggc cgtttcctcg
      361 ctcatggtgc cgctcggcac ccccgcccac gacttcgagc tgccgtcggc cgacgggtca
      421 acggtcaagc tggccgacct cgacggccgg gccctgctgg tgatgttcct ctgcaaccac
      481 tgcccgtacg tccgccacgt cgagcgccag ctcggggccg tggtcgccga gtacgccggc
      541 aagggcgtgg ccgccgtcgg catctgcagc aacgacctcg acgcctaccc cgacgacggc
      601 ccggccggca tggccgacca ggcccgccgg gccggcttcg agttccccta cctgctcgac
      661 accgaccagc aggtcgccgc cgcctaccgg gccatgtgca cccccgacct gttcgtctac
      721 gacgccgacc gccggctcgc ctaccgcggc gccttcgacg acagcacccc ccgcaacggc
      781 cagccggtca ccggggcgct gctgcgcgac gccctcgacc gggtcctggc cggccagccg
      841 gtgcccgagc cgcaccgccc cagcatgggc tgcagcctca agtggcggcc cggcaaccgg
      901 cccagctggc tggtcgcctc cggctgagcc gccccggcgc ggcccggcct actgcccgat
      961 cgtcgggtcg ccgtcggcgc tgcgccagat cgaggtcacg gccggccggg gcaggctgtc
     1021 cccgccgtcc ggccagtggc tggtcggctc ctcgaccgtg ccgccctcgc ccggatgctg
     1081 gacggcgatg aacagcgagc ggttgtcctg ggtgagcagc gggccgcagg tctcggctcc
     1141 caccgggacg gtggcgaaca gctgcacgtg cccacgctcc tcgcccctgg tgggacaggc
     1201 gaacaggccg ttgttggtct cgccgggctg gagcccgtcg gtggccagcc agaggttgcc
     1261 ctcgttgtcg aaggtcatgt tgtccggcga gccgatgggg ctgaccagct ccttggggta
     1321 gccggcgaag taggtgtcgg ggctggccgg gtcgccgcag agcaggaaga tccgccaggt
     1381 gaagctggtc gagtcggcgt catcgccgtc ctcgcgcagc tccagcacgt gcccgtaggg
     1441 gttgggggag cgggggttgc ccgggtcggg agcctcccgt tcggtgttgt tggtcaggtt
     1501 gacgtacacc cgcccggtga ccgggttgcg ctggatgtcc tcgggccggt ccatcttggt
     1561 cgccccgagg gtgtcgccgg cgcccctggc gttgatcagc acctcggcct ggctggcgaa
     1621 gccgttggcg gcggtcagcc cgttctcgcc gtggcgcagg gggatccagc ggccggtgcc
     1681 gtcgtccgac agcttggcaa cgtgcagggt gccgtgctcc agcaggccga ggttgtgctt
     1741 gcggtcccac ctgcggtacc ggtcccgggt gacgaacttg aagaagtact cgaagcgctc
     1801 gtcgtcgccg gtgtacacgg ccacccgctt gctcttggtc agggtcgtgg tcgccccctc
     1861 gtgcttcaac cgcccgagat tggtgtgctt gcgtggggtg aagtgcgggt cgtaggggtc
     1921 gatctcgacc acccagccga agcggaacgg ctcgtgcggc tccttggtca ggtcgaagcg
     1981 gtcgtggtag cgctcccact tgcggtccga ggcgccgtcg ggcagggcgt agcgctcgtg
     2041 ccaggccttg accttcgggt cggtgacgag cgcgttgttg gcaaagtact ggtggaagtt
     2101 ctcctcgccg gtcaggaccg tgccccaggg ggtgaccccg ccggcgcagt tgttcagcat
     2161 tcccctgacc cgccgcccgg tcgggtcgtc gctcgtcttg agcaggtcgt cgccggcggc
     2221 ggcgccggtg agcttcatgg gggtggtggc ggtgatgcgc cggttgtagc gcgacagcgg
     2281 gcggtacttg aacgggccgc cgcgcttggc ccgctggatg agcacgatgc tgccgccgtg
     2341 ggcctggatc tggatgtcga cctgctccct ggtcgggtcg ccggcgtcgt agttccggaa
     2401 catcagctcc gggttggtgt actcgtggtt cgaccacagc aggccgatga agctgttcgc
     2461 gctccaccag ggcagcggga agaaggccag gtagtcgttg ttgtagccga actggcgagc
     2521 ctggtctctc ggggtctggg cgtcgaagtc gaactcgggc gcgcccgggc ggatcgggtc
     2581 gccccaggcg atcaggatca cgttcgagta gccgtcggcc aggaccacgt cgtcgacggt
     2641 gttgggggcg atcggggtga aggcgagccg gcgcccggtc gggtgcggct tgtaggggcc
     2701 gaccggtggc gcctcggagg cgtcggccgg ggatgccgcc gcccccagca ccagggcccc
     2761 gaccccgccc gcggccagga aggcgcggcg gctcagggcc cgctcgacca cggcggcgta
     2821 ggtctcgttg ccggaccggt tgggtggctc cttgtagcag gcgtcgccac agccccagcg
     2881 gcagctcttg tggttgcgcc ggccgacctg gagcacgggc agctcgatgc ggcggaaggg
     2941 atcggggttg gtcgccattg ccacctccga tttgcgggag aatgtccaga gaaacagcca
     3001 agggacgggt tgtccagagc gagatcggcg cgggacgtcg gcgagcgggc accgtgatcg
     3061 acgcggcgcg gaccaggctc cggacgctcc gatccacagt cggacggcac gaaccggacg
//
LOCUS       DASSWR010000185         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1363906, whole genome shotgun sequence.
ACCESSION   DASSWR010000185 DASSWR010000000
VERSION     DASSWR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363906"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFO47_03035"
     CDS             complement(<1..671)
                     /locus_tag="VFO47_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8787705.1"
                     /translation="MSTEVNAGRTVTPAVTSPAPAVRKKSPYPYWFCLPAAVIFVVFF
                     VVPTFSSFFFSLTRWDLTDWEFIGLDNFDNFFQEPALVIGLKNTLIYAVVTCGLKVVL
                     GILLAQLLTSQIIARGFLRSVAFFPVLVSTIGVGLTFTVLMDPSAGVINKSLALVGIE
                     GPAWLVDPKFALLSVALVDVWKGVGLATVIFIAGIVSIPREYFEASAVDGASAFRRFW
                     HIVLPL"
     gene            complement(677..1999)
                     /locus_tag="VFO47_03040"
     CDS             complement(677..1999)
                     /locus_tag="VFO47_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8787706.1"
                     /translation="MRFAKLRRPISLFAATVACILVLGACSAGSLGSSDEGGGTTLTF
                     LVDNAEGSLKPAQALAEAFHAKNPDVTVQVQTRPGGSEGDNIVKTRLSTGEMTDVFLY
                     NSGSLFQALNPRQNLTPLTGEAWAGQVEDTFKPVVSVDNELYGAPITGSSAGGILYNR
                     KVYEELGLQVPKTWAEFMANNAKIKAANIDPVIQTYQDTWTSQLFVLADFHNVAAADP
                     GWAEKYTAGQVKYAQEPAVKCFQRLEEVNKAGYENNYYRSMKFEKGVSDLAAGKGAHF
                     PMLTFAVGTLATSAPDRINDVGFFGQPGDDASKAGMTIWMPAGLYIPKSTEGAQLEAA
                     KKFVAFVASPEGCDVQTKAYPPAGPYMVKGCQLPADVPQAVKDVVAYVEQGNITTALE
                     FQSPIKGPALEQITVEVGSGLRSAADGAALYDQDVKKQAQQLGLKGWS"
     gene            complement(2042..>2590)
                     /locus_tag="VFO47_03045"
     CDS             complement(2042..>2590)
                     /locus_tag="VFO47_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET8787707.1"
                     /translation="LGAVADWLHRTFAGLAPAAPGYRELLVRPEPARSLTSASARHRT
                     PYGDAEVAWQRSEGQLRLRVVVPVGATASVRVPGWPEPVRVGHGTHTWQTADPAAVDP
                     PLAAPATVREVLDHEDTWRQVVAAAVETGVVSDEAQAAARLQAFLDAPSSRLVDALAL
                     APPNLAKGRRALHARLDHLLPP"
BASE COUNT          392 a          868 c          938 g          392 t
ORIGIN      
        1 agcggcagga cgatgtgcca gaaccgccgg aacgccgagg cgccgtccac cgccgacgcc
       61 tcgaagtact cgcgggggat ggagacgatc ccggcgatga agatcaccgt ggccagcccc
      121 acgcccttcc agacatccac caaggccacc gacagcagcg cgaactttgg gtccaccagc
      181 caggccggcc cctcgatgcc gaccagcgcc agcgacttgt tgatgacccc ggccgacggg
      241 tccataagga cggtgaaggt caggccgacc ccgatggtgc tcaccagcac cgggaagaag
      301 gccaccgagc gcaggaaccc cctggcgatg atctgggagg tgagcagctg ggccagcagg
      361 atgcccagca ccaccttgag cccgcaggtg acgaccgcgt agatcagggt gttcttgagg
      421 ccgatgacca gcgccggctc ctggaagaag ttgtcgaagt tgtccaggcc gatgaactcc
      481 cagtcggtga ggtcccagcg ggtcaggctg aagaagaacg acgagaacgt cggcacgacg
      541 aagaacacga cgaagatgac cgcggcgggc aggcagaacc agtaggggta cgggctcttc
      601 ttccggaccg ccggcgccgg cgacgtcaca gccggcgtga cggtccggcc cgcgttcacc
      661 tcggtcgaca tggtggctac gaccagccct tgagccccag ctgctgggcc tgcttcttga
      721 cgtcctggtc gtagagggcc gccccgtccg cggccgaccg cagcccggag ccgacctcga
      781 cggtgatctg ctcgagggcc ggtcccttga tgggggactg gaactccagc gcggtcgtga
      841 tgttcccctg ctccacgtag gccaccacgt ccttgaccgc ctgggggacg tcggccggca
      901 gctggcagcc cttgaccatg tacgggccgg ccggtgggta ggccttcgtc tgcacgtcgc
      961 atccctccgg gctggccacg aaggccacga acttcttggc cgcctcgagc tgggcgccct
     1021 cggtcgactt ggggatgtag agaccggccg gcatccagat cgtcatcccg gccttggacg
     1081 cgtcgtctcc cggctgcccg aagaagccga cgtcgttgat gcggtcgggg gcggatgtgg
     1141 ccagggtgcc caccgcgaac gtcagcatgg ggaagtgcgc gcccttgccc gcggcaaggt
     1201 cactcacgcc tttctcgaac ttcatggacc ggtagtagtt gttctcgtac ccggccttgt
     1261 tcacctcctc aaggcgctgg aagcacttga cggccggctc ctgggcgtac ttcacctggc
     1321 cggcggtgta cttctcggcc cagccagggt cggcggcggc gacgttgtgg aagtcggcca
     1381 ggacaaacag ctgcgacgtc caggtgtcct ggtatgtctg gatcaccggg tcgatgttgg
     1441 cggccttgat cttggcgttg ttggccatga actcggccca ggtcttgggc acctgcaggc
     1501 ccagctcctc gtacaccttc cggttgtaga ggatgccgcc ggcggacgag ccggtgatcg
     1561 gagcgccgta gagctcgttg tccaccgaca ccacgggctt gaaggtgtcc tcgacctggc
     1621 cggcccaggc ctcgccggtc agcggggtca ggttctgccg ggggttgagg gcctggaaca
     1681 gggagcccga gttgtacagg aagacatcgg tcatctcgcc cgtggagagc cgcgtcttga
     1741 cgatgttgtc gccctcgctg cctccggggc gcgtctgcac ctggacggtg acgtctgggt
     1801 tcttggcgtg gaaggcctcg gcgagggcct gtgccggctt gaggctcccc tcggcgttgt
     1861 cgacgaggaa ggtgagcgtc gtcccgccgc cctcgtcgct ggagccgagg ctcccggcgc
     1921 tgcaggcgcc gagcaccagg atgcaggcca ccgtggcggc gaacagcgag atcggccgcc
     1981 ggagcttggc gaacctcatg agcgtacctc cttgggcttg aaccgatcgc gagcggggcg
     2041 gctatggcgg caggagatgg tccagccggg catggagggc ccggcggccc ttggccaggt
     2101 tcggcggcgc cagggcgagg gcgtcgacca gccggctcga cggcgcgtcg aggaaggcct
     2161 ggagccgggc ggcggcctgt gcctcgtcgc tgaccacgcc ggtctccacg gcggcggcga
     2221 ccacctgccg ccaggtgtcc tcgtggtcga ggacctcgcg gacggtcgcc ggggccgcca
     2281 gcggggggtc gaccgccgcc gggtcggccg tctgccaggt gtgagtgccg tggccgacgc
     2341 gcaccggctc cggccacccg gggacgcgca cgctggccgt ggcgccgacc gggacgacga
     2401 cccgcagccg gagctggccc tcggaccgtt gccaggcgac ctcggcgtcg ccgtagggcg
     2461 tgcgatgacg cgccgaggcc gaggtcaggc tccgggccgg ttccggacgg acgagcagct
     2521 cgcggtagcc gggcgccgcc ggggcgaggc ccgcgaacgt gcggtgcagc cagtcggcca
     2581 ccgcgcccag
//
LOCUS       DASSWR010000186         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_911331,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000186 DASSWR010000000
VERSION     DASSWR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911331"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFO47_03050"
     CDS             complement(<1..1104)
                     /locus_tag="VFO47_03050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8787708.1"
                     /translation="MRVGGQGTLEAAAERLAAFAGRRVALPGLAVGLIGPGGWRRELA
                     VGVTDVASGRPLAREALMPVASVGKAMTAAALLREHQAGRLDLDAPVHEHLPWLPLAT
                     PFGPISVRHLLGHTAGIVTGMEGSPSPYLEALALAHTPPGWPPGEKTRYSNVGYAVLG
                     LVLERVAGSSYAEAVRRHVLDPCGMAGSEAVTTAEAQARAATGHVRLAGGAMVPAPWV
                     PTAAGSGATLCTAPDLGRFLRAVVAGDPPLLGPAAREAMLTPALPIPDGGYGYGLGVE
                     LDDQDGYRRVGHQGDCPGYCSYAFGCPETGVGVVALGNGPWRPPSEEASAWAVVEHGL
                     ALLRAAALGQELPGDPPPPDPPPEVPSPATTARD"
     gene            1164..2027
                     /locus_tag="VFO47_03055"
     CDS             1164..2027
                     /locus_tag="VFO47_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787709.1"
                     /translation="MGNFPLPGMGRAVVAVPAPGAGPGWWAGASSAAPDGEGGFVVGY
                     RVRNGHDGIDQTVVARSSDGETFTTVATLDQARFGAQWMERPAVVRTLEGRWRLYVCC
                     GTPESKHWWIEALEAADPEGLAAAEARPVFPGDERTAVKDPIVQLRDGRWHAWICCHP
                     LDVPGAEDRMSSAYATSDDGWSWDWHGTVLSGRPDAWDARGARLTTVLSDGRAAYDGR
                     ASAEENWFERTGLAAATGRDGRFEQVEGPPVADVRYLDVLPLPGGGHRIWYEARLPDE
                     RHELRTEHVGG"
     gene            2030..>2462
                     /locus_tag="VFO47_03060"
     CDS             2030..>2462
                     /locus_tag="VFO47_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787710.1"
                     /translation="MAGVAGPAPQPWAEPGWAAQAGAWLAAEMERLGRPVTGPLREVR
                     SWELACVLRAPTTAGDVYLKANAPSPAFVDEGVVMATLARLFGDLVPAPLAVEAERGW
                     LVLDDFGEELGWEVPDEVVEEVARTFARLQVEATAHVGLLLG"
BASE COUNT          274 a          951 c          972 g          265 t
ORIGIN      
        1 gtcccgggcg gtcgtggcgg gcgaggggac ctcgggcggt gggtcgggag gcggtgggtc
       61 gccgggcagc tcctggccca gggccgccgc ccgcagcagg gccaggccgt gctcgaccac
      121 cgcccaggcg ctggcctcct ccgacggcgg ccgccagggc ccgttgccga gggcgaccac
      181 gccgaccccg gtctccgggc agccgaaggc gtagctgcag tagccggggc agtcgccctg
      241 gtggccgacc cggcggtagc cgtcctggtc gtccagctcg acgccgaggc cgtacccgta
      301 ccccccgtcg ggaatcggca gggcgggcgt cagcatggcc tcgcgggcgg ccgggcccag
      361 cagcggcgga tcgccggcga cgacggcgcg gaggaaccgg ccgaggtcgg gggcggtgca
      421 gagggtggcc cccgaccccg ccgccgtcgg cacccagggg gcggggacca tggccccgcc
      481 ggccagccgg acgtggccgg tggccgcccg ggcctgcgcc tcggcggtgg tgacggcctc
      541 cgagccggcc atgccgcagg ggtcgagcac gtgccgccgc accgcctcgg cataggagga
      601 gccggcgacc cgctccagca ccagcccgag cacggcgtag ccgacgttgg agtagcgggt
      661 cttctccccg ggcggccacc ccggcggggt gtgggcgagg gcgagcgcct ccaggtacgg
      721 cgacggcgag ccctccatgc cggtgacgat cccggcggtg tggcccagca ggtggcgcac
      781 gctgatcggc ccgaacgggg tcgccagggg cagccagggc aggtgctcgt ggaccggggc
      841 gtccaggtcg agccggcccg cctggtgctc gcgcagcagg gccgcggccg tcatcgcctt
      901 gccgaccgag gccaccggca tgagcgcctc ccgggccagc ggccggcccg aggcgacgtc
      961 ggtcacgccg acggccagct cgcgccgcca cccgcccggg ccgatcagcc cgaccgccag
     1021 cccgggcagg gcgacccgcc ggccggcgaa cgccgccagc cgctccgccg ccgcctccag
     1081 cgtcccctgt ccgcccaccc gcacccgctc ccctcgccgc cgcccgccgg accgtatgat
     1141 cgccgggatg ctagggtcgg cgcatgggaa atttccccct tcccgggatg ggccgggccg
     1201 tggtcgcggt gccggcgccc ggcgccgggc ccggctggtg ggccggcgcc tccagcgccg
     1261 cgccggacgg cgaggggggg ttcgtggtcg gctaccgggt gcgcaacggg cacgacggga
     1321 tcgaccagac ggtcgtggcc cgctcgtccg acggcgagac cttcaccacc gtggccacac
     1381 tcgaccaggc gcgcttcggc gcccagtgga tggagcgccc ggcggtggtc cgcacccttg
     1441 agggacgctg gcggctgtac gtctgctgcg gcacgcccga gagcaagcac tggtggatcg
     1501 aggccctcga ggccgccgac cccgagggcc tggccgcggc cgaggcccgc ccggtcttcc
     1561 ccggcgacga gcggacggcc gtcaaggacc cgatcgtgca gctgcgcgac gggcgctggc
     1621 acgcctggat ctgctgccac ccccttgacg tgcccggagc cgaggaccgc atgagctcgg
     1681 cctacgccac cagcgacgac ggctggtcgt gggactggca cggcaccgtc ctgagcggcc
     1741 ggccggatgc ctgggacgcc cgcggggccc ggctcaccac cgtcctcagc gacggccggg
     1801 ccgcctacga cggccgcgcc tcggccgagg agaactggtt cgagcggacc ggcctggccg
     1861 ccgcgaccgg ccgggacggc cgcttcgagc aggtcgaggg tccgcccgtg gccgacgtcc
     1921 gctacctgga cgtcctgccc ctgcccggcg gcggccaccg catctggtac gaggcccggc
     1981 tgcccgacga gcgccacgag ctgcgcaccg agcacgtcgg cggctgacgg tggccggggt
     2041 cgccgggccc gccccgcagc cgtgggccga gccggggtgg gccgcccagg ccggggcctg
     2101 gctggccgcc gagatggagc ggctgggccg gcccgtcacc gggccgctgc gggaggtccg
     2161 gtcctgggag ctggcctgcg tcctgcgggc gccgaccacc gccggggacg tctacctcaa
     2221 ggccaacgcg ccctcgcccg cgttcgtgga cgagggcgtg gtcatggcca cgctggcccg
     2281 gctgttcggc gacctcgtgc ccgccccgct ggccgtggag gccgagcgcg gctggctggt
     2341 gctggacgac ttcggcgagg agctcggctg ggaggtgccc gacgaggtcg tcgaggaggt
     2401 ggcccgcacc ttcgcccgcc tgcaggtcga ggccaccgcc cacgtcggcc tgctgctcgg
     2461 cg
//
LOCUS       DASSWR010000187         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_855698,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000187 DASSWR010000000
VERSION     DASSWR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855698"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..79
                     /locus_tag="VFO47_03065"
     CDS             <1..79
                     /locus_tag="VFO47_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET8787711.1"
                     /translation="QHLLEDPDVVIQGNRVLHLRYRVRR"
     gene            complement(225..1601)
                     /locus_tag="VFO47_03070"
     CDS             complement(225..1601)
                     /locus_tag="VFO47_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET8787712.1"
                     /translation="MPDATFGCPDLTTFCRLDELGLVVVGQRLEPDRAVLACRVLEAD
                     DGTGGWCRRCGCAGRPRDTVTRELAHEPLGWRPTTLLVTIRRYRCTGGGPLWRQDTSR
                     AAEPRARLSRRGLAWALEAIVCQHLSIARVAQGLGVAWNTANDAVLAEGRRVLINDPG
                     RLNGVKVIGVDEHVWRHTRRGDKYVTVIIDLTPVRAGTGPARLLDMIPGRSKAAFKAW
                     LTQRPQAWREAVEVVAMDGFTGFKTAAAEEIPAAVAVMDPFHVVRLAGDALNDCRRRV
                     QQTTRGRRGRKNDPLYRARRTLHTGADLLTDKQAARLTALFAIDEHVEVEATWGIYQR
                     MIAAYRHPDPAAGRELMVKLIESLSAGVPRPLVEIAKLGRTLSKRAADVLAYFDRPGT
                     SNGPTEAINGRLEHLRGSALGFRNLTNYIARSLLETGGFRPRLHPALVKSRSSWGSAK
                     DTTETGNQ"
     gene            complement(1700..2377)
                     /locus_tag="VFO47_03075"
     CDS             complement(1700..2377)
                     /locus_tag="VFO47_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8787713.1"
                     /translation="MAPATLRIRLLGELDLRLGEEPLPLLGSARAESLLAYLLLHRDT
                     PQPRQRLAFLLWPDSSESQARTNLRHLLHVLRRALPDADRFLEVTPRALRWREDAPWW
                     LDVAAFEEAVALAGRGPDGAGELPALREAAGLYRGDLLESGYDEWLLEPRERLRRDHL
                     RALERLVELLEAREEPAEAIGFAERLLRADPLREATYRSLMRLHDARGDRARALRAYH
                     GSSQLRV"
     gene            complement(2393..2518)
                     /locus_tag="VFO47_03080"
     CDS             complement(2393..2518)
                     /locus_tag="VFO47_03080"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787714.1"
                     /translation="MHTLGGLAIDATRADDVYDGGGWQVLRDPEGNEFCLLRPRS"
BASE COUNT          366 a          895 c          919 g          360 t
ORIGIN      
        1 gcagcacctg ttggaggatc ctgacgtggt gattcagggc aaccgggtgc ttcacctgcg
       61 ctatcgggtg cgccgttgac cgatctgagc gggtacgcga agaagtccac tgcgaacggt
      121 gacacaagat cggcctccgg ctgcgtttgc gcatcgctgt ccctggtcgg gcagccgacg
      181 ccgatccgcc ccagcacctc acctgcggca agttcaactc aagatcactg gttgccggtt
      241 tccgtcgtat ctttggccga cccccaggaa gagcggctct tcaccaatgc agggtgtagt
      301 cggggtctga atccgcctgt ctcgagtagg gatcgggcga tgtagttggt gaggttgcgg
      361 aagccgaggg cggagccgcg caggtgctcc aggcggccgt tgatcgcttc ggttggaccg
      421 ttgctggtgc cgggccggtc gaagtaggcc agtacgtcag cggctcgctt gctcagggtg
      481 cggccgagct tggcgatctc gaccagcggt cgggggacgc cggcgctgag cgactcgatc
      541 agcttgacca tcagctcgcg gcccgcggct gggtcggggt gccggtaggc ggcgatcatc
      601 cgctggtaga tgccccaggt ggcctcgacc tcgacgtgct catcgatggc gaacagggcc
      661 gtgagtcggg cggcctgctt gtcggtgagc aggtcggcac cggtgtgcag ggttcgccgg
      721 gcacggtaga gcgggtcgtt cttacggccg cggcggccgc gggtggtctg ctgaacgcgg
      781 cggcggcagt cgttgagggc gtcgccggcc aggcgcacca catggaaggg gtccatcacc
      841 gcgaccgcgg cggggatctc ctcagcggcg gcggtcttga agccggtgaa cccgtccatc
      901 gcgacgacct ccacggcctc gcgccacgcc tgggggcgct gggtaagcca ggccttgaac
      961 gcggccttgg agcggcccgg gatcatgtcc agcagcctcg ctggacccgt accggcgcgc
     1021 accggcgtga ggtcgatgat cacggtgacg tacttgtccc cgcgacgggt gtggcgccac
     1081 acgtgctcat cgaccccgat gaccttcaca ccgttcaacc ggccgggatc gttgatcagc
     1141 acccgtcggc cctcggccag gaccgcgtcg ttggcggtgt tccacgcgac gccaagccct
     1201 tgggcgacgc gagcgatgct caagtgctga cacacgatcg cctccagcgc ccaggccagt
     1261 ccacgacgcg acagcctggc ccgcggctcg gccgcgcggc tggtgtcctg gcgccacaga
     1321 ggcccccccc cggtgcaccg gtaccggcgg atcgtcacga gcagggtggt cggtcgccag
     1381 cccagcggct cgtgcgccag ctcccgcgtg acggtgtccc ggggtcttcc tgcacacccg
     1441 cagcgtcggc accagccccc ggtgccgtca tcggcctcca ggacccggca cgccaggacc
     1501 gcgcgatcgg gctccagacg ctggccgacc acgaccaggc cgagctcatc caggcggcag
     1561 aacgtggtca ggtcagggca gccgaaggta gcgtcaggca cgtcgaggtc ttccagatgg
     1621 gcggcctagg aacctccatc ctcggaagac ctcgacacct accccgggac cgacgcgcca
     1681 gccaccatcc aaccagcgac tacaccctca actgtgaaga gccgtgatag gcccggagcg
     1741 ccctggcccg gtcgccgcgg gcgtcgtgga gccgcatcag ggaccggtag gtggcctcgc
     1801 gcagcgggtc ggcccgcagc agccgctccg cgaacccgat cgcctcggcc ggctcctccc
     1861 gcgcctccag cagctcgacc agccgctcca gggcccggag gtggtcccgg cgcagccgct
     1921 cgcgcggctc cagcagccac tcgtcgtagc cgctctcgag caggtcgccc cggtacagcc
     1981 cggccgcctc ccgcagggcc ggcagctcac ccgcgccgtc cggcccccgc ccggccaggg
     2041 cgaccgcctc ctcgaaggcg gcaacgtcca accaccaggg ggcgtcctcc cgccagcgca
     2101 acgcccgcgg ggtcacctcg aggaaccggt cggcgtcggg cagggcgcgc cgcaggacgt
     2161 gcagcaggtg gcgcaggttg gtccgggcct gggactcgct cgagtcgggc cacaggagga
     2221 aggccaggcg ctggcggggc tggggcgtat cgcggtggag cagcaggtag gcgagcagcg
     2281 actcggcccg ggccgagccg agcagcggca gcggctcctc gccgaggcgc aggtcgagct
     2341 cgccgaggag ccggatgcgc agcgtggccg gtgccatggg accgcccgga gctcaggatc
     2401 gggggcggag caggcagaac tcgttgccct cggggtcgcg caggacctgc cagccgccgc
     2461 cgtcgtagac gtcgtcggcc cgggtcgcgt cgatggccag cccgcccagg gtgtgcaggg
     2521 tggcgccgtc gtcgtcgacc
//
LOCUS       DASSWR010000188         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_149400,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000188 DASSWR010000000
VERSION     DASSWR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149400"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFO47_03085"
     CDS             complement(<1..1283)
                     /locus_tag="VFO47_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787715.1"
                     /translation="METASTSRRALVAGSIGNVVEWYDFAVYGAFATVVAATYFPGGD
                     PVAGLAASFAVFATAFLARPVGAVVFGRLGDRVGRRQVLVTVILLMAAATAGIGLLPG
                     RATIGVLAPLLLIVFRMLQGLSVGGEAAGATAFVVEYAREGRRGWYGAWLWSTLSLGV
                     AGGIGAALVLTWALPPAVLQDWGWRLAFLAAVPLGLVGLYLRLALDDTPRFRSLQRAG
                     AVARWPVGDTLAGHRSRLLLGLVLVAAASLAFNLFFIYLPGYLAAAVGIPLARTLPAA
                     LTGLLLVAAAGLAAGRLSDRVGRRPFLLAGTAGLLLVTLPAFLLIRGAGPPGRALGFV
                     LVALSLSCLAVVPSHLAELFPTAVRSTALAISYGLGTALFGGTAPFLATWLVRRTGSP
                     VAPAVYATLLAAAAVVAAVASRETAFQSLPAADRAG"
     gene            complement(1399..1539)
                     /locus_tag="VFO47_03090"
                     /pseudo
     CDS             complement(1399..1539)
                     /locus_tag="VFO47_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
     gene            complement(1605..>2083)
                     /locus_tag="VFO47_03095"
     CDS             complement(1605..>2083)
                     /locus_tag="VFO47_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET8787716.1"
                     /translation="RWSGVPFFIRTGKCLPVTRTELRLVFKHPPRLGFFSRSRRPEPD
                     QLVVKLYPSTGVRLKVDAQRGDRPDPEQIHLDMEFADEGGEAATPYEVLLHAAMVGQS
                     TRFTRQDGVEEAWRVLQPLLDSPPPVHPYAKGSWGPDEANRLLAGHGRWYGPWETE"
BASE COUNT          334 a          784 c          773 g          192 t
ORIGIN      
        1 ccggccctgt cggcggcggg cagcgactgg aaggcggtct cccgcgacgc cacggccgcg
       61 accaccgccg cggcggccag cagggtcgcg tacacggccg gggcgaccgg gctgccggtc
      121 cgccgcacca gccaggtggc caggaacggc gccgtgcccc cgaacagggc cgtgcccagc
      181 ccgtagctga tggccagggc ggtggagcgg accgcggtgg ggaacagctc ggccaggtgg
      241 gacggcacca cggccaggca gctcagggac agcgcgacca ggacgaagcc gagggcccgc
      301 cccggcggcc cggccccgcg gatcagcagg aaggcgggca gggtgaccag cagcaggccg
      361 gccgtcccgg cgagcaggaa cggccgccgg cccacccggt ccgagagccg cccggcggcc
      421 agcccggccg ccgccaccag cagcaggccc gtcagcgcgg cgggcagcgt cctggccagg
      481 gggatgccga ccgcggcggc caggtagccg ggcaggtaga tgaagaacag gttgaaggcg
      541 agcgacgccg cggccaccag gaccaggccg agcagcagcc ggctccggtg accggccagg
      601 gtgtcgccga ccggccaccg ggcgacggcg ccggcccgct gcaggctccg gaaccgcggg
      661 gtgtcgtcca gggccaggcg caggtagagc ccgaccagcc cgagcgggac cgcggccagg
      721 aaggccagcc gccagcccca gtcctggagc acggccggcg ggagcgccca ggtcagcacc
      781 agggcggcgc cgatcccgcc ggccaccccg aggctcaggg tcgaccacag ccaggctccg
      841 taccagccgc gccggccctc gcgcgcgtac tcgaccacga aggcggtggc cccggcggcc
      901 tcgccgccga ccgagagccc ctggagcatc cggaacacga tcaggagcaa tggcgccagc
      961 acgccgatgg tggcgcgccc gggcagcagc ccgatgccgg ccgtggccgc cgccatcagc
     1021 aggatcaccg tgaccagcac ctggcggcgg ccgacccggt cgccgagccg gccgaacacg
     1081 accgcgccga cgggccgggc caggaacgcg gtggcgaaca cggcgaagct cgccgccagc
     1141 ccggcgaccg ggtcgccgcc cgggaagtag gtggcggcga cgaccgtggc gaacgccccg
     1201 tagacggcga agtcgtacca ctcgacgacg ttgccgatgc tgcccgccac cagcgcccgc
     1261 ctggacgtcg aggccgtctc catcagccgc ccccgttcat cccaggacct cttccaggcg
     1321 tccggcgaac agccctcggg gggaccggtc ctgctcgccg accacggcca gggccgagac
     1381 cagccagaac gagcagatca gcaacgaccc gaacacactg ggcgaatcaa accggggcac
     1441 acacaaccag tccaccgccc catcagcggc caccaacgcc ccggtatgac aattcgacaa
     1501 gaacgcatac ccgtcgatcg gggagaacgg cgacggcatc gccgcactct gaaacccctc
     1561 ggtgccggcg gcgacggtgg cggcgcgctt ggcgcccttg gggctcattc ggtctcccac
     1621 gggccgtacc agcggccgtg cccggccagc aggcggttgg cttcgtcggg accccagctc
     1681 cccttggcgt agggatggac cgggggtggg ctgtcgagca gcggctgcag gacccgccag
     1741 gcttcctcca cgccgtcctg gcgggtgaag cgggtcgact ggcccaccat ggccgcgtgc
     1801 agcagcacct catagggggt ggccgcctcg ccgccctcgt cggcgaactc catgtcgagg
     1861 tggatctgct cggggtccgg ccggtcgccg cgctgggcgt cgaccttcag ccggacgccg
     1921 gtggaggggt agagcttgac cacgagctgg tcgggctcgg gtcgccggct cctgctgaag
     1981 aagcccagcc ggggcgggtg cttgaagacc agccgcagct cggtccgggt gaccggcagg
     2041 cacttgccgg tgcggatgaa gaacggcacc ccggaccagc gcc
//
LOCUS       DASSWR010000189         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_741645,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000189 DASSWR010000000
VERSION     DASSWR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741645"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFO47_03100"
     CDS             <1..912
                     /locus_tag="VFO47_03100"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET8787717.1"
                     /translation="RQLALHDRFVAAGVTAVVGMGGSPGITNVLAALAAHGLDTVSEV
                     EVRLGVADFAPSSAPVPVPYAIQTILDEFAVPAVTFRDGRLVEVPAMSEQEELDFPPP
                     VGRVRVGHTLHSEIATLPLHFADRGIRSVSFKVGFPADFMDKLTLLTALGLAGTSPVE
                     LPSGTVVPRELLVHAITTTAITPGPEAAPDDAEAIWVRVRGRRAGPSGDPALAPREAS
                     PDLLGAPVERLAELVVRPHPAWQAGSGQLDTGVPPSIVAQLLAAKVIDRPGVLAPEDA
                     VPPEPFLAELATRNMEVTLITRERSHR"
     gene            909..2255
                     /gene="gabT"
                     /locus_tag="VFO47_03105"
     CDS             909..2255
                     /gene="gabT"
                     /locus_tag="VFO47_03105"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET8787718.1"
                     /translation="MSMPQERKLAGAVPGPGSQALEERRRRAVARGVATMTPVYAAAA
                     HGAVIEDVDGNRFIDFTGGLGVLNAGHTPPSVTQAVKDQVERYLHTCQHAVMNEPYVA
                     VAEALNRITPGDHDKRTLLVNSGAEATENVVKIARAATGRPGVVVFDNAFHGRTLLAL
                     AMTGKVTPYKQGYQPFPSEIYRMPYPYCYRCPFHLDHSTCGLACADYIEEEIKVHVGP
                     QNVACLIVEPVQGEGGFIAPPPGWLERIADMCRRLEIPFVADEVQSGFGRTGTLYATE
                     QHPGVEPDFTLSAKSIAAGLPLAAVTGRAELMDAPGPGGLGGTYGGNPLACVAALATI
                     ELFEQGDLLERARRMGETLDGRLRELGERHRIVGEVRGLGAMMAMELVTDRQTKAPAR
                     QETSQVVAEAARNGLLTLKAGIHDNVVRILAPLVMEERLLAEGLDILDQALATASA"
BASE COUNT          325 a          901 c          838 g          302 t
ORIGIN      
        1 cggcagctgg ccctgcacga ccggttcgtg gccgccgggg tcaccgcggt ggtcgggatg
       61 gggggcagcc cgggcatcac caacgtgctg gccgccctgg ccgcccacgg gctggacacc
      121 gtctccgagg tcgaggtccg cctgggcgtg gccgacttcg ccccctccag cgcccccgtg
      181 cccgtgccgt acgccatcca gacgatcctc gacgagttcg ccgtcccggc ggtcaccttc
      241 cgcgacggcc ggctggtcga ggtgccggcc atgagcgagc aggaggagct cgacttcccg
      301 ccgccggtgg ggcgggtccg ggtcgggcac acgctgcact cggagatcgc gaccctgccg
      361 ctgcacttcg ccgaccgcgg gatccggtcg gtgtcgttca aggtcggctt cccggccgac
      421 ttcatggata agctgaccct cctgacggcg ctcggtcttg ccggtacctc tccggtcgag
      481 ctgccgagcg gcacggtggt cccgcgcgag ctgctcgtcc acgccatcac cacgaccgcc
      541 atcacgcccg gccccgaggc cgccccggac gacgccgagg cgatctgggt ccgggtgcga
      601 gggcgacggg ccggaccgtc gggggatccg gccctcgccc ctcgcgaagc atcccctgac
      661 ctcctgggcg ctccggtcga gcggctggcc gagctcgtgg tccggcccca cccggcctgg
      721 caggccggca gcggccagct cgacaccggc gtgccgccca gcatcgtcgc ccagctcctg
      781 gcggccaagg tgatcgaccg gccgggcgtg ctcgcccccg aggacgccgt cccgcccgag
      841 cccttcctgg ccgagctggc aacccgcaac atggaggtca cgctgatcac ccgcgagagg
      901 agccaccgat gagcatgccg caggagcgga aactggccgg tgccgtcccg gggccgggca
      961 gccaggccct ggaggagcgg cggcgcaggg cggtggcccg gggcgtggcc accatgacgc
     1021 cggtgtacgc ggcggccgcc cacggcgcgg tcatcgagga cgtcgacggc aaccgcttca
     1081 tcgacttcac cggcggcctg ggggtgctga acgccggcca caccccgccg tcggtcaccc
     1141 aggcggtcaa ggaccaggtc gagcgctacc tccacacctg ccagcacgcg gtgatgaacg
     1201 agccgtacgt ggccgtcgcc gaggccctca accggatcac ccccggcgac cacgacaagc
     1261 ggaccctgct ggtgaactcc ggggccgagg cgaccgagaa cgtggtcaag atcgcccggg
     1321 ccgccaccgg ccgcccaggg gtggtcgtgt tcgacaacgc cttccacggc cgcaccctgc
     1381 tggccctggc catgaccggc aaggtcaccc cctacaagca gggctaccag ccgttcccga
     1441 gcgagatcta ccggatgccc tacccctact gctaccgctg ccccttccac ctcgaccact
     1501 ccacctgtgg tctggcctgc gccgactaca tcgaggagga gatcaaggtc cacgtcgggc
     1561 cccagaacgt ggcctgcctg atcgtggagc cggtccaggg cgagggcggc ttcatcgccc
     1621 cgccgcccgg ctggctggag cggatcgccg acatgtgccg gcgcctggag atcccgttcg
     1681 tggccgacga ggtccagtcg ggcttcggcc gcaccggcac cctgtacgcc accgagcagc
     1741 acccgggcgt cgagcccgac ttcaccctgt cggccaagtc gatcgcggcc gggctgcccc
     1801 tggccgcggt caccgggcgg gccgagctga tggacgcccc cggccccggc gggctcggcg
     1861 gcacctacgg tggcaacccg ctggcctgcg tggccgcgct ggccaccatc gagctgttcg
     1921 agcagggcga cctgctggag cgggcccggc ggatgggcga gacccttgac gggcgcctac
     1981 gcgagctggg cgagcgccac cggatcgtgg gcgaggtccg ggggctgggc gcgatgatgg
     2041 ccatggagct ggtcaccgac cggcagacca aggcgccggc caggcaggag accagccagg
     2101 tggtggccga ggcggcccgc aacggcctgc tcaccctcaa ggccggcatc cacgacaacg
     2161 tcgtgcgcat cctcgccccc ctggtcatgg aggagcggct gctggccgag gggctcgaca
     2221 tcctcgacca ggccctggcc accgcctcgg cctgaaggtg ccgccgggcg ggtaccctcc
     2281 acgtccatga ggacatccat gtgggtcgcc ggggcggatc tgcccggccg gggcgcggtc
     2341 gaggtggtcg ccaacccggc caccga
//
LOCUS       DASSWR010000190         5575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_911508,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000190 DASSWR010000000
VERSION     DASSWR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911508"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFO47_03110"
     CDS             <1..424
                     /locus_tag="VFO47_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787719.1"
                     /translation="GGLVLFAVRRTVARQEAGVPPPAACPPAGTPLLAEQARYLGTTY
                     APSSVRRFNGHGLLGRRLVGLALDEGGLRVDDRWCIPPADLRGVEITSHHAGKEVYAD
                     KVLVVDWRLGPATLRSGFALDEDTAPAWAARLRPLVPR"
     gene            421..1521
                     /gene="carA"
                     /locus_tag="VFO47_03115"
     CDS             421..1521
                     /gene="carA"
                     /locus_tag="VFO47_03115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET8787720.1"
                     /translation="MSPREALLVLEDGTAFRGEAFTAQPGTVAGEVVFNTAMGGYQEV
                     LTDPSYAGQLVCMAAPEVGNYGTAPADAESERVQVAAFLVRQAARRPSSWRAERSLAE
                     ELEAAGVPGVEGLDTRRLVRHLRQRGAMRGALSTETTDPAALRATALAAPDPNGLAMV
                     DRVTRSEPVELPADGDERLRVVLYDFGAKANIARRLRRLGARVLAVPASTPAAEALAW
                     RPDGVMLSNGPGDPATVGHGVRATRELLGKVPVFGICLGHQLLGQAVGGRTVRLKFGH
                     RGVNQPVVELATGRVAVTSHNHGFAVDGDSLPAGVRVTHANANDGVVEGLECLDVPAF
                     SVQYHPEAAPGPHDAHPLFERFADLMEGARHG"
     gene            1514..1924
                     /locus_tag="VFO47_03120"
     CDS             1514..1924
                     /locus_tag="VFO47_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787721.1"
                     /translation="MAEQRTFRALGGVDQGALPPAGEVADALRAEFERVFTLKAGLRG
                     EAEAGVSPTGRRTTAQDEAKLRTLVASVEGASRLAIRLRLLSPAQVRSLWAEAMARGL
                     YDGWDAGQSAYDQAAEGAPPSTSGIPPEVLRGDA"
     gene            1924..5265
                     /gene="carB"
                     /locus_tag="VFO47_03125"
     CDS             1924..5265
                     /gene="carB"
                     /locus_tag="VFO47_03125"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET8787722.1"
                     /translation="MPLREDLGCILVVGSGPIVIGQAAEFDYSGTQACRALRGLGLRV
                     VLVNSNPATIMTDPEVADATYLEPLTPGPVAAVLEAERPDALLPTLGGQTALNLAVAL
                     HENGTLERLGVELIGATVDAVRRAESRVEFTETCRAAGLDLPRGVEVGSVADGLAAAG
                     NLGLPVVIRPSFTLGGWGGGIARTAGELERLLAEGLTASPVGRVLIEESVLGWKEFEL
                     EVMRDRADNAVVVCSIENVDPMGVHTGDSVTVAPQMTLSDREYQEMRDDALTVLRAVG
                     VETGGANVQFAVDPRTGRRVVIEMNPRVSRSSALASKATGFPIAKVAAQLAVGFTLDE
                     IRNEVTGKTAAAFEPTLDYVVVKVPRFNFEKFPGADPELTTRMKSVGEVMAIGRTFPE
                     ALQKACRSLETGRDGLGDPAGDPAPGSPQAAPEALLAACGLPRADRLEAVERALRLGR
                     GVEEVAAASDVDPWFVDGIARIVEVGAEVAAAASIDDLGRGLLWRAKRHGFSDPQLAA
                     AVRATEGEVRTRRRALGVEPVYRSVDTCAGEFEASTPYHYSTYEEETEVPATGRRRVV
                     VLGSGPNRIGQGVEFDTACVHAVQALREAGYETVMVNCNPETVSTDYDIADRLYVEPL
                     TCEDVLEVCRAEAAGGELVGVIVTMGGQTPLQLAQGLAAAGVPLLGTPPDAIDLAEDR
                     GRFGTVLEQCGLDAPPWGQAATVEQALAVADRVGYPVLVRPSYVLGGRSMEVCYDADG
                     LRRSSALAGVGRGGSNGGPGVGSPGLPGERPVLVDRFLEDAVEVDVDALCDATGQVLV
                     CGVMEHIEEAGIHSGDSACVLPTITLGADQVAAVEEATARLGRALGVVGILNVQFAFK
                     GQRLYVLEANPRASRSVPFVEKVTGLPVAKAAAWLMTGSTLAGLDLRASEPAHVGVKE
                     AVLPFARFPGADSLLGPEMRSTGEVLGIDARFGAAFAKSQEAAYGALPVKGVAFLSVR
                     NADKRAIVLPAKRLADLGFTLVATTGTAGVLRRNGMEAAVVRKGHEGPDNVVDKIRAG
                     EIDLVVNTPAGPGAREDGYDIRIAAVAADIPCITTLSGLTAVVQGIEARLAGELAVRP
                     LQAWHDGPAGNGPAPGRPPGPGERP"
     gene            5262..>5575
                     /locus_tag="VFO47_03130"
     CDS             5262..>5575
                     /locus_tag="VFO47_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET8787723.1"
                     /translation="MTPVRERCEVLERRRTGAYQALTLVAPRTAEHARPGQFVHLLAG
                     DDRSFPLRRPFSVHKVERPGTGMGTVEVVFDVVGAGTRALARLRPHDVVDALGPLGRP
                     FDP"
BASE COUNT          650 a         2165 c         2075 g          685 t
ORIGIN      
        1 cggtgggctg gtcctgttcg cggtgcggcg gacggtggcc cgccaggagg ccggcgtgcc
       61 cccaccggcc gcctgcccgc cggcggggac gcccctgctg gccgagcagg cccgctacct
      121 gggcaccacc tacgccccct ccagcgtccg ccgcttcaac ggccacggcc tgctcggccg
      181 ccgcctggtc ggcctcgccc tcgacgaggg cggcctgcgg gtcgacgacc gttggtgcat
      241 ccccccggcc gacctgcgcg gggtcgagat cacctcccac cacgccggca aggaggtcta
      301 cgccgacaag gtcctggtcg tcgactggcg gctcgggccg gccaccctcc gctccgggtt
      361 cgccctcgac gaggacaccg cccccgcctg ggcggcgcgg ctgcggccgc tggtgccccg
      421 atgagccccc gcgaggcgct gctggtgctg gaggacggga ccgccttccg gggcgaggcg
      481 ttcaccgccc agccgggcac ggtggccggg gaggtcgtgt tcaacaccgc catgggcggc
      541 taccaggagg tcctgaccga cccctcctac gccgggcagc tggtctgcat ggccgcgccc
      601 gaggtcggca actacggcac cgccccggcc gacgccgagt ccgagcgggt ccaggtggcc
      661 gcgttcctgg tccggcaggc ggcccggcgg ccctcgagct ggcgggccga gcgcagcctg
      721 gccgaggagc tggaggcggc cggcgtgccc ggggtcgagg gcctcgacac ccgccggctg
      781 gtccggcacc tgcgccagcg cggggccatg cgcggcgccc tctccaccga gaccaccgac
      841 ccggcggccc tgcgcgcgac cgccctggcc gcgcccgacc ccaacggcct ggccatggtc
      901 gaccgggtca cccgctccga gccggtcgag ctgcccgccg acggcgacga gcgcctgcgg
      961 gtggtgctct acgacttcgg ggccaaggcc aacatcgccc ggcggctgcg ccggctcggg
     1021 gcccgggtgc tggccgtgcc ggcgtccacc ccggccgccg aggcgctggc ctggcggccc
     1081 gacggggtga tgctgtccaa cggccccggc gacccggcca ccgtcggcca cggcgtccgg
     1141 gcgacccggg agctgctcgg caaggtgccg gtgttcggca tctgcctcgg ccaccagctg
     1201 ctcggccagg ccgtcggcgg ccgcacggtg cggctcaagt tcggccaccg cggcgtcaac
     1261 cagccggtgg tcgagctggc caccgggcgg gtcgcggtca ccagccacaa ccacggtttc
     1321 gcggtcgacg gcgacagctt gccggccggg gtgcgggtca cccatgccaa cgccaacgac
     1381 ggcgtcgtcg agggcctgga gtgcctggac gtgcccgcct tctcggtcca gtaccacccc
     1441 gaggccgccc ccggtcccca cgacgcccac cccctgttcg agcggttcgc cgacctgatg
     1501 gagggagccc gacatggctg agcagcggac cttccgggcc cttggcgggg tcgaccaggg
     1561 cgccctgccc ccggcggggg aggtcgccga cgccctccgg gccgagttcg agcgggtctt
     1621 caccctcaag gccggcctgc gcggcgaggc cgaggccggc gtctcgccca ccggccggcg
     1681 caccaccgcc caggacgagg ccaagctgcg caccctggtg gccagcgtcg agggggccag
     1741 ccgcctggcc atccgcctgc gcctgctcag cccggcccag gtccggtccc tgtgggccga
     1801 ggccatggcc cggggcctgt acgacggctg ggacgccggc cagagcgcct acgaccaggc
     1861 ggcggagggc gcgcccccga gcaccagcgg catcccgccc gaggtcctgc ggggtgacgc
     1921 ctagtgcccc tccgggagga cctgggctgc atcctggtcg tggggtcggg gccgatcgtc
     1981 atcgggcagg cggccgagtt cgactactcc ggcacccagg cctgccgggc cctgcgcggg
     2041 ctggggctgc gggtggtcct ggtcaactcc aacccggcca cgatcatgac cgaccccgag
     2101 gtggccgacg ccacctacct ggagccgctc acccccggcc cggtggcggc cgtgctcgag
     2161 gccgagcggc ccgacgccct gctgcccacc ctgggcgggc agacggccct caacctggcc
     2221 gtggccctgc acgagaacgg caccctggag cggctcgggg tcgagctgat cggggccacc
     2281 gtggacgcgg tccgccgggc cgagtcccgg gtcgagttca ccgagacctg ccgggccgcc
     2341 gggctcgacc tgccccgcgg ggtcgaggtc gggtcggtcg ccgacggcct ggccgccgcc
     2401 ggaaatctgg ggctgccggt ggtcatccgg ccctcgttca ccctcggcgg ctggggcggc
     2461 ggcatcgccc ggaccgccgg tgagctggag cggctgctgg ccgagggcct gaccgccagc
     2521 ccggtcggcc gggtgctgat cgaggagtcg gtgctcggct ggaaggagtt cgagctcgag
     2581 gtgatgcgcg accgggccga caacgccgtg gtcgtctgct cgatcgagaa cgtcgacccg
     2641 atgggcgtcc acaccggcga ctcggtcacc gtcgcccccc agatgaccct gtcggaccgc
     2701 gagtaccagg agatgcgcga cgacgccctg accgtgctgc gggccgtcgg ggtcgagacc
     2761 ggcggggcca acgtccagtt cgccgtcgac ccccggaccg gccgccgggt cgtgatcgag
     2821 atgaacccgc gggtgtcgcg gtcctcggcg ctggcctcca aggccaccgg gttcccgatc
     2881 gccaaggtgg ccgcccagct ggccgtcggc ttcaccctcg acgagatccg caacgaggtc
     2941 accggcaaga ccgccgccgc cttcgagccg accctcgact acgtggtcgt caaggtcccc
     3001 cggttcaact tcgagaagtt ccccggcgcc gacccggagc tgaccacccg catgaagtcg
     3061 gtgggggagg tcatggccat cggccgcacc ttccccgagg ccctccagaa ggcctgccgg
     3121 tcgctggaga cgggccgcga cggcctcggc gacccggccg gcgacccggc gccgggcagc
     3181 ccccaggccg cccccgaagc cctgctggcc gcctgcggcc tgccccgcgc cgaccggctg
     3241 gaggcggtcg agcgggccct gcggctgggc cgcggcgtcg aggaggtggc cgccgcctcc
     3301 gacgttgatc cctggttcgt ggacggcatc gcccggatcg tcgaggtcgg ggccgaggtc
     3361 gccgccgccg cctccatcga cgacctcggc cggggcctgc tctggcgggc caagcgccac
     3421 ggcttctccg acccccagct ggccgccgcc gtcagggcca ccgagggcga ggtccggacc
     3481 cggcgccggg cgctgggggt cgagcccgtg taccgctcgg tcgacacctg cgccggcgag
     3541 ttcgaggcct ccacccccta ccactactcg acctacgagg aggagaccga ggtcccggcc
     3601 accggccgcc gccgggtggt ggtgctcggg tcggggccca accggatcgg ccagggggtc
     3661 gagttcgaca cggcctgcgt ccacgccgtc caggcgctgc gcgaggccgg ctacgagacg
     3721 gtgatggtca actgcaaccc cgagaccgtc tcgaccgact acgacatcgc cgaccggctc
     3781 tatgtcgagc cgctgacctg cgaggacgtc ctcgaggtgt gccgcgccga ggcggcaggg
     3841 ggcgagctcg tcggggtgat cgtgaccatg ggcggccaga ccccgctgca gctcgcccag
     3901 gggctggccg cggccggggt gccgctgctc ggcaccccgc ccgacgccat cgacctggcc
     3961 gaggaccggg gccgcttcgg gacggtcctg gagcagtgcg gcctggacgc cccgccctgg
     4021 ggccaggcgg ccaccgtcga gcaggccctg gccgtggccg accgggtcgg ctacccggtc
     4081 ctggtccgcc cttcgtacgt gctcggcggc cgctccatgg aggtctgcta cgacgccgac
     4141 gggctgcgcc gctccagcgc cctggccggc gtcggccggg gcgggtccaa cggcgggccg
     4201 ggtgtgggct ccccaggtct ccccggcgag cggcccgtgc tggtcgaccg gttcctggag
     4261 gacgcggtcg aggtcgacgt cgacgccctc tgcgacgcca ccgggcaggt gctggtctgc
     4321 ggggtgatgg agcacatcga ggaggccggc atccactccg gcgactcggc ctgcgtgctc
     4381 ccgacgatca ccctcggcgc cgaccaggtc gcggccgtcg aggaggccac cgcccgcctc
     4441 ggccgggccc tcggggtcgt cgggatcctc aacgtccagt tcgccttcaa gggccagcgg
     4501 ctgtacgtgc tcgaggccaa cccgagggcg agcaggtcgg tcccgttcgt ggagaaggtc
     4561 accggcctgc ccgtggccaa ggcggcggcc tggctgatga ccggctcgac cctggccggg
     4621 ctcgacctgc gggcgtccga gccggcccac gtcggggtca aggaggcggt gctgccgttc
     4681 gcccgcttcc ccggggccga ctcgctgctc ggcccggaga tgcgctccac cggcgaggtg
     4741 ctggggatcg acgcccgctt cggggccgcc ttcgccaaga gccaggaggc cgcctacggg
     4801 gccctgccgg tcaagggggt ggccttcctg tcggtccgca acgccgacaa gcgggccatc
     4861 gtgctgccgg ccaagcgcct cgccgacctc ggcttcaccc tggtcgcgac caccggcacg
     4921 gccggggtcc tgcgccgcaa cggcatggag gcggcggtcg tccgcaaggg ccacgagggg
     4981 cccgacaacg tggtcgacaa gatccgcgcc ggagagatcg acctggtcgt caacacgccc
     5041 gccggccccg gggcccgcga ggacggctac gacatccgca tcgccgccgt ggccgccgac
     5101 atcccctgca tcaccaccct gtccggcctc accgccgtcg tccagggcat cgaggcccgc
     5161 ctggccggcg agctggccgt ccgtcccctc caggcctggc acgacggccc agccggcaac
     5221 gggccggcgc ctggccggcc gccggggccc ggggaacggc catgaccccg gtccgggagc
     5281 ggtgcgaggt cctggagcgg cgccggaccg gggcctacca ggcgctcacc ctggtcgcgc
     5341 cccggaccgc cgagcacgcc cggccggggc agttcgtcca cctgctggcc ggcgacgacc
     5401 gctcgttccc gctgcggcgg cccttctcgg tccacaaggt cgagcggccg ggcacgggga
     5461 tgggaacggt cgaggtcgtg ttcgacgtcg tcggggccgg gacccgggcc ctggcccggc
     5521 tgcggcccca cgacgtggtc gacgccctcg ggcccctggg gcggccgttc gaccc
//
LOCUS       DASSWR010000191         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_375311,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000191 DASSWR010000000
VERSION     DASSWR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375311"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFO47_03135"
     CDS             <1..703
                     /locus_tag="VFO47_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039638715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8787724.1"
                     /translation="ARQSLGQVQLVARNARRTLARRPEDGRLGRLVGELEETITATER
                     LLAQTDQRLAGNRVIPDRLVSLADPDARPIRKGKPGRPTEFGYTLLLAEEERGFVCDH
                     QLQRGNPPDAPQLVPAVQRVSGVTGRPPGTVVGDRGFGTAANDQAMEALGVKRVGLQR
                     TGTPGKARLGLERTRRFRRLRNWRVGIEARISHLKGGFGLRRTRLRRLPGARTWVGLG
                     IFAYNRQRMTVVAAG"
     gene            1685..2509
                     /locus_tag="VFO47_03140"
     CDS             1685..2509
                     /locus_tag="VFO47_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787725.1"
                     /translation="MPRKPAARKGREAASKAHTAKPNLFDDIARQVNADQEAKDRAAG
                     RDDLTPAVAKSVDQATVKPSGINRAATRPGTPARPVKPPRLTKIQANGNGVSFSGRPT
                     TKVETVAARVKKAGLRVRGDADVDAVAARLRATPGAVSAWLATRSGAPTKPQAKVGTK
                     QPATAKPRQMKPDKAPTITLGHLWGDRQPDWLYRCTLVTRYEVRPDGTVWSTLDQVLG
                     PDGKVVEDFWRLPLDFRPTQQIQGNGRKLVLKDRTIAGLQTWLIRAGLTTPRDITT"
BASE COUNT          511 a         1017 c          997 g          383 t
ORIGIN      
        1 cgccaggcag tcgcttggcc aggtccagct ggtggcgcgc aacgcccgcc ggacgctggc
       61 ccgtcgcccc gaggatgggc gactgggtcg gctggtcggt gagctggagg agacgatcac
      121 cgcgaccgag cggctgctgg cccagaccga ccagcggctg gccggcaacc gggtgattcc
      181 cgaccggttg gtgtcgctgg ccgacccgga cgcccgcccg atccgcaagg gcaagccggg
      241 ccggcccacc gagttcggct acacgctgct gctggccgag gaggaacgcg ggttcgtctg
      301 tgaccaccag ctccagcggg gcaacccgcc cgacgctccc cagctcgtac ccgccgtcca
      361 gcgggtcagc ggcgtcaccg gccggccacc gggcacggtg gtcggcgacc gcggcttcgg
      421 cactgccgcc aacgaccaag caatggaggc gttgggcgtg aaacgggtcg ggctgcagcg
      481 caccggcacg ccgggtaagg cgcggctggg gttggagcgg acccggcggt tccggcggct
      541 gcgcaactgg cgggtcggga tcgaggcccg catcagccac ctcaagggtg gctttgggtt
      601 gcggcgcacc cggcttcggc ggttgccggg tgcgcggacc tgggtcggac tggggatctt
      661 cgcctacaac cggcagcgca tgaccgtggt cgccgccggg tgatcaagca gtggaccggc
      721 aaccagccag gcgttccatc catccggcat gaaacgccca gctgctcacc tgcccaattt
      781 caggggcaag tagctagacg gggggccaag cagagagcac tcggggccac cgtggaaggg
      841 cccgatccca cggggactcg gcaggatcag cgcggcggcc ccgagtgccg tcctgttaac
      901 cctcggggag ggcggggaat cctcggagcc aagcccctcc agcgccgcag cagccggtca
      961 tctctcccgc caagaagatc cgtcccgacc gctgcggcac tgccacccta agcctgagcc
     1021 tgtagctgca aacgcttcaa tcgcgtcgaa tcagcggaag taccgattcg ctggccgatg
     1081 caactcggac agcagctatt actggtggcc gccgacaagc gaagagcggg cggctcccgc
     1141 cgggggggcc aagcaggaga ccgcccgcac tactggaccg gttaagtaag tctagcgctc
     1201 ggaggccagc tgtcgttaca tcgggttgct cgccgctcgc gcttggcgag gcgctcctcg
     1261 gcctccagca gccgacgaac caccagcagc atgcgcatca ggtcatcgag cgcctggctg
     1321 gcggcttgcc agtccggatt gctgaccgct cgggtaagga gcgcgtgggc ttgctcgcgc
     1381 tcctcagggt catccgccgc gggtcctcca tccccctcgc cccctcgatc cactcaggtc
     1441 ctttccgacg ccgacgtgga ctgctcgcca ccaccgcttg gaccaggctc cacccggccg
     1501 cctgaccgtt gtaagcgccg cgggaaggac agtgaaaggg caacgtggac acgcaggtgg
     1561 ggttgttggc ggcccgtggt ctcctacagc gatggctgta cgacagcctg ccctgtcggc
     1621 aggcatgggg gttcccagca tggcaagacc acgacagacg ccaaccacag caacgcccgc
     1681 cagggtgccc aggaagcccg cggccaggaa gggccgggaa gcggcatcca aggcgcacac
     1741 ggccaagccg aatctcttcg atgacatcgc ccggcaagtc aacgccgacc aggaagccaa
     1801 ggaccgggca gccggccggg acgacctgac cccggcagtg gcgaaatccg tcgaccaggc
     1861 gacggtgaaa ccgtcgggca tcaatcgggc agcgacgagg ccaggcacgc cggcaaggcc
     1921 cgtgaagccc cctcggctga ccaagatcca ggcgaacggg aacggcgtga gcttcagtgg
     1981 tcggccgacg accaaggtcg aaacggtcgc cgctcgggtg aagaaggccg gcctcagggt
     2041 caggggcgat gccgacgtcg atgccgtcgc tgcccggctg cgggcgacgc ctggggccgt
     2101 gtcggcgtgg ctggcgaccc gcagcggtgc gccgaccaag ccccaggcga aggtcgggac
     2161 caagcaaccg gccacggcga agccgaggca gatgaaacct gacaaggcgc cgaccatcac
     2221 ccttggtcac ctctggggcg acaggcagcc ggactggctc taccgctgca ccctggtcac
     2281 ccgctacgaa gtccgcccgg atggaaccgt ctggagcacc ctggatcagg tgctcggccc
     2341 ggacggcaag gtggtcgagg acttctggcg cttaccgctg gatttcagac cgacccagca
     2401 gatccaaggc aacggccgga agctggtcct caaggaccgg accatcgccg gccttcagac
     2461 ctggctcatc cgagcaggct tgaccacgcc aagagacatc acgacataaa cgacctagca
     2521 cggctggcca gccagcagct cgcctggcct cgatgacctc ccagcgcacc accgccccac
     2581 cgtcaagggc accgaaggga cgagcgcagc gagcctgccc ttgacggcta cacggtgcac
     2641 gacggctgct ggttcgggga gggccgatcg atgagcgtgc cgggtacgcg aaaggcctag
     2701 gcagagatgc cgacgggtcg caggcggtgg gtaaccgcgc ccctgagcgg cattccagcg
     2761 gccaaatcgc acccccggtt cccggcgacc acccccgagg tctccggacc ccccggaaca
     2821 ccgatccttc cgctgtgtcc ccatcccctg ccggtgccgg cgggctcctc tgtgtgtccg
     2881 ctggacctct cgggaggact gcggtgaa
//
LOCUS       DASSWR010000192         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_883982,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000192 DASSWR010000000
VERSION     DASSWR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883982"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..249
                     /locus_tag="VFO47_03145"
                     /pseudo
     CDS             151..249
                     /locus_tag="VFO47_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
     gene            complement(402..1610)
                     /locus_tag="VFO47_03150"
     CDS             complement(402..1610)
                     /locus_tag="VFO47_03150"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8787726.1"
                     /translation="MNELSASGLADLAGVPEVEVARLVELGILVPRDGTGPFRETDVQ
                     KVRLAVACEQAGLPMAGIASAIRAGRLSFAFLEAAPFRRWAVRSTRTYRQVSQETGVP
                     LDTLGAVLESMGFARMGPDEPMREDELEVVPLLQLGLASGTFDLAWLARVGRAHVEGL
                     RLIATAWGEAYQARFEGPVLASGAGQGAAMERAAELSEGFLPLADPALLAIYRRQQEL
                     LWTEGLVERIEDELEQAGTLGRPGRVPAMSFLDLTGYTRLTEERGDAAAAALAETLAV
                     LVNRSSREHGGVPVKWLGDGVMIHHREPAGAVLSALGLVAQLPETGLPPAHVGVAAGP
                     VVAQGGDYFGRTVNLAARIAAHAGASQILVNGSVAESAPPQGVRFVEQGELPLKGFAH
                     PVRLLEACRA"
     gene            complement(1610..1702)
                     /locus_tag="VFO47_03155"
                     /pseudo
     CDS             complement(1610..1702)
                     /locus_tag="VFO47_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            complement(1756..2457)
                     /locus_tag="VFO47_03160"
     CDS             complement(1756..2457)
                     /locus_tag="VFO47_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787727.1"
                     /translation="MSALPNIVLAHGAWADGSSWSAVIQRLQADGYTVTAPQFPMTTL
                     ADDVARLRQALARQHGPTIVAGHSYGGQIITALGPDAPNVVGLVYIAAFGLDEGESIG
                     ALLSQTPGGPALAHLDIDPHGFAWLPEDDFVNHFAADVDPVTARVMFAVQQPLHWSAL
                     GEVMGVPAWRALPTWFLVAHGDQAIPPDAQRQFAARMGATTVEVPTNHVAMVSHPDEV
                     MQLIKTAAAAVPTAT"
BASE COUNT          333 a          929 c          895 g          354 t
ORIGIN      
        1 gtctccccag gccccacagc ctgggcgaga cggctggtcc cgcgactctt gctccgctcg
       61 ccctgcctat cgaacggcgg attcctcgtg ctgggttccg aacaggttgt aggaaccgac
      121 gacgtcaagg tgctcgatca ccggcggtcc gtgctgcacg acctgttcgc ggtgcccttt
      181 gacgagatcg gaccgatggt ggggcgctcc ccggcggcgg ctcgacagct cgcgagtcgg
      241 gcgccgcgat cgacgcgctc gctgacccgg agcggatcgg cgccctcgac ctcgggttcc
      301 tcgacgaccg gtccggctga gcggacggcg gggctgccgc tggcctgctt ggtctgctgc
      361 agccggcgag gcctcgcgag gcgaagccga ggcgtcagac gttatgcccg gcaggcctcc
      421 agcagccgca ccggatgggc gaaccccttg agtggtagct ccccctgctc cacgaagcgc
      481 acgccctggg gtggggccga ctcggccacg ctgccattga ccaggatctg gctggcgccg
      541 gcgtgggcgg cgatccgggc ggccagattg acggtgcggc cgaagtagtc gccgccctgg
      601 gcgaccaccg gcccggccgc caccccgaca tgggccggcg ggaggccggt ctcggggagc
      661 tgggccacca ggcccagagc cgacagcacc gccccggccg gctcccggtg gtggatcatc
      721 accccgtcgc ccagccactt cacgggcacg cccccgtgct cacgcgagga ccggttgacc
      781 agcacggcca gggtctcggc cagcgcggcg gcggccgcgt cgccccgctc ctcggtcagg
      841 cgggtgtagc cggtcaggtc caggaacgac atggcgggga cccgcccggg ccgcccgagc
      901 gtgccggcct gctccagctc gtcctcgatc cgctcgacca ggccctcggt ccacagcagc
      961 tcctgctggc gccggtagat ggccaacagg gccgggtcgg ccagcggcag gaagccctcg
     1021 ctcagctcgg cggcccgctc catggccgcc ccctggccgg cgcccgaggc cagcaccggc
     1081 ccctcgaacc gcgcctggta ggcctcgccc caggcggtgg cgatcagccg cagcccctcc
     1141 acgtgggccc ggccgacccg ggccagccag gccaggtcga aggtcccact ggccaggccc
     1201 agctgcagca gcggcaccac ctccagctcg tcctcccgca tcggctcgtc ggggcccatc
     1261 cgggcgaacc ccatcgactc cagcaccgcg ccgagcgtgt cgagcggcac cccggtctcc
     1321 tggctgacct ggcggtaggt ccgcgtcgag cgcactgccc agcgccggaa cggcgccgcc
     1381 tccaggaacg caaacgacag ccgccccgcc cggatcgccg aggcgatccc cgccatcggt
     1441 agcccggcct gctcgcacgc caccgccagg cggaccttct gcacatcggt ctcccggaac
     1501 gggccggtgc catccctggg caccaggatg ccgagttcga ccagccgcgc gacctcgacc
     1561 tcgggcacgc cggcgaggtc ggccagtccc gatgccgaca gctcgttcat gcggcgatcg
     1621 cctcgtggcc gtgctcggcc agcctcgcga cgaccttgga gccgatgagc ccggtgccac
     1681 cgatgacgac gacgttcgtc atgctggccc ttctatatgt tctgctcatc ggcaatgggc
     1741 tgatgctcgt tcaattcagg ttgcggtcgg caccgccgcg gcggcggtct tgatgagctg
     1801 catcacctcg tctgggtggg agaccatggc cacgtggttg gtcgggacct ccacggtggt
     1861 ggcgcccatg cgggcggcga actgccgctg ggcatcgggc gggatcgcct ggtcgccgtg
     1921 ggcgaccagg aaccaggtcg gcagcgccct ccaggccggg acgcccatca cctcccccag
     1981 ggcgctccag tgcagcggct gctggaccgc gaacatgacc ctggccgtga ccgggtcgac
     2041 atcggccgcg aagtggttga cgaagtcgtc ctcgggcagc caggcgaagc cgtgggggtc
     2101 gatgtcgaga tgggccaggg ccgggccccc tggtgtctgg ctcagcaggg cgccgatcga
     2161 ctcgccctca tccagcccga acgcggcgat gtagaccagc ccgacgacgt tgggggcgtc
     2221 ggggccgagg gcggtgatga tctggccgcc gtaggagtgg ccggcaacga tggttggccc
     2281 gtgctggcgg gccagggcct ggcgcagccg ggcgacgtca tcggccagcg tggtcatcgg
     2341 gaactggggt gcggtgacgg tgtagccgtc ggcctgcagg cgctggatga cggcgctcca
     2401 gctggacccg tcggcccacg cgccatgcgc caggacgatg ttcggtagcg cactcatctg
     2461 gttggctcct tccctgtggg cctgcgcgtg ctgggccatg gccagctgcg c
//
LOCUS       DASSWR010000193         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_205795,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000193 DASSWR010000000
VERSION     DASSWR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205795"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..651
                     /locus_tag="VFO47_03165"
     CDS             415..651
                     /locus_tag="VFO47_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787728.1"
                     /translation="MLSFGPFGVGGFVRLALDPGRVPVGRSVAPLAVAAFALADERFG
                     TGHRPAGAGPGGPLTEKGTRPQAGGASIGTCRAR"
     gene            complement(644..922)
                     /locus_tag="VFO47_03170"
     CDS             complement(644..922)
                     /locus_tag="VFO47_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET8787729.1"
                     /translation="MGIIGWIVLGLLAGIIAKAILPGNDPGGMIVTIIIGIVGAILGG
                     FLAQVLFGRNTVDEFFDISTWLTAIIGAIILLVIYRLAVGRGGRRRVI"
     gene            complement(996..1436)
                     /locus_tag="VFO47_03175"
     CDS             complement(996..1436)
                     /locus_tag="VFO47_03175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8787730.1"
                     /translation="MELSLALTLPRDEQTVSVARHIVRNAMEQVGVEETCVYDVELAL
                     SEACTNVLLHSGPGDQYVVRLDLDDRLGVIRVIDVGHGFDSAKLRAEDSLLEAERGRG
                     LGLMQGLVDRVDFTSRAEAGTIVTLEKVLTYDDEAFLVGRGKQV"
     gene            complement(1473..>2570)
                     /locus_tag="VFO47_03180"
     CDS             complement(1473..>2570)
                     /locus_tag="VFO47_03180"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HET8787731.1"
                     /translation="QRFRANLDGDESPDKSLDYCSACGLCTQACPEGVMVAEINQVAR
                     SRLVADRGGLNLRDQLVSRPSLMGAMARPVAPLANAVMAARPVRVLVEKTIGVHRGAA
                     MPKFSAMTFRSWARSHRDPPGATRTVAYFHGCAVQEFEPEVGAAVVRVLERNGVRVTV
                     PKQGCCGLPLVSNGDFDAGRSYATRLVRRLRSAADDPGVIVANSTSCGMTLKAKYREL
                     LGLDDADTKAVSQAVYDICEYLVGLDDEDALNRSFAPIQARALYHPPCQLMAHGVGTP
                     ALDLLALIPGLEMERSRAGCCGTAGTYGTKVEKYQIAMDVGQTLFDQVTESRPDFIVC
                     DSETCRWHIAQATGLPVYHPVQVLDRAYATA"
BASE COUNT          344 a          876 c          976 g          374 t
ORIGIN      
        1 gaggcggtgg ccttcctggc cgcccgctgc gaccgggtcc gggtgatgcc gttcctgcag
       61 ggcatcccgt gcagcatcca cgggtggtgt tcccccgcgg ggtggccacc ttccgcccgg
      121 tcgagatggt gaccctgcgc cggccggggg gtccggggga ccgccggggt ggtcccccgg
      181 agaatcgggg gtcgaaccgc ctgcggtacg cgggggcggc caccttctgg gacccgcccg
      241 ccgccgaccg ggaggtcctg cgcgacctgg cccgccgggt cggggccggg ttgcgcgagc
      301 gggtcggcta ccggggcgcc ttcaccgtcg acggggtgct ggcggccgag gggttcctgc
      361 ccaccgacgg gcgggacctg ccggccggcc gaggacgggc ggcgggccga cggggtgctg
      421 tcgttcgggc cgttcggggt cggcgggttc gtgcgcctgg ccctcgaccc gggtcgggtg
      481 ccggtgggcc gttcggtggc gccgctcgcc gtggccgcct tcgccctggc cgacgagcgc
      541 ttcggcaccg gtcatcggcc cgctggagcc ggcccgggcg gtccgctgac ggagaagggg
      601 acacgccccc aggccggggg cgcgtcgatt gggacgtgcc gggctagatg acccggcgcc
      661 tgcctcctcg gccgacggcg aggcggtaga tcaccagcag gatgatggcg ccgatgatcg
      721 ccgtcagcca cgtgctgatg tcgaagaact cgtccaccgt gttacggccg aacaagacct
      781 gggccaggaa cccgccgagg atcgcgccca cgatgccgat gatgatggtc acgatcatcc
      841 cgcccgggtc gttgccgggc aggatcgcct tggcgatgat cccggcgagt aggccgagca
      901 cgatccagcc gatgatgccc attccacgtc ctttcgtcgc ttcggcggca tgttgccgcc
      961 cttgacagca ctggaatgag cgatacgagc cgggttcaaa cctgctttcc gcgccccacc
     1021 aggaacgcct cgtcgtcgta ggtcaggacc ttctcaaggg tgacgatcgt ccccgcctcg
     1081 gcccgggagg tgaagtcgac ccggtcgacc aggccctgca tcagccccag gccacggccc
     1141 cgctcggcct cgagcaggga gtcctcggcc cgcagcttgg ccgagtcgaa gccgtggccg
     1201 acgtcgatca cccggatcac gccgaggcgg tcatccaggt cgaggcggac gacgtactgg
     1261 tcgccgggac cggagtggag cagcacattg gtgcaggcct cgctcaaggc cagctcgacg
     1321 tcatagacgc aggtctcctc cacaccgacc tgctccatgg cgttgcggac gatgtgtcgg
     1381 gccaccgaga ccgtctgctc gtcccgtggc agggtcaggg ccagcgagag ttccatgccg
     1441 gtgtcgttcc cttcgagaag ggagccgaca cctcaggccg tggcatacgc ccggtcgagc
     1501 acctggaccg ggtggtagac gggcaggccg gtcgcctggg cgatgtgcca gcggcaggtc
     1561 tcggagtcgc agacgatgaa gtcgggccgg gactcggtga cctggtcgaa cagggtctgc
     1621 ccgacgtcca tggcgatctg gtacttctcg accttggtgc cgtaggtgcc ggcggtgccg
     1681 cagcagccgg cccgcgaccg ctccatctcc agtcccggga tcagggcgag caggtcgagg
     1741 gcgggggtgc cgacgccgtg ggccatgagc tggcagggcg ggtggtacag ggcccgggcc
     1801 tggatggggg cgaacgaccg gttcagcgcg tcctcgtcgt ccagcccgac caggtactcg
     1861 cagatgtcgt agacggcctg ggagaccgcc ttggtgtcgg cgtcgtccag ccccagcagc
     1921 tcccggtact tggccttgag ggtcatgcca cacgaggtcg agttggccac gatcaccccg
     1981 gggtcgtcgg ccgcgctccg cagccgcctg accagcctgg tggcgtagct ccggccggcg
     2041 tcgaagtcgc cgttggagac cagcggcagc ccgcagcagc cctgcttggg gacggtcacc
     2101 cggacgccgt tgcgctccag gacccggacg acggccgcgc ccacctcggg ctcgaactcc
     2161 tggacggcgc agccgtggaa gtaggcgacg gtgcgggtgg cgccgggcgg gtcgcggtgg
     2221 gagcgggccc aggagcggaa ggtcatggcc gagaacttgg gcatggccgc gccccggtgc
     2281 acgccgatgg tcttctcgac cagcacccgg accggccggg cggccatgac ggcgttggcc
     2341 aggggcgcca ccggccgggc catggcgccc atcagcgagg gccggctgac cagctggtcg
     2401 cgcaggttga ggccgccgcg gtcggcgacc aggcggctgc gggcgacctg gttgatctcg
     2461 gccaccatca cgccctctgg gcaggcctgg gtgcagaggc cgcaggccga gcagtagtcg
     2521 agggacttgt cgggagactc gtcgccgtcg aggttggccc ggaaccgctg
//
LOCUS       DASSWR010000194         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1279617, whole genome shotgun sequence.
ACCESSION   DASSWR010000194 DASSWR010000000
VERSION     DASSWR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279617"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /gene="hutI"
                     /locus_tag="VFO47_03185"
     CDS             complement(<1..985)
                     /gene="hutI"
                     /locus_tag="VFO47_03185"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET8787732.1"
                     /translation="MRADFLLTGAGQLVTCDPALGEGPLGLAERRAVAAAGGRVVFAG
                     PEAALPDLEVDAGAVRVDAGGRAVLPGFVDAHTHLVFAGERAEEFAARLRGVGYEEAL
                     AAGGGINRTVRDTRAASDAELEASAGRRLATALAYGTTTLEAKSGYGLTVADERRSLE
                     VLARLAERSPVEVVPTFLGAHLIPEEYTGDRGGYLDLLEHEMLPACAPLAEFVDAFCD
                     RGALTVEESRRVLVAGARHGLRTKLHANELGSTGGAALAAELGCVSADHLLFCDHQEA
                     KALAAAGTVAVLLPGTSFLLRTGRAAPAPVLRDAGVRMALGTDCNPGTCYSE"
     gene            complement(982..2649)
                     /gene="hutU"
                     /locus_tag="VFO47_03190"
     CDS             complement(982..2649)
                     /gene="hutU"
                     /locus_tag="VFO47_03190"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET8787733.1"
                     /translation="MSIGTAARTVRAPRGTTLSCRGWQQEAALRMLCNNLDPQVAERP
                     GDLVVYGGTGRAARDWAAFDRIVATLRRLGDDETLVVQSGKPVGVFRTHSFAPRVLLA
                     NSLLVPEWATWDRFRELEAAGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVARKNGW
                     DSLRGKVVLTAGLGGMGGAQPLAVTMNEGVALCVEVDPERLARRLDTGYLDERADDLA
                     DATARVERAAAEGRAVSVGLVGNAADVFPELLAAGFRPDVVTDQTSAHDPLGGYVPSG
                     IEPGDAPAARAGDPTGYVKQARESMARHCEAMVGFQRAGAEVFDYGNNLRGEARVAGF
                     GDAFAYPGFVPAYIRPLFCEGKGPFRWAALSGDPADIVRTDRAVLELFGDDEHLRRWL
                     SMAAERVRFQGLPARICWLGYGERHQAGLAFNDLVARGEVQAPIVIGRDHLDSGSVAS
                     PYRETEAMADGSDAIADWPILNALLNTASGASWVSVHHGGGVGIGRSIHAGVVVVADG
                     STEAARRVELVLTNDPGTGVMRHADAGYDQARQVAAERGMDLPGITT"
BASE COUNT          341 a         1024 c         1041 g          325 t
ORIGIN      
        1 actcgctgta gcaggtcccc gggttgcagt cggtgccgag ggccatcctc accccggcgt
       61 cccgcaggac cggggccggg gcggcgcggc cggtgcggag caggaagctg gtcccgggca
      121 gcagcaccgc caccgtgccc gcggcggcca gcgccttggc ctcctggtgg tcgcagaaca
      181 gcaggtggtc ggctgacacg cagcccagct cggcggccag ggcggccccg ccggtcgagc
      241 ccagctcgtt ggcgtggagc ttggtcctga ggccgtggcg ggcgccggcg accaggaccc
      301 gccgggactc ctcgacggtg agggcccccc ggtcgcagaa cgcgtccacg aactcggcca
      361 ggggggcgca ggccggcagc atctcgtgct cgagcaggtc caggtagccg ccgcggtctc
      421 ccgtgtactc ctcggggatc aggtgggcgc cgaggaaggt cgggaccacc tccaccgggg
      481 accgctcggc cagccgggcc agcacctcca ggctgcggcg ctcgtcggcg acggtgaggc
      541 cgtagccgga cttggcctcc agggtggtgg tgccgtaggc cagggcggtg gccagcctcc
      601 ggcccgcgga cgcctccagc tcggcgtcgg aggcggcccg ggtgtcgcgg accgtgcggt
      661 tgatgccgcc cccggcggcc agggcctcct cgtagccgac cccgcgcagc cgggcggcga
      721 actcctcggc ccgctcgccg gcgaacacca ggtgggtgtg ggcgtccacg aacccgggca
      781 gcaccgcccg gccgccggcg tcgacccgga ccgcgccggc gtccacctcc aggtcgggca
      841 gggccgcctc gggcccggcg aacaccaccc gccccccggc ggcggcgacc gcccgccgtt
      901 cggccagccc gagcggcccc tcccccagcg ccgggtcgca ggtgacgagc tggccggccc
      961 cggtgagcag gaagtcggcc ctcacgtggt gatcccggga aggtccatgc cccgctcggc
     1021 ggcgacctgg cgggcctggt cgtagccggc gtcggcgtgg cgcatgacgc cggtgccggg
     1081 gtcgttggtc aggaccagct cgacccgccg ggcggcctcg gtggagccgt cggccaccac
     1141 caccacgccg gcgtggatgg agcggccgat accgaccccg ccgccgtggt ggacgctcac
     1201 ccaggaggca cccgaggccg tgttgagcag ggcgttcagg atcggccagt cggcgatggc
     1261 gtcggagccg tcggccatgg cctcggtctc ccggtagggg ctggccaccg acccggagtc
     1321 gaggtggtcg cggccgatca cgatcggggc ctggacctcg ccccgggcca ccaggtcgtt
     1381 gaaggccagc ccggcctggt ggcgctcgcc gtagccgagc cagcagatcc gcgccggcag
     1441 cccctggaag cggacccgct cggcggccat gctcagccag cggcgcagat gctcgtcgtc
     1501 gccgaacagc tccagcacgg cccggtcggt gcggacgatg tcggccgggt cgccggacag
     1561 ggcggcccag cggaacggcc ccttgccctc gcagaacagc ggccggatgt aggcgggcac
     1621 gaaccccggg taggcgaagg cgtccccgaa cccggccacc cgggcctcgc cgcgcaggtt
     1681 gttgccgtag tcgaacacct cggccccggc ccgctggaag ccgaccatgg cctcgcagtg
     1741 gcgggccatc gactcccggg cctgcttgac gtagccggtc gggtccccgg cccgggccgc
     1801 gggggcgtcg ccgggttcga tccccgaggg gacgtagccg ccgagagggt cgtgggccga
     1861 ggtctggtcg gtaaccacgt cgggccggaa accggcggcc agcagctcgg ggaagacgtc
     1921 ggcggcgttg ccgaccagcc caaccgacac cgcccggccc tcggcggcgg cccgctcgac
     1981 cctcgcggtg gcgtcggcca ggtcgtcggc ccgctcgtcc aggtagccgg tgtccaggcg
     2041 ccgggccaga cgctcggggt cgacctcgac gcagagggcg acgccctcgt tcatggtcac
     2101 ggccagcggc tgggccccgc ccatgccgcc gaggccggcg gtgagcacga ccttgccgcg
     2161 gaggctgtcc caaccgttct tccgggccac ggccgcgaag gtctcgtagg tgccctggag
     2221 gatgccctgg gtgccgatgt agatccagga cccggcggtc atctggccgt acatggtgag
     2281 cccggccgcc tccagctcgc ggaaccggtc ccaggtggcc cactcgggca ccagcaggga
     2341 gttggcgagc agcacccggg gggcgaagct gtgggtccgg aacacgccca ccggcttgcc
     2401 cgactggacc accagggtct cgtcgtcgcc gaggcgccgc agggtggcca cgatccggtc
     2461 gaacgccgcc cagtcccgcg ccgcccgccc ggtgccgccg tagacgacca ggtcaccggg
     2521 ccgctcggcg acctgggggt cgaggttgtt gcagagcatc cgcagcgcgg cctcctgctg
     2581 ccagccccgg caggagaggg tggtaccgcg aggggcgcgc accgtccggg cggccgtgcc
     2641 gatgctcatc ggcgtgatga tacgcacccg gcgctcagcc gacgggcggg agctggccga
     2701 gcgtgacgct gatctctcgc tcctcgccgt c
//
LOCUS       DASSWR010000195         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_318943,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000195 DASSWR010000000
VERSION     DASSWR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318943"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFO47_03195"
     CDS             <1..337
                     /locus_tag="VFO47_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET8787734.1"
                     /translation="CDRYLDSSLAYQGGARGLGRGPVTEVNRVATGGLLPDLVVLLDL
                     DPAAGLARRPRDPDRIEAQDLGFHRRVRDAFRDLAAAEPGRFAVVDAAAPVEEVADQI
                     RAAVLGLLT"
     gene            334..1536
                     /gene="holB"
                     /locus_tag="VFO47_03200"
     CDS             334..1536
                     /gene="holB"
                     /locus_tag="VFO47_03200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET8787735.1"
                     /translation="MSDPSIWDLVVGHDEAVAMLKEAVASERVTHAWLFTGPPGIGKL
                     HTARVFAAALNCPAGGDGTCDTCRRILRGVHPDVHLIVPEGDNLLVEDVRAVREEASR
                     THHEAAVAVFILDEADRLTEAAANALLKVLEEPPVDVVFVLVARSAEALVGTVPSRAR
                     TLPFVSLSLAELTAALGDDLQLDPAQTTWAAAASHGRLARARALLTDEAARTRRSTVL
                     DLTERLASGQPSDALAAAATVVAIADEVAAAARLRQERELAELEEAFGTGRGTGALRK
                     RLETRHRRELRRVRFDAIRDGVADLLGAYRDIALLSGGSGGEDPGPGSPGVAPLVHPD
                     KAGSFGRLAARTDPASALRAAGALEEADRRLAIGAAPLLTLEAAFLSAQAALRGPAVP
                     INRLAIRR"
     gene            1599..2051
                     /locus_tag="VFO47_03205"
     CDS             1599..2051
                     /locus_tag="VFO47_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HET8787736.1"
                     /translation="MAAGRRNGPSLFWPGVLTGIGLAGTLDEVVLHQLLGWHHFYDRS
                     TATVGLVSDGIFHLFSTAVLVLGAIQLVERRRTSPDPPRLALAGILLGAGGFNLYDGT
                     IQHKLLRLHQIRPAAPNDLPYDLVFLTIAAALALSGSLLLRRTRQPST"
     gene            2128..2199
                     /locus_tag="VFO47_03210"
     tRNA            2128..2199
                     /locus_tag="VFO47_03210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2160..2162,aa:Thr,seq:cgt)
BASE COUNT          267 a          974 c          688 g          295 t
ORIGIN      
        1 gtgcgaccgc tacctcgact cctccctcgc ctaccagggc ggcgcccgcg ggctcggccg
       61 gggcccggtc accgaggtca accgggtcgc caccggcggg ctcctgcccg acctggtggt
      121 gctgctcgac ctcgacccgg ccgccggcct ggcccgccga ccccgcgacc ccgaccggat
      181 cgaggcccag gacctcggct tccaccgccg ggtccgggac gccttccgcg acctggccgc
      241 cgccgagccc ggacggttcg ccgtggtcga cgccgccgcc ccggtagaag aggtcgccga
      301 ccagatccgg gccgccgtcc tcgggctgct gacatgagcg acccctcgat ctgggacctg
      361 gtcgtcgggc acgacgaggc cgtggccatg ctcaaggagg cggtggcctc cgagcgggtc
      421 acccacgcct ggctgttcac cggtcctcca gggatcggca agctccacac cgccagggtg
      481 ttcgccgccg ccctgaactg cccggccggc ggcgacggca cctgcgacac ctgccggcgg
      541 atcctccgcg gcgtccaccc cgacgtccac ctgatcgtcc ccgagggcga caacctcctg
      601 gtcgaggacg tccgggccgt ccgcgaggag gcctcccgca cccaccacga ggccgcagtg
      661 gccgtgttca tcctcgacga ggcggaccgg ctcaccgagg ccgccgccaa cgccctcctc
      721 aaggttctcg aggagcctcc cgtggacgtg gtcttcgtcc tggtcgcccg cagcgccgag
      781 gccctggtcg gcaccgtccc ctcccgggcc cgcaccctgc cgttcgtgag cctgtcgctg
      841 gccgagctga ccgccgccct cggcgacgac ctccagctcg accccgccca gaccacctgg
      901 gccgccgcgg ccagccacgg ccgcctggcc cgggccaggg ccctcctcac cgacgaggcc
      961 gcccgcaccc gccgctccac cgtcctcgac ctcaccgagc gcctcgccag cggccagccc
     1021 tccgacgccc tggccgccgc cgccaccgtg gtcgccatcg ccgacgaggt cgccgccgcc
     1081 gcccgcctcc gccaggaacg cgagctggcc gagctggagg aggccttcgg taccggccgc
     1141 ggcaccggcg cgttgcggaa acggctcgag acccgacacc gccgcgagct ccgccgggtc
     1201 cggttcgacg ccatccgcga cggggtcgcc gacctgctcg gggcctaccg ggatatcgcc
     1261 ctgctcagcg gtgggtcggg tggtgaggac ccggggccgg ggtcgcccgg ggtggcaccc
     1321 ctggtgcacc cggacaaggc cggttcgttc gggcgcctgg ccgccaggac cgacccggcc
     1381 agcgccctcc gcgccgccgg cgccctggag gaggccgacc gccgtctggc catcggcgcc
     1441 gcccccctcc tgacccttga ggccgccttc ctctccgccc aggcggcact gcgcggcccg
     1501 gccgttccca tcaaccgcct cgccatccgc cgctgacgcc cggtttgcgc cccgggatgc
     1561 cgtgggaccc gcccctcgac ggggaggagg tggtgggcat ggcagccgga cggaggaacg
     1621 ggccgtccct gttctggccc ggcgtcctga ccggcatcgg cctcgccggc accctcgacg
     1681 aggtcgtcct ccaccagctc ctcggctggc accacttcta cgaccgctcg accgccaccg
     1741 tcggtctcgt ctccgatggc atcttccacc tcttcagcac cgccgtcctc gtcctcggcg
     1801 ccatccagct ggtcgaacgc cgccgcacgt cccccgaccc accccgcctc gccctcgccg
     1861 gcatcctcct cggcgccggc ggcttcaacc tctacgacgg caccatccag cacaagctcc
     1921 tccgcctcca ccagatccgc cccgccgccc ccaacgacct cccatacgac ctcgtcttcc
     1981 tcaccatcgc cgccgccctc gccctcagcg gctccctcct cctccgccgc acccgccagc
     2041 ccagcacctg aaccgatctc cctggcccac gcccaacgcc cttcggtaca ctccgccttc
     2101 gcaccgacca gttcgccgtg cccgccagcc cgagtggcgt agtggtagcg catccgactc
     2161 gtaatcggaa ggtcgtcggt tcgaatccga cctcgggctc ccaaaccccc tggtgagagc
     2221 gttg
//
LOCUS       DASSWR010000196         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_177795,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000196 DASSWR010000000
VERSION     DASSWR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177795"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1219
                     /gene="dnaJ"
                     /locus_tag="VFO47_03215"
     CDS             86..1219
                     /gene="dnaJ"
                     /locus_tag="VFO47_03215"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET8787737.1"
                     /translation="MNNPEYLEKDFYAALGVSKDASAADIKKAYRKLAQQHHPDANKG
                     DKASEERFKEVGRAYAVLSDPKKRAEYDEARRLLSSGAFGGGGFGGFPGGGGGFPGGR
                     VRVEDLGGLGDLFGNLFGGGRPSQTAARRGRDVESEVTLGFEEAIRGATIPLQLRGPA
                     PCHTCMGSGAKPGTLPRPCPTCNGRGTVTRDQGLFGLSSPCPTCQGRGSVIDDPCPTC
                     NGRGSEVRTRELRVRIPTGVNDGATIRLKGQGEPGQAGAPAGDLIVKVRVTPHPIFGR
                     NGRDLTITVPVTFAEAALGTELKVPTLDGPVTLKVPAGTQNGRTFRVRGRGVPGAGRK
                     GAGDLMVSLQVAVPEKLSRKERDLLREFASLSDTTPRNHLGID"
     gene            1237..1647
                     /locus_tag="VFO47_03220"
     CDS             1237..1647
                     /locus_tag="VFO47_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019524250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8787738.1"
                     /translation="MAEQRDRAVFVISVAAELAGVHPQTLRIYEREGLVRPKRTSANA
                     RRYSERDIERLLEIKRLTGDGMNLEGVRRVIALTQEIERLQATVAALESELRTQARRH
                     RQEVESVRRSARRDLVPYSSAIVRYEHPARYEQA"
     gene            1668..>2661
                     /locus_tag="VFO47_03225"
     CDS             1668..>2661
                     /locus_tag="VFO47_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET8787739.1"
                     /translation="MDVNSFTHRAAEALATAQREARSNDHQRIEPEHLLVALLADPDG
                     VVYPVVRRTGADPGMLKARAAEALDRIPKVYGPEQETVASPQLGRLIERARKEAGALG
                     DQYVSTEHLLLALTEGSTAAAKLLTGAGVTREAILAALTEVRGGKRVTDQDPEAKYQA
                     LERFGRDLTEMARQGKLDPVIGRDDEVRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAGRIVAGDVPESLKDKRIVALDLGAMVAGSKFRGEFEERLKAVLDDIRGSEGQIITF
                     IDELHTVVGAGAAEGAMDAGNMLKPMLARGELRMIGATTLDEYRERIEKDPALER"
BASE COUNT          415 a          981 c          959 g          306 t
ORIGIN      
        1 tggtcgtcga catcgtgcgg gccggctacg agctgcgggg caaggtcctg cgccccgcca
       61 tggtgaaggt tgcgaagtag cggagatgaa caaccccgag tacctggaga aggacttcta
      121 cgccgccctc ggcgtgtcca aggacgcgtc ggcggccgac atcaagaagg cctaccggaa
      181 gctcgcccag cagcaccacc cggacgccaa caagggtgac aaggcctccg aggagcgctt
      241 caaggaggtc gggcgggcct atgccgtgct gtccgacccc aagaagcggg ccgagtacga
      301 cgaggcccgg cggctgctgt cctcgggggc gttcgggggc ggcggcttcg gcggcttccc
      361 gggcggcggg ggcgggttcc ccggcggccg ggtccgggtc gaggaccttg gtggcctcgg
      421 cgacctgttc ggcaacctgt tcggcggcgg ccgcccgtcc cagacggcgg cccggcgggg
      481 ccgcgacgtc gagagcgagg tcaccctcgg cttcgaggag gcgatccgcg gggccaccat
      541 cccgctgcag ctgcgcggcc cggcgccctg ccacacctgc atggggtcgg gggccaagcc
      601 cggcaccctg ccccggccct gcccgacctg caacgggcgg ggcacggtca cccgcgacca
      661 gggcctgttc ggcctctcca gcccctgccc gacctgccag ggccgcggct cggtgatcga
      721 cgacccctgc ccgacctgca acggccgcgg cagcgaggtc cgcacccggg aactgagagt
      781 aaggatcccc accggggtca acgacggggc caccatccgg ctcaagggcc agggcgagcc
      841 cggccaggcc ggcgcccccg ccggcgacct gatcgtcaag gtgcgggtca ccccgcaccc
      901 catcttcggg cgcaacggcc gggacctgac catcaccgtg ccggtgacct tcgccgaggc
      961 cgccctcggc accgagctca aggtgccgac cctggacggc ccggtgacct tgaaggtgcc
     1021 ggccgggacc cagaacggcc gcaccttccg ggtccggggg cgcggcgtcc ccggggccgg
     1081 ccgcaagggc gccggcgacc tgatggtcag tctccaggtg gccgtgcccg agaagctctc
     1141 gcgcaaggag cgggacctgc tgcgggagtt cgcgtcgctg tccgacacga cgcccaggaa
     1201 ccacctcggg atcgactgag cgaggggcgc tgaaccatgg cggagcaacg ggaccgggcg
     1261 gtgttcgtga tctcggtcgc ggccgagctc gccggggtcc acccccagac gctgcggatc
     1321 tacgagcgcg agggcctggt ccggcccaag cggaccagcg ccaacgcccg ccgctactcc
     1381 gagcgggaca tcgagcggct gctggagatc aagcggctga ccggcgacgg catgaacctg
     1441 gaaggggtgc ggcgggtgat cgcgttgacc caggagatcg agcgtctcca ggccaccgtg
     1501 gccgcgctcg agtccgagct gcggacccag gcccgccgcc accgccagga ggtcgagagc
     1561 gtccggcggt cggcccggcg cgacctggtc ccctactcct cggccatcgt ccgctacgag
     1621 catcccgcac gatacgagca ggcctgaacc tgaaacggaa catcaccatg gacgtgaaca
     1681 gcttcaccca ccgggccgcc gaggcgctgg ccaccgccca gcgcgaggcc cgctccaacg
     1741 accaccagcg gatcgagccc gagcacctgc tggtcgcgct gctcgccgac cccgacgggg
     1801 tcgtgtaccc ggtcgtgcgc cggaccggcg ccgacccggg catgctcaag gccagggccg
     1861 ccgaggccct ggaccggatc cccaaggtct acggccccga gcaggagacg gtggcctcgc
     1921 cccagctggg ccgcctgatc gagcgggccc gcaaggaggc cggcgccctc ggcgaccagt
     1981 acgtctccac cgagcacctg ctgctcgccc tcaccgaggg ctccaccgcc gcggccaagc
     2041 tgctcaccgg cgcgggcgtg acccgcgagg ccatcctggc cgccctgacc gaggtccgcg
     2101 ggggcaagag ggtcaccgac caggaccccg aggccaagta ccaggccctg gagcgcttcg
     2161 gccgcgacct gaccgagatg gcccgccagg gcaagctcga cccggtgatc ggccgcgacg
     2221 acgaggtccg ccgggtcgtc caggtgctga gccgccggac caagaacaac ccggtgctga
     2281 tcggcgagcc cggcgtcggc aagacggcca tcgtcgaggg cctggccggc cggatcgtgg
     2341 ccggggacgt gcccgagtcg ctcaaggaca agcgcatcgt cgccctcgac ctgggagcca
     2401 tggtcgccgg gtcgaagttc cggggcgagt tcgaggagcg gctcaaggcc gtgctcgacg
     2461 acatccgcgg ctccgagggc cagatcatca ccttcatcga cgagctccac accgttgtcg
     2521 gggccggcgc cgccgagggc gccatggacg ccggcaacat gctcaagccg atgctggcgc
     2581 gcggcgaact gcgcatgatc ggcgcgacca cgctggatga gtaccgcgag cggatcgaaa
     2641 aggaccccgc cctggagcgg c
//
LOCUS       DASSWR010000197         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_573344,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000197 DASSWR010000000
VERSION     DASSWR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573344"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..884)
                     /locus_tag="VFO47_03230"
     CDS             complement(18..884)
                     /locus_tag="VFO47_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787740.1"
                     /translation="MPDRRPGGRPSEGAVLWRQVRAQNKLFLRNPFSAFFSLAFPVMF
                     LLLFGSLNGGGRIEELGGIRYIQFLAPGMLAFAVISTCYTGLVTGVAINRDEGLLKRV
                     RGTPLPPWVYISARIGSAVWFSMVSAVVMVVAAVLLFRVEVIGRMLPAAVVTLLGGAA
                     CFCALGMAVAAVIPNGEAAPVIANFTFFPIAFVSDLFFPTAGAPAWVGTVGSIFPMKH
                     FAVAMGHTFNPLAAGSGFQWGHLGMLALWTVAGVVVAVRFFRWEPRTGAPRGGRRRAG
                     RAGRVAEADTAA"
     gene            complement(877..1803)
                     /locus_tag="VFO47_03235"
     CDS             complement(877..1803)
                     /locus_tag="VFO47_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787741.1"
                     /translation="MNTAIRVTGLRKRYGELEAVRGIDFEVAEGECFALLGPNGAGKT
                     TTVEILEGYRDRDAGDVEVLGLDPRRGGRDLRERIGIVLQSSGHFRELTVREVLELFG
                     GYYPRPRATGEVIDLVGLGEKAGARVKTLSGGQQRRLDLALGLVGDPDLVFLDEPTTG
                     FDPSARRRSWELIENLRELGKTILLTTHYMDEAQNLADRVAIIVAGRIVAAGTPDSLG
                     GRDEGEAVISFRLPAGMTLTDLPADLPGRPAPREDRVELRTTEPTGALNVLTSWALAR
                     GEELDALTVTRPSLEDVYLQLTGAEEQEPAHA"
     gene            1790..2320
                     /locus_tag="VFO47_03240"
     CDS             1790..2320
                     /locus_tag="VFO47_03240"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HET8787742.1"
                     /translation="MAVFMVDPNCSLRGVNQGPEPEDVRAAAVAAVAAFVADAGVDAE
                     ELGDGRWFLRLAGERKLGIGVHLAVGDRTLRVESFFMRAPEEQRERLYRDLLLRQATS
                     YVLRFTLDEAGDLFLVGQVPLRAVTPEEVDRVVGSVLELADAAYLPAVEIGFASSIAA
                     EKAWRARLAEGSARPP"
     gene            2774..3991
                     /locus_tag="VFO47_03245"
     CDS             2774..3991
                     /locus_tag="VFO47_03245"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET8787743.1"
                     /translation="MSITLTQESVVTEMARPEGSKALLLVMDGLGGYRTRERGSELTE
                     ASTPNLDRLAGVGSLGLHDPVTRGITPGSGAGHLGLFGYDPLAYEIGRGALSAAGIGF
                     ELQPGDVAARVNFCTINPDGTIADRRAGRLPTEEAAGICDLLVREIQLDGVQFFLQPE
                     REHRGLLVLRGPGLDRDVTDTDPQREGVPPLDPVARSPEAKRTAELIGRILDQARTLL
                     ADRERGNFLLLRGFDSVKEMPSFPDRYQVRALAIAAYPMYVGISRLLGFQTHQVDGAA
                     PEEVDALAELLPDHDFVFMHIKKTDSAGEDGDFERKVQAIEEVDALIPRMLDAGVDVL
                     AVTGDHSTPAQMAAHSWHPVPVLLWGGTAAADGLPAFDEVTCRQGSLGRFEAKHLLPQ
                     LLAAAGRLTKYGA"
BASE COUNT          586 a         1513 c         1438 g          519 t
ORIGIN      
        1 ccggtcccgg aggaaggcta ggccgccgtg tcggcctcgg cgacccgccc ggcccggccg
       61 gcccggcggc ggccgccgcg cggcgccccg gtgcgcggct cccagcggaa gaagcggacg
      121 gccacgacca ccccggcgac cgtccacagg gccagcatgc ccaggtgccc ccactggaac
      181 ccgctgccgg ccgccagcgg gttgaaggtg tgccccatgg ccacggcgaa gtgcttcatc
      241 gggaagatcg agccgaccgt gcccacccag gcgggggcgc cggcggtggg aaagaacagg
      301 tcggagacga aggcgatggg gaagaaggtg aagttggcga tcaccggcgc cgcctccccg
      361 ttggggatga ccgcggccac cgccatgccc agggcacaga agcaggccgc cccacccagc
      421 agggtcacga ccgcggcggg cagcatccgg ccgatcacct cgacacggaa cagcagcacg
      481 gccgcgacca ccatcaccac cgcgctcacc atcgagaacc acaccgccga gccgatgcgg
      541 gcgctgatgt agacccacgg cggcaggggc gtgccccgga cccgcttgag caggccctcg
      601 tcgcggttga tggccacccc ggtgacgagc ccggtgtagc aggtggagat caccgcgaag
      661 gcgagcatgc ccggggcgag gaactggatg tagcggatgc cgcccagctc ctcgatgcgg
      721 ccgccgccgt tgagggagcc gaacagcagc aggaacatca ccgggaaggc gagcgagaag
      781 aaggccgaga acgggttgcg gaggaacagc ttgttctggg cgcggacctg gcgccagagc
      841 acggccccct cggaggggcg gcccccgggg cggcggtcag gcatgggccg gctcctgttc
      901 ctcggcgccg gtgagctgga ggtagacgtc ctccagcgac ggcctggtga cggtcagcgc
      961 gtccagctcc tccccccggg ccagcgccca ggaggtgagg acgttgaggg cgccggtggg
     1021 ctcggtggtg cgcagctcga cccggtcctc acgcggcgcc ggccggcccg gcaggtcggc
     1081 cggcaggtcg gtcagggtca tgccggccgg gaggcggaag gagatcaccg cctcgccctc
     1141 gtcgcggccg cccagcgagt cgggggtgcc ggcggccacg atccgcccgg ccacgatgat
     1201 ggccacccgg tcggccaggt tctgggcctc gtccatgtag tgggtggtga gcaggatcgt
     1261 cttgccgagc tcgcggaggt tctcgatcag ctcccaggag cgccgcctgg ccgacgggtc
     1321 gaacccggtg gtcggctcgt ccaggaagac caggtccggg tcgccgacca gcccgagggc
     1381 caggtcgagc cgccgctgct ggccgcccga gagcgtcttg acccgggcgc cggccttctc
     1441 gcccaggccg accaggtcga tcacctcgcc ggtggcccgc gggcgcgggt agtagccgcc
     1501 gaacagctcc agcacctcgc gcacggtcag ctcgcggaag tggccggagg actggagcac
     1561 gatcccgatc cgctcgcgca ggtcccgccc gccccgccgc gggtcgaggc cgagcacctc
     1621 gacgtcgccg gcgtcccggt cccggtaccc ctcgaggatc tcgacggtgg tcgtcttgcc
     1681 ggccccgttc ggccccagca gcgcgaagca ctccccctcg gcgacctcga agtcgatgcc
     1741 gcggacggct tccagctcgc cgtagcgctt gcgcaggccg gtgacgcgga tggctgtgtt
     1801 catggtcgac cccaactgta gtctcagggg cgtgaaccag ggacccgagc ccgaggacgt
     1861 gcgggcggcg gcagtggccg cggtggcggc cttcgtcgcc gacgccggcg tggacgccga
     1921 ggagctgggc gacgggcgct ggttcctgcg cctggccggc gagcgcaagc tcgggatcgg
     1981 cgtccacctg gcggtggggg accggaccct gcgggtggag tcgttcttca tgcgggcccc
     2041 cgaggagcag cgcgagcggc tctaccgcga cctgctgctc cgccaggcga ccagctacgt
     2101 cctccggttc accctggacg aggccggcga cctgttcctg gtcggccagg tcccgctgcg
     2161 ggcggtcacc cccgaggagg tcgaccgggt ggtcggctcg gtcctggaac tcgccgatgc
     2221 cgcctatttg cccgcggtgg agattgggtt cgcatcgagc attgcagccg aaaaagcctg
     2281 gcgggcccgg ctggccgagg gttccgcccg tccgccgtaa attccatcga agtaattcta
     2341 catgcctcta gcagcacata cgcgtctgac cagcggaaac ggtgccttga cggacccctt
     2401 gccgccttct agattccgct tcgccccgag gggccgacgc ggtaggactg ggaaatccgg
     2461 atcggagttc tcgccagaag ggctgacggg cccggacggc gcgtcgcggg cggtgggacg
     2521 cgcaacccgc cgactgcggc gaggtccaag cggcctctgg gtcgtaggac gggactttcg
     2581 cgagtcgttc tcgggaaggc cacgggagtt cctcggggcg ccgatgaccc ccaggggccc
     2641 aatcggccgg gagcaggcgg gcttgcccgc cggtcaaccg gacggggttc ctgggggtcg
     2701 tcacttttcg cgcccgcccg gcggggaacc gtagactcag gtccagtacc ccccggcagg
     2761 tcggaggcgg cccatgtcga tcaccctcac gcaagagtcc gtcgtgaccg agatggcgag
     2821 gcccgaaggc agcaaagccc tcctgctcgt gatggacggg ctcggcggct accggacccg
     2881 cgagcgcggc tccgagctca ccgaggccag cacccccaac ctcgaccggc tggccggcgt
     2941 gggctcgctc ggcctccacg accccgtcac ccgcggcatc acccccggct cgggcgccgg
     3001 ccacctcggc ctgttcggct acgacccgct cgcctacgag atcggccgcg gcgccctctc
     3061 ggccgccggg atcggcttcg agctccagcc cggcgacgtg gccgcccggg tcaacttctg
     3121 caccatcaac cccgacggga ccatcgccga ccgccgcgcc ggccggctgc ccaccgagga
     3181 ggccgccggc atctgcgacc tgctggtgcg cgagatccag ctcgacgggg tccagttctt
     3241 cctccagccc gagcgggagc accgcggcct gctggtcctg cgcggcccag gcctcgaccg
     3301 cgacgtgacc gacaccgatc cccagcgcga gggcgtgccc ccgctcgacc cggtggcccg
     3361 ctcgcccgag gccaagcgga ccgccgagct catcggccgg atcctcgacc aggcccggac
     3421 cctgctcgcc gaccgggagc gcggcaactt cctgctcctg cgcggcttcg acagcgtcaa
     3481 ggagatgccc agcttccccg accgctacca ggtccgggcc ctggccatcg ccgcctaccc
     3541 gatgtacgtc ggcatctccc ggctgctcgg cttccagacc caccaggtcg acggggccgc
     3601 ccccgaggag gtcgacgccc tggccgagct gctgcccgac cacgacttcg tgttcatgca
     3661 catcaagaag accgactcgg ccggcgagga cggcgacttc gagcgcaagg tccaggccat
     3721 cgaggaggtc gacgccctga tcccgcgcat gctcgacgcc ggggtcgacg tcctggccgt
     3781 caccggcgac cactccaccc cggcccagat ggccgcccac tcctggcacc cggtgcccgt
     3841 cctgctctgg ggcggcaccg ccgccgccga cggcctcccc gccttcgacg aggtcacctg
     3901 ccgccagggg tccctcggcc gcttcgaggc caagcacctc ctgccccagc tcctcgccgc
     3961 cgcagggcgg ctcaccaagt acggcgccta gacacagaga ggacccagcc cgtgaagggt
     4021 gtgatcctgg ccggcggtac cggctcccgg ctgcat
//
LOCUS       DASSWR010000198         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1307653, whole genome shotgun sequence.
ACCESSION   DASSWR010000198 DASSWR010000000
VERSION     DASSWR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307653"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFO47_03250"
     CDS             complement(<1..476)
                     /locus_tag="VFO47_03250"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8787744.1"
                     /translation="MPRLVKDSPLEVGGRTVKVSSVAKPFFPDAGLTKGDMIAYYRDV
                     AEVMLPHLAGRPLNLQRFPNGVNGKGFWQQGASDHFPGWVRTLTVERRGRGGTVDHVV
                     CDDAATIVYLANLATVTFHAWTSRVEDLARPELLIIDLDPDPDQELDVVLAAARAV"
     gene            complement(520..1035)
                     /locus_tag="VFO47_03255"
     CDS             complement(520..1035)
                     /locus_tag="VFO47_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8787745.1"
                     /translation="MRYDPFIWPEWAEVLQQLWELTSPEAAAASLQAVKARLGVAFQQ
                     CEPWPGWDKDHYDGPWNRTTANPVLVVGNLFDPATPYHGAVTVDRLLPRSRLLTLAGW
                     GHTSLFASACVDAHVSAYLLTTQVPSRGAVCEPDVVPFAEPAAAAQTLQAGGPSKAAV
                     IPPLLRRLLTD"
     gene            complement(1198..1566)
                     /locus_tag="VFO47_03260"
     CDS             complement(1198..1566)
                     /locus_tag="VFO47_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8787746.1"
                     /translation="MTRAEERIWVRSDRAVARACAERAGPILDHMSTANVARDLDLLR
                     RAVGDAKLTYAGYSYGSYLGSTYANLFPGKVRALVVDGVLDPVAWSTGRGDQARRLPF
                     STRLGSAKGAYETLQEFLRL"
     gene            complement(1563..2078)
                     /locus_tag="VFO47_03265"
     CDS             complement(1563..2078)
                     /locus_tag="VFO47_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787747.1"
                     /translation="MAQSSKARHRLRRGVLAALVAALAATLFTLPPSAGATTPPAASG
                     TGSAAVPQLDWGSCGEEPELQPFQCATAVVPLDHDKPKGKQITLALARLPAGDPSRKI
                     GSLFLNPGGPGGSGVDFLFGAGPFLYSDEVRARFDLVGFDPRGIIRSTPLRCYETFDE
                     AVADLAAFPSR"
BASE COUNT          313 a          761 c          877 g          291 t
ORIGIN      
        1 ttgaccgccc gggcggccgc caggaccacg tccagctcct gatccgggtc ggggtcgagg
       61 tcgatgatga gcagttcggg ccgggccagg tcctcgaccc tcgaggtcca ggcgtggaag
      121 gtcaccgtgg ccaggttggc caggtagacg atggtggcgg cgtcgtcgca gaccacgtgg
      181 tcgacggtcc cgccgcggcc ccggcgctcc acggtgaggg tccggaccca gcccgggaag
      241 tggtcggagg cgccctgctg ccagaacccc ttgccgttga ccccgttggg gaaccgctgg
      301 aggttgagcg gccggccggc caggtgcggg agcatgacct cggccacgtc gcggtagtag
      361 gcgatcatgt cgcccttggt caggccggcg tcggggaaga acggcttggc gacgctggac
      421 accttgacgg tccgcccgcc gacctccagc gggctgtcct tcacgaggcg aggcatgggc
      481 tcagcctacg ccccgtgaag gacagcgccg ccgggtcgtt cagtcggtca gcagcctccg
      541 cagcagcggc gggatcacgg ccgccttgct cggcccgccg gcctgcagag tctgggcggc
      601 agcggccggc tcggcgaacg gcaccacgtc cggctcgcac accgcgcccc gtgacgggac
      661 ctgcgtggtg agcaggtagg cgctcacgtg ggcgtcgacg caggccgagg cgaacagcga
      721 ggtgtgcccc cagccggcca gggtgagcag ccgcgaccgc ggcagcagcc ggtcgacggt
      781 gaccgccccg tggtaggggg tggccgggtc gaacaggttg ccgaccacca gcaccgggtt
      841 ggcggtggtg cggttccagg ggccgtcgta gtggtccttg tcccagcccg gccagggctc
      901 gcactgctgg aacgccaccc cgagccgggc cttgacggcc tgcaggctgg ccgcggccgc
      961 ctcgggactg gtgagctccc acagctgctg gaggacctcg gcccactcgg gccagatgaa
     1021 cgggtcgtag cgcacgccga gggtggtgcc gatcagctcg gcgtaggtga ccaggaacgt
     1081 ctcgccgctc gcggggtcgg tgaactcgac cggctcgcgc agcagccgcc tggccagccg
     1141 gtcgcagcgg cggacggggt tgccttcgga gaaggtgcgg ttggggccgc cggctcctca
     1201 cagccgcagg aactcctgca gggtctcgta cgcgcccttg gcgctgccca gcctcgtcga
     1261 gaacggcagc ctgcgggcct ggtcgccgcg cccggtggac caggcgaccg ggtcgaggac
     1321 accgtcgacg accagggccc ggaccttgcc cgggaacagg ttggcgtagg tcgagcccag
     1381 gtaggagccg taggagtagc cggcgtaggt cagcttggcg tcgccgacgg cccggcgcag
     1441 caggtccagg tcgcgggcca cgttggcggt cgacatgtgg tccaggatcg gcccggcgcg
     1501 ctcggcgcag gcccgggcga cggcccggtc agagcggacc cagatccgct cctccgccct
     1561 ggtcaccggg acgggaacgc ggccaggtcg gccacggcct cgtcgaaggt ctcgtagcag
     1621 cgcagcgggg tgctgcggat gatgccccgg gggtcgaagc cgaccaggtc gaagcgggcc
     1681 cggacctcgt cggagtagag gaacgggccg gcgccgaaca ggaagtcgac gcccgagccg
     1741 cccggcccgc ccgggttgag gaacagcgag ccgatcttgc ggctggggtc gccggccggg
     1801 agccgggcca gggcgagggt gatctgcttg cccttgggct tgtcatggtc cagcggcacc
     1861 acggcggtgg cgcactggaa gggctggagc tcgggctctt cgccgcagct gccccagtcc
     1921 agctggggca cggccgccga gccggtgccc gacgcggccg ggggcgtcgt ggcgccggcc
     1981 gagggcggga gggtgaacag ggttgccgcc agcgcggcca ccagggccgc gagcacgccc
     2041 cggcggagcc ggtgccgcgc cttcgaggac tgtgccaaga cgagaccccc ctcgggcatg
     2101 caccggcgac cccggacggt accggcatcg gccggatgtt acctctgtag gcatccaggc
     2161 cgaaacgggc cgaactgggg attgaccgaa accgggcggt ctggcagagt ccggatcgac
     2221 ggcacggtgg ccctttgatt gt
//
LOCUS       DASSWR010000199         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1223575, whole genome shotgun sequence.
ACCESSION   DASSWR010000199 DASSWR010000000
VERSION     DASSWR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223575"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /gene="sufD"
                     /locus_tag="VFO47_03270"
     CDS             <1..1331
                     /gene="sufD"
                     /locus_tag="VFO47_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET8787748.1"
                     /translation="DWFLARRLEALAALAKLERPTNREEAWRFTDPKRAGLDRARLLR
                     TGTGSGVEGAHVLAAGHAHAASGGDVAGTELEPAGLVATVDGATAEAFLAPDLAAKGV
                     LLTDLATAVREHAGLVEPRFMTSAAPFDEDWFLALHATLVTAGAFLYVPRGVELTVPI
                     GVLQRRTTGGATFAHTLAVVEPEASLTLVQQHGSPETLDGRAFHHGVTELLIGERASV
                     QYLSLQEWGSEQVNHFGVQRAVAGRQARFKSFVVTLGGGVVRVSPEVVLGEGAEADLL
                     GAVFADAGQRFEHRANVTHAEPHARSSLLYKAGLLGGSRNIFNGNLIISPGARGTDAG
                     QTMRNLVLSRDAHAEANPFLEILNSDVKAAHAAATGRVDDLHLFYLESRGIPRRTARR
                     LVVFGVFEEVLAQVTVPEIRRRLEAALEAELAAAAAAQEAQEPAQAREAG"
     gene            1331..1675
                     /locus_tag="VFO47_03275"
     CDS             1331..1675
                     /locus_tag="VFO47_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET8787749.1"
                     /translation="MPFVRLAGLSELEPDQGLRVELGDEEAVALIRTADGGVHAVIDV
                     CSHEDYPLSEGWVEDTTIECALHGSRFDLLTGEPDSPPAVRPVRIFPVRVEGEDVLVD
                     LPDDWAKLANDL"
     gene            1694..2446
                     /gene="sufC"
                     /locus_tag="VFO47_03280"
     CDS             1694..2446
                     /gene="sufC"
                     /locus_tag="VFO47_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET8787750.1"
                     /translation="MPDTTLVIEVLHVDVEDKQILRGVDLTVESGKVHALMGPNGSGK
                     STLAYALAGHPRYRVTGGKVTFKGEDVLDLTPDERARLGMFLAMQYPVEVPGVSVTNF
                     LRTAMNSVRGEDVPVREFAKDLREQMRLLDMDPRFAERSLNEGFSGGEKKRHEILQMA
                     LLRPELAILDETDSGLDVDALRVVADGVNRLRGPQLGVLLITHYTRILRYISPDMVHV
                     MVDGRVVESGGPELADQLEAEGYERWRGDPAT"
     gene            2469..3707
                     /locus_tag="VFO47_03285"
     CDS             2469..3707
                     /locus_tag="VFO47_03285"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET8787751.1"
                     /translation="MTSETAPLDVAAVRGDFPILERRVNGHALVYLDSAATSQKPAQV
                     LEAMDTYYRRHNANVHRGVHTLAEEATHLFEEARGKVARFVGASERATVFTKNVSEAI
                     NLVAYAWGLRNLRQGDEILVTEMEHHSNLVPWQLVAGMTGARVRAVPVTGDYLLDLDA
                     LDGLLGERTRIVAVSAMSNVLGTVNPVAEIAERAHAAGALVLADAAQAVPHAGVRLDT
                     LGVDFLGFTGHKALGPMGVGVLAAREELLESMEPFLGGGEMIRDVDIDRATWNDIPWR
                     FEAGTPNVAEAVGLGAAVDYLQGLGMDRVRAHEVDVTGYALKRLGEIDGLRIYGPSEV
                     EHRGGMVSFTLGDLHPHDVAQVLDSDGIAVRAGHHCAKPLLRRLGVQATTRASFYLYS
                     TTEEVDALVAGLDKVRRFFA"
     gene            3725..4171
                     /locus_tag="VFO47_03290"
     CDS             3725..4171
                     /locus_tag="VFO47_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET8787752.1"
                     /translation="MPLDDLYQEIILDHYKKPRHPGRLEDAQVEVHHYNPVCGDELTV
                     GLRLDGDRVAAVAVDGKGCSISQAAASVMTEQVTGRPLGEALAQAEAFRRLMHGEGEP
                     DEELLGDGIAFKGVARYPVRVKCALLGWMAFKDAAARAGTEEEGRA"
     gene            4172..4489
                     /locus_tag="VFO47_03295"
     CDS             4172..4489
                     /locus_tag="VFO47_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET8787753.1"
                     /translation="MATDSDILEAMKQVEDPELGVNVVDLGLVYGVDQEEEGRKVRLD
                     MTLTTVACPLTDMIEGATNAALIGLDDIQEVEINWVWSPPWGPERLTEDGKVALRAMG
                     FAI"
     gene            4528..>5465
                     /gene="moeB"
                     /locus_tag="VFO47_03300"
     CDS             4528..>5465
                     /gene="moeB"
                     /locus_tag="VFO47_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007444460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET8787754.1"
                     /translation="MALPALVEPAAELSRDEIARYSRHLIIPDVAMDGQKRLKNARVL
                     LIGAGGLGAPNAMYLAAAGVGHLGLVDFDVVDASNLQRQVIHGTSDIGRPKLESARDK
                     ITEINPYVQVTLHETRLSSENARELIRGYDLVVDGSDNFPTRYLVNDACVLEGKPYVW
                     GSIYRFDGQASVFWAEHGPCYRCLYPEPPPPGMVPSCAEGGVLGVLCATVGSVQANEA
                     VKLLTGIGDPLVGRLLIYDALELSFKDISVRKDPECAVCGKNPTVTELIDYEEFCGVV
                     SDEAQQAAAGATLTPAQLKALLDDGRELKLVDVREPA"
BASE COUNT          804 a         2021 c         1917 g          723 t
ORIGIN      
        1 ccgactggtt ccttgcccgg cgcctggagg cgctggccgc cctggccaag ctggagcggc
       61 cgaccaaccg tgaggaggcc tggcggttca ccgaccccaa gcgggccggc ctggaccgcg
      121 cccggctcct caggaccggc accggcagcg gcgtcgaggg cgcccacgtg ctggcggccg
      181 gccacgcgca cgccgcctcc ggcggtgacg tcgccggcac cgagctcgag ccggccgggc
      241 tggtcgccac cgtcgacggc gccaccgccg aggcgttcct cgcccccgac ctggccgcca
      301 agggcgtgct cctgaccgac ctggccacgg ccgtgcgtga gcacgccggc ctggtcgagc
      361 ccaggttcat gacctcggcg gccccgttcg acgaggactg gttcctggcc ctgcacgcca
      421 ccctggtcac cgccggggcg ttcctgtacg taccccgcgg ggtcgagctg accgtcccca
      481 tcggggtgct gcagcggcgc accaccggcg gggcgacctt cgcccacacc ctggccgtgg
      541 tcgagcccga ggcgtccctc accctggtcc agcagcacgg ctcgcccgag accctcgacg
      601 ggcgcgcctt ccaccacggc gtcaccgagc tgctgatcgg cgagcgggcc agcgtccagt
      661 acctgtcgct ccaggagtgg gggagcgagc aggtcaacca cttcggcgtc cagcgggccg
      721 tggccgggcg ccaggcccgg ttcaagtcct tcgtggtcac cctggggggc ggggtggtcc
      781 gggtgtcgcc cgaggtcgtg ctgggcgagg gcgccgaggc cgacctgctc ggggccgtgt
      841 tcgccgacgc cggccagcgc ttcgagcacc gggccaacgt cacccacgcc gagccccacg
      901 cccgctccag cctcctctac aaggccgggc tgctgggcgg gtcgcgcaac atcttcaacg
      961 gcaacctgat catcagcccc ggggcccgcg gcaccgacgc cggccagacc atgcgcaacc
     1021 tggtcctgtc ccgcgacgcc cacgccgagg ccaacccgtt cctggagatc ctcaactccg
     1081 acgtcaaggc ggcccacgcg gccgccaccg gccgggtcga cgacctgcac ctgttctacc
     1141 tggagtcgcg gggcatcccc cggcggacgg cccggcggct ggtcgtgttc ggggtcttcg
     1201 aggaggtcct ggcccaggtg accgtgcccg agatccggcg ccgcctggag gccgccctgg
     1261 aggccgagct ggccgcagcc gcggccgccc aggaggccca ggagccggct caggcccggg
     1321 aggccgggtg atgccgttcg tgcgcctggc cgggctgtcg gagctggaac ccgaccaggg
     1381 gctccgggtc gagctcggcg acgaggaggc ggtcgccctg atccgcaccg ccgacggcgg
     1441 tgtccacgcc gtgatcgacg tgtgcagcca cgaggactac ccgctgtccg agggctgggt
     1501 cgaggacacc accatcgagt gcgccctgca cggcagccgc ttcgacctgc tcaccggcga
     1561 gcccgacagc ccgccggcgg tgcgcccggt gcggatcttc ccggtgcggg tcgagggcga
     1621 ggacgtgctc gtcgacctgc ccgacgactg ggccaagctg gccaacgacc tgtgatgcat
     1681 cccgaggaga accatgcctg acacgaccct ggtgatcgag gtcctccacg tcgacgtgga
     1741 ggacaagcag atcctgcgcg gcgtcgacct gaccgtggag tcggggaagg tccacgccct
     1801 gatgggcccc aacggctccg gcaagtcgac cctggcctac gccctggccg gccacccccg
     1861 ctaccgggtg accggcggca aggtgacgtt caagggcgag gacgtgctcg acctcacccc
     1921 tgacgagcgg gcccggctgg ggatgttcct ggccatgcag tacccggtcg aggtgccggg
     1981 ggtgtcggtg accaacttcc tgcgcacggc catgaactcg gtccgcggcg aggacgtccc
     2041 cgtgcgcgag ttcgccaagg acctgcgcga gcagatgcgc ctgctcgaca tggacccgcg
     2101 gttcgccgag cgcagcctca acgagggctt ctcgggcggc gagaagaagc gccacgagat
     2161 cctccagatg gccctgctcc gccccgagct ggccatcctc gacgagaccg actcgggcct
     2221 ggacgtggac gccctgcggg tcgtggccga cggcgtcaac cgcctccgcg gcccccagct
     2281 cggtgtcctg ctgatcaccc actacacccg catcctgcgc tacatctccc ccgacatggt
     2341 ccacgtgatg gtcgacggcc gggtcgtgga gtccggcggt cccgagctgg ccgaccagct
     2401 ggaggccgag ggctacgagc gctggcgtgg cgaccccgcc acctgacacc cggtccagga
     2461 ggcccccgat gacgtctgag accgcgccgc tggacgtggc cgccgtccgc ggcgacttcc
     2521 cgatcctgga gcgccgggtc aacggccacg ccctcgtcta tctggactcg gccgccacca
     2581 gccagaagcc ggcgcaggtg cttgaggcca tggacaccta ctaccggcgc cacaacgcca
     2641 acgtgcaccg gggcgtccac accctggccg aggaggccac ccacctgttc gaggaggccc
     2701 ggggcaaggt ggcgcgcttc gtcggcgcct ccgagcgggc cacggtgttc accaagaacg
     2761 tcagcgaggc catcaacctg gtcgcctacg cctggggcct gcgcaacctc cgccagggcg
     2821 acgagatcct ggtcaccgag atggagcacc actccaacct ggtgccctgg cagctggtgg
     2881 ccggcatgac cggggccagg gtccgggccg tgcccgtcac cggtgactac ctgctcgacc
     2941 tggacgccct tgacggcctg ctcggcgagc ggacccgcat cgtcgccgtg tcggccatgt
     3001 cgaacgtgct cggcaccgtc aacccggtgg ccgagatcgc cgagcgggcc cacgccgccg
     3061 gggcgctcgt cctggccgac gccgcccagg ccgtgcccca cgccggggtc cgcctcgaca
     3121 ccctcggggt cgacttcctc gggttcaccg ggcacaaggc cctcggcccc atgggggtcg
     3181 gggtgctggc cgcccgggaa gagctgctgg agagcatgga gccgttcctc ggcgggggcg
     3241 agatgatccg cgacgtcgac atcgaccggg ccacctggaa cgacatcccg tggcggttcg
     3301 aggccggtac gcccaacgtg gccgaggcgg tcgggctggg agcggccgtc gactacctcc
     3361 aggggctcgg catggaccgg gtccgggccc acgaggtcga cgtcaccggc tacgccctca
     3421 agcgcctggg cgagatcgac gggctccgga tctacgggcc gtccgaggtc gagcaccgcg
     3481 gcggcatggt ctcgttcacc ctcggcgacc tccaccccca cgacgtcgcc caggtgctcg
     3541 actccgacgg gatcgcggtc cgggccggcc accactgcgc caagccgctg ctgcggcgcc
     3601 tcggggtcca ggccaccacc cgggcgtcgt tctacctcta ctcgaccacc gaggaggtcg
     3661 acgcgctggt cgccggcctc gacaaggtca ggcgcttctt cgcctagcag gcaaggacac
     3721 cgagatgccc ctcgacgacc tctaccagga gatcatcctc gaccactaca agaagccccg
     3781 gcacccgggg cggctcgagg acgcccaggt ggaggtgcac cactacaacc cggtgtgcgg
     3841 ggacgagctg acggtcgggc tgcggctgga cggcgaccgg gtcgcggccg tggccgtcga
     3901 cggcaagggc tgctcgatct cccaggcggc cgcctcggtc atgaccgagc aggtgaccgg
     3961 ccggcccctg ggcgaggccc tcgcccaggc cgaggcgttc cggcggctga tgcacggcga
     4021 gggcgagccg gacgaggaat tgctggggga cgggatcgcg ttcaagggag tggcgcgcta
     4081 ccccgtgcgg gtcaagtgcg cgctgctcgg ctggatggcg ttcaaggacg ccgccgcccg
     4141 ggcagggaca gaagaggaag gaagagcgta gatggccacc gatagcgaca tcctcgaggc
     4201 gatgaagcag gtcgaggacc ccgagctggg cgtcaacgtg gtcgacctgg gcctggtgta
     4261 cggcgtggac caggaggagg agggccgcaa ggtgcggctc gacatgaccc tgaccacggt
     4321 ggcctgcccg ctcaccgaca tgatcgaggg tgcgaccaac gccgccctga tcggcctgga
     4381 cgacatccag gaggtcgaga tcaactgggt ctggtcgccg ccatgggggc cggagcggct
     4441 caccgaggac ggcaaggtgg ccctgcgggc catgggcttc gcgatctgac gcggacgaga
     4501 gcgaacgacg agggatctgg acagaagatg gcgcttcctg cactggttga accggcggcc
     4561 gagctgtcgc gggacgagat cgcccgctac tcccgccacc tgatcatccc cgacgtggcc
     4621 atggacgggc agaagcggct caagaacgcc cgcgtcctgc tcatcggcgc cggcggcctc
     4681 ggcgccccca acgccatgta cctggccgcg gccggggtcg ggcacctcgg cctggtcgac
     4741 ttcgacgtgg tcgacgcctc caacctccag cggcaggtca tccacggcac ctccgacatc
     4801 ggccggccca agctggagag cgcccgcgac aagatcaccg agatcaaccc gtacgtgcag
     4861 gtcaccctgc acgagacccg gctctcctcg gagaacgccc gcgagctgat ccgcggctac
     4921 gacctggtgg tcgacggctc cgacaacttc cccacccgct acctggtcaa cgacgcctgc
     4981 gtgctcgagg gcaagccgta cgtgtggggc tcgatctacc gcttcgacgg ccaggccagc
     5041 gtgttctggg ccgagcacgg cccctgctac cgctgcctgt accccgagcc gcccccgccg
     5101 ggcatggtcc cctcctgcgc cgagggcggc gtgctgggcg tgctctgcgc caccgtcggc
     5161 tcggtccagg ccaacgaggc cgtcaagctg ctcaccggca tcggcgaccc gctggtcggg
     5221 cggctgctga tctacgacgc cctggagctg tccttcaagg acatcagcgt ccgcaaggac
     5281 cccgagtgcg ccgtctgcgg caagaacccc acggtcaccg agctgatcga ctacgaggag
     5341 ttctgcggcg tcgtctccga cgaggcccag caggccgccg ccggcgccac cctgaccccg
     5401 gcccagctca aggcgctgct cgacgacggt cgggagctca agctggtgga cgtccgcgag
     5461 ccggc
//
LOCUS       DASSWR010000200         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1194287, whole genome shotgun sequence.
ACCESSION   DASSWR010000200 DASSWR010000000
VERSION     DASSWR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194287"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFO47_03305"
     CDS             complement(<1..277)
                     /locus_tag="VFO47_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8787755.1"
                     /translation="MTEVWAAGDAYESYVGRWSRAVAAEFVGWLGLAEGGAWLDVGCG
                     TGALSQAVLSAADPALVVGVDPSPGFLAQARTRLGAHARLAAADARWL"
     gene            complement(328..963)
                     /locus_tag="VFO47_03310"
     CDS             complement(328..963)
                     /locus_tag="VFO47_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787756.1"
                     /translation="MMQLDAPGRDVPADPATLRLPPAALLVVAGVPGAGKTTLLSRIQ
                     APGSLILDPEPIRARYQRRLGRVPYRLWRPLVHAEHALRVLAALPGPFGLIVHDTGTR
                     GWRRRGLVGLARRCGRSGHLLLLDVSAEAALEGQRRRRRALHGAAFAAHWRNWRRLRA
                     ELFVPVDPSGPQLPGDPGAEASGLRTEGWASVRLLDRPSANRLGRIDIPPR"
     gene            1018..1731
                     /locus_tag="VFO47_03315"
     CDS             1018..1731
                     /locus_tag="VFO47_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787757.1"
                     /translation="MPGRRSRAAVALLVAGFLAGCGGSGTALVDSSTTRPVPTTTVAT
                     STTRPGTTAAPTTRAIPTTTGRVPLTTGQSGPAGAPLTFRHLTIRLAEGWRAGGGGDH
                     VEVATGRACRRSAGGVDCPGFLLLGPSQIAIASQLAPYDPDKVWHPGAGVEGCPQDRD
                     GLAEEPPAKPSKKGVAKVGGKDAVYREWRIGCVDATTGEPETGYTQRIWYLKASKILV
                     VDEWSTPGLKEALAAGRFG"
     gene            complement(1742..>2535)
                     /locus_tag="VFO47_03320"
     CDS             complement(1742..>2535)
                     /locus_tag="VFO47_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8787758.1"
                     /translation="LVARVLSRLGLVHAFGHVSARASAGPVGGSAGPAGAVDSREHLV
                     SGSATLPLRGASQPEAGVPPAPTRQAGPPPVASWTVLLTPTSPPLGEVAAGDVLELDQ
                     GGRVLRGAAGVVPLEAPLHLGVYAARSDVGAVCRLHGPAVAAFAATGAGPPLLHGFGG
                     MVEPVAFWDDPDLVTGEPGAAGVAAVLGTAPAMLLAGNGGLTVGADLDQALARAWCLE
                     DRCRVALAVGGRGRPLAPEALARRRHWYEAETARLARWLRHTAGP"
BASE COUNT          319 a          992 c         1002 g          222 t
ORIGIN      
        1 gcagccaccg ggcgtcggcg gccgcgagcc gggcatgtgc gcccagccgc gtccgcgcct
       61 gggcgaggaa gcccggcgac gggtcgacgc cgacgaccag ggccgggtcg gccgccgaca
      121 gcacggcctg gctcagggcg ccggtgccgc agccgacgtc cagccaggcg ccgccttccg
      181 ccagccccag ccagccgacg aactcggcgg ccacggcccg gctccagcgg ccgacgtacg
      241 actcgtaggc gtcaccggcc gcccagacct cggtcatggc gggaagtctc cccgatcggc
      301 cgtgcctgcg acacccggcg gggtcggtca gcgcggcggg atgtcgatcc ggcccagccg
      361 gttggcggag gggcggtcca gcaggcggac cgaggcccac ccctcggtcc ggagcccgga
      421 cgcctcggcg cccgggtcgc ccgggagctg ggggccggac gggtcgacgg ggacgaacag
      481 ctcggcccgg agccgccgcc agttgcgcca gtgggcggcg aaggccgcgc cgtgcagggc
      541 ccggcggcgg cggcgctggc cctccagggc ggcctcggcg gagacgtcga gcaggagcag
      601 gtggccgctg cggccgcagc gccgggccag gccgaccagg ccccgccggc gccagccccg
      661 ggtgccggtg tcgtggacga tcaggccgaa cgggccgggc agagcggcca ggacccgcag
      721 ggcgtgctcg gcgtggacca gcggccgcca cagccggtag gggacccgcc cgagccggcg
      781 ctggtacctg gcccggatcg gctccgggtc gaggatcagg gaccctgggg cctggatgcg
      841 cgacagcagg gtcgtcttgc cggcgccggg cacgcccgcc accaccagca gggccgccgg
      901 gggcaggcgc agcgtcgccg ggtccgccgg gacgtcgcgc ccgggcgcgt cgagctgcat
      961 catggcggcg acgatactgg tcccggcgga aagccggtgt aaaggtcgcg aggtgtgatg
     1021 ccgggacggc gctccagggc ggcggtcgcg ctgctggttg cggggttcct ggccggctgc
     1081 ggcgggtcgg ggaccgccct ggtcgactcc tcgacgaccc ggccggtccc gaccaccacc
     1141 gtggccacct cgacgacccg gcccgggacc acggccgcgc ccacgacccg ggcgatcccg
     1201 accaccacgg gccgggtccc gctgaccacc gggcagtccg gcccggccgg ggcgccgctg
     1261 accttccggc acctcaccat ccggctcgcc gagggctggc gggccggcgg cggcggcgac
     1321 cacgtggagg tcgcgaccgg ccgggcctgc cggcgcagtg ccggcggggt cgactgtccc
     1381 gggttcctgc tcctcggccc cagccagatc gccatcgcca gccagctcgc cccctacgac
     1441 ccggacaagg tctggcaccc aggggccggg gtcgagggct gcccccagga ccgcgacgga
     1501 ctggccgagg agccgccggc caagccgtcc aagaagggcg tggccaaggt cggcggcaag
     1561 gacgccgtct accgcgagtg gcgcatcggc tgcgtcgacg ccaccaccgg cgaacccgag
     1621 accggctaca cccagcggat ctggtacctg aaggcgtcca agatcctggt cgtcgacgaa
     1681 tggtcgaccc ccggcctcaa ggaggccctg gccgccggca ggttcggctg acccgggccc
     1741 gtcaggggcc ggcggtgtgg cgcagccagc gggccaggcg ggccgtctcc gcctcgtacc
     1801 agtggcggcg gcgggccagc gcctcggggg cgagcgggcg gccccggccg ccgacggcca
     1861 gggcgacccg gcagcggtcc tccaggcacc aggcgcgggc cagggcctgg tcgaggtcgg
     1921 ccccgacggt caggccgccg ttgccggcca gcagcatggc cggggcggtc ccgagaacgg
     1981 ccgcgacccc ggccgcgccc ggctccccgg tcaccaggtc ggggtcgtcc cagaaggcga
     2041 ccggctccac catccccccg aacccgtgca gcagcggcgg ccccgccccg gtggcggcga
     2101 acgcggccac cgccggcccg tgcagccggc agaccgcgcc cacgtcggac cgggcggcgt
     2161 agacgccgag gtgcagcggc gcctccaggg gcacgacccc ggccgccccc cgcagcaccc
     2221 gcccgccctg gtcgagctcg agcacgtcgc ccgccgccac ctcccccaac ggcggagagg
     2281 tcggggtgag cagcacggtc cagctggcga ccggcggagg cccggcctgg cgggtcgggg
     2341 caggcggcac cccggcctcc ggctgcgagg ccccccggag ggggagcgtg gccgacccgg
     2401 aaacgaggtg ctcgcggctg tccacagccc ccgccggccc ggcggaaccc cccaccggac
     2461 ccgcggaggc cctggcgctg acgtggccga aggcgtggac caggccgagg cgggacagga
     2521 cccgggcgac caggg
//
LOCUS       DASSWR010000201         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1279702, whole genome shotgun sequence.
ACCESSION   DASSWR010000201 DASSWR010000000
VERSION     DASSWR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279702"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFO47_03325"
     CDS             <1..206
                     /locus_tag="VFO47_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HET8787759.1"
                     /translation="VPQVMRLTEACESSGSFGLVETSEAIFRPWHVDGLAVRPEHRMV
                     GHTGFLVSARRVDGPWPSPGRLP"
     gene            261..437
                     /locus_tag="VFO47_03330"
     CDS             261..437
                     /locus_tag="VFO47_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET8787760.1"
                     /translation="MATDDKLRNQGEELKGKAKESLGRATDDEELEAAGRTDQTKANL
                     KQTGEKVKDAIKDA"
     gene            691..2421
                     /gene="arc"
                     /locus_tag="VFO47_03335"
     CDS             691..2421
                     /gene="arc"
                     /locus_tag="VFO47_03335"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HET8787761.1"
                     /translation="MPEEAPEPSELQRQIQFLEEETALLRRRLQDSPRQVRVLEERLL
                     ETKGQLAQALSQNERLAATLREAREQIIALKEEVEKLTAPPSGFGSFVSVNEDGTINI
                     TSGGRKLRVNVHPDIDPKSLQHGQELMLNEALNVVEACAFEVQGEVVQLKEMLGVDRA
                     LVIGNADEERVVQIGEPLRNRTLRAGDSLLLDVRSGFVLEHLPKPEVEELILEEVPDI
                     SYQDIGGLADQIEQIRDAVELPFLHADLFAEHQLKPPKGILLYGPPGCGKTLIAKAVA
                     NSLAKKVAEVTGQPSGRSYFLNIKGPELLNKYVGETERQIRLIFQRAKEKSNEGVPVI
                     VFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVEQLKNVIVIGASNREDMIDPAI
                     LRPGRLDVKIKIERPDASAAKDIFSKYLLASLPLAEDVVEVAGGDREAALARIIDEGV
                     KRMYSDADENRFLEVTYANGDKEVLYFKDFNSGAMIENIVNRAKKMAIKRFLETGEKG
                     IKAADLFGAVTDEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLIEGGNSKDLGRT
                     IEQVNAGQYL"
     gene            2459..>2676
                     /locus_tag="VFO47_03340"
     CDS             2459..>2676
                     /locus_tag="VFO47_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET8787762.1"
                     /translation="MAITKVMGIETEYGITVPGAPDSNPVLASSLLVNAFSGRGRKVR
                     WDYEEESPLRDARGFEAAREPETPAEDDL"
BASE COUNT          484 a          939 c          901 g          352 t
ORIGIN      
        1 ccgtgcccca ggtgatgcgc ctgaccgagg cgtgcgagtc ctcgggcagc ttcggcctgg
       61 tcgagacctc ggaggccatc ttccgcccct ggcacgtcga cggcctggcc gtccggcccg
      121 agcaccgcat ggtcggccac accggcttcc tggtctcggc ccgccgggtc gacggcccct
      181 ggccgtcccc cggccgcctc ccctgaccgg tttgcgcccg ggcgtcaggg ggcaggcagc
      241 gccgcggagg tgatgcccac gtggcgaccg acgacaagct tcgcaaccag ggcgaggagc
      301 tgaagggcaa ggccaaggag tccctcggcc gcgccaccga cgacgaggag ctggaggcgg
      361 ccggccggac cgaccagacc aaggccaacc tcaagcagac cggcgagaag gtgaaggacg
      421 ccatcaagga cgcctgagcc gctgccgacc atccaccggg ccgccgcctc cgggcgcggc
      481 ccggtcgtcg tatccggggg agggccggta gcatggcggc tgcggaccac tcccagattt
      541 cgtcacaagc gcgctattcg aagagccgat agggtttagg caagtctacc tagccatcca
      601 tcggtagcct ccaccaccgc cgaagggggg actcacgtga aggacgccga ccgctcccgg
      661 cagtctcggt cccgtcgcga ccccgacccc atgccagagg aggcgcctga gcccagcgag
      721 ctgcaacgcc agatccagtt cctcgaggag gagacggcct tgctccgccg ccgcctgcag
      781 gactcgcccc ggcaggtccg ggtgctcgag gaacgcctcc tcgagaccaa gggccagctg
      841 gcccaggccc tgtcccagaa cgaacgcctg gccgccaccc tgcgcgaggc cagggagcag
      901 atcatcgccc tcaaggagga ggtcgagaag ctcaccgccc cgccctcggg cttcgggtcg
      961 ttcgtcagcg tcaacgagga cggcaccatc aacatcacct cgggcggccg caaactgcgg
     1021 gtcaacgtcc accccgacat cgaccccaag tcgctccagc acggccagga gctgatgctc
     1081 aacgaggccc tgaacgtggt cgaggcctgc gccttcgagg tccagggcga ggtcgtgcag
     1141 ctcaaggaga tgctcggggt cgaccgggcc ctggtcatcg gcaacgccga cgaggagcgg
     1201 gtggtgcaga tcggcgagcc gctgcgcaac cgcaccctgc gggccggcga ctcgctgctg
     1261 ctggacgtcc gctccgggtt cgtgctcgag cacctgccca agccggaggt cgaggagctc
     1321 atcctggagg aggtgcccga catctcctac caggacatcg gcggcctggc cgaccagatc
     1381 gagcagatcc gcgacgccgt cgagctgccg ttcctgcacg ccgacctgtt cgccgagcac
     1441 cagctcaagc cgcccaaggg catcctgctc tacgggcccc ctggctgtgg caagaccctg
     1501 atcgccaagg cggtggccaa ctcgctggcc aagaaggtgg ccgaggtgac gggccagccc
     1561 tcggggcgca gctacttcct caacatcaag ggtcccgagc tgctcaacaa gtacgtgggg
     1621 gagaccgagc gccagatccg gctgatcttc cagcgggcca aggagaagtc caacgagggc
     1681 gtgccggtga tcgtgttctt cgacgagatg gactcgatct tccgcacccg cgggtcgggg
     1741 gtgtcctccg acgtcgagaa caccatcgtg ccccagctgc tgagcgagat cgacggggtc
     1801 gagcagctca agaacgtgat cgtgatcggc gcctccaacc gcgaggacat gatcgacccg
     1861 gcgatcctgc ggcccggccg gctcgacgtg aagatcaaga tcgagcggcc cgacgcctcc
     1921 gcggccaagg acatcttctc caagtacctg ctggcctccc tgcccctggc cgaggacgtg
     1981 gtcgaggtgg ccggcggcga ccgcgaggcc gccctggccc ggatcatcga cgagggcgtc
     2041 aagcggatgt actccgacgc cgacgagaac cgcttcctgg aggtcaccta cgccaacggc
     2101 gacaaggagg tcctgtactt caaggacttc aactccgggg cgatgatcga gaacatcgtc
     2161 aaccgggcca agaagatggc catcaagcgg ttcctggaga ccggtgagaa gggcatcaag
     2221 gcggccgacc tgttcggtgc cgtgaccgac gagttcaagg agaacgagga cctgcccaac
     2281 accaccaacc ccgacgactg ggcgcgcatc tcgggcaaga agggtgagcg catcgtgtac
     2341 gtgcggaccc tgatcgaggg cggcaactcc aaggacctcg ggcgcaccat cgagcaggtc
     2401 aacgccgggc agtacctcta ggcttcggcc caggggctgc cggggagggg aaccagggat
     2461 ggccatcacc aaggtcatgg gcatcgagac cgagtacggc atcaccgtgc ccggcgcgcc
     2521 cgactccaac ccggtgctgg cctccagcct gctcgtcaac gccttctcgg gccggggccg
     2581 caaggtccgc tgggactacg aggaggagtc gccgctgcgc gacgcccgcg ggttcgaggc
     2641 ggcccgcgag cccgagaccc cggccgagga cgacct
//
LOCUS       DASSWR010000202         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_968992,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000202 DASSWR010000000
VERSION     DASSWR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968992"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFO47_03345"
     CDS             <1..669
                     /locus_tag="VFO47_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8787763.1"
                     /translation="AVEGAGAVVGGQSAQSLDLARASARDRNVVIPLVLLVVLVILGV
                     LLRAVVAPLVLIATVIVSFAAALGASAFAFDRLLGFGGADPSLALLAFIFLVALGIDY
                     NIFLMSRVREESERLGTREGTLRGLAVTGGVITSAGIVLAATFSVLAVLPFVPLIEIG
                     IVVAFGVLLDTLVVRSILVPALTLDIGPRIWWPSRLAAARQPSADEVLDDSSIRARSR
                     SSSD"
     gene            complement(600..1139)
                     /locus_tag="VFO47_03350"
     CDS             complement(600..1139)
                     /locus_tag="VFO47_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET8787764.1"
                     /translation="MRVRPGRDRRAWSLGAPRQPARLQLQPHYDPEAFGRFSEAIARF
                     LGTGRYLVFQTAVVSLWVAWNVLFRNREAAFDPYPFILLTLALSLQASYAAPLILLAQ
                     NRQAERDRQETERDRRTSGRTQADTEYLARELAAIRLALGEMPTRDYLDDELERLQSE
                     LERLRARIEESSRTSSAEG"
     gene            complement(1136..2377)
                     /locus_tag="VFO47_03355"
     CDS             complement(1136..2377)
                     /locus_tag="VFO47_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018544343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8787765.1"
                     /translation="MAAGSEQLFVSRLARLPVLAPTGETIGRIADVVIARRESGAPPP
                     VLGFTVMVQRRPIFLGVGRVRELDESGATLSTGSVNLRPFEQREGETLVVGELLDARV
                     RHRETGRQLRLNDVAIGRVREEWVVTALDVLEPGGGLRPWRRAKHLRLPWEQVAGLEP
                     AETAERRVAALANLRPADLAAALQALPVHSRQEVVAGLDDDRLAEAMEELPEELQAVL
                     LAGLGDERAADVLEAMDPDDAADLLGELSEADRARLLAMMVPDEAEPVRRLLVYDEET
                     AGGLMTTEPVIMRAADTVAEALARVRDPDLPPALAAQVFVVRPPTQTPTGRYLGVAYL
                     QRLLREPPSEELGSCVDTDLDCIAPEEPTRRVAEYLATYDLLAAPVCDPQGRLLGAVS
                     IDDVLDHLLPEAWRRGAGAVR"
     gene            complement(2381..>5366)
                     /gene="metH"
                     /locus_tag="VFO47_03360"
     CDS             complement(2381..>5366)
                     /gene="metH"
                     /locus_tag="VFO47_03360"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET8787766.1"
                     /translation="ETCQDLLQVKVAVAGAFAGMRRANKKVPLITQVTVETTGQMLLG
                     SDIAAALTAIEPLGVDVIGMNCATGPAEMGEHLRYLAQHARTPLSCLPNAGLPELRDG
                     KPWYPLTPEELAEAHTRFAAEFGLGIVGGCCGTTPEHIRQVVEALGGRAPVPREPLHE
                     PSLSSLYQSVPLDQDTTYLTVGERCNANGSRKFRDLMLEGDLDGMVGIAKEQIREGAH
                     VLDVCVDYVGRDGVPDMDVLTARLAQQSTVPIMLDSTQADVLEAGLKRLGGRAIINSI
                     NLEDGEARMDAVCPMARQYGAALVALLIDEEGQARDVDWKLRIAHRIHDLAVDKHGLA
                     AEDLVFDALTFPLGSGQEDLRGDAVATLEAIRRIKAELPGVRTILGVSNVSFGLAPAA
                     RQVLNSVFLHEAREAGLDSAIVAPARILPMHRIDDEQRQVALDLIYDRRREGYDPLQK
                     FMALFEGKEVAKAASAEDLAALPIDERLQRRIVDGVREGLEDDLAEAMQARSPLEIIN
                     EVLLAGMKTVGDLFGAGEMQLPFVLQSAEVMKAAVAVLEPHMEKADQGGKGRVVLATV
                     KGDVHDIGKNLVDIILTNNGYTVHNIGIKQPIATIIEKAEEVGADAIGMSGLLVKSTI
                     VMRDNLEELNARGLESYPVLLGGAALNRTYVEGDLRQLYKGRVFYCRDAFAGLRVMDQ
                     LRSEREQGIEAEKEPVRAPRVAPARPRGPAPAPPGTDELARSEVATDVPVPTAPFLGE
                     ARVVKGIPVRDLVPYLNESALFRGQWQLRPVGGKEGWRHTLETEARPRLRALLDEAIA
                     TQILRPAVVYGYFPCNGDGNDVVVWDGDGRERVRFTFPRQRRDRRLCIADFFRPIASG
                     EPDVIGMHVVTMGLAASEATAKLFADNRYREYLELHGVTVEMAEALAEYWHQRVRQEL
                     GIAGDDAADMEDLFDQGYRGSRYSFGYPACPDLEEQTKLFELLQPGRIGIELSEEFQL
                     HPEQSTSAIIVHHPEAKYFNAR"
BASE COUNT          661 a         1978 c         1952 g          775 t
ORIGIN      
        1 gccgtcgagg gcgcaggcgc cgtggtcggc ggccagagcg cccagagcct cgacctggcc
       61 agggcctcgg cccgcgaccg caacgtcgtc atccccctgg tcctgctggt cgtgctcgtc
      121 atccttggcg tgctgctgcg ggcggtggtg gcgccgctgg tcctgatcgc caccgtgatc
      181 gtctcgttcg cggccgccct gggggcgagc gcgttcgcct tcgaccgcct cctcgggttc
      241 gggggcgccg acccgtcgct cgccctgctg gcgttcatct tcctggtcgc gctcgggatc
      301 gactacaaca tcttcctgat gagccgggtc cgggaggagt ccgagcgcct cggcacccgc
      361 gagggcaccc tcaggggcct ggccgtcacc ggcggggtca tcacctcggc cgggatcgtg
      421 ctcgcggcca ccttctcggt cctggccgtg ctgccgttcg tgcccctgat cgagatcggc
      481 atcgtggtcg ccttcggcgt cctgctcgac accctggtcg tccgctcgat cctcgtgccc
      541 gccctgaccc tcgacatcgg cccccgcatc tggtggccga gcaggctggc ggccgcgcgt
      601 cagccctcgg ccgacgaggt cctggacgac tcctcgatcc gggcccggag ccgctccagc
      661 tccgactgaa gccgctccag ctcgtcgtcc aggtagtcgc gggtcggcat ctcgccaagg
      721 gccaggcgga tcgcggccag ctcccgggcc aggtactcgg tgtcggcctg ggtgcggccg
      781 ctggtccggc ggtcacgctc ggtctcctgg cggtcccgct ccgcctggcg gttctgggcg
      841 agcaggatca gcggggccgc gtacgacgcc tgcagcgaca gggccagggt gagcaggatg
      901 aacgggtacg ggtcgaaggc ggcctcgcgg ttgcggaaca gcacgttcca ggcgacccag
      961 agcgacacca ccgccgtctg gaacaccagg tagcggccgg tgccgaggaa ccgggcgatg
     1021 gcctcggaga agcgcccgaa cgcctccggg tcgtagtggg gctggagctg gagccgggcc
     1081 ggctggcgcg gcgcccccag cgaccaggcg cgccggtccc ggcccggccg gaccctcacc
     1141 ggaccgctcc cgcgccccgc cgccaggcct cgggcagcag gtggtcgagc acgtcgtcga
     1201 tcgacaccgc ccccagcagg cggccctggg ggtcgcagac cggggcggcc agcaggtcgt
     1261 aggtggccag gtactcggcc acccggcggg tcggctcctc cggggcgatg cagtccaggt
     1321 cggtgtcgac gcaggacccc agctcctcgc tgggcggctc ccgcagcagc cgctgcaggt
     1381 aggcgacgcc caggtagcgg ccggtcgggg tctgggtcgg gggccggacc acgaacacct
     1441 gggcggccag ggcgggcggc aggtcggggt cgcgcacccg ggccagggcc tcggccacgg
     1501 tgtcggcggc ccgcatgatc accggctcgg tggtcatcag ccccccggcc gtctcctcgt
     1561 cgtagaccag cagccgccgg accggctcgg cctcgtcggg caccatcatg gccagcagcc
     1621 gggcccggtc ggcctcggac agctcgccca gcaggtcggc ggcgtcgtcg gggtccatgg
     1681 cctcgagcac gtcggccgcc cgctcgtcgc ccagcccggc cagcaggacc gcctggagct
     1741 cctcgggcag ctcctccatg gcctcggcca gccggtcgtc gtccagcccg gccaccacct
     1801 cctgccggga atggacgggc agggcctgga gggcggcggc caggtcggcc gggcgcaggt
     1861 tggccagggc ggccacccgc cgctcggccg tctcggccgg ctccagcccg gccacctgct
     1921 cccagggcag ccgcaggtgc ttggcccgcc gccacggccg cagccccccg cccggctcga
     1981 gcacgtccag cgcggtcacc acccactcct cccgcaccct gccgatggcc acgtcgttca
     2041 gccgcagctg ccggcccgtc tcccggtgcc gcacccgggc gtccagcagc tccccgacga
     2101 ccagggtctc cccctcccgc tgctcgaacg gccgcaggtt gacgctcccg gtggagaggg
     2161 tcgccccgct ctcgtccagc tcccggaccc gccccacccc gaggaagatg ggccgccgct
     2221 gcaccatcac cgtgaacccg agcaccggcg gcggcgcccc cgactcccgc ctggcgatga
     2281 ccacgtcggc gatccgcccg atggtctccc cggtgggcgc cagcaccggc aaccgcgcca
     2341 accgcgagac gaacagctgc tcgctccctg cggccacggc tcacctcgcg ttgaagtact
     2401 tggcctctgg gtggtggacg atgatggccg aggtcgactg ctcgggatgg agctggaact
     2461 cctcggagag ctcgatgccg atgcgcccgg gctggagcag ctcgaacagc ttggtctgct
     2521 cctcgaggtc ggggcaggcc gggtagccga aggagtagcg ggagccgcgg tagccctggt
     2581 cgaacaggtc ctccatgtcg gcggcgtcgt cgccggcgat gcccagctcc tggcggaccc
     2641 gctggtgcca gtactcggcc agggcctcgg ccatctcgac cgtgacgccg tgcagctcca
     2701 ggtactcgcg gtagcggttg tcggcgaaca gcttggccgt cgcctccgag gcggccaggc
     2761 ccatggtgac cacgtgcatg ccgatcacgt cgggctcgcc cgaggcgatc ggccggaaga
     2821 agtcggcgat gcacagccgc cggtcgcgcc gctggcgggg gaaggtgaag cggacccgct
     2881 cgcggccgtc gccgtcccag acgaccacgt cgttgccgtc gccgttgcag gggaagtagc
     2941 cgtagacgac ggccgggcgc aggatctggg tggcgatggc ctcgtccagc agggcccgca
     3001 ggcggggccg ggcctccgtc tccagggtgt gacgccagcc ctccttgccg ccgaccgggc
     3061 gcagctgcca ctggccccgg aacagggccg actcgttcag gtacggcacc aggtcgcgga
     3121 cggggatgcc cttgaccacc cgggcctcgc ccaggaaggg cgccgtgggc acgggcacgt
     3181 cggtggccac ctcggagcgg gccagctcgt cggtgccggg cggcgccggg gcggggccgc
     3241 gcggcctggc cggcgcgacc cggggcgccc gcaccggctc cttctcggcc tcgatgccct
     3301 gctcgcgctc ggagcggagc tggtccatga cccgcagccc ggcgaaggcg tcgcggcagt
     3361 agaacacccg gcccttgtag agctggcgca ggtccccctc gacgtaggtg cggttcaggg
     3421 ccgccccgcc cagcagcacc ggataggact ccaggccgcg ggcgttcagc tcctccaggt
     3481 tgtcgcgcat cacgatggtc gacttgacca gcagcccgga catgccgatg gcgtcggccc
     3541 ccacctcctc ggccttctcg atgatggtcg cgatcggctg cttgatgccg atgttgtgga
     3601 ccgtgtagcc gttgttggtg aggatgatgt cgaccaggtt cttgccgatg tcgtggacgt
     3661 cgcccttgac ggtggccagc accacccggc ccttgccgcc ctggtcggcc ttttccatgt
     3721 gcggctccag cacggccacg gccgccttca tgacctcggc cgactggagc acgaacggca
     3781 gctgcatctc gcccgcgccg aacaggtccc cgaccgtctt catcccggcc agcaggacct
     3841 cgttgatgat ctccaggggg ctgcgggcct gcatggcctc ggccaggtcg tcctccaggc
     3901 cctcgcggac cccgtcgacg atgcggcgct ggaggcgctc gtcgatcggc agggcggcca
     3961 ggtcctcggc gctggccgcc ttggccacct ccttgccctc gaacagggcc atgaacttct
     4021 gcagcgggtc gtagccctcg cggcgccggt cgtagatcag gtcgagggcg acctggcgct
     4081 gctcgtcgtc gatgcggtgc atgggcagga tgcgggccgg ggcgacgatc gccgagtcca
     4141 gcccggcctc gcgggcctcg tgcaggaaca ccgagttgag cacctggcgg gccgccgggg
     4201 ccaggccgaa ggagacgttg gagacgccca ggatggtccg gaccccgggc agctcggcct
     4261 tgatccgccg gatcgcctcc agggtggcca cggcgtcgcc gcgcaggtcc tcctggcccg
     4321 acccgagcgg gaaggtcagg gcgtcgaaca ccaggtcctc ggcggccagg ccgtgcttgt
     4381 cgacggccag gtcgtggatc cggtgggcga tgcgcagctt ccagtcgacg tcgcgggcct
     4441 ggccctcctc gtcgatcagc agggcgacca gggcggcccc gtactgcctg gccatcgggc
     4501 agacggcgtc catgcgggcc tcgccgtcct ccaggttgat ggagttgatg atggcccggc
     4561 cgccgaggcg cttgagcccg gcctcgagca cgtcggcctg ggtcgagtcg agcatgatgg
     4621 ggacggtcga ctgctgggcc aggcgggcgg tcaggacgtc catgtcgggc acgccgtcgc
     4681 ggcccacgta gtcgacgcag acgtccagga cgtgggcgcc ctcgcggatc tgctccttgg
     4741 cgatgccgac catgccgtcc aggtcgccct cgagcatcag gtcgcggaac ttgcgcgacc
     4801 cgttggcgtt gcagcgctcc cccaccgtca gataggtggt gtcctggtcg agcgggaccg
     4861 actggtagag gctggacagg ctgggctcgt gcagcggctc gcgggggacg ggggcgcgcc
     4921 cgcccagggc ctccaccacc tggcggatgt gctcgggggt ggtgccgcag cagccgccga
     4981 cgatgccgag cccgaactcg gccgcgaagc gggtgtgggc ctcggccagc tcctccgggg
     5041 tcagcgggta ccagggcttg ccgtcgcgca gctcgggcag gccggcgttg ggcaggcacg
     5101 acagcggcgt gcgggcgtgc tgggccaggt agcgcaggtg ctcgcccatc tcggccgggc
     5161 cggtggcgca gttcatcccg atcacgtcga ccccgagcgg ctcgatcgcg gtcagggcgg
     5221 cggcgatgtc cgacccgagc agcatctggc cggtcgtctc caccgtcacc tgggtgatca
     5281 gcgggacctt cttgttggct cgcctcatgc cggcgaacgc ccccgccacc gccaccttga
     5341 cctggaggag gtcctggcag gtttcg
//
LOCUS       DASSWR010000203         4126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_91211,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000203 DASSWR010000000
VERSION     DASSWR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91211"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1527)
                     /locus_tag="VFO47_03365"
     CDS             complement(223..1527)
                     /locus_tag="VFO47_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787767.1"
                     /translation="MFRPRTRVVLAWALWLVTFGCCAAGLAVTLAIYRPLTLGVLVEG
                     ALYAFFFVLGFATVGLVLTLRRPQNPIGWLYGAAGLAWAYTVPLEPWVDQLIREHQAL
                     PFAAKVVVATGSLGWAPPIALGVTLPALLLPNGHLRSRRWRLVVVASVTGAVLATVAG
                     TLAPGPLEERGIDNPFGLAGPAGSVAATLTLVGVLLHWWSLPPAAVCVVLRFRSSHGV
                     ERQQMRWVAAGAAAAVAGLVVSVPSGLGVAPDWISHVVYPALLCPPVAIGVAVLRYRL
                     YDLDRIVSRTLAYGLLTVLLGLGYAAVVLGLGRLLPKSSSLVVAGATLGRGGRVRAPA
                     PPRPGPGRPALQPAPLRRRRHRRGVRGPPPRPGRPGRAARRAAGRGRPDHAADPGLAL
                     APPRPLGLGRKGSGRRGHRLCSGRRRPAPDLLPGAHATGPRR"
     gene            1682..2167
                     /locus_tag="VFO47_03370"
     CDS             1682..2167
                     /locus_tag="VFO47_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HET8787768.1"
                     /translation="MTTTITDADLAELARPFPTDFVERKDGSDYVAHHVVNQRLLSIV
                     GPFDFELVQVIRGDVAAVAPDPSARSRRARAGTPALQNVVVGGIWRLTCSIDGRQVRV
                     EEVGDVGDVHNWPHDGARLKDAASDALKRCAMRLGLGLHLWAQEHYFLDQQLRAQHRT
                     E"
     gene            complement(2171..>4126)
                     /locus_tag="VFO47_03375"
     CDS             complement(2171..>4126)
                     /locus_tag="VFO47_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8787769.1"
                     /translation="PELPEGGGWLLAELPGASAAEAEAAARRLVAVAGRDALATRVLT
                     DPAETAAIWRIREDGAGFGGRSPAGAPAWAGWEDAAVPPERLGAYLRDFDALLADHGL
                     GGIPYGHFGDGCLHVRIDFPFDRPGGRAVYRSFVERAAELVGGYGGSMSGEHGDGRAR
                     SELLPRMFEPAAIEAMAAVKAVFDPRDLLNPGVLVRPRPLDADLRRTMVGPVTEGLGF
                     RYPSDGGDLTSAVHRCTGIGRCVADNTGAGGVMCPSWLATRDEKDTTRGRSRVLQEAV
                     NGTLIGGLPAPEVHEALDLCLACKGCASDCPAEVDMATYKAEVLHQTYRGRRRPAAHY
                     SLGWLPRWAALAARAPRLANRLLATPFLAAAGRRLAGVDPRRPLPAFAPQTLRAWWRN
                     RPPAGSAPATGPRVLLWPDTFTNHFTPAAGQAAVRVLEAAGYRVDLPDEPLCCALTWV
                     STGQLDTARRTLRHTVDRLAALLEGDPGGPAGAGGGGGGAVLVGLEPSCLAVLRHDAL
                     ALLGADDPAAAVVARAAATLAEVLAGTPGWAPPDLGGVVAVAQPHCHQHAVLGWEADR
                     ALLARAGTRVTAVGGCCGLAGNFGAERGHYEVSVAVAETALLPAVRAAPPDAVLLADG
                     FSCRTQLDHLAGRRALHLAELLDPGGR"
BASE COUNT          550 a         1552 c         1605 g          419 t
ORIGIN      
        1 ggcggggtcg cgggatccgc cgcctcggac ttggccagga gctggcggcg ggcggtggtg
       61 tagctctcgg acgtcttgtc catgcgggcg cggacgcggc ccttgaacga cttctgcttg
      121 gtcatcaggt gctcctccgc acgcgggtgg cgcgggaccc acgctcccgc cgcccccgcg
      181 acgagggcac tcgaccagcg tcccgaggac tcgaggtccc ctttacctcc gcgggccggt
      241 ggcgtgagca ccggggagga ggtcgggcgc gggacggcgc cggcccgagc atagccgatg
      301 gccccggcga ccggaaccct tccgccccag ccctaggggg cggggcggag ccagagcgag
      361 gcccgggtcg gctgcatggt ctggtcgacc acggccagca gctcgccgtg cagcgcgtcc
      421 aggtcgacct ggtcgcggag gcgggccgcg aacgcctcga cggtgccggc ggcgtcgtag
      481 cggcgccggt tgaagcgccg gtcgaccagg tcctggacgc ggcggcgcag gggcgcgaac
      541 gcggccacca cggccaaggg tggccccggc cacgaccagg ctcgagctct tgggcaggag
      601 ccggccgagc cccagcacca ccgcggcgta gccgaggccg agcagcaccg tcagcagccc
      661 gtaggccagg gtccggctga cgatccggtc caggtcgtac agccggtagc gcagcaccgc
      721 caccccgatc gccaccggcg ggcacagcag cgccgggtag acgacgtgcg agatccagtc
      781 gggcgcgacc ccgagcccgc tcggcacgga cacgaccagc ccggccaccg cggccgccgc
      841 cccggcggcg acccagcgca tctgctggcg ttcgaccccg tgggaggagc ggaaccggag
      901 caccacgcag accgccgccg gcggcaggct ccaccagtgg agcaggacgc cgacgagcgt
      961 gagcgtcgcg gcgacggagc cggccggacc ggccagcccg aacgggttgt cgatcccccg
     1021 ctcctccagc ggccccgggg cgagggtgcc ggccaccgtg gccaggacgg ccccggtgac
     1081 gctggcgacc acgaccagcc gccagcggcg cgaccggagg tggccgttgg gcagcagcag
     1141 ggccgggagg gtcaccccga gggcgatcgg cggcgcccag ccgaggctcc cggtcgccac
     1201 cacgaccttg gcggcgaacg gaagggcctg gtgctcgcgg atgagctggt cgacccaggg
     1261 ctccagcggg acggtgtagg cccaggcgag gccggccgcc ccgtacagcc agccgatcgg
     1321 gttctgcggc cgccgcaggg tcagcaccag cccgacggtg gcgaacccga gcacgaagaa
     1381 gaacgcgtag agggcgcctt cgaccagcac gcccagggtg agcggccggt agatggccag
     1441 ggtgacggcc agcccggcgg cgcagcagcc gaaggtgacc agccacagcg cccaggcgag
     1501 caccaccctg gttcggggcc ggaacatcac cttcctcctc gcgcgtccag gagtcccggc
     1561 gcagtctcca ggaggtgcgg tacgggagcg ttacgggctc tcccgaggca ccccggaatt
     1621 gtcacaggta ccggttacgc tgggacaacg caacgaaccc cttccccgag gactgaggcc
     1681 tgtgaccacc accatcaccg acgccgacct ggccgagctg gcccgcccct tccccaccga
     1741 tttcgtcgag cgcaaggacg gcagcgacta cgtcgcccac cacgtcgtca accagcgcct
     1801 gctcagcatc gtcggcccct tcgacttcga gctcgtccag gtcatccgcg gcgacgtcgc
     1861 cgccgtggcc cccgacccga gcgcccggag ccgccgggcc cgggccggca cgccggcgct
     1921 ccagaacgtc gtcgtcggcg gcatctggcg gctcacctgc tccatcgacg gccgccaggt
     1981 ccgggtcgag gaggtcggcg acgtcggcga cgtccacaac tggccccacg acggcgcccg
     2041 cctcaaggac gccgcctccg acgccctcaa gcgctgcgcc atgcgcctcg gcctcggtct
     2101 ccacctctgg gctcaggagc actacttcct cgaccagcag ctccgcgccc agcaccgcac
     2161 cgagtagcgg tcaccgcccg cctgggtcga gcagctcggc caggtggagg gcgcggcggc
     2221 cggccaggtg gtcgagctgg gtgcggcagg agaagccgtc ggcgaggagg accgcgtccg
     2281 ggggggcggc gcggacggcc gggaggaggg cggtctcggc gaccgcgacc gagacctcgt
     2341 agtggccgcg ctcggcgccg aagttgccgg ccaggccgca gcagccgccg accgccgtga
     2401 cccgcgtccc ggcccgggcc aggagggcgc ggtcggcctc ccagccgagc acggcgtgct
     2461 ggtggcagtg gggctgggcc accgccacga ccccacccag gtcgggcggc gcccaaccgg
     2521 gcgtgcccgc cagcacctcg gccagcgtcg ccgccgcccg ggcgaccacg gcggcggccg
     2581 ggtcgtccgc cccgagcagg gcgagcgcgt cgtgccgcag caccgccagg cacgacggtt
     2641 ccaacccgac caggaccgcc ccaccgcctc ccccgcccgc cccggccggc ccaccgggat
     2701 ccccttcgag cagggccgcg agccggtcca ccgtgtggcg gagggtgcgc ctggcggtgt
     2761 cgagctggcc ggtggagacc caggtgaggg cgcagcagag gggctcgtcg gggaggtcga
     2821 cccggtagcc ggcggcctcc aggacccgga cggcggcctg gccggccgcg ggcgtgaagt
     2881 ggttggtgaa ggtgtcgggc cacagcagca cccggggacc tgtggccggg gccgagccgg
     2941 ccggcgggcg gttgcgccac caggcgcgga gggtctgggg ggcgaacgcc gggagggggc
     3001 ggcgggggtc gaccccggcg aggcggcggc ccgcggcggc caggaacggg gtggcgagga
     3061 ggcggttggc caggcggggg gcccgggcgg cgagggccgc ccagcggggc agccagccga
     3121 ggctgtagtg ggcggccggg cggcggcggc cccgataggt ctggtggagg acctcggcct
     3181 tgtaggtggc catgtcgacc tcggccgggc agtcggaggc gcagcccttg caggccaggc
     3241 agaggtcgag ggcctcgtgg acctcgggtg cgggcaggcc gccgatcagg gtgccgttga
     3301 ccgcctcctg gaggacgcgg ctgcgtcccc gggtggtgtc cttctcgtcc cgggtggcca
     3361 gccaggacgg gcacatcacc ccgccggcgc cggtgttgtc ggccacacag cgcccgatcc
     3421 cggtgcagcg gtggacggcg ctggtcaggt cgccgccgtc gctggggtag cggaacccga
     3481 gcccttcggt gaccgggccg accatggtcc ggcgcaggtc ggcgtccagc ggccgcgggc
     3541 gcaccagcac ccccgggttg agcaggtcgc gggggtcgaa gacggccttg acggcggcca
     3601 tggcctcgat cgcggccggc tcgaacatcc gcgggagcag ctcggaccgg gcccgcccgt
     3661 cgccgtgctc ccccgacatc gacccgccgt acccgccgac cagctcggcc gcccgctcga
     3721 cgaacgaccg gtagaccgcc cgcccgccgg gccggtcgaa cgggaagtcg atgcgcacgt
     3781 gcaggcagcc gtcgccgaag tggccgtagg ggatgccgcc gaggccgtgg tcggccagga
     3841 gggcgtcgaa gtcgcgcagg taggcgccga gccgctcggg cgggacggcc gcgtcctccc
     3901 agccggccca ggccggcgcc ccggccgggc tgcgcccgcc gaacccggcc ccgtcctcgc
     3961 ggatgcgcca gatggcggcc gtctcggccg ggtcggtcag gacccgggtg gcgagcgcgt
     4021 cccggccggc cacggcgacc agccgccgcg cggccgcctc ggcctcggcc gccgaggcgc
     4081 cgggcagctc ggccaggagc cagccgccgc cctcgggcag ctccgg
//
LOCUS       DASSWR010000204         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_601620,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000204 DASSWR010000000
VERSION     DASSWR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601620"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..967
                     /locus_tag="VFO47_03380"
     CDS             236..967
                     /locus_tag="VFO47_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HET8787770.1"
                     /translation="MLRGIGMFLGTPGIRLLGIVPVLLAALLVLSLLGLLVVYLDELA
                     DTLTPFADRWDESSRTLVRVGAGLALLLGSTAVLVVSFTVIAQIIGQPFYERISDRIE
                     HRLGAPPAGAGTPWWQTFPRASLESALLLAMTLACTAPLFVLGLFPVLGQTVVPLLQA
                     LVAGFFLAVELLAIPLERRGLHLAGRLRFVWRHRAQTLGFGITAFLLFLVPLMNVLAM
                     PGAVVGATLLVRRLSGDQDGRPGPP"
     gene            complement(930..1157)
                     /locus_tag="VFO47_03385"
     CDS             complement(930..1157)
                     /locus_tag="VFO47_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787771.1"
                     /translation="MEVQIPGMEGIETGTEVKVRKPGQGERGVWRVTGEVANSGPDDP
                     AYDLTNLVSGRRRIFRASRLTVVRAARPGRR"
     gene            complement(1239..1952)
                     /locus_tag="VFO47_03390"
     CDS             complement(1239..1952)
                     /locus_tag="VFO47_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HET8787772.1"
                     /translation="MRSELFGRNLEASTPGEVFALQNSRMLRVALDGEVMARQGSMIA
                     YQGQVDFEYQGAGGIGRMLKKAVTGEGVPLMRCRGKGDVFFANYAAEVHLLRLEGDAL
                     SVNGHSILAFEPTLTWDIRRIEGASMFAGGLFNTILQGTGWAAITAHGTPVVLQTDAP
                     TYVDAQAAIAWSANLQTSIHRTMKAGALIGRGSGEAVQLAFAGSGFVIVQASEGEGVA
                     PHNHGGGGGGGGGGLLDFG"
     gene            2080..>3002
                     /locus_tag="VFO47_03395"
     CDS             2080..>3002
                     /locus_tag="VFO47_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET8787773.1"
                     /translation="MTADPGPEPTFDLDLYLSLPRVAGLALSPDGRRLVTSVATPGPD
                     SKRFASALWELDPAGQAAPRRLTRSAPGESGAAFLPDGSLLFTSTRDDPEAAGDAKPD
                     GEVARLWLLPAGGGEARPVAATPAGIDLHRVARDAGTVVYRSGVHPGADDLEADERRD
                     KARGDAGVSALLFETYPIRYWDHYLGPRAPRLFAADPPPAGDGRMGPGRPLTPAPGRA
                     LDEQDVAVTPDGATVLCGWATGEVTNPRSLLVAIDVSSGERRVLLDDPESSVGRIACS
                     PDGRLAVCERIRHGDPGHPPDLGLWLVDLDSG"
BASE COUNT          333 a         1157 c         1100 g          412 t
ORIGIN      
        1 atgtccgccc gacgaccccg tccgacccgg agcgctcgag gccccggcgc cgatgagcaa
       61 cccaccggac ccgccgcccg gccgccctcc ggggccggag cggcccggcg cggtgcggcc
      121 cgccgggtgg accacgccgg cggggtgggc gggcgcaccc ccgcccaccc cggcgccagg
      181 gccggcaccg gggccgggac ggggggtcgg ggggccgctg gccggagggg cggacgtgct
      241 ccgggggatc gggatgttcc tgggaacccc cgggatccgg ctgctgggga tcgtgccggt
      301 gctgctggcg gcgctgctgg tgctgtccct gctcggcctg ctggtcgtct acctcgacga
      361 gctggccgac accctgacgc cgttcgccga ccggtgggac gagagcagcc ggaccctggt
      421 ccgggtcggg gccgggctgg ccctgctgct ggggtcgacg gccgtgctgg tggtgtcgtt
      481 cacggtcatc gcccagatca tcggccagcc cttctacgag cggatctcgg accgcatcga
      541 gcaccggctc ggcgccccac cggccggggc cggcaccccc tggtggcaga ccttccccag
      601 ggcctcgctg gagtcggccc tgctgctggc catgaccctg gcctgcacgg cgcccctgtt
      661 cgtgctgggg ctgttcccgg tgctcggcca gacggtcgtg cccctcctcc aggccctggt
      721 ggccgggttc ttcctggccg tggagctgct ggccatcccg ctggagcggc gcggcctgca
      781 cctggccggg cggctgcggt tcgtgtggcg gcaccgcgcc cagaccctcg ggttcggcat
      841 caccgccttc ctgctgttcc tggtcccgct gatgaacgtg ctggccatgc ccggggcggt
      901 cgtcggggcg accctgctgg tgcggcggct cagcggcgac caggacgggc ggcccggacc
      961 accgtgagcc gggaggcccg gaagatgcgg cggcggccgc tgaccaggtt ggtcaggtcg
     1021 taggcggggt cgtccgggcc cgagttggcc acctcaccgg tgacccgcca cacaccccgc
     1081 tccccctgcc ccggcttccg gaccttgacc tcggtacccg tctcgatgcc ctccatccca
     1141 gggatctgga cctccacgcc agcctccttc acggcggttc gaactcatgt tcgattctac
     1201 ccttccggtg tgtcaggagc gacccgatcc accgcgggtc atccgaagtc gagcaggccg
     1261 ccaccgccgc caccgccgcc gccgccgtgg ttgtgggggg cgacgccctc gccctcgctg
     1321 gcctggacga tcacgaaccc ggagccggcg aaggccagct ggaccgcctc gccgctgccc
     1381 cggccgatca gggcgccggc cttcatggtc cggtggatgc tcgtctggag gttggcgctc
     1441 caggcgatgg cggcctgggc gtcgacgtag gtcggggcgt cggtctgcag caccaccggg
     1501 gtgccgtggg cggtgatggc cgcccagccg gtgccctgga ggatcgtgtt gaacagcccg
     1561 ccggcgaaca tcgaggcccc ctcgatccgg cggatgtccc aggtgagggt gggctcgaag
     1621 gcgaggatgc tgtggccgtt gacgctgagg gcgtcgccct ccagccgcag gaggtggacc
     1681 tcggcggcgt agttggcgaa gaacacgtcg cccttgccac ggcagcgcat cagcggcacg
     1741 ccctcgccgg tgacggcctt cttgagcatg cggccgatgc cgcccgcccc ctggtactcg
     1801 aagtcgacct ggccctggta ggcgatcatc gacccctggc gggccatcac ctcgccgtcc
     1861 agggcgaccc gcaacatgcg cgagttctgc agggcgaaga cctcgccggg ggttgacgcc
     1921 tcgaggttcc ggccgaacag ctcgctgcgc acctgaccat ctccttgctc gcacggtcga
     1981 cgcgggcact cgtacccgaa ccggacggcg atgtccagca ccctcggcga ccggccggtt
     2041 cccgttgcgc ggccgccgga gggcttctag attcgggcca tgaccgccga cccgggtccc
     2101 gagccgacgt tcgacctcga tctctacctg agccttccgc gggtggccgg gctggccctc
     2161 tcccccgacg gccgccggct ggtcacctcg gtggccacgc cgggaccgga cagcaagagg
     2221 ttcgccagcg ccctgtggga gctcgacccg gccggccagg cggccccgcg gcggctcacc
     2281 cgctcggccc ccggcgagtc cggcgccgcc ttcctgcccg acgggtcgct gctgttcacc
     2341 tccacccgcg acgaccccga ggccgccggc gacgccaagc cggacggcga ggtggcccgc
     2401 ctgtggctgc tgccggccgg cggcggggag gcccgcccgg tggccgccac cccggccggg
     2461 atcgacctgc accgggtggc ccgcgacgcc ggcacggtcg tgtaccgcag cggcgtccac
     2521 cccggggccg acgacctgga ggccgacgag cgccgcgaca aggcccgcgg cgacgccggg
     2581 gtcagcgccc tgctgttcga gacctacccg atccgctact gggaccacta cctggggccg
     2641 agggcgccgc ggctgttcgc ggccgacccg cccccggccg gcgacggccg catgggcccg
     2701 ggccggcccc tgaccccggc cccgggccgg gccctggacg agcaggacgt ggccgtcacc
     2761 cccgacggcg ccaccgtgct ctgcggctgg gccaccggcg aggtcaccaa cccgcgctcg
     2821 ctgctggtgg cgatcgacgt ctccagcggc gagcggcggg tcctgctgga cgacccggag
     2881 tcgtcggtgg gccggatcgc ctgctccccc gacgggcggc tggcggtgtg cgagcggatc
     2941 cgccacggcg accccggcca ccccccggac ctcggcctgt ggctggtcga cctggacagc
     3001 gg
//
LOCUS       DASSWR010000205         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_262582,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000205 DASSWR010000000
VERSION     DASSWR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262582"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFO47_03400"
     CDS             complement(<1..395)
                     /locus_tag="VFO47_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8787774.1"
                     /translation="MNQWALPTVAVALLAYGAVSGRLRSTPISQAMVFVALGLLAGNR
                     VLHLVEADTANQYVRHLAEATLALVLFIDAVRVNRGRLRHESALPTRLLGVGLPLTIV
                     AGTLAALVLFPQLDLWTAAALATMVAPTDA"
     gene            complement(447..917)
                     /locus_tag="VFO47_03405"
     CDS             complement(447..917)
                     /locus_tag="VFO47_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787775.1"
                     /translation="MTSRQDFTGEEWVRIRRAPLVAGVAISLADPGGPIEVAKETMAT
                     LRSATLPPSQEELLASVALDVQALAQQKQNPLGDFKPRGGQQVLEELRAVNEIVTAKA
                     TPEEVEAFRRWLVAAAQAAADAGKEGGFMGFGAERVSAGERQMLEQVRAAVGIT"
     gene            1051..1500
                     /locus_tag="VFO47_03410"
     CDS             1051..1500
                     /locus_tag="VFO47_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HET8787776.1"
                     /translation="MSDEQLEELGPIDYVVLEWPGAYPDTSEVQPLLLDLVSRGIIRI
                     LDIAFVTKDEDGSVSGLELGDLKQVTAAFAEFEGAASGLLGFEDLQEAASILEPGTSA
                     AVLVWENRWAAPVAAALRRSGGQLAASGRLPVQAILAALDAVEATER"
     gene            1510..1794
                     /locus_tag="VFO47_03415"
     CDS             1510..1794
                     /locus_tag="VFO47_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010842346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET8787777.1"
                     /translation="MPGLLRGVARTAAIAGTATAVSNRVSRRQANRWSRQGAYDQPAY
                     EEPPPQYAAAPEPAPQQDMVQQLKDLAELKDQGILTEEEFAAQKARILGS"
BASE COUNT          291 a          720 c          731 g          274 t
ORIGIN      
        1 gcgtcggtgg gggccaccat ggtcgccagc gcggccgcgg tccacaggtc cagctggggg
       61 aacaggacca gggcggccag ggtgccggcc acgatggtga ggggcaggcc gaccccgagc
      121 agtcgtgtcg ggagggcgga ctcgtgccgt agccggccgc ggttgacccg gaccgcgtcg
      181 atgaacagca ccagggccag ggtggcctcg gccaggtggc ggacgtactg gttggccgtg
      241 tccgcctcga cgaggtgcag gacccggttg ccggccagca ggccgagggc gacgaagacc
      301 atggcctggc tgatcggtgt ggagcgcagc cgtcccgaca cggcgccata ggcgagcaac
      361 gccacggcga cggtgggcag ggcccactgg ttcatggtct ctcctcagga cagcgcgcga
      421 cccaccccag gcgccgcctc gaccggtcag gtgatcccga ctgcggcgcg cacctgctcc
      481 agcatctgcc gctctccggc gctcacccgc tcggcgccga agcccatgaa gccgccctcc
      541 ttcccggcgt cggcggccgc ctgggcggcg gccaccagcc atctgcggaa cgcctcgacc
      601 tcctcgggcg tggccttggc cgtcacgatc tcgttgacgg cgcgcagctc ctccagcacc
      661 tgctggccgc cgcgcggctt gaagtccccc agcggattct gcttctgctg ggccagcgcc
      721 tggacgtcga gggccaccga cgccagcagc tcctcctggc tggggggaag ggtggccgac
      781 cgcagcgtcg ccatcgtctc cttggccacc tcgatcggcc caccggggtc cgcgagcgag
      841 atcgccacgc cggccacaag gggggcgcga cggatccgta cccattcctc gccggtgaag
      901 tcctgcctgg acgtcatagc cacctcctgg acgggggcat cagcctagac gagactgctc
      961 ggcaccggat cacccggttc ggatgatgcg cgccccggcc gctgcagcga ccatggcgtg
     1021 gccgatggtg aaggaggagg aggagacgcc gtgagcgacg agcagctgga ggagctaggc
     1081 ccgatcgact acgtggtcct ggagtggccg ggcgcgtatc ccgacaccag cgaggtccag
     1141 ccgctgctgt tggacctggt gagccggggc atcatccgca tcctcgacat cgcgttcgtg
     1201 accaaggacg aggacgggtc ggtgtcaggg ctcgagctgg gcgatctcaa gcaggtgacc
     1261 gcggccttcg ccgagttcga gggtgccgcc tcgggcctgt tgggcttcga ggacctgcag
     1321 gaggccgcca gcatcctgga gccaggcacc tcggccgcgg tcctggtctg ggagaaccgc
     1381 tgggccgcgc ccgtggccgc ggcgctgcgc cgatccggcg ggcagctggc ggccagcggc
     1441 cgcctgcccg tccaggcgat cctggccgcg ctggatgcgg tcgaggccac cgagcgataa
     1501 ggagcaccca tgccaggact actccgcggg gtcgcccgca ccgccgccat cgctggcacc
     1561 gccaccgcgg tcagcaaccg tgtctcgcgc cggcaggcca accggtggtc gcgccaggga
     1621 gcctacgacc agcccgccta cgaggagccg ccgccccagt atgcggcggc gcccgagccg
     1681 gcacctcagc aggacatggt ccagcagctc aaggatctgg ccgagctgaa ggaccagggc
     1741 atcctcaccg aggaggagtt cgcggcccag aaggcccgca tcctcgggag ctgacaagcc
     1801 gctcgtccgg gccggcggcg ggatcgtctg gcgccgtggc gagggcggcg cggtcgagat
     1861 cgtcctcatt caccggccgg gatgagcgct gccgtcaccc tgccgcctgg gattccatga
     1921 gaccgggcgc cggctttcca cctcgacccg ttgtgacgga tgctgggcct cctgcgacgg
     1981 ccgtaggccg accctcgtcc cggatggatc gctgtc
//
LOCUS       DASSWR010000206         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1109913, whole genome shotgun sequence.
ACCESSION   DASSWR010000206 DASSWR010000000
VERSION     DASSWR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109913"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFO47_03420"
     CDS             <1..1137
                     /locus_tag="VFO47_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8787778.1"
                     /translation="VEAVGRQAADLLHYSASDFYLPIYSEYAEALAATAPMTGPVRAF
                     LTNSGTEAVEGALKLVRAATGRQYVISFYGAFHGRSYGSVSLTASKAKYHAGFGPLLP
                     SVLHAPYGYCHRCPLNLTFPDCGHATVDWLEQTLFRYEVDPGEVAAVFVEPIQGEGGY
                     ITPPPGWLAKLKALCQRHGILFVADEVQSGMGRTGRMWAVEHTGVEPDVLIAAKGIAS
                     GLPLGAFMARDELWRWPKGAHGSTYGGSPVPCAAGLATLEVLRDEKLLDNATAQGELL
                     LEGLERLRTRFGWLVEDVRGVGLMLGVEFPTGELAEEVQLACFRRGLLVLEAGESAVR
                     LSPPLVVTADQCHTALRLFTEAVEEVAAGAAATATSSPERAAGP"
     gene            1134..2054
                     /locus_tag="VFO47_03425"
     CDS             1134..2054
                     /locus_tag="VFO47_03425"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET8787779.1"
                     /translation="MSQSKVASMRDAVAELVRDGDTLAIEGFTHLISFAAGHEVIRQR
                     RRDLTLCRLTPDVVYDQMIGAGVARKLVFSWMGNPGVGSLHAIRRRVEHHDPAPLELE
                     EYSHFGMVGRYAAGAANLPFWPLRSYFETDLPKVNPNIRPVTSPFGGEPVFAVPPLNP
                     DVAVVHAQRADAAGNTQIWGLIGCQKEAAFAASRVIVVVEELVDAEVVRADPNRTLIP
                     GIKVDAVVVCPRGAHPSYAQGYYDRDNRFYLEWDRISRDPAVLAAWLDEWVHGTSDHS
                     EYVEKLGAERWAELTPAPALSGSVDYGDYR"
     gene            2078..>2562
                     /locus_tag="VFO47_03430"
     CDS             2078..>2562
                     /locus_tag="VFO47_03430"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET8787780.1"
                     /translation="MLVVAGARALAGRRVCFVGIGLPNIAVALAQRTVAPDIELVYES
                     GVYGARPVRLPLSIGDPCLVTGATAAMSMVELFSYYLQGGLVDVGFLGAAQLDRFGNI
                     NTTVIGDYEQPKVRLPGSGGACEIAINAREVFVLMRQSARSFVERIDFRTSPGNLGGA
                     AL"
BASE COUNT          337 a          947 c          912 g          366 t
ORIGIN      
        1 gtcgaggcgg tcggccgcca ggccgcggac ctgctgcact actcggccag cgacttctac
       61 ctgcccatct actccgagta cgcggaggcc ctggccgcca ccgcccccat gaccgggccg
      121 gtgcgggcct ttctgaccaa ctcgggcacc gaggccgtgg agggggcgct caagctggtc
      181 cgggcggcca ccggccgcca gtacgtgatc agcttctacg gcgccttcca cggccgcagc
      241 tacggctcgg tcagcctgac cgcgtccaag gcgaagtatc acgccggatt cgggccgctg
      301 ctgccgtcgg tgctgcacgc cccctatggc tactgccacc gctgccccct caacctgacc
      361 ttccccgact gcggccacgc caccgtcgac tggctggagc agaccctgtt ccgctacgag
      421 gtcgacccgg gcgaggtcgc ggccgtgttc gtcgagccca tccagggtga gggcggctac
      481 atcaccccgc cgccgggctg gctggccaag ctcaaggcgc tgtgccagcg ccacggcatc
      541 ctgttcgtgg ccgacgaggt ccagtcgggc atgggccgga ccgggcgcat gtgggcggtc
      601 gagcacaccg gggtcgagcc ggacgtgctg atcgcggcca aggggatcgc ctccggcctg
      661 ccccttggcg cgttcatggc ccgcgacgag ctgtggcgct ggcccaaggg cgcccacggc
      721 tccacctacg gcggcagccc cgtcccctgc gccgccggcc tggccaccct ggaggtcctg
      781 cgggacgaga agctgctcga caacgccacc gcccagggcg agctgctgct ggaggggctg
      841 gagcgcctcc ggacccgctt cggctggctg gtcgaggacg tccgcggcgt cgggctcatg
      901 ctcggggtcg agttccccac cggggagctg gccgaggagg tccagctggc ctgcttccgc
      961 cgcggcctcc tcgtcctgga ggccggcgag agcgccgtcc gcctctcccc gcccctggtc
     1021 gtcaccgccg accagtgcca caccgccctg cgcctgttca ccgaggccgt cgaggaggtc
     1081 gcggccggcg cggccgcgac cgctacgtca tcgcctgaac gagcagcggg gccatgagcc
     1141 agagcaaggt cgcctccatg cgcgacgccg tcgccgagct ggtccgcgac ggcgacaccc
     1201 tcgccatcga ggggttcacg cacctgatct cgttcgcggc cgggcacgag gtgatccgcc
     1261 agcgccgccg cgacctgacc ctgtgccggc tcacccccga cgtggtctac gaccagatga
     1321 tcggggccgg ggtcgcccgc aagctggtct tctcctggat ggggaacccg ggggtggggt
     1381 cgctgcacgc catccgccgc cgggtcgagc accacgaccc ggctccgctg gagctggagg
     1441 agtacagcca cttcgggatg gtcgggcgct acgccgccgg cgcggccaac ctgccgttct
     1501 ggccgctgcg gtcctatttc gagaccgacc tgccgaaggt caaccccaac atccgcccgg
     1561 tcacctcccc gttcgggggc gagccggtgt tcgcggtgcc gcccctgaac ccggacgtgg
     1621 ccgtggtcca cgcccagcgg gccgacgccg ccggcaacac ccagatctgg gggctgatcg
     1681 gctgccagaa ggaggcggcg ttcgcggcca gccgggtgat cgtggtcgtc gaggagctgg
     1741 tcgacgccga ggtcgtccgg gccgacccca accggaccct gatccccggg atcaaggtgg
     1801 acgcggtggt ggtctgcccg cgcggcgccc atccctccta cgcccagggc tactacgacc
     1861 gcgacaaccg cttctacctg gagtgggacc ggatcagccg cgacccggcg gtgctggcgg
     1921 cgtggctgga cgagtgggta cacggcacca gcgaccacag cgagtacgtg gagaagctgg
     1981 gggccgagcg ctgggccgag ctgaccccgg ccccggcgct gtcgggttcg gtcgactacg
     2041 gggactaccg gtgacggcca cctggtccaa aagcgagatg ctggtggtgg cgggggcgcg
     2101 ggccctggcg ggcaggcggg tgtgcttcgt cggcatcggc ctgcccaaca tcgccgtcgc
     2161 cctggcccag cggacggtcg cgcccgacat cgagctggtc tacgagtcgg gggtgtacgg
     2221 ggcccggccg gtccggctgc ccctgtccat cggcgacccc tgcctggtca ccggggccac
     2281 cgccgcgatg agcatggtcg agctgttcag ctactacctc cagggcgggc tggtcgacgt
     2341 cggcttcctg ggcgccgccc agctggaccg gttcggcaac atcaacacca ccgtcatcgg
     2401 cgactacgag cagcccaagg tgcggctgcc cgggtccggg ggggcgtgcg agatcgccat
     2461 caacgcccgc gaggtgttcg tgctgatgcg ccagtcggcc cgctcgttcg tggagcggat
     2521 cgacttccgg accagcccgg gcaacctcgg cggggccgcc ct
//
LOCUS       DASSWR010000207         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_375686,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000207 DASSWR010000000
VERSION     DASSWR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375686"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFO47_03435"
     CDS             <1..751
                     /locus_tag="VFO47_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8787781.1"
                     /translation="LPGDEQAVNRVEGLFRTYASTGDQAVREQIIVAYLGLADRLADR
                     YRGSRGATPEDLRQTARVGLVAAVDRYDPDYGNPFVPYAVACVVGEIKRYLRDTSWRV
                     HVSRPVKERSLRLCKALDELPHTLGRSPTIAELASYLGISEEEALEAIEVVHTRSEVS
                     LDQQVGDEGDTSLGDRLPAAPPREELEDLLLLPGLVDSLPEPEREIVVLRFFHELDQY
                     DIAARVGCSQMHVSRLLRRALGRLRTQLLVN"
     gene            762..1217
                     /locus_tag="VFO47_03440"
     CDS             762..1217
                     /locus_tag="VFO47_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET8787782.1"
                     /translation="MAQDAVALIKADHRKVEQLFREFEKAGDRAYKTKQDLVADIIKE
                     LEVHAAIEEETYYPAVEAKARKEGKEMVAEAVEEHHVVKILLGELGSMSSEDEAFDAK
                     VTVLMENVRHHVEEEEGELLPQSEEILGKEELTRLGEEMAARKKQLGAG"
     gene            1276..1797
                     /locus_tag="VFO47_03445"
     CDS             1276..1797
                     /locus_tag="VFO47_03445"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HET8787783.1"
                     /translation="MADGESKGERLDRELMELLQELRVVIPGVQVLLAFLLTAPFQQR
                     FAQLQGSLRNAFFASIACATLATAFLIAPSAHHRLRWRAGEKERLVRIGNQMAIAGTV
                     FLAAAIVLAVYVVTNVLFTTDLALWTAAGFIVVFAGLWYVLPLAWRPSPEEKASEEGG
                     GRDQESRGASSRR"
     gene            complement(1764..2714)
                     /locus_tag="VFO47_03450"
     CDS             complement(1764..2714)
                     /locus_tag="VFO47_03450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8787784.1"
                     /translation="MAEIGYFLSSEEHGPNELVRQAQQAEEAGFRSVWISDHYHPWND
                     NQGQSPFVWSVIGGIAATTDLKVTTGVTCPTVRIHPAILAQAAATAALMLEGRFLLGV
                     GSGENLNEHVLGDRWPRVDTRLEMLEEAVEVMRKLWEGDLTSHDGRFYQVENARVYSL
                     PDTPPPVLVSALGPKAVSLAARIGDGYISTSPDSEALDSYVEQGGKGLKQGGMKVCWG
                     RDEAAARKTALELWPNTLVPGQLSQELALPSHFEQASQLVTEDKLAETIPCGPDPERH
                     LESFRQYLDAGFDEVYVSQIGDDQAGFFEFYRRELAPRLS"
     gene            complement(2735..3787)
                     /locus_tag="VFO47_03455"
     CDS             complement(2735..3787)
                     /locus_tag="VFO47_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8787785.1"
                     /translation="MRVVVTGASGNVGTSVLAALAGESAVTSVLGLARRRPEAEVTKT
                     TWATTDVVDGDLAGHFRGADVVVHLAWLIQPSHDPFAMWRTNVVGTERVLAAASQAGV
                     GAVVCASSVGAYSPGPKDRQVDEGWPTHGVATSAYSRDKAYQERLLDLFERDHPEVRV
                     VRLRPALTFKREAASEIRRFFLGPLVPGSLARAGAIPVVPRVKRLRTQAVHADDVGQA
                     YRLAVAGDARGPFNLAADPVLDAGVLAAMLDARPVPVPAAVLKAGAAATWRLHLQPVS
                     PGWLDLALQSPLLDAGRARAELGWSPRRSATDTVEELLAGFREAAGADTPPLDPDAGG
                     PLRLGEFRSGVGGADR"
     gene            3888..>5183
                     /locus_tag="VFO47_03460"
     CDS             3888..>5183
                     /locus_tag="VFO47_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET8787786.1"
                     /translation="MLRIPALGAVRRRPRHPAQNVAAAFAVGILVGTVLLLLPPARAG
                     PGGAPLHVATFTATSAVCVTGLTVVDTATYWSTLGQVIILLLVQVGGLGFMVLASLLA
                     LALSRRLGLRQRLLAQTEINVVELGETRRVLLGVAIFSVAFEVVTAAVLTGRLWLGYG
                     EPFGRAAWYGLFHSVSAFNNAGFALYGDNLVGFVDDGWVSLTVTLAVIAGGLGFPVLI
                     ELIREPGTPDRWSLHTKLTLTMTWVLLATGTLAVLAFEWSNPATFGPLDVPGKLLAGF
                     FQGANPRSAGFNTVDYAEMGETSWLVTEVLMFIGAGSAGTTGGIRVTTFAVLVLMVAA
                     EARGRSTVNAFGRRIPQGAQRQALSVTLIGLGAVVAATLAITAQSGLPLSGTLFEAFS
                     AFGTVGLTTGITPQLPVPSQNLLILLMFLGRTGPITLATA"
BASE COUNT          683 a         1903 c         1849 g          748 t
ORIGIN      
        1 cctcccgggc gacgagcagg ccgtcaaccg tgtcgagggc ctgttccgga cctacgccag
       61 caccggcgac caggccgtcc gggagcagat catcgtcgcc tacctgggcc tggccgaccg
      121 gctggccgac cgctaccggg gcagccgcgg ggccaccccc gaggacctgc gccagacggc
      181 ccgggtcggg ctggtcgcgg ccgtcgaccg ctacgacccc gactacggca acccgttcgt
      241 cccctacgcg gtggcctgcg tggtcggcga gatcaagcgc tacctgcgcg acaccagctg
      301 gcgggtccac gtctcccggc cggtcaagga gcgctccctg cgcctctgca aggccctgga
      361 cgagctgccg cacaccctgg ggcgctcgcc caccatcgcc gagctggcct cgtacctggg
      421 catctccgag gaggaggcgc tggaggcgat cgaggtcgtc cacacccgct cggaggtctc
      481 gctcgaccag caggtcggcg acgagggcga cacctccctg ggggaccggc tgccggccgc
      541 cccgccccgc gaggagcttg aggacctgct cctgctgccc gggctggtcg acagcctgcc
      601 cgagccggag cgcgagatcg tggtcctgcg cttcttccac gagctcgacc agtacgacat
      661 cgccgcccgc gtcggctgct cgcagatgca cgtatcacgc ctgctgcgcc gcgccctcgg
      721 gcggctccgc acccaactgc tggtgaactg aggaggttgc cgtggcacag gacgccgtcg
      781 cgttgatcaa ggccgaccac cgcaaggtgg agcagctgtt ccgggagttc gagaaggcgg
      841 gtgaccgcgc ctacaagacc aagcaggacc tggtcgccga catcatcaag gagctcgagg
      901 tccacgccgc catcgaggag gagacctact acccggcggt cgaggccaag gccaggaagg
      961 agggcaagga gatggtcgcc gaggccgtcg aggagcatca cgtcgtcaag atcctgctcg
     1021 gcgagctggg ctcgatgtcg tccgaggacg aggccttcga cgccaaggtc accgtcctga
     1081 tggagaacgt gcgccatcac gtcgaggagg aggaagggga gctgctccca cagtccgagg
     1141 agatcctcgg caaggaggag ctgacccgac tcggcgagga gatggcggcc cgcaagaagc
     1201 agctcggcgc cggctgatcg caggtttgcg cccccgtcta cactcaaatc cacatgaacg
     1261 ggaggtggac ggtgcatggc cgacggtgag agcaagggcg agcggctcga ccgggagctg
     1321 atggagctgc tccaggagct ccgggtcgtc atccccgggg tccaggtgct gctggccttc
     1381 ctgctcaccg cccccttcca gcagcgcttc gcccagctcc agggctcgct gcgcaacgcc
     1441 ttcttcgcct cgatcgcctg cgccaccctg gccaccgcgt tcctgatcgc cccctcggcc
     1501 caccaccggc tgcgctggcg ggccggcgag aaggagcgcc tggtgcggat cggcaaccag
     1561 atggccatcg ccggcaccgt gttcctggcc gcggccatcg tgctcgccgt ctacgtggtc
     1621 accaacgtgc tgttcaccac cgacctggcc ctctggaccg cggccggctt catcgtggtg
     1681 ttcgccgggc tctggtacgt cctgccgctg gcctggcgcc cctcccccga ggagaaggcc
     1741 tcggaggagg gaggcgggcg ggatcaggag agccgcgggg ccagctcccg gcggtagaac
     1801 tcgaagaatc ccgcctggtc gtcgccgatc tggctgacgt agacctcgtc gaagccggcg
     1861 tccaggtact gccggaagct ctccaggtgc cgctccgggt ccggcccgca ggggatcgtc
     1921 tcggccagct tgtcctcggt gaccagctgg gacgcctgct cgaagtggct gggcagggcc
     1981 agctcctggg agagctggcc gggcacgagg gtgttgggcc acagctccaa ggcggtcttg
     2041 cgggcggccg cctcgtcccg gccccagcag accttcatgc cgccctgctt gagccccttg
     2101 ccgccctgct cgacgtagct gtccagcgcc tcggagtcag gtgaggtgct gatgtagccg
     2161 tcgccgatgc gggcggccag gctgaccgcc ttgggcccga gggccgacac cagcaccggc
     2221 ggcggggtgt cgggcagcga gtacacgcgg gcgttctcga cctggtagaa gcggccgtcg
     2281 tggctggtca ggtcgccctc ccagagcttg cgcatgacct cgacggcctc ctccagcatc
     2341 tccagccggg tgtcgacccg cggccagcgg tcgccgagga cgtgctcgtt caggttctcg
     2401 ccgctgccga ccccgagcag gaagcggccc tccagcatga gggcggcggt ggccgccgcc
     2461 tgggccagga tggccgggtg gatccggacc gtcgggcagg tcaccccggt ggtgaccttg
     2521 aggtcggtgg tggcggcgat gccgccgatc accgaccaca cgaacgggct ctggccctgg
     2581 ttgtcgttcc aggggtggta gtggtcggag atccacaccg accggaagcc ggcctcctcg
     2641 gcctgctggg cctggcgcac cagctcgttc gggccgtgct cctcgctgga caggaagtag
     2701 ccgatctcgg ccatgtcgtc gctccttctc gtgatcagcg gtcggcgccg ccgacgccgc
     2761 tgcggaactc gcccaggcgc agcggcccgc cggcgtcggg gtcgagcggc ggggtgtccg
     2821 ccccggcggc ctcgcggaac ccggccagca gctcctcgac ggtgtcggtg gcgctgcgcc
     2881 ggggcgacca gcccagctcg gcccgggccc ggccggcgtc cagcaggggc gactgcaggg
     2941 ccaggtcgag ccagccgggc gacaccggct ggaggtgcag ccgccaggtc gcggccgccc
     3001 ccgccttgag caccgccgcc ggcaccggca cgggccgggc gtccagcatg gcggccagca
     3061 cgccggcgtc cagcacgggg tcggcggcca ggttgaacgg gccgcgggcg tcgccggcca
     3121 cggccagccg gtaggcctgg ccgacgtcgt cggcgtgcac cgcctgggtg cgcagccgct
     3181 tcaccctcgg caccaccggg atggcgccgg ccctggccag cgagccgggc accagggggc
     3241 cgaggaagaa gcggcggatc tccgaggcgg cctcgcgctt gaaggtcagg gcgggccgca
     3301 gccgcaccac ccgcacctcc gggtggtcgc gctcgaagag gtcgagcagc cgctcctggt
     3361 aggccttgtc gcgcgagtag gccgaggtgg cgaccccgtg ggtcggccag ccctcgtcga
     3421 cctgccggtc cttggggccg ggggagtagg ccccgaccga ggaggcgcag accacggcgc
     3481 ccaccccggc ctgggaggcg gcggccagca cccgctcggt gccgacgacg ttggtgcgcc
     3541 acatggcgaa ggggtcgtgg gagggctgga tcagccaggc caggtggacg accacgtcgg
     3601 cgccgcggaa gtggccggcc aggtcgccgt cgaccacgtc ggtggtcgcc caggtggtct
     3661 tggtcacctc ggcctcgggc cggcgcctgg ccagccccag caccgaggtg acggcggact
     3721 cgccggccag cgcggccagc acgctggtcc ccacgttccc gctggccccg gtcacgacga
     3781 cgcgcatggc tctccttctc ggccgtccct tgtggtccag ccagctaccc ctggccccgg
     3841 cccaggaaac ccacggaccg cccggcatgc cggagaatgc cggtggcatg ctccggatac
     3901 ctgcgctcgg cgccgtgcgc cgccgcccgc gacatcccgc ccagaacgtg gccgccgcct
     3961 tcgcggtcgg gatcctggtc gggaccgtcc tgctgttgct gccgccggcc cgggccgggc
     4021 ccggcggggc cccgctgcac gtggccacct tcaccgcgac ctcggccgtc tgcgtcaccg
     4081 ggctgaccgt cgtcgacacc gccacctact ggtcgaccct cggccaggtg atcatcctgc
     4141 tgctcgtcca ggtcggcggg ctcggcttca tggtgctggc gtcgctgctc gccctggccc
     4201 tgtcccgccg gctcgggctg cggcagcggc tgctcgccca gaccgagatc aacgtcgtcg
     4261 agctcggcga gacccggcgc gtgctgctgg gcgttgccat cttcagcgtg gccttcgagg
     4321 tggtgaccgc cgcggtgctc accggacggc tgtggctggg ctacggcgaa ccgttcggcc
     4381 gggcggcctg gtacggcctg ttccactcgg tgtcggcctt caacaacgcc ggcttcgcgc
     4441 tctacggcga caacctggtc ggcttcgtgg acgacggctg ggtgagcctg accgtgaccc
     4501 tggccgtgat cgccggcggg ctcggcttcc cggtgctgat cgagctcatc cgcgaaccgg
     4561 gcaccccgga ccgctggtcg ctgcacacca agctcaccct gacgatgacc tgggtgctgc
     4621 tggcgaccgg gacgctggcc gtcctcgcct tcgagtggtc caacccggcc accttcggcc
     4681 ccctcgacgt ccccggcaag ctgctggccg gcttcttcca gggcgccaac ccgcgctcgg
     4741 ccgggttcaa cacggtcgac tatgccgaga tgggggagac gtcgtggctg gtcaccgagg
     4801 tcctcatgtt catcggggcg ggcagcgccg gcaccaccgg cgggatccgg gtcaccacct
     4861 tcgccgtgct cgtgctgatg gtcgccgccg aggcacgcgg gcgctcaacg gtcaacgcct
     4921 ttgggcgccg gatcccccag ggggcccagc gccaggccct gtcggtcacc ctcatcgggc
     4981 tgggcgcggt ggtcgcggcc accctggcca tcaccgccca gagcgggctc ccgctgtccg
     5041 gcacgctgtt cgaggcgttc tcggccttcg ggaccgtggg gctgacaacc gggatcaccc
     5101 cccagctgcc ggtgccgtcc cagaacctcc tgatcctgct catgttcctg ggccggaccg
     5161 gccccattac actggccacc gcg
//
LOCUS       DASSWR010000208         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1080553, whole genome shotgun sequence.
ACCESSION   DASSWR010000208 DASSWR010000000
VERSION     DASSWR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080553"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..2067
                     /gene="ftsH"
                     /locus_tag="VFO47_03465"
     CDS             145..2067
                     /gene="ftsH"
                     /locus_tag="VFO47_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET8787787.1"
                     /translation="MPNLNRLFRGPMAYLLLILVLVFLFFRFFAASPQVEQARLSDVV
                     SAIGDKRVKDAVLKDQDQKITGTYNDGRKFEAAYTEQQGKDLFNRLQEKGIPARVDPR
                     GQSVLVQILIQLLPFAVLLLLFFFLMQQMQGGGNRVMSFGKSKARLVTKDQPKITFQD
                     VAGVDEAVEELQEIKEFLENPAKFQAMGAKIPKGVLLFGPPGTGKTLLARAVAGEAGV
                     PFFSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVGRQRGAGLGGGHD
                     EREQTLNQLLVEMDGFDVKSGVILIAATNRPDILDPALLRPGRFDRQITVDRPDLKGR
                     LDILKVHARGKPIAPGIDLEVLARRTPGFTGADLANVVNEAALLSARHGRKEIADDEL
                     EEAIDRVIAGPERKTRVISDKEKRMIAYHESGHAIVGHALPNADPIHKVSVISRGRAL
                     GWTLSLPVEDKFLVTRSEMIDELAMLLGGRVAEELWFLDPTTGASNDIEKATQVARKM
                     VCEYGMSDRLGSLVLGTKQENVFLGRDFGHQQDYSEQVAFEIDKEMRRLIDEAHAEAE
                     QILTTYRDVADAMVEALMEKETIGKEELAQILAPVVKRPARGIMAPPPGHVRSNGGRS
                     RDEAGIARAQDGSPSD"
     gene            2064..2660
                     /gene="folE"
                     /locus_tag="VFO47_03470"
     CDS             2064..2660
                     /gene="folE"
                     /locus_tag="VFO47_03470"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET8787788.1"
                     /translation="MSALPGGGFDQDKVERGVRLLLEGIGEDLDRDGLRETPARVARM
                     YAEITSGLREDPTQVLQAVFEADHDEMIMVRDIPMASICEHHLIPFVGKAHVAYIPNT
                     EGRITGLSKLARLVDGLSRRPQVQERLTAQIADAMVERLEPRGALVVIEAEHLCMSMR
                     GVRKPGAITVTSAVRGSFRDSMSTRMEAMNLLGVTRLG"
     gene            2802..>3156
                     /locus_tag="VFO47_03475"
     CDS             2802..>3156
                     /locus_tag="VFO47_03475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET8787789.1"
                     /translation="MGVVNVTPDSFSDGGRYLDPAAAVAHGLALAAEGADLLDVGGES
                     TRPGAADVPEAVELDRVLPVIEELAAKAGVPVSVDTRKAAVAAAAVAAGAGVINDVSA
                     GRHDPELLAVAAEAKV"
BASE COUNT          490 a         1169 c         1054 g          443 t
ORIGIN      
        1 ccggtgaggc gcctgctacg atgacccttc tgaccactcc tttctcttac cgcccctccg
       61 atctggtcgt ggcccgattt cttcgacttc acaaacgcca aggtcgtgcc actatggtcc
      121 ggggaggagc gacaggatga cacgatgccc aatctgaacc gcctgttccg gggaccgatg
      181 gcgtacctgc tgttgatcct cgtcctggtc ttcctcttct tccggttctt cgccgccagc
      241 ccccaggtcg agcaggcccg gttgtcggac gtggtctcgg ccatcggcga caagcgggtc
      301 aaggacgcgg tcctcaagga ccaggaccag aagatcacgg gcacctacaa cgacgggcgc
      361 aagttcgagg ccgcctacac cgagcagcag ggcaaggacc tgttcaaccg gctccaggag
      421 aagggcatcc cggcccgggt cgacccccgc ggccagtcgg tgctcgtcca gatcctcatc
      481 cagctgctcc ccttcgccgt cctcctgctg ctgttcttct tcctcatgca gcagatgcag
      541 ggcggcggca accgggtgat gagcttcggc aagtccaagg cccgactggt caccaaggac
      601 cagcccaaga tcaccttcca ggacgtggcc ggggtcgacg aggcggtcga ggagctccag
      661 gagatcaagg agttcctcga gaacccggcc aagttccagg ccatgggggc caagatcccc
      721 aagggcgtgc tgctgttcgg cccccccggg acgggcaaga ccctgctcgc ccgggccgtg
      781 gccggcgagg ccggggtgcc gttcttctcc atctccgggt ccgacttcgt ggagatgttc
      841 gtcggcgtcg gcgcctcccg cgtccgcgac ctgttcgagc aggccaaggc gaacgccccc
      901 gcgatcgtgt tcgtggacga gatcgacgcc gtcggccgcc agcgcggcgc cggcctcggc
      961 ggcggccacg acgagcgcga gcagaccctg aaccagctgc tggtcgagat ggacggcttc
     1021 gacgtcaagt cgggcgtgat cctgatcgcg gccaccaacc ggcccgacat tctcgacccg
     1081 gccctgctcc gccctggccg cttcgaccgc cagatcaccg tcgaccgccc cgacctcaag
     1141 ggccgcctcg acatcctcaa ggtccacgcc cgcggcaagc cgatcgcccc cgggatcgac
     1201 ctggaggtcc tggcccgccg cacccccggg ttcaccggcg ccgacctggc caacgtggtc
     1261 aacgaggccg ccctgctctc ggcccgccac gggcgcaagg agatcgccga cgacgagctc
     1321 gaggaggcca tcgaccgggt catcgccggc ccggagcgca agacccgcgt gatctccgac
     1381 aaggagaagc gcatgatcgc ctaccacgag tcgggccacg cgatcgtcgg gcacgccctg
     1441 cccaacgccg acccgatcca caaggtgtcg gtgatctccc gcggccgcgc cctcggctgg
     1501 accctcagcc tccccgtcga ggacaagttc ctggtcaccc ggagcgagat gatcgacgag
     1561 ctggccatgc tgctcggcgg ccgggtggcc gaggagctgt ggttcctcga ccccaccacc
     1621 ggcgcctcca acgacatcga gaaggccacc caggtcgccc gcaagatggt ctgcgagtac
     1681 ggcatgagcg accgcctggg ctcgctggtg ctcggcacca agcaggagaa cgtgttcctg
     1741 ggccgcgact tcggccacca gcaggactac tccgagcagg tcgccttcga gatcgacaag
     1801 gagatgcggc ggctgatcga cgaggcccac gccgaggccg agcagatcct gaccacctac
     1861 cgcgacgtgg ccgacgccat ggtcgaggcg ctgatggaga aggagaccat cggcaaggag
     1921 gagctggccc agatcctcgc cccggtggtc aagcgcccgg cccgcggcat catggcccca
     1981 cccccgggcc acgtccgctc caacggcggc cggtcccgcg acgaggccgg catcgcccgc
     2041 gcccaggacg gctcgccgtc cgactgagcg cgctgcccgg cggcggcttc gaccaggaca
     2101 aggtcgaacg cggcgtccgc ctgctcctgg aggggatcgg cgaggacctc gaccgcgacg
     2161 gcctgcgcga gaccccggcc cgggtcgccc gcatgtacgc cgagatcacc tcgggcctgc
     2221 gcgaggaccc cacccaggtc ctgcaggcgg tgttcgaggc cgatcacgac gagatgatca
     2281 tggtccgcga catccccatg gcctcgatct gcgagcacca tctgatcccg ttcgtcggca
     2341 aggcccacgt cgcctacatc cccaacaccg agggccggat caccgggctg tcgaagctgg
     2401 cccggctggt cgacggcctg tccaggcggc cccaggtcca ggagcggctg accgcccaga
     2461 tcgccgacgc catggtggag cggctggagc cgcggggcgc cctggtggtg atcgaggccg
     2521 agcacctctg catgagcatg cgcggggtcc gcaagccggg cgcgatcacc gtgacctcgg
     2581 ccgtccgggg ctccttccgc gactccatgt ccacccgcat ggaggcgatg aacctgctcg
     2641 gggtgacccg cctgggctga cccgggactc ggtaggctca gtaagtaaga tgaccgggca
     2701 tccgccccag ggaggtccgc gtcttgtccg tagcaccgct cgccccgcac gacctgcggg
     2761 tcggtgaccg ggtcctgccg acgtcgacgc gctgcctggt catgggggtg gtgaacgtca
     2821 ccccggactc gttctcggac gggggccgct acctggaccc ggcggcggcc gtggcccacg
     2881 ggctggccct ggccgccgag ggcgccgacc tgctcgacgt cggcggcgag tcgacccgcc
     2941 ccggcgccgc cgacgtgccc gaggcggtcg agctcgaccg ggtcctgccg gtgatcgagg
     3001 agctggccgc caaggccggc gtgcccgtct cggtcgacac ccgcaaggcc gccgtggccg
     3061 cggccgccgt ggccgccggg gccggcgtca tcaacgacgt cagcgccggc cgccacgacc
     3121 ccgagctgct cgcggtggcc gccgaggcca aggtcc
//
LOCUS       DASSWR010000209         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1364583, whole genome shotgun sequence.
ACCESSION   DASSWR010000209 DASSWR010000000
VERSION     DASSWR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364583"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFO47_03480"
     CDS             <1..279
                     /locus_tag="VFO47_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8787790.1"
                     /translation="DGDGRAVRFLEWTWDPDPLDTTYTVDYAYLLREADGSVRVEHDR
                     HVEGLFPRATWLRLLSEAGFQARAVPVEHSELEPGSYELFVATRPHPA"
     gene            complement(276..1475)
                     /locus_tag="VFO47_03485"
     CDS             complement(276..1475)
                     /locus_tag="VFO47_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8787791.1"
                     /translation="MRKVLIAGGGIAGPVTAMALQRAGIDAVVYEAHVPGGDEAGSYL
                     TVATNGLDALRAIDADGPVLDAGFPTPTNVLLSGGGRRLGTVSNGGRLADGTVSHTVK
                     RARLYRALHDQAAGRGVRIEYGRRLAGAEATAGGGVAVTFDDGTRATGDLLVGADGVH
                     SVTRKLIDPAAPDGRYVGLVNFGGYTPDSAGVAEPGSWHMIFGRRAFFGYVADPGGGT
                     VWFANVPRPAVSPAERAATSPDQWRRQLVELFAGDHGPAAGLIADGRLELAGDSTHDL
                     PTVPTWHRGPMVIVGDAAHAPSPTSGQGASMAAEDAVVLAKSLRDRPTVPEALAAYEG
                     LRRRRVERIVAQGARTGSAKTPGRVGRVLRDLSLPLVFKLLVTEQSMAWIYDHHIDWD
                     APVAVPA"
     gene            1539..2021
                     /locus_tag="VFO47_03490"
     CDS             1539..2021
                     /locus_tag="VFO47_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8787792.1"
                     /translation="MTRPLDRRAELLERLALAGRASSAATVMFHTAVAARQGLSASEE
                     KALDLLERSGPLTAGELARRSGLAPASVTGLVDRLERKGFARRVPNPGDGRSVLVEVD
                     SERVYATVAPLFADWVGSLYELYAGYTDEQLEVILHFLTEAARRQQEATARLTADQDP
                     "
     gene            2018..>2651
                     /locus_tag="VFO47_03495"
     CDS             2018..>2651
                     /locus_tag="VFO47_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787793.1"
                     /translation="MRRPTTVGVLVLAAAVGFLLSLYGPSIPELRAAYGVGGGGSALV
                     LSLHFAGAMTGIGWWGLERRLAPRTWLVVAATLAAGGAVGIAFAPAWPVVLAAAFGLG
                     VGFGVVVVEINVLFAEAFGERTTAMLNLLGACFGAGAILGPLAVAASGGYRLPFCVGA
                     GAALASLALAGDLPRTARPPAPAGDRPPFAVVWGFVALCALYVGVENGIGG"
BASE COUNT          287 a          972 c         1033 g          359 t
ORIGIN      
        1 gacggcgacg gccgcgccgt ccgcttcctc gagtggacct gggaccccga cccgctcgac
       61 accacctaca ccgtcgacta cgcctacctg ctgcgcgagg ccgacggctc ggtcagggtc
      121 gagcacgacc gccacgtcga gggcctgttc ccccgggcca cctggctgcg cctgctgtcc
      181 gaggccggct tccaggcccg ggcggtcccg gtggagcact cggaactgga gccaggcagc
      241 tacgagctgt tcgtggccac ccggccgcac cccgcctagg ccgggacggc cacgggggcg
      301 tcccagtcga tgtggtggtc gtagatccag gccatcgact gctcggtgac gagcagcttg
      361 aacaccaggg gcagggacag gtcccggagc acgcggccga cccggccggg ggtcttggcg
      421 ctgcccgtgc gggccccctg ggcgacgatg cgctcgaccc gccggcggcg caggccctcg
      481 taggcggcca gcgcctcggg gacggtcggc cggtcgcgca ggctcttggc gagcacgacc
      541 gcgtcctcgg cggccatgga ggcgccctgg cccgaggtgg gcgagggggc gtgggcggcg
      601 tcgccgacga tcaccatcgg gccccggtgc caggtcggca ccgtgggcag gtcgtgggtg
      661 ctgtcgccgg ccagctcgag gcggccgtcg gcgatcaggc cggcggccgg gccgtggtcg
      721 ccggcgaaca gctccaccag ctggcgccgc cactggtcgg ggctggtggc cgcccgctcg
      781 gccgggctca ccgccggacg ggggacgttg gcgaaccaga ccgtcccgcc gccggggtcg
      841 gcgacatagc cgaagaacgc ccgccggccg aagatcatgt gccagctgcc cggctcggca
      901 acgccggcgc tgtccggggt gtagccgccg aagttgacca ggccgacgta gcggccgtcg
      961 ggggcggcgg ggtcgatcag cttgcgggtc accgagtgga cgccgtcggc gccgaccagc
     1021 aggtccccgg tggcccgggt gccgtcgtcg aaggtgacgg ccacgccgcc gcccgccgtg
     1081 gcctcggccc cggccagccg gcggccgtac tcgatgcgga cgccgcggcc ggccgcctgg
     1141 tcgtgcagcg cccggtacag gcgggcccgc ttgacggtgt ggctcacggt cccgtcggcc
     1201 agccgcccgc cgttgctgac ggtccccagc cgccgcccgc cgccgctgag gagcacgttg
     1261 gtcggggtcg ggaagcccgc gtcgagcacc ggcccgtcgg cgtcgatggc ccgcagggcg
     1321 tccaggccgt tggtggcgac ggtgaggtag gacccggcct cgtcgccgcc ggggacgtgg
     1381 gcctcgtaga cgacggcgtc gatgccggcg cgctgcagcg ccatcgccgt gactgggccg
     1441 gcgatgccgc cgccggcgat caggaccttg cgcatgttcc cgctccctcg tgcagatatt
     1501 tagtttggac aaacgaaata tacgctacac tgtggagtat gacaagaccc ctcgaccggc
     1561 gggcggagct gctcgagcgg ctggccctgg ccggccgggc ctccagcgcg gccacggtga
     1621 tgttccacac cgccgtcgcg gcccggcagg gcctcagcgc cagcgaggag aaggcgctgg
     1681 acctgctgga gcgctccggg ccgctcaccg cgggtgagct ggcccggcgc tcggggctgg
     1741 ccccggcgtc ggtgaccggg ctggtcgacc gcctggagcg caagggcttc gcccggcgcg
     1801 tccccaaccc cggcgacggc cgcagcgtcc tggtcgaggt cgacagcgag cgcgtctacg
     1861 ccaccgtcgc ccccctgttc gccgactggg tggggtcgct gtacgagctg tacgccggct
     1921 acaccgacga gcagctcgag gtgatcctcc acttcctcac cgaggccgcc cgccgtcagc
     1981 aggaggcgac ggcgcggctc accgccgacc aggatccatg aggcgcccca ccaccgttgg
     2041 ggtgctggtg ctggcggcgg cggtcgggtt cctgctgtcg ctgtacgggc cgtccatccc
     2101 cgagcttcgg gcggcctacg gggtgggcgg cggcgggtcg gcgctggtgc tcagcctcca
     2161 cttcgccggg gccatgaccg ggatcggctg gtgggggctg gagcggcgcc tggccccccg
     2221 gacctggctg gtggtcgccg ccaccctggc ggccggcggg gcggtcggga tcgccttcgc
     2281 ccccgcctgg ccggtggtgc tggcggcggc gttcgggctc ggggtcggct tcggggtggt
     2341 ggtggtcgag atcaacgtgc tgttcgccga ggccttcggc gagcggacca cggccatgct
     2401 caacctgctc ggcgcctgct tcggggccgg tgcgatcctc gggccgctgg ccgtcgccgc
     2461 cagcggcggg taccggctgc cgttctgcgt cggcgccggc gcggccctgg cctccctggc
     2521 cctggccggc gacctgccga ggacggcccg gccgcccgcc cccgccgggg accggccgcc
     2581 gttcgcggtg gtctgggggt tcgtcgccct gtgcgccctg tacgtcgggg tggagaacgg
     2641 gatcggcggc t
//
LOCUS       DASSWR010000210         4241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_856270,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000210 DASSWR010000000
VERSION     DASSWR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856270"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..896
                     /locus_tag="VFO47_03500"
     CDS             126..896
                     /locus_tag="VFO47_03500"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HET8787794.1"
                     /translation="MNFASNTALYANPDLAEGVDWARRFRRHELFDDSLARTGEVAHT
                     AIIAPHGGGIEPGTSELCLAVAGYHPATLPQTPPAGVTYDYWMFEGLRARDNDKLHVT
                     STGCDDEIALSLCGGALHGVALHGFLPDPGDDTDVLVGGATEGVDGSLRQSMLDALNE
                     AGFKAVDANGDGELDGDSECNIVNRTLLKRGVQLELSKPLRESMFGEFSRSRRKHTTN
                     QVFWTFVAVCRDVLDRVEADRLVPPLCQPEPQPTLTMS"
     gene            complement(872..1822)
                     /locus_tag="VFO47_03505"
     CDS             complement(872..1822)
                     /locus_tag="VFO47_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET8787795.1"
                     /translation="MNSRVTHLRRLLVLLVLAAVAAVPATASAGTFPDTIRLPDGWQP
                     EGIASGRGTSLYVGSIPTGAVWKGDARTGEGDVLVPPRPGERSAIGIKVDKRNRLFVA
                     GGATGKAFVYDARTGEDLASYQLAPAGTAVTFVNDVVVTSKAAWFTDSRNQQLYALPL
                     GRHGALPDQDDVRVLPLTGELEYTPATTDVDLNGIVAAEGGRVLLSVQSSTGKLFRIN
                     PRTGKAREVDLGGATLTNGDGLLLAGRVLFVVQNRLNQIAVVVLSRSLDRGRVVTTIA
                     DPDFDVPTTIAFQAGRLYAVNARFGTTDPQPARYDIVKVG"
     gene            2028..>4241
                     /locus_tag="VFO47_03510"
     CDS             2028..>4241
                     /locus_tag="VFO47_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787796.1"
                     /translation="MPSASLRSTRSLAVIAALAIVLATLAAGLVAARPADAAQPAPGH
                     TGLVPSTPRTNTPRISSGEIWDIEVVGQRVFIAGTFTSLRNNTATNTTSVSQRFLASY
                     NWSTGLIDTSFRPTFGGGGVNAVEASPDGTRLYVAGSFNTVNGVTKRKIASLNLTTGA
                     PIAGFTANAGAQATALAATNTTLYVGGNFKTVNGAARVGLVAVNGTTGAVVPGFVNNM
                     SGGIGVNGLLTVQQLKLTHDDTKLLVVHTGRKIADQDRYGVGLISTQTNQLLPWRTRL
                     WDDNLQFVGGIQRVYAGDIAPNDQYFVVGSGSGGDRPPINDTAIAFPVAGNDNVQPLW
                     VSRHFDSVYSVAITERAVYVGGHFGFQESPTAPDPWPGLDNVGYGTGQGLAGYGLGDA
                     VVRRDHIGALNPADGKALEWNPGSNSFEGNKAMEATPRGLFVGGDGQYQGGKLTGRVA
                     FYDFNTAPAATAVDTTITTPIEGRVVQSGVQFTIQGRATATGGQVRRVQVEIQDRNTK
                     QYLQDNLTTWGGVNNIYASLDTPNAASTAWSLPVTITGNRELQVMAKTFAVGGSSDPS
                     KAIKKIESFGIDDQTPTTSINGPSGSVLASTTFTATGTASDDKGVNALTFWFRNASNQ
                     YLQEDGSVSSNFNTFRGTPDVVGATSATWQYEVTLPHEGEWRMSATAIDNAGQSDLRS
                     ATRDWLITSTGVAPAVAINAPAPMTPPTAAPTYTVTPGGRLTFSGTANDTDDLVNI"
BASE COUNT          678 a         1592 c         1410 g          561 t
ORIGIN      
        1 gttcgctccg ctccttcgcc gaggctgatc gttcgcctca ccgacggaga atccaccagg
       61 gccgtccccg attcccaccg gctggccgtt cccatcgggt ccggatcgtc gtatggtcgc
      121 gagcgatgaa ctttgcctcg aacaccgcac tctacgcgaa ccccgacctc gcggaggggg
      181 tggactgggc gcgccggttc cgccgccacg agctgttcga cgacagcctg gcccgcaccg
      241 gcgaggttgc ccacaccgcg atcatcgccc cgcacggggg cgggatcgag ccgggcacgt
      301 ccgagctctg cctggccgtc gccggctacc acccggcgac ccttccccag acgccgcccg
      361 ccggggtcac ctacgactac tggatgttcg aggggctgcg ggcgagggac aacgacaagc
      421 tccacgtcac ctccaccggg tgcgacgacg agatcgccct ctcgctgtgc gggggggcgc
      481 tgcacggggt ggccctgcac ggcttcctcc ccgaccccgg ggacgacacg gacgtgctcg
      541 tcgggggcgc cacggagggc gtcgacgggt ctctgcggca gtccatgctg gacgcgctga
      601 acgaggccgg gttcaaggcg gtcgacgcca acggggacgg ggagctcgac ggcgacagcg
      661 agtgcaacat cgtcaaccgc accctgctca agcggggcgt tcagctggag ctcagcaaac
      721 cgctgcggga gtcgatgttc ggcgagttca gccgctcgcg ccgcaagcac accaccaacc
      781 aggtgttctg gaccttcgtg gcggtgtgcc gcgacgtgct cgaccgggtc gaggcggacc
      841 ggctcgtccc gcctctctgc cagccggaac ctcagccgac cttgacgatg tcgtagcggg
      901 cgggctgcgg gtcggtggtg ccgaagcggg cgttgaccgc gtacaggcgg ccggcctgga
      961 aggcgatcgt ggtcggaacg tcgaagtcgg ggtcggcgat ggtggtcacc acccggcccc
     1021 ggtcgagcga ccgggacagc acgaccacgg cgatctggtt gagccggttc tggaccacga
     1081 acagcacccg tccggccagc agcaggccgt cgccgttggt cagggtggcc ccgccgaggt
     1141 cgacctcgcg ggccttgccg gtgcgggggt tgatgcggaa cagcttgccg gtgctggact
     1201 ggaccgacag cagcacccgg ccgccctcgg cggccacgat tccgttgagg tcgacgtcgg
     1261 tggtggccgg cgtgtactcc agctcgccgg tgagcggcag gacgcgcacg tcgtcctggt
     1321 cgggcagggc gccgtggcgg ccgagcggca gggcgtacag ctgctggttg cgcgagtcgg
     1381 tgaaccaggc ggctttggag gtcacgacca cgtcgttgac gaaggtcacg gccgtgccgg
     1441 ccggggcgag ctggtagctg gccagatcct cgccggtcct ggcgtcgtag acgaacgcct
     1501 tgccggtggc cccgccggcc acgaacaggc ggttgcgctt gtcgaccttg atgccgatgg
     1561 cgctgcgctc ccccggccgg ggcgggacca gcacgtcgcc ctcgccggtc ctggcgtcgc
     1621 ccttccagac ggccccggtc gggatcgacc cgacgtacag ggaggtgccg cggccgctgg
     1681 cgatcccctc gggctgccag ccgtcgggca ggcggatggt gtcggggaag gtgcccgcgg
     1741 aggcggtcgc cggcacggcc gcgaccgcgg ccaggaccag caggacgagc aggcggcgta
     1801 ggtgcgtgac cctggagttc atcggccctc ccagtcgcgg cggcgcgaac ggcccaaatc
     1861 cggttgagcc tgcaaccacc ggcataagga ggagataagc agacagggca agcatgcccc
     1921 ccgccgatgc ctgaccccgg ctacgtccga tgcatggttg ccaggtccag cgtgctgcct
     1981 agcatccgct gaactgccag cagtccgacc gttcaccggg agaccacgtg ccaagcgcca
     2041 gcctccgcag cacccgatcc cttgccgtca tcgccgcgtt ggcgatcgtc ctcgcgaccc
     2101 tcgccgccgg cctggtggcg gcgaggcccg cggacgcggc gcagcccgct cccgggcaca
     2161 cgggcctggt gcccagcacg ccaaggacca acaccccccg gatcagcagc ggcgagatct
     2221 gggacatcga ggtggtgggc cagcgcgtct tcatcgcggg caccttcacc tcgctgcgga
     2281 acaacaccgc gaccaacacg acatcggtca gccagcgctt cctggcctcc tacaactgga
     2341 gcaccgggct gatcgacacc tcgttccggc ccaccttcgg cggcggcggc gtcaatgccg
     2401 tcgaggcctc gcccgacggc accaggctgt acgtggccgg ctccttcaac accgtcaacg
     2461 gcgtcaccaa gcgcaagatc gcctcgctca acctgaccac cggcgccccg atcgccggct
     2521 tcaccgccaa cgccggcgcc caggccaccg ccctcgccgc caccaacacc accctgtacg
     2581 ttggcggcaa cttcaagacc gtgaacggcg ccgcccgggt cggcctggtg gcggtcaacg
     2641 gcaccaccgg cgcggtcgtc cccggcttcg tcaacaacat gtccggcggc atcggcgtca
     2701 acggcctgct caccgtccag cagctcaagc tgacccacga cgacaccaag ctcctggtcg
     2761 tccacaccgg ccgcaagatc gccgaccagg accgctacgg cgtcggcctg atcagcaccc
     2821 agaccaacca gctgctcccc tggcgcaccc gcctgtggga cgacaacctc cagttcgtcg
     2881 gcggcatcca gcgcgtctac gccggcgaca tcgcccccaa cgaccagtac ttcgtggtcg
     2941 gcagcggctc cggcggcgac cgcccgccga tcaacgacac cgccatcgcc ttcccggtcg
     3001 ccggcaacga caacgtccag ccgctgtggg tctcgcgcca cttcgacagc gtctactcgg
     3061 tggccatcac cgagcgggcg gtgtacgtcg gcggccactt cggcttccag gagtcgccca
     3121 ccgccccgga cccctggccc ggcctggaca acgttggcta cggcaccggg cagggcctgg
     3181 ccggctacgg cctcggcgac gcggtggtcc gacgcgacca catcggcgcc ctcaacccgg
     3241 ccgacggcaa ggcgctggag tggaaccccg gctccaacag cttcgagggc aacaaggcca
     3301 tggaggccac cccccgtggc ctgttcgtcg gcggcgacgg ccagtaccag ggcggcaagc
     3361 tgaccggccg ggtcgccttc tacgacttca acaccgcccc cgcggccacg gcggtcgaca
     3421 ccaccatcac caccccgatc gagggccggg tggtccagtc cggcgtccag ttcaccatcc
     3481 agggccgcgc caccgcgacc ggcgggcagg tgcgccgggt ccaggtcgag atccaggacc
     3541 gcaacaccaa gcagtacctc caggacaacc tgaccacctg gggcggcgtg aacaacatct
     3601 acgcgtcgct cgacaccccg aacgcggcct cgaccgcctg gtcgctgccg gtcaccatca
     3661 ccggcaaccg tgagctgcag gtgatggcca agaccttcgc cgtcggcggc agcagcgatc
     3721 cctccaaggc gatcaagaag atcgagtcct tcggcatcga cgaccagacc ccgacgacca
     3781 gcatcaacgg gcccagcggc agcgtgctgg ccagcaccac cttcaccgcc accgggacgg
     3841 ccagcgacga caagggggtc aacgccctca ccttctggtt ccgcaacgcg agcaaccagt
     3901 acctgcagga ggacgggtcg gtctcgtcca acttcaacac cttccggggc acccccgacg
     3961 tggtcggcgc gacctcggcc acctggcagt acgaggtgac cctgccgcac gagggcgagt
     4021 ggcgcatgtc ggccacggcc atcgacaacg ccggccagtc ggacctccgc agcgcgaccc
     4081 gcgactggct gatcacctcc accggggtgg cgccggcggt ggcgatcaac gccccggcgc
     4141 cgatgacgcc gccgaccgcg gcgcccacct atacggtgac cccgggcggg cggctgacct
     4201 tcagcggcac cgccaacgac accgacgacc tggtcaacat c
//
LOCUS       DASSWR010000211         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_517079,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000211 DASSWR010000000
VERSION     DASSWR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517079"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFO47_03515"
     CDS             complement(<1..328)
                     /locus_tag="VFO47_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787797.1"
                     /translation="MAELRTPAAGSAGDQPEALLAEYEAEKPARHLTGPARLVVSAAG
                     VGLSCYAIWWVLDPQPAQWYRMTFLAVALGMTFLVYRPWTRRRAGGAPAGEPGPDERP
                     ERPGNGQ"
     gene            complement(591..1688)
                     /locus_tag="VFO47_03520"
     CDS             complement(591..1688)
                     /locus_tag="VFO47_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8787798.1"
                     /translation="MGRLTGIDTVLLLDLDGTLYVGSQVVPGAPETVRWAREGETPMA
                     TDNAINSFTLDIDQLGWTGQDLHRPESVVAEPDGTLWTSDGRGGVTRIDPDGGQTLLG
                     GWGGEPNGLAIDPNGNLVTANIALGRVQQMTRDGQISTVLEEVDGQRTTSANFVFYDR
                     SGRLWLTCLTREDHWWPAVAHPRPDGFIVLVDDRGARIVADGIYATNEARLDAEERYL
                     YVAETMKARILRFPVRSDGSLGEREVFGPDGLGLGGFVDGFTFDAEGNLWVTTVVRNG
                     IGVLTRDGDWHVVLEDPREDVLAGFVDKLAAGAATPEDMLAAAGPRLQFPTSVCFAGP
                     DLRTAYVGSLAMSRLPTFRAPVPGLPMSHWS"
     gene            complement(1849..2238)
                     /locus_tag="VFO47_03525"
     CDS             complement(1849..2238)
                     /locus_tag="VFO47_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787799.1"
                     /translation="MTAETWWVAGTAAAVVLVPCLAIQLRRSWAGLVARRQARLRAEA
                     LLWAWLSPAQRKQYRARRWFEVTTPSGRRYRILRGGVVRIDRHGSGYCIEATSPVPVA
                     DEMLANKLLLETDERRFLATAHRFLYR"
     gene            complement(2248..>2503)
                     /locus_tag="VFO47_03530"
     CDS             complement(2248..>2503)
                     /locus_tag="VFO47_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787800.1"
                     /translation="PLEEARQMTGTMHVADHQGDTTHTWDTTDPSTVQEIEAIFREAQ
                     ATGRLVYRQTGDGSGEQVRLATWNPEEHTELFVAPRLAGG"
BASE COUNT          346 a          907 c          885 g          365 t
ORIGIN      
        1 gctgcccgtt cccgggtcgc tcgggccgct cgtccggccc gggttcgccg gccggtgccc
       61 ctcccgcccg ccggcgcgtc cagggccggt agaccaagaa cgtcataccc agggcgacgg
      121 ccaggaaggt catccggtac cactgggcgg gttgcgggtc cagcacccac cagatcgcgt
      181 aacaggacaa cccgactccg gccgccgaca cgaccagcct ggcaggaccg gtcaggtggc
      241 gggccggctt ttctgcctcg tactcggcga gcagcgcctc gggctggtcg ccggccgacc
      301 cagccgcggg tgttcgcagc tcggccatgg gtgatgtctt ccgtcgcctc aggcgcggtc
      361 gttcaggtct tggtgcccag atggtgggcg cataaacccg gggcggccgt cggcggggct
      421 ggagctgccg atcgtcgccg tgctaccaac cgcccggacc ggctatgcgg agctgctgtt
      481 gcagtgcggc cacgcccagc ctggcggttc gggcggatga tctgctcggc cgcgagctgg
      541 cgctggcgat gctggccgag gagggggacc gcgggctccg ctcggatggc tcaggaccag
      601 tggctcatcg gcagacccgg gacgggagcg cggaaggtgg gcagccgcga catggccagc
      661 gagcccacgt acgccgtgcg caggtctggg ccggcgaagc acacgctggt cgggaactgc
      721 agcctcggcc cggcggcggc cagcatgtcc tcgggtgtgg ccgcgcccgc cgccagcttg
      781 tcgacgaagc cggccagcac gtcctcgcgg ggatcctcca ggaccacatg ccagtcgccg
      841 tcgcgggtca gcaccccgat gccgttgcgg accaccgtgg tgacccacag gttgccctcg
      901 gcgtcgaagg tgaacccgtc cacgaacccg ccgaggccca gcccgtcggg gccgaacacc
      961 tcccgttcgc ccagcgaccc gtcggagcgg accgggaacc gcaggatgcg ggccttcatc
     1021 gtctcggcga cgtacaggta ccgctcctcg gcgtccagcc gggcctcgtt ggtggcgtag
     1081 atgccgtcgg ccacgatccg ggcgccgcgg tcgtcgacca gcacgatgaa cccgtccggg
     1141 cggggatggg cgaccgccgg ccaccagtgg tcctcgcggg tcaggcaggt cagccacaac
     1201 cgcccggagc ggtcgtagaa cacgaagttg gcgctggtgg tgcgctgccc gtcgacctcc
     1261 tccagcacgg tggagatctg gccgtcgcgg gtcatctgct gcacccgccc aagggcgatg
     1321 ttggcggtga ccaggttgcc gttggggtcg atggccagcc cgttgggttc cccgccccac
     1381 ccgcccagga gtgtctggcc gccgtcgggg tcgatgcggg tcaccccgcc gcggccgtca
     1441 gaggtccaca gcgtcccgtc cggctcggcg accaccgatt cgggacggtg caggtcctgg
     1501 ccggtccagc ccagctggtc gatgtcgagg gtgaagctgt tgatcgcgtt gtctgtggcc
     1561 attggcgtct ctccctctcg agcccaccgc accgtctcgg gggcgccggg cacgacctgg
     1621 ctgcccacgt acagcgtgcc gtccaggtcc agcagcagca cggtgtcgat gccggtcagc
     1681 cgtcccacga cccacctccg ccacgccgcc tggaccaccc aggcaaggct accggtccgc
     1741 cgtggcgaag tcagaccgac tggatcctcc cgctacggct gatgggcgtt cgctccagac
     1801 cacgctcgag cccgggccac tcgagccctg caatgtcgta ccgcctgttc agcggtagag
     1861 gaagcggtgg gcggtggcca ggaagcggcg ctcgtcggtc tccaagagca gcttgttggc
     1921 cagcatctca tcggccaccg ggaccggcga ggtcgcctcg atgcagtagc cagacccgtg
     1981 ccggtcgatc cgcacgaccc cacctcgcag gatccggtag cgacgaccgg agggggtcgt
     2041 aacctcaaac cagcgccgcg cccggtactg cttccgctgg gcgggcgaca gccaggccca
     2101 cagcaacgcc tcagcccgca gcctggcctg gcgccgggcc acaagcccag cccaggaacg
     2161 gcggagctgg atcgccaggc aggggacgag gacgacggcg gcggccgtcc cggccaccca
     2221 ccaggtctct gcggtcatgc cggaacgcta gccgccagcc agtcgcgggg cgacgaacag
     2281 ctcggtatgt tcctcggggt tccaggtggc caagcgcacc tgttcgccgc tgccgtcccc
     2341 ggtctggcgg tacaccagcc gcccggtcgc ctgcgcctcc cggaagatcg cctcgatctc
     2401 ctggacggtg ctcgggtcgg tggtgtccca ggtgtgggtg gtgtcgccct ggtggtcggc
     2461 gacatgcatg gtgccggtca tctgccgcgc ctcctccaac ggc
//
LOCUS       DASSWR010000212         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1223935, whole genome shotgun sequence.
ACCESSION   DASSWR010000212 DASSWR010000000
VERSION     DASSWR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223935"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFO47_03535"
     CDS             <1..920
                     /locus_tag="VFO47_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009302688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET8787801.1"
                     /translation="FPLGLLWFVVTFTLWTAALGLLTAPLWYWIPADGGVALITDGDR
                     GLLVIDTLPEALLATVVGAGVCVAAAWAVKGMAAAHGAVALALLGPSQHQLQARVDAL
                     QASRDRAVDTAEAERRRIERDLHDGAQQRLVALAMDLGMARAKLESDPAAGAALVGEA
                     HEEAKRALAELRDLARGIHPAVLADRGLDAAISALAARSPVPVGVEVATGRLPGPVES
                     AAYFVVAEALANAAKHAGATEIGVRITRHRDLLVVEVIDDGAGGADPAKGTGLRGLAD
                     RVAAVDGRLTVTSPAGGPTVIRAELPCGS"
     gene            908..1552
                     /locus_tag="VFO47_03540"
     CDS             908..1552
                     /locus_tag="VFO47_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8787802.1"
                     /translation="MRVVIAEDSVLLREGLARLLADGGVEVVAQVGDGPGLVAAVEAY
                     RPDLAVVDVRMPPSHTDEGLRAALEVRERAPETAILVLSQYVEERYAGDLLAGGAQGV
                     GYLLKDRVADVGEFLAAVRRVGDGGAAIDPEVVAQLLGRRRKADPLASLSPRELDVLT
                     LMAEGRSNAAIAERLVITEGAVEKHISSIFAKLGLPAAPTDHRRVLAVLTYLRA"
     gene            1689..2243
                     /gene="pyrR"
                     /locus_tag="VFO47_03545"
     CDS             1689..2243
                     /gene="pyrR"
                     /locus_tag="VFO47_03545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET8787803.1"
                     /translation="MSGPGMVLDAADVHRALVRIAHEIVERNKGTDGLVVVGIHTRGV
                     PLAGRLAAKLAEIEGHQVPAGALDVAMYRDDIGMRPPQATHDTELPFDLAGKVVVLVD
                     DVLYTGRTVRAALDALTDFGRPQAIQLAVLVDRGHRELPLRADYVGKNLPTAPDQAVR
                     VLLAETDGEDGVVIEDTPRKAVAS"
     gene            2240..3166
                     /locus_tag="VFO47_03550"
     CDS             2240..3166
                     /locus_tag="VFO47_03550"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET8787804.1"
                     /translation="MNKHLLSAADLSAGDITRVLDTAAHLAKVTDRAIKKVPTLRGRT
                     VCNLFFEDSTRTRISFELAAKRLSADVINFSAKGSSVSKGESLKDTAITLEAMGVDAI
                     VMRHGASGAPWRLAEWVEAVVVNAGDGTHEHPTQALLDLYTMRQRFPSFDGLKVAIVG
                     DVLHSRVARSNVLALTTMGAEVTLVAPPTLLPARVETWGAAVSYDLDAVLPKQDVVMM
                     LRVQLERQRAGFFPSLREYARLYGLDGRRLDAMADHAIVMHPGPMNRGVEIAAEVADS
                     IERSVIADQVANGISVRMALLYLLLGGPEVAA"
     gene            3163..>3458
                     /locus_tag="VFO47_03555"
     CDS             3163..>3458
                     /locus_tag="VFO47_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8787805.1"
                     /translation="MTRLLLKGGRLVDPASRTDATADLLVVDGTIAEGGPGLDAEGAE
                     VVDADGLVALPGLVDLHTHLREPGREDAETVLTGSRAAAAGGFTAVLAMANTSPV"
BASE COUNT          434 a         1347 c         1242 g          435 t
ORIGIN      
        1 tgttccccct ggggctgctc tggttcgtgg tcaccttcac cctctggacc gccgccctcg
       61 gcctgctcac ggcgccgctg tggtactgga tcccggccga cggcggggtc gccctgatca
      121 ccgacggcga ccgcgggctc ctggtgatcg acaccctgcc cgaggccctc ctggccacgg
      181 tcgtcggggc cggggtgtgc gtggccgcgg cctgggcggt caaggggatg gcggccgccc
      241 acggggccgt cgccctggcc ctgctcgggc ccagccagca ccagctccag gcccgggtcg
      301 acgccctcca ggccagccgc gaccgggccg tcgacacggc cgaggccgag cgccgccgca
      361 tcgagcgcga cctccacgac ggcgcccagc agcggctggt cgccctggcc atggacctcg
      421 gcatggccag ggccaagctg gagagcgacc cggcggccgg ggccgccctg gtcggcgagg
      481 cccacgagga ggccaagcgg gccctggccg agctgcgcga cctggcccgg ggcatccacc
      541 cggccgtgct cgccgaccgg ggcctggacg cggccatctc cgccctggcc gcccgctcgc
      601 ccgtccccgt cggggtcgag gtcgccaccg ggcggctccc cgggccggtc gagtcggccg
      661 cctacttcgt ggtcgccgag gccctcgcca acgcggccaa gcacgccggg gcgaccgaga
      721 tcggcgtgcg catcacccgc caccgggacc tgctggtggt cgaggtcatc gacgacggcg
      781 ccggcggggc cgacccggcc aagggcaccg gcctgcgcgg cctggccgac cgggtggccg
      841 ccgtcgacgg ccgcctgacc gtcaccagcc cggccggagg cccgacggtg atcagggcgg
      901 agctgccgtg cgggtcgtga tcgccgagga ctccgtcctg ctccgcgagg gcctggcccg
      961 gctgctggcc gacggcgggg tcgaggtcgt cgcccaggtc ggcgacggcc ccggtctggt
     1021 cgcggccgtc gaggcctacc gccccgacct ggccgtggtc gacgtccgca tgcctccgag
     1081 ccacaccgac gagggcctcc gggccgccct cgaggtccgc gagcgtgccc ccgagacggc
     1141 catcctggtc ctgtcccagt acgtcgagga gcgctacgcc ggcgacctgc tcgccggcgg
     1201 cgcccagggc gtcggctacc tgctcaagga ccgggtcgcc gacgtcggcg agttcctggc
     1261 cgccgtccgc cgggtcggcg acggcggcgc cgccatcgac cccgaggtgg tcgcccagct
     1321 cctcggccgc cgccgcaagg ccgatcccct ggcctccctc agcccccgcg agctcgacgt
     1381 cctcaccctg atggccgagg gccgcagcaa cgccgccatc gccgagcgcc tggtcatcac
     1441 cgagggcgcc gtcgagaagc acatctccag catcttcgcc aagctcggcc tccccgccgc
     1501 ccccaccgac caccgccggg tcctggccgt cctcacctac ctccgggctt gagcctgccg
     1561 ggctgggcgg ctaccatgcc cggcgacgag cgacctttaa gggccggtcc cgtgaggccg
     1621 ggaagggagc caccgagcat gctgcgctgc gccacgcctc catccccgac gggttggagg
     1681 cgttcttgat gtccgggccg gggatggtgc tggacgccgc cgacgtgcac cgggcgctgg
     1741 tccggatcgc ccacgagatc gtggagcgga acaagggcac cgacgggctg gtggtggtcg
     1801 gcatccacac ccgcggggtg ccgctggccg ggcggctggc ggccaagctg gccgagatcg
     1861 agggccacca ggtcccggcc ggcgccctgg acgtggccat gtaccgcgac gacatcggca
     1921 tgcggccgcc ccaggccacc cacgacaccg agctgccgtt cgacctggcc ggcaaggtgg
     1981 tggtcctggt cgacgacgtg ctctacaccg ggcggacggt acgggccgcc ctggacgccc
     2041 tcaccgactt cgggcggccg caggccatcc agctggccgt gctggtcgac cgcggccacc
     2101 gcgagctgcc cctgcgggcc gactacgtcg gcaagaacct gcccaccgcc cccgaccagg
     2161 cggtccgggt gctgctggcc gagaccgacg gcgaggacgg cgtggtcatc gaggacacgc
     2221 cgaggaaggc ggtggcgtcc tgaacaagca cctgctgtcg gcggccgacc tcagcgccgg
     2281 cgacatcacc cgggtcctgg acacggccgc ccacctggcc aaggtcaccg accgggccat
     2341 caagaaggtc ccgaccctgc gcggccgcac cgtctgcaac ctgttcttcg aggactcgac
     2401 ccggacccgg atctccttcg agctggccgc caagcgcctc tcggccgacg tcatcaactt
     2461 ctcggccaag ggctccagcg tctccaaggg ggagagcctc aaggacacgg ccatcaccct
     2521 ggaggcgatg ggggtggacg ccatcgtgat gcgccacggc gcctcggggg cgccctggcg
     2581 gctggccgag tgggtcgagg cggtggtcgt caacgccggc gacggcaccc acgagcaccc
     2641 cacccaggcc ctgctcgacc tctacaccat gcgccagcgg ttccccagct tcgacgggct
     2701 caaggtggcc atcgtcggcg acgtgctcca ctcccgggtg gcccgctcca acgtgctcgc
     2761 cctcaccacc atgggcgccg aggtcaccct ggtcgccccg ccgaccctgc tcccggcccg
     2821 ggtcgagacc tggggggcgg ccgtctccta cgacctggac gccgtcctgc ccaagcagga
     2881 cgtggtgatg atgctccggg tccagctgga gcggcagcgg gccgggttct tcccctccct
     2941 gcgcgagtac gcgcggctgt acgggctgga cgggcgccgg ctggacgcca tggccgacca
     3001 cgccatcgtc atgcacccgg ggccgatgaa ccgcggggtc gagatcgccg ccgaggtggc
     3061 cgactccatc gagcggtcgg tgatcgccga ccaggtggcc aacggcatca gcgtccgcat
     3121 ggccctgctc tatctcctgc tcggcgggcc ggaggtggcg gcatgaccag gctgctgctc
     3181 aaggggggcc ggctggtcga cccggcctcg cgcaccgacg ccaccgccga cctgctcgtc
     3241 gtcgacggca ccatcgccga gggcgggccc ggtctcgacg ccgagggggc cgaggtggtg
     3301 gacgccgacg gcctggtcgc gctgccgggc ctggtggacc tgcacacgca cctgcgcgag
     3361 ccgggccgcg aggacgccga gaccgtgctg accggctccc gagcagcggc ggccggtggc
     3421 ttcacggcgg tgctggccat ggccaacacc tccccggt
//
LOCUS       DASSWR010000213         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_912042,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000213 DASSWR010000000
VERSION     DASSWR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912042"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1742
                     /locus_tag="VFO47_03560"
     CDS             138..1742
                     /locus_tag="VFO47_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET8787806.1"
                     /translation="MTIESWFLTASERGNRGTDIDRRRGDGRAWTEGNRVEALIHGAS
                     YYPRLLAALRDLGPGDLVGITDWRGDTDELLDGEGTELGKVLAELAGRGVQVRGLLWR
                     SHPDVAHFQEEANLHLGEVVNEAGGEILLDERVRGPGSQHQKLVLIRHPDREDDDVAF
                     MGGIDLCHGRRDDERHLGDPQAVEMDERYGDHPPWHDVQLEVRGPAIGDLSVTFRERW
                     EDPTPLDHRNPWRARLTRRVDQPRHPSPLPPMLRDPAPAGPHAVQVLRTYPAKRPALP
                     FAPSGERSIARAYLKALWRARRLIYLEDQYLWSEDIAQALADALRRSPDLRLIAVVPR
                     YPDQDGRMSGPPNRIGQQAALDAVREAGGDRVAVYDLVNEHGTPIYVHAKVCVIDDVW
                     AAVGSDNLNRRSWTHDSELSAAVLDETLDEREPRDPAGLGDGARTFARQLRLQLWREH
                     LGRGPDDDGDLLDPVEGFDAWRRAAAALAAWHEAGRRGPRPPGQAIEHDPGRVQPWAA
                     WWALPVYKLLVDPDGRPRRLRRAGRF"
     gene            complement(1739..2395)
                     /locus_tag="VFO47_03565"
     CDS             complement(1739..2395)
                     /locus_tag="VFO47_03565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8787807.1"
                     /translation="MHGPFELVIFDCDGVLVDSERLSIRVETEYLAELGWPLTEAEIV
                     ERFMGQSDEYMDEAIEAQLGSRLPGDWKEQFQRRYREAFAASLVPVDGVVEALDQITA
                     ATCVASSGSHDKLRFTLSRTGLYERFEGRIFSGHEVANNKPAPDLFLHAAARMGAEPA
                     RCAVVEDTLYGLMGARAAGMRAFGYAGGLSSPDRLAGKATVVFHDMRDLPRLLADGRG
                     "
     gene            complement(2401..3432)
                     /locus_tag="VFO47_03570"
     CDS             complement(2401..3432)
                     /locus_tag="VFO47_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET8787808.1"
                     /translation="MFHVKPWSVEPRGRFEQQTIRSQVLEGNPLGDPAERPLWVYLPP
                     GIGDDPGARYPSIYVIQGYTGQVDMWWNRTALRPTVPELVDELFADPASPPAIVVFVD
                     CWTSLGGSQFLNSPGTGRYLDYLCDEVVAFVDGRYPTLPAREHRGIAGKSSGGYGAMV
                     TPMLRPDVFGALASHAGDALFEHCYLPDISHAARALRDSYGGSYERFLADFAGRPAFS
                     KEDDGALMNMWAMAACYSAEPDGTVTLPFDPATGRLRDEVWARWLALDPVRMAAGHAA
                     ELRSLRGVYLDAGRRDEYWLDLGAAAFSAELDALGVDHVFELFDATHAAIEYRYPKGL
                     AFLADRLGS"
BASE COUNT          486 a         1259 c         1312 g          423 t
ORIGIN      
        1 acgctccttg cggtcgggaa ggggcaaggg agagcatggc acaggcgctc ttggggggcc
       61 gtggggggga gcgagctccc cccacatgga tcagggactg cggcgggagg cgaatcggtg
      121 gagagctggc gtcgccgatg accatcgaga gctggttcct caccgcttcg gagcgtggca
      181 accggggcac cgacatcgac cggcgccggg gggacgggcg ggcctggacc gaggggaacc
      241 gggtcgaggc gctgatccat ggggccagct actacccgcg gctgctggcc gccctgcgcg
      301 acctcggccc tggcgacctg gtcggcatca ccgactggcg cggcgacacc gacgagctcc
      361 tggacgggga gggtaccgag ctcggcaagg tcctggccga gctggccggc cggggggtgc
      421 aggtccgcgg cctgctgtgg cgctcccacc cggacgtggc ccacttccag gaggaggcga
      481 acctgcacct gggcgaggtg gtgaacgagg ccgggggcga gatcctgctc gacgagcggg
      541 tccggggccc gggcagccag caccagaagc tggtcctgat ccgccacccc gaccgggagg
      601 acgacgacgt cgccttcatg ggcgggatcg acctgtgcca cggtcgccgg gacgacgagc
      661 gccacctcgg cgacccccag gccgtcgaga tggacgagcg ctacggcgac cacccgccct
      721 ggcacgacgt gcagctggag gttcggggcc cggccatcgg cgacctgtcg gtcaccttcc
      781 gggaacggtg ggaggacccg accccgctcg accaccgcaa cccgtggcgg gcccggctga
      841 ccaggcgggt cgaccagccg cggcacccca gcccgctgcc gccgatgctg cgcgacccgg
      901 ccccggccgg cccccacgcc gtccaggtgc tgcggaccta cccggccaag cggccggcgc
      961 tgccgttcgc cccgtccggg gagcgcagca tcgcccgcgc ctacctcaag gccctgtggc
     1021 gggcccgccg gctcatctac ctcgaagacc agtacctgtg gtcggaggac atcgcccagg
     1081 cgctggccga cgcgctgcgc cgctcgcccg acctgcggct gatcgccgtc gtcccccgct
     1141 acccggacca ggacgggcgc atgagcggcc cgcccaaccg catcggccag caggccgccc
     1201 tggacgcggt ccgggaggcc ggcggcgacc gggtcgccgt ctacgatctc gtgaacgagc
     1261 acggcacccc gatctacgtg cacgccaagg tgtgcgtgat cgacgacgtg tgggcggcgg
     1321 tcggctccga caacctgaac cgccgctcct ggacccacga cagcgagctg tcggcggcgg
     1381 tgctcgacga gaccctggac gagcgcgagc cgcgcgaccc cgccggcctc ggggacggcg
     1441 cccggacgtt cgcccggcag ttgcggctgc aactgtggcg cgagcacctc gggcggggcc
     1501 cggacgacga cggcgacctg ctcgacccgg tcgaaggctt cgacgcctgg cgccgggcgg
     1561 ccgcggccct ggccgcctgg cacgaggccg gccgccgcgg cccccgccca cccggccagg
     1621 ccatcgagca cgacccgggc cgggtccagc cctgggccgc ctggtgggcc ctgcccgtct
     1681 acaagctcct ggtcgacccc gacggccgcc cccgccgcct gcgccgcgcc ggcaggttct
     1741 agccgcggcc gtcggcgagc agccggggca ggtcgcgcat gtcgtggaag accaccgtgg
     1801 ccttgcccgc cagccggtcg ggcgagctca ggccgccggc gtagccgaag gcgcgcatgc
     1861 cggccgccct ggcccccatg aggccgtaga gggtgtcctc gaccacggcg cagcgggccg
     1921 gctcggcccc catgcgggcc gccgcgtgga ggaacaggtc gggggcgggc ttgttgttgg
     1981 cgacctcgtg gccgctgaag atgcggccct cgaaccgctc gtagaggccg gtgcggctca
     2041 gggtgaagcg gagcttgtcg tggctgccgc tggaggcgac gcaggtcgcg gccgtgatct
     2101 ggtcgagcgc ctcgacgacg ccgtcgaccg ggaccaggct ggcggcgaac gcctcccggt
     2161 agcggcgctg gaactgctcc ttccagtcgc cgggcagccg gctcccgagc tgggcctcga
     2221 tcgcctcgtc catgtactcg tccgactggc ccatgaaccg ctcgacgatc tcggcctcgg
     2281 tcagcggcca gcccagctcg gccaggtact cggtctcgac ccggatggac aggcgctcgc
     2341 tgtcgaccag cacgccgtcg cagtcgaaga tgacgagttc aaacgggccg tgcacgccgt
     2401 tcagctcccc agccggtcgg ccaggaaggc caggcccttc gggtagcggt actcgatggc
     2461 ggcgtgggtg gcgtcgaaca gctcgaagac gtggtcgacc ccgagggcgt ccagctcggc
     2521 cgagaaggcg gccgcgccaa ggtcgagcca gtactcgtcg cggcggccgg cgtccaggta
     2581 gacgccgcgc agggagcgga gctcggcggc gtggccggcg gccatgcgga ccgggtccag
     2641 ggccagccag cgggcccaca cctcgtcacg gagccggccg gtggccgggt cgaaggggag
     2701 ggtcacggtg ccgtcgggct cggccgagta gcaggcggcc atggcccaca tgttcatcag
     2761 ggcgccgtcg tcctccttgg agaacgccgg ccgtccggcg aagtcggcca agaaccgctc
     2821 gtaggagccg ccgtaggagt cgcgcagggc ccgggccgcg tgcgagatgt ccggcaggta
     2881 gcagtgctcg aacagggcat cgccggcgtg gctggccagg gcgccgaaca cgtcgggccg
     2941 gagcatcggc gtcaccatcg ccccgtagcc gccgctcgac ttgccggcga tcccgcggtg
     3001 ctcgcgggcc gggagggtcg ggtagcgccc gtccacgaag gccaccacct cgtcgcacag
     3061 gtagtccagg tagcggccgg tccccggcga gttgaggaac tggctgcccc cgagcgaggt
     3121 ccagcagtcc acgaagacca cgatcgccgg tgggctggcc ggatccgcga acagctcgtc
     3181 gaccagctcg gggacggtgg gccgcagggc ggtgcggttc caccacatgt cgacctggcc
     3241 ggtgtagccc tggatcacgt agatgctcgg gtagcgggcc ccggggtcgt cgccgatacc
     3301 cgggggcagg tagacccaca gcggccgctc ggccgggtcg ccgagcgggt tgccctccag
     3361 gacctggctc cggatggtct gctgctcgaa ccggccccgt ggctcgaccg accacggttt
     3421 cacgtggaac atccggtcac agcagcaggg cggcgtcgcg ggcgaagtag gtgacgacca
//
LOCUS       DASSWR010000214         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_7127,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000214 DASSWR010000000
VERSION     DASSWR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7127"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..599
                     /locus_tag="VFO47_03575"
     CDS             177..599
                     /locus_tag="VFO47_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET8787809.1"
                     /translation="MTQLSREAQNLNWLVSNFARSVPGVAHAIVVSADGLLMAVSERL
                     DRARADQLAAVASGLASLTQGAARCFDAGAVNQTIVEMEKGFLFVMSVSDGSCLAVLA
                     SPSCDIGLVGYEMALLVARTGDVLTPALRAELQAALPR"
     gene            627..998
                     /locus_tag="VFO47_03580"
     CDS             627..998
                     /locus_tag="VFO47_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HET8787810.1"
                     /translation="MSTEDQQENRQEPGRLVRPYAMTGGRTRPAHDDLELETLVSTTS
                     QGETSLTLGLERRSIALLCRDILSIAEISARLDLPLGVVRVLVGDMADEGLVTVHRPA
                     SVGDRPDLALLERVLYGLRTI"
     gene            979..1629
                     /locus_tag="VFO47_03585"
     CDS             979..1629
                     /locus_tag="VFO47_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HET8787811.1"
                     /translation="MDSGQYRSGSPAGESRAPTPVKIVIAGGFGVGKTTFVGAVSEII
                     PLTTEAAMTTASLGVDDTSHIQGKTTTTVAMDFGRITLDTDLILYLFGTPGQDRFWFM
                     WDDLCRGAIGAVVLVDTRRLDACFAAVDYFEQRELPFVVAINCFDGVLAHAVEDVREA
                     LVIGEDTPIVACDARVRESAKTTLIELVQHALARASAVLRSPANTRGGVGAGTSTP"
     gene            1640..2407
                     /locus_tag="VFO47_03590"
     CDS             1640..2407
                     /locus_tag="VFO47_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8787812.1"
                     /translation="MTEGGHVRANRRLWNEDADDYQRRNAPQIRDQAFTGHLAWGTWN
                     IPESELEVLGEVAGKDFLEFGCGGAQWSTALARNGARPVGFDLSERQLAHALRLREET
                     GIAVPLVQADAERVPFRDASFDVVFADYGAFLFADPYRAVPEAARVLRPGGLLAFTHI
                     SPIYDIAFAPDADHAGERLVNDYFGLYQLQDVDGTVEFNLPYGAWIRLFRDSGLVVED
                     LLEPQPGPEATSTYRDAEDLAWARRWPAEVIWRLRKP"
     gene            2559..>3397
                     /locus_tag="VFO47_03595"
     CDS             2559..>3397
                     /locus_tag="VFO47_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787813.1"
                     /translation="MGEQLVETDDLSKTYGAVRAVDRVSLSVWRGEVYGFLGPNGAGK
                     TTTLRMLAGLVRPTGGSATVHGDQPGSPRALARTGVLIEAPRFYPYLSGRDNLRVVAR
                     YAGVPDRRVEAVLDTVDLAGRGGDRYAGYSLGMKQRLGVAAALLKDPELLILDEPTNG
                     LDPAGMRDMRALLRDLGERGHTVLLSSHLLGEVQQLCDRVGVISGGRLLAETTVDDLL
                     GRAGLLVRAEPAIEARACLERLVGAGNVAQADGALRLRTEPSKAGRVSRALADAGLTL
                     TELR"
BASE COUNT          466 a         1261 c         1203 g          467 t
ORIGIN      
        1 gagcccgacc cccggcgaca gcgcccagcc ctcctcctcc gcggccaggt ccccggagga
       61 ggtcaggagc atgctgtcga gctaccgctc gggcctcgag cgcggccgca tgatggcagc
      121 cggcgaagat gccgacagag gcggcgacgc ccattcgtcc tcgaggagcg acgacgatga
      181 cgcaactcag tagggaagcg cagaacctca actggctcgt ctccaacttc gccaggagcg
      241 ttccaggggt ggcgcacgcg atcgtggtgt cggccgacgg gctcctgatg gccgtctccg
      301 agcggctgga ccgggccaga gccgaccagc tcgccgccgt ggcctcgggg ctggccagcc
      361 tgacccaggg cgccgcccgc tgcttcgacg cgggcgcggt caatcagacg atcgtggaga
      421 tggagaaggg cttcctgttc gtgatgtcgg tcagcgatgg ctcctgcctg gccgtgctag
      481 cctcgccatc ctgtgatatt ggcctggtcg gctacgagat ggcgctgttg gtggcccgta
      541 cgggggatgt gctcacgccc gccctccggg ccgagctgca agcggcgctg ccgcgatgag
      601 tacggctggg gctgggggaa ccaccgatga gcaccgagga tcagcaggag aaccggcagg
      661 agccgggtcg gctggtccga ccctatgcca tgaccggcgg gcgaacgcgt cccgcgcatg
      721 acgacctgga gctggagacg ctggtctcca ccacctcgca gggtgagacc tcgttgaccc
      781 tgggcctcga acggcggtcg atcgccctgc tgtgcaggga cattctctcc atcgcggaga
      841 tctccgcccg gctcgacctg cccctcggcg tcgtccgcgt gctcgtcggc gacatggccg
      901 atgaagggct ggtcaccgtg catcggcccg ccagcgtcgg cgaccgccca gaccttgcgc
      961 ttctcgagag ggtgctctat ggactccgga caatatagat cgggctctcc cgctggggag
     1021 tcgagggcgc cgaccccggt caagatcgtc atcgccggcg gcttcggtgt cggcaagacg
     1081 accttcgtcg gcgccgtctc cgagatcatc ccgctgacga ccgaggccgc catgaccacg
     1141 gccagcctcg gggtcgacga caccagccac atccagggca agacgaccac cacggtggcc
     1201 atggacttcg gccgcatcac cctggacacc gacctgatcc tgtacctgtt cggcacgccc
     1261 ggccaggacc gcttctggtt catgtgggac gacctgtgcc ggggcgccat cggcgccgtc
     1321 gtgctggtcg acaccaggcg gctggacgcc tgcttcgccg ccgtcgacta cttcgagcag
     1381 cgcgagctgc cgttcgtggt cgccatcaac tgcttcgacg gcgtcctggc ccacgccgtc
     1441 gaggacgtcc gcgaggccct ggtcatcggc gaggacaccc cgatcgtggc ctgcgacgcc
     1501 cgggtccggg agtcggccaa gacgaccctg atcgagctgg tccagcacgc cctggcccgc
     1561 gcctcggccg tgctgcgctc gcccgccaac acccgcggcg gcgtcggcgc cggcacctcc
     1621 accccctgac gggccgtccg tgaccgaggg cggccacgtc cgggccaacc ggaggctgtg
     1681 gaacgaggac gccgacgact accagcggcg caacgcgccc cagatccgcg accaggcctt
     1741 caccggccac ctcgcctggg gcacctggaa catccctgag tcggagctgg aggtgctcgg
     1801 cgaggtcgcc ggcaaggact tcctggagtt cggctgcggc ggcgcccagt ggtcgacggc
     1861 cctggcccgc aacggcgccc ggccggtcgg cttcgacctg tccgagcgcc agctcgccca
     1921 cgccctgcgc ctgcgggagg agacggggat cgccgtcccg ctggtccagg cggacgccga
     1981 gcgggtcccg ttccgcgacg ccagcttcga cgtggtgttc gccgactacg gggcgttcct
     2041 gttcgccgac ccctaccggg cggtgcccga ggcggcccgg gtcctgcgcc ccggcggcct
     2101 gctcgccttc acccacatca gccccatcta cgacatcgcc ttcgcccccg acgccgacca
     2161 cgccggtgag cggctggtca acgactactt cgggctctac cagctgcagg acgtggacgg
     2221 cacggtcgag ttcaacctgc cctacggggc ctggatccgg ctgttccgcg acagcggcct
     2281 ggtggtcgag gacctgctcg agccccagcc cgggccggag gcgaccagca cctaccgcga
     2341 cgccgaggac ctggcctggg cgcgccgctg gccggccgag gtcatctggc ggctccgcaa
     2401 gccctgatcc catcccgcta ccaccggcgc ggtagccggg cccgccgccg ctgctccgcc
     2461 gggagcagcc ggatcgcctt cccccgggcg acgacgccgc cggccccggc cgcgaccatc
     2521 gtctctgagc acacgaccgg tacgacgagg gcagggacat gggcgagcag ctggtggaga
     2581 ccgacgacct gagcaagacc tacggcgccg tcagggccgt cgaccgggtc agcctgtcgg
     2641 tgtggcgcgg ggaggtgtac gggttcctcg gccccaacgg ggccggcaag acgaccaccc
     2701 tgcggatgct ggccgggctg gtcaggccga ccggcgggtc ggcgacggtc catggcgacc
     2761 agccggggag cccgcgggcc ctggcccgga ccggcgtcct gatcgaggcg ccccgcttct
     2821 acccgtacct gtcggggcgc gacaacctgc gcgtggtcgc ccgctacgcc ggggtccccg
     2881 accggcgggt cgaggcggtc ctggacacgg tcgacctggc cggccggggc ggcgaccggt
     2941 acgccggcta ttccctcggc atgaagcagc gcctcggggt ggcggcggcc ctgctgaagg
     3001 acccggagct gctgatcctc gacgagccga ccaacgggct cgacccggcc ggcatgcgcg
     3061 acatgcgggc cctgctccgc gacctcggcg agcgcggcca caccgtcctg ctgtcctcgc
     3121 acctgctggg cgaggtgcag cagctctgcg accgggtcgg ggtcatctcc ggcggccggc
     3181 tgctggccga gaccaccgtc gacgacctgc tcggccgggc cggcctgctg gtgcgggccg
     3241 agccggccat cgaggcccgg gcctgcctgg agcggctggt cggcgccggc aacgtcgccc
     3301 aggccgacgg cgccctccgg ctgcggaccg agccgtcgaa ggcaggcagg gtgagccgcg
     3361 ccctggccga cgccggcctg accctgaccg agctgcg
//
LOCUS       DASSWR010000215         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_969408,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000215 DASSWR010000000
VERSION     DASSWR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969408"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFO47_03600"
     CDS             <1..521
                     /locus_tag="VFO47_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787814.1"
                     /translation="IATISLAALLQLAPTQRPFVLAVYLLIMGGLATHLLMTWLRLTF
                     PRPPKSPFDAALAARPAPPRPPAELDRLARLLALSSASALHAHTRLRNELRPVAADRL
                     RWSLAVDLDRDPAAARAALGEAGWALLARDQRDLPSREAPGLPPAALADLIANLERIG
                     RRGPGNPDGVPR"
     gene            533..1528
                     /locus_tag="VFO47_03605"
     CDS             533..1528
                     /locus_tag="VFO47_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8787815.1"
                     /translation="MADGGLGELRRLTQRVLDEVERAVVGKRGALELVLLGLLADGHV
                     LLDDYPGLAKTLIARSFARVTSLSFARVQFTPDLMPSDVTGSSVYNQRLGDFEFRPGP
                     VFTNLLLGDEINRSPPKTQAALLEAMQERQVTIDGVTRSLERPFLVVATQNPIEFEGT
                     YPLPEAQLDRFLMRLSVGYPSAEAEWRMLERRLERTADEVELAQVATPADVLAMQRAV
                     EEVHVAESVGRYIVELVGATRASPRVQVGSSPRGSLALLKVARAKAALAGRDFVTPED
                     VKAVTIPTLAHRLILRPELWVRRVRTEDVVAELLEQVPTPPPEAEPAPVRAQPGR"
     gene            1525..2847
                     /locus_tag="VFO47_03610"
     CDS             1525..2847
                     /locus_tag="VFO47_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8787816.1"
                     /translation="MNRRPSDKIAVYVGVAALVLLAGLALGRPELVAVAAPLAVLVVA
                     GLAGARDPELAAEVTVDRERALVGDELTVELRLTAATRIERLEVLLVVPPGMEGPEGG
                     PGGSSPAPRTAGTARTWATALTLGAGEERAIAVRLTCRRWGAYWLGDVHLRVHDRFRL
                     FTWERHLDRHAPLKVFPVPDALRALVRPLETQVSTGSHVAAQRGDGIEFADLRPFLPG
                     DRPRSINWRATARRGGLMVNQRHPERATDVVLFLDSFLDVRGPAGSTLDQAVGAAASL
                     AAAYLRQRDRVGLVTFGGFVQWLQPGGGQTALYRLLDTLMETQVFATAAWKGIRHLPP
                     RTLPAKALIVALTPLVDERGVAALFDLLARGYDLAVVDISPLTHAGVAAGEWGELARR
                     LWALERETLRHRYQRLGAAVVEWTDAMHLQQVLLEVEACRRRAGLAPG"
     gene            2814..>3071
                     /locus_tag="VFO47_03615"
     CDS             2814..>3071
                     /locus_tag="VFO47_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787817.1"
                     /translation="MPAPRRARTRLACGLAATAVAAALAAAPLAAVSGPLRGALAWLA
                     GLGLVLAAAAALRTGALLGPALVVFLAEYTVVLTGRGGRVDA"
BASE COUNT          333 a         1237 c         1113 g          388 t
ORIGIN      
        1 tcatcgccac catcagcctg gccgcgctgc tccagctcgc ccccacccag cggcccttcg
       61 tgctggccgt ctacctgctg atcatgggcg ggctggccac ccacctgctg atgacctggc
      121 tgcggctgac cttcccgcgg ccgccgaagt cgccgttcga cgccgccctg gccgcccggc
      181 cggccccgcc caggccgccg gccgagctgg accggctggc ccggctgctc gccctgtcca
      241 gcgccagcgc cctgcacgcc cacacccggc tccggaacga gctgcggccc gtggccgccg
      301 accggctgcg ctggtcgctg gccgtcgacc tggaccgcga cccggccgcc gccagggccg
      361 ccctcggcga ggccggctgg gccctgctcg cccgcgacca gcgcgacctg ccgtcccggg
      421 aggctcccgg cctccctccg gccgcccttg ccgacctgat cgccaacctg gaacggatcg
      481 gccggagggg tccggggaac cccgacgggg tgccccggtg aagcaaggcg caatggctga
      541 cgggggcctg ggggagctgc gcaggctcac ccagcgggtc ctagacgagg tcgagcgggc
      601 cgtggtcggc aagcgcggcg ccctcgagct ggtcctgctc gggctgctgg ccgacgggca
      661 cgtgctcctg gacgactacc ccggcctggc caagaccctg atcgcccggt cgttcgcccg
      721 ggtgacgtcg ctgagcttcg cccgggtcca gttcaccccc gacctgatgc cgtcggacgt
      781 gaccggctcg tcggtctaca accagcgcct gggcgacttc gagttccgcc ccggcccggt
      841 gttcaccaac ctgctcctcg gcgacgagat caaccgctcc ccgcccaaga cccaggccgc
      901 cctgctggag gcgatgcagg agcggcaggt gacgatcgac ggcgtgacca ggtccctgga
      961 gcggccgttc ctggtcgtgg ccacccagaa cccgatcgag ttcgagggca cctacccgct
     1021 gcccgaggcc cagctcgacc ggttcctgat gcggctgtcg gtcggctacc cctcggccga
     1081 ggccgagtgg cggatgctgg agcggcgcct ggaacggacc gccgacgagg tcgagctggc
     1141 ccaggtggcg acgccggccg acgtgctcgc catgcagcgg gcggtcgagg aggtccacgt
     1201 ggccgaaagc gtcggccgct acatcgtcga gctggtgggg gcgacccggg ccagcccgcg
     1261 ggtccaggtc ggctccagcc cgcggggcag cctggccctg ctgaaggtgg ccagggccaa
     1321 ggccgccctg gccggccgcg acttcgtcac ccccgaggac gtcaaggcgg tcaccatccc
     1381 caccctggcc caccggctca tcctgcggcc ggagctgtgg gtgcgccggg tccgcaccga
     1441 ggacgtcgtg gccgagctgc tggagcaggt cccgaccccg ccccccgagg ccgagccggc
     1501 cccggtccgc gcccagcccg gccggtgaat cgccggccga gcgacaagat cgccgtctac
     1561 gtcggggtgg cggcgctggt cctgctggcc gggctggccc tgggccggcc cgagctggtg
     1621 gcggtcgcgg cccccctggc cgtgctggtg gtggccggcc tggccggcgc ccgcgacccc
     1681 gagctggccg ccgaggtcac cgtcgaccgg gagcgggccc tggtcggcga cgagctgacc
     1741 gtcgagctgc gcctgaccgc cgccacccgg atcgaacgcc tggaggtgct cctggtcgtc
     1801 cccccgggca tggagggccc cgaggggggg ccggggggct caagcccagc cccccggacg
     1861 gcaggaacgg cccggacctg ggccacggcc ctcaccctgg gcgcggggga ggagcgggcc
     1921 atcgccgtcc ggctcacctg ccggcgctgg ggcgcctact ggctcggcga cgtccacctg
     1981 cgggtccacg accgcttccg gctgttcacc tgggaacgcc acctggaccg gcacgccccc
     2041 ctcaaggtgt tccccgtccc cgacgccctc cgggccctgg tccggccgct ggagacccag
     2101 gtctcgaccg gcagccacgt cgccgcccag cgcggcgacg gcatcgagtt cgccgacctg
     2161 cggccgttcc tgcccggcga ccggccccgc tcgatcaact ggcgggccac cgcccgccgc
     2221 ggcggcctca tggtcaacca gcgccacccc gagcgggcca ccgacgtggt cctgttcctg
     2281 gacagcttcc tcgacgtccg cgggcccgcc ggcagcaccc tcgaccaggc cgtgggcgcg
     2341 gccgcctcgc tggccgcggc ctacctgcgc cagcgggacc gggtcggcct ggtcaccttc
     2401 ggcgggttcg tgcagtggct ccagcccggc ggcggccaga ccgccctgta ccggctgctc
     2461 gacaccctga tggagaccca ggtgttcgcc accgcggcct ggaaggggat ccgccacctg
     2521 ccgccccgga ccctgccggc caaggccctg atcgtcgccc tgaccccgct ggtcgacgag
     2581 cgcggggtcg cggccctgtt cgacctgctc gcccgcggct acgacctggc cgtggtcgac
     2641 atctccccgc tgacccacgc cggcgtggcc gccggcgagt ggggcgagct ggcccggcgc
     2701 ctgtgggccc tggagcggga gacgctgcgc caccgctacc agcgcctggg cgcggccgtg
     2761 gtcgagtgga ccgacgccat gcacctgcag caggtgctcc tggaggtgga ggcatgccgg
     2821 cgccgcgccg ggctcgcacc cggctagcct gcggcctggc cgccaccgcg gtcgccgccg
     2881 ccctcgccgc cgcccccctg gccgcggtca gcgggccgct gcgcggggcc ctggcctggc
     2941 tggccggcct cggcctggtc ctggcggccg cggccgccct gcggaccggg gcgctgctcg
     3001 gcccggccct ggtcgtgttc ctggccgagt acacggtggt cctgaccggc cggggcggcc
     3061 gggtcgacgc c
//
LOCUS       DASSWR010000216         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_573913,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000216 DASSWR010000000
VERSION     DASSWR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573913"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VFO47_03620"
     CDS             complement(<1..172)
                     /locus_tag="VFO47_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET8787818.1"
                     /translation="MVTVGLLVRLVAKPGKEAEVTAFLEGGLALVEEEPATIAWFAVR
                     LGPSEFGIFDAFP"
     gene            243..539
                     /locus_tag="VFO47_03625"
     CDS             243..539
                     /locus_tag="VFO47_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787819.1"
                     /translation="MAHSLCIAWRGRFAHRGVLVDHAHQASPKEKPAVGDPQRLSDNE
                     QLEILALLEAVSTECSAMRAARALAELMGHLHSLGQRIAGELPDQNRDIKPARR"
     gene            602..736
                     /locus_tag="VFO47_03630"
     CDS             602..736
                     /locus_tag="VFO47_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787820.1"
                     /translation="MVGIGCPFGQARSSVTCQRRFEAEEGHMAKYLILIYGPESATPR
                     "
     gene            1001..1891
                     /locus_tag="VFO47_03635"
     CDS             1001..1891
                     /locus_tag="VFO47_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787821.1"
                     /translation="MSQPAIQAVALAKRFGSVQALRDLDLEVQPGEVFGYLGPNGAGK
                     TTTIRLLLDFIRPTSGRIEVLGGSGADPAVRRRIGYLPAELPVDRRWTAQDLIDFYGK
                     LRGGVDAGWVRELLGRFDLDPTRRVGELSTGNRRKIGIIQAVIHRPELLVLDEPSSGL
                     DPLLQYEFQLLVRELAGAGATVFLSSHVLPEVEALAGRVAILRRGELVTVAGIQELRQ
                     QARQRIELRLARPADATWFASVPGVVEAQADDTRIRLVVEGPVGPALQAAAGLEVQRI
                     VTHEADLEDVFLSYYQEQPA"
     gene            1888..2688
                     /locus_tag="VFO47_03640"
     CDS             1888..2688
                     /locus_tag="VFO47_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8787822.1"
                     /translation="MIVAARLVRDRRWATLGWALGFVGLTLFTVALYPSIKDQPSFDE
                     LLADLPDTFRSVIGYDAAVPLRSPAGYLQARLFSTLAPLLAVVFGIGAGARAIGGSEE
                     AGTLELLLANPVTRGRVLVERYLATFGLLAGLVAVFGLALLVLGPPFEALEGVPTGNV
                     VAACAAVFALGLLHGSLAFTIGAVAGRRGIAVALATAVAVAGYLVETVLAAAGDLGAV
                     RQLSPWHWYLDRNLLAEGAAPAAVMLPLAIAALLVGVAWVVFRRRDLR"
     gene            2830..4041
                     /locus_tag="VFO47_03645"
     CDS             2830..4041
                     /locus_tag="VFO47_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787823.1"
                     /translation="MQARTRVLLAWSLWLATFGCCAAGLLVTLAVTRPLTLAVLAQGA
                     AYALAYPLGYATIGLVLSLRRPANPIGWLYAAAGLTWSLLIPLEPWVAQLVRDQRPLP
                     LAAQVAYVVLQPTWAPAIGCGITLPLLLLPDGRLRSRRWRPVAVAAVAGPVVVMVAGS
                     LAPEPPEEALFPNPLALGGAAGRAAAMAAWVGVALFLVGLVAAVACLVLRFRAARGVQ
                     RQQLRWVAAGATATVAAIWVLVPVALSGVAPAVPVVLFPLASLCVPVAVAVAMLRYRL
                     WDLDRLVSRTVTYTVVTAVLALPYLLILPAVTRLAGEAGSLAVAAVTLAAVAGFAPLR
                     RRVRDRVDRRFNRHRYDAARTVAGFAARLRDQVDLEALQGELLTAVDETVQPTRAWLW
                     LRPPATPRLPR"
     gene            complement(4085..4528)
                     /locus_tag="VFO47_03650"
     CDS             complement(4085..4528)
                     /locus_tag="VFO47_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787824.1"
                     /translation="MLVVVRRMLQCLPRPWWPTQHGGPFGKLPSMRRRRVWARRLDQL
                     LDAASRNPSAWRFPHPDFEAPLQLSTRVVWPCRPELLAIMAVLQEYRQPISSAALQQL
                     KAFLTDPGVSALFGTDPGAARQAAQRLQHSFTGHPEPRDPPRTTR"
     gene            complement(4711..6003)
                     /locus_tag="VFO47_03655"
     CDS             complement(4711..6003)
                     /locus_tag="VFO47_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014053731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET8787825.1"
                     /translation="MSATLAVVVFLGAYGLIATERVNRVAVALGGAGLMLALGLVSSA
                     DAFHSERFGIDWDVIFLLLGMMVIVGVLKQTGLFDYLAIWSAKRARGRPFRILAMLVT
                     ITALASALLDNVTTVLLVAPVTFLICDRLAISAVPFLIAEALASNIGGTATLIGDPPN
                     IIIASQADLSYTDFLVNLAPLVVVLMVLFVGLSRWLFRDALVYRPDRARELLTLDERE
                     ALNDRPLLVRSLAVLALVTVAFALHGVLHYEPSVVALLGAGLLLLIARRDVQPFLREV
                     EWPTLAFFMGLFIMVGALIKTGVIAQLAEAVAAATGGSLLVASMLLLWVSGALSAIVD
                     NIPYVATMAPVTATLVQDLGGGRDATVLWWSLALGADLGGNATAIGASANVVILGLAQ
                     RAGQPISFLEFTKYGAVVAVATMLACVPYLLLRYFVLA"
     gene            complement(6000..6449)
                     /locus_tag="VFO47_03660"
     CDS             complement(6000..6449)
                     /locus_tag="VFO47_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8787826.1"
                     /translation="MRARDLATPYPSLAPDTPAEEAARLLAEEAVEGVFVQDDQGELQ
                     GLVSDTSLLAFLLPRYLAEDRALVRVLGEDVADALWQRLRGRPVRDLLPASTAGLPEV
                     DADDTLVSVAATLARTSASLVAVRGRDGRLLGGITTSQLLTRLLGTR"
BASE COUNT          958 a         2294 c         2293 g          976 t
ORIGIN      
        1 cgggaaaggc gtcgaagatc ccgaattccg agggtccaag gcggaccgcg aaccaggcga
       61 tggtggccgg ttcctcctcc accagggcca agccgccctc aaggaaggcg gtcacctcgg
      121 cctccttgcc gggtttggcc accagacgaa caagcagccc cacagttacc atcgccgcca
      181 tcctttcgac ggagccaccg ccatcacaag cacaaaccac aagtccgggc atcctactgc
      241 gggtggctca ctcgctctgc atcgcctggc gcggaaggtt tgcccatcgg ggcgtgctgg
      301 tcgatcatgc ccaccaagca agccccaagg agaagcctgc ggtgggcgac cctcaacgac
      361 taagcgacaa cgagcagctt gagattctcg ccttgctcga agccgtcagc accgagtgct
      421 cggccatgcg cgccgcccgc gccctggccg agctcatggg tcatctccat agtctggggc
      481 aacgcatcgc cggtgagctg cccgaccaga accgcgacat caagccagcc aggcgttaac
      541 gcattcgccc gcctcacttg tcaggacctc ctacggagtc acggccggtc ctgacgttgg
      601 cgtggtggga atcggctgtc catttggcca ggctcgttcg tcggtcacgt gtcagcgccg
      661 cttcgaggcc gaggagggcc acatggccaa gtacctgatc ctcatctacg ggccggagtc
      721 cgccacgcca cggtgaggcg ccgtggagag agggccttcg ccagaatcgt gacggctgtg
      781 gctgggctga cctggtgtgg tgtacgggtt agcagattgg ttggccgaag tcggggtcga
      841 acgggaggcg gtcggagggg cagacggtcc acgcgccggg acgctgagtt cgaccacaaa
      901 gccgccggtg gcctgtatga cgcaggtgct acgctaggcg tggcgcgccg ggaagcctgg
      961 tcggcagttg acccagcgtc gacccgtcgg ggagcacacg gtgagccagc ccgccatcca
     1021 ggccgttgct ctggccaagc gcttcgggtc ggtgcaggcc ctgcgcgacc tcgatctcga
     1081 ggtccagccg ggcgaggtat tcggctacct gggacccaac ggagccggca agaccaccac
     1141 catccggcta ctgctcgact tcatccgacc caccagcggc cgcatcgaag tcctcggggg
     1201 cagcggcgcc gacccggcgg tgcggcgccg gattggctac ctgcctgccg agctgccagt
     1261 cgaccgccgc tggaccgccc aagacctgat cgacttctac ggcaagctgc gcggcggcgt
     1321 ggatgccggc tgggtgaggg agctgctggg ccgcttcgac ctcgacccga cccggcgggt
     1381 cggggagctg agcaccggca accggcgcaa gatcggcatc atccaggcgg tcatccaccg
     1441 tcccgagctg ctcgtgctgg acgagccgtc cagcggcctg gatccgctgc tgcagtacga
     1501 gttccagctg ctggtccggg agctggccgg ggcgggagcg accgtgttct tgtccagcca
     1561 tgtgctgccg gaggtcgagg ccctggccgg ccgggtggcg atcctgcgcc ggggtgagct
     1621 ggtcaccgtg gccggcatcc aggagttgcg ccagcaggcc cgccagcgaa tagagctgcg
     1681 gctggcccgg ccggccgacg ccacatggtt cgccagcgtt cccggcgtgg tcgaggccca
     1741 ggccgacgac acccgcatcc gcctggtcgt cgagggtccg gtgggtccgg ccctgcaggc
     1801 cgcggccggg ctggaggtgc agcgcatcgt cacccacgag gccgacctgg aggacgtctt
     1861 cctcagctac taccaggagc agccagcatg atcgtcgccg cccgcctggt ccgcgaccgc
     1921 cgctgggcga cgctggggtg ggcgctcggc ttcgtcggcc tcaccttgtt caccgtcgcc
     1981 ctgtacccct ccatcaagga ccagccgtca tttgacgagc tgctcgccga cctccccgac
     2041 accttccggt cggtgatcgg ctacgacgca gccgtcccgc tgcggtcgcc ggccggctac
     2101 ctccaggccc ggctgttctc caccctcgcc ccgctgctgg cggtggtgtt cggcatcggc
     2161 gccggagccc gggcgatcgg cggcagcgag gaggccggca ccctggagct gctgctggcc
     2221 aaccccgtca cccgcggccg cgtcctggtt gagcgctacc tggccacctt cggactgctg
     2281 gccggcctgg tcgccgtgtt cggcctggcc ctgctggtcc tcggcccccc gttcgaggcc
     2341 ctggagggcg tcccgaccgg caacgtggtc gcggcctgcg ccgccgtctt cgccctcggc
     2401 ctgctgcacg gctcgctcgc cttcaccatt ggcgctgttg ccggccgccg cggcatcgcc
     2461 gtcgcgctgg ccaccgccgt ggcggtcgcc ggctacctgg tcgagaccgt actggccgcc
     2521 gccggggacc tgggcgccgt gcgccagctc agcccctggc attggtatct cgaccggaac
     2581 ctgctcgccg agggcgccgc ccccgccgcc gtcatgctcc cactggccat cgctgccctc
     2641 ctggtcggcg tcgcctgggt cgtgttccgc cgacgcgacc tgcgatgaac ctacaggaac
     2701 tcaggcgttg gcttgacggt accgcttggg ctgccattgg aggaaccgta acgttctcgt
     2761 accggcgtcc tgccaatttc ggctactagg tagccctgag cgcagtggcc cgagagcagg
     2821 gtggtgagga tgcaggcgag gaccagggtc ctgcttgcct ggtcgttgtg gctggccacc
     2881 ttcggctgct gcgccgccgg cctgctggtc actctggcgg tgacccggcc actcaccctc
     2941 gccgtgctgg cacaaggcgc cgcctacgcg ctggcctatc cgctcggcta cgccaccatc
     3001 gggctggtgc tgagcctacg gcggccagcc aacccgatcg gctggctgta cgccgccgcc
     3061 ggattgacct ggtcactgct catccccctg gagccctggg tcgcccagct ggtccgcgac
     3121 cagcgaccgc tgccgctggc cgcccaggtc gcctacgtcg tgctgcagcc cacgtgggca
     3181 ccggcgatcg gctgcggcat caccttgccg ctgctgctgc tgcccgacgg gcggctacgc
     3241 tcgcgccgct ggcggccggt ggcggtggcc gccgtggccg ggccggtggt tgtgatggtg
     3301 gccggcagcc tggccccgga gcccccggag gaggcgctgt tccccaaccc gctggcgctt
     3361 ggcggcgcgg ccggcagggc cgcggcgatg gccgcctggg tgggcgtggc cctgttcctg
     3421 gtcggcctgg tggccgcggt cgcctgcctg gtgctgcggt tccgtgccgc ccgcggagtc
     3481 cagcgtcagc agctgcgctg ggtcgccgct ggcgccaccg ccacggtcgc cgccatctgg
     3541 gtattggtcc cggtggccct gtctggggtt gcccccgccg tcccggtggt gctcttcccg
     3601 ctggcgtcgc tgtgcgtgcc ggtcgcggtc gcggtggcga tgctgcgcta ccgcctctgg
     3661 gacctggacc gcctggtcag ccgcaccgtc acctacaccg ttgtcaccgc cgtgctcgct
     3721 cttccctacc tgctcatcct cccagccgtg acccgcctgg ccggcgaggc cggcagcctg
     3781 gcggtggccg ccgtgaccct ggccgcggtg gccggattcg cgccgctgcg ccggcgggtc
     3841 cgggaccggg tggatcggcg cttcaaccgt caccgctatg acgccgcccg caccgtggcc
     3901 gggttcgccg cccgcctgcg cgaccaggtc gacctggagg ctctccaagg cgagctgctg
     3961 accgcggtcg acgagaccgt gcagccgacc cgggcgtggc tgtggctgcg cccaccggcg
     4021 accccgcggc tgccgcggta gcgcagctac cgacacgtca gatgcttgcg ctgcaccgga
     4081 gcgtctagcg tgtggttcgc ggtggatctc gaggttctgg gtgaccggtg aagctgtgct
     4141 ggagtcgctg ggcggcctga cgcgccgcac cggggtcggt gccgaacaga gctgaaacgc
     4201 ctggatcggt caggaaggcc ttcagctgtt gtagagccgc cgacgagatg ggctggcgat
     4261 actcctggag cacggccatg atggccagca gctcgggacg gcatggccag acgactctgg
     4321 tcgacagctg aagcggagcc tcgaaatcgg gatgagggaa ccgccaagcg gaagggttgc
     4381 ggctggccgc gtcgaggagc tgatccagcc ttcgagccca gacccgacga cgccgcatac
     4441 tgggcagctt gccaaacggg ccaccatgct gggtcggcca ccaagggcgg ggcaggcatt
     4501 gcagcatccg acgcacgacg accagcacgg gacgctcctg tccgacggag aacacgccgc
     4561 cgaccaggct tcacggcaca ccagtcaagg actgtatctg catcgtcaag ccttgttcac
     4621 aggaccgccg acacggggct gcgtggctgt tcggtcccgt cggttctccc tcgatcagtc
     4681 cgtcgtgagc cattgcatgc agcggacggg tcaggcgagg acgaagtagc gaagcagcag
     4741 atagggcaca caggccagca tggtggcgac cgcgaccacg gcgccgtact tggtgaactc
     4801 caggaagctg atgggttggc cggcgcgttg ggccaggccg aggataacca cgttggcgct
     4861 ggcgccgatg gcggtggcgt tgccgcccag gtccgcgccg agcgccagtg accaccacag
     4921 cacggtcgca tcccgcccgc cccccaggtc ctggacgagc gtagcggtca ccggggccat
     4981 ggtggccaca taggggatgt tgtcgacgat ggccgacagc gccccagaga cccacagcag
     5041 cagcatgctg gccaccagca ggcttccacc ggtggccgct gccaccgcct cggcgagctg
     5101 ggcgatcacg ccggtcttga tgagggcgcc gaccatgatg aacaggccca tgaagaaggc
     5161 cagagtgggc cactcgacct ctcgcaggaa gggctggacg tcgcggcggg cgatcagcag
     5221 caagagcccg gccccgagca gcgcaaccac cgacggctcg tagtgcagca ctccgtgcag
     5281 cgcgaacgcg acggtgacca gggcgagtac ggccagagag cggaccagca ggggccggtc
     5341 gttgagggcc tcgcgctcgt ccagggtcag cagctcgcgg gcgcggtcgg gccggtagac
     5401 cagggcgtcg cggaacagcc agcggctcag cccgacgaac agcaccatga ggaccaccac
     5461 cagcggggcc aggttgacca ggaagtcggt gtaggacaga tccgcctggc tggcgatgat
     5521 gatgttgggc gggtcgccga tcagggtggc ggtgccaccg atattggacg ccagcgcctc
     5581 ggcgatcagg aacggtaccg ctgagatggc cagccggtcg cagatcagga aggtgaccgg
     5641 ggccaccagc agcacggtgg tgacgttgtc cagcagggcc gaggccaagg cggtgatggt
     5701 gaccagcatt gccaggatcc ggaatggccg gccgcgggcc cgtttcgccg accagatggc
     5761 cagatagtcg aacaggccgg tctgcttgag caccccgacg atcaccatca tgcccagcag
     5821 caggaagata acgtcccagt cgatcccaaa gcgctcggag tggaaggcgt cggccgagga
     5881 caccagcccc agggccagca tgagcccggc gccgccgagg gcgaccgcca cccgattgac
     5941 cctctcggtg gcgatcaggc cgtaggcacc caagaagacg accacggcca gggtggcgct
     6001 catcgggtgc ccagcagccg ggtgaggagt tgggaggtgg tgatgccgcc cagcagccgg
     6061 ccgtcgcggc cgcgcaccgc gaccagggat gcgctggtcc gggccagggt ggcggccaca
     6121 ctgaccaagg tgtcgtcggc gtcgacctcg ggcagcccgg cggtggaggc tgggagcagg
     6181 tcccgcaccg gtcggccgcg cagccgctgc cacagggcgt cggcgacgtc ctcgccgagg
     6241 acgcggacca gcgcccggtc ttctgcgaga tagcgcggca gcagaaaggc cagcagggac
     6301 gtgtccgaca cgagcccttg gagctcgcct tggtcgtctt ggacgaagac gccctcgaca
     6361 gcttcctcgg ccagcagccg agcggcctcc tcggctgggg tgtcgggcgc cagcgaggga
     6421 tatggcgtgg ccagatctcg cgcacgcatc aggtcggccc ctccccccct ggccaaccgc
     6481 cagccgcccc ctcaccggta cccggtcgcc gaccaccgcc a
//
LOCUS       DASSWR010000217         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_461042,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000217 DASSWR010000000
VERSION     DASSWR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461042"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFO47_03665"
     CDS             <1..946
                     /locus_tag="VFO47_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8787827.1"
                     /translation="SRETRARVKRVAEELGYRHHMMARGLRRGFSSTVGVVVADLGNP
                     YTAPVMRGLQHALERADYIALMAESRDDSQALDRAVGHLLGRQVDGLVLLAARWGDLD
                     RILGWMSRVPVVLAVRGLSGSGVPEVTHDDLGGGALAAGHLAELGHRRVVQLPGPQDV
                     QPFKDRHDSFAEAATGLGLEAWAAPAASRPVFDEGRRLMALALADGRWATGVFAHNDA
                     MAMGAVRALREAGLDCPRDVSVVGYNDVPMADCFDPPLTTIRLDGAEVGRQSAESVLA
                     AIRGLPIPTRATPVPAELVVRASARRPVARRARRRAAP"
     gene            complement(900..1310)
                     /locus_tag="VFO47_03670"
     CDS             complement(900..1310)
                     /locus_tag="VFO47_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787828.1"
                     /translation="MSELSRLLVKSVRSLPAEEQDKLLEAMLEPRLAAWSGPGGGPDL
                     PPSPVHRAYSPELGYELTLPPLGPETGGPWQSVPVRLSTDQHERLKQWCQANGFTMAV
                     VLRGLVARFLDDQAGRGRRATAPPAGEPGEPPAS"
     gene            complement(1353..2621)
                     /locus_tag="VFO47_03675"
     CDS             complement(1353..2621)
                     /locus_tag="VFO47_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787829.1"
                     /translation="MRSSYLAILRLPHVRPLLLASLVGRLPTATGPLSVVLFVQEVTG
                     SLARAGAASAAIALASGLLAPVRGRLVDRLGQRRCLPPMALVFAAALAGMVAVAGPSP
                     AEVAAMIALAAAAGAAAPPLGASMRVLWLSLVGQGPRLQTAYALDAVLDELLFVIGPL
                     LAGALATLYQPAAGVLATAGLAVAGTLGFVASPVSKAQAGSRAAAATGRGGWAGALRG
                     PGMRTLTLSLAGVGAAIGIWEIGLVGAAREAGSPDAASLLLAAWAAASGLGGLWYGAR
                     TWRRSPGHRYLALLALLVLAGAPMAAAATPLALGAVVALVGLVLAPLESSAYVLAAEL
                     APPGTLTESGTWLTTAINVTGAAGLTVAGVLVDQAGVPATLAAACACTAAGLLVALAG
                     RDRLGAAPYRGRHEVGHAATRQLRRERRML"
     gene            2930..>3278
                     /locus_tag="VFO47_03680"
     CDS             2930..>3278
                     /locus_tag="VFO47_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787830.1"
                     /translation="MSARLRWYVGSLTVAAGFVLAFLLTRLDLGRAREVAGAIAVFAL
                     FMIAGELVLLRINAQNRLKEVSITSTFAYGLVPLAGTAVAVLVFLLGSVVSDLGRRKG
                     AIKTLFNAAQYVLA"
BASE COUNT          431 a         1190 c         1276 g          381 t
ORIGIN      
        1 gtcgcgtgag acgcgggccc gggtcaagcg ggtggccgag gagctcggct accggcatca
       61 catgatggcc agggggctgc gccgcgggtt cagctcgacg gtcggcgtgg tcgttgccga
      121 cctcgggaac ccctacaccg ccccggtcat gcgcgggctc cagcacgcgc tggagcgggc
      181 cgactacatt gcgctgatgg ccgagtcgcg cgacgactcc caggcgctcg accgggccgt
      241 cggccacctc ctcggccgcc aggtggacgg gctggtgctg ctggccgcgc gctggggcga
      301 cctggaccgg atcctcggct ggatgtccag ggtcccggtg gtcctggccg tgcgcggcct
      361 gtccggctcc ggggtgccgg aggtgaccca cgacgacctc ggcgggggcg ccctggcggc
      421 cgggcacctg gccgagctcg gccaccgccg ggtcgtccag ctgccgggac cgcaggacgt
      481 gcagccgttc aaggaccgtc acgacagctt cgccgaggcc gcgaccggcc tcgggctgga
      541 ggcctgggcg gcgccggcgg ccagccggcc ggtcttcgac gaggggcggc ggctgatggc
      601 cctggccctg gccgacgggc gctgggccac cggggtgttc gcccacaacg acgccatggc
      661 catgggggcg gtccgggcgc tgcgcgaggc cggcctcgac tgtccccgcg acgtctcggt
      721 ggtcggctac aacgacgtgc ccatggccga ctgcttcgac ccgccgctca ccaccatccg
      781 gctcgacggc gccgaggtcg gccgccagtc cgcggagtcc gtgctggcgg ccatccgggg
      841 gctgcccatc ccgaccaggg cgacccccgt cccggccgag ctggtggtgc gggccagcgc
      901 taggaggccg gtggctcgcc gggctcgccg gcgggcggcg ccgtagcccg gcggccgcgg
      961 ccggcctggt cgtccaggaa gcgcgcgacc aggccgcgca ggaccacggc catggtgaac
     1021 ccgttggcct ggcaccactg cttgagccgc tcgtgctggt cggtggagag gcggacgggc
     1081 acgctctgcc agggcccgcc ggtctcgggg ccgagcggtg ggagggtgag ctcgtagccg
     1141 agctccggac tgtaggcccg gtgcaccggg gacggcggca ggtcggggcc accacccggc
     1201 cccgaccagg cggccagccg gggctcgagc atggcctcca ggagcttgtc ctgctcctcg
     1261 gccgggaggg aacggacgga cttgaccagc aggcgcgaca gctctgacat cggtgcaccc
     1321 ctcattgata cccgaactga tatcgggcga tatcagagca tgcgccgttc ccgccgcagt
     1381 tgtcgagtcg cggcgtgccc cacctcgtgg cggccccggt agggggccgc gcccaggcgg
     1441 tcgcggccgg ccagggcgac cagcaggccg gcggccgtgc aggcgcaggc ggcggccagg
     1501 gtggccggca ccccggcctg gtcgaccagg accccggcga cggtcaggcc ggccgccccg
     1561 gtgacgttga tggcggtggt cagccaggtg cccgactcgg tcagggtgcc cggcggagcc
     1621 agctcggcgg ccaggacgta ggccgagctc tccagggggg cgaggacgag cccgaccagg
     1681 gcgaccaccg cccccagggc caggggcgtg gccgcggccg ccatcggggc gccggccagg
     1741 acgagcaggg cgagcagggc gaggtagcgg tggccgggcg accggcgcca ggtcctggcc
     1801 ccgtaccaga ggccgccgag accgctggcc gcggcccagg cggcgagcaa cagggaggcc
     1861 gcatcgggcg agccggcctc cctggccgcc ccgaccaggc cgatctccca gatgccgatt
     1921 gcggcgccga cgccggcgag cgacagggtc agggtgcgca tgcccggccc gcgcagggct
     1981 ccggcccagc cgccccggcc ggtcgcggcc gcggcccggc tgcccgcctg cgccttcgac
     2041 accggcgagg ccacgaaccc gagcgtcccg gccacggcca gcccggccgt ggccagcacc
     2101 ccggccgccg gctggtacag ggtggcgagc gcaccggcca gcagcggccc gatcacgaac
     2161 agcagctcgt cgaggacggc gtcgagagcg taggcggtct gcagccgggg gccctggccg
     2221 accagcgaca gccacagcac ccgcatcgac gcccccaggg gcggggcggc ggccccggcg
     2281 gccgcggcca gggcgatcat ggccgcgacc tcggccgggc tcgggccggc gacggcgacc
     2341 atgccggcca gggcggccgc gaagaccagg gccatcggag gcaggcagcg gcgctgcccg
     2401 aggcggtcga ccagccggcc ccggaccggc gccagcagcc cgctggccag ggcgatggcg
     2461 gccgaggcgg ccccggcccg ggcgagcgag ccggtcacct cctgcacgaa caggaccacg
     2521 ctcagcggcc cggtggccgt cggcagccgc ccgaccaggg aggcgagcag gagcggccgg
     2581 acgtggggga gccgcaggat ggccaggtac gaggagcgca cggcgcgatc ctacggcttc
     2641 cacccggtcg ggtggtatgc ccttcctgta ccgaacgcac ggctctgcgc ggagatccct
     2701 tgccgattag cagttcgtga gcggcaggag gtagaagggg agggatgggg aggattgccc
     2761 cgttgcggag ccgtcgagcc acaccagaaa ggagtggatt cgattgccgt cgcaccggca
     2821 cgtgagatgg tgatagttca aaagggctga tacatacgta ccccgggcgg atcagctcgg
     2881 ggtacgctag tcagaagaat cggtcgtcac ctcgccgggg aggggcagga tgtcggccag
     2941 actgcgctgg tacgtcggca gcctcaccgt ggccgccggg ttcgtgctgg ccttcctgct
     3001 gacccggctc gacctcggcc gcgcccgcga ggtcgccggg gcgatcgccg tgttcgccct
     3061 cttcatgatc gccggcgagc tggtcctcct ccggatcaac gcccagaacc ggctcaagga
     3121 ggtgtcgatc acctccacct tcgcctacgg gctggtcccc ctggccggca cggcggtggc
     3181 ggtgctggtg ttcctcctcg gctcggtcgt ctccgacctg ggccggcgca agggcgccat
     3241 caagacgctg ttcaacgcgg cccagtacgt gctcgccc
//
LOCUS       DASSWR010000218         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1224315, whole genome shotgun sequence.
ACCESSION   DASSWR010000218 DASSWR010000000
VERSION     DASSWR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224315"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..699
                     /locus_tag="VFO47_03685"
     CDS             79..699
                     /locus_tag="VFO47_03685"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET8787831.1"
                     /translation="MAVLALVLIAVWLLLVAGLRTLIQVRRTGEASASRLRDRPGSPQ
                     WWARLLSGVGFACAVAAPLAELAGLDLIPLLDRTPARVAGAVLAVLGIAATLAAQLAM
                     GSSWSPDVDPAARTALVTSGPFRLVRNPIFTATAATAIGLALLVPNLLAAAMLVAFLA
                     ALELQVRLVEEPYLHRVHGNAYAKYAARTGRFLPWIGRHRPGTGSA"
     gene            complement(1288..1599)
                     /locus_tag="VFO47_03690"
     CDS             complement(1288..1599)
                     /locus_tag="VFO47_03690"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET8787832.1"
                     /translation="MSAVWLTIILVAVASAAIKAAGPLLLGDRQLGPRATVVVASLAP
                     ALLTALVLVDTITAGRRLVVDARLAGVAAAGIALLLRAPMLLVLAVAIATTALVRLLA
                     A"
     gene            complement(1596..2273)
                     /locus_tag="VFO47_03695"
     CDS             complement(1596..2273)
                     /locus_tag="VFO47_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET8787833.1"
                     /translation="MDTDGRPADRGYAAGARAGLPFAVATVVLGVSFGVLARQLGWGV
                     AAPIICSAVVLSGSAQFAAASILGAGGGVGAATLAAVFVNARFLPMGLALAPSLVGGR
                     LRRAAEGQLVIDASWALANRGDGTFERKYLIGATVPQLSAWIGGTALGAVGGGLLADP
                     EALGLDVVFPAFFLILLAEELRDRQAVAVAVAGGLVALALVPFAPPGIPVVAACAAAL
                     LGLWRRR"
BASE COUNT          376 a          974 c          910 g          329 t
ORIGIN      
        1 gtcgactggg agactgcagc ctttgttcac gttgggcctg gtatcacgga ccaagcacgt
       61 caccaagcgg agccgccgat ggccgtcctc gccctggtcc tgatcgccgt ctggctgctg
      121 ctggtggctg gcctgcgcac cctcatccag gtccggcgca ccggtgaggc cagcgcctcc
      181 cgtcttcggg accgacccgg gtccccgcag tggtgggccc ggctgctgtc gggggtcggc
      241 ttcgcctgcg ccgtggccgc ccccctggcc gagcttgccg gcctggacct gatccccctc
      301 ctggaccgga ccccggcccg ggtcgccggc gccgtgctgg ccgtcctggg catcgccgcc
      361 accttggccg cgcagctggc catgggtagc tcctggagcc ccgacgtgga tcccgccgcc
      421 cgcaccgccc tggtcaccag cgggccgttc cggctggtcc gcaacccgat cttcaccgcc
      481 acggccgcca ccgccatcgg cctggccctg ctggttccca acctgcttgc ggcggccatg
      541 ctggtcgcgt tccttgctgc ccttgagctc caggtccgcc tggttgagga gccctacctg
      601 caccgcgtgc acggaaacgc ctatgcgaag tacgccgcac ggacaggacg cttcctgccc
      661 tggatcgggc gtcaccgccc aggcacgggg tcggcgtagg ctccgaccct catcgaggtc
      721 ctggctgagc tgcgccgctt cctatccccg tcgatggacg gccgaggtcg tgccgcccac
      781 gatctatctg ccggctgggc acacttccgc gacgaagcgg cgcggtcctc gctgttggct
      841 ggcggttcgg gacccgcgat tgtccggcgg cgcgtcccag cgtggcgatc attgccaagg
      901 tcgacgccga aggaatgacc agcatggccg gcaaggcaac aacgactgac gctgcaggcc
      961 tctgagctgg gcttttggcg cccctggcag gactcgaacc tgcaccttac ggattagaag
     1021 tgcgacacgg cccgtccgac tggtgacgct ctgaagcatc gccgcaggtc ggatcgggat
     1081 caccgtctga ccagtggcat cgcgggcggc tccgtgacta acaaccggat tgccacaggg
     1141 attgccagcg agggcggctg gcccccgccg ctactccagg tagtcgcgga ctaggaccgc
     1201 ctgagcgctg tgcaagccgc aggccaccca ggccacgagc gagcatcggg gccgcaatat
     1261 cctggagtga gaaccgaccg gccgtctcta ggcggccagc aagcgcacca gcgcggtggt
     1321 ggcgatcgcc acggccagca ccagcagcat cggcgcgcgc agcagcagcg cgatcccggc
     1381 cgcggccacg ccggccaggc gcgcgtccac caccaggcgc cgcccggcgg tgatggtgtc
     1441 caccagcacc agcgcggtca gcagcgcggg cgcgagcgag gcgaccacca cggtggcgcg
     1501 cgggccgagc tggcggtcgc cgagcagcag cggcccggcc gccttgatgg cggcgctggc
     1561 caccgcgacc aggatgatcg tcagccagac cgcgctcacc ggcgcctcca caggccgagc
     1621 agcgccgccg cgcacgccgc caccaccggg atgcccggcg gcgcgaacgg caccaaggcc
     1681 agggccacca gcccgccggc caccgccacg gccaccgcct gccggtcgcg cagctcctcg
     1741 gccagcagga tcaggaagaa cgccgggaac accacgtcca ggccgagggc ctcggggtcg
     1801 gcgagcaggc caccgcccac cgcgcccagc gccgtgccgc cgatccacgc gctgagctgg
     1861 ggtacggtcg cgccgatgag gtacttgcgc tcgaaggtgc cgtcgccgcg gttggccagg
     1921 gcccaggagg catcgatcac cagctggccc tcggccgccc ggcgcagccg gccgccgacc
     1981 agggaggggg cgagggccag ccccatcggc aggaaccggg cgttgacgaa cactgccgcc
     2041 agggtggcgg cccctacccc gccgccggcg cccaggatcg aggcggcggc gaactgcgcc
     2101 gaccccgaca gcaccaccgc cgagcagatg atcggcgcgg ccacgcccca gccgagctgg
     2161 cgggccagca ccccgaacga cacgccgagc acgacggtgg ccaccgcgaa cggcagcccg
     2221 gcgcgggcgc cggcggcgta gccccggtcg gcggggcggc cgtcggtgtc catccggtga
     2281 gtctactggc gccaccggat cctcatagtt gagacggctg cggggccgct ccggggggca
     2341 agaggtgacc ggctccttcg agcccaagcg agacccaggc aggtcaggag cttccccgta
     2401 ggagttgatg cgatccagcc ggtctgcagc cacgagctgc tgaccgccgg gctgtccgtg
     2461 cggtagatcc tgatgtggac atggcggttg ggccggccac cgcgaggaca acaggcttcc
     2521 gggcgcggag gcggcctgtc gggagccgtg cgccgggtgc accctcggca gctccaggga
     2581 gcaaccagt
//
LOCUS       DASSWR010000219         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_488931,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000219 DASSWR010000000
VERSION     DASSWR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488931"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..602
                     /locus_tag="VFO47_03700"
     CDS             132..602
                     /locus_tag="VFO47_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8787834.1"
                     /translation="MLVRGPSLAETMSDYLVRELEATANVTVRYNTEAVDGHGDGRLS
                     GLTLEDRTSRATETVPATALFILIGAEPHTGWLPDTLRRDRWGFVITGTDLLQDGQPP
                     AGWPLDRPPMPLETSLPGVFAAGDVRHGSTKRVPGAVGDGSVAIRLVHEHLTYR"
     gene            916..1422
                     /locus_tag="VFO47_03705"
     CDS             916..1422
                     /locus_tag="VFO47_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787835.1"
                     /translation="MDKVRARALLDQERGRLEQLGRAVARDHDDAVAASRPEDQVDGA
                     DRRVTEETSQALSERLRDRWAALERAEARLTAGTYGFSILSGRPIPDERLEAFPLAEL
                     TVEEAAERDQDLGGRREPESRMGPSSDPFRVFDDPAAQDDGLVEDDGSDDEPVPGANL
                     GVRTTPEP"
     gene            1725..1865
                     /locus_tag="VFO47_03710"
     CDS             1725..1865
                     /locus_tag="VFO47_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787836.1"
                     /translation="MTERVPENLTEAEVVDQPEVGGSENEGDGASEVDRWEDEGGHDR
                     AG"
BASE COUNT          326 a          710 c          735 g          281 t
ORIGIN      
        1 cgtcgccggg ccggaccggg tggtgaccct gggcgacggg accgaggtga ccagccgagc
       61 ggtgatcctg gccaccgggg tgagctggcg gcggctgggc gtgcccagcc agctacgccg
      121 cccaggtcac catgctggtc cgcgggccct cgctggccga gaccatgtcc gactacctgg
      181 tccgcgagct ggaggcgacg gccaatgtca ccgtccgcta caacaccgag gccgtcgacg
      241 gccacggcga tggccggctc tccggcctga ccctcgagga ccgcacctcg cgggcgaccg
      301 agaccgtgcc ggcgacggcg ctgttcatcc tcatcggggc cgagccccac accggctggc
      361 tccccgacac cctccggcgg gaccggtggg ggttcgtgat caccggcacc gacctgctcc
      421 aggacggcca gccgccggcc ggctggccac tggaccggcc cccgatgccg ctggagacga
      481 gcctgcccgg cgtgttcgcg gccggcgacg tccgccacgg gtcgaccaag cgggtccctg
      541 gcgcggtcgg ggacgggtcg gtcgccatcc gcctggtcca cgagcatctc acctatcgat
      601 agggacgcct ccccttgggc ggccacctgg cctgccgccg ggtcgcctcg atggcgagtt
      661 gcagcaagat ctgaagcctc atattgctgg aactgcaggt ccggccccga tatcacggcc
      721 aggtcccggg gcggtcgcat caggggaacc tggcctacca agccagtaag cgcccccgag
      781 ttctgttcgc ggcggtaggg cacgatgccg ataccatggc cgagcagctt gacccgaaga
      841 ccaccgccgt gacggcctgt gggacactgc caacaacagg aactatgctg gccaagtcgc
      901 tgatgaggag ctcgggtgga caaggtgcgg gcgcgagccc tgctggatca ggagcgtggc
      961 cgactcgagc agctgggtcg agcggtggct cgcgaccacg acgacgcggt cgccgcgtcc
     1021 aggcctgagg accaggtgga cggcgccgac cgccgggtca ccgaggagac cagccaggcc
     1081 ctgagcgagc ggctgcgcga ccggtgggcc gccctggagc gagcggaggc ccgcctgacg
     1141 gcgggcacct atggtttctc gatcctgagc ggtcgaccca ttcccgacga acggctggag
     1201 gcgttcccgc tggcggagct gaccgtcgag gaggcagccg agcgggacca ggacctgggc
     1261 gggaggcgtg aaccggagtc caggatgggc ccgtcgagcg atcccttccg ggtgttcgat
     1321 gacccggccg cccaagacga tgggctggtc gaggacgacg ggagcgatga cgagccggtt
     1381 cctggagcca acctcggcgt ccgaaccacc cccgagccct gaccccgtca aggatcggct
     1441 ccgcctggtg cgcctcgcgg ccacccagca acgttgccac ggccccggct ctctcgggtg
     1501 atgtcgcctc aggccggtgc gagggttgtc tgccggtcga cccgagctca tggacgtgca
     1561 accggttgac ctcgagctgc tcgatcttga ggtgtcggac aaccgctcgc ccgaccgcaa
     1621 gccggccaat ggccagggcg ccgaaggtac cgggcacgtc gttcctgtta cctccagggc
     1681 taccatgtgc atgccacgtg tgggtatcag gaacggggca gagcatgaca gagcgggttc
     1741 ccgagaacct gaccgaagct gaagtggttg accagccgga agtcggcggc tcggaaaacg
     1801 agggcgacgg cgcgagcgag gtcgaccgct gggaggacga gggcgggcac gaccgcgccg
     1861 gctgagcctt ccggcttgta ccggcacgcc gacaaccccc tgaccctcag ggcctgagcg
     1921 gcctgaccgt actgggccac gctgatccgg gctgctcagc cgaggcgccg acctgggtgt
     1981 tgtcgctaca gtccagccat gagagcagca gtccgcacca gctacggtcc gccggacgtg
     2041 gttcggatct ca
//
LOCUS       DASSWR010000220         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_517518,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000220 DASSWR010000000
VERSION     DASSWR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517518"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="deoA"
                     /locus_tag="VFO47_03715"
     CDS             <1..408
                     /gene="deoA"
                     /locus_tag="VFO47_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET8787837.1"
                     /translation="ERGDGLERFRQFVAAQGGDPRVADDLSILPQAPVTAEVEAPQDG
                     WLAAVDAEAVGRVAAALGAGHHRKEHDVDPAVAVELSVKLGDQVQAGQPIGRILARDE
                     QAARSASDGLLATLRWSDQPVDPPPLVHEVVGA"
     gene            575..1222
                     /locus_tag="VFO47_03720"
     CDS             575..1222
                     /locus_tag="VFO47_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET8787838.1"
                     /translation="MGSPLFLLRDEPQAFVAFLIAVIVGITFHEFSHAAVASLQGDQT
                     ARSQGRLTLNPISHLDPLGSIALVLAGIGWGRPVPVTPSNLRSRRFGGIMVGLAGPAS
                     NFVLALAGVVAFRLVYPFADTTFDVPFSLRLLYMLVTVNVILGVFNLLPIPPLDGSSL
                     LSLVLPPSRQGLLNFLDQYGIFLLLGLLILAPNLLTPIFSAITEALYGLVGLSVG"
     gene            1380..2150
                     /locus_tag="VFO47_03725"
     CDS             1380..2150
                     /locus_tag="VFO47_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HET8787839.1"
                     /translation="MDYVVRTEVFEGPFDLLLHLIARQRVDIWQVSLSRITEDYLAEV
                     RRMRELDLEVATEFLVVAATLLELKAARLLPAPDADPDEVEAVLEERDLLFARILQYR
                     AYKQVAGLFGARFAEQTAYVPRRVGAEDLLRRIVPELLVGVTPEELARLAAAALTPVP
                     PPQVTTTHIAPPRLSVAEAVTSLAHRLHDRGSSSFEELVGHQAVPIEVVIGLLAVLEL
                     YKRSLVELDQTSTFGDITVRWTGGDLVDVIDLTVEEPA"
     gene            2147..>2513
                     /locus_tag="VFO47_03730"
     CDS             2147..>2513
                     /locus_tag="VFO47_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET8787840.1"
                     /translation="MTDPTPTPAPRPERPDAEPVEETAVGMAGRGTAGGPGVDGSEGD
                     PTAWPPDEVFDAVELDDRGWTRRDLEAILLVADEPVPATALAEIVGSRLSEVEELLVD
                     LAGEYARDGRGFVLRAIAGG"
BASE COUNT          301 a          985 c          863 g          364 t
ORIGIN      
        1 gagcgcggcg acggcctgga gcgcttccgc cagttcgtgg ccgcccaggg cggcgacccc
       61 cgcgtcgccg acgacctctc gatcctgccc caggccccgg tcaccgccga ggtcgaggcg
      121 ccgcaggacg gatggctggc ggcggtcgac gccgaggccg tcggccgggt cgcggccgcc
      181 ctcggcgccg gccaccaccg caaggagcac gacgtcgacc cggccgtcgc cgtggagctc
      241 tcggtcaagc tcggcgacca ggtccaggcc ggacagccga tcggccgcat cctggcccgc
      301 gacgagcagg cggcccgctc ggccagcgac ggcctgctcg ccaccctccg ctggagcgac
      361 cagcccgtcg acccgccgcc cctcgtccac gaggtcgtcg gcgcctgacc gccggcccgg
      421 gtcgttccat ggcggccgcc ggcccgggtc gccccatcgc cgtcgccgcg cggggtgggg
      481 gagagcggca cgcatcgaag gcttccgcgc gtgctagggt tgcgccgaac ggcgtgtccc
      541 ggcgccgaaa ccccccgttc gaacgaggcg acgcatgggc tccccgctgt tcctgctccg
      601 cgacgaaccc caggcgttcg tggccttcct gatcgcggtg atcgtcggca tcaccttcca
      661 cgagttcagc cacgccgcgg tggccagcct ccagggcgac cagacggccc ggtcccaggg
      721 ccggctcacc ctcaacccga tctcccacct cgacccgctc gggtccatcg cgctggtcct
      781 ggccgggatc ggctggggcc ggcccgtgcc cgtgaccccg tcgaacctcc gcagccggcg
      841 gttcgggggg atcatggtcg gcctggccgg accggcctcc aacttcgtgc tcgccctggc
      901 cggcgtggtg gcgttccgcc tcgtctaccc gtttgccgac accaccttcg atgtcccgtt
      961 cagcttgagg ctcctctaca tgctggtcac cgtgaatgtc atcctcggcg tgttcaacct
     1021 cctgccgatc ccgccgctgg acgggtcgag cctgctctcg ctggtgctgc cgccgtcgcg
     1081 ccagggcctc ctcaacttcc tcgaccagta cgggatcttc ctgctcctcg gcctcctgat
     1141 cctggccccc aacctcctga cccccatctt cagtgccatc accgaggcgc tctacggcct
     1201 ggtcggtctc tccgtcggct gaccggccgc cccctcggct gacggccccg cggcggcccg
     1261 ccgttgtccc cgacgccgca cgctggctac catcggccgg ctgcgccgat ctcccggcac
     1321 cgctccgccg acccagcgcg tcgatccgtc gactcgacga caagtagata agtagataca
     1381 tggactatgt ggtcaggacc gaggtgttcg aggggccgtt cgacctgctc ctgcacctga
     1441 tcgcgcgcca gcgggtggac atctggcagg tgtcgctgtc ccgcatcacc gaggactacc
     1501 ttgccgaggt gcgccggatg cgcgagctcg acctcgaggt ggcgaccgag ttcctggtcg
     1561 tcgcggccac cctgctggag ctgaaggcgg cccggctgct ccccgccccg gacgccgacc
     1621 ccgacgaggt cgaggccgtc ctcgaggagc gggacctgct cttcgcccgg atcctccagt
     1681 accgggccta caagcaggtc gccggcctgt tcggggcccg cttcgccgag cagacggcct
     1741 acgtcccccg ccgggtcggc gccgaggacc tcctccgccg catcgtgccc gagctgctgg
     1801 tcggggtcac cccggaggag ctggcccgcc tggccgccgc ggccctcacc ccggtcccgc
     1861 cgccgcaggt caccaccacc cacatcgcgc cgccccggct gtcggtcgcc gaggccgtca
     1921 cctccctcgc ccaccgcctc cacgaccgcg ggtccagcag cttcgaggag ctggtcggcc
     1981 accaggcggt gccgatcgag gtggtgatcg gcctgctcgc cgtgctggag ctgtacaagc
     2041 gctcgctggt cgagctcgac cagacgtcga ccttcggcga catcaccgtc cgctggaccg
     2101 gcggcgacct ggtcgacgtc atcgacctga cggtcgagga gccggcatga ccgacccgac
     2161 cccgaccccc gccccccggc ccgagcgccc agacgccgag ccggtggagg agacggcggt
     2221 cggcatggct gggcgcggga ccgcgggagg gccgggggtg gacgggtcgg agggcgaccc
     2281 gaccgcgtgg ccgccggatg aggtgttcga cgcggtggag ctggacgacc gcgggtggac
     2341 ccggcgtgac ctggaggcga tcctgctggt ggccgacgag ccggtgccgg ccacggcgct
     2401 ggccgagatc gtcggctcgc ggctctcgga ggtggaggag ctgctggtcg acctggccgg
     2461 cgagtacgcc cgcgacgggc gcggattcgt cctgcgggcg atcgccggcg gct
//
LOCUS       DASSWR010000221         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_827696,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000221 DASSWR010000000
VERSION     DASSWR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827696"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..726
                     /locus_tag="VFO47_03735"
     CDS             97..726
                     /locus_tag="VFO47_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8787841.1"
                     /translation="MDDSVPLEIGPLDRDRLGEVGAVFAASHGRYPTFAHVFPDPARR
                     AKALRAFFTATVRDALAAGRVDAAVAGGRLLGVAVWLPPGAFPWSAGRQLRAAPAMLR
                     VLAAAPAAFPRFMRLGANAARLHPRDPHWNLETMGIVPGAQGRGVGTRLLAPGLARAD
                     QDGLACYLTTARRENLRFYGRFGFEVEHDALQLAPGGPTSWGMRRPPRT"
     gene            746..1117
                     /locus_tag="VFO47_03740"
     CDS             746..1117
                     /locus_tag="VFO47_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787842.1"
                     /translation="MPAFIKNLTVDCDDALVVARFWAAALGSDVDEDSTPERAYVEAA
                     AWGGPNIWFRGGRGPKLAGNRVHLDLRALTSMDEEVERLVGLGASVVERHAAITELRD
                     PEGNEFCVEPGPGDPHPASGR"
     gene            complement(1134..1394)
                     /locus_tag="VFO47_03745"
     CDS             complement(1134..1394)
                     /locus_tag="VFO47_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787843.1"
                     /translation="MPDRVAQRRWGLRSLAFLGLAAGSIILSNWMRMTGNTDVILLTF
                     IGTVVGLVGAAVCTVRGVRAWGGRLPREEPPGRPGGRPGDRP"
     gene            complement(1483..1917)
                     /locus_tag="VFO47_03750"
     CDS             complement(1483..1917)
                     /locus_tag="VFO47_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laminin G domain-containing protein"
                     /protein_id="HET8787844.1"
                     /translation="MPNSTALNPGSGDYAVEFRMRTTHSFGNVIQKGQAAAPGGYWKF
                     QQPSGKISCLFRGSAGSSTASAGSTVRVNDGNWHTVRCERTATMVTMIIDGVVTGRNR
                     NRTGTISNTRPVTIAGKGNCDQIEITCDYFSGDLDYVRIETT"
     gene            complement(1930..>2138)
                     /locus_tag="VFO47_03755"
     CDS             complement(1930..>2138)
                     /locus_tag="VFO47_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787845.1"
                     /translation="APAHAAFERPVAVWQMNEAAGARTMVDSSGNRRNGTIGANVQVG
                     VALSGGGTGYRFPYKRPNTPPADP"
BASE COUNT          246 a          791 c          796 g          305 t
ORIGIN      
        1 tcgctcgtcc ctccgctggt gtcgttcgtg ctctcatgac agtctcgccc acggtgcccg
       61 gtgttgcagc ccatttccag gcgggaggtg gacagcatgg acgacagcgt gccgctcgag
      121 atcggccccc tcgaccggga ccggctgggt gaggtcgggg cggtgttcgc ggccagtcac
      181 ggccgctacc cgaccttcgc ccacgtcttc cccgacccgg cccggcgggc gaaggcgctg
      241 cgggcgttct tcaccgccac cgtgcgcgac gccctcgccg ccgggcgggt cgacgccgcg
      301 gtcgccggcg ggcggctgct gggcgtggcc gtgtggctgc cgcccggcgc cttcccctgg
      361 agcgccggcc gccagctccg ggccgccccg gcgatgctcc gggtcctggc cgcggccccg
      421 gccgccttcc cgcggttcat gcgccttggc gccaacgcgg cccgcctgca cccacgcgac
      481 ccccactgga acctggagac catgggcatc gtccccggcg cccagggccg cggggtcggc
      541 acccgcctgc tcgctcccgg cctggcccgc gccgaccagg acgggctggc ctgctacctg
      601 accacggcgc ggcgcgagaa cctccgcttc tacgggcggt tcgggttcga ggtcgagcac
      661 gacgccctcc agctcgcccc cggcggcccc acgtcatggg gcatgcgccg gcccccgcgg
      721 acctgacccc agccgaggag gaccgatgcc cgcgttcatc aagaacctga ccgtggactg
      781 cgacgacgcc ctggtggtgg cgcggttctg ggcggcggcg ctggggtcgg acgtggacga
      841 ggactccacc ccggagcgcg cctacgtgga ggccgccgcc tggggcgggc ccaacatctg
      901 gttccggggc gggcgcggtc ccaagctggc cgggaaccgg gtccacctcg acctgcgcgc
      961 cctgaccagc atggacgagg aggtcgaacg gctggtcggg ctgggggcgt cggtggtgga
     1021 gcgccacgcc gccatcaccg agctgcggga ccccgagggg aacgagttct gcgtcgagcc
     1081 gggcccgggc gacccgcacc cggcgtcggg acgctgagcg ggcccggcgg cggctaggga
     1141 cggtcgccgg gccggccgcc ggggcggccg ggcggctcct ccctggggag cctgccgccc
     1201 caggcgcgga cgcccctgac ggtgcagacg gcggcgccga ccaggccgac cacggtgccg
     1261 atgaaggtga gcaggatgac gtcggtgttg ccggtcatcc gcatccagtt ggacaggatg
     1321 atcgagcccg cggccagccc gaggaacgcc aggctgcgca gcccccagcg cctctgtgcc
     1381 acccgatcgg gcatgcgacc tcctgttcgg cttcccgcag tctagcccgg gacggcgaac
     1441 agccccgggt gcaggccccg gggctgcccg tcgcgggcgc ggtcaggtcg tctcgatccg
     1501 cacgtagtcg aggtcaccgg agaagtagtc gcaggtgatc tcgatctggt cgcagttgcc
     1561 cttgccggcg atggtcaccg ggcgggtgtt ggagatggtg ccggtgcggt tgcggttccg
     1621 gccggtcacg accccgtcga tgatcatcgt gaccatcgtg gccgtgcgtt cgcaccggac
     1681 cgtgtgccag ttgccgtcgt tgacccggac cgtcgagccg gccgaggcgg tgctgctccc
     1741 ggccgagccc cggaacaggc aggagatctt cccgctgggc tgctggaact tccagtagcc
     1801 gcccggcgcc gcggcctggc ccttctggat cacgttgccg aacgaatggg tggtgcgcat
     1861 ccggaactcg accgcgtagt cgccgctgcc cgggttcagg gccgtgctgt tcggcaccct
     1921 caccaggtgc tagggatcgg ccggcggggt gttgggccgc ttgtagggga accggtaccc
     1981 ggtgcccccg cccgagagcg ccacgccgac ctgcacgttg gccccgatgg tgccgttgcg
     2041 cctgttgccg ctgctgtcga ccatggtccg ggccccggcc gcctcgttca tctgccagac
     2101 ggccaccggc cgctcgaagg cggcgtgggc gggcgcgg
//
LOCUS       DASSWR010000222         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_291133,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000222 DASSWR010000000
VERSION     DASSWR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291133"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VFO47_03760"
     CDS             complement(<1..745)
                     /locus_tag="VFO47_03760"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HET8787846.1"
                     /translation="MLATIAVFAREGLTAARTRTVWVQRVLTVLTFGAIYLVLLKTLH
                     WNLILTRTTAAGGDMGSHHYVAEFLREELLPRGRVTGWAPGWFAGIPMLTFYFPIPYV
                     LIALLTLPLGDQLAFKLVTVLGILLLPVTCWAAFRVLRLREPAPLFAACAACVFLFMS
                     QVTPTQQFTIWGGNIASTMAGEFPFSISFALLPLALAVLWRVCEDGKGWRAAALLVSA
                     VVLCHILTTIVLVLGVVVLVLRRPLAVALT"
     gene            881..2887
                     /locus_tag="VFO47_03765"
     CDS             881..2887
                     /locus_tag="VFO47_03765"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET8787847.1"
                     /translation="MPPPVRPTRPVPRWHGEWLPAADLSLDEERAATVSFDRRGRCRL
                     TLALDRPATDADLAALEDAADVLPNLHLYDLDDTADYPERHAEAFGLAEPLIPDAFNH
                     AAEVELLAPPGGRPPPRRLGRFGLPAVTAGSGVAAGSGLTAGSGVVDSARLSTSPSAT
                     LPDRGPGTAGGPRVERALLDAGRRLVAATRISASTLCGLALGLAVTVFVVAFVGATWT
                     AHARFGTYGFDVGIYDQATWLLSQGRAPFVTVRGLDLFGQQAAYIMVLVAPLYRLWAD
                     PRLLLLLQVLFLALPAPILYRLGTRRLGHPAAGLAVAVAYLAYPGVQWAISWQFHPEA
                     IAAGLLALAVAAADRRRHRAMALWLALAALCGEELGLVVAGFGLLLVVSGRRAVGWRT
                     AGAGLAWFLVATYVLMPVHAGRATRLFETDYGIAGSGPQALLAALPTMAGHALQTGLA
                     NDGLFYLLLVFLPLLGLPLLAPRWLLPVAPPLLLNLAAVQPEHHQLRFHYLATAAPLL
                     AAGAVAGLAWVRSARRPLLAPALVLLVLVAGFTSWRYGPAPWARDPVAIPAGPTDQAR
                     RAALDLVDEGAPVSAQFNLVPHLGHRVEVYEWPNPFRAVNWGVAGDEHPEATLERLRF
                     VVVQRDLLSEQDQGLLDQLQGDPAWRTAMDRDGVVVLERQEAVP"
     gene            2884..>3075
                     /locus_tag="VFO47_03770"
     CDS             2884..>3075
                     /locus_tag="VFO47_03770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8787848.1"
                     /translation="MTGGPAGAGDPAAGSTLSIVLPCLDEVGRLPGTLAAYQAHFPPS
                     RAEVELIVVDDGSTDGTSAI"
BASE COUNT          375 a         1175 c         1121 g          404 t
ORIGIN      
        1 aggtcagggc cacggccagg ggccgccgca gcaccagcac gaccacgccg aggacgagca
       61 cgatcgtggt caggatgtgg cagaggacca ccgccgagac cagcagcgcg gccgcccgcc
      121 agcccttgcc gtcctcgcac acccgccaga gcacggccag ggccagcggc agcagcgcga
      181 acgagatcga gaaggggaac tccccggcca tcgtgctggc gatgttgccg ccccagatcg
      241 tgaactgctg ggtcggggtg acctgagaca tgaacaggaa cacgcacgcc gcgcaggcgg
      301 cgaacagggg ggccggctcg cgcaggcgca gcacccggaa cgccgcccag caggtcaccg
      361 ggagcaggag gatccccagc acggtcacca gcttgaacgc cagctggtcg ccgaggggca
      421 gggtgagcag ggcgatgagc acgtagggga tcgggaagta gaaggtcaac atggggatcc
      481 cggcgaacca gccgggcgcc cagccggtca cccgcccccg cggcagcagc tcctcgcgca
      541 ggaactcggc cacgtagtgg tgcgacccca tgtcgccgcc cgccgccgtc gtccgcgtca
      601 ggataaggtt ccagtgcagg gtcttgagaa ggacgagata gatggcaccg aaggtcagca
      661 cggtgaggac gcgctgcacc cacacggttc gggtgcgggc cgcggtcagc ccctcgcggg
      721 cgaagacggc gatcgtcgcc agcactgcct cctcgggtcg tggtcgccag gtaacgaggt
      781 ttgcgcgata ccgaaacgac cgctctgaac gcctaccgca agccgtcggt ggtggtatcg
      841 cacaaacctc gttagccatg aaaccaggat ccgcatggta atgccccctc cggtccgccc
      901 cacccggccg gtgccgcggt ggcacggcga gtggctgccc gcggccgacc tgtcgctgga
      961 cgaggagcgg gcggcgaccg tctcctttga ccggcggggc cgctgccgcc tgaccctggc
     1021 cctggaccgg cccgcgaccg acgccgacct ggccgccctc gaggacgcgg ccgacgtcct
     1081 gcccaacctg cacctgtacg acctcgacga cacggccgac taccccgaac gccacgccga
     1141 ggcgttcggg ctcgccgagc cgctgatccc cgacgccttc aaccacgccg ccgaggtcga
     1201 gctgctggcc ccgccgggcg gccgcccgcc gccccggcgc ctgggccggt tcgggctgcc
     1261 cgccgtgacc gccgggtccg gcgtggccgc cgggtccggg ctgaccgccg ggtccggggt
     1321 tgtggacagc gccaggctct ccacctcgcc gtcggctacc ctcccggatc gggggcccgg
     1381 aacggcgggt gggccgcggg tcgagcgtgc cctgctcgac gccgggcgcc ggctggtcgc
     1441 cgccacccgg atctcggcgt cgaccctgtg cgggctcgcc ctcggcctgg ccgtgaccgt
     1501 cttcgtcgtc gccttcgtcg gcgccacctg gacggcccat gcccgcttcg ggacctacgg
     1561 cttcgacgtg ggcatctacg accaggcgac ctggctgctg tcgcaggggc gggcgccgtt
     1621 cgtgaccgtc cgcggcctcg acctgttcgg ccagcaggcc gcctacatca tggtcctggt
     1681 cgcgcccctg taccggctgt gggccgaccc gcgcctgctg ctgctcctcc aggtcctgtt
     1741 cctggccctg ccggcgccga tcctgtaccg gctcggcacg cgccggctcg gccacccggc
     1801 ggccggcctg gccgtggccg tggcctacct ggcctatccg ggggtccagt gggcgatcag
     1861 ctggcagttc catcccgagg ccatcgccgc cggcctgctc gccctggccg tggccgccgc
     1921 cgaccgccgc cgccaccggg ccatggcgct gtggctggcc ctggccgccc tctgcggcga
     1981 ggagctgggg ctggtcgtgg ccgggttcgg gctgctgctg gtcgtcagcg gccggcgggc
     2041 ggtcggctgg cgcacggccg gggccgggct ggcctggttc ctggtcgcca cctacgtgct
     2101 gatgccggtg catgccggcc gggcgacgcg gctgttcgag accgactacg gcatcgccgg
     2161 gagcggcccc caggcgctgc tggcggccct gcccaccatg gccggccacg ccctccagac
     2221 cggcctggcc aacgacggcc tgttctacct gctgctggtg ttcctcccgc tgctcgggct
     2281 gcccctgctg gccccgaggt ggctgctccc ggtggccccg ccgctgctgc tcaacctggc
     2341 cgcggtgcag cccgagcacc accagctccg cttccactac ctggccacgg ccgcgcccct
     2401 gctggccgcc ggggcggtgg ccgggctggc ctgggtccgc tcggcccgcc ggccgctgct
     2461 cgcgcccgcg ctggtgctgc tggtgctggt ggccggcttc accagctggc gctacgggcc
     2521 ggcgccgtgg gcccgcgacc cggtggccat cccggccggg ccgaccgacc aggcccggcg
     2581 ggcggcgctg gacctggtcg acgagggggc gccggtcagc gcccagttca acctggtccc
     2641 ccacctgggc caccgggtgg aggtgtacga gtggcccaac ccgttccggg cggtcaactg
     2701 gggcgtggcc ggcgacgagc accccgaggc gacgctggag cggctccggt tcgtggtcgt
     2761 ccagcgcgac ctgctgagcg agcaggacca ggggctgctc gaccagctgc agggcgaccc
     2821 ggcctggcgg acggccatgg accgcgacgg cgtggtcgtg ctggaacgcc aggaggccgt
     2881 gccgtgaccg gcgggccggc cggcgccggc gacccggcgg ccgggtcgac cctctcgatc
     2941 gtgctcccct gcctggacga ggtcgggcgg ctcccgggca cgctggccgc ctaccaggcc
     3001 cacttcccgc ccagccgggc cgaggtcgag ctgatcgtgg tcgacgacgg ctccaccgac
     3061 gggacctcgg ccatc
//
LOCUS       DASSWR010000223         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_998210,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000223 DASSWR010000000
VERSION     DASSWR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998210"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFO47_03775"
     CDS             complement(<1..904)
                     /locus_tag="VFO47_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787849.1"
                     /translation="MSMTIRRGRPPARPAAARPAPGMATLLAAYLAAAFLVLAVVLAV
                     APHRAPAPPAPTWDYQRERVALEGGGALNAYARWAVGTTAALTRGSIERQRDVHRLSP
                     LDFHAGEPAARGLSAWRERHRLPLEGATLAAVCALAAWGTLRRRPGRVWLAALLMLVG
                     LTVLVTHPQTATALAARAGTAIPNLVLKAGATADPSGSVATAGREGGSGVGAGAPASL
                     GAGPGTGEGPRALAAGGPRAEAVQRTVAERYWTAFVGEPLSRLQTGTTVLATAPPAAK
                     PGVLGSLRSRVTAVGDWAVGRHGPE"
     gene            1215..1985
                     /locus_tag="VFO47_03780"
     CDS             1215..1985
                     /locus_tag="VFO47_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8787850.1"
                     /translation="MDERPWYTDFFDDDYLRIFGPLLPDERTADEVNAVVERLGLAPG
                     ARLLDLCCGPGRHAVPLAQLGFRVTGLDRSRRLLGRAAARAAGQGQPVDLVAADMRRL
                     PFADASFDAVLNLFNAFGYLEDEAQDELVLAEVARVLAPGGRFLQELANREALVRDWD
                     HRRVLRNDEDLVVVQERTLDLRTSRDLARWTLIGPDGRRVTREVAIRLYTLTELAAML
                     DRAGLELLEVHGDLEGGPLELDSSFLVTLSASAGEGIS"
BASE COUNT          259 a          804 c          957 g          255 t
ORIGIN      
        1 gctcggggcc gtggcggccc acggcccagt cccccacggc cgtgacccgg gaccgcagcg
       61 acccgagcac gcccggcttg gcggcgggcg gggcggtggc cagcaccgtg gtcccggtct
      121 ggagccgcga caggggctcg cccacgaacg ccgtccagta gcgctcggcg accgtccgct
      181 gcacggcctc ggcccgcggg ccaccggcgg cgagcgcacg cggcccctcc ccggtcccgg
      241 gcccggcgcc caggctcgcc ggggcgcccg ccccgacccc ggacccaccc tcccggcccg
      301 ccgtggcgac cgacccgctg gggtcggcgg tggcgccggc cttgaggacc aggttgggga
      361 tggccgtgcc ggcgcgggcg gcgagggcgg tggcggtctg ggggtgggtg acgaggacgg
      421 tgaggccgac gagcatgagc agggcagcca gccagacccg gccggggcgg cggcggaggg
      481 tgccccaggc ggccagggcg cagacggcgg ccagggtggc gccctcgagg gggaggcggt
      541 ggcgctcgcg ccaggcggac aggccgcggg cggcgggctc gccggcgtgg aagtcgaggg
      601 ggctgaggcg gtggacgtcg cgctggcgct cgatcgaccc gcgggtgagg gcggcggtgg
      661 tgccgacggc ccagcgggcg taggcgttga gggcgccgcc gccctccagg gcgacccgct
      721 cgcgctggta gtcccaggtc ggggcgggcg gggccggggc ccggtggggc gccacggcca
      781 gcaccacggc caggaccagg aaggcggcgg ccaggtaggc ggccagcagc gtggccatgc
      841 ccggggcggg gcgggcggcg gcgggccggg ccggggggcg gcctcggcgg atggtcatgc
      901 tcacgggaag ccccctttcg aggcgtgggc gtggaagcgc aggccggtcc ggggccgggc
      961 tcgggcaggg cggggccagg ccgggcacgg gtcgggccgg gcggggagtc ggttcggtgg
     1021 gtcacgggcg ccggcggttc aggcgcaccg gtaacgggtg gagccgtgga ggcgatgcca
     1081 gggagggagg ggtgctgctg gccccatggt cggccccgcc gggaaggaga tcaaggcttg
     1141 acttcgcggg ggccgcagag gtgccctggt cgccgccgcg gatgacccgc ggcgctacga
     1201 cgaggggacg gcggttggac gagcgaccct ggtacaccga cttcttcgac gacgactacc
     1261 tgcggatctt cggcccgctg ctgcccgacg agcggaccgc ggacgaggtc aacgcggtgg
     1321 tggagcggct ggggctggct ccgggggcgc gcctgctcga cctgtgctgc ggccccggcc
     1381 gtcacgccgt ccccctggcc cagctcggct tccgggtcac cgggctcgac cgcagccggc
     1441 ggctgctggg ccgggcggcc gcccgggccg ccgggcaggg ccagccggtc gacctggtcg
     1501 cggccgacat gcgccggctg ccgttcgccg acgccagctt cgacgccgtc ctcaacctgt
     1561 tcaacgcctt cggctacctg gaggacgagg cccaggacga gctggtcctg gccgaggtgg
     1621 cccgggtcct ggcccccggc ggccgcttcc tccaggagct ggccaaccgc gaggccctgg
     1681 tgcgggactg ggaccaccgc cgcgtgctcc gcaacgacga ggacctggtg gtggtccagg
     1741 aacggaccct ggacctgcgc accagccgcg acctggcccg ctggaccctg atcggccccg
     1801 acgggcgccg ggtcacccgc gaggtcgcca tccggctgta caccctgacc gagctggcgg
     1861 ccatgctgga ccgggccggc ctggagctgc tcgaggtcca cggcgacctc gagggcgggc
     1921 cgctggagct cgactcgagc ttcctcgtga cgctgagcgc atctgccggc gagggaatca
     1981 gctgaactct tgaaccactc attcgtcgcg ctggggtgcg ggcagggggc gggatggatc
     2041 tcggcctcgg tttcgaccag gccaccatcg ccgacctggc cgagcccagg tcgtgctgcg
     2101 cctgcccggg tagccgaccg gggaacctgc cggccggaat ctaccgatcg tcggccggag
     2161 gccgactccg agccgttgtc aggcggcggc cgaccatggc gccggcgctc gtcaccagct
     2221 cgcgctggaa ccggtcggcg gcggcggtca ggtgggccag ctcgtcgggg gcgag
//
LOCUS       DASSWR010000224         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_206875,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000224 DASSWR010000000
VERSION     DASSWR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206875"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFO47_03785"
     CDS             complement(<1..308)
                     /locus_tag="VFO47_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8787851.1"
                     /translation="MSPFTGSAGFTQRWEHFRYAEEAGVATVTFDRPERLNALTFEVY
                     ADLRDLLGELPSRDGVRVLVLTGRGRGFCSGGDVRDIIGALQAMGPRELLDFTRMTGA
                     V"
     gene            complement(313..1470)
                     /locus_tag="VFO47_03790"
     CDS             complement(313..1470)
                     /locus_tag="VFO47_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8787852.1"
                     /translation="MTEGPGRPFALSAAQESLVEQVRALAREVLVPLAAAGRPGRVNR
                     ELVRALGDHGLLARLLPGDQAPGASALELCLLREALARESTEAETALALQGLGGHPIA
                     VAGSGAARARWLPGVAAGRAVAAFALSEPGAGSDAAGLELRADPDGDGFRLSGTKAWI
                     SNAPEADVYTLFARTTPGARSRGITAFAVPGDAPGLGGAPLELLAPHAIGRLELDGVR
                     VGPDQVLGRVDEGFGVAMATLDRFRPSVGAFAVGMAQAALDVAVAHAAGRQAFGRPLA
                     EFQAVAHALAEAATRTQAARLLVYDAASRHDADPAAPGVTRAAAMAKLFATETAQAVV
                     DTAVQVLGARALERGHLLEHLYREVRAPRIYEGTSEIQRTVIARDLFRALP"
     gene            complement(1608..>2694)
                     /locus_tag="VFO47_03795"
     CDS             complement(1608..>2694)
                     /locus_tag="VFO47_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787853.1"
                     /translation="ALAINAAQLLPPAWPGVLALLSLVFTTVGAFLAGRRPANAVGWL
                     LLGWGMVMAFSAFTGAYVERGLVRAPGSLPGPGWAAWAEAVVWHPAFGLLVFLLLLFP
                     HGRLPSPRWRPFAWCTVAVYLTLSVSAAVAPGAVELYYPEAAPPVRLAVGPAAEVVFG
                     VLLPAQLLLLVVALVSLVPRLRRATGEERQQIKWFVYTVVTVIVLFVAGIVVFGAGYL
                     FPLFGLIPVSVAVAVFKYRLYDIDRLINRTLVYGLLTALLIGVYAALVFLLGRLLDPA
                     TGDSALAVAASTLAVAALFQPARRRLQELVDRRFNRRRYDATRTVERFSGRLRDHVDL
                     DTLSSELLAVVDRTVQPVSASLWLRPR"
BASE COUNT          387 a         1004 c         1053 g          250 t
ORIGIN      
        1 acggccccgg tcatgcgggt gaagtcgagc agctcccggg gccccatggc ctggagggca
       61 ccgatgatgt cgcgcacgtc gccgcccgag cagaacccgc ggccgcggcc ggtcaggacc
      121 agcacccgca ccccgtcgcg gctgggcagc tcgcccagca agtcgcgcag gtcggcgtag
      181 acctcgaagg tgagggcatt gagccgctcc ggccggtcga aggtgacggt ggccaccccg
      241 gcctcctcgg cgtagcggaa gtgctcccac cgctgggtga acccggccga gccggtgaac
      301 gggctcatgg gcctacggta gcgcgcggaa caggtcgcgg gcgatcacgg tccgctggat
      361 ctcggaggtg ccctcgtaga tgcggggggc gcggacctcc cggtagaggt gctcgaggag
      421 gtggccccgc tccagggcgc gggcccccag cacctgcacg gccgtgtcga ccacggcctg
      481 ggcggtctcg gtggcgaaca gcttggccat ggccgcggcc cgggtgaccc cgggagcggc
      541 cgggtcggcg tcgtggcggc tggccgcgtc gtagaccagc agccgggccg cctgggtgcg
      601 ggtggccgcc tcggccaggg cgtgggccac ggcctggaac tcggccaggg gccggccgaa
      661 cgcctgccgc ccggcggcgt gggccacggc cacgtccagg gcggcctggg ccatgccgac
      721 ggcgaacgcg cccacgctgg gccggaaccg gtccagggtg gccatggcga ccccgaaccc
      781 ctcgtcgacc cggcccagca cctggtcggg accgacccgg accccgtcca gctccaggcg
      841 cccgatggcg tggggagcga gcagctccag cggcgccccg ccgagcccgg gggcgtcccc
      901 gggcacggcg aaggcggtga tcccccgcga ccgggccccg ggggtggtcc gggcgaacag
      961 ggtgtagacg tcggcctcgg gggcgttgga gatccaggcc ttggtcccgc tgagccggaa
     1021 gccgtccccg tcggggtcgg cccgcagctc cagcccggcc gcgtccgacc ccgcccccgg
     1081 ctccgacagc gcgaacgcgg ccaccgcccg cccggccgcc accccgggca gccaccgggc
     1141 ccgcgccgcc ccgctcccgg cgaccgcgat cgggtgcccc cccagcccct ggagggcgag
     1201 ggccgtctcg gcctcggtcg actcccgggc cagggcctcc cgcagcaggc acagctcgag
     1261 cgcggacgcc cccggcgcct ggtcgccggg cagcagccgg gccagcagcc cgtggtcgcc
     1321 cagggcccgg accagctccc ggttgacccg tcccggccgc ccggccgcgg ccagcggcac
     1381 cagcacctcc ctggccaggg cccgcacctg ctccaccagc gactcctggg cggcgctgag
     1441 ggcgaacggc cggcccggac cctcggtcac tggccctcct ccgttcccgc ggggcgcatc
     1501 ggttcctgtg cggctcccgc gcctgaaccc gacctcgccg tgcgcggccc ccggagggga
     1561 gcgtagccga acccggacca gggtgctccg ggtcgtccac agccccctca gcggggccgc
     1621 agccacagcg aggccgagac gggctggacg gtgcggtcga ccacggccag cagctcggac
     1681 gagagggtgt ccaggtcgac gtggtcgcgg aggcggccgc tgaagcgctc gacggtgcgg
     1741 gtggcgtcgt agcggcggcg gttgaaacgg cggtcgacca gctcctggag gcggcggcgg
     1801 gccggctgga acagggcggc cacggccagg gtggaggcgg ccacggccag ggccgagtcg
     1861 ccggtggccg ggtcgagcag gcggccgagg aggaacacca gggcggcgta gacgccgatc
     1921 agcagggcgg tgagcaggcc gtagaccagg gtccggttga tcaggcggtc gatgtcgtac
     1981 agccggtact tgaacacggc cacggccacc gacaccggaa tgaggccgaa cagggggaac
     2041 aggtagccgg cgccgaacac cacgatgccg gcgacgaaca gcacgatcac cgtcaccacg
     2101 gtgtacacga accacttgat ctgctggcgc tcctccccgg tggcccggcg caagcgcggc
     2161 accagcgaga ccagggcgac gacgagcagg aggagctggg cgggcagcag caccccgaag
     2221 acgacctcgg cggcggggcc gaccgccagc cgcaccggcg gggcggcctc cgggtagtag
     2281 agctcgacgg cgccgggggc gacggcggcg ctgaccgaca gcgtcaggta gacggccacc
     2341 gtgcaccagg cgaacggccg ccagcgcggc gagggcaggc ggccgtgggg gaacagcagc
     2401 agcaggaaga ccagcagccc gaacgcggga tgccagacca ccgcctcggc ccaggccgcc
     2461 cacccggggc cgggcaacga gccgggggcg cggaccagcc cgcgctcgac gtaggcgccg
     2521 gtgaaggcgc tgaacgccat caccatgccc cagccgagca gcagccagcc gacggcgttg
     2581 gcgggccggc ggccggccag gaacgccccg acggtggtga agaccaggct gagcagggcg
     2641 agcacgcccg gccaggctgg cggcaggagc tgggcggcgt tgatggccaa ggcc
//
LOCUS       DASSWR010000225         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1110718, whole genome shotgun sequence.
ACCESSION   DASSWR010000225 DASSWR010000000
VERSION     DASSWR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110718"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFO47_03800"
     CDS             <1..510
                     /locus_tag="VFO47_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787854.1"
                     /translation="IDQATYDRLLAAGEPDRTARAKAKAAYMRRAKAEARAAQAAGGA
                     GAAPAPPSEVEEAGSVETPAAAVAAPETAPAAPSAAGAPEAAEPAADARDPEARTPEG
                     EATAAGTAVDVAPGAAVAQVDAEHLEVEVDQETYDRLIEAGEPERTARAKAKAAYARK
                     LRAAQRGQR"
     gene            507..1001
                     /locus_tag="VFO47_03805"
     CDS             507..1001
                     /locus_tag="VFO47_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET8787855.1"
                     /translation="MNGQEVAFLILAVIGGLAGLLVVTSRNVVHAALYLVVALASVAG
                     VYLLLAAPFVAFVQVIIYVGAIVVLILFGIMLTRAPVGRRVVDNTLRARVGAFVVGAA
                     VFIMFTVFLAGAFGDKRIANQAATATEALGESLFRNYVLPFEAVSILLLAALVGAIVL
                     ARRD"
     gene            1021..1326
                     /gene="nuoK"
                     /locus_tag="VFO47_03810"
     CDS             1021..1326
                     /gene="nuoK"
                     /locus_tag="VFO47_03810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET8787856.1"
                     /translation="MPLMYPLVFAAFLFSAGVYGVLARRNAVMVLMSIELMLNAVNVN
                     LVAFNAYLRDQLVSGQVFALFVIAVAAAEVGIGLAIVLLIFRNRETVNIDEVDLLRW"
     gene            1345..>2306
                     /locus_tag="VFO47_03815"
     CDS             1345..>2306
                     /locus_tag="VFO47_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET8787857.1"
                     /translation="MEKLAWLVPALPLVAWAVLLLLGKRLPGGGAAIGILAVAIGWVI
                     SLAILFGVIGGADAYHVSYEWAPIGAGFDLPIGMTVDGLAAVMMVVVTTVSLFVQIYS
                     LSYMRGDERYTFFFAKLSLFTAGMLIVVLADNLLLMLFGWEIMGVCSYFLIGHYWEDV
                     ANARAAVKAFLTTRVGDLGFMAGIFVFAWAARSFEIERIIEGVETGEISSATVTLGAA
                     LLFCGAIGKSGQFPLHTWLPDAMAGPTPVSALIHAATMVAAGVFLVARMYPVFEASGS
                     VLNEIGILASITMLIAALLALVQDDIKRVLAYSTVSQLAYMMAAL"
BASE COUNT          293 a          822 c          819 g          372 t
ORIGIN      
        1 atcgaccagg ccacctatga ccgcctgctg gccgccgggg aacccgaccg cacggccagg
       61 gccaaggcca aggcggccta catgcgccgg gccaaggcgg aggcccgggc cgcccaggcg
      121 gccgggggtg ccggggccgc cccggccccg cccagcgagg tggaagaggc gggatcggtg
      181 gagaccccgg ccgcggccgt ggccgcgccg gagaccgctc ccgcggcgcc gtcggcggcg
      241 ggagcccccg aggcggccga accggcggcg gatgcgcgcg acccggaggc tcggacgccc
      301 gagggggagg cgacggcggc ggggacggcc gtggacgtgg ccccgggggc ggcggtcgcg
      361 caggtggacg cggagcatct ggaggtcgag gtcgaccagg agacctacga ccggctgatc
      421 gaggccggcg agcccgagcg gacggcccgg gccaaggcca aggccgccta cgcccgcaag
      481 ctgcgggccg cccagcgagg tcagcggtga acggccagga ggtcgccttc ctgatcctgg
      541 ccgtgatcgg cggcctggcc gggctgctgg tggtgaccag ccgcaacgtc gtccacgccg
      601 ccctgtacct ggtcgtcgcc ctggccagcg tggccggcgt gtacctgctc ctggccgccc
      661 cgttcgtggc cttcgtgcag gtcatcatct acgtgggcgc gatcgtggtc ctgatcctgt
      721 tcggcatcat gctgaccagg gcccccgtgg gccgccgggt ggtcgacaac accctgcggg
      781 cccgggtcgg cgccttcgtg gtcggggccg ccgtgttcat catgttcacg gtcttcctgg
      841 ccggcgcctt cggcgacaag cgcatcgcca accaggccgc caccgccacc gaggccctcg
      901 gcgaatcgct gttccggaac tacgtgctgc ccttcgaggc ggtcagcatc ctgctgctgg
      961 ccgccctggt cggcgccatc gtcctcgccc gccgcgactg acaaccgagg agagccagca
     1021 atgccgctga tgtacccgct ggtgttcgcc gccttcctgt tcagcgccgg ggtgtacggc
     1081 gtgctggccc gccgcaacgc ggtgatggtg ctgatgagca tcgagctgat gctgaacgcg
     1141 gtcaacgtca acctggtcgc gttcaacgcc tacctgcgcg accagctcgt ctccggccag
     1201 gtgttcgcgc tgttcgtgat cgccgtggcc gccgccgagg tcggcatcgg cctggccatc
     1261 gtgctgctga tcttccgcaa ccgggagacg gtcaacatcg acgaggtcga cctgctgcgc
     1321 tggtgacctg agaggagagc gtaggtggag aagctggcct ggctcgtgcc cgccctgccc
     1381 ctggtcgcct gggcggtcct cctgctgctg ggcaagcgcc tccccggcgg cggggcggcg
     1441 atcggcatcc tcgccgtggc catcggctgg gtgatcagcc tggccatcct gttcggggtg
     1501 atcggcgggg ccgacgccta ccacgtcagc tacgagtggg cgcccatcgg ggccggcttc
     1561 gacctgccca tcggcatgac cgtcgacggc ctggccgcgg tcatgatggt ggtggtcacc
     1621 accgtgtcgc tgttcgtcca gatctactcg ctctcctaca tgcgcgggga cgagcgctac
     1681 accttcttct tcgccaagct gtcgctgttc accgccggca tgctgatcgt cgtcctggcc
     1741 gacaacctgc tgctgatgct gttcggctgg gagatcatgg gcgtctgctc gtacttcctg
     1801 atcgggcact actgggagga cgtggccaac gcccgggccg cggtcaaggc gttcctcacc
     1861 accagggtcg gcgacctcgg cttcatggcc ggcatcttcg tgttcgcctg ggcggcccgc
     1921 agcttcgaga tcgagcggat cattgagggc gtcgagacgg gcgagatctc cagcgccacc
     1981 gtcaccctgg gcgcggccct gctgttctgc ggggccatcg gcaagtcggg ccagttcccg
     2041 ctgcacacct ggctccccga cgccatggcc ggcccgaccc cggtgtcggc cctgatccac
     2101 gccgccacca tggtcgcggc cggggtgttc ctggtcgccc gcatgtaccc ggtgttcgag
     2161 gcctccgggt cggtgctgaa cgagatcggc atcctggcca gcatcaccat gctgatcgcg
     2221 gccctgctgg ccctggtcca ggacgacatc aagcgggtgc tggcctactc gaccgtctcc
     2281 cagctggcct acatgatggc cgccct
//
LOCUS       DASSWR010000226         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1280738, whole genome shotgun sequence.
ACCESSION   DASSWR010000226 DASSWR010000000
VERSION     DASSWR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280738"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFO47_03820"
     CDS             <1..984
                     /locus_tag="VFO47_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 57"
                     /protein_id="HET8787858.1"
                     /translation="PWGHAVAPSTPTQDYRLHVRAWQHHFAAIFGLEALGRVRGFSPA
                     EMALPNHPDVAYAFVKTLNECGYGWLLVQEHSVELADGRPLERPHLPHRLVCTSSHGH
                     TAEIVAVVKTQGSDTKLVGQLQPYHEAKSLSRLELGGRLVPPLVTQIADGENGGVMMH
                     EFPPKYLQVVRECSGSRTPMLNITEYLEQLFATGLEPADLPAIQPLFQHRIWQRMAPG
                     DGPVRLADVIGRLGREDSRFHVEGGSWTNDLSWVRGYDKVLVPMERASALFHDWVLTR
                     GVPSDDTCYRKALFHLLAAETSCYRYWGEGIWTDYGAELSRRVAELLAQDR"
     gene            complement(1112..1882)
                     /locus_tag="VFO47_03825"
     CDS             complement(1112..1882)
                     /locus_tag="VFO47_03825"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET8787859.1"
                     /translation="MATTTRAVASPGRRAASEEHTGYLNYAEVAQRALLGGVPPALGS
                     LRLAVLYASASAEASIGGDFYEALETPHGVRLLVGDVRGKGLDAVRLAGVVLGSFRDA
                     AQDRPDLSQVTAALDRSVTRAIGPEDFVTAVLVQVLDDGSAIVVNCGHPAPLLVRAGR
                     AIELDPPESAPPLGLGPRPEPLRLTLALGDRILLYTDGASEARARGGFFPLQAAAAFA
                     LVSDDLEAGLESLWAELQRHVAGQLHDDIALLLIEHSS"
BASE COUNT          343 a          786 c          734 g          316 t
ORIGIN      
        1 ccatggggcc acgccgtcgc cccctccacc ccaacacagg actaccggct gcatgtgcgg
       61 gcctggcagc accacttcgc cgccatcttc ggcctggaag cgctgggccg ggtgcggggc
      121 ttctccccgg ccgagatggc cctgcccaac caccccgacg tggcctatgc gttcgtcaag
      181 accctgaacg agtgcggcta tgggtggctg ctggtccagg agcactccgt cgagctggct
      241 gacggtcggc cgctggagcg cccgcatctg ccccaccggc tggtctgcac cagctcccac
      301 ggccataccg ccgagatcgt ggccgtggtc aagacccagg gcagcgacac caagctggtc
      361 ggccagctgc agccctacca cgaggccaag agcctgtccc gccttgagct aggcggccgc
      421 ctcgtgccgc cgttggtcac ccagatcgcc gacggcgaga acggcggggt gatgatgcac
      481 gagttccccc ccaagtacct ccaggtggtc cgggaatgct cgggctcccg gacgccgatg
      541 ctgaacatca ccgagtacct ggagcagctg ttcgccaccg gcctcgagcc agccgacctg
      601 ccggcgatcc agccgctgtt ccagcaccgc atctggcagc ggatggcccc cggcgacggc
      661 cccgttcggc tcgccgacgt catcggacga ctggggcgcg aggacagccg ctttcacgtg
      721 gagggtggca gctggaccaa tgatctgtcc tgggtgcgcg gctacgacaa ggtgctggtc
      781 ccgatggagc gggccagcgc gttgttccac gactgggtcc tcacccgtgg cgttcccagc
      841 gacgacacct gctaccgcaa ggccctgttc catctgcttg cggccgagac cagctgttac
      901 cgctactggg gagagggcat ctggaccgac tacggcgccg agctatcccg ccgtgtcgcc
      961 gagctccttg cccaggaccg ctgaccgacg ccggagcctg ccacgccgca tgcccgacga
     1021 catcgccttt gcctggtccc gagcccgcac cgtcgaccgg caacctcgag cgttggcggc
     1081 cagcgtctgc ttgcggcgaa gcggggtgcc gtcagctgct gtgttcgatg agcagcagag
     1141 cgatgtcgtc gtggagctgc ccagcgacat ggcgttgcag ctcggcccac agcgactcca
     1201 gtccggcttc caggtcgtcg ctgaccagcg cgaacgccgc cgcggcctgc aacgggaaga
     1261 acccgccacg ggcacgcgcc tcgctggccc cgtcggtgta cagcaagatc cggtcgccca
     1321 gcgccagggt gagccgcagc ggctctgggc gcgggcccag ccccagcggc ggcgccgact
     1381 cgggtgggtc cagctcgatg gcccgaccgg cacggaccag cagcggcgcg ggatggccgc
     1441 agttgaccac gatcgcgctg ccgtcgtcca ggacctggac caggaccgcg gtcacgaagt
     1501 cctccgggcc gatggcgcgg gtgacgctgc ggtccaacgc cgctgtgacc tgggacaggt
     1561 cggggcggtc ctgggcggcg tcgcggaagc tgcccagcac caccccggcc agccggaccg
     1621 cgtccaggcc cttgccgcgc acgtccccga ccaaaagccg caccccgtgc ggggtctcca
     1681 gcgcctcgta gaagtccccc ccgattgacg cctcggcgct ggccgaggcg tacagcacgg
     1741 ccaggcgcag gctccccaac gccgggggca ccccgcccaa cagcgcccgc tgggcgacct
     1801 cagcgtaatt gagatagccg gtgtgctcct ctgaggcggc ccggcggccg gggctggcga
     1861 cggcccgcgt tgtggtggcc aagtgctcct caagtgccga acagtcgtcg ccccatcgcc
     1921 ggaacccatg gcggcaccca agccaggatc atccgccccg acgctttggc atgaaacttc
     1981 ggccagcagc cacacacgcg ggaggactac ctggtcgggt ggccgggggg agttgcaccc
     2041 ggaaagggct gaaaaggcaa tgattgttgc aggaagcgtt ggcgatggtt aggcttggct
     2101 caattccgcg actgtggttc ggggtcgcgt cgacatgccg tgaaaggagt tcgatgggca
     2161 ccaagaccat caccatgca
//
LOCUS       DASSWR010000227         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_404728,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000227 DASSWR010000000
VERSION     DASSWR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404728"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(84..212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(252..1178)
                     /locus_tag="VFO47_03830"
     CDS             complement(252..1178)
                     /locus_tag="VFO47_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8787860.1"
                     /translation="MVLIADDDRDIVRFVEVNLRLEGFEVITAHDGQEALGKALDLQP
                     NLILLDVMMPRMDGYEVCTKLRADGRSAHIPVIMLTAKSLSADKVLGLTAGADDYIIK
                     PFDPMELVARVKTTLRRASEMRSLSPLTGLPGNLRIEQEIASRMERGKAVAVAYADLD
                     NFKSYNDRYGFLRGDEVISLFAQVLRRAAQDAAGPDGFVGHIGGDDFVALVPPEAAET
                     FTSRVITDFDDRIPTLYDPEDASAGSIELEDRQGQLRRFPIVSVSLGIASSAQRIFAN
                     HRELVATATELKHVAKRRQGSSYALDRRTDGF"
     gene            complement(1190..1855)
                     /gene="rpe"
                     /locus_tag="VFO47_03835"
     CDS             complement(1190..1855)
                     /gene="rpe"
                     /locus_tag="VFO47_03835"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET8787861.1"
                     /translation="MIRRLAPSVLNADLAQLADQVRLVEEAADWIHLDVMDGQFVPNL
                     TLGPPVVAALRPHTGRYLDCHLMVDNPEALLPALAEAGANGVSMHVEALDDPARALAA
                     AAGHGLDAGLALKPGTPLDSVLPYLDDLDLVLPMTVEPGFGGQAFDHRVLPKIAAARR
                     AIDEAGRPVALQVDGGVNAGTLPRCLEAGADVFVVGSAIFGADDPAAAAKAFRELIDG
                     GNR"
BASE COUNT          267 a          693 c          756 g          302 t
ORIGIN      
        1 cgggtccctg ctcggctgcc accactgccc tcctgcccag ggcctggtgg aggccgcgcc
       61 gggcaggcag cccagggcgc ggccgccgcc tccttccatc cggactgtca ccgtcggccc
      121 ctgggtttca cagggtccac cccggccgcg gaggccgggg gtcgcgggct ctcaccgccg
      181 gtggggactt ccaccccgcc ccggagacgc gcgtgctacg tcaccagcag cgtacaccca
      241 gcttgacctg gctagaaccc gtcggtgcgg cggtcaaggg cgtagctgga gccctgccgg
      301 cgcttggcca cgtgcttgag ctcggtggcg gtggcgacca gctcgcggtg gttggcgaag
      361 atgcgctggg cgctggaggc gatgccgagg gagaccgaga cgatcgggaa gcggcgcagc
      421 tggccctgtc ggtcctccag ctcgatcgag ccggcgctgg cgtcctcggg gtcgtacagc
      481 gtcgggatgc ggtcgtcgaa gtcggtgatg acccggctgg tgaaggtctc ggccgcctcc
      541 ggggggacca gggcgacgaa gtcgtcgccg ccgatgtggc ccacgaaccc gtcggggccg
      601 gccgcgtcct gggcggcccg ccgcagcacc tgggcgaaca gcgagatcac ctcgtcgccg
      661 cggaggaagc cgtagcggtc gttgtaggac ttgaagttgt ccaggtcggc gtaggcgacc
      721 gcgaccgcct tgccgcgttc catgcgggag gcgatctcct gctcgatccg gaggttgccg
      781 ggcaggccgg tcagcggcga cagcgagcgc atctcgctgg cccgccgcag ggtcgtcttg
      841 acccgcgcga ccagctccat cgggtcgaac ggcttgatga tgtagtcgtc ggcgccggcg
      901 gtcaggccca gcaccttgtc ggccgagagc gacttggcgg tcagcatgat caccgggatg
      961 tgggcgctgc ggccgtcggc ccgcagcttg gtgcagacct cgtagccgtc catccgcggc
     1021 atcatcacgt ccagcaggat caggttgggc tgcaggtcga gggccttgcc gagcgcctcc
     1081 tggccgtcgt gggccgtgat cacctcgaag ccctcgaggc ggaggttgac ctcgacgaac
     1141 cggacgatgt cgcggtcgtc gtcggcgatc aggaccatct cgtccacgct tacctgttcc
     1201 ccccgtcgat cagctcccgg aacgccttgg cggcggcggc cgggtcgtcg gccccgaaga
     1261 tggccgagcc gaccacgaac acgtcggcgc ccgcctccag gcacctgggc agggtgccgg
     1321 cgttgacgcc gccgtcgacc tggagggcga ccgggcgccc ggcctcgtcg atggcccggc
     1381 gggcggcggc gatcttgggc aggacccggt ggtcgaacgc ctggcccccg aagcccggct
     1441 cgaccgtcat gggcaggacc aggtcgaggt cgtccagata cggcaggacg ctgtccaggg
     1501 gcgtgcccgg cttgagggcc agcccggcgt ccaggccgtg cccggcggcc gcggccaggg
     1561 cccgcgccgg gtcgtcgagg gcctccacgt gcatgctcac cccgttggcc ccggcctcgg
     1621 ccagggccgg cagcagcgcc tcggggttgt ccaccatcag gtggcagtcc aggtagcggc
     1681 cggtgtgggg ccgcagggcg gccaccaccg gcgggcccag tgtcaggttg gggacgaact
     1741 gtccgtccat gacgtcgagg tggatccaat cggcggcttc ttccaccagc cgaacctgat
     1801 cggcgagctg ggcgaggtcg gcgttgagca ccgacggtgc caggcgtcgg atcacggagc
     1861 tcctcgggcg tgggccacgg ttgttgcaga tggtatcggc gggcggcgag agaaggtgag
     1921 cggcgtttgg ggcttcacct taccttgaac aaccgtgcaa cgaacatgcc gtcggcgccg
     1981 tcccggtgcg gccacagctg ccgagtctcg tccaggcg
//
LOCUS       DASSWR010000228         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1026753, whole genome shotgun sequence.
ACCESSION   DASSWR010000228 DASSWR010000000
VERSION     DASSWR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026753"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..456
                     /locus_tag="VFO47_03840"
     CDS             46..456
                     /locus_tag="VFO47_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HET8787862.1"
                     /translation="MVNPEGLAPPVGFAHAVVAAPGRTVYLGGQAAQGPDGAIVGATL
                     AEQFEVATGNLVAALAAAGGAPEHLVSLHVYTTEAGVYRARLAELGAAYRRHLGRHYV
                     ATALFEVAGLFDPAAKVELVGIAVVPDPEPEEQP"
     gene            453..1622
                     /locus_tag="VFO47_03845"
     CDS             453..1622
                     /locus_tag="VFO47_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8787863.1"
                     /translation="MSPPNPLDFLDLDHLLSEEERLIRDTTRGFVADRILPEVAGWFE
                     RGELPRELAKELGDLGLLGMHLEGYGCAGTNAVSYGLACLELEAGDSGARSFVSVQGS
                     LAMFPIWKYGSEEQKRRWLPPMAAGEVIGCFGLTEPDFGSNPAGMRTSARRDGGDWVL
                     NGTKMWITNGGIADVAVVWARTDDEGVRGFLVPAGTPGFSTSDIGRKLSLRASVTSEL
                     VLEDCRLPGDAVLPEVRGMRGPLSCLNEARFGIIWGSMGAARACFQSALEYATTRVQF
                     DRPIGGFQLTQQKLADMLVELSKGTLLALHLGRMKDEGRLAPEQVSLGKLNNVREALA
                     IARSARTILGANGVTLEYPVIRHANNLESVLTYEGTSEVHSLILGQAVTGLAAFT"
     gene            complement(1615..2121)
                     /locus_tag="VFO47_03850"
     CDS             complement(1615..2121)
                     /locus_tag="VFO47_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017611137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET8787864.1"
                     /translation="MHVSAKADYAVRAAIELAAAEAGPVKGDRIAEAQQIPLKFLENI
                     LSDLRHAGYVQSQRGAEGGYWLADPPGTITLADIIRAVDGPLANVRGARPESVEYTGS
                     TSHLVEVWIAVRVSLRSVLERVTLADLVAGVLPADVKELISDADAWVSRSAGGHTPPP
                     RARRPGPR"
BASE COUNT          291 a          859 c          801 g          307 t
ORIGIN      
        1 gcgcgtggcg caggtgaggc gccaggcgcg ccgaccccgc accaggtggt gaaccccgag
       61 gggctggccc cgccggtcgg gttcgcccac gcggtcgtgg ccgcccccgg tcgcaccgtc
      121 tacctcggcg gccaggccgc ccaggggccc gacggggcga tcgtgggcgc gaccctggcc
      181 gagcagttcg aggtcgccac cggcaacctg gtcgccgccc tggccgcggc cggcggcgcc
      241 cccgagcacc tggtgtcgtt gcacgtctac accaccgagg ccggggtcta ccgggccagg
      301 ctggccgagc tgggcgccgc ctaccgccgc cacctgggcc ggcactatgt ggccacggcc
      361 ctgttcgagg tcgccgggct gttcgacccg gcggccaagg tcgagctggt cgggatcgcc
      421 gtcgtccccg acccggagcc cgaggagcag ccatgagccc cccgaacccc ctggacttcc
      481 tcgacctcga ccacctgctg tccgaggagg agcggctgat ccgcgacacc acccgcggct
      541 tcgtcgccga ccggatcctg cccgaggtgg cgggctggtt cgagcgcggc gagctgcccc
      601 gggagctggc caaggagctc ggcgacctcg gcctgctcgg catgcacctg gagggctacg
      661 gctgcgccgg caccaacgcg gtcagctacg gcctggcctg cctggagctg gaggccggcg
      721 actccggcgc ccgcagcttc gtctccgtcc agggatcgct ggccatgttc ccgatctgga
      781 agtacgggtc ggaggagcag aagcggcgct ggctgccgcc catggccgcc ggcgaggtga
      841 tcggctgctt cggcctgacc gagcccgact tcgggtccaa cccggccggg atgcgcacca
      901 gcgcccgccg cgacggcggc gactgggtcc tcaacggcac caagatgtgg atcaccaacg
      961 gcggcatcgc cgacgtggcc gtggtctggg cccgcaccga cgacgagggg gtgcggggct
     1021 tcctggtccc ggccggcacc cccggcttct ccaccagcga catcgggcgc aagctgtcgc
     1081 tgcgggcctc ggtcacctcc gagctggtcc tcgaggactg ccggctgccc ggcgacgccg
     1141 tcctgcccga ggtccgcggc atgcgcggcc ccctgtcgtg cctcaacgag gcccgcttcg
     1201 ggatcatctg ggggtcgatg ggcgcggccc gggcctgctt ccagagcgcg ctcgagtacg
     1261 ccaccacccg ggtccagttc gaccgcccca tcggcggctt ccagctcacc cagcagaagc
     1321 tggccgacat gctggtcgag ctgtccaagg gaaccctgct ggccctgcac ctgggccgga
     1381 tgaaggacga gggccgcctc gcccccgagc aggtcagcct gggcaagctc aacaacgtcc
     1441 gcgaggccct ggccatcgcc cgcagcgccc ggacgatcct gggggccaac ggcgtcaccc
     1501 tcgagtaccc ggtgatccgc cacgccaaca acctcgagtc ggtgctcacc tacgagggca
     1561 ccagcgaggt ccacagcctg atcctgggcc aggccgtgac cggcctggcc gccttcacct
     1621 aggtccgggt cggcgggccc gcgggggcgg ggtgtggccg ccggccgagc gcgacaccca
     1681 ggcgtcggcg tcgctgatca gctccttgac gtcggccggc aggaccccgg cgaccaggtc
     1741 ggccagggtg acccgctcga gcacgctgcg caggctcacc cggaccgcga tccagacctc
     1801 gaccaggtgg ctggtgctgc cggtgtactc gaccgactcc ggccgggccc cgcggacgtt
     1861 ggccagcggc ccgtccacgg cccggatgat gtcggccagg gtgatggtgc cgggggggtc
     1921 ggccagccag tagccgccct cggcgccccg ctggctctgc acgtagccgg cgtggcgcag
     1981 gtcggacagg atgttctcga ggaacttgag cgggatctgc tgggcctcgg cgatccggtc
     2041 gcccttgacc ggcccggcct cggccgcggc cagctcgatc gccgcccgga cggcgtagtc
     2101 cgcctttgct gacacatgca tgggcgcatt cttccccatg ccgccgccgc cgggaaccgc
     2161 cggccctccg caccggctca cccagatgtc gtaggcggat tcggtcgctt gacgcagcgg
     2221 cctcggcctt caccgtggca ttctccatca accgagta
//
LOCUS       DASSWR010000229         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_150906,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000229 DASSWR010000000
VERSION     DASSWR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150906"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1342
                     /locus_tag="VFO47_03855"
     CDS             35..1342
                     /locus_tag="VFO47_03855"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HET8787865.1"
                     /translation="MTAHKHLKERIRARMARTGESYTTARRHVLNALPPADYRLLGGV
                     HPDTHAIAGVLANRGLVAPHTGQPLSEAMVLGAGGGLGAGYILWEFKAHDVRTLVFGF
                     RNSWQYPDRWATKTCARLGIPAAVHETGSARRADDELRAAVGQGVPAIAWADQQLLGY
                     RHLPAWLEGHGGPPVTVYGIDEAASTVLIDDRNRAPLTVSLDALAAARARVGSYKHRL
                     LLLDAPAAELDTDRLRRAVRDGLAEQVEHLSQRSDSFSLPAFRKWARLLTDGGNAKAW
                     PKVFADRVSLFDACLSVYENVEPVGWGGGNLRGLYAQFLDEAAGLLEAPGLGKAAAAY
                     REAEAAWHAVAETAMPAGREPFAEARRLTVRLQAQVEAGDAAQEEAAATAARLWALRD
                     RWRHQFPGDTDAEALLAALAAAVTAAGEAEEAALAVLAAAVGS"
     gene            1508..1774
                     /gene="rpsO"
                     /locus_tag="VFO47_03860"
     CDS             1508..1774
                     /gene="rpsO"
                     /locus_tag="VFO47_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET8787866.1"
                     /translation="MLVKDDKTKIITEYATKEGDTGSPEVQVALLTTRINALTEHLKV
                     HKHDHHSRRGLLQMVGRRRRLLRYLQNNDVARYRALIERLGLRR"
     gene            2060..>2815
                     /locus_tag="VFO47_03865"
     CDS             2060..>2815
                     /locus_tag="VFO47_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET8787867.1"
                     /translation="MQGAVGGRELYLETGKLAKLAGGAVLIGMGETEVLVTATAAKHP
                     KEQLDFMPLTVDVEERMYAAGKIPGGFFRREGRPSETAILTARLVDRPLRPSFPKGMR
                     NEIHVVATILSTDMQNLSDVLAINGASTAVQLAGIPFSGPVGAVRLGRRGGDWIVNPT
                     YDELEECTFDMVVAGRQNEAGGVDILMVEAEATEQAIRLMEEDGAPKPTEEALGLALE
                     LAKSHIATACALQSELVRQAGAQPKEYPLYADYG"
BASE COUNT          399 a         1090 c          992 g          334 t
ORIGIN      
        1 gggcccggcc gctctgccgc gaatggagca gtccatgacc gcgcacaagc acctgaagga
       61 gcgcatccgc gcccgcatgg ccaggaccgg ggagagctac accaccgccc ggcgccacgt
      121 cctcaacgcc ctcccaccgg ccgactaccg gctgctgggc ggcgtccacc ccgacaccca
      181 cgccatcgcc ggggtgctgg ccaaccgggg cctggtcgcc ccccacaccg gccagcccct
      241 gtccgaggcc atggtgctgg gggccggcgg cgggctgggc gccggctaca tcctctggga
      301 gttcaaggcc cacgacgtgc gcacgctggt gttcgggttc cgcaactcct ggcagtatcc
      361 ggaccgctgg gccaccaaga cctgcgcccg cctgggcatc ccggcggccg tgcacgagac
      421 cggctcggcc cgcagggccg acgacgagct gcgagccgcc gtcggccagg gcgttcccgc
      481 gatcgcctgg gccgaccagc agctcctcgg ctaccggcac ctgccggcct ggctggaggg
      541 ccacggcggc cccccggtga ccgtctacgg catcgacgag gccgccagca cggtgctgat
      601 cgacgaccgc aaccgggctc cgctgacggt gtcgctggac gccctggccg cggcccgggc
      661 ccgggtcggg tcctacaagc accggctgct gctgctggac gccccggccg ccgagctgga
      721 caccgaccgg ctgcgccggg ccgtccgcga cggcctggcc gagcaggtcg agcacctctc
      781 ccagcggtcc gactcgttct cgctgcccgc cttccgcaag tgggcccggc tgctcaccga
      841 cggcggcaac gccaaggcct ggccgaaggt gttcgccgac cgggtcagcc tgttcgacgc
      901 ctgcctgtcg gtgtacgaga acgtggagcc ggtcggctgg ggcggcggca acctgcgcgg
      961 gctgtacgcc cagttcctgg acgaggccgc cgggctgctg gaggcccccg gcctcggcaa
     1021 ggccgcggcc gcctaccggg aggccgaggc cgcctggcac gcggtggccg agacggccat
     1081 gcccgccggc cgcgagccgt tcgccgaggc ccgccgcctc accgtccggc tccaggccca
     1141 ggtggaggcg ggcgacgccg cccaggagga ggcggccgcc accgccgccc ggctgtgggc
     1201 cctgcgcgac cgctggcgcc accagttccc cggcgacacc gacgccgagg ccctgctggc
     1261 cgccctggcc gccgccgtca ccgccgccgg cgaggccgag gaggccgccc tggccgtcct
     1321 ggccgccgcg gtgggctcct gagcagcggg ttccggcgcc cagccacccg cccgtgctac
     1381 cctggcgcgg gtggctgggc cgatcggccc ggcgaccgtc cgggacgcgg gacccctggc
     1441 cgcgccgtta cagcgccagc cggggccccc gggcagaatc cgcagccgtc atgggagagg
     1501 cagcagcatg ctggtcaagg acgacaagac caagatcatc accgagtacg cgaccaagga
     1561 gggcgacacc ggctcgcccg aggtccaggt ggccctgctc accacgcgca tcaacgcgct
     1621 caccgagcac ctcaaggtcc acaagcacga ccaccactcg cgtcgcgggc tcctgcagat
     1681 ggtcggccgc cgccggcggc tgctccgcta cctccagaac aacgatgtcg cccgctaccg
     1741 ggcgctcatc gagcgcctcg ggctgcgccg ctgaccgggg tggacggacc agggtgcaga
     1801 ggacggtgat gcccctggcg cgctgagcag gaccctgccg accgggatcg ccccggccga
     1861 tgtcagttgt cagtggtgag ccctggacgc cgagccacga gcatcgccca ggcctttcga
     1921 ctgagaactg gcacctcgcc cccggctccc gcccgagcag gccgctccgg ccgcgaccgc
     1981 ggccgggcat gggttcgccc cgccaggcgc ggggcggcag aggaagaggg agagatccac
     2041 atctatggca gttcatcgtg tgcaaggcgc cgtcggcggc cgcgagctct atctggagac
     2101 cggcaagctg gccaagctgg cgggcggcgc cgtgctcatc ggcatgggcg agaccgaggt
     2161 gctggtcacc gccacggcgg ccaagcatcc caaggagcag ctcgacttca tgcccctcac
     2221 cgttgacgtc gaggagcgca tgtacgccgc cggcaagatc cccggcggct tcttccgtcg
     2281 tgagggccgg ccgtccgaga cggccatcct gaccgccagg ctggtcgacc ggccgctgcg
     2341 cccctccttc cccaagggga tgcgcaacga gatccacgtg gtcgccacca tcctgtcgac
     2401 cgacatgcag aacctctcgg acgtcctggc catcaacggc gcctcgacgg ccgtgcagct
     2461 ggccgggatc ccgttctcgg gcccggtcgg ggcggtccgc ctcggccgcc ggggtggcga
     2521 ctggatcgtc aacccgacct acgacgagct cgaggagtgc accttcgaca tggtcgtggc
     2581 cggccgtcag aacgaggccg gcggggtcga catcctcatg gtcgaggccg aggcgaccga
     2641 gcaggccatc cggctgatgg aggaggacgg cgcccccaag ccgaccgagg aggccctcgg
     2701 gctcgccctc gagctggcca agagccacat cgccaccgcc tgcgcgctcc agagcgagct
     2761 ggtccgccag gccggcgccc agcccaagga gtacccgctg tacgccgact acggc
//
LOCUS       DASSWR010000230         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1138961, whole genome shotgun sequence.
ACCESSION   DASSWR010000230 DASSWR010000000
VERSION     DASSWR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138961"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..1622)
                     /locus_tag="VFO47_03870"
     CDS             complement(420..1622)
                     /locus_tag="VFO47_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET8787868.1"
                     /translation="MKWSGLIRPPQLQHTDTDSGDERTATWLELFFDLAFVLVVAELA
                     VGLRDDRTLHGVLLFAGLFSSVWWAWAGFTFYANRFDTDDVVYRLAKLGAMLAVAGLA
                     ASATDATGSLARQFAFSHAAIRLVLVALYLRAYRHVVQARGLIVVYLTAGSIAAGLWL
                     AGAMMSEPARYAAWVVAVATEVTAPVVATKRSAGLPLHLEHLPERLGLLVILVLGESI
                     AAVVVGVHETHWERATVTVAVLGFIAAVSLWWTYFDLAGAAATHTLVERAGHRSTLLH
                     DVYAYGHWPLTLGLAGAGVGLEGAILEGGQPTLTSGTRWLLCGGVALYLGALTAIQSG
                     VAGSLRSGLPWPGIGIPLVLAAGLGRGLPPAAVLAAVTVVLVGEAAAGLAKQRSGTLT
                     TGAPEEGP"
BASE COUNT          386 a          776 c          696 g          279 t
ORIGIN      
        1 accagggccg cgcccgcgcc gacggcgttg agaaggacgt tgcgctcgtc gggaccggga
       61 caccggcgac gagacgacgg gaaccgggga ccggcctcac ccaccaccaa gctggaactg
      121 atcgccatgg catagccact ggtgatcctc cccggccgcc tcctcgtggc gcatgaccgt
      181 ccccgcagta gtgagcaagc cggccaattc gactcaccag atgatcgcca tcaaggcgac
      241 cgctcggcag gtctgacgct aggttcttca cctactgggc ctcatcgggg ccacatccca
      301 ccccgagcgt cacaacaccg gtcacaagcg gtcggccaga tggtggcctg ccgctaggcg
      361 ccaaaggatg gaggcctatg gtgatgaccc gtgtcgagct ttgcgcccac gggccgcaac
      421 tacggtcctt cctcaggggc tccggtggtg agcgtgcccg agcgttgctt ggccaggccg
      481 gccgctgcct cgccgacgag gacgaccgtc acggctgcca gcacggcggc cgggggaagg
      541 ccgcgaccga ggccggcggc gaggacgagc gggatcccga tcccaggcca gggcaggccg
      601 ctgcgcagcg atccagcgac cccgctctgg atcgccgtca aggcgcccag gtagagcgcc
      661 accccgccgc acaggagcca gcgagtaccg gaggtgaggg tcggctggcc gccttccagg
      721 atcgcgcctt ccaagccgac cccggctcct gccaacccca gggtgagcgg ccagtgccca
      781 taggcataga catcatgcaa cagggtgctg cggtggccgg cccgctcgac cagggtatgg
      841 gttgctgctg ccccagccag gtcgaaatag gtccaccaca ggctcacggc ggcaataaat
      901 ccgagaaccg ccacggtgac ggtggcgcgc tcccaatgcg tctcatggac gccgacgacg
      961 accgcagcga tcgattcgcc gagcaccaag atgaccagca gccccaaccg ctccggcagg
     1021 tgctcaagat gcaacggcag gccggccgaa cgcttggtgg ccaccaccgg cgcggtcacc
     1081 tcggtcgcaa ccgcgaccac ccaggcagcg taccgggccg gttccgacat catcgccccg
     1141 gccaaccaca gcccagcggc aatgctcccg gccgtcaggt acacgacgat cagaccccgg
     1201 gcctgcacca cgtggcggta ggcacgcagg tagagggcga ccagcaccag tcggatggcg
     1261 gcatgggaaa acgcgaactg tctggccagc gacccggtcg cgtcggtggc gctggcggcc
     1321 agcccggcga cagcaagcat ggcccccaac ttggccagcc ggtacacgac atcatcggtg
     1381 tcgaagcgat tggcgtagaa cgtgaagcca gcccaggccc accacacgct gctaaacaac
     1441 ccagcgaaca gcaggacacc gtgaagcgtg cggtcgtcgc gcaacccgac cgcgagctcg
     1501 gcgacgacca gcacgaaggc cagatcaaag aacagctcca gccaggtcgc cgtgcgctcg
     1561 tccccgctgt ccgtgtcggt gtgctgcagc tgcggcggac ggatcaaccc agaccacttc
     1621 accagcgcca accccggcta atcgtcctgg ccggcggctc gctggcgtcg gtcggcggcc
     1681 tggtcacgct cgtcagcgtc atcctgggct cggtccagcg ccatcaactg tccttctgcc
     1741 tggttccagc cggcacagcc tcgcgtgccg tctggtggat ggtcacctat ccggccgcgc
     1801 aaacaggctg tcaccagtcc accctcaccg gcgggccgcg gctgcacggc gccacggcag
     1861 actggggcga gctggcagat agtggcgccg gtcgagggat actcgtgggg ggttagggat
     1921 ccaaatcccc tcagctccac cacacgacgc atcggcaggg ttcccgctcg ggccgtggcc
     1981 ccatgctcgt cacgcgagag gaccacagga ggtgccgatg gccgtccggc tcacgatcca
     2041 ttacgacaac ctggccggcc tggcccggcc gcacctgcgg ctgtggcatg cgggcgccag
     2101 ccccgtcgac ctggccccaa ccggcgcgga cgatttc
//
LOCUS       DASSWR010000231         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1337860, whole genome shotgun sequence.
ACCESSION   DASSWR010000231 DASSWR010000000
VERSION     DASSWR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337860"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFO47_03875"
     CDS             <1..655
                     /locus_tag="VFO47_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787869.1"
                     /translation="VTVGADGNLDIDLTAGVNNAKVNAIRVAPATTSPPPPPPPGDQG
                     WGQQPGYPQQPGYPQQPGYPQQPGYPQQPGYQQPGYQQQPGYQQPGGQYQQPGHPPPG
                     GQYPQSSGMAENVASGLSYGLPGITWLTGLIFFLVDRRPEVRFHAMQAIAYGVLWTLV
                     WVVRDYMPSPIRALLGLVLFAFIVGWVVLLVQGFQGRHFKLPVLGDFAEQQAGRPGL"
     gene            704..1315
                     /locus_tag="VFO47_03880"
     CDS             704..1315
                     /locus_tag="VFO47_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET8787870.1"
                     /translation="MTQGYGPQDPNQPGYGQQPPQQPYGSQPPPYGTPAQPGYGQPPA
                     QPAGGYVADPPGYYMGRTLANWPQRVGAYLIDSLIASIPVFLAVILFSGTDPGEPPST
                     GASLVTFLLYLVSFGIFIYNRCIQMGRTGQSWGKQVLNLRLVRMADGQPIGGAMAFVR
                     DLLHILDGLICLLCIGFLWPIWDARRQTLFGDKIMNTVVLSEG"
     gene            complement(1321..2196)
                     /gene="sucD"
                     /locus_tag="VFO47_03885"
     CDS             complement(1321..2196)
                     /gene="sucD"
                     /locus_tag="VFO47_03885"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET8787871.1"
                     /translation="MAILVDSQTRLVVQGLTGREGRFHALRNREYGTNLVAGVTPGKA
                     GQDVDGVPVFNTVAAAVAEAGANTSLIFVPARFAVDALYEAADAGIGVVVLITENIPV
                     LDMTRAYNYIRAKGVALVGPNCPGVISPGKANVGIIPGEITHAGPVGLVSRSGTLTYQ
                     VMYEMAAAGVGQTTCVGIGGDPVVGSSFIDVLERFQDDPETEAVVMIGEIGGDEEERA
                     AGFISERMDKPVVAYIAGFEAPPGKRMGHAGAIISGSSGTAQAKAEALEAAGVRVGRN
                     PTEAAELAVAAVKER"
     gene            complement(2200..3372)
                     /gene="sucC"
                     /locus_tag="VFO47_03890"
     CDS             complement(2200..3372)
                     /gene="sucC"
                     /locus_tag="VFO47_03890"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET8787872.1"
                     /translation="MDLFEHQAKELFAKYGVAVPRGKVAWSPEEAEAAAAEVGGTVVV
                     KAQVQIGGRGKAGGIQLAKDPAEARQQAERILGMDIRGHTVRRVLVEQASEIAAEYYV
                     AILHDRASKGFLAMASAEGGVEIEQVAEEKPEAIVKVPVNPLVGLRSYHVNQLVYGAG
                     FDERARKGAAQLLARLYEVFVAADCMLVEVNPLVLTAGGDVIALDGKVTLDDSALDRH
                     PDLEQLRDTFAVDPQEQAAKEQGLNYIKLDGYVGIIGNGAGLVMSTLDVVAQAGGKAA
                     NFLDVGGGASAETMAAGLEVILSDPKVRSVLVNIFGGITRGDLVAQGILDALDRLGDK
                     VQVPLVVRLDGTNAEEGRALLAGSPSDKVVPAATMLDAARKAVELAGAGADTKEGA"
     gene            complement(3414..3818)
                     /locus_tag="VFO47_03895"
     CDS             complement(3414..3818)
                     /locus_tag="VFO47_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET8787873.1"
                     /translation="MAQGRIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLHQT
                     PEQIVAAVVQEDADAVGLSIHSGAHMTLFPRVVELLAERGADDVLVFGGGIIPREDIV
                     LLKEQGVAEIFTPGTPTQKIVEWLRAALDRAA"
BASE COUNT          512 a         1433 c         1333 g          564 t
ORIGIN      
        1 ggtgacggtg ggggccgacg gcaacctcga catcgacctc acggccggcg tcaacaacgc
       61 caaggtcaac gccatccggg tcgcccccgc caccacctcg ccgccacccc cgccaccgcc
      121 cggcgaccag ggctggggcc agcagccggg gtacccgcag cagcctggct acccgcagca
      181 gcctggctac ccgcagcagc ccggctaccc gcagcagccc gggtaccagc aacctggcta
      241 tcagcagcag cccggctacc agcaaccagg cgggcagtac cagcaacccg gccacccgcc
      301 gccgggcggg cagtacccgc agtccagcgg gatggccgag aacgtcgcct ccgggctctc
      361 ctacgggctg cccgggatca cctggctgac cggcctgatc ttcttcctgg tcgaccggcg
      421 accggaggtc cgcttccacg ccatgcaggc gatcgcctac ggcgtgctct ggaccctggt
      481 ctgggtggtc cgggactaca tgccgagccc catccgcgcc ctgctcggcc tggtcctgtt
      541 cgcgttcatc gtcggctggg tcgtcctgct cgtccagggc ttccagggcc ggcacttcaa
      601 gctacccgtg ctcggcgact tcgccgagca gcaggccgga cgacccgggc tgtaacggcc
      661 ggaccgggcg tcgacccacc gatcacgaca aggagcaacg ctcatgaccc aggggtacgg
      721 accccaggac cccaaccagc ccggctacgg ccagcagccg ccccagcagc cctacggcag
      781 ccagccgccg ccgtacggga cgccggccca gcctggctac ggccagccgc cggcccagcc
      841 cgcgggcggc tacgtcgccg acccgcccgg ctactacatg ggacggacgc tggccaactg
      901 gccccagcgg gtcggggcct acctgatcga cagcctgatc gcctccatcc cggtgttcct
      961 ggccgtgatc ctgttcagcg ggaccgaccc gggcgagccc ccgagcaccg gggccagcct
     1021 ggtcaccttc ctgctctacc tggtctcgtt cgggatcttc atctacaacc gctgcatcca
     1081 gatgggccgg accgggcaga gctggggcaa gcaggtgctg aacctccggc tggtgcgcat
     1141 ggccgacggc cagccgatcg gcggggccat ggccttcgtc cgcgacctgc tccacatcct
     1201 ggacggcctc atctgcctgc tctgcatcgg cttcctgtgg ccgatctggg acgcccggcg
     1261 ccagaccctg ttcggcgaca agatcatgaa cacggtggtg ctgtccgagg gctgacccgg
     1321 ctagcgctcc ttgacggcgg cgacggccag ctcggccgcc tcggtggggt tgcggccgac
     1381 ccgcaccccg gcggcctcca gggcctcggc cttggcctgg gcggtgccgc tggacccgga
     1441 gatgatcgcc ccggcgtggc ccatgcgctt gcccggcggg gcctcgaacc cggcgatgta
     1501 ggcgaccacc ggcttgtcca tccgctccga gatgaagccg gccgcccgct cctcctcgtc
     1561 gccgccgatc tcgccgatca tcaccacggc ctcggtctcc gggtcgtcct ggaagcgttc
     1621 caggacgtcg atgaagctgg agccgaccac cgggtcgccg ccgatgccga cgcaggtcgt
     1681 ctggccgacc ccggccgccg ccatctcgta catcacctgg taggtcaggg tgcccgaccg
     1741 cgacaccagg ccgaccggcc cggcgtgggt gatctcgccg gggatgatgc cgacgttcgc
     1801 cttgccgggc gagatgaccc cggggcagtt cggcccgacc agggccaccc ccttggccct
     1861 gatgtagttg taggccctgg tcatgtccag cacggggatg ttctcggtga tcagcacgac
     1921 caccccgatg ccggcgtcgg cggcctcgta gagggcgtcg acggcgaagc gggcgggcac
     1981 gaagatcagc gaggtgttgg cgccggcctc ggccacggcg gcggccacgg tgttgaacac
     2041 ggggacgccg tcgacgtcct ggccggcctt gcccggggtg accccggcga ccaggttggt
     2101 cccgtactcg cggttgcgca gggcgtggaa gcggccctcg cgcccggtca ggccctgcac
     2161 gaccagccgg gtctgggagt cgacgaggat ggccatggct caggcacctt ccttcgtgtc
     2221 ggcgccggcg ccggccagct cgaccgcctt gcgggcggcg tcgagcatgg tggccgccgg
     2281 gaccaccttg tcggacggcg agccggccag cagcgcccgg ccctcctcgg cgttggtgcc
     2341 gtccaggcgc accaccaggg gcacctggac cttgtcgccg aggcggtcga gggcgtccag
     2401 gatcccctgg gcgaccaggt cgccgcgggt gatgccgccg aagatgttga ccaggacgct
     2461 gcgcaccttg gggtcggaga ggatcacctc gagcccggcg gccatggtct cggccgaggc
     2521 gcccccgccc acgtcgagga agttggccgc cttgccgccg gcctgggcga ccacgtccag
     2581 ggtgctcatg accaggccgg cgccgttgcc gatgatgccg acgtagccgt cgagcttgat
     2641 gtagttgagg ccctgctcct tggccgcctg ctcctggggg tcgacggcga aggtgtcgcg
     2701 cagctgctcc aggtcgggat ggcggtccag cgcggagtcg tcgagggtga ccttgccgtc
     2761 gagggcgatg acgtcgccgc cggcggtcag caccagcggg ttgacctcga ccagcatgca
     2821 gtcggcggcc acgaacacct cgtacagcct ggccaggagc tgggccgcgc ccttgcgggc
     2881 ccgctcgtcg aagccggcgc cgtagacgag ctggttgacg tggtagctgc gcagcccgac
     2941 cagcgggttg acgggcacct tgacgatcgc ctcgggcttc tcctcggcga cctgctcgat
     3001 ctcgaccccg ccctcggccg aggccatggc caggaagccc ttgctggccc ggtcgtggag
     3061 gatggccacg tagtactcgg cggcgatctc cgaggcctgc tcgaccagca cccgccggac
     3121 ggtgtggccg cggatgtcca tgccgaggat gcgctcggcc tgctggcggg cctcggccgg
     3181 gtccttggcc agctggatgc cgccggcctt gccgcggccg ccgatctgca cctgggcctt
     3241 gacgaccacg gtgccgccga cctcggcggc ggccgcctca gcctcctcgg gcgaccaggc
     3301 gaccttgccg cgcggcacgg ccaccccgta cttggcgaac agctccttgg cctgatgctc
     3361 gaacaggtcc atgcgtcagc ctctccgtcg ggttgcgctg gcggtgcctc cggtcaggcg
     3421 gcccggtcga gggcggcccg cagccactcg acgatcttct gggtcggggt gcccggggtg
     3481 aagatctcgg ccacgccctg ctccttgagc aggacgatgt cctcgcgggg gatgatgccg
     3541 ccgccgaaca cgagcacgtc gtcggcaccg cgctcggcca gcagctcgac cacgcggggg
     3601 aacagggtca tgtgggcgcc cgagtggatc gagagcccga cggcgtcggc gtcctcctgg
     3661 accacggcgg ccacgatctg ctcgggggtc tggtgcaggc cggtgtagat gacctccatg
     3721 ccggcgtccc gcagggccct ggccacgacc ttggccccgc ggtcgtgccc gtccaggcct
     3781 ggcttggcga ccacgacccg gatgcgtccc tgcgccacgg tgccagctgc ccctttcgtg
     3841 tc
//
LOCUS       DASSWR010000232         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_517893,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000232 DASSWR010000000
VERSION     DASSWR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517893"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..299)
                     /locus_tag="VFO47_03900"
                     /pseudo
     CDS             complement(1..299)
                     /locus_tag="VFO47_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
     gene            283..1068
                     /locus_tag="VFO47_03905"
                     /pseudo
     CDS             283..1068
                     /locus_tag="VFO47_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011206995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
     gene            1065..2081
                     /gene="dmpG"
                     /locus_tag="VFO47_03910"
     CDS             1065..2081
                     /gene="dmpG"
                     /locus_tag="VFO47_03910"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HET8787874.1"
                     /translation="MTGTAFRLTDSTLRDGSHALSHRYTAEQVSSIVRGLDAAGVPVI
                     EVSHGDGLGGSSFNYGFSGTDERELVSAAVAAATTARIAVLLLPGIGVADDLRDMADR
                     GVQVARIATHCTEADISSQHIRLAKRLGLEAVGFLMMAHMAEPDELLAQARLMESYGA
                     DCVYVVDSAGAMTTADARARVGTLAEGLGCQVGHHAHNNLSLAVANSLAALDVGADQL
                     DGCCAGLGAGAGNCPTEVLVATCDKLGLATGVDPLEAMGVAEDLVRPLRPEMGVIDRA
                     GLLLGYAGVYGSFLLHAQRAAARYGVDVGEILLELGRRRVVGGQEDMILDVALELARQ
                     EAKA"
     gene            2078..2857
                     /locus_tag="VFO47_03915"
     CDS             2078..2857
                     /locus_tag="VFO47_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8787875.1"
                     /translation="MNAGPDELARELAGRRQDRGAGAGLTAEHPGLDLELAYAVQEAG
                     VRLLAAAGERPVGFKLGLTSRAKQAQMGVDEPLYGELTDAMLLDVGEPLDTGQLIQPR
                     VEPEIAFLLGADLAGERVGAAQVLAATAAVAPAIDVLDSRYAGYAFTLPDVVADNASA
                     ARLVLGGVPRAVDGIDLRLVGCVLEQGGRVRATAAGAAALGHPAAAVAWLVRALARRG
                     RGLHAGDVVLSGGLTEAVPVAPGDVVVARLGRLGTVELAVR"
     gene            2874..3095
                     /locus_tag="VFO47_03920"
     CDS             2874..3095
                     /locus_tag="VFO47_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET8787876.1"
                     /translation="MPFVRIDLVRGRDKAVLMRLVEEVSEVVARTLDTPIDRVRVLVS
                     EVDPDLWGIGGVPYSVARGPVDPGQAREP"
     gene            3092..3475
                     /locus_tag="VFO47_03925"
     CDS             3092..3475
                     /locus_tag="VFO47_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8787877.1"
                     /translation="MTAPPVEVAAGRVEELPAGTVRLLEAAGRRVAVGNAGGELFALD
                     DTCLHRGGSLAGGHLDGETLVCPMHWWRYDVRSGRRLGQPGLRVARYPVRVVDGEVRV
                     TVPPAAPPRSWREILLEHAREGSRG"
     gene            3472..4128
                     /locus_tag="VFO47_03930"
     CDS             3472..4128
                     /locus_tag="VFO47_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8787878.1"
                     /translation="MKAVVLDFGGPVLLTPFERVAALERSLRVAPGTFAWPGPFDPAA
                     DPRWRAMQAGELTERAYWAARAAEVAAVSGEPGVPAMMARLFDGPEEALVRPEARDLV
                     AAAQAAGLETAVLTNDLYDFHDPAWIDRMGILAEVGCVVDASRLGTRKPEPAAYRLVL
                     DRLGVAADQAVFVDDQPANAAGAEAAGLRAVWFDVTRPAASYETVRHALGLATDGGTP
                     "
     gene            4125..5561
                     /locus_tag="VFO47_03935"
     CDS             4125..5561
                     /locus_tag="VFO47_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET8787879.1"
                     /translation="MSALVEIPHVIDGEERPAADGRTFESVNPATETAWARVARGSVA
                     DAGVAVAAARRAFDDGPWPRMAPAERGRALHRLADLIEAEAATLGRLDSTDMGKPIRQ
                     ARDNDAHRAALNFRFFADYAALATDEALPSPTGHHVYTRHEPVGVAAAISPWNFPLML
                     ASWKVAPALAFGNTVVLKPAEQSPASCHRLGLLAGQAGLPPGTLNVVQGFGPGEAGQA
                     LTEDPGVDLITFTGESATGRAILAAGAPTLKRVSFELGGKGANLVFADADLANAVDWS
                     LRAIFLNAGQVCLSGSRLYVQRPVYEEFLERFVAGAEAMRLGDPLDEATEVGPLSSHE
                     HWRKVTGYLDLAATEGAKVRTGGRGDGWWVRPTVLVDVRQDMRVCREEIFGPVVVVQP
                     FDSDEEAVALANDSAYGLNAMVFTESLRRAHRVAAAVRAGTIWVNCFFVRDLRAPFGG
                     YKDSGIGREGGSWSRDFFTEAKAVVMQL"
BASE COUNT          643 a         2155 c         2053 g          711 t
ORIGIN      
        1 ggcgtcgagt agcgcgtctt ggcgatggtc tcgtcgacct tgtggccggg tagctgcccg
       61 ccctcgcccg gcttggcgcc ctgcgcgatc ttgatctgca gctcgtcggc gttgacgaga
      121 tcccagctgg tcacgccgaa gcgccccgac gccacctgct tgatcgcgct gcgctgcgag
      181 tcgccgctgg gcagcggggc gaagcggcgc ggatcctcgc cgccctcgcc ggtgttgctc
      241 ttgccgccga tgcggttcat cgcgacggcc agcgtctggt gggcgtcgag cgagatcgac
      301 ctggccttcg aggcgaccag cgcccgggtg cacgccgcca acgcgccccg gctggccgcc
      361 gccgggatca tcgccatcga cctgaccccg gccaagctgg ggccggcggt cgtgcccggg
      421 gtcaacctcg accggcacct ggacgccccc aacctcaacc tgatcacctg cggcggccag
      481 gccacggcgc cgatcgtggc cgcggtgtcc tcggccagcc cggtccccta cgccgagatc
      541 gtctccacgg tggcctcccg ctcggccggg cccggcaccc gccagaacat cgacgagttc
      601 acccgcacca ccgcccgctc cctggaggag gtcggcggcg ccggccacgg caaggcgatc
      661 atcatcctca acccggccga cccgccgatc ctgatgcgca acaccgtgtt ctgcgccctg
      721 cccgacggct acgacgaggc ggcggtgggc gccgccgtcg aggacatggt cgagcgggtg
      781 gcccggtacg tgcccggcta ccggctcaag accccgccgg tgttcgagga ggggccgttc
      841 gccaccccgg gcgggccggc cgcggcccgg gtggcggccc tgctggaggt cgagggggcc
      901 ggcgactacc tgcccccgta cgccggcaac ctcgacatca tgaccgccgc cgccgtccgg
      961 gtcggcgagg ccatcgccga caacggccca gccggcaggg aatctggttc gtcaccatcc
     1021 gatggggcca gggaagctcg ggtgggtcag cggggggtga cggcgtgacc ggcacggcgt
     1081 tccggctgac cgactcgacc ctgcgcgacg gctcccacgc cctgtcccac cgctacacgg
     1141 ccgagcaggt cagctcgatc gtccgcggcc tggacgccgc cggggtgccg gtgatcgagg
     1201 tcagccacgg cgacgggctg ggcggctcgt cgttcaacta cggcttctcc ggcaccgacg
     1261 agcgcgagct ggtctcggcg gcggtcgccg ccgccaccac ggcccggatc gcggtcctgc
     1321 tgctgcccgg gatcggggtc gccgacgacc tgcgcgacat ggccgaccgg ggcgtccagg
     1381 tggcccggat cgccacccac tgcaccgagg ccgacatctc ctcccagcac atccgcctgg
     1441 ccaagcggct cggcctggag gcggtcgggt tcctgatgat ggcccacatg gccgaaccgg
     1501 acgagctgct ggcccaggcc cggctgatgg agtcctacgg cgccgactgc gtctacgtgg
     1561 tcgactcggc cggggccatg accaccgccg acgcccgcgc ccgggtgggg accctggccg
     1621 agggcctcgg ctgccaggtc ggccaccacg cccacaacaa cctgtccctg gccgtggcca
     1681 actccctggc cgccctcgac gtcggcgccg accagctcga cggctgctgc gccggcctgg
     1741 gagcgggcgc cgggaactgc ccgaccgagg tgctggtggc cacctgcgac aagctcggcc
     1801 tggccaccgg ggtcgacccg ctggaggcca tgggcgtggc cgaggacctg gtccgccccc
     1861 tgcggcccga gatgggggtg atcgaccggg ccggcctgct gctcggctac gccggggtgt
     1921 acggctcgtt cctgctccac gcccagcggg ccgcggcccg ctacggggtc gacgtcggcg
     1981 agatcctgct cgagctgggc cgccgccgcg tcgtcggcgg ccaggaggac atgatcctcg
     2041 acgtcgccct ggagctggcc cgccaggagg cgaaggcgtg aacgcgggcc ccgacgagct
     2101 ggcccgggag ctggccgggc ggcgccagga ccgcggcgcc ggcgccggcc tgacggccga
     2161 gcaccccggg ctcgacctgg agctcgccta cgccgtccag gaggccgggg tgcggctgct
     2221 ggccgcggcg ggggagcggc cggtcggctt caagctcggc ctgaccagcc gggccaagca
     2281 ggcccagatg ggcgtggacg agccgctgta cggcgagctg accgacgcca tgctgctcga
     2341 cgtgggcgaa cccctcgaca ccggccagct gatccagccc cgggtcgagc ccgagatcgc
     2401 cttcctcctc ggggccgacc tggccgggga acgggtcggg gccgcccagg tgctggccgc
     2461 caccgccgcc gtggccccgg ccatcgacgt gctcgactcg cgctacgccg gctacgcctt
     2521 caccctcccc gacgtggtcg ccgacaacgc ctcggccgcc cggctggtcc tcggtggggt
     2581 cccccgggcc gtcgacggca tcgacctgcg cctggtcggc tgcgtcctgg agcagggcgg
     2641 gcgggtccgg gcgaccgccg ccggggcggc cgccctcggc caccccgccg ccgccgtggc
     2701 ctggctggtc cgggccctgg cccgccgggg ccgcggcctt cacgccgggg acgtcgtgct
     2761 gtccggcggg ctcaccgagg ccgtgccggt ggcgcccggc gacgtggtcg tggcccgact
     2821 cggccggctc gggaccgtgg agctggccgt ccgctgacct tcgaggagga cccatgccgt
     2881 tcgtccgcat cgacctcgtc cgcggccgcg acaaggcggt gctgatgcgg ctggtcgagg
     2941 aggtgtccga ggtcgtcgcc cgcaccctcg acacccccat cgaccgggtg cgggtgctgg
     3001 tctccgaggt cgaccccgac ctgtggggca tcggcggcgt gccctactcg gtggccaggg
     3061 gcccggtcga ccccggccag gcgcgggagc catgaccgcc ccgccggtcg aggtggccgc
     3121 gggccgggtc gaggagctcc cggccgggac ggtgcggctg ctggaggcgg ccggccgccg
     3181 ggtggccgtc ggcaacgccg gcggcgagct gttcgccctc gacgacacct gcctgcaccg
     3241 gggcgggtcg ctggccggcg gccacctcga cggcgagacg ctggtctgcc cgatgcactg
     3301 gtggcgctac gacgtccgca gcggccgccg cctcggccag cccgggctgc gcgtggcccg
     3361 ctacccggtc cgggtggtcg acggcgaggt ccgggtcacc gtccccccgg cggccccgcc
     3421 ccgctcctgg cgcgagatcc tcctggagca cgcccgcgag gggagcaggg gatgaaggcc
     3481 gtggtgctcg acttcggcgg gccggtcctg ctgaccccgt tcgagcgggt ggccgccctg
     3541 gagcggtcgc tgcgggtggc cccggggacc ttcgcctggc cggggccgtt cgacccggcc
     3601 gccgacccgc gctggcgggc catgcaggcg ggggagctga ccgagcgggc ctactgggcg
     3661 gcccgggcgg ccgaggtggc ggcggtgtcg ggcgagccag gggtgccggc catgatggcc
     3721 cggctgttcg acggccccga ggaggcgctg gtccggcccg aggcccgcga cctggtcgct
     3781 gccgcccagg cggccgggct ggagacggcc gtgctcacca acgacctgta cgacttccac
     3841 gacccggcct ggatcgaccg catgggcatc ctggccgagg tcggctgcgt ggtcgacgcc
     3901 tcccgcctcg ggacccgcaa gcccgagccg gccgcctacc ggctggtcct cgaccggctc
     3961 ggggtcgccg ccgaccaggc cgtgttcgtc gacgaccagc cggccaacgc cgccggcgcc
     4021 gaggcggccg gcctgcgggc cgtctggttc gacgtcaccc ggcccgccgc cagctacgag
     4081 acggtccgcc acgccctcgg gctggccacc gatggaggga caccatgagc gcgctggtcg
     4141 agatcccgca cgtgatcgac ggggaggagc ggccggcggc cgacggccgc accttcgagt
     4201 cggtcaaccc ggccaccgag acggcctggg cgcgggtcgc ccgcgggtcg gttgcggacg
     4261 ccggcgtggc cgttgccgcc gcccgccgag ccttcgacga cggcccatgg ccgcggatgg
     4321 ccccggccga gcgcggccgg gccctgcacc ggctggccga cctgatcgag gccgaggcgg
     4381 ccaccctcgg ccggctcgac tcgaccgaca tgggcaagcc gatccgccag gcccgcgaca
     4441 acgacgccca ccgggccgcc ctcaacttcc gcttcttcgc cgactacgcc gccctggcca
     4501 ccgacgaggc cctgccctcg ccgaccggcc accacgtcta cacccggcac gagccggtcg
     4561 gggtggccgc cgccatctca ccctggaact tcccgctcat gctggccagc tggaaggtcg
     4621 ccccggccct ggccttcggc aacacggtcg tgctcaagcc ggccgagcag tcgccggcgt
     4681 cctgccaccg cctcggcctg ctggccggcc aggccggcct gccgccgggg accctcaacg
     4741 tcgtccaggg gttcggcccc ggcgaggccg gccaggccct caccgaggac ccgggggtcg
     4801 acctgatcac cttcaccggc gagtcggcca ccggccgggc catcctggcc gccggcgccc
     4861 ccaccctcaa gcgggtcagc ttcgagctcg gcggcaaggg cgccaacctg gtcttcgccg
     4921 acgccgacct ggccaacgcc gtcgactggt cgctgcgggc catcttcctc aacgccggcc
     4981 aggtgtgcct gtccgggtcg cggctgtacg tgcagcgtcc cgtctacgag gagttcctgg
     5041 agcggttcgt ggccggcgcc gaggccatgc gcctgggcga cccgctggac gaggccaccg
     5101 aggtcgggcc cctctcctcc cacgagcact ggcgcaaggt caccggctac ctcgacctgg
     5161 ccgccaccga gggggccaag gtccgcaccg gtggccgcgg cgacggctgg tgggtccggc
     5221 ccaccgtgct ggtcgacgtc cgccaggaca tgcgcgtctg ccgcgaggag atcttcggcc
     5281 cggtggtggt cgtgcagccc ttcgacagcg acgaggaggc ggtggccctg gccaacgact
     5341 cggcctacgg cctcaacgcc atggtgttca ccgagagcct gcgccgcgcc caccgggtcg
     5401 cggccgccgt ccgggccggc accatctggg tcaactgctt cttcgtccgc gacctgcggg
     5461 cgcccttcgg cggctacaag gactccggca tcggccgcga gggcgggagc tggagccgcg
     5521 acttcttcac cgaggccaag gccgtggtca tgcagttgtg ac
//
LOCUS       DASSWR010000233         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_263591,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000233 DASSWR010000000
VERSION     DASSWR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263591"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFO47_03940"
     CDS             complement(<1..212)
                     /locus_tag="VFO47_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8787880.1"
                     /translation="MPASARSDGGVEMTRPFDGRLAGTTALITGASQGLGRAVALAYA
                     REGADLVLVARRPGPLEGVRDEAEALG"
     gene            357..1265
                     /locus_tag="VFO47_03945"
     CDS             357..1265
                     /locus_tag="VFO47_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8787881.1"
                     /translation="MPVRRLFLLAFIWGWSFLFIKVILEDAPPTFLAWGRIALGLAVL
                     AVAMRHRSERLPERRYWGHLLVLGLAMSVVPFILIGWGEEHISSALASVLNACTPLFA
                     AGFAAGLLGERLRPPQLAGIAVGFLGVAVVAGVGGGDLAGSSLLGSAAVVLAGAGYGF
                     GFAYANRFTTGLSALQLSFGQLLAATVVLAPVAAVDVAAGRVDLGPLAALCLVLLGAL
                     GTGYAYLLNYRTLQESGATVASLVTYLVPLVAVAAGVVVLGEPFSLRLVVGGLIVVLG
                     VALVQGRLFGPRERATEPVPETATCD"
     gene            complement(1279..1677)
                     /locus_tag="VFO47_03950"
     CDS             complement(1279..1677)
                     /locus_tag="VFO47_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787882.1"
                     /translation="MDTTSHPDVTAFLDRYAEALTGGDLPGIAACYMVPGLVVGDAAA
                     IPVAEPGEVEAAFAGAAEAYRAQGLVGIRPELRAVDPLTPALTLADVRWAYLDESGAA
                     RQHTSYRYLLRRSDSGELAIQVVVDTTPQA"
     gene            1742..2128
                     /locus_tag="VFO47_03955"
     CDS             1742..2128
                     /locus_tag="VFO47_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017610105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET8787883.1"
                     /translation="MTTRPVAPTQPHLARLRVERQHRLIEELRLAAGRHATAEDLATA
                     LGVDVRTVERDITRLRDAGVRITARRGPGGGFRLDVPPSVPPVHLSPGEIAALVVSVT
                     AVGPYVSATARSALTRLLDALQPPQR"
     gene            complement(2088..2552)
                     /locus_tag="VFO47_03960"
     CDS             complement(2088..2552)
                     /locus_tag="VFO47_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HET8787884.1"
                     /translation="MVAAVYAVVSFYWAFGGTAGIGTLGGRLEELGRVGDPAVLWLAG
                     VAGVLKAAGAVLALALVRPWGRVLPRRVLLMVAWAGAVVLVGYGGLYVVGGALALGGV
                     GEVAGAGDRAALWWHVLVWDLWFLVWGVLLGVAAWRFGRLSAAVAGGRRAGG"
     gene            2663..2736
                     /locus_tag="VFO47_03965"
     tRNA            2663..2736
                     /locus_tag="VFO47_03965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2697..2699,aa:Ala,seq:cgc)
BASE COUNT          349 a         1214 c          944 g          370 t
ORIGIN      
        1 cccagcgcct cggcctcgtc ccggaccccc tccagcggcc cgggccgccg ggccaccagg
       61 accaggtcag ctccctcgcg ggcgtaggcc agcgcgaccg cccgccccag cccctgggag
      121 gcgccggtga tgagcgcggt cgtcccggcc agcctgccgt cgaaaggtcg ggtcatctcg
      181 acacccccgt cgctgcgagc agatgccggc atggtgcgcc cgcccccacg aatttgtcaa
      241 cgcgtctgtt gacttaatcg cgaggtggca ccggggctcc tgaaaccggc ccgccgggaa
      301 cggtgttgga gcgggagtag cctgggaggg actctccctt ccgaccttcc tgcggggtgc
      361 ccgtgcgacg cctgttcctg ctggccttca tctggggctg gtcgttcctc ttcatcaagg
      421 tcatcctcga ggacgccccg cccacgttcc tggcctgggg acggatcgcc ctcggcctgg
      481 ccgtgctggc cgtcgccatg cgccaccgct ccgagcgcct gcccgagcgc cgctactggg
      541 gccacctgct ggtcctgggg ctggccatga gcgtggtgcc gttcatcctg atcggctggg
      601 gcgaggagca catcagctcg gccctggcct cggtcctgaa cgcctgcacg ccgctgttcg
      661 cggccgggtt cgcggccggc ctgctcggcg agcgcctgcg cccgccccag ctggccggca
      721 tcgccgtcgg cttcctgggc gtggccgtgg tcgccggggt cggcggcggc gacctcgccg
      781 gcagctccct gctcggctcg gccgcggtcg tcctcgccgg cgcgggctac ggcttcgggt
      841 tcgcctacgc caaccgcttc accaccggcc tgtcggccct ccagctgtcg ttcggccagc
      901 tcctggccgc caccgtggtg ctggcgccgg tggccgccgt ggacgtcgcc gccggccggg
      961 tcgacctcgg ccccctcgcc gccctctgcc tggtcctgct gggcgccctc ggcaccggct
     1021 acgcctacct gctcaactac cgcaccctcc aggagagcgg cgccaccgtc gcctccctgg
     1081 tcacctacct cgtccccctg gtcgccgtcg ccgccggggt ggtcgtcctg ggcgagccgt
     1141 tctccctccg cctcgtcgtc ggcggcctga tcgtggtcct gggcgtcgcc ctggtccagg
     1201 gccgcctgtt cggcccccgc gagcgggcca cggagcccgt cccggagacc gcgacctgcg
     1261 actgagcggc ccggccgctc aggcctgggg ggtggtgtcg acgaccacct ggatggcgag
     1321 ctcgccgctg tcggagcggc gcagcaggta gcggtaggag gtgtgctggc gggcggcgcc
     1381 ggactcgtcc aggtaggccc agcggacgtc ggccagggtc agggcggggg tgagggggtc
     1441 gacggcccgc agctcggggc ggatgccgac caggccctgg gcgcggtagg cctcggcggc
     1501 gccggcgaag gcggcctcga cctcgcccgg ctcggcgacc gggatggcgg cggcgtcccc
     1561 gacgaccagt ccgggaacca tgtagcaggc ggcgatgccc ggcaggtccc cgccggtgag
     1621 ggcctcggcg tagcggtcga ggaaggccgt cacgtcggga tgcgatgtcg tgtccatgga
     1681 cccgactcta cgatgagagc ccggcacgcc tgggccggtc ttcccagaaa ggccccgacc
     1741 catgacgaca cggcccgtcg cgccgaccca gccgcacctg gccaggctcc gggtcgagcg
     1801 ccagcaccgc ctgatcgagg agctgcgcct ggccgccggc cgccacgcca ccgccgagga
     1861 cctggcgacc gccctcgggg tcgacgtccg caccgtcgag cgcgacatca cccgcctccg
     1921 cgacgccggc gtccgcatca ccgcccgccg cggccccggc ggcggcttcc gcctcgacgt
     1981 ccccccgtcg gtccccccgg tccacctctc ccccggcgag atcgccgccc tggtcgtctc
     2041 cgtcaccgcc gtcggcccct acgtctcggc caccgcccgc tcggccctca cccgcctgct
     2101 cgacgccctc cagccaccgc agcgctgagc cgcccgaagc gccaggccgc caccccgagc
     2161 agcacccccc acaccaggaa ccagagatcc cagaccagca catgccacca cagcgccgcc
     2221 cggtccccgg cccccgccac ctcacccacc ccgcccagcg ccagcgcccc cccgaccacg
     2281 tacagcccgc cgtaccccac cagcaccacc gccccggccc acgccaccat cagcagcacc
     2341 cgccgcggca gcacccgccc ccacggccgc accagcgcca gcgccagcac cgcccccgcc
     2401 gccttcagca ccccggcgac cccggccagc cacagcaccg ccggatcgcc gaccctcccc
     2461 agctcctcca gccgcccccc gagcgtgccg atcccggccg tccccccgaa cgcccagtag
     2521 aagctgacca ccgcatagac cgccgccacc acgcacgcgg cgtaggcggg ccagttcgcg
     2581 gtacgttcgc aagccctcat gacgccaggg taggtggatc gggccaccga accggtctgg
     2641 taccatccca aacgctcacc tagggggtgt agctcagccc ggcagagcgc ctccctcgca
     2701 cggaggaagc caggggttca aatcccctca cctccacccc caacaccgca ggtcagagcg
     2761 tcgccggtca cacatcggcg gcgctctcgt cgttccccgg accacctggg gccacactgg
     2821 ggccacggcc ggcctgcctg tgcagccgaa cgacggtgcc ctcgggggcc ggctggg
//
LOCUS       DASSWR010000234         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_179163,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000234 DASSWR010000000
VERSION     DASSWR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179163"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFO47_03970"
     CDS             <1..484
                     /locus_tag="VFO47_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET8787885.1"
                     /translation="VGGTVATLLANRLKSLEAIAAASEEELNDVGGVGPTIAASVAAW
                     FADEGNQDLVRRLVEAGLNTKADGGGGADLPQTLAGMSFVLTGGLDGFTRDEATRAIE
                     DRGGRVASSVSKKTAYVVVGADPGSKAARAAELGVPTLDEAAFAALLAKEEVEEVADG
                     "
     gene            477..1067
                     /locus_tag="VFO47_03975"
     CDS             477..1067
                     /locus_tag="VFO47_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787886.1"
                     /translation="MGDVWHPLDPEGSRPQQPSGRDRGPDPEPIVRTVRVAFLFAGMV
                     VAVMVSAGLNSLGGPGAVATPVGGVAVTAVVAVAVLAVLGGAGLALRPARLLVAGKRA
                     LEHPDPRLRRPRAERARAIGFRGLAMGWASQALVFGLLPAFAGLVLELLYDRQWELLA
                     FAGISLLAGFVFQLQVAGAVRLAVDDPELRASYGSR"
     gene            1158..1403
                     /locus_tag="VFO47_03980"
     CDS             1158..1403
                     /locus_tag="VFO47_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HET8787887.1"
                     /translation="MSIGASIFLLVIGAILTFAVNVTTEGFNINTVGIILMVAGVVGL
                     LLSLLFWSSFSPYRRRSTAAYPEERVVEERRIERDYP"
     gene            1768..2481
                     /locus_tag="VFO47_03985"
     CDS             1768..2481
                     /locus_tag="VFO47_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET8787888.1"
                     /translation="MAAIGDSITQAVDVCCFYGNWPGHSWATGYVPLDGIASHYERIR
                     ARNPAIRGHRWNNAVSGARMADAPGQARRTVGQGAEYVTILLGANDLCGWDGRLTPTA
                     TFRAQFAETLDILRTGLPGSRVFVASIPNLYQLWSVLRTDPVAQIVWQAAGICPSMLN
                     FFNSAADRQAVIEREREFNDVLRDVCATWSNCRFDGYLTYEYDFSRGMVSRLDYFHPS
                     LAGQATLAALTWGRSWWGS"
BASE COUNT          352 a         1005 c          924 g          403 t
ORIGIN      
        1 cgtcggtggc accgtggcca ctctgctggc caaccgcctc aagtcccttg aggcgatcgc
       61 cgccgcctcc gaggaggagc tgaacgacgt cggcggggtc ggccccacca tcgccgccag
      121 cgtggccgcc tggttcgccg acgagggcaa ccaggacctg gtccggcgcc tggtcgaggc
      181 cggcctcaac accaaggccg acggcggcgg cggggccgac ctgccccaga ccctggccgg
      241 gatgtccttc gtcctcaccg gcggcctgga cggcttcacc cgcgacgagg ccacccgggc
      301 catcgaggac cgcggcggcc gggtcgcctc cagcgtctcc aagaagaccg cctacgtcgt
      361 cgtcggtgcc gaccccggct ccaaggccgc ccgcgccgcc gagctcgggg tccccaccct
      421 cgacgaggcc gccttcgccg ccctcctcgc caaggaggag gtcgaggagg tggccgatgg
      481 gtgacgtctg gcacccgctc gacccggagg ggtcgcggcc ccagcagccg tcggggcggg
      541 accggggacc cgacccggag ccgatcgtgc ggacggtgcg ggtcgcgttc ctgttcgccg
      601 ggatggtggt ggcggtgatg gtcagcgccg gcctcaactc cctcggcggg ccgggggcgg
      661 tggcgacgcc ggtgggcggg gtcgccgtca ccgccgtggt ggccgtggcc gtgctggccg
      721 tcctgggtgg cgccgggctg gccctgcgcc cggccaggct gctggtggcc ggcaagcggg
      781 ccctcgagca tcccgacccg cgcctgcgcc ggccgcgggc cgagcgggcc agggcgatcg
      841 gcttccgggg cctggccatg ggctgggcca gccaggccct ggtcttcggc ctcctcccgg
      901 cgttcgccgg cctggtgctg gaactgctct acgaccgcca gtgggagctg ctggccttcg
      961 ccgggatcag cctgctcgcc gggttcgtct tccagctcca ggtggccggg gccgtgcgcc
     1021 tggccgtgga cgaccccgag ctgcgcgcca gctacggctc ccgctgatag ggtcccccgg
     1081 tctggatcgg gggtttccac ggggcccgac cgggtaacgc ggaccggaga catcgtgctt
     1141 ggtttgaagg aggagcaatg agtatcggag ccagcatctt cctgctggtc atcggagcca
     1201 tcctgacgtt cgcggtcaac gtcaccaccg agggcttcaa catcaacacc gtcggcatca
     1261 tcctgatggt cgccggcgtg gtcggcctgc tcctgtccct gctgttctgg agcagcttct
     1321 ccccctaccg gcgccgctcc acggccgcct accccgagga gcgcgtggtc gaggagcgcc
     1381 gcatcgagcg cgactacccc tgagccgggc gttcacgcac aagcacatat ctaggcagga
     1441 accctaaaac cggtcattct ctgaccagca tcccgttcca cgcgaccggg gaggtcagag
     1501 atgcccagaa cggcggacag gtatccggcg ccgcatactg cgtcgaggca tccgtccagc
     1561 gtgaaggctt gcgcgccgcc cccggcgacc aggcagccgc cgcaagcgcg gatacgtgtc
     1621 cggtgttctg gatcgttccg cgccctcggg gtcggggtcg ccctgctcgc ggcgaccctg
     1681 ggtcccgcca ccgcagcctc cgccgcaacc tcggcccctg ccgccgcccc cctggccgcg
     1741 gcggtggcgc cgccgctgcc ggactcgatg gcggccatcg gggactcgat cacccaggcg
     1801 gtcgacgtct gctgcttcta cggcaactgg cccggccact cctgggccac cggatacgtg
     1861 ccgctggacg ggatcgccag ccactacgag cgcatccggg cccggaaccc ggccatccgc
     1921 ggccaccggt ggaacaacgc cgtctcgggc gcgcgcatgg ccgacgcccc cggccaggcc
     1981 cggcgcaccg tcggccaggg ggccgagtac gtcaccatcc tcctgggcgc caacgacctg
     2041 tgcggctggg acggccgcct cacccccacg gccaccttcc gggcccagtt cgccgagacc
     2101 ctcgacatcc tgcgcaccgg gctgcccggc agccgcgtgt tcgtggcctc gatcccgaac
     2161 ctgtaccagc tctggagcgt cctgcgcacc gacccggtcg cccagatcgt ctggcaggcg
     2221 gccggcatct gcccgtcgat gctgaacttc ttcaactcgg ccgccgaccg ccaggccgtg
     2281 atcgagcgcg agcgggaatt caacgacgtc ctccgcgacg tctgcgccac ctggtccaac
     2341 tgccgcttcg acggctacct gacctacgag tacgacttct cccgcggcat ggtctcgcgc
     2401 ctcgactact tccaccccag cctggccggc caggccaccc tggccgctct cacctgggga
     2461 cgatcctggt gggggagcta gcgaggttca gggattccta gtggttcgca cgacttgagt
     2521 ccgaccgggc ggacggttac tgtcccgcgc cgtaggacaa ttcgctctga tgcaaggttc
     2581 gtgcgagtga gcagctccaa ttcccgggag gccgatgaac caggtcgggt acgtgatcct
     2641 gttcgtgggg gacctggagc ggtcgaccgc gttctaccgg gacg
//
LOCUS       DASSWR010000235         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_461593,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000235 DASSWR010000000
VERSION     DASSWR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461593"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFO47_03990"
     CDS             complement(<1..223)
                     /locus_tag="VFO47_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787889.1"
                     /translation="MTTGGPERDEEERPLRRAPQLAVLLEGGDRKGLLHDMTAAILRH
                     DGDIVLVEIIERGARSVVYWELDGVDDPEG"
     gene            326..400
                     /locus_tag="VFO47_03995"
     tRNA            326..400
                     /locus_tag="VFO47_03995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:361..363,aa:Arg,seq:ccg)
     gene            538..939
                     /locus_tag="VFO47_04000"
     CDS             538..939
                     /locus_tag="VFO47_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8787890.1"
                     /translation="MSNARILVVDDDQVIQQLLKVNLELEGYAVEVASDGEEALALFD
                     RFHPDLVLLDIMMPKLDGWEVARRLAGTTGGPPPIVLLSARAQESDVQKGNDLGVAAY
                     VTKPFDPIQLLHLVAGIIAQQDRGAATPGVP"
     gene            936..>2565
                     /gene="argS"
                     /locus_tag="VFO47_04005"
     CDS             936..>2565
                     /gene="argS"
                     /locus_tag="VFO47_04005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET8787891.1"
                     /translation="MTAAVLAVQHDLAGLFRAALHRAVGAGALALDPEAIPEPSLERP
                     RLPEHGDWATNVAMVLAKAAKAPPRAVAEAMVAHLEPPDWVAGVEVAGPGFVNVRLDQ
                     RWFAGLVRRVLAEGRSFGAVDVGHGERVNVEFISANPTGPLHVGNARLAPTGDALANL
                     LAATGYKVEREYYLNDAGSQLDRLGASVEAAYLRLLGRPAETPADGYKGAYVAELAAE
                     LRSEQGDALADLEPAERLSRITEWSYRRMVEGIQATLARLGVTFDVWFSERTLHQSGA
                     IADTVDELRERGLVEDRDGAVWLRTTEFGDDKDRPLIRSSGAPTYLAADVVYYRDKRR
                     RGFDRLIFLWGADHHGYIPRMRAVVRAFGDPDDTAEFLIGQLVSLVRAGQPAKMSKRS
                     GDIVTVDDLIDEVGQGRLPLHDAALLARRADRVRRRGGHPPVARQPGLLRPVRARPHL
                     QRHPPGQRDRVQPPPGRGGRPRPAHPPDGGRSPAPPGRVRGAGPHGRPPARPPPPDPL
                     RRGPGRRLPPLLRRVPGPDRRPCPLQRPLVARAQH"
BASE COUNT          346 a          970 c          878 g          371 t
ORIGIN      
        1 gcccctccgg gtcgtcgacg ccgtccagct cccagtagac gacgctgcgg gccccgcgct
       61 cgatgatctc caccagcacg atgtcgccgt cgtggcgcag gatcgccgcc gtcatgtcgt
      121 gcagcagccc cttgcggtcc ccgccctcga gcagcacggc cagctgcggc gcgcgccgca
      181 gcggtcgctc ctcctcgtcc cgttcggggc cgcctgtcgt cacggacgag aggctaccag
      241 ccgggatggt cgggcccggg cgggccgcgg gtggcgcgtc cggccgtcta caggcccgtt
      301 ccagttctgt atcatcgggc cgcatgcccg tgtagctcag catggataga gcgtctgcct
      361 ccggagcaga aggtcgcccg ttcgagtcgg gccacgggta ccttcgacgg ggccgaaccg
      421 cttggccgcg ccgaatcggg attgatgcga acctgcaggt caaagggcct gtgctatgct
      481 ccgcggaccc gcgtcgcacg atcttcccgg actcctttct gatcaggtct ttcccgcatg
      541 tccaacgcgc gcatcttggt cgttgacgac gatcaggtca tccagcagct gctcaaggtc
      601 aatctggaac tggaggggta cgcggtcgag gtcgcctcgg acggcgagga ggcgctcgcg
      661 ctgttcgacc ggttccatcc ggacctggtg ctgctcgata tcatgatgcc caagctcgac
      721 ggctgggagg tcgcgcgccg gcttgccggg accaccggcg ggccgccgcc gatcgtgctg
      781 ctgtcggccc gagcgcagga gtccgacgtc cagaagggca acgacctggg cgtcgccgcc
      841 tacgtgacca agccgttcga tccgatccag ctcctgcacc tggtggcggg gatcatcgcc
      901 cagcaggacc gcggggccgc cacccctgga gtgccatgac cgccgccgta ctcgccgtcc
      961 agcacgacct ggctgggctc ttccgggccg ccctccaccg ggcggtcggc gccggggcgc
     1021 tcgccctcga ccccgaggcc attcccgagc cctcactgga gcggccgcgg ctgcccgagc
     1081 atggcgactg ggccaccaac gtggccatgg tgctggccaa ggcggccaag gcgccgcccc
     1141 gggcggtcgc cgaggccatg gtcgcccacc tggagccgcc cgactgggtg gccggggtcg
     1201 aggtggccgg tcccgggttc gtcaacgtcc ggctcgacca gcgctggttc gccgggctgg
     1261 tccggcgggt cctggccgag ggcaggtcgt tcggcgccgt cgacgtgggc cacggggagc
     1321 gggtcaacgt cgagttcatc tcggccaacc cgaccgggcc gctgcacgtc ggcaacgccc
     1381 gcctggcccc gaccggcgac gccctggcca acctgctcgc cgccaccggc tacaaggtcg
     1441 agcgcgagta ctacctcaac gacgccggca gccagctcga ccggctcggg gccagcgtcg
     1501 aggcggccta cctgcgcctg ctgggccggc cggccgagac cccggccgac gggtacaagg
     1561 gcgcctacgt cgccgagctg gccgcggagc tccgctcgga gcagggcgac gccctggccg
     1621 acctggagcc ggccgagcgc ctctcccgca tcaccgagtg gtcctaccgg cggatggtgg
     1681 aggggatcca ggcgacgctg gcccgcctcg gggtcacgtt cgacgtctgg ttctccgagc
     1741 gcaccctcca ccagagcggg gccatcgccg acaccgtgga cgagctgcgc gagcggggcc
     1801 tggtcgagga ccgcgacggc gccgtgtggc tgcgcacgac cgagttcggg gacgacaagg
     1861 accggccgct gatccgctcc agcggggccc ccacctacct ggcggccgac gtcgtctact
     1921 accgggacaa gcgccggcgc ggcttcgacc ggctgatctt cctgtggggc gccgaccacc
     1981 acggctacat cccgcggatg cgggcggtgg tgcgcgcctt cggcgacccc gacgacaccg
     2041 ccgagttcct catcggccag ctggtgagcc tggtccgggc gggccagccg gccaagatga
     2101 gcaagcggtc cggcgacatc gtcaccgtcg acgacctgat cgacgaggtc ggtcaaggac
     2161 gccttccgct acacgatgct gcgcttctcg ctcgacgcgc cgatcgagtt cgacgtcgag
     2221 gcggtcaccc gccagtcgct cgacaacccg gtctactacg tccagtacgc gcacgcccgc
     2281 atctccagcg tcatccgcca gggcagcgag atcgggttca gccccctccc ggtcgaggag
     2341 gccgacctcg gcctgctcac ccacccgatg gaggccgctc tcctgcgccg cctggccgcg
     2401 tacgaggagc tggtcctcac ggccgcccgc cagcgcgccc cccaccgcct gacccgctac
     2461 gccgaggacc tggccgccgc cttccaccgc ttctacggcg agtgccgggt cctgaccgac
     2521 gaccatgccc tctccagcgc ccgctggtgg ctcgtgcaca gcatc
//
LOCUS       DASSWR010000236         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_263626,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000236 DASSWR010000000
VERSION     DASSWR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263626"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..447)
                     /locus_tag="VFO47_04010"
     CDS             complement(85..447)
                     /locus_tag="VFO47_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787892.1"
                     /translation="MAGEVAFFELGVEDVGRGRAFYEGLFGWRFETGPSGEGLTIGTG
                     GVPGGMHGGDKGAAPYLFFRVDDMEAALARVRELGGVVEEMGVEGDEDSVARWGRFKL
                     CRDDQGSPFGLHQPPSGS"
     gene            complement(516..1307)
                     /gene="map"
                     /locus_tag="VFO47_04015"
     CDS             complement(516..1307)
                     /gene="map"
                     /locus_tag="VFO47_04015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET8787893.1"
                     /translation="MITRKSPEEIDRMRRAGRLVGHTLSRVVEAVRPGVTLLELDALA
                     ERVIRDGGGTPSFLGYHGFPATLCLSPNDRIVHGIPNGYVLQEGDILSVDCGAIVDGW
                     HGDAAVTVPVGQVDDAARRLIETTERAMWAGIARVRAGNRLSDIGHAVERAAAPGYGV
                     VREYVGHGIGTRMHEEPQVPNYGRPGRGLRLEVGLALAIEPMVNEGGPSSRVLDDGWT
                     VVTSDGSRSAHFEHTVAITPQGPEVLTLLDNIRQEDWLPTAAQRR"
     gene            complement(1304..1882)
                     /locus_tag="VFO47_04020"
     CDS             complement(1304..1882)
                     /locus_tag="VFO47_04020"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET8787894.1"
                     /translation="MRLVVLGPPGAGKGTQAVKLAEHFTCADVATGDIFRANVAEGTE
                     LGRAAQEFMDRGDLVPDDVVIAMVMERLASDDCASGFVLDGFPRTVNQAEALDRRLAE
                     LGTPLEAALSFDVTEEELLRRLAGRAAAQHRADDAEQTIRHRLEVFAIKTRPLIDYYA
                     HRGLLVNVDAIGPIEVVTKRILDDLEGNRRPG"
     gene            complement(1879..>2195)
                     /locus_tag="VFO47_04025"
     CDS             complement(1879..>2195)
                     /locus_tag="VFO47_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET8787895.1"
                     /translation="YNSIAFNPVDTADNMKKYGGFIPGIRPGRQTAEHLNYILTRITL
                     PGAVFLGAVAILPFIALAAGNVQSFPFGGTSILITVGVGLETMKQIESQLLMRNYEGF
                     LR"
BASE COUNT          300 a          808 c          786 g          301 t
ORIGIN      
        1 cgccctccag ggcgagctgc tggccgtggt cgaccagacc ctgcagccga ccagcgcctc
       61 gctgtggctg cgggcgccgg ccggtcagga accgctcggc ggctgatgca gcccgaacgg
      121 cgacccctgg tcgtcgcggc agagcttgaa ccggccccac cgggcgaccg agtcctcgtc
      181 cccctcgacg cccatctcct cgaccacgcc gccgagctcc cgcacccggg ccagggccgc
      241 ctccatgtcg tcgacccgga agaacaggta cggcgccgcc cccttgtccc caccatgcat
      301 cccgccgggc acgccccccg tcccgatcgt gagcccctcc ccggacggcc ccgtctcgaa
      361 ccgccacccg aacagcccct cgtagaacgc ccggccacgc cccacgtcct ccacgcccag
      421 ctcgaagaac gccacctcac cggccaccgc tgtctcctct cccccgtcgg cgaacccgcc
      481 aacgctaccg ttcgcggccg acagccggaa ccggcctacc ggcgctgagc cgcggtgggg
      541 agccagtctt cctggcggat gttgtcgagg agggtgagga cctcggggcc ctggggggtg
      601 atggcgacgg tatgctcgaa gtgggcggag cgggaaccgt cgctggtgac gacggtccag
      661 ccgtcgtcga ggacgcggga ggaggggccg ccctcgttga ccatcggctc gatggccagg
      721 gcgaggccga cctcgaggcg gaggccgcgg ccggggcggc cgtagttggg gacctggggc
      781 tcctcgtgca tgcgggtgcc gatgccgtgg ccgacgtact cgcggaccac gccgtagccg
      841 ggggcggcgg cccgctcgac cgcgtggccg atgtcggaca ggcggttgcc ggcgcggacc
      901 cgggcgatgc cggcccacat ggcccgctcg gtggtctcga tcagccgccg ggccgcgtcg
      961 tcgacctggc cgacggggac cgtcacggcg gcgtcgccgt gccagccgtc gacgatggcg
     1021 ccgcagtcga ccgagaggat gtccccctcc tggagcacgt agccgttggg gatcccgtgg
     1081 acgatcctgt cgttgggcga caggcacagg gtggccggga acccgtggta gccgaggaac
     1141 gacggggtcc cgccgccgtc gcggatgacc cgctcggcca gcgcgtccag ctccagcagg
     1201 gtgaccccgg gccggacggc ctcgaccacc cgcgacaggg tgtgcccgac cagccgtccg
     1261 gcccgccgca tgcggtcgat ctcctccggg gacttgcggg tgatcacccc ggcctccggt
     1321 tcccctcgag gtcgtcgagg atccgcttgg tcacgacctc gatcggcccg atggcgtcga
     1381 cgttgacgag caggccgcgg tgggcgtagt agtcgatcag gggccgggtc ttgatcgcga
     1441 acacctcaag gcggtgccgg atcgtctgct cggcgtcgtc ggcccggtgc tgggccgccg
     1501 cccgcccggc cagccgccgc agcagctcct cctcggtgac gtcgaagctg agggccgcct
     1561 ccagcggggt gcccagctcg gccaggcgcc ggtccagggc ctcggcctgg ttgacggtgc
     1621 gggggaagcc gtccagcacg aaccccgagg cgcagtcgtc ggaggccagg cgctccatga
     1681 ccatggcgat gaccacgtcg tcgggcacca ggtcgccccg gtccatgaac tcctgggcgg
     1741 cccggcccag ctcggtcccc tcggccacgt tggcccggaa gatgtccccg gtggccacgt
     1801 cggcgcaggt gaagtgctcc gccagcttga ccgcctgggt tcccttgccc gcccccggcg
     1861 ggccgaggac caccagcctc aacggagaaa gccctcgtag ttgcgcatca ggagctggga
     1921 ctcgatctgc ttcatggtct ccagcccaac cccgaccgtg atcaggatcg acgtgccccc
     1981 gaacgggaac gactgcacgt tgccggcggc cagggcgatg aagggcagga tggccacggc
     2041 gccgaggaac accgccccgg gcagggtgat ccgggtcagg atgtagttca ggtgctcggc
     2101 ggtctggcgg ccgggccgga tgccggggat gaagccgccg tacttcttca tgttgtcggc
     2161 ggtgtcgacc gggttgaagg cgatcgagtt gtaga
//
LOCUS       DASSWR010000237         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_687722,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000237 DASSWR010000000
VERSION     DASSWR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687722"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFO47_04030"
     CDS             complement(<1..198)
                     /locus_tag="VFO47_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787896.1"
                     /translation="MAVTTYPGHREADVVLRDGSTVHVRPARLADASEIERLLKGLSD
                     RSRWLRFFSGYPNLVKAVQWAT"
     gene            complement(198..791)
                     /locus_tag="VFO47_04035"
     CDS             complement(198..791)
                     /locus_tag="VFO47_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8787897.1"
                     /translation="MRVRDVMSSPVVTVPPDMPLKEVAKLLVTRGVSAVPVVDRGELA
                     GILSEADLIPLELAPDPRAHLAPLPAAPATSRIASEAMTRDVVTLPEEADVADAGRLM
                     LERRIKSIPVLRGRRVVGIVARRDLLEVLARSDEEITRDLEALLAAELGAPSRYRVTV
                     RDGVVDLTGPPDPVDRQLATLLARGVPGVLEVRLDEE"
     gene            947..1639
                     /locus_tag="VFO47_04040"
     CDS             947..1639
                     /locus_tag="VFO47_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8787898.1"
                     /translation="MHSLVKDLMTTPVVTVEPSAPFKEIVARLAEHRVSAVPVVDDGR
                     RVLGVVSEADLLLKEEFPEPEQDLPQWWTKRARLDRAKAAASVARDLMTVAVVAVSPD
                     ATVAEAARRMHTAKVKRLPVVDRTGRLVGIVSRSDLLKVFDRSDQDIRREIIEDVIVG
                     EFTMAPSRFFLHVTDGVVVLQGRVEGRSLLPYLVRAVQGVEGVVRVENRLTYDIDDRD
                     AGRVMAFPWIRT"
     gene            complement(1686..>2140)
                     /locus_tag="VFO47_04045"
     CDS             complement(1686..>2140)
                     /locus_tag="VFO47_04045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8787899.1"
                     /translation="YLPGEPGPPIHLRQRADGRVLVGERSQETVASDPGRRRARALLA
                     QAARFFPALAGAGVDKWWVAYRAMPSDRLPIVGPLPWLDGLYVAVSHSGVTVAPVLGR
                     LVADEVATQAADGLLAPFRPGRFAERATRVLLEVESLFREPTRQDRST"
BASE COUNT          301 a          773 c          785 g          281 t
ORIGIN      
        1 ggtcgcccac tggacggcct tgaccaggtt cggatagccg gagaagaacc ggagccagcg
       61 gctgcggtcg gacagcccct tgagcagccg ctcgatctcg gacgcgtcgg cgagcctggc
      121 cggccgcacg tggaccgtcg agccgtcccg caggacgacg tcggcctccc ggtgaccggg
      181 ataggtggtc acggccatca ctcctcgtcc aaccggacct cgaggactcc gggcaccccg
      241 cgggccagta gcgtggccag ctggcgatcg accgggtcgg gagggccggt gaggtcgacg
      301 accccgtccc ggaccgtcac ccggtagcgg cttggcgcgc ccagttcggc cgccagcagc
      361 gcctccaggt cacgggtgat ctcctcgtcg ctgcgggcca gcacctccag caggtcccgc
      421 cgggccacga tgccaacgac ccggcggccc cggaggaccg ggatcgactt gatgcgccgt
      481 tccagcatga gccggccggc gtcggcgaca tcggcctcct cgggcagcgt cacgacgtcc
      541 ctggtcatcg cctcggacgc gatccgggag gtcgccggtg cggcggggag tggcgcgaga
      601 tgggcccgcg ggtcgggagc cagctccagt gggatcagat cggcctcgga caggatgccg
      661 gccagctcgc cgcggtccac caccggcacg gcgctgacgc cgcgcgtgac caggagcttg
      721 gcgacctctt tgagcggcat gtccggcggc accgtgacca ccgggctgga catgacgtcc
      781 ctgactcgca cgccgacctc cttgcatcgg ttgccaccag tctggcctgg ggtccgaagt
      841 gctcccaggg ccaggcagcc cagccggcgg gggaccatcg cccctacagc gcgggcaggc
      901 cgggcggttg cattgctcta gggggcctga accaagggag gaccccatgc acagcctggt
      961 caaggacctg atgacgacgc cggtggtgac ggtggagccc agcgccccgt tcaaagagat
     1021 cgtggcccgc ctggccgagc accgggtcag cgcggtcccg gtcgtggacg atggtcgccg
     1081 ggtgctgggc gtcgtctccg aagccgatct gctcctcaag gaggagttcc ccgagcccga
     1141 gcaggacctc ccgcagtggt ggaccaagcg ggcccggctg gaccgggcca aggccgccgc
     1201 ctcggtggcc cgcgacctca tgaccgtggc cgtggtggcc gtctcgcccg acgcgacggt
     1261 ggccgaggcg gcccggcgga tgcacaccgc caaggtcaag cggctgcccg tggtcgaccg
     1321 caccggtcgg ctggtcggga tcgtgagccg cagtgacctg ctgaaggtgt tcgaccgctc
     1381 cgaccaggac atccgccgcg agatcatcga ggacgtgatc gtgggcgagt tcacgatggc
     1441 gccgagccgg ttcttcctcc atgtcaccga cggggtggtc gtgctccagg gccgggtcga
     1501 ggggcgcagc ctcctcccct acctcgtccg ggccgtccag ggggtcgagg gcgtggtccg
     1561 ggtcgagaac cgactgacct acgacatcga cgaccgcgac gccggccggg tgatggcctt
     1621 cccctggatt cgtacctgac cccgacgcct cagtaccgga acatgggctt ggcggcgctc
     1681 gtcagtcagg tggagcggtc ctggcgggtc ggctcgcgga acagcgactc gacctcgagc
     1741 agcaccctgg tggcgcgctc ggcgaagcgg cctgggcgga agggggccag caggccgtcg
     1801 gccgcctggg tggcgacctc gtccgcgacc aggcgcccca ggaccggggc cacggtgacc
     1861 ccgctgtggc tgacggccac atacagccca tccagccagg gcagcggccc cacgatcggc
     1921 agccggtccg acggcatcgc ccggtaggcc acccaccact tgtccacccc ggccccggcc
     1981 agggccggga agaaccgggc cgcctgggcc agcagggccc gggcccgccg gcggccgggg
     2041 tcgctggcca ctgtctcctg gctgcgctcc ccgaccagca cccgcccgtc ggcccgctgg
     2101 cgcaggtgga tcgggggccc gggttcgccg ggcaggtaga
//
LOCUS       DASSWR010000238         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_461654,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000238 DASSWR010000000
VERSION     DASSWR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461654"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFO47_04050"
     CDS             <1..216
                     /locus_tag="VFO47_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787900.1"
                     /translation="FTTLSVPVMTRLRLPERRVLDTLIDAGVARSRSDALAWCVRLVG
                     THEADWISELRTALVAVKRVRAQGPNP"
     gene            complement(213..1718)
                     /locus_tag="VFO47_04055"
     CDS             complement(213..1718)
                     /locus_tag="VFO47_04055"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET8787901.1"
                     /translation="MKVTLAKADAVSAKADLLAVPVFAGPELGPGAEEAVAALGAPVA
                     PLLEARGFTGKAGEAAALPTLGRLPATVLLLVGMGERAKVDAEVLRRAAATVVRQGRG
                     ARKAVTTLTQALPADPAGAVQAVTEGALLAAYRFDKYKHAQQAKTNGTGQPAELTALV
                     LHTGGGRGPGGLAGALAAGQARAAATNLARDLSNEPANALHPADLAAARKLLAGKGVT
                     VKVKDEKALAAEGFGGIIGVGQGAEHPPRLIELRYAPKGAAGEVVLVGKGITFDSGGL
                     SLKPADSMKTMKTDMSGAAAVLATMSVLADLDVKVGVTGYLASAENMPSGRATRPGDV
                     LTMKNGKTVEVLNTDAEGRLVMADALALGADAKPDAIIDVATLTGACAIALGNRYTGL
                     MANDEALAAELLDAAAEAGEPTWRLPLPPEYRKDIESDLADLKNVGDRYGGALFAGLF
                     LQEFVDGLPWAHLDIAGPARAESEDGYLSKGSTGVTVRTLLTWLERRGATR"
     gene            1775..2095
                     /locus_tag="VFO47_04060"
     CDS             1775..2095
                     /locus_tag="VFO47_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8787902.1"
                     /translation="MDRTRLADLIAFDERRMAKHRVFETDRTLTDLYCLEPGQEQLLH
                     VHEGSDKVLVVMAGRVLVRVDDDTAEAGQYEAVLAAAGSKHALKNLGPEPAVLLVFVG
                     PRLA"
     gene            complement(2107..>2564)
                     /locus_tag="VFO47_04065"
     CDS             complement(2107..>2564)
                     /locus_tag="VFO47_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787903.1"
                     /translation="ERAHGVAGTEVLYLADHAVPELQETGIPEHEKRTPAPASRSTAE
                     FPAPLRRCLEAARFEGLPRIRLVEDWDDPAIPSEPVPGQYRSPGDPWRRIAGGRLFYL
                     GGVPDRGDRLALAASSAGGLGFDYSGGLFVLERRAGAWRVTDQARYWVS"
BASE COUNT          310 a          939 c          977 g          338 t
ORIGIN      
        1 ttcaccaccc tgagcgtgcc ggtgatgacg cggctgcggc tgccggagcg gcgggtgctc
       61 gacaccctga tcgacgccgg ggtcgcccgc agccgcagcg acgccctggc ctggtgcgtg
      121 cggctggtcg gcacccacga ggccgactgg atcagcgagt tgcgcacggc cctggtcgcc
      181 gtcaagcgcg tccgcgccca gggccccaac ccctaacggg tggcgccgcg gcgctccagc
      241 caggtgagca gggtgcggac agtgacgccg gtggagccct tggagaggta gccgtcctcg
      301 gactcggcgc gggcggggcc ggcgatgtcg aggtgggccc aggggaggcc gtcgacgaac
      361 tcctggagga acaggccggc gaacagggcg ccgccatagc ggtcgccgac gttcttgagg
      421 tcggccaggt cgctctcgat gtccttgcgg tactcgggcg gcagggggag gcgccaggtc
      481 ggctcgccgg cctcggcggc ggcgtccagc agctcggcgg ccagagcctc gtcgttggcc
      541 atgagaccgg tgtagcggtt gccgagggcg atcgcgcagg cgccggtgag ggtggcgacg
      601 tcgatgatcg cgtcgggctt ggcgtcggcg ccaagggcga gggcgtcggc catgaccagg
      661 cggccctcgg cgtcggtgtt caggacctcg acggtcttgc cgttcttcat ggtgagcacg
      721 tcgccggggc gggtggcgcg gccggacggc atgttctcgg ccgaggccag gtagccggtg
      781 acaccgacct tgacgtccag gtcggccagg acgctcatgg tggccaggac cgccgccgcc
      841 cccgacatgt cggtcttcat ggtcttcatc gagtcggccg gcttgagcga cagcccgccc
      901 gagtcgaagg tgatgccctt gccgaccagg accacctcgc cggcggcgcc cttgggcgcg
      961 tagcgcagct cgatcaggcg cggcgggtgc tcggcgccct gcccgacccc gatgatgccg
     1021 ccgaagccct cggcggccag cgccttctcg tccttgacct tgacggtgac gcccttgccg
     1081 gccagcagct tgcgggcggc ggccaggtcg gccgggtgca gggcgttggc cggctcgttg
     1141 gacaggtccc tggccaggtt ggtggcggcc gcccgggcct gcccggcggc cagggcgccg
     1201 gccagcccgc ccggtccacg cccgccgccg gtgtgcagga ccagggcggt cagctcggcc
     1261 ggctgccccg tcccgttggt cttggcctgc tgggcgtgct tgtacttgtc gaagcggtag
     1321 gcggccagca gcgccccttc ggtcaccgcc tgcacggcgc cggccgggtc ggcgggcagc
     1381 gcctgggtca gggtggtcac cgccttgcgg gccccgcggc cctggcggac caccgtggcc
     1441 gcggcccggc gcagcacctc ggcgtcgacc ttggcccgct cccccatccc gaccagcagc
     1501 agcacggtgg ccggcagccg gccaagggtc ggtagggcgg ccgcctcgcc ggccttgccg
     1561 gtgaacccgc gcgcctccag cagcggcgcc accggcgccc ccagggcggc caccgcctcc
     1621 tcggcgcccg ggcccagctc cgggccggcg aacaccggca cggcgagcag atcggccttg
     1681 gcgctgaccg cgtcggcctt ggcgagcgtg accttcatgg accttcgtcc ctccgtcggt
     1741 gcaggtggcg gcaaggagct aggctagcag tcccatggac cgaaccagac tggccgacct
     1801 gatcgccttc gacgagcggc gcatggccaa gcaccgggtg ttcgagaccg accgcaccct
     1861 gaccgacctc tactgcctgg aaccggggca ggaacagctg ctccacgtcc acgagggctc
     1921 cgacaaggtc ctggtggtga tggccgggcg ggtgctcgtc cgggtcgacg acgacaccgc
     1981 cgaggccggc cagtacgagg ccgtgctcgc cgccgccggg tccaagcacg ccctcaagaa
     2041 cctcggcccc gaaccggccg tcctgctggt gttcgtcggc ccccgcctgg cctgagcgcc
     2101 ggccgctcag gacacccagt agcgagcctg gtcggtgacc cgccaggcac cggcgcggcg
     2161 ctccaggacg aacaggccgc cgctgtagtc gaagccgagg ccgccggccg agctggccgc
     2221 cagggcgagc cggtcgccgc ggtcgggcac cccgccgagg tagaagaggc ggccgccggc
     2281 gatccggcgc catgggtcgc ccggcgaccg gtactgcccg ggaacgggct cgctcgggat
     2341 cgccgggtcg tcccagtcct cgaccagccg gatccggggc aggccctcga accgggccgc
     2401 ctccaggcag cgccgcagcg gcgcggggaa ctcggcggtc gagcgcgacg ccggcgccgg
     2461 cgtccgcttc tcgtgctcgg ggatgccggt ctcctggagc tcggggacgg cgtggtcggc
     2521 caggtacagg acctcggtcc cggcgacgcc gtgggcgcgc tcgc
//
LOCUS       DASSWR010000239         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1224953, whole genome shotgun sequence.
ACCESSION   DASSWR010000239 DASSWR010000000
VERSION     DASSWR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224953"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1731)
                     /locus_tag="VFO47_04070"
     CDS             complement(247..1731)
                     /locus_tag="VFO47_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8787904.1"
                     /translation="MALADELAYVTATELAARIRRRELSPVEVVDAFIERIQARNQSL
                     NALVYLGFDNARQRAREAEQALVSGAELGVLHGVPTAIKDLFDFQPGWPATFGGIRAL
                     KDLTLDASCAFAERIQQAGAIVVGKTNSPIMGFRGTCDNYLFGPSRNPFDTAKNTGGS
                     SGGSAAAVADGLLPLAEGTDGGGSIRIPAAWCGVYGYKASFGRIPYVLRPNAFGGINP
                     FLFEGPITRTVEDAALALDALAGYDSRDPFSLDSDERFTPAVRRSISGWRIAYSPDFD
                     VFPVDKRVAEVVGGAVRAFEQAGAQVEEVRLGIERDQRELSDLWCRLIMPINVETFEN
                     FKRNGVDLLAEHREDFPPEYLRWIDEGYRMSALDLSRDQQTRSEIYDAIQRVLDDHHL
                     LVTPTLACLPVDNAEDGNTVGPSRINDVEVDPLIGWCLTYFVNFSGHPAASIPAGLAE
                     DRLPVGMQLIGRRYADADVLAASATFERLRPWQDTYQRCASRPL"
     gene            complement(1909..2934)
                     /locus_tag="VFO47_04075"
     CDS             complement(1909..2934)
                     /locus_tag="VFO47_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8787905.1"
                     /translation="MVDYEIFDLGDFRLQMGATLRDAKLAYKTYGTLDQDRSNAVVYP
                     TWYSGQHYDNEWLIGEGMALDPARYFIIVPNMLGNGLSSSPSNTPPPYDRARFPRVTV
                     DDNVTAQHRLVTERFGIQRLALVTGWSMGAGQTYQWAVSHPEMVPRILPFCGSAKTSE
                     HNVVFLEGVKVALTADGAFQEGWYETPPTKGLRAMARVYAGWGFSQAFYWQRLYHTEM
                     GYSSLEDFLVSFWEGFFLAKDANNLLAMLWTWQNGDIGKTPGFGGDFERALGAITARA
                     IVMPAEKDLYFPPEDNDYEVRHMPNAELRVIPGVWGHFAGGGLDADRTDTKFIDDALK
                     ELLAQEV"
BASE COUNT          509 a         1115 c         1090 g          538 t
ORIGIN      
        1 aggagcgtcg gcccagctac ttctggcgtt gggtcatatc ccggctgcaa cgtgctcgca
       61 ctacaccggc gaccccatcc ttaccatgga accaccggga accgctgtgc ggaccgccgt
      121 cttcgcaggt cgcgcccgac cgtcggggcc gaaggtatcg gttctccttc ggtgaagtta
      181 tgcgttcacc taccagctga tcgggattgg acttcgagcg ggctcttgag gatcggtagg
      241 ccgccattac agtggcctgc tcgcgcaccg ctggtaggtg tcctgccagg gcctgagccg
      301 ctcgaaggtg gcgctggcgg ccagcacgtc tgcgtcggcg tacctgcggc caatcagctg
      361 catcccgacc ggcaaccggt cctcggccaa gcccgcgggg atcgaggcgg ccgggtggcc
      421 ggagaagttg acgaagtagg tcaggcacca gccgatcagc ggatcgacct ccacatcgtt
      481 gatgcggctc gggccgacgg tgttgccgtc ctcggcgttg tccaccggca ggcaggccag
      541 ggtcggggtg accaggaggt ggtggtcgtc caggacccgt tggatggcgt cgtagatctc
      601 tgagcgggtc tgctggtcgc gggagaggtc cagcgcgctc atacggtagc cctcgtcgat
      661 ccaccgcagg tactcgggcg ggaagtcctc gcggtgctcg gcgagcaggt ccaccccgtt
      721 gcgcttgaag ttctcgaagg tctccacgtt gatcggcatg atgagccggc accacaggtc
      781 gctcagctcc cgctggtccc gctcgatccc cagccgcacc tcctcgacct gcgcaccggc
      841 ctgctcgaag gcgcgcacgg cgccgcccac gacctccgcg acgcgcttgt cgaccgggaa
      901 gacgtcgaag tcggggctgt aggcgatccg ccagccgctg atggaccgcc gcaccgccgg
      961 cgtgaagcgc tcgtcggagt cgaggctgaa tgggtccctg gagtcgtagc cggccaaggc
     1021 gtccaaggcc agcgcggcgt cctcgacggt cctggtgatc ggtccctcga acaggaacgg
     1081 gttgatgccg ccgaaggcgt tgggccggag gacgtagggg atgcgaccga aggaggcctt
     1141 gtagccgtac acgccgcacc aggcggcggg gatgcggatc gagcctcccc cgtcggtccc
     1201 ctcggccagg ggcaggagcc cgtcggcgac cgcggcggcg ctgcctcctg aggagccgcc
     1261 ggtgttcttg gcggtgtcga aggggttgcg gctggggccg aacaggtagt tgtcgcaggt
     1321 gccccggaac cccatgatcg ggctgttggt cttgccaacc acgatcgcgc cggcttgttg
     1381 gatccgctcg gcgaacgcgc acgacgcgtc gagggtgagg tccttgaggg cgcggatgcc
     1441 gccgaaggtc gccggccagc ctggctggaa gtcgaacagg tccttgatcg cagtgggcac
     1501 cccgtgcagc accccaagct ccgctccgga caccaacgcc tgctcggcct cccgggcccg
     1561 ttggcgggcg ttatcaaagc cgaggtagac cagggcattc aggctctggt ttctggcctg
     1621 gatgcgctcg atgaaggcat ccacgacctc aaccggggac agctcgcgcc gccggatccg
     1681 ggccgccagt tccgtcgccg tcacgtaggc aagctcatct gccaacgcca tcctggccca
     1741 cctcctccaa atggccgcct tctgtgcggc ggccgaccgt catcgcctgg tggcggcagg
     1801 ggtttccgtc ctgaccacac ggcctggtcc tcaagccaag gaccagaccc cgcgccgcgt
     1861 ggcccatctg gccgctgctt gcctccggtt cctcacagca ccggcacgtc atacctcctg
     1921 ggcgaggagc tccttgagcg cgtcgtcgat gaacttggtg tcggtccggt cggcgtcgag
     1981 cccaccgccg gcgaagtgcc cccagacgcc cgggatcacc cgcagctccg cgttgggcat
     2041 gtgcctgacc tcgtagtcat tgtcctcggg cgggaagtag aggtccttct ccgccggcat
     2101 cacgatcgcc ctggccgtga tcgcgccgag cgctcgctcg aagtcgccgc cgaagcccgg
     2161 cgtcttgccg atgtccccgt tctgccaggt ccacagcatg gccagcaggt tgttggcgtc
     2221 cttggccagg aagaagccct cccagaagga caccaggaag tcctcaagcg aggagtagcc
     2281 catctcggtg tggtacagcc gctgccagta gaaggcctgc gagaaccccc agccggcata
     2341 cacccgcgcc atggcgcgca ggcccttggt cggtggcgtc tcataccagc cctcctggaa
     2401 cgccccgtcg gccgtgagtg ccaccttgac cccctccagg aagacgacgt tgtgctcact
     2461 ggtcttggcc gagccacaga acggcaggat cctggggacc atctcgggat ggctgaccgc
     2521 ccactggtag gtctgtcccg cgcccattga ccagccagtc accagcgcca gccgctggat
     2581 gccgaaccgc tcggtgacca gccggtgctg ggcggtcacg ttgtcgtcca cggtcacccg
     2641 cgggaacctg gcccggtcgt aggggggtgg ggtgttgctc ggcgaggacg acagcccgtt
     2701 gccaagcatg ttcggcacga tgatgaagta cctggcagga tcgagcgcca tcccctcccc
     2761 gatcagccac tcgttgtcgt agtgctggcc ggagtaccag gtggggtaga cgacggcgtt
     2821 gctcctgtcc tggtcaaggg tcccataggt cttgtaggcg agcttggcat cccgcagggt
     2881 cgcccccatc tggagccgga agtcgcctag gtcgaagatc tcgtagtcca ccatcgctgc
     2941 ctcctcgcaa gctccgcggc cgcctcgccg gcaggacgcc ggcaccacca cgacggctcg
     3001 cggcgagtcg gacacaatct tcacgccgga caacacccga taccaggggg aactcgcaca
     3061 ccatctcgct ggccgaggcc aagcacgtct accagaaggc gccgtcatcg ccatgctccc
     3121 tgtgacctgc tgagacagtc accgccccgc gaccggagcg ctccaggacg gctccatagc
     3181 cgagggaggg ccttgccgca tggagggagg agacgcaggt gcgcttcagt cgcggcggcg
     3241 cagcggatgc tc
//
LOCUS       DASSWR010000240         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_235552,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000240 DASSWR010000000
VERSION     DASSWR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235552"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1349
                     /locus_tag="VFO47_04080"
     CDS             33..1349
                     /locus_tag="VFO47_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HET8787906.1"
                     /translation="MSADERAGLQARRLDDLVGRLAERSPLYRERLGGAGLGPGARVG
                     LDGLADLPFTTKQDLWDGYPWGLLAVPRSQVLRVHGSSGTGGRPTLVAYSRADLAIWA
                     EVCARALGCAGAGPGTVVHNAYGYGLFTGGLGMHAGAELMGCTLVPVSGGQTQRQVTL
                     LRDLRPEVLCCTPSYAARLGEALGEAGVGRADVSLRAGIFGAEPWSEAMRGQLEALLP
                     LKALDIYGLSEIIGPGVACECVEAQAGLHVNEDHFLVEVVDPDSGRPLGPGERGELVF
                     TTLTKEAMPLLRYRTGDIAALDPSPCACGRTLARMSKVTGRKDDMLVVRGVNVYPSEV
                     EAVLVAERAVGPQYLLVVDRTGTMPRLVVACELAAGWAICEETEAAERTRVAGEAQAA
                     LAERLGLTTEVRVLPAGTIPRIEMGKAQRVVERTPAHDPLAGLDGG"
     gene            1349..1699
                     /locus_tag="VFO47_04085"
     CDS             1349..1699
                     /locus_tag="VFO47_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787907.1"
                     /translation="MAIERTALFVGLAWLILNRYEATLVGYQLGVIPEVEWAVWTARL
                     EQDLQLPFIRDVWDQDVSNFDYTRGFKELVTRLLSPADGPGSGPWPVDGRGAPSGAYG
                     RLSRPCTPSSSPTT"
     gene            1669..>2119
                     /locus_tag="VFO47_04090"
     CDS             1669..>2119
                     /locus_tag="VFO47_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET8787908.1"
                     /translation="MHPKFLTHDLNPAQREAVETTVGPLCILAGAGTGKTRVISRRVA
                     YAIATGAVHPGHVLVVTFTDKAANEMRERLAALGFPGVQAQTFHAAAFRQLRYFWPRL
                     SEGRLPEVLDSKAPLLAPIQRSLPGGYKFTAVKDLADELEWAKARRLD"
BASE COUNT          262 a          795 c          781 g          281 t
ORIGIN      
        1 cggacaccat ctgggaccga ggggccgagc ggatgtcggc cgacgagcgg gcggggctgc
       61 aggccaggcg cctggacgac ctggtcgggc ggctggccga gcggagcccc ctgtaccggg
      121 agcggctggg cggggccggg ctcgggcccg gcgcccgggt cggcctggac gggctggccg
      181 acctgccgtt caccaccaag caggacctgt gggacggcta cccctggggc ctgctggccg
      241 tcccccgctc ccaggtcctc cgcgtccacg gctcctcggg caccggcggc cgccccaccc
      301 tggtcgccta ctcccgcgcc gacctcgcca tctgggccga ggtctgcgcc cgcgcccttg
      361 gctgcgccgg cgccggccct gggaccgtgg tgcacaacgc ctacggatac ggcctgttca
      421 ccggtggcct gggcatgcac gccggggccg agctgatggg ctgcacgctg gtgccggtct
      481 ccggcggcca gacccagcgc caggtgacgc tgctgcgcga cctgcggccc gaggtcctct
      541 gctgcacccc ctcctacgcg gcccggctgg gggaggcgct gggcgaggcg ggcgtgggcc
      601 gcgccgacgt gtcgctgcgg gccgggatct tcggggccga gccctggagc gaggccatgc
      661 gcggccagct cgaggcgctg ctgccgctga aggccctcga catctacggc ctctcggaga
      721 tcatcggccc cggcgtggcc tgcgagtgcg tcgaggccca ggccggcctc cacgtcaacg
      781 aggaccactt cctggtcgag gtcgtcgacc cggactcggg gcgcccgctg gggccggggg
      841 agcggggcga gctggtcttc accaccctga ccaaggaggc catgccgctg ctgcgctacc
      901 gcaccggcga catcgccgcc ctcgacccgt ccccctgcgc gtgcggccgg acgctggccc
      961 ggatgtccaa ggtgaccggg cgcaaggacg acatgctggt cgtccggggg gtcaacgtct
     1021 atcccagcga ggtcgaggcg gtgctggtcg ccgagcgggc cgtcggcccc cagtacctgc
     1081 tggtggtgga ccgcaccggg accatgccga ggctggtggt ggcgtgcgag ctggccgccg
     1141 gctgggcgat ctgcgaggag accgaggcgg ccgagcggac ccgggtggcc ggcgaggcgc
     1201 aggcggcgct ggccgagcgc ctcgggctca ccaccgaggt cagggtcctg cccgccggca
     1261 ccatcccccg gatcgagatg gggaaggccc agcgggtcgt cgagcggacc ccggcccacg
     1321 acccgctggc cggcctcgac ggtggctgat ggccatcgaa cgcaccgccc tgttcgtcgg
     1381 cctggcctgg ctgatcctca accgctacga ggccaccctg gtcggctacc agctcggggt
     1441 catcccggag gtggaatggg ccgtctggac ggcccgcctg gagcaggacc tccagctccc
     1501 gttcatccgc gacgtctggg accaggacgt ctcgaacttc gactacacca ggggcttcaa
     1561 ggagctagtc acccggctcc tctcgccggc cgacggcccc ggttccggcc cgtggcctgt
     1621 ggacggccgg ggagcaccgt cgggggcgta tggcagactc tcccgaccgt gcaccccaag
     1681 ttcctcaccc acgacctgaa cccggcgcaa cgggaggcgg tcgagaccac cgtgggcccg
     1741 ttgtgcatcc tggccggggc cgggaccggc aagacccggg tgatctcgcg gcgggtcgcc
     1801 tacgcgatcg ccaccggggc cgtccatccg gggcacgtgc tggtggtcac cttcaccgac
     1861 aaggcggcca acgagatgcg cgagcggctg gccgccctcg ggttccccgg ggtccaggcc
     1921 cagaccttcc atgccgccgc cttccgccag ctccgctact tctggccccg gctgtccgag
     1981 ggccggctgc ccgaggtgct cgactccaag gccccgctgc tcgccccgat ccagcgcagc
     2041 ctgcccgggg gatacaagtt caccgccgtc aaggacctgg ccgacgagct ggagtgggcc
     2101 aaggcccgcc gcctggacc
//
LOCUS       DASSWR010000241         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1393080, whole genome shotgun sequence.
ACCESSION   DASSWR010000241 DASSWR010000000
VERSION     DASSWR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393080"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFO47_04095"
     CDS             complement(<1..293)
                     /locus_tag="VFO47_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET8787909.1"
                     /translation="MRLVVLGPPGAGKGTQAVKLAEHFTCADVATGDIFRANVAEGTE
                     LGRAAQEYMDRGDLVPDDVVIAMVMERLASDDCASGFVLDGFPRTVNQAEALDR"
     gene            complement(290..1573)
                     /gene="secY"
                     /locus_tag="VFO47_04100"
     CDS             complement(290..1573)
                     /gene="secY"
                     /locus_tag="VFO47_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET8787910.1"
                     /translation="MLSAFRNAFKIPELRGKILFTVAIIAVYRLGSHLPVPGVDFDAV
                     QNYLENPAQDPSGAFTLINLFSGGALTQFAIFALGIMPYITSSIIMQLLGVVIPRLEQ
                     LQKEGEAGRKKIQQYTRYVTVFLALLQSASIVQLAPNQGILPIDIFPGLTPGRFALAV
                     MTLTAGTALIMWLGELITQRGVGNGMSILIFAAIVSTLPSEGANILRVNKAFIFGLVC
                     FLGLLIVVAVIFVEQAQRRIPVQYAKRMVGRRMYGGQSTYIPLKVNQAGVIPIIFASS
                     LLYIPVLLASIVNKEWLTDFVERFAQTGSHPLYIITYFLLIIGFAYFYNSIAFNPVDT
                     ADNMKKYGGFIPGIRPGRQTAEHLNYILTRITLPGAVFLGAVAILPFIALAAGNVQSF
                     PFGGTSILITVGVGLETMKQIESQLLMRNYEGFLR"
     gene            complement(1596..2033)
                     /gene="rplO"
                     /locus_tag="VFO47_04105"
     CDS             complement(1596..2033)
                     /gene="rplO"
                     /locus_tag="VFO47_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012751036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET8787911.1"
                     /translation="MKLHHLRPAPGSTKDRVRKGRGDASGKGKTAGRGTKGSKARSQI
                     RIGFEGGQMPLQRRVPKLRGFTNPNKVHWSVVNLDRLQAAFPRGGGVDPDGLVAAGLA
                     RKGTPVKVLGHGDVSVAFEVRAHAFSATAASKITAAGGTVEVV"
     gene            complement(2030..2218)
                     /gene="rpmD"
                     /locus_tag="VFO47_04110"
     CDS             complement(2030..2218)
                     /gene="rpmD"
                     /locus_tag="VFO47_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET8787912.1"
                     /translation="MSTRLKVTQKRSLIGRPRPQRDTVRSLGLKRIGDTVVKEDRPEI
                     RGMIFKVSHLVEVEEVQE"
     gene            complement(2215..>2670)
                     /gene="rpsE"
                     /locus_tag="VFO47_04115"
     CDS             complement(2215..>2670)
                     /gene="rpsE"
                     /locus_tag="VFO47_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET8787913.1"
                     /translation="GDGNGKVGVGYGKAKEVPSAIQKGVETAKKNFFPVPMIGSTIPH
                     EVLGAEGAGRVLLKPASPGTGVIAGGPVRAVLEAAGVSDVLSKSLGSSNPINIVHATV
                     AGLKQLRRPQEVADRRGLELAEMSPVTVARQKAAAAVGAGAGKAKEQSA"
BASE COUNT          405 a          897 c          948 g          420 t
ORIGIN      
        1 cggtccaggg cctcggcctg gttgacggtg cgggggaagc cgtccagcac gaaccccgag
       61 gcgcagtcgt cggaggccag gcgctccatg accatggcga tgaccacgtc gtcgggcacc
      121 aggtcacccc ggtccatgta ctcctgggcg gcccggccca gctcggtccc ctcggccacg
      181 ttcgcccgga agatgtcccc ggtggccacg tcggcgcagg tgaagtgctc cgccagcttg
      241 accgcctgcg ttcccttgcc cgccccgggc gggccgagga ccaccagcct caacggagaa
      301 agccctcgta gttccgcatc aggagctggg actcgatctg cttcatggtc tccagcccga
      361 ccccgaccgt gatcaggatg gacgtgcccc cgaacgggaa cgactgcacg ttgccggcgg
      421 ccagggcgat gaagggcagg atggccacgg cgccgaggaa caccgccccg ggcagggtga
      481 tccgggtcag gatgtagttc aggtgctcgg ccgtctgccg gccgggccgg atcccgggga
      541 tgaagccgcc gtacttcttc atgttgtcgg cggtgtcgac cgggttgaag gcgatcgagt
      601 tgtagaagta ggcgaacccg atgatcagca ggaagtaggt gatgatgtag agcgggtggc
      661 tgcccgtctg ggcgaaccgc tccacgaagt cggtcagcca ctccttgttc acgatcgagg
      721 ccagcagcac cgggatgtac agcaggctgg aggcgaagat gatcgggatg acgccggcct
      781 ggttgacctt cagggggatg taggtcgact ggccgccgta catccgccgg cccaccatcc
      841 gcttggcgta ctgcaccggg atccggcgct gggcctgctc gacgaagatc acggccacca
      901 cgatcagcag gccgaggaag cagaccagcc cgaagatgaa ggccttgttc acccgcagga
      961 tgttggcgcc ctcgctgggc agggtggaca cgatcgccgc gaagatcagg atcgacatgc
     1021 cgttgccgac cccgcgctgg gtgatcagct cgcccagcca catgatcagg gccgtccccg
     1081 cggtcagggt catcacggcc agggcgaacc ggccgggggt caacccgggg aagatgtcga
     1141 tcggcaggat cccctggttg ggggcgagct gcacgatgct ggccgactgg agcagggcca
     1201 ggaacaccgt cacgtagcgg gtgtactgct ggatcttctt gcgcccggcc tccccttcct
     1261 tctggagctg ctccagccgg gggatcacca ccccgagaag ctgcatgatg atcgaggagg
     1321 tgatgtaggg catgatcccc agggcgaaga tcgcgaactg ggtcagcgcc ccgcccgaga
     1381 acaggttgat cagcgtgaac gccccgctgg ggtcctgggc cgggttctcg aggtagttct
     1441 ggacggcgtc gaagtcgacc ccgggcaccg gcaggtgcga cccgagccgg tacacggcga
     1501 tgatcgccac ggtgaagagg atcttgcccc gcagctcggg gatcttgaag gcgttgcgaa
     1561 aggccgacag cacgcgggtt cctctccttt gcggcctaga cgacctctac ggtaccgcct
     1621 gcggcggtga tcttggaggc cgcagtggcc gagaaggcgt gggcccgcac ctcgaaggcg
     1681 accgagacgt cgccgtggcc gaggaccttg accggggtgc ccttgcgggc cagaccggcc
     1741 gcgaccagcc cgtccgggtc gacgccgccg cccctgggga acgcggcctg gaggcggtcg
     1801 aggttgacca ccgaccagtg gaccttgttg gggttggtga acccgcgcag cttggggacg
     1861 cggcgctgga gcggcatctg gccgccctcg aacccgatcc ggatctgcga ccgggccttg
     1921 gagcccttgg tgccccggcc ggccgtcttg cccttgcccg aggcatcgcc gcgccccttg
     1981 cgcacccggt ccttggtgga cccgggcgcc gggcgtaggt gatgcagctt cactcttgta
     2041 cctcctccac ctcgaccagg tgggagacct tgaagatcat gccgcggatc tcggggcggt
     2101 cctccttgac gacggtgtcg ccgatccgct tgagccccag gctcctgacc gtgtcccgct
     2161 gcgggcgggg ccggccgatc agggaccgct tctgggtgac cttgaggcgg gtgctcacgc
     2221 cgactgctcc ttcgccttgc cggcgcccgc gccgaccgcg gccgccgcct tctgcctggc
     2281 caccgtgacc ggcgacatct cggccagctc gaggccgcgc cggtcggcca cctcctgggg
     2341 ccgccggagc tgcttgaggc cggcgacggt ggcgtggacg atgttgatcg ggttggacga
     2401 cccgagcgac ttggagagga cgtcggagac cccggccgcc tcgaggacgg cccggaccgg
     2461 gccgccggcg atgaccccgg ttcccgggga ggccggcttg agcaggaccc ggccggcgcc
     2521 ctcggcgccg agcacctcgt gcgggatggt cgagccgatc atcggcaccg ggaagaagtt
     2581 cttcttggcc gtctcgaccc ccttctggat ggccgagggg acctccttgg ccttgccgta
     2641 gccgacgccg accttgccgt tgccgtcgcc
//
LOCUS       DASSWR010000242         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_179327,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000242 DASSWR010000000
VERSION     DASSWR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179327"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFO47_04120"
     CDS             complement(<1..368)
                     /locus_tag="VFO47_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET8787914.1"
                     /translation="MTDEELRDLLLDTKVIAVVGLSANPDRPSNQVAWYLHHQGYRLF
                     GVNPACPEPEVFGVPMLPSLDQVPEPIDLVDVFRRPEHTPQVAREAVAAGAGAVWLQL
                     GIRSDEARAIAEQGGLVYVE"
     gene            complement(469..984)
                     /locus_tag="VFO47_04125"
     CDS             complement(469..984)
                     /locus_tag="VFO47_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787915.1"
                     /translation="MTVAVQAHPSATREQLAAIREAIDTWAGVLDECFDGLITLTEVS
                     RRQAADIVVHYVPTAGGVVFGGYAICGDHGCPNILVRSDLPPSLDRDPYDPEYLGWVT
                     LHELGHALGLGHATNLLESYDLMGYGWPDLGHPVLSQCDVDALAFVFAWALEGAEPYP
                     PAEGPYDCSAA"
     gene            1961..>2363
                     /locus_tag="VFO47_04130"
     CDS             1961..>2363
                     /locus_tag="VFO47_04130"
                     /inference="COORDINATES: protein motif:HMM:NF026760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase N-terminal
                     domain-containing protein"
                     /protein_id="HET8787916.1"
                     /translation="MSQDTVRRLDDTDRERGTPHRGRLLRRPTFGGLVAALVFWWWSL
                     QPSMLPRTPSAQGAVSGLSAAVGYLLGTLAGYGVAALLRRRGREPTRRAGRVAWLALG
                     VVALVVVAAGLGQWTGWQNQQRDLVDMEHLSP"
BASE COUNT          329 a          837 c          892 g          305 t
ORIGIN      
        1 tcctcgacgt agaccaggcc gccctgctcg gcgatggcgc gggcctcgtc gctgcggatg
       61 ccgagctgga gccagacggc gccggccccg gccgcgaccg cctccctggc cacctggggg
      121 gtgtgctccg ggcggcggaa gacgtcgacg aggtcgatcg gctcgggcac ctggtcgagg
      181 gagggcagca tcggcacccc gaacacctcc ggctccgggc aggcggggtt gaccccgaac
      241 aggcggtagc cctggtgatg caggtaccag gcgacctggt tggaggggcg gtcggggttg
      301 gccgagaggc cgaccacggc gatcaccttg gtgtccaaca gcaggtcgcg gagctcttcg
      361 tcggtcatgg caggggcccc tggcatcggc ggcagacccg gccatggtag gcccgcagag
      421 gcggcaccgg agacaggtcc ggcgccgccc ctgggccctg gcctcagatc aggccgctga
      481 gcagtcgtac ggaccctcgg ccggcgggta gggctcggcc ccttccaggg cccaggcgaa
      541 cacgaaggcc agcgcgtcca cgtcgcactg cgacagcacc gggtgaccca gatcgggcca
      601 gccatagccc atcaggtcgt agctctccag caggttggtg gcgtgtccca gtcccagcgc
      661 gtggccgagc tcgtgcagcg tcacccagcc caggtactcg gggtcgtagg ggtcgcggtc
      721 caggctgggc ggcaggtccg agcggacgag gatgttgggg cagccatggt cgccgcagat
      781 ggcgtagccg ccgaacacca ccccgccggc ggtcggcacg tagtggacca cgatgtcggc
      841 ggcttggcgc cgtgagacct cggtcagggt gatgaggccg tcgaagcact cgtccagcac
      901 cccggcccat gtgtcgatgg cttcgcggat ggccgccagc tgctccctgg tggcgctcgg
      961 atgggcctgg acggcaacgg tcaagttggt gaggactcgc ccagccagct ggcgggctcc
     1021 cgccagggct tgatgccctc gtactccggg ttgtgctgca tggaacgaac ccgcctacgg
     1081 cgttcgcgtc gacccttggt cgagctcgcc gaggcgacca gcagcagccc gatgacgagg
     1141 ccgaggcccc gccgccgctg ccagcaacca gcccagggag gagccggaga ccaaccggtc
     1201 gagcgcccgg accagggaaa cggacagggc gggccgccga cgaccagccg acacgcattc
     1261 ctgtaaccac cgcacgtccg ggttcgttct tctttggtga agctcggtga gccggggaca
     1321 ggccagaggg aggcctggtc gcgccgcgaa gccatgggga tgacgcaagc ggcgcgcgat
     1381 atcaccctgc cgggtgacgg acaccccgct tcacctcgcg tggacctccg ccggctgatt
     1441 ggcggaggtc cacgcttttc ttctcccgcg ctggtcgtcc ccgttcccgg ctcctgagcc
     1501 cacccaggac gacgaccaag gcggcggcga ggaagaccac cccggcgcgg aggccccacc
     1561 ggagcccgtc gagcagggcg ccgagctcgt ccccgatccg ggcgaacgcg tcggcgcggg
     1621 cggtcggccc cgaggccctt gacgtcctcg tccaccccgg cggtggcggt gatcgtgatg
     1681 gccacgtcgg ccaccgcctt gccgacggcc cagaggacgc tgccggcggt cgccagcgcc
     1741 aggtcgccca ccggcagcag cgccatcacc agcaggccgg cggcctggac ggcgatcccg
     1801 agaaccatcg ccgggcgcgg cccggccagg gccgctgggg caccggcgcc atgccgttcg
     1861 gggtcggctt ccgcctgatc ggggcgacac ggcaaggcgc gacgatatcc tggaccaggg
     1921 caagcacggg ccgccgcgcg cgcccacttg ggggtgggac gtgagccagg acacggttcg
     1981 acgcctcgac gacaccgacc gcgagcgcgg gaccccgcac cggggcaggc tgctgcggcg
     2041 gccgacgttc ggcggcctgg tcgcggcgct ggtcttctgg tggtggtcgc tgcagccgag
     2101 catgctgccc cggacaccgt ccgcccaggg ggcggtgagc gggctgtcgg cggccgtcgg
     2161 gtacctgctg gggacgctgg ccggctacgg cgtggcggcg ctgctccgcc ggcgcgggcg
     2221 cgagccgacc cggcgggccg gacgcgtcgc ctggctcgcc cttggcgtgg tcgccctcgt
     2281 cgtggtcgcc gccgggctcg ggcagtggac gggatggcag aaccagcagc gcgacctggt
     2341 ggacatggag cacctgtcgc cgt
//
LOCUS       DASSWR010000243         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1252700, whole genome shotgun sequence.
ACCESSION   DASSWR010000243 DASSWR010000000
VERSION     DASSWR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252700"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VFO47_04135"
     CDS             complement(<1..1207)
                     /locus_tag="VFO47_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8787917.1"
                     /translation="MTTDGGPAGAGSLRLLGANRPFRLLWSARAVSFLGDSLGLVALM
                     LHVADTTGQALAVALLLLAGDFLPALAGPLAGTLADRLDRRRVMVGCELVQGSLVVVT
                     ALWLPPLPLLLVLVGLRALAGQVFQPASRAAVPAVVPERDLERANAAVGVATNGGETL
                     GPLVAALLLPVLDVRGVLLVDAATFLVSAVVLGFLPALPGAAPEDGRRPSFLADARAG
                     LRYLGSEATVRAVALGYFAIVACNGIDDVALVFLATDTLGAGAAAVGLLLAAVGIGLL
                     AGYALLARTRARVSMPALLLAGFAVSSAGNLLTGLAWAVAAAFAVQAVRGLGIAALDV
                     AATTLVQRTVPPHLLGRVFGSLYGGIGVAAALAYLLGGLLLDRTTPRVAFVAAGAAGL
                     LATGVTALAL"
     gene            1293..>2056
                     /locus_tag="VFO47_04140"
     CDS             1293..>2056
                     /locus_tag="VFO47_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8787918.1"
                     /translation="MAEVLVLSEADVAGLLDLEPLLAALGQALQSSSAGRATAPPRVA
                     TWAEGGGLGAMPVHLPGVALGAKLVSVFPANHERGRPSHQALIALFDPDDGTPVAVMD
                     GTRITAARTGATAAVAAGALARPDAAVLAILGAGVQGRSHLDAFPLVRDFRDIRVASR
                     TPGHADALAAAHPAARAVASFEEAVDGADVVCCCTDAREPILELAWLAPGTHLSSVGG
                     SFGPELDPATMRAGRVFVEWRGAAANPPPAGAHELQG"
BASE COUNT          262 a          819 c          795 g          180 t
ORIGIN      
        1 ggagggccag ggccgtgacg ccggtggcca gcaggccggc ggcgccggcg gcgacgaacg
       61 cgacccgggg cgtggtgcgg tccagcagga ggccgccgag gaggtaggcg agggcggcgg
      121 ccaccccgat cccgccgtag agactgccga acacccggcc gagcaggtgc ggcggcacgg
      181 tccgctggac cagggtggtg gcggccacgt cgagggcggc gatgccgagc ccgcgcaccg
      241 cctggacggc gaaggcggcc gcgaccgccc aggccaggcc cgtgagcagg ttgccggcgc
      301 tgctgacggc gaagccggcc agcagcaggg cgggcatcga gacccgggcg cgggtccggg
      361 ccagcagcgc gtacccggcc agcagcccga tgccgacggc ggccaggagc agcccgaccg
      421 cggctgcgcc ggcgcccagg gtgtcggtgg ccaggaagac cagggccacg tcgtcgatgc
      481 cgttgcaggc cacgatggcg aagtagccga gggcgaccgc ccgcaccgtc gcctcgctcc
      541 ccaggtaacg cagcccggcc cgggcgtcgg ccaggaacga cgggcgccgg ccgtcctccg
      601 gcgccgcccc tggcagcgcc ggcaggaacc cgagcacgac cgccgagacc aggaacgtgg
      661 ccgcgtcgac cagcagcacc ccgcggacgt ccagcaccgg cagcagcagc gccgcgacca
      721 gcgggccgag ggtctcgccg ccgttggtgg cgacgccgac ggccgcgttg gcccgctcca
      781 ggtcgcgctc gggcaccacc gcgggcacgg cggcccgcga cgccggctgg aacacctggc
      841 cggccagggc ccgcagcccg accaggacga gcagcagcgg cagcggcggc agccacaggg
      901 cggtcacgac caccagggac ccctggacca gctcgcagcc gaccatcacc cgccgccggt
      961 ccagccggtc ggccagggtc ccggcgagcg ggccggccag cgccggcagg aagtccccgg
     1021 ccagcagcag gagcgccacc gccagggcct gcccggtggt gtcggcgacg tgcagcatga
     1081 gggcgaccag gccgagcgag tcgcccagga aggacaccgc ccgggcgctc cacagcaggc
     1141 ggaacggccg gttggcgccg agcagccgca gcgacccggc cccggcgggt cccccgtcgg
     1201 tcgtcatggc agacgatcct ctcccatcgg ccctccaggc cggccggggt agattggccg
     1261 ccgtcgggtc ggcgcggcga cgggagcgga cggtggccga ggtgctggtg ctcagcgagg
     1321 cggacgttgc cggcctgctc gacctggagc cgctgctggc cgcgctcggc caggccctgc
     1381 agagctccag cgccgggcgg gccacggcgc cgccgcgggt cgcgacctgg gccgagggcg
     1441 gcgggctggg ggcgatgccg gtgcacctgc ccggggttgc cctcggggcc aagctggtca
     1501 gcgtcttccc cgccaaccac gagcggggcc gcccgtccca ccaggcgctg atcgcgctct
     1561 tcgaccccga cgacggcacc ccggtcgcgg tcatggacgg cacccgcatc accgccgccc
     1621 gcaccggggc cacggccgcg gtggcggccg gcgccctggc ccggcccgac gcggccgtgc
     1681 tggccatcct cggcgccggc gtccagggcc gctcccacct ggatgcgttc ccgctggtgc
     1741 gcgacttccg cgacatccgc gtggcctccc gcacccccgg ccacgccgac gccctggccg
     1801 ccgcccaccc ggcggcgcgg gcggtcgcct cgttcgagga ggcggtcgac ggggccgacg
     1861 tggtctgctg ctgcaccgac gcccgcgagc ccatcctgga gctcgcctgg ctcgcccccg
     1921 gcacccacct gagctcggtc gggggcagct tcggccccga gctcgacccg gccaccatgc
     1981 gcgccggccg ggtcttcgtc gagtggcgcg gcgccgccgc caacccgccc ccggccggcg
     2041 cccacgagct ccaggg
//
LOCUS       DASSWR010000244         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1281232, whole genome shotgun sequence.
ACCESSION   DASSWR010000244 DASSWR010000000
VERSION     DASSWR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281232"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFO47_04145"
     CDS             complement(<1..622)
                     /locus_tag="VFO47_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8787919.1"
                     /translation="MAARSLTVGTVKITAHCDVTGDFPAPLAEAFPQVPAAAWAPWRS
                     RHPGAFGGPDRWRLRDWCFVVRSRDLVVLVDTGVGGPGVPGAGWIGTPGRLPAELADA
                     GVEPDEIDLVVLTHLHLDHVGWNLAWDGDRPRPRFPRARYLVQRADWDLFAARPAETR
                     VAFDRCVAPLQDLGVAELLDGDRALDAQLRLVHTPGHTPGSQSLLVR"
     gene            complement(632..1531)
                     /locus_tag="VFO47_04150"
     CDS             complement(632..1531)
                     /locus_tag="VFO47_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8787920.1"
                     /translation="MSRRLLETGRGSRPGSFGAAEWALLVSVALIWGSSFLFIDIGLE
                     TLEPGVIAVARVALGVAALALVPAARRPIGRDDRLRVAFLGIIWSGLPLILFPVAQQW
                     IDSSVAGMLNGAVPLAGAAWAVVLLRRPLPRVHALGLLIGFAGVLAISWPQLAGSRAT
                     MLGASLVVLAVLCYGLAVNLAVPLQQRHGALPVLLRAQLAALVLLLPFGLWGLRGSSF
                     AWPSVLAMLPLGILGTGLAFVLMTTLVGRVGGPRGAVATYFIPVVAIVLGVLVLDEQV
                     APSAVAGTALVVTGAWLTSRPER"
     gene            complement(1528..2697)
                     /locus_tag="VFO47_04155"
     CDS             complement(1528..2697)
                     /locus_tag="VFO47_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004041010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8787921.1"
                     /translation="MTGEGPRLDAAGDFGGFDGRAWLNCAHQGTLPRVAVAAAERALA
                     DKAAPHRIRDEAFDAVPRRLKDALGRLAGVPADQVTLGNSTSYGLHLLVQGVPWREGD
                     EVLLVDGDFPATIVPWLPLADRGVRVRLLKPEGGRLEAGQLEAELTPATRLFCTSWVF
                     SFTGHTVDLTAVGRVCRRAGVTFVVNGSQAVGAVPLDLAGLPVDALVSCGFKWLCGPY
                     GTGFCWLAPDLLASLTDRPAYWLAHQGQDDLGQDSGYRLREDVGAAGWDVFGTANFLT
                     FQPWTASVEYLLGLGIDRVAAWDQHLVDRFVGGLDPDRYELVSPGRGPARSALVVFGD
                     HRPEGNPPLHQALTAAGVDAAFRRGRLRVSPHLYNTEADIDRALEVLDGAPGGRP"
     gene            complement(2694..3803)
                     /locus_tag="VFO47_04160"
     CDS             complement(2694..3803)
                     /locus_tag="VFO47_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HET8787922.1"
                     /translation="MTDLAASEGRTSGITMEELALAARNHGMPLEALRYDITPVGLHY
                     LLIHFDIPAADERTWRLEIGGRVATPISLSMEELRRRPAVTAPVTLECAGNGRARLDP
                     RPVSQPWLEEAVGTAEWTGTPLAPLLEEAGVGPGAVEVAFAGADRGVQGGLEHDYERS
                     LPLAEAMRPEILLAWAVNGRPLPPQHGHPLRLVVPGWYGMTSVKWLRRISVLGEPFEG
                     YQQTGTYLIHASEDDPGTPVTRIQPRSLLLPPGIPEFESRRRFLAPGRHLLTGRAWSG
                     LAPVAAVEVSSDGGRTWRPAELDGQPSPWAWAGWRFAWEAAPGSYELCSRATDAAGNT
                     QPLEAPWNTGGYVNNAVQRVQVTVRDPADAAAGPA"
     gene            complement(3819..4004)
                     /locus_tag="VFO47_04165"
                     /pseudo
     CDS             complement(3819..4004)
                     /locus_tag="VFO47_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4078..>4258)
                     /locus_tag="VFO47_04170"
     CDS             complement(4078..>4258)
                     /locus_tag="VFO47_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8787923.1"
                     /translation="FIIAAADTVMRRPSRELMAEVFPGVPVKDDLATHGTLLGIDKAR
                     RLLGYDPEFTWRSLL"
BASE COUNT          587 a         1546 c         1668 g          457 t
ORIGIN      
        1 agcggacgag caggctctgc gagccgggcg tgtgcccggg ggtgtggacg aggcggagct
       61 gggcgtccag ggcccggtcg ccgtccagca gctcggccac cccgaggtcc tggagcgggg
      121 ccacgcagcg gtcgaaggcc accctggtct cggccggccg ggccgcgaac aggtcccagt
      181 cggcccgctg gaccaggtag cgggcccgcg ggaaccgcgg ccggggacgg tcgccgtccc
      241 aggccaggtt ccagccgacg tggtccaggt gcaggtgggt gaggacgacc aggtcgatct
      301 cgtccggctc gaccccggcg tcggccagct cggcgggcag ccgtcccggc gtgccgatcc
      361 agccggcccc gggcacgccc gggccgccga cgccggtgtc gaccaggacg accaggtcgc
      421 gggagcggac cacgaagcac cagtcgcgca gccgccatcg gtcggggccg ccgaacgccc
      481 cgggatggcg cgaccgccag ggcgcccagg cggccgccgg gacctggggg aaggcctcgg
      541 ccaggggggc cgggaagtcg ccggtgacgt cgcagtgggc ggtgatcttg acggtgccga
      601 cggtcagcga ccgggcggcc atgggcctcc ttcagcgctc ggggcgggag gtcagccagg
      661 cgccggtgac caccagggcg gtgccggcga cggccgaggg ggccacctgc tcgtcgagca
      721 cgagcacgcc gaggacgatg gccacgaccg ggatgaagta ggtggcgacg gcccctcggg
      781 ggccgccgac gcggccgacc agggttgtca tcaggacgaa ggcgagcccg gtgccgagga
      841 tccccagggg cagcatggcc agcaccgacg gccaggcgaa cgaggagccg cgaagccccc
      901 agagaccgaa cggcagcagc aggaccagcg cggccagctg ggcccggagc agcaccggca
      961 gggcgccgtg gcgctgctgc aggggcacgg ccaggttgac ggccaggccg tagcagagga
     1021 cggccagcac gaccaggctg gccccgagca tggtggcccg cgagccggcc agctggggcc
     1081 aggagatggc cagcacgccg gcgaacccga tgaggagccc gagggcgtgg acgcggggca
     1141 ggggccggcg cagcagcacc accgcccagg cggccccggc cagcggcacg gccccgttga
     1201 gcatcccggc caccgacgag tcgatccact gctgggccac cgggaacagg atcagcggca
     1261 gcccggacca gatgatcccg aggaaggcca cccgcaggcg gtcgtcgcgg ccgatcgggc
     1321 ggcgggccgc cgggaccagg gcgagtgccg ccacgccaag ggcgacccgg gcgacggcga
     1381 tcacccccgg ctccagcgtc tccagcccga tgtcgatgaa caggaacgac gacccccaga
     1441 tgagggccac gctgaccagc agggcccact cggcggcgcc gaaggacccg gggcggctgc
     1501 cccgcccggt ctccagcagc cgccggctca cggccgcccc ccgggggcgc cgtcgaggac
     1561 ctcgagggcg cggtcgatgt cggcctcggt gttgtagagg tgcggggaga cccgcagccg
     1621 cccccgccgg aaggccgcgt cgaccccggc ggcggtcagc gcctggtgca gtggcgggtt
     1681 gccctcgggc cggtggtcgc cgaagacgac cagggccgag cgggccggcc cccgcccggg
     1741 gctgaccagc tcgtagcggt ccgggtcgag acccccgacg aaccggtcga ccaggtgctg
     1801 gtcccaggcg gcgacccggt cgatgccgag gccgaggagg tactcgaccg aggccgtcca
     1861 gggctggaag gtgaggaagt tggccgtgcc gaacacgtcc cagccggcgg cgccgacgtc
     1921 ctcgcggagc cggtagccgc tgtcctggcc gaggtcgtcc tggccctggt gggccagcca
     1981 gtaggccggc cggtcggtga gcgaggccag caggtcggga gccagccagc agaagccggt
     2041 cccgtacggg ccgcagagcc acttgaaccc gcagctgacc agcgcgtcca ccggcagccc
     2101 ggccaggtcc aggggaacgg ccccgacggc ctgggagccg ttgacgacga aggtcacccc
     2161 ggcccggcgg cacacccgac ccaccgcggt caggtcgacc gtgtgcccgg tgaaggagaa
     2221 cacccagctg gtgcagaaca gccgggtcgc cggggtcagc tccgcctcga gctgcccggc
     2281 ctccagcctc ccgccctcgg gcttgagcag ccggacccgc accccgcggt cggccagggg
     2341 cagccagggc acgatggtgg ccgggaagtc cccgtcgacc agcaggacct cgtcgccctc
     2401 gcgccagggg acgccctgga cgagcaggtg cagtccgtag ctggtgctgt tgcccagggt
     2461 cacctggtcg gccggcaccc cggcgagccg cccaagggcg tccttgagcc gccgtgggac
     2521 cgcgtcgaag gcctcgtcgc ggatccggtg cggcgccgcc ttgtcggcca gcgcccgctc
     2581 ggccgccgcc accgcgaccc ggggcagcgt cccctggtgg gcgcagttca gccaggcccg
     2641 cccgtcgaac ccgccgaagt ccccggcggc gtccagccgg ggcccttcgc cggtcaagcc
     2701 ggtccggccg cggcgtcggc ggggtcgcgg acggtgacct ggacccgctg gacggcgttg
     2761 ttgacgtagc cgccggtgtt ccagggggcc tcgaggggct gggtgttgcc ggcggcgtcg
     2821 gtggcccgcg agcacagctc gtacgacccg ggggccgcct cccaggcgaa gcgccacccg
     2881 gcccaggccc agggggacgg ctggccgtcc agctcggccg gccgccaggt gcggccgccg
     2941 tcgctgctga cctcgacggc cgccaccggg gccaggcccg accaggcccg gccggtcagc
     3001 aggtggcggc cgggggcgag gaagcgccgg cgggactcga actcggggat gccgggcggc
     3061 agcagcagcg agcgcggctg gatgcgggtc acgggcgtgc ccgggtcgtc ctcggaggcg
     3121 tggatcaggt aggtgccggt ctgctggtag ccctcgaacg gttcgccgag cacgctgatc
     3181 cggcggagcc atttgaccga ggtcatgccg taccagccgg gcacgaccag ccgcagcggg
     3241 tggccgtgct ggggtggcag gggccggccg ttgaccgccc aggcgagcag gatctcgggg
     3301 cgcatggcct cggccagggg caggctgcgc tcgtagtcgt gctcgagccc gccctggacg
     3361 ccccggtcgg cgccggcgaa cgcgacctcg acggctcccg ggccgacccc ggcctcctcc
     3421 agcagggggg ccagcggcgt gccggtccac tcggccgtcc ccaccgcctc ctccagccag
     3481 ggctggctga ccggccgcgg gtcgaggcgg gcccggccgt tgccggcgca ctctagggtc
     3541 accggcgccg tcaccgccgg acgccgccgg agctcctcca tggagaggct gatcggcgtg
     3601 gccacccgcc cgccgatctc cagccgccag gtccgctcgt cggccgccgg gatgtcgaag
     3661 tggatcagca ggtagtggag gccgaccggg gtgatgtcgt agcgcagcgc ctccagcggc
     3721 atgccgtggt tgcgtgccgc cagggccagc tcctccatgg tgatgccgct cgtgcgacct
     3781 tcgctggccg ccaggtcggt catggtgatc ctctccttct actggaggtc gagaccgcgg
     3841 aggagctggg cgttgagggc gacgacgatg gtggacaggc tcatcaggat ggcgccgacg
     3901 gccggggcca gggtgatgcc ggcccaggcc agggcgccgg cggcgaccgg cacggccacc
     3961 aggttgcagg agccggtcgc ccaccccaga ttctgcagca tcttgccgca gccgtcccct
     4021 ccccccccgc ctccacccgg gcccggaccg ccgtgtcggg cggggtgaag tggtccatca
     4081 gagcagcgat ctccaggtga actcggggtc gtagcccagc agccgcctcg ccttgtcgat
     4141 gccgaggagg gtgccgtggg tggccaggtc gtccttgacg ggtaccccgg gaaacacctc
     4201 ggccatcagc tcgcggctgg gacggcgcat gacggtgtcg gcggcggcga tgatgaac
//
LOCUS       DASSWR010000245         5825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_828407,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000245 DASSWR010000000
VERSION     DASSWR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828407"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFO47_04175"
     CDS             <1..729
                     /locus_tag="VFO47_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787924.1"
                     /translation="RAVELLDIVGIPNAPERARNYPHEFSGGMRQRAVIAMSMTNDPD
                     VIIADEPTTALDVTVQAQVLDTLRRVQENTSAGIVLITHDLGVVASMAHRVLVMYAGK
                     PVEIGETDSIYYDPRMPYTLGLLGSLPRLDQQADEPLTPIRGAPPSLINLPPGCPFSP
                     RCPMSRPHCDAEEPPLRSVSGVDHVAACHYAEELAGDVAAAEVFETAETDTQAIEQLD
                     QLGRLDEAKAELDQAEAAEEAERA"
     gene            726..1757
                     /locus_tag="VFO47_04180"
     CDS             726..1757
                     /locus_tag="VFO47_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET8787925.1"
                     /translation="MTQTTPQPAATQDGAKDPILEVTNLVKHYPLMGGGVVRRRVGDV
                     HAACDLTFHLDRGETLGLVGESGCGKSTTGKAILQLSPPTSGSVKFQGEELVGKTRKQ
                     MRSIRRDLQVVFQDPYASLNPRIPVNDIVAEPLIIHGLFKRQGRERVRELLTVVGLNP
                     EHGNRYAHEFSGGQRQRIGIARALALEPKVLVLDEPVSALDVSIQAGVVNLLDELQDR
                     LGLSYVFIAHDLSVVRHICDRVAVMYLGKIVEIGSRDHIYERASHPYTQALLSAVPIP
                     DPKLERQRQRIVLEGDVPSPVNPPSGCRFRTRCWKAQDICAEQEPELVDRGQGHPVAC
                     HFAEPRSLL"
     gene            1797..3521
                     /locus_tag="VFO47_04185"
     CDS             1797..3521
                     /locus_tag="VFO47_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8787926.1"
                     /translation="MKLVRWVSLLAALVLLAAACDDGGGVGVGDGEPNRGVKRGTLRV
                     FSYSPADTLDPGIAYFDLDFALFRGMVRELYSFDTRVEGERSLQPVPDLADGPYQLSD
                     DGRTYTFKLRRGVRYAPPVDREVQAKDFVYAIERQVDPKLAPRHRSPNPYARLISGVD
                     DFAAGKARRISGLRTEGDHVLHITLDRPASDFPSILTLPFFSPVPKEHASEYTPGDSY
                     GQHLVASGPYRLKQHDPGRLIELVRNENWDPRTDPLRSAWVDRITVYIGRSESDIQQA
                     IEEGEADLNLDAVPPPVADLPRLSADPTLARQLAVETTGCVHYLTMQTDAGPTRSLAV
                     RQAVNYAIDRQALVLAMGGRYAGEPATTVLSPTLAGYSAFDLYPSQDFRGDPDRARKL
                     LAEAGYPNGVDLTYAGQNSPKWKAMYKALRASLERAQITLEAKFYKGREAYSRSLRLR
                     SKRDEHHLGAAAVCPDVPGNGSRSIIGVLLDGRLISPTANNNYGNYNNDKVNALIDQA
                     YEARDDQSRAALWGQVDRLVMEDAAWAPLVYDREAFFWSSRVRNWTFSPWLKNPDITN
                     LWLDPNTP"
     gene            3521..4897
                     /locus_tag="VFO47_04190"
     CDS             3521..4897
                     /locus_tag="VFO47_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8787927.1"
                     /translation="MDDVFAHVDANRDAFVQRLAGWVRRPSVSATGQGMPEAAGHARD
                     LVEAAGLAATVEPTGGWPLVVGHRAGPPGAPAVLVYGHYDVQPPDPLDAWTSPPFGPK
                     VRDGRLYGRGTADNKGQHLAQHLAMESLLATRGGLPCTVKVLLDGEEEIGSPNLAAFA
                     QRNRDRLAADLVVWSDGPVDPAGGWRLVFGVRGIASFELRARGANRSLHSGNWGGVAP
                     NPLWTLVHLLASMRDADGRITVEGFGDEVEPLGSEERAALERLPVDVEAVKAELGLAE
                     LDAPAGRGFAERLAAWPTLTINGLHGGYGGEGTQTVLPSAAVAKCDVRLVHAQRAGDV
                     YEKLEAHVRRHAPGVELVRQGSMEPSRTPMDSPFTEPIRAGMAAAQGADPLLVPVLGG
                     SLPLHVFTGVLGLPTFGIPLGNPDQANHAPDENLDLDRFHTGVKTAAAVLAKLGSSEG
                     AASGTMAG"
     gene            4894..>5825
                     /locus_tag="VFO47_04195"
     CDS             4894..>5825
                     /locus_tag="VFO47_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET8787928.1"
                     /translation="MTTSAPQGRLLRRGLRVIGRQVALHPRPFVIAVLGAMVYAVGTV
                     AQSWVLGQVVDRAVTPRFEAGQLRAGAAVAAACAIVAVGLVKTAGIITRRIAATTVHA
                     NVQATLRDRLAERYHRLPLTWHHEHPAGELLSRAEGDLDAASEILSPLPFATGVVTML
                     GAAAVWLFLADPFLATLGMLVVPAIVVLNLAFQRRVEHPTTLGQERLGDMSAVAHESF
                     DGALVVKALQAEEAEGRRFADKAELLRDARIEAARVTANFEAGLDALPAVTTVALLVI
                     GVWRIEQGAITAGTLVGLINLFTLLIWPLRLIGYV"
BASE COUNT          826 a         2254 c         1998 g          747 t
ORIGIN      
        1 cgggcggtgg agctgctcga catcgtgggc atccccaacg cccccgagcg ggccaggaac
       61 tacccgcacg agttctccgg cggcatgcgc cagcgggccg tcatcgccat gtcgatgacc
      121 aacgacccgg acgtgatcat cgccgacgag cccaccaccg ccctggacgt gaccgtccag
      181 gcccaggtgc tcgacaccct gcgccgagtg caggagaaca cctcggccgg gatcgtgctc
      241 atcacccacg acctcggggt ggtggccagc atggcccacc gggtgctggt catgtacgcc
      301 ggcaagccgg tggagatcgg cgagaccgac tcgatctact acgacccgag gatgccctac
      361 accctgggcc tgctggggag cctgccccgc ctcgaccagc aggccgacga gcccctcacc
      421 ccgatccggg gggcgccgcc gtcgctgatc aacctgcccc cgggctgccc gttctcgccc
      481 cgctgcccga tgagccgccc ccactgcgac gccgaggagc cgccgctgcg gtcggtgagc
      541 ggcgtggacc acgtcgccgc ctgccactac gccgaggagc tggccgggga cgtcgccgcc
      601 gccgaggtgt tcgagaccgc cgagaccgac acccaggcca tcgagcagct cgaccagctc
      661 ggccgcctgg acgaggccaa ggccgagctc gaccaggccg aggcggcgga ggaggcggag
      721 cgcgcatgac ccagaccacc ccccagccgg ccgcgaccca ggacggcgcc aaggacccga
      781 tcctcgaggt caccaacctg gtcaagcact acccgctgat gggcgggggc gtggtccggc
      841 gccgggtcgg cgacgtccac gcggcctgcg acctgacctt ccacctggac cggggcgaga
      901 cgctcgggct ggtgggcgag tcgggctgtg gcaagtcgac caccggcaag gccatcctcc
      961 agctgagccc gccgacctcg gggtcggtca agttccaggg cgaggagctg gtcggcaaga
     1021 cccgcaagca gatgcggagc atccgccgcg acctccaggt ggtgttccag gacccgtacg
     1081 cctccctgaa cccgaggatc ccggtcaacg acatcgtggc cgagccgctg atcatccacg
     1141 ggctgttcaa gcgccagggc cgcgagcggg tccgggagct gctgaccgtg gtcggcctca
     1201 accccgagca cggcaaccgc tacgcgcacg agttctccgg cggccagcgc cagcgcatcg
     1261 gcatcgccag ggccctcgcc ctggagccca aggtcctggt cctcgacgag ccggtgtcgg
     1321 ccctggacgt gtcgatccag gccggggtgg tcaacctgct cgacgagctg caggaccggc
     1381 tcggcctgtc gtacgtgttc atcgcccacg acctgtcggt ggtgcgccac atctgcgacc
     1441 gggtcgccgt catgtacctg ggcaagatcg tcgagatcgg ctcccgcgac cacatctacg
     1501 agcgggccag ccatccctac acccaggcgc tgctgtcggc cgtgcccatc cccgacccca
     1561 agctggagcg gcagcggcag cgcatcgtgc tcgagggcga cgtgcccagc ccggtgaacc
     1621 cgccgtccgg ctgccgcttc cggacccgct gctggaaggc ccaggacatc tgcgccgagc
     1681 aggagccgga gctggtcgac cgcgggcagg gccacccggt ggcctgccac ttcgccgagc
     1741 cgcgcagcct gctctagcca ggcattcgcc gcccgggaac ggcatcatgt cgtgagatga
     1801 agctcgtcag gtgggtgtcg ctgctcgccg ccctcgtcct gctggccgcg gcctgcgacg
     1861 acggcggcgg tgtcggcgtc ggcgacggcg agccgaaccg gggggtcaag cggggcaccc
     1921 tgcgggtctt cagctacagc cccgccgaca ccctcgaccc gggcatcgcc tacttcgacc
     1981 tcgacttcgc cctgttccgg gggatggtca gggagctgta ctcgttcgac acccgggtcg
     2041 agggcgagcg ctcgctccag cccgtgcccg acctggccga cgggccctac cagctctcgg
     2101 acgacggccg cacctacacc ttcaagctcc gccgcggggt caggtacgcc cccccggtgg
     2161 accgcgaggt ccaggccaag gacttcgtct acgccatcga gcgccaggtc gatcccaagc
     2221 tcgcccccag gcaccgctcg cccaacccgt acgcgcggct gatcagcggg gtggacgact
     2281 tcgcggccgg caaggccagg cggatctcgg gcctgcggac cgagggcgat cacgtccttc
     2341 acatcaccct cgaccggccg gccagcgact tccccagcat cctcaccctg ccgttcttct
     2401 cgccggtgcc gaaggagcac gcctcggagt acaccccggg cgactcctac ggccagcacc
     2461 tggtggcgtc cgggccctat cggctcaagc agcacgaccc gggccggctc atcgagctgg
     2521 tccgcaacga gaactgggac ccgcggaccg acccgctgcg cagcgcctgg gtcgacagga
     2581 tcaccgtcta catcggccgc tccgagtccg acatccagca ggccatcgag gaaggggagg
     2641 ccgacctcaa cctcgacgcc gtgcccccgc ccgtggccga cctcccgcgg ctgtccgccg
     2701 acccgacgct ggcccggcag ctggcggtcg agacgaccgg ctgcgtccac tacctgacca
     2761 tgcagaccga cgccgggccg accaggtcgc tggccgtccg ccaggcggtc aactacgcca
     2821 tcgaccggca ggcgctggtc ctggccatgg gcggccgcta cgccggcgag ccggccacca
     2881 ccgtcctctc ccccaccctg gccgggtaca gcgccttcga cctctacccc tcccaggact
     2941 tccggggcga ccccgacagg gcccgcaagc tgctggccga ggccggctac cccaacggcg
     3001 tggacctgac ctacgccggc cagaactcgc ccaagtggaa ggcgatgtac aaggcgctgc
     3061 gggcgtcgct ggagcgggcc cagatcaccc tggaggcgaa gttctacaag gggcgggagg
     3121 cgtacagccg gtcgctgcgg ctgcggtcca agcgcgacga gcaccacctg ggcgcggccg
     3181 ccgtctgccc cgacgtgccc ggcaacggca gccgctcgat catcggcgtc ctgctcgacg
     3241 gccgcctgat ctccccgacc gccaacaaca actacggcaa ctacaacaac gacaaggtca
     3301 acgccctgat cgaccaggcc tacgaggccc gcgacgacca gtcccgggcc gccctctggg
     3361 gccaggtcga ccggctggtc atggaggacg cggcctgggc gccgctggtc tacgaccggg
     3421 aggcgttctt ctggtcctcc cgggtccgga actggacctt cagcccgtgg ctgaagaacc
     3481 ccgacatcac caacctctgg ctcgacccca acaccccctg atggacgacg tcttcgccca
     3541 cgtcgacgcc aaccgggacg cgttcgtgca gcggctggcc ggctgggtcc ggcggcccag
     3601 cgtgagcgcc accggccagg gcatgcccga ggcggccggc cacgcccgcg acctggtcga
     3661 ggccgccggc ctggcggcga cggtggagcc caccggcggc tggccgctgg tcgtcggcca
     3721 ccgcgccggc ccgcccggcg ccccggccgt gctcgtctac ggccactacg acgtgcagcc
     3781 gcccgacccg ctggacgcct ggaccagccc gccgttcggg cccaaggtcc gcgacggccg
     3841 cctctacggc cgcggcaccg ccgacaacaa gggccagcac ctggcccagc acctggccat
     3901 ggagagcctg ctggccacca ggggcgggct cccctgcacg gtcaaggtgc tgctggacgg
     3961 cgaggaggag atcggctcgc ccaacctggc cgccttcgcg cagcgcaacc gcgaccggct
     4021 cgccgccgac ctggtcgtct ggagcgacgg gccggtggac ccggccggcg gctggcggct
     4081 ggtgttcggg gtcaggggca tcgccagctt cgagctgcgg gcccggggcg ccaaccggtc
     4141 cctgcactcc ggcaactggg gcggggtcgc ccccaacccg ctgtggacgc tggtccacct
     4201 gctggcctcg atgcgggacg ccgacgggcg catcacggtc gaggggttcg gcgacgaggt
     4261 ggagccgctg ggctcggagg agcgggcggc ccttgaacgg ctgccggtcg acgtcgaggc
     4321 cgtcaaggcc gagctgggcc tggccgagct ggacgccccg gccgggcggg gcttcgccga
     4381 gcggctggcc gcctggccga ccctgaccat caacggcctc cacggcggct acggcggcga
     4441 ggggacccag accgtcctgc cgtcggcggc cgtggccaag tgcgatgtgc gcctggtcca
     4501 cgcccagcgg gccggcgacg tctacgagaa gctggaggcg cacgtccgcc gccacgcccc
     4561 cggggtcgag ctcgtccgcc agggctccat ggagccgtcg cggaccccga tggactcgcc
     4621 cttcaccgag ccgatccggg ccggcatggc cgccgcccag ggggccgacc cgctgctcgt
     4681 ccccgtgctc ggaggcagcc ttcccctgca cgtgttcacc ggcgtcctcg gcctgcccac
     4741 cttcgggatc ccgctcggca accccgacca ggccaaccac gcccccgacg agaacctcga
     4801 cctggaccgg ttccacacgg gcgtcaagac ggccgccgcg gtgctggcga aactcggttc
     4861 ctcggagggg gcggcttccg gcaccatggc cgggtgacca cctcagcccc ccagggacgc
     4921 ctgctccgcc gcggcctgcg cgtgatcggc cggcaggtcg cgctccatcc ccggccgttc
     4981 gtgatcgcgg tgctcggggc gatggtgtac gccgtcggca cggtggccca gtcgtgggtg
     5041 ctgggccagg tggtcgaccg ggcggtcacg ccccgcttcg aggcgggcca gctccgcgcc
     5101 ggggcggccg tggccgccgc ctgcgcgatc gtggccgtcg ggctggtcaa gacggccggc
     5161 atcatcaccc gccggatcgc cgccaccacc gtccacgcca acgtccaggc gaccctgcgc
     5221 gaccggctgg ccgagcgcta ccaccgcctg ccgctgacct ggcaccacga gcacccggcg
     5281 ggcgagctgc tgtccagggc cgagggcgac ctggacgccg cctccgagat cctctcgccg
     5341 ctgccgttcg ccaccggggt ggtgaccatg ctgggcgcgg ccgcggtctg gctgttcctg
     5401 gccgacccgt tcctggccac cctcggcatg ctcgtggtcc cggccatcgt ggtgctcaac
     5461 ctggccttcc agcgccgggt ggagcacccg accaccctcg gccaggagcg cctcggcgac
     5521 atgtcggcgg tggcccacga gagcttcgac ggcgccctgg tggtcaaggc cctccaggcc
     5581 gaggaggccg agggccggcg cttcgccgac aaggccgagc tgctgcgcga cgcccgcatc
     5641 gaggccgccc gggtcacggc caacttcgag gccggcctcg acgccctgcc ggccgtgacc
     5701 acggtggccc tgctggtgat cggcgtctgg cggatcgagc agggcgccat caccgccggc
     5761 accctggtcg ggctgatcaa cctgttcacc ctgctgatct ggccgctgcg gctgatcggc
     5821 tacgt
//
LOCUS       DASSWR010000246         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1252787, whole genome shotgun sequence.
ACCESSION   DASSWR010000246 DASSWR010000000
VERSION     DASSWR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252787"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFO47_04200"
     CDS             complement(<1..1273)
                     /locus_tag="VFO47_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787929.1"
                     /translation="MLVVGPGPAYLAFIRDVLPGLGEVDVAQRSVEDLVAEVTVTGTD
                     SPEVAAVKADPRMAAVIRRAAFLQVRPPALPLEVRWGHRVVRLPARMLRQRVDELVAG
                     DLRYQVGRDHLRDWLVERCLRHLEHTEGVSALDDPAAVQRSLGARPEVRAFLKRTWPV
                     VEPLRLVFRLLTEQPFLAEAAGGILSDQEQELLLWARRPRSLKAAAWSVTDAFLVDEA
                     VDVVGGTRTFGHVVADEAQDLSAMQLRAIGRRCRFGSATLLGDLAQRTTPWSAGRWGD
                     AVGHLGQRSVRVEHLPRAFRVPKEVLDFANRLLPEIASEVRPARSVRSVPGSLRVRAV
                     APAAVPGAWLRALEEALAEDGSVGLVVADLAVPAAGAELAGRGVEFREVDRFDADARL
                     ALVPASAVKGLEFDQVVLVEPADIADPALGAA"
     gene            complement(1304..1942)
                     /locus_tag="VFO47_04205"
     CDS             complement(1304..1942)
                     /locus_tag="VFO47_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787930.1"
                     /translation="MTDDELVAEREMLEYARRCLAAMRRRTRSLRAAGADAYAAESVE
                     WRLRQRVAALADDERTGLFFGRLDYDAGADPPEARFYVGRRHVSDAEGDPVVIDWRAG
                     LAHPFYRASPTHRFGVRLRRRFGYRGGTLTSIQDEVLDLLDEAEARAVADRVLVAEIE
                     RPRIGPMRDIVATIQPEQDDLIRAPLAETICIQGGPGTGKTAVGLHRAAFLL"
BASE COUNT          298 a          908 c          834 g          244 t
ORIGIN      
        1 cggccgcccc gagcgcgggg tcggcgatgt cggccggctc cacgagcacg acctggtcga
       61 actccaggcc cttgaccgcc gaggcgggaa cgagcgccag ccgggcgtcg gcgtcgaacc
      121 ggtccacctc ccggaactcg acgccgcggc cggccagctc ggccccggcg gccggcacgg
      181 ccagatcggc cacgaccagg ccgaccgacc cgtcctcggc cagggcctcc tccagcgccc
      241 gcagccacgc ccccggcacg gccgcgggcg ccaccgcccg gacccgcagc gacccgggga
      301 cgctccggac cgaccgggcc gggcggacct cggaggcgat ctcgggcagc aggcggttgg
      361 cgaagtcgag cacctccttg ggcacccgga acgcccgtgg caggtgctcg acccgcaccg
      421 agcgctggcc gagatggccg acggcgtccc cccagcgccc ggccgaccac ggcgtcgtcc
      481 gctgggccag gtcgccgagc agcgtcgccg acccgaaccg gcaccgccgc ccgatggccc
      541 gcagctgcat cgccgagagg tcctgggcct cgtcggccac cacgtggccg aaggtgcgcg
      601 tcccgccgac cacgtcgacg gcctcgtcga ccaggaacgc gtcggtcacc gaccaggccg
      661 cggccttgag gctccgcggc cgtctcgccc acaggagcag ctcctgctcc tggtcgctga
      721 ggatgccgcc ggccgcctcg gccaggaacg gctgctcggt gagcagccgg aacaccagcc
      781 ggaggggctc gaccaccggc caggtccgct tcaggaaggc ccggacctcg gggcgggcgc
      841 cgaggctgcg ctggaccgcg gccgggtcgt cgagtgcgct cacgccctcg gtgtgctcca
      901 ggtggcgcag gcagcgctcg accagccagt cccgcaggtg gtcgcggccg acctggtagc
      961 ggaggtcccc ggcgaccagc tcgtccaccc gctggcgcag catcctggcc gggaggcgga
     1021 ccacccgatg cccccagcgg acctcgagag gcagcgccgg cggccgcacc tggaggaacg
     1081 cggcccgccg gatgaccgcc gccatccgcg ggtcggcctt gaccgccgcc acctccgggc
     1141 tgtcggtccc ggtcaccgtg acctcggcga ccaggtcctc gaccgagcgc tgggcgacgt
     1201 cgacctcgcc gagaccgggc agcacgtcgc ggatgaaggc caggtaggcc gggccggggc
     1261 ccaccaccag caccccgtcc ctggccagcc ggtcgcggtg ctcttagagc aggaacgcgg
     1321 cccggtggag gccgaccgcg gtcttgccgg tgccgggccc gccctggatg cagatcgtct
     1381 cggccagcgg cgcccggatc aggtcgtcct gctcgggctg gatggtggcg acgatgtcgc
     1441 gcatcggccc gatgcgaggc cgctcgatct cggccaccag gacccggtcg gccacggccc
     1501 gggcctcggc ctcgtccagc aggtcgagga cctcgtcctg gatcgaggtc agggtcccgc
     1561 cccggtaccc gaacctgcgc cggagccgga ccccgaagcg gtgggtcggg ctggcccggt
     1621 agaagggatg ggcgagcccg gcccgccagt cgatcaccac cgggtcgccc tcggcgtcgc
     1681 tgacgtgccg gcgccccacg tagaaccgcg cctccggcgg gtcggcgccg gcgtcgtagt
     1741 ccagccgccc gaagaacagc ccggtccgct cgtcgtcggc cagcgcggcc acccgttgcc
     1801 gcagccgcca ctccaccgac tcggccgcgt acgcgtcggc cccggcggcc cgcaacgacc
     1861 gcgtccgccg ccgcatcgcc gccaggcacc ggcgggcgta ctccagcatc tcccgctcgg
     1921 cgaccagctc gtcgtcggtc aacggctcca gcggcccgct cacagggttc accaccaggt
     1981 tcgggttcgt gaaggagtcg ccgcagggcc gggcccggca cggctccacg gggacttgca
     2041 gcccacgcgg caccgttctg gacccgcggc aacagcccac catcctccat cgctcccggt
     2101 cgctgccgca acgcaatatc gccgaccgcg tgctggaggc gagccgttca gcggtgggcc
     2161 gggagcgccg gaggcggaga cctcagccga gggtcaggga gagctgggtc gcgggctccg
     2221 agcccgaacc cgggcccggg gagtcgcgcg gccgcgggtc ggggaggcgg cgggccgtgg
     2281 ccgg
//
LOCUS       DASSWR010000247         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_461979,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000247 DASSWR010000000
VERSION     DASSWR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461979"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFO47_04210"
     CDS             <1..301
                     /locus_tag="VFO47_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET8787931.1"
                     /translation="RVADRAGVRNLGLLCDLYHLATNGDDLDRAIATYGDRVAHVQIA
                     DAPGRHEPGTGSLDLDRHLGALAAGGYDGWVGLEYKPSGASADSFDWLPRERRGA"
     gene            320..1201
                     /locus_tag="VFO47_04215"
     CDS             320..1201
                     /locus_tag="VFO47_04215"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET8787932.1"
                     /translation="MPATVGFIGLGIMGGPMAANLVKAGFDVVGHNRSPEPVRRLVEQ
                     GGRGADGLADAVRDADVVITMVPDSPDVEAVALGEDGIYAHAKPGTLHVDMSSIRPDV
                     ARRVAEAGRERGMRVLDAPVSGGEQGAIDATLSIMVGGEAADFADARPVLEAVGKTVV
                     HVGPAGSGQTVKAANQLIVAGTIELVAEAIVFLEAYGVDTEAAVKVLAGGLAGNAIFD
                     RKGAAMLRRDFTPGFRIELHDKDMGIVTSAAREAGVVIPLGAVVAQLVASLKAQGDGG
                     LDHSALLKLVERLSGRG"
     gene            1229..3028
                     /gene="gcl"
                     /locus_tag="VFO47_04220"
     CDS             1229..3028
                     /gene="gcl"
                     /locus_tag="VFO47_04220"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HET8787933.1"
                     /translation="MPRMRAIAAAVQILQREGVTHAFGVPGAAINPFYAALRDSGAIQ
                     HVLARHVEGAAHMAEGYTRARAGNIGVCVGTSGPAGTDMITGLYSAAADSIPILCITG
                     QAPTARLHKEDFQAVDIAAIAKPVTKLATTVLEPAQVPGAFQQAFQLMRSGRPGPVLL
                     DLPFDVQMAEISFDVDGYEPLPVPRPAATRVQVDRALDLLEAGERPLIVAGGGIINAD
                     AADLLVEFAELVGVPVIPTLMGWGAIPDDHRLMAGMAGLQTAQRYGNANLLASDVVLG
                     IGNRWANRHTGGLDTYTRGRRFVHVDIEPTQIGRVFAPDLGIVSDARAALELFVEAAR
                     ERRDAGRLPDRSGWVAECQERKRTMLRRTHFDDVPVKPQRVYEEMNRAFGKDTRYVST
                     IGLSQIAAGQFLHVYRPRHWINAGQAGPLGWTLPAALGVCVAEPDATVVALSGDYDFQ
                     FMIEELAVGAQFNLPYLHVLVNNSYLGLIRQAQRAFDIDYCVQLAFDNVNSPEVGGYG
                     VDHVKVAEGLGCKALRVREPDEIGPAFEEARKLMAEFRVPVVVEIILERVTNISMGTE
                     IDNVVEFEELALRGEDAPTALGALDLDEMQFLD"
     gene            3028..>3988
                     /locus_tag="VFO47_04225"
     CDS             3028..>3988
                     /locus_tag="VFO47_04225"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET8787934.1"
                     /translation="MRVVVAPDKFKGSLTAAQVAARVAAGLARAAPGAEVLEMPVADG
                     GDGTLEAAVEAGYRRVPVRAEGPTGEPVETAYAERDGVAVVELADVSGLGRLPGGRLA
                     ARTASSYGTGQVIRAALDAGCRRVVLGIGGSAGTDGGAGLVQALGGRLAGEHGGEVGR
                     GGAALAAVRSLDLAGLHPALATAEVVVASDVDNPLLGPRGAAAVYGPQKGASPADVAE
                     LDAALARWADAVRRATGRDEAATPGAGAAGGVGFAVLAVLGATLAPGIDLVLDLVGFR
                     AVLPGAGLVITGEGSLDGQTLHGKAPAGVAAAARASGIPVIAVA"
BASE COUNT          509 a         1535 c         1450 g          494 t
ORIGIN      
        1 acgcgtcgcc gaccgggccg gcgtgcgcaa cctcgggctc ctctgcgacc tctaccacct
       61 ggccaccaac ggcgacgacc tcgaccgggc gatcgccacc tacggcgacc gggtcgccca
      121 cgtgcagatc gccgacgcgc ccgggcgcca tgagccgggc acgggctcgc tcgacctcga
      181 ccgccacctc ggcgccctcg ccgccggcgg ctacgacggc tgggtcgggc tggagtacaa
      241 gcccagcggg gccagcgccg acagcttcga ctggctgccc cgcgagcgcc gcggcgcctg
      301 accttcaagg agacacgcga tgccagcaac cgtcggcttc atcggcctcg gcatcatggg
      361 cggcccgatg gccgccaacc tcgtcaaggc cggcttcgac gtcgtcggcc acaaccgctc
      421 ccccgagccg gtccggcggc tcgtcgaaca ggggggccgc ggggccgacg ggctcgccga
      481 cgccgtccgc gacgccgacg tggtcatcac catggtcccc gactcgcccg acgtggaggc
      541 ggtggcgctg ggggaggacg gcatctacgc ccacgccaag ccgggcaccc tgcacgtcga
      601 catgagctcg atccgccccg acgtggccag gagggtcgcc gaggccggcc gggagcgggg
      661 catgcgcgtc ctcgacgccc cggtgagcgg gggcgagcag ggggccatcg acgccaccct
      721 gtccatcatg gtcggcgggg aggccgccga cttcgccgac gcccggcccg tcctcgaggc
      781 ggtcggcaag acggtggtcc acgtcggacc ggccgggtcc ggccagaccg tcaaggccgc
      841 caaccagctg atcgtggccg gcaccatcga gctggtggcc gaggccatcg tcttcctgga
      901 ggcctacggg gtcgacaccg aggccgcggt caaggtgctg gccggtggcc tggccggcaa
      961 cgccatcttc gaccgcaagg gcgcggccat gctgcgccgc gacttcaccc ccgggttccg
     1021 gatcgagctg cacgacaagg acatggggat cgtcacctcg gccgcccgcg aggccggcgt
     1081 ggtcatcccc ctcggcgccg tcgtggccca gctggtcgcc agcctcaagg cccagggcga
     1141 cggcggcctt gaccactcgg cgctgctcaa gctggtcgag cggctgtcgg gccgcggctg
     1201 accgtcaccg ggtacccagg agctcgccat gccccgaatg agagcgatcg ccgccgccgt
     1261 ccagatcctg cagcgcgaag gcgtgaccca cgccttcggg gtgccgggcg cggccatcaa
     1321 ccccttctac gccgccctgc gggacagcgg cgccatccag cacgtgctgg cccgccacgt
     1381 cgagggcgcg gcccacatgg ccgagggcta cacccgggcc cgggccggca acatcggcgt
     1441 ctgcgtcggc acctcgggcc cggccggcac cgacatgatc accggcctgt actcggcggc
     1501 cgccgactcc atcccgatcc tctgcatcac cgggcaggct cccacggccc ggctgcacaa
     1561 ggaggacttc caggccgtcg acatcgccgc catcgccaag ccggtgacca agctggcgac
     1621 cacggtgctg gagccggccc aggtgccggg cgcgttccag caggcgttcc agctgatgcg
     1681 ctccggccgt cccgggccgg tcctgctcga cctgcccttc gacgtgcaga tggccgagat
     1741 ctccttcgac gtcgacggct acgagccgct cccggtcccc cggccggcgg ccacccgggt
     1801 ccaggtcgac agggcccttg acctgctcga ggcgggcgag cggccgctga tcgtggccgg
     1861 cggcgggatc atcaacgccg acgccgccga cctgctggtg gagttcgccg agctggtcgg
     1921 cgtcccggtg atcccgacgc tgatgggctg gggggccatc cccgacgacc accggctgat
     1981 ggccggcatg gccgggctgc agaccgccca gcgctacggc aacgccaacc tgctcgcctc
     2041 cgacgtcgtg ctcggcatcg gcaaccgctg ggccaaccgc cacaccggcg ggctcgacac
     2101 ctacacccgc ggccgccgct tcgtccacgt cgacatcgag cccacccaga tcggccgcgt
     2161 cttcgccccc gacctcggca tcgtctcgga cgcacgcgcg gccctcgagc tgttcgtgga
     2221 ggcggcccgg gagcgccgcg acgccgggcg gctgccggac cggtcgggct gggtcgccga
     2281 gtgccaggag cgcaagcgga ccatgctgcg gcgcacccac ttcgacgacg tgccggtgaa
     2341 gccgcagcgg gtctacgagg agatgaaccg ggccttcggc aaggacaccc gctacgtcag
     2401 caccatcggc ctgtcgcaga tcgccgccgg gcagttcctc cacgtctacc ggccccgcca
     2461 ctggatcaac gccggccagg cgggcccgct gggctggacc ctgccggccg ccctgggcgt
     2521 gtgcgtggcc gagcccgacg ccaccgtggt cgccctgtcg ggcgactacg acttccagtt
     2581 catgatcgag gagctggccg tcggggccca gttcaacctg ccctacctcc acgtgctggt
     2641 gaacaactcc tacctggggc tgatccgcca ggcccaacgg gccttcgaca tcgactactg
     2701 cgtgcagctg gccttcgaca acgtcaactc cccggaggtc ggcggctacg gggtcgacca
     2761 cgtcaaggtg gccgagggcc tcggctgcaa ggccctgcgg gtgcgcgagc cggacgagat
     2821 cggcccggcc ttcgaggagg ccaggaagct catggccgag ttccgggtgc cggtcgtggt
     2881 cgagatcatc ctggagcggg tgaccaacat ctccatgggg accgagatcg acaacgtcgt
     2941 cgagttcgag gagctggccc tgcgcggcga ggacgccccc accgccctcg gcgccctcga
     3001 cctcgacgag atgcagttcc tcgactgatg cgcgtcgtcg tcgccccgga caagttcaag
     3061 ggctccctga ccgcggccca ggtggccgcc cgggtcgccg ccgggctggc ccgcgccgcc
     3121 cccggcgccg aggtgctcga gatgccggtc gccgacggcg gcgacgggac cctggaggcg
     3181 gccgtcgagg ccggctaccg ccgcgtcccc gtccgggccg aggggcccac cggcgagccg
     3241 gtcgagaccg cctacgccga gcgcgacggc gtggccgtgg tcgagctggc cgacgtgtcc
     3301 gggctgggcc ggcttcccgg cgggcgcctg gccgcccgga ccgcctccag ctacggcacc
     3361 ggccaggtga tccgggccgc cctggacgcc ggctgccgcc gggtcgtcct cggcatcggg
     3421 ggcagcgccg gcaccgacgg cggcgccggc ctggtccagg cgttgggcgg gcgcctggcc
     3481 ggagagcacg gcggggaggt cgggcgcggc ggggcggcgc tggccgcggt ccgctcgctc
     3541 gacctggccg gcctgcaccc ggccctggcc accgccgagg tcgtggtggc cagcgacgtc
     3601 gacaaccccc tgctcggccc tcggggggcg gcggccgtgt acggccccca gaagggggcg
     3661 tccccggccg acgtggccga gctggacgcc gccctggccc gctgggccga cgccgtccgc
     3721 cgggcaaccg gccgggacga ggccgcgacc ccgggcgcgg gcgcggccgg cggcgtcggg
     3781 ttcgccgtcc tggccgtgct cggcgcgacc ctggccccgg gcatcgacct ggtcctcgac
     3841 ctggtcggct tccgtgccgt gctgccgggg gcggggctgg tgatcacggg ggaggggtcg
     3901 ctcgacggcc agaccctgca cggcaaggcg ccggccgggg tggccgcggc cgcccgcgcc
     3961 tccgggatcc cggtgatcgc ggtggccg
//
LOCUS       DASSWR010000248         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_715110,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000248 DASSWR010000000
VERSION     DASSWR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715110"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>2319
                     /locus_tag="VFO47_04230"
     CDS             32..>2319
                     /locus_tag="VFO47_04230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET8787935.1"
                     /translation="MTAALMRAGKAVRVSEDEGDGELTRRLREEGWRDCLAAPVRVEG
                     KVIAVLCTYNRTGFEGFEEGELAVLDALAAEVGTAIEKGELLEAILEERTKLSEIVDN
                     TSDGIAALDPDGTVSSWNPGFEEITGYRSADMVGSRGLARLRPRDVADREVQLDRWAE
                     EPGTLPTVVEVLRPNGEACWMACSWTRVPAVDGRPRRLILTSRDITKELELKRAEKAL
                     RDSAARFRALVQNSSHMVIVLDAGGGITYASPAFRRMLGYRDDARVGQNVFELIHPDD
                     VGDVRARFGEHLAGLAQGAGLGFRFLAADGSWRNIEALANNLLDDPAVGGVVFNCRDV
                     TERTRAEGQLAGQAQVLDLIARDAPLMETLDTLAKVIEAEASGARCAILLLDDSSEAL
                     TVAAAPSLIDVGLHEADGLVIGPEAGTSGTAAFRREPVISADVASDPLWRESRQVALA
                     RGIRAAWSTPIIAADGDPVLGVLTVYFDEPRRPGPAERSLLELAAHLADIAIERSHAQ
                     AQLAHQAAHDALTGLPNRVFFLDRITLALARTQRSRSSVAVLFLDLDRFKFINDSLGH
                     DAGDRLLVALGKRLQDVIRPGDTVARFGGDEFTILCESIADEAHALAIAERVAQVATA
                     PFPLGDAEVFVTMSIGIALASGSRVRPESLVENADAAMYRAKARGGNRREMFDQAMRA
                     RAKRRLAMHRSRHRAVERGEFRVLYQPIHCLGSGRAVGAEALVRWEHPDRGLIGPGEF
                     VALAEETGLIMSIGTQVLRQACR"
BASE COUNT          310 a          880 c          834 g          295 t
ORIGIN      
        1 gcagcgggtc gaggaggccg gcagggagac cgtgaccgcg gccctgatgc gcgccggcaa
       61 ggcggtccgg gtcagcgagg acgagggcga cggcgagctg acccgccggc tgcgcgagga
      121 gggctggcga gactgcctgg ccgccccggt gcgggtcgag ggcaaggtca tcgcggtcct
      181 gtgcacctac aaccgcaccg gcttcgaggg cttcgaggag ggcgagctgg ccgtcctgga
      241 cgccctggcg gccgaggtgg gcacggccat cgagaagggc gagctgctcg aggccatcct
      301 cgaggagcgc accaagctct ccgagatcgt cgacaacacc tccgacggca tcgccgccct
      361 cgaccccgac gggaccgtgt ccagctggaa cccggggttc gaggagatca ccggctaccg
      421 gtcggccgac atggtcggct cccgcggcct ggcccggctg cggccccgcg acgtcgccga
      481 ccgcgaggtc cagctggacc gctgggccga ggagccgggg acgctgccaa ccgtggtcga
      541 ggtgctgcga cccaacggcg aggcgtgctg gatggcctgc tcctggaccc gggtcccggc
      601 ggtcgacggc cggccgcgcc ggctcatcct cacctcccgg gacatcacca aggagctgga
      661 gctcaagcgg gccgagaagg ccctgcggga ctcggccgcc cgcttccggg cgctggtcca
      721 gaactcctcc cacatggtga tcgtgctcga cgccggcggc ggcatcacct atgccagccc
      781 cgccttccgg cggatgctcg gctaccgcga cgacgcccgg gtcgggcaga acgtgttcga
      841 gctgatccac cccgacgacg tcggcgacgt ccgggcccgc ttcggcgagc acctggccgg
      901 cctggcccag ggcgccgggt tggggttccg cttcctggcc gccgacggtt cctggcgcaa
      961 catcgaggcc ctggccaaca acctgctcga cgacccggcc gtcggcgggg tggtgttcaa
     1021 ctgccgcgac gtgaccgagc gcacccgggc cgagggccag ctggccggtc aggcccaggt
     1081 gctggacctg atcgcccggg acgcgcccct gatggagacc ctcgacaccc tggccaaggt
     1141 gatcgaggcc gaggcgtcgg gcgcccgctg cgccatcctg ttgctggacg acagctccga
     1201 ggcgttgacc gtggccgccg ccccctcgct catcgacgtc ggcctccacg aggccgacgg
     1261 gctggtcatc gggcccgagg ccgggaccag cggcacggcc gcgttccggc gcgagccggt
     1321 gatctccgcc gacgtggcca gcgacccgct gtggcgcgag tcacgccagg tcgcgctggc
     1381 cagagggatc cgggccgcct ggtcgacgcc gatcatcgcc gccgacggcg accctgtcct
     1441 cggcgtcctc accgtctact tcgacgagcc ccgccgtccc gggccggccg agcggagcct
     1501 gctggagctg gccgcccacc tggccgacat cgccatcgag cgcagccacg cccaggccca
     1561 gctggcccac caggcggccc acgacgccct caccggcctg cccaaccggg tcttcttcct
     1621 ggacaggatc accctggccc tggcccgcac ccagcgcagc cgctcctcgg tggccgtgct
     1681 cttcctcgac ctggaccgct tcaagttcat caacgactcg ctcggccacg acgccggcga
     1741 ccggctgctg gtcgccctgg gcaagcgcct ccaggacgtc atccggcccg gcgacaccgt
     1801 ggcccgcttc ggcggcgacg agttcaccat cctctgcgag agcatcgccg acgaggccca
     1861 cgccctggcc atcgccgagc gggtcgcgca ggtggccacc gccccgttcc cgctcggcga
     1921 cgccgaggtg ttcgtcacca tgagcatcgg catcgcgctg gcctcgggca gccgggtccg
     1981 gcccgagtcg ctggtcgaga acgccgacgc cgccatgtac cgggccaagg cgcggggcgg
     2041 caaccggcgc gagatgttcg accaggcgat gcgggccagg gccaagcgcc gcctggccat
     2101 gcaccgctcg cggcaccggg cggtcgagcg cggcgagttc cgggtcctct accagccgat
     2161 ccactgcctg ggcagcggcc gcgcggtggg cgcggaggcg ctggtgcgct gggagcatcc
     2221 cgaccggggc ctgatcggcc cgggggagtt cgtcgccctg gccgaggaga ccggcctgat
     2281 catgtccatc ggcacccagg tgctgcgcca ggcctgccg
//
LOCUS       DASSWR010000249         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1195899, whole genome shotgun sequence.
ACCESSION   DASSWR010000249 DASSWR010000000
VERSION     DASSWR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195899"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFO47_04235"
     CDS             complement(<1..554)
                     /locus_tag="VFO47_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET8787936.1"
                     /translation="MRVVVVGGAGAMGRWTVRDLTESEGVDEVVVADLDGARAREAAG
                     WAAARSGSNGTAQVSGLALDAGDPEALRRAFDGADVVCNCAVYALNLPVMDACLDAGT
                     HYVDLGGLFHTTRRQLALHDRFVAAGVTAVVGMGGSPGITNVLAALAARGLDIVEEVE
                     VRLGVADFAPSSAPVPVPYAIQTIL"
     gene            666..2261
                     /locus_tag="VFO47_04240"
     CDS             666..2261
                     /locus_tag="VFO47_04240"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET8787937.1"
                     /translation="MATTVADLLAIDELRLGLVAGAKGLERPIRWAHVSELRDPTRFL
                     RGGEVLLTTGLGLRGAPAGQAAYVEQLARADLAALGIGLGFAFKATPPAVLEAADRAG
                     FPVFEVPFEVPFIAITEALFSRLVNEQYVLLQRAGTVQQTLSRLLVEGAGLDALLEAY
                     ARMTGTRALLLDERGEVMAAARGATAVLPPRAVWAEIQALRPEGNEFSLSLSDEGGSR
                     TLLPVLVGGGPAGFLVLHRGARPEPFHQVVVHHLATAVALELAKAQAVARTERRLVGD
                     FLDALLEGELSASEIRRRLRFLGLGASPAIAVLVARREEPDGAEAAVEALRLLVQDRL
                     SRRPAPYVLSVHDASAVALFEAADAAEARAAADAVAEAVLARGLGARFGLGMPEADPR
                     GLRRAYQEARFALEAAGPPTGTGEPRVATVADLGSHRLLLARQEDAALEAISRGLLDP
                     LADYDRRHHGDLVASLRAFLEHNGNWEAAARALAVHRHTLRYRIRRIAELTGRDLDRA
                     GDRVEFWLALQAADVLHGRGFGD"
     gene            2403..3584
                     /locus_tag="VFO47_04245"
     CDS             2403..3584
                     /locus_tag="VFO47_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787938.1"
                     /translation="MADTKVQAGARRAVPEEDGLAAIELVGVEKEFTAGGHDVRAVER
                     VDLRIAEGEFFSLLGPSGCGKTTTLRMIAGFEEPTSGQILLHGRDMVGVPPFRRDVNM
                     VFQQYALFPHMDVFENVAFGLRRKKVDKDEIKRRVAEALALVELEGREKRKPRQLSGG
                     QQQRVALARALVNRPRALLLDEPLGALDLKLRQAMQLELKRIQREVGITFVYVTHDQE
                     EALTMSDRLVVMNAGRIEQLGSPRELYEHPATRFVANFIGTSNILTGRLERRGDAWAL
                     AGLGPDQRVLVADNDDAREGQEVELAIRPEKIVLRTEQDPPSPDRTALKVKVDEVVYL
                     GTSTQYRTVTDGGEAIAVYRQNASATPGADVLAGQVGWLEWPAEHSYVLGGGTKEEAL
                     P"
     gene            3581..>4205
                     /locus_tag="VFO47_04250"
     CDS             3581..>4205
                     /locus_tag="VFO47_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8787939.1"
                     /translation="MTRNRPLDPAWVRGMTMPRISRRDALRGAGLLSASAFLAACGVE
                     GSGGGDEAPQASGFWASQTKAGVLNFANWPLYMDQEKQGGKTVYPSLQDFTKETGIKV
                     TYKEVIEDNDSFLGKITPSLQAGQDTGWDLIVITNGGPIEKLIRQNFLIELDHSKLPT
                     FAKNAAQSVKNPSYDPGNKYTVAWQSGLVGLAYNPKLTKREITSWEDL"
BASE COUNT          598 a         1560 c         1505 g          542 t
ORIGIN      
        1 aggatcgtct ggatggcgta cggcacgggc acgggggcgc tggagggggc gaagtcggcc
       61 acgcccaggc ggacctcgac ctcctcaacg atgtccagcc cgcgggcggc cagcgccgcc
      121 agcacgttgg tgatgccggg gctgccgccc atcccgacca cggccgtgac cccggcggcc
      181 acgaaccggt cgtgcagggc cagctgccgg cgggtggtgt ggaacaggcc gcccaggtcg
      241 acgtagtggg tcccggcgtc gaggcaggcg tccatcaccg gcaggttgag ggcgtacacg
      301 gcgcagttgc agaccacgtc ggcgccgtcg aaggcccgcc gcagcgcctc ggggtcgccg
      361 gcgtccaggg cgaggccgct gacctgggcg gtcccgttgc tcccggacct ggccgccgcc
      421 cagccggccg cctcccgggc ccgcgccccg tccaggtcgg ccaccacgac ctcgtcgacg
      481 ccctcggact cggtcaggtc gcgcaccgtc cagcgcccca tggcgccggc gccaccgacc
      541 acgacgactc gcatcaacac ctccggatag acccgcaggc tgtcagccgg gcaagtccac
      601 gcggtagtgc cctcccgtcc agcttccggg tacgctgttg tacgtttcgg cgtagaaggg
      661 ggaccgtggc caccaccgtt gccgacctgc tggcgatcga cgagctccgc ctcggcctgg
      721 tcgccggggc caaggggctg gagcggccga tccgctgggc gcacgtgtcc gagctgcggg
      781 acccgacccg gttcctgcgc ggcggcgaag tgctgctcac caccgggctg gggctgcgcg
      841 gcgcccccgc cggccaggcc gcctacgtcg agcagctggc cagggccgac ctggccgccc
      901 tcgggatcgg gctcgggttc gccttcaagg ccaccccgcc ggccgtgctc gaggcggccg
      961 accgggccgg cttcccggtg ttcgaggtcc ccttcgaggt gccgttcatc gccatcaccg
     1021 aggccctgtt ctcccgcctc gtcaacgagc agtacgtcct gctccagcgg gccgggacgg
     1081 tccagcagac gctgtcgcgg ctgctggtcg agggggccgg gctggacgcc ctgctggagg
     1141 cctacgcccg gatgaccggg accagggcgc tgctgctgga cgagcgcggc gaggtgatgg
     1201 cggcggcccg gggcgccacc gccgtgctcc cgccccgcgc cgtctgggcc gagatccagg
     1261 ccctgcggcc cgaaggcaac gagttctccc tcagcctgtc ggacgagggc ggcagccgca
     1321 ccctgctgcc cgtcctggtc gggggcggcc cggccgggtt cctggtgctg caccggggcg
     1381 cccgccccga gcccttccac caggtggtcg tccaccacct ggccaccgcc gtcgccctgg
     1441 agctggccaa ggcccaggcg gtggccagga ccgagcgccg gctggtcggc gacttcctcg
     1501 acgccctgct cgagggggag ctgtcggcct ccgagatccg ccggcgtctc cgcttcctcg
     1561 gcctcggcgc ctccccggcg atcgcggtgc tggtggccag gcgggaggag cccgacggcg
     1621 ccgaggcggc cgtggaggcg ctgcggctgc tcgtccagga ccgcctctcg cgccgcccgg
     1681 cgccgtacgt gctcagcgtc catgacgcct cggcggtcgc cctgttcgag gcggccgacg
     1741 cggccgaggc ccgcgcggcc gccgacgcgg tggccgaggc ggtcctggcc agggggctgg
     1801 gcgcccgctt cggcctcggc atgcccgagg ccgacccgcg cggcctgcgc cgcgcctacc
     1861 aggaggcccg gttcgccctt gaggcggccg gcccgcccac cgggaccggc gagccgcggg
     1921 tcgcgaccgt tgccgacctc ggcagccacc gcctgctgct ggccaggcag gaggacgccg
     1981 ccctggaggc catctcccgc ggcctgctcg accccctggc cgactacgac cggcgccacc
     2041 acggcgacct ggtggccagc ctgcgggcct tcctcgagca caacggcaac tgggaggcgg
     2101 cggccagggc gctggccgtg caccgccaca ccctgcgcta ccggatccgc cggatcgccg
     2161 agctcaccgg ccgcgacctg gaccgggccg gcgaccgggt cgagttctgg ctcgccctcc
     2221 aggccgccga cgtgctccat ggccggggct tcggggactg atacctacgg tgacgaattg
     2281 gcagccagga gccccgcgcg gtgccggttc gtacatggac gccccggggc ggaatgctag
     2341 cgtccatatg ttcccgtgtg gtcgaatcgg cggatcggcc gcctgagacg agggacgagg
     2401 cgatggcaga cacgaaggtc caggcgggcg cccgccgggc cgtccctgaa gaggacggcc
     2461 tggccgcgat cgagctggtc ggggtggaga aggagttcac cgccgggggc cacgacgtcc
     2521 gggctgtcga gcgggttgac ctcaggatcg ccgagggcga gttcttctca ctgctgggcc
     2581 cctcgggctg cggcaagacg accaccctgc ggatgatcgc cggcttcgag gagccgacca
     2641 gcggccagat cctcctccac ggccgcgaca tggtcggcgt cccgccgttc cggcgcgacg
     2701 tcaacatggt cttccagcag tacgccctct tcccccacat ggacgtcttc gagaacgtgg
     2761 ccttcggcct gcggcgcaag aaggtcgaca aggacgagat caagcggcgg gtggccgagg
     2821 cgctggccct ggtcgagctg gagggccgcg agaagcgcaa gccgcgccag ctgtcgggcg
     2881 gccagcagca gcgggtcgcc ctggccaggg ccctggtcaa ccggccacgg gccctgctgc
     2941 tggacgagcc gcttggcgcc ctcgacctca agctgcgcca ggcgatgcag ctggagctga
     3001 aacggattca gcgggaggtg gggattacct tcgtgtacgt cacccacgac caggaggagg
     3061 ccctgaccat gtccgaccgg ctggtggtga tgaacgccgg ccggatcgag cagctcggca
     3121 gcccgcgcga gctgtacgag caccccgcca cccggttcgt ggccaacttc atcgggacct
     3181 cgaacatcct caccggccgg ctggagcgca ggggcgacgc ctgggcgctg gccgggctgg
     3241 gcccggacca acgggtgctg gtcgccgaca acgacgacgc ccgtgagggc caggaggtcg
     3301 agctggccat ccggcccgag aagatcgtgc tgcggaccga gcaggaccca ccctcgcccg
     3361 accgcaccgc cctcaaggtc aaggtcgacg aggtcgtcta cctcggcacc tcgacccagt
     3421 accgcacggt gaccgatggc ggcgaggcca tcgccgtgta ccgtcagaac gcgtccgcca
     3481 cccccggagc ggacgtgctc gccggccagg tgggatggct ggaatggccc gcggagcact
     3541 cctatgtgct cggtggcggc accaaggagg aagctctgcc gtgaccagga accgaccgct
     3601 cgaccccgcc tgggtgcggg gaatgaccat gccccggatc agccgccgcg acgccctccg
     3661 aggggccggc ctgctcagcg cctcggcctt cctggccgcc tgcggcgtgg aggggtcggg
     3721 cggcggcgac gaggcgcccc aggcctcggg gttctgggcc agccagacca aggccggcgt
     3781 cctcaacttc gccaactggc ccctgtacat ggaccaggag aagcagggcg gcaagaccgt
     3841 ctacccctcg ctccaggact tcaccaagga gacggggatc aaggtcacct acaaagaggt
     3901 catcgaggac aacgactcct tcctcggcaa gatcaccccc tcgctccagg ccggccagga
     3961 caccggctgg gacctgatcg tgatcaccaa cggcgggccg atcgagaagc tcatccgcca
     4021 gaacttcctg atcgagctcg accactccaa gctgcccacg ttcgccaaga acgccgccca
     4081 gtcggtcaag aaccccagct acgacccggg caacaagtac accgtggcct ggcagtcggg
     4141 gctggtcggg ctggcctaca accccaagct gaccaagcgg gagatcacca gctgggagga
     4201 cctca
//
LOCUS       DASSWR010000250         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_715142,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000250 DASSWR010000000
VERSION     DASSWR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715142"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1421
                     /locus_tag="VFO47_04255"
     CDS             108..1421
                     /locus_tag="VFO47_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET8787940.1"
                     /translation="MSSPRRRRALATLALALALALAAAACGDSSGDGDQSSAQSGPRT
                     LTIWLMDASAPPAVIDAVNAEWKKAHPNDTVKVQLQQWNNITTKLDAAFAGSSPPDVV
                     ELGNTLVSKYAAAGALQDLTGNRGEFENNQAWLQSLTESCTMDGKLVCAPYLAGSRAV
                     IYRKSMFAKAGITETPTSLEELQTAAEKLMSTFGDNKRFSAFYFPGKYWYAALPFVWD
                     HGGDVAVQDGGQWRGTLDSAESQSGLTALKQLVDCCSRADKQGDELKQDQAFAQGHIA
                     MIVANGWEKGVILDKKEGNPKLESDLAAFPLPSRTSGQTAPVFLGGSDLGIAAKSKSQ
                     DLALDWVKLLAGTKFQTQMATVGGVIPNSTTLLDLHADDPYLSVFDATAKNSRFTPTS
                     PNWANVEAANVLPDMLVSIFTERASIADATKKASSQIADVLNSGS"
     gene            1399..2367
                     /locus_tag="VFO47_04260"
     CDS             1399..2367
                     /locus_tag="VFO47_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8787941.1"
                     /translation="MSSIQDRRTFTEARPATDLAGRAAQRRRRLAPLVPYALLAPALL
                     VLAAILGFPMYRLVSLSFQRYGLRELFAGQGTWIGLENYRDIVTDSFFWTVVARTMLF
                     AAVAVGLTMVLGTLVALLLRNLGRRMRVAVTAGLVAAWAMPTLTAISIWQWLFDFEFG
                     IVNWLLTWLGVDGFEQHNWFDQPLQGFTVIMIVVVWGALPFVAITLHAGLTQVPRELE
                     EAARVDGAGAFQVFRHVTLPVMKPIFLILATLSTIWDFKVFTQIWVLLNQRPSREYFL
                     LGIWSYSESFGVTRYGKGAAIAVVMVLLLLIVTSFYVRQMVRAVGP"
     gene            2364..3194
                     /locus_tag="VFO47_04265"
     CDS             2364..3194
                     /locus_tag="VFO47_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8787942.1"
                     /translation="MTRRRLTRVGYNAAAVLVLAVTVFPIYWMVATGFKRGPDILSFE
                     PRWLPIPGTLENYRSAVAKPHFLQDVRNSMLVVVATVLISLVLAFLAALAAGRFNFRG
                     RAAMIVMLIVVQMVPLEALIIPIYLMLDGAGQTDRLAGIVVTYLTFVLPFSIWTLRGF
                     IVNIPTELEEAAMVDGCTRAGAFRRILFPLVAPGLVATAVFSFILVWNDYLIAYVLLR
                     SPEKQTLGIWLASFTTNHGTEWGGVMAGSTLFAIPAVVFFLLVQRRVVSGLTAGAVKG
                     "
     gene            3191..>3601
                     /locus_tag="VFO47_04270"
     CDS             3191..>3601
                     /locus_tag="VFO47_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787943.1"
                     /translation="MSIGTSYFGNRILRHVAADMEGLAADGFTGVLHTYTENDLAYYR
                     EAMGRIVAASHAAGLQVQVAPWGVAQMFGGEAESRFTAFNPDAGQVLDDGRATPAGCP
                     NNPAVRAFVRSWADAAIDTGADRVFWDEPHWVHPE"
BASE COUNT          516 a         1351 c         1163 g          571 t
ORIGIN      
        1 ccaggtggtg gtgaccgtgg ccgagggcgc cgtcgtccac ggcggcgacc gcctgccccg
       61 cccatcacaa ccagccatcg gccgttccag ctcaaggagg taggcccatg tccagcccgc
      121 gccgccggcg cgccctcgcc accctcgccc tcgccctcgc cctggccctc gcggcggcgg
      181 cctgcggaga ttcctccggc gacggcgacc agtccagcgc ccagtccggg ccccgcacgc
      241 tcaccatctg gctgatggac gcgagcgccc cgccggcagt catcgacgcc gtcaacgctg
      301 agtggaagaa ggcccacccg aacgacaccg tcaaggtcca gctccagcag tggaacaaca
      361 tcacgaccaa gctcgacgcc gccttcgccg gctccagccc gccggacgtg gtcgagctcg
      421 gcaacaccct ggtctccaag tacgccgccg ccggggcgct ccaggacctg accggcaacc
      481 gcggcgagtt cgagaacaac caggcctggc tccagtcgct caccgagtcc tgcaccatgg
      541 acggcaagct cgtctgcgcc ccctacctgg ccggcagccg ggccgtcatc taccgcaaga
      601 gcatgttcgc caaggccggc atcaccgaga ccccgaccag cctggaggag ctccagacgg
      661 ccgccgagaa gctgatgtcg accttcggcg acaacaagcg gttctcggcc ttctacttcc
      721 ccggcaagta ctggtacgcg gcgctcccgt tcgtctggga ccacggcggc gacgtggccg
      781 tgcaggacgg cggccagtgg cgcggcaccc tcgacagcgc cgagtcccag tccggcctga
      841 ccgccctcaa gcagctggtc gactgctgct cgcgggctga caagcagggt gacgagctca
      901 agcaggacca ggccttcgcc cagggccaca tcgccatgat cgtggccaac ggctgggaga
      961 agggggtcat cctcgacaag aaggagggca accccaagct ggagagcgac ctggccgcct
     1021 tcccgctgcc cagccgcacc agcggccaga ccgcgccggt gttcctcggc ggctccgacc
     1081 tgggcatcgc cgccaagtcc aagagccagg acctggccct tgactgggtg aagctgctgg
     1141 ccgggaccaa gttccagacc cagatggcca ccgtgggcgg ggtcatcccc aactccacca
     1201 ccctgctcga cctgcacgcc gacgacccct acctgtcggt gttcgacgcc accgccaaga
     1261 acagccgctt caccccgacc tcgcccaact gggccaacgt cgaggcggcc aacgtcctgc
     1321 ccgacatgct ggtctcgatc ttcaccgagc gggcgtcgat cgccgacgcc accaagaagg
     1381 cgagcagcca gatcgccgat gtcctcaatt caggatcgta ggaccttcac ggaggcgcgc
     1441 ccggcgaccg acctcgccgg gcgcgccgcc caacggcggc gccggctggc cccgctggtg
     1501 ccctacgccc tgctcgcccc ggccctgctg gtgctggcgg cgatcctcgg gttcccgatg
     1561 taccggctgg tctcgctctc gttccagcgc tacgggctcc gggagctgtt cgccggccag
     1621 ggcacctgga tcggcctgga gaactaccgc gacatcgtca ccgactcgtt cttctggacg
     1681 gtggtggcca ggacgatgct gttcgcggcc gtcgccgtcg gcctgaccat ggtcctcggc
     1741 accctggtgg ccctgctcct gcgcaacctg ggccggcgga tgcgggtggc ggtcacggcc
     1801 gggctggtgg ccgcctgggc gatgccgacc ctcaccgcca tctccatctg gcagtggctg
     1861 ttcgacttcg agttcgggat tgtcaactgg ctgctcacct ggctcggggt cgacgggttc
     1921 gagcagcaca actggttcga ccagcccctc caggggttca cggtgatcat gatcgtggtc
     1981 gtctgggggg cgctgccgtt cgtggccatc accctgcacg ccggcctgac ccaggtcccg
     2041 cgcgagctcg aggaggcggc ccgggtcgac ggcgccggcg ccttccaggt gttccggcac
     2101 gtcaccctcc cggtgatgaa gccgatcttc ctgatcctgg ccaccctctc caccatctgg
     2161 gacttcaagg tcttcaccca gatctgggtg ctgctcaacc agcgccccag ccgcgagtac
     2221 ttcctgctcg gcatctggtc ctacagcgag tcgttcgggg tgacccgcta cggcaagggg
     2281 gcggccatcg ccgtcgtcat ggtcctgctg ctgctgatcg tgacctcgtt ctacgtccgg
     2341 cagatggtgc gggcggtggg cccgtgaccc gccgccgcct gaccagggtc ggctacaacg
     2401 ccgccgccgt gctggtactg gccgtcaccg tgttcccgat ctactggatg gtggcgacgg
     2461 gcttcaagcg gggtcccgac atcctgtcgt tcgagcccag gtggctgccg atcccgggga
     2521 cgctggagaa ctaccgctcg gccgtcgcca agccccactt cctccaggac gtccgcaact
     2581 cgatgctggt ggtcgtggcc acggtgctga tctcgctcgt cctggccttc ctggccgcgc
     2641 tggccgccgg ccggttcaac ttccgcggcc gggcggccat gatcgtcatg ctgatcgtgg
     2701 tccagatggt gccgctggag gccctcatca tccccatcta cctgatgctg gacggggccg
     2761 gccagaccga ccggctggcc gggatcgtcg tcacctacct gaccttcgtg ctgccgttca
     2821 gcatctggac gctgcgcggc ttcatcgtca acatccccac cgagctcgag gaggcggcga
     2881 tggtcgacgg ctgcacccgc gccggggcgt tccggcgcat cctgttcccg ctggtcgccc
     2941 ccgggctggt cgccaccgcg gtgttctcct tcatcctggt ctggaacgac tacctgatcg
     3001 cctacgtgct gctgcgcagc cccgagaagc agaccctcgg gatctggctg gcctccttca
     3061 ccaccaacca cggcaccgag tggggcgggg tgatggccgg gtcgaccctg ttcgccatcc
     3121 cggcggtggt gttcttcctg ctcgtccagc gccgggtggt cagcggcctg acggcgggag
     3181 cggtgaaggg ttgagcatcg gcacctcgta cttcgggaac cgcatcctgc gccacgtcgc
     3241 cgccgacatg gaggggctgg ccgccgacgg gttcaccggg gtgctgcaca cctacaccga
     3301 gaacgacctg gcgtactacc gcgaggccat gggccggatc gtggccgcct cccacgccgc
     3361 cggcctccag gtgcaggtcg ctccctgggg ggtggcccag atgttcggcg gcgaggccga
     3421 gagccgcttc accgccttca accccgacgc cggccaggtc ctggacgacg gccgggcgac
     3481 cccggccggc tgccccaaca acccggccgt gcgggcgttc gtccgctcct gggccgacgc
     3541 ggccatcgac accggcgccg accgcgtctt ctgggacgag ccccactggg tccaccccga
     3601 g
//
LOCUS       DASSWR010000251         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_123061,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000251 DASSWR010000000
VERSION     DASSWR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123061"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFO47_04275"
     CDS             complement(<1..405)
                     /locus_tag="VFO47_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HET8787944.1"
                     /translation="MKLALGAVLTFFAVALAGQRAERITRLIASGQPAPDRFEGAWTR
                     LRAELVEVFVQRKLLKRPIPGLAHFFTFWGFVILLTTIAEAYGELFSETFALPLVGHS
                     PLLGFLQDLTAVAVLVSLAVFAVIRIRQSPARQ"
     gene            490..1092
                     /locus_tag="VFO47_04280"
     CDS             490..1092
                     /locus_tag="VFO47_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET8787945.1"
                     /translation="MRDPTQPQSRPSRARVGLLAGGLLLVVAGVAVLAWVLASSAGLV
                     GRDDPVMADPPATTTPAASGAARTVTYRLEPAGGEEARTVRLEIAATPAARARGLMGR
                     TEVPEGTGMVFLYPEDVAEAYWMKNTLVPLSIAFVAADGRVVSVSEMTPCRADPCPSY
                     APAGPYRYAVELAAGSFAAAGVGPGAKVVPEDPAALPEPA"
     gene            1205..>2233
                     /locus_tag="VFO47_04285"
     CDS             1205..>2233
                     /locus_tag="VFO47_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET8787946.1"
                     /translation="MAKAVGIDLGTTNSVVSTLEGGEPTVIPNAEGARTTPSVVALAK
                     TGEVLVGEVAKRQAVTNPDRTVRSVKRHMGESSWRFRADGKEFSPQQVSAFILQKLKR
                     DAEAYLGDTVTQAVITVPAYFDDAQRQATKEAGEIAGLDVLRIINEPTAASLAYSLDK
                     ENDETILVFDLGGGTFDVSLLDIGEGVIEVQATSGDMHLGGDDWDQRIVEWLLTTFKN
                     NHGIDLSNDKMAMQRLKEAAEKAKIELSSTLETTINLPFITATPEGPLHMEQRLTRGE
                     LERMTSDLVERLRGPFDKAVTDWKAKTQKSLTDIHHVVLVGGSTRMPMVVEFVKQLTG
                     GKEPHKGVN"
BASE COUNT          368 a          793 c          772 g          300 t
ORIGIN      
        1 ctggcgggcg ggcgactggc ggatgcggat gacggcgaag acggccaggc tgaccagcac
       61 ggcgaccgcg gtcaggtcct ggaggaaccc gagcaggggg ctgtgcccga ccaggggcag
      121 ggcgaacgtc tcgctgaaca gctccccgta ggcctcggcg atcgtggtca gcaggatcac
      181 gaagccccag aaggtgaaga agtgggccag tccggggatg ggccgcttga gcagcttgcg
      241 ctggacgaag acctcgacca gctcggcccg cagccgggtc caggccccct cgaagcggtc
      301 gggggctggc tggccgctgg cgatcagccg ggtgatccgc tcggcccgct ggccggccag
      361 ggcgacggcg aagaaggtca gcacggcccc gagggcgagc ttcatcgcac gtcctcctgg
      421 cctggtcgct tcgaatggct gcccgaagtc taccgctcgg taacatccgg aggcggatag
      481 gctgggcgga tgcgcgatcc gacccagccc cagtcccggc ccagccgtgc ccgggtgggc
      541 ctcctggccg gtggcctgct gctggtggtg gcgggggtgg ccgtgctggc ctgggtgctg
      601 gcctcgtcgg ccggcctggt cgggcgggac gaccccgtca tggccgaccc accggcgacc
      661 accaccccgg ccgcctccgg ggccgcccgc accgtcacct accggctgga gccggccggt
      721 ggcgaagagg cccggacggt gcggctggag atcgccgcca cccccgccgc ccgggcccgg
      781 gggctgatgg ggcgcaccga ggtgccggag gggaccggca tggtcttcct ctaccctgag
      841 gacgtggccg aggcctactg gatgaagaac accctggtcc cgctgtcgat cgcgttcgtg
      901 gccgccgacg gtcgggtggt cagcgtgtcc gagatgacgc cgtgcagggc cgacccgtgc
      961 cccagctacg cgccggccgg cccctaccgc tacgcggtcg agctggccgc cggctccttc
     1021 gccgccgccg gggtcggacc gggcgccaag gtggtccccg aggaccccgc agcgctccct
     1081 gagcctgcct gaacgcgcag gtcagaggca acataaagcg gctgcaccca taagattgac
     1141 aacaggccac tcaactccct atcctgggtt tcgaaacctg tcccagcttg tttgaggtga
     1201 tgagatggcc aaggccgtag ggatcgacct ggggaccacc aactccgtcg tgtccaccct
     1261 ggagggtggc gagccgacgg tgatccccaa cgccgagggg gcccgcacga ccccgtcggt
     1321 ggtcgccctc gccaagaccg gtgaggtgct cgtcggcgag gtcgccaagc gccaggcggt
     1381 caccaacccc gaccggaccg tgcgttcggt caagcgccac atgggtgagt cgtcctggcg
     1441 gttccgcgcc gacggcaagg agttctcccc ccagcaggtg agcgccttca tcctgcagaa
     1501 gctcaagcgc gacgccgagg cctacctggg cgacaccgtc acccaggccg tgatcaccgt
     1561 cccggcctac ttcgacgacg cccagcgcca ggccaccaag gaggccggtg agatcgccgg
     1621 gctcgacgtc ctgcgcatca tcaacgagcc gaccgcggcc tcgctcgcct actccctgga
     1681 caaggagaac gacgagacca tcctggtctt cgacctgggc ggcggtacct tcgacgtctc
     1741 cctgctcgac atcggcgagg gcgtcatcga ggtccaggcg acctccggcg acatgcacct
     1801 gggcggcgac gactgggacc agcgcatcgt cgagtggctg ctgaccacct tcaagaacaa
     1861 ccacggcatc gacctgtcga acgacaagat ggccatgcag cgcctcaagg aggcggccga
     1921 gaaggccaag atcgagctct cctcgaccct ggagaccacg atcaacctgc ccttcatcac
     1981 cgccaccccc gagggcccgc tccacatgga gcagcgcctc acccggggcg agttggagcg
     2041 catgacctcc gacctggtcg agcggctgcg cggccccttc gacaaggccg tgaccgactg
     2101 gaaggccaag acccagaaga gcctgaccga catccaccac gtggtgctgg tcggcggctc
     2161 gacccggatg cccatggtgg tcgagttcgt caagcagctc accggcggca aggagcccca
     2221 caagggcgtc aac
//
LOCUS       DASSWR010000252         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_37915,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000252 DASSWR010000000
VERSION     DASSWR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37915"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFO47_04290"
     CDS             <1..586
                     /locus_tag="VFO47_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET8787947.1"
                     /translation="LLGLLVLVLAYFAVTTTLVARWMGKDERPKVDAIVVLGAAQYDG
                     RPSAIYEARLEHAVQLWRDGVAPLLVFTGGKEEGDRFTEGGSGARWAIQRGVPSRAVL
                     TEEGSRTTYQNLAGAEQALERRNPGAGRHRIVVVSDPFHMFRAVKQAADLGMDAYPSP
                     TRTSPLSASRLKLTELVVREDLAIAGYLLSGAGK"
     gene            597..1214
                     /gene="upp"
                     /locus_tag="VFO47_04295"
     CDS             597..1214
                     /gene="upp"
                     /locus_tag="VFO47_04295"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET8787948.1"
                     /translation="MALTVVDHPLAGTLLTALRDETTPPPLFRLLAKRLSLVLAVEAT
                     RGVPTAARTVTTPLAEAEGTAFDAHLVAVPILRAGLGMLEAVTELFPEVPVGYLGLER
                     DHATFQPSTYYSKLPEIGGGYVLLLDPMLATGGSAAAACASLFAAGPARVTLLSVVAA
                     PEGIRRLEAEHPALDVVTAAVDERLNDQAYIVPGLGDFGDRLFGT"
     gene            1265..2440
                     /locus_tag="VFO47_04300"
     CDS             1265..2440
                     /locus_tag="VFO47_04300"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET8787949.1"
                     /translation="MARAQPPRVGEELLAPFRVLAGGEDLATAGPALLERACQALEAV
                     EGVLLLEDGGLVPGPSWGRPGRDGPLGRRPGDQALAAAAAGSGQPGTAGPGAVEAWCP
                     MVVEGRAVGVLALRRPAGRPFAGGDLAAVELLATGAALAADRLRFLRLWTRKLEQADA
                     AHSQLLRYADDLRTTFSAERRRAEELGEALDGLELAYTATVRALASAVEAKDAYTGGH
                     LSRVTQYGAEACRALGGDLATTPGLEYAFLLHDLGKIGVPDAVLNKAGPLTDEEWALM
                     REHPAIGLRILEGVPHMDVVRAVVCSHHERWDGAGYPEGRKGEQIPLAARVFAAVDAF
                     DAITTDRPYRAAASLDEALHRLREASGTQFAPDAVDAIHLVDRGRLAAIQATASRGR"
BASE COUNT          337 a         1082 c         1058 g          345 t
ORIGIN      
        1 cctgctgggc ctcctcgtgc tcgtgctcgc ctacttcgcc gtgaccacta cgctggtcgc
       61 ccggtggatg ggcaaggacg agcgtcccaa ggtcgacgcc atcgtggtcc tgggggcggc
      121 ccagtacgac gggcggccgt cggccatcta cgaggcccgc ctggagcacg ccgtccagct
      181 gtggcgggac ggggtggccc cgctgctggt gttcaccggg ggcaaggagg agggcgaccg
      241 cttcaccgag ggcgggtcgg gggcccgctg ggccatccag cgcggcgtgc cctccagagc
      301 ggtgctgacc gaggaaggca gccgcaccac ctaccagaac ctggccgggg ccgagcaggc
      361 cctggagcgg cgcaacccgg gcgccggccg gcaccgcatc gtggtcgtgt ccgacccctt
      421 ccacatgttc cgggcggtca agcaggccgc cgacctcggc atggacgcct acccgtcgcc
      481 cacccggacc agccccctgt cggccagccg gctcaagctc accgagctgg tggtgcgcga
      541 ggacctggcc atcgccggct acctgctctc gggagccggg aagtaaggag gttcccgtgg
      601 ccctgaccgt cgtcgaccac cccctggccg ggacgttgct gaccgcgctg cgggacgaga
      661 cgaccccgcc gccgctgttc cggctgctgg ccaagcggct gtcgctggtg ctggccgtgg
      721 aggcgacccg gggggtcccc acggcggccc ggacggtgac cacccccctg gccgaggcgg
      781 agggcaccgc cttcgacgcc cacctggtgg cggtgccgat cctccgggcc ggcctgggca
      841 tgctggaggc cgtgaccgag ctgttccccg aggtgccggt cggctacctg ggcctggagc
      901 gcgaccacgc caccttccag ccctccacct actacagcaa gctgcccgag atcgggggcg
      961 gctacgtgct gctgctcgac ccgatgctgg ccaccggcgg gtcggcggcg gcggcctgcg
     1021 ccagcctgtt cgcggccggg ccggcccggg tcaccctgct gtcggtggtg gccgcgcccg
     1081 agggcatccg gcggctggag gccgagcacc cggccctgga cgtggtcacc gccgcggtcg
     1141 acgagcgcct caacgaccag gcctacatcg tgcccgggct gggcgacttc ggggaccggc
     1201 tgttcgggac ctgatcgagc acgattcaat gtctcgaacc tgacggacaa gatgaggaga
     1261 gcacgtggct cgagcgcagc cacctcgcgt cggtgaggag ctgctggccc cgttccgggt
     1321 cctggcgggc ggcgaggacc tggccacggc cggccccgcc ctgctggagc gcgcctgcca
     1381 ggccctcgag gcggtcgagg gcgtgctcct gctggaggac gggggcctcg tccccgggcc
     1441 ctcctggggc cggccgggcc gggacgggcc gctcgggcgc cggcccggcg accaggcgct
     1501 ggccgcggcc gcggccggct ccggccagcc gggaacggcc gggcccgggg ccgtcgaggc
     1561 ctggtgcccg atggtggtcg agggccgggc ggtgggcgtg ctcgccctgc gccggcccgc
     1621 cgggcggccc ttcgccggcg gcgacctggc cgccgtggag ctgctggcca ccggggccgc
     1681 gctggccgcc gaccggctgc gcttcctgcg gctgtggacg cgcaagctcg agcaggccga
     1741 cgccgcccac tcccagctgc tgcgctacgc cgacgacctg cggaccacct tctcggccga
     1801 gcggcggcgg gccgaggagc tgggcgaggc cctggacggg ctcgagctgg cctacacggc
     1861 caccgtgcgc gccctggcca gcgccgtcga ggccaaggac gcctacaccg gcggccacct
     1921 ctcccgggtc acccagtacg gggccgaggc ctgccgggcc ctcggcggcg acctggccac
     1981 gacccccggc ctggagtacg ccttcctcct ccacgacctg ggcaagatcg gcgtgcctga
     2041 tgccgtcctc aacaaggccg ggcccctgac cgacgaggag tgggccctga tgcgcgagca
     2101 cccggccatc ggcctgcgca tcctcgaggg ggtgccgcac atggacgtgg tccgggcggt
     2161 cgtctgctcc caccacgagc gctgggacgg ggccggctac ccggagggcc gcaagggcga
     2221 gcagatcccc ctggccgccc gggtgttcgc ggccgtcgac gccttcgacg ccatcaccac
     2281 cgaccgcccc taccgggccg ccgccagcct cgacgaagcc ctccaccggc tgcgcgaggc
     2341 ctccggcacc cagttcgccc ccgacgccgt cgacgccatc cacctggtcg accggggacg
     2401 gctggcggcg atccaggcga ccgcgagccg cgggcgctag ggatgcggcg cctgcgggtg
     2461 cagctcaccc gcgctcagcc gcgagttcgt caccatggag acgagccggc gcgcccacct
     2521 gctcaccggc gaccaggcgt cgttgcaggc cggcctgcgg gccgaggtcc gggccgacaa
     2581 gggcttcgac cgggtggccg ccctgagccg gcgccctgga ggcggccctg cgcctcgagg
     2641 atgccgagcg ggccgccggc gaggccaggg cgggtgttct acgccatcgc cgacggggtg
     2701 ctcctgaccg acccgttcgg gcgggtcacg gccatgaacc gggccctgga ggcgctcacc
     2761 ggctggaccg aggccgaggc cagggccgcc cctacgccga ggtcctgccg atcgccaccg
     2821 ac
//
LOCUS       DASSWR010000253         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_857622,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000253 DASSWR010000000
VERSION     DASSWR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857622"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFO47_04305"
     CDS             <1..931
                     /locus_tag="VFO47_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HET8787950.1"
                     /translation="AHRGRGPALTPSPGGGAPLGRVTDPSQVVGAVAHSPLVVGPGAF
                     DAATGRLVRVGEVEVDGPVLDLWRAPTDNDQGHHGEALAPVWRRVGLHRLRHRVDGVA
                     LEGDGLVVRSRVAPAATDLGVAVAYRWSVVEGGLRLEVEAVPEGDWPCPWPRPGLRMA
                     VPAGFRKVEWFGRGPGEAYADTGRAARVGRFAATVAELQTPYLYPQENGNRRDVRWAT
                     VGDGGGRGIRFEGEPTFDLTVRPWTSEQLDQARHPTDLVPGDRVWVNLDLAQQGIGSA
                     SCGPGVLPAYRLDPAPATFAVRLLPAREGPMGS"
     gene            1504..2205
                     /locus_tag="VFO47_04310"
                     /pseudo
     CDS             1504..2205
                     /locus_tag="VFO47_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(2144..>2368)
                     /locus_tag="VFO47_04315"
     CDS             complement(2144..>2368)
                     /locus_tag="VFO47_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8787951.1"
                     /translation="DGHEIGGIMGVPGVSSGWTTLFEVADTDAAVARAAEAGGTAGTP
                     EDFVYGRLATITDPFGTEFSVGSRPEPQRA"
BASE COUNT          271 a          849 c          939 g          309 t
ORIGIN      
        1 cgcccaccgg ggtcgcggcc cggcgctcac cccaagcccc ggcggcgggg ccccactcgg
       61 gagggtaacc gacccgagcc aggtggtggg ggcggttgcc cacagccccc tggtggtggg
      121 acccggggcc ttcgacgcgg ctaccgggcg gctggtgcgg gtcggggagg tcgaggtcga
      181 cgggccggtg ctggacctgt ggcgggcgcc gaccgacaac gaccaggggc accatgggga
      241 ggcgctggcg ccggtgtggc ggcgggtcgg gctgcaccgg ttgcggcacc gggtggacgg
      301 ggtcgcgctg gagggcgacg gcctggtggt gcggtcccgg gtggcgccgg ccgcgaccga
      361 cctgggggtg gcggtcgcct accggtggtc ggtggtggag ggcgggctgc ggctggaggt
      421 ggaggccgtg cccgaggggg actggccctg cccgtggccg cggccggggc tgcggatggc
      481 ggtgccggcc gggttccgga aggtcgagtg gttcggccgc gggcccggcg aggcctacgc
      541 cgacaccggc cgggcggccc gggtcggccg cttcgcggcc acggtggcgg agctccagac
      601 cccgtacctg tatccgcagg agaacggcaa ccgccgcgac gtgcgctggg ccactgttgg
      661 cgacggcggc gggcgcggga tccggttcga gggggagccg accttcgacc tgaccgtccg
      721 gccctggacc agcgagcagc tcgaccaggc ccgccacccc accgacctcg tccccggcga
      781 ccgtgtctgg gtcaacctcg acctcgccca gcaggggatc ggctcggcct cctgcggccc
      841 cggcgtcctc cccgcctacc ggctcgaccc ggccccggcg accttcgcgg tccggctgct
      901 ccccgctcgg gaaggcccga tggggagctg acggcgcccc gtccccgttg ggccgcccga
      961 cgggcagggg tacggtggaa ggtgatggct gggacgtcct ggtggtcggc gccgcgacgg
     1021 ctgcccgcgc cggtggtgga cgcgggcctg gcccttggcc tcgctgtcgc ggtgatcgtc
     1081 gcgatcgagg tgacgaccgt cggcgagggc gtcgagccgg ctcccggcgc ccgctccctg
     1141 ggactgatca tggccgccct ggtgctggtg cggcggcgct ggcccctggg cgtgctgctc
     1201 gcctcggccg tggccctgca gctctacggc ctgaccaact acccgggcat cttccccgcg
     1261 gtgccgctgt cggtcgccgt ggcgaccgcc tgggcggccg ggcaccagcg ctggaccctg
     1321 gccgtcgtcg cctggttcgt ggtcgcgccc ttcgcgtacc tcctgtacca gctcgaggcg
     1381 gagaccgagc cgctgatgcc gctgctgaac gggaccgtgc agagcgccgc catgttcgcc
     1441 gccgtgctga tcctcgggga ggcgatgcgc aaccgtcgcg ccctggaccg ggcgaaccgc
     1501 ctgctccggg ccgagcagga ccgctccgag cagctgctgc gcaacgtcct cccgggcccg
     1561 atcgccgacc ggctcaagcg gggcgaggag gtcatcgccg acgggttccc ggaggtcacg
     1621 gtgctcttcg ccgatctcgt ggacttcacc cggcgcagcc agcgcagcag cccggaacgg
     1681 gtggtgcagg tcctggacga cctgttctcg gccctggacc ggctggccga gcgccacggg
     1741 ctggagaaga tcaagaccat cggcgacgcc tacatggtgg tcgggggcct gcccgagccc
     1801 cggcccgacc atgcccaggc ggtggccgag atggccctgg ccctgcgcga ggaggtcccc
     1861 cggcaccgcg acccggacgg ccggccgctg gccgtgcgcg tcggcatcga caccggcccg
     1921 gtggtggccg gggtgatcgg ccggcgcaag ttcagctacg acctgtgggg cgacaccgtc
     1981 aacacggcca gccggatgga gtcgcgcggg gtcgagggct gcatccaggt gacggaacgg
     2041 acctaccggc ggctccgcga ccgctaccgc ttccagcggc gggggccgat ccaggtcaag
     2101 ggcaagggga cacaggacac ttggttcctg gtcgcccggg cggtcaggcc cgctgaggct
     2161 cagggcgcga gcccaccgag aactcggtcc cgaacgggtc ggtgatggtg gccaggcgcc
     2221 cgtagacgaa atcctcgggt gtgccggcgg tgccgccggc ctcggcggcg cgggcgaccg
     2281 cggcgtcggt gtcggccacc tcgaacaggg tcgtccagcc cgacgacacg ccggggacgc
     2341 ccatgatccc gccgatctcg tgaccgtc
//
LOCUS       DASSWR010000254         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1366081, whole genome shotgun sequence.
ACCESSION   DASSWR010000254 DASSWR010000000
VERSION     DASSWR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366081"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFO47_04320"
     CDS             <1..402
                     /locus_tag="VFO47_04320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8787952.1"
                     /translation="LLLDPDPPRVLLGRRTRGQGRRYPGAWDGLGGKPDEGESLLEAL
                     AREASEEAGVEPLDPTALGCFHDGDRADAWFVVTSWKGEPRNREPSEHSELDWVPLHE
                     AADRPLTPTTRAAVARLAAMLATGHRLHRPR"
     gene            501..971
                     /locus_tag="VFO47_04325"
     CDS             501..971
                     /locus_tag="VFO47_04325"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET8787953.1"
                     /translation="MAIQDPPSGARATTRPAGPAPTEEPSTGELLKGIADDATKLVRQ
                     EILLARQELQEGLTASAKASALLVAAGVLGLYAFGFLLYTIGEAIGGPRWLGFAIVTL
                     VLLIVVAVLALVGKKRLAASKVAPEKAKAELQTTANELKEEITWGKPQQQPPGR"
     gene            989..1186
                     /locus_tag="VFO47_04330"
     CDS             989..1186
                     /locus_tag="VFO47_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787954.1"
                     /translation="MEDKVAKLSERAPEEARKLIKRVAFAVLTAVAVLATRKLVDKLW
                     ERATGELPPTKVMREDEADED"
     gene            1344..>2381
                     /locus_tag="VFO47_04335"
     CDS             1344..>2381
                     /locus_tag="VFO47_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014362209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8787955.1"
                     /translation="MWAMAMKEFRQLRRDRRTLAMMILMPVLLLVVFGYAASFDVDRV
                     RTVVVGPRAEQVAAALPERLEVVRVAPGEGRKAAVEALRDAEAAVALVAGDAGRSDVL
                     VDGADLFSARAVVTELRGRPGLPAPEVLFNPGLETSAIMVPGLMGVVLVFVGTIATAL
                     GVVRERQSGTLEQLAVMPFRPRDVLGGKLLPYLGVAVVDLAVIVAVGVLLFDVPFAGS
                     PLVFALGALLFLFVTVGIGVLISTVSETQGQAIQLAIMTMLPQILLSGLIFPLQAMAP
                     GVRWIGYLLPLTWFIQLARGVMVRGAPFEALVFPLAMLAVLGAVVFGASVARFRRDLA
                     PSGRGHRGGEAG"
BASE COUNT          300 a          842 c          883 g          356 t
ORIGIN      
        1 ctcctgctcg acccggaccc gccgcgggtc ctgctcggcc gccgcacccg cggccagggc
       61 cgccgctacc cgggagcctg ggacgggctc ggcggcaagc ccgacgaggg cgagtcgctg
      121 ctggaggccc tggcccgcga ggcgtccgaa gaggccgggg tggagccgct cgacccgacc
      181 gccctcggct gcttccacga cggtgaccgg gccgacgcct ggttcgttgt cacctcctgg
      241 aagggcgagc cgcgcaaccg cgagccgtcc gagcacagcg agctcgactg ggtgccgctg
      301 cacgaggccg ccgaccgccc cctgaccccg accaccaggg cggcggtggc ccggctggcg
      361 gccatgctgg cgaccggcca tcgactgcac cgcccccggt gatgtgcgat aactcttgcg
      421 gcggcggggt aggaacgtcg tggggcggca ggcctcgccg tcccacgacc ccaacgagac
      481 gacggtaagg ggggactgcc ttggcaatcc aggacccgcc ttcgggcgcg cgggcgacca
      541 cgcggccagc cgggccggcg cccaccgagg agccatccac cggtgagctg ctgaagggca
      601 tcgccgacga cgcgaccaag ctcgtgcggc aggagatcct gctcgcccgg caggagctcc
      661 aggaggggct caccgccagc gccaaggcga gcgccctgct cgtcgcggcc ggtgtgctcg
      721 ggctgtacgc cttcggcttc ctcctctaca ccatcggtga ggcgatcggc ggcccgcgct
      781 ggctcgggtt cgcgatcgtg accctggtgc tgctgatcgt ggtcgcggtg ctcgcgctgg
      841 ttggcaagaa gcgcctggcc gcctccaagg tcgctccgga gaaggccaag gccgagctcc
      901 agacgaccgc caacgagctg aaggaggaga tcacgtgggg gaaacctcag cagcaaccgc
      961 ccgggaggta gaggagaccc gcaagcagat ggaggacaag gtggccaagc tctccgagcg
     1021 cgcgccggag gaggcccgca agctgatcaa gcgggtcgcg ttcgcggtcc tcaccgcggt
     1081 cgccgtgctg gcaacccgca agctggtcga caagctctgg gagcgggcca cgggcgagct
     1141 tccgccgacc aaggtcatgc gcgaggacga agccgacgag gactgagcga ccagcgcgtc
     1201 gcgctggtcg cagggcgccg gcgagccggc gccctcttta ttggagttcc ttctgatttg
     1261 ctccgccggc gtcttgcccg aggatttcat tcatcgtaga cttcatccga taatgaattc
     1321 cgggtcacgg aggtgccgcc ggcatgtggg ccatggccat gaaggagttc cgccagctgc
     1381 gccgggaccg gcgcacgctg gccatgatga tcctgatgcc ggtgctgctg ctggtggtgt
     1441 tcggctacgc cgccagcttc gacgtcgacc gggtgcggac ggtggtcgtc ggcccccggg
     1501 ccgagcaggt ggccgccgcc ctgccggaac ggctggaggt ggtccgggtc gctcccgggg
     1561 agggccgcaa ggccgcggtc gaggccctgc gcgacgccga ggcggcggtg gcgctggtgg
     1621 ccggcgacgc cggccggtcc gacgtgctgg tcgacggggc cgacctcttc tcggccaggg
     1681 cggtcgtgac cgagctgcgg gggcggcccg ggctgcccgc ccccgaggtg ctgttcaacc
     1741 cgggccttga gacctcggcg atcatggtcc ccggcctgat gggcgtggtg ctggtgttcg
     1801 tgggcacgat cgccaccgcc ctcggggtcg tccgcgagcg ccagagcggc accctggagc
     1861 agctggccgt gatgcccttc cggccccgcg acgtgctcgg cggcaagctc ctgccgtacc
     1921 tgggggtggc ggtggtcgac ctggcggtga tcgtggccgt gggggtgctg ctgttcgacg
     1981 tccccttcgc cggctcaccg ctggtcttcg ccctcggggc cctgctgttc ctgttcgtca
     2041 ccgtgggcat cggcgtgctc atctcgaccg tgtccgagac ccagggccag gccatccagc
     2101 tggccatcat gaccatgctc ccccagatcc tgctgtccgg gctgatcttc ccgctccagg
     2161 ccatggcccc gggggtccgc tggatcggct acctgctgcc gctcacctgg ttcatccagc
     2221 tggccagggg cgtgatggtc aggggggccc cgttcgaggc cctggtgttc ccgctggcca
     2281 tgctggccgt gctcggggcg gtggtgttcg gcgcgtcggt cgcccgcttc cgccgcgacc
     2341 tggccccctc cggccgcggc caccgcggcg gggaggccgg c
//
LOCUS       DASSWR010000255         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1111552, whole genome shotgun sequence.
ACCESSION   DASSWR010000255 DASSWR010000000
VERSION     DASSWR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111552"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1892
                     /locus_tag="VFO47_04340"
     CDS             81..1892
                     /locus_tag="VFO47_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8787956.1"
                     /translation="MGRGLAASAPTYGTPPDTGDRPRDRRSGRRARAWLWPLVRPHRR
                     LVALASVAVLVQTGAALAMPYLVKVAIDEGVVPKDLAVINRVALLYLALAGVQFVAGR
                     FEIEAVARAGQRVLYTVRTKLFRHLQTLSLDFYERERTGRLVARMTADIDAMSDLVTD
                     GLVTLVTGIVTMVGVAVILVVMDWKLALATLVVAPLIGLAARFWRRWSADAYRQVRET
                     HSVVTVQLQESLAGVRAIQSFRRERATAARLAEANHAEQVAHWRTIGLASFFFPGIEF
                     LGTAATVVVLGVGGQRVLAGDLDIGVLAAFLLYLRNLFDPVQQLSELYDSFQSATAGA
                     ERVGTVLAEQPSVREAPDPVPLPDPRGDVRLEDVCFAYDQGPEVLHDVDLRVDAGSTL
                     ALIGPTGAGKSTVAKLIARFYDPLGGRVTLDGVDLRQVRLADLRRAMGYVPQEGFLFS
                     GTVLDNIRFGKPDAARAEVEAAARAVGADQVIAGLPDGYDTHVGERGALLAAGERQLV
                     AFARAWIADPALLILDEATSNLDAASEARITDALQRLRSGRTIIIIAHRLSTIAQADQ
                     IAVVEDGRIVEAGTPADLRARGGRFAELFDRWMAGAA"
     gene            complement(1905..>2198)
                     /locus_tag="VFO47_04345"
     CDS             complement(1905..>2198)
                     /locus_tag="VFO47_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8787957.1"
                     /translation="GNAMLAPTVTRRLIAEFAARGGTGSAAARKRLEALTGRELEVVV
                     AVAQGLSNAQIGRRLFMSEATVKAHVSSILAKLGMTNRVQAAILAHDAGLAGT"
BASE COUNT          254 a          838 c          796 g          310 t
ORIGIN      
        1 tggatcggga ggggtccggg gaaccccgag ggggtgcccc ggtgagtaga agacgaggcg
       61 tgggcttccg cggagggtcc atggggcggg ggctggccgc ctccgcgccc acctacggga
      121 cgccgccgga caccggggac cggccgcgcg accggcggtc ggggcggcgg gcccgggcct
      181 ggctgtggcc gctggtccgt ccccaccgcc ggctggtcgc cctggcctcg gtggccgtgc
      241 tcgtccagac cggcgccgcc ctggccatgc cctacctggt caaggtggcc atcgacgagg
      301 gcgtggtccc caaggacctg gccgtgatca accgggtcgc cctcctctac ctggccctgg
      361 ccggtgtgca gttcgtggcc ggcaggttcg agatcgaggc ggtggcccgg gccggccagc
      421 gggtgctgta cacggtccgg accaagctgt tccgccacct ccagaccctg tcgctcgact
      481 tctacgagcg cgagcggacg ggcaggctgg tcgcccgcat gaccgccgac atcgacgcca
      541 tgagcgacct ggtcaccgac ggcctggtca cgctcgtcac cgggatcgtc accatggtcg
      601 gggtggcggt gatcctggtc gtgatggact ggaagctggc cctggccacc ctggtcgtcg
      661 cccccctgat cggcctggcc gcccgcttct ggcggcgctg gtcggccgac gcctaccgcc
      721 aggtcaggga gacccacagc gtggtcaccg tccagctcca ggagtcgctg gccggggtgc
      781 gggcgatcca gtcgttccgg cgcgagcggg ccaccgccgc ccgcctggcc gaggccaacc
      841 acgccgagca ggtcgcccac tggcgcacca tcggcctggc ctcgttcttc ttccccggca
      901 tcgagttcct gggcacggcc gccaccgtgg tcgtcctcgg cgtcggcggc cagcgggtgc
      961 tcgccgggga cctggacatc ggcgtcctgg ccgcgttcct cctctacctc cggaacctgt
     1021 tcgacccggt gcagcagctc tccgagctgt acgacagctt ccagtcggcc accgccgggg
     1081 ccgagcgggt cgggaccgtc ctggccgagc agccctcggt ccgcgaggct cccgatcccg
     1141 tccccctccc cgacccgcgc ggcgacgtcc ggctcgagga cgtctgcttc gcctacgacc
     1201 aggggccgga ggtcctgcac gacgtcgacc tccgtgtgga cgccgggtcg accctggccc
     1261 tgatcgggcc gaccggggcg ggcaagtcga ccgtggccaa gctgatcgcc cgcttctacg
     1321 acccgctggg tggccgggtg accctcgacg gggtcgacct gcgccaggtc cgcctggccg
     1381 atctgcgccg ggccatgggc tacgtccccc aggaggggtt cctgttctcg ggcacggtcc
     1441 tggacaacat ccgcttcggc aagcccgacg ccgcccgggc cgaggtcgag gccgccgccc
     1501 gggcggtcgg ggccgaccag gtcatcgccg gcctgcccga cggctacgac acccacgtgg
     1561 gcgagcgggg ggcgctgctg gcggccgggg agcgccagct ggtcgccttc gcccgggcct
     1621 ggatcgccga cccggccctg ctcatcctgg acgaggccac cagcaacctg gacgcggcca
     1681 gcgaggcccg catcaccgac gccctccagc gcctccgctc cggccggacc atcatcatca
     1741 tcgcccaccg gctgtccacc atcgcccagg ccgaccagat cgccgtggtc gaggacggcc
     1801 gcatcgtcga ggccggcacc ccggccgacc tccgggcccg cggcggccgc ttcgccgagc
     1861 tgttcgaccg ctggatggcc ggcgcggcct gagggccggg tcgcctaggt gccggcgagg
     1921 ccggcgtcgt gggccaggat ggcggcctgg acccggttgg tcatgccgag cttggccagg
     1981 atgctgctga cgtgggcctt gaccgtggcc tcgctcatga acaggcgccg gccgatctgg
     2041 gcgttgctca ggccctgggc caccgcgacc accacctcga gctcgcgccc ggtgagggcc
     2101 tccagccgct tgcgggccgc cgccgacccg gtgccgcccc gggccgcgaa ttcggcgatc
     2161 agccgcctgg tcacggtggg agccagcatg gcgttgcc
//
LOCUS       DASSWR010000256         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_715223,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000256 DASSWR010000000
VERSION     DASSWR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715223"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..2165)
                     /locus_tag="VFO47_04350"
     CDS             complement(123..2165)
                     /locus_tag="VFO47_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8787958.1"
                     /translation="MSQPLRYTIRKTLRLLLIFGILYALLIPILYMMYRSNRGLDWWN
                     TLGGDPRVAPEYRGYLIRTDHPLAPEFNLWKATWALPFAIAGMALYLMFIMSAMVLQF
                     VAIFWFLARGQTYVIYPREYDVTFDDVRGQPEIVESTKEALTLFQGFKKFRDAGGYPP
                     HGILFEGPPGTGKTLLGKAIAGSANVPFVYASGTSFNNMFMGIGNLRIMRLFKKARKL
                     SRKYGGAVIFLDELDAVGGTRGAVSTASEPADTTRHWLAKGPLRIIMGGMGMGGAGMY
                     VNELLVQMDGMIMPKGLWRHVKRILHLGKPKIPQYNVMVIGATNQASTLDPALLRPGR
                     FDRKLHVGLPSGPGREDILQYYLDKVPHDPVDISRLARATYGFSPAQIKNLVNEALIF
                     ALVDGREKLNFDDLWTAKVTEEIGLKEATKTSARDRQMTAFHEAGHAVLAYILELDDQ
                     IQVASIIKRRDTLGVVYRTPLEERHTELREDMRRDIVVALGGLAAEEIWFGETTTGPS
                     SDLRHATRQAAIMLGLLAMGDDLVSYGVLPQTPYNEGPLAAILGNPDSRKAIGELLAE
                     AKQQAVELLRANEPAVRALSERLLEKDEVAGEELEELMQSKAVARATPAWRPVLELVG
                     DTWHTPALLEASPRDSGATGPTISPPREPRPGAQPGTGLGNPSTPSRSDGGGEFEW"
BASE COUNT          340 a          863 c          888 g          399 t
ORIGIN      
        1 gttcgtcaac ctcgacggca acgccatcat caaccgcggc cagccgccgt cgggcgccca
       61 ctccgacatc ttccaccccg agctcgtctg ggccggcctg gccgcggccg ggctggtcta
      121 gctcaccact cgaactcgcc gccgccgtcc gagcgggacg gggtgctcgg gttgccgagg
      181 ccggtgccgg gctgggcgcc ggggcggggc tcgcgcgggg gtgagatcgt cgggccggtg
      241 gcgccgctgt cgcggggcga ggcctccagc agggccgggg tgtgccaggt gtcgccgacc
      301 agctccagca ccggccgcca ggccggggtg gccctggcca ccgccttgga ctgcatcagc
      361 tcctcgagct cctcgccggc gacctcgtcc ttctccagca gccgctccga cagggcccgc
      421 acggccggct cgttggcccg cagcagctcg accgcctgct gcttggcctc ggccagcagc
      481 tcgccgatgg ccttgcggct gtcggggttg ccgaggatgg cggccagcgg cccctcgttg
      541 tagggggtct ggggcaggac cccgtaggag acgaggtcgt cgcccatggc cagcaggccc
      601 agcatgatgg cggcctgccg ggtggcgtgg cgcaggtcgg agctgggccc ggtggtggtc
      661 tcgccgaacc agatctcctc ggcggccagc ccgccgaggg cgaccacgat gtcgcggcgc
      721 atgtcctcgc gcagctcggt gtggcgctcc tccagcggcg tccggtagac caccccgagg
      781 gtgtcgcgcc gcttgatgat cgaggccacc tggatctggt cgtccagctc caggatgtag
      841 gcgagcacgg cgtggccggc ctcgtggaag gcggtcatct ggcggtcccg ggccgaggtc
      901 ttggtcgcct ccttgaggcc gatctcctcg gtcaccttgg ccgtccacag gtcgtcgaag
      961 ttgagcttct cgcgcccgtc gaccagggcg aagatcagcg cctcgttgac caggttcttg
     1021 atctgggccg ggctgaaccc gtaggtcgcc ctggccagcc gcgagatgtc gaccgggtcg
     1081 tggggcacct tgtccaggta gtactggagg atgtcctcgc ggcccggccc cgagggcagg
     1141 ccgacgtgca gcttgcggtc gaagcggccc ggccgcagca gcgccgggtc aagggtcgag
     1201 gcctggttgg tggccccgat gaccatgacg ttgtactggg ggatcttggg cttgccgagg
     1261 tgcaggatcc gcttgacgtg ccgccacagc cccttgggca tgatcatgcc gtccatctgg
     1321 accagcagct cgttgacgta catgccggcg ccgcccatgc ccatgccgcc catgatgatc
     1381 cgcagcggcc ccttggccag ccagtggcgg gtggtgtcgg cgggctcgct ggcggtggag
     1441 accgccccgc gggtcccgcc caccgcgtcc agctcgtcca ggaagatcac ggccccgccg
     1501 tacttgcgcg acagcttccg ggccttcttg aacagccgca tgatgcgcag gttgccgatg
     1561 cccatgaaca tgttgttgaa gctggtcccc gaggcgtaca cgaacggcac gttggccgac
     1621 ccggcgatcg ccttgccgag cagggtcttg cctgtgcccg gcgggccctc gaacaggatc
     1681 ccgtggggcg ggtagccgcc ggcgtcgcgg aacttcttga acccctggaa cagggtgagg
     1741 gcttccttgg tcgactcgac gatctcgggc tggccgcgga cgtcgtcgaa ggtgacgtcg
     1801 tactcgcgcg ggtagatcac gtaggtctgg ccgcgggcca ggaaccagaa gatggccacg
     1861 aactgcagga ccatcgcgga catgatgaac atgaggtaca gggccatgcc cgcgatcgcg
     1921 aacggcagcg cccaggtcgc cttccagagg ttgaactcgg gcgccagggg gtggtcggtg
     1981 cggatcaggt agccccggta ctcgggggcg acccgggggt cgccgcccag ggtgttccac
     2041 cagtccaggc cgcggttgga ccggtacatc atgtacagga tcgggatcag cagggcgtag
     2101 aggatgccga agatcagcag cagccgcagg gtcttccgga tcgtgtatcg gagtggctga
     2161 ctcacggacc cccgccttcc ctgccgctcg ttgttgcctg ccgacctgcg cacactgcct
     2221 acgagtatga ctcccggctc ctgataccgc tacaggagag gccaccctct ggccgggcgt
     2281 gggggacagg ccatagactg ccagcctgac tacccaggag gttgcttcgc tcgtgcccgg
     2341 cctcgcggtc atcgcccata ccgtctgggg ggcagccggg cccagcctcc acgatccccg
     2401 caaccgcctc ggcgacgccc tcggccgccg ccacgacctg gccgtggccg tcgagagcgg
     2461 gcgcatcacc gccgtcgtcc cggccgccga
//
LOCUS       DASSWR010000257         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_8478,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000257 DASSWR010000000
VERSION     DASSWR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8478"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFO47_04355"
     CDS             complement(<1..668)
                     /locus_tag="VFO47_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET8787959.1"
                     /translation="MRAAEVVERVSAPGVVQAAGQADLKAPAAARVERLVVKDGQEVA
                     EGDLVAELSSEQVDDQVRQAQAAVDAASSLGGAAPGLPTGAALSAFQQVQSQVTATSA
                     AVIEAMRAALPLVPRAQRARLQARIDRAQVRVADLQRQAAQAADAAAAAADAQAATLQ
                     QSIQAATAAQRGQAELALELARDQQERLTLRSPLDGTVQLGRSGSGATTIPQVEGLPE
                     GASEA"
     gene            complement(1038..1949)
                     /locus_tag="VFO47_04360"
     CDS             complement(1038..1949)
                     /locus_tag="VFO47_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET8787960.1"
                     /translation="MLKATLLKAAGSSFLRRQVTANPIARRVADRFIAGDTLDEAEHA
                     IRTLNTGGMSVALDYLGENTESEAQARQSTASYLAAIDRIQDRGMDANISVKLTALGL
                     DIRQELALEEASKVAARGKEVGAMVGVDMEAAIYAGRTLDIVEALKRDYDNVGVCVQS
                     YLYRSRDDLERLNRLGIPVRLVKGAYQESPEVAYPEKASVDHAYARMLDDLLARNPFP
                     MVATHDPAMVRLTKTLVAHHRRDRDTFEFQMLYGVRRDLQAQVVAEGYRLRVYLPYGT
                     QWYPYFMRRLAERPANLYFFLSNLVKR"
     gene            complement(1997..>2292)
                     /locus_tag="VFO47_04365"
     CDS             complement(1997..>2292)
                     /locus_tag="VFO47_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787961.1"
                     /translation="RYHRKALPSERHGDYMALEDADRVVVRRLAALLRVAEGLDADHF
                     QVVEAVAVVDGGSALRLALRARDTPDLWAAGHNADLFESEFGRPLVPVASEVA"
BASE COUNT          298 a          814 c          870 g          310 t
ORIGIN      
        1 gcctcggagg cgccctccgg aagcccctcg acctggggga tggtcgtggc cccgctcccc
       61 gagcggccga gctgcacggt gccgtccagc ggcgaccgca gggtcaggcg ctcctgctgg
      121 tcgcgggcca gctccagggc cagttcggcc tgcccgcgct gggcggcggt ggccgcctgg
      181 atcgactgct gcaaggtggc cgcctgggcg tcggccgcgg cggcggcggc gtcggccgcc
      241 tgggcggcct ggcgctgaag gtcggccacc cgcacctggg cccggtcgat gcgggcctgg
      301 agccgggccc gctgcgccct cggcaccagc ggcagggccg cccgcatggc ctcgatcacg
      361 gccgcgctgg tcgccgtcac ctgcgactgg acctgctgga aggccgacag cgcggccccc
      421 gtcggcagtc ccggcgccgc ccccccgagc gacgaggccg cgtccaccgc cgcctgggcc
      481 tgccgcacct ggtcgtccac ctgctccgac gacagctcgg cgaccaggtc cccctccgcg
      541 acctcctgcc cgtccttgac caccagccgc tcgacccgcg ccgccgccgg cgccttcagg
      601 tccgcctgcc ccgccgcctg caccaccccg ggggcgctga cccgctccac cacctcggcc
      661 gcccgcaccg gctccgccgc ataccgcccc gcatcccccg aatcgcaccc ggcgagcagc
      721 agcaccccag caagcccaag agcggcggca gccccggtca acccgtgccg catccgccac
      781 cctctcttca ccgataacac cccggccagc atagatgcgc ccgggtggtt ctgcgtcgac
      841 agccgcacac accatcgtgg aggtctgcct ggaggaggaa gggccaccgg aagcccttcc
      901 gctggtcacc tcgatcgatg tatcgatccc tgcgcgaccg acgggatccc tcgtggcgtc
      961 tccggccggc gggccgtcgc cgcgttgcag cggaccgacc gagtccacgc cgaggcggcg
     1021 agagggaagt ccgtcgttca gcgcttcacc aggttggaga ggaagaagta gaggttggcg
     1081 gggcgttcgg cgaggcggcg catgaagtag gggtaccact gggtgccgta ggggaggtag
     1141 acccggaggc ggtagccttc ggcgacgacc tgggcctgga ggtcgcggcg gacgccgtag
     1201 agcatctgga actcgaaggt gtcgcggtcg cggcggtggt gggcgaccag ggtcttggtc
     1261 aggcggacca tggccgggtc gtgggtggcg accatcggga aggggttgcg ggcgagcagg
     1321 tcgtcgagca tgcgggcgta ggcgtggtcg acgctggcct tctcggggta ggcgacctcg
     1381 ggcgactcct ggtaggcgcc cttgacgagg cggacgggga tgccgaggcg gttgaggcgt
     1441 tcgaggtcgt cgcggctgcg gtacaggtag gactggacgc agacgccgac gttgtcgtag
     1501 tcgcgcttga gggcctcgac gatgtcgagg gtgcggccgg cgtagatggc ggcctccatg
     1561 tcgacgccga ccatggcgcc gacctccttg ccgcgggcgg ccaccttgga ggcctcctcg
     1621 agggccagct cctggcggat gtccaagccc agggcggtca gcttgacgga gatgttggcg
     1681 tccatgccgc ggtcctggat gcggtcgatg gcggccaggt aggaggcggt ggactggcgg
     1741 gcctgggcct cggactcggt gttctcgccc aggtagtcga gggcaacgga catgccaccg
     1801 gtgttgaggg tgcggatcgc gtgctcggcc tcgtcgaggg tgtcgccggc gatgaaccgg
     1861 tccgcgaccc ggcgggcgat cgggttcgcg gtcacctgcc gccgcaggaa cgacgaaccc
     1921 gccgccttca gcaacgtcgc cttcagcatg gttcaaccat acctcgcggg cgggatgcgg
     1981 agagccccgg gggcggctac gccacctcgc tggcgacggg gaccaggggg cggccgaact
     2041 cggactcgaa caggtcggcg ttgtggccgg cggcccagag gtccggggtg tcgcgggccc
     2101 gcagggcgag gcggagggcg gagcccccgt cgaccaccgc gaccgcctcg accacctgga
     2161 agtggtcggc gtccaggccc tcggcgaccc gcaggagcgc ggccaggcgg cggaccacga
     2221 cccggtcggc gtcctccagg gccatgtagt cgccgtgccg ctctgacggc agggccttgc
     2281 ggtggtagcg gg
//
LOCUS       DASSWR010000258         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1055201, whole genome shotgun sequence.
ACCESSION   DASSWR010000258 DASSWR010000000
VERSION     DASSWR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055201"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..>2236
                     /locus_tag="VFO47_04370"
     CDS             213..>2236
                     /locus_tag="VFO47_04370"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET8787962.1"
                     /translation="MGLNEYKPGAAFPGVIGRTTSESSPAWPQPVRAVADAPNVLFIV
                     LDDTGFGQLGCYGSPIATPNLDALAAGGLLYTNMHTTALCSPSRSCIITGRNHHANAT
                     AAITELATGYPGYNGSIPFENGFLSEMLSQHGYNTYMIGKWHLMPSEFESAAGPYDRW
                     PLGRGFDRFYGFLGGDTSQWYPDLVADNHQVEPPKTPEQGYHLTEDLADKAIGFIADA
                     KQVAPHKPFFLHFCTGAAHAPHHVAKEWADRYQGQFDDGWDAYRERVFARQKELGIMP
                     ADAELSRHDPDVPDWDSLSPDARRLAARMMEVFAGFLSHTDHHIGRLLDFLKETGQFD
                     DTLIMLVSDNGASPEGGVTGTTNEGQFFNNAPEPLEDSLNKIDELGGPTTFNHYPWGW
                     TWAGNTPFRRWKRETYRGGACDPFLVHWPAGISAHGEVRDQYAHIIDMVPTVLDALGI
                     EPPATIRGVTQAPIHGVSFAPSFNDQAAPTRHTTQYFEMFGHRSIDHDGWRAVCPWPG
                     PSFTEAGRPFGTPITPEILTDLDARHWELYHVAEDPAENHNLAEQHRDILIGMIAQWY
                     VEAGKYDVLPIDGSALQRLMTERPQIAESRDRYVYRPGTQTVPFFAAPRLLNRPHAIT
                     ADADIPTGGAEGVLLCQGTRVGGWSFYIKDGKLHYAHNYVARTIYQVASA"
BASE COUNT          385 a          775 c          726 g          350 t
ORIGIN      
        1 gcttatgggc tgtcgagcga gtggagtcca ccaatgccgg gccgagcggc tgatggcgtt
       61 ggccttgaag cggccttgtc gaggacagct ggtcatggag ttgaactggc ctttcatccc
      121 gaaggggtga tgctcggtca ctggcgggag ggtctgatga cgtcgagagt tcgtctgggc
      181 tcgtggggct ttggcgaggg gagatgggtc gcatggggtt gaacgagtac aagccgggcg
      241 cggcgttccc gggggtgatc gggcggacga cgagcgagtc gagcccggcg tggccgcagc
      301 cggtgcgggc cgtggctgat gcccccaacg tcctgtttat cgtgttggat gacaccggat
      361 ttgggcagct gggctgctac ggcagcccga tcgcgacacc gaatcttgac gcgttggctg
      421 ccggggggct gctgtatacc aacatgcaca ccacggcgct gtgctcaccc tcgcgttcgt
      481 gcatcatcac cgggcgcaac caccacgcca acgcgaccgc ggcgatcacc gagctcgcca
      541 ctggctatcc cggctacaac ggcagcatcc cgtttgagaa cgggttcttg tccgagatgc
      601 tgtcccagca tggctacaac acctacatga tcggcaagtg gcacctgatg ccctcggagt
      661 tcgagtcagc cgccggcccg tacgaccgct ggccgctggg gcggggcttt gaccgcttct
      721 acggcttcct cggtggcgac accagccagt ggtatccgga cctggtcgcc gacaaccatc
      781 aggtcgagcc gcctaagacc cctgagcagg gctatcacct gaccgaggac ttggccgaca
      841 aggcgatcgg attcatcgct gacgccaagc aggtcgcccc gcacaagccg ttcttcctgc
      901 acttttgcac cggcgccgcc cacgccccgc accatgtggc caaggagtgg gctgaccgtt
      961 accaggggca gttcgacgac ggctgggacg cctaccggga gcgggtgttc gcccgccaga
     1021 aggaactcgg catcatgccg gccgatgccg agctgtcgcg ccacgatccc gatgtgccgg
     1081 actgggacag cctgtcgccg gacgcccgcc ggctggccgc ccggatgatg gaggtgttcg
     1141 ccgggttcct gtcccacacc gaccatcaca tcgggcggct gctggacttc ctcaaggaaa
     1201 ccggccagtt cgacgacacc ctgatcatgc tggtgtccga caacggcgcc agccccgaag
     1261 gcggggtcac cgggaccacc aacgaggggc agttcttcaa caacgcgccc gagccgctgg
     1321 aggacagcct caacaagatc gacgagctcg gcggcccgac caccttcaac cactacccgt
     1381 ggggttggac ctgggccggg aacaccccgt ttcggcggtg gaagcgggag acctaccggg
     1441 gcggcgcctg cgatccgttc ctggtccact ggccggcagg catcagcgcc cacggcgagg
     1501 tccgcgacca gtacgcccac atcatcgaca tggtcccgac cgtgctggac gccctgggga
     1561 tcgagccgcc ggccacgatc aggggcgtga cccaggcgcc gatccatggg gtcagcttcg
     1621 cccccagctt caacgaccag gccgccccga cccgccacac cacccagtac ttcgagatgt
     1681 tcggccaccg ctccatcgac cacgacggct ggcgggcggt gtgcccgtgg ccgggcccgt
     1741 cgttcaccga ggccggccgg ccgttcggca cgccgatcac gcccgagatc ctgaccgacc
     1801 tggacgcccg ccactgggag ctgtatcacg tcgccgaaga cccggccgag aaccacaacc
     1861 tggccgagca gcaccgcgac atcctgatcg gaatgatcgc ccagtggtat gtcgaggccg
     1921 gcaagtatga cgtgctgccg atcgacggca gcgccctcca gcgcctgatg accgagcgac
     1981 cacagatcgc cgaaagccgc gaccgctatg tctaccggcc cgggacccag accgtgccct
     2041 tcttcgccgc gccccggttg ctcaaccgtc cccacgccat caccgccgac gccgacatcc
     2101 cgaccggtgg cgccgaaggg gtgctgctct gccagggcac acgggtcggt ggctggtcgt
     2161 tctacatcaa ggatggcaag ctgcactacg cccacaacta cgtcgcacgc accatctacc
     2221 aggtggcgtc ggccga
//
LOCUS       DASSWR010000259         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1027479, whole genome shotgun sequence.
ACCESSION   DASSWR010000259 DASSWR010000000
VERSION     DASSWR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027479"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFO47_04375"
     CDS             <1..335
                     /locus_tag="VFO47_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8787963.1"
                     /translation="LFAGLSEAAAGREDRLANRPAGLAYALRRLGTGAQEPLWGRLAG
                     LRPPALLVAGALDAKFAGIAREMAAAIGPAARVALVPGAGHAVHLERPDAMAALLEAF
                     LDDPGARP"
     gene            complement(344..1207)
                     /locus_tag="VFO47_04380"
     CDS             complement(344..1207)
                     /locus_tag="VFO47_04380"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET8787964.1"
                     /translation="MNVWVQGARPRTLVAAVCPVLVGTAAAASEGPVVAWRALAALAV
                     AVALQIGVNYANDYSDGVRGADTPDRLGPARLTAAGLAAPAAVRRAAGVAFAVAALAG
                     LALAVAVDLRLLLVGVAAIAAAVLYTGGPRPYGYAGFGELAVLAFFGVVATCGSAYVQ
                     LERVPAAAAAASLVVGLGAVAILLANNVRDVDGDRLAGKRTLAVRLGRDRARDLFTAV
                     VAGMFGVAALLGLARPPVLVTLAAVPLAVAPVRLVRTRTDGPGLIAALGATARLQLAV
                     SLLLTAGLWWS"
     gene            complement(1221..2033)
                     /gene="menB"
                     /locus_tag="VFO47_04385"
     CDS             complement(1221..2033)
                     /gene="menB"
                     /locus_tag="VFO47_04385"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET8787965.1"
                     /translation="MEWQHQGSWTDIGYETAEGIAKITIRRPEVRNAFRPKTLFELQD
                     AFELARDDPSIGVIVLTGEGPDAFCSGGDQRIRGDDGYVDERGIGRLNVLDLQIQIRR
                     LPKPVIAMVAGYAIGGGHVLHLVCDLTIAADNARFGQTGPRVGSFDGGYGMGMLARTV
                     GRKKAAEVWLLCRQYDAAQALEMGLVNTVVPLADLERVTVEWCRRILEHSPLALRLLK
                     AGLNADTDGLAGVQQLAGDATLLYYMTSEAQEGRNAFREKRPPDFAKFPKRP"
     gene            2096..>2431
                     /locus_tag="VFO47_04390"
     CDS             2096..>2431
                     /locus_tag="VFO47_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787966.1"
                     /translation="MSSSVALVAVALPRPAAARAIVAAWEAGEAVLPLDPDAPAPELK
                     GILAAARPTHLLDTDGRTRLPEGAPAEPGLAAVVATSGTSGAPKMVELGADAVRWSAL
                     ATTAALEAAP"
BASE COUNT          291 a          942 c          932 g          266 t
ORIGIN      
        1 cgctgttcgc cggcctctcc gaggcggcgg cggggcggga ggaccggctc gccaaccgcc
       61 ccgccgggct ggcctacgcc ctgcgccggc tcggcaccgg ggcccaggag ccgctgtggg
      121 ggcggctggc cggcctgcgc ccgccggccc tgctggtggc gggggcgctg gacgccaagt
      181 tcgccggcat cgcccgcgag atggccgccg ccatcggccc cgcggcgcgg gtcgcgctcg
      241 tccccggggc cgggcacgcc gtccacctgg aacgcccgga cgccatggcc gctctgctcg
      301 aggcattcct cgacgacccc ggggcccggc cctgaccgcc ggctcatgac caccacagcc
      361 cggccgtgag cagcaggctg accgcgagct ggagcctggc cgtggcccca agggcggcga
      421 tcaggccggg cccgtcggtg cgggtccgga ccagccggac cggggccacg gccaggggga
      481 cggcggccag ggtgaccagg accggcggcc gggccagccc gagcagggcg gcgacgccga
      541 acatgcccgc gaccacggcc gtgaacaggt cgcgggcccg gtcgcggccg aggcggacgg
      601 ccagggtgcg cttgccggcc aggcggtcgc cgtcgacgtc gcggacgttg ttggccagca
      661 ggatcgccac cgcgcccagc cccacgacca gcgacgcggc cgcggccgcg gccggcaccc
      721 gctccagctg cacgtaggcc gacccgcagg tcgcgaccac cccgaagaag gccagcacgg
      781 ccagctcgcc gaagccggcg tagccatacg gccgcggccc gccggtgtac aggacggcgg
      841 ccgcgatcgc cgccaccccg accagcagca gccggaggtc gacggccacg gccagggcca
      901 ggccggccag ggcggccacg gcgaaggcga ccccggcggc ccggcgcacg gccgccgggg
      961 cggccaggcc ggcggcggtg aggcgggccg ggccgagccg gtccggggtg tcggcgcccc
     1021 gcaccccgtc ggagtagtcg ttggcgtagt tgaccccgat ctgcagggcc acggccacgg
     1081 ccagggcggc cagcgcccgc caggccacca ccggtccctc cgaggcggcc gcggccgtcc
     1141 ccaccaggac cgggcacacg gccgcgacca gcgtcctcgg ccgcgccccc tgcacccaga
     1201 cgttcacggg ccccgggtcc ctagggccgc ttggggaact tcgcgaagtc cggagggcgc
     1261 ttctcccgga aggcgttgcg gccctcctgg gcctcggagg tcatgtagta gaggagggtg
     1321 gcgtcgccgg ccagctgctg gaccccggcc aggccgtcgg tgtcggcgtt gagcccggcc
     1381 ttgagcagcc gcagggccag cgggctgtgc tccaggatac ggcggcacca ctcgaccgtg
     1441 acccgctcca ggtcggccag ggggacgacg gtgttgacca ggcccatctc cagggcctgg
     1501 gccgcgtcgt actggcggca gagcagccag acctcggccg ccttcttgcg cccgacggtg
     1561 cgggccagca tgcccatgcc gtagccgccg tcgaaggacc cgacccgcgg cccggtctgg
     1621 ccgaagcggg cgttgtcggc ggcgatggtc aggtcgcaga ccaggtggag cacgtggccg
     1681 ccgccgatgg cgtagccggc caccatggcg atcaccggct tgggcagccg ccggatctgg
     1741 atctggaggt cgagcacgtt gaggcggccg atgccgcgct cgtcgacgta gccgtcgtcg
     1801 ccgcggatgc gctggtcgcc gccggagcag aaggcgtccg ggccctcgcc ggtgaggacg
     1861 atcaccccga tcgacgggtc gtcgcgggcg agctcgaagg cgtcctggag ctcgaacagg
     1921 gtcttgggac ggaaggcgtt gcggacctcc ggccggcgga tggtgatctt ggcgatcccc
     1981 tccgcggtct cgtagccgat gtcggtccag ctgccctggt gctgccactc cacgggcgta
     2041 tcagctcctc ggacatcgcg gttcggtggc gacggccctg ctagcctacc ggcccatgtc
     2101 gtcctctgtt gccctggtcg ccgtcgccct gccccggccg gccgccgccc gggcgatcgt
     2161 ggccgcctgg gaggccggcg aggccgtcct gcccctcgac ccggacgccc cggcgcccga
     2221 gctgaagggc atcctggccg cggcccgccc cacccacctg ctggacacgg acgggcgcac
     2281 ccggctgccc gagggggcgc cggccgagcc cggcctggcc gcggtggtgg ccacctccgg
     2341 caccagcggc gcccccaaga tggtcgagct gggcgccgac gcggtccgct ggtcggccct
     2401 ggccaccacg gccgccctgg aggccgcgcc g
//
LOCUS       DASSWR010000260         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_236136,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000260 DASSWR010000000
VERSION     DASSWR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236136"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFO47_04395"
     CDS             <1..960
                     /locus_tag="VFO47_04395"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HET8787967.1"
                     /translation="MAGRVAGALARRGLAVHRLEVPAGEAAKRLEVVAGLYERLAAVP
                     TRRADPVVAVGGGATTDTAGFAAATWLRGVPLVNVPTTVLGMVDAAVGGKTGVDLDAG
                     KNLVGAFHQPVAVVADLDTLAGLPAAEVRSGLAEVAKAGLAGDPALAEALARSAGPAV
                     AGDPVALAPLVEGAVRVKAAVVGADEHEEGGDGRVGRLMLNYGHTLGHALERLAGYRG
                     LRHGEAVALGMVFAARVAEAVGLARPGLAAGHVELLAALGLPVGGVELDPDRVLAAMA
                     TDKKHRGGLRLVLLRRLGEPEVVPAPDRRVLVEAIRSLAEVPR"
     gene            957..1430
                     /locus_tag="VFO47_04400"
     CDS             957..1430
                     /locus_tag="VFO47_04400"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET8787968.1"
                     /translation="MKAYPRTGQGPLVLVLHGPNLSSLGRRDPGHYGTLDLDQIMERL
                     SKLAGELGLSVEGLQSDHEGDLVEALLAAAGRGVAAAVCNPGALAHTSYAVRDAVEAA
                     GLPVVEVHLSNVHARERWRHHLAVAPVAAAVIAGMGPASYDLGLRAAADLLARRA"
     gene            1471..2559
                     /locus_tag="VFO47_04405"
     CDS             1471..2559
                     /locus_tag="VFO47_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET8787969.1"
                     /translation="MPNDGHAYSEARRQALRGLLAHADLSALLVTKPVNVRYLTGFTG
                     SYGALLLLHDRTLFFTDGRYRHQAAAEVPGAEVVPAPGDLIGAVAGVVRTAGNGLGFE
                     PGGLTWGEGQRLRSALPGQAVVPAPPLVEELREVKDERELAAMREAARLGGEALEGLL
                     PALREGMTERQVAVDLELAMRRLGADGLAFESIVAFGEQAAEPHHHPGGRPLRAGDLV
                     KVDFGARVDGYCADMTRTVVLGKASQRQRDLYELVRAAQAAGLACLEAGVPAGEVDRA
                     CRQPIAEAGLGDSFPHPTGHGIGLEIHEAPRLRAGSRDRVAADTPVTVEPGVYLPGFG
                     GVRIEDLVVARPGGHELLTTVPKELLEL"
     gene            2556..>2964
                     /locus_tag="VFO47_04410"
     CDS             2556..>2964
                     /locus_tag="VFO47_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET8787970.1"
                     /translation="MNQETLGELAEEYFQVLMDASPFDATMFGVPGWDAEVPDLSEDG
                     DAETDGRLAGLERRLAALDPAGLGPQDRITLAMLGRGIADRRDELAARWPELTVSATS
                     RGIQTAVLGLVPKVALATGEQAADYTERCARLAG"
BASE COUNT          319 a         1133 c         1158 g          354 t
ORIGIN      
        1 atggccgggc gggtcgccgg cgccctggcc cggcgtggcc tggccgtcca ccggctggag
       61 gtgccggcgg gggaggcggc caagcggctg gaggtggtgg ccgggctgta cgagcggctg
      121 gccgcggtcc cgacccgccg ggccgacccg gtggtggccg tcggcggcgg cgccaccacc
      181 gacaccgccg ggttcgcggc cgccacctgg ctgcgcgggg tgccgctggt caacgtgccc
      241 acgaccgtgc tcggcatggt cgacgccgcc gtcgggggca agaccggggt cgacctggac
      301 gccggcaaga acctggtcgg cgccttccac cagccggtgg ccgtggtggc cgacctggac
      361 accctggccg gcctgccggc ggccgaggtc cgctccggcc tggccgaggt ggccaaggcc
      421 gggctggccg gcgaccccgc cctggccgag gccctggccc gctccgccgg gccggccgtg
      481 gccggcgacc cggtggccct cgcccccctg gtcgaggggg cggtccgggt caaggcggcc
      541 gtggtcggcg ccgacgagca cgaggagggc ggcgacggcc gggtcggccg cctgatgctg
      601 aactacgggc acaccctggg ccacgccctg gagcggctgg ccgggtaccg ggggctgcgg
      661 cacggggagg cggtggcgct ggggatggtg ttcgcggccc gggtggccga ggcggtcggg
      721 ctggcccggc cggggctggc cgccgggcac gtcgagctgc tggccgccct cggcctgcct
      781 gtcggcgggg tcgagctcga cccggaccgg gtcctggccg ccatggccac cgacaagaag
      841 caccgcggcg gcctgcgcct ggtcctgctg cgccgccttg gtgagcccga ggtcgtcccc
      901 gcccccgacc gccgggtcct ggtcgaggcc atccgttccc tggccgaggt gccgcgatga
      961 aggcctatcc gaggacgggg caggggccgc tggtgctggt gctgcacggg cccaatctgt
     1021 cctcgctggg gcggcgcgac cccgggcact acggcaccct ggacctggac cagatcatgg
     1081 agcggctttc caagctggcc ggcgagctcg gcctgtccgt tgagggcctc cagtccgacc
     1141 acgagggcga tctggtcgag gcgctgctgg ccgcggccgg tcgcggggtg gccgccgccg
     1201 tctgcaaccc gggggcgctc gcccacacct cgtacgcggt ccgcgacgcc gtcgaggccg
     1261 ccggcctccc ggtcgtggag gtccacctgt cgaacgtcca cgcccgcgag cgctggcgcc
     1321 accacctggc cgtggccccg gtcgcggccg cggtgatcgc cggcatgggc ccggccagct
     1381 acgacctggg cctgcgcgcc gccgccgacc tgctggctcg ccgcgcctga gccgggttgg
     1441 caagatggac cgggggaaag gaacgctcgc gtgcccaacg acggccatgc ctacagcgaa
     1501 gccaggcggc aggccctgcg cggcctgctg gcccatgccg acctgtcggc cctgctggtc
     1561 accaagccgg tcaacgtccg ctacctgacc gggttcaccg gcagctacgg ggcgctgctg
     1621 ctgctgcacg accgcaccct gttcttcacc gacggccgct accgccacca ggccgccgcc
     1681 gaggtccccg gggccgaggt cgtgcccgcc cccggcgacc tgatcggggc cgtcgccggg
     1741 gtggtgcgca ccgccggcaa cggcctcggg ttcgagccgg gcgggctgac ctggggcgag
     1801 ggccagcggc tgcgctcggc cctgcccggc caggccgtcg tgcccgcccc gcccctggtc
     1861 gaggagctgc gcgaggtcaa ggacgagcgc gagctggccg ccatgcgcga ggcggcccgg
     1921 ctcggcggcg aggccctcga ggggctgctg ccggccctgc gcgagggcat gaccgagcgg
     1981 caggtggccg tcgacctgga gctggccatg cgccgcctcg gcgccgacgg gctcgccttc
     2041 gagtccatcg tcgccttcgg ggagcaggcg gccgagcccc accaccaccc cggcggccgg
     2101 ccgctgcggg ctggcgacct ggtcaaggtc gacttcggag cccgggtcga cggctactgc
     2161 gccgacatga cccgcacggt cgtgctcggc aaggcctccc agcgccagcg tgacctgtac
     2221 gagctggtcc gcgccgccca ggcggccggg ctggcgtgcc tggaggcggg ggtgccggcc
     2281 ggcgaggtcg accgggcctg ccgccagccg atcgccgagg ccgggctggg agactcgttc
     2341 ccgcacccga ccggccacgg catcggcctg gagatccacg aggcgccgcg gctgcgggcc
     2401 ggctcccggg accgggtggc cgccgacacc ccggtcaccg tcgagcccgg ggtgtacctt
     2461 cccggcttcg gcggggtccg gatcgaggac ctggtggtgg cccgccccgg cggccacgag
     2521 ctgctcacca ccgtccccaa ggagctgctg gagctgtgaa ccaggagacg ctcggcgagc
     2581 tggccgagga gtacttccag gtgctcatgg acgcctcgcc gttcgacgcc accatgttcg
     2641 gggtgcccgg ctgggacgcc gaggtccccg acctgtccga ggacggcgac gccgagaccg
     2701 acgggcgcct ggccggcctg gagcgccgcc tggccgccct cgacccggcc gggctcggcc
     2761 cccaggaccg gatcaccctg gccatgctcg gccgcgggat cgccgaccgc cgggacgagc
     2821 tggccgcccg ctggcccgag ctgaccgtgt cggccaccag ccggggcatc cagacggccg
     2881 tgctcggcct ggtccccaag gtcgccctgg ccaccggtga gcaggccgcc gactacaccg
     2941 agcgctgcgc ccggctggcc ggct
//
LOCUS       DASSWR010000261         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1081992, whole genome shotgun sequence.
ACCESSION   DASSWR010000261 DASSWR010000000
VERSION     DASSWR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081992"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFO47_04415"
     CDS             complement(<1..328)
                     /locus_tag="VFO47_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787971.1"
                     /translation="MRWLAGLRLPGLTGLMEAIVASTGSVGMVGALRWATVVALLVLR
                     RFRHLVVFVGSFLAVLLVVRLATVDRPRPFGVELRGSWAGWTMPSRPVAILAATLVGV
                     LYTLVPV"
     gene            complement(676..1971)
                     /locus_tag="VFO47_04420"
     CDS             complement(676..1971)
                     /locus_tag="VFO47_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET8787972.1"
                     /translation="MAAASPAGVTAATPAELTAICEDLFAGVYETPSSEPLAVCQWDM
                     ALIDANASAWSNATGAGVTIGILDSGVDPTHPDIAPNLDLARSCSFISPGTPTADPVE
                     VATSCDNKAAVRDLYGHGTHVASIAAAPVNGIGIAGVAPEATLVALKACTAGGFCFVD
                     SVAAALRYAGDQRLDVVNMSLFADPYLYYCRNEAEQRAMLKELQSAARYAQQRGVLLV
                     ASAGNEADDLQHPTIDEISPDYPPDSAETRRVRNNCRVAPAELPGVVTVSATGPVGYP
                     GYTMNIAEYSSVGMGVVDVTAPGGDYFSATDTVQDAVLGALPNTPSVLWDAFDPLEPD
                     LPGLTVVDQGARYAFLNGTSMAAPHAAGVAALIRQVHPEWSQGAVAAALERSATPLAC
                     PVDWQPINEDDDRQRCYGSANRNSFFGAGIVNAARAAAL"
     gene            complement(2105..3073)
                     /locus_tag="VFO47_04425"
     CDS             complement(2105..3073)
                     /locus_tag="VFO47_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HET8787973.1"
                     /translation="MTPAEIIYHRRVQVLDRAGQTSVTEACRTFGVSRTTYYRWAGRA
                     QRYGLAALLPKGRRPPVMPTATPPDQVEAVLAEAVARPTLGARQLVCHLADRGVRLSP
                     SGVQKILGRHRLGRRAQRVAALAQLTAATTGILTSVAKDGPFGFCHFAARPGDLVALD
                     TFYVGKLKGIGPVWQLTAVDTATRYAIATLVAGDKSARDAAAFVDHVAERLAGIGAEL
                     TGVLTDNGPEFTGHQFTAARAERGLRHHRIPPRSPNHNAVCERFQGTALQEFYRPAFH
                     RQHFTRLADLGAQFQGWLQHYNTRRRNHGDFMRGRTPFAAMEAHLS"
BASE COUNT          486 a         1087 c         1237 g          442 t
ORIGIN      
        1 ccaccggcac cagtgtgtag agcaccccga ccagggtggc ggccaggatc gcgaccggcc
       61 gggacggcat tgtccagccg gcccagcttc cccgcagctc caccccgaac ggccgcgggc
      121 gatccaccgt cgccaaccgg accaccagca ggacggccag gaacgagccc acgaacacaa
      181 ccaggtggcg gaagcgccgc aacaccagca atgcgaccac ggtcgcccag cgcagcgccc
      241 cgaccatgcc gaccgatccg gtcgaggcga caatcgcctc catcaagccg gtcaggccgg
      301 gaagccgcag cccggccagc cagcgcacca ccgcgtcatc ccacgccgtc acggccacgc
      361 cgaggctgcg ccgggccgga ccgaaggcga tcctggccag cgcgacgagg accacggcgg
      421 cggccgccca ccagacgccg gtgggctgga tactgcgcgg cagcggaggc ggggcgccgg
      481 tgggacgccg tcggcgcccc gaccgccgta cctccggttg gcccggtctc ggcggtccaa
      541 cggcgcccgg gagcaccgga cgtggggtgg agtccatctg cccatcctgc aaccaggcga
      601 cctgccagtc caccgcatgg cgtggacggg ttggagcccc ggctgggccg gggctccaac
      661 agtgtccagc ttcgactaca gcgcggccgc tcgggcggcg ttcacgatgc cggcgccgaa
      721 gaaggagttg cggttggcgc tgccatagca gcgttggcgg tcatcgtcct cgttgatcgg
      781 ctgccagtcg accgggcagg cgaggggggt ggccgagcgt tccagcgcgg ccgcgaccgc
      841 cccctgcgac cactccgggt ggacctggcg gatcagggcg gcgaccccag cggcatgggg
      901 ggccgccatc gaggtgccgt tcaggaaggc atagcgggcg ccctggtcga ccacggtgag
      961 gccgggcagg tcgggctcca gcgggtcgaa ggcgtcccag agaaccgacg gcgtgttcgg
     1021 gagcgccccg agcacggcgt cctggacggt gtcggtggct gagaagtagt cgccgccggg
     1081 agcggtcacg tcgaccacgc ccatgccgac cgaggagtac tcggcgatgt tcatggtgta
     1141 gccggggtag ccgaccggac cggtggccga caccgtcacc acgcccggca gctcggccgg
     1201 agcgacccgg cagttgttgc ggacccggcg ggtctcggcg ctgtcgggcg gatagtcggg
     1261 gctgatctcg tcgatcgtcg ggtgctggag gtcgtcggcc tcgttcccgg ccgaggcgac
     1321 cagcaggacg ccgcgctgct gggcgtagcg ggcggccgac tggagctcct tgagcatcgc
     1381 ccgctgctcg gcctcgttgc ggcagtagta gaggtagggg tcggcgaaca ggctcatgtt
     1441 cacgacatcc agccgctggt cgccggcata gcgcagggcg gccgcgaccg agtcgacaaa
     1501 gcagaagccc ccggccgtgc aggccttgag ggcgaccagg gtcgcctcgg gggccacgcc
     1561 ggcgatcccg atgccgttga cgggggcggc cgcgatggag gccacgtggg tgccgtggcc
     1621 gtacaggtcg cggaccgcgg ccttgttgtc gcagctggtg gccacctcca ccggatcggc
     1681 ggtcggcgtg cccgggctga tgaacgagca ggagcgggcc agatccaggt tgggcgcgat
     1741 gtcggggtgg gtcggatcga caccgctgtc gaggatgccg atggtcacgc ccgcaccggt
     1801 ggcattggac caggccgagg cgttggcgtc gatcagcgcc atgtcccact ggcagacggc
     1861 gagcggttcg ctgctcgggg tctcgtagac gccggcgaac aggtcttcgc agatggcggt
     1921 cagctccgcc ggcgtggccg cggtcactcc ggccggcgat gcggcggcca tcgaggccag
     1981 ccccgacaga cagagcacgg ccgccgtcat gagtacgagc aatgcccgac gtaggtgcat
     2041 gatgagcacc ctccctaggt cttcctgggc acgggttgag gtgacaggtg gaccctcggc
     2101 ttggtcatga taggtgcgcc tccatggccg caaagggcgt gcggccgcgc atgaagtcgc
     2161 cgtggttgcg gcggcgggtg ttgtagtgct gcagccagcc ttggaactgg gcgcccaggt
     2221 cggccaggcg agtgaagtgc tgtcggtgga aggccggccg gtagaactcc tggagcgcgg
     2281 tcccttggaa gcgttcgcag accgcgttgt ggttgggcga gcggggcggg atccggtggt
     2341 gccgcaggcc gcgttcggcc ctggcggcgg tgaactggtg tccggtgaac tctgggccgt
     2401 tgtcggtgag gaccccggtg agctcggcgc cgatgccggc caggcgctcg gcgacgtggt
     2461 cgacgaaggc cgcagcgtcc cgggccgact tgtcaccggc caccagcgtg gcgatcgcat
     2521 accgggtggc ggtgtcgacc gccgtcagct gccagaccgg cccgatgccc ttgagtttgc
     2581 cgacatagaa ggtgtccaac gcgacgagat cgccaggacg ggccgcgaag tggcagaacc
     2641 cgaacgggcc gtccttggcc acgctggtga ggatgccggt cgtcgcggcg gtcagctggg
     2701 ccagcgcggc aacgcgctgg gcacgtcggc cgagccggtg gcgacccagg atcttctgca
     2761 cgcccgacgg cgagaggcgg accccacggt cggcgaggtg gcagaccagc tggcgggcgc
     2821 cgagggtcgg ccgggcgacc gcctcggcca gcaccgcctc gacctggtcg ggtggggtcg
     2881 cggtcggcat caccggcggt cggcggccct tgggcagcag cgccgccagc ccatagcgtt
     2941 gggcgcggcc ggcccagcgg tagtaggtgg tccgcgagac gccgaaggtg cggcaggcct
     3001 cggtcacgct ggtctggccg gcccggtcca ggacctggac acgccggtgg tagatgatct
     3061 ctgctggggt cacggtggtg gactcctgcg gctctttggt cggaaccaaa gggtccacca
     3121 ccagccccac cagcagtggg acctgcaacc tcaacccgtg atcagcaagc cctagaggtc
     3181 acgttgagct acaacctggt gatggcgtgt cggtcgctgc tgaaccgcat cctgcaggcc
     3241 gccgtcgatg aa
//
LOCUS       DASSWR010000262         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_8539,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000262 DASSWR010000000
VERSION     DASSWR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8539"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="VFO47_04430"
     CDS             complement(<1..1531)
                     /locus_tag="VFO47_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HET8787974.1"
                     /translation="MADIAEVDLTAGRGPLDPEELDRIDAWWRAANYLSVGQIYLLDN
                     PLLREPLRPEHVKPRLLGHWGTTPGLNFVYVHMNRVIKREDLDAIYVTGPGHGGPGLV
                     ANVYLEGTYSEVYPHIGQNEEGLRRLFRQFSFPGGVPSHVAPETPGSINEGGELGYSL
                     VHAYGAAFDHPDLFVCCVVGDGEAETGPLAASWHSNKFLNPISDGAVLPILHLNGYKI
                     ANPTILARIPEAELRALLEGYGYAPRFVSGSEPEDMHQRMAATLDEVVTEIHEIQRHA
                     RAEDDPTRPRWPMIVLRTPKGWTGPEEVDGQQVEGTFRSHQVPLAGLAQRPDHLAALE
                     DWLRSYRPEELFDATGAFRAELATLAPTGERRMGANPAANGGRLLRQLELPDFRAYAV
                     DVPEPGAATAEATRVLGGYLRDVIAANPTTFRLMGPDETSSNRLNAVFEVTDRTWVAE
                     PGPGDEDLSPDGRVMEVLSEHLCQGWLEGYGYAPRFVSGSEPEDMHQRMAATLDEVVT
                     EI"
     gene            1783..>2074
                     /locus_tag="VFO47_04435"
     CDS             1783..>2074
                     /locus_tag="VFO47_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET8787975.1"
                     /translation="MNRPAASSSLGAGPPAAAPPADPSLLDAAEVARSLGSDPASGLS
                     STEAAERLARAGPNRLDPAAQVPTWRKLLAQFADPLIYLLLAAITISVVAWAL"
BASE COUNT          270 a          751 c          752 g          301 t
ORIGIN      
        1 ggatctcggt cacgacctcg tccagcgtcg ccgccatccg ctggtgcatg tcctctggct
       61 cggacccgct cacgaagcgt ggcgcgtagc cgtagccctc cagccagccc tggcacaggt
      121 gctcggagag gacctccatc acccgcccgt ccggggacag gtcctcgtcg cccggccccg
      181 gctcggcgac ccaggtccgg tcggtgacct cgaacaccgc gttgaggcgg ttggagctgg
      241 tctcgtcggg gcccatcagg cggaaggtgg tcgggttggc ggcgatcacg tcgcgcaggt
      301 agccgcccag gacgcgggtg gcctcggccg tggccgcgcc gggctcgggc acgtcgacag
      361 cgtaggcgcg gaagtcgggc agctcgagct ggcgcagcag gcggccgccg ttcgcggccg
      421 ggttggcccc catgcgccgc tcaccggtcg gcgcgagggt ggccagctcg gcccggaagg
      481 ccccggtcgc gtcgaacagc tcctcgggcc ggtaggagcg caaccagtcc tccagggcgg
      541 ccaggtggtc gggccgctgg gccaggccgg ccagcggcac ctggtgggac cggaaggtcc
      601 cctcgacctg ctggccgtcg acctcctccg ggccggtcca gcccttgggg gtgcgcagga
      661 cgatcatcgg ccagcggggc cgggtggggt cgtcctcggc gcgggcgtga cgctggatct
      721 cgtggatctc ggtcacgacc tcgtccagcg tcgccgccat ccgctggtgc atgtcctccg
      781 gctcggaccc gctcacgaag cgcggcgcgt agccgtagcc ctccagcagc gcccgcagct
      841 cggcctcggg gatgcgggcc aggatcgtgg ggttggcgat cttgtagccg ttcaggtgca
      901 ggatcgggag gacggcaccg tcgctgatcg ggttgaggaa cttgttcgag tgccagctgg
      961 ccgccagcgg gcccgtctcg gcctccccgt cgccgaccac gcagcagacg aacaggtcgg
     1021 ggtggtcgaa ggcggcgccg taggcgtgca ccagggagta gccgagctcg ccgccctcgt
     1081 tgatcgaccc gggcgtctcg ggcgccacgt ggctggggac gccgcccgga aaggagaact
     1141 ggcggaacag ccgccgcagc ccctcctcgt tctgaccgat gtgcgggtag acctcgctgt
     1201 aggtgccctc caggtagacg ttcgccacca gccccgggcc accgtggccg ggcccggtca
     1261 cgtagatggc gtccaggtcc tcccgcttga tgacccggtt catgtgcacg tagacaaagt
     1321 tcagcccggg ggtcgtgccc cagtggccca gcaggcgtgg cttgacgtgc tcggggcgca
     1381 gcggctcgcg cagcagcggg ttgtcgagca ggtagatctg gcccacggac aggtagttgg
     1441 cggcccgcca ccaggcgtcg atgcgatcaa gctcctcggg gtccagcgga ccccggccgg
     1501 ccgtcaggtc gacttcggcg atatcggcca tggcaacacc cgtgatccta gcgacggact
     1561 cccttcctcc cgccctgagt atgaccatcg aaggggcccg tattcacccg gccgtcagcc
     1621 gacgacggtg ttgacgaggc ggccgagccc gctgtttccg gccgccttca ccgatgcacc
     1681 ctttcgccag taaggtcgaa gcaccaccat gcgcccacgg gcaagggaga cgaacatgtg
     1741 gcgtgcaggc gccggaggct gcctccgcgg ccgcggaggt cgatgaaccg gccggccgct
     1801 tcctcctcgc tgggggcggg cccgccggcg gccgccccgc cggccgaccc gtcgctgctg
     1861 gacgcggccg aggtggccag gtcgctgggc agcgacccgg cctcgggcct cagctcgacg
     1921 gaggccgccg aacgcctggc ccgcgccggc cccaaccgcc tcgaccccgc cgcccaggtg
     1981 ccgacctggc gcaagctgct cgcccagttc gccgacccgc tgatctacct gctgctggcc
     2041 gccatcacga tctcggtggt cgcctgggcg ctgg
//
LOCUS       DASSWR010000263         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_179931,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000263 DASSWR010000000
VERSION     DASSWR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179931"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..953)
                     /locus_tag="VFO47_04440"
     CDS             complement(165..953)
                     /locus_tag="VFO47_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET8787976.1"
                     /translation="MARLDHDRCCDEVVTQTTLLRDTVSGADLSQVVPTTPDWTLGDL
                     ARHVGGNLLAGEQAVRTGTAVTEPERQVPGHGGPAGDDPAALDAWLAEAAERFAETLR
                     KAGPATEAQIWVVRWPTAAWARRAAHDVVVHRADAAGAVGAAYLVDPEVAADAVDEFL
                     DLLSNPQVAGSGDGGGDAGLRGTVHLHATDTPPGLASEWLVELEPPTFSWRLAHEKAT
                     VAVRAPVADVLRVVTRRLPPGDDDVEILGDRALLDAWLERLVLR"
     gene            1007..2671
                     /locus_tag="VFO47_04445"
     CDS             1007..2671
                     /locus_tag="VFO47_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8787977.1"
                     /translation="MEASKPVLLTVDDDPGVSRAVARDLRRRYGQDYRVVRAESGRHA
                     LEALAEIKLRGDPVAVLLADYRMPEMSGLEFLEQAMDLFPNARRALLTAYADTDAAIQ
                     AINVIDLDHYLLKPWDPPDEKLYPVVDELIELWRDTGDQQAGATRVVGHRWSARSYEV
                     RDFLARNSVAYRWLTADDPEGRRLLEAAGLDEAGVPLVVTPEGDVLVDPGVEELAARV
                     GLPTTPATGFYDTVIVGGGPAGLGAAVYAASEGLRTVLVEREATGGQAGQSARIENYL
                     GFPDGVSGAQLTDRARRQAGKFGAEVLTAATVTGLEARGSSRVVRFADGGEVAAHTVV
                     LATGVSYHTLKVPGVAELTGRGIFYGSAATEAPACADEDVYVVGGANSAGQAAVFLSR
                     HARRVTLLVRADGLERSMSHYLIRQIRDTANIQVRTNTEVAGVEGTEHLEGLRLCDAA
                     TGEVEEVPAAFLFVFIGAAPCTDWLDKVVERDARGFVVTGPDLLRGGRRPRGWPLDRD
                     PYYLEGSVPGVFAAGDARANSVKRVASAVGEGAMAIQLVHRYLEAQ"
     gene            2668..>3132
                     /locus_tag="VFO47_04450"
     CDS             2668..>3132
                     /locus_tag="VFO47_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8787978.1"
                     /translation="MTERLAPAALRELFLFERLSDDQLAWLAERGEVAAYEAGTTIYR
                     VGEPAASLYVLLEGTLSLVMRAPGGEIEVNRTDHRGTYAGAFFAYLDLPMARSYGTGL
                     RAVTDCRFWVLSADDFGRAVREWFPMATHMLEGFAIQGMGSQQTLSTRERLVA"
BASE COUNT          380 a         1194 c         1165 g          393 t
ORIGIN      
        1 ccgggccggc ggccgagtgc ctgatcgacc gcagcccgcc gccaccgccc cgcgaccctc
       61 aggcgtggcc ggtccgccgc gaccccggca tgggccgccc gaccaagcgc gaccggcgcc
      121 agctcgaccg cacccggggc cgccgcggct gacccgccga cgggtcagcg aaggacgagc
      181 cgctccagcc aggcgtcgag cagggcccgg tcgccgagga tctcgacgtc gtcgtcgcct
      241 ggcggcaggc ggcgggtcac gacccggagc acgtcggcga ccggggcgcg gacggccacg
      301 gtcgccttct cgtgggccag gcgccagctg aacgtggggg gctccagctc gaccagccac
      361 tccgacgcca gcccgggagg cgtgtcggtg gcatgcaggt gcacggtccc gcggagtccg
      421 gcgtccccgc cgccgtcccc ggacccggcg acctggggat tgctcagcag gtcgaggaac
      481 tcgtcgacgg cgtcggcggc cacctccggg tcgaccaggt aggcggcgcc gaccgccccg
      541 gcggcgtcgg cgcggtggac gaccacgtcg tgggcggcgc ggcgggccca ggcggcggtg
      601 ggccagcgga ccacccagat ctgggcctcg gtggcgggcc cggccttgcg cagcgtctcg
      661 gcgaaccgct cggccgcctc ggccagccag gcgtccagcg ccgccgggtc gtcgccggcc
      721 gggccgccgt ggcccgggac ctgccgctcc ggctcggtga cggccgtgcc ggtgcggacg
      781 gcctgctcgc ctgccaggag gttgccgccg acgtggcggg ccaggtcgcc cagggtccag
      841 tcgggcgtgg tgggcaccac ctgcgacagg tcggcgcccg agaccgtgtc cctgagcagc
      901 gtggtctggg tgacgacctc gtcgcagcag cggtcgtggt ccaggcgcgc catgcgttcc
      961 cccgtgcggt tgttgctgtc cggccccgac cctaccatcg ggcaccatgg aggccagcaa
     1021 accggtcctg ctcacggtcg acgacgaccc cggggtgtcg cgcgccgtcg cccgcgacct
     1081 gcgccgccgc tacgggcagg actaccgcgt cgtccgggcc gagtcgggcc gccacgcgct
     1141 ggaggcgctg gccgagatca agctccgcgg cgatccggtg gcggtgctgc tggccgacta
     1201 ccggatgccg gagatgagcg gcctggagtt cctggagcag gccatggacc tgttcccgaa
     1261 cgcccggcgg gcgttgctca ccgcctacgc cgacaccgac gccgccatcc aggcgatcaa
     1321 cgtgatcgac ctcgaccact atctgctgaa gccgtgggac ccccccgacg agaagctcta
     1381 tccggtggtg gacgagctga tcgagctgtg gcgcgacacc ggggaccagc aggccggcgc
     1441 gacccgggtc gtggggcacc gctggtcggc gcggtcctac gaggtgcggg acttcctggc
     1501 ccgcaactcg gtggcgtacc gctggctcac cgccgacgac cccgagggcc ggcggctgct
     1561 cgaggcggcc gggctcgacg aggccggcgt ccccctggtc gtcacccccg agggcgacgt
     1621 cctggtcgac ccgggcgtgg aggagctggc ggcccgggtc gggctgccga ccaccccggc
     1681 caccggcttc tacgacaccg tcatcgtcgg cggcggcccc gccgggctgg gcgcggccgt
     1741 gtacgccgcc tccgaggggc tgcgaaccgt cctggtcgag cgggaggcca ccggcgggca
     1801 ggccggccag agcgcccgga tcgagaacta cctcggcttc cccgacgggg tcagcggcgc
     1861 ccagctcacc gaccgggccc gccgccaggc cggcaagttc ggggccgagg tcctcaccgc
     1921 cgccaccgtc accggcctgg aggcccgcgg ctcatcccgg gtggtccgct tcgccgacgg
     1981 cggcgaggtc gccgcccaca ccgtggtcct ggccaccggg gtcagctacc acaccctgaa
     2041 ggtgcccggc gtggccgagc tcaccggccg cgggatcttc tacgggtcgg ccgcgaccga
     2101 ggccccggcc tgcgccgacg aggacgtgta cgtcgtcggg ggcgccaact ccgccggcca
     2161 ggcggcggtg ttcctgtccc ggcacgcccg gcgggtcacc ctgctggtcc gcgccgacgg
     2221 cctggagcgc tccatgtccc actacctcat ccgccagatc cgtgacaccg ccaacatcca
     2281 ggtacggacc aacaccgagg tcgccggagt ggaggggacc gagcacctgg agggcctgcg
     2341 actctgcgac gccgccaccg gcgaggtcga ggaggtgccg gcggccttcc tgttcgtgtt
     2401 catcggagcg gcgccctgca ccgactggct cgacaaggtg gtggagcgcg acgccagggg
     2461 cttcgtcgtc accggcccgg acctgcttcg cggcggccgg cggccgcgcg gctggccgct
     2521 ggaccgcgac ccctactacc tggagggcag cgtcccgggc gtgttcgccg ccggcgacgc
     2581 ccgcgccaac tcggtgaagc gggtcgcctc ggcggtgggg gagggcgcca tggccatcca
     2641 gctcgtccac cgctacctgg aggcccagtg accgagcgcc tcgctcccgc cgccctccgc
     2701 gagctgttcc tgttcgaacg gttgagcgac gaccagctgg cctggctggc cgagcggggc
     2761 gaggtcgccg cctacgaggc cggcaccacc atctaccgcg tcggcgagcc cgcggccagc
     2821 ctgtacgtgc tcctggaggg caccctgtcg ctggtcatgc gggcccccgg cggcgagatc
     2881 gaggtgaacc ggaccgacca ccgcggcacc tacgccgggg cgttctttgc ctacctcgac
     2941 ctgccgatgg cgcgctccta cggcaccggg ctgcgtgccg tgaccgactg ccgcttctgg
     3001 gtgctgtccg ccgacgactt cggccgggcg gtgcgggagt ggttcccgat ggccacgcac
     3061 atgctggagg ggttcgccat ccagggcatg ggctcccagc agacgctcag cacccgcgag
     3121 cggctggtcg cc
//
LOCUS       DASSWR010000264         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_518732,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000264 DASSWR010000000
VERSION     DASSWR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518732"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2295)
                     /locus_tag="VFO47_04455"
     CDS             complement(<1..>2295)
                     /locus_tag="VFO47_04455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET8787979.1"
                     /translation="VDQAGLILQAGDLLEDHPDILAATVAQVGTILVDEAQELDPAQR
                     RLLTLLVDGGTRAILAGDPAGATDTFRGATPGALPALAKDLGASVVLLERSRRLGGPG
                     LDAVRRLQAAGPGPVVALTGGGTTIVEAARYPGPAEEAEANARLLKVAHERDGVPYGR
                     MAVLLPSTRRLSAPTRRALERFGVPYRLGAGERQLVAEPVVGHVLDLFRLALDPARAD
                     ELLPTLLTSPLGGLDPGELRALRRAALLADRSLADHVRARAVPPPPAEPDLDPADREG
                     GGRDTPGPEAVGPEEAEAAEASTADGDGGEVGPARTGSALVLDPGLVARVQALCDLLE
                     GAERWVAELDADACFWEVWRTAPAFSELILRAEVDPTDADVQRQLDALTAFSRALGLF
                     VDGRPGASMRTYLDVVERADFASDPWLPPGMTRNDTVAVLSVNAAKGQEFDLVIVAGC
                     LEGALPMTARPQGLFEAWRLDGDPGPVVRANELLDAERRRFTLAASRARDRVVFTASR
                     VDGRGEPSRFLRELGLELPADPPAPETAPLGSLEAAGALRRVAGDRDRPAAERLAAAA
                     TLAAIPGVDPATWWWRRDYTVDPEPIAAEGRLRTSYSRIGTYEDCHLKYFFGSVAGLD
                     DRSSYQMAFGRLMHTIFELAAKGEITNQPEDLKAAYRERFNPAWFPSRAIAYQYWRDG
                     MGMLELWHRSEATDAHRALRFEVGFEMEVGGHLVRGRIDRVDPGPDGGIVLLDYKTAR
                     YPASEEEAERSLQLAIYDLAALRD"
BASE COUNT          286 a          807 c          927 g          275 t
ORIGIN      
        1 ggtcgcgcag ggccgccagg tcgtagatgg cgagctggag ggagcgctcg gcctcctcct
       61 cggacgcggg gtagcgggcg gtcttgtagt cgagcagcac gatgccgccg tccgggcccg
      121 ggtcgacccg gtcgatgcgc ccccggacca ggtggccgcc gacctccatc tcgaacccga
      181 cctcgaaccg cagcgcccgg tgggcgtcgg tggcctcgct gcgatgccac agctccagca
      241 tgcccatgcc gtcgcgccag tactggtagg cgatcgcccg ggacgggaac caggcggggt
      301 tgaaccgctc gcggtaggcg gccttgaggt cctccggctg gttggtgatc tcacccttgg
      361 cggccagctc gaagatggtg tgcatgagcc gcccgaaggc catctggtag gaggagcggt
      421 cgtcgagccc ggccacgctg ccgaagaagt acttcaggtg gcagtcctcg taggtcccga
      481 tccgggagta ggaggtgcgc aggcgcccct cggcggcgat cggctcgggg tcgacggtgt
      541 agtcccggcg ccaccaccag gtggccgggt cgacgccggg gatggcggcc agggtggccg
      601 cggcggccag ccgttcggcc gccggccggt cccggtcgcc ggcaacccgg cggagggcac
      661 cggcggcctc cagcgatccg agcggggccg tctccggggc tggcgggtcg gcggggagct
      721 cgaggccgag ctcgcggagg aaccgggacg gctcgccacg gccgtcgacg cgggaggccg
      781 tgaagaccac ccggtcccgg gcgcggctgg ccgcgagcgt gaagcgccgc cgctcggcgt
      841 cgaggagctc gttcgcgcgg accactgggc cggggtcgcc gtcgagccgc caggcctcga
      901 acagcccctg cgggcgggcg gtcatgggca gcgccccctc caggcacccg gcgacgatca
      961 ccaggtcgaa ctcctggccc ttggcggcgt tgaccgacag cacggcgacg gtgtcgttcc
     1021 tggtcatccc cggaggcagc caggggtcgc tggcgaagtc ggcccgctcg accacatcga
     1081 ggtaggtgcg catgctggcc cctggccggc cgtcgacgaa caggccgagg gcccgggaga
     1141 aggcggtcag ggcgtcgagc tggcgttgca cgtcggcgtc ggtggggtcg acctcggccc
     1201 gcaggatcag ctcggagaag gcgggcgcgg tccgccacac ctcccagaag caggcgtcgg
     1261 cgtccagctc cgcgacccag cgctcggccc cttcgagcag gtcgcacagc gcctggaccc
     1321 tggccacgag ccctgggtcg agcaccaggg ccgacccggt ccgggccggt cccacttctc
     1381 ccccatcgcc gtcggccgtg gaggcctccg cagcctccgc ttcctcgggg cccacggcct
     1441 ccggacctgg ggtgtcccgg ccgccgcctt cgcggtcggc cgggtcgagg tccggctccg
     1501 cgggaggtgg ggggacggcg cgggcgcgga catggtcggc gagggagcgg tcggcgagga
     1561 gggcggcgcg gcggagggca cgcagctcgc cagggtcgag gccgccgagg ggggaggtga
     1621 ggagggtggg gagcagctcg tcggcgcggg ccgggtcgag ggccaggcgg aacaggtcga
     1681 ggacgtgccc gacgaccggc tcggccacca gctggcgctc gccggcgccc agccggtagg
     1741 gaacgccgaa ccgctcaagg gcccggcggg tgggcgcgct gagccgccgg gtcgacggca
     1801 gcagcacggc catccggccg taggggacgc cgtcccgttc gtgggcgacc ttgagcaggc
     1861 gggcgttcgc ctccgcctcc tcggccggac cggggtagcg ggcggcctcg acgatcgtgg
     1921 taccgccccc ggtgagggcg acgaccgggc cggggccggc tgcctggagg cgccgcacgg
     1981 cgtcgagtcc gggaccgccg agccgccggc tgcgctcgag caggaccacg ctggcgccca
     2041 ggtccttggc caatgccggc agcgcccccg gggtggcacc acggaaggtg tcggtggcgc
     2101 ccgccgggtc gccggccagg atcgccctcg tgccgccatc caccagcagc gtgagcagcc
     2161 ggcgctgggc ggggtcgagc tcctgcgcct cgtcgaccag gatcgtgccg acctgcgcga
     2221 ccgtggccgc gaggatgtcg ggatggtctt cgagcaggtc gccggcctga aggatgaggc
     2281 cggcctggtc gacgg
//
LOCUS       DASSWR010000265         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_151710,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000265 DASSWR010000000
VERSION     DASSWR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151710"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..363)
                     /locus_tag="VFO47_04460"
     CDS             complement(7..363)
                     /locus_tag="VFO47_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET8787980.1"
                     /translation="MDQQTVDRLRERLETERGEVRRQLDDLGARRDAEGIEDPELDEG
                     FADAGQAAAERANLLTLVRSLRDTLRDVEQALDRMDAGTYGICERCGQPIPDERLEAL
                     PAARLCMTCKRQRGSR"
     gene            complement(493..1446)
                     /gene="xerD"
                     /locus_tag="VFO47_04465"
     CDS             complement(493..1446)
                     /gene="xerD"
                     /locus_tag="VFO47_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET8787981.1"
                     /translation="MPTAPVGPAAAAAVRRYLDYLAVERGLASNTLSAYRRDLHRYAG
                     WLAAAGIDDPARAGEPDLVAFLGALRAGSTPVGTRYRASSVARSLAAVRGFHRFLVRE
                     RLAGADPSRDLGTPKVPASLPKALSVEQVEALLGAIAGHDQLALRDRALLETLYAAGL
                     RVSEATALDVDDLDLEDGTVRAFGKGAKERLVPVGRSARRALEGYLVRGRPLLAGPRS
                     GPALFLNAQGSRLTRQGCWKILRRHARHAGLEAQVSPHVLRHSFATHLLAGGADIRSV
                     QELLGHASLATTQVYTRVTQDRLREVYLRAHPRARHATPSE"
     gene            complement(1480..>2482)
                     /gene="ald"
                     /locus_tag="VFO47_04470"
     CDS             complement(1480..>2482)
                     /gene="ald"
                     /locus_tag="VFO47_04470"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET8787982.1"
                     /translation="TDLEFEKSGATVLETADEVWGEADLILKVKEPVPEEFHRLRKDQ
                     VLFTYLHLAASREVTEALLDSGTTAVAYETVELPDGRLPLLAPMSEVAGRMAPQVGAH
                     LLERESGGRGVLMGGVSGVAAAKVLVLGGGMAGQNAAWIAQGMEAEVTLLDKNVDKLR
                     YVDQIHKGRIVTLMSNRQTIEDLAINADLIIGSVLIPGAKAPRLITAEHVRAMKRGAV
                     LVDISIDQGGCFETSHVTTHSDPTYVVDGVVHYCVGNMPGAVPHTSTYALTNVTLPYA
                     AQIASAGLEEAVKDDPPLAKGINVYRGELTYEQVAEAHALDWRPLPEVVPGLADAAG"
BASE COUNT          298 a          857 c          977 g          350 t
ORIGIN      
        1 ccgctgtcac cgcgaccctc gttggcgttt gcaggtcatg cagaggcggg cggcggggag
       61 ggcctcgagg cgctcgtcgg ggatgggctg gccgcagcgt tcgcagatgc cgtaggtgcc
      121 ggcgtccatc cggtcgaggg cctgctcgac gtcgcgcagg gtgtcgcgca ggctgcggac
      181 cagggtgagg aggttggcgc gttcggcggc ggcctgaccg gcgtcggcga agccctcgtc
      241 gagctccggg tcctcgatgc cctcggcgtc gcgtcgggca ccgaggtcgt cgagctggcg
      301 gcgaacctcg ccgcgctcgg tctcgagccg ttcccgcagc cggtcaacgg tctgctggtc
      361 catcccggtc ctccgtcgcg cttctgcccg ccgcaaccgg gagacggcac gacggtcggc
      421 cttcgagagc aggagtttga ggctacagga tcgtcgggcc agtgccaggg gtgcttgtcg
      481 gagatgtccg gttcattcgc tgggggtcgc gtggcgggct cgagggtggg cgcgcaggta
      541 gacctcgcgc aggcggtcct gggtgacccg cgtgtagacc tgggtggtgg ccaggctggc
      601 gtggccgagc agctcctgga cgctgcggat gtcggccccg ccggccagca ggtgggtggc
      661 gaacgagtga cgcagcacgt gcggggacac ctgcgcctcc agtccggcgt gccgggcgtg
      721 gcggcgcagg atcttccagc agccctggcg ggtcaggcgg ctgccctggg cgttcaggaa
      781 cagggcgggc cccgagcgcg gcccggccag cagcgggcgg ccccggacca ggtagccctc
      841 aagggcccgg cgggccgagc ggccgaccgg gaccaggcgc tccttggccc ccttgccgaa
      901 ggcccgcacc gtcccgtcct ccaggtccag gtcgtcgacg tcgagcgcgg tggcctcgct
      961 gacccgcagc ccggccgcgt acagggtctc gagcagggcc cggtcgcgca gggccagctg
     1021 gtcgtggccg gcgatcgcgc ccagcagcgc ctcgacctgc tccaccgaca gggccttggg
     1081 caggctggcc gggaccttgg gggtgccgag gtcgcgggag gggtcggcgc cggccagccg
     1141 ctcgcggacc aggaagcggt ggaacccgcg caccgcggcc aggctgcggg cgacgctgga
     1201 ggcccggtag cgggtcccga ccggggtgct gccggcccgc agcgcgccca ggaaggcgac
     1261 caggtcgggc tcgccggccc gggccgggtc gtcgatcccg gcggcggcca gccagccggc
     1321 gtagcggtgc aggtcccggc ggtaggcgct gagggtgttg gaggccaggc cccgctccac
     1381 ggccaggtag tcgaggtagc ggcgcaccgc cgccgcggcc gccggcccca ccggcgcggt
     1441 gggcacaccg gctggcggcg cggaggtgga catcggcggt tagccggcgg cgtcggccag
     1501 gccggggacg acctcgggca gggggcgcca gtcgagggca tgggcctcgg cgacctgctc
     1561 gtaggtgagc tcgccgcggt agacgttgat gcccttggcc agcggcgggt cgtccttgac
     1621 ggcctcctcg agaccggccg aggcgatctg ggccgcgtag ggcagggtga cgttggtgag
     1681 cgcgtaggtc gaggtgtggg gcaccgcccc gggcatgttg ccgacgcagt agtggaccac
     1741 cccgtcgacc acgtaggtcg ggtccgagtg ggtagtcacg tgcgaggtct cgaagcagcc
     1801 gccctggtcg atcgagatgt cgaccaggac cgcgccccgc ttcatcgccc ggacgtgctc
     1861 ggcggtgatc agccggggcg ccttggcccc cgggatcagg accgagccga tgatcaggtc
     1921 ggcgttgatg gccaggtcct cgatcgtctg ccggttcgac atcagggtga cgatgcggcc
     1981 cttgtggatc tggtcgacgt agcggagctt gtcgacgttc ttgtccagca gggtgacctc
     2041 ggcctccatg ccctgggcga tccaggcggc gttctggccg gccatgccgc cgcccaggac
     2101 cagcaccttg gccgcggcca cgcccgacac cccgcccatc agcaccccgc ggccgccgga
     2161 ctcgcgctcc agcaggtggg cgccgacctg gggggccatc cggcccgcga cctcgctcat
     2221 gggcgcgagc aggggcagcc ggccgtccgg cagctcgacc gtctcgtagg cgacggcggt
     2281 ggtcccgctg tccaggagcg cctcggtcac ctcgcggctg gccgccaggt gcaggtaggt
     2341 gaacagcacc tggtccttgc gcagccggtg gaactcctca gggaccggct ccttcacctt
     2401 gaggatgagg tcggcctcgc cccagacctc gtcggcggtc tccaggacgg tcgcccccga
     2461 cttctcgaac tccaggtcgg tg
//
LOCUS       DASSWR010000266         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_349401,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000266 DASSWR010000000
VERSION     DASSWR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349401"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2433
                     /gene="rpoC"
                     /locus_tag="VFO47_04475"
     CDS             <1..>2433
                     /gene="rpoC"
                     /locus_tag="VFO47_04475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET8787983.1"
                     /translation="NDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDN
                     GRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCG
                     LPKQMALELFKPFVMKRLVDLSYAQNIKSAKRMVERSRPQVWDVLEEVIREHPVLLNR
                     APTLHRLGIQAFEPVLVEGKAIQIHPLVCKAFNADFDGDQMAVHVPLSSEAQAEARVL
                     MLSVNNILSPANGRPIAIPTHEMVLGIYFLTYKEDAPPAQRNGQKKKRAEADASSPHR
                     PIFGSVAEAVMAHDFKSIGLQDPIVVRLPEAKAALVEDGVTKVETTVGRLLFNEALPE
                     DFPYINETLQSRELTSLVSRVADRYDQTETAEVLNKLKTLGFHWASRAGVTVSIQDVL
                     KPPRKQQILDEFEKRAEKVESQFRRGIITDDERRQELIEIWTEATERVREDMEERFRQ
                     EPFNPIYMMATSGARGNMTQVRQIAGMRGLVANPKGDIIPRPIKANFREGLSVLEYFI
                     STHGARKGLADTALRTADSGYLTRRLVDVTQDVIVRESDCGTERGIPVTVAVQRPIPG
                     KKGEFEWVRPRAVESQALSRILAEDVMDPAGKGKQVLLEAGTEIGEEVIDLLVEHGVD
                     EVLCRSVLTCDSKIGVCQLCYGRSLANNRLVDIGEAVGIIAAQSIGEPGTQLTMRTFH
                     TGGIAGEDITHGLPRVQELFEARTPKGMTPIAEFSGRVGIEHVEKGVQVTVTSDEGEE
                     WVAIVPRRARLRVEDGQDVEVGQPIIDGIPNPKEMLRVLGIRATQRHLVEQVQQVYTS
                     QGVTIHDKHIELI"
BASE COUNT          429 a          854 c          822 g          328 t
ORIGIN      
        1 aacgacctgt accggcgggt catcaaccgc aacaaccgcc tcaagcggct gctggacctg
       61 ggcgcgcccg agatcatcgt caacaacgag aagcgcatgc tccaggaggc cgtcgacgcg
      121 ctgttcgaca acggccgccg cggccgcccg gtcaccggtc cgggcaaccg gcccctcaag
      181 agcctgtccg acatgctcaa gggcaagcag ggccgcttcc gccagaacct gctcggcaag
      241 cgggtcgact actccggccg ctcggtcatc gtggtcggcc ccgagctcaa gctgcaccag
      301 tgcgggctgc ccaagcagat ggccctggag ctgttcaagc cgttcgtgat gaagcggctg
      361 gtcgacctgt cctacgcgca gaacatcaag tcggccaagc gcatggtcga gcgttcccgg
      421 ccccaggtct gggacgtgct cgaggaggtc atccgcgagc acccggtgct gctcaaccgg
      481 gccccgaccc tgcaccgcct gggcatccag gcgttcgagc cggtcctggt cgagggcaag
      541 gccatccaga tccacccgct ggtctgcaag gcgttcaacg ccgacttcga cggcgaccag
      601 atggccgtgc acgtgcccct gtcgtccgag gcccaggccg aggcgcgggt gctgatgctg
      661 tcggtcaaca acatcctgtc gccggccaac ggccgcccga tcgccatccc gacccacgag
      721 atggtgctgg gcatctactt cctgacctac aaggaggatg cgcctccggc gcagaggaac
      781 ggtcagaaga agaagcgcgc cgaggctgac gcctcgtcgc cgcaccggcc gatcttcggg
      841 tcggtggccg aggccgtcat ggcccacgac ttcaagtcga tcggcctcca ggacccgatc
      901 gtcgtgcggc tgcccgaggc caaggcggcc ctggtcgagg acggggtcac caaggtggag
      961 accacggtcg ggcgcctgct gttcaacgag gccctgcccg aggacttccc ctacatcaac
     1021 gagaccctgc agagccgcga gctgaccagc ctggtgtcgc gggtcgccga ccgctacgac
     1081 cagaccgaga ccgccgaggt cctgaacaag ctcaagaccc tcggcttcca ctgggcgtcg
     1141 cgggccggcg tcaccgtgtc tatccaggac gtgctcaagc cgccgcgcaa gcagcagatc
     1201 ctcgacgagt tcgagaagcg cgccgagaag gtcgagtccc agttccgccg gggcatcatc
     1261 accgacgacg agcgccgcca ggagctcatc gagatctgga ccgaggccac cgagcgggtc
     1321 cgcgaggaca tggaggagcg gttccgccag gagcccttca accccatcta catgatggcc
     1381 acctccgggg cccgcgggaa catgacccag gtgcgccaga tcgccggcat gcgcgggctg
     1441 gtcgccaacc ccaagggcga catcatcccg cggccgatca aggccaactt ccgcgagggc
     1501 ctgtcggtgc tcgagtactt catctcgacc cacggcgccc gcaaggggct ggccgacacg
     1561 gccctgcgca cggccgactc cggctacctg accaggcggc tggtggacgt gacccaggac
     1621 gtgatcgtcc gcgagagcga ctgcggcacc gagcggggca tccccgtgac cgtggccgtg
     1681 cagcggccca tcccgggcaa gaagggcgag ttcgagtggg tccggccgcg tgcggtcgag
     1741 agccaggccc tgtcccggat cctggccgag gacgtcatgg acccggccgg caagggcaag
     1801 caggtgctgc tcgaggccgg caccgagatc ggcgaagagg tgatcgacct gctggtcgag
     1861 cacggcgtcg acgaggtgct gtgccgctcg gtgctgacct gcgactccaa gatcggggtc
     1921 tgccagctct gctacggccg cagcctggcc aacaaccggc tggtcgacat cggcgaggcg
     1981 gtcggcatca tcgccgccca gtcgatcggc gagccgggca cccagctgac catgcggacc
     2041 ttccacaccg gcggcatcgc cggcgaggac atcacccacg gcctgccgcg ggtgcaggag
     2101 ctgttcgagg cccgcactcc caagggcatg acgccgatcg ccgagttctc cggccgggtc
     2161 gggatcgagc acgtcgagaa gggcgtgcag gtcaccgtga cctcggacga gggcgaggag
     2221 tgggtggcga tcgtgccccg gcgggcccgg ctccgggtcg aggacggcca ggacgtcgag
     2281 gtcggccagc cgatcatcga cggcatcccc aaccccaagg agatgctgcg ggtgctcggc
     2341 atccgggcca cccagcggca cctggtcgag caggtccagc aggtctacac cagccagggt
     2401 gtgaccatcc acgacaagca catcgagctg atc
//
LOCUS       DASSWR010000267         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_715528,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000267 DASSWR010000000
VERSION     DASSWR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715528"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..348
                     /locus_tag="VFO47_04480"
     CDS             55..348
                     /locus_tag="VFO47_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787984.1"
                     /translation="MRRPPPGYRRGARPIYPGVFKMLIYGFILWALALLGIELPFELP
                     RLVFVVLQVLVIWLLWKGVKAARGAPRNQVRLFSAQEHWVNAFTLQALILFLW"
     gene            complement(314..1459)
                     /locus_tag="VFO47_04485"
     CDS             complement(314..1459)
                     /locus_tag="VFO47_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018254198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HET8787985.1"
                     /translation="MVVGAGPAGAAAALEARRLRPDATVALVDRAAFPRDKACGDGIG
                     PHATDELAALGASGVLEGYPPIRGLRLRSPSGLEVAGDPARPNHVVPRAVLDARLVEA
                     ATAAGAELRRAGVRRLEQHDGMVVVDGELAARVVVGADGANSTVRRLLGAPPNPDRAL
                     AIAVRGYAPAPAGRPEQLIAWVAEGWPAYVWSFPTGTGVANVGYGLLRSRFHGDRAEL
                     HRRLHDLLPEADPDPASLRAHHLPFSSSRPPLGRGRVLLAGDAASLVNPLSGEGIYYA
                     LLSGRLAARAALTGPRDPVAAYRRLLTRALGRHLRHAAVLAKAIHAPALAEAGLGAAA
                     GSQRLFDTLVELGLGQARITPRLLAALPRGLVRARAATRGTGSAPEA"
     gene            complement(1485..1694)
                     /locus_tag="VFO47_04490"
     CDS             complement(1485..1694)
                     /locus_tag="VFO47_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001000 - bacterial-type RNA polymerase
                     core enzyme binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HET8787986.1"
                     /translation="MDDGRHPLPAEFERLQAPSSAYAPRQVVTYLCTREHVMSVPFAA
                     DAEVPEVWDCRCGQPATRVAAADAS"
     gene            complement(1760..2176)
                     /gene="mscL"
                     /locus_tag="VFO47_04495"
     CDS             complement(1760..2176)
                     /gene="mscL"
                     /locus_tag="VFO47_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET8787987.1"
                     /translation="MRSWGTEFKQFLLRGNVVDLAVGIVIGAAFAAVVQAAVTDLLTP
                     LVAAIFGQPDFNDLTFTINGSVFRYGHFLNVLIAFITIAFVVFFFVVKPINRLMELSR
                     RRESPDPSTRKCPECVSEIPIDARRCAFCTSEVAPV"
BASE COUNT          304 a          827 c          916 g          303 t
ORIGIN      
        1 gcccagggcc cggagggggt gcccgcggct atccacaggc cctagactgg acatatgcgc
       61 cgaccgccgc ccgggtatcg ccggggtgct cgcccgatct atccgggcgt cttcaagatg
      121 ctcatctacg ggttcatcct ctgggcgctg gcgctgctcg ggatcgagct gccgttcgag
      181 ctgccccggc tggtctttgt cgtgcttcag gtgctggtga tctggctgct gtggaagggt
      241 gtcaaggcgg cgaggggtgc tcccaggaac caggtgcggc tgttcagcgc ccaggagcac
      301 tgggtgaacg ccttcacgct tcaggcgctg atcctgttcc tctggtagcg gcccgggccc
      361 tgaccagccc gcgggggagg gcggccagca gccggggggt gatcctggcc tggccgaggc
      421 cgagctcgac cagcgtgtcg aacagccgct gggagccggc ggcagcgccg aggccggcct
      481 cggccagggc cggggcgtgg atcgccttgg ccagcacggc ggcgtggcgc aggtggcggc
      541 cgagggccct ggtgagcagg cgccggtagg cggcgacggg gtcccggggc ccggtcaggg
      601 cggcccgggc ggccagccgg cccgagagca gggcgtagta gatgccctcg cccgacaggg
      661 ggttgaccag cgacgcggcg tcgcccgcga gcaggacccg gccccggccg aggggcggcc
      721 gggacgagga gaacggcagg tggtgggcgc ggaggctggc cgggtcgggg tcggcctcgg
      781 ggagcaggtc gtggaggcgg cggtgcagct cggcccggtc gccatggaag cggctgcgca
      841 gcaggccgta gccgacgttg gccaccccgg tgccggtggg gaacgaccag acgtacgccg
      901 gccagccctc ggccacccag gcgatcagct gctcgggccg gcccgccggg gccggcgcgt
      961 acccgcggac ggcgatggcc agggcccggt ccgggttggg cggggcgccc agcagccgcc
     1021 ggacggtcga gttggccccg tcggcgccga ccaccacccg ggcggccagc tcgccgtcga
     1081 ccaccaccat cccgtcgtgc tgctccaggc ggcgcacccc ggcccggcgc agctcggccc
     1141 cggcggccgt ggccgcctcg accagccggg cgtcgagcac ggccctcggg accacgtggt
     1201 tgggccgggc cgggtcgccg gccacctcca gcccgctggg ggagcggagg cgcagccccc
     1261 ggatcggcgg gtagccctcc agcaccccgg aggccccgag ggcggccagc tcgtcggtgg
     1321 cgtgcgggcc gatcccgtcg ccgcaggcct tgtccctggg gaaggccgcc ctgtcgacca
     1381 gggccacggt ggcgtcggga cgcaggcgcc tggcttccag ggccgcggcc gcgccggccg
     1441 gcccggcgcc gaccaccacc aggtcgtaga gggtcctgct caggtcagga ggcgtcggcc
     1501 gccgccaccc tcgtggcggg ctggccgcag cggcagtccc agacctcggg cacctcggcg
     1561 tcggccgcga acggcaccga catcacgtgc tcccgggtgc acaggtaggt gacgacctgc
     1621 cggggcgcat aggcgctcga gggcgcctgc agccgctcga actccgccgg aagcgggtgc
     1681 cggccgtcgt ccatcgccac tccttcctcg ctctgctgcg cctagtattg gcgccgctgt
     1741 caatccgggc aagcccgtcc tagacggggg ccacctccga ggtgcagaag gcgcagcggc
     1801 gagcatcaat tgggatctcg ctcacgcact cggggcactt gcgggtgctc gggtcggggg
     1861 actcgcggcg ccgggacagc tccatcagcc ggttgatggg cttgaccacg aagaagaaca
     1921 ccacgaaggc gatcgtgatg aaggcgatca ggacgttcag gaagtggccg tagcggaaca
     1981 ccgacccgtt gatcgtgaag gtcaggtcgt tgaagtcggg ctggccgaag atcgccgcca
     2041 ccaggggggt gagcaggtcg gtgacggcgg cctggacgac cgcggcgaac gcggccccga
     2101 tgacgatccc gacggccagg tcgacgacgt tgcctcgaag caggaactgc ttgaactcgg
     2161 tgccccagga gcgcatgcgg gcgaccctcc ttccacgcgg cggtggttcc tccctcggcc
     2221 gcgcatgcta cggcaacggg taggatcggc ggcatgcgtc accgtttcgc ccttcttgcc
     2281 ctgctggtcg cgctggtcct ggcggtcgcc ggctgcggcg gcgacccgcc gcccgaccca
     2341 ccggcagcgg
//
LOCUS       DASSWR010000268         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_67106,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000268 DASSWR010000000
VERSION     DASSWR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67106"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFO47_04500"
     CDS             <1..753
                     /locus_tag="VFO47_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8787988.1"
                     /translation="AVLAPYLDGHGRPTGDHGIGMLAADELAAVVTALDGEGFDVHVH
                     AIGDRAVRDTLDAFQAAAAANGRRDARHQIAHLQFIHPDDRPRFRRLGVIANAQPFWS
                     CLDGYMRHLTLPFLDPERAGWQYPWASLRRAGAVLAFGSDWTVSTANPLEEIEVAVRR
                     VAPDDRDGEPFLPAERVDLPAALDAFTSGAAYALRLEAETGSVTPGKLADLAVLDRDP
                     FDEAAGPVGDARVLATLVEGEPVHADPSLDLG"
     gene            complement(760..1773)
                     /locus_tag="VFO47_04505"
     CDS             complement(760..1773)
                     /locus_tag="VFO47_04505"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET8787989.1"
                     /translation="MPAIVAPARVRPLWHDAPVIEATRFGRTLPQGGCVGVPAPASPF
                     ENRSDLLRGVEWWQARGYRVKLAGGIEARDAYVAGDAKARARDLEAMFADPEVDVVQC
                     LTGGFGSMQLVPHLDFELIADHPKAFCGYSDITALHVALRQRAGLATLYGPHLMTMGW
                     TEASAFTRDRLLAVLRGGGAGPVPPDPDDPFLRPIRGGRATGPLAGGDLWLLLQTMGT
                     PWEVELEGAILFFEEVDAPPWYIDGMLTQLGQAGKLAGVAGVVVGDLERCEWSEQRPE
                     WPRTKSLEQVLEEHLEPLGVPVLYKLPVGHAKHLATLPLGVVAPLDGDARTLTIDEPA
                     LRP"
     gene            1855..2871
                     /locus_tag="VFO47_04510"
     CDS             1855..2871
                     /locus_tag="VFO47_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787990.1"
                     /translation="MGGRAARRHHRAGRAGRRWLIAATGLVLAGSATAAAAGFGLDRG
                     PGAGPGSGGDPAPAAAGPAAVPDTSLPVATTTTLPPPPELPRGGRTLFPRYRVVGFYG
                     MQNLDVLGAGPPDVVAQRLLKVARPYATPGHPVMPMFELIATVAHPFPTPSGLYRTHQ
                     EDAVVRSFLKAVRRIDGILVLDVQPGRDDFVSSLRHWEPYLRQPDVGIALDPEFAMGP
                     GQVPGKQLGRTDAAAINRASAYVAGIVRRHRLPQKLFMVHQFRDSMIRDKARIAIRPG
                     LAMTWNADGFGVRSAKLEDYRSYTRDRRFHPGLKLFYENDVNLMRPREVLKLKPVPRV
                     INYQ"
BASE COUNT          370 a         1149 c         1040 g          362 t
ORIGIN      
        1 gccgtgctgg cgccctatct ggacggccac ggccgcccca ccggcgacca cgggatcggc
       61 atgctggccg ccgacgagct ggctgcggtg gtcaccgccc tggacggcga gggcttcgac
      121 gtccacgtcc acgccatcgg cgaccgggcc gtccgcgaca cccttgacgc cttccaggcg
      181 gcggccgcgg ccaacggccg ccgcgacgcc cgccaccaga tcgcccacct ccagttcatc
      241 caccccgacg accgcccccg cttccgccgc ctcggcgtca tcgccaacgc ccagccgttc
      301 tggtcgtgtc tcgacggcta catgcgccac ctgaccctgc cgttcctgga cccggagcgg
      361 gccggctggc agtacccgtg ggcgagcctg cgccgggccg gggcggtgct ggccttcggc
      421 agcgactgga cggtgtcgac cgccaaccct ctggaggaga tcgaggtcgc ggtgcgccgg
      481 gtcgcccccg acgaccgcga cggcgagccg ttcctgcccg ccgagcgggt cgacctgccc
      541 gccgccctcg acgccttcac cagcggcgcc gcctacgccc tgcgcctgga ggccgagacg
      601 ggctcggtca cccccggcaa gctggccgac ctggccgtcc tggaccgcga cccgttcgac
      661 gaggccgccg gccccgtcgg cgacgcccgc gtcctggcca ccctggtcga gggggagccg
      721 gtccacgccg acccctccct cgacctgggc tgagagcgct caggggcgca gggccggctc
      781 gtcgatggtg agtgttcgtg cgtccccgtc gaggggggcc accaccccca ggggaagggt
      841 ggccaggtgc ttggcgtggc cgacggggag cttgtacagc accgggaccc cgagcggctc
      901 caggtgctcc tccaggacct gctccagcga cttggtccgg ggccactccg ggcgctgctc
      961 gctccactcg caccgctcca ggtcgccgac caccaccccg gcgacgccgg ccagcttgcc
     1021 ggcctgcccg agctgggtga gcatcccgtc gatgtaccag ggcggcgcgt cgacctcctc
     1081 gaagaacagg atggccccct ccagctcgac ctcccagggc gtgcccatgg tctggagcag
     1141 cagccacagg tcgccgccgg ccagcggccc ggtggcccgg ccgccccgga tcgggcggag
     1201 gaaggggtcg tcggggtcgg gcggcaccgg gccggccccg ccgccgcgca gcaccgccag
     1261 cagccggtcg cgggtgaagg cgctggcctc ggtccagccc atcgtcatca ggtgcgggcc
     1321 gtagagggtg gccagcccgg cccgctggcg cagggcgacg tgcagggcgg tgatgtcgga
     1381 gtagccgcag aacgccttgg ggtggtcggc gatcagctcg aagtcgaggt gggggaccag
     1441 ctgcatcgac ccgaacccgc cggtcaggca ctggacgacg tcgacctcgg ggtcggcgaa
     1501 catcgcctcc aggtcgcggg cccgggcctt ggcgtcgccg gcgacatacg cgtcgcgggc
     1561 ctcgatgccg ccggccagct tcacccggta gccgcgggcc tgccaccact cgaccccgcg
     1621 cagcaggtcg gaccggttct cgaacgggct ggccggcgcg ggcaccccga cgcagccacc
     1681 ctggggcagc gtccggccga acctggtcgc ctcgatcacc ggggcatcgt gccacagcgg
     1741 ccggacccgg gccggcgcga cgatggccgg caagtcccga attggcccgc gtgagcggtt
     1801 ccccgatagg gtccgcagct ggaccgggag catcggagga cggagcgggt ccggatgggc
     1861 gggagagcgg cgcggcggca ccaccgggcc ggcagggccg ggcggcgctg gctgatcgcc
     1921 gccaccggcc tggtgctggc cgggtcggcg accgcggccg ccgccgggtt cgggctcgac
     1981 cgcggtcccg gcgccggccc ggggtccggc ggcgacccgg caccggcggc ggccggcccg
     2041 gcggccgtgc ccgacacctc gctgccggtg gccacgacca ccaccctccc gcccccgccg
     2101 gagctgcccc gcggcggccg gacgctgttc ccccgctacc gggtggtcgg gttctacggc
     2161 atgcagaacc tggacgtgct cggggccggc ccgcccgacg tggtcgccca gcgcctgctc
     2221 aaggtggccc gcccgtacgc cacccccggc catccggtga tgccgatgtt cgagctgatc
     2281 gccaccgtgg cccacccgtt cccgaccccc agcggcctgt accggaccca ccaggaggac
     2341 gcggtcgtcc gctccttcct caaggcggtc cgccgcatcg acgggatcct ggtgctggac
     2401 gtgcagccgg gccgggacga cttcgtcagc tcgctgcgcc actgggagcc gtacctgcgt
     2461 cagccggacg tcgggatcgc cctcgacccg gagttcgcca tgggccccgg ccaggtgccg
     2521 ggcaagcagc tgggccgcac cgacgccgcc gccatcaacc gggcctcggc gtacgtggcc
     2581 gggatcgtcc gccgccaccg gctgccccag aagctgttca tggtccacca gttccgcgac
     2641 agcatgatcc gcgacaaggc caggatcgcc atccggcccg gcctggccat gacctggaac
     2701 gccgacggct tcggggtccg ctcggccaag ctcgaggact accgctccta cacccgcgac
     2761 cgccgcttcc accccggcct caagctgttc tacgagaacg acgtcaacct gatgcgcccc
     2821 cgcgaggtcc tgaagctgaa gccggtcccg agggtgatca actaccagtg accggcgcgc
     2881 gccgctccgg gagcggcttg gtcagcagtt cgtccacgta g
//
LOCUS       DASSWR010000269         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_518829,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000269 DASSWR010000000
VERSION     DASSWR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518829"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFO47_04515"
     CDS             <1..600
                     /locus_tag="VFO47_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8787991.1"
                     /translation="MQGRVEEARQSLARGLSAFDELGVTLTSSVTHPASFVAMLAGDT
                     ATAEAHLRRDYRRLEDMGERNMLGTTAAFLAQALAAQGRTGEAERFIEVSRAAAEGED
                     LSAQMVWQGLRARILAERGELGEAEELARAAVALAARTDFLNQHGDALLELAAVLARA
                     GRAPEARVAVGEALGLYERKGNLVGVSRAGRWMERRARP"
     gene            639..956
                     /locus_tag="VFO47_04520"
     CDS             639..956
                     /locus_tag="VFO47_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787992.1"
                     /translation="MANSQFNSPATLVADGTVAVDGNLNVDPAGLLSPEVEFRFLLVQ
                     GDVVVQGRGRGTAGFWSGKTLLDQPPLQEGDALAIGLLVVARQDPPGYETFSWADRIR
                     LAP"
     gene            complement(922..2584)
                     /locus_tag="VFO47_04525"
                     /pseudo
     CDS             complement(922..2584)
                     /locus_tag="VFO47_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549800.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          342 a          922 c         1046 g          274 t
ORIGIN      
        1 atgcagggcc gggtcgagga ggcgcgccag tcgctggcca ggggcctgtc cgccttcgac
       61 gagctgggcg tgaccctgac ctcatcggtc acccacccgg cgagcttcgt ggccatgctg
      121 gccggggaca cggccacggc cgaggcccac ctgcgccgcg actaccggcg tctcgaggac
      181 atgggcgagc gcaacatgct cggcaccacc gcggccttcc tcgcccaggc gctcgcggcc
      241 cagggccgga ccggcgaggc cgagcggttc atcgaggtga gccgggcggc ggccgagggc
      301 gaggacctgt cggcccagat ggtctggcag gggctccggg ccaggatcct ggctgagcgg
      361 ggggagctcg gggaggccga ggagctggcc cgcgcggcgg tcgcgctggc ggcccggacc
      421 gacttcctca accagcacgg cgacgccctg ctcgagctgg ccgccgtcct cgcccgggcc
      481 gggcgggcgc ccgaggcccg ggtcgcggtc ggcgaggcgc tcggcctcta cgagcgcaag
      541 ggcaacctgg tcggcgtgtc ccgcgcgggc cgatggatgg aacggcgcgc ccggccgtga
      601 cagactcctt gcggcaaagc ggatcatggg gggcgaacat ggcgaactcg cagttcaaca
      661 gcccggcgac cctggtggcg gacggcaccg tcgcggtgga cggcaacctc aacgtcgatc
      721 ccgcgggcct gttgtcgccg gaggtcgagt tccggttcct gctggtgcag ggcgacgtcg
      781 tcgtccaggg caggggacgg ggaacggccg gcttctggag cgggaagacg ctcctggacc
      841 agccgccgct ccaggaggga gacgcgctgg ccatcggcct gctggtcgtc gccaggcagg
      901 atccgcccgg ctacgagacc ttcagctggg ccgaccggat ccgcctggcg ccgtagcctg
      961 agccaggccg cgcggcggcg ggtggtcgcg gggctcgttg cgccagcggc gccacagggt
     1021 ggcgtagggg ccgccggcgg cgaccagctc ggcgtgggtg cccgagctcg gtcacgcggc
     1081 cgtcctcgac caccgcgacc cggtcggcgt cgtgggccgt gtggaggcgg tgggcgatgg
     1141 cgaccacggt gcggccggcc aggaccgccg acagggagcg ctccaggtgg cgggcggcgc
     1201 gggggtcgag cagcgaggtg gcctcgtcga gcaccagggt gtgcgggtcg gccaggacca
     1261 ggcgggccag ggcgacctgc tgggcctggg ccgccgacag ggcgtgggcg ccggccccga
     1321 ccaccgtgtc gaggccctcg gggagggcgt cggcccagcc gagggcgtcg accgcgtcga
     1381 gggccgcgcg cagctcggcg tcgccggccc cggggcgggc cagggacagg ttgtcgcgca
     1441 gggtgccggc gaacacgtgc tgctcctgag tgacgagcac gacgtggccg cggagctcgt
     1501 caagggacag ctcgaccagg ccgacgccgc cgacggtcac cgacccggac cggggcgcgt
     1561 tgacgccggc gaggaggcgg ccgagggtcg acttgccggc gccgctgggg ccgaccacgg
     1621 ccagccgctc ccccgggcgc aggtccaggt cgaccccgtg gaggacgtcg cggccggggg
     1681 tgtaggcgaa gcggacgtcg cgggcgacca gccgctcgcc gtccgggcgg cggccggtgg
     1741 cctcccggtc ggccgggacg tgggcgaccc cgaggaggcg ggcgaaggcg gccccgccga
     1801 tctggaggtg gtcgagccag gacaggatca cgtcgagggg gtcgaccagc tggcgcatgt
     1861 agagggcgac ggcggtgacc tggccgacgc tggccagccc gcgggcgtgg agcaggccgc
     1921 cggccagcac ggcggtcgcc accgggagca gataggcgcc ctcgatcgcc gggaaccaca
     1981 ccgtccgcag gaacagggtg tagcgctcgg cccggtactc ctcgcggatg tcgtcgtcgg
     2041 tgcggcggat gcggcgctcc tggagccgca gcgcctccat ggtccggccg ccctcgaccg
     2101 tctcggcgac gccgccggtc agccgcgacc aggcggccat ctcgcgcagg tagccggccg
     2161 gggcgcggcg caggtaccag cgggtcgaca gccacagcag cggcaccacg accaggaccg
     2221 acgccatggt gatccagggc gagacggcca ccgccgcgcc gaccgtgagc gccgccgtca
     2281 ccgacgcgat cagggtctcg ggcacggcga agcgcaccac ctcggcgacg tcctcgacgt
     2341 cgcgggtgga gcgggccagc aggtcgccgg tgccggcctc ctcgaccgtg cccaggggca
     2401 gggcgagcac gccgtcaagg aactcctcgc gcagctcggc cagcacccgc tcgccgagga
     2461 cgtaggaggc cagccgggcc acccgcacga gcacgacctg ggcgaccagg gcggccagca
     2521 cggccacggc gatcccgtcc acccgtccgg cggtggtccc gccggtgacc tcgtcgacca
     2581 gcac
//
LOCUS       DASSWR010000270         7619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_321181,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000270 DASSWR010000000
VERSION     DASSWR010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321181"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="VFO47_04530"
     CDS             complement(<1..2278)
                     /locus_tag="VFO47_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8787993.1"
                     /translation="MGLDGARFSLYEAVAGFLREAAAVQPLMVVLEDLQAADASSLLL
                     LEFLARDPHGGRLLLVATYRDVTADRVHGAGDTMSQLVREGHLLSLRGLDRGEVRGLI
                     EALSGVEPSEAMAAAVHEATEGNPLFVRETVRLLGGDVTLQDPGRLRVPLSGSVRTAI
                     GRRLAPLSADAVQVLSVAAVVGREFDLSLVGPACELPIERILDGLSEAVALDVVIEGE
                     GAAGRYRFSHSLIREVLYERLPIPTRVDLHRRVGESIEHQYGGGLASHVAELARHFAE
                     AAAAGEAAKALAYARQAGERAMDLHAYEEAAVQYRRALQTLRFVGPDEPVRCDLLLRL
                     GAALARAGRYREAEESCLEAAELSRRLGSSEQLAQAALVFGARELGGGTVNRRLVGLL
                     REALDAVPASDSPVRARLLARLSLELTFSDETQLAGPISREAVELGRRLGDAAALAGA
                     LRARWMAVWGPDGLEERSAVAEELLRLARATGDQDLELAGRSRRATSALQSGDIGVVE
                     HDVVACARLAEELHLPSHRWMASTMRAMLALLQGSLGEAEALAEQARSSQPDGPNTAF
                     AYNDLIEALRWAQGRLQELRPAWQEQMVSTPWSAWPRLWLALTDTDHGDEAAARGSLQ
                     HLASGLLGRPRDGLWPPVMAVAALVAARLREPVAAGQLYEALFPYRNQVIVIAMPHPV
                     VCLGSASFYLGLLATVTSSWPEAGDHFEAAIRAHTQLGARPLLTRTRYEYARMLLARG
                     QATDGRRVAELLDQALATA"
     gene            complement(2738..3694)
                     /locus_tag="VFO47_04535"
     CDS             complement(2738..3694)
                     /locus_tag="VFO47_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8787994.1"
                     /translation="MTPDQDVAYGAHLPLIDLGTPRSQHGLGAYARAAAGLGYRYLCA
                     NDHLVFGRPWLDGPTALGASIESSGDMTLVTTASLPVLRGPVQLAKTLAAIDILSGGR
                     LVAGVGPGSSPGDYAAAGIGFQERWRRFDEALRVLRVLLHDDVHGFEGEFYSTRDVVL
                     QPRPVQRPGPPIWVASWGSPAGLRRVARLGDGWLASAYNTTPERFRESLDRLAEERRR
                     LGRTPGLLASAIATTWLHITEDKGEAERTLTGVLAPMLHRPAEALRSAPLLIGPAELC
                     AERLTAWIEAGARRIFVWPLADEARQLELFRERVTSLVPRQP"
     gene            complement(3706..4464)
                     /locus_tag="VFO47_04540"
     CDS             complement(3706..4464)
                     /locus_tag="VFO47_04540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8787995.1"
                     /translation="MGDAAAIGARTLLTSELLCESVDLRAGERVLDVPCGSGSGALAA
                     ARRFCRAVGVDVPQLLERAQRRAEAEGLEVAFLEGDAEQLPFPDGSFEVVLSAGDAIS
                     AHDQEGTVEELLRVCRPGGRIGMVCWTPDGYVGELFAAIGRQLPATAALEAPILGGTQ
                     ERLRELFGPEVAITAPRRSFLWRFPSAEHQVEYFGSFHDPTAKALQALGPDRAGALKA
                     ELLEVARRFDVSEDETLVLRLDYLEAVVRKPAWL"
     gene            4601..5575
                     /locus_tag="VFO47_04545"
     CDS             4601..5575
                     /locus_tag="VFO47_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8787996.1"
                     /translation="MLDPRRLRVLREVAGQGSFSAAAEALAFTQSAVSQQVAALERET
                     GTRLVERGVRPVRLTDAGRALLVHAEAVLARLDEAQQELGEIAGLRRGRLRLASFPTA
                     IATLVPRAVALFNQRHPDVDLTVVDEHQQGLVPMLARWELDLALIYDHEALPGPEVRL
                     ERTQLLDDPFELVVPDGHRLARRASVALEALAGETWIGGTPDGAYAQIILHSCRAAGF
                     EPRVVFGSDDYNAVQAFVAVGLGVAILPRLALAFPRPGLTRVALTDPPVRRIAAARLA
                     ASFRSAATASMLGVLKETAGTWAGVGRHDAPVTAGTAGRRLGRHASNG"
     gene            5888..6529
                     /locus_tag="VFO47_04550"
     CDS             5888..6529
                     /locus_tag="VFO47_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8787997.1"
                     /translation="MDVLVAGGHGKIALRLLGLLAAKGHRARGLIRKPEQAADLEAVG
                     AVAVLGDLEADASLAEYVEGADVVVFAAGAGPGSGPERKRTVDLGGAVKLVDAALAVG
                     VRRYVMVSSIGADRPQAASGGMRPYLAAKAEADDYLMASGLEYTIVRPGSLTDDPGTG
                     RVRVSTDLGSRGQIPRDDVAAVIAEVLDAPNTIGVTFELFSGDLLVVEAVRSL"
     gene            complement(6548..7546)
                     /locus_tag="VFO47_04555"
     CDS             complement(6548..7546)
                     /locus_tag="VFO47_04555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8787998.1"
                     /translation="MSRNRQPGETRPTHPGPRRTRRPMRPELRFRAGSLTKSLVAAVV
                     LRLAAEGRLSLADPVERWLPGILRYGDRVSLRQLLNHTGGVPHRTATLEQALYASRPG
                     RLRAWTPQALVAMVADQPPAFPAGTAWSYSNTGYVLLGLVVEAASPRALGQELGRAIL
                     GPLGLRDTVLADSAPELPGPSARGYSLPLGPGGEALDGPLLDFTVQNPSWAGAAGALV
                     STLPDLTRFFRALLGGRLLPPPLLAEMTTMVAVPPGSAPLPLYDRYGLGLVEVETPAG
                     PLLGNAGGIPGFLSIVLSTPDGRRQLGLMINALLAPDPVYEAFIQGSRALGGRLLS"
BASE COUNT         1036 a         2856 c         2796 g          931 t
ORIGIN      
        1 cggccgtggc cagggcctgg tccaggagct cggccacccg ccgcccatcc gtcgcctggc
       61 cccgagccag cagcatgcgg gcgtactcgt agcgggtccg ggtcagcaac ggcctggcgc
      121 cgagctgggt gtgggcgcgg atcgccgcct cgaagtggtc gccggcctcc ggccagctcg
      181 aggtcaccgt cgccaggagg ccgaggtaga acgacgccga ccccaggcac accaccgggt
      241 gaggcatcgc gatcacgatg acctggttcc ggtacgggaa cagcgcctcg tagagctggc
      301 cggcggcaac cggctcgcgg agacgtgcgg ccaccagggc ggcaacggcc atcaccggcg
      361 gccagaggcc gtcgcggggc cgcccgagga gcccgctcgc caggtgctgg agcgacccgc
      421 gggcggcggc ttcgtcgccg tggtcggtgt cggtcagggc cagccacagc ctgggccagg
      481 cagaccaggg cgtgctcacc atctgctcct gccaggcggg gcgcagctcc tggaggcgac
      541 cttgggccca gcggagcgcc tcgatcaggt cgttgtaggc gaaggcggtg ttcggtccgt
      601 cgggctgcga ggagcgggcc tgctcggcca gcgcctcggc ttcgcccagg gacccctgga
      661 gcagcgccag catggcccgc atcgtcgacg ccatccaccg atgggacggc aggtggagct
      721 cctcggccag ccgggcacag gccacgacgt cgtgctccac caccccgatg tcccccgact
      781 gcagcgcgct ggtcgcccgc cggctgcggc cggccagctc gaggtcctgg tcgccggtcg
      841 cccgggccag ccgcagcagc tcctcggcaa cggccgagcg ctcctccagc ccgtccggtc
      901 cccacacggc catccaccgt gcgcgcaggg cgcccgcgag ggcggcggcg tcgccgaggc
      961 gccggcccag ctcgaccgcc tcccggctga tcggcccggc cagctgcgtc tcgtccgaga
     1021 aggtcagctc cagcgacagg cgcgccagca gccgagcccg cacggggctg tcggacgccg
     1081 gcaccgcgtc cagggcctcc cgcaggagcc cgacgagccg ccggttgacc gtccctcccc
     1141 cgagctcgcg ggcgccgaac accagcgcgg cctgggcgag ctgctcgctc gagcccagcc
     1201 gccggctcag ctccgccgcc tccaggcagc tctcctccgc ctcccggtag cgtcccgccc
     1261 tggccagggc cgcgccgagc cgcagcagca ggtcgcagcg aaccggctcg tccgggccga
     1321 cgaaccgcag ggtctgcagc gcccgccggt actggacggc ggcctcctca tacgcgtgca
     1381 ggtccatggc gcgctcgccg gcctggcggg cgtaggccag cgccttggcc gcctcaccgg
     1441 cggccgcggc ctcggcgaag tggcgggcga gctcggcgac atgggatgcc aggccgccgc
     1501 cgtactggtg ctcgatcgac tcgccgacgc gccggtgcag gtccacccgg gtcgggatcg
     1561 gcagccgctc gtagagcacc tcccggatca gggagtgcga gaagcggtag cggccggcgg
     1621 cgccttcgcc ctcgatcacc acgtccagcg ccaccgcttc ggacagcccg tcgaggatgc
     1681 gctcgatcgg cagctcgcat gcgggcccga ccagcgacag gtcgaactcc cggcccacca
     1741 ccgccgccac cgacagcacc tgcacggcat cggcggacag cggggccagc cgccgtccga
     1801 tcgccgtccg cacgctcccg gacaacggca cccggagccg gccggggtcc tgcagcgtga
     1861 cgtcgccgcc cagcagccgc accgtctccc ggacgaacaa cgggttcccc tcggtcgcct
     1921 cgtggacggc ggcggccatc gcctcggagg gctccacccc cgagagggcc tcgatgaggc
     1981 cgcgcacctc gccccggtcc agaccccgga ggctgagcag atggccctcg cggaccagct
     2041 gcgacatcgt gtcgccggcc ccgtgcacgc ggtcggccgt cacgtcgcga taggtcgcca
     2101 ccagcagcag ccggcctccg tgggggtcgc gggccaggaa ctccagcaac agcagcgagg
     2161 aggcgtcggc cgcctgcagg tcctccagca ccaccatcag cggttgcacc gccgccgcct
     2221 cccgcaggaa cccggcgacg gcctcgtaca gcgagaagcg cgccccatcg aggcccacgc
     2281 gggcggtggc gggaccgtgg ccgccccgag gcgctcgccc aggccgggca ccagctgggc
     2341 gacctggccc gctcccgcgc ccagccaggc ggccatggtc tggtcgtcga ggccctccgc
     2401 gagcacctgg agcgtctgcg cccacggcca gaacgggggc gcgcctccgg cctcccagca
     2461 acggacccag tgcactcgag ctccccgcct ggccgcgtgg tccgccaggt gctgcgccag
     2521 ccacgtcttg ccgatgccag gctccccagc gatcagcaac acccgcatgc gtccggcgat
     2581 cgcgtcctcc aggccagcca ccaggtcggc cagctcccgg tcccggccca cgaacgcccg
     2641 ccctgccccc cgcccggcag gatgttccac gttcatcgcg ctgaccagcc taacccgagc
     2701 ccaggacgcc cgggacactc tccgccgggg tgcgcggcta cggttgccgt gggacgaggg
     2761 aggtgacgcg ctcgcggaac agctccagct gccgggcctc gtcggcgagc ggccagacga
     2821 agatccgccg cgcgcccgcc tcgatccagg cggtgagccg ctcggcgcac agctccgccg
     2881 ggccgatcag caggggggcg gaacgcagcg cctcggccgg ccggtgcagc atcggcgcga
     2941 gcacgccggt cagcgtccgc tcggcttcgc ccttgtcctc ggtgatgtgc agccaggtcg
     3001 tggcgatcgc gctcgcgagc aggcccggcg tcctgccgag gcgccgtcgt tcctcggcga
     3061 gccggtccag gctctcgcgg aaccgctcgg gcgtggtgtt gtaggcggac gcgagccagc
     3121 cgtcgcccag gcgggccacc cgtcgcagcc cggccggcga gccccagctc gcgacccaga
     3181 tcggcgggcc gggtcgctgc accggccgcg gttggagcac gacgtcgcgg gtcgagtaga
     3241 actcgccctc gaacccgtgg acgtcgtcgt ggagcagcac gcgcagcacc cgcagggcct
     3301 cgtcgaagcg tcgccatcgc tcctggaacc cgataccggc ggccgcgtag tcacccggcg
     3361 aggacccagg acccaccccg gcgaccaggc ggccgccgga caggatgtcg atcgcggcca
     3421 gagtcttggc cagctgcacc ggcccgcgca gcacgggaag gctggcggtg gtgacgagcg
     3481 tcatgtcccc tgacgactcg atcgacgcgc cgagcgcggt cgggccgtcg agccacggcc
     3541 ggccgaagac caggtggtcg ttggcgcaga ggtagcggta gccgagcccc gccgccgcac
     3601 gggcataggc cccgaggcca tgctgggacc gtggggtccc gaggtcgatc agcggcaggt
     3661 gcgctccata ggccacgtcc tgatccgggg tcatgcctcc ggacgctaca gccacgccgg
     3721 cttccggacg acggcctcca ggtagtcgag ccgcagcacc agggtctcgt cctcggacac
     3781 gtcgaagcgc ctggccacct ccagcagctc ggccttcagg gcgccggcac ggtccgggcc
     3841 cagcgcctgc agcgccttgg cggtcgggtc gtggaagctg ccgaagtact ccacctggtg
     3901 ctcggccgat gggaagcgcc acaggaagct gcgccgcggg gcggtgatgg ccacctcggg
     3961 accgaacagc tcgcgcagcc gctcctgggt gccccccagg atcggcgcct cgagggctgc
     4021 cgtcgccggg agctgcctgc cgatggcggc gaacagctcg ccgacatagc cgtcgggggt
     4081 ccagcagacc atgccgatcc gcccacctgg ccggcagaca cggagcagct cctcgaccgt
     4141 gccctcctgg tcgtgggccg agatggcatc accggccgac agcacgacct cgaaggagcc
     4201 gtcggggaac gggagctgct cggcgtcgcc ctccaggaag gccacctcca ggccctcggc
     4261 ctcggcgcgt cgctgggccc gctccagcag ctggggaacg tcgacgccca cggcccggca
     4321 gaagcggcgg gcggcggcca gtgcgccgct gccgctgccg cagggtacgt ccagcacgcg
     4381 ctcaccggcg cgcaggtcga ccgattcgca gagcagctcg ctggtgagca gggtccgcgc
     4441 tccgatggcg gcggcgtcgc ccaccgaccg gctggatccc aggagcttcc tttcagggct
     4501 ggcgcgcacg ggactcctct cgaagtgctg tgacctcaag gctcgccgga tcagtgaatc
     4561 aggtcaagtt acagattctt cactttgata agctctgctt atgctggatc ctcgccggct
     4621 ccgtgtgctg cgggaggtcg ccggccaggg ctcgttctcg gccgcggccg aggcgctcgc
     4681 gttcacgcag tcggcggtca gccagcaggt cgccgccctc gagcgggaga cgggcaccag
     4741 actggtcgaa cgtggcgtgc gaccggtccg cctcaccgat gccggtcgcg ccctgctggt
     4801 gcacgccgag gcggtcctcg cccgcctgga cgaggcccag caggagctcg gggagatcgc
     4861 cgggttgcgg cggggccgcc tgcggctggc cagcttcccc accgccatcg ccacgctcgt
     4921 cccccgggcc gtcgcgctgt tcaaccagcg ccaccccgac gtcgacctga ccgtggtcga
     4981 cgagcaccag caggggctgg tcccgatgct tgcccgctgg gagctggacc ttgccctcat
     5041 ctacgaccac gaggccctgc ccgggcccga ggtgcgtctg gagcggaccc agctgctgga
     5101 cgacccgttc gagctcgtcg tgcccgacgg ccaccgcctc gctcgccgcg cgtcggtcgc
     5161 tctggaggcg ctcgccggcg agacctggat cggcggcaca ccggacggcg cctacgccca
     5221 gatcatcctc cacagctgcc gggcggccgg cttcgagcca cgcgtcgtct tcggcagcga
     5281 cgactacaac gccgtgcagg cctttgtcgc tgtcggcctg ggggttgcca tccttccccg
     5341 cctggcgctc gcgttcccgc gaccgggcct cacgcgcgtc gccctgaccg acccgccggt
     5401 gcggcgaatc gccgccgccc ggctggcggc cagcttccgc tcggcggcca cggcgtcgat
     5461 gctcggggtc ctgaaggaaa cggcgggtac ctgggctggt gtcggacggc acgatgcgcc
     5521 ggtgacggcc ggcaccgccg gccgccgcct cgggcgtcat gcttcgaatg gataggcgac
     5581 aactggcgcc cggcatgtca aggaggaact gacatggcca ccaagcagaa gcaggcgctc
     5641 gacgaccgcg ggttgatccg ccaccagctg cggctcggag ctgggttgat cgcgtatggt
     5701 caccacagct cgtccgtggg cggccagtga catgccccgg accctgcgtc agctctgggc
     5761 cgacgaggac gacgctcacg accgggagtt cgcgtacaag cggcgagcga cccgtcggcg
     5821 cgccaagcag gccggccgtg cccgcaggcc ggtcgtgatc catgggaagg ctcagtagac
     5881 ttcacccatg gacgtcctgg tggccggtgg ccacggaaag atcgccttgc gcctgttggg
     5941 gctgctggcc gcgaagggtc atcgcgcccg tggcctgatc cgcaagcctg agcaggccgc
     6001 cgacctcgag gcggtcggcg cggtggcggt cctcggcgac ctggaggccg acgcgagcct
     6061 ggccgagtac gtcgagggcg ccgacgtggt ggtcttcgcc gccggcgcgg gcccgggcag
     6121 cggtccggaa cgcaagcgga ccgttgacct cggcggtgcc gtcaagctcg tcgacgcggc
     6181 cctggcggtc ggcgtgcgcc ggtacgtgat ggtcagctcg atcggggcgg accggccgca
     6241 ggccgcgtcc ggcggcatgc ggccctacct cgcggcgaag gccgaggccg acgactatct
     6301 gatggcgagc ggcctggagt acacgatcgt ccgccccggc tcgctcaccg acgatccggg
     6361 gaccgggcgg gtccgcgtgt ccacggacct gggcagtcgc ggacagatcc cgcgcgacga
     6421 cgtcgcggcg gtcatcgccg aggtgctcga cgccccgaac acgatcggcg tgaccttcga
     6481 actgttcagc ggcgacctcc tggtggttga ggccgtccgg tcgctctgac cgccgggacc
     6541 gggcacctca gctcaggagg cggccgccga gcgcccggga gccttggatg aacgcctcgt
     6601 agacggggtc cggggccagc agggcgttga tcatgaggcc gagctgccgg cggccgtccg
     6661 gggtgctgag gacgatgctc aggaagccgg ggatgccgcc ggcgttgccg agcaggggac
     6721 cggcgggcgt ctcgacctcc accagtccca ggccgtagcg gtcgtacagg gggagcgggg
     6781 cggaaccagg ggggaccgcg accatggtcg tcatctcggc caacagcggg ggcggcagca
     6841 gccggccgcc cagcagggcc cggaagaagc gggtcaggtc gggaagcgtg gacaccaggg
     6901 cgccggcagc cccggcccac gaggggttct ggacggtgaa gtccagcagg gggccgtcga
     6961 gcgcctcccc gcccgggccc agcgggaggc tgtagccacg cgcgctcggc cccgggagct
     7021 cgggggcgct gtccgcgagc acggtgtcgc gcaggccgag cggcccgagg atggcccggc
     7081 ccagctcctg cccgagggcc cgggggctgg ccgcctccac gaccaggccg agcaggacat
     7141 agccggtgtt ggagtacgac caggccgtac cggccgggaa ggccggcggc tggtcggcga
     7201 ccatggcgac cagggcctgg ggcgtccagg cacgcaggcg gcccggccgg gaggcgtaca
     7261 gcgcctgctc gagggtggcc gtgcgatggg gcacgccgcc ggtgtggttg agcagctggc
     7321 ggaggctgac ccggtcgccg tagcgcaaga tgccgggcag ccagcgctcc acggggtccg
     7381 ccagcgacag gcggccctcc gcggccagcc gcaggacgac ggcggcgacc agcgacttgg
     7441 tcaggctgcc ggcccggaag cgcagctccg gccgcatcgg ccggcgggtc cgccgggggc
     7501 cgggatgtgt cggacgggtt tcgcccggct ggcggttgcg cgacagtccg cccgagcctg
     7561 gtgcccggcg gtgccgagcg ggtgtaggtg atgtcggacc ggacccccga ccagacccg
//
LOCUS       DASSWR010000271         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_547706,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000271 DASSWR010000000
VERSION     DASSWR010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547706"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VFO47_04560"
     CDS             <1..971
                     /locus_tag="VFO47_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8787999.1"
                     /translation="VLAAAAGRGRPVTTGFPSTTSTSLASIGTGLPPGRHGLVGYTMV
                     LPGMERAFNCLRWSPYGLGGTKDLRDRFVPERVQPEPTAFEVAVADGIEVTSVGAGQF
                     AASGFTRAALRGGEYWRTHSLGDQADGALRGLGKGRRSLVYLYHPDLDRTGHVRGTDG
                     QAWRLELAHVDQLVGQIAERLPGDAALFVTGDHGMVDLRPSERVDLADRPELAAGVRL
                     LAGEARARHVHTERGAAGDVLAAWRGLLGQAMWVVSGEEAVAAGWFGPQVADQARARI
                     GDVVAAAHGPIGVVQREVDPTQAVLTGHHGSMTTAEQLVPFLELRR"
     gene            complement(1054..1227)
                     /locus_tag="VFO47_04565"
     CDS             complement(1054..1227)
                     /locus_tag="VFO47_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788000.1"
                     /translation="MDGRRDRDYDGVPDWQEEANYADEAPIRGNRARVRGSGCALPVA
                     LLAVAAGVVIRRW"
     gene            complement(1299..1892)
                     /locus_tag="VFO47_04570"
     CDS             complement(1299..1892)
                     /locus_tag="VFO47_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET8788001.1"
                     /translation="MSKVVTQASMSLDGFIADPSGGVGPLFEWYGNGDVEVTGADPER
                     VFRVSAASAAYLNEAWADAGVGVIGRRLFDITNGWNGRPPVGDAVVVVTHRPPADWDF
                     PDAPFTFVTDGVPAAVAQAKELAGDRYVSVNPGSIAGQAFAAGLVDEVRVDLAPVVLG
                     AGVRYFGDYAGSPLLLENPEIVQGDRVTHLHYRVRRA"
BASE COUNT          242 a          815 c          728 g          245 t
ORIGIN      
        1 ccgtcctggc cgccgcggcc gggcggggcc ggccggtcac caccgggttc ccgtcgacca
       61 cctcgaccag cctggcctcg atcggcacgg ggctgccccc gggccggcac ggcctggtcg
      121 gctacaccat ggtcctgccc ggcatggagc gggcgttcaa ctgcctgcgc tggtccccct
      181 acggcctcgg cgggaccaag gacctgcgcg accggttcgt gcccgagcgg gtccagcccg
      241 agccgaccgc cttcgaggtc gccgtcgccg acgggatcga ggtcacctcg gtcggggccg
      301 gccagttcgc cgcctccggc ttcacccggg ccgccctgcg cggcggcgag tactggcgca
      361 cccactccct cggcgaccag gccgacgggg ccctgcgcgg cctcggcaag ggccggcgca
      421 gcctggtcta cctctaccat cccgacctgg accgcaccgg ccacgtccgc gggaccgacg
      481 ggcaggcctg gcggctggag ctggcccacg tcgaccagct ggtcggccag atcgccgagc
      541 gcctccctgg cgacgccgcc ctgttcgtca ccggcgacca cggcatggtc gacctgcgcc
      601 cctccgagcg cgtcgacctg gccgaccggc cggagctggc cgccggggtg cggctgctgg
      661 ccggcgaggc ccgggccagg cacgtccaca ccgagcgggg cgccgccggc gacgtgctcg
      721 ccgcctggcg ggggctgctc ggccaggcca tgtgggtcgt gtccggcgag gaggccgtcg
      781 ccgccggctg gttcggtccc caggtggccg accaggccag ggcccggatc ggcgacgtgg
      841 tcgcggccgc ccacggcccc atcggcgtgg tccagcggga ggtcgacccg acccaggccg
      901 tcctcaccgg ccaccacggc tccatgacca cggccgagca gctggtcccg ttcctcgagc
      961 tgcgtcgctg accgcccagc gaactcccgg gaaggtcaag gcgccgcatg ctgggcctgt
     1021 ccggggacgc cgacagcggc taggtggccg cgctcaccat cggcgtatga cgaccccggc
     1081 ggccaccgcc aggagggcga ccgggagggc gcagccgctg ccgcgcacgc gggcccggtt
     1141 gccgcggatg ggggcctcgt cggcgtagtt cgcctcctcc tgccagtcgg gcacgccgtc
     1201 gtagtcccga tccctgcggc cgtccatggc ccctcccctc gtcggctcgc gacccgccag
     1261 cgcgccacga agcggtcgga gtatatgcct ccccgacgtc aggcccggcg cacccggtag
     1321 tgcaggtggg tcacccggtc cccctggacg atctccgggt tctccagcag caggggcgac
     1381 ccggcgtagt cgccgaagta gcggacgccg gcgccgagca cgaccggggc caggtccacc
     1441 cggacctcgt cgaccagccc ggccgcgaac gcctggccgg cgatgctgcc cgggttgacc
     1501 gagacgtagc ggtccccggc cagctccttg gcctgggcga cggcggcggg cacgccgtcg
     1561 gtgacgaagg tgaacggggc gtccgggaag tcccagtcgg ccggcggccg gtgggtcacc
     1621 accaccaccg cgtcgcccac cggcggccgg ccgttccagc cgttggtgat gtcgaacagc
     1681 cgccgcccga tcacccccac cccggcgtcc gcccacgcct cgttcaggta ggcggcgctc
     1741 gccgccgaca cccggaacac ccgctccggg tcggccccgg tgacctcgac gtcgccgttc
     1801 ccgtaccact cgaacagcgg cccgaccccg cccgacgggt cggcgatgaa cccgtccagc
     1861 gacatcgagg cctgcgtgac aaccttgctc atggggttcc ctccgatcgg tgggctcggt
     1921 cgagcctacg acgccggggg aacagccgcc ggcgccaggg ccgcgtcgac gctgaggcgc
     1981 cggccttcct gccaggccgc ctcggcggct accccgggaa gacgggcccg
//
LOCUS       DASSWR010000272         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_180199,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000272 DASSWR010000000
VERSION     DASSWR010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180199"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFO47_04575"
     CDS             complement(<1..465)
                     /locus_tag="VFO47_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788002.1"
                     /translation="MSDNFRPQGGCGVAQERRKVGSVSYQEAGDDYFGKRQLRRHAAF
                     WSLWSLGVAAVISGDFYGWNLGLDAGGFGGLLLATAVITIMYFGLCFSIAEMSPALPH
                     TGGAYSFARSAMGPWGGFITGLAENMEYVITPAVVVGAMGFLMHDIVAGLFNL"
     gene            558..1325
                     /locus_tag="VFO47_04580"
     CDS             558..1325
                     /locus_tag="VFO47_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8788003.1"
                     /translation="MQRPQRGTGARPRLRSVSVQDELKQRIDRGELPAGTRLPSEPEL
                     AAELQVSRATLREALRAMELEGLLRRRQGSGTFVASHPRMANSLDVNFGVTDAIRAAG
                     MTAGIASGRHWVEPASAGEAALLELEPGQDVLVIERVRTAEGKPVVLSRDVFPSRLLD
                     RHPGAAEDMMERSIYDVLERELGVVINHGVARFRPVRADHAVAGRLGVPRGELLLYLW
                     QVDYAMDGAPVLSSHEFHLADAFDFTVVRRGPGRRFT"
     gene            1322..2173
                     /locus_tag="VFO47_04585"
     CDS             1322..2173
                     /locus_tag="VFO47_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788004.1"
                     /translation="MTAQATTQVGTAMRLKRVIDPAGVSIICALDHGMTSPTFLEPLA
                     DIGGRTAEVVAGGANVIMMSKGMIRLAQPAFAPTTSLALLLSASANPGDPQPEIVQIA
                     EVGEAARLGADAVVLFTALGGASEAGMIRTLAGIGRECAAMGMPLIAEAEFPTTYAAV
                     EQLKEQYGFEYLRRNVRLCAELGADIVKTNWPGDGDSFARLVEAAAGIPLVLAGGSRL
                     GDRELLARMEAATAAGGIGCSVGRNIFMHRSPEAITRALSRVIRERWSADKAYAELQE
                     TAPEEPA"
     gene            2170..>3026
                     /locus_tag="VFO47_04590"
     CDS             2170..>3026
                     /locus_tag="VFO47_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET8788005.1"
                     /translation="MRAAFMTGRQQVEVRETGEPALDPRGAVLRVEACGICGTDARTF
                     ANGDPRAPAPWVLGHEPVGVLERVGPDAALPPGVEAGGRVFLGSILTCGECLQCIDGR
                     QNLCEQHLLYGYDPFPGAYAELAAVPPIAVKNLIALPPDLPSELATVVDPFACAFNGI
                     EVLDVDLGDTVLILGAGPIGCWQAVMARDRGASRVFLSDVNRQRLDLALSAVGGFVDD
                     AWVAGTDNGLAALLERTAGLGAERISVAAPSKQAQQAALEMAAKRARVVYFAGLPKHD
                     PVSPLDMNQL"
BASE COUNT          416 a         1125 c         1075 g          410 t
ORIGIN      
        1 gaggttgaac agcccggcga cgatgtcgtg catcaggaag cccatggccc ccaccaccac
       61 ggccggggtg atcacgtact ccatgttctc ggccagcccg gtgatgaagc cgccccacgg
      121 ccccatggcc gagcgggcaa aggagtaggc gccgccggtg tggggcaggg ccggcgacat
      181 ctcggcgatg ctgaagcaca gcccgaagta catgatcgtg atgaccgccg tcgcgagcag
      241 cagcccgccg aacccgccgg cgtcgagccc caggttccag ccgtagaagt cgcccgagat
      301 gacggccgcc acccccagcg accacagcga ccagaacgcg gcgtgccgcc gcagctggcg
      361 tttgccgaag tagtcgtccc cggcttcctg gtagctcacc gatccgactt tgcgtcgctc
      421 ctgagccacg ccacaacccc cttgcggtcg gaagttgtct gacattactt cacgctgccg
      481 ggatcgtaag tgggtggtcg aaccgagtca agctccttga ccctggctga gttgtctgac
      541 gtatactcct cgccagaatg cagaggccac agcggggaac cggggcgcgc ccacggctac
      601 gctcggtctc ggtccaggac gagctcaagc agcggatcga ccggggagag ctccccgccg
      661 gcacccgcct gccctccgag ccggagctgg ccgccgagct ccaggtgtcg cgggcgaccc
      721 tgcgcgaggc gctgcgggcc atggagctcg agggcctgct gcggcgccgc cagggctcgg
      781 gcaccttcgt cgcctcccat ccccggatgg ccaacagcct ggacgtgaac ttcggggtca
      841 ccgacgccat ccgtgccgcc ggcatgaccg ccgggatcgc ctccgggcgc cactgggtgg
      901 agccggcctc agccggcgag gcggccctgc tggagctgga gccgggccag gacgtgctgg
      961 tgatcgagcg ggtgcgcacc gccgagggca agccggtggt gctgtcgcgc gacgtgttcc
     1021 ccagccggct cctcgaccgg cacccggggg ccgccgagga catgatggaa cgttcgatct
     1081 acgacgtcct ggagcgggag ctgggcgtcg tcatcaacca cggcgtggcc cggttccggc
     1141 cggtgcgggc cgaccacgcc gtggccggcc gcctcggcgt gccccgcggc gagctcctcc
     1201 tctacctgtg gcaggtcgac tacgccatgg acggcgcccc ggtgctctca tcgcacgagt
     1261 tccacctcgc cgacgccttc gacttcacgg tggtccggcg gggccccgga aggaggttca
     1321 catgacagca caggccacca cccaggtggg caccgcgatg cgtctcaagc gggtgatcga
     1381 cccggccggg gtctcgatca tctgcgccct cgaccacggc atgacctcgc ccacgttcct
     1441 ggagccgctg gccgacatcg gcggccgcac ggccgaggtg gtggccggcg gcgccaacgt
     1501 catcatgatg tccaagggca tgatccgcct cgcccagccg gcgttcgcgc ccaccacctc
     1561 gctggccctg ctgctgtcgg cgtcggccaa cccgggcgac ccccagcccg agatcgtcca
     1621 gatcgccgag gtgggggagg ccgcccgcct cggggccgac gccgtggtgc tgttcacggc
     1681 gctgggcggc gcctccgagg cggggatgat ccggaccctg gccgggatcg gccgcgagtg
     1741 cgcggccatg ggcatgcccc tgatcgccga ggccgagttc cccaccacct acgccgccgt
     1801 cgagcagctc aaggagcagt acggcttcga gtacctgcgg cgcaacgtgc ggctctgcgc
     1861 cgagctgggc gccgacatcg tcaagacgaa ctggccgggc gacggcgact ccttcgcccg
     1921 gctggtcgag gcggccgccg gcatcccgct ggtcctggcc ggcgggtcgc gcctcggcga
     1981 ccgggagctg ctggctcgga tggaggcggc caccgcggcc ggcggcatcg gctgctcggt
     2041 gggccggaac atcttcatgc accgctcccc ggaggcgatc acccgggccc tgtcgcgggt
     2101 gatccgggag cgctggagcg ccgacaaggc ctacgccgag ctccaggaga ccgcgccgga
     2161 ggagccggca tgagggccgc gttcatgacc ggccggcagc aggtcgaggt ccgggagacc
     2221 ggcgagccgg ccctcgaccc gcgcggggcc gtcctccggg tcgaggcctg cgggatctgc
     2281 gggaccgacg cccgcacctt cgccaacggc gacccgcggg ccccggcccc ctgggtcctc
     2341 gggcacgagc cggtcggggt gctggagcgg gtcgggcccg acgccgccct ccccccgggg
     2401 gtggaggcgg gcgggcgggt gttcctcggc tccatcctga cctgcggcga gtgcctgcag
     2461 tgcatcgacg gccgccagaa cctgtgcgag cagcacctcc tctacggcta cgacccgttc
     2521 cccggggcct acgccgagct ggcggccgtg cccccgatcg cggtcaagaa cctcatcgcc
     2581 ctcccccccg acctgccgtc ggagctggcc accgtcgtcg acccgttcgc ctgcgcgttc
     2641 aacggcatcg aggtccttga cgtcgacctc ggcgacaccg tgctgatcct cggcgccggg
     2701 ccgatcggct gctggcaggc ggtgatggcc cgcgaccgcg gggccagccg ggtgttcctc
     2761 tcggacgtga accgccagcg cctcgacctg gccctgtccg cagtgggcgg gttcgtggac
     2821 gacgcctggg tcgccggcac cgacaacggc ctggccgccc tgctggagcg gaccgccggg
     2881 ctgggggccg agcggatcag cgtggccgcg ccctccaagc aggcccagca ggcggccctg
     2941 gagatggccg ccaagcgggc ccgggtcgtc tacttcgccg gcctgcccaa gcacgacccg
     3001 gtgagcccgc tcgacatgaa ccagct
//
LOCUS       DASSWR010000273         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_715747,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000273 DASSWR010000000
VERSION     DASSWR010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715747"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..>2450)
                     /locus_tag="VFO47_04595"
     CDS             complement(103..>2450)
                     /locus_tag="VFO47_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET8788006.1"
                     /translation="VLRNMLYEGSPTLGGLRYVVMDEVHYLQDRVRGAVWEEVLINLP
                     VEVRVASLSATVSNAEEFGEWLEATRGATEVVIEERRPVPLDNHYLIGRELHPMFVTR
                     GDDVVPNPALARRGEREWRPGRDTKGRPGRNGPRPVGRGWVPSRVEVVDLLEAERLLP
                     AIYFIFSRAGCDQALEQCRAGNIRLTSHAERAEILEFLELKVAPIDPADLDALGYDLL
                     AESLGRGVASHHAGMLPLFKEAVEELFAQGLVKVVFATETLSLGINMPARTVAIERLT
                     KWQGERHELLTPGEYTQLTGRAGRRGIDSRGAAVVLHQPYIPFERITGLASTRTYPLT
                     SSFRPSYNMAVNLVANYERGQAERLLASSFAQFLADRTVHGAEQTMARNARFLAGYLE
                     SAACDRGDITGYWELRRRLRARESTLADADRRAETQEAAEALRSLTPGTVIWLPGGRR
                     RGLAAVIGPAGGRDNSTGILVLTEDRRVRRVAVRDLGGIPVRVGKVAMPRQLSPKSPR
                     FKSYVARALAELDPPSVPRPRRRRRPGADDPELAALRDELRAHPVHGCPDLAEHEKWM
                     QRHDQLAKEQRALAERVRRRTGSLVRTFDRVLEVLGSLGYVDRFALTPKGETLRRVYA
                     ETDLVVVEAMHRGVWRGLEPAELAACVSSLVYETRGADGPPGDPPLAPTRAVEAALDA
                     LAKLQEEVRAHEEAGGLSLTRELDPGFADPAHRWASGDPLEDVLAAEDITPGDFVRVT
                     KQLIDLLRQIALVADDPEVQTTARAAVDACQRGVVAYSGLL"
BASE COUNT          310 a          874 c          952 g          314 t
ORIGIN      
        1 gggggctgtg tcgggaccac cgaccagggc ccgaacgacc tcgccgaccg agcaggtacc
       61 acctggagcc cgacggcccg gggccgggcc cgacccaccc ttctacagca agccagagta
      121 ggcgaccacg ccgcgctggc aggcgtcgac ggcggcgcgg gcggtggtct ggacctcggg
      181 gtcgtcggcg accagggcga tctggcggag caggtcgatg agctgcttgg tgacccggac
      241 gaagtcgccg ggggtgatgt cctcggcggc caggacgtcc tcgagggggt cgccgctggc
      301 ccagcgatgg gcggggtcgg cgaagccggg gtcgagctcg cgggtcaggg acaggccgcc
      361 cgcctcctcg tgggcgcgga cctcctcctg gagcttggcc agggcgtcaa gggcggcctc
      421 gacggcccgg gtgggcgcga gcggcgggtc gcccgggggg ccgtcggcgc cgcgggtctc
      481 gtagaccagg ctggagacgc aggcggccag ctcggccggc tcgagcccgc gccagacccc
      541 gcggtgcatg gcctccacca ccaccaggtc ggtctcggcg tacacccggc gcagggtctc
      601 gcccttgggg gtgagggcga agcggtccac gtacccgagg ctgccgagca cctccaggac
      661 ccggtcgaag gtccggacca gcgacccggt gcgccgccgc acccgctcgg ccagggcccg
      721 ctgctccttg gccagctggt cgtggcgctg catccacttc tcgtgctcgg ccaggtcggg
      781 gcagccgtgc acggggtggg cgcgcagctc gtcccggagg gcggccagct ccggatcgtc
      841 ggcgccgggc cgccggcggc gccggggccg ggggaccgaa ggcgggtcga gctcggccag
      901 ggccctggcc acgtacgact tgaaccgggg gctcttgggg ctgagctggc ggggcatggc
      961 caccttgccg acccgcaccg ggatgccgcc caggtcgcgg acggcgaccc ggcggacccg
     1021 gcggtcctcg gtcaggacca ggatcccggt cgagttgtcg cggccgccgg ccggcccgat
     1081 gaccgcggcc agcccgcggc ggcgcccgcc gggcagccag atgacggtgc cgggggtcag
     1141 cgagcgcagc gcctcggccg cctcctgggt ctcggcccgg cggtcggcgt cggccagggt
     1201 gctctcgcgg gcccgcagcc gccggcgcag ctcccagtac ccggtgatgt cgccccggtc
     1261 gcaggccgcc gactccaggt agccggcgag gaagcgggcg ttgcgggcca tggtctgctc
     1321 ggccccgtgc acggtccggt cggccaggaa ctgggcgaac gaggaggcca gcagccgctc
     1381 ggcctgcccc cgctcgtagt tggcgaccag gttgacggcc atgttgtagg agggacggaa
     1441 cgacgaggtg agcgggtagg tgcgggtcga ggccagcccg gtgatccgct cgaaggggat
     1501 gtagggctgg tggagcacga ccgcggctcc gcggctgtcg atcccgcgcc ggccggcccg
     1561 ccccgtgagc tgggtgtact cgcccggggt gagcagctcg tggcgctcgc cctgccactt
     1621 ggtgagccgc tcgatggcca ccgtccgggc cggcatgttg atccccagcg acagcgtctc
     1681 ggtggcgaac acgaccttga ccagtccctg ggcgaacagc tcctcgaccg cctccttgaa
     1741 caggggcagc attccggcgt ggtggctggc cacgccgcgg cccagcgact cggccaggag
     1801 gtcgtagccg agcgcgtcca ggtcggccgg gtcgatgggg gccaccttga gctcgaggaa
     1861 ctcgaggatc tcggcccgct cggcgtggct ggtgagccgg atgttgccgg cccggcactg
     1921 ctcgagggcc tggtcgcagc cggcccggga gaagatgaag tagatggccg ggagcagccg
     1981 ctccgcctcc agcaggtcga ccacctcgac ccgggacggc acccagcccc gacctaccgg
     2041 cctcggtccg ttgcgcccgg gacggccctt ggtgtcgcgg cccggccgcc actcgcgctc
     2101 gccccgacgg gccagggccg ggttcgggac cacgtcgtcg cccctggtca cgaacatcgg
     2161 gtgcagctcg cggccgatca ggtagtggtt gtcgaggggg acggggcggc gctcctcgat
     2221 caccacctcg gtggcgccgc gggtcgcctc cagccactca ccgaactcct cggcgttgga
     2281 gacggtggcc gagaggctgg ccacccggac ctcgaccggg aggttgatca ggacctcctc
     2341 ccagaccgcc ccccggaccc ggtcctgaag gtagtggacc tcgtccatga ccacatagcg
     2401 caggccgccg agggtggggc tgccctcgta gagcatgttg cggagcacct
//
LOCUS       DASSWR010000274         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_971038,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000274 DASSWR010000000
VERSION     DASSWR010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971038"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..457
                     /locus_tag="VFO47_04600"
     CDS             209..457
                     /locus_tag="VFO47_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET8788007.1"
                     /translation="MPVYEYACRACGASEEHLQPLGSDAPGPCQACGGELRRRWSRVA
                     VRYQGWGFTSTDKLVGDDRRDQDFRALRDRAERIADGE"
     gene            complement(461..808)
                     /locus_tag="VFO47_04605"
     CDS             complement(461..808)
                     /locus_tag="VFO47_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788008.1"
                     /translation="MRRSWVRLGLLAAVVVAVVLVALGRGDVVVALLLGGLVLATGRA
                     VFRGYSTTKGPTGPPVDVAEEGTLLVFACETCGEQLVLLRKGTDAPPRHCAEKMVLRR
                     VPTPSSDPDPRFN"
     gene            complement(816..2018)
                     /locus_tag="VFO47_04610"
     CDS             complement(816..2018)
                     /locus_tag="VFO47_04610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET8788009.1"
                     /translation="MQARAQEPGLEPVVVGAVRTPVGKRNGALAGVHAVDLGAVVLRA
                     LVERSGVDPVLVDDVIMGCVSQVGEQSLNVARNAWLAAGLPEEVPATTIDRQCGSSQQ
                     AVQFAAQGIMAGGYGLVVAGGVEHMTRVPMGSTMSGPGVPFGPAMHDRYQGRLVPQGI
                     SAELITEKWGLTREEQDEFALRSHRRAAAAQDAGRFDRQLVPVEVPGPDGDGGGGPTL
                     VRADEGVRRDTSMERLAGLKPAFRPDGQVTAGNSSQISDGAAALLLASRETAERLGLR
                     PLARFAGFALAGVDPVLMLTGVIPATERVLRQTGLGMDDLDVIEINEAFASVVLSWGR
                     EVEPNWDRVNPNGGAIALGHPLGASGARIMTDLVAELERGGGRYGLQVMCEGGGMANA
                     TVLERLDG"
     gene            complement(2101..>2406)
                     /locus_tag="VFO47_04615"
     CDS             complement(2101..>2406)
                     /locus_tag="VFO47_04615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8788010.1"
                     /translation="GDPLGNGRGGPGYQFEDEVDGPHRFDRPGYLAMANAGPNTNGSQ
                     FFITQVPTPWLDGTHTIFGEVVEGMEVVDAMAAVDRDPTDRPVEELRLERVTIEQAE"
BASE COUNT          318 a          953 c          814 g          321 t
ORIGIN      
        1 gctcttctac atgcagatcc tgccgtcgtt cctcgacgac ctggggaaca tcaacctggc
       61 catcgggttc gtgcccctca ttgtcgggct gctgctggcc acgcgctgga cctgagcagg
      121 tcgagcggct gacctgccga actacacctc tgtcatttct tccccagctg gggataaggc
      181 tggggacaac ccgccgggag gcctgtggat gcccgtctac gagtatgcct gccgggcctg
      241 cggcgcctcc gaggagcatc tccagccgct cggcagcgac gcccccggcc cctgccaggc
      301 ctgcggcggc gagctgcgcc ggcgctggtc ccgggtggcc gtcaggtacc agggctgggg
      361 cttcaccagc accgacaagc tggtcggcga cgaccgccgc gaccaggact tccgcgccct
      421 ccgcgaccgc gccgaacgca tcgccgacgg cgagtgagcc tcagttgaac cgcgggtccg
      481 ggtcgctcga cggggtgggg acgcggcgca ggaccatctt ctcggcgcag tggcggggcg
      541 gcgcgtcggt gcccttgcgc aggaggacca gctgctcccc gcaggtctcg caggcgaaaa
      601 cgagcagggt cccctcctcg gcgacgtcca ccggcggccc ggtcggcccc ttggtggtcg
      661 agtagccccg gaacaccgcc cgcccggtgg ccagcaccag cccaccgagc agcagcgcca
      721 cgaccacgtc cccccgcccc agcgcgacca gtaccacggc cacgaccacc gcggccagca
      781 gccccagccg tacccagctc cgccgcaccg gggccctacc cgtccagccg ttcgagcacg
      841 gtcgcgttgg ccatgccgcc gccctcgcac atcacctgga ggccgtagcg gccgcccccg
      901 cgctccagct cggcgaccag gtcggtcatg atccgggcgc cggaggcccc gaggggatgc
      961 ccgagggcga tggccccgcc gttcggattc acccggtccc agttcggctc gacctcgcgg
     1021 ccccaggaca ggacgacgct cgcgaacgcc tcgttgatct cgatgacgtc caggtcgtcc
     1081 atgcccaggc cggtctgccg cagcacccgc tcggtggccg ggatcacccc ggtgagcatc
     1141 agcaccgggt cgaccccggc aagggcgaac cccgcgaacc gggccagggg ccgcagcccc
     1201 agccgctcgg ctgtctcccg gctggccagc agcagcgcgg ccgccccgtc cgagatctgg
     1261 ctggagttcc ccgccgtcac ctgcccatcc ggccggaagg cgggcttcag cccggcgagc
     1321 ctctccatgg aggtgtcccg ccgcaccccc tcgtcggccc gcaccaaggt cgggccgccg
     1381 cccccgtccc cgtccggccc ggggacctcg accgggacca gctgacggtc gaagcggccg
     1441 gcgtcctggg cggcggccgc ccgccggtgg gagcggaggg cgaactcgtc ctgctcctcg
     1501 cgggtcaggc cccacttctc ggtgatcagc tcggccgaga tgccctgggg gaccaggcgt
     1561 ccctggtagc ggtcgtgcat ggccggcccg aagggcaccc cgggaccgct catggtcgag
     1621 cccatcggca cccgggtcat gtgctcgacc cctccggcca cgaccagccc gtagccgccg
     1681 gccatgatcc cctgggcggc gaactggacc gcctgctggg aggagccaca ctgccggtcg
     1741 atggtggtcg ccggcacctc ctcgggcagc ccggcggcca gccaggcgtt gcgggccacg
     1801 ttcagcgact gctccccgac ctgggacacg cagcccatga tcacgtcgtc gaccagcacc
     1861 ggatcgaccc cgctgcgctc gaccagcgcc cgcagcacca ccgcccccag gtcgacggca
     1921 tgcaccccgg ccagcgcccc gttgcgcttc cccaccggcg tccgcaccgc cccgacgaca
     1981 accggctcca gccccggctc ctgtgcccgc gcctgcatgt gtgccccttc cgcagtcctg
     2041 ctgcagcgaa ggtggcactg cccgccgccc tcgtcaacca ggcggccgac ccggagttgg
     2101 ctactcggcc tgctcgatgg tcacgcgctc gagccgcagc tcctcgaccg ggcggtcggt
     2161 cgggtcgcgg tcgacggcgg ccatggcgtc gacgacctcc atgccctcga cgacctcgcc
     2221 gaagatggtg tgcgtgccgt cgagccaggg tgtcgggacc tgagtgatga agaactggga
     2281 cccgttggtg ttgggcccgg cgttggccat ggccaggtag cccgggcggt cgaagcggtg
     2341 ggggccgtcg acctcgtcct cgaactggta gccggggccg ccgcgcccgt tgccgagcgg
     2401 gtcgcc
//
LOCUS       DASSWR010000275         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_971039,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000275 DASSWR010000000
VERSION     DASSWR010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971039"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFO47_04620"
     CDS             <1..442
                     /locus_tag="VFO47_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET8788011.1"
                     /translation="PPAGPTWPQAPPPARDYGRQGYTQPYGGQQGYGQPPYGQQPGYG
                     PPYGAPPGAGYGYTQAPPTDSSATAALVIAIIGFFTCPPVGAIVALVLANSAQKRIEA
                     SNGQLGGLEMARAARIIAIIELALTALALVGGLIAIAVSLGSTY"
     gene            460..819
                     /locus_tag="VFO47_04625"
     CDS             460..819
                     /locus_tag="VFO47_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET8788012.1"
                     /translation="MPTRPRRPLAMLLLVLSFTLAACQAGSAASPADAAPVRGVTTVD
                     AKDLKFLPPAIEVPPGTEVTWRFVDGSVPHNVKGDGFASENQSRGTFSHRFEQAGEYR
                     YTCDLHAGMDGRVVVAG"
     gene            852..1586
                     /locus_tag="VFO47_04630"
     CDS             852..1586
                     /locus_tag="VFO47_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET8788013.1"
                     /translation="MADEATLTPAIQEYLESLYWLGEAGIERTPTNLSRAMQVSPPSV
                     TGMVRRLESEGLVDRGPGKRIVLTDQGMEVAKHVVSRHRLVEAFLVKVMGVPWDEVHE
                     EAEAFEMGVTPALEARMLEMIGEARTCPHGHPIGDYPREPGVPLTAVPIGSVVRVLRF
                     ENEDHDLLRMFRRAGVEPGASYTIAAFGQERAELEGPQGATVLPLYAARTISVAVERS
                     GDGPAAEQDPESLATAYLLGETHWSR"
     gene            complement(1606..1736)
                     /locus_tag="VFO47_04635"
                     /pseudo
     CDS             complement(1606..1736)
                     /locus_tag="VFO47_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957143.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
     gene            complement(1862..>2412)
                     /locus_tag="VFO47_04640"
     CDS             complement(1862..>2412)
                     /locus_tag="VFO47_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET8788014.1"
                     /translation="ALENLFPPIPSEVILPLAGFTASQGDMSLAAALAWTTFGSVAGA
                     VVLYWIGALIGRERVRAIAARLPLIKVSDIDRTEAWFERHGRKTVFLGRMVPLFRSFV
                     SIPAGIERLPMTSFLLLTTLGSLIWNTLLVMAGYVLGENRRIIEDYAGTYQNVVLAVG
                     FLAVAGFVVVRLVAARRNRQRV"
BASE COUNT          359 a          909 c          850 g          294 t
ORIGIN      
        1 cccgccggcc gggccgacct ggccgcaggc cccgccgccc gcccgggact acggccggca
       61 gggctacacg cagccctacg gcggccagca aggctacggc cagcccccct acgggcagca
      121 gcccggctac gggcccccct acggcgcgcc cccgggcgcc gggtacggct acacgcaggc
      181 gcccccgacc gacagctcgg cgacggcggc gctggtgatc gccatcatcg gcttcttcac
      241 ctgcccgccc gtcggtgcca tcgtcgccct ggtgctggcc aacagcgccc agaagcgcat
      301 cgaggcctcc aacggccagc tcggcggcct ggagatggcc agggcggccc gcatcatcgc
      361 catcatcgag ctcgcactca ccgccctcgc gctcgtgggc ggcctgatcg ccatcgcggt
      421 ctcgctcggc tccacctact gacccgtcaa ggagcttccg tgccgacccg cccccgccgc
      481 cccctcgcca tgctcctgct tgtgctctcg ttcacattgg ccgcctgcca ggccggctcg
      541 gccgcctccc ccgccgacgc cgcgccggta cggggcgtga ccacggtcga cgccaaggac
      601 ctcaagttcc tgcccccggc gatcgaggtg ccgccgggca cggaggtcac ctggcggttc
      661 gtggacggct cggtccccca caacgtcaag ggggacggct tcgcctccga gaaccagtcc
      721 cgggggacct tcagccaccg gttcgagcag gccggtgagt accgctacac ctgcgacctg
      781 cacgccggaa tggacggccg ggtggtggtg gccggctgac cggaaccggc ggcgccacgt
      841 accatgggcc catggccgac gaagcgaccc tcaccccggc gatccaggag tacctggagt
      901 cgctctactg gctcggcgag gccggcatcg agcggacgcc gaccaacctg tccagggcca
      961 tgcaggtgtc gccgcccagc gtcaccggga tggtccggcg gctcgagagc gagggcctgg
     1021 tcgaccgggg accgggcaag cggatcgtcc tcaccgacca gggcatggag gtggccaagc
     1081 acgtggtcag ccggcaccgg ctggtcgagg ccttcctggt caaggtgatg ggggtgccct
     1141 gggacgaggt ccacgaggag gccgaggcct tcgagatggg cgtcaccccg gcgctcgagg
     1201 cccgcatgct cgagatgatc ggcgaggccc ggacctgccc gcacggccac ccgatcggcg
     1261 actacccgcg ggagccgggc gtgcccctca ccgccgtccc catcggcagc gtcgtccgcg
     1321 ttctcagatt cgagaacgag gaccacgacc tgctgcggat gttccgccgg gccggggtcg
     1381 agccgggggc cagctacacc atcgccgcct tcggtcagga gcgggccgag ctggagggcc
     1441 cgcagggagc gaccgtgctg cccctgtacg cggccaggac gatctcggtg gcggtcgagc
     1501 gctcgggcga cggccccgcg gccgagcagg accccgagag cctggccacg gcctacctgc
     1561 tcggcgagac ccactggagc cggtagccgg ccgggacccg tccgcgggct cgtggaccgg
     1621 gtcggtggcg taggtctggc cgtaggcgtg caagccgtgg cgggcacctt gtcggctggg
     1681 ctgttgacgg ccgtccagcg cccggcaacc ggggccgcga cctcgaccgg tgggcggcgc
     1741 acggtcccga cccggaagta cagggccagc ccggcgacca gcagcaccag ggcgccccac
     1801 agcagcggca tccggaggcg ggcggcgtac acggggggtc gcgccctcca ggtccccacg
     1861 gctagacgcg ctggcggttg cgacgggccg cgaccaggcg gacgaccacg aagccggcca
     1921 cggcgaggaa cccgacggcg agcacgacgt tctggtaggt cccggcgtag tcctcgatga
     1981 tccggcggtt ctccccgagg acgtagccgg ccatcaccaa cagggtgttc cagatcaggc
     2041 tgcccagggt ggtgaggagc aggaaggacg tcatcggcaa ccgctcgatc cccgccggga
     2101 tcgagacgaa gctgcggaac agcggcacca tgcggccgag gaacaccgtc ttgcgcccgt
     2161 gccgttcgaa ccacgcctcg gtccggtcga tgtcggacac cttgatgagc ggcagccgag
     2221 cggcgatggc cctgacgcgc tcccgcccga tcagcgcccc aatccagtac agcaccaccg
     2281 ccccggccac cgacccgaag gtggtccagg cgagggccgc ggccaggctc atgtccccct
     2341 ggctggccgt gaacccggcc agcggcagga tcacctcact gggaatcggc gggaacaggt
     2401 tctccagcgc ca
//
LOCUS       DASSWR010000276         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_8860,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000276 DASSWR010000000
VERSION     DASSWR010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8860"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1359
                     /locus_tag="VFO47_04645"
     CDS             49..1359
                     /locus_tag="VFO47_04645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET8788015.1"
                     /translation="MNQGARPTGEVVAGDRPLEPGEADGLGILHADLDAFYASVEVLK
                     DPSLRGRPLLVGGTGARGVVTSASYEARAFGCRNAMPMARARRLCPQAVAVPPDFAAY
                     RRHSATVMRIFQSLTPLVEPLALDEAFLDVRGARALFGDAVATARLLRARVREEAGLA
                     LTVGVAASKYLAKLASTRGKPDGLLVVPPGRALEFLHPLPVDALWGAGQATIETLARY
                     GLRTVGDVAATPRATLERALGPAAGAQLHELAWARDDREVVPFEAAKSVGSEETFTTD
                     IDDPGQLAREVLRSCVRVGRRLREAGLAGRTVTLKLRFADFRTITRGRTLPVATDTDT
                     ELHQVARELLARLQLGRTPVRLVGVTVSNLQRQGAPVQLRLGPERPGWEAAVRAADAV
                     RARFGDGAIDLASLTGDRPAGAYEEARQTPRPRRDPLLPPDPEP"
     gene            1484..1921
                     /locus_tag="VFO47_04650"
     CDS             1484..1921
                     /locus_tag="VFO47_04650"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HET8788016.1"
                     /translation="MPLSEHEQRILAEIERRLLEEDPKFAHQVGSSFRAHLGRRLKLA
                     VAGFVLGLVVVISSTFLENVAVGVAGFVIMLASVFVFVRTMRRRATVERPVSGAPPPS
                     GPAPTGQRRKGGGGPKGGIDAWWGRMTERWRSRWEERGGQGPP"
     gene            complement(1937..>2218)
                     /locus_tag="VFO47_04655"
     CDS             complement(1937..>2218)
                     /locus_tag="VFO47_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788017.1"
                     /translation="DDFAADADPLVELAGLFERAEYAPAEPDGDQAAAARRLARSARA
                     GLAGRLGWRRRLLAAVSPRSLVAGRAAVPRETSREAPSRERELVGQARL"
BASE COUNT          258 a          873 c          812 g          275 t
ORIGIN      
        1 catcctagtc ggatgcacct tccagagggg tgtccaggga gtggtccggt gaaccaggga
       61 gcgcggccga ccggcgaggt ggtcgccggg gaccggccgc tggagccggg tgaggccgac
      121 ggcctgggga tcctccacgc cgacctggac gccttctacg ccagcgtcga ggtgctcaag
      181 gacccgtccc tgcgcggccg tcccctgctg gtcggcggca ccggcgcccg cggggtcgtc
      241 acctcggcct cgtacgaggc cagggcgttc ggctgccgca acgccatgcc gatggccagg
      301 gcccgccgcc tgtgcccgca ggcggtggcc gtcccgcccg acttcgccgc ctaccggcgc
      361 cactcggcca cggtcatgcg gatcttccag agcctcaccc cgctggtcga gccgctcgcc
      421 ctcgacgagg cgttcctgga cgtccgcggg gcccgggcgc tgttcggcga cgccgtcgcc
      481 acagcgcggc tgctccgggc ccgggtgcgg gaggaggccg gcctggccct gacggtcggc
      541 gtggccgcca gcaagtacct ggccaagctg gcctccaccc gcggcaagcc ggacggcctg
      601 ctggtggtcc cgcccggccg ggccctggag ttcctccacc ccctgccggt cgacgccctc
      661 tggggggccg gccaggccac catcgagacg ctggcccgct acggcctgcg caccgtcggc
      721 gacgtcgccg ccaccccgag ggcgaccctg gagcgggccc tcgggccggc cgccggggcc
      781 cagctccacg agctggcctg ggcccgcgac gaccgcgagg tggtgccgtt cgaggcggcc
      841 aagtcggtcg ggtccgagga gaccttcacc accgacatcg acgaccccgg gcagctggcc
      901 cgcgaggtgc tgcgctcctg cgtgcgggtg ggacggcggc tgcgggaggc gggcctggcc
      961 ggccgcacgg tcaccctgaa gctccgcttc gccgacttcc gcaccatcac ccggggccgc
     1021 accctgccgg tggccaccga caccgacacc gagctgcacc aggtcgcccg ggagctgctg
     1081 gcccggctcc agctcggccg gaccccggtc cgcctggtcg gtgtcaccgt cagcaacctg
     1141 cagcgccaag gcgcgccggt gcagctgcgg ctgggcccgg agcggcccgg ctgggaggcg
     1201 gcggtccggg ccgccgacgc ggtcagggcc cggttcggcg atggcgcgat cgacctggcc
     1261 tcgctcaccg gcgaccgccc ggccggggcc tacgaagagg cccgccagac cccccgcccc
     1321 cgccgcgacc ccctcctccc cccggacccc gaaccgtgac ccgagattga aggtttagga
     1381 ccgcgcgtga gtccccacca aagttccaag tggagtcgcc ggctgggcct cagccgggta
     1441 cactcgcagg accaccacag tacgaccctg gccggagcct catatgccgc tgtccgagca
     1501 cgagcaacgc atcctggccg agatcgagcg gcggttgctc gaagaggacc ccaaattcgc
     1561 ccatcaggtg ggctcctcgt tccgtgccca tctgggccgc cgcctcaagc tcgccgtcgc
     1621 cggcttcgtc ctcggcctgg tcgtggtcat ctcctccacg ttcctggaga acgtcgccgt
     1681 cggcgtggcc ggcttcgtca tcatgctggc ctcggtgttc gtgttcgtgc ggacgatgcg
     1741 ccggcgggcc acggtggagc gcccggtgag cggcgcgccc ccgccgtccg ggccggcccc
     1801 caccggccag cgccgcaagg gcgggggcgg ccccaagggc ggcatcgacg cctggtgggg
     1861 acggatgacc gaacgctggc gctcccgctg ggaggagcgc ggcggccagg gacctccctg
     1921 accggctgac ccttctctac aggcgggcct ggccgaccag ctcccgctcc ctcgacggcg
     1981 cctcccgtga cgtctcccgg gggacggccg cgcgcccggc gaccaggctg cggggactga
     2041 ccgcggccag caggcgccgg cgccagccga ggcggccggc caggccggcc cgggcgctgc
     2101 gggccaggcg cctggccgcg gcggcctggt cgccgtccgg ctcggccggg gcgtactcgg
     2161 cccgctcgaa caggccggcc agctcgacca gcgggtcggc gtcggcggcg aagtcgtc
//
LOCUS       DASSWR010000277         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_744545,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000277 DASSWR010000000
VERSION     DASSWR010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744545"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFO47_04660"
     CDS             <1..595
                     /locus_tag="VFO47_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8788018.1"
                     /translation="QVCISVQRVIAHQAVVGDFLDALVPKVEAIRTGDPGSPDTGMGA
                     LITAGEAERVRKAIAEAAAAGARVLTGGDREGAVVQPAVVADVDPASPFSQEELFGPA
                     VAVSRADGWAEAIAQANGTGYGLGSGIFTADVAGAVQAMREIDSGTIHVNWTPLWRAD
                     LMPYGGLKGSGIGKEGPRSAVEEMTEVKTVVLHGRPW"
     gene            622..2583
                     /gene="iolD"
                     /locus_tag="VFO47_04665"
     CDS             622..2583
                     /gene="iolD"
                     /locus_tag="VFO47_04665"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET8788019.1"
                     /translation="MTASDRRTVRLTVAQAIVRYLAAQHSVADGDRRRLIPATLGIFG
                     HGNVAGMGQALDQLHDRLPFIQGRNEQALVHAATAFAKATRRRATLAVTASIGPGALN
                     MVTGAGVATVNRLPVLLLPGDAYATRRQGPVLQQLQHPTEADVSANDAFRPLARFFDR
                     ITRVEQLLTALPEAMRILTDPAATGAVVLALPQDIQSHAYDYPAGLFEEHDWPIRRPP
                     PDPREVAEVVRLLSRARRPLLIAGGGVVYSDASRELEAFARATGIPVAETFAGKGAVQ
                     DGDWWLLGGIGLEGTPATNALARQADLVLTVGSRLTDFATASQSLFEHPEVRFASVNV
                     DGHDAARLGATPIIGDARLALAALTEAAREAGVASPEAWREEVRAQTGAWATARAAAL
                     DPDTTFDRSTVQGDVIPDTGAELTQGQIIGLLQEHAQPGDTIIAAAGGPPGDLQKVWD
                     ATGGRHCHLEFGFSCMGYEVPAAIGVRLAEPDPARRVLSFVGDGTFLMAPTELVTAAQ
                     EGLAVTVVIPENHGYQVIHRLQMLRSGREFGNEFRYRKDGLDLAADATQSRLEGDYLK
                     VDLAQVAAGLGARAVLARTAKEVRDALADTRDHPGPVVIVVPAIPHADLPGAGVWWDV
                     APAEVSETPGVAELRAEYEQGLELQRWHG"
     gene            2580..>2797
                     /locus_tag="VFO47_04670"
     CDS             2580..>2797
                     /locus_tag="VFO47_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET8788020.1"
                     /translation="MTATGTVPARTPLDAGRLRARLAAGEATVGTFLGMASPVAAEVC
                     AAAGPDWVLLDLEHGGGGEEQVGATVLAA"
BASE COUNT          349 a         1115 c         1001 g          332 t
ORIGIN      
        1 ccaggtctgc atctcggtcc agcgggtcat cgcccaccag gcggtggtcg gcgacttcct
       61 ggacgccctg gtgcccaagg tcgaggccat ccgcaccggc gacccgggct cgcccgacac
      121 cggcatgggc gcgctcatca ccgccggcga ggccgagcgt gtgcgcaagg ccatcgccga
      181 ggcggccgcc gccggggccc gcgtgctcac cggcggggac cgcgaggggg cggtggtgca
      241 gccggccgtg gtggccgacg tcgacccggc gtcgcccttc agccaggagg agctgttcgg
      301 cccggcggtg gccgtcagcc gggccgacgg ctgggccgag gcgatcgccc aggccaacgg
      361 gaccggctac gggctcgggt cggggatctt caccgccgat gtggccgggg ccgtccaggc
      421 catgcgcgag atcgactcgg ggaccatcca cgtcaactgg accccgctgt ggcgggccga
      481 cctgatgccc tatggcggcc tcaagggcag cggcatcggc aaggagggcc cccgctccgc
      541 cgtcgaggag atgaccgagg tcaagacggt cgtgctccac ggccgtccct ggtgaccaac
      601 cgtttgaagg gagatcgacc catgaccgct tccgaccggc ggaccgtgcg cctgacggtc
      661 gcccaggcca tcgtcaggta cctcgccgcc cagcactcgg tggccgacgg cgaccgccgc
      721 cggctcatcc cggccaccct cggcatcttc ggccacggca acgtcgccgg catgggccag
      781 gccctcgacc agctccacga ccggctgccg ttcatccagg gccgcaacga gcaggccctg
      841 gtccacgcgg ccaccgcctt cgccaaggcg acccgccgcc gggccaccct ggccgtcacc
      901 gcctcgatcg gccccggggc gctcaacatg gtcaccgggg ccggggtggc caccgtcaac
      961 cggctcccgg tgctgctgct ccccggcgac gcctacgcga cccggcgcca gggcccggtg
     1021 ctgcagcagc tccagcaccc gaccgaggcc gacgtcagcg ccaacgacgc cttccggccc
     1081 ctggcccggt tcttcgaccg catcacccgt gtcgagcagc tgctgaccgc cctgcccgag
     1141 gccatgcgga tcctgaccga cccggcggcc accggcgccg tggtcctggc cctgccccag
     1201 gacatccagt cgcacgccta cgactacccg gccgggctgt tcgaggagca cgactggccc
     1261 atccgccggc cgccgcccga cccccgggag gtggccgagg tcgtgcgatt gctgtcgcgg
     1321 gcccgccggc cgctgctgat cgccggcggc ggggtcgtct actcggacgc ctcccgcgag
     1381 ctggaggcct tcgcccgcgc caccggcatc cccgtggccg agaccttcgc cggcaagggc
     1441 gcggtccagg acggcgactg gtggctgctg ggcggcatcg ggctggaggg caccccggcc
     1501 accaacgccc tggcccgcca ggccgacctc gtcctcaccg tcggctcccg cctgaccgac
     1561 ttcgccaccg cctcccagtc gctgttcgag caccccgagg tgcggttcgc ctcggtcaac
     1621 gtggacggcc acgacgcggc ccggctcggc gccaccccga tcatcgggga cgcccgcctc
     1681 gccctggccg ccctcaccga ggccgcccgg gaggccgggg tggccagccc cgaggcgtgg
     1741 cgggaggagg tccgggccca gaccggcgcc tgggcgacgg cccgggcggc cgccctcgac
     1801 cccgacacca ccttcgaccg ctccacggtc cagggcgacg tgatccccga caccggcgcc
     1861 gagctgaccc aggggcagat catcggcctg ctccaggagc acgcccagcc gggcgacacg
     1921 atcatcgccg ccgccggcgg cccccccggc gacctccaga aggtctggga cgccaccggc
     1981 ggccgccact gccacctgga gttcggcttc tcctgcatgg gctacgaggt gccggcggcc
     2041 atcggggtcc gcctggccga gcccgacccg gcccgccggg tgctctcgtt cgtcggcgac
     2101 ggcaccttcc tgatggcccc gaccgagctg gtcacggccg cccaggaggg gctggcggtg
     2161 accgtggtca tccccgagaa ccacggctac caggtcatcc accgcctcca gatgctgcgc
     2221 agcgggcgcg agttcggcaa cgagttccgc taccgcaagg acggcctgga cctggccgcc
     2281 gatgccaccc agtcgcggct cgagggcgac tacctcaagg tcgacctggc ccaggtggcc
     2341 gccggcctgg gggcccgggc ggtcctggcc cggacggcca aggaggtccg cgacgccctc
     2401 gccgacaccc gcgaccatcc cggcccggtg gtgatcgtgg tcccggccat cccccatgcc
     2461 gacctgcccg gcgccggcgt ctggtgggac gtggcccccg ccgaggtgtc cgagacgccg
     2521 ggggtggccg agctgcgggc cgaatacgag caggggctgg agctccagcg gtggcacgga
     2581 tgacggccac cggcaccgtc ccggcgcgga cgccgctgga cgccggccgg ctgcgggccc
     2641 gcctggccgc cggcgaggcg accgtcggga ccttcctcgg catggcctcg ccggtggcgg
     2701 ccgaggtctg cgccgccgcc gggcccgact gggtgctgct ggacctggag cacggcggcg
     2761 gcggtgagga gcaggtcggg gccaccgtcc tggccgc
//
LOCUS       DASSWR010000278         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_38706,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000278 DASSWR010000000
VERSION     DASSWR010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38706"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VFO47_04675"
     CDS             <1..178
                     /locus_tag="VFO47_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8788021.1"
                     /translation="DPELGEIAMQNVAPRLSATPGRVAWPGPALGQHNQEVYQGLLGL
                     PDEEVERLADQGVI"
     gene            213..2276
                     /locus_tag="VFO47_04680"
     CDS             213..2276
                     /locus_tag="VFO47_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8788022.1"
                     /translation="MPYRLGVDVGGTFTDLLMIDEDSGETWRAKTPSTPADPSIGVLA
                     GLEKVCQTASVDPGAISHLMHGTTVATNAILESKGARVGLVVTEGYRQVLQVARSFVP
                     GGLAGWIIWPKPEPLAALEHTVEVAERVGARGEVLRPLDEASVRAALAELRRRQVEAL
                     TVSLVNSFANPAHERRILELAAEELPGVPVSLSSDILPEIREYERTLTTVANSYVRPV
                     VSRYLGNLERELGDRQVGARLHVLRSDGGLMSVEAAKQAPVNMLMSGPAGGVTGALWI
                     AEKAGFDNLLTFDMGGTSTDVALVQQGRPRVGRETAVGDLNVRASSVDVHTVGAGGGS
                     IAHVPELTRALRVGPQSAGAEPGPAAYGRGGAEPTVTDANVVLGYLPPSLLGGEMSLD
                     AAAAEAAVAKVAEAMGLETRRAAAGIVDIVNENMFGALRLVSVQQGYDPREFALVAFG
                     GAGPLHANALGRLLGSWPVIIPPSPGVLCAYGDATTRVRNESARTFIRRFSETSNEEV
                     ERLLAELAAEAAAALDGDGVGPDQQSVLYQVDVRYHGQGFEVPVDVELERFRDGGLEH
                     VGQVFDVEHTRLFTFALDTEHELVNLRAVVQGRATRVRAEEVPAGGSDPSAAATGTTT
                     IWVGDGERQATVYDRARLEAGNRVQGPAVVTEMDSTTLVLPDHVGEVDRYGNLLLRPA
                     SNEGS"
     gene            2280..>3651
                     /locus_tag="VFO47_04685"
     CDS             2280..>3651
                     /locus_tag="VFO47_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8788023.1"
                     /translation="MATVIETNPRQFAPVEVDPVTVDIIENALRNTRYEMDAVLFRTA
                     MSPGIREQHDEFPIIADRAGRMVVGQFGSFIDGFLKTYQGEISEGDVFLTSDPYACDG
                     AISHANDWLVLLPIYREGALVGWAAMFGHMTDVGGKVPGSLPTDATSIYEEGVVVPPT
                     KLYRKGELATEALELVLNQVRMKTWNRSDLNAIVAACRTAARRVLELCDRFGTETYLS
                     TLDALLDRNYRAMAQLIRTAVPTERLEFTDYVCDDGRGYGPYKIHCSMQRVGDKVVLD
                     FDGTDPQAEGSINFYLNENMLKMFFGIYMIMVFDPQILWNDGFYPLVEVKIPEGSLLK
                     PRYPAALSCRTHALGRIFDVMGGLLGQRAPEFLCAAGFSSSPHLMYSGVDRNGEWFQL
                     YQIGFGGIPGRPFGDGPDGHSLWPSFTNVPNEFLESYFPLVIERYQTVADSGGPGFFR
                     GGNGIDI"
BASE COUNT          519 a         1381 c         1253 g          498 t
ORIGIN      
        1 cgacccggag ctgggggaga tcgccatgca gaacgtggcc ccgcggctgt cggccacccc
       61 aggccgggtc gcctggccgg ggccggccct cggccagcac aaccaggagg tgtaccaggg
      121 gctgctcggc ctgcccgacg aggaggtcga gcgcctggcc gaccagggag tgatctaggc
      181 gccggcccgg cgccgcgatg aggaggcaca ccatgcccta ccggctcggg gtggacgtgg
      241 gcggcacgtt caccgacctg ctgatgatcg acgaggacag cggcgagacc tggcgggcca
      301 agaccccgtc gaccccggcc gacccgtcga tcggggtgct ggccggcctg gagaaggtct
      361 gccagacggc gtcggtcgac cccggcgcca tcagccacct gatgcacggg accacggtgg
      421 ccaccaacgc catcctggag agcaagggcg cccgggtcgg cctggtcgtc accgagggct
      481 accggcaggt cctccaggtc gcccgctcgt tcgtccccgg cgggctggcc ggctggatca
      541 tctggcccaa gccggagccg ctggccgccc tcgagcacac cgtcgaggtg gccgagcggg
      601 tcggggcccg cggcgaggtg ctgcgcccgc tggacgaggc cagcgtccgg gccgccctgg
      661 ccgagctgcg ccgccgccag gtcgaggccc tgaccgtgtc gctggtcaac tccttcgcca
      721 acccggccca cgagcggcgc atcctggagc tggcggccga ggagctgccc ggtgtgccgg
      781 tgtcgctctc ctccgacatc ctccccgaga tccgcgagta cgagcgcacc ctgaccaccg
      841 tggccaactc ctacgtgcgc ccggtcgtct cccgctacct gggcaacctg gagcgggagc
      901 tgggtgaccg ccaggtcggg gcccggctgc acgtgctccg ctccgacggc gggctgatga
      961 gcgtcgaggc ggccaagcag gccccggtca acatgctgat gagcggcccg gccgggggcg
     1021 tcaccggcgc cctgtggatc gccgagaagg ccggcttcga caacctgctc accttcgaca
     1081 tgggcgggac ctcgaccgac gtcgccctag tccagcaggg ccgcccccgg gtggggcgcg
     1141 agacggccgt cggcgatctc aacgtccgcg cctcctcggt cgacgtccac accgtgggcg
     1201 ccggcggcgg ctcgatcgcg cacgtgcccg agctgacccg ggccctgcgg gtcgggcccc
     1261 agagcgccgg ggccgagccc ggcccggccg cctacggccg cggcggcgcc gagcccaccg
     1321 tcaccgacgc caacgtggtc ctcggctacc tgccgccgtc gctgctgggc ggcgagatga
     1381 gcctggacgc ggccgccgcc gaggccgccg tggccaaggt ggccgaggcc atgggcctgg
     1441 agacccgccg ggccgcggcc ggcatcgtcg acatcgtcaa cgagaacatg ttcggcgccc
     1501 tgcggctcgt ctcggtgcag cagggctacg acccgcgcga gttcgccctg gtcgccttcg
     1561 gcggggccgg gccgctgcac gccaacgccc ttggccgcct gctcggctcc tggccggtga
     1621 tcatcccccc gtccccggga gtgctgtgcg cctacgggga cgccaccacc cgggtccgca
     1681 acgagtcggc ccggaccttc atccggcggt tctccgagac ctccaacgag gaggtggagc
     1741 ggctcctggc cgaactggcc gccgaggcgg cggccgccct cgacggcgac ggggtcggcc
     1801 ccgaccagca gtcggtcctg taccaggtgg acgtccgcta ccacggccag gggttcgagg
     1861 tcccggtcga cgtcgagctg gagcgcttcc gcgacggcgg gctggagcac gtcggccagg
     1921 tgttcgacgt cgagcacacc cggctgttca ccttcgccct cgacaccgag cacgagctgg
     1981 tcaatctgcg ggcggtggtc cagggccggg ccacccgggt ccgggccgag gaggtcccgg
     2041 ccggcggctc cgacccgtcg gccgcggcca ccggcaccac caccatctgg gtcggcgacg
     2101 gcgaacggca ggccaccgtc tacgaccggg cccgcctgga ggccggcaac cgcgtccagg
     2161 ggccggcggt ggtcaccgag atggactcga ccacgctggt ccttcccgac cacgtcggcg
     2221 aggtcgaccg ctacggcaac ctgctgctcc gcccggcctc gaacgaaggg agctaggcca
     2281 tggccaccgt catcgagacc aacccgcgcc agttcgcccc ggtcgaggtc gacccggtca
     2341 ccgtcgacat catcgagaac gccctgcgca acacccgcta cgagatggac gcggtgctgt
     2401 tccgcacggc catgtcgccc ggcatccgcg agcagcacga cgagttcccg atcatcgccg
     2461 accgggccgg ccgcatggtc gtcggccagt tcggcagctt catcgacggc ttcctcaaga
     2521 cctaccaggg cgagatctcc gagggcgacg tcttcctcac ctccgacccg tacgcctgcg
     2581 acggcgccat cagccacgcc aacgactggc tggtgctgct gcccatctac cgcgaggggg
     2641 ccctggtcgg ctgggcggcc atgttcggcc acatgaccga cgtcggcggc aaggtcccgg
     2701 gcagcctgcc caccgacgcc acctcgatct acgaggaggg ggtggtcgtc cccccgacca
     2761 agctgtaccg caagggcgag ctggccaccg aggccctgga gctggtcctc aaccaggtcc
     2821 gcatgaagac ctggaaccgc agcgacctca acgccatcgt ggccgcctgc cgcaccgccg
     2881 cccgccgggt gctggagctg tgcgaccgct tcggcaccga gacctacctg tcgaccctgg
     2941 acgccctgct ggaccgcaac taccgggcca tggcccagct catccggacc gccgtcccca
     3001 ccgagcgcct cgagttcacc gactacgtct gcgacgacgg ccgcggctac gggccctaca
     3061 agatccactg ctccatgcag cgggtcgggg acaaggtggt gctcgacttc gacggcaccg
     3121 acccccaggc cgagggctcg atcaacttct acctcaacga gaacatgctg aagatgttct
     3181 tcggcatcta catgatcatg gtcttcgatc cccagatcct ctggaacgac ggcttctacc
     3241 cgctggtcga ggtcaagatc cccgaggggt cgctgctcaa gccccgctac ccggcggcgc
     3301 tgtcgtgccg cacccacgcg ctgggccgga tcttcgacgt catgggcggg ctgctcggcc
     3361 agcgggcgcc cgagttcctg tgcgcggccg gcttctcctc ctcgccccac ctgatgtact
     3421 cgggggtgga ccgcaacggc gagtggttcc agctctacca gatcggcttc ggcggcatcc
     3481 ccggccggcc cttcggcgac gggcccgacg gccattcgct gtggccgtcg ttcaccaacg
     3541 tccccaacga gttcctggag agctacttcc cgctggtcat cgagcgctac cagacggtgg
     3601 ccgactccgg cgggccgggg ttcttccgcg gcggcaacgg catcgacatc a
//
LOCUS       DASSWR010000279         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_604010,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000279 DASSWR010000000
VERSION     DASSWR010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604010"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFO47_04690"
     CDS             complement(<1..222)
                     /locus_tag="VFO47_04690"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET8788024.1"
                     /translation="MTNDTGFVLWLTGLSGAGKSTVAAKLGPALAERGHRVELLDGDD
                     VRTNLCQGLGFSREDRDTNIARIGYVAGKL"
     gene            complement(256..1422)
                     /gene="sat"
                     /locus_tag="VFO47_04695"
     CDS             complement(256..1422)
                     /gene="sat"
                     /locus_tag="VFO47_04695"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET8788025.1"
                     /translation="MTISEQTVTGPRGGLPHGGQLVDRRLDAEEASALASGPLPSLPL
                     TAAEAADLRALATGAYSPLTGFMGAKEHESVTESMRLPDGTLWSIPVCLGIPDGVRVD
                     DGRLALRGANGGLLGVLEVAEVFERDRAAEAELVYGTTDPEHPGVARVLGAPGRAVAG
                     PVQAVVTPLAGPVGERALTPAETRAAFASRGWSTVVGFQTRNPVHRAHEYLMKCALEM
                     VDGLLLHPLVGPTKEGDVPAEVRMRAYEAVLGYFPPERVLLSALVAPMRYAGPREAVL
                     HALVRKNYGCTHFIVGRDHAGVGSYYGTFEAQELIASLPAEDLGITPLRFDNAFYCRR
                     CLASATGKTCPHPDSDHVALSGTQVRAMLAAGQLPPPEFSRAEVAELLIEAYRS"
     gene            complement(1460..2137)
                     /locus_tag="VFO47_04700"
     CDS             complement(1460..2137)
                     /locus_tag="VFO47_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788026.1"
                     /translation="MVMARVLLADSQPLFNEALEAVFSRDDVHQAIGRCSSAEDAFTA
                     VSSLRPDLVLVDAALALEGSPNLVVRMLEACPEARVIVLGDDHDADLLLAAIRAGAAG
                     VVGKTYGASTVLRVAGAVLEGEGAVPRGMLLELARRMVSRDRAPDSPLSRLSPRERQV
                     LALLSRGWDNARIGRDLFISQHTVRTHIQNILEKLGMHSKLEAATFAMQRSMELELTP
                     GGAGTDH"
BASE COUNT          338 a          894 c          972 g          355 t
ORIGIN      
        1 cagcttgccg gcgacatagc cgatgcgggc gatgttggtg tcgcggtcct cgcggctgaa
       61 gccgaggccc tggcagaggt tggtccggac gtcgtcgccg tcgagcagct cgacccggtg
      121 gccgcgctcg gccagggcgg ggccgagctt ggcggccacg gtcgacttgc ccgcaccgga
      181 caggccggtg agccagagca cgaaaccggt gtcgttggtc atgggacgtg gtgccctttc
      241 gttcggcggg ggtggtcagg agcggtaggc ctcgatcagc agctcggcga cctcggcccg
      301 ggagaactcg ggcggtggca gctggccggc ggccagcatc gcccgcacct gggtgccgga
      361 gagggcgacg tggtcgctgt cggggtgcgg gcaggtcttg ccggtggcac tggccaggca
      421 gcgccggcag tagaaggcgt tgtcgaaccg cagcggggtg atgcccaggt cctcggcggg
      481 cagcgaggcg atcagctcct gggcctcgaa ggtgccgtag tagctgccca ccccggcgtg
      541 gtcgcggccg acgatgaagt gggtgcagcc gtagttcttg cggaccaggg cgtgcagcac
      601 ggcctcgcgg ggcccggcgt agcgcatggg cgcgaccagg gccgacagca ggacccgctc
      661 gggcgggaag tagcccagca ccgcctcata ggcccgcatg cggacctcgg cgggcacgtc
      721 gccctccttg gtcggcccga ccagcgggtg cagcagcagc ccgtcgacca tctccagcgc
      781 gcacttcatg aggtactcgt gggcccggtg gaccgggttg cgggtctgga agccgaccac
      841 cgtgctccag ccccgggagg cgaacgcggc ccgggtctcg gccggggtca gggcgcgttc
      901 gcccaccggc ccggccaggg gggtgaccac ggcctggacg gggccggcca cggcccggcc
      961 gggggcgccc agcacccggg ccaccccggg gtgctcgggg tcggtggtgc cgtagaccag
     1021 ctcggcctcg gcggcccggt cgcgctcgaa cacctcggcg acctcgagca cgcccagcag
     1081 cccgccgttc gcgccccgca gggccaggcg gccgtcgtcg acccggaccc cgtcggggat
     1141 gcccaggcac accgggatcg accacagggt gccgtcgggc aggcgcatcg actcggtgac
     1201 cgactcgtgc tccttggccc ccatgaagcc ggtcagcggg ctgtaggcgc cggtggccag
     1261 cgcgcgcagg tcggccgcct cggccgcggt cagcgggagc gatggcagcg ggcccgaggc
     1321 cagcgcgctc gcctcctcgg catccaggcg ccggtcgacg agctgcccgc cgtggggcag
     1381 gccgccgcgg gggccggtca ccgtctgctc gctgatcgtc acagtgcgtg ctccttgtgg
     1441 ttcgttctgg tccgtgcggt cagtggtccg tcccggcgcc gccgggggtc agctcgagct
     1501 ccatcgagcg ctgcatggcg aaggtggccg cctccagctt ggagtgcatg cccagcttct
     1561 cgaggatgtt ctggatgtgg gtgcggacgg tgtgctggct gatgaacagg tcccgcccga
     1621 tccgggcgtt gtcccagccc cggctgagca gggcgagcac ctggcgctcg cgcggggaga
     1681 gccgcgacag cggcgagtcc ggcgcccggt cccgcgagac catgcggcgg gccagctcca
     1741 ggagcatgcc ccgggggacc gcgccctcgc cctccagcac cgccccggcg acccgcagga
     1801 ccgtgctcgc cccatatgtc ttgccgacca ctccggcggc gccggcgcgg atggcggcca
     1861 gcagcaggtc ggcgtcgtgg tcgtcgccga gcacgatcac ccgcgcctcg ggacaggcct
     1921 ccagcatgcg gacgaccagg ttgggcgagc cctcgagggc gagggcggcg tcgaccagca
     1981 ccaggtcggg gcggaggctg gagacggcgg tgaaggcgtc ctcggccgac gagcagcggc
     2041 cgatcgcctg gtggacgtcg tcccgggaga acaccgcctc cagcgcctcg ttgaacaacg
     2101 gctgcgaatc cgcaagcagc acccgtgcca tgaccatcct gtcgtccgag cgagctctgg
     2161 cgagccttgc ggctgcctac tgtggctgat ccacccctcg ctgccctggt acagccgcag
     2221 taagtcgcgc tacgacggcc gacgccgctc gcggatcgct gaaggggggt tcgcgggctg
     2281 tcgggctcct ggccctggat cagtggtcca aagcgtccgg atccgggaag gccgatcatg
     2341 cagtcgtacc atctctagtc acttcttcag atgggcaggc tgtcccctgc gcagtacgtt
     2401 gacggcccac aagtagcgac agggggacta cttcagtggc ttcagttcca cagccggatg
     2461 ctaccgccat gctcgcccac gagctgcggc gccgggtggc ggcggtgcgg gtcgccggcg
     2521 aggcgatcgc cgcgctccgc gaccagggcc gcgatgcca
//
LOCUS       DASSWR010000280         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1112385, whole genome shotgun sequence.
ACCESSION   DASSWR010000280 DASSWR010000000
VERSION     DASSWR010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112385"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFO47_04705"
     CDS             <1..490
                     /locus_tag="VFO47_04705"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8788027.1"
                     /translation="SHFLASVEDTDAGDYDRLMAVNLRGAVLLARAAVPAMRASDGGV
                     IVNVSSEAGLVAVPGQVAYNVSKAGLLMLTRSIAVDHARDGIRAVSVCPGTTRTPLVE
                     RAIHSAPDPQAHERWLASSRPAGRLGVPEEIAAAIVFVASDQASFMTGSEIVVDGGYT
                     AV"
     gene            594..1733
                     /locus_tag="VFO47_04710"
     CDS             594..1733
                     /locus_tag="VFO47_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET8788028.1"
                     /translation="MTLLVGACGRGGDGGTGQGGGRWQEEIVIGWTPPDITGVFKTAT
                     EFFEKSASEATAAGFKVKVESRSPATHTAFADQVAIIDDFVSRKVDVIAISPADTEAI
                     KPAVKRANEANIPVIIVNLLEEQKDIEVASYIGFDNSEAASVSAYAVLDYFGGPGVLG
                     SGEKVDVQPDQYLDLKWWQGVYQNADPASIRGSGSIIEGIAGTFFSQERLDGFHEVVD
                     KYPGIKILGNPIAADWNREKGIKATETFLSRYQPGTELQFIWAASNEMGLGAMLTLER
                     RNVLATTEESPPVKGKVAVFTNDVTPESVARIKEGKLIAETHHGFAEWGWFGTQFAVQ
                     LTCGQQVPKIQDIRPRTAWQGNADQFYPDIALPAIDWNKIKGDCA"
     gene            1736..>2602
                     /locus_tag="VFO47_04715"
     CDS             1736..>2602
                     /locus_tag="VFO47_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788029.1"
                     /translation="MALLELQGARKAYPGVRALQGVDLGVERGEVHALLGENGAGKST
                     LMKVVAGSVTPDAGRMTVDGDPVPLGSLAGAKASGVALVYQDLSLVPQLTVGENVLLG
                     RWPTTRWGTVAWEELHATARRHLGHIGLNVDPRRRIDELGMAERQLVEIAKALSGDVR
                     VLLLDEPTSALSDPEARRLFGIVRDLTSSGVAVVYVSHQLPEVLEIADRISVLRDGQL
                     VGTVRASDVDEARLARMMVGRAVGGGAAAPAPVAGNGAPLAIRASGLARPPRLKPVDL
                     ELRAGEVVAVFGL"
BASE COUNT          394 a          922 c          928 g          358 t
ORIGIN      
        1 cagccacttc ctcgccagcg tcgaggacac cgacgccggc gactacgacc ggctgatggc
       61 cgtcaacctc cggggcgcag tcctgctggc tcgcgccgcc gtcccggcga tgcgggcctc
      121 cgacggcggg gtgatcgtca acgtctccag cgaggccggc ctggtcgcgg tgcccgggca
      181 ggtggcctac aacgtctcca aggccggcct gctgatgctc accaggtcga tcgcggtcga
      241 ccatgcccgt gacggcatcc gcgcggtgtc ggtctgccca ggcaccaccc ggaccccgct
      301 ggtggagcgg gccatccact ccgcccccga ccctcaggcg cacgagcggt ggctggcctc
      361 cagccggccc gcggggcgac tcggcgtccc cgaggagatc gccgccgcca tcgtgttcgt
      421 ggcaagcgac caggcgtcgt tcatgaccgg atcggagatc gtcgtcgacg gcggctacac
      481 cgccgtctag gggaagctcc gggagccgcg tcgccggcgg ccgcccgggg ctcccggagg
      541 gagatgaggt gcagatggct cgctggagca ggaccctcgc gctcttggcc gtcatgacgc
      601 tgctggtggg agcgtgcggt cgcgggggcg acggcggcac tggccagggc ggcggtcgct
      661 ggcaggagga gatcgtgatc ggctggacgc cgccggacat caccggtgtg ttcaagaccg
      721 ccaccgagtt cttcgagaag tccgccagcg aggccaccgc ggccgggttc aaggtcaagg
      781 tggagagccg ctcgccggcc acccacaccg ccttcgccga ccaggtggcc atcatcgacg
      841 atttcgtctc gcgcaaggtc gacgtgatcg ccatctcgcc ggccgacacc gaggcgatca
      901 agccggccgt gaagcgagcc aacgaggcca acatcccggt gatcatcgtc aacctgcttg
      961 aggagcagaa ggacatcgag gtcgccagct acatcggctt cgacaactcc gaggcggcgt
     1021 cggtgtcggc ctatgccgtg ctcgactact tcggcgggcc gggtgtgctc ggcagcggcg
     1081 agaaggtcga cgtccagccc gatcagtact tggacctgaa atggtggcag ggcgtctacc
     1141 agaacgccga ccccgccagc atccgcggct cgggctcgat catcgaaggg atcgccggga
     1201 ccttcttctc ccaggagcgg ctggacggct tccacgaggt ggtcgacaag tacccgggca
     1261 tcaagatcct cggcaacccg atcgccgccg actggaaccg cgagaagggc atcaaggcga
     1321 ccgagacgtt cctgtcccgc taccagccgg gcaccgagct gcagttcatc tgggccgcct
     1381 ccaacgagat ggggctgggg gccatgctca ctctcgagcg ccgcaacgtc ctggccacca
     1441 ccgaggagag cccgccggtg aagggcaagg tcgcggtgtt caccaacgac gtcaccccgg
     1501 agtcggtggc ccgcatcaag gaaggcaagc tgatcgccga gacccaccac ggcttcgccg
     1561 agtggggttg gttcgggacc cagttcgccg tccagctgac ctgcggccag caggtgccca
     1621 agatccagga catccgaccg cgaactgcct ggcagggcaa cgccgaccag ttctaccccg
     1681 acatcgccct gccggcgatc gactggaaca agatcaaggg ggactgcgcc tagccgtggc
     1741 cctgctcgag ctccagggcg cccgcaaggc ctacccgggc gtgcgggcgc tccagggcgt
     1801 ggatctcggc gtggagcgcg gcgaggtcca cgccttgctg ggcgagaacg gcgccggcaa
     1861 gtcgaccctg atgaaggtgg tcgccggctc ggtcaccccc gacgccggcc gcatgaccgt
     1921 cgacggcgac cccgtgccgc tcgggtcgct ggcgggggcc aaggcaagcg gcgtggcgct
     1981 cgtctaccag gacctgagcc tagtgcccca gctcacggtg ggggagaacg tgctgctcgg
     2041 ccggtggccc accacgcgct gggggacggt cgcctgggag gagctgcacg ccacggcgcg
     2101 ccgccacctc ggccacatcg gcctgaacgt cgacccgcgc cggcggatcg acgagctcgg
     2161 catggccgaa cggcagctgg tggagatcgc caaggcgctg tctggcgacg tccgcgtcct
     2221 gctgctggac gagcccacct ccgccctctc ggacccagag gcccgccgcc tgttcggcat
     2281 cgtccgcgac ctcacctcct cgggggtggc ggtggtctac gtctcccacc agctccccga
     2341 ggtgctggag atcgccgacc gcatcagcgt gcttcgcgac gggcagctgg tcgggacggt
     2401 ccgggccagc gacgtcgacg aggcccggct ggcccggatg atggtcggcc gcgccgtcgg
     2461 cggcggcgcc gcagcgccgg cgcccgtagc cggtaacggc gcgccgctgg ccatccgggc
     2521 cagcggcctg gcccggcccc cgcggctcaa gccggtggac ctggagctgc gggcgggcga
     2581 ggtggtggcc gtgttcgggc tg
//
LOCUS       DASSWR010000281         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_548092,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000281 DASSWR010000000
VERSION     DASSWR010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548092"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFO47_04720"
     CDS             <1..243
                     /locus_tag="VFO47_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8788030.1"
                     /translation="RTVLVVTLVSVIGSLLAFDQFYIMTAGQPQNETATSVFYVYLNS
                     FPYLRLGYGAALSMILALTILVFTVVQLLLTRRSHA"
     gene            240..1133
                     /locus_tag="VFO47_04725"
     CDS             240..1133
                     /locus_tag="VFO47_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8788031.1"
                     /translation="MTAIAERRRSRRAVAVGGRQRPRRRGYLLGALCVAICTVMLLPL
                     VVSVLASVKPTAEAAASPPTYLPHGLSLDSYERLWTYQQGLPTYLANSLGTALLTIAF
                     TLALTVPAAYALARFPIPGKEVVFVFLLLALIVPYQALLTPMFLMFARVGLTNTLVGL
                     AILHTAIQLPLSLYVLRNSFSAVPRELEEAAITDGASSWKVLLRVCVPSAKPAIVTVA
                     LFAFIMSWNEFLGALVLMSRGSSFTLPLILAAARTETSLGGTDWGMLQAGITISIIPC
                     VLVYLLLQRYYVSGLMSGAGK"
     gene            1136..>2489
                     /locus_tag="VFO47_04730"
     CDS             1136..>2489
                     /locus_tag="VFO47_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET8788032.1"
                     /translation="MPGPARVTILDGLMAERQRVNRQRTIPHGFDQLERSGTLDNLRL
                     AAGADGRYRALADTSGATFPFLDSDVYKWLEAVGWELGRAADPGLAAAADQAIGLVAA
                     AQRPDGYLNSYVQVVGDGTPHRDLAWGHELYCVGHLIQAAVAWHRELGDDRLLEVAIR
                     AADRIDKELGPGGRDGVDGHPGIEMALVELTRVTGDPRYLALAARMLDLRGKGLLGPG
                     RFGAAYWQDHQPVREAATVAGHAVRQLYLDCGAVDVAVELEDQVLLDAVRRRWQDLVA
                     TRTYLTGGMGSRHRDESFGDPFELPPDRAYAETCAAIASVMLGWRLLLATGEAGYADA
                     IERALYNGVLSGVSLDGTRFFYVNPLQRRTRRAWEPPGDGGRAPWYPCACCPPNLMRL
                     LSSWERYVADADAGGVRLHQHATADVRAEVAGGPVRLSVRTGYPWEGRIAVEITETPE
                     R"
BASE COUNT          305 a          947 c          867 g          370 t
ORIGIN      
        1 cgcacggtgc tggtggtgac gctggtgagc gtcatcgggt cgctgctcgc cttcgaccag
       61 ttctacatca tgaccgccgg gcagccgcag aacgagaccg cgacctcggt cttctacgtc
      121 tacctgaact ccttccccta cctgcggctt ggctacggcg cggccctgtc gatgatcctg
      181 gccctgacca tcctggtctt caccgtcgtg cagctgctgc tcacgcgccg gagccacgcg
      241 tgaccgcgat cgcggagcgg cgccggagcc gccgggccgt ggccgtgggc gggcggcaac
      301 ggccgcgccg gcgcggctac ctgctgggcg ccctgtgcgt ggccatctgc acggtcatgc
      361 tcctgcccct ggtcgtgtcc gtgctggcct cggtgaagcc gacggcggag gcggccgcgt
      421 cgccgccgac ctacctgccg cacggcctca gcctggacag ctacgagcgg ctctggacct
      481 accagcaggg gctccccacc tacctggcca acagcctggg cacggccctg ctgacgatcg
      541 ccttcacgct ggcactgacc gtgcccgcgg cctacgccct ggcccggttc ccgatccccg
      601 gcaaggaggt cgtcttcgtc ttcctcctgc tggcgctgat cgtcccctac caggccctgc
      661 tgacgccgat gttcctgatg ttcgcccggg tcgggctgac caacaccctg gtcggcctgg
      721 ccatcctgca caccgccatc cagctccccc tgagcctgta cgtgctgcgc aacagcttca
      781 gcgccgtccc ccgggagctg gaggaggcgg ccatcaccga cggcgcgtcc tcctggaagg
      841 tgctgctgcg ggtctgcgtc ccctcggcca agccggcgat cgtcaccgtg gccctgttcg
      901 ccttcatcat gtcgtggaac gagttcctcg gcgccctcgt gctgatgagc cgggggtcga
      961 gcttcaccct gccgctgatc ctggccgccg cccggaccga gaccagcctg ggcgggaccg
     1021 actgggggat gctgcaggcc ggcatcacca tctcgatcat cccctgcgtg ctcgtctacc
     1081 tgctgctgca gcggtactac gtgtccggcc tgatgagcgg ggcggggaag tgagcatgcc
     1141 cggtccggcg cgggtcacga tcctggacgg cttgatggcc gagcgccagc gggtcaaccg
     1201 ccagcgcacc atcccgcacg gcttcgacca gctggagcgc tccgggaccc tggacaacct
     1261 gcgcttggcc gccggtgcgg acgggcgcta ccgggcgctg gccgacacct cgggcgccac
     1321 cttccccttc ctggactccg acgtctacaa gtggctggag gcggtgggct gggagctggg
     1381 ccgggccgcc gaccccggcc tggccgccgc cgccgaccag gccatcggcc tggtggcggc
     1441 cgcccagcgc cccgacggct acctcaacag ctacgtccag gtcgtcggcg acgggacgcc
     1501 ccaccgcgac ctcgcctggg gccatgagct ctactgcgtc gggcacctga tccaggcggc
     1561 ggtcgcctgg caccgcgagc tcggcgacga ccggctcctg gaggtcgcga tccgggccgc
     1621 cgaccggatc gacaaggagc tcgggccggg cgggcgcgac ggggtcgacg gccaccccgg
     1681 catcgagatg gcgctggtcg agctgacccg ggtcacgggc gacccacgct acctggccct
     1741 ggcggcccgg atgctcgacc tgcggggcaa ggggctgctc ggcccgggac gcttcggcgc
     1801 cgcctactgg caggaccacc agccggtccg ggaggccgcg accgtcgccg ggcacgccgt
     1861 ccgccagctc tacctcgact gcggcgccgt tgacgtggcc gtcgagctgg aggaccaggt
     1921 cctgctcgac gccgtgcggc gccgctggca ggacctggtc gcgaccagga cgtacctgac
     1981 cggcgggatg ggcagccgcc accgcgacga gtccttcggc gacccgttcg agctgccgcc
     2041 cgaccgggcg tacgccgaga cgtgcgcggc catcgcaagc gtcatgctcg gctggcgcct
     2101 gctgctggcc accggcgagg ccggctacgc cgacgcgatc gagcgggccc tctacaacgg
     2161 tgtcctgtcc ggcgtctccc ttgacggcac ccggttcttc tacgtcaacc cgctgcagcg
     2221 ccggacccgg cgcgcctggg agccgcccgg cgacggcggg cgcgccccct ggtatccctg
     2281 cgcctgctgc ccgcccaacc tgatgcggct gctcagctcc tgggagcggt acgtggccga
     2341 cgccgacgcc ggcggcgtgc ggctgcacca gcacgccacc gcggacgtcc gggcggaggt
     2401 ggccgggggg ccggtgcggc tgtcggtccg taccggctac ccctgggagg gccggatcgc
     2461 ggtcgagatc accgagacgc cggaacggc
//
LOCUS       DASSWR010000282         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_378278,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000282 DASSWR010000000
VERSION     DASSWR010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378278"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1552)
                     /locus_tag="VFO47_04735"
     CDS             complement(128..1552)
                     /locus_tag="VFO47_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8788033.1"
                     /translation="MTSATPPTPTTLGGLRAAGHRYKAVKHELRDNLLARLADGGERF
                     PGIVGFDDTVLPELERALLAGHDIVLLGERGQGKTRLIRSLHHLLDEWTPVVAGCEIN
                     DHPYVPTCVACRRRLDELGDELPVAWRHRSERYGEKLATPDTSVGDLIGDIDPIKVAE
                     GRTLGDPETVHYGLVPRTNRGIFSVNELPDLAERIQVALLNVLEERDIQVRGYQLRLP
                     LDLILIASANPEDYTNRGRIITPLKDRFGAEVRTHYPLRLDDEVTLIRQEAVLAWDEQ
                     PLGRPLVPGHLLEVIARFTRAVRESPQIDARSGVSARFAIAAAETVAASAVRRAAVTG
                     EGLAVARVVDLPAVVPVSLGKVEFDQLEEGRELELLQHLLRRSVAETWRARLAGTDLS
                     GLLRRFDEGATVETGDLVPAGDLLARVGPVPGLAQLLRRLGDDHGAETPGLAAAALEF
                     AMEGLHLAKRLAKEELPGRTVYGG"
     gene            1644..1985
                     /locus_tag="VFO47_04740"
     CDS             1644..1985
                     /locus_tag="VFO47_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8788034.1"
                     /translation="MVRYSSSTLDRTFAALSDPTRREILDRLAEGPATLGELRRPFDM
                     TLPGLMKHVRVLEEAQLVVTEKKGRTRECRLDADPLDDAAQWIGTYRRRWERRLDRLG
                     SYLERRKGASP"
     gene            1982..2440
                     /locus_tag="VFO47_04745"
     CDS             1982..2440
                     /locus_tag="VFO47_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET8788035.1"
                     /translation="MSDDLRLERVYDAAPEIVFDAFTDPDAQKELYADGPEWVVESAC
                     DLRVGGRWTIEFGPPGGPPARETNVFTVVERPRRLGCVSTMTMPDGTSFDTDLEVTFE
                     EEAPGRTRLTIVQRGFPTTGLRDELAGGWPDVLDGLGRVVAARTSGPDRP"
     gene            2437..3206
                     /locus_tag="VFO47_04750"
                     /pseudo
     CDS             2437..3206
                     /locus_tag="VFO47_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          383 a         1152 c         1221 g          450 t
ORIGIN      
        1 ccccagccgg tccagcgccc tggccacgtc gaacggcggc tccagggggt cgggcccgcc
       61 ccgccaggcg ccgtagcggt agctcatccg ccgtggtcgg ccatctgtca ggatctcctc
      121 caggaattca tccgccgtac acggttcggc ccggcagctc ctccttggcc aggcgcttgg
      181 ccaggtggag gccttccatg gcgaactcga gggcggccgc ggccaggccg ggcgtctcgg
      241 ccccgtggtc gtcgccgagg cggcgcagga gctgggccag ccccggcacc ggcccgaccc
      301 gggccagcag gtcgcccgcc gggaccaggt cgccggtctc gacggtggcg ccctcgtcga
      361 agcggcgcag cagccccgac aggtcggtcc cggccagccg ggcccgccag gtctcggcca
      421 ccgaccggcg cagcaggtgc tggagcagct ccagctcccg gccctcctcg agctggtcga
      481 actcgacctt gcccagggag acggggacca ccgccggcag gtcgaccacc cgggccacgg
      541 ccagcccctc accggtcacc gcggcccggc gcacggccga ggcggccacg gtctcggcgg
      601 cggcgatggc gaagcgggcc gagacgccgg agcgggcgtc gatctggggc gactcgcgca
      661 ccgcccgggt gaagcgggcg atgacctcga gcaggtgccc ggggaccagc ggccggccga
      721 ggggctgctc gtcccaggcc agcaccgcct cctggcggat gagggtgacc tcgtcgtcca
      781 ggcgcagcgg gtagtgggtg cggacctcgg ccccgaagcg gtccttgagc ggggtgatga
      841 tgcggccccg gttggtgtag tcctcggggt tggcgctggc gatcaggatc aggtcgaggg
      901 gcagccgcag ctggtagccg cggacctgga tgtcgcgctc ctcgagcacg ttgagcaggg
      961 ccacctggat ccgctcggcc aggtcgggga gctcgttgac gctgaagatg ccccggttgg
     1021 tgcggggcac gagcccgtag tggacggtct cggggtcgcc gagggtgcgg ccctcggcca
     1081 ccttgatggg gtcgatgtcg ccgatcaggt cgccgacgct ggtgtcgggg gtggccagct
     1141 tctcgccgta gcgctcggag cggtgccgcc aggccaccgg cagctcgtcg cccagctcgt
     1201 ccagccgccg ccggcaggcg acgcaggtgg gcacgtacgg gtggtcgttg atctcgcagc
     1261 cggcgaccac cggggtccac tcgtccagca ggtggtgcag ggaccggatg aggcgggtct
     1321 tgccctgccc gcgctcgccc agcagcacga tgtcgtggcc ggcgagcagg gcccgctcca
     1381 gctccgggag gacggtgtcg tcgaagccga cgatcccggg gaagcgctcc cccccgtcgg
     1441 ccagccgggc caggaggttg tcgcggagct cgtgcttgac cgccttgtag cggtgaccgg
     1501 cggcccggag gccgccgagg gtggtggggg tcggcggggt ggcggaggtc atgcgcgccc
     1561 ttctgtcgct tgcggtgcca cgcccatggt ggcctcgcgc gccccattgc gcacccccgg
     1621 ccgggcaagc tatagttaac cagatggtaa ggtattcttc ttcgaccctg gaccggacct
     1681 tcgcggccct gtccgacccg acccgccgcg agatcctcga ccgcctggcc gaggggccgg
     1741 ccaccctcgg cgagctgcgg cggcccttcg acatgaccct cccgggcctg atgaagcacg
     1801 tccgggtcct cgaggaggcc cagctggtgg tgaccgagaa gaagggccgg acccgcgagt
     1861 gccggctcga cgccgatccg ctggacgacg ccgcccagtg gatcgggacc taccggcgcc
     1921 gctgggagcg ccggctggac cggctcggga gctacctgga gcggcggaag ggagcgagcc
     1981 catgagcgac gacctgcggc tggagcgcgt gtacgacgcc gccccggaga tcgtcttcga
     2041 cgccttcacc gacccggacg cgcagaagga gctgtacgcc gacgggcccg agtgggtcgt
     2101 ggagtcggcc tgcgacctgc gggtcggggg ccgctggacg atcgagttcg gcccgccggg
     2161 cgggccgccg gcccgcgaga ccaacgtgtt cacggtggtg gagcggccgc ggcgcctcgg
     2221 ctgcgtgagc accatgacca tgccggacgg gaccagcttc gacaccgacc tggaggtcac
     2281 cttcgaggag gaggcgcccg gccggacgcg gctgaccatc gtccagcgcg gcttcccgac
     2341 caccgggctc cgcgacgagc tcgccggcgg ctggcccgac gtcctggacg ggctcggccg
     2401 ggtcgtcgcc gccaggacgt ccgggccgga ccgcccatga gcacggtcgc cacccgccgg
     2461 gccggtcgcc gggagtgggc cgggctggcc gtgatcgccc tgccctgcct gctcgccacc
     2521 atggacctgg aggtgctgaa cctggccgtc ccggccctga ccgcggacct gcagcccagc
     2581 agcgcccagc tgctgtggat catcgacatc tacgggttca tggtcgccgg gtcgctgatc
     2641 accatgggaa ccctcgggga ccgcatcggc cgccgccggc tcctgctgat cggcgcggcc
     2701 gccttcgggg tcgcctccgt gctggccgcc ttctccacca gcgccgagat gctgatcgcg
     2761 acccgggcgc tgctgggcgt ggccggggcg accctggccc cctcgaccct gtcgctgatc
     2821 cgcaacatgt tcccgacccg gggcagcgca cggtcgccgt ggccgtgtgg gcgaccagct
     2881 tctcggtcgg ggccgccatc gggccgctgc tgggcggggt gctcctggag tggttctggt
     2941 gggggtcggt gttcctgctg gccgtgcccg tgatggtgct gctgctggtg gttggcccgg
     3001 tgctgcgacc cgagtacaag gacccggacg cgggccggct ggatctccgc agcgcggcca
     3061 tgtcgctggc cgcggtgctg ctggtgatct acgggctcaa gcagctcgcc ctggacggcc
     3121 tcgggcccga gccgtcgctg tcggtggtgg tcgggctggc cgtcggctgg ctgttcgtcc
     3181 gccgccagcg caccctggcc gacccg
//
LOCUS       DASSWR010000283         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1282532, whole genome shotgun sequence.
ACCESSION   DASSWR010000283 DASSWR010000000
VERSION     DASSWR010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282532"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFO47_04755"
     CDS             complement(<1..438)
                     /locus_tag="VFO47_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SARP family transcriptional regulator"
                     /protein_id="HET8788036.1"
                     /translation="MVRIRLLGPPAIERDGAPAVPRGRKTWALLAYLLLAEQPPSRRH
                     LAELLFGEADDPLGALRWSLAELRRTLGRPGLFAGDPVATGLGEDVTVDLQDVTGEPA
                     DPGRLLDLGGELLDGLSVAGSPMFESWLVVERHRLTSMAEARLR"
     gene            complement(455..1285)
                     /locus_tag="VFO47_04760"
     CDS             complement(455..1285)
                     /locus_tag="VFO47_04760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8788037.1"
                     /translation="MSTTTTQTAQPDLAAVKARQQKMWASGNYAAVAARIVPMAEHLA
                     EAAGLRPGDRVLDVATGSGNAALAAARYGCEVTGVDYVPELLERGRARAAAEGLEITF
                     AEGDAEDLAFPDASFDAVLSVLGVMFAPDQERAAAELVRVCRPGGTIGLVNWTPAGFI
                     GQLLKTVSKHVPPPAGVRPPPQWGTEERLRELLGDAVSRLEVTKRSHVWRFRSPEDFA
                     GFFKDQYGPTRKAFEALDEAGQRRLWDDLTALAREHDTERGLTVAMPAEYVEVIATRA
                     "
     gene            1423..1818
                     /locus_tag="VFO47_04765"
     CDS             1423..1818
                     /locus_tag="VFO47_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788038.1"
                     /translation="MRRTHPPPPAPARAPVAPLPGPLQPLWALALAIAESAAEIAATR
                     PIPSWRAGAYLYTAADQAWGADGGGGAAGWRTDRGGSGGEFRFAVLLAEAATARFVAG
                     DGYLSNRQLAADLRSGAAWLATGALLPSR"
     gene            complement(1815..>2087)
                     /locus_tag="VFO47_04770"
     CDS             complement(1815..>2087)
                     /locus_tag="VFO47_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788039.1"
                     /translation="EMDADLPEWSAADVRNLAAPTMLIIGDSDIVQPEHTVELFRLRG
                     GGVIGDLAGLPPARLAVLPGTTHVTLVQRAEWLSSMVGEFLDAEVG"
BASE COUNT          256 a          772 c          804 g          255 t
ORIGIN      
        1 ccgcagccgc gcctcggcca tggaggtgag ccggtggcgc tccaccacca gccacgactc
       61 gaacatcggg ctgccggcga cgctgaggcc gtcgagcagc tcgccgccca ggtcgagcag
      121 gcgcccgggg tcggccggct cgccggtgac gtcctggagg tcgaccgtca cgtcctcgcc
      181 gaggccggtc gcgaccgggt cgccggcgaa cagcccgggc cgccccaggg tccgccgcag
      241 ctcggccagg gaccagcgca gcgcacccag cgggtcgtcg gcctcgccga acagcagctc
      301 ggccaggtgg cggcggctgg gcggctgctc ggccagcagc aggtaggcca gcagggccca
      361 cgtcttgcgg ccgcgcggca ccgccggcgc accatctcgt tcgatggccg gcgggccgag
      421 caggcggatc ctcaccacgg caggctccct ggaactacgc gcgggtcgcg atcacctcca
      481 cgtactccgc gggcatcgcc accgtcaatc cccgctcggt gtcgtgctcg cgggccaggg
      541 cggtcaggtc gtcccagagc cgccgctggc cggcctcgtc gagggcctcg aaggccttgc
      601 gggtcgggcc gtactggtcc ttgaagaacc cggcgaagtc ctcgggggag cggaagcgcc
      661 acacatggct gcgcttggtg acctccaggc gcgacacggc gtcgccgagc agctcgcgca
      721 ggcgctcctc ggtcccccac tggggaggcg gccggacccc ggccgggggc ggcacgtgct
      781 tggagacggt cttgaggagc tggccgatga agcccgccgg ggtccagttg accaggccga
      841 tggtgccgcc ggggcggcag acgcggacca gctcggccgc ggcccgctcc tggtcgggcg
      901 cgaacatgac gccaaggacg gacaggacgg cgtcgaagga ggcgtcggga aaggccaggt
      961 cctcggcgtc gccctcggcg aaggtgatct ccagcccttc ggccgccgcc cgggcccggc
     1021 cgcgctccag cagctcgggg acgtagtcga cgccggtgac ctcgcagccg tagcgggcgg
     1081 cggccagggc ggcgttgccg ctgccggtgg ccacgtcaag gacgcggtcg ccgggccgca
     1141 ggccggccgc ctcggccagg tgctcggcca tgggcacgat ccgggcggcc acggcggcgt
     1201 agttgccgct ggcccacatc ttctgctggc gcgccttgac ggcggccagg tccggctggg
     1261 cggtctgggt ggtggtggtg ctcatctgct cctccttggg catgggggtc ggtggaaagc
     1321 atccggcacc ggcgtgtgaa cgggcgtgtg acgtgaggat ccggttagcg ggcggtccca
     1381 tgctgcggag atccgcacgc cagctggagt gaggacggtc ccatgcgacg aacccacccg
     1441 cccccgccgg cccccgcccg ggcgccggtc gcccccctcc ccggcccgct gcagccgctg
     1501 tgggcgctgg ccctggccat cgccgagtcg gcggccgaga tcgccgccac ccggcccatc
     1561 cccagctggc gggccggcgc ctacctgtac accgccgccg accaggcctg gggcgccgac
     1621 ggcggtggtg gcgcggccgg ctggcggacc gaccgcggtg ggtcgggcgg cgagttccgc
     1681 ttcgcggtcc tgctggccga ggcggccacc gcccgcttcg tggccggcga cggctacctg
     1741 agcaaccgcc agctggccgc cgacctccgc tccggcgccg cctggctggc caccggggcg
     1801 ctgctgccga gccgctagcc gacctcggcg tcgaggaact cgccgaccat cgaggagagc
     1861 cactcggccc gctggaccag ggtgacgtgg gtggtgccag ggaggacggc caggcgggcg
     1921 ggcggcagcc cggccaggtc gccgatcaca ccgccgccgc gcaggcggaa gagctccacg
     1981 gtgtgctcgg gctggacgat gtcggagtca ccgatgatca gcatggtcgg ggcggcgagg
     2041 ttccggacgt cggcggccga ccactcgggc aggtccgcgt ccatctc
//
LOCUS       DASSWR010000284         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_180740,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000284 DASSWR010000000
VERSION     DASSWR010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180740"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1026
                     /locus_tag="VFO47_04775"
     CDS             103..1026
                     /locus_tag="VFO47_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8788040.1"
                     /translation="MTPPGPGVHRPLTRRQALAAGMVPLLSAVAGCDAAGTGKAPIEP
                     PAPTTVPAPPKGPVRAGLVAFGDFGGGRAQAAVARAMERWAARHRIDALVTTGDNVYE
                     RGEPELFAAQLDEPYRELRRTRPLWATLGNHDIAGGHGATQLRHLGLPNLPYARTLPG
                     LRLLLLDANRVDDAQTAWLDDQLAAPGPEPRVVVFHHAAWSCSRHDSNERVRRAWVPV
                     LERHRVPLVLNGHDHNYQRFVSARGVTYVVTGGGGRDLYPLDGCAAGTPERVAAHVRH
                     HFTAVEVRDGSLAVTVVADDDTVLDRAVIGR"
     gene            complement(1013..1426)
                     /locus_tag="VFO47_04780"
     CDS             complement(1013..1426)
                     /locus_tag="VFO47_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788041.1"
                     /translation="MRTDLAKAALIRRRFARRPEEVAAARRFVRGGLTRGSEAAWETA
                     QLLVSETVTSSLDRTASDTGSGTLEVGYAVVGRRLRVEVSDDGGPARLRRRIGAVRSA
                     GGRGLELVRALASRWGVRESSAGRTIWFEVDLSGR"
     gene            complement(1496..2305)
                     /locus_tag="VFO47_04785"
     CDS             complement(1496..2305)
                     /locus_tag="VFO47_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788042.1"
                     /translation="MRVGMVLVVVAAVAAATVGLLWVFQRRLIYLPSGNVPPAASVLA
                     GAEDVTFETADGLRLGGWFVPGAGGRGPAVLVCNGNGGNRAMRAALAAALARMGLAVL
                     LFDYRGYGGNPGSPTEEGLAADARAAAGYLAARPEVDPARLVYFGESLGAAVALRLAT
                     ERPPAALVLRSPFASLAEVGRVHYPVLPVSWLLRDRFDSAALAGRLDAPLLVVAGGRD
                     GIVPVGHSRRLFAAAPQPKRLVVLDGADHNDPELLAGPRLLEELRAFLAEV"
BASE COUNT          313 a         1005 c          891 g          244 t
ORIGIN      
        1 cccccggacc cgcctgcgcg gcccggccaa ggtggtgatg gtcctcggca accacgatga
       61 ccacctccac gtccgcctcc atcccgcgac ccgcagccgc cggtgacccc gcccggccca
      121 ggcgtccacc ggcccctgac ccgccgccag gcgctggcgg ccgggatggt cccgctgctg
      181 tcggcggtgg ccggctgcga cgctgccggg accggcaagg ccccgatcga gccgccggcc
      241 ccgaccaccg tccccgcgcc gccgaagggg ccggtccggg ccgggctggt cgcctttggc
      301 gacttcggcg ggggacgggc ccaggcggcg gtggccaggg cgatggagcg ctgggcggcc
      361 cggcaccgga tcgacgcgct ggtcaccacg ggggacaacg tctacgagcg gggcgagccc
      421 gagctgttcg ccgcccagct ggacgagccc taccgggagc tgcgccgcac ccggccgctg
      481 tgggcgacgc tcggcaacca cgacatcgcc ggcggccacg gcgccaccca actgcgccac
      541 ctcggcctgc ccaacctgcc ctacgcccgc acgctgcccg gcctgcggct gctgctcctg
      601 gacgccaacc gggtcgacga cgcccagacg gcctggctgg acgaccagct ggccgcgcca
      661 gggccggagc cgcgggtggt cgtgttccac cacgccgcct ggtcgtgcag ccgccacgac
      721 tccaacgagc gggtgcgccg cgcctgggtg cccgtgctgg agcgccaccg ggtaccgctg
      781 gtgctcaacg gccacgacca caactaccag cgtttcgtct ccgcccgcgg ggtcacctac
      841 gtggtcaccg gcgggggcgg gcgggacctc tacccgctgg acggctgcgc cgccggcacc
      901 cccgaacggg tcgccgccca cgtccgccac cacttcaccg ccgtggaggt ccgcgacggg
      961 tcgctggccg tgacggtggt cgccgacgac gacaccgtcc tcgaccgggc cgtcatcggc
     1021 cgctgaggtc gacctcgaac cagatggtcc gcccggccga gctctcgcgc accccccagc
     1081 ggctggccag ggcgcggacc agctccagcc cccggccgcc ggccgagcgg acggcgccga
     1141 tgcgccgccg cagccgcgcc ggcccgccgt cgtcggacac ctcgacccgg agccggcggc
     1201 cgaccaccgc gtagccgacc tccagggtgc cgctgccggt gtccgaggcg gtgcggtcga
     1261 gcgagctggt caccgtctcg gagaccagca gctgggcggt ctcccaggcc gcctcggagc
     1321 cgcgggtcag gccgccccgg acgaaccgcc gggcggcggc cacctcctcg ggacggcggg
     1381 cgaagcgtct gcggatcagt gcagccttgg ccaggtccgt tcgcacgggc gctcctcggc
     1441 tcgacggcag ggtgggggga ccgtctcgcc cggctccttc ccaatcgtgg cggggctaaa
     1501 cctcggccag gaacgcccgc agctcctcca gcagccgggg gccggcgagc agctcggggt
     1561 cgttgtggtc ggcgccatcc aggaccacca gccgcttggg ctggggcgcg gccgcgaaca
     1621 ggcgccggct gtgcccgacg gggacgatgc cgtcgcggcc gccggccacc accagcaggg
     1681 gcgcgtccag ccgcccggcc agcgccgccg agtcgaaccg gtcccgcagc agccacgaca
     1741 ccggcagcac gggatagtgc acccgcccga cctcggccag ggacgcgaac ggcgaccgca
     1801 gcaccagcgc cgccggcggc cgctcggtgg ccagccgcag cgccaccgcc gcccccagcg
     1861 actccccgaa gtagaccagc cgggccgggt cgacctcggg ccgggcggcc aggtacccgg
     1921 ccgccgcccg ggcgtcggcg gccagcccct cctcggtcgg gctgcccggg ttcccgccgt
     1981 acccgcggta gtcgaacagc agcaccgcca gccccatccg ggccagcgcg gcggccagcg
     2041 ccgcccgcat ggcccggttc cccccgttgc cgttgcacac cagcaccgcc ggtccccgcc
     2101 ccccagcgcc ggggacgaac cagccgccga ggcggaggcc gtcggcggtc tcgaaggtga
     2161 cgtcctcggc gccggccagg accgaggcgg ccgggggcac gttccccgac gggaggtaga
     2221 tcaggcggcg ctggaacacc cagagcagcc ccaccgtggc cgccgcgacc gcggccacga
     2281 ccaccagaac catcccgacg cgcatccctg agccgtacca tgatcgtcgc tcgggcgcac
     2341 catcggctga accagcggcg gggagggagc gagcatggag gtcggggtgg ggctgcccac
     2401 gaccacgccg ggcgccgacg ggcgcgccgt cctcgagtgg gccaggcggg ccg
//
LOCUS       DASSWR010000285         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_9248,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000285 DASSWR010000000
VERSION     DASSWR010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9248"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFO47_04790"
     CDS             complement(<1..229)
                     /locus_tag="VFO47_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788043.1"
                     /translation="MSAAAPDAGRAPGLDRTGEPQTLAAPAGLVSDVLAITGRAIRGI
                     LREPELFIFALIIPVFFFVVQIGALGDVAEQQ"
     gene            complement(226..1260)
                     /locus_tag="VFO47_04795"
     CDS             complement(226..1260)
                     /locus_tag="VFO47_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788044.1"
                     /translation="MSAIQATDLVRRFGEVEAVRGVSLEVAEGEIYGFLGPNGAGKST
                     IVRILTTLLQPTSGSGAVLGHDVTAEPTTVRRLIGVALQEAGLDPKQTGRELLTLQGK
                     LYGLKGKVLGDQVTEVTELVGLTDAIDRRLQTFSGGMKRRLDLASSLLHRPRVLFLDE
                     PTTGLDPLSRALIWDRIRELRERFGATIFLTTQYLEEADELADRVAIIDHGRIVRQGT
                     PAALKAEIGADVIDLAVEDRESGAARAAITELQASGELPAGELRGTQAGYTLFVPDGP
                     QNIAVLIRGLDKAGVRFGAVSVSRPTLDDVFLAATGGRMAAADQDGQGQGEDGQGRDQ
                     EPAATGRRAS"
     gene            complement(1317..2117)
                     /locus_tag="VFO47_04800"
     CDS             complement(1317..2117)
                     /locus_tag="VFO47_04800"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8788045.1"
                     /translation="MRLVSFEGGFGRVEDDAVVPMGPSLVDWLAFGTQGVATGKPRPL
                     AEVRLLAPVPRPGKVICVGLNYRDHARETRQELPTEPVLFPKYHNSVVGPGADVLVPP
                     EAEKIDYEAELAVVVGRRTSRVAEADALDHVAGYACANDVSSRSLQFRSAQWLLGKAI
                     DTFLPLGPFLVTADEVADPQALGIRCRVNDELRQDSNTGEMVFGVAELVSFISRTITL
                     EPGDLVVTGTPSGVGMAADPPRFLRPGDRMRVEIDGLVELDNTVRPRP"
     gene            complement(2145..>3094)
                     /locus_tag="VFO47_04805"
     CDS             complement(2145..>3094)
                     /locus_tag="VFO47_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8788046.1"
                     /translation="QLGLAAVWGSEHHAVEDGHLSQQLPFLAAVAARTSRIRLGTGVL
                     LLPLYRPREVAEQAGVVDLLAAGRLVLGLGGGWVEREFDAFGVDRSRRGRLLEEKLGI
                     LRRALAEGVAGDGPGGSDLPVGPRSPQAGGPPLYLGGTAPKALDRVARLGDGWFALAH
                     YRWQKTAEGWPVLAEALERAGRPVDGFPVVVGVHLRVSDDPERTWATELAPAVAYQLD
                     RYGDWTADRDQPRPDPIDPGRLKRSAVLCDTPEGIVAVLTQFQEQLPFTHVSLWSRPP
                     GTSHEAACENLERVAREVAPAFAGPEPPWRAPAATPAAG"
BASE COUNT          392 a         1098 c         1204 g          400 t
ORIGIN      
        1 gctgctgctc ggccacgtcg ccgagggcgc cgatctgcac cacgaagaag aacacgggga
       61 tgatcagcgc gaagatgaac agctcgggct cgcgcaggat gccgcggatg gcccggccgg
      121 tgatggccag gacgtcggag accagcccgg ccggcgcggc cagggtctgc ggctcgccgg
      181 tccggtccag ccctggggcg cgtccggcgt cgggggcggc ggcgctcacg atgccctcct
      241 tccggtggcg gcgggctcct ggtcccggcc ctgaccgtcc tcgccctggc cctggccgtc
      301 ctggtcggcg gcggccatgc gcccgccggt ggcggccagg aacacgtcgt ccagggtcgg
      361 ccgggacacg ctgacggctc cgaagcggac gccggccttg tccaggccgc ggatgagcac
      421 ggcgatgttc tgcggcccgt cgggcacgaa cagggtgtag ccggcctggg tgccgcgcag
      481 ctcgccggct ggcagctcgc cgctggcctg gagctcggtg atggcggccc gggcggcgcc
      541 ggactcgcgg tcctcgacgg ccaggtcgat cacgtcggcg ccgatctcgg ccttgagcgc
      601 cgcgggggtg ccctggcgga cgatgcggcc gtggtcgatg atcgccaccc ggtcggccag
      661 ctcgtcggcc tcctccaggt actgggtggt gaggaagatg gtggccccga accgctcgcg
      721 gagctcgcgg atgcggtccc agatcagcgc ccgggacagc gggtcgagcc cggtggtggg
      781 ctcgtccagg aacagcaccc tgggccggtg cagcagggac gaggccaggt cgaggcggcg
      841 cttcatgccc ccggagaagg tctggaggcg ccggtcgatg gcgtcggtca ggccgaccag
      901 ctcggtcacc tcggtgacct ggtcacccag gaccttgccc ttgagcccgt agagcttgcc
      961 ctggagggtg agcagctcgc ggccggtctg cttggggtct aggccggcct cctggagggc
     1021 gaccccgatg agtcgccgca ccgtggtcgg ctccgccgtg acgtcgtgcc ccagcacggc
     1081 gcccgacccg ctggtcggct ggagcagggt ggtgaggatg cggacgatgg tcgacttgcc
     1141 ggccccgttg gggcccagga agccgtagat ctcgccctcg gcgacctcga ggctgacgcc
     1201 gcgaacggcc tcgacctcgc cgaaccgacg gaccaggtct gttgcttgga ttgcggacat
     1261 gcagccgagt gtaggggact cccccgacat tgccacggag ttatcgactg cgctggtcag
     1321 ggccttggtc ggaccgtgtt gtcgagctcg acgaggccgt cgatctcgac ccgcatgcgg
     1381 tcacccggac gcaggaaccg gggtgggtcg gcggccatgc cgacccccga cggggtgccg
     1441 gtgacgacca ggtcgcccgg ctccagggtg atggtccggc tgatgaagct gaccagctcg
     1501 gccaccccga acaccatctc gcccgtgttc gagtcctggc gcagctcgtc gttgacccgg
     1561 cagcggatgc cgagggcctg cgggtcggcc acctcgtcgg ccgtgaccag gaacggcccc
     1621 aggggcagga aggtgtcgat cgccttgccc agcagccact gggcggagcg gaactggagc
     1681 gaccgcgagg agacgtcgtt ggcgcaggcg tagccggcga cgtggtcgag ggcgtcggcc
     1741 tcggccaccc ggctggtccg gcggcccacg accacggcca gctcggcctc gtagtcgatc
     1801 ttctccgcct ccgggggcac gagcacgtcg gcgccgggac cgacgaccga gttgtggtac
     1861 ttgggaaaca ggaccggctc cgtggggagc tcctggcggg tctcgcgggc gtggtcgcgg
     1921 tagttgaggc cgacgcagat gaccttgccc ggccggggca cgggggccag cagccgcacc
     1981 tcggccaggg ggcgcggctt gccggtggcc acgccctggg tgccgaaggc gagccagtcg
     2041 accagcgacg ggcccatggg caccaccgcg tcgtcctcga cgcggccgaa tcccccctcg
     2101 aacgagacca gccgcacggg tcctcctcgg caacggggcc ggcgtcagcc ggccgctggg
     2161 gtggccgccg gagcccgcca cgggggctcg gggccggcga aggcgggggc gacctctctg
     2221 gccacccgct ccaggttctc gcaggcggcc tcgtggctgg tgcccggcgg gcggctccac
     2281 aggctcacgt gggtgaaggg gagctgctcc tggaactggg tcaggacggc cacgatcccc
     2341 tccggggtgt cgcagagcac ggccgagcgc ttcaggcggc cggggtcgat cgggtcgggg
     2401 cggggctggt cgcggtcggc ggtccagtcg ccgtagcggt cgagctggta ggcgacggcc
     2461 ggggccagct cggtggccca ggtgcgctcg gggtcgtcgc tgacccgcag gtggacgccg
     2521 accacgaccg ggaagccgtc gaccggccgg ccggcccgct ccagggcctc ggccagcacc
     2581 ggccagccct cggccgtctt ctgccagcgg tagtgggcca gggcgaacca gccgtcgccc
     2641 agcctggcca cccggtccag cgccttgggg gcggtgccgc ccaggtacag gggcggaccg
     2701 ccggcctggg gcgagcgggg gccgacgggc aggtcgctgc cgccggggcc gtcgccggcc
     2761 accccctcgg ccagggcgcg gcgcaggatg cccagcttct cctccaggag ccggccccgg
     2821 cgggaccggt cgaccccgaa ggcgtcgaac tcgcgctcca cccagccgcc gcccaagccc
     2881 agcaccagcc ggccggcggc cagcaggtcg accacgccgg cctgctcggc cacctcccgg
     2941 ggccggtaca ggggcagcag cagcacccct gtgcccagcc ggatgcgcga ggtccgggcg
     3001 gccacggcgg ccaggaaggg gagctgctgg gacaggtggc cgtcctcgac ggcgtggtgc
     3061 tcgctccccc agacggcggc taggccgagc tgct
//
LOCUS       DASSWR010000286         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_435001,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000286 DASSWR010000000
VERSION     DASSWR010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435001"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="ccsA"
                     /locus_tag="VFO47_04810"
     CDS             complement(<1..663)
                     /gene="ccsA"
                     /locus_tag="VFO47_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET8788047.1"
                     /translation="MKRSEQMMARLTGRRGERALAGAALAGLAASAGLSLLVAPPDAV
                     QGEVQRLMYVHVPAAWLAYLSFTSVFAASVAYLVTSRTRWDRLAAASAEVGVLFCALT
                     IVLGALWGRPVWGTWWTWDPRLTTTAVLLLIYLGYLALRQMADSPGRRARWSAVLGVV
                     GFVDVPIVHMSVVWWRSLHQAPTVLRPGAPTIAGSMLAALLVAVAAFTLAWAYLITVR
                     LRV"
     gene            complement(690..2015)
                     /locus_tag="VFO47_04815"
     CDS             complement(690..2015)
                     /locus_tag="VFO47_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788048.1"
                     /translation="MALGRLLLLLMAAGVLFELSGSSSAREQPQAARGDQPVQTQATR
                     LVDQGRQIFRFDTFGDEQFWGDSLKLHRAVAGAANGGVGPGLSPRAALGVGLKVDVDA
                     LPPELQQALQRGEVDLDDPAVTLALLKLGAVVGVTGFFDSSGGLRSMGIQCALCHSTV
                     DDSFAPGIGHRLDGYAARDLNVGAIVSLAPDLRPVARLLGTDVATVKKVLAAWGPGKF
                     DAELFLDGKGFRPDGKTAATLIPPAYGLAGVNLHTYTGWGSVPYWNAFVANLEMHGQG
                     VFYDPRLDNAKQFPVAARNRSGHVTNSPDLITPKLAALHFYQLALAAPKPPDGSFDPA
                     AAARGARLFRGKAECATCHVPPLFTEPGWNLHTPAEIGIDRFQADRSPTHRYRTTPLK
                     GLWAHQKGGFYHDGRFATLGQVVDHYDRFFELGLTPREKQDLAQYLKSL"
     gene            complement(2207..>2449)
                     /locus_tag="VFO47_04820"
     CDS             complement(2207..>2449)
                     /locus_tag="VFO47_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET8788049.1"
                     /translation="LAAVGSLFGVLAVVPRAREAALPLLVMPLATPVLVAGVKATELA
                     VAGRGGEAGSWLGLLVAFDLVFLAAGTLVFEYLVEE"
BASE COUNT          383 a          872 c          900 g          294 t
ORIGIN      
        1 gacccgcagc cgcacggtga tcaggtaggc ccaggccagg gtgaaggcgg ccacggcgac
       61 caggagcgcg gcgagcatgc tgccggcgat cgtcggggcg ccaggccgta gcacggtggg
      121 cgcctggtgc agcgagcgcc accacaccac cgacatgtgg acgatcggca cgtccacgaa
      181 cccgaccacc cccagcaccg ccgaccagcg ggcccggcgg ccggggctgt cggccatctg
      241 ccgcagggcg aggtagccca ggtagatcag cagcaggacc gcggtggtgg tcaggcgcgg
      301 gtcccaggtc caccaggtgc cccacaccgg cctgccccac aacgccccca gcacgatcgt
      361 gagggcacag aacagcaccc cgacctcggc cgaggcggcg gccaggcggt cccagcgcgt
      421 ccgcgacgtc accaggtagg cgacgctggc cgcgaacaca gaagtgaacg acaggtaggc
      481 cagccaggcg gccgggacgt gcacgtacat cagccgctgc acctcgccct ggacggcgtc
      541 gggcggcgcc accagcagcg acagcccggc gctggcggcg agcccggcca gggccgcgcc
      601 cgccaaggcc cgctccccgc gccggccggt gagcctggcc atcatctgtt cgctgcgctt
      661 catggcgacc tccgggcagg caggcctgcc tacagcgact tcaggtactg ggccaggtcc
      721 tgcttctccc tgggggtgag gccgagctcg aagaagcggt cgtagtggtc gaccacctgc
      781 ccgagggtgg cgaaacgacc gtcgtggtag aagccgccct tctggtgcgc ccacagcccc
      841 ttcaacggcg tcgtgcgata ccggtgggtg ggcgagcggt cggcctggaa gcggtcgatg
      901 ccgatctcgg cgggggtgtg caggttccag ccgggctcgg tgaacaacgg cggcacgtgg
      961 caggtggcgc actccgcctt gccgcggaac agccttgccc cgcgggcggc ggcggccggg
     1021 tcgaaggagc catcgggtgg cttcggcgca gccagggcga gctggtagaa gtggagcgcg
     1081 gccagcttgg gcgtgatcag gtcgggtgag ttggtgacgt gcccggaccg gttcctggcc
     1141 gccaccggga actgcttggc gttgtcgagg cgcgggtcgt agaagacgcc ctgaccgtgc
     1201 atctcgagat ttgccacgaa ggcgttccag tacgggaccg agccccagcc ggtgtaggtg
     1261 tgcaggttca ccccggccag gccgtaggcg ggtgggatca gggtggcggc ggtcttgccg
     1321 tcgggccgga accccttgcc gtccaggaac agctcggcgt cgaacttgcc cggcccccac
     1381 gcggccagca ccttcttgac ggtggccacg tcggtgccga ggaggcgggc gaccggccgc
     1441 aggtcgggcg ccagggagac gatcgcgccg acattcaggt cgcgggcggc gtagccgtcc
     1501 agccggtgac cgatgcccgg cgcgaacgag tcgtcgacgg tggagtggca cagggcgcac
     1561 tggatgccca tcgagcgcag gccgccgctc gagtcgaaga acccggtgac gccgaccacc
     1621 gcccccagct tgagcagcgc cagcgtgacc gcagggtcgt ccaggtcgac ctcgccccgc
     1681 tggagggcct gctgcagctc gggaggcagg gcgtcgacgt cgaccttcag cccgaccccg
     1741 agcgccgccc ttgggctcag gccgggcccg acgccgccgt tcgcggcccc ggcgaccgcc
     1801 cgatggagct tcaggctgtc gccccagaac tgctcgtcgc cgaaggtgtc gaagcggaag
     1861 atctggcggc cctggtcgac gagccgcgtc gcctgcgtct gcaccggttg atcgccgcgg
     1921 gccgcctgcg gttgctcccg ggccgatgac gaccccgaga gctcgaacag gacgcccgcc
     1981 gccatgagca gcaacagcag gcgccccagc gccacggccc agcgccgcgg gcgcccacgt
     2041 ggcgcgtccg ccggctcggg ggacggcgca ggctctggcg ccgcgccggt ggggtcagtg
     2101 gtcccggcca tgggtcacca cctcgggatc ccgaagctcc taataccaat gaaaactagt
     2161 attaggggac tctggcaccg cctttggcgg cgtgtcaagg gtccggctac tcctcgacga
     2221 ggtattcgaa gaccaacgtg cccgcagcca ggaacaccag gtcgaacgcg accagcaagc
     2281 cgagccagga gccagcctcg ccgccgcgcc cggcaacggc cagctcggtc gccttgaccc
     2341 ccgcgaccag cacgggcgtg gccagcggca tgaccagcag gggcagcgcc gcctcgcggg
     2401 cccgcgggac caccgcgagc acgccgaaga ggctgccgac ggcggcaag
//
LOCUS       DASSWR010000287         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_802205,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000287 DASSWR010000000
VERSION     DASSWR010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802205"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFO47_04825"
     CDS             <1..706
                     /locus_tag="VFO47_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET8788050.1"
                     /translation="AVAAIAIAVGVAYLIRTPLFGLSDVRIEGTRAVTKAEVVAASQV
                     RLGEPYLGLDLAAIRARVAALPRVAAVRVARDYPSSLRITVSERPPVASVSSGSVFWL
                     VAADGTVLDAASRRPAELPYVAAVPLPDGVRPGSRLPPGNELANALTALGGMAPQLKR
                     QVTGVNARSLDSLEFTLQDGSRVLYGLAVDQPAKDAAVLLVRRTLKREGREVQRIDVR
                     NPSAPTVRATPANNSG"
     gene            874..2076
                     /gene="ftsZ"
                     /locus_tag="VFO47_04830"
     CDS             874..2076
                     /gene="ftsZ"
                     /locus_tag="VFO47_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET8788051.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAVNRMIEASLKGVEFIAVNTDA
                     QALLASDADVKLDVGRELTRGLGAGSNPDVGRQAAEDHAEELEEVLKGADMVFVTAGE
                     GGGTGTGGAPVVARIAKAQGALTIGVVTRPFAFEGKRRSAQADKGIESLKQEVDTLII
                     IPNDRLLQVAERTTSMLDAFKLADQVLLQGVQGITDLITIPGLINLDFADVRATMREA
                     GTALMGIGQARGEDRAVAAAKQAISSPLLEASVDGARGVLLNISGGSDLGLFEVNEAA
                     EVIASAAHPDANIIFGAVIDDALGDEVRVTVIAAGFDRTAEQTAADGGRPQAAGSAED
                     ESVWPTPGMRRANDSRVSRPAPPTSSPGPAERREPERREPERREPDRRPRYDGDDDEL
                     DIPSFLKR"
     gene            2096..>2554
                     /locus_tag="VFO47_04835"
     CDS             2096..>2554
                     /locus_tag="VFO47_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HET8788052.1"
                     /translation="MLAWRPGPPTAGAPASVWPPVLVAEDLLEAGVVAAFTGRAGGGS
                     TPPYDTLNLGLRVGDDLRRVLANRRRVATVLGLAGRPWALARQVHGAEILRVTAGPPE
                     GPPGTEALAARTRSLGEGPPEAKPPLGDADGLVATEPGVVLAVLTADCVPV"
BASE COUNT          356 a          983 c          904 g          311 t
ORIGIN      
        1 ggccgtggcc gccatcgcca tcgccgtcgg cgtcgcctac ctgatccgca cgcccctgtt
       61 cggcctcagc gacgtccgga tcgagggcac ccgggcggtc accaaggccg aggtcgtcgc
      121 ggccagccag gtgcgcctgg gcgagcccta cctggggctc gacctggccg ccatccgcgc
      181 ccgggtggcc gccctgcccc gggtggcggc cgtgcgcgtg gcccgcgact acccctccag
      241 cctccgcatc accgtcagcg agcggccgcc ggtggccagc gtctccagcg gcagcgtgtt
      301 ctggctggtc gccgccgacg gcaccgtgct cgacgccgcc agccgccggc cggccgagct
      361 gccgtacgtg gccgcggtcc cgctgcccga cggcgtccgc cccggcagcc gcctgccccc
      421 cgggaacgag ctggccaacg ccctcaccgc cctcggcggc atggcccccc agctcaagcg
      481 gcaggtgacc ggcgtcaacg cccggtccct ggacagcctc gagttcacgc tccaggacgg
      541 ctcgcgggtc ctgtacgggc tcgcggtcga ccagccggca aaggacgccg ccgtgctgct
      601 ggtgcggcgg accctgaaac gagagggaag ggaggtgcag cgaatcgacg tccggaatcc
      661 gtcggcaccc accgtcagag cgacgccggc gaataacagc ggatgaaatt acaccagggt
      721 agttgaccag gtcaggggca ccttgtacca tccccgacag ctagaggttg acgtaatatt
      781 aacctcaact tgaggctcaa cttcccccgc ttccctggcc cttgcgctgg gcgacgactg
      841 tccgtgaaac gggcgaagga ggatggttgg atgatggcgg cgccgcagaa ctacctggcc
      901 gtgatcaagg tggtcgggat cggcggcggt ggagtcaacg ccgtcaaccg catgatcgag
      961 gcgagcctca agggcgtgga gttcatcgcc gtcaacaccg acgcccaggc cctgctcgcc
     1021 tccgacgccg acgtcaagct cgacgtgggc cgcgagctga cccgcgggct cggcgcgggc
     1081 tccaaccccg acgtcggccg ccaggccgcc gaggaccacg ccgaggagct cgaggaggtc
     1141 ctcaagggcg ccgacatggt gttcgtcacc gctggcgagg gcggcggcac cggcaccggg
     1201 ggcgccccgg tggtggcccg catcgccaag gcccaggggg cgctgaccat cggcgtggtg
     1261 accaggccgt tcgccttcga ggggaagcgc cgctccgccc aggccgacaa gggcatcgag
     1321 agcctcaagc aggaggtcga caccctcatc atcatcccca acgaccgcct gctccaggtg
     1381 gccgagcgca ccacctcgat gctggacgcc ttcaagctgg ccgaccaggt gctgctccag
     1441 ggcgtccagg ggatcaccga cctgatcacc atccccgggc tcatcaacct cgacttcgcc
     1501 gacgtgcgcg ccaccatgcg cgaggccggc accgccctca tgggcatcgg gcaggcccgg
     1561 ggcgaggacc gggccgtggc cgcggccaag caggccatct cctcgccgct gctggaggca
     1621 tcggtcgacg gggccagggg cgtgctcctc aacatctcgg gcggctccga cctgggcctg
     1681 ttcgaggtca acgaggccgc cgaggtgatc gcctcggccg cccaccccga cgccaacatc
     1741 atcttcgggg ccgtgatcga cgacgccctt ggcgacgagg tccgggtcac ggtgatcgcg
     1801 gccgggttcg accgcaccgc cgagcagacg gccgccgacg gcgggcgtcc ccaggcggcc
     1861 gggtcggccg aggacgagtc ggtctggccg acccctggca tgcggcgggc caacgacagc
     1921 cgcgtctccc gtccggcccc gccgacctcc tccccgggcc cggccgagcg gcgcgaacca
     1981 gagcggcggg agccggagcg gcgcgagcct gaccgccgcc cccggtacga cggcgatgac
     2041 gacgagctgg acatcccctc cttcctcaag cgctgacccg cagccacccg caggcatgct
     2101 ggcctggcgg cccggaccgc ccaccgcggg cgcgcccgcg tcggtgtggc cgcccgtgct
     2161 ggtggccgag gacctgctcg aggccggggt cgtggccgcc ttcaccggcc gggcgggcgg
     2221 cggctcgacg ccgccgtacg acaccctcaa cctgggcctg cgggtcgggg acgacctgcg
     2281 ccgggtcctg gccaaccgcc gccgggtcgc gaccgtcctc ggcctggccg gccgcccctg
     2341 ggccctggcc cgccaggtcc acggcgccga gatcctgcgg gtcaccgccg gcccgcccga
     2401 gggtccaccc ggaaccgagg ccctggccgc ccgcacccgg agcctcggcg aggggccgcc
     2461 tgaggccaag ccgccgctgg gcgacgccga cgggctggtc gccaccgagc ccggagtggt
     2521 gctggccgtg ctgacggccg actgcgtgcc cgtg
//
LOCUS       DASSWR010000288         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1140647, whole genome shotgun sequence.
ACCESSION   DASSWR010000288 DASSWR010000000
VERSION     DASSWR010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140647"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1935
                     /locus_tag="VFO47_04840"
     CDS             16..1935
                     /locus_tag="VFO47_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET8788053.1"
                     /translation="MLHGGRPWLGVNFWSRAGGPLMWRSYDPAVVCEELATLAAHGIN
                     LTRSFCYWPDFHPEPDRLDEAVLARFADFLDGHTEAGLATVPTFIVGHMSGQNWDPAW
                     RQGRDLYGDVWMVARQAWFVRRVTERFAGHPAVAGWLLSNEMPLYGGPAPAPVVTAWA
                     ELLVQAVRAGGGTQPVSLGDGAWGIELSGIDNGFSLRATAPLVDFVGPHVYRMETDRA
                     RLHLGPAFACELAGFAGKPVVLEEFGVSSGFSSSENAGHYYRQVLHTSLLAGATGWLA
                     WNNTDYDHLADQDPYRHHPFEMHFGLTDHTGAPKPHLAEVRAFAELLEAVDLPNLSRP
                     PAEAALVVASYLEHPYPFTEPADRVFVLDSLRQAYVAAWEADVAVRPMRERDGLEPGR
                     RLYLVPSAKQLTAPGWRALERFAGDGAVVYVSWSAGGHGVQRGPWYAGLDRMFGVEHQ
                     LTHGLVDPIEDEEVVVTFVRDFGSIPEGSRLELRAGGNQHVRAFLPVRPVTAEVVAVD
                     QQLRPVLLERATTGGGRLVLCTYPLEQLAAAAARVNPEPTWRIYDALATVAGLSRPVT
                     VEDPRVFAAELRHRDGRNFVWLVSQSDTEVTVKPTVKGHLADLQTGRPAEEVTLDAYG
                     VRVLRREPPTLVGPL"
     gene            1955..>3049
                     /locus_tag="VFO47_04845"
     CDS             1955..>3049
                     /locus_tag="VFO47_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8788054.1"
                     /translation="MRDKLRLAILVSVVCLLVAACTGNDSGGGSTSGQGQQPAGGTPA
                     AGPASYPRNETLYISGTQWGPPNNWNPIMDWQYATGTEGLVYENLFIYDPLKGEYKPW
                     LAEKGDWTSKTVYELTLRDGLTWSDGKPLTAADVVFTFELGKFASVPYHNLWDWLEKA
                     EATDERTVRFTFSEPLYQQWGNHLYNRAIVPKHLWEGRSEKDVATGANEKPVGSGPYL
                     YQTHSQDRMVWVKNDKWWGKTALSLDPKPKYIVDIVNSNNNAALGLVLQGGIDLSNNF
                     LPGIATLVKGGYQVQTYFPEPPYMLSANTAWLVLNTTKKPMDDPAFRKALAHAIDVDK
                     IVNGVYGQIVQAASPTGLLPAWDKYVDKSVV"
BASE COUNT          459 a         1140 c         1040 g          410 t
ORIGIN      
        1 agcgacgggg acaggctgct gcatgggggg cggccctggc tgggggtcaa cttctggtcg
       61 cgggccggcg ggccgctgat gtggcgcagc tacgaccccg cagtggtctg cgaggagctg
      121 gccaccctgg ccgcccacgg catcaacctc acccggtcgt tctgctactg gcccgacttc
      181 catcccgagc ccgaccggct cgacgaggcc gtgctggccc gcttcgccga cttcctggac
      241 ggccacaccg aggccggcct ggccaccgtg cccaccttca tcgtcggcca catgtcgggg
      301 cagaactggg acccggcctg gcggcagggg cgcgacctgt acggcgacgt ctggatggtg
      361 gcccgccagg cctggttcgt ccgccgggtc accgagcggt tcgccggcca cccggcggtg
      421 gccggctggc tgctgtccaa cgagatgccg ctgtacggcg gcccggcacc ggcgccggtg
      481 gtcacggcct gggcggagct gctggtgcag gccgtccggg ccgggggcgg gacccagccg
      541 gtgtcgctgg gcgacggcgc ctgggggatc gagctgagcg ggatcgacaa cggcttctcg
      601 ctgcgggcca ccgcgccgct ggtcgacttc gtcggccccc acgtctaccg gatggagacc
      661 gaccgggccc ggctgcacct gggaccggcc ttcgcctgcg agctggccgg cttcgccggc
      721 aagccggtgg tgctggagga gttcggcgtc agcagcggct tctcgtcgtc ggagaacgcc
      781 ggccactact accgccaggt gctccacacc agcctgctgg ccggggcgac cggctggctg
      841 gcctggaaca acaccgacta cgaccacctg gccgaccagg acccctaccg gcaccaccct
      901 ttcgagatgc acttcgggct gaccgaccac accggcgccc ccaagcccca cctggccgag
      961 gtccgcgcct tcgccgagct gctggaggcg gtcgacctcc cgaacctgtc gcgcccgccg
     1021 gccgaggccg ccctggtggt ggcctcctac ctcgagcacc cctacccgtt caccgagccc
     1081 gccgaccgcg tcttcgtgct cgactcgctc cgccaggcgt acgtggccgc ctgggaggcc
     1141 gacgtggccg tgcggccgat gcgcgagcgc gacggcctgg agcccggccg ccgcctctac
     1201 ctggtcccgt ccgccaagca gctcaccgcc ccgggatggc gggccctgga gcgcttcgcc
     1261 ggcgacggcg ccgtcgtcta cgtctcctgg agcgccggcg gccacggcgt ccagcgcggc
     1321 ccctggtacg ccggcctgga ccgcatgttc ggggtcgagc accagctcac ccacgggctc
     1381 gtcgacccca tcgaggatga ggaggtggtc gtcaccttcg tgcgcgactt cgggtcgata
     1441 ccggaaggca gccggctcga gctccgcgcc ggcggcaacc agcacgtccg cgcgttcctg
     1501 cccgtccggc cggtcaccgc cgaggtcgtc gccgtcgacc agcagctgcg gccggtgctg
     1561 ctggaacgcg ccaccaccgg cggcggccgg ctggtgctgt gcacctaccc gctggagcag
     1621 ctcgcggccg cggcggcccg ggtcaacccc gagccgacct ggcggatcta cgacgcgctg
     1681 gccaccgtgg ccggcctcag ccgcccggtg acggtggagg acccgcgggt cttcgccgcc
     1741 gagctgcgcc accgggacgg ccgcaacttc gtctggctgg tcagccagtc ggacaccgag
     1801 gtcaccgtca agccgacggt caagggccac ctggccgacc tccaaaccgg gcggcccgcc
     1861 gaggaggtca ccctcgacgc ctacggcgtc cgggtcctgc ggcgggagcc cccaaccctg
     1921 gtggggcccc tgtagaagga gaaaggggac tggaatgcga gacaagctca ggctggcgat
     1981 cctcgtctcg gtggtgtgcc tgctggtggc ggcatgcacc gggaacgact ccggtggcgg
     2041 atccacgtcc gggcagggcc agcagcccgc cggcgggacg ccggccgccg ggccggcgtc
     2101 ctacccccgc aacgagacgc tgtacatcag cgggacgcag tgggggccgc ccaacaactg
     2161 gaacccgatc atggactggc agtacgccac cgggaccgag gggctggtgt acgagaacct
     2221 gttcatctac gacccgctca agggcgagta caagccctgg ctggcggaga agggtgactg
     2281 gaccagcaag accgtctacg agctgacgct gcgcgacggc ctcacctgga gcgacggcaa
     2341 gcccctgacc gcggccgacg tcgtcttcac gttcgagctg ggcaagttcg cgtcggtgcc
     2401 gtaccacaac ctgtgggact ggctggagaa ggccgaggcg accgacgagc ggaccgtccg
     2461 cttcaccttc tccgagcccc tctaccagca gtggggcaac cacctctaca accgggcgat
     2521 cgtgcccaag cacctgtggg agggccgctc ggagaaggac gtcgccaccg gcgccaacga
     2581 gaagcccgtg ggcagcggcc cctacctgta ccagacccac agccaggacc ggatggtctg
     2641 ggtcaagaac gacaagtggt ggggcaagac cgccctcagc ctcgacccca agccgaagta
     2701 catcgtcgac atcgtcaact ccaacaacaa cgcggccctc ggcctggtcc tgcagggcgg
     2761 catcgacctc tccaacaact tcctcccggg gatcgccacc ctggtcaagg gcggctacca
     2821 ggtccagacc tacttccccg agccgccgta catgctgtcg gccaacaccg cctggctggt
     2881 cctgaacacc accaagaagc cgatggacga cccggccttc cgcaaggccc tggcccacgc
     2941 catcgacgtc gacaagatcg tcaacggcgt ctacggccag atcgtccagg cggccagccc
     3001 gaccggcctc ctccccgcct gggacaagta cgtcgacaag tcggtcgtc
//
LOCUS       DASSWR010000289         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_39244,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000289 DASSWR010000000
VERSION     DASSWR010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39244"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="VFO47_04850"
     CDS             <1..1784
                     /locus_tag="VFO47_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788055.1"
                     /translation="RLCREAVRLVSAAGRRELLVVLAIKVVNGVGLAVVLLLGKQVLD
                     GVIAAGRGGAGPGLVLPRLLLLTAVMAGLSFLSAIGREHREILAELASRYAQSQIIDV
                     ACAVELEAYETPAFHDRLVRAAGGGQFRPWQVVEGLTGLAGALVGIAGVTLALLALQP
                     WLVPLVLAAGVPLLVAVARGGELMFRFHHRTTEAERRRNYLYMLLSGKDAAKELRAFG
                     LEAFLRGRFDRLYDEHLAELRKVARRRLRVSLLGNLVTTAALGGIVAVLLVLALDGRI
                     GLADAGTAAGAVFVLGERLMNGVFSAGMLYESSLFIEDFTSFVAMAPEVEARRRRDPA
                     PSGFRRLQVEDVSFTYPSATSPALSGVSMEIGAGEIVALVGENGSGKTTLAKLLCRLY
                     LPHGGRVLWDGVDTAIVDPDELRRSVAVIFQDFLHYSLPASENIGMGRHQRLGDPEAI
                     RGAAVHAGADEFLARLPAGYETVLGPEFEGGHELSVGQWQRVALARAFFRDAPFIILD
                     EPTAALDARAEHELFESIRTLCRGRSVLLISHRFSSVRSADRIYVLDRGRVAESGSHG
                     ELMALGGRYAGLFTLQASAYLSPSRPS"
     gene            complement(1757..2236)
                     /locus_tag="VFO47_04855"
     CDS             complement(1757..2236)
                     /locus_tag="VFO47_04855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788056.1"
                     /translation="MRPEGMTEAITYRVAEAERPEVREFLSREWPLADRRLFGRELDW
                     TSRPVVVEARAGRDLAGVAVGEAVAGMARLHDLLVTGPRQNREVGRRLIELFCGRAAE
                     LGAARCFLRCPDTDRHRRFYERCGFVVVAVLPRYYHDHDFVEYVREPLLQEGRLGDR"
     gene            2300..3097
                     /locus_tag="VFO47_04860"
     CDS             2300..3097
                     /locus_tag="VFO47_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788057.1"
                     /translation="MDVRLTGVYIADARLVSEGVAQPEDAMAVFVGRAGKGRIAPFAV
                     RRSYTGAGGWYREAMAVLAPNGDVVWRSPEQALTLRGSSKIDTFTDEVHGVVVDSDDD
                     HELVLLVGGDEVGRVPMAVLDEDPPWSTGPDTAVLDEALKKSTVVWVDVPGEGGTDGR
                     AVPVWYGVLDGRVYVLVGGSEQHVPGLAEADRVVLIARSKEQQSLVAEVEASARVVPG
                     SDPLFAKVASVLLPRRLNLRDGEEAVDRWRKECTLVELTPSSIPAEV"
     gene            3165..>3406
                     /locus_tag="VFO47_04865"
     CDS             3165..>3406
                     /locus_tag="VFO47_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788058.1"
                     /translation="MPLRKSKESKATKAKEVVTDQVSERTDDLAAALEAAREAIARAT
                     NAASRKGAELGAELGKEATKAADKLMPERARQRRREA"
BASE COUNT          437 a         1252 c         1254 g          463 t
ORIGIN      
        1 cccggctgtg ccgggaggcg gtccggctcg tctcggccgc ggggcgccgc gagctgctgg
       61 tcgtcctggc catcaaggtg gtcaacgggg tcggcctggc cgtggtcctg ctgctcggca
      121 agcaggtgct cgacggggtg atcgccgccg gtcgcggcgg cgccgggccc ggcctggtgc
      181 tgccgagact gctgctgctg acggcggtca tggccgggct gagcttcttg tcggccatcg
      241 gccgggagca ccgcgagatc ctggccgagc tggcctcccg gtacgcccag tcccagatca
      301 tcgacgtggc ctgcgcggtc gagctggagg cctacgagac cccggccttc catgaccggc
      361 tggtccgcgc ggccggcggc ggccagttcc ggccatggca ggtcgtcgag ggcctgaccg
      421 gcctggccgg cgccctggtc ggcatcgccg gggtcaccct ggccctgctc gccctccagc
      481 cctggctggt gccgctggtc ctggccgctg gcgtccccct gctggtcgcc gtggccaggg
      541 gcggcgagct gatgttccgc ttccaccacc ggacgaccga ggccgagcgc cgccgcaact
      601 acctctacat gctgctgtcg ggcaaggacg cggccaagga gctgcgcgcc ttcggtctcg
      661 aggcgttcct gcgcgggcgc ttcgaccggc tgtacgacga gcacctggcc gagctgcgga
      721 aggtcgccag gcggcggctg cgcgtgtcgc tgctcggcaa cctggtcacc accgcggcgc
      781 tcggcgggat cgtcgccgtc ctgctggtcc tggccctcga cggccgcatc gggctggccg
      841 acgccgggac ggccgccggg gccgtgttcg tcctcggcga gcggctgatg aacggcgtct
      901 tcagcgccgg gatgctctac gagtcctcgc tcttcatcga ggacttcacc tcgttcgtgg
      961 ccatggcccc ggaggtggag gcacggcggc ggcgggaccc ggcaccgtcc ggcttccggc
     1021 ggctccaggt cgaggacgtc agcttcacct acccgagcgc caccagcccg gccctgagcg
     1081 gcgtgtccat ggagatcggg gccggcgaga tcgtcgccct ggtcggcgag aacggctcgg
     1141 gcaagaccac tctggccaag ctgctgtgcc ggctctacct gccccacggc ggccgcgtgc
     1201 tctgggacgg ggtcgacacc gcgatcgtcg accccgacga gctgcgccgc tcggtggccg
     1261 tcatcttcca ggacttcctc cactattcgc tgccggcgtc ggagaacatc ggcatgggcc
     1321 gccaccagcg gctcggcgac cccgaggcga tccggggggc ggcggtgcac gccggcgccg
     1381 acgagttcct ggccaggctg cccgccggct acgagaccgt gctcgggccc gagttcgagg
     1441 ggggccatga gctctcggtc ggccagtggc agcgggtcgc gctggccagg gcgttcttcc
     1501 gcgacgcccc gttcatcatc ctcgacgagc cgaccgcggc cctggacgcc cgggccgagc
     1561 acgagctgtt cgagagcatc cggaccctgt gccggggccg gtcggtgctg ctgatctcgc
     1621 accggttctc cagcgtgcgc tcggccgacc gcatctacgt cctggaccgg ggccgggtgg
     1681 ccgagtcggg cagccacggc gagctgatgg ccctcggcgg ccgctacgcc gggctgttca
     1741 ccctgcaggc ctccgcctac ctgtcgccca gccggccctc ctgaaggagc ggctcgcgca
     1801 cgtactcgac gaagtcgtgg tcgtggtagt agcggggcag cacggccacg accacgaacc
     1861 cgcagcgctc gtagaagcgg cggtggcggt cggtgtcggg gcagcgcagg aagcagcggg
     1921 ccgcgcccag ctcggcggcg cggccgcaga acagctcgat caggcggcgg ccgacctccc
     1981 ggttctggcg cgggccggtg accagcaggt cgtggaggcg ggccatgccg gcaaccgcct
     2041 cgccgaccgc gaccccggcc aggtcccgcc cggccctggc ctcgaccacc accgggcggc
     2101 tggtccagtc cagctcccgg ccgaacaggc ggcggtcggc cagcggccac tcccgggaca
     2161 ggaactcccg cacctccggg cgctcggcct cggccacccg gtaggtgatc gcctccgtca
     2221 tcccctccgg tcgcatgggc acatcttcta tgatcggtgc cgccgcagcc gcagccagat
     2281 gggatcggaa aggaaccgga tggacgtccg cctcaccggc gtctacatcg ccgacgccag
     2341 gctggtctcc gagggggtgg cccagcccga ggacgccatg gccgtgttcg tcggccgggc
     2401 cggcaagggg cggatcgccc cgttcgccgt ccgccgctcc tacaccggcg ccggcggctg
     2461 gtaccgcgag gccatggccg tgctcgcccc caacggcgac gtcgtctggc gcagcccgga
     2521 gcaggccctg accctgcgcg gctccagcaa gatcgacacc ttcaccgacg aggtccacgg
     2581 cgtggtcgtg gactccgacg acgaccacga gctggtcctg ctggtcggcg gcgacgaggt
     2641 cgggcgcgtt cccatggccg tgctcgacga ggacccgccc tggtcgaccg gccccgacac
     2701 ggccgtgctc gacgaggcgc tcaagaagag cacggtggtc tgggtcgacg tccccggcga
     2761 gggcgggacc gacggccggg ccgtgcccgt ctggtacggc gtcctcgacg gccgcgtcta
     2821 cgtgctcgtc ggcgggtccg agcagcacgt ccccgggctg gccgaggcgg accgggtggt
     2881 cctgatcgcc cgctccaagg agcagcagtc gctggtcgcc gaggtggagg cgtccgcccg
     2941 ggtggtcccg gggtccgacc cgctgttcgc caaggtcgct tccgtgctgc tcccccgccg
     3001 cctcaacctg cgcgacgggg aggaggcggt ggaccgctgg cgcaaggaat gcaccctggt
     3061 cgagctcacc ccctcgtcga tcccggcgga ggtgtagggg catccggggc gggtaggcac
     3121 accaaaccgt gccaaagcag ccaacgagcc cgagggaggg ccgcatgcca ctccgcaagt
     3181 ccaaggaatc aaaggcgacc aaggccaagg aggtcgtcac cgaccaggtc agcgagcgga
     3241 ccgacgacct ggcggccgcg ctcgaggcgg ccagggaggc catcgcccgg gccaccaacg
     3301 cggccagccg caagggggcc gagctcggcg ccgagctggg caaggaggct accaaggccg
     3361 ctgacaagct gatgccggag cgggcccgcc agcgccgccg cgaggc
//
LOCUS       DASSWR010000290         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_435270,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000290 DASSWR010000000
VERSION     DASSWR010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435270"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFO47_04870"
     CDS             <1..655
                     /locus_tag="VFO47_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788059.1"
                     /translation="TVAMVVVAAGLFGSAPDQLPVAARRSPPTQDLSHGVGEVRVPVL
                     LPENRDRVVRRQASCPRLDRVTLVGTAAEVALLDAAADKLCALRSTAPIQRARAGLQE
                     AAATLAFAEFELSINESATDFGPERPAVLVHAKFQQGEAAPERVAAVLAHEGAHVAGG
                     GPPDAAAELAARTAELEACGRLFGGDVQPNRGCADAAALLSGDDASALARLKEAGYR"
     gene            652..1491
                     /locus_tag="VFO47_04875"
     CDS             652..1491
                     /locus_tag="VFO47_04875"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8788060.1"
                     /translation="MTLELDWPVGRAGVAVVSPSGVETAFLGGVTGDDRFEVASVTKL
                     MTSLAALAAVESGRIELDQPVPGGGEGVTLRHLLAHAGGHPFEPPGRARPPKQRRIYS
                     NVGFRILAEAVADAAGTTFASWLSTSVLDPLGMTATGLVHRRGVDGDPAAGAASTLDD
                     LTRLARCLLDRGAPVVGPDLFAEAISVQFPGLAGLVPGVGRFDPCDWGLGFELHDSKR
                     PHWMGDRRSPSAFGHFGASGCFLWVDPDASLAAAAVTDRDFDDDKWAMATWPGWSDRL
                     PAA"
     gene            1654..2313
                     /gene="purQ"
                     /locus_tag="VFO47_04880"
     CDS             1654..2313
                     /gene="purQ"
                     /locus_tag="VFO47_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET8788061.1"
                     /translation="MKVGVVTFPGSCDDRDARAAVAAMGGEPLALWHGDGDLRGVDAV
                     VLPGGFSYGDYLRCGAIARFSPVMGAVAAFARSGGPVLGICNGFQVLCEAGLLPGALL
                     RNAGLRFVCREVEVVVESTATAWTGACTAGESLAIPVKHGEGRYVCDDPAALAAAGQV
                     VFRYAPGHNPNGSMADIAGVRNPAGNVVGLMPHPEHAVDPLLGPVGGRGLFESVLAAR
                     V"
     gene            2306..>3218
                     /locus_tag="VFO47_04885"
     CDS             2306..>3218
                     /locus_tag="VFO47_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET8788062.1"
                     /translation="MSSTAATLHRQLGLSDEEFERILAVLGREPSRAELAMFSLLWSE
                     HCSYKSSKVHLRRLPTKGARVLQGPGENAGVVDLGDGLAAVFKIESHNHPSFVEPFQG
                     AATGVGGIIRDILAMGARPAASLNSLRFGDPAEPLQRRLVEGVVAGIGHYGNCVGVAT
                     VGGEVVFDPCYAGNPLVNALTIGFMPADRRLSGSAERPGDLAVLMGAKTGRDGIGGAS
                     VLASASFEEGDEAKRPNVQVGDPFQEKLLIEACLELVERGLLRGLQDLGAAGISCAVA
                     EVAARAGMGMEVDLDAVSLREPSMEAWE"
BASE COUNT          352 a         1215 c         1228 g          423 t
ORIGIN      
        1 gaccgtggcc atggtggtgg tggcggccgg cctgttcggg tcggcccccg accagctccc
       61 ggtcgcggcc cggcgctcgc ccccgaccca ggacctgagc cacggcgtcg gcgaggtccg
      121 cgtcccggtc ctcctgcccg agaaccggga ccgggtcgtg cggcgccagg ccagctgccc
      181 ccgcctggac cgggtcaccc tggtcggcac ggccgccgag gtggcgctgc tggacgcggc
      241 cgccgacaag ctctgcgccc tgcgctcgac cgccccgatc cagcgggcgc gcgccgggct
      301 ccaggaggcg gccgccacgc tggccttcgc cgagttcgag ctgtccatca acgagtcggc
      361 caccgacttc gggccggagc ggccggcggt cctggtccac gccaagttcc agcagggcga
      421 ggccgcgccc gagcgggtcg cggccgtgct ggcccacgag ggcgcccacg tcgccggcgg
      481 cgggccgccg gacgcggccg ccgagctggc cgcccgcacg gccgagctgg aggcctgcgg
      541 ccggctgttc ggcggcgacg tccagcccaa ccggggctgc gcggacgccg ccgccctgct
      601 gtcgggcgac gacgcgagcg cgctggcgcg actgaaggag gcggggtacc ggtgacactg
      661 gagctggact ggccggtcgg gcgggccggg gtggcggtgg tctcgccgtc gggggtggag
      721 acggccttcc tcggcggagt gaccggcgac gaccggttcg aggtggcgtc ggtgaccaag
      781 ctgatgacct cgctggccgc cctggccgcg gtcgagtcgg ggcggatcga gctcgaccag
      841 ccggtccccg gcggcggcga gggcgtcacc ctccgccacc tgctggccca tgccggcggc
      901 catcccttcg agcccccggg ccgggcccgg ccccccaagc agcgccgcat ctactccaac
      961 gtcggcttcc ggatcctggc cgaggcggtc gccgacgccg ccggcacgac cttcgccagc
     1021 tggctctcca ccagcgtgct cgatccgctg ggcatgaccg cgaccgggct cgtccaccgc
     1081 cgcggggtcg acggcgaccc ggccgccggc gccgcctcca ccctcgacga cctgacccgc
     1141 ctggcccgct gcctgctgga ccggggcgcc cccgtggtcg gccccgacct gttcgccgag
     1201 gccatctccg tccagttccc cggcctggcc gggctggtcc ccggggtcgg ccgcttcgac
     1261 ccctgcgact ggggcctcgg cttcgagctc cacgacagca agcgccccca ctggatgggc
     1321 gaccgccgct cgccctcggc cttcggccac ttcggggcca gcggctgctt cctctgggtc
     1381 gatcccgacg ccagcctggc cgccgccgcc gtcaccgacc gcgacttcga cgacgacaag
     1441 tgggccatgg ccacctggcc cggatggtcg gaccgcctcc ccgcggcctg agggcgcgcc
     1501 gcaccccggc cgcctggtcg gcgctcggcc ccccggaggg gagcgtagcc ggtcgccaag
     1561 cgagctgccc atcgcggtcc acagccgcaa atcatcggca atgctgtctg gtagcctgcg
     1621 tcgcggtcgg gacgtcgtcg gggaggctgc cgcatgaagg tcggggtggt aaccttcccg
     1681 gggtcgtgtg acgaccggga cgcccgggcc gccgtggccg cgatgggggg cgagccgctg
     1741 gccctgtggc acggcgacgg cgacctccgg ggggtcgacg ccgtggtcct gcccggcggg
     1801 ttctcctacg gcgactacct gcgctgcggg gcgatcgccc ggttctcccc ggtgatgggg
     1861 gcggtggccg ccttcgcccg ctccggcggg ccggtccttg gcatctgcaa cggcttccag
     1921 gtgctgtgcg aggccggcct gctccccggg gccctgctcc gcaacgccgg gctgcgcttc
     1981 gtctgccgcg aggtcgaggt ggtggtcgag tccaccgcca ccgcctggac cggcgcctgc
     2041 accgccggcg agtccctggc catccccgtc aagcacggcg agggccgcta cgtctgcgac
     2101 gacccggccg ccctggccgc cgccggccag gtcgtgttcc gctacgcccc cggccacaac
     2161 cccaacggct ccatggccga catcgccggg gtccgcaacc ccgccggcaa cgtggtcggc
     2221 ctcatgcccc accccgagca cgccgtcgac cctctgctcg gcccggtcgg cggccggggg
     2281 ctgttcgagt cggtcctggc ggcccgtgtc tagcaccgcg gccaccctgc accgccagct
     2341 cggcctgagc gacgaggagt tcgagcggat cctggcggtg ctggggcggg agccgagccg
     2401 ggccgagctg gccatgttct cgctgctgtg gtccgagcac tgctcctata agtcgtccaa
     2461 ggtgcacctg cggcggctgc ccacgaaggg ggcgagggtg ctgcaggggc cgggggagaa
     2521 cgccggggtg gtcgacctgg gcgacgggct ggcggcggtg ttcaagatcg agtcccacaa
     2581 ccacccgtcg ttcgtggagc cgttccaggg ggcggccacc ggggtcgggg ggatcatccg
     2641 cgacatcctg gccatggggg cgcggccggc ggccagcctc aactcgctgc ggttcggcga
     2701 cccggccgag ccgctgcagc ggcggctggt cgagggggtg gtggccggga tcggccacta
     2761 cggcaactgc gtcggggtgg ccaccgtcgg gggcgaggtc gtgttcgacc cctgctacgc
     2821 cggcaacccg ctggtcaacg ccctcaccat cggcttcatg ccggccgacc ggcgcctcag
     2881 cggctcggcc gagcggccgg gtgacctggc cgtgctgatg ggagccaaga ccggccggga
     2941 cgggatcggc ggggcgagcg tgctcgcctc ggccagcttc gaggagggcg acgaggccaa
     3001 gcgcccgaac gtccaggtgg gcgacccgtt ccaggagaag ctgctgatcg aggcctgcct
     3061 ggagctggtc gagcggggcc tgctgcgcgg gctccaggac ctgggggcgg ccgggatcag
     3121 ctgcgcggtc gccgaggtcg cggcccgggc cgggatgggc atggaggtcg acctggacgc
     3181 cgtgtccctg cgcgagccgt cgatggaggc ctgggagg
//
LOCUS       DASSWR010000291         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1226906, whole genome shotgun sequence.
ACCESSION   DASSWR010000291 DASSWR010000000
VERSION     DASSWR010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226906"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFO47_04890"
     CDS             complement(<1..223)
                     /locus_tag="VFO47_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017994084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET8788063.1"
                     /translation="MTSLGAASTGMVCPACGVASPAEARFCPSCGQRLLGGVDERRVV
                     TVLFADLVDSTRLVESLDPEAARDLLNETL"
     gene            complement(263..472)
                     /locus_tag="VFO47_04895"
     CDS             complement(263..472)
                     /locus_tag="VFO47_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788064.1"
                     /translation="MSSMEPDEGTQADAPRLDRLEAADAADAPRLDTAEATGGSDAPR
                     LDRAPRLDAAGAGSAEDDPDDSPDS"
     gene            complement(572..1753)
                     /locus_tag="VFO47_04900"
     CDS             complement(572..1753)
                     /locus_tag="VFO47_04900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8788065.1"
                     /translation="MAGASVVVVGGGVMGCSIAFHLAERGVDVLVLERGTVCSGMTAR
                     SGALVRMHYTNEPEARMALTGLRYFQDWKDRVGGWCGFTVTGAAVLVGPGEGERLRRN
                     VAMLRRVGVDTEAVAPADLAAEHPELDLAGVEMAAVEPGSGYADPVATTFAFASRAVD
                     LGARIGQGVAVRGLAVEGDRVVGVDTDGGRVGADAVVLACGPWVDPLARTAGFELGIT
                     PERSQIAFFRRPGAARRHPVVIDGVLGTYFRPHGGELSLLGVAAGHHIEVAAIDREVE
                     GYDHEVVAPALGRLAGRVPSFAGAPFARGHRGVYDTSADSRAVLDAAPGVAGLFVAGG
                     FSGTGFKKSPAVGACMAELVTQGAATTVDIRPFRLGRFAEGDPVVGDEYRLPPEFGHK
                     L"
     gene            1796..2563
                     /locus_tag="VFO47_04905"
     CDS             1796..2563
                     /locus_tag="VFO47_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8788066.1"
                     /translation="MRIKLVSDIHGAADALAREAAGADVLLVCGDLVNLIDYRTMEGI
                     AAEVFGPEATRDFVRHRTAGRFEEAGRVLRSASAGREDEVRDRVRAAVRAQYEAVFAA
                     FPEPTYLTHGNVDRPEQFQDLLRPGIRHLDGETVEVDGMRVGFVGGGLPRGERPHLSE
                     ITHEAFAAKVAAVGPVDVLCSHMPPAVDDLRFDVVAGRPEPGSQALLDYVEEHQPDYL
                     YFGHVHQPRRSRLRVGRTWLVNVGYFRGTGRMLEHPA"
     gene            2576..3013
                     /locus_tag="VFO47_04910"
     CDS             2576..3013
                     /locus_tag="VFO47_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8788067.1"
                     /translation="MAEHAEGSTEVYATPAEVMAVVADFEAYPEWVGALEEVEVLARD
                     RRGRGTRVAFRLGTPVGDQAYTLAYRYAPRDAGMSWTYVEGTLDDLAGSYALEPGGDG
                     ATRVTYRLEVALGVPLPGLIKRQAAKQIVRSALSDLKRRVESM"
     gene            3026..4228
                     /locus_tag="VFO47_04915"
     CDS             3026..4228
                     /locus_tag="VFO47_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HET8788068.1"
                     /translation="MRILLFTGKGGVGKTTVAAATAARAAALGHRTLVMSTDPAHSLA
                     DSFDVELGAEPRQVADRLWAEQIDSQARLEANWRDIQEYVVNLLNWGGVDAVEAEELS
                     VIPGLDELFSLIDINRYHQAGDYDLLVVDCAPTAETLRLLSLPEALGWYIDRLFPSSR
                     RIATVARPLLGRLGNLPPIASDAVFAAVERLYRNLERVRVLLAEPETSTVRLVLNPEK
                     MVVAEARRTYTYLSLFGYRVDAVVVNRLLPDDVTDPYFGKWKEIQADHFRTITESFSP
                     LPILTCRLFDDEMVGLERLSEVAAAVYGSRDPAGVLAESRPLRVTKQAGGGYALRMDL
                     PFAERSDLDVHRKGDELLIKVGSFKRNVLLPQALQPLEISAANLRRGTLVVRFDAREG
                     PSADARGA"
BASE COUNT          579 a         1658 c         1574 g          545 t
ORIGIN      
        1 ggagggtctc attcagcagg tcccgcgcgg cctccgggtc gagcgactcc accagcctgg
       61 tcgagtcgac caggtcggcg aagaggaccg tcaccacccg gcgctcgtcg acaccgccga
      121 gcagccgctg gccgcaggac gggcagaagc gcgcctcagc cggcgacgcc accccgcagg
      181 cggggcagac catgccggtc gaagcggctc cgagtgaagt cacggccctc acctagacgg
      241 cggcgtcgga gcgccggtgg gatcaggagt cggggctgtc gtccgggtcg tcctcggcgg
      301 agccggcgcc ggccgcatcg aggcggggag ccctgtccag gcggggagcg tcgctgccgc
      361 cggtcgcctc ggccgtgtcc aggcggggcg cgtcggccgc gtcggcggcc tcgagcctgt
      421 ccaggcgcgg ggcgtcggcc tgggtgccct cgtcgggctc catgctgctc atggtccctc
      481 cctcagggtt cgcgatggat cgctcaagga gtctatatgg cgaggccagc cgattgatcc
      541 cttccggccc atcccgaacg attgccgcgg gctacagttt gtgaccgaac tccggcggca
      601 ggcggtactc gtccccgacg accgggtcgc cctcggcgaa gcggcccagg cggaacgggc
      661 ggatgtcgac ggtggtggcc gcgccctggg tcaccagctc ggccatgcag gcgccgacgg
      721 cgggggactt cttgaacccg gtgccggaga acccgccggc cacgaacagc ccggccaccc
      781 cgggggcggc gtcgagcacg gcccggctgt cggccgaggt gtcgtagacg ccgcggtggc
      841 cgcgtgcgaa cggcgccccg gcgaacgacg gcacccgccc ggccaggcgc ccgagggccg
      901 gggcgaccac ctcgtggtcg tagccctcga cctcccggtc gatcgccgcc acctcgatgt
      961 ggtggccggc cgccaccccg agcagcgaca gctcgccccc gtggggccgg aagtaggtgc
     1021 cgagcacccc gtcgatcacc accgggtgcc ggcgggcggc cccgggccgg cggaagaacg
     1081 cgatctggga gcgctcgggc gtgatcccca gctcgaaccc ggccgtccgg gccagcgggt
     1141 cgacccacgg cccacaggcg agcacgaccg cgtcggcccc gacccgcccg ccgtcggtgt
     1201 cgaccccgac cacccggtcg ccctccacgg ccagcccgcg cacggccacc ccctggccga
     1261 tccgcgcccc caggtccacc gcccgggacg cgaacgcgaa ggtggtcgcc accgggtcgg
     1321 cgtagccgga gccgggctcg accgccgcca tctccacccc ggccaggtcc agctcgggat
     1381 gctcggcggc caggtcggcc ggggccacgg cctcggtgtc gaccccgacc cgccgcagca
     1441 tggccacgtt gcggcgcagc cgctcgccct ccccgggccc gaccagcacc gccgccccgg
     1501 tgacggtgaa cccgcaccag cccccgaccc ggtccttcca gtcctggaag tagcgcagcc
     1561 cggtcagggc catccgggcc tccggctcgt tggtgtagtg catccgcacc agcgcccccg
     1621 accgggccgt catccccgag cagaccgtgc cccgttccag cacgagcacg tcgacgcccc
     1681 gctcggccag gtggaaggcg atcgagcagc ccatcacccc gcccccgacc accaccacgc
     1741 tcgccccagc catgagggtc agcctacgcg aggcgcgccg gtagcatggg cggacatgcg
     1801 gatcaagctc gtctcggaca tccacggggc cgccgacgcg ctggcgcggg aggccgccgg
     1861 ggcggacgtg ctgctggtct gcggcgacct ggtgaacctg atcgactacc gcacgatgga
     1921 ggggatcgcg gccgaggtgt tcgggccgga ggcgacccgg gacttcgtgc ggcaccgcac
     1981 cgccgggcgg ttcgaggagg cggggcgggt gctgcgcagc gcctcggcgg ggcgggagga
     2041 cgaggtccgc gaccgggtcc gggcggccgt ccgggcccag tacgaggcgg tgttcgccgc
     2101 cttccccgag cccacctacc tgacccacgg caacgtcgac cgtcccgagc agttccagga
     2161 cctgctccgg cccgggatcc ggcatctgga cggggagacg gtcgaggtcg acgggatgcg
     2221 ggtcgggttc gtgggcggcg ggctgccgcg gggggagcgg ccgcacctgt cggagatcac
     2281 ccacgaggcg ttcgcggcca aggtggccgc cgtcggcccg gtcgacgtgc tctgctcgca
     2341 catgcccccg gccgtcgacg acctgcgctt cgacgtcgtc gccggccggc ccgagccggg
     2401 cagccaggcg ctgctcgact acgtggagga gcaccagccc gactacctct acttcggcca
     2461 cgtccaccag ccacggcgca gccgcctccg ggtgggccgg acctggctgg tcaacgtcgg
     2521 ctacttccgg ggcaccgggc ggatgctgga gcacccggcg taataccatc ggggcatggc
     2581 cgagcatgcc gaagggtcca ccgaggtgta cgccaccccc gcggaggtga tggcggtggt
     2641 ggccgacttc gaggcctatc cggagtgggt cggcgccctc gaggaggtcg aggtcctggc
     2701 ccgcgaccgg cgggggcggg ggaccagggt cgccttccgg ctcggcaccc cggtggggga
     2761 ccaggcctac accctggcct accggtatgc gccacgggat gcgggcatgt cctggacgta
     2821 cgtggagggg acgctggacg acctggccgg ctcgtacgcc cttgagccgg gcggcgacgg
     2881 cgccaccagg gtcacctacc ggctcgaggt cgccctcggc gtccccctgc ccggcctgat
     2941 caagcggcag gcggccaagc agatcgtccg ctcggccctg agcgacctga agcgccgcgt
     3001 ggagtccatg taggctgcca gcaccgtgcg catcctcctg ttcaccggca agggcggcgt
     3061 gggcaagacc accgtggccg cggccacggc ggcccgggcg gcggccctcg ggcaccgcac
     3121 cctggtgatg tccaccgacc cggcccattc gctggccgac tccttcgacg tcgagcttgg
     3181 cgccgagccg cgccaggtcg ccgaccggct gtgggccgag cagatcgaca gccaggcccg
     3241 gctggaggcc aactggcgcg acatccagga gtacgtcgtc aacctgctca actggggcgg
     3301 ggtcgacgcc gtcgaggccg aggagctgtc ggtcatcccc ggcctggacg agctgttcag
     3361 cctcatcgac atcaaccgct accaccaggc cggcgactac gacctgctgg tcgtcgactg
     3421 cgccccgacc gccgagaccc tgcggctgct gtcgctgccc gaggccctcg gctggtacat
     3481 cgaccggctg ttcccgtcct cgcggcggat cgcgaccgtg gcccggccgc tgctgggccg
     3541 gctcggcaac ctgcccccga tcgcgtcgga cgccgtgttc gcagccgtgg aacggctgta
     3601 ccgcaacctg gagcgggtcc gggtgctgct ggccgagccc gagacctcga ccgtgcggct
     3661 ggtcctcaac cccgagaaga tggtcgtcgc cgaggcccgg cgcacctaca cctacctgtc
     3721 gctgttcggc taccgggtgg acgcggtggt ggtcaaccgg ctgctgcccg acgacgtcac
     3781 cgacccctac ttcggcaagt ggaaggagat ccaggccgac cacttccgca ccatcaccga
     3841 gtcgttctcg cccctgccca tcctcacctg ccggctgttc gacgacgaga tggtcgggct
     3901 ggagcggctg tcggaggtgg ccgccgccgt ctacggctcc cgcgacccgg ccggcgtcct
     3961 cgccgagtcg cggccgctgc gggtcaccaa gcaggccggc ggcggctacg ccctgcgcat
     4021 ggacctgccg ttcgccgagc ggagcgacct ggacgtgcac cgcaagggcg acgagctgct
     4081 gatcaaggtg ggcagcttca agcgcaacgt gctcctgccc caggccctgc agccgctgga
     4141 gatctccgcc gccaacctgc gccgcggcac cctggtggtg cgcttcgacg cccgcgaggg
     4201 gccgtccgcc gacgccaggg gcgcctgatg cccgcgccga ccggaggaac gcccatgccg
     4261 ccccacgacg ccgcggccgg ccaggccccc gagtgccagt ggtgcccgat ctgctcggcc
     4321 atcgcggccc tgcgcgaggc ccggccggag gcggtc
//
LOCUS       DASSWR010000292         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_661412,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000292 DASSWR010000000
VERSION     DASSWR010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661412"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="lnt"
                     /locus_tag="VFO47_04920"
     CDS             <1..924
                     /gene="lnt"
                     /locus_tag="VFO47_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET8788069.1"
                     /translation="FVQPGVVGPVEERFRASERLSRTLGPAAGVDLVVWGESSVGRDP
                     AANPGDVARLAAAARAAGGPVLANVDAQREAAGGIYKSALLVGPSGVLGSYDKMRLVP
                     FGEYVPLRPLLGWVTRLTDAAAVDRHRGARLAVLDAGGLAVGPLVCFESAFPDLSRNL
                     AAGGAELVVIQTADTTFQGSWGLDQHASLAAVRAVESGRPVLQGALSGTSAAFDARGR
                     RLAWAPGSWRGAAVAELPLSAERTPYVRAGDVVPVACGVALAAGAAAAWSRRVSRGGS
                     GRRTGSTPRSTPDRPRRRAAGTSRTAGRPGS"
     gene            complement(812..1642)
                     /locus_tag="VFO47_04925"
     CDS             complement(812..1642)
                     /locus_tag="VFO47_04925"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET8788070.1"
                     /translation="MSARQQAQRAERTAKGAAESGPVKTLGRVGLIAYGVVHVLVAYL
                     AVRVALGGGAKADKTGALQTIAEQPAGRFFLWVLTVGLVALTIWQLAEAGWGHGYRRP
                     ARRRTLQRLVSLGEAGVAGALAYSSFKVASGKKGNSKQEKAAFIDKVFDWPAGELLVA
                     AVGLGIVAIAVYLVHRGVAKTFVDDLELAGAEPHARDAAIRFGQVGWPALGVAYGILG
                     FLLIYAAVTYDPKKATGMDTALKTLAGQPYGTFLLLVVAAGLACFGAYCLFDARYRRG
                     "
     gene            complement(1720..>2695)
                     /locus_tag="VFO47_04930"
     CDS             complement(1720..>2695)
                     /locus_tag="VFO47_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8788071.1"
                     /translation="ETDPGAVTNNEHGRQADTPTEIPPKGWKDIAKRTAKEVKQDQVP
                     LLGAGVAFYILLSLFPAIIAGVSLYGLVADPQTVRDQIDRLAQRLSPETAKLVGDQIE
                     QITSGAGGALGVATVIGILTALWSASSGMKALITGVNLAYDESESRKFVKLRGRAIVM
                     TLGAMGLLAVAGATIVGFPPIANDLPVVVQWVVSILRFLLLAALLIVGLAALYRFAPD
                     RDEPRWTWVTWGSGVATVLWIVASIGFSIYANSFGNYNKTYGALAGVIILMFWLYLTA
                     VIVLVGAELNTEMELQTARDTTKGPEQPMGERGGHAADHVAESPAGGS"
BASE COUNT          337 a          989 c         1007 g          362 t
ORIGIN      
        1 ttcgtccagc ccggggtcgt cggcccggtc gaggagcggt tccgggccag cgagcggctc
       61 agccgcaccc tcgggccggc cgcgggcgtc gacctggtgg tgtggggcga gagcagcgtc
      121 ggccgcgacc cggcggccaa ccccggcgac gtggcccgcc tggccgccgc cgccagggcc
      181 gccggcgggc cggtcctggc caatgtcgac gcccagcggg aggcggccgg cgggatctac
      241 aagagcgccc tgctggtcgg cccgtccggg gtcctgggca gctacgacaa gatgcggctg
      301 gtgccgttcg gggagtacgt gccgctgcgg ccgctgctcg gctgggtgac ccgcctcacc
      361 gacgccgccg ccgtcgaccg gcaccgcggc gcccgcctgg ccgtgctgga cgccggaggc
      421 ctggccgtcg gcccgctggt ctgcttcgag tcggccttcc ccgacctgag ccggaacctg
      481 gccgccggcg gagccgagct ggtggtgatc cagacggccg acaccacctt ccagggcagc
      541 tggggccttg accagcacgc cagcctggcc gcggtgcggg cggtggagtc gggccggccg
      601 gtgctccagg gggcgctcag cgggacctcg gccgccttcg acgcccgcgg ccgccgcctc
      661 gcctgggcgc cggggagctg gcggggcgcg gccgtggccg agctgcccct gtcggccgag
      721 cgcaccccgt acgtgcgggc cggcgacgtc gtccccgtcg cctgcggggt cgccctggcc
      781 gccggggcgg ccgccgcctg gagccgccgg gtcagccgcg gcggtagcgg gcgtcgaaca
      841 ggcagtacgc cccgaagcac gccagaccgg ccgcgacgac gagcagcagg aacgtcccgt
      901 acggctggcc ggccagggtc ttgagggcgg tgtccatgcc ggtggccttc ttggggtcgt
      961 aggtgaccgc cgcgtagatc agcaggaacc ccaggatccc gtaggcgacc ccgagggccg
     1021 gccagcccac ctgcccgaag cggatggcgg cgtcgcgggc gtgcggctcg gcgccggcca
     1081 gctccaggtc gtccacgaag gtcttggcca cgccccggtg gaccaggtac acggcgatgg
     1141 ccacgatccc cagcccgacg gcggcgacca gcagctcccc cgccggccag tcgaagacct
     1201 tgtcgatgaa ggccgccttc tcctgcttgg agttgccctt cttgcccgag gcgaccttga
     1261 aggagctgta ggccagcgcc ccggcgaccc cggcctcgcc caggctgacc agccgctgca
     1321 gggtgcgccg gcgggccggc cgccggtagc cgtggcccca gccggcctcg gccagctgcc
     1381 agatggtcag ggcgaccagg ccgacggtga gcacccagag gaagaaccgg cccgccggct
     1441 gctcggcgat cgtctgcagg gcgccggtct tgtcggcctt ggccccgccg cccagggcga
     1501 cccggacggc caggtaggcg accagcacgt ggacgacccc gtaggcgatc agcccgaccc
     1561 ggccgagggt cttgaccggt ccgctctcgg ccgcgccctt cgccgtccgc tccgcccgct
     1621 gtgcctgctg ccgtgcgctc atccgcctgc tccccggttg gctcgccttg gtgggctagg
     1681 gggagcattc cccgttcgcc gggaatcaag cggttcgacc taggaaccgc ccgccgggga
     1741 ctcggcgacg tggtcggcgg cgtgcccgcc gcgctccccc atgggctgct ccggcccctt
     1801 ggtcgtgtcc cgggccgtct gcagctccat ctcggtgttg agctcggccc cgaccagcac
     1861 gatcaccgcc gtcaggtaga gccagaacat caggatgatg accccggcca gcgccccgta
     1921 ggtcttgttg tagttgccga aggagttggc gtagatcgag aacccgatcg aggccacgat
     1981 ccacagcacg gtcgcgaccc ccgagcccca ggtcacccag gtccacctgg gctcgtcgcg
     2041 gtcgggggcg aaccggtaga gcgcggccag gccgacgatg aggagggccg ccagcagcag
     2101 gaagcgcagg atggagacca cccactgcac caccaccggc aggtcgttgg cgatcggggg
     2161 gaagccgacg atcgtggccc cggccaccgc cagcagcccc atcgcgccca gggtcatcac
     2221 gatggcgcgc ccgcgcagct tgacgaactt gcggctctcg ctctcgtcat aggccaggtt
     2281 gaccccggtg atcagcgcct tcatgccgct ggaggcgctc cacagggcgg tcaggatccc
     2341 gatgacggtg gcgacgccga gggcgccgcc ggcgccgctg gtgatctgct cgatctggtc
     2401 gccgacgagc ttggccgtct cgggggagag ccgctgggcc agccggtcga tctggtcgcg
     2461 gacggtctgg gggtcggcca ccagcccgta gagggagacg ccggcgatga tggccgggaa
     2521 cagcgacagc aggatgtaga aggcgacccc ggccccgagc agcgggacct ggtcctgctt
     2581 gacctccttg gcggtccgct tggcgatgtc cttccagccc ttgggcggga tctccgtcgg
     2641 ggtgtcggcc tggcggccgt gctcgttgtt ggtgacggcg ccggggtcgg tctcg
//
LOCUS       DASSWR010000293         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_548505,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000293 DASSWR010000000
VERSION     DASSWR010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548505"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..327)
                     /locus_tag="VFO47_04935"
     CDS             complement(40..327)
                     /locus_tag="VFO47_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009067305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8788072.1"
                     /translation="MQRRTGEPYLEFLRRDSMSCGLYVLEAGADDPQEPHQEDEVYLV
                     LAGRARLDVAGRGHPVGPGSVLFVARTVPHRFHDVTERLSVLVIFAPAETV"
     gene            432..1400
                     /locus_tag="VFO47_04940"
     CDS             432..1400
                     /locus_tag="VFO47_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8788073.1"
                     /translation="MRICRYVADGGTARVGYLDGELIVPVAGGDGQAGLDAVLDLAMA
                     ANADSDLRPRPDGDPVPLASARLLAPVTSPPSIRDFYAFEQHVRTARSRRGKEMHPDW
                     YELPIFYFTNPAAVLGPGDPVAAPPRSAELDYELEVACVLGRGGANLRLDDADATVAG
                     FMVMNDWSARDVQRREMALSLGPAKGKDFATSLGPTLVTTAEFAPGGLREVPSAVMTA
                     TVNGVEWSRADLDGLWWSFAEMLVYASEAAPVRRGDVLGSGTCGTGCILELSLVHGAD
                     KYPYLQPGDEVELEVAGLGVLANRVVAGDAPAFQPDPDRMRPRLGQ"
     gene            1397..1888
                     /locus_tag="VFO47_04945"
     CDS             1397..1888
                     /locus_tag="VFO47_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET8788074.1"
                     /translation="MSARGPGAGTDRAGRLLAAAGRIRASAVVLPDGALTDPDPESGE
                     RWDRGQVLAHVAEMLPYWAQQAELIAAGRQTEFGRVSSDPDRIAAIERDRREDPQRLL
                     GRVDEGVAVVLALLDRLDAQALARTGHHQSLGDMTVAEIVDRFAVAHLEEHADQLDPP
                     APG"
     gene            1949..2710
                     /locus_tag="VFO47_04950"
     CDS             1949..2710
                     /locus_tag="VFO47_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8788075.1"
                     /translation="MTEATFQAEVLERSKTVPVLVDFWADWCGPCHALAPVLERAVER
                     HGGEVVLARVDVDQNQRLAMAFQVQGIPAVKAFAGGRLVDQFVGAQPAQVVERFVESL
                     LPSDADRAAAAAADLDPAQAAARWREVLEEDPDNVAARAGLADLALREGRPAEAIEVL
                     GPVEHDPEVAVLLASARLAAEAADPGSCFAAAAGQAADGQADPALATLLAAVREGPGE
                     TRDKARGLMLDVFRLLGDGHPLTARYRKDLMRALF"
     gene            2756..>2929
                     /locus_tag="VFO47_04955"
     CDS             2756..>2929
                     /locus_tag="VFO47_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET8788076.1"
                     /translation="MAKEAVLTPQGEDFPQWYQDVVAKAEMAESGAARGTMVIRPYGY
                     GLWERMQSEMDDRI"
BASE COUNT          376 a         1101 c         1087 g          365 t
ORIGIN      
        1 accgtcgcga tccacgacgg cccggatgaa ggcctccatc tagaccgtct cggccggggc
       61 gaagatgacc agcaccgaga ggcgctcggt gacgtcgtgg aagcggtggg ggacggtgcg
      121 ggcgacgaac agcacgctgc ccggccccac cgggtggccg cggccggcga cgtccagccg
      181 ggcccggccg gccaggacca ggtagacctc gtcctcctgg tgaggttcct gggggtcgtc
      241 ggctccggcc tcgagcacgt acagcccgca gctcatcgag tcgcggcgca ggaactcgag
      301 gtacggctcg ccggtgcggc gctgcacctc ggcccgcgcc tcctcgaggg aggggacgtc
      361 gaaggcctcg gcagggggct cgtccaccag gcaatcctaa cctggccggc gggcgcggta
      421 tggtgagcgc catgaggatc tgccgctatg tggccgacgg cgggacggcc cgggtgggct
      481 acctggacgg ggagctgatc gtgcccgtgg ccggcggcga cggccaggcc gggctggacg
      541 ccgtgctcga cctggccatg gccgccaacg ccgactcgga cctgcggccc cggcccgacg
      601 gcgacccggt ccccctggcc tcggcccggc tgctcgcccc cgtgaccagc ccgccgtcga
      661 tccgcgactt ctacgccttc gagcagcacg tccgcaccgc ccggtcacgc cgcggcaagg
      721 agatgcaccc cgactggtac gagctgccga tcttctactt caccaacccg gcggccgtgc
      781 tcggccctgg cgacccggtg gccgcgccgc cccgctcggc cgagctcgac tacgagctcg
      841 aggtggcctg cgtgctcggc cgcggtggcg ccaacctgcg cctggacgac gccgacgcga
      901 cggtcgccgg gttcatggtc atgaacgact ggtcggcccg ggacgtgcag cggcgtgaga
      961 tggcgttgtc gctgggcccg gccaagggca aggacttcgc cacctcgctc ggcccgacgc
     1021 tggtcaccac ggccgagttc gcccccgggg ggctgcggga ggtgccgagc gcggtgatga
     1081 cggccacggt caacggggtc gagtggtcgc gggccgacct cgacgggctg tggtggagct
     1141 tcgccgagat gctggtctac gccagcgagg cggccccggt gcgccgtggc gacgtgctcg
     1201 ggtccggcac ttgcgggacc ggctgcatcc tggagctgtc gctggtccac ggggccgaca
     1261 agtaccccta cctccagccc ggcgacgagg tcgagctgga ggtggccggc ctgggcgtgc
     1321 tcgccaaccg ggtcgtggcc ggcgacgccc ccgccttcca gcccgacccc gaccgcatgc
     1381 gcccgaggct gggccagtga gcgcccgggg cccgggtgcc gggaccgatc gggccgggcg
     1441 gctgctggcc gcggccgggc ggatccgggc cagcgccgtg gtcctgcccg acggcgccct
     1501 gaccgacccc gacccggagt cgggggagcg ctgggaccgg ggccaggtcc tggcccacgt
     1561 ggccgagatg ctgccctact gggcccagca ggccgagctg atcgccgccg gccgccagac
     1621 cgagttcggc cgggtctcct ccgacccgga ccggatcgcg gccatcgagc gcgaccgccg
     1681 cgaggacccc cagcggctcc tcggccgggt cgacgagggc gtcgccgtgg tcctggccct
     1741 gctcgaccgg ctcgacgccc aggccctggc ccgcaccggc caccaccaga gcctcggcga
     1801 catgacggtg gccgagatcg tcgaccggtt cgccgtcgcc cacctggagg agcacgccga
     1861 ccagctcgac ccgcccgcgc cgggctgacg ttgcgggagg ggcgcaggca ggcgtaccta
     1921 caagggtatg agcgatctag tctatgacgt gaccgaagcc accttccagg ccgaggtgct
     1981 ggaacggtcc aagaccgtgc ccgtgctggt cgacttctgg gccgactggt gcggcccctg
     2041 ccatgccctg gcgccggtgc tggagcgggc cgtcgagcgc cacggcggcg aggtcgtcct
     2101 ggcccgggtg gacgtcgacc agaaccagcg cctggccatg gccttccagg tccagggcat
     2161 cccggcggtc aaggcgttcg ccggcggccg cctggtcgac cagttcgtcg gcgcccagcc
     2221 ggcgcaggtg gtcgagcggt tcgtcgagtc cctgctgccc agcgacgccg accgggccgc
     2281 cgcggccgcg gccgacctcg accccgccca ggccgccgcc cgctggcggg aggtcctgga
     2341 ggaggacccc gacaacgtcg ccgcccgggc cggcctggcc gacctggccc tgcgcgaggg
     2401 ccgcccggcc gaggcgatcg aagtcctcgg cccggtggag cacgaccccg aggtcgccgt
     2461 gctgctggcc agcgcccgcc tggccgccga ggccgccgat cccggctcct gtttcgccgc
     2521 cgccgccggc caggccgccg acggccaggc cgacccggcc ctggccaccc tcctggccgc
     2581 cgtccgcgag ggcccgggcg agacccgcga caaggcccgc gggctgatgc tggacgtctt
     2641 ccgcctcctg ggcgacggcc acccgctcac cgcccgatat cgcaaggacc tgatgagggc
     2701 gttgttctag actgcctccc gaacccctcg aaacgagaag gaggcgggac ccatcgtggc
     2761 caaggaggcc gtgctcaccc ctcagggtga ggacttcccc cagtggtacc aggacgtcgt
     2821 cgccaaggcg gagatggccg agtcgggcgc cgcccgcggc acgatggtga tccgccccta
     2881 cggctacggc ctgtgggagc ggatgcagtc ggagatggac gaccgcatc
//
LOCUS       DASSWR010000294         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1282939, whole genome shotgun sequence.
ACCESSION   DASSWR010000294 DASSWR010000000
VERSION     DASSWR010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282939"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFO47_04960"
     CDS             complement(<1..413)
                     /locus_tag="VFO47_04960"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788077.1"
                     /translation="MAAVSARTFRPSAGQRRLAAKLLGAVLSLAFVLVFNFFLFRVLP
                     GDPAKNLTRNRLVPAEQVEVLRESFGLGKPLPEQFASYVRDTLQGDLGISYKFRRPVS
                     EVIAERIWPTVLLLGLSTLLSTAIGLWIGIRGAWRR"
     gene            complement(426..2219)
                     /locus_tag="VFO47_04965"
     CDS             complement(426..2219)
                     /locus_tag="VFO47_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8788078.1"
                     /translation="MVVLAALLALTAAPGVTAQEQPAGKLTFTVGIINDVDSLNPFIG
                     ILAETYEVWALMYDYLVGYSQKDFSPVPGLAESWEVSDDELTWTYKIRQGVKWSDGQP
                     LTAKDAAYTFNRIMKGTFEQTNYGNYVSNIRTVEAPDDATLVMTTKEPSPTMLRLAVP
                     ILPEHVWKDIDEKEVSTFDNEKNAVGSGPFVLAERSTGQFVRLTANKEYWDGAPKIDE
                     VVYRVFNNADAQLQALKKGEIDFADGLDPAPFNSLKDTEGITAVAGDYSGFDELAFNT
                     GAALDSGELIGDGHPALKDKRVRQAIAHAVDKQALVDRVLGGYGTPAAGVIPALYQNL
                     TFQPADGEAYNFDLAEANRLLDEAGYKDSDGDKVREMPDGSRPLRFRLFARQESNTSQ
                     QSVQFMQGWLRDIGIATEVKVVEENRLTEIIGQGEFDMFEWGWVVEPDPDYQLSTFTC
                     GSRSYKSGGDVLANLSDSFYCNTEYDKLYEQQKVTIDPAKRAEIVKQMQRMLYVDAPY
                     VVTFYYDELQAYRSDRFAGFLAQPDPGGVVLFQYGTYSYRNIATPQATAAAGDEGGGV
                     PLVPIAVGVAVVGAAGVLLALRRRSTQDERE"
BASE COUNT          345 a          813 c          852 g          411 t
ORIGIN      
        1 cgccgccacg ccccccggat gccgatccac agcccgatgg ccgtcgacag cagcgtggac
       61 agccccagca gcagcaccgt cggccagatg cgctcggcga tcacctccga gaccggccgg
      121 cggaacttgt aggagatgcc gaggtcgccc tggagggtgt cgcggacgta gctggcgaac
      181 tgctcgggca gcggcttgcc cagcccgaag ctctcccgca ggacctcgac ctgctcggcc
      241 gggaccaggc ggttgcgggt caggttcttg gccgggtccc cgggcaggac ccggaacagg
      301 aagaagttga acaccagcac gaaggccagg ctgagcacgg cccccaggag cttggcggcg
      361 agccggcgct ggccggcgct tggccggaag gtccgggcgg agaccgcggc catcgggccg
      421 gtgccctact cgcgctcgtc ctgggtcgat ctccgccgca gcgccagcag caccccggcc
      481 gccccgacca cggccacacc gacggcgatg ggcacgagcg gcacgccgcc gccctcgtcg
      541 ccggccgcgg cggtcgcctg cggcgtggcg atgttgcggt agctgtaggt gccgtactgg
      601 aacaggacca cgccgcccgg gtcgggctgg gccaggaagc cggcgaagcg gtcgctgcgg
      661 taggcctgga gctcgtcgta gtagaaggtc accacgtacg gcgcgtccac gtagagcatc
      721 cgctgcatct gcttgacgat ctcggcccgc ttggccgggt cgatggtcac cttctgctgc
      781 tcgtacagct tgtcgtactc ggtgttgcag tagaaggagt cggacaggtt ggccaggacg
      841 tcgccgcccg acttgtagct gcgcgagccg caggtgaagg tcgagagctg gtagtcgggg
      901 tcgggctcga ccacccagcc ccactcgaac atgtcgaact cgccctggcc gatgatctcg
      961 gtcaggcggt tctcctccac caccttgacc tcggtggcga tgccgatgtc gcgcagccag
     1021 ccctgcatga actggaccga ctgctgggag gtgttggact cctgccgggc gaacagccgg
     1081 aacctgagcg gccggctgcc gtcgggcatc tcccggacct tgtcgccgtc ggagtccttg
     1141 tagccggcct cgtcgagcag ccggttggcc tcggccaggt cgaagttgta ggcctcgccg
     1201 tcggccggct ggaaggtcag gttctggtag agggccggga tgaccccggc ggccggggtg
     1261 ccgtagccgc ccagcacccg gtcgaccagc gcctgcttgt cgacggcgtg ggcgatcgcc
     1321 tggcggaccc gcttgtcctt gagggccggg tggccgtcgc cgatcagctc gccgctgtca
     1381 agggccgccc cggtgttgaa ggccagctcg tcgaagccgg agtagtcgcc ggccacggcg
     1441 gtgatgccct cggtgtcctt gagggagttg aacggcgccg ggtcgagccc gtcggcgaag
     1501 tcgatctcgc cctttttgag ggcctggagc tgggcgtcgg cgttgttgaa gacccggtac
     1561 acgacctcgt cgatcttggg cgcgccgtcc cagtactcct tgttggccgt caggcgcacg
     1621 aactggccgg tgctgcgctc ggcgagcacg aacgggcccg agccgacggc gttcttctcg
     1681 ttgtcgaagg tgctgacctc tttctcgtcg atgtccttcc agacgtgctc gggaaggatc
     1741 ggcacggcca gccgcagcat ggtcgggctg ggctccttgg tggtcatcac cagggtggcg
     1801 tcgtcggggg cctcgacggt cctgatgttg gagacgtagt tgccgtagtt ggtctgctcg
     1861 aaggtgccct tcatgatccg gttgaaggtg taggcggcgt ccttggccgt gagcggctgg
     1921 ccgtcggacc acttcacccc ctggcggatc ttgtaggtcc aggtcagctc gtcgtcggag
     1981 acttcccagg actcggccag gccgggcacg ggcgagaagt ccttctggct gtagccgacc
     2041 aggtagtcgt acatcagcgc ccagacctcg taggtctcgg ccaggatgcc gatgaacggg
     2101 ttgagcgagt cgacgtcgtt gatgatgccg acggtgaagg tgagcttgcc ggccggctgc
     2161 tcctgggcgg tcactcccgg cgccgccgtc agggccagca gggccgccag caccaccatc
     2221 accgaggtcc tccggaaccg tttcgttccc cggttccacc ggggggccag gtctgagccc
     2281 cccggacccc cccggctccg cccgttcctg cctgcaccgc tgaatgcgcc cacgcccggc
     2341 ccccttgcgg gattcccacc accgccgaca aagctgactg aacagtcagt aattaggagt
     2401 tgcgctattg tgcgatgccg c
//
LOCUS       DASSWR010000295         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_802484,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000295 DASSWR010000000
VERSION     DASSWR010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802484"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1847
                     /locus_tag="VFO47_04970"
     CDS             195..1847
                     /locus_tag="VFO47_04970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HET8788079.1"
                     /translation="MVEGHGGTLAVAAARRHGVEELFTLSGGHVFPLYDGAVTADPPL
                     RLVDVRHEQTAVFAAEATAKLLRRPGLAVLTAGPGVTNGVSAVTTAHFNGSPVLVLGG
                     RAPTGRWGAGSLQELDHPPLVATVTKHAGTVADVGDVAGEVDRALRLAAEPHRGPVFL
                     DIALEALFSQGTAELPDPGPPAARDPDPDALDAVARLVGQAERPVLVLGSDVWADRAE
                     DAARRCAEALALPVVANGQGRGILAPGGDGLLVTRARSLAFGAADLVLVAGAPLDFRL
                     GYGTFGGRGGGPPAKVVHLVDTPGQVAGHVDLAASVAGDLGLVLDGLAGAVAALPGRR
                     PGTGPWLARLRDQARAAAAADRELLDSDADPIHPARVYGELLRVLADDAVVIGDGGDF
                     VSFAGRFVEPARPGNWLDPGPYGCLGTGLGYAVAARLARPSAQVVLLLGDGAAGFSLM
                     DVDTLVRHRLPVVLVCGNNGIWGLEKHPMRMLYGYDVAADLQPQCRYDLVAEALGAAG
                     ELVTTPAQLGPALRRAFDAGVPSLVNVATDPEVTYPRSTSGL"
     gene            complement(1844..>2355)
                     /locus_tag="VFO47_04975"
     CDS             complement(1844..>2355)
                     /locus_tag="VFO47_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET8788080.1"
                     /translation="PCQASRAYGPSRGVPVQQPVAGRGDDVPRGPQPVGAALGAAMVE
                     LLARSDPDARRLLGRVERPLARLWLAAWLPDAPRGTLVEPLCKIGPYRVDFLVLPRVV
                     VEIDGEGPLRASSDQVVRERRRDRYLVAQGYAVLRFAGDEVFANPWRCAEEVRDYLLG
                     RRVAGLEGA"
BASE COUNT          245 a          926 c          913 g          271 t
ORIGIN      
        1 gaccagcggg cgcggcctgg agcggttcat ggctggccac gatgccagag gcccgcgccg
       61 tccgcccagc cggcgggacc ggccaactcg gcgaggtgcg ggcgagccgg atagcatcgg
      121 gcgggcccgg ggggggtccg ggggaccgcc ggggtggtcc cccggtgaat cggcgggagg
      181 aacggttggg cgggatggtc gaggggcacg ggggcacgct ggccgtggcg gcggcccggc
      241 ggcacggggt cgaggagctg ttcaccctgt ccggcgggca cgtgttcccc ctgtacgacg
      301 gggcggtcac ggccgacccg ccgctgcggc tggtcgacgt ccgccacgag cagacggcgg
      361 tgttcgcggc cgaggccacg gccaagctgc tgcgccggcc agggctggcc gtgctcaccg
      421 ccgggcccgg cgtgaccaac ggggtcagcg ccgtcaccac cgcccacttc aacggctcgc
      481 cggtgctggt gctggggggc cgggccccga ccggccgctg gggcgccggc agcctccagg
      541 agctcgacca cccgccgctg gtcgccaccg tcaccaagca cgccggcacc gtcgccgacg
      601 tcggggacgt cgccggcgag gtcgaccggg cgctgcgcct ggccgccgag ccgcaccggg
      661 gcccggtgtt cctcgacatc gccctggagg cgctgttctc ccaggggacg gccgagctgc
      721 ccgaccccgg cccgccggcc gcccgcgacc ccgacccgga cgcccttgac gcggtggccc
      781 ggctggtcgg ccaggccgag cggccggtac tggtgctggg gtccgacgtc tgggccgacc
      841 gggccgagga cgcggcccgc cgctgcgccg aggccctggc cctgccggtg gtggccaacg
      901 gccagggccg cggcatcctc gcccccggcg gcgacggcct gctggtgacc agggcccgct
      961 cgctcgcctt cggcgccgcc gacctggtgc tggtggccgg cgccccgctc gacttccggc
     1021 tcggctacgg caccttcggc ggccgcggcg gcggcccccc ggccaaggtc gtccacctgg
     1081 tcgacacccc cggccaggtg gccggccacg tggacctggc cgcgagcgtg gccggcgacc
     1141 tcggcctggt cctcgacggc ctggccgggg cggtggccgc cctgcccggc cggcgccccg
     1201 gcaccgggcc gtggttggcg cggctgcgcg accaggcccg ggccgcggcg gccgccgacc
     1261 gggagctgct ggacagcgac gccgacccga tccacccggc ccgggtctac ggggagctgc
     1321 tccgcgtcct ggccgacgac gccgtggtga tcggcgacgg cggcgacttc gtctccttcg
     1381 ccggccggtt cgtggagccg gcccggccgg gcaactggct cgaccccggg ccctacggct
     1441 gcctcggcac cggcctcggc tacgccgtcg ccgcccgcct ggcccgcccc tcggcccagg
     1501 tcgtgctcct gctgggcgac ggcgcggccg ggttctccct gatggacgtg gacaccctgg
     1561 tccgccaccg cctcccggtg gtgctggtct gcggcaacaa cggcatctgg ggcctggaga
     1621 agcatcccat gcggatgctg tacggctacg acgtggccgc cgacctccag ccccagtgcc
     1681 gctacgacct ggtcgccgag gccctcggcg ccgccggcga gctggtcacc accccggccc
     1741 agctcggccc ggccctgcgc cgcgccttcg acgccggcgt cccctccctg gtcaacgtgg
     1801 ccaccgaccc cgaggtcacc tacccccgct ccacctccgg gctctaggcc ccctccaggc
     1861 cggccacgcg gcggccgagg aggtagtcgc ggacctcctc ggcgcagcgc caggggttgg
     1921 cgaagacctc gtcgccggcg aagcggagga cggcgtagcc ctgggcgacc aggtagcggt
     1981 cgcggcggcg ctcgcggacg acctggtcgg agctggcccg gagcgggccc tcgccgtcga
     2041 tctcgaccac cacccggggc aggaccagga agtcgacccg gtaggggccg atcttgcaga
     2101 ggggctcgac cagggtgccc ctgggggcgt cggggagcca ggcggccagc cacaggcggg
     2161 ccagggggcg ctccacccgg ccgaggaggc gccgggcgtc ggggtccgac cgggccagca
     2221 gctcgaccat ggcggcgccg agggcggccc cgaccggctg cggccctcgt ggtacgtcgt
     2281 cgccgcgtcc ggcgaccggc tgctgcactg gcactcccct gctcggcccg tacgcgcgtg
     2341 aggcttggca aggta
//
LOCUS       DASSWR010000296         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1197348, whole genome shotgun sequence.
ACCESSION   DASSWR010000296 DASSWR010000000
VERSION     DASSWR010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197348"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1100
                     /locus_tag="VFO47_04980"
     CDS             63..1100
                     /locus_tag="VFO47_04980"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET8788081.1"
                     /translation="MSSLPIPDFTAPRQVLLAAPRGWCAGVERAVDIVEVALATYGPP
                     VYVRKQIVHNLHVVRDLETRGAVFVDELDEVPEGATVVFSAHGVAPSVHAEARTRGLR
                     VVDATCPLVTKVHQEARKFAAKGYDIVLIGHEGHEEVVGTMGHAPRAVHLVGDEDEAS
                     RVEVADPERVAYLTQTTLSVDETAGMIDSLRQRFPVLAGPRSDDICYATQNRQAAVKT
                     LARDCQLVLVVGATNSSNSVRLVEVARAAGAQAHLVPEASAVDPAWFEGVDTVGVSSG
                     ASAPEWLVDELVEALRERGATEVREVEVVPEDVHFALPGTLRRDAEAARAGGVRGTAG
                     VVPRSVGRPGG"
     gene            1101..2357
                     /locus_tag="VFO47_04985"
     CDS             1101..2357
                     /locus_tag="VFO47_04985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8788082.1"
                     /translation="MAGRDGGVYVVGAGLAGLRCARRLHDKGVAATVLEASDGVGGRV
                     RTDRVEDFLLDRGFQVLLTAYPEARDALDYRELELHPFYPGAMVHTGDRFVTVADPFR
                     RRWDGLRTALAPVGGLGDKLKVASLRRRVTAGALEELFARPETTTSEALAADGLSDAI
                     VDRFFRPLFGGILLDRELGTSSRMFEFVYRMFSIGDVALPTRGMGAIPEQLAGGLPPD
                     CVRLGQRVTAVGDGAVTLDGGEKLPARAVVVATDGPTASQLLGDDLPAPESVAATCLY
                     FAADRAPVDEPVIVLDGDGQGPVNNLCVPSAVAPTYAPPGAALISAAVIDRPGLPKGP
                     DLEAAVLGQLADWFGSGVVAWRHLKTYHIPHAQPAQPPGTLDPPERSVRLRPGLYVCG
                     DHRDTASINGALASGRRTAEVVLEDL"
BASE COUNT          298 a          988 c          857 g          314 t
ORIGIN      
        1 gttgtccaca aggtacgccc gcccgccggg cgcgagcggc cggcgccgat agaatggggg
       61 acatgagcag cctccccatc ccggacttca ccgccccccg ccaggtgctg ctggccgccc
      121 cccgcggctg gtgcgccggc gtcgagcggg ccgtcgacat cgtcgaggtc gccctggcca
      181 cctacggccc cccggtctac gtgcgcaagc agatcgtcca caacctgcac gtggtccggg
      241 acctcgagac caggggcgcg gtgttcgtcg acgagctcga cgaggtgccc gagggggcga
      301 cggtggtgtt ctcggcccac ggcgtcgccc cgtcggtcca cgccgaggcc aggaccaggg
      361 gcttgcgggt ggtcgacgcc acctgcccgc tggtgaccaa ggtccaccag gaggcgcgca
      421 agttcgccgc caagggctac gacatcgtgc tcatcggcca cgagggccac gaggaggtcg
      481 tcggcaccat gggccacgcg ccccgggccg tccacctcgt cggcgacgag gacgaggcgt
      541 ccagggtcga ggtcgccgac cccgagcggg tcgcctacct cacccagacc accctgtcgg
      601 tggacgagac cgccgggatg atcgacagcc tgcgccagcg gttcccggtg ctggccggcc
      661 cccgctccga cgacatctgc tacgccaccc agaaccggca ggcggcggtc aagacgctgg
      721 cccgcgactg ccagctggtc ctggtcgtcg gggccaccaa ctcctccaac tcggtccgcc
      781 tggtcgaggt cgcccgggcc gccggcgccc aggcccacct ggtcccggag gcgtcggcgg
      841 tcgacccggc ctggttcgag ggcgtcgaca cggtcggggt cagctccggg gcctccgccc
      901 ccgagtggct ggtcgacgag ctggtcgagg ccctgcgcga gcgcggcgcg accgaggtgc
      961 gcgaggtcga ggtcgtcccc gaggacgtcc acttcgccct gccggggacg ctgcgccggg
     1021 acgccgaggc ggcccgggcc gggggggtcc gggggaccgc cggggtggtc ccccggtcgg
     1081 tcggacggcc tggcggttag atggccgggc gggacggcgg cgtctacgtc gtcggcgccg
     1141 ggctggccgg gctccgctgc gcccgccgcc tccacgacaa gggcgtggcc gccaccgtgc
     1201 tggaggcctc cgacggggtc ggcggccggg tccgcaccga ccgggtcgag gacttcctgc
     1261 tcgaccgggg cttccaggtc ctgctgaccg cctaccccga ggccagggac gccctcgact
     1321 accgcgagct ggagctgcac cccttctacc cgggggcgat ggtccacacc ggcgaccggt
     1381 tcgtcaccgt ggccgacccg ttccgccgcc gctgggacgg gctgcgcacc gccctggccc
     1441 cggtgggcgg cctcggcgac aagctcaagg tggcgtcgct gcgccgacgg gtcaccgccg
     1501 gggccctgga ggagctgttc gcccggcccg agaccacgac cagcgaggcc ctggcggccg
     1561 acggcctgtc ggacgcgatc gtcgaccgct tcttccggcc gctgttcggc gggatcctgc
     1621 tcgaccggga gctgggcacc tccagccgca tgttcgagtt cgtctaccgg atgttctcca
     1681 tcggcgacgt cgccctcccg acccgcggca tgggcgccat ccccgagcag ctcgccggcg
     1741 gcctgcctcc cgactgcgtc cgcctcggcc agcgggtcac ggccgtcggc gacggcgccg
     1801 tcaccctcga cggcggcgag aagctgccgg ccagggcggt cgtggtcgcc accgacggcc
     1861 cgaccgcgtc ccagctgctc ggcgacgacc tgccggcccc ggagtcggtc gcggccacct
     1921 gcctctactt cgccgccgac cgggccccgg tcgacgagcc ggtgatcgtc ctcgacggcg
     1981 acggccaggg cccggtcaac aacctctgcg tccccagcgc cgtcgccccc acctacgccc
     2041 cacccggcgc cgccctcatc tcggccgccg tcatcgaccg ccccggcctc cccaagggcc
     2101 ccgacctgga ggccgccgtc ctcggccagc tcgccgactg gttcggctcc ggcgtggtcg
     2161 cctggcgcca cctcaagacc taccacatcc cccacgccca gcccgcccag ccccccggta
     2221 ccctcgaccc ccccgaacgc tccgtccgcc tccgccccgg cctctacgtc tgcggcgacc
     2281 accgcgacac cgcctccatc aacggcgccc tcgcctccgg ccgccgcacc gccgaggtgg
     2341 tcctggagga cctctgaccc ctcccccggt gtcgggacgg cgaggtaggg ttggcgcatg
     2401 aacgagcaca tcgatgtcca ggccggggaa gtcctgctga ccgtcggcac catgaag
//
LOCUS       DASSWR010000297         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_802509,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000297 DASSWR010000000
VERSION     DASSWR010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802509"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="VFO47_04990"
     CDS             complement(<1..2160)
                     /locus_tag="VFO47_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET8788083.1"
                     /translation="MADLGPSRSGGETPSVTEPLTGEELNRLWADPPGLRGLLTGVQN
                     DKIGTRLLLTGFFFLLLGGSFDSFVMRMQLAVPENSLVSPQLYNELFTNHGTVVMFLV
                     ILPIFEGFAILLLPLLLGTREMPFPRLGAFSYYTFLLGGLLYYSSTLFRAVPDAGWFA
                     YTPLSGLEFSPDLALDFWVLGLGVAEVAAIAAGIEIIIGVLKMRAPGMTLSRIPLFAW
                     AVLVMAFMILFAFTTLIIASLLLELDRGFGTQFFNPQRGGSSLLWQHLFWIFGHPEVY
                     IQFVPATGVVSMIVATFARRRIVGYTWQVGALVAIGFLSFSLWAHHMFAVGLPTLVLS
                     FFAAASMVIALPAGVQFVSWLATIWSGRPVWKTPFLFVVGFLVTFVIGGITGVMVAAP
                     PFDLQAHDSYFVVGHFHYVLIGGVAFPIFAGVYYWFPKFTGRLLDERLGRWNFWLLFT
                     GVNLAFFPMHIVGLLGMPRRVYTYEAGLGWEPYNLLSSIGVLVIIMGIAVFIWNVARS
                     YRRGDEAGNNPWGAATLEWAVPSPPAEHGWSILPIVRSRHPLWDQDELHRGDERLERF
                     VHGLSRWPLRWRAAVIVGTADARPQEVFRVANPSIWPLVAGCGLVFIFAAELTKLRWG
                     IAAGAAIVTAAVIAWNWPQEAPMTVEEEAAFEAEHGVPVNAGGSVVVAAWGTALVILV
                     VAVAFSALLLSYFYLRLENPQWPPPGIADPGVGRVLLA"
     gene            complement(2153..>2461)
                     /locus_tag="VFO47_04995"
     CDS             complement(2153..>2461)
                     /locus_tag="VFO47_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET8788084.1"
                     /translation="WEVRYPEQGVTVRDELHLPVGRRVNLQLTSADVIHSFWVPELAG
                     KLDLLPDRTNTLVLEADQPGVHTSRCAEFCGLEHTRMVLTVVAESEERFAAWVAERRG
                     "
BASE COUNT          459 a          831 c          874 g          297 t
ORIGIN      
        1 ggccaggagg acccggccga caccagggtc ggcgatgccg ggtggtggcc actgggggtt
       61 ctccagccgc aggtagaagt agctgagcag cagggccgag aaggcgacgg cgacgaccag
      121 gatgaccagc gccgtccccc aggcggcgac gaccacgctg ccgccggcgt tgaccggcac
      181 gccgtgctcg gcctcgaagg cggcctcctc ctcgaccgtc atcggcgctt cctgcggcca
      241 gttccaggcg atcacggcag cggtgacgat ggccgcgccg gcggcgatgc cccaccggag
      301 cttggtcagc tcggcggcga agatgaacac cagcccacac ccggccacca gcggccagat
      361 cgacgggttg gcgacccgga acacctcctg gggtcgcgcg tcggcggtgc cgacgatcac
      421 cgcggcccgc cagcgcagcg gccaccgtga caggccgtgg acgaaccgct ccaggcgctc
      481 gtcgccccgg tggagctcgt cctggtccca cagggggtgg cggctgcgca cgatcggcag
      541 gatcgaccag ccgtgctcgg ccggcggcga cggcaccgcc cactccaggg tggcggcgcc
      601 ccaggggttg ttgcccgcct cgtcgccccg ccggtagctg cgggccacgt tccagatgaa
      661 cacggcgatg cccatgatga tgacgagcac cccgatgctg gagagcaggt tgtagggctc
      721 ccagcccagg ccggcctcgt aggtgtagac gcgcctgggc atccccagca gcccgacgat
      781 gtgcatcggg aagaaggcca ggttgacgcc ggtgaacagc agccagaagt tccaccggcc
      841 aaggcgctcg tccagcagcc ggccggtgaa cttcgggaac cagtagtaga cgccggcgaa
      901 gatcgggaac gcgaccccgc cgatcagcac gtagtggaag tgcccgacga cgaagtagga
      961 gtcgtgggct tggaggtcga agggtggggc ggccaccatc acgccggtga tcccgccgat
     1021 gacgaaggtc accaggaagc cgaccacgaa caggaacggc gtcttccaca ccggccggcc
     1081 cgaccagatg gtggccagcc aggagacgaa ctgcaccccg gcgggcaagg caatgaccat
     1141 gctggccgcg gcgaagaagc tcagcaccag cgtcggcagc cccacggcga acatgtggtg
     1201 ggcccacagg ctgaacgaca ggaacccgat ggcgaccagg gcgccgacct gccaggtgta
     1261 gccgacgatc cgccgccggg cgaaggtggc cacgatcatc gagaccaccc cggtggcggg
     1321 cacgaactgg atgtagacct cggggtggcc gaagatccag aacaggtgct gccacagcag
     1381 cgagctgccg ccccgctggg ggttgaagaa ctgggtgccg aagccccggt ccagctccaa
     1441 gagcaggctg gcgatgatga gggtggtgaa ggcgaacagg atcatgaacg ccatcaccag
     1501 caccgcccag gcgaacagcg ggatgcggct gagggtcatc cccggtgccc gcatcttcag
     1561 caccccgatg atgatctcga tgccggcggc gatcgccgcg acctcggcca cccccaaccc
     1621 caggacccag aagtccaggg ccaggtcggg tgagaactcc aggccggaca gcggggtgta
     1681 ggcgaaccag ccggcgtcag ggaccgcccg gaacagggtg ctgctgtagt acagcagccc
     1741 gccgagcagg aaggtgtagt aggagaaggc gcccagtcgg gggaacggca tctcgcgggt
     1801 gcccagcagc aggggcagca gcaggatggc gaagccctcg aagatcggca ggatcaccag
     1861 gaacatcacc acggtgccgt ggttggtgaa cagctcgttg tacagctgcg ggctcaccag
     1921 gctgttctcg ggcacggcca gctgcatgcg catcacgaag gagtcgaagc tgccccccag
     1981 caggaggaag aagaagccgg tgagcagcag ccgggtgccg atcttgtcgt tctggacccc
     2041 ggtcagcagc ccgcgcaggc cgggcgggtc ggcccacagc cggttgagct cctcgcccgt
     2101 cagcggctcg gtgaccgagg gcgtctcccc gcccgatcgc gaagggccca ggtcagccac
     2161 ggcgctcagc cacccaggcg gcgaaccgct cctctgattc ggcaaccacg gtcagcacca
     2221 tcctggtgtg ctccaggccg cagaactcgg cgcagcggct ggtgtggacg cccggctggt
     2281 cagcctccag gaccagggtg ttggtgcggt cgggcagcag atcgagcttg ccggccagct
     2341 ccggcaccca gaagctgtgg atcacgtccg ccgaggtgag ctgcaggttg acgcgacggc
     2401 ccaccggcag atggagctca tcccgcaccg tgaccccctg ctcggggtag cggacctccc
     2461 a
//
LOCUS       DASSWR010000298         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1394883, whole genome shotgun sequence.
ACCESSION   DASSWR010000298 DASSWR010000000
VERSION     DASSWR010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394883"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2202
                     /locus_tag="VFO47_05000"
     CDS             <1..>2202
                     /locus_tag="VFO47_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788085.1"
                     /translation="ADRLAADPDPVSWRQGRALPYGDGPTFGALAEVVKAEAGILESD
                     GAELAGRRLAVAAGAVADPVTAAWVTGHLRRLVGVGSRPGSAAGPTTAADRDEEFAAW
                     RRFLHGLAATRPLVLALEDLHRADDALLDFVEGLVGPGAAPAAVLVLATARPELLERR
                     PGWGAGGTTIRLGPLGDTDTTGLLATLLAHHGLPTEVDPALLARVGGNPLFAEEYVRM
                     LRDRADGSNPVPTGEPSGRSIHRGTPKGFPGPLRPDPLPTGVHAIVAARLDALPAPDK
                     AVLHDAAVLGQVGWLGALAEIAGRDLPELEAALGRLEAREFLQRAPASRVAGEVEYAF
                     RHTLVRDVAYGQVLRAERADKHRRAAAWIEGVAPDRAEGRAELLAYHYRAALSFARAA
                     GTEPPGLAGRALAALRDAGDRAATLGGWDTAARFHAEALELSPEGDPERGQLLLRLGR
                     ARCRGEMAGHDELTAGREALLAAGAPVAAAEAEMLLGELAFLQGRGQDREAHHERALA
                     LVAGTPPSPTKAAVLRGAMMHLVVASRHAEGLAVGREVLAMARTLGLPDLEADALGAI
                     GMARVEAGDPGGLDDLEAAIARFEKLGAPSGTLWHLNLGWAAATMGDLPRCFAALAAG
                     TRLAERFGSWRWRRSIELQRVAERYWTGRWTEVVAVVDDLLAGDEHHYLEWECRLWRG
                     RIHLAGGRLEQALADAEAAHALAVEARDPQDLHPTRAFLARTLLAAGRRAEA"
BASE COUNT          224 a          888 c          857 g          233 t
ORIGIN      
        1 ggccgaccgc ctggccgccg accccgaccc cgtgtcgtgg cggcagggcc gggcgctgcc
       61 ctacggcgac ggcccgacct tcggggcgct ggccgaggtg gtcaaggccg aggccggcat
      121 cctcgagagc gacggggccg agctcgccgg ccggcgcctg gccgtggccg cgggggcggt
      181 ggccgacccg gtcaccgccg cctgggtcac cggccacctg cgccgcctgg tcggggtcgg
      241 ctcccggccc ggatcggccg ccgggccgac caccgccgcc gaccgcgacg aggagttcgc
      301 cgcctggcgg cgcttcctgc acggcctggc cgccacccgg ccgctggtgc tggccctcga
      361 ggacctgcac cgggccgacg acgccctgct cgacttcgtg gagggcctgg tcggcccggg
      421 agccgccccg gcggccgtcc tggtcctggc cacggcccgg ccggagctgc tggagcgccg
      481 gcccggctgg ggcgccggcg ggaccaccat ccggctcggc cccctcggcg acaccgacac
      541 caccggcctg ctggccaccc tcctggccca ccacggcctg cccaccgagg tcgaccccgc
      601 cctgctggcc cgggtcggcg gcaacccgct gttcgccgag gagtacgtgc ggatgctgcg
      661 cgaccgcgcg gacggctcaa acccagtgcc gaccggggag ccgtcaggac gatcgatcca
      721 ccggggcacc ccgaaggggt tccccggacc cctccggccg gaccccctgc ccaccggcgt
      781 gcacgcgatc gtcgccgccc gcctggacgc gctccccgcc cccgacaagg cggtgctgca
      841 tgacgcggcc gtgctcggcc aggtcggctg gctgggcgcg ctggccgaga tcgccggccg
      901 cgacctcccc gagctggagg ccgccctcgg ccgcctggag gcccgcgagt tcctccagcg
      961 ggcgcccgcc tcgcgggtgg cgggcgaggt cgagtacgcc ttccgccaca ccctggtccg
     1021 cgacgtcgcc tatgggcagg tgctgcgggc cgagcgggcc gacaagcacc gccgggccgc
     1081 cgcctggatc gagggggtgg cccccgaccg ggccgagggg cgggccgagc tgctcgccta
     1141 ccactaccgg gccgccctgt cgttcgcccg cgccgccggg accgagccgc ccgggctggc
     1201 cggccgcgcc ctggccgccc tgcgggacgc cggcgaccgg gccgcgaccc tcggcggctg
     1261 ggacacggcc gcccgcttcc acgccgaggc cctggagctc agccccgagg gcgacccgga
     1321 acggggccag ctgctgctgc gcctgggccg ggcccgctgc cggggcgaga tggccggcca
     1381 cgacgagctc accgcgggcc gcgaggccct gctggccgcc ggcgcgcccg tggccgccgc
     1441 cgaggccgag atgctccttg gcgagctggc cttcctccag gggcggggcc aggaccgcga
     1501 ggcccaccac gaacgggctc tggccctggt cgccgggacg ccgccgtcgc cgaccaaggc
     1561 ggccgtgctc cggggcgcga tgatgcacct ggtggtggcc agccggcacg ccgagggcct
     1621 ggccgtcgga cgggaggtgc tggccatggc ccggacgctg ggcctgcccg acctggaggc
     1681 cgacgccctg ggggcgatcg gcatggcccg ggtcgaggcc ggcgacccgg gcgggctcga
     1741 cgacctggag gcggccatcg cccggttcga gaagctgggc gccccgagcg ggacgctctg
     1801 gcacctcaac ctcgggtggg cggccgccac catgggcgac ctgccccgct gcttcgccgc
     1861 cctggccgcc gggacgcggc tggccgagcg gttcgggtcc tggcgctggc ggcgctcgat
     1921 cgagctccag cgggtcgccg agcgctactg gaccgggcgc tggacggagg tcgtggccgt
     1981 cgtcgacgac ctgctcgccg gcgacgagca ccactacctg gagtgggagt gccggctgtg
     2041 gcggggccgg atccacctgg ccggcgggcg gctggagcag gccctggccg acgccgaggc
     2101 ggcccacgct ttggcggtgg aagcccgcga cccccaggac ctccacccga cccgggcctt
     2161 cctggccagg acgctgctgg ccgccggccg ccgggccgag gc
//
LOCUS       DASSWR010000299         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1254614, whole genome shotgun sequence.
ACCESSION   DASSWR010000299 DASSWR010000000
VERSION     DASSWR010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254614"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VFO47_05005"
     CDS             complement(<1..1198)
                     /locus_tag="VFO47_05005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8788086.1"
                     /translation="MSPAADRLVANRYALKDPLGRGGMGVVWRAQDSVLGREVAVKEV
                     VFPPTMAEEERGPAQARVMREARAAARLNHPGAVTLYDVVKDRGSTFIVMELVSAPTL
                     ADLVRTDGPLPVQRVAEIGAQVASALEAAHQAGIVHRDVKPGNVMVPANGTAKLADFG
                     IASLQGDPQLTSTGLVIGSPAYMAPEQAKGEESGPPADFWALGATMFYAVEGGPPFDR
                     GSSIATLAAVVNEPPRDLRRAGPLTPLITALLSKDPGARPSGPELRAELARLATPAPS
                     PPTEVLPTHGPGRTVPLAAFVPAPAGEGDGSGAVDSPEHPASGSATLPHRGTSQREGG
                     FPAAQREGVPAAGAPVPELAPALEPEPPAAEPPAAEAAGREPGRPLLPPAPVVGRGGR
                     GRLVAVL"
     gene            complement(1238..>2364)
                     /locus_tag="VFO47_05010"
     CDS             complement(1238..>2364)
                     /locus_tag="VFO47_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788087.1"
                     /translation="PADLWALGTILFLVVEGEPPFAADGSDATTAAILTDRPRQFVHA
                     GPLAPTLTALLTKPAGGRPGIAETRQLLQPLAGSPSASTLVEFPGAKPPARRGAPSPA
                     HPGAPAPADPGVRHPPGPVPGPPEGERSSWGGWGQAGVHSPLSRPARHSRAGAAMDPL
                     VRRVLLIAGGSVLLALVSFAVAVAVTGDPLGLRSQAVASTAATVPPPTLPPTTTSTPP
                     ITTPTTQAPLVPPGWSVHTDPATGYQVAVPPGWQLVSDGGPRTELRDQSTGAFLRIDR
                     VQDPQADPVTLEQQAGAAHAGERGGYQQARLEPAQFKGLPAALLEFTYQDGETWHALE
                     LGVRSPRHHVAMAIHTRDRDWGAGWALFEAFKASFVPPPT"
BASE COUNT          285 a          839 c          975 g          265 t
ORIGIN      
        1 cgagcaccgc aaccagccgg ccccgccccc cacgcccaac caccggcgcc ggcggcagca
       61 acggccgccc gggttcacgc ccggccgctt cggccgcggg tggttcggcc gcgggtggtt
      121 cgggctcgag cgccggcgcc agctccggca ccggcgcccc ggcagccggg acgccctccc
      181 gctgggctgc cgggaacccg ccctcccgct gcgaggtccc ccggtggggg agcgtagccg
      241 agccggaagc tgggtgctcc gggctgtcca cagccccaga cccgtcgccc tcccccgccg
      301 gggcggggac gaacgccgcc agcgggaccg tgcggcccgg gccgtgggtg gggaggacct
      361 cggtgggtgg ggacggggcg ggagtggcca ggcgggccag ctcggcccgg agctcggggc
      421 cggaggggcg ggcgccgggg tccttggaca ggagggcggt gatgaggggg gtgagcgggc
      481 cggcgcggcg gaggtcgcgg ggcggctcgt tgacgacggc ggccagggtg gcgatcgagc
      541 tgccgcggtc gaacggcggg ccgccctcga cggcatagaa catcgtcgcc ccgagggccc
      601 agaaatcggc cggggggccg ctctcctcgc ccttggcctg ctcgggggcc atgtaggcgg
      661 gcgagccgat gaccagcccg gtggaggtga gctgggggtc gccctggagc gaggcgatgc
      721 cgaagtcggc cagcttggcc gtgccgttgg cggggaccat gacgttgccg ggcttgacgt
      781 cgcggtggac gatcccggcc tggtgggccg cctcgagcgc gctggcgacc tgggcgccga
      841 tctcggccac ccgctggacc ggcagcggcc cgtcggtgcg gaccaggtcg gccagggtcg
      901 gcgcgctgac cagctccatc acgatgaagg tgctgccgcg gtccttgacc acgtcgtaca
      961 gcgtgaccgc ccccggatgg ttgagccggg ccgccgcccg cgcctcccgc atcaccctcg
     1021 cctgggccgg gccccgctcc tcctcggcca tggtgggcgg gaacaccacc tccttgacgg
     1081 ccacctcgcg acccagcacc gagtcctggg cacgccagac gacgcccatc cctccccgtc
     1141 ccaggggatc cttgagcgcg tagcggttgg cgaccagccg gtcggcggcg ggagacatac
     1201 cggaccaccc tacccgccga ccgccagtgc caagcgatca ggtgggcggc ggcacgaacg
     1261 acgccttgaa cgcctcgaac agggcccagc cggcgcccca gtcgcggtcg cgggtgtgga
     1321 tcgccatggc gacgtggtgc ctcggggagc ggacgcccag ctccagagcg tgccaggtct
     1381 cgccgtcctg gtaggtgaac tccagcaggg cggcgggcag gcccttgaac tgggccggct
     1441 ccagacgggc ctgctggtag ccgccgcgct cgccggcatg ggcggcaccg gcctgctgct
     1501 cgagggtcac cgggtcggcc tggggatcct ggacccggtc gatgcgcagg aacgccccgg
     1561 tgctctggtc gcgcagctcg gtgcgggggc cgccgtcgct gacgagctgc cagccgggag
     1621 ggacggcgac ctggtagccg gtggccgggt cggtgtggac ggaccagccg ggcgggacca
     1681 ggggcgcctg ggtggtcggg gtggtgatgg gcggggtcga cgtggtcgtc ggcgggaggg
     1741 tcggcggcgg gaccgtcgcg gccgtcgatg ccaccgcctg ggagcgcagg ccgagcgggt
     1801 cgccggtcac ggccacggcg accgcgaacg acacgagcgc gagcagcacc gacccacccg
     1861 cgatcagcag cacccgccgc accagcgggt ccatcgccgc cccggcccgc gagtggcggg
     1921 ccgggcggga cagggggctg tggacacccg cctgccccca accgccccac gagctacgct
     1981 ctccctccgg gggacctggc accggaccgg gcgggtggcg cacgcccgga tccgcgggag
     2041 ccggggcgcc ggggtgcgcc ggggacgggg cgccccgacg ggccgggggc ttcgcgcccg
     2101 ggaactcgac gagggtggac gcgctcgggc tccccgcgag aggttggagg agttggcggg
     2161 tctcggcgat gcccgggcga ccgccggcgg gcttggtcag gagggcggtg agggtggggg
     2221 cgagcggccc ggcgtgaacg aactgccggg gccggtcggt gaggatggcg gccgtggtgg
     2281 cgtcgctgcc gtcggctgcg aacgggggct caccctcaac tacaaggaac aggatcgtcc
     2341 ccagggccca gaggtcggct gggg
//
LOCUS       DASSWR010000300         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_350612,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000300 DASSWR010000000
VERSION     DASSWR010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350612"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFO47_05015"
     CDS             complement(<1..1127)
                     /locus_tag="VFO47_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET8788088.1"
                     /translation="MSLVLDDLPRDPDRLLHHLQQMAEVITQQNASLASLQDEHDAVL
                     AERDAAQAEIEKLRLLIRQLQRGQFGRRSERLDADQLQLGLEDLEQTAAAAEAAQEAA
                     ARNSTPRPPRVRRRNLGALPAHLPRVEVLVDVEDKSCPCCGGALHPIGEDTSEMLDLV
                     PAQLRVKVIRRPRYACRACEEAVVQAPAPERPVTGGMASEALLAHVLVAKYADFLPLY
                     RQAQIFARQGIALDRSTLCDWVGRACWWLEPLWRLLRRHVLGSTRIFADDTRLPVLDP
                     GRGRTKTGCLWGYAIDDRPWNGSTPPGVVYLYAEDRKGEHPAAHLAEFTGVLQVDGYG
                     GFKSLLDNRPSGAIRLAFCWAHCRRRFHELHQATGSPLAGEA"
     gene            complement(1173..1526)
                     /gene="tnpB"
                     /locus_tag="VFO47_05020"
     CDS             complement(1173..1526)
                     /gene="tnpB"
                     /locus_tag="VFO47_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET8788089.1"
                     /translation="MIAVPPGVRILLAARPVDFRKGMDGLAALVQQALRADPFAGEVF
                     VFRPKRADRVKILVYDGTGLCLCSKRLEAGRFSWPSPAEGMVRLSAAQLATLLEGLPW
                     HRLQPRPVRRPSTAW"
     gene            complement(1523..1885)
                     /locus_tag="VFO47_05025"
     CDS             complement(1523..1885)
                     /locus_tag="VFO47_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019200262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8788090.1"
                     /translation="MEVITGVGRRRRWSDEDKARIVAESLDPTTTSSAVARRYGLHAS
                     QLFAWRQQLQRSAAATAVLGEQEFVPVLLAEDGPAPAEPAGRMEIALGPVIVRVGADV
                     DAAALRRVLEVVRGLCLA"
BASE COUNT          313 a          751 c          669 g          289 t
ORIGIN      
        1 gcctccccgg ccaagggtga gccggtggcc tggtggagct cgtggaaccg tcttcggcaa
       61 tgcgcccaac agaaggccag ccggatcgcg cccgacgggc ggttgtccag caggctcttg
      121 aacccgccgt agccgtccac ctgcagcacg cccgtgaact cggccaagtg ggcggccggg
      181 tgctcgccct tgcgatcctc ggcatagaga tagaccaccc ccggcggggt gctgccgttc
      241 cagggtcggt catcaatcgc atagccccac aagcacccgg tcttggtccg gccccggccg
      301 gggtcgagca ccggcaagcg cgtgtcgtcg gcgaagatcc gggtcgagcc caggacatgc
      361 cggcgcaaca ggcgccacag cggctccagc caccagcagg cccggcctac ccagtcgcat
      421 aaggtcgatc ggtcgagcgc gatcccttgc cgggcgaaga tctgggcctg acggtagagc
      481 ggcaggaaat cggcatactt ggccaccagc acgtgcgcca ggagcgcctc gctcgccatc
      541 ccgccggtga ctggccgctc cggggccggg gcctgcacca ccgcctcctc gcaggcccgg
      601 caggcgtagc gcgggcggcg gatcaccttc acccgcagct gggcggggac gaggtcgagc
      661 atctcgctcg tgtcctcgcc gatcgggtgc aaggccccgc cgcagcaggg gcagctcttg
      721 tcctcgacgt cgaccaggac ctcgacccgc ggcaggtggg ccggcaaggc gccgaggttg
      781 cgtcgccgaa cccggggcgg ccgaggagtg ctgttccttg ccgccgcctc ctgcgccgct
      841 tcagccgccg cggcggtctg ctcgaggtct tccaggccaa gctgcagctg gtcagcgtcg
      901 agccgctccg agcgccggcc gaactgccca cgctgcagct gccggatcaa gagccgcagc
      961 ttctcgatct cggcctgcgc cgcgtcgcgc tcggccagaa cagcatcatg ctcgtcctgc
     1021 aatgatgcga gtgatgcatt ctgctgggtg atgacttcag ccatctgctg cagatgatgc
     1081 aacagtcggt caggatcgcg tggaagatca tccagcacca gcgacatggt gctattctat
     1141 tgtcggaaag ccgagaaggc caggacttcc gctcaccaag ccgtgctcgg ccgccgtacc
     1201 gggcggggtt ggaggcggtg ccacggcaag ccctcgagca gggtcgccag ctgcgccgcg
     1261 ctcaagcgca ccatcccttc cgccggcgag ggccagctga accgcccggc ctccagcctc
     1321 ttgctgcaca ggcacaggcc ggtcccgtca tagaccagga tcttcacccg gtcggcgcgc
     1381 ttgggacgga agacgaagac ctcgccggcg aacgggtcgg cccgtagcgc ctgttgcacc
     1441 agcgcggcca agccgtccat gcccttgcgg aagtcgaccg gccgggccgc gagcaggatg
     1501 cgcacccccg gcgggaccgc gatcacgcca ggcacaggcc ccgcaccacc tccagcaccc
     1561 ggcgcagcgc cgcggcgtcc acatcggcgc cgacgcggac gatgaccggg ccgagcgcga
     1621 tctccatccg gccggcgggc tcggccggtg ccgggccgtc ctccgccagc agcaccggca
     1681 cgaactcctg ctcgccgagc accgcagtcg ccgccgcgct ccgttgcagc tgctggcgcc
     1741 aggcgaacag ctggctcgcg tgcagcccgt agcgccgcgc caccgccgag gaggtcgtcg
     1801 tcgggtcgag gctctcggcc acgatccgcg ccttgtcctc gtccgaccag cggcgtcggc
     1861 gaccaacccc ggtgatgacc tccacccggc ggaagctgtc ggcgttcgta ctattcgtac
     1921 gaccatgatt acgcatagga gcaggcccgc tctgatcctc ccgggccctt catcctcatc
     1981 ctcgtcagcc ggcccgcaag gtggccttca aacgacgttt ac
//
LOCUS       DASSWR010000301         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_463344,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000301 DASSWR010000000
VERSION     DASSWR010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463344"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFO47_05030"
     CDS             complement(<1..887)
                     /locus_tag="VFO47_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET8788091.1"
                     /translation="MAPRLSLAPRADLGGLARAARAAGRLGIDTEFMSEGRYRALLCL
                     VGVVVDGPDGDGGPRVELVDPLDERLDPGPLAEVLADPGVEVVVHAGRQDVALLRRVW
                     QTEVRGIFDTQVAAGFAGFGAQTGYGSLLNDVLGLRLAKSASFTRWDDRPLSPEQLAY
                     ARDDVAHLLRLADALQERLQASGRLQWAREECRRLEEASDERDPWLAWQRLPRIGQLN
                     PRARAVARELAAWREQTAATEDRPVSSVLGDAPLVEVAKRRPGAPSALERIRGLQPST
                     LRRRADAIVAAVARGQAAEP"
     gene            complement(872..1912)
                     /locus_tag="VFO47_05035"
     CDS             complement(872..1912)
                     /locus_tag="VFO47_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET8788092.1"
                     /translation="MTAGNGHGNGAPVKLTAKDFKSDQEVRWCPGCGDYAILSAVQGF
                     MPSLGIPRERIVFISGIGCSSRFPYYMNTYGMHSIHGRAPAIATGLSVTRPDLSVWVI
                     TGDGDALSIGGNHLIHALRRNVNLKILLFNNQIYGLTKGQYSPTSELGKITKSTPMGS
                     LDHPFNPVSVALGAEATFVARSIDSDRAHLTEVLGKAAAHQGTAFVEIYQNCNIFNDG
                     AFDLLKEPDTRADNQIRLEHGKPIRFGADGERGVRLTDDCRIELVEVADVGEDALLVH
                     DEHRAEPSLAFMLCRLSHGADGPTPIGVFRDVERPVYDELMSEQLEAATTRQGRGDLA
                     KLLHGGDTWRLG"
     gene            complement(1909..>2686)
                     /locus_tag="VFO47_05040"
     CDS             complement(1909..>2686)
                     /locus_tag="VFO47_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HET8788093.1"
                     /translation="MFGRNGEAPVPIVAPRSPSDCFHAAIEAARIALKYRTPVFLLSD
                     GYLANGSEPWLLPDVDSLPDIGVEFATEPNHEGDYWPYLRDEATLARPWAVPGTPGLE
                     HRIGGLEKADGTGNISYDPDNHDRMVNLRAAKVAGMAADIAPLEVDHQDGSKLLVLGW
                     GSTYGPIGAAVRRVRASGARVSVAHLRHLNPFPANLGEVLAGYDKVLIPEMNLGQLRM
                     LVRSRFLVDAVGYNKVRGLPFKAEELADAIIGLLDEGATK"
BASE COUNT          349 a          960 c         1003 g          374 t
ORIGIN      
        1 ggctcggcgg cctggccgcg ggcgaccgcg gccacgatgg cgtcggcgcg gcggcgcagg
       61 gtcgagggct ggaggccgcg gatccgctca agagccgacg gcgcccccgg gcgccgcttg
      121 gccacctcga ccaggggggc gtcgcccagc accgaggaga cggggcggtc ctcggtggcg
      181 gcggtctgct cccgccaggc cgccagctcg cgggccacgg cccgggcccg cgggttgagc
      241 tggccgatgc ggggcaggcg ctgccaggcc agccacgggt cgcgctcgtc gctggcctcc
      301 tccaggcgcc ggcactcctc ccgtgcccac tggaggcgac cgctggcctg cagccgctcc
      361 tggagggcgt cggccaggcg cagcaggtgg gcgacgtcgt cgcgggcgta ggcgagctgc
      421 tcggggctga gcgggcggtc gtcccagcgg gtgaagctgg ccgacttggc caggcgcagg
      481 ccgagcacgt cgttgagcag gctgccgtac ccggtctggg ccccgaaccc ggcgaacccg
      541 gccgccacct gggtgtcgaa gatcccccgg acctcggtct gccagacccg ccggagcagg
      601 gccacgtcct ggcgcccggc gtgcaccacc acctcgaccc cggggtcggc caggacctcg
      661 gccagcggcc cggggtcgag ccgctcgtcc agcgggtcga ccagctcgac ccgcggcccg
      721 ccgtccccgt ccgggccgtc gaccaccacc ccgaccaggc agagcaaggc ccggtagcga
      781 ccctcggaca tgaactcggt gtcgatgccc aggcggccgg cggccctggc cgccctggcc
      841 agccccccca ggtccgcgcg cggggccagg ctcagccgag gcgccacgtg tccccgccgt
      901 ggagcagctt ggccaggtca ccccggccct gcctggtggt ggccgcctcc agctgctcgg
      961 acatgagctc gtcgtacacg ggccgctcga cgtcgcggaa caccccgatc ggggtcggcc
     1021 cgtcggcgcc gtgggacagc cggcagagca tgaaggccag gctcggctcg gcccggtgct
     1081 cgtcgtggac cagcagcgcg tcctcgccga cgtcggccac ctcgaccagc tcgatccggc
     1141 agtcgtcggt cagccggacc ccgcgctcgc cgtcggcgcc gaagcggatc ggcttgccgt
     1201 gctccagccg gatctggttg tcggcccggg tgtccggctc cttcagcagg tcgaaggcgc
     1261 cgtcgttgaa gatgttgcag ttctggtaga tctccacgaa cgcggtgccc tggtgggcgg
     1321 ccgccttgcc cagcacctcg gtgaggtggg cgcggtcgga gtcgatcgag cgggcgacga
     1381 aggtcgcctc cgcgcccagg gccacgctga ccgggttgaa cgggtggtcc agcgacccca
     1441 tcggcgtcga cttggtgatc ttgcccagct cgctggtggg ggagtactgg cccttggtca
     1501 gcccgtagat ctggttgttg aacagcagga tcttgaggtt gacgttgcgg cgcagggcgt
     1561 ggatgaggtg gttgccgccg atcgacaggg cgtccccgtc gccggtgatc acccacaccg
     1621 acaggtcagg gcgggtcacc gacagcccgg tggcgatcgc cggggcccgg ccgtggatcg
     1681 agtgcatccc gtaggtgttc atgtagtagg ggaagcgcga ggagcagccg atgcccgaga
     1741 tgaacacgat ccgctccctg gggatcccca gcgacggcat gaagccctgc acggccgaga
     1801 ggatcgcgta gtccccgcag ccggggcacc agcggacctc ctggtcgctc ttgaagtcct
     1861 tggcggtgag cttcacgggg gcgccgttgc cgtgcccgtt gcccgcagtc acttggtcgc
     1921 tccttcgtcg agcaggccga tgatggcgtc ggccagctcc tcggccttga acggcaaccc
     1981 ccggaccttg ttgtagccga cggcgtcgac caggaaccgg gagcggacca gcatgcgcag
     2041 ctggcccagg ttcatctccg ggatcaggac cttgtcgtag ccggccagca cctcgccgag
     2101 gttggccggg aacgggttga ggtggcgcag gtgggccacg gacacccggg cccccgaggc
     2161 ccggacccgc cgcaccgccg ccccgatcgg cccgtaggtc gagccccagc ccagcaccag
     2221 cagcttcgac ccgtcctggt ggtcgacctc gaggggggcg atgtcggcgg ccatgccggc
     2281 caccttggcc gcccggaggt tgaccatgcg gtcgtggttg tcggggtcgt agctgatgtt
     2341 gccggtgccg tcggccttct cgagcccgcc gatgcggtgc tcgaggccgg gcgtcccggg
     2401 gaccgcccag ggccgggcca gagtggcctc gtcgcgaagg tacggccagt agtcgccctc
     2461 gtggttgggc tcggtggcga actccacccc gatgtcgggc agcgagtcca cgtccggcag
     2521 cagccacggc tccgacccgt tggccaggta gccgtcggag agcaggaaga ccggcgtgcg
     2581 gtacttcagg gcgatccgcg cggcctcgat ggcggcgtgg aagcagtccg acggcgaacg
     2641 cggcgcgacg atcggcaccg gcgcctcgcc gttgcggccg aacatg
//
LOCUS       DASSWR010000302         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1340156, whole genome shotgun sequence.
ACCESSION   DASSWR010000302 DASSWR010000000
VERSION     DASSWR010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340156"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VFO47_05045"
     CDS             <1..970
                     /locus_tag="VFO47_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET8788094.1"
                     /translation="FTRAAGPGGAPTWELDFPRPAVDRMEYQLALRYPGAGPVLLPDP
                     ANPLRADGPFGEKSVIEFPGYRPPAWLAAAPRPAPSTTGLAVPSRVLGARIPVELWRS
                     PGGRRRRPGDPLPMLVVHDGPEFARYAALLHLLTVATGDGRLPPMRAALLAPVDRGEH
                     YSASPAWSRALVSEVLPALQALAPTPPGRRHLVGMGASLGALATLAAARTAPASFGGL
                     FLQSGSFFRPAADAHERDFPRFRRITRFVDDLTAGGGGSAEPASTTLTCGTVEENLHG
                     NRVVRDALAARGWQVALAEHRDAHNWVSWRDTFDPWLIGLLQELWV"
     gene            967..1707
                     /locus_tag="VFO47_05050"
     CDS             967..1707
                     /locus_tag="VFO47_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET8788095.1"
                     /translation="MRRDYVGLWSPAIGAEGGLLAYGHHGRPLLVFQSEQGRCSDYED
                     RGMIDVIAGLIEAGRVKVYSVDSYDAASWCDPNLPLEARATQHGRFEDWILNQVVPFI
                     YDDCGGPLDVMVTGASFGAFHAANFAFRRADLFPLAICQSGVYDAGQMGWGERGDAIY
                     FNNPMDYVANLGGDHLDWLRGRLSLLLICGQGQWEDTTGALESTKAFDRLLDEKGIRH
                     ETDLWGHDVPHDWPSWRAQLAHHLPRFV"
     gene            1724..>2560
                     /locus_tag="VFO47_05055"
     CDS             1724..>2560
                     /locus_tag="VFO47_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788096.1"
                     /translation="MTTTHLIGLLLGTEDDWPTAFEQYLGRLAPRITLDGETHEVATE
                     RVTIEPFDLRQPVRHALVIDRLAYWYDHPREWLKKIALMDRAYLLNNPFTFQAMEKHA
                     GYCAAIRLGFDVPATWLLPHKVPPENERFAPTASRYNRAFDLHAVGEQVGYPMYMKPF
                     HGGGWVNVYRIANPEELQAAYDASGRELMHVQAGVEGYDIFTRGLAIGPQTMVMHYDP
                     TRPLHLRYQVDHDFLTPEVGHEVVTFGLTVGSFFRWEFNSFEVIVRDGLCYPIDFANA
                     TPD"
BASE COUNT          339 a         1036 c          826 g          359 t
ORIGIN      
        1 cttcacccgg gccgccgggc ccgggggcgc gcccacctgg gagctggact tcccccggcc
       61 ggccgtcgac cggatggagt accagctcgc cctgcgctac ccgggcgccg gcccggtgct
      121 gctgcccgac ccggccaacc cgctccgggc cgacggcccc ttcggggaga agtcggtgat
      181 cgagttcccc ggctaccggc cgccggcctg gctggccgcc gccccccgcc ccgccccgtc
      241 caccaccggc ctggccgtgc ccagccgggt cctcggggcc cgtatcccgg tcgagctgtg
      301 gcgcagcccc ggcggccgcc gccgccggcc cggcgacccg ctgcccatgc tggtcgtcca
      361 cgacggcccc gagttcgccc gctacgccgc cctgctccac ctcctcaccg tcgccaccgg
      421 cgacggccgc ctcccgccga tgcgggccgc cctgctcgcc cccgtcgacc gcggcgagca
      481 ctactcggcc tccccagcct ggtcccgggc cctggtcagc gaggtcctgc ccgccctcca
      541 ggccctggcc ccgaccccgc ccggccgccg tcacctggtc ggcatggggg ccagcctggg
      601 cgccctggcc accctggccg ccgcccgcac cgccccggcc agcttcggcg gcctgttcct
      661 ccagtcgggc agcttcttcc ggcccgcggc cgacgcccac gagcgcgact tcccccggtt
      721 ccggcggatc acccgctttg tcgacgacct gaccgccggc ggcggcgggt cggccgagcc
      781 ggcctcgacc accctgacct gcgggacggt cgaggagaac ctccacggca accgggtcgt
      841 ccgcgacgcc ctggccgccc gcggctggca ggtcgccctg gccgagcacc gggacgcgca
      901 caactgggtg tcctggcgcg atacctttga tccctggctg atcgggctgc tccaggagct
      961 gtgggtgtga gaagggacta cgtcgggctg tggtcgcccg cgatcggggc cgagggcggg
     1021 ctgctcgcct acggccacca cgggcggccc ctgctggtct tccagtccga gcagggccgc
     1081 tgctccgact acgaggaccg cggcatgatc gacgtcatcg ccggcctgat cgaggccggc
     1141 cgggtcaagg tctactcggt cgacagctac gacgcggcca gctggtgcga ccccaacctg
     1201 cccctggagg cccgggcgac ccagcacggc aggttcgagg actggatcct caaccaggtc
     1261 gtcccgttca tctacgacga ctgcggcggc ccgctcgacg tcatggtcac cggggccagc
     1321 ttcggcgcct tccacgcggc caacttcgcc ttccgccggg ccgacctgtt ccctctggcc
     1381 atctgccaga gcggcgtcta cgacgccggc cagatgggct ggggggagcg cggcgacgcc
     1441 atctacttca acaaccccat ggactacgtg gccaacctcg gtggcgacca cctcgactgg
     1501 ctccggggcc ggctgtcgct gctgctcatc tgcggccagg gccagtggga ggacaccacc
     1561 ggggcgctcg agtcgaccaa ggccttcgac cgcctcctcg acgagaaggg catccgtcac
     1621 gagaccgacc tgtggggcca cgacgtgccc cacgactggc cgtcctggcg ggcccagctc
     1681 gcccaccacc tgccgcggtt cgtctagggg aagggcgcgc gccatgacca cgacccacct
     1741 gatcggcctg ctgctcggga ctgaggacga ctggccgacc gcgttcgagc agtacctggg
     1801 ccggctcgcc ccccgcatca ccctcgacgg cgagacccac gaggtggcca ccgagcgggt
     1861 caccatcgag cccttcgacc tgcgccagcc cgtccgccac gccctggtca tcgaccgcct
     1921 cgcctactgg tacgaccacc cgcgggagtg gctcaagaag atcgccctga tggaccgggc
     1981 ctacctgctc aacaacccct tcaccttcca ggcgatggag aagcacgccg gctactgcgc
     2041 cgccatccgg ctcgggttcg acgtgcccgc gacctggctg ctgccccaca aggtcccgcc
     2101 cgagaacgag cgcttcgccc cgaccgcctc gcgctacaac cgggccttcg acctgcacgc
     2161 ggtgggggag caggtcggct acccgatgta catgaagccg ttccacggcg gcggctgggt
     2221 caacgtctac cgcatcgcca acccagaaga gctgcaggcc gcctacgacg cctccgggcg
     2281 ggagctgatg cacgtccagg ccggggtcga gggctacgac atcttcaccc gtgggctggc
     2341 catcggcccc cagacgatgg tcatgcacta cgacccgacc aggccgctgc atctgcgcta
     2401 ccaggtggac cacgacttcc tcactcccga ggtgggccac gaggtcgtca ccttcggcct
     2461 gaccgtcggg tcgttcttcc gctgggagtt caacagcttc gaggtgatcg tccgcgacgg
     2521 cctctgttac ccgatcgact tcgccaacgc cacccccgac
//
LOCUS       DASSWR010000303         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_265852,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000303 DASSWR010000000
VERSION     DASSWR010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265852"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..>2038)
                     /locus_tag="VFO47_05060"
     CDS             complement(143..>2038)
                     /locus_tag="VFO47_05060"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET8788097.1"
                     /translation="PDGSGWSPRFMVRTVEVTGDGSCAAVAAEDPEAGLALVTRLRLH
                     PSGVLEAGHTLRNLGDGPYLVTQLGVSLPLPARAVELLDLTGRWCRERIPQRHPLPHG
                     AWVREGRHGRTGHDATLVLAAGSPGFGFRSGEVWGIHLGWSGDAVHWAERNADGHAGI
                     GAAELLGPSEVALGPGESYATPPVYAGYSADGLDGLSAAYHRFLRDRPSHPRTPRPVV
                     LNTWEAVYFDHRLERLTDLAEVAARLGVERFVLDDGWFGNRRNDTAGLGDWYVAADVW
                     PDGLNPLIGHVRKLGMEFGLWVEPEMVNPDSDLFRAHPDWVLSGSGQPGRLPQPWRHQ
                     QVLDLANPDCYAFIRDRLDALLGEHDIAFLKWDHNRDLIEAGHAGRPGVHAQTLAVYR
                     LLDELRERHPGVEIESCASGGGRVDLGILARTDRVWASDTNDALERQHIQRWTQLLVP
                     PELVGSHVGPPRAHTTGRTHDLAFRVATALFGHFGIEWDIASASEEEQAALAEAIACY
                     KRLRPLLHSGQVVRADHPNPAAYLHGVVAPDRAEALFAYVQLTASAFETPGLARLCGL
                     DPGRAYRVTPLTVAGAPQTKQAVGPRWTETGGVTLNGRVLEEVGLPLPVLMPEQALLL
                     QLTQEAS"
BASE COUNT          260 a          724 c          805 g          249 t
ORIGIN      
        1 acgaagccgg tatcggcatc ggccctccca gacagccgaa accgccagtc cccagagagg
       61 tcaatggccg gcgcgtcgga ctggaaccac gcccgtggcg ggtgcgcacc gaccgtgggt
      121 tggacatcct ccaggccaga agtcatgacg cctcctgggt cagctggagc agcagggcct
      181 gctcgggcat caggacgggc aggggcaggc cgacctcctc gaggacccgg ccgttcaggg
      241 tgaccccgcc ggtctcggtc cagcgggggc cgaccgcctg cttggtctgg ggggcgccgg
      301 ccacggtcag cggcgtgacc cggtaggcgc ggccggggtc caggccgcac agccgggcca
      361 gccctggggt ctcgaacgcc gaggcggtca gctgcacgta ggcgaacagc gcctcggccc
      421 ggtcgggagc gaccaccccg tgcaggtagg cggccgggtt ggggtggtcg gccctgacga
      481 cctgcccgct gtggagcagc ggccgcagcc gcttgtagca ggcgatcgcc tcggccagcg
      541 ccgcctgctc ctcctcggag gccgaggcga tgtcccactc gatgccgaag tggccgaaca
      601 gcgccgtcgc gacccggaag gcgaggtcgt gggtgcggcc ggtggtgtgg gcccgcggcg
      661 ggccgacgtg gctgccgacc agctcgggcg ggaccaggag ctgggtccag cgctggatgt
      721 gctggcgctc cagggcgtcg ttggtgtcgc tggcccagac ccggtcggtg cgggcgagga
      781 tgcccaggtc gacccggccg cccccggagg cgcagctctc gatctcgacc ccggggtggc
      841 gctcgcgcag ctcgtccagc agccggtaga cggccagggt ctgggcgtgg acccccggcc
      901 ggccggcgtg gccggcctcg atcaggtcgc ggttgtggtc ccacttgagg aaggcgatgt
      961 cgtgctcgcc cagcagcgcg tccagccggt cgcggatgaa ggcgtagcag tcggggttgg
     1021 ccaggtcgag cacctgctgg tggcgccacg gctggggcag ccgccctggc tggcccgacc
     1081 cggacaggac ccagtcgggg tgggcccgga acaggtcgga gtcggggttg accatctccg
     1141 gctcgaccca cagcccgaac tccatgccca gcttccggac gtggccgatc agcgggttga
     1201 ggccgtccgg ccagacgtcg gcggccacgt accagtcgcc gagcccggcg gtgtcgttgc
     1261 ggcggttgcc gaaccagccg tcgtcgagca cgaaccgctc gaccccgagc cgggcggcca
     1321 cctcggccag gtcggtgagc cgctccaggc ggtggtcgaa gtagacggcc tcccaggtgt
     1381 tgagcacgac cggccggggc gtgcgcgggt gggacggccg gtcgcgcagg aagcggtggt
     1441 aggcggcgct gaggccgtcc aggccgtcgg cgctgtagcc ggcgtacacc ggcggggtgg
     1501 cgtacgactc gccggggccg agggccacct cggaggggcc gagcagctcg gccgcgccga
     1561 tcccggcgtg gccgtcggcg ttgcgctccg cccagtggac cgcgtccccg ctccagccca
     1621 ggtggatccc ccagacctcg ccggagcgga acccgaaccc ggggctgccg gcggccagga
     1681 ccagggtggc gtcgtggccg gtgcgcccgt ggcggccctc gcgcacccag gcgccgtggg
     1741 gcagcgggtg ccgctggggg atgcgctcgc ggcaccaccg gccggtcagg tcgagcagct
     1801 ccaccgcccg ggccggcagg ggcaggctga ccccgagctg tgtgaccaga tagggcccgt
     1861 cgcccaggtt gcggagggtg tggccggcct caaggacccc cgagggatgc agccgcagcc
     1921 gcgtgaccag ggccagcccg gcctcggggt cctcggccgc gacggcggcg cagctgccgt
     1981 cgccggtgac ctcgaccgtc cggaccatga accgcggcga ccagccgctc ccgtccgg
//
LOCUS       DASSWR010000304         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_633638,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000304 DASSWR010000000
VERSION     DASSWR010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633638"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..275)
                     /locus_tag="VFO47_05065"
     tRNA            complement(194..275)
                     /locus_tag="VFO47_05065"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Tyr,seq:gta)
     gene            complement(330..713)
                     /locus_tag="VFO47_05070"
     CDS             complement(330..713)
                     /locus_tag="VFO47_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788098.1"
                     /translation="MGFSRQFTGGFEDVADARRFVAATLLRDTELYEAARLLVDEAIT
                     NPLLHSEACETVRAFQVTIAFRPHVLHVEVHDGGAPLPLDRDGARPVSVAGRSLELFD
                     TLALVWDSSPEPDRPGHVVWFELEA"
     gene            832..1320
                     /locus_tag="VFO47_05075"
     CDS             832..1320
                     /locus_tag="VFO47_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET8788099.1"
                     /translation="MADSSFDVVAKVDRQEVDNAVNQARKELSQRFDFKNTGTSLAWA
                     GEGVVIVSKTDQRALAALDVFKEKLVKRSVSLKALTSSEPRPAGGGTYRVECSFLQGI
                     PEDKAKALAKTIRQSGLKVQAQVQGDQLRVTGKSKNDLQQAIALLKERDEGLPLRFTN
                     YR"
     gene            1414..1773
                     /locus_tag="VFO47_05080"
     CDS             1414..1773
                     /locus_tag="VFO47_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8788100.1"
                     /translation="MQYALLIYASEQEWASQTEEESQAQFQEYMAFTKDIVDRGIYKG
                     GEALQPTATATTVQVRDGERLTTDGPFAETKEQFGGFYVVEAKDLDEAIEIAARIPDV
                     RRGSIEVRPVMETGMPS"
     gene            1797..>2479
                     /locus_tag="VFO47_05085"
     CDS             1797..>2479
                     /locus_tag="VFO47_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8788101.1"
                     /translation="MAEETRALVERVFREESGQAVATLIRLLGDFDLAEEAVQEAFLV
                     AVERWPGGGVPDRPGAWIITTARNKAIDRLRRERGLAERRATLALLEADRGQDSEMHT
                     IADDRLRLIFTCCHPALSPEARVALTLRTLGGLSTPEIARAFMVPEATMAQRLVRAKR
                     KIRDAAIPYRVPPDHLLPERLGSVLAVLYLIFNEGYAASAGDALVRRELCAEAVRLGR
                     VLAELMPDEG"
BASE COUNT          404 a          841 c          905 g          329 t
ORIGIN      
        1 ctacatccac caactcccgc agactgcgcc ccggctggct aggctgatcg agggtgtgtt
       61 cggcccagcg gcgcgggcca cgcggtcggt gtcggcaccg cgtcgggcgg agggaaaccg
      121 gagggaaact cgaccctcga acccggcttg attggacggc ccgagaccgc atagttgctg
      181 gtcaggggca gtgtggcagg gacaggattc gaacctgtgt aggcaatgcc ggcgatttta
      241 cagaccgctc cctttggccg ctcgggcacc ctgccggacc gagggcggat gataccggcc
      301 cccggagcgt cccaacaacc gggtgggagt caggcttcca gctcgaacca gaccacatgg
      361 ccggggcggt ccggctcggg ggaggagtcc cagacgaggg ccagggtgtc gaacagctcg
      421 aggctgcggc cggcaaccga gaccgggcgg gcgccgtcgc ggtcgagcgg cagcggggcg
      481 ccgccgtcat ggacctcgac gtggagcacg tggggacgga aggcgatggt cacctggaag
      541 gcgcggacgg tctcgcaggc ctcgctgtgc agcagcgggt tggtgatggc ctcgtcgacc
      601 aggagccggg ccgcctcgta gagctcggtg tcgcgcagca gggtggcggc cacgaaccgc
      661 cgggcgtcgg ccacgtcctc gaagccgccg gtgaactgcc gcgagaaccc cacacccacc
      721 tcccctgagc gaacggagtg gaacgactac ccgggtttgt cccccatcaa ccgccctgcg
      781 cgacccggct gtctgctagc gtccccgagg atctcgagga aggaggatgc cgtggccgac
      841 tcctcgttcg acgtcgtcgc caaggtcgac cgtcaggagg tcgacaacgc cgtcaaccag
      901 gcaaggaagg agctgtcgca gcgcttcgac ttcaagaaca ccggcacctc gctggcctgg
      961 gcgggtgagg gggtggtgat cgtctccaag accgaccagc gggccctggc cgccctcgac
     1021 gtgttcaagg agaagctggt gaagcggtcg gtgtcgctca aggcgctgac ctcctcggag
     1081 ccgcggcctg cgggcggcgg gacctaccgg gtggagtgct cgttcctcca gggcatcccc
     1141 gaggacaagg ccaaggcact ggccaagacc atccgccagt ccgggctcaa ggtgcaggcc
     1201 caggtccagg gcgaccagct gcgggtcacg ggcaagagca agaacgacct ccagcaggcg
     1261 atcgccctgc tcaaggagcg cgacgagggc ctgccgctgc gcttcaccaa ctatcgttga
     1321 ggaatctggc ggggggtgtc gattccggcg cgggccgctc gtcggttgca tagagggcgg
     1381 cgaaccgcgc cgccccggtc ccggggaggg agcatgcagt acgcactgct gatctacgcg
     1441 agcgagcagg aatgggccag ccagaccgag gaggagtccc aggcccagtt ccaggagtac
     1501 atggccttca ccaaggacat cgtagaccgc ggcatctaca agggcggcga ggccctgcag
     1561 ccgaccgcca ccgcgactac cgtgcaggtg cgcgacggcg agcggctaac caccgacggg
     1621 ccgttcgccg agaccaagga gcagttcggc gggttctacg tggtcgaggc aaaggacctg
     1681 gacgaggcga tcgagatcgc ggccaggatc cccgacgtcc ggcgcgggtc gatcgaggtc
     1741 cggccggtca tggagacggg catgccgtcc tgaggacgag aatccggggg gtgagggtgg
     1801 ccgaggagac ccgtgcgctg gtcgagcggg tgttccggga ggagtcaggg caggccgtgg
     1861 ccaccctcat ccgcctcctc ggcgacttcg acctggccga ggaggccgtc caagaggcct
     1921 tcctggtcgc cgtcgagcgc tggccgggcg gcggcgtgcc cgaccggccc ggggcctgga
     1981 tcatcaccac cgcccgcaac aaggccatcg accggctccg ccgcgagcgc gggctggccg
     2041 agcgccgggc caccctggcc ctgctcgagg ccgaccgcgg ccaggacagc gagatgcaca
     2101 ccattgccga cgaccggctg cggctgatct tcacctgctg ccacccggcc ctgtcgcccg
     2161 aggccagggt cgccctcacc ctccgcaccc ttggggggct gtcgacgccc gagatcgccc
     2221 gcgccttcat ggtcccggag gcgaccatgg cccagcggct ggtccgggcc aagcgcaaga
     2281 tccgcgacgc cgccattccc tatcgggtgc cgcccgacca cctgctgccg gagcggctcg
     2341 ggtcggtgct ggccgtgctc tacctgatct tcaacgaggg ctacgccgcc agcgccggcg
     2401 acgccctggt gcgccgggag ctgtgcgccg aggcggtccg cctcgggcgg gtcctggccg
     2461 agctgatgcc agacgaggg
//
LOCUS       DASSWR010000305         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_68650,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000305 DASSWR010000000
VERSION     DASSWR010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68650"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VFO47_05090"
     CDS             complement(<1..898)
                     /locus_tag="VFO47_05090"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET8788102.1"
                     /translation="MAQITMAKALNTALRDAMEADDRVVLLGEDVGTLGGVFRITDGL
                     LKDFGEQRVMDTPLAESAIMGVSIGLALRGYRPVPEMQFDGFSYPALDQIISHLAKYR
                     NRSAGTQAMPVVCRIPYGGGIGAVEHHSESPETIYCHTAGLKVVTPSSAADAYSLLRQ
                     SIEADDPVIFLEPKRRYWAKQEVDLPVATEPIGKARVLRQGSDLTLVAYGPMVKVAMD
                     AAEAAEEEGVGSLEVIDLRSLVPLDEETLVASAARTGRMVVVHEAPQFLGMGAEVAAR
                     VSEAAFFHLEAPVLRCTGLDIPY"
     gene            complement(910..1986)
                     /gene="pdhA"
                     /locus_tag="VFO47_05095"
     CDS             complement(910..1986)
                     /gene="pdhA"
                     /locus_tag="VFO47_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET8788103.1"
                     /translation="MDQTDEQVRLLGEDGTLLQHDTYKVELSDEDLCDLYRQLVVVRR
                     IDVEGNNLQRQGQLGIWAPCLGQEAAQVGSARALGPDDFVFPSYREHGVVYVRAGMEV
                     LSVLGLYRGANLSGWDPQRHKISQYSIPIGTQALHAVGYAVGAKWDGAEVCAVAYFGD
                     GATSEGDVSEAFNFAAVHSAPVVFFCQNNQWAISVPLAKQMAGPVYKRALGFGFPGVQ
                     VDGNDVLAVYAVTRAAAERARDGGGPTLIEAITYRLGAHTTTDDPTRYRTSDELDVWT
                     AREPIGRYRAFLERAGLWSETLEGDVQAEADQVAARVRSTVEDMPDPPLSHLVDHVYA
                     DPPATLVAQWRQLEEFEAQFQAEA"
BASE COUNT          293 a          719 c          777 g          311 t
ORIGIN      
        1 ggtacgggat gtccaggccg gtgcagcgca gcacgggggc ctccaggtgg aagaaggcgg
       61 cctcgctgac ccgggccgcg acctcggccc ccatgccgag gaactgcggg gcctcgtgca
      121 cgaccaccat ccggccggtc cgggcggcgg aggcgaccag ggtctcctcg tccagaggga
      181 cgaggctgcg caggtcgatg acctccagcg acccgacgcc ctcctcctcg gcggcctcgg
      241 ccgcgtccat ggccactttc accatcgggc cgtaggcgac cagggtcagg tcgctgccct
      301 ggcgcagcac gcgggccttg ccgatcggct cggtggcgac cggcagatcg acctcctgct
      361 tggcccagta gcggcgcttg ggctcaagga agatcaccgg gtcgtcggcc tcgatggact
      421 ggcgcagcag cgagtaggcg tcggcggccg aggagggcgt gaccaccttg aggccggcgg
      481 tgtggcagta gatggtctcg gggctctcgg agtggtgctc gacggcgccg atgcccccgc
      541 cgtaggggat gcggcagacc accggcatgg cctgggtgcc ggccgagcgg ttccggtact
      601 tggccaggtg gctgatgatc tggtcgaggg ccgggtagct gaagccgtcg aactgcatct
      661 cgggcacggg ccggtagccg cgcagggcca gcccgatgct gacccccatg atggccgact
      721 cggccagcgg ggtgtccatc acccgctgct cgccgaagtc cttgagcagg ccgtcggtga
      781 tgcggaagac cccgccgagg gtgccgacgt cctcgcccag cagcaccacc cggtcgtcgg
      841 cctccatggc gtcgcgcagg gcggtgttga gcgccttggc catcgtgatc tgcgccatcg
      901 gtgcctccct cacgcctcgg cctggaactg ggcctcgaac tcctcgagct ggcgccactg
      961 ggcgaccagg gtggccggcg ggtcggcgta gacgtggtcg accaggtggg acaggggcgg
     1021 gtcgggcatg tcctcgacgg tcgaccggac ccgagccgcg acctggtcgg cctcggcctg
     1081 gacgtcgccc tccagcgtct ccgaccacag cccggcccgt tccaggaacg cccggtagcg
     1141 gccgatcggc tcgcgggccg tccagacgtc gagctcgtcg gaggtccggt agcgggtcgg
     1201 gtcgtcggtg gtggtatggg ccccgagccg gtaggtgatg gcctcgatca gggtcggtcc
     1261 gccgccgtcg cgcgcccgct cggccgccgc cctggtcacg gcgtagacgg ccagcacgtc
     1321 gttgccgtcg acctggaccc cggggaagcc gaagccgagg gcccgcttgt agacggggcc
     1381 ggccatctgc ttggccagcg gcaccgagat ggcccactgg ttgttctggc agaagaacac
     1441 caccggggcg ctgtgcacgg cggcgaagtt gaaggcctcg ctgacgtcgc cctcgctggt
     1501 ggcgccgtcg ccgaagtagg cgacggcgca gacctcggcc ccgtcccact tggccccgac
     1561 ggcgtagccg acggcgtgca gggcctgggt gccgatgggg atggagtact gggagatctt
     1621 gtggcgctgg gggtcccagc cggacaggtt ggccccgcgg tacaggccga ggaccgacag
     1681 cacctccatg ccggcgcgga cgtagacgac gccgtgctcg cggtaggagg ggaacacgaa
     1741 gtcgtccggc ccgagggctc tggcgctgcc gacctgggcc gcctcctggc cgaggcaggg
     1801 cgcccagatg ccgagctgcc cctggcgctg gaggttgttg ccctcgacgt cgatgcgccg
     1861 caccaccacg agctggcggt acaggtcgca gaggtcctcg tcgctcagct cgaccttgta
     1921 agtgtcgtgc tggaggagcg tcccgtcctc gccgagcagc cgaacctgct cgtcggtctg
     1981 gtccatgcca gtgtctcccg cgtcgttgcg ccgactctcc tgatcctact cgtaggaatg
     2041 ggcgcaagac acaagacgcc ccggaaccga agagcgcagg tccggcggac tcaatacaca
//
LOCUS       DASSWR010000306         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_802970,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000306 DASSWR010000000
VERSION     DASSWR010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802970"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFO47_05100"
     CDS             complement(<1..192)
                     /locus_tag="VFO47_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8788104.1"
                     /translation="MADYDAIVCGGGSNGLAAAIRLAEAGWSVCLLEANDDLGGAART
                     LESTLPGFKHDFGAAFFPLV"
     gene            319..1410
                     /locus_tag="VFO47_05105"
     CDS             319..1410
                     /locus_tag="VFO47_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET8788105.1"
                     /translation="MTALGQVMDPEIHRPITDLDMVKDVSIDPGGLVTVEVLLTVAGC
                     PLKDRITRDVTAAVSPLPGVTGVKVDLGVMTEDQRQAMVTRLRGPGADQQQKKITFWG
                     DESTTRVLAVASGKGGVGKSSVTVNLAAGLAAQGHRVAVIDCDIYGFSVPRMLGATGQ
                     PVGFNGMIMPLEAHGVRVISIGFFLPPGKPVAWRGPMLHRAIQQFLTDVYWGDLDYVL
                     ADLPPGTGDVSISLAQMLPGAEMLLVTTPQEAAERVAVLAGNFAQQNGMKVTGVIENM
                     SYFVCPSCDERHQIFGSGGGETLAGELGTTLLGQIPIDVRLREGADTGKPLVISDPDA
                     PASQALLEVAAALPPLPRSLVGKRLTLLT"
     gene            complement(1472..1909)
                     /locus_tag="VFO47_05110"
     CDS             complement(1472..1909)
                     /locus_tag="VFO47_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET8788106.1"
                     /translation="MFNVGLGEIVAIVLVALLLFGPERLPEMARQAGRWLGRLRLTTQ
                     DALDQLKDETDLKDLNLPDLRIGSLRSQARDYVRELMDVEGQMAELEREREQLKAALD
                     AEQNGGPKGTGDGAAEDARPAAEGDSTSSTADLGPPPVDPEAT"
     gene            complement(1922..>2279)
                     /locus_tag="VFO47_05115"
     CDS             complement(1922..>2279)
                     /locus_tag="VFO47_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET8788107.1"
                     /translation="IDEALEVATALVDRKPIRVPFLGVEAGAELTPELVAHYRLGNRA
                     GALVGRVAPRSPAAEAGLRRGDLIIRLGNQPIPARDQLTVAERRAEIGVPVEVTVVRR
                     GQQLVLPVTPTDRPER"
BASE COUNT          305 a          856 c          795 g          323 t
ORIGIN      
        1 caccagcggg aagaaggcgg caccgaagtc gtgcttgaac ccgggcaggg tgctctccag
       61 ggtcctggcc gccccgccga ggtcgtcgtt ggcctccagc aggcacaccg accagccggc
      121 ctcggccagg cggatggcgg cggcgagccc gttcgagccc ccgccgcaga cgatcgcgtc
      181 gtagtcggcc atcaccccga gcctactctg ctcgcgacgg cgaggcgtcc cggtggtcgc
      241 tacactccag gaagaagggc ggtttcgccc ggcacggctc agaagggacc ggttctgatg
      301 gtcacgcatg atgaggtcat gacagcgctc gggcaggtga tggacccgga gatccaccgg
      361 cccatcaccg acctggacat ggtcaaggac gtctccatcg acccgggcgg cctggtcacc
      421 gtcgaggtgc tgctcaccgt cgccggctgc ccgctgaagg accggatcac cagggacgtc
      481 accgcggccg tgtccccgct gcccggggtc acgggggtca aggtcgacct cggggtgatg
      541 accgaggacc agcggcaggc catggtcacc cgcctgcgcg gaccgggcgc ggaccagcag
      601 cagaagaaga tcaccttctg gggcgacgag tcgaccacca gggtgctagc cgtcgcctcg
      661 ggcaagggcg gggtcggcaa gtcctcggtg accgtcaacc tggccgccgg cctggccgcc
      721 caggggcacc gggtggcggt gatcgactgc gacatctacg gcttctcggt tccccgcatg
      781 ctcggcgcca ccggccagcc ggtcggcttc aacggcatga tcatgccgct ggaggcgcac
      841 ggggtgcggg tcatctcgat cggcttcttc ctgcccccgg gcaagccggt ggcctggcgg
      901 ggcccgatgc tgcaccgggc catccagcag ttcctcaccg acgtctactg gggcgacctt
      961 gactacgtgc tcgccgacct gccgcccggc accggcgacg tctccatctc gctggcccag
     1021 atgctccccg gggccgagat gctgctggtc accacccccc aggaggcggc cgagcgggtg
     1081 gccgtgctgg ccggcaactt cgcccagcag aacgggatga aggtcaccgg cgtgatcgag
     1141 aacatgagct acttcgtctg cccgtcctgc gacgagcgcc accagatctt cggcagcggc
     1201 ggcggcgaga ccctggccgg cgagctcggc accaccctgc tcggccagat cccgatcgac
     1261 gtgcgcctgc gcgagggcgc cgacacgggc aagccgctgg tcatctccga ccccgacgcc
     1321 ccggccagcc aggccctgct cgaggtcgcg gccgccctgc cgcccctgcc ccgttcgctg
     1381 gtcggcaagc ggctgacgct gctgacatag gctcggagtc ggccttcggc cgacgatcca
     1441 cccgttccga ccggcaggtt cgccggtcgg gtcaggtcgc ctcggggtcg accgggggcg
     1501 gtccgaggtc ggcggtgctg gacgtggagt cgccctcggc ggcgggcctg gcgtcctccg
     1561 cggccccgtc cccggtgccc ttggggccgc cgttctgctc ggcgtcgaga gcggccttga
     1621 gctgctcccg ctcccgttcc agctcggcca tctggccctc gacgtccatc agctcgcgca
     1681 cgtagtcgcg ggcctgggag cgcagcgacc cgatccggag gtcgggcagg ttcagatcct
     1741 tgaggtcggt ctcgtccttg agctggtcga gcgcgtcctg ggtggtcagc cgcagccggc
     1801 ccagccagcg cccggcctgc ctggccatct cgggcagccg ctcgggcccg aacagcagca
     1861 gcgcgaccag cacgatggcc acgatctcgc ccagcccgac gttgaacacg gcccgctccc
     1921 cctaccgctc cggccggtcg gtcggggtga ccggcaggac gagctgttgc ccgcggcgga
     1981 ccaccgtgac ctcgaccggc acgccgatct cggcccgtcg ctcggccacg gtcagctggt
     2041 ccctggccgg gatcggctgg ttgcccaagc ggatgatcag gtcgccccgg cgcaggcccg
     2101 cctcggcggc cgggctgcgc ggcgccaccc ggccgaccag cgccccggcc cggttgccga
     2161 gccggtagtg ggcgaccagc tccggggtca actccgcgcc cgcctccacg cccaggaacg
     2221 gcaccctgat cggcttgcgg tcgaccagcg ccgtcgccac ctccagggcc tcgtcgatc
//
LOCUS       DASSWR010000307         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_549077,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000307 DASSWR010000000
VERSION     DASSWR010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549077"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VFO47_05120"
     CDS             <1..1271
                     /locus_tag="VFO47_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788108.1"
                     /translation="AGALAYAFTPHRMFQLTSHFNAVMWWAFPAALLAFEVMMERRRA
                     GRPWGWPAAGLAAVTLTVATSGEFHLILYLTGLLGFLVVWVLVAGLAGRRGVPWGPLA
                     VVVATLAVACAYVLLVFQAVFKGSVAGENGSWQQVVLYSPSSVWAMVHKTFGTQGEGL
                     IYVGWPLLVLAGIGLVAVVARRRAALAYAVLAVPLVLLTYGARANVAGVQVYRFLFDH
                     LPFLSLQRVPERLMVVTVLILVLLAVTALDVAAGLLAGRRRALVAAAVVLSLVTVGLL
                     ADYRVSRNRLEPDRADNRVVAALRAAGDGAGPVLGVPILGQAVTWNSVSTYLGAQSRR
                     RTLNAYNQTPAPWLTDRVARLEPLNRGLADPAALDVLRQTGTRQVVVVDEPRVFAPGE
                     WQVTVDRLVASGQFRLVARDGPLALLERTG"
     gene            complement(1517..2407)
                     /locus_tag="VFO47_05125"
     CDS             complement(1517..2407)
                     /locus_tag="VFO47_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8788109.1"
                     /translation="MSVLVVARWTVLEARRRRLLAAGVVLSVAFVALFALGYGLLYHD
                     QQRAVLEAETLGAGVLSAREELLAVSTLMLVLGLYGVQFLGALLGLFLGVASVSPEID
                     SGALHAVLARPLSRLQYLLGRFLALAGLLAAYVLVMSGALLLTARVVAGFTPGDARRV
                     VGLMLLEVLILLAVSLLGSTVLPTLANGVVMLALFGLAWLGGILGFVATIPPGNELLA
                     NLGTAAGLLLPADAVWRGASFHVLPPGFLAATSLAGGEVDGPPFVSTAPMAPAMLAWA
                     LAYPAACLALAAAAFRRRDL"
     gene            complement(2454..>2863)
                     /locus_tag="VFO47_05130"
     CDS             complement(2454..>2863)
                     /locus_tag="VFO47_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788110.1"
                     /translation="REVRELVRELAAGGVTVFLNSHLLTEVEAVCDRVAIVSQGRVLA
                     SGPLDDLAGAATQLRLLLDRADRDVLDLLGRRGRVVAVEGTTVTLAVETLDVAPDLAR
                     LLVGAGYQLYGMVPVQRSLEDVFVDLVGEVREQ"
BASE COUNT          375 a         1058 c         1089 g          341 t
ORIGIN      
        1 cggccggcgc cctggcctac gccttcaccc cccaccggat gttccagctc accagccact
       61 tcaatgccgt catgtggtgg gccttcccgg cggcgctgct ggccttcgag gtcatgatgg
      121 aacggcggcg agcggggcgg ccatggggct ggccggcggc cgggctggcc gcggtcaccc
      181 tgaccgtggc cacgagcggc gagttccacc tcatcctgta cctgaccggg ctgctcgggt
      241 tcctggtcgt gtgggtgctg gtggccggcc tggccggccg gcgcggcgtg ccctgggggc
      301 cgctggccgt ggtcgtggcc accctcgcgg tggcctgcgc ctacgtcctg ctcgtcttcc
      361 aggccgtgtt caaggggtcg gtggccggcg agaacggcag ctggcagcag gtcgtcctct
      421 actcccccag ctcggtgtgg gcgatggtcc acaagacctt cggcacccag ggcgaggggc
      481 tgatctacgt cggctggccg ctgctcgtgc tggccgggat cggcctggtc gcggtcgtgg
      541 ccagacggcg ggccgcgctc gcctatgccg tcctggccgt gcccctggtg ctgctcacct
      601 acggggcgcg ggcgaacgtc gccggggtcc aggtgtaccg gttcctcttc gaccacctgc
      661 cgttcctctc cctgcagcgg gtcccggagc ggctgatggt ggtgacggtc ctgatcctgg
      721 tcctgctggc cgtgaccgcc ctggacgtgg ccgccggcct gctcgccggg cggcgccgcg
      781 ccctggtcgc cgcggcggtc gtgctgtcgc tggtcacggt cggcctgctg gccgactacc
      841 gggtctccag gaaccgcctg gagccggaca gggcggacaa ccgggtggtg gcggcgctgc
      901 gggcggccgg ggacggcgcc gggccggtgc tgggggtgcc gatcctgggc caggcggtca
      961 cctggaactc ggtcagtacc tatctgggag cgcagagccg gcgccggacg ctgaacgcct
     1021 acaaccagac cccggccccc tggctgaccg accgggtggc ccgcctggag ccgctcaacc
     1081 gcggcctggc cgacccggcc gccctggacg tgctccgcca gaccggcacc cgccaggtcg
     1141 tggtggtcga cgagccgcgc gtgttcgccc ccggcgagtg gcaggtcacc gtcgaccgcc
     1201 tggtcgccag cggccagttc cgcctggtgg ctcgcgatgg tcccctggcc ctgctcgagc
     1261 ggaccggctg acccaacgtg gccacgcggg gctcagcctg gccacccggg ccagcctgac
     1321 tacccggggg gcggcgggct accccgggcc cgggcggctc ccccgggccc cgggccgggg
     1381 catggcggaa ccacaaacct ccggtgcggg cccccactcg ggagggtagc cgacccggga
     1441 ggagggggtg cgcggttgtc cacacccccc gtcgcaacga cgccggcgcg gccatccaga
     1501 cgcccggtgc gaactctcag aggtcgcggc ggcggaacgc ggcggcggcc agggcgaggc
     1561 aggccgccgg gtaggcgagc gcccaggcga gcatggccgg ggccatcggg gcggtggaga
     1621 cgaacggcgg gccgtccacc tcgcccccgg ccagggaggt ggcggccagg aagccgggcg
     1681 ggaggacgtg gaacgacgct ccgcgccaga cggcgtcggc cgggagcagg aggccggcgg
     1741 ccgtgcccag gttggccaac agctcgttgc cgggcgggat cgtggccacg aacccgagga
     1801 tgccgccgag ccaggccagg ccgaacaggg caagcatgac caccccgttg gccagcgtcg
     1861 gcagcaccgt gctgccgagc aggctcaccg cgagcaggat caggacctcc agcagcatga
     1921 gcccgaccac ccggcgggcg tcgccggggg tgaagccggc gacgacccgg gcggtgagca
     1981 ggagggcgcc gctcatcacg agcacgtagg cggcgagcag cccggccagg gcgaggaagc
     2041 ggccgaggag gtactggagg cggctgaggg gacgggcgag cacggcgtgg agggcgcccg
     2101 agtcgatctc cggggagacc gaggcgaccc ccaggaacag ccccagcagc gcccccagga
     2161 actggacccc gtagagcccg agcaccagca tcagggtcga gacggccagg agctcctcgc
     2221 gggccgacag gacgccggcg ccgagcgtct cggcctccag cacggcccgc tgctggtcgt
     2281 ggtagaggag gccgtacccg agcgcgaaca gggcgacgaa ggcgacgctg agcaccaccc
     2341 ctgccgccag gagccggcgc cggcgggcct ccagcaccgt ccagcgggcc accaccagga
     2401 cgctcaccgg cgcgccctcc gatccaccag ggtctcgtat gcgcccgccc tgctcattgc
     2461 tcgcgcacct ccccgaccag gtcgacgaag acgtcctcca gcgaccgctg caccggcacc
     2521 atcccgtaca gctggtagcc ggccccgacc agcagccggg ccaggtcggg ggcgacgtcc
     2581 agggtctcga cggccagggt gacggtggtg ccctcgaccg cgacgacccg gccgcgccgt
     2641 cccagcagat cgagcacgtc gcggtcggcg cggtccagca gcagccgcag ctgggtcgcg
     2701 gccccggcca ggtcgtccag gggcccgctg gccaggaccc ggccctggct gacgatcgcc
     2761 acccggtcgc agaccgcctc gacctcggtg agcaggtgcg agttgaggaa cacggtgacc
     2821 ccgccggcgg ccagctcccg gaccagctcg cggacctcgc ggc
//
LOCUS       DASSWR010000308         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1001125, whole genome shotgun sequence.
ACCESSION   DASSWR010000308 DASSWR010000000
VERSION     DASSWR010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001125"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFO47_05135"
     CDS             complement(<1..250)
                     /locus_tag="VFO47_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET8788111.1"
                     /translation="MNGNLDRRRFVQLAALAGAAALAGCGTSSPLGNRQPIRLGYISP
                     QSGPLFAFGEADSFVVENAKGAFKDGLEIGGRAHPVEIL"
     gene            complement(252..1307)
                     /locus_tag="VFO47_05140"
     CDS             complement(252..1307)
                     /locus_tag="VFO47_05140"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HET8788112.1"
                     /translation="MPPFTYDALPGRVVFGVGAADRLGEEVDRLGARRVLAIAGKRAV
                     DGLVERLGGRWAASFTDVQQHVPVEAAARAVAAAAEAGADCLVAMGGGSATGMAKAVA
                     LEHEAPIVAVPTTYAGSEVTPIYGLTGPEGKRTGRDPRVLPRTVLYDPALTTGLPPEV
                     TGPSGMNALAHCVEALYAPGANPVTALLATEGARVLASGLPVAVTDPADLAARSDTLL
                     GAWLAGAALAAAGVGIHHQLCHLLGGAYRLPHAELHAVMLPHAVRFVAPAARPQLARL
                     AGGLGVDDVAGGIWDLGRRLGTPASLAELGLAEAELDRAAELAVAKVVQTPRRAGISE
                     LRDLLEDAWQGRRPPPT"
     gene            complement(1373..2852)
                     /locus_tag="VFO47_05145"
                     /pseudo
     CDS             complement(1373..2852)
                     /locus_tag="VFO47_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT          374 a         1095 c         1131 g          348 t
ORIGIN      
        1 ccaggatctc caccgggtgg gcgcgcccgc cgatctccag gccgtccttg aacgcgccct
       61 tggcgttctc gacgacgaag ctgtcggcct cgccgaaggc gaacaggggg ccgctctggg
      121 gggagatgta gccgagcctg atcggctggc ggttgccgag gggggacgag gtgccgcagc
      181 cggccagcgc ggccgcgccc gccaaggcgg cgagctgcac gaaccggcgg cggtcgaggt
      241 tgccgttcat gtcaggtcgg cggcggccgg cgtccctgcc aggcgtcctc gagcaggtcc
      301 cggagctcgc tgatcccggc ccgccgcggc gtctggacga ccttggccac ggccagctcg
      361 gccgcccggt cgagctcggc ctcggccagg ccgagctcgg ccaggctggc cggcgtgccc
      421 agtcgccggc ccaggtccca gatcccgccg gcgacgtcgt cgacgccgag gccgccggcc
      481 agcctggcca gctgggggcg ggcggccggg gcgacgaagc ggacggcgtg ggggagcatg
      541 accgcgtgca gctcggcgtg gggcagccgg taggcgccgc cgagcagatg gcagagctgg
      601 tggtggatgc cgaccccggc ggcggccagg gcggccccgg ccagccaggc ccccagcagg
      661 gtgtcggacc gggcggccag gtcggccggg tcggtgacgg cgacgggcag cccgctggcc
      721 aggaccctgg ccccctcggt ggccagcagg gcggtgaccg ggttggcccc gggcgcgtac
      781 agggcctcga cgcagtgggc gagggcgttc atgccgctcg gccccgtcac ctcgggcggc
      841 aggccggtgg tcagggccgg gtcgtagagg acggtgcggg gcaggacccg cgggtcgcgg
      901 ccggtgcgct tgccctcggg gccggtgagc ccgtagatgg gggtcacctc ggagccggcg
      961 taggtggtgg gcacggccac gatcggggcc tcgtgctcca gcgccaccgc cttggccatg
     1021 ccggtggccg acccgccgcc catggcgacc aggcagtcgg cgcccgcctc ggcggccgcg
     1081 gccaccgccc gggcggccgc ctccaccggg acgtgctgct ggacgtcggt gaagctggcc
     1141 gcccagcggc cgccgagccg ttccaccagc ccgtcgacgg cccgcttgcc ggcgatggcc
     1201 agcacccgcc gggccccgag ccggtcgacc tcctcgccca ggcggtcggc cgcgccgacg
     1261 ccgaacacca cccgacccgg cagcgcgtcg taggtgaagg ggggcatggc gcccgactga
     1321 tagccgaacg gttcagggaa gtcaaggcgg cggagacccg ccggatccgg catcagcgcg
     1381 ggtaccagac gggcaggtcc gcgtccacgt tgacccagac cgacttggga tcggtgtagc
     1441 cgtgcatggc ctcgaagccc atctcgcggc cgtagccgga gtcgcccacc ccgccgaacg
     1501 gcgagccggg gctgacccgc ttgtagctgt tgacccagac catgcccgac cggatggccc
     1561 gggccacccg gtgggcccgg gccaggtcgc gggtccagag cccgcccccg aggccgtagt
     1621 cgaccgcgtt ggccagctcc agggcctcgg cctcgccgga gaaggtggtc acggtcacga
     1681 acgggccgaa cacctcctcg cggttgaccc gcgcccccgg gtcggccctg accacggtcg
     1741 gctccacata gcagcccctg gccagcgccg ggtcgtcggg gacgcggccg ccgcacagga
     1801 cctccccgcc ctcgtcgcgg gccaccttca cgtacgccag cacccggtcg cggtgctcgg
     1861 gggaggtgag cggccccatc tcggtcgccg ggtcgagcgg gtcgcccagc cggatgctcc
     1921 gggccagggc caggaaccgc tcctggaact cctcggcgat cggctcctcc aggatcagcc
     1981 gggagccggc gatgcaggcc tggccctggt tgtggaagat cgcgaaggcc gacccgttga
     2041 cggccgcggc cacgtccgcg tcggcgaaga cgatgttggc ccccttgccg cccagctcga
     2101 gctggagccg cttgaggttg ccggccgagg cctcgacgat ccgccggccg acctcggtcg
     2161 agccggtgaa cgacaccttc tgcacgtccg ggtgggcggc cagggcggcc ccggcgacgt
     2221 ggccgtagcc gggcaccacg ttgaccaccc cgggcgggaa gccgacctcg gccatcagct
     2281 cggccacccg cagcgaggtc agcggggtca gctcggccgg cttcagcacc accgtgttgc
     2341 cggcggccag ggccggcccc atcttccagc tgcagaacat cagcgggaag ttccagggga
     2401 cgatctggcc gaccaccccc acgggctcgc ggacgacgta gttgaggaac ccctgctcga
     2461 ccgggatgac cgagccctcg aacttgtcgg ccatgccgcc gaagtagcgg aaggtggcgg
     2521 ccgtgcgggg cacgtccagc cgggacgagt cgcggatggg gtggccggtg tcggccgact
     2581 ccagccgggc cagctcctcg gcccgggcct cgacggcgtc ggccagcttc agcagcagcc
     2641 gtccccgatc ggcggccggg gtggcggccc aggtgggaaa ggccgaccgg gcggccgcca
     2701 ccgcccggtc gacgtccgcc tccctggcct cggccacctc ggccaggtgg ccgccgtcgt
     2761 ggggggctgg tgaccgggat cgtggccccg tcggcggcgt cggtgaactc gcccccgatg
     2821 aacagccggg tcggcccggc cacgccggcc atggcgcccc tagagctcga ccgggtacgt
     2881 cggcagcttg ccgctggaga tgagctcggg caggtcgccg gcggcgttct cccagaccag
     2941 cagcatcg
//
LOCUS       DASSWR010000309         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_577222,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000309 DASSWR010000000
VERSION     DASSWR010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577222"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFO47_05150"
     CDS             <1..355
                     /locus_tag="VFO47_05150"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET8788113.1"
                     /translation="RLDWVAFFAWSPEDGTAALDLDGRVPAAAARDRVEQVQELQDRL
                     LAEAQQAWVGRRLDVLVERVADDGVWEGRSFREAPDSDGVVRVEGAPEVTVGEYLPVM
                     VTAAEGPELLARPAG"
     gene            389..1639
                     /locus_tag="VFO47_05155"
     CDS             389..1639
                     /locus_tag="VFO47_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET8788114.1"
                     /translation="MRCEIIGVGTELLLGQIINPNAAWIGQRLADVGWDCLRHTVVGD
                     NERRIAETVTEALGRADAVILTGGLGPTQDDVTREALAAVAGVKLVRQPELETWLRER
                     FGRMGVQRMAEMNLRQADVPEGARTIDNPRGTAPGLILEIGGKPVYAVPGVPREMEGM
                     LERVVLPDLAARAGEGRAIVSRTLRTAGVGESRLAERLTSLWEEAGAGEVTMAYLASP
                     GEVRVRLTAVGPTREQALGEIAPVETRIREELGDIVYGVDDETLEAAVGRLLRERGLT
                     LATAESLTGGLLGGRITGIAGASDYYLGGVVAYATDAKASQLGVDRDLLDADGPVSEP
                     VAAAMAEGARKAFGADLGLAATGVAGPTEQSGRRVGTLCLGVADPTGTVTQTLTAPGD
                     RTQVRLWTCSVALDLARRRLEGLA"
     gene            1636..2268
                     /gene="thpR"
                     /locus_tag="VFO47_05160"
     CDS             1636..2268
                     /gene="thpR"
                     /locus_tag="VFO47_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET8788115.1"
                     /translation="MSRGDRSRRGSGDPTVTDPDLLTGRLFFAVPVPGAAREPLEAAL
                     PRLAPLLGTARIAPPGGWHLTLAFLGQVRPELADEVVRVGEAAVAGVPTARLRLEGAG
                     TFPAGRRARVLWAGIGGDAEVLVRLAAALSEACKAAGLRSEDRPLMPHLTLARFSTPV
                     PVAQEALDLVAGAAAASPPWEARELACYRSTLGGPRGARYRVVREFPLTG"
BASE COUNT          262 a          898 c          883 g          283 t
ORIGIN      
        1 ccggctcgac tgggtggcgt tcttcgcctg gtcgcccgag gacggcaccg ccgccctcga
       61 cctggacggc cgcgttcccg ccgccgccgc ccgggatcgg gttgagcagg tccaggaact
      121 gcaggaccgt ttgctcgccg aagcccagca ggcctgggtc ggtcggcggc tcgacgtgct
      181 ggtcgagcgg gtcgccgacg acggcgtctg ggagggccgc agcttccggg aggcccccga
      241 cagcgacggc gtggtccggg tcgagggcgc gccagaggtc acggtggggg agtacctgcc
      301 ggtcatggtc accgccgccg agggccctga gcttctggcc cggcccgccg ggtagcatcc
      361 tcgcggtcgt cgatcgcaag gagcgagcgt gcgctgcgag atcatcgggg tcggcaccga
      421 gctgctgctc ggccagatca tcaaccccaa cgccgcctgg atcggccagc gcctggccga
      481 cgtcggctgg gactgcctgc gccacacggt ggtcggggac aacgagcggc gcatcgccga
      541 gacggtcacc gaggcgctcg ggcgggccga cgcggtgatc ctgaccggcg ggctgggccc
      601 gacccaggac gacgtcaccc gggaggcgct ggccgccgtc gccggcgtga agctggtgcg
      661 ccagccggag ctggagacgt ggctgcgcga gcggttcggc cgcatgggcg tccagcggat
      721 ggccgagatg aacctgcgcc aggccgacgt tcccgagggc gcccgcacca tcgacaaccc
      781 gcgggggacg gccccggggc tgatcctcga gatcggtggc aagccggtct atgccgtgcc
      841 cggcgtcccc cgggagatgg agggcatgct ggagcgggtg gtcctgcccg acctggcggc
      901 cagggcgggc gaggggcggg cgatcgtctc caggacgctg cgcaccgccg gggtggggga
      961 gtcgcgcctg gccgagcggc tcacctccct gtgggaggag gccggggccg gcgaggtcac
     1021 catggcctat ctggccagcc ccggtgaggt ccgggtccgg ctcaccgccg tcggcccgac
     1081 gcgtgagcag gccctgggcg agatcgcccc ggtcgagacg aggatccgcg aggagctcgg
     1141 cgacatcgtc tacggcgtcg acgacgagac actggaggcc gccgtcggcc gcctgctccg
     1201 cgagcgcggc ctcaccctgg ccaccgccga gtcgctcacc ggcggcctcc tcggcggccg
     1261 gatcaccggg atcgccggcg cgtccgacta ctacctgggc ggggtggtcg cctacgccac
     1321 cgacgccaag gcgtcccagc tcggcgtcga ccgcgacctg ctggacgccg acggcccggt
     1381 cagcgagccg gtcgccgcgg ccatggccga gggcgcccgc aaggccttcg gcgccgacct
     1441 gggcctcgcc gccaccggcg tcgccggtcc caccgagcag tccggccgcc gggtcggcac
     1501 cctctgcctc ggggtcgccg accccaccgg caccgtcacc cagaccctca ccgcccccgg
     1561 cgaccgcacc caggtccgcc tctggacctg ctcggtcgcc ctcgacctgg cccgccgccg
     1621 cctcgagggc ctggcgtgag ccggggcgac cggtcccggc ggggctcggg cgacccgacc
     1681 gtcaccgacc cggacctgct caccgggcgg ctgttcttcg cggtgccggt gccgggggcg
     1741 gcccgggagc cgctggaggc cgccctgccc cggctggcgc cgctgctcgg caccgcccgg
     1801 atcgccccgc cgggcggctg gcacctcacc ctggcgttcc tcggccaggt gcggccggag
     1861 ctggccgacg aggtcgtccg ggtcggcgag gccgccgtcg ccggcgtccc gaccgcccgg
     1921 ctgcggctgg agggcgccgg cacgttcccc gcggggcgcc gggccagggt gctgtgggcc
     1981 ggcatcgggg gcgacgccga ggtcctggtc cgcctggccg ccgccctctc cgaggcctgc
     2041 aaggccgccg gcctgcgctc cgaggaccgg ccgctgatgc cccacctgac cctggcccgc
     2101 ttctccaccc cggtccccgt cgcccaggag gccctcgacc tcgtcgccgg cgcggccgcc
     2161 gccagcccgc cctgggaggc gcgcgagctg gcctgctacc gcagcaccct cggcggcccc
     2221 cggggcgctc gctaccgggt cgtccgtgag ttcccgctca ccgggtagat cgcaacgacc
     2281 aggacgaacg cggttcgcgg gaagagttgt ccccagttcc ggatat
//
LOCUS       DASSWR010000310         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_887901,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000310 DASSWR010000000
VERSION     DASSWR010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887901"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFO47_05165"
     CDS             <1..857
                     /locus_tag="VFO47_05165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET8788116.1"
                     /translation="PTTCYRHPDRQTGLRCSRCDRPICGECANPAPVGQLCPDDARVT
                     VRTRGLPGTEQPVVTWALLGLNTFMLFAAALLTGSGFGLLEPSARALCRLGALNAAAI
                     AESGQWWRLLTVMVLHGGLLHWAFNSWALWAFGPTLETMLGRLRFLALYVGTGLVGAA
                     ASFAFNQTTLGVGASGAIFGLLGALIAYFFRRRKVGGSMPLQSLLLVLFLNLLIASRS
                     ASIDNLAHIGGFLAGLAAMALYDAAGPRNRGLQVAALAVPYLAGIALAVIGVANYPLG
                     SGLTCVGA"
     gene            complement(838..1563)
                     /locus_tag="VFO47_05170"
     CDS             complement(838..1563)
                     /locus_tag="VFO47_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788117.1"
                     /translation="MFAALLRDAWRQYRRRPLAAVITVVVGGLPVFLEPDNPAVAVPL
                     AVFLLGAGLLVELFLVAWLAGALQEPPPAAAAALATVRGTIGPGVRAGLLRAFYLLAA
                     LMVGLLLFGSREGTGPLPESEQVKLAVGLWPLFGIAFAFLVVLMQRVVLGGERRVRQA
                     ATVSHRVASSHFPICLLIGLLQASGLVLASLVVEFWVLAALSILLALADPYLIGMSNA
                     LYLRTRADQEPAAGDGSLRPPRR"
     gene            1586..>2620
                     /gene="selD"
                     /locus_tag="VFO47_05175"
     CDS             1586..>2620
                     /gene="selD"
                     /locus_tag="VFO47_05175"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET8788118.1"
                     /translation="MRATETPRLTSLSHGAGCACKLPLATLERLMASVGQGLGGSGDG
                     DLLVGAADGDDAAVLRLDDQRALVLTTDFFTPIVDDARDWGRIAAANALSDVYAMGGR
                     PLVALNLTAWPADLPVELLAEVLRGGAEVAGQAGCPVVGGHSIDDPEPKYGLAVVGMA
                     DPGRLLTIARARPGDRLVLTKPLGTGVVATAVKRGDPDPAAVAAAVAAMTTLNNDAAE
                     AAVAAGVEAATDVTGFGLLGHLHRMLRASGVAAEVDAARVPFLPGAAALAGAGFVSGG
                     TRNNEAFLRDHVELEPGLPPVVATLLHDAQTSGGLLLSVAPDILGGLVADLRGRGVEP
                     ALVGRVLAGPA"
BASE COUNT          319 a         1026 c          957 g          318 t
ORIGIN      
        1 ccccgaccac ctgttaccgg catcccgacc ggcagaccgg gctgcgctgc tcgcgctgcg
       61 accggcccat ctgcggcgag tgcgccaacc cggccccggt gggccagctc tgccccgacg
      121 acgcccgcgt cacggtccgg acccgcggcc tccccggcac cgagcagccg gtggtcacct
      181 gggcgctgct cggcctcaac accttcatgc tgttcgcggc cgcgctgctc accggcagcg
      241 gcttcgggct gctggagccg tcggccaggg ccctgtgccg gctgggggcg ctgaacgccg
      301 ccgccatcgc cgagtccggg cagtggtggc ggctgctgac ggtgatggtg ctgcacggcg
      361 ggctgctcca ctgggcgttc aacagctggg ccctgtgggc gttcgggccg accctggaga
      421 ccatgctcgg ccggctccgc ttcctcgccc tgtacgtcgg caccggcctg gtcggggccg
      481 cggccagctt cgccttcaac cagaccaccc tgggggtcgg cgcctcgggg gccatcttcg
      541 gcctgctcgg cgccctgatc gcctacttct tccggcgccg caaggtcggc ggctcgatgc
      601 cgctccagag cctcctgctg gtgctgttcc tcaacctgct gatcgcctcc cgcagcgcca
      661 gcatcgacaa cctggcccac atcggcgggt tcctggccgg cctggccgcc atggccctgt
      721 acgacgcggc cgggccgcgc aaccgcggcc tccaggtggc cgcgctggcc gtgccctacc
      781 tggccgggat cgccctggcc gtcatcgggg tggccaacta ccccctcggc agcggcctca
      841 cctgcgtggg ggcctgagcg acccgtcgcc ggccgccggc tcctggtcgg cccgggtccg
      901 gaggtagagg gcgttggaca tgccgatcag gtaggggtcg gccagggcga gcaggatcga
      961 cagcgcggcc aggacccaga actcgaccac caggctggcc agcaccagcc cgctcgcctg
     1021 gagcaggccg atgagcaggc agatggggaa gtgggatgac gccacccggt gggagacggt
     1081 cgcggcctgc cggacccggc gctcgccgcc gagcacgacc cgctgcatga gcacgaccag
     1141 gaaggcgaac gcgatcccga acagcggcca caggccgacc gccagcttga cctgctcgga
     1201 ctcgggcagc ggcccggtgc cctcgcggga gccgaacagc agcagcccga ccatcagcgc
     1261 cgccagcagg tagaaggcgc gcagcagccc ggcccggacg ccggggccga tggtgccgcg
     1321 cacggtggcc agggcggccg cggccgccgg cggcggctcc tggagggcgc cggccagcca
     1381 cgccaccagg aacagctcga ccagcagccc cgcgcccagc aggaacacgg ccaggggcac
     1441 ggccaccgcc gggttgtcgg gctccaggaa cacgggcagc ccgcccacga ccaccgtgat
     1501 cacggcggcc agcgggcggc gccggtactg gcgccaggcg tcgcgcagaa gggcggcgaa
     1561 catgggcgag gcctatccta cggggatgcg tgctacagaa accccccgtc tgacctctct
     1621 ctcccatggg gcgggctgcg cctgcaagct gcccctggcc acccttgagc ggctcatggc
     1681 cagcgtcggc caggggctgg gcgggagcgg cgacggcgac ctgctggtcg gggcggccga
     1741 cggcgacgac gcggccgtgc tgcgcctcga cgaccagcgg gcgctggtcc tcacgaccga
     1801 cttcttcacc ccgatcgtcg acgacgcccg cgactggggg cggatcgccg ccgccaacgc
     1861 cctctcggac gtctacgcca tggggggccg gcccctggtc gccctcaacc tgaccgcctg
     1921 gccggccgac ctgccggtcg agctgctggc cgaggtgctg cggggcggtg ccgaggtggc
     1981 cggccaggcc ggctgcccgg tcgttggcgg ccactcgatc gacgaccccg agcccaagta
     2041 cggcctggcc gtggtcggca tggccgaccc gggccggctg ctgaccatcg cccgggcccg
     2101 gccgggggac cggctggtgc tgaccaagcc gctcggcacc ggggtggtcg cgaccgcggt
     2161 caagcgcggc gaccccgacc cggccgccgt cgcggccgcg gtcgccgcca tgaccaccct
     2221 gaacaacgac gccgccgagg ccgccgtggc cgccggggtc gaggccgcca ccgacgtgac
     2281 cggcttcggg ctgctcggcc acctccaccg gatgctgcgg gccagcggcg tggccgccga
     2341 ggtcgacgcc gcccgggtgc cgttcctgcc cggcgcggcc gcgctggccg gagccggctt
     2401 cgtctccggc ggcacccgca acaacgaggc cttcctgcgc gaccacgtcg agctggagcc
     2461 gggcctgccc ccggtggtgg ccaccctgct ccacgacgcc cagacctcgg gcggcctgct
     2521 gctgtcggtg gcccccgata tcctcggcgg gctggtcgcc gacctgcgcg gccgcggcgt
     2581 ggagccggcc ctggtcggcc gcgtgctcgc cggcccggcc
//
LOCUS       DASSWR010000311         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_605216,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000311 DASSWR010000000
VERSION     DASSWR010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605216"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFO47_05180"
     CDS             complement(<1..232)
                     /locus_tag="VFO47_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020217433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788119.1"
                     /translation="MRLPVAGDARVFLTRLVRLDAAALVRLRPDASGTRTALWARLPW
                     GVLVTRTVDGAVDGDRTVSAAALLAAEDGWPPA"
     gene            complement(243..1919)
                     /gene="ettA"
                     /locus_tag="VFO47_05185"
     CDS             complement(243..1919)
                     /gene="ettA"
                     /locus_tag="VFO47_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET8788120.1"
                     /translation="MAQFIYVLDRARKAHGDKVVLDNVTLSFLPGAKIGVVGPNGAGK
                     SSLLRIMAGLDRPSNGEARLMPGYTVGLLAQEPPLTEDKTVRENIEEAVADVKAKLAR
                     FNEVAEKLAVDYSDELMAEMGKLQEELDHADAWDLDSKLELAMDALRCPPGDTPVSVL
                     SGGERRRVALCKLLLQQPDLLLLDEPTNHLDAESVQWLEQHLAKYPGTVLAVTHDRYF
                     LDHVAEWILELDRGRAYPYEGNYSTYLEKKAERLQVQGRKDAKLRRRLAEELEWVRSN
                     ARARQAKSRARLQRYEEMAAEAEKHRKLDFEEIQIPPGPRLGNQVIEVQDLVKGFDGR
                     ILIDRLSFSLPRNGIVGIIGPNGVGKTTLFKTIVGLEKPDSGTVRIGETVKLSYVDQT
                     RSGLDPNKTVWEVVSDGLDHLMVGKVEMPSRAYVAAFGFKGPDQQKPAGVLSGGERNR
                     LNLALTLKIGGNVILLDEPTNDLDVETLSSLENALLDFPGCAVVISHDRMFLDRVATH
                     ILAWEGDEENPAKWFWFEGNFESYLKNKVERLGEEAARPHRVTHRRLTRD"
BASE COUNT          307 a          861 c          778 g          372 t
ORIGIN      
        1 gcgccggcgg ccacccgtcc tcggccgcca gcagtgcggc ggccgacacg gtccggtcgc
       61 cgtccaccgc gccgtcgacc gtgcgggtca ccagcacccc ccacggcagc cgtgcccaca
      121 gcgcggtccg cgtccccgag gcgtccggcc gcaaccggac cagtgccgcc gcgtccagcc
      181 ggaccagccg ggtgaggaac acccgggcgt cgcccgccac cgggagccgc atggtcgtcc
      241 ggtcagtcgc gggtgagccg tcggtgggtc acccggtgcg gacgggccgc ctcctcaccc
      301 agccgctcga ccttgttctt gaggtacgac tcgaagttgc cctcgaacca gaaccacttg
      361 gccgggttct cctcgtcgcc ctcccaggcg aggatgtggg tcgccacccg gtcgaggaac
      421 atccggtcgt gggagatcac caccgcgcag ccggggaagt ccagcagggc gttctccagg
      481 ctggacaggg tctccacgtc caggtcgttg gtgggctcgt cgagcaggat cacgttcccg
      541 ccgatcttga gggtgagggc gaggttgagc cggttgcgct cgccgccgct gagcaccccg
      601 gccggcttct gctggtccgg gcccttgaac ccgaacgccg ccacgtacgc ccgcgacggc
      661 atctcgacct tgccgaccat caggtggtcc agcccgtcgc tgacgacctc ccagacggtc
      721 ttgttcgggt ccaggcccga gcgggtctgg tcgacgtacg acaacttgac cgtctcgccg
      781 atgcgtaccg tgccggagtc cggcttctcc aggccgacga tggtcttgaa cagggtggtc
      841 ttgcccaccc cgttggggcc gatgatcccg acgatgccgt tgcgcggcag cgagaagctg
      901 agccggtcga tgaggatgcg gccgtcgaaa cccttgacca ggtcctgcac ctcgatgacc
      961 tggttgccca gccgcggccc cggcggaatc tggatctcct cgaaatccag cttgcggtgc
     1021 ttctccgcct cggccgccat ctcctcgtac cgctgcagcc gggcacggct cttggcctgc
     1081 cgggcgcggg cgttggagcg tacccactcc agctcctcgg ccagccgcct gcgcagcttg
     1141 gcgtccttgc ggccctgcac ctgaaggcgc tcggccttct tctccaggta ggtggagtag
     1201 ttgccctcgt acgggtaggc ccggccgcgg tccagctcca ggatccactc ggcgacgtgg
     1261 tcgaggaagt agcggtcgtg ggtgaccgcc agcaccgtgc ccgggtactt ggccaggtgc
     1321 tgctccagcc actgcacgct ctcggcgtcc aggtggttgg tgggctcgtc cagcagcagc
     1381 aggtccggct gctgcagcag cagcttgcac agcgccaccc ggcgccgctc acccccggac
     1441 agcacggaca ccggggtgtc gccgggcggg cagcgcagcg cgtccatggc cagctccagc
     1501 ttggagtcca ggtcccacgc gtcggcgtgg tccagctcct cctgcagctt gcccatctcg
     1561 gccatcagct cgtcggagta gtcgaccgcc agcttctcgg cgacctcgtt gaaccgggcg
     1621 agcttggcct tgacgtcggc gaccgcctcc tcgatgttct cccggacggt cttgtcctcg
     1681 gtcagcggcg gctcctgcgc cagcaggccc acggtgtaac cgggcatgag cctggcctca
     1741 ccgttgctcg gacggtccag cccggccatg atccgcagca ggctcgactt gccggcgccg
     1801 ttcgggccga ccacaccgat cttggctccc ggcaggaagc tcaaggtcac gttgtcgagc
     1861 acgaccttgt cgccgtgggc cttgcgggcc cggtcgagca cgtagatgaa ctgggccact
     1921 gggcggtgta cctcccccga acctgtcgtc cgcgtacctg tccatcttcc cagtggcgct
     1981 ccggcgccgc ccagccaacc ccggatcacg gcgcgtcccg ggggtcggtg cacgcgtaca
     2041 cccggcgcgg cggacccgcg cgaccggagg gtgacccggc cggccgctac cgtggagggc
     2101 gccacggtga cccgcgctga tcgtcgaccg cggccgtgtg agcggcggca gaacggcgag
     2161 gtgtgcgacc gacaccgatc ctttgtgcca tgactgccac accgggcgag gcgccgggga
     2221 cgggttcttc ttccttcggc gagccgccgg cgtcggtacg gccgtccacc gccgaacgcc
     2281 aggccgtcgc cgcgcaactc ctcgccgccg tcaacgac
//
LOCUS       DASSWR010000312         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_435929,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000312 DASSWR010000000
VERSION     DASSWR010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435929"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFO47_05190"
     CDS             complement(<1..287)
                     /locus_tag="VFO47_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788121.1"
                     /translation="MSPGRRRRLARSALLLVLVLAAGALWLRDRLEVAPATAPGPAVA
                     ARPAPAADPGAAAADAEGRPEGRTWRLRVTSHPPGARLSIRPAAGAPRQART"
     gene            443..1876
                     /locus_tag="VFO47_05195"
     CDS             443..1876
                     /locus_tag="VFO47_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET8788122.1"
                     /translation="MADARVLALDLGTSSVRALVFDDRGVAVPGVLARRPTRLDLTDE
                     GKAELDPDEVAAALGECLDELAARGELDGVQHVATSCAWHSVIALDAGGGRLTGALTW
                     ADTRAAPLVAELRSRGDMDRLHAATGARPHTLYWTVKLPWLARVLSPAPARYLGLAEY
                     ALGALLDDPTVSRSMASGTGLLDLASAAWDAQALALAGVDERALPAVAGPGWTGRLGP
                     EGARRWPALAEATWHPVTGDGAASNVGAGCVSPDLVAINIGTSAAIRAVEPADQARPL
                     HRGLWRYLVDDDHVVSGAAFSGAGNLYAWLRQVTTLPAEGSVEDELATVPPGSRGVVV
                     MPYHAGSRPPLDLAAGSGAITGLSLATTPVEILAATLESVCYRLAAGYEALAATLPKP
                     PAVVASGGAIVASPWWQQTLADVLGRPVRVVDEPEASARGAALLALGLTTDPATVRVV
                     DPRPDAVEAQRAARALHEDLATRLGYV"
     gene            1907..2095
                     /locus_tag="VFO47_05200"
     CDS             1907..2095
                     /locus_tag="VFO47_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788123.1"
                     /translation="MSEYEQEQELVEQLPEDIDPHEMADSVEDELVAEEGVELGLDPD
                     LALFAARDELEEGEEPPL"
     gene            2113..>2612
                     /locus_tag="VFO47_05205"
     CDS             2113..>2612
                     /locus_tag="VFO47_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET8788124.1"
                     /translation="MEQRALITGIGTVNPLGLDAPSTWEAMLAGRSGIETIAAFDPSP
                     YPVRIAGEVRGFDPETVASRKDARRMDRGVLFAVAAADEAVADSGIDKLDPERTGVVV
                     GSAIGGIGIVEEQQRILLEKGPGRVSATFLPNCLVDTATSYIATRLGVVGLNFAVVSA
                     CATGSHA"
BASE COUNT          303 a         1004 c         1003 g          302 t
ORIGIN      
        1 gtccgggcct gccggggggc gccggcggcg ggccggatgg agagccgggc gccgggcggg
       61 tggctggtga cccgcaggcg ccaggtccgg ccctcgggcc ggccctcggc gtcggcggcc
      121 gccgcacccg ggtcggcggc cggggccggg cgggccgcca ccgccggacc tggggcggtc
      181 gccggcgcga cctcgagccg gtcacggagc cagagggcgc ccgccgccag caccaggacg
      241 aggagcaggg cgctcctggc caggcgccgg cgccgacccg gcgacatccg ttgcatctcc
      301 gctgttccac ggttggcggc cgaacgcgac ctaccatagc gcgccggcac gaccgggagg
      361 gggacgccgg gggcggtagg aacgggagcg gctgggtacc aaggggagac tgacgcggat
      421 gggccggggt cgaggaggcg gcgtggcgga cgcgcgggtg ctggcgctgg acctgggcac
      481 gtcgtcggtg cgggcgctgg tcttcgacga ccgcggggtc gcggtgcccg gcgtcctggc
      541 ccgccggccc acccggctgg acctcaccga cgagggcaag gccgagctcg accccgacga
      601 ggtggccgcc gccctcggcg agtgcctcga cgagctggcc gcccgcgggg agctggacgg
      661 cgtccagcac gtggccacct cctgcgcctg gcattcggtg atcgccctgg acgccggtgg
      721 cggccgcctg accggggcgc tcacctgggc cgacacccgg gccgcgccgc tcgtggccga
      781 gctgcggtcc cgcggcgaca tggaccgcct gcacgccgcc accggcgccc gcccccacac
      841 gctgtactgg accgtgaagc tgccctggct ggcccgggtg ctctcccccg ccccggcccg
      901 ctacctgggg ctggccgagt acgccctcgg ggccctgctg gacgacccga ccgtctcccg
      961 gtcgatggcc tcggggacgg ggctgctgga cctggccagc gccgcctggg acgcccaggc
     1021 gctggccctg gccggcgtcg acgagcgggc cctgcccgcc gtggccggcc ccggctggac
     1081 gggccggctg gggccggagg gggcgcgccg ctggccggcc ctggccgagg cgacctggca
     1141 cccggtgacc ggcgacgggg ccgcctccaa cgtcggcgcc ggctgcgtct cgcccgacct
     1201 ggtggccatc aacatcggca cctcggccgc catccgggcc gtcgagccgg ccgaccaggc
     1261 ccgccccctc caccgcgggc tctggcgcta cctggtcgac gacgaccacg tggtcagcgg
     1321 ggccgcgttc tcgggggccg ggaacctgta cgcctggctg cgccaggtca ccaccctgcc
     1381 ggccgagggc tcggtcgagg acgagctggc cacggtcccg cccgggtcgc ggggggtggt
     1441 ggtgatgccg taccacgccg ggtcccgccc gccgctcgac ctggccgccg ggtcgggggc
     1501 gatcaccggc ctgtcgctgg ccaccacccc ggtcgagatc ctggccgcca ccctggagtc
     1561 ggtctgctac cggctggccg ccggctacga ggccctggcc gccaccctgc ccaagccgcc
     1621 ggcggtggtc gcctccggcg gggcgatcgt cgcctccccc tggtggcagc agaccctggc
     1681 cgacgtgctc ggccgcccgg tccgggtggt cgacgagccc gaggcgtcgg ccaggggggc
     1741 ggccctgctc gccctcggcc tgaccaccga cccggccacc gtccgcgtgg tcgacccccg
     1801 ccccgacgcc gtcgaggccc agcgggccgc ccgggccctg cacgaggacc tggccacccg
     1861 tctgggttac gtttgaccgc ccatccgacc aaggagccaa ccaccggtga gtgagtacga
     1921 gcaggaacag gagctggtcg agcagctgcc cgaggacatc gacccccacg agatggccga
     1981 cagcgtcgag gacgagctgg tcgccgagga gggcgtggaa ctggggctcg accccgacct
     2041 ggccctgttc gcggccaggg acgaactgga agagggcgag gagccgcccc tgtaggctac
     2101 gctctgttcc acatggaaca gcgcgcgctc atcaccggca tcggcaccgt caacccgctc
     2161 gggttggacg ccccctccac ctgggaggcg atgctggccg ggcgcagcgg catcgagacc
     2221 atcgccgcct tcgacccgtc gccctacccg gtccggatcg ccggcgaggt ccgcggcttc
     2281 gaccccgaga cggtggcctc gcgcaaggac gcccggcgca tggaccgcgg ggtgctgttc
     2341 gcggtggccg cggccgacga ggcggtggcc gactccggca tcgacaagct cgacccggag
     2401 cgcaccgggg tggtcgtcgg ctcggccatc gggggcatcg ggatcgtcga ggagcagcag
     2461 cgcatcctgc tcgagaaggg cccgggccgg gtctcggcaa cgttcctgcc caactgcctg
     2521 gtcgacaccg ccaccagcta catcgccacc aggctggggg tggtcgggct caacttcgcg
     2581 gtcgtgtcgg cctgcgccac cggctcccac gc
//
LOCUS       DASSWR010000313         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_605268,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000313 DASSWR010000000
VERSION     DASSWR010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605268"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFO47_05210"
     CDS             <1..897
                     /locus_tag="VFO47_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8788125.1"
                     /translation="SITSASSVGPPELGRLVETRRPIVWGGRGMRQVEAAPRGVLDPG
                     AGARRFEVGRSRPCPDLAELVEHFWTVRWDLRGRGPHTQHTLPHPSVHLVAEPGRSGI
                     MGVLTGRFTRELEGAGRAFGIRFRPAGFHPFLGAPVASLTDRRVAVAAVFGPDGDRLV
                     GDLLAAAGEPELVATAEAFLLDRLPAPDPNVALVNRAVDLIMADQDLTRVGDAAERAG
                     VGTRRLQRLFAAYVGVTPKWVIRRSRLHEAVERLDRGDHVDLGFLARDLGYFDQAHFA
                     RDFRAAVGRPPAAYARGSASPG"
     gene            complement(904..1671)
                     /locus_tag="VFO47_05215"
     CDS             complement(904..1671)
                     /locus_tag="VFO47_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8788126.1"
                     /translation="MDNQRHQGRVAVVTGAGSGIGRAVAARLAAEGAAVVGCDVNQAG
                     LEETLGAVREGGNDATVVMTDVTSQDQVDRLVDDAVGRHGRVDLLANVAGIMDWFLPA
                     HEVDDETWNRVMAVNVNGPMMLCRKVLPGMLERGGGAIVNVASVAGLRGGGAGIAYTT
                     SKHALVGMTRSIAWTYRADGIRCNAICPGGVQTNIGTTAVPRSQFAFERLGKSLALAE
                     KAADPDEIAALASWLASDEASNVNGAIVTADAGWTAG"
     gene            complement(1934..2599)
                     /locus_tag="VFO47_05220"
     CDS             complement(1934..2599)
                     /locus_tag="VFO47_05220"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HET8788127.1"
                     /translation="MELLPAIVGLAVVDSINPSALLATIALLLPGRPARPLVAVYVVA
                     VLVTYLAIGLALTLGLGLTPQAVLESDAAYLAQGVLGAAMLAYAVAAPGRRRERGPAR
                     PRRLPAAGRPAAVFALGVAVTALELPTALPYLGAVGAITRAELAVAEWLPLLLLYNLI
                     FVLPPLALLAAHLALGDRADRLLERLRDHLGGGAREGLLWLLGLVGFFLLADALGHFE
                     VLA"
     gene            complement(2606..2806)
                     /locus_tag="VFO47_05225"
     CDS             complement(2606..2806)
                     /locus_tag="VFO47_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788128.1"
                     /translation="MQARAQRPARWAPWWLYVIAIVVTNQVRMQWLQPDDLATWVEVV
                     LGVGAMALVAAVVTAVWRAVRR"
     gene            2880..>3321
                     /locus_tag="VFO47_05230"
     CDS             2880..>3321
                     /locus_tag="VFO47_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET8788129.1"
                     /translation="MPKLVDPEQRRAAIAEAVWTVIRRDGLQRASVRNVAREAGLSMG
                     SLRHYFSTQSELLCFAMQLVGDRARARVRTLRPAADPRQGAEQLLAELVPLDDERLAE
                     SEVWLAFTDRALVDPDQRAIHQRIHDQLYGACVTAVTLLADAGLT"
BASE COUNT          426 a         1298 c         1183 g          414 t
ORIGIN      
        1 tcgatcacct ccgcgtccag tgtcggcccg ccggagctgg gacggcttgt agaaacgcga
       61 cggccgatcg tctggggagg tcgagggatg cggcaggtcg aggcggcgcc acggggcgtg
      121 ctcgacccgg gcgccggggc ccggcgcttc gaggtcgggc gctcccgccc gtgccccgac
      181 ctggccgagc tggtcgagca cttctggacg gtgcgctggg acctccgggg ccgcggcccc
      241 cacacccagc acacccttcc ccacccgtcg gtgcacctgg tcgccgagcc gggccgctcg
      301 gggatcatgg gcgtgctcac cggccgcttc acccgcgagc tggagggcgc gggccgcgcc
      361 ttcgggatcc ggttccggcc ggccggcttc cacccgttcc ttggcgcccc cgtcgcgtcc
      421 ctcaccgacc gccgggtcgc cgtcgccgcc gtgttcgggc ccgacggcga ccggctggtc
      481 ggcgacctgc tggccgccgc cggcgagccc gagctggtcg ccaccgccga ggcgttcctc
      541 ctcgaccggc tgcccgcccc ggacccgaac gtggccctgg tcaaccgcgc cgtcgacctg
      601 atcatggccg accaggacct gacccgggtc ggggacgcgg ccgagcgggc cggcgtcggc
      661 acccgccggc tccagcgcct gttcgccgcc tacgtcggcg tcacccccaa gtgggtcatc
      721 cggcgctccc gcctccacga ggcggtcgag cgcctcgacc ggggcgacca cgtggacctg
      781 gggttcctgg cccgcgacct cggctacttc gaccaggccc atttcgccag ggacttccgc
      841 gccgccgtcg gccgcccgcc cgccgcctac gcccgcgggt cggccagccc cggctgagcc
      901 cggctacccg gcggtccagc cggcgtcggc ggtgacgatg gcgccgttga cgttggaggc
      961 ctcgtcggag gcgagccagg aggccagggc cgcgatctcg tcggggtcgg ccgccttctc
     1021 ggccagggcc aggctcttgc ccaggcgctc gaaggcgaac tggctgcggg gcaccgccgt
     1081 ggtgccgatg ttggtctgga ccccgccggg gcagatcgcg ttgcagcgga tgccgtcggc
     1141 ccggtaggtc caggcgatcg aacgggtcat cccgaccagc gcatgcttgg aggtcgtgta
     1201 ggcgatcccg gccccgccgc cgcggagccc ggcgaccgag gccacgttga cgatcgcgcc
     1261 cccgccccgc tccagcatgc ccggcaggac cttgcggcag agcatcatcg gcccgttgac
     1321 gttgaccgcc atcacccggt tccaggtctc gtcgtcgacc tcgtgcgccg ggaggaacca
     1381 gtccatgatc ccggccacgt tggcgagcag gtcgacccgg ccgtggcgcc cgaccgcgtc
     1441 gtcgaccagc cggtcgacct ggtcctggct ggtgacgtcg gtcatgacca cggtggcgtc
     1501 gttcccgccc tcccggaccg cgcccagcgt ctcctccagc ccggcctggt tgacgtcgca
     1561 cccgaccacg gccgcgccct ccgcggccag ccgcgccgcc accgcccgcc cgatcccgga
     1621 cccggccccg gtgacgaccg ccacccgtcc ctggtgccgt tggttgtcca tgcccgcctc
     1681 ccgcgttctt ggtcccgagg tggtggcccg cgtgtccacc tcccgccagg ccagcacacg
     1741 aagcggatct tccgaccggg ccggggggca cggcaaccgc gaacacggtc gtctgctcgt
     1801 acgtaactcg gccgacgggc gcggccggca gcagcagcag gccgcgcacg atcgggttgc
     1861 tcgacggccg cccattcgcc gcctaccgtc caacggtcgc caatgcccga aatcgagcat
     1921 ctcgcggtgg tggtcaggcc aggacctcga agtggcccag ggcgtcggcg agcaggaaga
     1981 acccgaccag gccgaggagc cacagcaacc cctccctggc gcccccgccg aggtggtcgc
     2041 gcagccgctc cagcagccgg tcggcccggt ccccgagggc caggtgcgcg gccagcaggg
     2101 ccagcggggg gagcacgaag atcaggttgt agagcagcag cagcggcagc cattcggcaa
     2161 cggccagctc ggcgcgggtg atcgccccca ccgcgcccag gtaggggagg gcggtgggca
     2221 gctcgagggc ggtgacggcc accccgaggg cgaacaccgc cgcgggtcgc ccggccgcgg
     2281 gcagccgccg gggccgggcc gggccgcgct cgcgccgccg gccgggggcg gccacggcgt
     2341 aggccagcat ggccgccccg agcacgccct gggccaggta ggcggcgtcg ctctcgagca
     2401 ccgcctgcgg cgtgagcccc aggccgaggg tgagggccag gccgatggcc aggtaggtga
     2461 ccagcaccgc gaccacgtac acggcgacca gcggccgggc gggccggcca gggagcagga
     2521 gcgcgatcgt ggccagcagg gccgacgggt tgatgctgtc gaccaccgcc aggccgacga
     2581 tcgcggggag cagctccatc ggtggctagc gccggaccgc ccgccagacg gcggtgacga
     2641 cggccgcgac cagggccatg gcccccaccc cgagaacgac ctcgacccag gtcgccaggt
     2701 catcgggctg gagccactgc atgcggacct ggttggtgac gacgatcgcg atgacgtaga
     2761 gccaccaggg cgcccaacgg gccgggcgct gcgctcgtgc ctgcatggtc tcgcacctcc
     2821 tcgatctagc acagttgtgc gataaccaca tggtagcacg gttgtgctat aaaggtggca
     2881 tgccgaagct ggtcgatccc gaacagcgcc gggcggcgat cgccgaggcg gtgtggacgg
     2941 tcatccgccg cgacgggctc cagcgggcct ccgtccgcaa cgtcgcccgc gaggccggcc
     3001 tgtccatggg ctcgctgcgc cactacttct cgacccagtc cgagctgctg tgcttcgcca
     3061 tgcagctggt gggcgaccgc gcccgggccc gcgtccgcac cctgcgcccg gccgccgacc
     3121 cgcggcaggg cgccgagcag ctcctggccg agctcgtccc cctcgacgac gagcgactgg
     3181 ccgagagcga ggtctggctg gccttcaccg accgcgccct cgtcgacccc gaccagcgcg
     3241 ccatccacca gcgcatccac gaccagctct acggcgcctg cgtcaccgcc gtcaccctcc
     3301 tggccgacgc cggcctgacc c
//
LOCUS       DASSWR010000314         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1029529, whole genome shotgun sequence.
ACCESSION   DASSWR010000314 DASSWR010000000
VERSION     DASSWR010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029529"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFO47_05235"
     CDS             <1..831
                     /locus_tag="VFO47_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788130.1"
                     /translation="HLMNLLWGVVIVAVTVAVAVAAMLLVRRRAPEGSFFSDGDRASG
                     VFGVLATGFALLLGLIVFLAFTSYDDSKKGAESEALALVQQFETAQFLPLAASGRLGG
                     ELVCYGRSVVGQEWPRMETGTESGAINPWAIALFRTMKTVQPASATEQSAYDKWFDLT
                     STREEARRDRVHASEGVIPTPLWWVLFFTAGLIVVYMLFFADSGERAVVQGLLMGSVV
                     AVMVATLLLIRTLDAPYRPGLGQLRPVAMEQTLGLIEQARTVLGDDAPLPCDAEGRPR
                     "
     gene            828..1460
                     /locus_tag="VFO47_05240"
     CDS             828..1460
                     /locus_tag="VFO47_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788131.1"
                     /translation="MTGRRLEAWLEVVSAVVLSVAALAIAWSGYQASRWTGEQAKAFS
                     RANAARVESTRWSDLANSQSEIDVATFIQWVDSYARDETELADFYRKRFREEFRPAVE
                     AWIATRPLHNPEAPLTPFATPAYHLAARAEAERLEARAGAESAVASRNIQRATNYVLC
                     VVLFSAALFFGGISTRIKSPGTRAVVLGLGCLVLLGALAWLTTFPISISV"
     gene            1471..1950
                     /locus_tag="VFO47_05245"
     CDS             1471..1950
                     /locus_tag="VFO47_05245"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HET8788132.1"
                     /translation="MLIVVRHAEAGSKGSWAGPDLHRPLSPEGHDQAEGLVVRLDDYP
                     VERILSSPAARCLQTVEPLGRDRLLQVEEVAALGADADAARVESLFVGPEAAHVVLCT
                     HGELIGRLFARLVPAGLVVEDPLLWPKGSAWLLWRTHRRLHARFLTPLALDRRTYAG"
     gene            complement(1955..2179)
                     /locus_tag="VFO47_05250"
     CDS             complement(1955..2179)
                     /locus_tag="VFO47_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788133.1"
                     /translation="MPSKSANVKNEKQYEALKDKGMSKERAAKIANSPGASKRGGKAS
                     GKGGSAKQGGTTAQKKAAGRKGGKAAAKKS"
BASE COUNT          284 a          856 c          774 g          383 t
ORIGIN      
        1 catctaatga atcttctctg gggtgttgtc atcgtggcgg tgacggtggc ggtggccgtg
       61 gcggccatgt tgctggttcg ccggcgtgcc ccggagggca gcttcttcag cgacggtgac
      121 cgcgcctctg gggtgttcgg agtgctcgcc accgggttcg ccctcctgct cgggctcatc
      181 gtgttcctcg ccttcaccag ctacgacgac tccaagaagg gcgccgagag cgaggccctg
      241 gccctggtcc agcagttcga gacggcccag ttcctgccgc tcgccgccag cgggaggctc
      301 ggcggggagc tggtctgcta cggacggtcg gtggtgggcc aggagtggcc gcggatggag
      361 accggcaccg agagcggggc gatcaacccc tgggctatcg ccctgttcag gaccatgaag
      421 accgtccagc cggcaagcgc gaccgaacag tccgcctacg acaagtggtt cgacctgacc
      481 tccacgcgcg aggaggcccg gcgcgaccgc gtccacgcct ccgaaggcgt catcccgaca
      541 ccgctgtggt gggtcctgtt cttcaccgcc gggctcatcg tggtctacat gctgttcttc
      601 gccgacagcg gggagcgggc ggtcgtccag ggcctgctga tgggctcggt cgtggccgtc
      661 atggtcgcga ccctgctgct gatcaggacc ctggacgcgc cctaccggcc tggcctcggc
      721 cagcttcggc cggtcgccat ggagcagacc ctcgggctga tcgagcaggc ccgcacggtc
      781 ctcggggacg acgcccccct cccctgcgac gccgagggac gaccacggtg accggccggc
      841 ggctggaggc ctggctcgag gtcgtctcgg ccgtggtcct ctccgtggcc gcgctggcca
      901 tcgcctggag cggctaccag gcgagccgct ggacgggcga gcaggccaag gccttcagcc
      961 gggccaacgc cgcccgggtg gagtcgaccc gctggtcgga cctggccaac agccagagcg
     1021 agatcgacgt cgccaccttc atccagtggg tcgacagcta cgcccgcgac gagaccgagc
     1081 tggccgactt ctaccgcaag cggttccgcg aggagttccg gccggccgtc gaggcctgga
     1141 tcgccaccag accgctgcac aaccccgagg cccccctgac gcccttcgcc acccccgcct
     1201 accacctggc cgccagggcc gaggccgagc gccttgaggc gcgggcgggg gcggagtccg
     1261 ccgtggccag ccggaacatc cagcgcgcca ccaactacgt cctgtgcgtc gtcctgttct
     1321 cggccgcgct gttcttcggc ggcatcagca cccgcatcaa gagcccgggc acgcgcgccg
     1381 tcgtcctcgg gctcggctgc ctggtgctgc tcggcgccct ggcctggctc accacgttcc
     1441 cgatcagcat ctcggtgtag ggggtaggac atgctcatcg tcgtccggca cgccgaggca
     1501 gggagcaagg gaagctgggc cggccccgac ctgcatcggc ccctctcccc cgaaggccac
     1561 gaccaggccg aggggctggt cgtccggctg gacgactacc cggtcgagcg gatcctgtcc
     1621 agcccggccg cgcgctgcct ccagaccgtc gagccgctcg ggcgcgaccg cctgctccag
     1681 gtcgaggagg tcgcggccct cggcgccgac gccgacgcgg cccgggtgga gtcgctcttc
     1741 gtgggcccgg aggccgccca cgtggtgctg tgcacccacg gcgagctgat cgggcgattg
     1801 ttcgcccggc tcgtcccggc cggcctggtc gtcgaggacc cgctgctctg gcccaagggc
     1861 tcggcctggc tgctgtggcg gacccatcgc cggctgcacg cccgcttcct gaccccgctg
     1921 gccctcgacc ggcgcaccta cgccggctga ccgctcagct cttcttcgcg gccgccttcc
     1981 cgcccttgcg cccggccgcc ttcttctggg ccgtcgtccc gccctgcttg gcgctcccgc
     2041 ccttgccgga ggctttcccg ccccgcttgg acgcccccgg cgaattggcg atcttggccg
     2101 cgcgctcctt ggacatcccc ttgtccttga gcgcctcgta ctgcttctcg ttcttgacgt
     2161 tcgccgattt ggacggcatt tcaccctccg tcgtgccggg attcctccag ccgaggttcc
     2221 cggaacctca ccggcctaat cgtgcgataa ctgatgtacg gttaggcctg attttcgagc
     2281 aggagtcgac cgcgcct
//
LOCUS       DASSWR010000315         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_775271,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000315 DASSWR010000000
VERSION     DASSWR010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775271"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2509
                     /locus_tag="VFO47_05255"
     CDS             <1..>2509
                     /locus_tag="VFO47_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788134.1"
                     /translation="ALLKAPIRLVHFPGVAVAVVAAALILALTATSGRLFLASAGDAA
                     VGQELDRIGGVPMLAMVMFGDRPADVAAVQRAAESVASDLVPGLGPPVRTRSGATMDA
                     AAGTRTARVRLAARDGFAGHIQALERAGGDGAWLPDTAASSLGVRAGQTVRLGLPPGV
                     RVRVVGVYRDLATSDQPLDRFWSPLGNVIYTLDQEERTTPPGLVLVDPDRFLELTRRL
                     ESPGQLEWDFYPESDRLTLPEADRLAAQIQAVQGTTGDPWAEQAGLTRVTTATPITDV
                     VRRAHATLAALTGPVESISLTGRLLALALVAAAAVFAVRRRRGEVSVLIAHGVGGLPI
                     GARSMVEAALPLAAGGALGYLVALGLAGPLDPVPGLQPQATAAARREVALAMAAGLLL
                     FGLVTWIAVRSEERERARRLGQVLATPWWEVLALALAAAALYELRTRGGAPVQVEGEP
                     AQVDRLLVLFPLLAIAGLAGLATRAAARLLRRARPPLTAASAARPAPVLALRRLVAAP
                     RTVLLLVTGSAVALGLLTYSGTLVASTRSGAVDKARVLVGSDTNLQLLGEAPPGVTAG
                     LPATVVRRFQEVRLTLDSRPVDMIGVDPASFASGAFWRQGFADASLGRLLGRLDQAPP
                     GRLAAVLAGPAVADARAVDVGRVRLAVTVVATARAFPGMTSSDRSLLVVSSAGFDAAA
                     GRKRYQASWWTELWARGDPGAALAALRRAGQAPAAVTTAAGQMRTAAFVAQAWTFRLL
                     SALGVLAGMVALVGLVLYAQARQRGRVIAYALTRRMGLSRAAHRRSVFWELAGLLGFA
                     FLLGAGLAVVAAAAVLPRLDPMPELPPSLRLELPGPVL"
BASE COUNT          222 a          984 c          968 g          335 t
ORIGIN      
        1 aagccctgct caaagcgccg atccggctgg tccacttccc tggggtggcg gtcgccgtgg
       61 ttgccgcggc actgatcctg gcgctgaccg cgacctcggg ccggctgttc ctggcctccg
      121 ccggcgacgc cgccgtcggc caggagctcg accggatcgg cggggtcccg atgctggcca
      181 tggtcatgtt cggggaccgg ccggcggacg tggccgcggt gcagcgggcg gccgagtcgg
      241 tcgccagcga cctggtcccc ggcctcgggc cgccggtccg caccaggtcg ggcgcgacca
      301 tggacgcggc cgccgggacc aggacggcca gggtgcgcct ggccgcccgg gacggcttcg
      361 ccgggcacat ccaggcgctg gagcgggccg gtggcgacgg cgcctggctg cccgacaccg
      421 ccgcctcctc gctcggcgtg cgggccggcc agaccgtccg cctgggactg ccgcccggcg
      481 tcagggtccg ggtcgtcggc gtctaccggg acctggccac ctccgaccag ccgctcgacc
      541 gcttctggtc tccgctgggc aacgtcatct acaccctgga ccaggaggag aggaccacgc
      601 cgcccgggct ggtgctggtc gaccccgacc ggttcctgga gctgacccgc cggctggagt
      661 ccccgggcca gctggaatgg gacttctacc cggagtcgga ccggctcacc ctgccggagg
      721 ccgaccggct ggccgcccag atccaggccg tccaggggac caccggcgac ccctgggcgg
      781 agcaggcagg gctcacccgg gtcaccaccg ccaccccgat caccgacgtg gtccgccgcg
      841 cccacgccac cctggccgcc ctgaccggac cggtggagag catctcgctc accgggcggc
      901 tcctggccct ggccctggtg gccgcggctg cggtgttcgc ggtccggcgc cgtcgggggg
      961 aggtgtcggt gctgatcgcc cacggcgttg gcgggctgcc gatcggggcg cggtcgatgg
     1021 tcgaggccgc gctccccctg gccgccgggg gcgccctcgg ctacctggtc gcgctcgggc
     1081 tggccgggcc gctggatccc gttcccggcc tccagccgca ggcgacggcg gcggccaggc
     1141 gcgaggtggc gctcgcaatg gccgccggcc tgctgctgtt cggcctggtc acctggatcg
     1201 cggtgcggtc cgaggagcgg gagcgggccc gccgcctcgg gcaggtgctg gccacgccct
     1261 ggtgggaggt gctggccctg gcgctggcgg cggcggccct gtacgagctg cggacccgcg
     1321 gcggcgcccc ggtccaggtc gagggcgagc cggcccaggt cgaccggctg ctggtgctgt
     1381 tcccgctgct ggccatcgcc ggcctggccg ggctggccac ccgcgccgcg gcccgcctgc
     1441 tgcgccgcgc ccgcccgccg ctgaccgccg cctcggcggc ccggccggcg ccggtcctgg
     1501 ccctgcgccg gctggtcgcc gctccacgga cggtcctgct gctggtcacc gggtcggcgg
     1561 tggcgctcgg gctgctgacc tactcgggga ccctggtcgc ctccacccgg tcgggggccg
     1621 tcgacaaggc cagggtcctg gtcggcagcg acaccaacct ccagctcctc ggcgaggccc
     1681 cgccgggcgt caccgccggg ctgccggcga ccgtggtgcg gcggttccag gaggtcaggc
     1741 tgacgctcga ctcccggccg gtggacatga tcggggtcga cccggcgagc ttcgcgagcg
     1801 gcgccttctg gcgccagggg ttcgccgacg cctccctcgg ccgcctgctc gggcggctgg
     1861 atcaggcccc gccgggccgg ctggccgccg tcctggccgg tccggcggtg gccgacgccc
     1921 gcgccgtgga cgtcggccgg gtccgcctgg ccgtgacggt ggtggccacg gccagggcgt
     1981 tcccgggcat gacctcgagc gaccggagcc tgctggtggt ctcctcggcc gggttcgacg
     2041 cggccgcggg acgcaagcgc taccaggcca gctggtggac cgagctgtgg gccaggggcg
     2101 acccgggagc ggccctggcc gccctgcgcc gggccgggca ggcgccggcg gccgtgacca
     2161 ccgccgccgg ccagatgcgg acggcggcgt tcgtggccca ggcctggacc ttccggctgc
     2221 tgtcggccct tggcgtcctg gccgggatgg tcgccctggt cggcctggtc ctctacgccc
     2281 aggcccgcca gcggggccgg gtcatcgcct acgccctgac caggcggatg ggcctgagcc
     2341 gggccgccca ccgccgctcg gtcttctggg agctggccgg cctgctgggc ttcgccttcc
     2401 tgctcggcgc cggcctggcc gtggtcgccg ccgcagccgt cctgccccgc ctcgacccca
     2461 tgcccgagct cccaccgagc ctccggctcg agctcccggg cccggtgct
//
LOCUS       DASSWR010000316         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_859721,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000316 DASSWR010000000
VERSION     DASSWR010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859721"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..873
                     /locus_tag="VFO47_05260"
     CDS             148..873
                     /locus_tag="VFO47_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788135.1"
                     /translation="MPRTDPQLMRQAMADFVAAVHEAYLAQARLLPPAAQARLPLLQA
                     RRLTVAAAGARNLHVIGTGERFPAPVGQEVEVAGEADGLAWDLRFFDPVVLPVLGLVA
                     EADGPDPAEVRRVLGVGTYLYHLVVEPGSQLTPHHATHAGTGLASAHAAAARELETIR
                     AHAPGRQELVDELAGATTAGLVRAQALLARELAPGDQAVAAATDPAAIRAALVKALGT
                     TERTSRSGGLPSHSGAPPPGGSG"
     gene            870..1745
                     /locus_tag="VFO47_05265"
     CDS             870..1745
                     /locus_tag="VFO47_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET8788136.1"
                     /translation="MSRARGRDGATLSSVQNAARLLKEFGTAEGSLGVTELARRLGLG
                     KSTVHRLLATLTAERILEHDPATGTYRLGLMMWELGARVSVHRVLHDAATTVIEELRN
                     ATKETVQVAVLDGREVVYVERLESPHTLRIFGRIGHRNWAHCTSTGKVLLAFQPERRR
                     QALLEGWRPERRTDATICDLAQLKAELERVRAQGWAENIGESELGVASVAAPVRNARG
                     QVIAALSVAGPVMRVNGDTLRRFFRASVIQAADAISDQLGWRPDRDRSGRGRGRAAST
                     LPTAPEHRPRVRGAS"
     gene            1742..2596
                     /locus_tag="VFO47_05270"
     CDS             1742..2596
                     /locus_tag="VFO47_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8788137.1"
                     /translation="MSSFEPGGLPVDWGSGGVEPPLPVDDLARALHRARRDRVPIPPL
                     TEERPELTAADAYAVQRRLVELLQGDGEGGIVGYKLGLTSRVMQEQLGIDQPDYGPVL
                     AGGVHPDGVGVDLDRLIQPKVEAEIAVVLDAPLAGPHCTALDVRRAAAGAVAAIEIID
                     SRVADWRIRLADTIADLASSSAIALSSHLVPLDRLDPRLVGVAMTRNGELVATGAGAA
                     ALGDPLHAVAWLANTLQPLGVTLEAGHVVMTGALHAAVPLAEGDVFRAEFDWLGPVGL
                     SVHRGGNR"
     gene            2596..>3308
                     /locus_tag="VFO47_05275"
     CDS             2596..>3308
                     /locus_tag="VFO47_05275"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HET8788138.1"
                     /translation="MLRCVVIGSGNIGTDLVHKLLRSPSLELRALVGIDPASEGLARA
                     RELGVEASADGIDWVEAHAGEVDLAFEATSARVHAANAPRLAAAGIIAIDLTPARLGP
                     AVVPGVNLDRHLHAPNLNLITCGGQATAPIVAAVASASPVPYAEIVSTVASRSAGPGT
                     RQNIDEFTRTTARSLEEVGGAAHGKAIIILNPADPPILMRNTVFCALPDGYDEEAVAA
                     AVEDMVERVAAYVPGYRLKR"
BASE COUNT          408 a         1296 c         1199 g          405 t
ORIGIN      
        1 gggcacgcgc acgtccacgt gcgacaggcg gaggatcccc atgcacgctc cttgtcgttg
       61 cggctgggca agaaggtacg gggcgtcccg ctccgcggga tagcgtccct tcccgcatgc
      121 cggaacgtgg gctatggtcg ccagacgatg ccgaggaccg acccgcagct gatgcgccag
      181 gccatggccg acttcgtggc cgccgtccac gaggcctacc tggcccaggc ccggctgctg
      241 cccccggccg cccaggcccg cctgcccctg ctccaggccc ggcggctgac cgtggccgcc
      301 gccggggccc gcaacctgca cgtgatcggg accggcgagc ggttcccggc cccggtcggc
      361 caggaggtcg aggtggccgg ggaggccgac ggactggcct gggacctgcg cttcttcgac
      421 ccggtggtcc tgcccgtgct cggcctggtc gccgaggccg acgggcccga cccggctgag
      481 gtccggcggg tgctcggggt cgggacctac ctgtaccacc tggtggtcga gcccggctcc
      541 cagctcaccc cccaccacgc cacccatgcc ggcaccggcc tggccagcgc ccacgccgcc
      601 gccgcccgcg agctggagac gatccgggcc cacgcccccg gccgccaaga gctggtcgac
      661 gagctggccg gggcgaccac cgccggcctg gtccgcgccc aggccctcct ggcccgcgag
      721 ctcgcccccg gcgaccaggc cgtcgccgcc gccaccgacc cggcggccat ccgcgccgcc
      781 ctggtcaagg ccctggggac aaccgagcgc acctcccgtt cgggtgggct accctcccat
      841 tcgggggccc caccaccggg agggtcgggg tgagccgcgc ccgggggcgg gacggggcga
      901 ccctgtcgtc ggtgcagaac gcggcccggc tgctgaagga gttcggcacg gccgaggggt
      961 cgctgggggt gaccgagctg gcccggcggc tggggctggg caagtcgacc gtgcaccggc
     1021 tgctggccac cctgaccgcc gagcggatcc tggagcacga cccggccacc gggacctacc
     1081 ggctggggct gatgatgtgg gagctggggg cgcgggtgtc ggtgcaccgg gtcctgcacg
     1141 atgccgccac caccgtgatc gaggagctgc gcaacgccac caaggagacc gtccaggtgg
     1201 ccgtcctcga cggccgtgag gtggtctacg tcgaacggct ggagagcccg cataccctgc
     1261 ggatcttcgg ccgcatcggc caccgcaact gggcccactg caccagcacc ggcaaggtgc
     1321 tgctggcctt ccagcccgag cggcgccggc aggcgctgct cgagggatgg cggcccgagc
     1381 gccgcaccga cgccaccatc tgcgacctcg cccagctcaa ggcggagctg gagcgggtcc
     1441 gggcccaggg gtgggccgag aacatcggcg agagcgagct cggggtggcc agcgtggccg
     1501 cccccgtccg caacgcccgc ggccaggtga tcgccgccct cagcgtggcc ggcccggtga
     1561 tgcgggtcaa cggcgacacc ctgcgccgct tcttccgggc ctcggtgatc caggccgccg
     1621 acgccatctc cgaccagctc ggctggcggc ccgaccgaga ccggtccggt cgaggtcgag
     1681 gccgtgccgc ctccaccctt ccgactgcgc cggagcatcg gccacgggtg cggggggcct
     1741 catgagctcc ttcgagccgg gcgggctccc ggtcgactgg ggctcgggcg gggtcgagcc
     1801 cccgctgccc gtcgacgacc tggccagggc cctgcaccgg gcccggcgcg accgcgtccc
     1861 gatcccgccc ctgaccgagg agcggccgga gctgaccgcc gccgacgcct acgccgtcca
     1921 gcgccgcctg gtcgagctcc tccagggcga cggcgagggc gggatcgtcg gctacaagct
     1981 gggcctgacc agccgggtca tgcaggagca gctcggcatc gaccagcccg actacgggcc
     2041 ggtgctggcc ggtggcgtcc accccgacgg ggtgggggtc gacctggacc ggctgatcca
     2101 gcccaaggtc gaggccgaga tcgccgtcgt gctcgacgcc cccctggccg gcccccactg
     2161 caccgccctt gacgtgcggc gggccgccgc cggagcggtg gccgcgatcg agatcatcga
     2221 ctcgcgggtc gccgactggc gcatccggct ggccgacacc atcgccgacc tcgcctccag
     2281 ctcggccatc gccctctcca gccacctggt ccccctcgac cggctcgacc cccggctggt
     2341 cggggtggcc atgacccgca acggcgagct ggtcgcgacc ggcgccggcg cggccgccct
     2401 cggcgacccg ctgcacgcgg tcgcctggct ggccaacacc ctccagccgc tcggggtgac
     2461 cctggaggcc gggcacgtgg tcatgaccgg cgccctgcac gccgccgtcc ccctggccga
     2521 gggcgacgtg ttccgggccg agttcgactg gctgggcccg gtcggcctgt ccgtccaccg
     2581 gggagggaac cgctgatgtt gcgctgcgtg gtcatcgggt cgggcaacat cggtaccgac
     2641 ctggtgcaca agctgctccg gtcgccgtcg ctggagctgc gcgccctggt cgggatcgac
     2701 ccggcctccg agggcctggc ccgggcccgg gagctggggg tggaggccag cgccgacggg
     2761 atcgactggg tcgaggccca cgccggcgag gtcgacctgg ccttcgaggc gaccagcgcc
     2821 cgggtgcatg ccgccaacgc gccccggctg gccgccgccg ggatcatcgc catcgacctg
     2881 accccggcca ggctgggccc ggcggtcgtg cccggggtca acctcgaccg gcacctgcac
     2941 gcccccaacc tcaacctgat cacctgcggc ggccaggcca cggcgccgat cgtggccgcg
     3001 gtggcctcgg cctccccggt gccgtacgcc gagatcgtct ccaccgtggc ctcccggtcg
     3061 gccgggcccg ggacccgcca gaacatcgac gagttcaccc ggaccaccgc ccgctccctt
     3121 gaggaggtcg gcggggccgc ccacggcaag gcgatcatca tcctcaaccc cgccgacccg
     3181 ccgatcctga tgcgcaacac cgtgttctgc gccctgcccg acggctacga cgaggaggcg
     3241 gtggccgccg ctgtcgagga catggtcgag cgggtggccg cctatgtgcc cggctaccgg
     3301 ctcaagcg
//
LOCUS       DASSWR010000317         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_379449,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000317 DASSWR010000000
VERSION     DASSWR010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379449"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFO47_05280"
     CDS             complement(<1..934)
                     /locus_tag="VFO47_05280"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8788139.1"
                     /translation="METFERPIRRVGVLSVHSSPLDPPGVGDSGGMNVTVRALAGELA
                     RAGIESDLYTRATSPEDPPVVEVEPGVRVLHLPAGPLEPVPKQHLPGYLCAFLCSLLR
                     AGERHGPYDVLHSHYWVSGWVARLARERWDSPVAHSFHTLGRVKNLSLADGDQPEPPT
                     RLAGEERVVAAADRLLAPTPAEARQLVELYGASPAKVSIVPHGVDRTRFHPGDRAAAR
                     AALGIAHRHVLAFVGRLQPLKAPDVAVLTLAALRRRRPDLDTELLVVGGASGNGSGEP
                     SRLARLAAAHGVADRVRFLAARPHDRLATIYRAAD"
     gene            complement(1031..1420)
                     /locus_tag="VFO47_05285"
     CDS             complement(1031..1420)
                     /locus_tag="VFO47_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788140.1"
                     /translation="MPTPTTKDAKQALYAVAGAADLAVSTIRHLPAEAAKMRSRLPGE
                     AVKTYGQLVRRGETLVTGVRRSRPTRQATEATRVAVSRTKAANTRARSSAKATRTSAK
                     SASTTVRRAAAADTKAAKAAASKVGEK"
     gene            complement(1420..1788)
                     /locus_tag="VFO47_05290"
     CDS             complement(1420..1788)
                     /locus_tag="VFO47_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8788141.1"
                     /translation="MPTGMDQLGDVGRYIRDQRRQAELSLRKLASQAGVSNPYLSQIE
                     RGLRQPSAKILKDIAKALRISAETLYVRAGILDEEAGATDLEVGILRDPHLTERQKQV
                     LIDVYRSFRAEQAADDKGVP"
     gene            complement(1859..>2129)
                     /locus_tag="VFO47_05295"
     CDS             complement(1859..>2129)
                     /locus_tag="VFO47_05295"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET8788142.1"
                     /translation="TGGRILAKVGAEAAYGAVDLATGTGLALKVLDGAPRARAAALLA
                     AVRALDWLDDDQWEAVLPAATLDIHGGGQTVGAVRPARLDLAVPD"
BASE COUNT          242 a          736 c          864 g          287 t
ORIGIN      
        1 ggtcggccgc ccggtagatg gtggccaggc ggtcgtgcgg ccgagcggcg aggaagcgga
       61 cccggtcggc gaccccgtgg gcggcggcca gccgggccag gcgggacggc tcgccgctcc
      121 cgttgcccga ggccccgccg accaccagca gctcggtgtc caggtcgggc cgccggcggc
      181 gcagggcggc cagggtgaga acggcgacgt cgggcgcctt gagcggctgg agccggccga
      241 cgaaggcgag cacgtggcgg tgggcgatcc cgagggcggc ccgggcggcg gcccggtcgc
      301 ccgggtggaa gcgggtccgg tcgacgccgt gggggacgat gctgaccttg gccggggagg
      361 cgccgtagag ctcgacgagc tggcgggcct cggccggggt cggggcgagc agccggtcgg
      421 cggccgccac cacccgctcc tcgccggcca ggcgggtcgg aggctcgggc tggtcgccgt
      481 cggccaggct gaggttcttg acccgcccga gggtgtggaa ggagtgggcc accggactgt
      541 cccagcgctc ccgggccagc cgggccaccc agcccgacac ccagtagtgg ctgtggagca
      601 cgtcgtaggg gccgtggcgc tccccggccc gcagcagcga gcagaggaag gcgcagaggt
      661 agccgggcag gtgctgcttg gggacgggct cgagcggtcc cgcgggcagg tgcagcaccc
      721 ggaccccggg ctcgacctcg accaccggcg ggtcctcggg cgaggtggcc ctggtgtaga
      781 ggtccgactc gatcccggcc ctggccagct cgccggccag ggcgcggacg gtgacgttca
      841 tgccgccgga gtcgcccacc ccgggcgggt ccaacgggga tgagtggacg gagaggaccc
      901 ccacccggcg gatcggccgc tcgaacgtct ccacccgggc tccctgggct cgctcccgct
      961 ccgcgaccgt gacgcgaagc acccgcgggt gtctacccgc gggtgctcac atgctaccgg
     1021 cgaacggtgg ttacttctcg ccgaccttgc tggccgcggc cttggctgcc ttggtgtcgg
     1081 cggcggcggc gcggcgcacg gtggtgctgg ccgacttcgc cgacgtgcgg gtggccttgg
     1141 cgctgctgcg ggcccgggtg ttggccgcct tggtgcggga cacggcgacc cgggtcgcct
     1201 cggtggcctg gcgggtcggg cgggaacggc ggaccccggt gaccagggtc tcgccccggc
     1261 gcaccagctg gccgtaggtc ttgacggcct cgccgggaag gcgggagcgc atcttggcgg
     1321 cctcggccgg caggtgccgg atggtggaga cggccaggtc ggccgcgccg gcaacggcgt
     1381 acagggcctg cttggcgtcc ttggtggtgg gcgtgggcat cagggaactc ctttgtcgtc
     1441 ggctgcttgc tcggcccgga acgagcggta gacgtcgatc aggacctgct tctgccgctc
     1501 ggtcaggtgg gggtcccgga ggatgccgac ctccaggtcg gtggccccgg cctcctcgtc
     1561 gaggatcccg gcccgcacgt acagcgtctc ggcgctgatc cgcagggcct tggcgatgtc
     1621 cttgaggatc ttcgccgacg gctgccgcag cccccgctcg atctgcgaga ggtaggggtt
     1681 cgacactccc gcctggctgg ccagcttccg gagcgacagc tccgcctggc gccgctggtc
     1741 ccgaatgtag cgacccacgt cacccagctg gtccatgccg gtcggcactc ggttcctccc
     1801 tcctgacgct agccagggta gtccggctgt ataacttttg caagcagccg cggggcggtc
     1861 agtccggcac ggcgaggtcg agcctggccg ggcggacggc ccccaccgtc tggccgccgc
     1921 cgtggatgtc gagggtggcc gcgggcagga cggcctccca ctggtcgtcg tcgagccagt
     1981 cgagggcgcg aacggcggcc aggagggcgg cggcgcgggc gcggggggcg ccgtcgagga
     2041 ccttgagggc caggccggtg ccggtggcca ggtcgacggc gccgtaggcg gcctcggcgc
     2101 cgaccttggc caggatccgg ccgccggtc
//
LOCUS       DASSWR010000318         6211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1368502, whole genome shotgun sequence.
ACCESSION   DASSWR010000318 DASSWR010000000
VERSION     DASSWR010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368502"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFO47_05300"
     CDS             <1..620
                     /locus_tag="VFO47_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8788143.1"
                     /translation="GNPKNIRTWDDLIKPGVEVIEPNPFTSGGAKWNIMAAYGAQLQA
                     GKTPEQAADYLLELFRHVPVQDKSAREALQTFIGGKGDVMLAYENEAINAQQKEQPVD
                     YVVPDSTILIENPIAVTTKAKQAATATAFVDFLRSEPAQKLYAERGYRPVNQAGAAGA
                     GFPEPSGLFTIKDLGGWPDVNKKFFDKDAGIVADIERKVGVSVGS"
     gene            670..1434
                     /gene="cysT"
                     /locus_tag="VFO47_05305"
     CDS             670..1434
                     /gene="cysT"
                     /locus_tag="VFO47_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET8788144.1"
                     /translation="MLRGTVTLYLSLIVVLPLTAVVWRSGRDGWGAFWEAATQPQALA
                     ALRLTFAVAFGVAAGNVVMGTVIAWVLVRDEFPGKRVVNALIDLPFALPTIVAGITLL
                     ALYGSASPVGVDIGYTRAGVVLALAFVTLPFVVRSVQPVLLTMEPDMEEAARCLGAGP
                     LTSFRRIVLPTIAPALLAGAALAFARAVGEFGSVVILSGNIPYQTEVASVHVFSLVEN
                     DDVGSAAAVSVVLLATSVLVLAGLNLVQRRRTRHAG"
     gene            1424..2266
                     /gene="cysW"
                     /locus_tag="VFO47_05310"
     CDS             1424..2266
                     /gene="cysW"
                     /locus_tag="VFO47_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HET8788145.1"
                     /translation="MRGSAARLPLRVLALGYLAVILLVPVAAVIWRAFGDGLAPVWQA
                     ITAPDALHALWLSLLIAAIAVPLNTVFGIACAVVLVRQRFPGRRLLDAVIDLPFAISP
                     VVVGLALILVYGQAGWLGSWLAERGIQVIFSLPGMVLATVFVSLPYVVREVAPVLREV
                     GTDQEEAAWTLGASPLRAFLQVTLPAIRWGVAYGVVLTTARALGEFGAVTIVSGRLAG
                     QTETLTLYVENRFEAFDLTGAYAASLTLAVLALLVLLAMTRLPSHRGTPSGFPGPLRR
                     KEGR"
     gene            2267..3277
                     /locus_tag="VFO47_05315"
     CDS             2267..3277
                     /locus_tag="VFO47_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HET8788146.1"
                     /translation="MSILVRNATRRFGDFTALDDVSVEIPSGSLTALLGPSGSGKSTL
                     LRVIAGLERPDAGSVEIDGRDATRLPPQRRGVGFVFQHYAAFKHMTVRENVAFGLKVA
                     RRPKAEIRARVDELLELVQLPGLADRYPAQLSGGQRQRMALARSLAVQPSVLLLDEPF
                     GALDARVRKELRAWLRHLHDEVHVTTVFVTHDQEEAMEVADRIVVMNHGRVEQVGGPR
                     DLYEHPANAFVMGFVGPVPRLGELFIRPHDVEVRRQPNGTTFEAMVERLVHLGFEVRA
                     DLLLDDGQRLQAQLSREDAEELELVEGQIVFVRPSRTRQFADARPDGRAARGLAGPRR
                     MP"
     gene            3327..5024
                     /locus_tag="VFO47_05320"
     CDS             3327..5024
                     /locus_tag="VFO47_05320"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET8788147.1"
                     /translation="MGWQWYFSAVALAGGVCLAGYTLYVWRHRRASAGASLAVMLAAA
                     GWWGLAYALELGSSRLETRQFWGDVKWLGIALLPPAFYAFVMQYTGRERWVNRRTMAA
                     LAVVPAATIVLLAVPATHDLVRYYPPEAAADPTTAIAQVGPLFWPFLVYANLVVWGST
                     ALFVWTLTRLSRLYWRQSLLLIVAVLLPSVANVAHNLNLGPFRHVEPTPFLFVLTGAV
                     LVWGVFRFQLLDLAPIARSSVFETMLDGVLVLDPYRRVVNLNPAAARALGVPAGQAVG
                     RQVEQLLGVEPSLVERADDPVLPEEVELAGRRYELSTTPLADRGGRVTGRVVVLRDVT
                     ERHQAHERLLRLDEQRRWLLGRVVWAQEEERRRIAGEVHDDAIQTIAAARLMLTTFRD
                     QLTDDRQRRLLDNLEEAVSSSLRRLRTLVFDLRPAQLDDDGLAAALREYLTETARQGG
                     FTAELRDDLEREPPAEARVIAYRICQEALTNIRMHAGATRAVVRLEESRGGLLVTIGD
                     DGAGFDPDRVKASPRPGHVGLTSMSERASLADGWCRVDSHPGSGTTVRFWLPTKDLG"
     gene            complement(5038..5811)
                     /locus_tag="VFO47_05325"
     CDS             complement(5038..5811)
                     /locus_tag="VFO47_05325"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET8788148.1"
                     /translation="MTDAVQNLVAALAAGQVEVVDLTQPLSEETPILRLPEPFANTPR
                     FRLRELSHYDERGPAWYWNAFEGGEHTGTHFDAPIHWVSGQALDDVSQVPPSRLIGPA
                     VVIDHADRAAADPDHLLRVEDVEAFEAEHGRLPDGGWLLYRTGWDARAQDEAAFLNAD
                     DTGPHTPGIDVACARWLAERPILGIGVETVGTDAGAAHSFDPPFPCHAFLLGAGKYGL
                     TQLANVDRLPPTGALVIAAPLKIVGGSGSPTRVLALVPR"
     gene            complement(5808..>6211)
                     /locus_tag="VFO47_05330"
     CDS             complement(5808..>6211)
                     /locus_tag="VFO47_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8788149.1"
                     /translation="KVVVLDGDGSVLMNLGGLTTLARYAPGNLTHIVFDNQSLLSVGG
                     FPTATATGSDLAGMAKAAGVANARTVTDLESFRAAAVEAIAAPELACLVAKVEPVGPK
                     SFHMDLHMLENRFEFQRYLRPDAPDPQGAPP"
BASE COUNT          765 a         2280 c         2259 g          907 t
ORIGIN      
        1 agggcaaccc caagaacatc cggacctggg acgacctgat caagccgggc gtcgaggtca
       61 tcgagccgaa cccgttcacc tcgggcgggg ccaagtggaa catcatggcc gcctacggcg
      121 cccagctgca ggccggcaag acccccgagc aggcggccga ctacctcctg gagctgttcc
      181 gccacgtgcc cgtgcaggac aagtcggccc gtgaggcgct ccagaccttc atcggcggca
      241 agggcgacgt gatgctggcc tacgagaacg aggccatcaa cgcccagcag aaggagcagc
      301 cggtcgacta cgtggtgccc gacagcacca tcctgatcga gaacccgatc gccgtcacga
      361 ccaaggcgaa gcaggccgcc acggccaccg ccttcgtcga cttcctccgc tcggagccgg
      421 cccagaagct gtacgccgag cgcggctacc ggccggtcaa ccaggccggg gccgccggcg
      481 ccggcttccc cgagccgtcc ggcctgttca cgatcaagga tctgggcggt tggcccgacg
      541 tcaacaagaa gttcttcgac aaggacgccg ggatcgtggc cgacatcgag cgcaaggtgg
      601 gcgtgtcggt tggcagctag caccctcgcc ggccgccggg tcgggcacga ggtccggggc
      661 ggtgccgcgc tgctgcgcgg caccgtcacc ctgtacctca gcctgatcgt cgtcctcccg
      721 ctgaccgcgg tggtgtggcg ctcgggccgc gacggctggg gcgccttctg ggaggccgcg
      781 acccagcccc aggccctggc cgcgctgcgc ctcaccttcg ccgtcgcctt cggtgtggcg
      841 gccggcaacg tggtcatggg cacggtcatc gcctgggtgc tggtccgcga cgagttcccc
      901 ggcaagcggg tggtcaacgc cctgatcgac ctgcccttcg ccctgccgac gatcgtggcc
      961 gggatcaccc tgctggccct gtacggcagc gccagcccgg tcggcgtcga catcggctac
     1021 acccgggccg gggtggtgct cgccctggcc ttcgtgaccc tgccgttcgt ggtccgctcg
     1081 gtccagccgg tgctgctgac catggagccg gacatggagg aggcggcccg ctgcctgggc
     1141 gcgggcccgc tgaccagctt ccggcggatc gtgctcccca ccatcgcccc ggccctgctg
     1201 gccggcgccg ccctcgcctt cgccagggcg gtcggcgagt tcggctcggt ggtgatcctg
     1261 tcgggcaaca tcccctacca gaccgaggtc gcctcggtgc acgtgttctc gctggtcgag
     1321 aacgacgacg tcggcagcgc ggccgcggtg tcggtggtcc tgctggccac ctcggtgctg
     1381 gtcctggccg gcctcaacct ggtccagcgg aggaggaccc gccatgcggg gtagcgcagc
     1441 ccgcctgccc ctgcgggtcc tggccctcgg ctacctggcc gtgatcctgc tggtgccggt
     1501 ggcggcggtc atctggcggg ccttcggtga cggcctggcc ccggtgtggc aggccatcac
     1561 cgccccggac gcgctccacg ccctgtggct gtcgctgctg atcgcggcca tcgccgtgcc
     1621 cctgaacacc gtgttcggga tcgcctgcgc ggtcgtgctg gtgcggcagc ggttccccgg
     1681 gcggcggctg ctggacgccg tcatcgacct gccgttcgcc atctcgccgg tggtcgtcgg
     1741 gctcgccctc atcctcgtgt acggccaggc cggctggctc ggctcctggc tggccgagcg
     1801 cggcatccag gtcatcttct cgctcccggg gatggtgctg gccaccgtgt tcgtctccct
     1861 gccgtacgtg gtccgggagg tggccccggt gctgcgcgag gtcggcaccg accaggagga
     1921 ggccgcctgg accctggggg cgtccccgct gcgggcgttc ctccaggtca ccctgcccgc
     1981 gatccgctgg ggggtggcct acggggtggt cctgaccacc gcccgggccc ttggcgagtt
     2041 cggcgccgtc acgatcgtct ccggccgcct ggccggccag accgagaccc tcaccctgta
     2101 cgtggagaac cgcttcgagg ccttcgacct caccggcgcc tacgccgcct cactcaccct
     2161 ggccgtgctc gccctgctcg tcctgctggc aatgacccgg ctcccgtccc accgaggcac
     2221 cccttcgggg ttccccggac ccctccggcg caaggaaggc cgctagatgt ccattctcgt
     2281 gcgtaacgcg acccgccgct tcggggactt caccgccctc gacgacgtgt cggtggagat
     2341 ccccagcggc tccctgaccg ccctgctcgg ccccagcggc agcggcaagt cgaccctgct
     2401 gcgggtgatc gccgggctgg agcggcccga cgccggcagc gtcgagatcg acggccgcga
     2461 cgccacccgc ctgccgccgc agcggcgcgg ggtcggcttc gtgttccagc actacgccgc
     2521 gttcaagcac atgacggtgc gcgagaacgt cgccttcggg ctcaaggtgg cccgccggcc
     2581 caaggccgag atccgggccc gggtcgacga gctgctggag ctggtgcagc tgcccgggct
     2641 ggccgaccgc tacccggccc agctctcggg cggccagcgc cagcgcatgg ccctagcccg
     2701 ctccctggcc gtgcagccga gcgtgctgct gctggacgag cccttcgggg ccctggacgc
     2761 ccgggtccgc aaggagctgc gggcctggct gcgccacctc cacgacgagg tccacgtgac
     2821 caccgtgttc gtcacccacg accaggagga ggccatggag gtggccgacc ggatcgtggt
     2881 catgaaccac ggccgggtcg agcaggtcgg cggcccccgc gacctctacg agcacccggc
     2941 caacgccttc gtgatgggct tcgtcggccc cgtcccccgc ctgggcgagc tcttcatccg
     3001 cccccacgac gtcgaggtgc gccgccagcc caacggcacc accttcgagg ccatggtcga
     3061 gcgcctggtc catctgggtt tcgaggtccg ggccgacctg cttctcgacg acggccagcg
     3121 cctccaggcc cagctgtcgc gcgaggacgc cgaggagctg gagctggtcg agggccagat
     3181 cgtgttcgtc cgacccagcc gcacccgcca gttcgccgac gccagaccgg atggccgagc
     3241 ggcccggggt ctagccggac ccaggcggat gccatagcat tccctgctta acccgaacgg
     3301 gccatcccac ccgaaaggtt cccgccgtgg gctggcagtg gtacttcagc gccgtcgcct
     3361 tggccggtgg cgtctgcctt gccggctaca ccctgtacgt gtggcggcac cggcgggcgt
     3421 cggccggggc cagcctggcc gtgatgctgg ccgcggccgg ctggtggggg ctggcctacg
     3481 ccctggagct gggctccagc cggctggaga cgaggcagtt ctggggcgac gtcaagtggc
     3541 tcggcatcgc cctgctcccc ccggccttct acgccttcgt catgcagtac acggggcggg
     3601 aacggtgggt gaaccggcgc accatggccg ccctggccgt ggtcccggcg gccacgatcg
     3661 tgctgctggc cgtcccggcc acccacgacc tggtccgcta ctacccgccg gaggcggcgg
     3721 ccgacccgac cacggccatc gcccaggtcg gccccctgtt ctggcccttc ctggtctatg
     3781 ccaacctggt cgtgtggggc tccacggccc tgttcgtgtg gaccctgacg cggctgtcgc
     3841 ggctctactg gcgccagagc ctgctgctga tcgtggccgt gctcctgccg agcgtggcca
     3901 acgtcgccca caacctcaac ctcgggccgt tccgccacgt cgagccgacc ccgttcctgt
     3961 tcgtgctcac cggggcggtg ctggtctggg gggtgttccg gttccagctg ctcgacctgg
     4021 ccccgatcgc ccgcagctcg gtgttcgaga cgatgctgga cggggtgctg gtcctcgacc
     4081 cctaccggcg ggtggtgaac ctcaacccgg cggccgcccg cgccctcggc gtgcccgccg
     4141 gccaggcggt cgggcgccag gtcgagcagc tgctgggggt cgagccgtcg ctggtcgagc
     4201 gggccgacga cccggtcctg cccgaggagg tcgagctggc cggccgccgc tacgagctgt
     4261 ccaccacccc gctggccgac cggggcggcc gggtcaccgg ccgggtggtg gtgctgcgcg
     4321 acgtgaccga gcgtcaccag gcccacgagc gcctgctccg cctggacgag cagcgccgct
     4381 ggctgctcgg gcgggtcgtg tgggcccagg aggaggagcg gcgccgcatc gccggcgagg
     4441 tccacgacga cgccatccag accatcgccg ccgcccggct gatgctgacc accttccgcg
     4501 accagctcac cgacgaccgc cagcgccggc tcctcgacaa cttggaggag gcggtgtcat
     4561 ccagcctgcg gcggctgcgg acgctggtct tcgacctgcg cccggcccag ctcgacgacg
     4621 acgggctggc cgccgccctg cgcgagtacc tgaccgagac ggcccgccag ggcggcttca
     4681 ccgccgagct ccgcgacgac ctggagcggg agccgccggc cgaggcgcgg gtgatcgcct
     4741 accggatctg ccaggaggcg ctcaccaaca tccgcatgca cgccggcgcg acccgggccg
     4801 tggtccggct ggaggagtcc cggggcggcc tgctggtgac catcggcgac gacggcgccg
     4861 gcttcgaccc ggaccgggtc aaggcctccc cccgtcccgg ccacgtcggc ctgaccagca
     4921 tgtcggagcg ggccagcctg gccgacggct ggtgccgggt cgacagccac cccggcagcg
     4981 gcaccaccgt caggttctgg ctcccgacca aggacctggg ctagcctgcc gcccacctca
     5041 cctgggaacc agggccagga cccgggtggg gctgccggag ccgccgacga tcttcagggg
     5101 ggcggcgatc acgagggcgc cggtgggcgg gaggcggtcg acgttggcca gctgggtgag
     5161 gccgtacttg ccggcgccga ggaggaaggc gtggcagggg aagggcgggt cgaagctgtg
     5221 ggcggccccg gcgtcggtgc cgacggtctc gacgccgatg ccgaggatgg gccgctcggc
     5281 cagccagcgg gcgcaggcga cgtcgatgcc gggggtgtgg gggccggtgt cgtcggcgtt
     5341 gaggaaggcg gcctcgtcct gggcccgggc gtcccagccg gtgcggtaca gcagccagcc
     5401 gccgtcgggc aggcggccat gctcggcctc gaacgcctcc acgtcctcga cccgcagcag
     5461 gtggtcgggg tcggcggcgg cccggtcggc gtggtcgatg acgaccgccg ggccgatcag
     5521 gcgcgacggc gggacctggc tgacgtcgtc cagcgcctgg ccgctgaccc agtggatggg
     5581 ggcgtcgaag tgggtgccgg tgtgctcgcc gccctcgaag gcgttccagt accaggcggg
     5641 accgcgctcg tcgtagtggg acagctcccg cagccggaac cggggcgtgt tcgcgaacgg
     5701 ctcgggcagg cgcaggatcg gggtctcctc cgacagcggc tgggtcaggt cgaccacctc
     5761 gacctgcccg gcggcgaggg cggcgaccag gttctggacg gcgtcggtca cgggggggct
     5821 ccttgggggt cgggggcgtc gggccgcagg taccgctgga actcgaagcg gttctccagc
     5881 atgtgcaggt ccatgtggaa gctcttgggg ccgaccggct cgaccttggc gaccaggcag
     5941 gccagctcgg gggcggcgat ggcctcgacg gcggcggccc ggaaggactc caggtcggtg
     6001 acggtgcggg cgttggccac cccggccgcc ttggccatcc cggccaggtc ggagccggtg
     6061 gcggtggcgg tcgggaaccc gcccacgctg agcaggctct ggttgtcgaa gacgatgtgg
     6121 gtgaggttgc cgggggcgta gcgggccagg gtggtgagcc cgcccaggtt catcagcacc
     6181 gagccgtcgc cgtcgagcac gaccaccttg c
//
LOCUS       DASSWR010000319         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_125441,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000319 DASSWR010000000
VERSION     DASSWR010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125441"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFO47_05335"
     CDS             complement(<1..340)
                     /locus_tag="VFO47_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8788150.1"
                     /translation="MVKDALTRLAGQLRGELLLPGDPGYDDARQGYNSLHDHRPAVVV
                     RPAGGDDVARALEFATGAGLEVAVRSGGHSLAGYGATARGLLVDLSAMRAVHLDPEAQ
                     VAVVDGGTTAG"
     gene            complement(389..997)
                     /gene="nadD"
                     /locus_tag="VFO47_05340"
     CDS             complement(389..997)
                     /gene="nadD"
                     /locus_tag="VFO47_05340"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007626936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET8788151.1"
                     /translation="MSRRIGVMGGTFDPIHHGHLVAAEEARWQFDLDKVVFVPTGQPW
                     QKPVGVSPAEDRYQMTVIATASNPDFAVSRLEIDHPGPTYTVDTLRRLRGQHPEDTRL
                     FFIIGADAVLQILTWKEPDQVLAQAELIAATRPGFDLDRLVSQVPGAPGRVHRMEIPA
                     LAISSSDIRARVARGAPIQYLVPDGVARYIHKHALYQPRGSA"
     gene            1211..>2163
                     /gene="ugpC"
                     /locus_tag="VFO47_05345"
     CDS             1211..>2163
                     /gene="ugpC"
                     /locus_tag="VFO47_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET8788152.1"
                     /translation="MARIELQDVSKVYTGGVRAVNALTLDIPDGDFMVFVGPSGCGKS
                     TALRMVAGLEDITEGEIRIGDRVVNDMPPKDRDIAMVFQNYALYPHMTVEDNLAFGLQ
                     LRKTPKDEQKKRVAEAAKMLALEPFLKRKPAALSGGQRQRVAMGRAIVRNPQAYLMDE
                     PLSNLDAKLRVSMRAQLSSLHERLGVTTVYVTHDQIEAMTLGSRVAVLKDGELMQVNT
                     PQMLFDAPDNLFVATFIGSPAMNLAEAKLVRDQGPMLLFAEHKVPLPEQLVSSREGLD
                     GYFDRQLIVGLRPSSMEDGAFAPQDWPRVKGEVAVTEELGS"
BASE COUNT          327 a          720 c          792 g          324 t
ORIGIN      
        1 ggccggccgt cgtcccgccg tcgacgacgg cgacctgcgc ctccgggtcg aggtggacgg
       61 cgcgcatcgc cgacaggtcg accagcagcc ctcgcgcggt ggcgccgtag ccggccaggc
      121 tgtggccgcc gctgcggacc gccacctcca gcccggcgcc cgtggcgaac tccagcgccc
      181 gggcgacgtc gtcgccgccg gccggccgga cgaccaccgc cggccggtga tcgtggaggc
      241 tgttgtagcc ctggcgggcg tcgtcgtagc cggggtcgcc ggggagcagc agctcgcccc
      301 ggagctggcc agccagcctg gtcagcgcat ccttaaccac ctgccgaccg tcacctccct
      361 gccaccgggc tcggacccgg ccagcagtct aggcactccc ccggggctgg tagagggcgt
      421 gcttgtggat gtagcgggcg accccgtcgg gcaccaggta ctggatgggg gcgccgcggg
      481 cgacccgggc gcggatgtcg ctggaggaga tagccagggc ggggatctcc atgcggtgga
      541 cccggccggg cgcgcccggc acctggctga cgaggcggtc gaggtcgaag ccgggccggg
      601 tggcggcgat gagctcggcc tgggcgagga cctggtcggg ttccttccag gtgaggatct
      661 ggaggaccgc gtcggccccg atgatgaaga acaggcgggt gtcctcggga tgctggcccc
      721 gcagccggcg cagggtgtcg acggtgtagg tcggccccgg gtggtcgatc tcgaggcggc
      781 tgacggcgaa gtccgggttg gaggcggtgg cgatcacggt catctggtag cggtcctcgg
      841 ccgggctcac cccgaccggc ttctgccagg gctggccggt gggcacgaag accaccttgt
      901 ccaggtcgaa ctgccagcgc gcctcctcgg cggcgacgag gtggccgtgg tggatggggt
      961 cgaaggtgcc gcccatgacc ccgatgcgcc ggctcatgag cgcggagtgt agcgccctga
     1021 cctcccgcgc ctgctgacct ccgctggtat cgaacatcgc gggcggctac gctactgcca
     1081 aggtcggcag agacgtcacc ggatggcgtt tctgcttgtc ttcgtcgggt aaagtctgcg
     1141 caggtcagca gggcggcccg agcgactctg ccgacaggcc ggcccgacac gggtggaggg
     1201 agatcgaggc atggcgcgca tcgaactgca ggatgtgtcc aaggtctaca ccggtggggt
     1261 ccgggcggtg aacgccctga ctctggatat ccccgacggc gacttcatgg tctttgtcgg
     1321 cccctccggc tgcggcaagt ccaccgccct caggatggtc gccgggttgg aggacatcac
     1381 cgagggcgag atccggatcg gggaccgggt ggtcaatgac atgcccccca aggaccgcga
     1441 catcgccatg gtcttccaga actacgccct gtacccccac atgacggtcg aggacaacct
     1501 cgccttcggc ctccagctca gaaagacccc caaggacgag cagaagaagc gggtcgccga
     1561 agcggccaag atgctcgccc tggaaccctt cttgaagcgc aagccggcag cgctgtcggg
     1621 tggccagcgt cagcgggtgg ccatgggccg ggcgatcgtc cgcaatcccc aggcctatct
     1681 gatggacgag ccgttgagta acttggacgc caagctccgg gtcagcatgc gggcccagct
     1741 gtccagcctg catgagcgcc tcggggtgac gaccgtctat gtgacccacg accagatcga
     1801 ggccatgacc ctgggcagcc gggtggcggt cttgaaggat ggggagttga tgcaggtcaa
     1861 caccccccag atgctgttcg acgcccccga caacctgttt gtggccacct tcatcggcag
     1921 tccggcgatg aacctggccg aggccaagct ggtccgcgac caagggccga tgctgttgtt
     1981 cgccgagcac aaggtgccgc tgcccgagca gctggtcagc tcccgggagg gcctggacgg
     2041 ctacttcgac cggcagctga tcgtggggct gcgcccctcc agcatggagg atggggcctt
     2101 cgccccccag gactggcctc gggtcaaggg cgaggtcgcg gtcaccgagg agctcggctc
     2161 cga
//
LOCUS       DASSWR010000320         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1255630, whole genome shotgun sequence.
ACCESSION   DASSWR010000320 DASSWR010000000
VERSION     DASSWR010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255630"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFO47_05350"
     CDS             <1..257
                     /locus_tag="VFO47_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788153.1"
                     /translation="VGSLDLLGEVDSGAQVDSTVTRPAASPPAKGVAPTVRLDLQTGF
                     GVIEVRRASDPRPFQEAGPFEDSFPERPERPARPTAPESP"
     gene            293..709
                     /locus_tag="VFO47_05355"
     CDS             293..709
                     /locus_tag="VFO47_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788154.1"
                     /translation="MPPLDDPPGGSTMRPQERLTGGPMRRHDLDPVSLVFGFAFTGLG
                     LLLLIGQADQALRLRWVWPLLLLALGAGILLDVTRNRSRPDPDAEATPTTPEPAFAPD
                     PDTDPALTPDPAPAPTLAPGPDADPEVAVDGREQPR"
     gene            794..1564
                     /locus_tag="VFO47_05360"
     CDS             794..1564
                     /locus_tag="VFO47_05360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8788155.1"
                     /translation="MARVDYDRMAPAYVTGRALPPEGMAPWRDAIRPWLPRDLGDRSP
                     VLDLGAGTGQFAAAIAGWFGVEVVAVEPSGGMRAQAARAFSHGGVRWVAGLAERLPLG
                     DRTCAWAWVSTVVHHLDDLEAAAAELRRVLRPGAPVLVRQAFPGRMDEISLYRRFFPA
                     AGASLVEGGGLPSVERLSAVFAGAGFRAEGLQAVDQVSATSLAAYRDKVRLRADTGLR
                     LLPDDEFAAGLAALDRAVEAETSPAPVVDRIDLLVLRR"
     gene            complement(1602..>2360)
                     /gene="hemH"
                     /locus_tag="VFO47_05365"
     CDS             complement(1602..>2360)
                     /gene="hemH"
                     /locus_tag="VFO47_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET8788156.1"
                     /translation="EQGAALQRLLDAEYGAGRWRVEVGMLHSEPRIDEAVAQLAVAGV
                     RRLVGIVLSPQWSPVIMGGYRRALDAAAWRLGPDAEVVLAGPWHRAPAFLDALAERVT
                     EALGRLDAGVPVLFTAHSLPKAVVDRDPGYLDQIAETIDAVVDRTGLDRGRWQFAYQS
                     AGHSPEEWLKPDLKDLLPGLRAAGHTAVLVVPVQFLADHLEILYDIDVAAREEAEEAG
                     LAFNRIELLNTSPAFIRALADVALDQERSAQATA"
BASE COUNT          271 a          928 c          873 g          288 t
ORIGIN      
        1 gggtcggcag cctcgacctg ctcggcgagg tcgacagcgg cgcccaggtc gactcgacgg
       61 tgaccaggcc ggcggccagc ccgccggcca agggcgtggc ccccaccgtc aggctcgacc
      121 tccagaccgg cttcggcgtg atcgaggtcc gccgggcgag cgacccccgc cccttccagg
      181 aggccggccc gttcgaggac agcttccccg agcggcccga gcggcccgcc cgccccaccg
      241 cccctgagag cccatgatgc gcccccacga cgacctcccc ggaggtgcca cgatgccccc
      301 gctggacgac ccacccggag gctccaccat gcgcccgcag gagcgcctca ccggcggccc
      361 catgcgccgc cacgacctcg accccgtgtc gctcgtcttc ggcttcgcct tcaccggcct
      421 cggcctcctg ctcctgatcg gccaggccga ccaggccctg cgcctccgct gggtctggcc
      481 cctgctcctg ctcgccctcg gcgcaggcat cctcctcgac gtcacccgca accgcagccg
      541 ccccgacccc gacgccgaag ccactcccac caccccggag cccgccttcg ctcccgaccc
      601 ggacacggac cccgccctca ctcccgaccc ggccccagct cccaccctcg ctcccgggcc
      661 cgacgcggac cccgaggtgg ctgtggacgg ccgtgagcag cctcgctgac cgtcggttac
      721 cctcccggta ggggccccga caacccgggg tggaactggt ccacgccctg agaacccggg
      781 gtggagttgc cgcatggcgc gggtcgacta cgaccggatg gcgcccgcct acgtgacggg
      841 gcgggccctg ccgccggagg ggatggcgcc gtggcgggac gcgatccggc cctggctgcc
      901 gcgggacctg ggggaccgtt ccccggtgct cgacctggga gccgggaccg ggcagttcgc
      961 ggccgccatc gccgggtggt tcggggtgga ggtcgtcgcc gttgagccgt cgggcgggat
     1021 gcgggcccag gccgcccgcg ccttctccca cgggggcgtc cggtgggtcg cggggctggc
     1081 cgagcggctg ccgctggggg accggacctg cgcctgggcc tgggtgtcga ccgtcgtcca
     1141 ccacctcgac gacctggagg cggccgcggc cgagctgcgc cgggtgctcc ggccgggcgc
     1201 gcccgtgctg gtgcgccagg cctttcccgg ccgcatggac gagatctccc tgtaccgccg
     1261 gttcttcccg gcggccgggg ccagcctggt cgagggcggc gggctgccga gcgtggagcg
     1321 gctgtcggcc gtcttcgccg gcgccgggtt ccgggccgag ggcctccagg cggtggacca
     1381 ggtcagcgcg accagcctgg ccgcctaccg ggacaaggtc cgcctccggg ccgacaccgg
     1441 cctgcgcctg ctgcccgacg acgagttcgc cgccgggctg gccgccctcg accgggccgt
     1501 ggaggcggag acgtccccgg ccccggtcgt cgaccggatc gacctgctgg tgctgcgccg
     1561 ctgatccacc gggggccagg cttgagcccc ccggaccccg gctaggcggt ggcctgggcg
     1621 gagcgctcct ggtcgagggc gacgtcggcc agggcgcgga tgaaggccgg ggaggtgttc
     1681 agcagctcga tccggttgaa ggcgagaccg gcctcctcgg cctcctcccg ggcggcgacg
     1741 tcgatgtcgt agaggatctc caggtggtcg gcgaggaact ggacgggcac gaccagcacc
     1801 gcggtgtgcc cggcggcccg gaggccggga aggaggtcct tgaggtcggg cttgagccac
     1861 tcctcggggc tgtggccggc cgactggtag gcgaactgcc agcgcccccg gtccaggccg
     1921 gtccggtcga ccacggcgtc gatcgtctcg gcgatctggt ccagatagcc ggggtcgcgg
     1981 tcgaccaccg ccttgggcag gctgtgggcg gtgaacagca ccggcacgcc ggcgtccagc
     2041 cggcccaggg cctcggtgac ccgctcggcc agggcgtcca ggaaggccgg ggcgcggtgc
     2101 caagggccgg ccaggacgac ctcggcgtcg gggccgagcc gccaggcggc tgcgtccagg
     2161 gccctgcggt agccgcccat gatcaccggc gaccactggg gcgagaggac gatcccgacc
     2221 agccgccgca ccccggcgac ggccagctgg gccaccgcct cgtcgatccg gggctcggag
     2281 tggagcatgc cgacctcgac ccgccagcgg ccggcgccgt actcggcgtc gagcagccgc
     2341 tgcagggcgg ccccctgctc
//
LOCUS       DASSWR010000321         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1341022, whole genome shotgun sequence.
ACCESSION   DASSWR010000321 DASSWR010000000
VERSION     DASSWR010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341022"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="VFO47_05370"
     CDS             complement(<1..144)
                     /locus_tag="VFO47_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET8788157.1"
                     /translation="MAFTPESDDALVVVDVQRDFCPGGSLAVPDGDQVVPVINRLAPK
                     FQTV"
     gene            447..1292
                     /locus_tag="VFO47_05375"
     CDS             447..1292
                     /locus_tag="VFO47_05375"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET8788158.1"
                     /translation="MANRDELLVSTDWLAANLDDPGIRVVEVDEDTDAYARGHIRGAV
                     AVNWNTDLQDPLRRDFIAPEQFAKLQDTLGITPETTVILYGGNNNWFAAYAYWYYKVY
                     GHGSVKLLDGGRKAWELEGRELTGDPADVQSTSGYPVREPDTSIRAFRDQVLETVRTG
                     EGASLVDVRSPEEFRGEKLAPDHLPQEAAQRPGHIPGAVNIPWAKSVDGSTGKFLTDD
                     QLAELYGAQGVEAGKDVVAYCRIGERSAHTWFVLHELLGYPRVRNYDGSWTEWGSLVG
                     VPIER"
     gene            1338..3422
                     /locus_tag="VFO47_05380"
     CDS             1338..3422
                     /locus_tag="VFO47_05380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET8788159.1"
                     /translation="MDRPSDLPPPGPDGASGAILGDVLGQLAEGVAVVDASGRLRYAN
                     AEALRIMGLTGLGQLPGSFPELAARFDVRDPDGEPVPVEQWPAARVLAGERVEELQFQ
                     FVGQDGVARWVRCTGGPVELDGGEAGAWVSFRDVGREETILRELREERDLVTALVDMS
                     PLAVAVARAPDMVVELANDVAKALRPDKPMVGAPVAEVFPEAGPAGFLHLLLQVAETG
                     QTVAVEDAALEWWEPGRPRSFSMTCSRLPQPSGQPTRVLIIARETTAEVTARARAEAI
                     AAERSRDLTRARVTSARLRSLVDLAAAIASVEDLNALLYLVTGEAARLLGSDSASLFL
                     LDERGDVLAGRAHVGLDDEVVLGMRIVLAEWPEVAEVVRTGRPVVHARADQVRGPERP
                     YIERFGVRSYVAVRLGSPGRPLGVLFVNYTQRRHRFSRPELAFLETLTSYLSVIIERA
                     RLVSVLREAVTSLQAAVLPADFPTVPGLDAGALYRSASEVAQVGGDFYDLFPLEGGRA
                     AAVIGDVCGKGVAAARHTVRLRYELRTLVEEGRTPGRVLTAFNRRVLEEFVEDEYATL
                     LLLVLDPASGEVSWSSAGHPPPLLTGPTPRMLAFAGSLPVGLFPEATYQTARFTLPPG
                     RCVVLYTDGVVEARNPERQEFGTEGLEAAPPAVAASAAAVAEVVLKQVLSHSGGHLDD
                     DAAVLVLRREPA"
     gene            complement(3447..>3821)
                     /locus_tag="VFO47_05385"
     CDS             complement(3447..>3821)
                     /locus_tag="VFO47_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET8788160.1"
                     /translation="ADLARYRNNWRPQLVPLWNLVVNLRDGDRVLATLAGAAGNVVLF
                     LPLGFLLPMLAPRLGRFWPAVGAGFALSAAIELSQVAFPGVRRADVNDVLMNTLGAAL
                     GHLLYRRAERATAWSRPSADDQ"
BASE COUNT          488 a         1428 c         1398 g          507 t
ORIGIN      
        1 cacggtctgg aacttcggtg cgagccggtt gatgacgggg acgacctggt cgccgtcggg
       61 gacggccagg ctccctcccg ggcagaagtc gcgctgcacg tcgaccacga ccagcgcgtc
      121 gtcgctctct ggcgtgaagg ccatcggggc tccttgggac tgctcgaacg gacggttcgt
      181 tgacggctgc ccgctctgct ggcagcctgt accaccatga tcgtactgat ggcccagccg
      241 gaagtggatc ccgtgaccgt ccgccggggt cccgccgcgc tgaccacccg tgcgtgcgct
      301 cccatggggc tgggcacggg gaactcggcc gccggacgcc cgccgtgtcg gcgggtcgcg
      361 gcgcagctcg ccccgcgcta ggggctggag ccgggccgcg gccgcttcct catctcccca
      421 cgctgcccga ggaggcatcc gtacacatgg ccaaccgtga cgagctgctg gtctccaccg
      481 actggctggc tgccaacctg gacgaccccg gcatccgcgt cgtcgaggtc gacgaggaca
      541 ccgacgccta cgcccgcggg cacatccgcg gggccgtggc cgtcaactgg aacaccgacc
      601 tgcaggaccc gctgcgccgg gacttcatcg cccccgagca gttcgccaag ctgcaggaca
      661 ccctgggcat caccccggaa acgaccgtga tcctgtacgg gggcaacaac aactggttcg
      721 ccgcctacgc ctactggtac tacaaggtct acggccacgg atcggtcaag ctcctggacg
      781 gcggccgcaa ggcgtgggag ctggagggcc gcgagctcac cggcgacccg gccgacgtgc
      841 agtcgaccag cggctacccg gtgcgcgagc ccgacacctc catccgtgcc ttccgcgacc
      901 aggtcctgga gaccgtccgg accggcgagg gcgcgtccct ggtcgacgtc cgctcgcccg
      961 aggagttccg cggcgagaag ctcgcccccg accacctgcc ccaggaggcc gcccagcggc
     1021 cgggccacat cccgggcgcg gtcaacatcc cctgggccaa gtcggtcgac ggctccaccg
     1081 gcaagttcct caccgacgac cagctggccg agctgtacgg cgcccagggt gtcgaggccg
     1141 gcaaggacgt ggtcgcctac tgccgcatcg gcgagcgctc ggcccacacc tggttcgtcc
     1201 tccacgagct gctcggctac ccgcgggtcc gcaactacga cggctcctgg accgagtggg
     1261 gctccctggt cggggtgccg atcgagcggt aactccctgc attgctgcta gggcgggcgg
     1321 tcgggcagac tgcggccgtg gacaggccaa gcgatctgcc tccgcccggc cccgacggag
     1381 cgtcgggcgc gatcctgggc gacgtcctcg gccagctggc cgagggggtg gcggtcgtgg
     1441 acgcctccgg ccgcctccgc tacgccaacg ccgaggccct ccgcatcatg ggcctcaccg
     1501 gcctgggcca gctgcccggt tccttcccgg agctggcggc ccgcttcgac gtgcgcgacc
     1561 ccgacgggga gcccgtcccg gtcgagcagt ggccggccgc ccgggtcctg gccggcgagc
     1621 gcgtcgagga gctccagttc cagttcgtgg gccaggacgg ggtcgcccgc tgggtccgct
     1681 gcaccggcgg cccggtcgag ctggacggcg gcgaggccgg cgcctgggtc agcttccgcg
     1741 acgtcgggcg cgaggagacg atcctgcgcg agctgcggga ggagcgcgac ctggtcaccg
     1801 ccctggtcga catgagcccg ctggccgtgg ccgtggcccg cgcccccgac atggtcgtcg
     1861 agctggccaa cgacgtggcc aaggccctgc gcccggacaa gccgatggtc ggggcgccgg
     1921 tggccgaggt gttccccgag gccgggccgg ccggcttcct ccacctgctg ctgcaggtgg
     1981 ccgagaccgg ccagacggtg gcggtcgagg acgccgccct ggagtggtgg gagccgggcc
     2041 ggccccgctc gttctccatg acctgctcgc gcctgcccca gccgagcggc cagccgaccc
     2101 gggtgctgat catcgccagg gagaccacgg ccgaggtgac ggcccgggcc cgggccgagg
     2161 cgatcgcggc cgagcgcagc cgggacctga cgcgggcccg ggtcacctcg gcgcggctgc
     2221 gcagcctggt cgacctggcc gccgccatcg cctcggtcga ggacctgaac gccctgctgt
     2281 acctggtcac cggggaggcg gcgcggctgc tgggctccga ctcggccagc ctgttcctgc
     2341 tcgacgagcg gggcgacgtg ctcgccgggc gggcccacgt cggcctggac gacgaggtcg
     2401 tgctcggcat gcgcatcgtc ctcgccgagt ggccggaggt ggccgaggtc gtgcgcaccg
     2461 gccggccggt cgtgcacgcc cgggccgacc aggtccgtgg gcccgagcgg ccctacatcg
     2521 agcgcttcgg ggtccgctcc tacgtggccg tgcgcctggg cagtccgggc cggccgctgg
     2581 gggtcctgtt cgtcaactac acccagcggc ggcaccgctt cagccggccc gagctggcct
     2641 tccttgagac cctgaccagc tacctgagcg tcatcatcga gcgggcccgg ctggtcagcg
     2701 tgctccgcga ggcggtcacc tccctccagg cggccgtgct gccggccgac ttcccgaccg
     2761 tgcccggcct ggacgccggc gccctgtacc gatcggccag cgaggtggcg caggtcggag
     2821 gcgacttcta cgacctgttc ccgctggagg gagggcgggc cgcggccgtc atcggcgacg
     2881 tctgcggcaa gggcgtggcc gcggccaggc acaccgtccg gctccgctac gagctgcgca
     2941 ccctggtcga ggagggccgc acgccgggcc gggtcctgac cgccttcaac cggcgggtcc
     3001 tggaggagtt cgtggaggac gagtacgcga ccctgctgct gctcgtcctt gaccccgcgt
     3061 ccggcgaggt gagctggtcc agcgccggcc acccgccccc gctgctcacc gggcccaccc
     3121 cgcggatgct ggccttcgcc gggtcgctgc cggtcgggct gttccccgag gccacctacc
     3181 agacggcccg gttcaccctg ccgccgggcc gctgtgtggt gctctacacc gacggcgtgg
     3241 tcgaggcccg caaccccgag cggcaggagt tcgggaccga agggctggag gcggcccctc
     3301 cggcggtcgc ggccagcgcc gccgccgtgg ccgaggtcgt cctcaagcag gtcctcagcc
     3361 actccggcgg ccacctcgac gacgacgccg ccgtcctagt cctgcgccgc gagccggcct
     3421 agtcgaccgg ctccgccggt cgaagtctat tggtcgtcgg ccgaaggccg actccaagcc
     3481 gttgcgcgct cggcccggcg gtagaggagg tgcccgaggg cggcgccgag ggtgttcatc
     3541 agcacgtcgt tgacgtcggc ccggcggacc ccggggaagg cgacctggga cagctcgatg
     3601 gccgccgaca gcgcgaaccc ggcgcccacg gccggccaga accggccgag ccggggcgcc
     3661 agcatcggca gcaggaaccc caggggcagg aacaggacga cgttgccggc ggcgccggcc
     3721 agggtggcca ggacccggtc gccgtcgcgc aggttgacga ccagattcca gagcggcacc
     3781 agctggggcc gccagttgtt gcggtagcgg gccaggtcgg c
//
LOCUS       DASSWR010000322         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_577836,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000322 DASSWR010000000
VERSION     DASSWR010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577836"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..414
                     /locus_tag="VFO47_05390"
     CDS             106..414
                     /locus_tag="VFO47_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788161.1"
                     /translation="MPAPAPAIVYAEPLPAEVIDEAGAVVSVDGRGLLGSPPSRVRVQ
                     GRFWAAITAWAGPWLVDERWWDPAAHRRLVRLQATTDQGAAYLLRLAGGRWWVEAVYD
                     "
     gene            510..809
                     /locus_tag="VFO47_05395"
     CDS             510..809
                     /locus_tag="VFO47_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET8788162.1"
                     /translation="MATTGIRVTPEQLQQISAQLDAGASSIEGTLRQLAANVAPLGSD
                     WAGVAQARFLELWAEWQRSGTSLEQALTGIARLTARAGASYESAEHGIAASFGRP"
     gene            1073..>2073
                     /locus_tag="VFO47_05400"
     CDS             1073..>2073
                     /locus_tag="VFO47_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET8788163.1"
                     /translation="MGWQNPPLPWSELERRLAGRPPPPNQPPEADGGDAPAWSSRRQP
                     YQSPAEAPAPRGRRSRVVVPYAELHCHSNASFLDGGSHPEELAEEAARLRLEALAVTD
                     HDGMYGIVRFAEAARALALPTVFGAELTLTPFGARGAAGKAGKRTGTPDPAGEHLVVL
                     ARGPEGYARLCRAISEAQMAGAKGAPRGDLGRLAGLHGGHWAVLTGCRKGAVPRALAE
                     RGPAAAAVQLDRLVQAFGRDNVFVEVWDHGDPLDSARNDALVRLADRARVEVVATITV
                     HYATPARRRLATALAAVRARSSLAELDGWLPAGAGAHLRSGLEQATRLARYPGTVERA
                     "
BASE COUNT          266 a          752 c          783 g          272 t
ORIGIN      
        1 ccggatcccg ccctcccggg ccgcgtccgg ccggcccctg gcccggccag gggatcgagg
       61 tctggccctg aggggcttcc gggggctgtg ccctggcctg ggcaggtgcc ggcgccggcg
      121 ccggcgatcg tgtacgccga gccgctcccc gcggaggtca tcgacgaggc gggcgcggtc
      181 gtctcggtcg acggtcgcgg cctgctcggc tccccgccga gccgggtccg ggtccagggg
      241 cggttctggg cggccatcac cgcctgggcc ggcccctggc tggtcgacga gcgctggtgg
      301 gacccggccg cccaccgccg cctggtccgc ctccaggcca ccaccgacca gggcgccgcc
      361 tacctgctcc ggctggccgg cggccgctgg tgggtcgagg ccgtctacga ctgagtgatc
      421 ccaggagggg ctgtggacat tcgtttgcgc ccgtttccct ccgaacgtag aactgctccg
      481 accctgcggc accgcatgga aggagcgaca tggccaccac cggcatccgg gtgacccccg
      541 aacaactcca gcagatctcg gcccagctcg acgccggcgc gtccagcatc gagggcaccc
      601 tgcggcagct cgccgccaac gtggccccgc tcgggtccga ctgggccggc gtcgcccagg
      661 cccggttcct cgagctctgg gccgaatggc agcgcagcgg cacctccctg gagcaggccc
      721 tgaccggcat cgcccgcctc accgcccgcg ccggcgccag ctacgagtcg gccgagcacg
      781 gcatcgccgc cagcttcggc cgcccctgac cgttcggcga gaacggtcga gaggttatcg
      841 gctcgagaga tggctcggct ggccgggcac cgtggggcca gtcggccgtg cctcgaacgg
      901 ccgcccggag caggcggttc ggctcgggac actagcgaat ctggaggtat ctgtacgaga
      961 aattatcaat tgaacattgc ggagagaatt ctgcgaaatc gggccctgcg cggcgctcgg
     1021 gaattcagcg ccaaacttgt cagagcagaa catatgttcg atcatccggg gcatgggatg
     1081 gcaaaatcct ccgcttcctt ggtccgagct ggaacgccgc ctcgccggcc ggccacctcc
     1141 ccccaaccag cctcccgaag cggatggagg agatgcccca gcctggtcat ccaggcgcca
     1201 gccctaccag tcgcccgccg aggctccagc cccgcggggg cggcggagca gggtggtggt
     1261 tccgtatgcg gagctgcact gccattcgaa cgcgagcttt ctggatgggg ggtcgcatcc
     1321 ggaggagctg gcggaggagg cggcgcggct ccggttggag gcgctggccg tcaccgacca
     1381 cgatgggatg tacggcatcg tccggttcgc cgaggcggcc agggcgctcg cccttcccac
     1441 cgtcttcggg gccgagctca ccctgacgcc cttcggcgca cggggagccg cggggaaggc
     1501 ggggaagcgg accgggaccc ccgacccggc cggggagcac ctggtcgtgc tggcgcgggg
     1561 gccggagggg tacgcgcggc tgtgccgggc catctcggag gcgcagatgg cgggagccaa
     1621 gggggcgccc cggggggacc tggggcggct ggcggggctg cacggcgggc actgggcggt
     1681 gctgaccggg tgccgcaagg gggcggtgcc acgggccctt gccgagaggg ggccggcggc
     1741 ggccgcggtc cagctcgacc ggctggtcca ggcgttcggc cgggacaacg tcttcgtgga
     1801 ggtctgggac cacggcgacc cgctcgactc ggcccgcaac gacgccctgg tccgcctggc
     1861 cgaccgggcc agggtcgagg tggtggcgac catcaccgtc cactacgcga ccccggcccg
     1921 ccgccggctg gccaccgccc tggccgcggt ccgggcccgc agctcgctgg ccgagctgga
     1981 cggctggctg cccgccgggg ccggcgccca tctgcggtcc gggctcgagc aggcgacgcg
     2041 cctggcccgc tacccgggaa cggtcgagcg ggc
//
LOCUS       DASSWR010000323         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_95317,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000323 DASSWR010000000
VERSION     DASSWR010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95317"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1115
                     /locus_tag="VFO47_05405"
     CDS             150..1115
                     /locus_tag="VFO47_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET8788164.1"
                     /translation="MTALGAASLRWFGTTALPSPPKKENGDRLLDYAVDPAKALLILA
                     AAWVANRLVRRAIRRLVASMSEDRGLAALRAPTALARTGEIPSLRRVQRAETVGALLK
                     SVASFGIWTLAALMALGTLGLNLGPLIAGAGIVGVAVGFGSQNLVRDFISGIFMLMED
                     QYGVGDVVDAGPATGTVEGVGLRTTRLRDVNGTLWHIPNGEIRRVGNRSQGWARALVD
                     VEVAYSTDLDDATRTIERVAHELSVDEHWAAKLLEQPEVWGVEELGPDGIRVRLVAKT
                     RPLEQWKVARELRARLKVAFDKAGIEVSAQKALTSDGDEQGQPAA"
     gene            1153..2391
                     /gene="glgC"
                     /locus_tag="VFO47_05410"
     CDS             1153..2391
                     /gene="glgC"
                     /locus_tag="VFO47_05410"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET8788165.1"
                     /translation="MANPDVFTIVLAGGEGKRLAPLTLDRAKPAVAFGGDYRLVDFVL
                     SNLVNGGYMRIAVLTQYKSHSLDRHVTTSWRLSPLLGNYVTPVPAQMRRGPRWFAGSA
                     DAIFQSLNLVYDERPEHVIVFGADHVYRMDPRQMVEQHVASGAGVTVAGIRVPIGEAN
                     QFGVIETAADGRRISAFREKPSDPQGLPDAPDQVFASMGNYVFRADTLIKAVSLDAKD
                     EDSEHDLGGNIIPMMVERGEAEVYDFADNEVPGATDRDRGYWRDVGTLDAYYEAHMDL
                     ISVHPVFNLYNRKWPILSWHEPLPPAKFVFEDGEGRTGQALDSMVSAGVVVSGGTVRR
                     SILSPGVRVHTGARVEGSVVMHDVDVGRGAVVRNAIVDKNVQIPEGARIGVDLDRDRE
                     RFVVSDGGVVVIGKGQKIDA"
     gene            complement(2360..>3385)
                     /locus_tag="VFO47_05415"
     CDS             complement(2360..>3385)
                     /locus_tag="VFO47_05415"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8788166.1"
                     /translation="ATLIVPYLPRRLRWVAVVVAAVVCVSRLYVGVHLPLDVVGGAAL
                     GWAAGSLVHLLLGAPGGRPSPERAAKALREQGLQVTGVAPLGGRDARRSARFEAVGPD
                     GQHLFAKLIPRERRDEDLLYRLWRMLVRRRAEPGRRGTGPPLQQVEHEAYLGLLAGTA
                     GVRTPRVVLAGPYGSGAGLLVQRMIPGRSLEEADPAAVGDATLEDAWAEVARLHQAGI
                     AHGDLGRHSVEVDEDGRPWLVDFDHATAVASERLRQADLVELLVSLAARFGPGRAAAT
                     AVAALGSAPLAAALAAVTPAALTRTTREELSDHPGLWDDLARRVAPEAAEEGDAAVRR
                     RSSDPSR"
BASE COUNT          462 a         1232 c         1275 g          416 t
ORIGIN      
        1 cctcctggag ggcggcgccg gggtcgggca cgcggaaggt cacccgggcc gccccgacca
       61 ccgggccggc ggcggcgccg gccgtcccgc tggccggatc catcgccgga gcatatcgcc
      121 gggccgccga cagccctcga cctgggcggt tgacagccct cggagcagct tcgctacggt
      181 ggttcggaac gaccgctctc ccgtcccccc caaaaaaaga aaatggtgat cgtctgctcg
      241 actacgctgt cgaccccgcc aaggcgctgc tgatcctggc cgccgcctgg gtggccaacc
      301 ggctggtgcg ccgggccatc cggcgcctgg tcgcctccat gagcgaggac cgcgggctgg
      361 ccgccctgcg cgcccccacc gccctggccc ggaccgggga gatcccgagc ctgcgccggg
      421 tgcagcgggc cgagaccgtc ggcgccctgc tcaagagcgt ggcctccttc gggatctgga
      481 ccctggccgc gctgatggcc ctcggcaccc tggggctcaa cctgggcccg ctgatcgccg
      541 gggccgggat cgtcggcgtg gccgtcggct tcggctccca gaacctggtc cgggacttca
      601 tctccggcat cttcatgctc atggaggacc agtacggggt cggcgacgtg gtcgacgccg
      661 gcccggccac cggcacggtc gagggcgtcg gcctgcgcac cacccggctg cgggacgtca
      721 acggcaccct ctggcacatc cccaacggcg agatccgccg ggtcggcaac cgctcccagg
      781 gctgggcccg ggccctggtc gacgtcgagg tcgcctactc gaccgacctc gacgacgcca
      841 cccggacgat cgagcgggtc gcccacgaac tgtccgtgga cgagcactgg gcggccaagc
      901 tcctcgagca gcccgaggtc tggggggtcg aggagctcgg tcccgacggc atccgcgtcc
      961 gcctggtcgc caagacccgt cccctggagc agtggaaggt cgctcgcgag ctccgggccc
     1021 ggctcaaggt cgccttcgac aaggccggca tcgaggtctc ggcccagaag gccctcacca
     1081 gcgacggcga cgaacagggc cagccggccg cctgagaaga ttcgcgcgtc ccggtacggg
     1141 taggctttcg gcatggcgaa ccccgatgtg ttcacgatcg tcctcgccgg gggtgagggg
     1201 aagcggctgg cgccgctgac gctggaccgg gccaagccgg cggtggcgtt cggaggggac
     1261 taccggctgg tcgacttcgt gctgtcgaac ctggtcaacg gcggctacat gcggatcgcg
     1321 gtgctgaccc agtacaagag ccacagcctg gaccggcacg tcaccaccag ctggcggctg
     1381 tcgccgctgc tgggcaacta cgtgaccccg gtgccggcgc agatgcggcg ggggccgcgc
     1441 tggttcgcgg gatcggcgga cgcgatcttc cagagcctca acctggtcta cgacgagcgc
     1501 cccgagcacg tgatcgtgtt cggggccgac cacgtctacc ggatggaccc ccgccagatg
     1561 gtggagcagc acgtcgcgtc gggcgccggg gtgacggtgg ccgggatccg ggtgccgatc
     1621 ggcgaggcca accagttcgg ggtcatcgag accgccgccg acgggcgccg catcagcgcc
     1681 ttccgggaga agccgagcga cccccagggc ctgcccgacg cgcccgacca ggtgttcgcg
     1741 tccatgggca actacgtgtt ccgggccgac accctgatca aggccgtgtc cctggacgcc
     1801 aaggacgagg actccgagca cgacctgggc ggcaacatca tccccatgat ggtcgagcgg
     1861 ggcgaggccg aggtctacga cttcgccgac aacgaggtgc ccggggccac cgaccgggac
     1921 cgcggctact ggcgggacgt cggcaccctg gacgcctact acgaggccca catggacctg
     1981 atctcggtcc acccggtgtt caacctctac aaccgcaagt ggccgatcct gagctggcac
     2041 gagcccctgc ccccggccaa gttcgtgttc gaggacggcg aggggcggac cggccaggcg
     2101 ctcgactcga tggtctcggc cggggtggtg gtgtcgggcg gcacggtgcg gcgctcgatc
     2161 ctgtcgccgg gggtgcgggt ccacaccggc gcccgggtcg aggggtcggt ggtgatgcac
     2221 gacgtcgacg tgggccgggg cgcggtcgtc cgcaacgcca tcgtcgacaa gaacgtgcag
     2281 atccccgagg gcgcccggat cggcgtcgac ctggaccggg accgcgagcg gttcgtggtc
     2341 tccgacggcg gcgtggtcgt catcgggaag ggtcagaaga tcgacgcctg acggcggcgt
     2401 ctccctcctc ggccgcttcg ggggcgaccc ggcgggccag gtcgtcccag aggccggggt
     2461 ggtcggacag ctcctcccgg gtcgtgcggg tgagggcggc cggggtgacg gcggccaggg
     2521 cggcggccag cggcgcggag ccgagggcgg ccacggcggt ggcggcggcc cgtccgggcc
     2581 cgaagcgcgc ggccaggctg acgagcagct cgaccaggtc ggcctggcgc agccgttccg
     2641 aggcgacggc ggtggcgtgg tcgaagtcga ccagccaggg ccggccgtcc tcgtcgacct
     2701 ccacgctgtg gcggcccagg tcgccgtggg cgatgccggc ctggtggagg cgggccacct
     2761 cggcccaggc gtcctcgagg gtggcgtcgc cgacggccgc cgggtcggcc tcctccaggg
     2821 agcggccggg gatcatccgc tgcaccagca gcccggcccc gctgccgtac ggcccggcca
     2881 ggaccacccg gggggtgcgg accccggccg tgccggcgag cagccccagg taggcctcgt
     2941 gctcgacctg ctggaggggc gggccggtgc cccggcggcc cggctcggcc cggcgccgga
     3001 ccagcatccg ccagagccgg tagaggaggt cctcgtcgcg ccgctcgcgg gggatgagct
     3061 tggcgaacag gtgctggcca tcggggccga cggcctcgaa gcgggccgag cggcgggcgt
     3121 cgcgcccacc cagcggggcc accccggtca cctggagccc ctgctcgcgc agggccttgg
     3181 ccgcccgctc gggcgagggc cggcccccgg gggcgcccag cagaaggtgg accagcgacc
     3241 cggccgccca ccccagggcg gccccgccga ccacgtccag gggcaggtgc accccgacgt
     3301 acagccgcga cacgcagacc accgcggcca ccaccaccgc cacccagcgg agccgccgcg
     3361 gcaggtaggg gacgatcagg gtggc
//
LOCUS       DASSWR010000324         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1368795, whole genome shotgun sequence.
ACCESSION   DASSWR010000324 DASSWR010000000
VERSION     DASSWR010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368795"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFO47_05420"
     CDS             <1..858
                     /locus_tag="VFO47_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET8788167.1"
                     /translation="CECRPLTATPATLMAEPRDYTTVGHLYRSIGHGLARLVERYGEA
                     EVFIGPPEAQATRERLGWPELVAITDLASANAAIATIVEQGEGADGDWREAHFGRLST
                     ILEEYLAATEADPSFEPARPAQPAYVRRPPDQPLAVVISDPLTAQVADVFDAAHQTLF
                     QLLGRLFVHGGETDREVDTLAGAAIGLMATVLRPLGSVLTTLPLGPDHPGGLAGPAFF
                     MVHPSQFLLPWREAAWKVMIQRLGVMADACVRLGCEPGLERLAELEEPVRGLAARLQG
                     HLDERAAAG"
     gene            1040..2119
                     /locus_tag="VFO47_05425"
     CDS             1040..2119
                     /locus_tag="VFO47_05425"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8788168.1"
                     /translation="MLRARWLQSLERAGVPLEGLAAAVRDGALSLSFLDVAAYDRFAG
                     LSGTTFHQLSASTGIPLELLMVLREAVGFAEPRPEDHVHHNELSVVPLIQLQLAKGFQ
                     PVVIEGWLRVYGDSLRRIAEAEAAWWNSEVEMALLASGMTEGEMLRAQADLGSQMTPL
                     IEQALLAVYHGQQEHAWSQVFVEHVERALEAAGLYRRLDRPPAMCFLDITGYTRLTEE
                     RGDQAAADLAARLAGLVRRSAQEHGGTPVKWLGDGVMFYFRAPPAGVLAALEMVAGVG
                     RQGLPPAHVGIHAGPVIFQDGDYFGRTVNLAARIAEYARPNEVLVSQEVIDAADGAPV
                     TFTDIGPVELKGVPGPLRLYTARHA"
BASE COUNT          302 a          786 c          805 g          307 t
ORIGIN      
        1 tgcgagtgcc ggcccctgac cgcgaccccg gccaccctga tggccgaacc gcgggactac
       61 acgaccgtcg ggcacctgta ccgcagcatc ggccacggcc tcgcccgcct ggtcgagcgc
      121 tacggcgagg ccgaggtgtt catcggtccg cccgaggccc aggccacccg cgagcggctc
      181 ggctggcccg agctggtcgc gatcaccgac ctggcctcgg ccaacgccgc catcgcgacc
      241 atcgtcgagc agggcgaggg cgccgacggc gactggcgcg aggcccactt cggccgcctc
      301 tccaccatcc tggaggagta cctggccgcg accgaggcgg acccgagctt cgagccggcg
      361 cggccggccc agcccgccta cgtgcgccgc ccgcccgacc agccgctggc cgtggtcatc
      421 agcgacccgc tgaccgcgca ggtggccgac gtgttcgacg ccgcccacca gacgctgttc
      481 cagctgctgg gccggctgtt cgtccacggg ggcgagaccg accgggaggt ggacaccctg
      541 gccggcgccg ccatcgggct gatggcgacg gtgctgcggc cgctggggtc ggtgctgacg
      601 acactgccgc tgggtcccga ccacccgggg gggctggccg gcccggcctt cttcatggtc
      661 cacccgtccc agttcctact gccctggcgg gaggccgcct ggaaggtgat gatccagcgc
      721 ctgggcgtga tggccgacgc ctgcgtgcgg ctcggctgcg agcccggcct ggaacggctg
      781 gccgagctcg aggagccggt ccgcggcctg gccgcccggc tgcagggcca cctggacgaa
      841 cgggccgccg ccggataggc agcggatcga acgtccgctc cactcgggtc aggtccgcag
      901 ctatgctggc ggaggcgtga gctgatacac cacacaggag gtcgcccagc gggccggggt
      961 cgaccctgac tacgtcgacc ggttggtcgc gctcggcatc ctgaccccag ctgccggaga
     1021 cgcgttctcg cccggtgatg tgctccgggc gcggtggctg cagagcctgg agcgagccgg
     1081 ggtgccgctg gagggactgg ccgccgccgt ccgggatggg gcgctgtcgc tctcgttcct
     1141 ggacgtggcc gcgtacgacc ggttcgccgg gctcagcggc accaccttcc accagctgag
     1201 tgcaagtacc gggatcccgc tggagctcct gatggtgctc cgcgaggccg tcggcttcgc
     1261 cgagccacgg ccagaggacc acgtccacca caacgagctg tcggtcgtgc cgctgatcca
     1321 gctgcagctt gccaaggggt tccaaccggt ggtcatcgag gggtggcttc gggtctacgg
     1381 ggacagcctg cgccgcatcg ccgaggccga ggcggcctgg tggaacagcg aggtcgagat
     1441 ggcgctgctg gcgtcgggca tgaccgaggg tgagatgctc cgggcccagg ccgacctcgg
     1501 ctcccagatg acccccctca tcgagcaggc cctgctggcc gtctaccacg gccagcagga
     1561 acacgcctgg agccaggtgt tcgtcgagca cgtcgagcgt gcgctggaag cggcgggcct
     1621 gtaccgccgg ttggaccgtc cgccggcaat gtgcttcctg gacatcaccg ggtacacgcg
     1681 cctgaccgag gagcggggcg accaggccgc cgccgacctg gcggcccggc tggctggcct
     1741 ggtccggcgg tcggcccagg agcatggggg gacaccggtg aagtggctcg gcgacggggt
     1801 gatgttctac ttccgggcgc ctccagctgg cgtgctggcc gccctggaga tggtggcggg
     1861 ggtcgggcgt cagggcctgc caccggccca tgtcgggatc cacgccggac cggtcatctt
     1921 ccaggacggt gactacttcg gccggaccgt caacctggcg gccaggatcg ccgagtacgc
     1981 ccgacccaac gaggtcctgg tcagccagga ggtcatcgac gcggcggacg gagcaccggt
     2041 gacgttcacc gacatcggac cggtcgagct gaagggcgtc ccaggcccgc tccgcctcta
     2101 caccgcccgc catgcctgaa aggcacgata tcggggctgt ccagagttct aatgtcctga
     2161 aggagcgggc ccagccggga gcgggcgtcg cgcctacgtc
//
LOCUS       DASSWR010000325         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_549853,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000325 DASSWR010000000
VERSION     DASSWR010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549853"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFO47_05430"
     CDS             complement(<1..1452)
                     /locus_tag="VFO47_05430"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8788169.1"
                     /translation="MVLQSRSPQRPGDLVVEVEEAGAEAVAGAVERARAAASGWAGGS
                     ALERSSALAAAADALAEAAGEVTDLIVREVGKPLTEAGAEVGRGVGILRYYAQQALDP
                     DGETYPGPSPAGLLLARRRPRGVAGLITPWNFPVAIPLWKAAPALAFGNAVVLKPSPE
                     ATATALRLAELLTPALGGDLFQVVPGGAGTGRALVDGADCVSFTGSVAVGRQVAVAAT
                     ERGVPSQAEMGGLNASIVLPDADPERAAAVVAGAAMGYAGQKCTATSRAIVVGDPRSF
                     TEALVAAVEGLAVGDPAEEATVVGPVITEPARRRVVEAAEEAAAGGGRLLTGGAAGDG
                     AGWFVAPTVLDGLGTEARLAQEEVFGPIAVVLAVADEDEAVRVANGVRYGLVGSVFTR
                     DLDRALGLAARLDTGMIRVNAPTSGVDYLAPFGGEKDSSYGPREQGKAARDFYTSTRT
                     ITILPAGGGAVALWQLLVDGRPRLARGPVDGPAE"
     gene            1619..2638
                     /locus_tag="VFO47_05435"
     CDS             1619..2638
                     /locus_tag="VFO47_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8788170.1"
                     /translation="MRDIAAAASVSQSTVSRVLSGAPTSVPIAPETRERVLQAARRLG
                     YRPNPLARGLRGASTNLIGAVVRDFSDPFFAGAVEALVVESMAHGYNVVLGHVDGRLD
                     EAVSLTSVLETRHTDAIVLLGDMQVQPRLLADLRSSSVPVVGLWQGTSPLEFPTADTD
                     DRAGIWAGLEHLESLGHRRIAFLSAELPVDYRVREDTYADWMGDRFGGVPPGYLERCP
                     NTLAGGDAALRRLLELPEPPTAVATSTDLVAVGVLHAAHSLGASVPGRLSVVGYDDIL
                     IASHTVPALTTLRMPIPEIVSHAVARAILLARDPEVPRGPSLEVFKPTLIVRDSTAPP
                     PAGAG"
     gene            complement(2642..>3033)
                     /locus_tag="VFO47_05440"
     CDS             complement(2642..>3033)
                     /locus_tag="VFO47_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HET8788171.1"
                     /translation="REGPVAAGAAEVTRRWAAGDRVVLDLDLPVRITEPHPRVDAVRG
                     CVAVERGPLVYCLEAADAPPGTELEELRWDPGRAPATVPRPDVGPGGVGVTVPVAGRG
                     AALSAGAVPYFAWANRGAGAMRVWIPR"
BASE COUNT          350 a         1235 c         1112 g          336 t
ORIGIN      
        1 ctcggccggc ccgtcgaccg ggccgcgggc cagccggggc cgcccgtcca ccaacagctg
       61 ccagagcgcc accgctccgc ccccggccgg gaggatggtg atggtccggg tcgaggtgta
      121 gaagtcgcgg gccgccttgc cctgctccct ggggccgtag ctggagtcct tctcgccccc
      181 gaacggggcc aggtagtcga cccccgaggt gggggcgttg acccggatca tgccggtgtc
      241 gagccgggcg gccaggccga gggcccggtc caggtcgcgg gtgaacaccg agccgaccag
      301 gccgtagcgg accccgttgg ccacccgcac cgcctcgtcc tcgtcggcca cggccagcac
      361 caccgcgatc ggcccgaaca cctcctcctg ggccaggcgg gcctcggtgc ccaggccgtc
      421 gagcacggtc ggggccacga accagccggc tccgtccccg gccgcgccgc cggtcagcag
      481 acggcccccg ccggccgcgg cctcctccgc ggcctccacc acccggcggc gggccggctc
      541 ggtgatgacc ggcccgacca cggtggcctc ctcggccggg tcgcccacgg ccaggccctc
      601 cacggccgcg accagcgcct cggtgaagga gcgggggtcg ccgaccacga tggcccggct
      661 ggtggcggtg cacttctggc cggcgtagcc catggccgcc ccggcgacca cggccgccgc
      721 ccgctcgggg tcggcgtcgg ggagcacgat cgaggcgttg aggccgccca tctcggcctg
      781 gctggggacg ccgcgctcgg tcgcggccac cgcgacctgg cggccgacgg ccaccgagcc
      841 ggtgaacgaa acgcagtcgg ccccgtccac cagcgcccgg ccggtcccgg ccccgccggg
      901 cacgacctgg aacaggtcgc ctccgagggc aggggtgagc agctcggcca ggcgcagggc
      961 ggtggcggtg gcctcgggcg acggcttgag cacgacggcg ttgccgaagg ccagggccgg
     1021 ggcggccttc cacagcggga tggccaccgg gaagttccac ggggtgatca gcccggccac
     1081 cccgcggggc cgccgccggg ccagcagcag cccggccggg ctcggtcccg ggtaggtctc
     1141 gccgtccggg tcgagggcct gctgggcgta gtagcgcagg atcccgaccc cgcggccgac
     1201 ctcggcgccg gcctcggtca gcggcttgcc gacctcccgg acgatcaggt cggtgacctc
     1261 cccggccgcc tcggcgaggg cgtcggcggc ggcggccagg gccgaggagc gctccagcgc
     1321 cgacccgccg gcccacccgg aggccgccgc ccgggcccgt tcgaccgccc cggcgacggc
     1381 ctccgcgccg gcctcctcga cctcgaccac caggtcgccg ggtcgctggg gggagcgact
     1441 ctgcagcacc accggcctcg cctccatcgc gtccgtcggt tcgatccgcc accctagcga
     1501 tgtgcccgcc cggcgtggcg gcgacacccg cccagctcca ccggagtgca atcgtattca
     1561 tgctagcctg gcggggatgt cgccccgcaa gcaccacccc gggtcgcctc cggcgacgat
     1621 gcgtgacatc gcggccgcgg cctcggtgtc gcagagcacc gtctcccggg tgctgagcgg
     1681 cgccccgacg agcgtgccga tcgcccccga gacccgcgag cgggtgctcc aggcggcgcg
     1741 gcggctcggc taccggccca accccctggc ccgggggctg cggggagcgt cgaccaacct
     1801 gatcggcgcc gtggtcaggg acttcagcga cccgttcttc gccggggcgg tcgaggccct
     1861 ggtggtcgag tcgatggccc acggctacaa cgtggtcctc ggccacgtcg acggccgcct
     1921 cgacgaggcc gtctcgctga cctcggtgct ggagacccgg cacaccgacg ccatcgtcct
     1981 gctcggcgac atgcaggtcc agccgcggct gctggccgac ctgcgcagct cgagcgtgcc
     2041 cgtggtcggc ctctggcagg ggaccagccc gctcgagttc cccacggccg acaccgacga
     2101 ccgggccggc atctgggccg ggctcgagca cctggagtcc ctcgggcacc ggcggatcgc
     2161 cttcctcagc gccgagctgc cggtcgacta ccgggtgcgg gaggacacct acgccgactg
     2221 gatgggcgac cgcttcggcg gggtcccgcc aggctacctg gagcggtgcc cgaacaccct
     2281 ggccgggggc gacgccgccc tgcggcgcct gctggagctg cccgagccgc cgacggccgt
     2341 ggccacctcc accgacctgg tcgccgtggg cgtcctgcac gccgcccaca gcctcggcgc
     2401 ctccgtgccc ggtcggctgt cggtggtggg ctacgacgac atcctgatcg ccagccacac
     2461 cgtgcccgcc ctgaccaccc tgcgcatgcc gatcccggag atcgtcagcc acgccgtggc
     2521 ccgcgcgatc ctcctggccc gcgacccgga ggtcccgcgc gggccgagcc tcgaggtctt
     2581 caagccgacc ctgatcgtgc gcgactcgac cgccccgccg ccggccggcg ccggctgacg
     2641 gtcagcgggg gatccagacc cgcatcgccc cggccccccg gttggcccag gcgaagtagg
     2701 ggaccgcgcc ggccgacagg gccgccccgc ggccggccac cggcacggtg accccgaccc
     2761 cgccggggcc gacgtccggg cgggggaccg tggccggggc ccgccccggg tcccagcgca
     2821 gctcctccag ctccgtcccg ggcggcgcgt cggccgcctc gaggcagtag acgagcgggc
     2881 cgcgctcgac ggcgacacag ccgcgcacgg cgtcgacccg cgggtggggc tcggtgatcc
     2941 ggaccggcag gtcgaggtcg agcacgaccc ggtccccggc cgcccagcgc cgggtcacct
     3001 cggccgcgcc ggccgccacc ggcccctcgc ggc
//
LOCUS       DASSWR010000326         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_944931,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000326 DASSWR010000000
VERSION     DASSWR010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944931"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFO47_05445"
     CDS             complement(<1..229)
                     /locus_tag="VFO47_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HET8788172.1"
                     /translation="MTQDRERLRSTFDQVADLYDRVRPGYPAALFDDLAELAGVGPGA
                     RVLEIGPGTGQATVPLAERGCRVVAVELGAGL"
     gene            complement(354..953)
                     /locus_tag="VFO47_05450"
     CDS             complement(354..953)
                     /locus_tag="VFO47_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788173.1"
                     /translation="MAVLFLLLAILCLVVLGAAGLENNDPSTATLLDRSFGQLSEGQL
                     LIVAAGLGFLVALFLFLAFGASRTRRTRRKELKSRRRDAEGRVDELERENARLRQELS
                     SSRDELGTVERNRDDAVAERDRAEATADRVRAAATPDRDRADTVATRDRTDATVPPEP
                     ASVRRERLDDRTGTTPVREEHARAADEHARAADEEVRRR"
     gene            complement(1054..1557)
                     /locus_tag="VFO47_05455"
     CDS             complement(1054..1557)
                     /locus_tag="VFO47_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HET8788174.1"
                     /translation="MSLYVGVAGASQAEAELLDQGELLGRRLAEGGAVVVCGGGPGVM
                     EAVCRGARSAGGITVGLLPGLDRADANRLLSVAVPTGMGQGRNLLLVRSSDALVAVGG
                     GFGTLSEIALALRTGVPVIGLATWSLHLDGQPVPAFPVAPDAETAARWALDAAGQVDP
                     ARGSGDG"
     gene            complement(1655..>3748)
                     /locus_tag="VFO47_05460"
     CDS             complement(1655..>3748)
                     /locus_tag="VFO47_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HET8788175.1"
                     /translation="VFDVLLGDGHRTALYLSPTKALARDQLRAIRSFALAGIRASAYD
                     GDTPSADRPMIRRSANLVLTNPDMLHRGILPGHERWAAFLGRLAFVVVDEAHTLRGVF
                     GSHVAVILRRLRRLAARYGASPTFVLASATLGNPAELASRLVGLPVEAVTEDGSPRGP
                     VAFALWEPPLLDETTGLRRSANTESAAWLAGAVEAGVRTLCFTRSRKAAELVATFARG
                     RVAQVDRRLAGRVRAYRAGYLAEERRELEQGLVSGLLLGLDTHNALELGMDVGGLDAV
                     VLDGYPGTVASMWQQAGRAGRQGEPSLAVLVGKDDPLDAYLLHHPADLFGRPHEAALV
                     DPTNPYVLAPHLLCAAYEGPLTDADLELFGGDSARALAGALEAEAALRRRGDKLHPGS
                     GRSQADQVDIRNAGGSPVTIVAADSGQVIGTVDRARAPSTVHRGAVYLHQGEHFKVAE
                     LDLGNGVALVTEAKGDEYTQARSDVDIAVVEPTRQVKLPRSTLWLGRVDVSEQVVGFE
                     RRRVGTGELLGHEELDLPPARLATVAYWYTLSPGLLDAAALGSRDIPGAAHAAEHAQI
                     GLLPLFAMCDRWDIGGLSTAWHPDTGAATIFVYDGYPGGAGIAEQGHRRALEHLQATR
                     DTVRSCPCETGCPSCVQSPKCGNGNNPLDKAGAVRLLDELLADLGAVPSPAGRVPGGR
                     GRGRGRGAVVRGGRG"
BASE COUNT          471 a         1344 c         1500 g          433 t
ORIGIN      
        1 ccaggccggc gccgagctcg acggccacca cccggcagcc gcgctcagcc agcgggaccg
       61 tcgcctggcc ggtgccgggg ccgatctcca gcacgcgggc acccggcccg acccccgcca
      121 gctcggccag gtcgtcgaac aacgccgccg ggtagccggg ccgcacccgg tcgtacagat
      181 cggcgacctg gtcgaaggtc gagcgcagcc gctcccggtc ctgggtcatg gctcgggtcc
      241 ctccgccata gggccgccac cgccacgggg aggggcccga gtcgttcagg gctcgggccc
      301 cagaaatccg ctcccagcca gcgactcctc gtcagccgcg gcgggtctcc tcatcagcgg
      361 cggcggacct cctcgtcagc ggcgcgggcg tgctcgtcgg ccgcgcgggc gtgctcctcg
      421 cgaaccgggg tggtcccggt gcggtcgtcg aggcgctccc gccggacgct ggccggttcg
      481 ggcgggacgg tggcgtcggt gcgatcccgg gtcgcgacgg tgtcggcgcg gtcgcggtcg
      541 ggggtggcgg cggcgcggac gcggtcggcg gtggcctcgg cgcggtcgcg ttcggcgacg
      601 gcgtcgtccc ggttgcgttc gaccgtgccg agctcgtcgc gggaggagga cagctcctgc
      661 cgcaggcggg cgttctcgcg ctcgagctcg tcgacccggc cctcggcgtc gcggcggcgc
      721 gacttgagct ccttgcgccg ggtgcgacgg gttctcgagg ccccgaaggc gaggaacagg
      781 aacagggcga ccaggaagcc gagcccggcg gccacgatca gcagctggcc ctcgctgagc
      841 tggccgaacg accggtcgag gagcgtggcc gttgacgggt cgttgttctc gagcccggcg
      901 gcgcccagca cgacaaggca gaggatggcg agcaacagga acaggacggc catttggcgc
      961 ctcctcaagg atcacgagtt cactggttcg atcgtcagcc tctagctgcc ccgtaactca
     1021 gctcatcaaa cggggcaagc tcgcgccggg agctcagccg tcgcccgacc cgcgggccgg
     1081 gtcgacctgg ccggcggcgt ccagggccca gcgggcggcg gtctcggcgt cgggggccac
     1141 ggggaacgcg ggaaccggct ggccgtcgag gtgcagggac caggtggcca ggccgatcac
     1201 cgggacgccg gtgcggaggg cgagggcgat ctcggagaga gtcccgaacc cgccgccgac
     1261 ggccaccagg gcgtcgctgg agcggaccag aagcaggttg cggccctggc ccatgccggt
     1321 ggggacggca acgctgagga ggcggttggc gtcggcgcgg tcgaggccgg gaaggaggcc
     1381 gaccgtgatc cccccggccg acctggcccc gcggcagacc gcctccatga cgccgggacc
     1441 gccgccgcac accaccaccg cgcccccctc ggccagcctc cggccgagga gctcgccctg
     1501 gtccagcagc tccgcctccg cctgtgacgc ccccgccacc ccgacgtaga ggctcacagc
     1561 cggccccgga gaccgccgcc cgccgggaac cccgtccgcg gcctcggggc aaccgaggcg
     1621 tcacgtgccg ggttccgcct tcccgccgag gccctcatcc tcgtccaccg cgaaccaccg
     1681 caccccgtcc ccgtccccga ccccggccgc cgggaacccg cccggccggc gacggaacgg
     1741 ccccgaggtc ggcgaggagc tcgtccagca ggcggaccgc gcccgccttg tccagggggt
     1801 tgttgccgtt gccgcacttg ggggactgga cgcaggaggg gcagccggtc tcgcaggggc
     1861 aggagcggac ggtgtcgcgg gtggcctgga ggtgctcgag ggcgcggcgg tgaccctgct
     1921 cggcgatgcc ggcgccgccc gggtagccgt cgtagacgaa gatcgtggcc gcgcccgtgt
     1981 ccgggtgcca ggcggtggag aggccaccga tgtcccagcg gtcgcacatg gcgaacaggg
     2041 gcagcagccc gatctgggcg tgctcggccg cgtgggcggc gccggggatg tccctggagc
     2101 cgagggcggc ggcgtccagc aggccggggg agagcgtgta ccagtaggcg acggtcgcca
     2161 gccgggccgg ggggaggtcg agctcctcgt ggccgagcag ctcccccgtg ccgacccggc
     2221 ggcgctcgaa cccgacgacc tgctcggaga cgtcgacccg gcccagccag agggtggagc
     2281 ggggcagctt cacctgccgg gtcggctcga cgacggcgat gtcgacgtcg ctccgggcct
     2341 gggtgtactc gtcgcccttg gcctcggtca ccagggcgac cccgttgccg aggtcgagct
     2401 cggcgacctt gaagtgttcg ccctggtgga ggtagacggc gccgcggtgc acggtcgacg
     2461 gcgcccgcgc ccggtcgacc gtgccgatga cctggccgga gtcggcggcg acgatggtga
     2521 ccgggctgcc gccggcgttg cggatgtcga cctggtcggc ctggctgcgg ccggagccgg
     2581 ggtgcagctt gtcgccccgg cggcgcagcg ccgcctcggc ctcgagggcg ccggccaggg
     2641 cccgcgcgct gtcgcccccg aacagctcca ggtcggcgtc ggtcaggggg ccctcgtagg
     2701 cggcgcagag cagatgcggg gcgagcacgt aggggttggt ggggtcgacc agggcggcct
     2761 cgtgggggcg gccgaacagg tcggcggggt ggtgcagcag gtaggcgtcg agcgggtcgt
     2821 ccttgccgac cagtacggcc agcgacggct cgccctggcg gccggcccgg ccggcctgct
     2881 gccacatcga ggcgaccgtg cccgggtagc cgtcgagcac gaccgcgtcc agcccgccga
     2941 cgtccatgcc cagctcgagg gcgttgtggg tgtcgagccc gagcagcagg cccgagacca
     3001 ggccctgctc cagctcgcga cgctcctcgg ccaggtagcc ggcccggtag gcgcggaccc
     3061 ggccggccag gcggcggtcg acctgggcga cccggccgcg ggcgaaggtg gccaccagct
     3121 cggccgcctt gcgcgagcgg gtgaagcaca gggtccggac cccggcctcg acggccccgg
     3181 ccagccaggc ggccgactcg gtgttggcgc tgcgccgcag cccggtggtc tcgtccagca
     3241 gcggcggctc ccagagggcg aaggcgaccg ggccgcgcgg ggagccgtcc tcggtgaccg
     3301 cctcgaccgg caggccgacc agccgggagg ccagctcggc cgggttgccg agggtggccg
     3361 aggcgagcac gaaggtcggg ctggccccgt agcgggcggc caggcggcgc aggcggcgca
     3421 ggatcacggc cacgtgcgag ccgaacaccc cgcgcagggt gtgggcctcg tcgacgacca
     3481 cgaaggcgag ccggccgagg aaggcggccc agcgctcgtg cccggggagg atgccgcggt
     3541 ggagcatgtc cgggttggtc agcaccaggt tggcgctgcg ccggatcatc ggccggtcgg
     3601 cggacggggt gtcgccgtcg taggcggacg cccggatccc ggccagggcg aacgaccgga
     3661 tggcgcggag ctggtcgcgg gccagggcct tggtggggga gaggtacagg gcggtgcggt
     3721 ggccgtcgcc gagcaggacg tcgaatac
//
LOCUS       DASSWR010000327         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1084269, whole genome shotgun sequence.
ACCESSION   DASSWR010000327 DASSWR010000000
VERSION     DASSWR010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084269"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFO47_05465"
     CDS             <1..404
                     /locus_tag="VFO47_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET8788176.1"
                     /translation="PDTSAARTTTTAAPTTTTRAPTTTVPPTTRRATEPTLRPAAGVT
                     VQVLNGVWVTGLARRVAGPVRSAGYEVVAAKTALGSYSVSRIYYTGGHRADAEAFKDR
                     FPAFSVVEPAPANLSRRVALHVVIGKDYPGV"
     gene            complement(522..1973)
                     /locus_tag="VFO47_05470"
     CDS             complement(522..1973)
                     /locus_tag="VFO47_05470"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET8788177.1"
                     /translation="MAELVMASNRGPVQFEVDDDGNLSAGRGAGGMVAALGPALAGQA
                     GTWVAAAITEGDRVAARRVARSGRLRTVELAQGDVRLRSLAFDPREYQSYYNRVANRT
                     IWFLHHHLFDVPRHPRFDRGFRVAWQHYEGVNQAFAGACDDEAGPGAEVFVQDYHLAL
                     VPAMLRRRRPDLRVAHFSHCPWADPGYFQMLPGRVAGAVLDGMLGADLLGFLVPRWAR
                     NFLHCCLDAGYRVDFTASTVQAADGRLVSVRTYPLGVDGDALRERAAQPDARAQDRVV
                     AELARGRSLIVRVDRMELSKNILRGLEGFGMFLERNPRARGRVVHYALTYPSRRDLPE
                     YQAYTAEVKHMADSINERYRTRTWEPVVLELRDNYPRALAAMAAADVLVVNPVWDGMN
                     LVAKEGPAISRRDLVLVLSRNAGAADDLGPGALLVNPFDTAELAETIAAALELSPDER
                     AKRAALLRDGATALPPQDWFLSQREDLAALRQP"
     gene            2078..>2489
                     /locus_tag="VFO47_05475"
     CDS             2078..>2489
                     /locus_tag="VFO47_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8788178.1"
                     /translation="MTQTGEEAARAATRAPLLEPPARRPSPYERFVRVGIVAWCGIGV
                     IILGYLMLRLMLFVNPVVPPLLIAVAVVYLLNPLVSALERRGVPRVAGAGIVYVLFLC
                     IVALVVSLLVPVVARQITQVIDHFPDYLADGQAYV"
BASE COUNT          328 a          908 c          924 g          329 t
ORIGIN      
        1 agccggacac cagtgctgcg cgaaccacga cgacggccgc gcccacgacc accacccggg
       61 cgccgacgac caccgtcccg ccgacgacca ggcgggccac cgagccgacc ctgcgcccgg
      121 ccgccggcgt caccgtgcag gtgctcaacg gcgtctgggt caccggcctg gcccgccggg
      181 tggccggccc ggtgcgctcg gccgggtacg aggtggtcgc ggccaagacc gccctcggca
      241 gctactcggt ctcccgcatc tactacacgg gcggccaccg ggccgacgcc gaggcgttca
      301 aggaccgctt cccggccttc tcggtggtcg agccggcccc ggccaacctg tcgcgccggg
      361 tcgccctgca cgtggtcatc gggaaggact acccgggagt ctgacctcgg cccggaccag
      421 gccggtggcg gcgcggaagg cccggatgag gtggctcggg ctccagccgg ccacgccggc
      481 cagggagcga gtcccccaca tggatcaggg accgcggctg gtcagggttg ccggagagct
      541 gcaaggtcct ccctctggga gagaaaccag tcctgggggg gcagggcggt ggccccgtcg
      601 cggaggaggg cggcgcgctt ggcccgctcg tcgggggaga gctcgagggc ggcggcgatg
      661 gtctcggcca gctcagcggt gtcgaagggg ttgaccagca gggcgcccgg gccgaggtcg
      721 tcggcggcgc cggcgttgcg ggacaggacc aggaccaggt cgcggcggct gatcgccggg
      781 ccctccttgg cgaccaggtt catgccgtcc cacaccgggt tgacgaccag cacgtcggcg
      841 gcggccatgg cggccagggc ccgcgggtag ttgtcgcgca gctccagcac caccggctcc
      901 caggtccggg tccggtagcg ctcgttgatg gagtcggcca tgtgcttcac ctcggccgtg
      961 tacgcctggt actcgggcag gtcgcgccgg gagggatagg tgagggcgta gtggaccacc
     1021 cgcccccggg ctctggggtt ccgctccagg aacatgccaa agccctccag cccgcgcagg
     1081 atgttcttgg acagctccat gcggtcgacc cgcacgatca ggctgcggcc cctggccagc
     1141 tcggccacca cccggtcctg ggcgcgggcg tcgggctggg cggcccgctc ccggagggcg
     1201 tcgccgtcca cccccagcgg gtaggtgcgc accgacacca ggcggccgtc ggcggcctgg
     1261 accgtgctgg cggtgaagtc gacccggtag ccggcgtcca ggcagcagtg gaggaagttg
     1321 cgggcccagc gggggaccag gaacccgagc aggtcggccc cgagcatccc gtcgaggacc
     1381 gcgccggcca cccgcccggg cagcatctgg aagtagccgg ggtcggccca ggggcagtgg
     1441 ctgaagtggg cgacgcgcag gtcgggccgg cgccggcgca gcatcgccgg gaccagggcc
     1501 aggtggtagt cctgcacgaa cacctcggcc cccggaccgg cctcgtcgtc gcaggcgccg
     1561 gcgaaggcct ggttgacgcc ctcgtagtgc tgccaggcga cccggaagcc gcggtcgaag
     1621 cgggggtggc ggggcacgtc gaacaggtgg tggtgcagga accagatggt ccggttggcg
     1681 acccggttgt agtagctctg gtactcccgc ggatcaaagg ccaggctgcg caggcgcacg
     1741 tccccctggg ccagctcgac ggtgcgcagc cgccccgagc gggcgacccg ccgggccgcc
     1801 acccggtccc cctcggtgat ggcggccgcg acccaggtgc cggcctggcc ggccagggcc
     1861 gggccgaggg cggccaccat gccgccggcg ccccggccgg cgctcaggtt gccgtcgtcg
     1921 tccacctcga actggaccgg accgcggttg gacgccatca ccagctcggc catcatcgca
     1981 acaccagcct ttcggcgtca ccaggaacca ctatgctcta agagcatcct ggcattcctg
     2041 aacgcgacgg tcgaccggat ccgaaggcgg cgaggcattg acccagacgg gcgaggaggc
     2101 ggcccgggcg gcgacgcggg cgcccctgct cgagccgccg gcgcgccgcc cctccccgta
     2161 cgagcggttc gtgcgcgtcg gcatcgtggc ctggtgcggc atcggcgtca tcatcctcgg
     2221 ctacctgatg ctgcggctga tgctgttcgt caacccggtg gtgccgcccc tgctgatcgc
     2281 cgtggccgtc gtctacctgc tcaacccgct cgtgtcggcc ctggagcgcc gtggcgtgcc
     2341 ccgggtggcc ggcgccggca tcgtctacgt cctgttcctg tgcatcgtcg cgctggtcgt
     2401 gtccctgctc gtccccgtgg tggccaggca gatcacccag gtgatcgacc acttccccga
     2461 ctacctggcc gacggccagg cctacgtcc
//
LOCUS       DASSWR010000328         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_578054,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000328 DASSWR010000000
VERSION     DASSWR010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578054"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..962
                     /locus_tag="VFO47_05480"
     CDS             198..962
                     /locus_tag="VFO47_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit A"
                     /protein_id="HET8788179.1"
                     /translation="MAVDSKTLELRALKDKQYRWIDRKWDAVFWITAAFVVGAAADIT
                     KQLFAGDWDFWTDWKDRQWWPIITPFAIIIIGSALQYIQWLAWRFPTGMTYTAVCMFL
                     CTLIGRWLQWGAFVYYPMNFVWPATMVAAAIFADWVLLKTKSFVLTSVIGGILFAVTW
                     WISNYIPMAPFLQPAQWMDRVVTVADIQGIEYVRSQTPEYLRIIESGSLRTFLGETQY
                     VSLVFGATVAVAGYWVGQFIGRGLAIWPIGRFMKKW"
     gene            962..2194
                     /locus_tag="VFO47_05485"
     CDS             962..2194
                     /locus_tag="VFO47_05485"
                     /inference="COORDINATES: protein motif:HMM:NF016619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HET8788180.1"
                     /translation="MTMRRRIRRVFWATLVAGALLVGSAPAASAHGERAQEAFLRMRT
                     VAWIDTTYSRTTVRQGETFTITGTAKILDAWPTKLAAGAPNTGFMGIIAPGPVVLLKE
                     RTINGVSAPTRIDIRRGGIYEFSMTVAGRRPGRWHVHPSFSVKGAGTLLGPGEWITVE
                     ENPDGFTNDVQLVGGGTVNLENYQLTPVWIWLILTFLIGLAWMIYWTVPKRTVTNLAV
                     TSQIPLNTDGMEYGLITKKDHRNMNLFVIGTVLLLVVGWIWQSAAFPDKMPQQIIQFA
                     PPEPAIASAPALAEAAVGSATYDAASQTLTVQTDITNLSQEPVNLTQFTTTTLRFTTG
                     SAAGPGVLQVAPDSTIQPGSAPTRVTLTMKDPAWETEHLVPIGESQLLVTGVMVFETA
                     DGQKNYTELEANLTPRFD"
     gene            2207..2359
                     /locus_tag="VFO47_05490"
     CDS             2207..2359
                     /locus_tag="VFO47_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788181.1"
                     /translation="MPGYTVAHMGGIDELAIIWMPLVMGIGLWLIFRTGKDETSQEDA
                     EVTEEQ"
     gene            2466..2741
                     /locus_tag="VFO47_05495"
     CDS             2466..2741
                     /locus_tag="VFO47_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788182.1"
                     /translation="MSQLLAHGGMFAASNSLLVFLPFIVAGAGLAFIIRAFSDKIDRK
                     PDDGVARLPTLEDRPLYHVHMQIRSGSKPSSASRAAGPTVAKLPARR"
BASE COUNT          488 a         1036 c         1023 g          480 t
ORIGIN      
        1 tggctggcgg ccgggatctt cgccctctgg gccgagacgc tgatccgcct gctccagatc
       61 gagggcgaga tcgaggcgga ggcagggcag cccgcggaag aggtcgccta ggagccgcca
      121 gggctcttcc ggcatagcgc acgtcccgcc ggggcgcacc cgccgcggcg gtcgggtcga
      181 ggacgggagg gtgggccgtg gccgttgact ccaagacgct ggagctgcgc gcgctcaagg
      241 acaagcagta ccggtggatc gaccgcaagt gggacgcggt cttctggatc acggccgcct
      301 tcgtggtggg cgcggccgcc gacatcacca agcagctgtt cgcgggcgac tgggacttct
      361 ggaccgactg gaaggaccgg cagtggtggc cgatcatcac gccgttcgcg atcatcatca
      421 tcggctcggc cctgcagtac atccagtggc tggcctggcg cttccccaca gggatgacct
      481 acacggcggt gtgcatgttc ctgtgcacgc tgatcggccg ctggctgcag tggggcgcct
      541 tcgtctacta cccgatgaac ttcgtctggc cggcgacgat ggtggcggcg gcgatcttcg
      601 ccgactgggt gttgctgaaa accaagagct tcgtgctcac ctcggtgatc ggcggcatct
      661 tgttcgcggt cacctggtgg atatccaact acatcccgat ggcgcccttc ctccaaccgg
      721 cccagtggat ggaccgggtg gtgacggtcg ccgacatcca ggggatcgag tacgtccgca
      781 gccagacccc cgagtacctc cggatcatcg agtcaggctc gctgcggacc ttcctcggtg
      841 agacgcagta cgtctcgctg gtcttcgggg cgacggtcgc cgtcgccggg tactgggtcg
      901 ggcagttcat cgggcgcggt ctggccatct ggccgatcgg ccgcttcatg aagaagtggt
      961 gatgacgatg cgacgccgaa tccgccgggt cttctgggcc accctcgtgg ccggggcgct
     1021 gctggtcggg tcagccccgg ccgcctccgc ccacggcgag cgggcccagg aggcgttcct
     1081 gcgcatgcgc acggtggcct ggatcgacac cacctactcc aggaccacgg tcaggcaggg
     1141 cgagaccttc accatcaccg gcacggccaa gatcctggac gcctggccga ccaagctggc
     1201 cgccggcgcg cccaacaccg gcttcatggg gatcatcgcc cccggcccgg tggtgctgct
     1261 gaaggagcgg accatcaacg gcgtgtcggc gccgacccgc atcgacatcc gccgcggtgg
     1321 catctacgag ttcagcatga ccgtggccgg gcgccgcccc ggcaggtggc acgtgcaccc
     1381 gtccttctcg gtcaagggcg ccggcaccct gctcggcccc ggcgagtgga tcaccgtcga
     1441 ggagaacccc gacggcttca ccaacgacgt gcagctggtc ggcgggggca ccgtcaacct
     1501 cgagaactac cagctcacgc ccgtctggat ctggctgatc ctgaccttcc tgatcggcct
     1561 ggcctggatg atctactgga cggtccccaa gcggacggtg accaacctgg ccgtgaccag
     1621 ccagatcccg ctcaatacgg acgggatgga gtacgggctg atcaccaaga aagaccatcg
     1681 caacatgaac ctgttcgtca tcgggacggt gctgctgctg gtggtcggct ggatatggca
     1741 gtcggcggcc ttcccggaca agatgcccca gcagatcatc cagttcgctc cgcccgagcc
     1801 ggccatcgcc agcgcgcccg cgctggccga ggcggcggtg ggctcggcca cctacgacgc
     1861 cgccagccag accctgaccg tccagacgga catcaccaac ctcagccagg agcccgtcaa
     1921 cctgacccag ttcacgacca ccaccctcag gttcaccacc gggtcggcgg cggggccggg
     1981 ggttctgcag gtcgcacccg attcgacgat ccaaccgggg tcggcgccga ccagggtcac
     2041 cctgaccatg aaggatcccg cctgggagac cgagcacctc gtcccgatcg gcgagtcgca
     2101 gctgctggtc accggggtca tggtgttcga gacggccgac ggccagaaga actacaccga
     2161 gctggaagcc aacctcaccc cacgattcga ctagttacgg agcggcatgc cgggttacac
     2221 ggttgcgcac atgggaggga tcgacgagct cgccatcatc tggatgccgc tggtgatggg
     2281 gatcggcctg tggctgatct tccgcaccgg caaggacgag acgagccagg aggacgcgga
     2341 ggtgacggag gagcaatgac catcctcttc gtggccgcgc cgatcgtgat ggccatcggg
     2401 ctgtatctga tcctgcgctt caccaaggag ccaggggcgg acgacccagg gatgccggag
     2461 gagcgatgag ccagctgctc gcccacggcg gcatgttcgc ggccagcaac agcctgctgg
     2521 tgttcctgcc gttcatcgtc gccggggcgg gcctggcctt catcatccgg gccttcagcg
     2581 acaagatcga ccgcaagccc gacgacggcg tggcccggct gcccacgctg gaggaccggc
     2641 ctctgtacca cgtccacatg cagatccgtt ccgggagcaa gccgtccagc gcgtcgcggg
     2701 cggccgggcc gacggtggcc aagctcccgg cacggcgatg agcctcgaga caggagcggg
     2761 acgatggcgg gaatcctgtt cgcagtcctg atcttccttc tgacgatcgg ctgcatcggc
     2821 gtcgtcatct ggggctcgga gccgccctcc tgacggcgac gccacccggt ccgcgaccgg
     2881 tcgcccccct cgaacgcacc ctcgagcggg gcatcatgac cggcatggtc gcggccgcgg
     2941 tcatggccct gttctccatg gtcgccgcgg ccgccgacct cggcgtgtac ggccggctgt
     3001 acctggtcac ggccatcgtc gagccgg
//
LOCUS       DASSWR010000329         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_95436,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000329 DASSWR010000000
VERSION     DASSWR010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95436"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VFO47_05500"
     CDS             complement(<1..1357)
                     /locus_tag="VFO47_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET8788183.1"
                     /translation="MTTADGWVCVDGNEAAARVAHRLSEVIAIYPITPASTMGELADT
                     WSAAGRPNLWGQVPEVVELQSEAGAAGVLHGAVLKGALATTFTASQGLLLMLPNMFKI
                     AGELTPTVIHVAARTVATHALSIFGDHSDVMAARPTGWAMLAAGSVQEAHDLALVAHA
                     SALASRVPFLHFFDGFRTSHEVAKIRALDDDDLRSLVDEADVAAHRDRGLSPDRPQLR
                     GSAQNPDVFFQAREASNPFYAAVPGIVQAKLDGLAARTGRRYGLVDYLGAPDAERVLV
                     LMGSAAGAAAEAVEALTAAGERVGLVTVRLYRPFPAEALAAALPATVRRVAVLDRTKE
                     PGAAGEPLYQDVVCALAERAAGGGPPMPAVVGGRYGLASKEFTPAMARAVLDNLAAEA
                     PRNHFTVGIADDVSRTSLPVDGSFSTEDPEGLRAVFFGLGSDGTVGANKTSVKIIGEG
                     TD"
     gene            complement(1448..1840)
                     /locus_tag="VFO47_05505"
     CDS             complement(1448..1840)
                     /locus_tag="VFO47_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8788184.1"
                     /translation="MKVSDLQPHELFTAQADESLDVAADRMNWHQVGALPVLEGQHLV
                     GIITERDVTAAMAEGADPVITPVSDYMTPAPEVLRPDSDLADAAHLMLELGIRHLPIV
                     RSGHLVGVLSIRDVLDAEVHHADGSRAG"
     gene            complement(1930..>2337)
                     /locus_tag="VFO47_05510"
     CDS             complement(1930..>2337)
                     /locus_tag="VFO47_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET8788185.1"
                     /translation="RTLHPQTRENLLRAMRREAYAFAMYLLYATQARKHGRPEIAQLF
                     EQVAKEEYFGHFVEQALEVGVIGMEAENLHLAIEEEAFEAEVMYRDFQVQAEAVGDQE
                     AAERFRRLREDERRNRDQFLAVLRNLDPADPAE"
BASE COUNT          299 a          852 c          849 g          337 t
ORIGIN      
        1 ggtcggtgcc ctcgccgatg atcttcaccg aggtcttgtt ggccccaacg gtcccgtcgg
       61 agccgaggcc gaagaacacc gcccgtaggc cctcggggtc ctcggtggag aagctgccgt
      121 cgaccggcag gctggtccgg gagacgtcgt cggcgatccc gacggtgaag tggttgcgcg
      181 gcgcctcggc ggccaggttg tccagcaccg ccctggccat ggccggggtg aactccttgg
      241 acgccaggcc gtagcggccc cccaccaccg ccggcattgg cgggccgccg cccgccgccc
      301 gctcggccag ggcacacacc acgtcctggt acaggggctc gccggccgcg cccggctcct
      361 tggtccggtc cagcaccgcc acccggcgca cggtcgcggg cagggcggcg gccagggcct
      421 cggccgggaa cggccggtac agccgcaccg tgaccaggcc gacccgctcc cccgccgccg
      481 tcagcgcctc gaccgcctcg gcggcggcac cggcggccga ccccatgagc accagcaccc
      541 gctcggcgtc gggggcgccg aggtagtcga ccagcccgta gcggcggccg gtgcgggcgg
      601 ccagcccgtc cagcttggcc tgcacgatcc cgggcacggc ggcgtagaag gggttgctgg
      661 cctcccgggc ctggaagaag acgtccgggt tctgggccga gccgcgcagc tggggccggt
      721 ccggggacag gccccggtcg cggtgggcgg ccacatcggc ctcgtcgacc agggatcgga
      781 ggtcgtcgtc gtccagggcc cggatcttgg ccacctcgtg ggaggtgcgg aagccgtcga
      841 agaagtgcag gaacggcacc cgcgaggcca gcgccgaggc gtgggcgacc agggccaggt
      901 cgtgggcctc ctgcaccgag ccggcggcca gcatcgccca gccggtgggg cgggcggcca
      961 tcacgtcgga gtggtcgccg aagatcgaca gggcgtgggt ggccaccgtg cgggcggcca
     1021 cgtggatcac cgtcggggtc agctccccgg cgatcttgaa catgttgggc agcatcagca
     1081 gcaggccctg ggaggcggtg aacgtggtgg ccagcgcccc cttgagcacc gccccgtgca
     1141 gcaccccggc cgccccggcc tcgctctgga gctcgaccac ctcgggcacc tgcccccaca
     1201 ggttgggccg cccggccgcg ctccaggtgt cggccagctc ccccatcgtc gacgccggcg
     1261 tgatcgggta gatggcgatg acctcgctca gccggtgggc gacccgcgcc gccgcctcgt
     1321 tgccgtcgac acacacccaa ccgtccgcgg tcgtcatcac agcctccgag ttcgtgcttg
     1381 gtcggtgcca tccgaccaag tgtcccgcct gcggcggcag ggtccaaggg cctgccggga
     1441 acgggaccta tccggctcgg ctcccgtcgg catggtggac ctcggcgtcc aggacgtcgc
     1501 ggatggacag gacaccgacc aggtggccgc tgcgcacgat gggcaggtgc cggatgccca
     1561 gctccagcat cagatgggcg gcgtcggcga ggtcgctgtc gggccgcagg acctccgggg
     1621 ccggggtcat gtagtccgac accggcgtga tgaccgggtc cgcgccctcg gccatcgcgg
     1681 ccgtgacgtc ccgttcggtg atgatgccga ccagatgctg gccctccagg accggaaggg
     1741 cgccgacctg atgccagttc atgcggtcgg ccgcgacgtc caggctctcg tccgcctggg
     1801 cggtgaacag ctcgtgaggc tgcaggtcgg agaccttcat cggtgcctcc tttgtcgttg
     1861 ccctggcacc gtagccgagc gctcggtccg tccctagggc ccctggtccc gattctgggg
     1921 ggccgttcgc tactcggccg ggtcggctgg gtccaggttc cgcaggacgg ccaggaactg
     1981 gtcacggttg cgccgttcgt cctcgcgcag gcgccggaac cgctcggcgg cctcctggtc
     2041 gccgaccgcc tcggcctgga cctggaagtc gcggtacatc acctcggcct cgaaggcctc
     2101 ctcctcgatg gccaggtgca ggttctcggc ctccatgccg atgaccccca cttccagggc
     2161 ctgctcgacg aagtggccga agtactcctc cttggccacc tgctcgaaca gttgggcgat
     2221 ctccggtcgc ccgtgcttgc gggcctgggt ggcatacagc aggtacatgg cgaaggcgta
     2281 ggcctcccgc cgcatcgccc ggagcaggtt ctcccgggtc tgggggtgca gggtccg
//
LOCUS       DASSWR010000330         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_40883,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000330 DASSWR010000000
VERSION     DASSWR010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40883"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..655
                     /locus_tag="VFO47_05515"
     CDS             47..655
                     /locus_tag="VFO47_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8788186.1"
                     /translation="MLDLEVIDNPAAAVVALDPLRARLLAGLAEPASASALAERFALP
                     RQKISYHLRILEEHGLVAEVGRRRWGGLTERVLHATAAAYVVSPGALGEAAGDPERIA
                     DRLSARYLIALAARAVREVAALARRADQAGRRLPTLALDTEIRFRSAAERAAFTSDLA
                     AAVTTLVSRYHDAGAPGGRSHRLVVVAHPLPGGPRPDTEEAS"
     gene            652..1470
                     /locus_tag="VFO47_05520"
     CDS             652..1470
                     /locus_tag="VFO47_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET8788187.1"
                     /translation="MSSDADNTPGTRAIDLDVEVPGTPEEVWEAVASGPGITAWFVPA
                     KVDGRVGGTLELDFGPLYGSETARITAWDPPRRFVAETAHEGRPTFATEWLVEARDGG
                     TCVVRLIQSGFGSGAEWDAEYDATEAGWKLFLFNLRLYLTHFPGQPCASLLVNGKAAG
                     PVDRAFGELAGALGLPAGARAGERVAATAADAPPLAGVVERAEPNMLTLLLEKPAPGV
                     AFVVAEPAADGSAHASIYAYLFGEGAAEVAAREELAVRHWMQRRFPLPEAPPGA"
     gene            complement(1486..2280)
                     /locus_tag="VFO47_05525"
     CDS             complement(1486..2280)
                     /locus_tag="VFO47_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788188.1"
                     /translation="MIPAPPSDRGARREPPGPPALDVDGVAYAYPDGHQALFGVSFRI
                     ERGERVALLGPNGAGKTTLVLQCNGVLLPGAGRVTVAGLPVAAEHLREIRRRVGIVFQ
                     DPDDQLFMPTVAEDVAFGPANLGLRGPELETRVKAALAAVGMEEHAARPPHHLSFGQR
                     RRVAVATVLAMEPEVLVLDEPSSNLDPASRRELAEIVTGLDITTLMVTHDLPYALQLC
                     PRSLIMNGGTITADGPTPDLLADTDLLAANRLELPFGFDPSALRGR"
     gene            complement(2277..>2704)
                     /locus_tag="VFO47_05530"
     CDS             complement(2277..>2704)
                     /locus_tag="VFO47_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HET8788189.1"
                     /translation="KGTLGVATSVILAATTPVPELLRGLERLRLPAAFTTIAGFMVRY
                     GDVIADEVRRMRIARVSRGHDPRWIWQARAVAASAGTLFIRSYERGERVYLAMVSRGY
                     AGSMPVLEDLGATRAQWLAALALPAAAALVAVTAWGVRP"
BASE COUNT          338 a         1031 c         1017 g          318 t
ORIGIN      
        1 ggagcacccc ttgacaaaat ttcttttcaa tggtgccatg gcccgcatgc tggacctcga
       61 ggtgatcgac aacccggccg ccgccgtggt ggccctcgac ccgctccggg cccgcctgct
      121 ggccgggctg gccgagccgg cctcggcctc ggccctggcc gagcggttcg ccctcccccg
      181 ccagaagatc agctaccacc tgcgcatcct ggaggagcac ggcctggtcg ccgaggtcgg
      241 ccggcgccgc tggggcggcc tgaccgagcg ggtcctgcac gcgaccgcgg ccgcctacgt
      301 ggtctccccc ggcgccctcg gcgaggccgc cggcgacccc gaacggatcg ccgaccggct
      361 gtccgcccgc tacctgatcg ccctggccgc ccgggccgtg cgcgaggtcg ccgcactggc
      421 ccgccgggcc gaccaggccg gcaggcggct gcccaccctg gccctcgaca ccgagatccg
      481 cttccgctcc gccgccgagc gggccgcgtt caccagcgac ctggccgccg ccgtgaccac
      541 cctggtctcg cgctaccacg acgccggcgc ccccggcggc cgctcccacc gcctggtggt
      601 ggtcgcccat cccctgcccg gcggtccccg acccgacacc gaggaggcat catgagcagc
      661 gacgcagaca acacccccgg gaccagggcg atcgacctgg acgtcgaggt ccccgggacg
      721 cccgaggagg tctgggaggc ggtcgccagc ggtcccggga tcaccgcctg gttcgtcccg
      781 gccaaggtcg acggccgcgt gggcgggacc ctcgagctcg acttcggacc cctctacggg
      841 tcggagacgg cccggatcac ggcctgggac ccgccgcgcc ggttcgtggc cgagacggcc
      901 cacgagggcc gccccacctt cgccaccgag tggctggtgg aggcccgcga cggcggcacc
      961 tgcgtggtcc ggctgatcca gagcggcttc ggcagcggcg ccgagtggga cgccgagtac
     1021 gacgccaccg aggccgggtg gaagctgttc ctcttcaacc tgcgcctgta cctgacccac
     1081 ttccccggcc agccctgcgc ctcgctgctg gtcaacggca aggccgccgg gccggtggac
     1141 cgggccttcg gcgagctcgc cggcgccctc ggcctgcccg ccggggccag ggcgggcgag
     1201 cgggtggcgg cgaccgccgc ggacgcgccg ccgctggccg gggtggtcga gcgggccgag
     1261 cccaacatgc tcaccctgct gctggagaag ccggcccccg gcgtcgcctt cgtggtcgcc
     1321 gagcccgccg ccgacgggag cgcccacgcc agcatctacg cctacctgtt cggcgagggg
     1381 gcggcggagg tggcggccag ggaggaactc gccgtgcggc actggatgca gcgccgcttc
     1441 cccctgcccg aggcgcctcc aggggcctga gctggcgcgc ccgggtcagc ggccgcgcag
     1501 ggccgaggga tcgaagccga aggggagctc gaggcggttg gcggccagca ggtcggtgtc
     1561 tgccagcagg tcgggcgtgg gcccgtcggc ggtgatggtg ccgccgttca tgatcaggga
     1621 gcgggggcag agctggaggg cgtagggcag gtcgtgggtg accatcaggg tggtgatgtc
     1681 caggccggtg acgatctcgg ccagctcgcg gcggctggcc gggtcgaggt tggacgacgg
     1741 ctcgtccagg accaggacct ccggctccat ggcgagcacg gtggccaccg cgacccgccg
     1801 ccgctgcccg aacgacaggt ggtggggcgg gcgggcggcg tgctcctcca tgcccaccgc
     1861 ggccagggcg gccttcaccc ttgtctccag ctcgggcccg cgcaggccca ggttggccgg
     1921 cccgaaggcc acgtcctcgg ccaccgtcgg catgaagagc tggtcgtcgg ggtcctggaa
     1981 gacgatcccg acccggcgcc ggatctccct gaggtgctcg gcggccaccg gcagcccggc
     2041 caccgtgacc cggcccgcac ccggcaggag cacgccgttg cactgcagca ccagggtggt
     2101 cttgccggcc ccgttgggcc ccagcagggc cacccgctcc ccccgctcga tccggaagct
     2161 gaccccgaac agcgcctggt gcccgtccgg gtaggcgtag gcgaccccgt cgacgtcgag
     2221 ggcggggggt ccggggggct cacgcctcgc cccccggtcg gaaggcggcg ccgggatcac
     2281 gggcggacgc cccaggcggt gacggcgacc agggcggcgg cggccggcag ggccagggcg
     2341 gccagccact gtgcccgggt cgcgcccagg tcctccagca ccggcatcga cccggcgtag
     2401 ccgcgcgaca ccatcgccag gtagacccgc tcgccgcgct cgtaggagcg gatgaacagc
     2461 gtcccggcag aggcggcgac ggccctggcc tgccagatcc agcgcgggtc gtggccgcgg
     2521 gagacgcggg cgatgcgcat gcgccggacc tcgtcggcga tcacgtcgcc gtagcggacc
     2581 atgaacccgg cgatggtggt gaaggccgcc ggcaggcgca gccgctccag cccgcgcagc
     2641 agctcgggca cgggcgtggt ggcggccagg atgaccgagg tggccacccc gagggtgccc
     2701 ttga
//
LOCUS       DASSWR010000331         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1228404, whole genome shotgun sequence.
ACCESSION   DASSWR010000331 DASSWR010000000
VERSION     DASSWR010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228404"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1034)
                     /gene="lipA"
                     /locus_tag="VFO47_05535"
     CDS             complement(48..1034)
                     /gene="lipA"
                     /locus_tag="VFO47_05535"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET8788190.1"
                     /translation="MPEPVIPPGVRTLGVLPRTPAPPPTGATFARKPAWLKVKASQGP
                     NYRELKGLVRDEQLNTVCEQAGCPNIYECWEAREATFLIGGDHCTRACPFCQIHSGKP
                     EGYDTDEPRRVAHAIGRMGLRFAVITGVARDDLPDGGAWLFAESVRQVRALLPSCGVE
                     VLIPDFRGQPEALRQVTEVEPDVLGHNVETVPRLYKRIRPGFTYAGSLELLARGRAWL
                     PEGCATKSNLILGMGEERDEVLQTMADLRSVGVELLTVTQYLQPTKAHLNLQRFVPPE
                     EFADLRAHAEGLGFAHVESGALVRSSYHAGEMHKAAVRKQRGQLPAWATADA"
     gene            complement(1027..1734)
                     /gene="lipB"
                     /locus_tag="VFO47_05540"
     CDS             complement(1027..1734)
                     /gene="lipB"
                     /locus_tag="VFO47_05540"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET8788191.1"
                     /translation="MRELWTVRAGTVPYRRAWAWQRALVERRAAGDIPDTVLLLEHPH
                     VYTIGKRGSDADVLADPAWLAAQGAEVVRSDRGGQVTYHGPGQLVGYPITLLDPSPDV
                     WRFVGRVEQALVDVAAGFGLEARGERGDLTGVWVGDAKLGAIGMRVSRGVTSHGFALN
                     CATDLAFFNAIVPCGMPEVAACSLSSLLGRQVPVAEVLPAMERHLAGRLERTPVAVDP
                     ATLDLPASDHAPETVHA"
     gene            complement(1738..>2823)
                     /locus_tag="VFO47_05545"
     CDS             complement(1738..>2823)
                     /locus_tag="VFO47_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET8788192.1"
                     /translation="EAEEQPAKEEPKPKEPKPKEDKPKEDKPAAKAKGDGKGNGKAAA
                     GVSPLVRRLAREHDVDLSQVQGSGSGGRVRREDVEAFVQQRGKEPAAAARAEPKGKAA
                     AAAPSASPQVAVPLAKGLREEVVPATRLRKVIAERMVASLQTSAQLTSAVEIDMTRVM
                     RLRERAKEEFKQREGVSLSPMPFAVLALVAAIREHPTVNSAMDEDGNLHVYQDVHLGV
                     AVDTPKGLFVPVIKNAESLNLAGLAKAIADVAARTRDGKIDPGELSGSTISLTNTGSR
                     GAVWDTPIINQPNAAIFATPAIVKRPVVVEDPDLGEVVAVRHMMYGILTYDHRIIDGA
                     DAARFLVKVKEVLEAADFEGDLGLERE"
BASE COUNT          341 a         1003 c         1072 g          407 t
ORIGIN      
        1 ggcgacggcc agcagcgcga gcaggaccag gcgggcgcgg ggcacggtca ggcgtcggcg
       61 gtggcccagg cggggagctg gccgcgctgc ttgcggacgg cggccttgtg catctcgccg
      121 gcgtggtagc tggagcggac cagggccccc gactccacgt gggcgaaccc gaggccttcg
      181 gcgtgggccc tgaggtcggc gaattcctct ggggggacga accgctgcag gttcaggtgg
      241 gccttggtgg gctgcaggta ctgggtgacg gtgagcagct cgaccccgac cgagcgcagg
      301 tcggccatgg tctggaggac ctcgtcgcgc tcctcgccca tgcccaggat caggttcgac
      361 ttggtcgcgc agccctcggg cagccatgcc ctgcccctgg ccagcagctc cagcgacccg
      421 gcgtaggtga acccgggccg gatccgcttg tacagccggg gcacggtctc gacgttgtgg
      481 ccgagcacgt ccggctcgac ctcggtgacc tggcgcagcg cctcgggctg gccgcggaag
      541 tcggggatca gcacctcgac gccacacgaa ggcagcagcg cccgcacctg gcggaccgac
      601 tcggcgaaca gccaggcccc gccgtcgggc aggtcgtcac gggccacccc ggtgatgacg
      661 gcgaaccgca gccccatgcg gccgatggcg tgggccaccc ggcggggctc gtcggtgtcg
      721 tagccctcgg gcttgccgga gtggatctgg cagaacgggc aggcccgggt gcagtggtcc
      781 ccgccgatca ggaaggtagc ctcccgggcc tcccagcact cgtagatgtt ggggcagccg
      841 gcctgctcgc agacggtgtt gagctgctcg tcgcggacca ggcccttgag ctcgcggtag
      901 ttcgggccct gggaggcctt caccttcagc caggccggct tgcgggcgaa ggtcgccccg
      961 gtcggcggcg gcgccggggt gcggggcagc acccccaggg tgcggacccc gggcgggatc
     1021 acgggctcag gcatggacgg tctccggggc gtggtcggac gccggcaggt ccagggtagc
     1081 ggggtcgacc gcgaccgggg tccgctccag gcgtccggcc aggtggcgct ccatggccgg
     1141 gaggacctcg gccaccggca cctggcggcc gagcaggctg gacagcgagc aggcggccac
     1201 ctcgggcatg ccgcagggca cgatcgcgtt gaagaaggcc aggtcggtgg cgcagttgag
     1261 cgcgaagccg tggctggtca ccccgcgcga gacccgcatg ccgatggcgc ccagcttggc
     1321 gtcgcccacc cagaccccgg tcaggtcgcc ccgctcgccc cgcgcctcca ggccgaagcc
     1381 ggcggccacg tcgaccaggg cctgctcgac ccgccccacg aaccgccaga cgtcggggct
     1441 ggggtcgagc agggtgatgg ggtagccgac gagctggccg gggccgtggt aggtgacctg
     1501 gccgccccgg tcgctgcgga ccacctcggc cccctgggcg gccagccagg ccgggtcggc
     1561 gagcacgtcg gcgtcgctgc cgcgtttgcc gatggtgtag acgtgcgggt gctccagcag
     1621 cagcacggtg tcggggatgt cgccggccgc ccggcgctcg accagggccc gctgccaggc
     1681 ccaggcccgc cggtagggga cggtcccggc ccgcacggtc cacagctcgc gcatggccta
     1741 ctcccgttcc aggcccaggt cgccttcgaa gtcggccgcc tccaggacct ccttgacctt
     1801 gaccaggaag cgggcggcgt cggcgccgtc gatgatgcgg tggtcgtagg tgaggatgcc
     1861 gtacatcatg tggcggaccg ccaccacctc gcccaggtcc gggtcctcga ccaccaccgg
     1921 ccgcttgacg atcgccgggg tggcgaagat ggccgcgttg ggctggttga tgatgggcgt
     1981 gtcccagacc gcgccccggc tgccggtgtt ggtcagcgag atggtggagc cggacagctc
     2041 gccggggtcg atcttgccgt cgcgggtgcg ggcggcgacg tcggcgatgg ccttggccag
     2101 gccggccagg ttcagcgact cggcgttctt gatcacgggg acgaacaggc ccttgggggt
     2161 gtccacggcc acccccaggt gcacgtcctg gtagacgtgg agattgccgt cctcgtccat
     2221 ggccgagttg accgtcgggt gctcccggat ggcggcgacc agggcgagga cggcgaaggg
     2281 catgggggag agggacacgc cctcgcgctg tttgaactcc tccttggccc gctcgcgcag
     2341 gcgcatgacc cgggtcatgt cgatctcgac ggcgctggtc agctgggcgc tggtctggag
     2401 cgaggcgacc atccgctcgg cgatcacctt gcgcagccgg gtcgcgggga cgacctcctc
     2461 gcgcaggccc ttggccaggg gcacggccac ctggggcgag gccgacgggg cggccgccgc
     2521 cgccttgccc ttgggctcgg ccctggccgc cgcggccggc tccttgccac gctgctggac
     2581 gaaggcctcg acgtcctcgc ggcgcacccg gcccccggac ccgctgccct ggacctggct
     2641 caggtcgacg tcgtgctcgc gggccagccg ccgcaccagc ggcgacaccc cggccgcggc
     2701 cttgccgttc cccttgccgt cgcctttcgc cttggcggcc ggcttgtcct ccttcggctt
     2761 gtcctccttc ggcttgggct ccttcggctt gggctcctcc ttggcgggct gctcctcggc
     2821 ctc
//
LOCUS       DASSWR010000332         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_408453,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000332 DASSWR010000000
VERSION     DASSWR010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408453"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VFO47_05550"
     CDS             <1..1638
                     /locus_tag="VFO47_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788193.1"
                     /translation="VATRPATGGRLTGTGRLVRLAIRRDRVVATVWVLGLFVFVLSQA
                     ASIVSLYPTQADLDRLARTATGIGANPAVVALQGPAYDASTYGGATAWQVVTPGVFLI
                     GLMSLLLMTRHTRQEEETGRAELVSAGVVGRYAWLAASLLFVVGVNVVLAVLSVLGYV
                     AVGLPVGGSVALAVGCGLNGLVFAGVAAVTAQLTEYARPANGMAFAALGAAFLLRAVG
                     DTASGLSWLAWLSPIGWAERFQPFAGERWAVLLLPVALLLVLLVAVVALRDRRDLGAG
                     VLATRLGPATAPAWLRSPLALAWRLQRGALLGWTVGAAVAGLSFGVLAQDMAQFAAED
                     PETAEMLASLGGAGSITDIYLAAVLSWIGLLAAGYAVQATLRLRSEEAELRAEPVLAT
                     TASRVGWAGSHLLVAAAGAAVVLAAGGLVAGLAHGLRAGDLGGQLPRVLGAALVQLPA
                     VWVLAALGAALFGLLPRLVVGATWAILGVVLAITMFGEALQLSRWLLDLSPFAHLPRL
                     PAATFTATPLLWLVIVAVLLAAAGLAAFRRRDLVSSA"
     gene            complement(1669..>2850)
                     /gene="pafA"
                     /locus_tag="VFO47_05555"
     CDS             complement(1669..>2850)
                     /gene="pafA"
                     /locus_tag="VFO47_05555"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HET8788194.1"
                     /translation="SHPEYATPECDSVTELVTHDKAGERILESLLAAAEARLHEEGIA
                     GKIILFKNNTDSAGNSYGCHENYLVSRYGEFQRLADVLIPFFVSRQILCGAGKVLATP
                     RGAVYCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEWS
                     AFLKVGATDLVLAMVEEATVLRDLTLENPIRAIREISHDMSCRRKVRLANGREASALE
                     IQREYFEKAARFVDRRDAGETAKRVVDMWGHVLEAIEHQELDRIGRQVDWVTKYLMIE
                     RARARHNLPLSHPKIALMDLTYHDVNRKRGLYYLLERNGSVERVCDDRAIDRATQEPP
                     QTTRARLRGEFIRQAKERRRDYTVDWVHLKLNDQAQRTVLCKDPFRAHDDRVDKLIAS
                     M"
BASE COUNT          283 a         1026 c         1080 g          461 t
ORIGIN      
        1 gtggccacca ggccggcgac aggcggtcgc ctcaccggca ccggccggct ggtgcgactg
       61 gccatccgcc gcgaccgggt cgtggccacc gtctgggttc tcgggctgtt cgtgttcgtc
      121 ctcagccagg cggccagcat cgtctcgctg tacccgaccc aggccgacct ggaccggctg
      181 gccaggacgg ccaccggcat cggggccaac ccggcggtgg tggccctcca gggcccggcc
      241 tacgacgcca gcacctacgg cggggccacc gcctggcagg tggtcacccc cggggtgttc
      301 ctcatcggcc tgatgagcct gctgctgatg acccgccaca cccgccagga ggaggagacc
      361 ggccgggccg agctggtcag cgcgggcgtg gtcggccgct acgcctggct ggccgccagc
      421 ctgctgttcg tggtcggggt caacgtggtc ctggccgtcc tcagcgtcct cgggtacgtg
      481 gccgtgggcc tgccggtggg cgggtcggtc gccctggccg tcggctgcgg tctcaacggc
      541 ctggtcttcg ccggcgtggc cgccgtcacc gcccagctga ccgagtacgc ccggccggcc
      601 aacggcatgg ccttcgccgc cctgggcgcg gccttcctgc tccgggccgt cggcgacacc
      661 gccagcggcc tgtcctggct ggcctggctg tcgccgatcg gctgggccga gcgcttccag
      721 ccgttcgccg gcgagcgctg ggcggtcctg ctcctgcccg tggccctgct gctggtcctg
      781 ctggtggccg tggtcgccct gcgcgaccgg cgcgacctgg gcgccggggt gctggccacc
      841 cggctggggc cggcgaccgc gccggcctgg ctgcgcagcc cgctcgccct ggcctggcgg
      901 ctgcagcgcg gcgccctgct cggctggacc gtgggggcgg ccgtggccgg cctgtcgttc
      961 ggggtgctgg cccaggacat ggcccagttc gccgccgagg acccggagac ggccgagatg
     1021 ctggcctcat tgggcggggc cggcagcatc accgacatct acctggccgc cgtcctgtcc
     1081 tggatcggcc tgctggccgc cggctacgcc gtccaggcca ccctgcggct gcgctccgag
     1141 gaggccgagc tgcgggccga gccggtgctg gccaccaccg cctcccgggt cggctgggcc
     1201 ggcagccacc tgctggtggc cgccgccggg gcggcggtcg tgctcgccgc cggcgggctg
     1261 gtggccggcc tggcccatgg gctgcgcgcc ggcgacctgg gcggccagct gccccgggtg
     1321 ctgggcgccg ccctggtcca gctgccggcg gtgtgggtcc tggccgcgct cggggcggcc
     1381 ctgttcgggc tgctgccgcg gctggtggtc ggggccacct gggcgatcct gggggtggtg
     1441 ctggccatca ccatgttcgg ggaggcgctc cagctcagcc gctggctgct cgacctgtcg
     1501 ccgttcgccc acctgccgcg gctgccggcg gccaccttca cggccacccc gctgctctgg
     1561 ctggtgatcg tggcggtgtt gctggccgcg gccggcctgg ccgccttccg ccgccgcgac
     1621 ctggtcagca gcgcctgagc cccgaccggc cggcggcggc cgggtcggct acatgctggc
     1681 aatcagcttg tcgacccggt cgtcgtgggc ccggaacggg tccttgcaga ggacggtgcg
     1741 ctgggcctgg tcgttgagct tcaggtggac ccagtcgacg gtgtagtcgc ggcggcgctc
     1801 cttggcctgg cggatgaact cgccccggag gcgggccctg gtcgtctggg gcggctcctg
     1861 ggtggcccgg tcgatggccc ggtcgtcgca cacccgctcc accgacccgt tgcgctccaa
     1921 caggtagtag aggccgcgct tgcggttgac gtcgtggtag gtcaggtcca tgagcgcgat
     1981 tttggggtgc gacaggggca ggttgtggcg cgcccgggcc cgctcgatca tcaggtactt
     2041 ggtcacccag tcgacctggc ggccgatccg gtccagctcc tggtgctcga tcgcctcgag
     2101 cacgtgcccc cacatgtcga ccacccgctt ggccgtctcg ccggcgtcgc ggcggtcgac
     2161 gaaccgggcc gccttctcga agtactcccg ctggatctcc agagcgctgg cctcccgccc
     2221 gttggccagg cggaccttgc gccggcagct catgtcgtgg gagatctcgc ggatggcccg
     2281 gatcgggttc tccagggtca ggtcgcgcag gacggtggcc tcctcgacca tggccaggac
     2341 caggtcggtg gccccgacct tgaggaacgc cgaccactcg gacatgttcg agtcgccaac
     2401 gatcacgtgc aggcgccggt agcgctcggc gtcggcgtgg ggctcgtcgc gggtgttgat
     2461 gatcggccgt gagcgggtgg tggccgagga gacgccctcc cagatgtgct cggcccgctg
     2521 ggcgatgcag tagaccgcgc ccctgggggt ggccagcacc ttgccggccc cgcagaggat
     2581 ctggcggctg acgaagaacg ggatcagcac gtcggccagc cgctggaact cgccgtagcg
     2641 cgacaccagg tagttctcgt ggcagccgta ggagttgccg gccgagtcgg tgttgttctt
     2701 gaacaggatg atcttgccgg cgatgccctc ctcgtggagg cgggcctcgg cggcggcgag
     2761 caggctctcc aggatccgct cccccgcctt gtcgtgggtg accagctcgg tcaccgagtc
     2821 gcactcgggg gtggcgtact cggggtggga
//
LOCUS       DASSWR010000333         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_578228,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000333 DASSWR010000000
VERSION     DASSWR010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578228"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFO47_05560"
     CDS             <1..540
                     /locus_tag="VFO47_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8788195.1"
                     /translation="DAVMMLTSLATRLGDRAGLVAFDREVRVVVGPGHARDQLSRVTE
                     AMYQLQPLLVESDYRGAFAETLARFRRRAMLVVLTELAEQAVSETLLPALPLVARDHL
                     VVVASVADPEVRGWALATPVEPGAAYRKAAAVAALADRRRTVARLRGLGAVVVDAPPG
                     RLAPELADAYLRVKATGRL"
     gene            595..1338
                     /locus_tag="VFO47_05565"
     CDS             595..1338
                     /locus_tag="VFO47_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET8788196.1"
                     /translation="MNENRIRRPAGVQAARAVLVTAALVAVLAAGCGVPRDPESTLDT
                     VRGGTLRAGITASDPWTTLEGGRPGGVEVELVERFARELGARVEWVDGSEADLIGALE
                     VRELDLVVGGLTADTPWQRKAAITRSYATTRVVVAVPASQPPPDDIAGVRVAVEAGTD
                     AAGILEEKTDAIPVRVPDVTQVKGSAVAIDEWLLADLDLRDTGVHLIKTKHVMATPLG
                     ENAFLVRLERFLIANQADVPALLDAETRR"
     gene            1358..>2044
                     /locus_tag="VFO47_05570"
     CDS             1358..>2044
                     /locus_tag="VFO47_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET8788197.1"
                     /translation="MSSHHELPPDKHEKLLKAVRLEWFSILYLLSAIVLLYFTLGNSQ
                     AMKAAWVEDILGLTPPIAFLVASRVRKRKPNDRFPYGYHRATAIAYLCASVALLALGA
                     FILYDSASKLIMFEHPPIDLVKPFGDRPIWLGWLMLGALAYSAVPQVFLGRLKQPLAK
                     ELNDKVLFADAEMNRADWMTAGAAMAGVIGIGFGLWWADSVAASIISVNIVHDGYKNL
                     KVAVGDLMDKE"
BASE COUNT          273 a          735 c          748 g          288 t
ORIGIN      
        1 gacgccgtca tgatgctgac ctcgctggcc acccggctcg gcgaccgggc cgggctggtc
       61 gccttcgacc gcgaggtccg ggtcgtggtc gggcccgggc atgcccgcga ccagctctcc
      121 cgggtcaccg aggccatgta ccagctccag ccgctgctgg tggagagcga ctaccggggc
      181 gccttcgccg agaccctggc ccggttccgc cgccgggcca tgctggtcgt cctgaccgag
      241 ctggccgagc aggccgtgtc cgagaccctc ctgccggcgc tgccgctggt tgcccgcgac
      301 cacctggtcg tggtggccag cgtggccgat ccggaggtgc gcggctgggc cctggccacg
      361 ccggtcgagc cgggcgccgc ctaccgcaag gccgccgccg tggccgccct ggccgaccgc
      421 cggcgcaccg tcgcccgcct gcgcggcctg ggcgcggtgg tggtcgacgc tccgcccgga
      481 cgcctcgccc ccgaactggc cgacgcctac ctgcgggtca aggccaccgg gcggctctga
      541 acggcgggag gtttgggaac cagcaaggcg agtaatagtt ttcgaggtgc gccgatgaac
      601 gagaaccgga tcaggcgacc agccggggtc caggctgccc gggcggtcct ggtgacggcc
      661 gcgctcgtgg ccgtgctggc cgccgggtgc ggggtcccgc gggacccgga gagcaccctg
      721 gacacggtcc ggggcgggac cctgcgggcc gggatcacgg ccagcgatcc ctggacgacc
      781 ctcgaagggg gccggccggg cggggtcgag gtcgagctgg tcgagcggtt cgcccgcgag
      841 ctgggggccc gggtcgagtg ggtcgacggg tcggaggccg acctgatcgg cgccctcgag
      901 gtgcgggagc tggacctggt cgttggcggc ctcaccgccg acacgccctg gcagcgcaag
      961 gccgccatca cccgctcgta tgccaccacc cgggtggtgg tcgcggtccc ggcgtcgcag
     1021 ccgccgcccg acgacatcgc cggggtccgg gtcgcggtcg aggccggcac cgacgcggcc
     1081 gggatcctgg aggagaagac cgacgccatc ccggtccggg tcccggacgt gacccaggtc
     1141 aaggggtcgg ccgtggccat cgacgagtgg ctgctggccg acctcgacct ccgcgacacc
     1201 ggcgtccacc tgatcaagac caagcacgtc atggccaccc cgctgggtga gaacgccttc
     1261 ctggtccggc tggagcggtt cctgatcgcc aaccaggcgg acgtgcccgc cctgctggac
     1321 gcggagaccc gccggtgaac gcggccgggg tcggcccatg agcagccacc acgagctgcc
     1381 cccggacaag cacgagaagc tgctcaaggc cgtgcggctg gagtggttct cgatcctgta
     1441 cctgctgtcg gcgatcgtcc tgctctactt cacgctcggc aactcccagg cgatgaaggc
     1501 cgcctgggtc gaggacatcc tcgggctcac cccgccgatc gcgttcctgg tcgcctcgcg
     1561 ggtgcgcaag cgcaagccga acgaccggtt cccctacggc taccaccggg ccacggccat
     1621 cgcctacctg tgcgcctcgg tggccctgct cgccctgggc gcgttcatcc tgtacgactc
     1681 ggcctccaag ctgatcatgt tcgagcaccc gccgatcgac ctggtcaagc cgttcggcga
     1741 ccggccgatc tggctgggct ggctgatgct gggggcgctg gcctacagcg ccgtgcccca
     1801 ggtgttcctg ggccggctca agcagccgct ggccaaggag ctcaacgaca aggtgctgtt
     1861 cgccgacgcc gagatgaacc gggccgactg gatgacggcg ggcgcggcca tggccggggt
     1921 gatcggcatc gggttcggcc tgtggtgggc cgactcggtg gcggccagca tcatctcggt
     1981 caacatcgtc cacgacggct acaagaacct gaaggtcgcc gtcggcgacc tgatggacaa
     2041 ggag
//
LOCUS       DASSWR010000334         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1142426, whole genome shotgun sequence.
ACCESSION   DASSWR010000334 DASSWR010000000
VERSION     DASSWR010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142426"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="prcB"
                     /locus_tag="VFO47_05575"
     CDS             <1..525
                     /gene="prcB"
                     /locus_tag="VFO47_05575"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HET8788198.1"
                     /translation="AAGPAIEMVRLFQTELEHYEKIEGFPLSLEGKANKLGQMVRGNL
                     PLAMQGLVVLPLFCGYDTRRQAGRIFRYDVTGGRWEDADYHATGSGGKDARGSLKKRW
                     RAGMDGDSAVAVLVEALYDAADEDAATGGPDLVRGIFPVVATVTAEGYRRVPDDDLRK
                     AAERLVAARAQGEG"
     gene            528..1223
                     /gene="prcA"
                     /locus_tag="VFO47_05580"
     CDS             528..1223
                     /gene="prcA"
                     /locus_tag="VFO47_05580"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET8788199.1"
                     /translation="MSVPFYVPPEQLTKEKAEFARKGIGRGRPLIVLEYAGGILFVAE
                     NPSTHLHKVAELYDRIAFAAVGRFSEFENLRQAGVRLADIRGYAYGREDVTSRSIANA
                     YSQTLGQIFVQEFKPYEVELCVAEVGVDGAPNAMYHIVFDGNVFDRKGFLAMGGQSDE
                     LNSELQRGYDEDMELDGALQLAMRVLRQAAPAGETGNSRLEVAVLERDRPRRSFRRLT
                     QEDVSSRAGEPAS"
     gene            1220..>2027
                     /locus_tag="VFO47_05585"
     CDS             1220..>2027
                     /locus_tag="VFO47_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788200.1"
                     /translation="MSPPPPGGPGWPPPAPDAERQDRPSWLPQPAPPPEPPPGRRVRG
                     LTAALILLLVVALAATGLVFATRRDPPPEPGAAGGTAGATPTTGAPAGATPTTRAPAP
                     AVAAPAVRAVQRDVSDLRDLRFERRVPVTVESPDKLAKRLLRTLAEETDEDQLRRQGR
                     AMELLGELPPGTDLPRLLNRLQAESVLGFYLPGRRPPKGGLYVRSSRGLDPYAKIILA
                     HELTHAVTDQRFDLTRADRLAVATARQDELAAYSSLVEGDATLTMERYLTE"
BASE COUNT          274 a          785 c          728 g          240 t
ORIGIN      
        1 gcggccgggc cggcgatcga gatggtgcgg ttgttccaga ccgagctgga gcactacgag
       61 aagatcgagg gcttcccgct gtcgctggag ggcaaggcga acaagctcgg ccagatggtc
      121 aggggcaacc tccccctggc catgcagggc ctggtcgtgc tgcccctgtt ctgcggctac
      181 gacacccgcc ggcaggccgg ccgcatcttc cgctacgacg tcaccggcgg ccgctgggag
      241 gacgccgact accacgccac cggctcgggc ggcaaggacg cccgcggcag cctcaagaag
      301 cgctggcggg ccggcatgga cggcgacagc gccgtcgccg tgctggtcga ggcgctgtac
      361 gacgccgccg acgaggacgc cgccaccggc gggcccgacc tggtccgggg gatcttcccg
      421 gtggtggcca cggtcaccgc cgagggctac cggcgggttc ccgacgacga cctgcgcaag
      481 gcggccgagc gcctggtcgc cgcccgcgcc caaggagagg gctgacggtg agcgttccct
      541 tctacgtccc gcccgagcag ctcaccaagg agaaggccga gttcgcccgc aagggcatcg
      601 gccggggccg gccgctgatc gtgctcgagt acgccggcgg gatcctgttc gtggccgaga
      661 acccctcgac ccacctgcac aaggtggccg agctgtacga ccggatcgcg ttcgccgccg
      721 ttgggcggtt ctcggagttc gagaacctgc gccaggccgg ggtgcgcctg gccgacatcc
      781 gcggctacgc ctacggccgc gaggacgtca cctcgcgctc gatcgccaac gcctactccc
      841 agaccctcgg gcagatcttc gtccaggagt tcaagcccta cgaggtcgag ctgtgcgtgg
      901 ccgaggtcgg cgtcgacggc gcccccaacg cgatgtacca catcgtgttc gacggcaacg
      961 tgttcgaccg caagggcttc ctggccatgg gcggccagag cgacgagctc aacagcgagc
     1021 tgcagcgcgg ctacgacgag gacatggagc tggacggggc cctgcagctg gccatgcggg
     1081 tcctgcgcca ggccgccccg gccggcgaga cgggcaactc ccgcctcgag gtggccgtgc
     1141 tggagcgcga ccggccccgg cgctcgttcc ggcgcctgac ccaggaggac gtctcctccc
     1201 gtgccggtga accggcgtcg tgagcccgcc cccgcccggc ggcccgggct ggccaccccc
     1261 cgctccggac gccgagcggc aggaccggcc gtcctggctg ccgcagccgg cgccgccgcc
     1321 tgagccgccg cccgggcggc gggtccgggg gctgacggcg gcgctgatcc tgctgctggt
     1381 ggtcgccctg gccgccaccg ggctggtgtt cgccacccgc cgggacccgc cgccggagcc
     1441 cggcgcggcc ggcgggacag cgggcgccac gccgacgacc ggggcgccgg ccggcgccac
     1501 ccccacgacc cgggcgccgg cgcccgccgt ggccgccccg gcggtccggg ccgtgcagcg
     1561 cgacgtgagc gacctgcgcg acctccgctt cgagcggcgg gtcccggtga ccgtcgagtc
     1621 gcccgacaag ctggccaagc ggctgctgcg gaccctggcc gaggagaccg acgaggacca
     1681 gctgcgccgc cagggccggg ccatggagct gctgggggag ctgcccccag gcaccgacct
     1741 gccccgcctc ctcaaccggc tccaggccga gagcgtgctc ggcttctacc tgcccggccg
     1801 ccgcccgccc aagggcggcc tgtacgtccg ctccagccgc ggcctcgacc cctacgccaa
     1861 gatcatcctg gcccacgagc tgacccacgc cgtcaccgac cagcgcttcg acctgacccg
     1921 ggccgaccgg ttggccgtgg ccaccgcccg ccaggacgag ctggccgcct actccagcct
     1981 ggtcgagggc gacgccaccc tgaccatgga gcgctacctg accgaga
//
LOCUS       DASSWR010000335         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_691212,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000335 DASSWR010000000
VERSION     DASSWR010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691212"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFO47_05590"
     CDS             <1..322
                     /locus_tag="VFO47_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8788201.1"
                     /translation="GGPKTWTAVLDVADLEEGRPRVVRAGDTDVLLYLEGNRLRALWA
                     RCTHEGGPLAEGTFADGCVTCPWHGSTFRLADGKVVRGPAAASQPVYEARVTDGKVEV
                     RTLP"
     gene            complement(324..914)
                     /locus_tag="VFO47_05595"
     CDS             complement(324..914)
                     /locus_tag="VFO47_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8788202.1"
                     /translation="MPIDRKSVTRELRLLLQGVDGLAGEGLAAALQRVADAAVMVLRA
                     QGAGVMLADEHDVLRCAAASGPPGRGLATAQERLGAGPALDSFAGDLPVTSRDVTADG
                     RWPDLRHLIDRAAVRAVLAAPLTLPGGRPIGALSLHSSRARDWEVGEIAATMAYGGVV
                     ASLISMALEARLRGVLLDRLLDALRGVADRPDAEGG"
     gene            complement(1024..3048)
                     /locus_tag="VFO47_05600"
     CDS             complement(1024..3048)
                     /locus_tag="VFO47_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET8788203.1"
                     /translation="MSEQPAPNRLAGQTSPYLLQHAHNPVDWYPWGPEALERARAEDK
                     PILLSIGYAACHWCHVMAHESFEDDETATQMNRDFVCVKVDREERPDLDGIYMDAVQA
                     MTGHGGWPMTVFLTPEGGPFYAGTYYPKVDRPGMPGFRRVLAAVADAWEKRRDDARRQ
                     GDQVVQVLAAQAGALGGDGDGQLAPDTLHQAFEGLRGAFDGTWGGFGAAPKFPQPMTL
                     EFLLRCHLRGEDGALGMVRLTLDRMAAGGIFDQVGGGFHRYSTDGRWLVPHFEKMLYD
                     NALLVRLYTHAWQLTGDDRYRAVAARTADYLLRELRHGDGGFFSSQDADSEGVEGRFF
                     VWSWDELAGIAGEPVARHLGASPEGNWEGTNVLWTPQPAEAGLEAEVAAALPRLFEAR
                     EARVHPATDDKVLAAWNGLAVSALAEAGRAFEEPRYTEAALAAAGFVLAELAGEDGRL
                     LRSWRDGGKGGPGYLDDYACMAEACLTLYESTFDLRWLRESRRLAAAMVELFADPGGD
                     GFYQTGRDAERLVVRPRELFDNAVPAGSSVAAEVLQRLGRLTGDPDAERAGLDALRPV
                     LGVLGRAPTGFGHALGAADFALARVREVAIVGRPGADDTRALLARVWGAYQPNRVLAA
                     AAPDDTTAQAEVPLLADRPALEGRATAYVCEHFVCQRPVTSPADLAAQLA"
     gene            3098..>3416
                     /locus_tag="VFO47_05605"
     CDS             3098..>3416
                     /locus_tag="VFO47_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8788204.1"
                     /translation="MDTEAVAAWLDGYSHAWGTYDPAEIGALFSEDAVYFYDPFTQPL
                     RGLEAIVADWLADRDEPGTYEGSYRPVLVAGDEAVARGYSRYYNTNGTIRDEYDNLFL
                     LRFD"
BASE COUNT          440 a         1264 c         1302 g          410 t
ORIGIN      
        1 cggcgggccc aagacctgga cggcggtgct ggacgtggcc gacctggagg agggccggcc
       61 ccgggtggtc cgcgccggcg acaccgacgt gctgctgtac ctcgagggca accgcctgcg
      121 ggccctgtgg gccaggtgca cccacgaggg cggtccgctg gctgaaggca ccttcgccga
      181 cgggtgcgtg acctgcccct ggcacggcag cacgttccgc ctggccgacg gcaaggtggt
      241 ccgcggcccg gcggccgcct cccagcccgt ctacgaggcc cgggtcaccg acggcaaggt
      301 ggaggtccgc accctcccct gagtcagccg ccctcggcgt cgggacggtc ggcgaccccg
      361 cgcagggcgt cgagcaggcg gtccagcagc accccgcgga ggcgggcctc cagggccatg
      421 gagatgaggc tggccaccac cccgccatag gccatggtgg cggcgatctc gcccacctcc
      481 cagtcccggg cccgggagct gtgcaggctg agggcgccga tcggccggcc cccgggcagg
      541 gtcagggggg cggccagcac ggcccggacg gcggcccggt cgatcaggtg gcgcaggtcg
      601 ggccagcgcc cgtcggcggt gacgtcacgg gaggtgaccg gcaggtcgcc ggcgaacgag
      661 tccagcgccg gtccggcgcc cagccgctcc tgggcggtgg ccaggccccg tcccggcggc
      721 ccgctggcgg cggcgcaacg cagcacgtcg tgttcgtcgg ccagcatcac cccggcgccc
      781 tgggcccgca gcaccatgac ggccgcgtcg gccacccgct ggagggcggc ggccaggccc
      841 tccccggcca gcccgtccac gccttggagc aggagccgca gctcccgcgt cacggacttc
      901 cggtcgatcg gcacgggcga cgcccctcgc gcgtccccgg ccaggagacg cgagctcgat
      961 tggccctgcc gcctgtgact tacctgcccg gggacctgcc aaaccccggc gttggcggcc
     1021 ggttcaggcc agctgggcgg ccaggtcggc cggggaggtc acgggccgct ggcagacgaa
     1081 gtgctcgcaa acgtacgcgg tggcgcggcc ctccagggcc ggccggtcgg ccagcagcgg
     1141 cacctcggcc tgggcggtcg tgtcgtcggg ggcggccgcc gccagcaccc ggttgggctg
     1201 gtaggcgccc cacacccggg cgagcagggc ccgggtgtcg tcggccccgg ggcggcccac
     1261 gatcgccacc tccctgaccc tggccagggc gaagtcggcc gccccgaggg cgtgcccgaa
     1321 cccggtcggg gcccggccga gcaccccgag gacgggccgg agcgcgtcca gcccggcccg
     1381 ctcggcgtcc gggtcgccgg tcaggcggcc gaggcgctgg agcacctcgg cggccaccga
     1441 cgagccggcc gggacggcgt tgtcgaacag ctcccggggc cggaccacca gccgctcggc
     1501 gtcgcggccg gtctggtaga agccgtcgcc gccggggtcg gcgaacagct cgaccatggc
     1561 cgcggccagc cgccgggatt cgcgcagcca gcgcaggtcg aaggtggact cgtacagggt
     1621 caggcacgcc tcggccatgc aggcgtagtc gtccaggtag ccgggcccgc ccttgccgcc
     1681 gtcgcgccag gagcgcagca gccgcccgtc ctcgccggcc agctcggcga gcacgaaccc
     1741 ggccgcggcc agggcggcct cggtgtagcg gggctcttcg aaggcccgcc cggcctcggc
     1801 cagggccgag acggccaggc cgttccaggc ggccagcacc ttgtcgtcgg tcgccggatg
     1861 gacccgggcc tcgcgggcct cgaacaaccg cggcagcgcg gccgcgacct cggcctccag
     1921 ccccgcctcg gcgggctggg gggtccagag aacgttggtg ccctcccagt tgccctcggg
     1981 gctggctccc aggtgccggg ccaccggctc gccggcgatc ccggccagct cgtcccatga
     2041 ccagacgaag aagcggccct cgaccccctc ggagtcggcg tcctgggagg agaagaaccc
     2101 gccgtcgccg tggcgcagct cgcgcagcag gtagtcggcc gtgcgggcgg ccaccgcccg
     2161 gtagcggtcg tcgccggtga gctgccaggc gtgggtgtag agccgcacca gcagggcgtt
     2221 gtcgtacagc atcttctcga agtgcgggac cagccagcgc ccgtcggtcg agtagcggtg
     2281 gaagccgccg ccgacctggt cgaagatgcc gccggcggcc atccggtcaa gggtcagccg
     2341 gaccatgccg agcgccccgt cttcgccgcg caggtggcag cggagcagga actcgagcgt
     2401 catcggctga gggaacttgg gggcggcccc gaacccgccc caggtgccgt cgaacgcccc
     2461 ccggaggccc tcgaacgcct ggtgcagcgt gtcgggggcg agctggccgt cgccgtcccc
     2521 gccgagggcg ccggcctggg cggccagcac ctggacgacc tggtcgccct ggcgtcgggc
     2581 gtcgtcgcgg cgcttctccc aggcgtcggc gacggcggcc agtacccggc ggaagccggg
     2641 catgcccggc cggtcgacct tcgggtagta ggtgccggcg tagaaggggc cgccctcggg
     2701 ggtcaggaac accgtcatcg gccagccgcc gtggccggtc atggcctgga ccgcgtccat
     2761 gtagatgccg tccaggtcgg gccgctcctc acggtcgacc ttgacgcaga cgaagtcccg
     2821 gttcatctgg gtggccgtct cgtcgtcctc gaacgactca tgggccatca cgtggcacca
     2881 gtggcaggcg gcgtagccga tggagagcag gatcggcttg tcctcggccc gggcccgctc
     2941 cagggcctcg ggcccccacg ggtaccagtc gaccgggttg tgggcgtgct ggaggagata
     3001 ggggctggtc tggccagcca gtcggttggg ggcgggctgc tcgctcatcg gctcagtgta
     3061 ggctcggacg gcgaaccggc tgagcaagga ggacgccgtg gacaccgagg cggtggcggc
     3121 ctggctggac ggctacagcc atgcctgggg cacctacgac cccgcggaga tcggggcgct
     3181 gttcagcgag gacgccgtct acttctacga cccgttcacc cagccgctgc gggggctgga
     3241 ggcgatcgtg gccgactggc tggccgaccg cgacgagccc ggcacctacg agggcagcta
     3301 ccggccggtc cttgtcgccg gggacgaggc ggtggccagg ggctacagcc gttactacaa
     3361 caccaacggc accatccgcg acgagtacga caacctcttc ctgctccgct tcgacg
//
LOCUS       DASSWR010000336         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_126074,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000336 DASSWR010000000
VERSION     DASSWR010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126074"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1018
                     /locus_tag="VFO47_05610"
     CDS             188..1018
                     /locus_tag="VFO47_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HET8788205.1"
                     /translation="MSSAVVDFGVDPIDPPDPAASPETLEVRPEDEVAVQRVEQWFRD
                     YAQSRDPALRERIILSYLGLADRLAERYRSNRAVPLEDLRQVARLGLVKAVDRYQPER
                     ANPFIPYAVATVVGELKRHLRDASWRLRVPRGTKDLALRLCRAIDELPQQLGHSPTVP
                     ELAEHLGASMEEVLEAIEVAQTRSAPSLDQPAGEDGDAVLGDFVADRRHREELEDLLV
                     LPELIARLPERERQIVLLRYVDELTQDEIAARMQISQMHVSRLLRRAIERMRCQLVDL
                     "
     gene            complement(1145..>2090)
                     /locus_tag="VFO47_05615"
     CDS             complement(1145..>2090)
                     /locus_tag="VFO47_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788206.1"
                     /translation="WSAPGPSTCASTSGSPPRTWPSATGFGLLLGALGIGSVAGAVAL
                     PKLRQAVPIDRRVVAGTVLFALATAALALLDSPALVFVAMAAAGLAWLAILTSFNVAT
                     QTAVPRWVRARALAVYLLVFQGGLAAGSALWGVAAGRLGERTALLAAAASFGLGMVAA
                     LRWRLQGIGALDLTPSVRPEPVTVVDPDPEDGPVLVLIRYRIDPGRAEEFATAMRAMR
                     RVRRRDGAYRWGVFEDVADPGCFVETYVVRSWAEHLRQHERFTAEDLAVRDRVRLFHV
                     GEDPPVVSHFIHPDAAPTQRRWVRWTRRHGDLAQAGEE"
BASE COUNT          286 a          790 c          763 g          251 t
ORIGIN      
        1 cggcccggtg gtggccgtgc gcccgcgccg gtgggcgtgg tggtcggccg cctgatctgg
       61 tcgaaatgta cgaacctacc cggagatcgg gtttatctcc gtgtggggag ggacatgcta
      121 gggttggcag gcacttgaca ggtcccgccc tgtccccgcg ggccgaccgc acctcgtgga
      181 ggtcgctatg tcgtccgcgg ttgtcgactt tggcgtcgac ccgatcgatc ctccggaccc
      241 tgcagcgtca cccgagacgc tcgaggtccg cccggaggac gaggtcgccg tccagcgcgt
      301 cgagcaatgg ttccgcgact acgcccagag ccgtgacccg gccctgcgcg agcgcatcat
      361 cctctcctac ctcggcctgg ccgaccggct ggccgagcgc taccggagca accgggccgt
      421 ccccctggag gacctgcgcc aggtggcccg gctcgggctg gtcaaggcgg tcgaccgcta
      481 ccagccggag cgcgccaacc cgttcatccc ctacgccgtg gccaccgtgg tcggcgagct
      541 caagcggcac ctgcgcgacg ccagctggcg gctgcgggtg ccccgcggca ccaaggacct
      601 ggccctgcgg ctctgccggg ccatcgacga gctgccccag cagctcggcc actcccccac
      661 cgtgcccgag ctggccgagc acctcggggc cagcatggag gaggtcctgg aggcgatcga
      721 ggtcgcccag acccgctcgg cgccgtcgct cgaccagccg gccggggagg acggcgacgc
      781 ggtgctgggc gacttcgtgg ccgaccgccg ccaccgcgag gagctggagg acctgctggt
      841 cctgcccgag ctgatcgcgc ggctgccgga gcgcgagcgc cagatcgtcc tgctgcgcta
      901 cgtcgacgag ctgacccagg acgagatcgc cgcccgcatg cagatctccc agatgcacgt
      961 ctcgcgcttg ctgcgccggg cgatcgagcg catgcgctgc cagctcgtcg acctctaggg
     1021 ccggccgggc cgtcggccgc tgcgggtttg gaagcgttcc ggctggcaac gcctcttccc
     1081 gggagggccg ggaccccgcc ctcctaggcc tcaagggtgc cggtgtcagg gcacccttga
     1141 ggcgctactc ctcgcccgcc tgggccaggt cgccgtggcg gcgggtccag cgcacccagc
     1201 ggcgctgggt gggggcggcg tcggggtgga tgaaatggga gaccaccggc gggtcctcgc
     1261 cgacgtggaa caaccggacc cggtcgcgga cggccaggtc ctcggcggtg aaccgctcgt
     1321 gctggcgcag gtgctcggcc caggagcgga ccacgtaggt ctcgacgaag cagcccgggt
     1381 cggccacgtc ctcgaacaca ccccaacggt aggcgccgtc gcgccggcgg acccggcgca
     1441 tggcccgcat cgcggtcgcg aactcctcgg cccggcccgg gtcgatccgg tagcggatca
     1501 gcaccagcac gggaccgtcc tcggggtcgg ggtcgaccac cgtcaccggc tccgggcgga
     1561 ccgacggggt caggtcgagg gcgccgatcc cctggaggcg ccagcgcagc gcggccacca
     1621 tccccaggcc gaagctggcc gcggccgcca gcagggccgt ccgctcgccc aggcgacccg
     1681 cggccacccc ccagagggcg ctgccggcgg ccaggccgcc ctggaacacc agcaggtaca
     1741 cggccagggc ccgggcccgg acccaccggg gcacggccgt ctgggtggcc acgttgaacg
     1801 aggtcaggat ggccagccag gccagcccgg cggcggccat ggccacgaac accagagccg
     1861 gcgagtccag cagggccagg gccgccgtcg ccagggcgaa caggacggtg ccggccacca
     1921 cccgccggtc gatgggcacg gcctggcgca gcttcggcag ggccaccgcc ccggccaccg
     1981 acccgatccc gagcgcgccc agcagcagcc cgaacccggt cgcggacggc caggtcctcg
     2041 gcggtgaacc gctcgtgctg gcgcaggtgc tcggcccagg agcggaccac
//
LOCUS       DASSWR010000337         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1142590, whole genome shotgun sequence.
ACCESSION   DASSWR010000337 DASSWR010000000
VERSION     DASSWR010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142590"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..>2504
                     /locus_tag="VFO47_05620"
     CDS             298..>2504
                     /locus_tag="VFO47_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8788207.1"
                     /translation="MDVTWPLVGRQAELEVLSAVLADTRAGGVVLVGEAGIGKTRMAR
                     EALARAEAAGWGVEWLAATRAAASIPFGAVSHLLPPAERLGDDRLDTLRRTAELLAER
                     SRGRPLLLGVDDAHLLDDASAALVHQLAFRGIAVVVATMRTGEPAPDPVIALWKDGLT
                     RRLDLSALPPSATAELLERTLGGPVDGVTREEVLRITGGNPLYLRELVLGGLESGALR
                     QVDKVWRWRGKLAHATRLAELVQARLSTLDETARVAVELVAWGEPLGVGVLERLVGKD
                     AVQAAEDGGLLVLERSGRRTLARLAHPLYGEVLRAVQPLSRARAVAERLAADFGAGAL
                     RRRDDLLRVGAWQLEAGMATNPDLLLEAARQAAGRFDHELTERLARAAVDAGGGPTAV
                     RVLAETLEWQGRHAEAVAVMDGEPPAQGAERARWASIRAGNLYWGLERTAEAEKVLQQ
                     AAVGEEGGEEAVAMLAWILLFDGRLPEAVAVAGRVLDRPEVPPQALVWAATAAVPALG
                     SLGRLGEALAVADRGLAVAELHPQDLPWGETQLNLVRCQVLLGAGRLTEASAIADAGY
                     QAAVADRSSERTGGWAGFRGLVAKAEGRVATAEAWLREAVALLDEQDPYRFMRWCLAE
                     LASVAALAGDQEAAVGWLERADARAGEANRYFDPWVELDKAWVAAGAGELTRAVALAT
                     RAADMARASKQFTLEAAALYDVARLGAPAAVRQRLEELVGLLEGRLAALLATSA"
BASE COUNT          295 a          868 c          997 g          344 t
ORIGIN      
        1 gccccagggc atcgttcccg tcgagttcac gacctcggca tcggcactgt cggcagaggg
       61 gcatggggcc gcgggccggc agggtcggaa ccgggcggcg accccccgaa gccgcccggc
      121 gccgcctccc ccccggaccg accacggctc gcggcgtgag gaacccaccg ccgagcatgt
      181 cgggaaaccg gagccgccgc tcccggttgc gccgaccgga cgctattccc aggcggctgc
      241 cctgtcttga gtagttcact actctattgg ccggtccgat cggttgagga ggcctgggtg
      301 gacgtcacgt ggccgctggt ggggcgccag gccgagctcg aggtgctgtc cgccgtcctt
      361 gcggacaccc gtgccggcgg tgtggtgctc gtcggtgagg ccgggatcgg caagacgagg
      421 atggcccggg aagccctggc ccgggccgag gcggcggggt ggggggtcga gtggctggcg
      481 gcaacccggg cggccgcttc gatcccgttc ggggccgtct cccacctgct gcccccggcc
      541 gagcgcctcg gcgacgatcg cctcgacacc ctccgccgga ccgccgagct gctcgccgag
      601 cggtctcgtg gccggccgct gctgctcggg gtcgacgacg ctcacctgct cgacgacgcc
      661 tcggccgcac tggtgcacca gctcgcgttc cggggcatcg ccgtggtggt cgccaccatg
      721 cggaccggcg agccagcccc cgacccggtg atcgcgctct ggaaggatgg cctgacccgc
      781 cgcctggacc tgtcggcgct gccgccgtcg gcaaccgccg agctgctcga gcggactctg
      841 ggcgggccgg tcgacggcgt gaccagggag gaagtgctgc ggatcaccgg cggcaaccca
      901 ctgtacctgc gcgagctcgt cctgggcggg ctggagagcg gcgccctgcg ccaggtggac
      961 aaggtgtggc gttggagggg caagctcgcg cacgcgacgc gcctggccga gctggtccag
     1021 gcccgccttt ccacgctcga cgagacggcc agggttgcag tcgaactggt cgcttggggt
     1081 gagcccctgg gcgtcggtgt cctcgaacgg ctggttggca aggacgcggt ccaggcagcc
     1141 gaggacggcg gcctgctggt gctggagcga tcgggccgcc gcacgctcgc ccggctcgcc
     1201 caccccctgt atggcgaggt gctgcgggct gtccaaccgc tgagccgagc gcgggcggta
     1261 gccgagcggc tggcggccga tttcggcgct ggcgccctac ggcgacggga cgacctgttg
     1321 cgggtaggcg cctggcagtt ggaagccggc atggccacca acccagacct gctgctggag
     1381 gcggccaggc aggccgctgg ccgcttcgac catgagctga ccgaacgcct ggcccgtgcc
     1441 gcggtcgatg ccgggggtgg cccgaccgcg gtgcgggtcc tggccgagac cctcgagtgg
     1501 cagggccgac acgccgaggc ggtggcggtg atggacgggg agccgccggc ccagggcgcc
     1561 gagcgggccc gctgggcaag cattcgggcc gggaacctgt actgggggct ggagcggacc
     1621 gctgaggccg agaaggtctt gcagcaggcg gcggtgggcg aggagggtgg cgaggaagcg
     1681 gtcgccatgc tggcctggat cctgctgttt gacggccgcc ttcccgaggc cgtagccgtg
     1741 gccggccggg tcctggatcg gccggaggtt cccccgcagg cgctggtgtg ggccgcgacc
     1801 gcggccgtgc ccgccctggg gtcgcttggt cggctcggcg aggcgctggc cgtggccgac
     1861 cgcgggctgg ccgtggccga gctccacccg caggacctgc cctggggcga gacccagctc
     1921 aacctggtcc gctgccaggt gctgctcggc gccgggcggc tcaccgaggc cagcgcgatc
     1981 gccgacgccg ggtaccaggc tgcggtcgcc gaccgctcat cggagcggac cgggggttgg
     2041 gccggcttcc ggggcctggt cgccaaggcc gagggccggg tcgcgaccgc cgaggcgtgg
     2101 ctgcgggagg cggtcgccct gctggatgag caggatccct atcggttcat gcgctggtgt
     2161 ctggccgaac tggccagcgt ggccgccttg gccggcgatc aggaggcggc ggtgggctgg
     2221 ctggagcgcg cggacgcccg cgccggagag gccaaccgct actttgatcc ctgggtggag
     2281 ctggacaagg catgggtggc cgccggtgcg ggtgagctca ccagagccgt cgccctcgcg
     2341 acccgggcgg cagacatggc ccgggcttcc aagcagttca ccctcgaggc ggccgccctg
     2401 tacgacgttg cccgcctggg cgctcccgcc gccgtccggc agcggctgga ggaactggtt
     2461 ggcctgctgg agggacggct ggcggcgctg ctggccacct cggc
//
LOCUS       DASSWR010000338         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_578386,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000338 DASSWR010000000
VERSION     DASSWR010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578386"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /gene="aceE"
                     /locus_tag="VFO47_05625"
     CDS             complement(<1..1256)
                     /gene="aceE"
                     /locus_tag="VFO47_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949010.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET8788208.1"
                     /translation="MAMLNDVDPVETREWLEALDSVLAFEGTDRARFLLGELSAEGRR
                     RGAPAPYSATTPYLNTIPVDQQPAYPGDLATEHKIRSLNRWNALAMVLRANKESSELG
                     GHIASFQSSATLYDTGYNHFWHAPSEGHGGDLVYFQGHSSPGFYARSFLEGRFTEAQM
                     DRFRQEVDGGGLPSYPHPWLMPDYWQFPTVSMGLGPIMAIYQARFLKYLGTRGLADTA
                     GRKVWAFLGDGETDEPESLGAIPLAGRERLDNLIFVVNCNLQRLDGPVRGNGKIIQEL
                     EGIFRGAGWNVIKVIWGSGWDPLIANDKSGILLKRMEEAVDGEYQDFKSKNGAYVREH
                     FFGKYPELLELVAGMTDDDIWALTRAGHDPSKVYAAYSAAVRTVGRPTVILAKTVKGY
                     GMGEAGEGQNITHQQKKVGGAALARFR"
     gene            1460..>2054
                     /locus_tag="VFO47_05630"
     CDS             1460..>2054
                     /locus_tag="VFO47_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788209.1"
                     /translation="MGILVAFAVIAAAVAVAVTAMLLVRRRAPEGSFFNDGDRASGVF
                     GVLATGFAVLLGFIVVLAFTSYDESKSGAELEALAVVQQFETAQFLPAAAGQRVAGEL
                     ICYGRSVVGQEWPRMQTGAETGALNPWSVAMFRTMKTIEPATAAEQSAYDRWLDQTST
                     REEARNDRVHAAEGVIPTPLWRVLFFSGGLIVLDMLFF"
BASE COUNT          295 a          730 c          688 g          341 t
ORIGIN      
        1 cggaaccggg ccagtgccgc accgccgacc ttcttctgct ggtgggtgat gttctgaccc
       61 tcgcccgcct cgcccatccc gtagcccttg accgtcttgg ccaggatcac ggtcggtcgc
      121 cccacggtcc gcaccgccgc gctgtaggcg gcgtagacct tgctggggtc gtgcccggcg
      181 cgggtgagcg cccagatgtc gtcgtcggtc atgccggcga cgagctcgag cagttcgggg
      241 tacttgccga agaagtgttc ccggacgtag gcgccgttct tggacttgaa gtcctggtac
      301 tcgccgtcga ccgcctcctc catgcgcttg aggaggatgc cgctcttgtc gttggcgatc
      361 agcgggtccc agccggagcc ccagatgacc ttaataacgt tccacccggc gccgcggaag
      421 atgccctcca gctcctggat gatcttgccg ttgccccgga ccgggccgtc caggcgctgc
      481 aggttgcagt tgacgacaaa gatgaggttg tccagccgct cccggccggc gagcgggatc
      541 gccccgagcg actccggctc gtcggtctcg ccgtcgccca ggaacgccca caccttgcgg
      601 ccggcggtgt cggccagccc gcgggtgccc aggtacttca ggaaccgggc ctggtagatg
      661 gccatgatcg gccccagccc catcgacacc gtcgggaact gccagtagtc cggcatcagc
      721 cacgggtgcg ggtaggacgg cagcccgccg ccgtcgacct cctgacggaa ccggtccatc
      781 tgggcctcgg tgaacctacc ctccaggaac gaccgggcgt agaagccagg cgaggagtgg
      841 ccctggaagt agaccaggtc gccgccgtgg ccctccgagg gggcgtgcca gaagtggttg
      901 tagccggtgt cgtacagggt ggccgacgac tggaagctgg cgatgtgccc gcccagctcc
      961 gaggactcct tgttggcccg cagcaccatg gccagcgcgt tccaccggtt cagggagcgg
     1021 atcttgtgct cggtggccag gtcgcccggg taggccggct gctggtcgac cgggatggtg
     1081 ttgaggtagg gcgtggtcgc cgagtacggg gccggcgccc cccgccggcg gccctcggcc
     1141 gacagctcgc cgagcaggaa ccgcgcccgg tcggtcccct cgaacgccag caccgagtcc
     1201 agcgcctcca gccactcacg ggtctcgacc gggtctacgt cgttcagcat ggccatcagt
     1261 ccatccactc aggctggagc accagcgtcc ttcagcaacc gtcggccgat cttaggttct
     1321 agatcgttcc atcaaccgcc ccactcgaac cagtcttgaa ataccccaac tccggccgcg
     1381 cgaacgcatg gtccggcttc ggcattcatc ccatccaggc gatgcgcccc cggtccggag
     1441 gacgcagcct gatcagacaa tgggcatcct cgtggcgttc gccgtcatcg cggcggcggt
     1501 ggcggtcgcg gtcaccgcca tgctgctggt ccgcaggcgt gcccccgaag gcagcttctt
     1561 caacgacggc gaccgcgcct ccggggtgtt cggggtgctc gccaccgggt tcgccgtcct
     1621 cctcggcttc atcgtcgtcc tcgccttcac cagctacgac gagtccaagt cgggggccga
     1681 gctggaggcg ctggccgtgg tccagcagtt cgagacggcc cagttcctgc cggccgccgc
     1741 cggccagcgg gtcgccggcg agctgatctg ctacggacgg tcggtggtgg gccaggagtg
     1801 gccccggatg cagaccggcg ccgagacggg cgccctcaac ccgtggtccg tggccatgtt
     1861 caggacgatg aagacgatcg agccggccac cgcggccgag cagtccgcct acgaccgctg
     1921 gctcgaccag acctccaccc gcgaggaggc ccgcaacgac cgcgtccacg ccgccgaggg
     1981 cgtcatcccc acgcccttgt ggcgggtgct gttcttcagc ggcgggctga tcgtgctcga
     2041 catgctgttc ttcg
//
LOCUS       DASSWR010000339         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_860729,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000339 DASSWR010000000
VERSION     DASSWR010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860729"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VFO47_05635"
     CDS             <1..1093
                     /locus_tag="VFO47_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET8788210.1"
                     /translation="WNPEVREAMDEVMRFWLDRGVDGFRVDVAHKMARDPELRDNPLV
                     DLDEADLDETGEARARRRAKLKSVPRRDEDWPEVHEILRRFRRSLDAYDDRMAVGEVY
                     LLDLERLARYYGSGKDEFHLVHNFVFQSQPWKAEAFRSVVEEFNTLLPPDAWPAWMLS
                     NHDVSRIASRYDEGGNGQARARVAAMLLLTLRGTPFVFMGDEIGQADGEVPPDRVVDV
                     DGRDPQRTPVQWDGSPDAGFGGDPWLPVGPSAATVNVAAQRDDPASMLTLFRRLIWFR
                     KGSAALHGGDYRSLADTPGGCFAYLRTAGDERLLVALNFTGQPQDYQVDGPEEGRLEL
                     STDPARPTGEPTGLRPLRLGPDEGVVVRL"
     gene            complement(1090..2538)
                     /locus_tag="VFO47_05640"
     CDS             complement(1090..2538)
                     /locus_tag="VFO47_05640"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET8788211.1"
                     /translation="MPELSPAQREALGALLNAAPFAGVVRDLGRRFAEGGHELYLVGG
                     TVRDALLDRASPDVDLTTDATPDQILAVVRARNWGDGVWLQGVRYGTVGVRKGDFRLE
                     ITTFRSDTYREDSRKPEVTFGRSIDEDLARRDFTVNAMAMKLPDGPFVDPHGGLGDLA
                     AKRLRTPSSPAVSFEDDPLRMLRAARFASQLELVPTPETLAAITERRARLQIVSRERI
                     RDELSKLLLGRAPAKGLWLAVNTGLAGEFLPELPALALEQDPVHRHKDVLRHTIAVVE
                     RATAFDADEPDLTLRMAALLHDIGKPKTRRVGPEGVTFHLHEVVGAKMAEKRLTELRY
                     PAEFIADVRELVLLHLRFHTYRLGWTDSAVRRYVRDAGPLLDRLNFLVRSDCTTRNVF
                     RARQLAGYSDELEERIARLEAEEELASIRPPLDGRQVMELLGIPPGPLVGKALSNLLE
                     LRLDHGPMSEEEATEALLAWARDQGLEPPPAG"
     gene            2630..2821
                     /locus_tag="VFO47_05645"
     CDS             2630..2821
                     /locus_tag="VFO47_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002822208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788212.1"
                     /translation="MPETKKMSRAEAGRKGGQTTKKRYGDDFFGKIGRMGGKKGGETT
                     KQRYGIEFYQKIGRKGGSR"
     gene            2932..>3282
                     /locus_tag="VFO47_05650"
     CDS             2932..>3282
                     /locus_tag="VFO47_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET8788213.1"
                     /translation="MRSSIDVHNHLLADDVVHELSQLPGPLRDLSSAPGVLGLPPRAV
                     GRPTLLADEDGCVVVLAPADAEVDTSSVAELLGRELDPVPPDRAPGLTGYLLPFVPPV
                     ALECPATLVVDERVA"
BASE COUNT          451 a         1185 c         1211 g          435 t
ORIGIN      
        1 gtggaacccg gaggtccggg aggccatgga cgaggtgatg cgcttttggc tggaccgggg
       61 ggtcgacggc ttccgggtgg acgtggccca caagatggcc cgcgaccccg agctgcgcga
      121 caacccgctg gtcgacctgg acgaggccga cctcgacgag accggcgagg cgagggccag
      181 gcgccgggcc aagctgaagt cggtcccccg ccgcgacgag gactggcccg aggtccacga
      241 gatcctgcgc cgcttccggc ggtccttgga cgcctacgac gaccgcatgg ccgtcggcga
      301 ggtctacctg ctcgacctgg agcgcctggc ccgctactac ggctccggca aggacgagtt
      361 ccacctggtc cacaacttcg tgttccagag ccagccgtgg aaggccgagg ccttccggtc
      421 ggtggtcgag gagttcaaca cgctgctgcc gcccgatgcc tggccggcct ggatgctcag
      481 caaccacgac gtctcccgga tcgccagccg ctacgacgag ggcggcaacg ggcaggcgcg
      541 ggcccgggtg gcggccatgc tcctgctcac cctgcgcggc accccgttcg tcttcatggg
      601 cgacgagatc ggccaggccg acggcgaggt cccgcccgac cgggtggtcg acgtcgacgg
      661 ccgcgacccc cagcgcaccc ccgtccagtg ggacggctcc cccgacgccg gcttcggcgg
      721 ggacccgtgg ctgcccgtcg gtccgagtgc ggccacggtc aacgtggccg cccagcgcga
      781 cgacccggcg tccatgctga ccctgttccg gcgcctgatc tggttccgca agggctcggc
      841 cgccctccat gggggcgact accgctccct ggccgacacc cccggcggct gcttcgccta
      901 cctgcgcacg gccggcgacg agcgcctgct ggtcgccctc aacttcaccg gccagcccca
      961 ggactaccag gtcgacggcc ccgaggaggg ccgcctggag ctctccaccg acccggcccg
     1021 ccccaccggc gagccgaccg gcctgcggcc cctgcgcctg ggtcccgacg agggtgtggt
     1081 cgtccggctc tagccggccg gcggcggctc cagaccctgg tcgcgggccc aggccaggag
     1141 ggcctcggtg gcctcctcct cggacatggg gccgtggtcg aggcgcagct ccagcaggtt
     1201 ggacagggcc ttgccgacca gggggccggg cgggatgccg agcagctcca tcacctggcg
     1261 gccgtccagc ggcggccgga tgctggccag ctcctcctcg gcctcgaggc gggcgatccg
     1321 ctcctcgagc tcgtcggagt agccggccag ctgccgcgcc ctgaacacgt tgcgggtggt
     1381 gcagtcggag cggaccagaa agttgagccg gtccagcagc ggcccggcgt cgcgcacgta
     1441 gcgccgcacc gccgagtcgg tccagcccag ccggtaggtg tggaaccgca ggtggagcag
     1501 gaccagctcg cgcacgtcgg cgatgaactc cgccgggtag cgcagctcgg tcagccgctt
     1561 ctcggccatc ttggccccga ccacctcgtg gaggtggaag gtcacgccct ccggcccgac
     1621 ccggcgggtc ttgggcttgc cgatgtcgtg gagcagggcg gccatgcgca gggtcaggtc
     1681 gggctcgtcg gcgtcgaagg cggtcgcccg ctcgaccacc gcgatggtgt gccgcagcac
     1741 gtccttgtgc cggtggaccg ggtcctgctc cagggccagg gcgggcagct cgggcaggaa
     1801 ctcgccggcc aggccggtgt tgacggccag ccacaggccc ttggccgggg cccggcccag
     1861 cagcagcttg ctcagctcgt cgcggatgcg ctcgcgggag acaatctgca ggcgggcgcg
     1921 gcgctcggtg atggcggcca gggtctcggg agtggggacg agctcgagct gggaggcgaa
     1981 acgggccgcc cgcagcatcc gcagcgggtc gtcctcgaac gacaccgccg ggctggacgg
     2041 ggtgcgcagg cgcttggcgg ccaggtcgcc cagtccgccg tgggggtcga cgaacgggcc
     2101 gtcgggcagc ttcatggcca tggcgttgac ggtgaagtcg cgccgggcca ggtcctcgtc
     2161 gatggaccgg ccgaaggtga cctcgggctt gcgcgagtcc tcgcggtagg tgtcggagcg
     2221 gaaggtggtg atctccagcc ggaagtcgcc cttgcggacc ccgacggtgc cgtagcgcac
     2281 cccctgcagc cacaccccgt cgccccagtt gcgggccctg acgacggcca ggatctggtc
     2341 gggcgtggcg tcggtggtca ggtcgacgtc cggcgaggcc cggtcgagca gggcgtcgcg
     2401 gaccgtgccg ccgaccaggt acagctcgtg cccgccctcg gcgaagcgcc gcccgaggtc
     2461 gcggacgacg ccggcgaacg gggccgcgtt cagcagcgcg cccagcgcct cgcgctgggc
     2521 cggggagagc tctggcatgt cccgcaggat acgcgcgtcc atgccggacc agttcaagtg
     2581 gtttacgcac caacaaccag ggcaaccgct ttggggagga ggtggtcgga tgccggagac
     2641 caagaagatg agcagggccg aggccggacg taagggcggc cagaccacca agaaacgcta
     2701 cggcgacgac ttcttcggga agatcggccg gatgggcggg aagaagggcg gcgaaaccac
     2761 caagcagcgc tacgggatcg agttctacca gaagatcggc cgcaagggcg gatcgcgctg
     2821 actgggagcc ctcacgtcgt tgggggtgtc gaccgaggcc ggggctgaag ctccggcctc
     2881 ccgctatact cgggtccgct cgaccgccgc gctcgcatgc ctggagaccc cgtgcgatca
     2941 agcatcgacg tccacaacca cctgctcgcg gacgacgtcg tgcacgagct ctcccagctc
     3001 ccgggaccgc tccgcgacct gtcgtccgcg ccgggcgtcc tcggcctgcc gccccgggcc
     3061 gtcggccggc ccacgctgct ggccgacgag gacgggtgcg tggtcgtgct cgccccggcc
     3121 gacgccgagg tcgacacctc cagcgtggcc gagctgctcg gccgcgagct cgaccccgtc
     3181 ccgcccgacc gggcccccgg cctgaccggg tacctgctgc cgttcgtccc gccggtcgcc
     3241 ctggagtgcc cggccaccct ggtggtcgac gagcgggtgg cc
//
LOCUS       DASSWR010000340         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1002349, whole genome shotgun sequence.
ACCESSION   DASSWR010000340 DASSWR010000000
VERSION     DASSWR010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002349"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFO47_05655"
     CDS             complement(<1..1252)
                     /locus_tag="VFO47_05655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET8788214.1"
                     /translation="MRVTVIGAGHVGLVTAACLAHVGHDVVVDDDDVSKLDLIREGRS
                     WFYEPGLQDLLGEVVRSGRLRVVDDKAEAVEHGTVIFICVGTPSRGDGSPNLAYVEAV
                     AREVARSLPPGEFRLICEKSTVPVQTGERVARVIAREARPGADWEVASNPEFLREGSA
                     VVDTLDPDRVVVGTSSERGLAALRELYQPILERSGAPFLATDRATAELIKHASNAFLA
                     TKISFINSVARVCERSGADVELVARGMGLDPRIGVHFLKAGAGYGGSCFPKDVAAFAH
                     RSRELGVDFGILNEVAKINHEARRAVVDKVRDALWHLDGKRIGMLGLSFKPNTDDLRE
                     APAIDVARDLLADGAQVVAYDPVAGEAAARQVPGLELADKAVGVADGAHAIVLMTEWA
                     EFGELDPADLSDRMTYPILVDARNA"
     gene            1379..>2115
                     /locus_tag="VFO47_05660"
     CDS             1379..>2115
                     /locus_tag="VFO47_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET8788215.1"
                     /translation="MGPRPALPLRLCRLLLVATVLGAGVVPGWAGRAEAAPPAPAGPE
                     AVRARYAILVDPASGAVLWGRRSRTPAPPASLTKMLTALAVRASLELDAVTVASKAAA
                     GQPARRLAVRRGQKLTVDQALKALMISSANDVAVMLAEAAGGSVGRFAKAMDAESERL
                     GLRGSSWRNPHGLDQRGHRSTAFDLAILARAVLGDRWLARVVRKRHAAFVTPSGRRHS
                     LTARSRFLLGYRGAVGVKTGFTDDAGH"
BASE COUNT          257 a          782 c          788 g          288 t
ORIGIN      
        1 ccgcgttgcg ggcgtcgacc aggatcgggt aggtcatgcg gtcggagagg tcggccgggt
       61 ccagctcgcc gaactcggcc cactcggtca tcagcacgat ggcgtgggcg ccgtcggcca
      121 ccccgaccgc cttgtcggcc agctccaggc cggggacctg ccgggccgcc gcctcgccgg
      181 ccaccgggtc gtaggcgacc acctgggcgc cgtcggccag caggtcgcgg gccacgtcga
      241 tcgccggggc ctcgcgcagg tcgtcggtgt tgggcttgaa cgacagcccc agcatcccga
      301 tccgcttgcc gtccaggtgc cagagggcgt cgcggacctt gtcgaccacg gcccgccggg
      361 cctcgtggtt gatcttggcc acctcgttga ggatgccgaa gtcgaccccc agctcgcggg
      421 agcggtgggc gaaggcggcc acgtccttgg ggaagcagct cccgccgtag ccggcgccgg
      481 ccttgaggaa gtggacgccg atgcgcgggt cgaggcccat gcccctggcc accagctcga
      541 cgtccgcccc cgaccgctcg cacaccctgg ccaccgagtt gatgaagctg atcttggtgg
      601 ccaggaaggc gttggaggcg tgcttgatca gctcggcggt ggcccggtcg gtggccagga
      661 acggcgcccc cgagcgctcc aggatcggct ggtagagctc gcggagggcg gccaggcccc
      721 gctcggagct ggtgccgacg accacgcggt ccgggtcgag ggtgtcgacc acggccgagc
      781 cctcgcgcag gaactcgggg ttggaggcga cctcccagtc ggccccgggc ctggcctcgc
      841 gggcgatgac cctggccacc cgctcgccgg tctgcaccgg cacggtggac ttctcgcaga
      901 tcaggcggaa ctcccccggg ggcaggctgc gggcgacctc gcgggccacc gcctcgacat
      961 aggccaggtt ggggctgccg tcgccgcggc tcggggtgcc gacgcagatg aagatcacgg
     1021 tcccgtgctc gacggcctcg gccttgtcgt cgaccacccg gagccgcccc gaccggacca
     1081 cctcgcccag caggtcctgc aggccgggct cgtagaacca ggagcggccc tcccggatca
     1141 ggtcgagctt gctgacgtcg tcgtcgtcga ccacgacgtc gtggccgaca tgggcaaggc
     1201 aggcggcggt gacgaggccg acgtggccgg cgccgatcac ggtgacgcgc atactggcaa
     1261 gcttcccccg ggtctccact gatgagatcg cgccggacga ttctagtgga tggccgccga
     1321 ttcggaaccg agcgaccatc ttgtccggct cgtctgccgg tgcgacactg cggcagccat
     1381 gggtccgcgc cccgccctgc cgctccggct ctgccgcctg ctcctggtcg ccaccgtcct
     1441 gggggccggg gtcgtgccgg ggtgggctgg ccgggccgag gccgccccgc cggcgcccgc
     1501 ggggccggag gcggtccggg ccaggtacgc gatcctggtc gacccggcga gcggggcggt
     1561 gctctggggg cggcggagcc gcacgcccgc gcccccggcc agcctgacca agatgctgac
     1621 cgccctggcc gtccgggcca gcctggagct ggacgccgtg accgtggcct ccaaggcggc
     1681 ggccggccag ccggcccggc gcctggccgt gcggcgcggc cagaagctca ccgtcgacca
     1741 ggccctcaag gccctgatga tctcctcggc caacgacgtg gcggtgatgc tggccgaggc
     1801 ggccggtggg tcggtgggcc ggttcgccaa ggccatggac gccgagtcgg agcggctcgg
     1861 cctgcgggga tcgtcctggc gcaacccgca cggccttgac cagcgcgggc accgctcgac
     1921 cgcgttcgac ctggccatcc tcgctcgggc ggtgctcggt gaccgctggc tggcccgggt
     1981 cgtgcgcaag cgccatgccg ccttcgtgac cccgagcggc cgccggcact cgctcaccgc
     2041 ccgctcccgg ttcctgctcg gctaccgggg cgcggtcggg gtcaagaccg ggttcaccga
     2101 cgacgccggc cactg
//
LOCUS       DASSWR010000341         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1256352, whole genome shotgun sequence.
ACCESSION   DASSWR010000341 DASSWR010000000
VERSION     DASSWR010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256352"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFO47_05665"
     CDS             complement(<1..789)
                     /locus_tag="VFO47_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017611270.1"
                     /note="similar to DNA polymerase I; lacks 5'-3'
                     exonuclease domain; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HET8788216.1"
                     /translation="MRVAVVEDAEGGGLLQAVAEDGTPQRPVERVADLAAAVAERERT
                     GPRWVWAATGDVYPRLLAAGVRVGRCHDLGLTEQLLLAADGRWGEPRSFPAAWARLRG
                     LPVPDDRAPGLARTGQPALFDHDLLGLPAGPELLEGVVAVHADQQHRLAGAVPRMIGP
                     GGLDGLGLLVAAESAGALAAAEMAAAGLPWRPEVHDAVLTELLGPRPRAGNVPPRLAD
                     LAARIGAAFGGQPLNPDSPAQLLRAFATAGLPLPSTRSHVLREVD"
     gene            complement(828..>2039)
                     /locus_tag="VFO47_05670"
     CDS             complement(828..>2039)
                     /locus_tag="VFO47_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET8788217.1"
                     /translation="LVDQGRSYEEARRLVRQHYQHIVLHDFLKRVADPAIVDGVISGG
                     NRFYDALGEPFFLPLEVTVAGFRFGHTMVRDTYDFNLNFDPASLGLLFTFTALSGELG
                     DFDTLPDNWIIQWERFIAPGGGKNKARRFDTKLADGLFQLQTVTGQLEQPADAANLAV
                     RNLLRGYGLRMPTGQALAGRLGLPALTAAQLEAAAASTRQAKVLRDAGFLERTPLWYY
                     LLAEARHGGGQRLGPVGGTLVAEVLVGLVRRSEDSILRTPGWVPSLPAATAGRFELAD
                     LLRFAKVAGGGAAPRTYKVKKGDSLSLIAQKQLGDAGRWPELFVLNRAKIRHRDRLSV
                     GQVLTLPGPTPIKPRPQLYKVRKGDTLSGIAKSKLGDASRWPEIAKLNRDVVPDPDEI
                     APGLVLVIVKG"
BASE COUNT          251 a          761 c          783 g          244 t
ORIGIN      
        1 gtcgacctcg cgcagcacgt gcgatcgggt cgagggcagc ggcaggccgg cggtggcgaa
       61 ggcccgcagc agctgggcgg gcgagtcggg gttgagcggc tgcccgccga acgcggcccc
      121 gatccgggcg gccaggtcgg ccagccgcgg cggcacgttc ccggccctcg gccgggggcc
      181 gagcagctcg gtgaggaccg cgtcgtgcac ctcgggccgc cacggcagcc cggccgcggc
      241 catctcggcg gcggccagcg cccccgccga ctccgccgcc accagcagcc cgagcccgtc
      301 cagtcctccc gggccgatca tccgggggac cgccccggcc agccggtgct gctggtcggc
      361 gtggacggcc acgaccccct cgagcagctc gggcccggcg ggcagcccca gcaggtcgtg
      421 gtcgaacagg gccggctggc cggtcctggc cagccccggg gcccggtcgt cgggcaccgg
      481 caggccgcgc aggcgggccc aggcggccgg gaacgagcgc ggctcgcccc agcggccgtc
      541 ggcggcgagc aggagctgct cggtcaggcc caggtcgtgg cagcgcccga cccgcacccc
      601 ggccgccagc agccgcgggt agacgtcccc ggtggccgcc cacacccagc gcggcccggt
      661 ccgctcccgc tccgccaccg ccgcggccag gtcggccacc cgctccaccg gccgctgggg
      721 cgtgccgtcc tcggcgacgg cctgcagcag ccccccaccc tcggcgtcct ccaccaccgc
      781 gacccgcatg ccgggaagtc tgcccgcccc cagcgacagc cggccggtca gcctttgacg
      841 atgaccagca ccagcccggg ggcgatctcg tcggggtcgg ggacgacgtc gcggttgagc
      901 ttggcgatct ccggccagcg gctcgcgtcg ccgagcttgc tcttggcgat gccggagagg
      961 gtgtcgccct tcctgacctt gtacagctgg ggccggggct tgatcggggt gggcccgggc
     1021 agggtcagga cctggccgac gctgagccgg tcgcggtggc ggatcttggc ccggttgagc
     1081 acgaacagct cgggccagcg accggcgtcg ccgagctgct tctgggcgat cagggagagg
     1141 ctgtcgccct tcttgacctt gtaggtccgg ggcgcggccc cgcccccggc gaccttggcg
     1201 aaccgcagca ggtcggccag ctcgaaccgg cccgccgtgg ccgcgggcag ggacggaacc
     1261 cagccgggcg tgcgcaggat cgagtcctcg ctgcgccgga ccagcccgac cagcacctcg
     1321 gcgaccaggg tgccgccgac cgggccgaga cgctggccgc cgccgtggcg ggcctcggcc
     1381 agcaggtagt accagagcgg cgtccgctcc aggaacccgg cgtcgcgcag caccttggcc
     1441 tggcgggtgc tggccgcggc cgcctcgagc tgggccgcgg tcagggccgg cagccccagc
     1501 cgcccggcca gggcctgccc ggtgggcatg cgcagcccgt agccgcgcag caggttgcgg
     1561 acggccaggt tggcggcgtc ggccggctgc tccagctggc cggtgaccgt ctggagctgg
     1621 aacaggccgt cggccagctt ggtgtcgaac cgccgggcct tgttcttgcc gccgccgggg
     1681 gcgatgaagc gctcccactg gatgatccag ttgtcgggca gggtgtcgaa gtcgcccagc
     1741 tcgccgctga gggcggtgaa ggtgaacagc agccccagcg acgccgggtc gaagttgagg
     1801 ttgaagtcgt aggtgtcgcg gaccatggtg tgaccgaagc ggaacccggc cacggtgacc
     1861 tcgagcggca ggaagaacgg ctcgccgagg gcgtcgtaga agcggttgcc gccgctgatg
     1921 accccgtcca cgatcgccgg gtcggcgacc cgcttgagga agtcgtggag cacgatgtgc
     1981 tggtagtgct ggcggaccag ccgccgcgcc tcctcgtagc tgcggccctg gtcgaccag
//
LOCUS       DASSWR010000342         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_860775,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000342 DASSWR010000000
VERSION     DASSWR010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860775"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFO47_05675"
     CDS             complement(<1..851)
                     /locus_tag="VFO47_05675"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIB"
                     /protein_id="HET8788218.1"
                     /translation="MKIVAVTSCPTGIAHTYMAAEALEQAAKDAGHDIKVETQGAAGA
                     EAVADADITGAEVVVFAADVEVRNRERFAGKPLVQTSVKRAINDAPGLIAEAEAAART
                     GSAGGVMAGAAAEAQESVAMETAARPETKIDQSAGLGTRLRQWLMTGVSYMIPFVAAG
                     GILIALAIVFGGGEVISKVNGGTYEGVTYEKITDYSKLISQAGWPGLMFFIGATAFSM
                     LVPILAGFIAYAMGDRPALVAGIVAGLISVEISAGFLGGLIGGLLAGAVVIAIKRVKV
                     PRGMAGI"
     gene            complement(848..1300)
                     /locus_tag="VFO47_05680"
     CDS             complement(848..1300)
                     /locus_tag="VFO47_05680"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HET8788219.1"
                     /translation="MADLITPDLVDLNLTQTDRGAATRALAERLQAAGRVTDLEQFLA
                     DVRAREAQMPTGIEGGIGIPHCRSVAVVEPTLAFGRSAQGIDWGADDGPAHLIFLIAA
                     PEGGGAEHMGILAKLARKLIHPSFRDSLHQAPDPEAVVSLVKKEVLGQ"
     gene            complement(1306..2250)
                     /gene="pfkB"
                     /locus_tag="VFO47_05685"
     CDS             complement(1306..2250)
                     /gene="pfkB"
                     /locus_tag="VFO47_05685"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET8788220.1"
                     /translation="MIVTLTPNPSVDRTIEVDALHRGAVLRARATRVDPGGKGVNVSR
                     ARAANGRETTAVLPAGGWEGEQLAALLAPLDVPVVVVPIAGSVRANVSVVEPDGTVTK
                     LNEPGPRLSAEEIEAVTAATVKAAAEAEWLACTGSLPPGAPIDFYARVVERLGGAPVK
                     VAIDSSGAPLVAALDAGPDLIKPNREELAEATGRSLLTVGDVVAAAEELRGRGVGAVL
                     ASLGADGAVLVDATGATFGSATVERPRSAVGAGDSMLAGFLAGGGQGPDALAEALAWG
                     AGAASLPGSRMPTPDDLDRGAVTLADRIDPDRPLKERS"
     gene            complement(2247..3008)
                     /locus_tag="VFO47_05690"
     CDS             complement(2247..3008)
                     /locus_tag="VFO47_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET8788221.1"
                     /translation="MFAEQRQQRIVEQARQAGRVDVAELAAELAVTPETVRRDLTALE
                     RQGVLRRVHGGAMPVERLGFEPGLAARDAVRRAEKERIAKAALSELPVEGSILLDAGT
                     TTARLAEILPATRDLTVVTNSLPIAIGLSVRPGLTVLMTGGRVRGRTQAAVGEGAVRV
                     LGDTFVDVAFLGTNGITPERGLTTPDPDEAAVKRAMLAAARRVVVLADHTKVGATHFA
                     RFGDLREVAILITDSGLDADVAARLEAAGPTVVRA"
BASE COUNT          385 a         1138 c         1185 g          448 t
ORIGIN      
        1 atgatgccgg ccatgccccg tggcaccttg acccgcttga tggcgatcac gaccgcgccg
       61 gccagcaggc cgccgatcag gccgccaagg aacccggcgc tgatctcgac cgagatcagg
      121 ccggcgacga tgccggcgac cagggcgggc cggtcgccca tggcgtaggc gatgaagccg
      181 gctaggatcg gcaccagcat ggagaaggcg gtcgcgccga tgaagaacat cagccccggc
      241 cacccggcct ggctgatgag cttggagtag tcggtgatct tctcgtaagt gacgccttcg
      301 taggtgccgc cgttgacctt gctgatgacc tcgccgccgc cgaagacgat ggccagggcg
      361 atcaggatgc cgccggcggc cacgaacggg atcatgtagc tgaccccggt catcagccac
      421 tggcgcaggc gggtgccgag gccggccgac tggtcgatct tggtctcggg ccgggcggcc
      481 gtctccatgg ccaccgattc ctgggcctcg gcggccgcgc cggccatcac gccgcccgcg
      541 ctcccggtcc tggcggccgc ctcggcctcg gcgatcaggc cgggggcgtc gttgatggcc
      601 cgcttgaccg aggtctggac cagcggcttg cccgcgaacc gctcgcggtt gcggacctcg
      661 acgtcggccg cgaacaccac cacctcggcg ccggtgatgt cggcgtcggc caccgcctcg
      721 gccccggccg cgccctgggt ctcgaccttg atgtcgtgcc cggcgtcctt ggcggcctgc
      781 tccagggctt cggccgccat gtaggtatgg gcgatccctg tggggcagga cgtcacggcg
      841 acgatcttca ttgccccagc acctccttct tcaccaggct gacgactgcc tcagggtccg
      901 gggcctggtg cagggagtcc cggaacgagg ggtggatgag cttccgggcc agcttggcca
      961 ggatgcccat gtgctcggcg ccgccgccct cgggggcggc gatcaggaag atgaggtggg
     1021 ccggcccgtc gtcggccccc cagtcgatgc cctgggcgct gcggccgaag gccagcgtcg
     1081 gctcgaccac ggccaccgac cggcagtgcg ggatgccgat gccgccctcg atgccggtcg
     1141 gcatctgggc ctcgcgggcg cgcacgtcgg ccaggaactg ctcgaggtcg gtgacccgcc
     1201 cggccgcctg gagccgctcg gcgagggctc gggtcgccgc accgcggtcg gtctgggtca
     1261 ggttgaggtc gaccaggtcg ggggtgatga ggtccgccat gggcgtcagc tccgttcctt
     1321 gagagggcgg tccgggtcga tgcggtcggc cagggtgacc gcgccgcggt cgaggtcgtc
     1381 gggggtcggc atgcggctgc cgggcaggct ggccgcgccc gcgccccagg ccagggcctc
     1441 ggccagggcg tcggggccct ggccgccgcc ggccaggaag ccggccagca tggagtcgcc
     1501 ggcgccgacg gcgctgcggg gccgctccac ggtggccgag ccgaaggtgg cgccggtggc
     1561 gtcgaccagc accgccccgt ccgccccgag gctggccagc acggccccga ccccccggcc
     1621 gcgcagctcc tcggccgcgg cgaccacgtc gccgacggtg aggagcgacc ggccggtggc
     1681 ctcggccagc tcctcgcggt tgggcttgat caggtcgggg ccggcgtcga gggcggccac
     1741 caggggggcg ccgctggagt cgatcgccac cttgaccggg gcgccgccca gccgttcgac
     1801 cacgcgggcg tagaagtcga tcggggcgcc cggcgggagg ctgcccgtgc aggccagcca
     1861 ctcggcctcg gcggcggcct tgacggtggc cgcggtcacg gcctcgatct cctcggcgct
     1921 gagccgcggg ccgggctcgt tgagcttggt cacggtgccg tcgggctcga ccacgctcac
     1981 gttggcccgg accgacccgg cgatggggac gaccaccacc gggacgtcga gcggggcgag
     2041 cagggcggcc agctgctcgc cctcccagcc gccggccggg agcacggccg tggtctcgcg
     2101 gccgttggcg gcccgggccc gtgacacgtt gacgcccttg ccgccggggt cgacccgggt
     2161 ggcccgggcc cgcagcaccg ccccccggtg cagggcgtcg acctcgatgg tccggtcgac
     2221 gctcgggttg ggggtgaggg tcacgatcat gcgcgcacca ccgtgggtcc ggccgcctcc
     2281 agccgggcgg cgacgtcggc gtccagtccg gagtcggtga tgaggatagc cacctcgcgc
     2341 aggtctccaa acctggcgaa gtgggttgct cccaccttgg tgtggtcggc cagcacgacc
     2401 acccgccggg cggcggccag catggcccgc ttgacggccg cctcgtccgg gtcgggagtg
     2461 gtcaggcccc gctcgggcgt gatgccgttg gtgccgagga aggccacgtc gacgaaggtg
     2521 tcgccgagca cccgcacggc cccctccccg accgccgcct gggtgcggcc gcggacccgg
     2581 ccgccggtca tgagcacggt caggccgggg cggaccgaca ggccgatggc gatcggcagg
     2641 ctgttggtga ccaccgtcag gtcgcgggtg gccgggagga tctcggccag gcgggcggtg
     2701 gtggtgccgg cgtcgagcag gatcgagccc tcgaccggga gctcggagag ggccgccttg
     2761 gcgatccgct ccttctcggc ccgccggacc gcgtcccggg cggccaggcc gggctcgaag
     2821 ccgagccgct cgaccggcat ggccccgcca tggacccgcc ggagcactcc ctgccgctcc
     2881 agcgcggtca ggtcacggcg cacggtctcg ggcgtcacgg cgagctcggc cgccagctcg
     2941 gccacgtcca cccggccggc ctggcgtgcc tgttcgacga tgcgctgctg acgctgctcg
     3001 gcgaacatgt ttgctcctgt ccgtcttctt ttgttttgtt ttgtataagc ccgtttctgt
     3061 ccgttgtcaa gattggttga acgatatata tccccgcgat cgagccaagg taagcccgta
     3121 ggtcagaggc cgacccacca gccgaccccg gcaacc
//
LOCUS       DASSWR010000343         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_746968,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000343 DASSWR010000000
VERSION     DASSWR010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746968"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..502
                     /locus_tag="VFO47_05695"
     CDS             143..502
                     /locus_tag="VFO47_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HET8788222.1"
                     /translation="MRLVDYTLAKRAKLTELRGGRLSRDDVCDAHPELLRAARNVGEQ
                     VQTPCPVCASEPLVHVTYVFGDALRQANGRVWPRTGLADLHRSMDEVACYVVEVCTAC
                     YWNHLAQRYLLGRRHVG"
     gene            543..>2537
                     /locus_tag="VFO47_05700"
     CDS             543..>2537
                     /locus_tag="VFO47_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET8788223.1"
                     /translation="MAAPPITPTRAARHQQAKPQGRERKQAPPNRSRVRRILRWTLWL
                     AVPAVAVAAGTVVGLIYAFARVPLPEEVPTAQTIVFLDESGKKEIGTLSAQENRRVVL
                     LAKIPEHTRRAVLAAEDRDFYEHGAISWRGLARASAANLLRRRISEGGSTITQQYVKN
                     AFPGVGRDRTLFRKLKEAVLAVKLERKFSKDKILEFYLNTVYFGRGAYGIDAAARTYF
                     KKKAESLEPAQSALLAGVIRSPEFYGKKEHQASAKARRDYILQAMVDRGWLTAKDGRA
                     AIATKPGISWKATRGGIANSDAPFFLEKVRQYLVARYGAEAVNLGGLRVRTTLDTEMQ
                     DLAEATVTRILDDPKTNPKAALVSIDPRNGAVRAMYGGRNFKGRQFNYATNSIRQAGS
                     TMKPFVLAQALADGISVNEVFPGPAELIVDGEKFENYGDTNYGQLTLRDATRLSVNTI
                     YVQLMQMVQSDRVAKLAKAHGLTAELGGPGEPGRDPRPLEDAPVLKPVLALSLGSGDV
                     TTLQLASAFGTWANRGIHHAPHLVEKVVDFRGRVLEEHGRQQGAQVLSRVHADIMNMV
                     LKGAVENGTGSAARLPGREVAGKTGTTSNYADARFVGYTTDLVTSVWVGYDDQKHKLV
                     DVPGYPGGVSGGSLPAQIWHDYMDLATSDRPSLPFEPPAEI"
BASE COUNT          418 a          943 c          834 g          342 t
ORIGIN      
        1 tggttcacta ctgcaggccg agccgatgta tcgactcgat gtatcgaaac gatagatacg
       61 cctacgcgag tatgtcaact cgcgttgccg agccgttatc gctccagtgc cacgagaatg
      121 ggccgggtac actggcgccg agatgcggct agtcgactac accctggcca agcgggcgaa
      181 gctcaccgag ctccgcggcg gccgcctgtc gcgcgacgac gtctgcgacg cccaccccga
      241 gctgctgcgg gccgcccgca acgtcggcga gcaggtccag acgccctgcc cggtttgcgc
      301 ctccgagccg ctggtccacg tcacctacgt gttcggcgac gccctgcgcc aggccaacgg
      361 ccgtgtctgg ccccgcaccg gcctcgccga cctgcaccgc tccatggacg aggtggcctg
      421 ctacgtggtc gaggtctgca ccgcctgcta ctggaaccat ctcgcccagc ggtacctgct
      481 gggccgccgc cacgtcggct gaatcggctt ccggccaacg gttggagtaa tcttctcgcg
      541 ctatggcagc acccccgatc acccccacac gggccgccag gcaccagcag gccaagccgc
      601 agggcaggga gcgcaagcag gcacccccca accgctcccg cgtccgccgg atcctgcgct
      661 ggacgctgtg gctggccgtg ccggccgtgg ccgtggccgc cggcaccgtc gtcgggctga
      721 tctacgcctt cgcccgggtg cccttgcccg aggaggtgcc gaccgcccag acgatcgtgt
      781 tcctcgacga gtccggcaag aaggagatcg gcaccctctc ggcccaggag aaccgccggg
      841 tcgtcctcct ggcgaagatc cccgagcaca cccgccgggc ggtgctggcc gccgaggacc
      901 gcgacttcta cgagcacggg gccatctcct ggcgcgggct ggccagggcg agcgcggcca
      961 acctgctgcg ccggcgcatc tccgagggcg gctccaccat cacccagcag tacgtgaaga
     1021 acgccttccc cggcgtgggc cgcgaccgga ccctgttccg caagctcaag gaggccgtgc
     1081 tcgcggtcaa gctggagcgc aagttctcca aggacaagat cctcgagttc tacctgaaca
     1141 cggtctactt cggccggggc gcctacggca tcgacgccgc cgcccgcacc tatttcaaga
     1201 agaaggccga gagcctcgag ccggcccagt cggccctgct cgccggcgtg atccgcagcc
     1261 ccgagttcta cggcaagaag gagcaccagg cctcggccaa ggcgcggcgc gactacatcc
     1321 tccaggcgat ggtcgaccgc ggctggctga cggccaagga cgggcgggcc gccatcgcca
     1381 ccaagcccgg gatctcctgg aaggccacgc gcggcgggat cgccaactcc gacgccccct
     1441 tcttcctgga gaaggtccgc cagtacctgg tcgcccgcta cggcgccgag gccgtcaacc
     1501 tgggcgggct gcgggtccgg accaccctcg acacggagat gcaggacctg gccgaagcca
     1561 cggtcacgag gatcctggac gacccgaaga ccaaccccaa ggcggcgctg gtctcgatcg
     1621 acccccggaa cggggcggtg cgggccatgt acggcggccg caacttcaag ggccgccagt
     1681 tcaactacgc caccaactcg atccgccagg ccggctcgac catgaagccg ttcgtgctcg
     1741 cccaggcgct ggccgacggc atctcggtca atgaggtgtt ccccggcccg gcggagctga
     1801 tcgtcgatgg cgagaagttc gagaactacg gcgacaccaa ctacggccag ctgactctgc
     1861 gggacgccac caggctgtcc gtcaacacca tctacgtgca gctcatgcag atggtgcagt
     1921 ccgaccgggt ggccaagctg gccaaggcgc acggcctgac ggccgagctc ggcggtccgg
     1981 gcgagcccgg ccgggacccg cggccgctgg aggacgcgcc cgtgctcaag ccggtcctcg
     2041 ccctgtccct cggctccggg gacgtgacca ccctccagct cgcctcggcc ttcggcacct
     2101 gggccaaccg cggcatccac catgccccgc acctggtcga gaaggtggtc gacttcaggg
     2161 gccgggtgct ggaggagcac ggcaggcagc agggcgcaca ggtgctcagc cgcgtgcacg
     2221 ccgacatcat gaacatggtc ctgaagggcg cggtcgagaa cggcaccggc agcgcggccc
     2281 ggctgcccgg ccgcgaggtg gccggcaaga cgggcacgac cagcaactac gccgacgccc
     2341 ggttcgtcgg ctacaccacc gacctggtca cctcggtctg ggtcggctac gacgaccaga
     2401 agcacaagct ggtcgacgtc cccgggtacc ccgggggcgt gtccggcggg tcgctgccgg
     2461 cccagatctg gcatgactac atggacctgg ccacgagcga ccggcccagc ctcccgttcg
     2521 agcccccggc cgagatc
//
LOCUS       DASSWR010000344         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_860801,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000344 DASSWR010000000
VERSION     DASSWR010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860801"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="tsaD"
                     /locus_tag="VFO47_05705"
     CDS             complement(<1..811)
                     /gene="tsaD"
                     /locus_tag="VFO47_05705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET8788224.1"
                     /translation="MLVLGIETSCDETGVALVSDGRLVRSNVLADQQTLHARYGGVVP
                     EVASRAHVDLLHPAVEQACALGGARLSDVDAVAVTVGPGLAGSLLVGVAAAKAYALAL
                     DRPLVGVNHLIAHVYANVLDHGTLPLPAVVFVVSGGHTSLLAMDEQGAFRGLGQTIDD
                     AAGQAYDKVARVLGLGYPGGPVVDRLARDGDPAAVRFPRALLDDGSDDFSFSGLKTAV
                     LRHVRAAGPDLDVADVAASFQEAVVDVQISKVIGVAKREGLDTILLGGGVAA"
     gene            complement(814..1377)
                     /gene="rimI"
                     /locus_tag="VFO47_05710"
     CDS             complement(814..1377)
                     /gene="rimI"
                     /locus_tag="VFO47_05710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET8788225.1"
                     /translation="MATIYPPRRQVDDPRQPLAIELVPMRRRHIPQVLDIERQVYPRP
                     WTMTLFLSEIVQRSTRFYIVAKARRQVVGYAGLMVFGDEAHVTNIAVDPAAHRRKVAS
                     RLLFALVTEARRRGATACTLEVRVANHAAQGLYHQFGFAPVGIRKNYYAETGEDALIM
                     WAEGLQAPAYGERLAGLAARIPEPREL"
     gene            complement(1377..2075)
                     /gene="tsaB"
                     /locus_tag="VFO47_05715"
     CDS             complement(1377..2075)
                     /gene="tsaB"
                     /locus_tag="VFO47_05715"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET8788226.1"
                     /translation="MDTATPVTGVAVGSEAGTLAEVAVRHERRHAEVLVPAVRWVLEQ
                     AEVDPGALAGVAVGTGPGLFTGLRVGVSTAKALAQAWGLPMVAVPSLDLIAFAHRHVR
                     RAICPVIDARRGEVFCALYRHAPGGVVRITDYQVLRPDQLAAGIEARGEELLLCGDGA
                     LLYREAFEHLGEQSLIGGLTQSSPSAAALVELALPRMLREEFTSPLDVTPLYLRRPDI
                     DPNVERRLASGGAA"
BASE COUNT          316 a          810 c          853 g          263 t
ORIGIN      
        1 tggcggccac gcccccgccg agcaggatcg tgtccaggcc ctcgcgcttg gccacgccga
       61 tgaccttgct gatctggacg tccaccaccg cctcctggaa cgaggcggcc acgtcggcca
      121 cgtccaggtc cggcccggcc gcccggacgt ggcggagcac ggccgtcttg aggccggaga
      181 aggagaagtc gtcggagccg tcgtccagca gggccctggg gaaccgcacc gcggccgggt
      241 cgccgtcgcg ggccaggcgg tccaccaccg ggccgcccgg gtagccgagg ccgagcaccc
      301 gggccacctt gtcgtaggcc tggccggcgg cgtcgtcgat ggtctggccg agaccccgga
      361 acgccccctg ctcgtccatg gccagcaggc tggtgtgccc gcccgagacc acgaacacca
      421 ccgccggcag gggcagggtc ccgtggtcga gcacgttggc gtagacgtgg gcgatcaggt
      481 ggttgacccc gaccaggggc cggtcgaggg cgagggcgta ggccttggcc gccgccaccc
      541 cgacgagcag ggagccggcc aggccggggc cgacggtgac cgcgaccgcg tcgacgtcgg
      601 acaggcgggc cccaccgagg gcgcaggcct gctcaacggc cgggtggagc aggtcgacgt
      661 gggcccggga ggcgacctcg ggcaccaccc cgccgtagcg ggcgtgcagg gtctgctggt
      721 cggcgagcac gttggagcgg accaggcggc cgtcggagac cagggcgacg ccggtctcgt
      781 cgcaggaggt ctcgatcccg agcaccagca ccgctacagc tcccggggct cggggatgcg
      841 ggcggccagg ccggccaggc gctcgccgta ggccggggcc tggaggccct cggcccacat
      901 gatcagggcg tcctcgccgg tctcggcgta gtagttcttg cggatcccga ccggggcgaa
      961 gccgaactgg tggtagagac cctgggcggc gtggttggcc acccggacct cgagcgtgca
     1021 ggcggtcgcg ccccggcgcc gggcctcggt gaccagcgcg aacagcagcc gggaggcgac
     1081 cttgcggcgg tgggcggcgg ggtcgacggc gatgttggtc acgtgcgcct cgtccccgaa
     1141 caccatcagc ccggcatacc cgacgacctg ccggcgggcc ttggccacga tgtagaagcg
     1201 ggtcgagcgc tgcacgatct cggacaggaa cagggtcatg gtccacggcc gcgggtacac
     1261 ctggcgctcg atgtccagca cctgggggat gtggcgccgg cgcatcggca ccagctcgat
     1321 cgccagcggc tggcgcgggt cgtcgacctg gcgccttggc gggtagatgg tggccatcac
     1381 gccgccccgc cgcttgccag ccgccgctcg acgttggggt cgatgtcggg acgccgcagg
     1441 tacaggggag tgacgtccag cgggctggtg aactcctccc ggagcatccg cgggagggcc
     1501 agctcgacca gggcggccgc cgacggcgag gactgggtca gccccccgat cagggactgc
     1561 tcgcccaggt gctcgaacgc ctcccggtac aggagggccc cgtcgccgca gagcagcagc
     1621 tcctcgcccc tggcctcgat cccggcggcg agctggtcgg ggcggagcac ctggtagtcg
     1681 gtgatccgca ccaccccacc gggggcgtgg cggtacaggg cgcagaacac ctcgccccgg
     1741 cgggcgtcga tcaccgggca gatggcccgg cggacgtggc ggtgggcgaa ggcgatcagg
     1801 tcgaggctgg gcacggccac catcggcagc ccccatgcct gggccagcgc cttggccgtg
     1861 gagaccccga cccgcaggcc ggtgaacagc cccggcccgg tgcccacggc caccccggcc
     1921 agcgcccccg ggtcgacctc ggcctgctcc agcacccagc gcacggccgg gacgagcacc
     1981 tcggcgtggc ggcgctcgtg gcggacggcc acctcggcga gggtgcccgc ctccgacccg
     2041 acggccacgc ctgtgaccgg ggtggcggtg tccatggcga ggacgagcaa cgaagacctt
     2101 tcgagcgatg ctggcggaga acgtattgtg gcacctcaac ccgcgatcgc gccgtcccga
     2161 gaacaacctt gatggcacgc gtgctgatcg tccagggaca cgcccgctcg cgggcggaca
     2221 cggccgccac cctccgggcc ga
//
LOCUS       DASSWR010000345         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1284715, whole genome shotgun sequence.
ACCESSION   DASSWR010000345 DASSWR010000000
VERSION     DASSWR010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284715"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..755
                     /locus_tag="VFO47_05720"
     CDS             276..755
                     /locus_tag="VFO47_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788227.1"
                     /translation="MAQQVLKPAQPTDRVATLAHTAALARRKVLLQRRHQEAVASSRA
                     ICHRAQLQLARQRVRRQRRQGQAVGPLAWFGVQGVIDEQVVRAVWTDGRLSCDRILGA
                     RARLLVDLGAEFRSDDPPRRYQASLQAPPVAILLTLIRACDLATVMEVDLGGNSQNT"
     gene            complement(813..>2478)
                     /locus_tag="VFO47_05725"
     CDS             complement(813..>2478)
                     /locus_tag="VFO47_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8788228.1"
                     /translation="DPEAAFQRLRRRARNTARPIAEVAGEVIAQAHAGDATSRARVAA
                     AGSARRLAELEQVAAGFAAARTPTTVARLVVDRGLRVLDAQAGLIGLTTADGQALELV
                     AWAGYPVQVVAPWRRIPLEAATPLTEVAFQLLRHQARNTARPMVEVAAGIISQARQRN
                     TPTAAQAAAARSARRLAELEQVAVGFAAARTSTTVARLLVDRGLRALDAQAGLVGLNT
                     ADGQVLELVAWAGYPVQVVTPWRRIPLEAPTPLTEVARNGGAIWLSTTEDFQARYPDV
                     PVVVEHQAHVAIGLVVEGRPAGALGISFLQARSFSEVDRRFVQALASHCAQALERVRL
                     AEQARAARTKLATAQARALTAERAALAAQANALRAREQASYLAEVSAMLASTTDVEAV
                     LEQMAWLAVPRLGDWCQIHLRQPDGTTRHHTIAHSDPADAEVLARLTDREPLDLTGAH
                     LVAHAEQPGQAPLLLDQLDQAVLGRSARDADYLELLTALNLGSAMVVPLPGRTRMLGT
                     ITFASHQPGRYTQADLTLAQNLARRTATAIEHADRAHHPNPPTPTP"
BASE COUNT          311 a          850 c          971 g          346 t
ORIGIN      
        1 tggcgatcat cggggccatg gtgcgccccg caccaacctt ccgccacgcc aggctccatc
       61 ccccattgga agtcggacgg gtcggccttc acggctcgga ggcgctgcag ctcaccgcag
      121 atctcagcca gccgctggcg cagcggattt ctggtgccgg gaggacaacc ggtcggtggt
      181 ggccggctgc ccgccgggct tcccaccacc agcgtagccc cgtaggctag gcctgccgtg
      241 ggcaggtgca ggaccgatga tggctggtgg tggtgatggc acagcaggtc ctgaagccgg
      301 cccagcccac cgaccgggtc gcgaccttgg cccatacggc cgccttggct cgccgcaagg
      361 tgctgctgca gcggcgtcac caggaagcgg tagccagctc ccgcgccatc tgccaccggg
      421 cccagctcca gctggcccgc cagcgggtcc ggcggcaacg ccgccagggg caggcggttg
      481 ggccgctggc ctggttcggg gtccaaggag tgatcgacga gcaggtcgtt cgggcggtct
      541 ggaccgatgg gcggctgagc tgtgaccgga tactgggtgc gcgggctcgg ctgctggtgg
      601 atctgggcgc cgagttccgc agcgacgatc cgccacgtcg ctaccaggcc agcttgcagg
      661 ccccgccggt ggcgatcctg ctgacgctga tccgagcgtg tgacctggcc acggtgatgg
      721 aggtcgacct cggcgggaac tcgcagaaca cctgacaatc aggcacctgc cgcgcgcccg
      781 cagcgtggat cgccagtttc atgtgggtgc ggctaggggg tcggcgtggg gggattggga
      841 tggtgggctc ggtcggcgtg ctcgatggcg gtggcggtcc gccgggcgag gttctgggca
      901 agggtcaggt cggcctgggt gtagcggcct ggctggtggc tggcgaaggt gatggtgccg
      961 agcatccgcg tccggccggg aagcggcacg accatcgccg acccgaggtt gagcgcggtg
     1021 agcagctcca ggtagtcggc gtcccgggca ctccggccca ggacggcctg gtcgagttgg
     1081 tccagcagca gcggcgcctg gccgggctgc tcggcgtggg ccaccaagtg ggcgccggtt
     1141 aggtcgagcg gttcccggtc ggtcagccgg gccaggacct cagcgtcggc ggggtcgctg
     1201 tgagcgatgg tgtggtgacg ggtcgtgccg tcgggttgcc ggaggtggat ctggcaccag
     1261 tcgccgaggc ggggcacggc cagccaggcc atctgctcca gcaccgcttc gacgtcagtg
     1321 gtgctggcca gcatggcgct gacctcggcc aggtagctgg cctgctcgcg ggcccgcagg
     1381 gcgttggcct gggcggccag ggcggcgcgt tcggcggtga gggcccgggc ctgggcggtg
     1441 gccagcttgg tccgggcggc ccgggcctgc tcagctaggc ggacccgttc cagggcctgg
     1501 gcgcaatggc tggccaacgc ctggacgaag cgacgatcga cctcgctgaa tgagcgggcc
     1561 tggaggaagc tgattccgag ggcgccggcc gggcggccct ccacgaccag cccaatggcg
     1621 acgtgggcct ggtgctcgac gaccacgggc acatcggggt accgggcctg gaaatcctcg
     1681 gtggtcgaca gccagatggc cccgccgttc cgggccacct cggtcagcgg cgtcggcgcc
     1741 tccaggggga tgcgccgcca gggagtgacc acctggaccg gatagccggc ccaggcgacc
     1801 aactccagca cctgcccgtc ggcggtgttg agcccgacca ggccggcctg ggcgtccagg
     1861 gcccgcagcc cccggtcgac cagcagccgg gccaccgtgg tggaggtccg ggcggccgcg
     1921 aacccgacgg cgacctgttc cagctcggcc agccggcggg ccgatcgcgc cgccgcagcc
     1981 tgggccgcgg ttggcgtgtt gcgctggcgg gcctggctga tgatcccagc cgcgacctcg
     2041 accatgggtc gcgccgtgtt cctggcctgg tggcggagca gctggaaggc gacctcggtc
     2101 agcggcgtcg ccgcctccag ggggatgcgc cgccaggggg cgaccacctg gaccgggtag
     2161 ccggcccagg caaccaactc cagcgcctgc ccgtcggcgg tggtgagccc gatcaggccg
     2221 gcctgggcgt ccaggacccg cagcccccgg tcgaccacca gccgagccac cgtggtcggg
     2281 gtccgggcgg ccgcgaaccc ggccgcgacc tgttccagct cggccagccg acgggccgat
     2341 cccgccgccg cgacccgtgc ccggctcgtg gcgtcgccgg cgtgggcttg ggcgatcacc
     2401 tcgccagcga cctcggcgat cggccgggcg gtgttgcggg cccggcggcg cagccgctgg
     2461 aaggcggcct cagggtcc
//
LOCUS       DASSWR010000346         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_267453,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000346 DASSWR010000000
VERSION     DASSWR010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267453"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..769
                     /locus_tag="VFO47_05730"
     CDS             146..769
                     /locus_tag="VFO47_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET8788229.1"
                     /translation="MPRTPARSPARPDPNVLPDPLRLPWRPPVTPAAGDDPRAEPGWA
                     AGLGEASPADRRLIEAQIGRGARGSVAVAARCRYGLPLVVRTAPLLPDGTPFPTLYWL
                     ACPAARVAVGRLEAAGWNARLSERVAAEPELAEAHAAAHASYLAQRDAMAPLPGDPGV
                     GGLPGRVKCLHALYAHHAATGADPVGRIVSQAVDPIDCPGPCVDPGA"
     gene            773..1702
                     /locus_tag="VFO47_05735"
     CDS             773..1702
                     /locus_tag="VFO47_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET8788230.1"
                     /translation="MRLAALDVGTNSTRLLVADVAGGRIVAEQAREMVITRLGKGVDR
                     TGRFDPAALARTLEVLAGYAATCRRLGVERRRLVATSATRDAADRQAFLDGVRDLLGV
                     DAEVLTGQAEAAATYRGATADLPGDQPTLVVDIGGGSTELISGHGAAADAMVSLDIGC
                     VRLFERHLHRDPPGPEEVEALRADAAAHLRRVPEVLDPAAASRVVGVAGTVTTVTAIA
                     LGLGSYDPRRIHGAAVDTSEVAAVAGKLAAMTVAERAALPVMARGREDVIVAGALLLD
                     ELVRMFHLRNVIASEADILDGVLLGLAEGPHGG"
     gene            1692..2627
                     /locus_tag="VFO47_05740"
     CDS             1692..2627
                     /locus_tag="VFO47_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8788231.1"
                     /translation="MAAESQAAPAQADDQVPRYRVTAVERMTRIPASTLRSWERRYGW
                     PRPFRAASGQRLYSDHDVAVIRFLDRRRIEGMSMSQASALLRSAPAVEDHDPRRLFDR
                     LVAALRAFDEAAAERAFAAAEALLGTEGVATGVVPAAIAAAAEPGHGPGEGGDGTPVE
                     VEHFASNFLRRQVLRLLDGQPPAMGRPVLVGCGPDEQHELGALLLTLLLRARGHRVVY
                     LGARVPGPAMERAAAALGPAGVVVSLTMPESLERAVAWAHDNRRWPRPAPSSWWAGPA
                     VAAAPDLAARLPGDLLDGTVDAGAAAVAATLARRR"
BASE COUNT          273 a         1117 c          966 g          294 t
ORIGIN      
        1 gccgactggc agcccgaggc gagccggccc cgccgcgccg ccaaggaccc cgagttcgag
       61 cagaagctca agcggttcat gcgctccagc gaggaacggc tggtcgacgt caagcgccag
      121 cgcgagggcc ggcgcgggta actcggtgcc acgcaccccg gcccggtctc cggcccggcc
      181 cgaccccaac gtcctccccg atcccctgcg cctcccctgg cgcccccccg tgacgcccgc
      241 ggccggcgat gacccccgcg ccgagcccgg ctgggcggcc gggctcggcg aggccagccc
      301 ggccgaccgg cggctgatcg aggcccagat cgggcggggc gcccgcgggt cggtggccgt
      361 ggccgcccgc tgccgctacg gcctgcccct ggtggtccgg accgcgcccc tgctgcccga
      421 cggcaccccc ttcccgaccc tctactggct ggcctgcccg gcggccaggg tggccgtcgg
      481 ccggctcgag gcggccggct ggaacgcccg gctcagcgag cgggtcgccg ccgagccgga
      541 gctggccgag gcccacgccg ccgcccacgc cagctacctg gcccaacgcg acgccatggc
      601 ccccctcccc ggcgaccccg gcgtcggcgg cctccccggc cgggtcaagt gcctgcacgc
      661 cctctacgcc caccacgccg ccaccggcgc cgacccggtt ggccggatcg tcagccaggc
      721 cgtcgacccg atcgactgcc ccggcccctg cgtcgacccc ggggcctgag ccgtgcggct
      781 ggccgccctc gacgtcggca ccaactcgac ccggctgctg gtggccgacg tcgccggcgg
      841 gcggatcgtg gccgagcagg cccgcgagat ggtcatcacc cgcctcggca agggggtcga
      901 ccggaccggg cgcttcgacc cggcggcgct ggcccggacc ctggaggtgc tggccggcta
      961 cgccgccacc tgccggcgcc ttggcgtgga gcgccggcgg ctggtggcga ccagcgccac
     1021 ccgggacgcc gccgaccgcc aggcgttcct cgacggcgtc cgcgacctgc tcggcgtgga
     1081 cgccgaggtg ctcaccggcc aggccgaggc cgccgccacc taccgcggcg ccaccgccga
     1141 cctccccggc gaccagccga cgctggtggt ggacatcggc ggcgggtcca ccgagctgat
     1201 ctccggccac ggcgccgccg ccgacgccat ggtctccctc gacatcggct gcgtgcggct
     1261 gttcgagcgc cacctccacc gcgacccgcc gggccccgag gaggtggagg cgctccgggc
     1321 ggacgcggcc gcccacctgc gccgggtccc cgaggtgctc gacccggcgg ccgcctcccg
     1381 ggtcgtcggg gtcgcgggca cggtcaccac cgtcaccgcg atcgccctcg gcctcggcag
     1441 ctacgacccg cgccgcatcc acggggcggc cgttgacacg tccgaggtcg ccgccgtcgc
     1501 cggcaagctg gccgcgatga cggtggccga gcgggcggcg ctgccggtga tggccagggg
     1561 ccgggaggac gtgatcgtcg ccggggcgtt gctgcttgac gagctcgtcc gcatgttcca
     1621 ccttcggaac gtcatcgcca gcgaggccga catcctcgac ggtgtgctgc tcgggttggc
     1681 ggaaggaccc catggcggct gagagccagg cggcgccggc gcaggccgac gaccaggtgc
     1741 cccggtaccg ggtcaccgcc gtcgagcgga tgacccgcat cccggcgagc accctgcggt
     1801 cctgggagcg gcgctacggc tggccgcggc cgttccgggc cgcctcgggc cagcggctct
     1861 actccgacca cgacgtggcc gtgatccgct tcctcgaccg gcgccggatc gagggcatgt
     1921 cgatgtcgca ggccagcgcc ctgctccggt cggccccggc ggtcgaggac cacgacccgc
     1981 ggcggctgtt tgaccgcctg gtcgccgccc tgcgggcctt cgacgaggcc gccgccgagc
     2041 gggccttcgc cgccgccgag gccctcctcg gcaccgaggg cgtggccacc ggcgtggtcc
     2101 cggcggccat cgccgccgcc gccgagcccg gccacggacc gggcgagggc ggcgacggca
     2161 cgccggtcga ggtcgagcac ttcgcctcca acttcctgcg ccgccaggtc ctgcgcctgc
     2221 tcgacggcca gccgccggcc atgggccggc cggtgctggt cggctgcggc cccgacgagc
     2281 agcacgagct gggcgccctg ctgctgaccc tgctgctccg ggcccgcggc caccgggtcg
     2341 tctacctggg cgcccgcgtc cccgggccgg ccatggagcg ggccgccgcc gccctcggcc
     2401 cggccggggt ggtggtcagc ctgaccatgc ccgagtcgct ggagcgggcc gtcgcctggg
     2461 cccacgacaa ccgccgctgg ccccgccccg ccccgtcctc ctggtgggcc ggcccggccg
     2521 tcgccgccgc ccccgacctg gccgcccgcc tccccggcga cctcctcgac ggcaccgtcg
     2581 acgccggggc ggcggctgtc gccgccaccc tggcccgccg ccgctgacct cagccggagg
     2641 ccgcggccag
//
LOCUS       DASSWR010000347         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1084843, whole genome shotgun sequence.
ACCESSION   DASSWR010000347 DASSWR010000000
VERSION     DASSWR010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084843"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFO47_05745"
     CDS             complement(<1..766)
                     /locus_tag="VFO47_05745"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8788232.1"
                     /translation="MPPQQSILITGATSGIGRDAALRLVRDGHLVLAGGRRPDALAEL
                     ARTAGGRLEPVVLDVTDPVLVDTARALVERRTGGRGLDVLVNNAGYALPGPLEALAER
                     DLRELFDTNVFGLLAVTRAFLPAMRERGQGRVVNVGSIMGRVAMPLLGAYNASKRAVA
                     AVTDALRMELAPFGVTVVLVEPGAVRTGFASRALAGLAPYRDPGSPYAAALAGTDAAW
                     ARVYRLAPGPASAGRVVARAATATRPAPRYVVPARNR"
     gene            782..>2357
                     /locus_tag="VFO47_05750"
     CDS             782..>2357
                     /locus_tag="VFO47_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8788233.1"
                     /translation="MTEPRWKQRYRAPSLSLPRWAPDEPDHLVYVSNLSGSWQVHAWD
                     RAAGTHRQVSDHPTGVVAAGLTPDGARVVWFQDSKGDEVGRWLQQPFEGGETAPLVAD
                     VEPAWSAGLALGPKGLAVVGVSSRDGFSVRVGEVGGGSEELYRHDEMVDVAGVSRDGR
                     LLVVHHAEHGDNLHPALRAFDLDAGAPLADLWDGEGFGLFVASAGFSPVAGDGRLAVL
                     ADRTGSLRPAIWDPAGDQRTDLELDLPGEVDVADWWPGGGALLLIHTYMGRDELHRLD
                     LRSGALERLDHPAGSIQRAGVRPDGTVWYRHSSGAAAPEVRAVGAEEAVLRPPGPRAP
                     SGVAYESWTFPNPQGDQVHGFLAVPPGPGPHPTVLIVHGGPTHHDSDSWDPEVQAFVD
                     HGYAVGLVNYRGSTGYGKAWQDELEGDPGRPEIEDVAAGRDDLVARGLADPGQVVISG
                     ASWGGYVTLQAIGTVPDGWRAAMAVVPVADYLAAYADESEALQAFDRSLFGGGPEDKH
                     DLYVERSPITYVDKVKT"
BASE COUNT          283 a          906 c          898 g          270 t
ORIGIN      
        1 ggcggttgcg ggccgggacc acgtagcggg gggcggggcg ggtggcggtg gcggcgcggg
       61 ccaccacgcg gccggccgag gctgggccgg gggcgaggcg gtagacccgg gcccaggcgg
      121 cgtcggtgcc ggccagggcg gcggcgtacg gcgagccggg gtcgcggtag ggggcgaggc
      181 cggccagggc ccgggacgcg aacccggtcc ggaccgcgcc cggctcgacc aggaccacgg
      241 tgacgccgaa gggggccagc tccatgcgca gggcgtcggt gacggcggcc acggcgcgct
      301 tgctggcgtt gtaggcgccg agcaggggca tggcgacccg gcccatgatg ctgccgacgt
      361 tgacgacccg gccctggccc cgctcgcgca tcgccggcag gaacgccctg gtcaccgcca
      421 gcaggccgaa cacgttggtg tcgaacagct cgcgcaggtc ccgctcggcc agcgcctcca
      481 gcggccccgg gagggcgtag ccggcgttgt tgaccagcac gtccagcccc cggccgccgg
      541 tgcgccgctc gaccagcgcc cgggccgtat ccaccaacac cgggtcggtc acgtccagca
      601 ccaccggctc cagccggccc ccggccgtcc gggccagctc ggccaaggcg tcgggccggc
      661 gcccgccggc caggaccagg tgcccgtccc ggaccagccg cagggccgcg tcccggccga
      721 tgcccgaggt cgcgcccgtg atcaggatgc tctgttgtgg cggcatgcgt acgatgctcg
      781 catgaccgag cctcgctgga agcagcgcta ccgcgccccc tcgctgagtc tgccgaggtg
      841 ggcccctgac gagcccgacc acctggtcta cgtctccaac ctctccggtt cctggcaggt
      901 ccacgcctgg gaccgggccg ccggcaccca ccgccaggtg tcggaccacc cgaccggcgt
      961 cgtcgccgcc ggcctgaccc ccgacggagc cagggtcgtc tggttccagg actccaaggg
     1021 cgacgaggtc ggccgctggc tccagcagcc gttcgagggg ggcgagaccg cgccgctggt
     1081 ggccgacgtc gagccggcct ggagcgccgg gctcgccctc gggcccaagg gcctggccgt
     1141 ggtcggggtg tcgagccggg acggcttctc ggtccgggtc ggcgaggtcg gcgggggcag
     1201 cgaggagctg taccgccacg acgagatggt cgacgtcgcc ggggtcagcc gcgacggccg
     1261 cctgctggtc gtgcaccacg ccgagcacgg cgacaacctc cacccggcgc tgcgcgcctt
     1321 cgacctggac gccggggccc cgctggccga cctgtgggac ggcgagggct tcggcctgtt
     1381 cgtggcctcg gccggcttct cgccggtggc cggcgacggg cgcctggccg tgctcgccga
     1441 ccggacgggt tcgctgcggc cggccatctg ggacccggcc ggcgaccagc ggaccgacct
     1501 ggagctcgac ctgcccggcg aggtcgacgt ggccgactgg tggcccggcg gcggggccct
     1561 gctgctgatc cacacctaca tgggccgcga cgagctgcac cgcctggacc tgcgcagcgg
     1621 cgccctggaa cggctcgacc acccggccgg gtccatccag cgggccgggg tgcggcccga
     1681 cgggacggtc tggtaccgcc actcctcggg ggcggccgcg cccgaggtcc gggccgtcgg
     1741 cgccgaggag gcggtgctgc ggccgcccgg gccgcgcgcc ccctccggcg tcgcctacga
     1801 gagctggacg ttccccaacc cccagggcga ccaggtccac gggttcctgg ccgtgccgcc
     1861 gggcccgggc ccacacccga cggtgctgat cgtccacggc ggccccaccc accacgactc
     1921 cgactcctgg gaccccgagg tccaggcctt cgtcgaccac ggctacgcgg tcgggctggt
     1981 caactaccgc ggctccaccg gctacggcaa ggcctggcag gacgagctcg agggcgaccc
     2041 tgggcggccc gagatcgagg acgtggccgc cggccgcgac gacctggtcg cgcgcggcct
     2101 ggccgacccc ggccaggtgg tcatcagcgg cgcctcctgg ggcgggtacg tgaccctgca
     2161 ggccatcggc accgtccccg acggctggcg ggcggccatg gccgtggtcc cggtggccga
     2221 ctacctggcc gcctacgccg acgagtccga ggccctccag gcgttcgacc gcagcctgtt
     2281 cggcggcggc cccgaggaca agcacgacct gtacgtcgag cgctcgccca tcacctacgt
     2341 cgacaaggtc aagaccc
//
LOCUS       DASSWR010000348         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_804489,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000348 DASSWR010000000
VERSION     DASSWR010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804489"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFO47_05755"
     CDS             <1..986
                     /locus_tag="VFO47_05755"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET8788234.1"
                     /translation="AAWQAGRTGYPLVDAGMRQLTGGRRSPTGGSGTGAGGFGVEGAV
                     EVTPPDPEAALERLRWFVERRIDRYARARNYMATDGTSRLSPYLRFGLLSPRQVAAAV
                     GRGPRAAEHPRRAGDRETFVNELLWRDFYGYVLYHQPESAWAHMRPEFARFAFEDDPD
                     GLAAWQAGRTGYPLVDAGMRQLTGQGWVHNRARMVVASFLTKHLLVDWREGARFFLQH
                     LMDGDLPANNGGWQWVAGTGTDAAPYYRIFNPVTQAEKFDPDGDYIRRWVPELAKLQP
                     PAIFAPWQASPADLEAGGVRLGTDYPHPIVDHPTARDRALARYQEASPRRT"
     gene            complement(1040..1639)
                     /gene="recR"
                     /locus_tag="VFO47_05760"
     CDS             complement(1040..1639)
                     /gene="recR"
                     /locus_tag="VFO47_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET8788235.1"
                     /translation="MYEGPVQDLIDELGRLPGIGPKSAQRLAFHILKVEPADAERLAR
                     AITEVKARVHFCRVCWNVAEGDLCRICKDPRRDPGLVCVVEEPRDLVAIERTQEFRGR
                     YHVLGGAISPIEGVGPDDLRIRELLGRLEPDSIREVILATNPNIEGEATAMYLARLLA
                     PLGVRVTRIASGLPVGGDLEYADEITLGRAFAGRREMPV"
     gene            complement(1644..1973)
                     /locus_tag="VFO47_05765"
     CDS             complement(1644..1973)
                     /locus_tag="VFO47_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET8788236.1"
                     /translation="MPRGGFNPNQMLKQLQKMQREMEDTQAQLAEEVVEGTAGGGVVT
                     ARVKGDQTVESVSIDPDALDPDDVEMLGDLVTAAVNEGLRKAKDLAAERLGGITQGMG
                     LPPGLGL"
     gene            complement(1982..2533)
                     /locus_tag="VFO47_05770"
     CDS             complement(1982..2533)
                     /locus_tag="VFO47_05770"
                     /inference="COORDINATES: protein motif:HMM:NF044669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788237.1"
                     /translation="MAEPPPEEAGWGPAEAPAQVPVDLDLVRRSWPLVLERVQAASRL
                     TASFLGHGRPVALEGRRLVIELPKDRPFEAEALAGRSRQVDGVLEAVLGGGLEIRVVV
                     GEHAGPEEPDPPPAPGPAPASDQVVEFEVDPANDEGRVLDADADARAVADWAAKELGG
                     KVIEERPNDSSAKGGARGARKGR"
     gene            2553..>2736
                     /locus_tag="VFO47_05775"
     CDS             2553..>2736
                     /locus_tag="VFO47_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PGRS family protein"
                     /protein_id="HET8788238.1"
                     /translation="MGLALAGGDLAGAAAAGAEGEDGVEAREAEGRAGATAAGDGAFR
                     AAGSAAAGAGAAGTAPS"
BASE COUNT          322 a         1060 c         1000 g          354 t
ORIGIN      
        1 tggccgcctg gcaggccggc cgcaccggct acccgctggt cgacgccggc atgcgccagc
       61 tcaccggtgg gcgacgctcc cccaccgggg gctccggcac cggagcgggc gggttcgggg
      121 tggagggggc ggtggaggtc accccgcccg acccggaggc ggcgctggag cggctgcgct
      181 ggttcgtgga gcggcggatc gaccgctacg cgagggcgcg gaactacatg gcgaccgacg
      241 ggacctcgcg cctcagcccg taccttcgct tcgggctcct ctcgccccgc caggtcgcgg
      301 ccgccgtcgg ccgcgggcca agggcggccg agcacccccg ccgggccggg gaccgcgaga
      361 ccttcgttaa cgagctgctg tggcgcgact tctacggcta cgtcctctac caccagccgg
      421 agagcgcctg ggcgcacatg cgccccgagt tcgcccgctt cgcgttcgag gacgacccgg
      481 acgggctggc cgcctggcag gccggccgca ccggctaccc gctggtcgac gccggcatgc
      541 gccagctcac cggccagggc tgggtccaca accgggcccg catggtggtg gcctcgttcc
      601 tgaccaagca cctgctcgtc gactggcgcg agggcgcccg cttcttcctc cagcacctca
      661 tggacggcga cctgccggcc aacaacggcg gctggcagtg ggtcgccggc accggcaccg
      721 acgccgcccc ctactaccgg atcttcaacc ccgtcaccca ggcggagaag ttcgaccccg
      781 acggcgacta catccgccgc tgggtccccg agctcgccaa gctccagccc ccggccatct
      841 tcgccccctg gcaggccagc cccgccgacc tcgaagccgg cggggtccgc ctcggcaccg
      901 actaccccca ccccatcgtc gaccacccca ccgcccgcga ccgcgccctg gcccgatacc
      961 aggaggcctc gccccgccgt acctgacctt ggcagcccat ccagctgaag agcttgccga
     1021 ggcgtgaacg ccaggaggcc tacaccggca tctcgcggcg gccggcgaag gcgcggccca
     1081 gggtgatctc gtcggcgtat tcgaggtcgc cgccgacggg gaggccgctg gcgatgcggg
     1141 tgacccggac gccgaggggg gcgaggaggc gggccaggta catggcggtg gcctcgccct
     1201 cgatgttggg gttggtggcc aggatgacct cacggatcga gtcgggctcg aggcggccca
     1261 gcagctcgcg gatgcggagg tcgtcggggc cgaccccctc gatcgggctg atggccccgc
     1321 cgagaacgtg gtagcggccg cggaactcct gggtgcgctc gatcgcgacc aggtcgcggg
     1381 gctcctcgac gacgcagacc aggccggggt cgcggcgcgg gtccttgcag atgcggcaga
     1441 ggtcgccctc ggccacgttc cagcagaccc ggcagaagtg gacccgcgcc ttgacctcgg
     1501 tgatggccct ggccaggcgc tcggcgtcgg ccggctcgac cttgaggatg tggaaggcca
     1561 gccgctgggc gctcttggga ccgatgcccg gcagccgccc gagctcgtcg atcaggtcct
     1621 ggacggggcc ttcgtacatg ggctcagagc cccaggccgg gtggcagccc catgccctgg
     1681 gtgatgccgc cgaggcgctc ggcggcgagg tccttggcct tgcgcaggcc ctcgttgacg
     1741 gcggcggtga ccaggtcgcc gagcatctcg acgtcgtcgg ggtcgagcgc gtccgggtcg
     1801 atcgagaccg actccaccgt ctggtcgccc ttgaccctgg ccgtgaccac gccgccgccg
     1861 gccgtcccct cgacgacctc ctcggccagc tgggcctggg tgtcctccat ctcgcgctgc
     1921 atcttctgca gctgcttgag catctggttg gggttgaacc cacccctggg catgcccatt
     1981 gctaccgtcc cttcctcgcg ccccgggcgc cgcccttggc ggacgagtcg ttcggacgct
     2041 cctcgatgac cttgccgccg agctccttgg cggcccagtc tgccaccgcg cgggcgtcgg
     2101 catcggcgtc gagcacccgg ccctcgtcgt tggccgggtc gacctcgaac tcgaccacct
     2161 ggtcggacgc gggcgccggg cctggagcgg gcggtgggtc gggctcctcg gggccggcgt
     2221 gctcgccgac cacgacccgg atctccaggc cgccgcccag caccgcctcc aggacgccgt
     2281 ccacctgccg gctgcggccg gccagggcct cggcctcgaa gggccggtcc ttcggcagct
     2341 cgatgaccag gcggcggccc tccagggcga ccggccggcc gtggcccagg aagctggccg
     2401 tgagccggct ggccgcctgc acccgctcca gcaccagcgg ccaggaccgc cgcaccaggt
     2461 cgaggtcgac cggcacctgg gcaggcgcct ccgccggccc ccagcccgcc tcctcgggcg
     2521 gcggctcggc cacctggtcc cccgccctgg ccttgggcct ggccttggcc ggcggggact
     2581 tggccggggc ggccgccgcc ggggccgagg gcgaggacgg ggtcgaggcc cgggaggccg
     2641 agggccgggc cggggccacc gcagcgggcg acggggcctt ccgggccgcc ggctcggccg
     2701 ccgccggggc gggggcggcc gggaccgcgc cctcgc
//
LOCUS       DASSWR010000349         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1142933, whole genome shotgun sequence.
ACCESSION   DASSWR010000349 DASSWR010000000
VERSION     DASSWR010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142933"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VFO47_05780"
     CDS             complement(<1..163)
                     /locus_tag="VFO47_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET8788239.1"
                     /translation="MVEPQTLSAAYERCRLLHARHGRTYYLATLLLPRWKRRHVHALY
                     GFTRYADEIV"
     gene            complement(172..1656)
                     /gene="crtI"
                     /locus_tag="VFO47_05785"
     CDS             complement(172..1656)
                     /gene="crtI"
                     /locus_tag="VFO47_05785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET8788240.1"
                     /translation="MAGRTVVVGAGLAGLSAAIHLAAAGRDVVVVEAEAVPGGRCGTV
                     TAGGYRFDTGPSVLTMPEVVRACVGAAGEELEDRLELAPLDPIYRLTFHDGSRLDVVP
                     GAERMAAEVERLAGPEEAARYLAFRAHLGRMLDAEWDQFIDRNLDGPADLVRPLALLR
                     LARLGGFRRLHNLVATHLSDWRLRRAHTFQALYTGLSPFDALGIYAVVAHMDTVGGAW
                     FPRRGGMHALPLALAGVAEKAGAGFRYSARVERVEGGPGGVTGVVLAGGERLAATDVV
                     VTSDLPAAYEHLLPPAARDWRVRRRRLRFSPSCYLVHLGLTRQLQGQAHHTVHLGRDW
                     KATFEALTRHGGIQPDPSLLVTYPSPEDPDAAPPGHATLFVLEPTANTRAGLDWAEVG
                     PRLRERLYGRLAGLGYGDLRRDSAVELVVDPPAWEAQGLTAGTPFSLDHRFTQTGWLR
                     PGNASAGVPGLVFAGMGTVPGVGVPMVLISGRLAAERVLARRRR"
     gene            complement(1663..>2582)
                     /locus_tag="VFO47_05790"
     CDS             complement(1663..>2582)
                     /locus_tag="VFO47_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8788241.1"
                     /translation="PAFVYWGYRGAGGAPDGPAAEAAIRASCSVELLHVCALIHDDIM
                     DGSEVRRGRPAMHVGFAGLHRGRGWRGDPTAFGEGAAMLMGDLAFTWADVALAEAGLP
                     DDRLAAALRVFNRLRSELMGGQYLDLVEARRGAPDEAAVRRVLTYKSGKYTIERPLHL
                     GLALAAGPPALAAGYSAYGLPLGEAFQLRDDILGVFGSPEVTGKPAGDDLREGKETYL
                     VMRARRLAGRAGRALLEGALGNAKLSEDEVAELRRLIAGCGAVDATEARIGELLAEAK
                     AALAATDGIDEAARGTLAALADHVTDRSA"
BASE COUNT          330 a          962 c         1017 g          273 t
ORIGIN      
        1 cgacgatctc gtcggcgtag cgggtgaagc cgtacagggc atggacgtgg cggcgcttcc
       61 agcgggggag caacaaggtg gccaggtagt aggtgcggcc gtggcgggca tggagcagcc
      121 ggcaccgctc ataggccgcc gacaacgtct gcggctccac catggccagc ctcatcgccg
      181 cctccgggcc agcacccgct cggcggccag gcggccggag atcagcacca tcggcacccc
      241 gaccccgggc acggtgccca tcccggcgaa cacaaggccg ggcacgcccg ccgaggcgtt
      301 cccgggccgc agccagccgg tctgggtgaa gcggtggtcg agcgagaacg gcgtccccgc
      361 ggtcaggccc tgggcctccc aggcgggagg gtcgacgacc agctcgacgg cgctgtcgcg
      421 gcgcaggtcg ccgtagccga gcccggccag ccgcccgtac agccgctcgc gcaggcgcgg
      481 cccgacctcg gcccagtcca gcccggcccg ggtgttggcg gtcggctcga gcacgaacag
      541 ggtggcgtgg ccgggcgggg cggcatcggg gtcctcgggc gaggggtagg tgacgagcag
      601 gctcgggtcg ggctggatgc cgccgtggcg ggtcagggcc tcgaaggtcg ccttccagtc
      661 ccggcccagg tggacggtgt ggtgggcctg gccctggagc tggcgggtca gccccaggtg
      721 gaccaggtag caggagggcg agaaccgcag ccggcgccgc cgcacccgcc agtcgcgggc
      781 cgccgggggc agcaggtgct cgtaggcggc cggcaggtcg ctggtcacga ccacgtcggt
      841 ggcggccagg cgctcgccgc cggccagcac caccccggtg accccgccgg gcccgccctc
      901 gacccgctcg acccgggccg agtaccggaa gcccgccccg gccttctcgg ccaccccggc
      961 cagggccagg gggagggcgt gcatgccgcc ccggcggggg aaccaggcgc cgccgacggt
     1021 gtccatgtgg gcgaccacgg cgtagatgcc gagggcgtcg aacggcgaca ggccggtgta
     1081 gagggcctgg aaggtgtggg cccggcgcag ccgccagtcg ctcaggtggg tcgcgaccag
     1141 gttgtgcagc cgccggaacc cgccaaggcg ggccagccgc aggagggcca ggggccggac
     1201 caggtcggcc gggccgtcga ggttgcggtc gatgaactga tcccactcgg cgtccagcat
     1261 ccggcccaga tgcgcccgga aggccaggta gcgggcggcc tcctcgggcc cggcgagccg
     1321 ctcgacctcg gccgccatgc gctcggcccc cgggaccacg tccaggcggc tgccgtcgtg
     1381 gaaggtaagc cggtagatcg ggtcgagggg ggccagctcc agccggtcct ccagctcctc
     1441 gccggcggcg ccaacgcacg cccgcaccac ctcgggcatg gtcagcaccg aggggccggt
     1501 gtcgaagcgg tagccgccgg ccgtgaccgt gccgcagcgg ccgcccggga cggcctcggc
     1561 ctcgaccacg accacgtcgc ggccggccgc cgccaggtgg atggcggccg acaggccggc
     1621 caggccggcc ccgacgacga ctgtccgccc cgccatatcc ggctaggcgc tccggtcggt
     1681 gacgtggtcg gccagcgccg ccagggtgcc ccgggccgcc tcgtcgatcc cgtcggtcgc
     1741 ggccagcgcc gccttggcct cggccaggag ctcgccgatg cgggcctcgg tggcgtccac
     1801 ggccccgcag ccggcgatca gccggcgcag ctcagcgacc tcgtcctcgg acagcttggc
     1861 gttgccgagg gccccctcca gcagggcccg gccggcccgg ccggcgagcc ggcgggcccg
     1921 catgaccagg taggtctcct tgccctcgcg caggtcgtcc ccggccggct tgccggtcac
     1981 ctccggggac ccgaacaccc cgaggatgtc gtcccggagc tggaaggcct cgcccagggg
     2041 cagcccgtag gccgagtagc cggcggccag ggcgggcggg ccggcggcca gggccaggcc
     2101 caggtgcagc ggccgctcga tggtgtactt gcccgacttg taggtcagga cccggcggac
     2161 ggcggcctcg tcgggggcgc cccggcgggc ctcgaccagg tccaggtact ggccgcccat
     2221 cagctccgag cgcagccggt tgaagacccg cagggcggcg gccaggcggt cgtcgggcag
     2281 gccggcctcg gccagggcca cgtcggccca ggtgaaggcc aggtcgccca tgagcatggc
     2341 cgcgccctcg ccgaaggcgg tcgggtcgcc ccgccagccg cggccgcggt ggaggccggc
     2401 gaagccgacg tgcatggccg ggcggccgcg ccggacctcg gagccgtcca tgatgtcgtc
     2461 gtggatgagg gcgcagacgt gcagcagctc gaccgagcag gaggcccgga ttgccgcctc
     2521 ggcggcaggg ccgtcggggg cgcccccggc ccctcggtag ccccagtaga cgaaggccgg
     2581 cc
//
LOCUS       DASSWR010000350         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_126531,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000350 DASSWR010000000
VERSION     DASSWR010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126531"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFO47_05795"
     CDS             complement(<1..394)
                     /locus_tag="VFO47_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET8788242.1"
                     /translation="MAQHDARIIAVVGATGLQGGAVTRRLLQDHWPVRALTRNPNAKK
                     ARALAHLGAEVVKAEMSDPASLERAFDGVHGVFSVQNHHLSGYEGEVRQGKHVADAAK
                     RTGVAHIVYSAAGIGVEGTGVGSWETKVA"
     gene            complement(689..1141)
                     /locus_tag="VFO47_05800"
     CDS             complement(689..1141)
                     /locus_tag="VFO47_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8788243.1"
                     /translation="MVEIRFEVDTALSPQQVRAAATDFSERRPQIWSGIDPKRFTVHA
                     LGDSTAEVTEGGREFGGIWAREHYDWSDPDRVVARVLDSNIFGPGSRWELRIRPTPAG
                     GSRVEWASLRQPSSLKGRLVVLILRVAGRQHLRNYLHKTLSKLEHQTA"
     gene            1644..2913
                     /locus_tag="VFO47_05805"
                     /pseudo
     CDS             1644..2913
                     /locus_tag="VFO47_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          465 a         1016 c          999 g          481 t
ORIGIN      
        1 ccgcgacctt ggtctcccag gacccaacgc cggtgccctc gacgccgatg ccggccgcgc
       61 tgtagacgat atgggcaaca cccgtccgct tggccgcatc ggcgacgtgc tttccctgcc
      121 gcacctcccc ctcatagccg ctgaggtggt ggttctgcac gctgaagacg ccatggacgc
      181 catcgaaggc acgctccaga gacgcaggat ccgacatctc ggccttgacg acctcggcgc
      241 caaggtgggc gagggcgcgg gccttctttg cattcgggtt cctggtcagg gcccgaaccg
      301 gccaatggtc ctgcagcagg cggcgggtca ccgccccacc ctgcaggccg gtcgccccca
      361 ccaccgcgat gatccgagcg tcgtgctgcg ccatcgctgt cgccttcccc aaaccaagac
      421 aagcttgaga ggtgagatcc tagcgaagtt cggcatgcaa gtcttctgct tgccgcctcg
      481 gggtcgagca gcccgccttg actggcgttg tcgagcgcga ccggcgtcgc gttgacggtg
      541 ttcgcgggcc agccgatagc cgctgtgatc agcatggcgg tgaaacgatg ggagagactt
      601 cggtcagtgc agccaccatg ttcggctcgc ccatcgccgg ttcctggcgg ggacgacggt
      661 catcgcatcc ggctgccgtg ctgttcgctc acgcggtctg gtgctcgagc ttggataggg
      721 tcttgtgcag atagttacgg aggtgctggc ggccggcgac cctcaggatc aggaccacga
      781 ggcgtccttt gaggctgctg ggctggcgga gcgaggccca ctccacgcgg ctcccgcccg
      841 ccggtgtcgg tcggatccgc aactcccacc ggctgccggg accgaagatg ttggagtcca
      901 gcacccgggc gaccaccctg tccgggtcgg accagtcgta gtgctctcga gcccagatgc
      961 ccccgaactc gcggccgccc tcggtgacct cggcggtact gtcgcccagg gcgtggacgg
     1021 tgaagcgctt ggggtcgatc ccggaccaga tctggggccg ccgctcggag aagtcggtgg
     1081 ctgcggctct gacttgctgg ggggacaggg cagtgtccac ctcgaaccga atctcgacca
     1141 tggtcgcctc ctttcaggcg agccaaggat gtgccgacgc cagacggggg ggcatcggta
     1201 ggttcgccca tccttggctc agccgcgcag caggccatgt tccataccgc cgggcgccag
     1261 gcatcgaccg ctgaagcgta gtgcggccag ttctacggcg accaagacca gccacgcggc
     1321 tgacagtaaa ttcaccccat ccttgtagga cttacggagt cccacgacca gcgcaaaggc
     1381 cggatagcac agcgctcagc cgtcgatctc tgataagccg cggtgccacc ctatgggagg
     1441 cgcccgttcc cgaatgaaaa tcggggtggt tgcccgctga cgccggatct ccttggtcgt
     1501 cggaggccgt tgataagccg ggtgctggga gccgcgcctg ggcccttcac tgtcgcctcg
     1561 agcacgcgga tcagatctgc ctctcgccct gcggctcctg cgggcaacca cctttcgggt
     1621 cgtcggagcg cagggaggcc ggggtgctgg aggagacaca cgacaacgac cagatcatcc
     1681 agcgagtcgc ggccttggac gtgggcaagg ccgaactgac ctgctgtata cgggtctcgg
     1741 gccagggcag ctcgggcccg cggctgcagg aggtccggac ctaccagacg atgacccgct
     1801 cgctgctggt gctggccgac cggctggccg agctgggcgt gacccgggtg gtgatggagg
     1861 ccaccagcga ctactggaga ggggccttct acctgctgga agcccacggg tttgagacct
     1921 ggctggtgaa tgcccgtgac gtcaagcacc tcccgggacg gcccaagacc gacacgctgg
     1981 acgcggtgtg gctgtgcaag gtcgccgagc ggcagatgct gcggcccagc ttcgtccccc
     2041 cacgaccggt ccggcagctc cgtcaggtga cccgctaccg ggcggatctg gtggcggcgc
     2101 ggaccgcgga gaagcagcgg gtggagaagc tgctggagga cgcccagatc aaactgtcgg
     2161 tggtcgcctc cgacatcttc ggggtttctg ggcgggccat gcttgctgcg cttgtcgccg
     2221 gggagcgtga cccgaagcgt ctggcgcagc tggcccgtgc tcggatgcgc gccaagctgt
     2281 cgttgctgga ggaggcgttc accgggttct tcaccgacca gcacgccttc ctactgggca
     2341 agatgctggg ccgggtggac gcgctggacg gcgatctcgc cgagctcgac gccaagctcc
     2401 aggagctgat cgccccgttc accgacgcgg tcgaccggct ggatgagatc cccgggatcg
     2461 gccagctcgc cgcgcagctg ctcatcgccg agctcggggc ggacacgggc caggttcccg
     2521 accgccgggc acctggtgtc gtgggccaag ttcgccccgg gcgtcaagga gtctgccggc
     2581 aaacgcaagg gccgcgggtc gaccgggcac ggcaacccct acctggctcg tgtgctcggc
     2641 gaggtcgccg tcgccgccag caagaccgat accttcctgg gtgcacgcta ccggcggatc
     2701 gaccgccgcc gcggtgccaa gcgggccatc gtcgcggtcg gccggtccat cctggtcatc
     2761 gcctggcacc tgctgtccga cccagaagac cacttccatg acctgggcgc cggcttctac
     2821 gacacccgca tcggttctga acgcgccaag cgcaaccaca tccgccagct cgaggccctc
     2881 ggctacaagg tcaccctcga acccgccgcc tgaccggcca tcaccagacc acccactgca
     2941 ccccgatcgg atccgggctc a
//
LOCUS       DASSWR010000351         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_691824,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000351 DASSWR010000000
VERSION     DASSWR010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691824"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFO47_05810"
     CDS             <1..807
                     /locus_tag="VFO47_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate phosphoribosyl transferase"
                     /protein_id="HET8788244.1"
                     /translation="VEITGPYRLFAHLESVYLGVLARRTLVAGNVRRVVDAAGGKPVL
                     FFADRFDHWATQGGDGYAAFVGGAQGVATDAQAAWWGERGLGTIPHALIATFGGDTVA
                     AVAAFARQFPGGPLIALVDFDNDSVATSLACARAFGERLWGVRLDTSSTMVDRSLWSE
                     LGDFAPTGVNRRLVEKVRQALDAEGFRHVRVIVSGGFTAERIAAFEATGVPVDSYAVG
                     SSLLKGSADYTADVVLREGRPCAKVGRIHRPSTRLEPVDLAAHRSAGAPV"
     gene            840..1790
                     /locus_tag="VFO47_05815"
     CDS             840..1790
                     /locus_tag="VFO47_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8788245.1"
                     /translation="MQFRRLGSDGPEISVVGVGAWAIGGPWQFGWGPQDDDESIAALH
                     KAFESGVNWVDTAAVYGLGHSEEVVGQVVREHGGEVLVFTKCGRPWYGREHNEPTYDL
                     RPETIRFELEQSLKRLGTDHVDLYQFHWPDRETGTPVEDSWATMAALVEEGKVRWAGV
                     SNFDLGLLERCQRIRHVDSLQPPFSLIKRRVGQAELPWAAANGTGVICYSPLGSGLLS
                     GGFDAERARNLPAGDWRRTDPDFQPPRLEANLEMARRLEPVAERHGVPVAAVAVAWVL
                     ATEGVTGAIVGARRPSQVDGWLPAGNLRLTEADLAELAAT"
     gene            2057..>3111
                     /locus_tag="VFO47_05820"
     CDS             2057..>3111
                     /locus_tag="VFO47_05820"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8788246.1"
                     /translation="MARTYVLDTNVLIADPEAPGRFQEHDVAIPLTVVEELDKLKTRP
                     DETGASARRAIRTLERLRQQGNLSEGVELPGGGRIWVEVSLASRLDLPDALSSDSEDN
                     RILATTANLAKELAGEREVVLVSKDASLRIKAEALKLLAEEYRHERVAIEEGYLGVAT
                     CEQAGGIDAIYAARGGAILADQPLWANQFLVLRSGNQSALGQVRRSVEAGETVEVALV
                     GEPPETFGVRARSKEQQFALHLLHDPKVPLVSLSGNAGTGKTYLAVAAGLEATLEQDL
                     YDRVLVFRPVVPVGRQDLGFLPGDVDEKISPWMKAIHDTLAQLFRGSHDADRRESYTQ
                     DLVQGLLDDGTVQLEPLT"
BASE COUNT          425 a         1139 c         1133 g          414 t
ORIGIN      
        1 gtggagatca ccggccccta ccggctgttc gcccacctgg agagcgtcta cctgggcgtg
       61 ctggcccgcc ggaccctggt cgccggcaac gtgcgccggg tggtggacgc ggccgggggc
      121 aagccggtgc tgttcttcgc cgaccgcttc gaccactggg cgacccaggg cggcgacggc
      181 tacgccgcct tcgtcggcgg cgcccagggg gtggccaccg acgcccaggc cgcctggtgg
      241 ggcgagcgcg gcctgggcac catcccccac gccctgatcg ccaccttcgg cggcgacacg
      301 gtggccgcgg tggccgcctt cgcccgccag ttccccgggg ggccgctgat cgccctggtc
      361 gacttcgaca acgactcggt cgcgacctcg ctggcctgcg ccagggcgtt cggggagcgc
      421 ctgtgggggg tgcggctgga cacctcgagc accatggtcg accgctcgct gtggtcggag
      481 ctgggggact tcgcccccac cggggtcaac cggcgcctgg tcgagaaggt ccgccaggcc
      541 ctggatgccg aagggttccg ccacgtccgg gtgatcgtct cgggcggctt cacggccgag
      601 cggatcgccg ccttcgaggc caccggggtc ccggtcgact cctacgccgt cggctcctcc
      661 ctgctcaagg gcagcgccga ctacaccgcc gacgtggtcc tgcgcgaggg ccggccctgc
      721 gccaaggtcg gccgcatcca ccgcccctcg acccgcctgg agccggtcga cctggccgcc
      781 caccggagcg ccggggcccc ggtatagact gctcggctga ccaccgacga ggaggcgcca
      841 tgcagttccg gagactgggg tcggacgggc ccgagatctc ggtcgtcggg gtcggggcct
      901 gggccatcgg ggggccgtgg cagttcggct gggggccgca ggacgacgac gagtcgatcg
      961 ccgccctcca caaggcgttc gagtcggggg tgaactgggt cgacacggcc gcggtgtacg
     1021 ggctcgggca ctccgaggag gtcgtcggcc aggtcgtgcg cgagcacggc ggcgaggtgc
     1081 tggtgttcac caagtgcggc cggccctggt acggccggga gcacaacgag cccacctacg
     1141 acctgcgccc cgagacgatc cggttcgagc tggagcagag cctcaagcgc ctcggcaccg
     1201 accacgtcga cctgtaccag ttccactggc ccgaccgcga gaccggcacg ccggtcgagg
     1261 actcctgggc gaccatggcc gcgctggtgg aggagggcaa ggtccgctgg gccggggtgt
     1321 ccaacttcga cctgggcctg ctggagcgct gccagcggat ccgccacgtc gactccctgc
     1381 agccgccctt ctcgctgatc aagcgtcgcg tcggacaggc ggagcttccc tgggcggcgg
     1441 ccaacggcac cggggtcatc tgctacagcc cgctcgggtc gggcctgctc agcggcggct
     1501 tcgacgccga gcgggcccgc aacctgcccg cgggcgactg gcgccggacc gaccccgact
     1561 tccagccgcc gaggctggag gccaacctcg agatggcccg gcggctggag ccggtggccg
     1621 agcgccacgg ggtcccggtg gccgccgtgg ccgtggcctg ggtcctggcc accgaggggg
     1681 tgaccggcgc gatcgtcggc gcccgccgcc cctcccaggt cgacggctgg ctcccggccg
     1741 gcaacctgcg gctcaccgag gccgacctgg ccgagctcgc ggctacgtag cattacgggg
     1801 cagagccgga caaagagaaa ggccgcggtg gctccgcggc ccttccctgg cgtcgtcgtg
     1861 acgactgacg ctacccgccc ccacggcgct tcccgggact ccgctacggc ctgatccccc
     1921 agaacaggcc gccggagaag aagcggtccc gggccgcgcc gtccccataa tcgggggcgg
     1981 tctgtacccg cacgcggagt ctagttcgag gtaactagtc attgcaagtc cggattcgtc
     2041 aggtaccgtt ctggtgatgg ctcgcaccta cgtgctcgac accaacgtgc tcatcgccga
     2101 cccagaggcc ccgggtcgct tccaggagca tgacgtcgcc atccccctga ccgtggtcga
     2161 ggagctggac aagctcaaga cccggcccga cgagaccggg gcgtcggccc ggcgggccat
     2221 ccgcaccctg gagcgcctcc gccagcaggg caacctgtcg gagggggtgg agctgcccgg
     2281 cggcggccgc atctgggtcg aggtcagcct ggcctcccgg ctcgacctgc ccgacgccct
     2341 ctcctcggac tccgaggaca accggatcct ggccaccacg gccaacctgg ccaaggagct
     2401 ggccggggag cgcgaggtgg tgctcgtctc caaggacgcc agcctgcgca tcaaggccga
     2461 ggccctgaag ctgctggccg aggagtaccg ccacgagcgg gtggccatcg aggagggcta
     2521 cctgggcgtg gccacctgcg agcaggccgg cgggatcgac gccatctacg ccgcccgggg
     2581 cggcgcgatc ctcgccgacc agccgctctg ggccaaccag ttcctggtcc tgcgctcggg
     2641 caaccagagc gccctcggcc aggtccggcg cagcgtcgag gccggcgaga cggtcgaggt
     2701 ggccctggtc ggggagccgc cggagacctt cggggtgcgg gcccgctcca aggagcagca
     2761 gttcgccctg cacctgctcc acgaccccaa ggtgccgctg gtgtcgctgt ccgggaacgc
     2821 cggcaccggc aagacctacc tggccgtggc cgccgggctg gaggcgaccc tggagcagga
     2881 cctgtacgac cgggtgctgg tgttccgccc ggtggtgccg gtcggccgcc aggacctggg
     2941 gttcctgccc ggcgacgtcg acgagaagat ctccccctgg atgaaggcca tccacgacac
     3001 cctggcccag ctgttccggg gcagccacga cgccgaccgg cgcgagagct acacccagga
     3061 cctggtccag gggctgctcg acgacggcac ggtgcagctg gagccgctga c
//
LOCUS       DASSWR010000352         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1085164, whole genome shotgun sequence.
ACCESSION   DASSWR010000352 DASSWR010000000
VERSION     DASSWR010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085164"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFO47_05825"
     CDS             complement(<1..489)
                     /locus_tag="VFO47_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8788247.1"
                     /translation="MGRLSWRLATVAEVRPETDRVVTLALEVPEWDGHLPGQHVDVRL
                     TAEDGYQAERAYSIASAPGGERVELAVERLDDGEVSPYLAGELRQGDQLELRGPVGGY
                     FVWEPGRGGPLLLVAGGSGVVPLMAMLRHRAATGSQLPARLLYSSRTLEDVIFREELD
                     RLA"
     gene            complement(477..1094)
                     /locus_tag="VFO47_05830"
     CDS             complement(477..1094)
                     /locus_tag="VFO47_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET8788248.1"
                     /translation="MGGFVSRGFRGRRRAGTEQAAKLPPGQYAERGFPVLTAGPTPRI
                     DLAEWRLKVEGLVATPQEWTWEEVQALPRSSFAGDIHCVTRWSKLGTSFAGVSVDTLL
                     DAAEPLPAASHVVAFCYGGYTTNLPLDDLRGGKAWVVWEHEGAPLPPVHGGPARLLVP
                     HLYLWKSAKWLAGLRLLDHDAPGFWESLGYHNRGDPWREERYWGD"
     gene            1146..1823
                     /locus_tag="VFO47_05835"
     CDS             1146..1823
                     /locus_tag="VFO47_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET8788249.1"
                     /translation="MAELHVLRVFVAPDGTGGNPLGVFLAGPAVPQGRRQPVATDLGF
                     SETVFVDDPAAGGVRIFTPAAELPFAGHPLVGTSWLLARERTAVAVLRPPAGEVPTFL
                     DGDGVTWIRGRAEWAPEMAFRQLASPAEVDTLDGAPDGLGFADCWAWEDEAAGRIRSR
                     VFAPSYGIAEDEATGAAAVLLVSQLGRPVTIRQGAGSILHARPGPGGTAEVGGGVVLD
                     EVRDYRV"
     gene            complement(1811..2830)
                     /locus_tag="VFO47_05840"
     CDS             complement(1811..2830)
                     /locus_tag="VFO47_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8788250.1"
                     /translation="MDRIKRLVQAADDFQQRHRWVAFPVAVAKKYGEDQAGHRAALLA
                     YYGFFSLFPLLLVAVTVLGFVLQGQSDLGQRIVDSAVAQFPVIGDEISGTVSQSRLRG
                     SGLALAVGAALALWGGLGVAEAAQAAMNGIWNVPRRRYPNFFLRRLRGLAWLVSLGGG
                     LLLASVVSGFAAAADTAWSGPAVVAASTLVNMLLFLVGFRVLTVRNVALRSLLPGAVL
                     AALAWALLQWLGGWYVDRQLSRASATYGAFALVIGLLSWLYLASTVTLLAAEVNVVRT
                     RKLWPRSLAPPPLGGPDERALEGLARQEERLPDQRVEVSFGGDEDAGAAGERPAERGP
                     PAQTR"
     gene            complement(3101..>3323)
                     /locus_tag="VFO47_05845"
     CDS             complement(3101..>3323)
                     /locus_tag="VFO47_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8788251.1"
                     /translation="KTARGLVDTCDALALEQLRVAAMTAAAAGGVEHPGRNVAAKAGL
                     NRSILDAGWGQFTSILVAKAESADGEWSS"
BASE COUNT          439 a         1283 c         1206 g          395 t
ORIGIN      
        1 ggccagccgg tccagctcct cgcggaagat cacgtcctcg agggtccggg aggagtacag
       61 caggcgggcc gggagctggc tgccggtggc ggcccggtgg cgcagcatgg ccatcagcgg
      121 caccactccc gacccgccgg cgaccagcag cagcggcccg ccccgccccg gctcccagac
      181 gaagtagccg ccgaccgggc cgcgcagctc gagctggtcg ccctggcgga gctcgccggc
      241 cagatacggc gagacctcgc cgtcgtccag ccgctcgacg gccagctcga cccgctcgcc
      301 gccgggggcc gaggcgatcg agtaggcccg ctcggcctgg tagccgtcct cggcggtcag
      361 gcgcacgtcg acgtgctggc cgggcaggtg cccgtcccac tccggtacct caagggccag
      421 ggtgacgacc cggtcggtct cgggcctgac ctcggcgacc gtggccagcc gccagctcag
      481 tcgccccaat accgctcctc ccgccagggg tcgccccggt tgtggtagcc gagcgactcc
      541 cagaagccgg gggcgtcgtg gtcgagcagg cgcagcccgg ccagccactt ggcgctcttc
      601 cacaggtaca ggtggggcac gagcagccgg gccgggccgc cgtgcacggg cggcaggggg
      661 gcgccctcgt gctcccacac cacccaggcc ttgccgccgc ggaggtcgtc gaggggcagg
      721 ttggtggtgt agccgccgta gcagaaggcg acgacgtggc tggccgcggg cagcggctcg
      781 gccgcgtcca ggagggtgtc gaccgacacc ccggcgaagc tggtgccgag cttgctccag
      841 cgggtcacgc agtggatgtc gcccgcgaag ctggaccggg gcagcgcctg gacctcctcc
      901 caggtccact cctggggcgt ggccaccagc ccctcgacct tgaggcgcca ttcggccagg
      961 tcgatccggg gggtcggccc ggcggtgagc accgggaacc cgcgctcggc gtactggccg
     1021 ggcggcagct tggccgcctg ctccgtcccg gcgcgccggc ggcccctgaa ccctcgcgac
     1081 acgaacccgc ccacgtgcca cctccctccg atgcgcattc tcccccggat cgctagccta
     1141 cggccatggc cgagctgcac gtgctgcggg tgttcgtggc tcccgacggg accggcggga
     1201 acccgctggg ggtgttcctg gccggcccgg ccgtgcccca gggccgccgc cagccggtcg
     1261 ccaccgacct cggcttcagc gagacggtgt tcgtcgacga cccggccgcg ggcggggtgc
     1321 ggatcttcac cccggcggcc gagctgccgt tcgccggcca cccgctggtc ggcaccagct
     1381 ggctgctggc ccgggagcgg acggcggtgg cggtgctgcg gcctcccgcc ggcgaggtgc
     1441 ccaccttcct cgacggcgac ggggtcacct ggatccgcgg ccgggccgag tgggccccgg
     1501 agatggcctt ccgccagctg gccagccccg ccgaggtcga cacgctcgac ggcgctcccg
     1561 acggtctcgg cttcgccgac tgctgggcct gggaggacga ggccgccggg cggatccgct
     1621 cccgcgtgtt cgcccccagc tacggcatcg ccgaggacga ggccaccggc gcggccgccg
     1681 tcctcctggt cagccagctg ggccggccgg tgacgatccg ccagggcgcc gggtcgatcc
     1741 tgcacgcccg tcccggcccc ggcggcacgg ccgaggtcgg cggcggggtc gtgctcgacg
     1801 aggtccgcga ctaccgggtc tgagccggcg gtccccgctc ggccggccgc tcccccgccg
     1861 cccccgcgtc ctcgtcgccg ccgaagctga cctcgacccg ctggtcgggc aggcgctcct
     1921 cctgcctggc caggccctcg agggcgcgct cgtcgggacc gcccaggggc ggcggggcca
     1981 ggctgcgggg ccacagcttg cgggtccgga ccacgttcac ctcggccgcc agcagggtca
     2041 ccgtggaggc caggtagagc cacgacagca ggccgatgac cagcgcgaac gcgccgtagg
     2101 tggcgctggc ccggctgagc tggcggtcca cgtaccagcc gcccagccac tgcagcagcg
     2161 cccaggcgag cgcggccagc accgccccgg gcagcagcga ccgcagggcc acgttgcgga
     2221 ccgtcagcac ccggaacccg accaggaaca gcagcatgtt caccagggtc gaggcggcca
     2281 ccacggccgg ccccgaccag gcggtgtcgg cggcggccgc gaaccccgac acgacgctgg
     2341 ccagcagcag cccgcccccg aggctgacca gccaggccag gccgcgcagg cggcgcagga
     2401 agaagttcgg gtagcgccgc cggggcacgt tccagatgcc gttcatggcc gcctgggcgg
     2461 cctcggccac ccccagcccg ccccacaggg ccagggcggc cccgacggcc agggccagcc
     2521 cgctgccccg cagccggctc tggctcacgg tcccgctgat ctcgtccccg atcaccggga
     2581 actgggccac cgccgagtcg acgatccgct ggcccaggtc ggactggccc tggagcacga
     2641 acccgagcac ggtcacggcc accagcagca gcgggaacag cgagaagaac ccgtagtagg
     2701 ccagcagcgc cgcccggtgc ccggcctggt cctccccgta cttcttggcc acggccaccg
     2761 ggaaggccac ccaccggtgc cgctgctgga agtcgtcggc cgcctgcacg aggcgcttga
     2821 tacggtccat cgcagctggg tgtacccacg cgatcccgtg atagccccga cctgacctga
     2881 ccgaggagcc gagcgcggtg actgctctgc cggccggagc cggcagcttc tcaagccgcg
     2941 gaggcttgac cagagcccag cccggccctg gctgcgatat tgcgagctcc gttgaggtcg
     3001 gcgtccagct cgccatgggt ggggcagacg acgatctcct gccgaggccg gccacaactg
     3061 gccccgcagg tgtggcagtc gatcgaggtg aaggcggggc tcacgaggac cactcgccgt
     3121 cggcgctctc agccttggcg acaagaatgc tcgtgaactg gccccagccg gcgtccagga
     3181 tcgaccggtt cagcccagcc ttggcggcaa cgttacggcc cggatgctcc acgccgccgg
     3241 ccgccgcagc cgtcatggcg gccacacgga gctgttcaag ggcgagggcg tcgcaggtgt
     3301 cgaccagccc gcgggcggtc ttg
//
LOCUS       DASSWR010000353         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_155029,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000353 DASSWR010000000
VERSION     DASSWR010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155029"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VFO47_05850"
     CDS             complement(<1..116)
                     /locus_tag="VFO47_05850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HET8788252.1"
                     /translation="MRVGPGAYLLLSAILFAIGVTGVLIRRNAIVVFMCIEL"
     gene            complement(113..652)
                     /locus_tag="VFO47_05855"
     CDS             complement(113..652)
                     /locus_tag="VFO47_05855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET8788253.1"
                     /translation="MTPVLAATASEQAELIVFVVLAPLAVVTALGMVLTRNAVYSALL
                     LVVNFFCLAGFYVLLEAQFVAAVQVIVYAGAIMVLFLFVLMLLGIGNEEAVSETIKGQ
                     RPAAVLLTLGLLVTLVGALFAGVFDAAPASLAAANEGGNVQAVGRILFTRYTFAFETA
                     GILLVVAAVGALVLGKRQP"
     gene            complement(696..1298)
                     /gene="nuoI"
                     /locus_tag="VFO47_05860"
     CDS             complement(696..1298)
                     /gene="nuoI"
                     /locus_tag="VFO47_05860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET8788254.1"
                     /translation="MMPSEILKGLSVTFRSMFRKRNTVQYPEEKKPTSPRFHGRHQLN
                     RYPDGLERCIGCELCAYACPADAIYVQGADNTPEARYSVGERYAAIYQINYNRCIFCG
                     LCIEACPTRALTMTNEYELSSPTRQGLVYDKPDLLAAVPSGAKAPPHVDAEDGPPTIA
                     RYGPQASVSQESVEPVWALLREQETRQADRELPPDRTVPR"
     gene            complement(1295..2443)
                     /gene="nuoH"
                     /locus_tag="VFO47_05865"
     CDS             complement(1295..2443)
                     /gene="nuoH"
                     /locus_tag="VFO47_05865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET8788255.1"
                     /translation="MSGTIWDLFILVVKVVGVFAALMVTVLVTIWIERRVVAFMQTRV
                     GPNRVGPFGVLQSLADGIKLFFKEDVRPSNADKILYGLAPVIMMVPAFLAFTVIPFGF
                     AFGSERAQLADLNVGLLFVLAMGSLQVYGIVLAGWASGSNYPLLGAVRSSAQMISYEI
                     AQGLSLVGVFMTAGTLSAAGIVAAQAGDPLLGFIPRWYVIFQFPLFLVFLLSGIAETN
                     RAPFDLPEAESELVGGYHTEYSGVKFALFFLAEYLNIITVSAVATTAFLGGPDGPAPD
                     FLPWLWPILWFVAKVFLFIFLFIWLRATLPRLRFDRLMDFGWKVLIPVALAWVAVTAV
                     VLVVDINLPRPVLLGAIGVLLALLLLSFLLPSSDKGKADRTAPGGSTP"
BASE COUNT          460 a          832 c          958 g          317 t
ORIGIN      
        1 atcagctcga tgcacatgaa gaccacgatg gcattgcgcc ggatcagcac cccggtcacg
       61 ccgatggcga acaggatggc cgagaggagg aggtacgcgc cgggcccgac cctcatggct
      121 gccgcttccc cagcaccagg gcgccgacgg cggcaaccac cagcaggatc ccggcggtct
      181 cgaaggcgaa ggtgtagcgg gtgaacagga tccggcccac cgcctggacg ttgccgccct
      241 cgttggccgc ggccaggctg gccggggcgg cgtcgaacac cccggcgaac agggcgccga
      301 cgagcgtgac cagcagccca agggtgagca ggacggccgc tgggcgctgg cccttgatgg
      361 tctcggagac ggcctcctcg ttgccgatgc cgagcagcat cagcacgaac aggaacagga
      421 ccatgatcgc cccggcgtac acgatcacct gcacggcggc cacgaactgg gcctccagca
      481 gcacgtagaa cccggccagg cagaagaagt tgaccaccag cagcagggcc gagtagacgg
      541 cgttgcgggt caggaccatg ccgagggcgg tgaccacggc caggggggcg agcacgacga
      601 agacgatcag ctcggcctgc tcggatgcgg tggcggccag caccggcgtc acttgggcac
      661 caccttttcg atcgggggtg cgagcggcaa cgggatcacc tggggaccgt cctgtcgggg
      721 gggagctcgc ggtcggcctg gcgggtctcc tgctcgcgca gcagcgccca gaccggctcg
      781 accgactcct gggagacgga cgcctggggg ccgtagcggg cgatcgtcgg cggcccgtcc
      841 tcggcgtcga cgtggggcgg ggccttggcc ccggaaggca cggcggccag caggtcgggc
      901 ttgtcgtaga ccagcccctg gcgggtcggc gaggacagct cgtactcgtt ggtcatggtc
      961 agggcgcggg tcgggcaggc ctcgatgcag aggccgcaga agatgcagcg gttgtagttg
     1021 atctggtaga tggccgcgta ccgctccccg accgagtagc gggcctccgg ggtgttgtcg
     1081 gccccctgga cgtagatggc gtcggccggg caggcgtagg cgcacagctc gcagccgatg
     1141 caccgctcca ggccgtcggg gtagcggttg agctggtggc ggccgtggaa ccggggcgag
     1201 gtcggcttct tctcctccgg atactgcacg gtgttgcgct tgcggaacat gctccggaag
     1261 gtgaccgaca gccccttcag gatctcgctg ggcatcatgg ggtcgaacct ccgggggcgg
     1321 tgcggtccgc cttccccttg tccgaggagg gcagcaggaa cgagagcagc agcagggcga
     1381 gcaggacgcc gatggcgccg agcagcaccg ggcggggcag gttgatgtcg accaccagga
     1441 ccacggcggt gaccgcgacc caggccaggg cgaccgggat cagcaccttc cagccgaagt
     1501 ccatcagccg gtcgaagcgc agccggggca gggtcgcccg cagccagatg aacaggaaga
     1561 tgaacaggaa gaccttggcc acgaaccaca ggatcggcca gagccagggc aggaagtcgg
     1621 gcgccgggcc gtccgggccg ccgaggaagg cggtggtggc caccgccgac acggtgatga
     1681 tgttcaggta ctcggccagg aagaacaggg cgaacttcac ccccgagtac tcggtgtggt
     1741 agccgccgac cagctccgac tcggcctcgg ggaggtcgaa gggggcccgg ttggtctcgg
     1801 cgatgcccga cagcaggaac accaggaaca gcgggaactg gaagatgacg taccagcggg
     1861 ggatgaagcc gagcagcggg tcgcccgcct gggcggcgac gatcccggcc gccgacaggg
     1921 tgccggcggt catgaacacc ccgaccagcg acagcccctg ggcgatctcg tagctgatca
     1981 tctgggccga ggagcggacc gcgcccagca gcgggtagtt cgaccccgag gcccagccgg
     2041 cgagcacgat cccgtacacc tgcaggctgc ccatggccag cacgaacagt agcccgacgt
     2101 tgaggtcggc cagctgggcc cgctcgctgc cgaaggcgaa gccgaacggg atcaccgtga
     2161 aggccaggaa ggccgggacc atcatgatca ccggggccag gccgtagagg atcttgtcgg
     2221 cgttgctggg ccgcacgtcc tccttgaaga acagcttgat gccgtcggcc agggactgga
     2281 ggacgccaaa gggcccgacc cggttggggc cgacccgggt ctgcatgaag gcgaccaccc
     2341 gccgctcgat ccagatggtc accagcaccg tgaccatcag ggcggcgaac acgccgacga
     2401 ccttgacgac caggatgaac aggtcccaga tggtcccgct catgcggccg cctcctcgac
     2461 cgcggtgacc ttgacccgca gggcggggtc ggggccgagc aaggccgcca cggccaggtc
     2521 gggctggttg gccgggacga aggcgcagcc gggagcgacc gtcgcgg
//
LOCUS       DASSWR010000354         7836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_380870,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000354 DASSWR010000000
VERSION     DASSWR010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380870"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFO47_05870"
     CDS             <1..1398
                     /locus_tag="VFO47_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788256.1"
                     /translation="GSGVAFLDATVVNIALTTNGEDLYAGIASLQWVVNAYTLTLSGF
                     LLLGGSLGDHYGRRRVFVLGVVWFALASLLCGLAPTDEALIAARALQGVGGALLTPGS
                     LAIIEASFRREDRGPAIGAWSGLGGVAAAIGPFLGGWLVQAASWRLVFLINLPLAAVV
                     VWVSVRHVPESRDPGATGRLDWAGAALAAVGLAGVVFALTDGPAAGWTSPRILATGLA
                     GVAALAAFVLWERRTAAPMLPLDIFASRQFSAANAVTFVVYGALGGSLFLLPIQLQQV
                     VGWSPLASGVALVPVTLVMLLLSARAGRLASRIGPRLPMTVGPLLVAAGFALFTRIVP
                     GASYPVDVLPASLVYGLGLVLTVAPLTATVLAAAPAEHAGIASAVNNDVARTAGLLAV
                     AVLPVAAGISGADALEPAIFAAGFRSAMTIAAVLCAAGGALSWLTIRNPEPEPERDHH
                     LSCPLTAPSPAARRA"
     gene            1499..4687
                     /gene="fdh"
                     /locus_tag="VFO47_05875"
     CDS             1499..4687
                     /gene="fdh"
                     /locus_tag="VFO47_05875"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017602536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2042..2044,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HET8788257.1"
                     /translation="MWPALRQLRGNDRLGLSEAARSRRSAELTPRTAEADRVVRSVCP
                     YCAVGCGQRVFVKDEQIIQIEGDPDSPISRGRLCPKGSASKQLVTSPGRLTTVRYRRP
                     HGTEWEDLSLDQAMDMIADRVLATRAATWQDHDDEGRRVNRTLGMASLGGATLDNEEN
                     YLIKKLFTALGAIQIENQARIUHSSTVPSLGTSFGRGGATNFQQDLANSDCIVIQGSN
                     MAECHPVGFQWVVEAKARGAKVIHVDPRFTRTSALADLHVPIRAGSDIAFLGGIVRHI
                     LENGREFREYVASYTNAAVIVDEAFADTEDLDGLFSGFDPASGTYDPRSWQYDGMGVA
                     SNAGSRQRRIAGGETHGSGGAKLSHGNPPHTDPTLEHPRCVFQLLKRHFARYTPELVE
                     EVCGVPRERFLEVAEALCANSGRERTSAFAYAVGWTHHTNGVQFIRTAAIVQLLLGNM
                     GRPGGGIMALRGHASIQGSTDIPTLYNLLPGYLSMPIAGVHGSLEEYLDDVRLPAGFW
                     GRSDTYMVSLLKAWFGDRATADNGFCFDHLPKLTGDHSIFPAVMAMLDGTCKGFIVPG
                     ENPAVGSPNAGLHRKAMANLDWLVVRDLVETETASFWYNAPEIETGELRTADIGTEVF
                     LLPAAAHTEKDGTFTNTQRLLQWHRKAVEPVGDCRSELWFYYHLGRRIREKLAPSTDP
                     RDRPLLDLEWSYPVEGPHEEPSAAAVLREIGGVGPDGRALPGYLELKADGSTACGCWI
                     YSGVYADEVNQADRRKPGSEQSWVAPEWGWAWPYNRRILYNRASADPEGRPWSERKRY
                     VWWDPTQGRWTGHDNPDFGATTAPGYDPPDDATAERALDGRSPFVMQADGKGWLFVPN
                     GLDDGPLPAHYEPPESPVPNALYGRRTNPLMETYDRPGNRFNPPGSAAYPFVFTTYRL
                     TEHHTAGAMSRTLGYLAELQPEMFCEVSPELARLRGLVNGGWATIVTSRAAIEARVLV
                     TDRMRPLKVAGRVVHQVGLPYHWGRNGLATGDGANDLLPIVGDPNVHIMESKANTCDV
                     LPGRRPRGPALVELVEGHRARALAEQREEAGG"
     gene            4684..5451
                     /locus_tag="VFO47_05880"
     CDS             4684..5451
                     /locus_tag="VFO47_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8788258.1"
                     /translation="MTARMGFFTDTSICIGCKACEVACKEWNLVPEDGPLALTGDSYD
                     NTGALGADTWRHVAFVEQRRDDDGLRWLMSSDVCKHCSSAACLEVCPTGALFRTEFST
                     VVVQPDVCNGCGYCVPACPFGVIDRREDDGRAFKCTLCYDRLRDGLQPACATACPTDS
                     IQFGELDELRERARARVATLHDAGVAEARLYLEDEDDGVGGAGAFFLLLDEPEVYGLP
                     PDPVSTTRDLPTLWRAAALAAGTLAAAVAAAFVGGRR"
     gene            5538..6434
                     /gene="nrfD"
                     /locus_tag="VFO47_05885"
     CDS             5538..6434
                     /gene="nrfD"
                     /locus_tag="VFO47_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET8788259.1"
                     /translation="MRGSVVPEAEFRSYYGRPIIKAPVWTWEVPWYFFVGGLAGASAS
                     LGLGARLAGNDRLARNAWTVSAAGVTAGVPLLVSDLGRPERFPYMLRMFKVTSPLSVG
                     SWILAAMGPAAAGAAVSDRLGIFPGLGRVAETVAGLLGPALATYTGTLVADTAVPVWH
                     EAGRELPFVFAGSAAASAGAAAVLLTPAADAGPARRLVVAGALLELGAAQTMERRLGE
                     LGEPYHQGPAGRLARLARACTAAGAGLVALGGRRRAMAAAGSALLLAGSACERWAVYK
                     AGFQSAADPRYVVGPQRERLQQ"
     gene            6574..>7836
                     /locus_tag="VFO47_05890"
     CDS             6574..>7836
                     /locus_tag="VFO47_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788260.1"
                     /translation="MTRLLAYLPRGNTLDDRAWHKRHLFLQALLLLHLPCLFVFGVLM
                     GRTAWDTTVVLSVPAACLVLGHLIKHRRTASVLITAGLTYCSAVLVSFSNGSIEAHFH
                     FFIMIGFIALYQDWVPFLWNVVFTVLSHGLGSVARTDLIFNHPSGQTSPWVWSTIHGV
                     AVLAACCGVVIFWETTEREQRKTLDLTKQLADAEITRRRFTSDLLVNLARRNQSLLYR
                     QLGLINQLEDQAEDPDALADLFRLDHLATRIRRNAESLLVLSGEEPPRTWGRPVALVD
                     VVRAAIAETEDLDRVVFAVDERLAVFGNAVADLTHLVAELVENAVHFSPPEASVILRT
                     RPYLQTPGAHVLTVEDWGVGMRADDMAAANDLLATPQDVDLSVSQRLGLHVVARLAQR
                     HGIEVSLTPTPGCGVTAAVVLPPSLFSDP"
BASE COUNT          990 a         3017 c         2742 g         1087 t
ORIGIN      
        1 gggtcgggag tggcattcct cgacgccaca gtcgtcaaca tcgccctgac gaccaacggc
       61 gaggacctgt acgccggcat cgccagcctc cagtgggtgg tcaacgccta caccctcacc
      121 ctgtcggggt tcctgctgct cggggggtcc ctcggcgacc actacggccg ccggcgggtg
      181 ttcgtcctcg gggtcgtctg gttcgcgctg gcgtcgctgc tctgtggcct cgcccccacc
      241 gacgaggccc tgatcgccgc ccgcgccctc cagggcgtcg gcggcgccct gctcaccccg
      301 ggcagcctgg ccatcatcga ggccagcttc cggcgcgagg accgcggccc ggccatcggc
      361 gcctggtccg gccttggcgg ggtggcggcc gcgatcggcc cgttcctggg cggctggctg
      421 gtccaggccg cgtcctggcg gctcgtcttc ctcatcaacc tgcccctggc cgcggtggtg
      481 gtctgggtgt cggtgcgcca cgtgcccgag tcgcgcgacc cgggcgccac cgggcgcctc
      541 gactgggccg gcgccgccct ggccgccgtc ggcctggccg gggtcgtctt cgccctcacc
      601 gacgggcccg ccgccggctg gacctcgccc cggatcctgg ccacgggcct ggccggagtg
      661 gccgccctgg ccgccttcgt gctctgggaa cggcgcaccg cggcgcccat gctgccgctg
      721 gacatcttcg cctcgcgcca gttcagcgcc gccaacgccg tcaccttcgt ggtctacggg
      781 gccctcggcg ggtcgctgtt cctcctgccc atccagctcc agcaggtggt cggctggtcg
      841 ccgctggcct cgggggtggc gctggtcccg gtcaccctgg tcatgctgct gctgtcggcc
      901 cgggcgggcc gcctggcctc caggatcggg ccgcggctgc ccatgaccgt ggggccgctg
      961 ctcgtggccg ccgggttcgc gctgttcacc cgcatcgtcc cgggggcgag ctacccggtc
     1021 gacgtcctgc ccgcgtcgct ggtgtacggt ctcgggctgg tcctcacggt ggcgccgctc
     1081 accgcgaccg tgctcgccgc cgccccggcc gagcacgccg ggatcgcctc ggcggtcaac
     1141 aacgacgtcg cccggacggc cgggctgctg gccgtggccg tcctgcccgt cgcggccggc
     1201 atctcgggcg cggacgccct ggagccggcc atcttcgccg ccgggttccg gtcggccatg
     1261 acgatcgccg ccgtcctctg cgccgccggc ggggccctgt cgtggctcac catccgcaac
     1321 cccgagcccg agccggagcg cgaccaccac ctcagctgcc cgctgaccgc accctcgccg
     1381 gccgctcgac gcgcctgacc tcccggtgtg cgttcccgga ctccgtggta gaccggtacc
     1441 gttgcacgtg gcgtcgaccg gcgaggaggc ggtgcgggtg gtggcgaggc ggctcccggt
     1501 gtggccggcg ctgcgccagc tccgcgggaa cgaccgcctc ggcctgtccg aggcggcccg
     1561 gtcgcggcgg tcggccgagc tgacgccgcg gaccgccgag gccgaccggg tcgtcaggtc
     1621 cgtctgcccc tactgcgcgg tcggctgcgg ccagcgggtg ttcgtcaagg acgagcagat
     1681 catccagatc gagggcgacc ccgacagccc catctcccgg ggccggctct gccccaaggg
     1741 gtcggcgtcc aagcagctcg tcacctcccc gggccgcctg accacggtcc gctaccgccg
     1801 cccccacggg accgagtggg aggacctgtc cctggaccag gcgatggaca tgatcgccga
     1861 ccgggtgctc gccacccggg ccgccacctg gcaggaccac gacgacgagg gccggcgggt
     1921 caaccgcacc ctgggcatgg ccagcctcgg cggggcgacc ctcgacaacg aagagaatta
     1981 cctgatcaag aagctgttca ccgccctcgg ggcgatccag atcgagaacc aggcccgcat
     2041 atgacactcc tcgaccgtcc ccagtttggg gacctcgttc ggccgcggtg gcgccaccaa
     2101 cttccagcag gacctggcca acagcgactg catcgtcatc cagggctcca acatggccga
     2161 gtgccacccg gtcgggttcc agtgggtggt cgaggccaag gcccgcgggg ccaaggtcat
     2221 ccacgtcgac ccccgcttca ccaggaccag cgccctggcc gacctgcacg tgcccatccg
     2281 ggccggcagc gacatcgcct tcctgggcgg gatcgtccgc cacatcctgg agaacggccg
     2341 tgagttccgg gagtacgtgg ccagctacac caacgccgcc gtgatcgtcg acgaggcgtt
     2401 cgccgacacc gaggacctgg acggcctgtt ctccgggttc gacccggcca gcggcaccta
     2461 cgacccgcgc agctggcagt acgacggcat gggggtggcc agcaacgccg gctcgcgcca
     2521 gcgccgcatc gccggcggcg agacccacgg ctcgggcggg gccaagctgt cgcacggcaa
     2581 cccgccccac accgacccca ccctggagca cccgcgctgc gtcttccagc tgctcaagcg
     2641 gcacttcgcc cgctacaccc cggagctggt ggaggaggtc tgcggcgtgc cccgcgagcg
     2701 gttcctggag gtcgccgagg cgctctgcgc caactcgggg cgggagcgga ccagcgcctt
     2761 cgcctacgcc gtgggctgga cccaccacac caacggcgtc cagttcatcc gcaccgccgc
     2821 catcgtccag ctgctgctcg gcaacatggg ccggcccggc ggcggcatca tggccctgcg
     2881 cggccacgcc tccatccagg gctcgaccga catccccacc ctctacaacc tgctgcccgg
     2941 ctacctgtcc atgccgatcg ccggcgtgca cgggagcctg gaggagtacc tcgacgacgt
     3001 gcggctgccg gccgggttct ggggccgcag cgacacctac atggtcagcc tcctcaaggc
     3061 ctggttcggc gaccgggcca cggccgacaa cggcttctgc ttcgaccacc tgcccaagct
     3121 gaccggcgac cactcgatct tcccggccgt catggccatg ctggacggca cctgcaaggg
     3181 gttcatcgtc ccgggcgaga acccggccgt cggctcgccc aacgccggcc tgcaccgcaa
     3241 ggccatggcc aacctggact ggctggtggt gcgggacctg gtcgagaccg agacggcgtc
     3301 gttctggtac aacgcgcccg agatcgagac cggcgagctg cgcaccgccg acatcggcac
     3361 cgaggtgttc ctgctcccgg cggccgccca caccgagaag gacggcacct tcaccaacac
     3421 ccagcgcctg ctccagtggc accgcaaggc ggtcgagccg gtgggggact gccgctcgga
     3481 gctgtggttc tactaccacc tggggcgccg catccgcgag aaactggccc cctccaccga
     3541 cccaagggac cgcccgctgc tcgacctgga atggtcgtac ccggtcgagg ggccgcacga
     3601 ggagccgagc gcggccgcgg tgctgcgcga gatcggcggg gtcggcccgg acggccgggc
     3661 cctgcccggc tacctggagc tgaaggccga cggctcgacc gcctgcggct gctggatcta
     3721 ctcgggggtc tacgccgacg aggtcaacca ggccgaccgg cgcaagcccg ggtccgagca
     3781 gagctgggtc gcccccgagt ggggctgggc ctggccctac aaccggcgca tcctctacaa
     3841 ccgggcctcg gccgaccccg agggccggcc ctggtcggag cggaagcgct acgtctggtg
     3901 ggaccccacc cagggacgct ggaccggcca cgacaacccc gatttcgggg ccaccaccgc
     3961 ccccggctac gacccgcccg acgacgccac cgccgagcgg gcgctggacg gacgcagccc
     4021 gttcgtgatg caggccgacg gcaagggctg gctgttcgtg cccaacggcc tcgacgacgg
     4081 gccgctgccg gcccactacg agccgcccga gtcgccggtg cccaacgcgc tgtacggccg
     4141 ccggaccaac ccgctgatgg agacctacga ccggcccggc aaccgcttca acccgccggg
     4201 ctcggcggcg tacccgttcg tgttcaccac ctaccggctc accgagcacc acaccgccgg
     4261 ggcgatgagc cgcaccctgg gctacctggc cgagctccag ccggagatgt tctgcgaggt
     4321 cagcccggag ctggcccggc tccgggggct ggtcaacggc ggctgggcga cgatcgtcac
     4381 ctccagggcc gccatcgagg ccagggtcct ggtcaccgac cggatgcggc ccctcaaggt
     4441 cgccggccgg gtcgtccacc aggtcggcct gccctaccac tggggccgca acgggctggc
     4501 caccggggac ggggccaacg acctgctgcc gatcgtcggt gaccccaacg tccacatcat
     4561 ggagtccaag gccaacacct gcgacgtcct ccccgggcgc cggccccgcg gcccggctct
     4621 ggtcgagctg gtcgagggcc accgggcgag ggccctggcc gagcagcgcg aggaggcggg
     4681 cggatgaccg cgcggatggg gttcttcacc gacacctcga tctgcatcgg ctgcaaggcc
     4741 tgcgaggtgg cctgcaagga gtggaacctg gtcccggagg acgggccgct ggccctcacc
     4801 ggcgactcct acgacaacac cggggcgctg ggggccgaca cctggcgcca cgtcgccttt
     4861 gtcgagcagc ggcgcgacga cgacgggctg cgctggctga tgagctcgga cgtgtgcaag
     4921 cactgcagct cggcggcgtg cctggaggtg tgcccgaccg gggcgctgtt ccggaccgag
     4981 ttctccacgg tggtggtgca gcccgacgtc tgcaacggct gcggctactg cgtgcccgcc
     5041 tgcccgttcg gggtgatcga ccggcgcgag gacgacgggc gggccttcaa gtgcaccctc
     5101 tgctacgacc ggctgcgcga cgggctccag ccggcctgcg ccaccgcctg cccgaccgac
     5161 tcgatccagt tcggcgagct ggacgagctg cgcgagcggg cccgggcccg ggtggccacc
     5221 ctgcacgacg ccggggtcgc cgaggcccgg ctgtacctgg aggacgagga cgacggcgtc
     5281 ggcggggccg gggcgttctt cctgctgctg gacgagccgg aggtgtacgg cctgccgccc
     5341 gacccggtgt ccaccacccg cgacctgccc accctgtggc gggcggccgc cctcgccgcc
     5401 gggacgctgg ccgccgccgt ggccgccgcg ttcgtgggag gccgccggtg agccgcaacg
     5461 gcccagccgg gagcgggccg gtccctgacc ggccggaggg gtcgaggaga ccccgagggg
     5521 gaggcccggc gactcgcgtg cgcgggtcgg tggtgcccga ggccgagttc cgctcctact
     5581 acggccggcc gatcatcaag gcgcccgtct ggacctggga ggtcccctgg tacttcttcg
     5641 tcggcggcct ggccggagcc tcggcctcgc tcggcctggg ggcgcgcctg gcgggcaacg
     5701 accggctggc ccggaacgcc tggacggtgt cggccgccgg ggtgacggcc ggggtgcccc
     5761 tgctcgtctc cgacctcggc cgccccgagc gcttccccta catgctgcgg atgttcaagg
     5821 tgacctcgcc gctcagcgtc ggcagctgga tcctggccgc catgggcccg gccgccgccg
     5881 gcgccgccgt cagcgaccgg ctcggcatct tccccgggct gggccgcgtg gccgagacgg
     5941 tggccgggct gctcggcccg gccctggcca cctacaccgg caccctggtg gccgacacgg
     6001 ccgtgccggt gtggcacgag gccggccgcg agctgccgtt cgtgttcgcc gggagcgccg
     6061 ccgccagcgc cggcgcggcc gcggtgctgc tcaccccggc cgccgacgcc ggcccggccc
     6121 ggcgcctggt cgtggccggg gccctgctcg agctgggggc cgcccagacc atggagcggc
     6181 gcctgggcga gctcggcgag ccctaccacc agggcccggc cggccgcctg gcccgcctgg
     6241 cccgggcctg cacggccgcc ggggccgggc tggtcgccct gggcggccgc cgccgggcga
     6301 tggccgccgc cgggtcggcg ctgctcctgg ccgggtcggc ctgcgagcgc tgggcggtct
     6361 acaaggccgg cttccagtcg gccgccgacc ccaggtacgt ggtcgggccg cagcgcgaac
     6421 gactccagca gtgagacgag cggttcgcgc cgtctgggaa aattcctgaa ggttcgcgcg
     6481 gcacgcctgg tgaacgacga tttgcggctt cggcggcgaa agttacatga ttccgcggat
     6541 caccaccaga actagccggg ggaggggctc ggattgacca ggctgctcgc gtacctaccg
     6601 cggggcaaca cccttgacga cagggcctgg cacaaacggc acctgttcct acaggccctc
     6661 ctgctgctgc acctgccgtg cctgttcgtg ttcggggtgc tcatgggccg gaccgcctgg
     6721 gacaccaccg tcgtgctcag cgtccccgcc gcctgcctgg tcctcggcca tctgatcaag
     6781 catcgccgga cagcgtcggt gctgatcacg gccgggctca cctactgctc ggcggtgctg
     6841 gtgagcttct ccaacggctc catcgaagcc cacttccact tcttcatcat gatcggcttc
     6901 atcgccctgt accaggactg ggtgccgttc ctgtggaacg tggtcttcac cgtgctgagc
     6961 cacggcctcg gctcggtggc gcgcaccgac ctgatcttca accacccgtc cgggcagacc
     7021 agcccctggg tctggtcgac catccacggc gtggccgtgc tcgccgcctg ctgcggcgtg
     7081 gtcatcttct gggagacgac cgagcgcgag cagcgcaaga ccctggacct gaccaagcag
     7141 ctggccgacg ccgagatcac ccggcgccgc ttcacctccg acctgctggt caacctggcc
     7201 cggcgcaacc agagcctgct ctaccgccag ctcgggctca tcaaccagct cgaggaccag
     7261 gcggaggacc ccgacgccct ggccgacctg ttccggctcg accacctggc gacccggatc
     7321 cggcgcaacg ccgagagcct gctggtgctg tcgggcgagg agccgccccg gacctggggc
     7381 cggccggtgg ccctggtcga cgtcgtgcgg gcggccatcg ccgagaccga ggacctggac
     7441 cgggtggtgt tcgccgtcga cgagcggctg gccgtgttcg gcaacgccgt cgccgacctc
     7501 acccacctgg tggccgagct ggtcgagaac gccgtccatt tctcgccccc cgaggccagc
     7561 gtgatcctcc gcacccgtcc ctatctccag accccgggcg cgcacgtgct cacggtcgag
     7621 gactggggcg tcggcatgcg ggccgacgac atggcggccg ccaacgacct gctggccacc
     7681 ccccaggacg tcgacctgtc ggtgtcccag cggctggggc tgcacgtggt cgcccggctc
     7741 gcccagcgcc acggcatcga ggtgtcgctc acccccaccc cgggctgcgg cgtcaccgcc
     7801 gccgtggtgc tcccgccgtc gctgttctcc gacccc
//
LOCUS       DASSWR010000355         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1115233, whole genome shotgun sequence.
ACCESSION   DASSWR010000355 DASSWR010000000
VERSION     DASSWR010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115233"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFO47_05895"
     CDS             complement(<1..835)
                     /locus_tag="VFO47_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET8788261.1"
                     /translation="MTPGIDLERVADWFEANVPGVRPPLSFRQIAGGRSNLTYRVEDA
                     ARATWVLRRPPLHGVLPSAHDMGREHRVITALAGTPVPVPATFGLCQDPAVTGAPFYV
                     MAHVDGVVPRDEATVAAALDEPARAAAAGSLIDALVALHGVDPDQVGLGELGRRSGYL
                     ERQLARWQRQLEQSRTRPLPALDEVHRRLAAHLPVQVGPARIVHGDFRLDNVVLSPAG
                     QVLAVLDWELCTLGDPLADVGLLQVYWAEPGDRTLPLASAPTVMPGFPGRAAVAEAYA
                     AR"
     gene            complement(832..2226)
                     /locus_tag="VFO47_05900"
     CDS             complement(832..2226)
                     /locus_tag="VFO47_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET8788262.1"
                     /translation="MGIPSASYSITMRVHLGADPLGIGRITTAVGESAGTVVAVDIVE
                     SHPDLLVVDLTCNAVDARHADAITEAVGAIEGADVHAVSDRTFLLHLGGKLEVASKVP
                     LRTRDDLSMAYTPGVARVCRAIATNPADARKLTIKRNTVAVVTDGSAVLGLGNIGPEA
                     AMPVMEGKALLFKQFAGVDAWPVCLATQDTDEIVRTVELLAPGYGGVNLEDIAAPRCF
                     EVERRLRESLDIPVFHDDQHGTAIVVLAALANALRVVGKELGGVRVVLSGSGAAGVAI
                     IKILQAEGVGEIVACDRVGVLHKGRDGLDESKQWVAEHTNPSGQTGTLRDALAGADVL
                     VGVSGPGILDPADIATMAGDAIVFALANPDPEVDPAGARRHAAVVATGRSDEPNQINN
                     VLAFPGVFRGALDAGAHTITEAMKVAAARAIAARVHDDELRPEYIVPSVFDRGVAPGV
                     AEAVRHAAEEGPKG"
BASE COUNT          310 a          875 c          973 g          305 t
ORIGIN      
        1 accgggcggc gtaggcctcg gcgacggccg cccggccggg gaagccgggc atgaccgtcg
       61 gggccgaggc caatgggagg gtccggtcgc cgggctcggc ccagtacacc tggagcaggc
      121 cgacgtcggc cagcgggtcg ccgagggtgc acagctccca gtcgagcacg gccaggacct
      181 ggccggcggg ggagaggacg acgttgtcga gccggaagtc accgtggacg atccgggccg
      241 ggccgacctg gacggggaga tgggcggcca ggcggcggtg gacctcgtcg agcgcgggca
      301 ggggccgggt gcgggactgc tccagctggc gctgccagcg ggcgagctgg cgttccaggt
      361 agccggagcg gcggccgagc tcgcccaggc cgacctggtc gggatcgacc ccgtggaggg
      421 cgaccagggc gtcgatcagc gagccggcgg ccgccgcccg ggccggctcg tcgagggcgg
      481 cggcgacggt ggcctcgtcg cgggggacga cgccgtcgac gtgggccatc acgtagaagg
      541 gggcgccggt gacggccggg tcctggcaga ggccgaaggt ggccgggacc ggcaccgggg
      601 tgccggccag ggcggtgatc acccggtgct cgcggcccat gtcgtgggcc gaggggagca
      661 ccccgtgcag gggcgggcgc cgcagcaccc aggtcgcccg ggccgcgtcc tcgacccggt
      721 aggtgaggtt ggagcggccc ccggcgatct ggcggaagga aagcggcggc cgcaccccgg
      781 gcacgttggc ctcgaaccag tccgccaccc tctccaggtc gatcccgggg gtcacccctt
      841 tggtccctcc tcggcggcgt ggcggacggc ctcggccacc ccgggggcga ccccgcggtc
      901 gaacacgctg gggacgatgt actcggggcg cagctcgtcg tcgtgcacgc gggcggcgat
      961 ggccctggcc gcggccacct tcatggcctc ggtgatggtg tgggccccgg cgtccagggc
     1021 gccccggaac acgcccggga aggcgagcac gttgttgatc tggttgggct cgtcgctgcg
     1081 gccggtggcg accacggcgg cgtgacggcg ggcgccggcc gggtcgacct ccgggtcggg
     1141 gttggccagg gcgaacacga tcgcgtcgcc ggccatggtg gcgatgtcgg ccgggtcgag
     1201 gatgccgggc ccgctgaccc cgacgagcac gtcggcgccg gccagggcgt cgcgcagggt
     1261 cccggtctgg ccgctcgggt tggtgtgctc ggccacccac tgcttgctct cgtccaggcc
     1321 gtcgcggccc ttgtggagca ccccgacccg gtcgcaggcg acgatctcgc ccaccccctc
     1381 ggcctggagg atcttgatga tggcgacccc ggcggccccc gaccccgaca gcaccacccg
     1441 gaccccgccc agctccttgc cgaccacccg cagggcgttg gccagagcgg ccaggaccac
     1501 gatggcggtg ccgtgctggt cgtcgtggaa cacggggatg tcgagcgact cccgcagccg
     1561 gcgctcgacc tcgaagcagc ggggggcggc gatgtcctcc aggttgaccc cgccgtagcc
     1621 gggggcgagc agctcgacgg tgcggacgat ctcgtcggtg tcctgggtgg ccaggcagac
     1681 cggccaggcg tcgaccccgg cgaactgctt gaacagcagg gccttgccct ccatcaccgg
     1741 catggccgcc tcggggccga tgttgcccag cccgagcacg gccgagccgt cggtgaccac
     1801 ggccaccgtg ttgcgcttga tggtgagctt gcgggcgtcg gccgggttgg tggcgatcgc
     1861 ccggcacacc ctggccaccc ccggggtgta ggccatcgac aggtcgtcgc gggtgcgcag
     1921 cggcaccttg gaggcgacct cgagcttgcc gcccagatgc agcaggaagg tccggtcgct
     1981 gacggcgtgc acgtcggcac cctcgatcgc gccgaccgcc tcggtgatgg cgtcggcgtg
     2041 gcgggcgtcg acggcgttgc aggtcaggtc cacgaccagc aggtcggggt gggactcgac
     2101 gatgtcgacc gcgacaacgg tgcccgccga ctcgccgacc gcggtggtga tgcggccgat
     2161 gcccagcggg tcggccccca ggtgcacccg catggtgatc gagtaggagg cgctgggaat
     2221 gcccatgtga gctccttgga acggtgcagc agcggccgcg ggatttggga gtcagcctat
     2281 cccagagcgt gacccggcta cgctggcccg gtgaccgccc gcgaccaccg cctcccggcc
     2341 ccgctggccg tccccgaccc gggccggctc gacccggccc ggccggacta ccaggccatc
     2401 ctggccgccc acggccgggc cctcgaggcc ggcgaggacg gctacctcga cccggccacc
     2461 ggg
//
LOCUS       DASSWR010000356         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_945961,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000356 DASSWR010000000
VERSION     DASSWR010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945961"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFO47_05905"
     CDS             complement(<1..920)
                     /locus_tag="VFO47_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET8788263.1"
                     /translation="MARNTADLDALVGRTLGRRRYEVVARIAQGGMAIVYRGHDRQLD
                     RVVAIKVPRPEFARDRGFSDQFRREARTAARLSHPNVVAVYDSGEERGLPWIVMELVS
                     GRTLRDLLDSQRQLSPETTAELLGPVADALDHAHHAGVVHLDVKPENLLLTSETVKVA
                     DFGLVRAAAQRGHGQALAATVHYCAPEVLRGGVVDGRADVYALGVVAWECVSGRPPFE
                     GDPRQVVQQHLGGRVPPPSRVVGGIPEPFDTAVARATDPDPTRRFLRASDFAAAMGAP
                     RRRRVDETLRATTLVPPAGPDTTAAWAPAAG"
     gene            1050..1382
                     /locus_tag="VFO47_05910"
     CDS             1050..1382
                     /locus_tag="VFO47_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788264.1"
                     /translation="MAFDDELGLLGYQLAQEKRDGTRTFSLARNRYLTYWLHLPADDA
                     AALFTWEFAIGEYMDEYGLQVGANEPLNQFLFPQIDTEVAQDMAEVVKAMERTEAMFA
                     GMNFGHRG"
     gene            1438..1608
                     /locus_tag="VFO47_05915"
     CDS             1438..1608
                     /locus_tag="VFO47_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788265.1"
                     /translation="MRRGGSLLITILLLLAAFWAGVQYERSNCRIDLPNTTEQVDESV
                     KCRDYQGTDLPG"
     gene            1691..2026
                     /locus_tag="VFO47_05920"
     CDS             1691..2026
                     /locus_tag="VFO47_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HET8788266.1"
                     /translation="MFGRATYLVLMAACLLVTLPLELVLKAGVYRRPRRWLAALVPVF
                     LVFNLWDWVAIRRGHWTYSARYTTGIELLGLPLEELVFFVVVPTCALLTYQAVVRLLP
                     APDRRRASR"
BASE COUNT          268 a          777 c          776 g          286 t
ORIGIN      
        1 ccggccgcgg gcgcccaggc ggcggtggtg tcggggccgg ccggcgggac cagggtggtg
       61 gcccgcaggg tctcgtcgac ccggcggcgg cggggcgccc ccatggcggc ggcgaagtcc
      121 gaggcgcgca ggaaccggcg ggtcgggtcg gggtcggtgg cccgggccac ggcggtgtcg
      181 aacggctccg ggatcccgcc gaccacgcgc gagggcggcg gcacccggcc gcccaggtgc
      241 tgctggacga cctggcgggg gtcgccctcg aacggcggcc ggccgctcac gcactcccag
      301 gcgaccaccc cgagggcgta gacgtcggcc cgcccgtcga ccaccccgcc ccgcagcacc
      361 tcgggggcgc agtagtggac ggtggcggcc agcgcctgcc cgtggccgcg ctgggcggcc
      421 gcccggacca gcccgaagtc ggccaccttg accgtctcgc tggtcaagag gagattttcc
      481 ggcttgacgt cgaggtggac cacgccggcg tggtgggcat ggtcgagggc gtcggcgacc
      541 gggccgagca gctcggcggt ggtctcgggg ctgagctgcc gctggctgtc gagcaggtcg
      601 cgcagggtgc ggccgctgac gagctccatc acgatccagg gcaggccccg ctcctcgccc
      661 gagtcgtaca cggcgacgac gttggggtgg gacaggcggg ccgccgtgcg ggcctcgcgg
      721 cggaactggt cggagaagcc gcggtcgcgg gcgaactcgg ggcgggggac cttgatcgcc
      781 accacccggt cgagctgccg gtcgtggccg cggtagacga tggccatgcc gccctgggcg
      841 atgcgggcga cgacctcgta gcggcggcgg cccagcgtcc ggccgacgag ggcgtccagg
      901 tcggcggtgt ttctggccaa ggtcagtccc aggatcggtc gccacccggc ggacaccggc
      961 cggccagaga attctaagca ggcaggaccc acaacgcacg gaggcgcagc gctggcgctt
     1021 cccggcccgg cccgcgagaa tgctccgaca tggccttcga cgacgagctg ggactgctcg
     1081 gctaccagct ggcccaggag aagcgggacg gcacccgcac cttctccctg gcccgcaacc
     1141 gctacctcac ctactggctg cacctgcccg ccgacgacgc ggccgccctg ttcacctggg
     1201 agttcgccat cggcgagtac atggacgagt acggactcca ggtcggcgcc aacgagccgc
     1261 tcaaccagtt cctgttcccc cagatcgaca ccgaggtggc ccaggacatg gccgaggtcg
     1321 tcaaggcgat ggagcggacc gaggcgatgt tcgccggcat gaacttcggc caccgcgggt
     1381 gagggcggcc attggggtag gctccgcccc ccaggcccac ccgtccgagg aggcagcatg
     1441 cgccgcggtg gttcgctgct catcacgatc ctgctgctcc tggccgcgtt ctgggccggt
     1501 gtccagtacg agcggagcaa ctgccggatc gacctgccga acaccaccga acaggtcgac
     1561 gagtcggtca agtgccgcga ctaccagggt accgacctgc ccggctagga cagtcgcgcg
     1621 gtggccggcg ggccgcccag gagccggcga cggccccagg cggcccgccg gctcctagga
     1681 ggccggggcc gtgttcgggc gcgccaccta cctggtcctg atggccgcct gtctgctggt
     1741 caccctgccc ctggagctgg tcctcaaggc gggggtgtac cgccgccccc ggcgctggct
     1801 ggccgccctg gtcccggtgt tcctcgtctt caacctgtgg gactgggtcg ccatccgccg
     1861 cggccactgg acctacagcg cccgctacac caccggcatc gagctgcttg gcctccccct
     1921 ggaggagctg gtcttcttcg tggtcgtccc gacctgcgcc ctgctcacct accaggccgt
     1981 cgtccgcctc ctccccgccc ccgaccgccg ccgggcgtcc cggtgatcgg ggagtacacc
     2041 ctcgcctcgg cctgcggggt cgtgctcgcc gtcctgctcg agctgctggt cctgcgcacc
     2101 ggcctgt
//
LOCUS       DASSWR010000357         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_747479,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000357 DASSWR010000000
VERSION     DASSWR010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747479"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VFO47_05925"
     CDS             complement(<1..1132)
                     /locus_tag="VFO47_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET8788267.1"
                     /translation="MSDEGEKVTAGGALDQLPDPDPDETSEWLDSLGDVVEREGPGRA
                     RFLLRKVLDHAGTLDVGLPPLPSTDYVNTIPASAEPEYPGDEELERRLLAIVRWNAAA
                     IVSRANRPELGVGGHMASYASAAELFEVGFNHFFRGKEGGAGDQIFFQGHSAPGVYAR
                     AFLEGRLTEANLDAFRQESKPGGLSSYPHPRLMPEFWEFPTVSMGLGPLNAIYQARFN
                     RYLHHREVADTSGSHVWAYIGDGEMDEPEAQGALTVAGRERLDNLTFVINCNLQRLDG
                     PVRGNGKIIQELEALFRGAGWNVIKVVWGRLWDDLLARDETGELVAKLNSTPDGQFQT
                     LAAESPAYIREHLFDTKGLRRLTEGLSDAEVVALAVDRGGHDL"
     gene            1204..1842
                     /locus_tag="VFO47_05930"
     CDS             1204..1842
                     /locus_tag="VFO47_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET8788268.1"
                     /translation="MTGVIEVLWLGMLLGVGAALSVGPIFVVIVQQAATSGFPAAFRV
                     ILGSATADLVLLVPAIAFAWIIRSLERGALWVGLTGAAFLLYLAWQAAGDAVALWHAD
                     RRLVADDRWAFWKGVAGNLANPLTWTFWLATGTPALLAAQRAGGAAGVVVFCATWFLV
                     ASGLEAVIALGVARSGRRVGTRGQAWFTAVSAVAFLCLAATLVARDVLPRLG"
     gene            complement(1865..>2708)
                     /locus_tag="VFO47_05935"
     CDS             complement(1865..>2708)
                     /locus_tag="VFO47_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8788269.1"
                     /translation="KVAFDRGVPPVRLAEARVLVALLALAPFLVWRRPDLLRPPPGTW
                     PALAGFGASVAAVNLTYYVAIDHLPVGVALALQYTGPAMLLAVTTLTPRDQESRPGRL
                     AWAAAGLTLAGAVLVSRALEGLGALDLAGLAAGLASAVLFATYLLSAELAGRRGAEPA
                     TTLLWGFLVAVAIWSLTVPWWSWPVATLADPGVVAAVLGVGLVGTLVPFALAVGAVRV
                     ISASTAGIAATSEPVFAVAFAWVLLGQHLNPAQLAGAALVVAGVVLAQLAAARSRPGG
                     PGAS"
BASE COUNT          335 a         1014 c         1008 g          351 t
ORIGIN      
        1 gcaggtcgtg gccgccccgg tccacggcca gggcgaccac ctcggcatcc gacagcccct
       61 cggtgaggcg cctcagcccc ttggtgtcga acaggtgctc gcggatgtag gccgggctct
      121 cggcggccag ggtctggaac tgcccgtccg gggtcgagtt cagcttggcc accagctcgc
      181 cggtctcgtc ccgggcgagg aggtcgtccc agagccggcc ccagacgacc ttgatgacgt
      241 tccagccggc cccccggaac agcgcctcca gctcctggat gatcttgccg ttgccccgga
      301 ccgggccgtc caggcgctgc aggttgcagt tgatcacgaa ggtcaggttg tccagccgct
      361 cccggccggc cacggtcagc gccccctggg cctcgggctc gtccatctcg ccgtcgccga
      421 tgtacgccca cacgtgcgac ccggaggtgt cggccacctc ccggtggtgc aggtagcggt
      481 tgaagcgggc ctggtagatg gcgttgaggg ggccgaggcc catggagacg gtggggaact
      541 cccagaactc gggcatgagc cgcgggtgcg ggtaggacga caggccgccg ggcttgctct
      601 cctggcggaa ggcgtccagg ttggcctcgg tgagccggcc ctccaggaac gcccgcgcgt
      661 acaccccagg ggccgagtgg ccctggaaga agatctggtc gccggcgccg ccctccttgc
      721 cacggaagaa gtggttgaag ccgacctcga acagctcggc cgccgaggcg tacgaggcca
      781 tgtgcccgcc gacgcccagc tcgggccggt tggcccgcga cacgatggcg gccgcgttcc
      841 agcgcacgat cgccagcagc cgccgctcca gctcctcgtc gcccgggtac tcgggctcgg
      901 ccgaggccgg gatggtgttg acgtagtcgg tcgagggcag cgggggcagg ccgacgtcga
      961 gggtgccggc gtggtcgagg accttgcgga ggaggaagcg tgcccggccc gggccttcgc
     1021 gttcgacgac gtcgcccagc gagtccagcc attcgctggt ctcgtccgga tcggggtcgg
     1081 gaagctggtc cagcgccccg cccgcggtga ccttctcgcc ctcgtcgctc accgtcggct
     1141 ccctgggatc ggggggattg tcctctccaa cccacacctt agtcgccgtc gccgaccact
     1201 accatgaccg gcgtgatcga ggtgctgtgg ctggggatgc tgctgggcgt gggcgccgcc
     1261 ctgtcggtcg gtcccatctt cgtggtcatc gtccagcagg ccgccaccag cgggttcccg
     1321 gccgccttcc gggtcatcct cgggtcggcc accgccgacc tcgtcctgct ggtcccggcc
     1381 atcgccttcg cctggatcat ccggtcgctg gagcgggggg cgctgtgggt cgggctgacc
     1441 ggggccgcgt tcctcctcta cctggcctgg caggcggccg gcgacgccgt ggccctctgg
     1501 cacgccgacc gccggctggt cgccgacgac cgctgggcgt tctggaaggg ggtcgccggg
     1561 aacctggcca accccctcac ctggaccttc tggctggcca cggggacgcc ggcgctgctg
     1621 gcggcccagc gggccggcgg cgcggccggg gtggtggtct tctgcgccac ctggttcctg
     1681 gtcgcctcgg gcctggaggc ggtgatcgcc ctcggcgtgg cccgctccgg gcggcgggtc
     1741 gggacccgcg ggcaggcctg gttcacggcc gtgtcggcgg tggccttcct ctgcctggcc
     1801 gcgaccctgg tcgcccgcga cgtcctcccg cgcctcggct aggcctgatc ctccgggggc
     1861 ctagtcatga ggcccccgga ccccccggcc gggaccgggc ggcggccagc tgggccagga
     1921 cgaccccggc cacgaccagg gcggccccgg cgagctgggc cgggttgagg tgctggccca
     1981 gcagcaccca ggcgaacgcg accgcgaaca ccggctcgga ggtggcggcg atgccggcgg
     2041 tggaggcgct gatcacccgg accgccccga cggccagggc gaacggcacc agggtgccga
     2101 ccaggccgac cccgagcacg gccgccacca ccccggggtc ggccagggtg gccaccggcc
     2161 acgaccacca gggcacggtc agcgaccaga tggccaccgc gaccaggaac ccccacagca
     2221 gggtggtcgc cggctcggcc ccgcggcggc cggccagctc ggccgagagc aggtaggtgg
     2281 cgaacagcac ggccgaggcc agcccggcgg ccagccccgc caggtcgagc gcccccagcc
     2341 cctcaagggc ccggctgacc agcacggccc cggccagggt gagcccggcc gccgcccagg
     2401 ccagccgccc cggccgggac tcctggtccc gcggcgtcag ggtggtcacg gccagcagca
     2461 tcgccgggcc ggtgtactgc agggccaggg cgaccccgac cggcaggtgg tcgatggcca
     2521 cgtagtaggt caggttgacc gcggccacgc tggccccgaa cccggccagg gccggccagg
     2581 tccccggcgg cggccgcagc aggtcgggcc gccgccagac caggaacggg gccagggcca
     2641 gcagcgccac caggaccctg gcctcggcca ggcgcaccgg cggcacgccc cggtcgaagg
     2701 cgaccttg
//
LOCUS       DASSWR010000358         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_493794,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000358 DASSWR010000000
VERSION     DASSWR010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493794"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VFO47_05940"
     CDS             <1..1209
                     /locus_tag="VFO47_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003943149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HET8788270.1"
                     /translation="RLQRLVSVRVGRMMASPYAFLRGSAALMAEDFAHLPRTGIEPVI
                     CGDAHLGNFGFYASPERYLVFDLNDFDEAHPGPWEWDLYRLATSVWVAGRQNGLSEDT
                     CEDAALRCAYAYRRQLRQLAGQPLLPRSFDRLDIDRMRAELADWSLRGEIERAATRAR
                     TRTSDRALPKLTRERGGARHIVDQPPLVTHVSDRETGQLIEGLERYLDTLPPHWRRVL
                     AYYRVVDLAHKVVGVGSVGLRAYVALLQGNDPNDVVFLQLKQARRSCVARFVHGDTAW
                     HAHQGQRVVEYQQTLQTVSDPLLGWTSVGERDYYVRQFRDMKGAITVDGMDGSSLLDY
                     ARVCGTLLAKGHARSTGATRLSAYLGKGDSADRAFARFARAYADQTEADHEALGKAVA
                     AGRLPAEAGI"
     gene            1212..2342
                     /locus_tag="VFO47_05945"
     CDS             1212..2342
                     /locus_tag="VFO47_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET8788271.1"
                     /translation="MDQPRGQAERVSTLELFFDLVFVFTITQLTAVLSDDPTPRGLLR
                     VVLMLGVIYYMYGGYAWLTNAVAADRAARRLLLLGGMAGFLVLALAIPRAFSAGDVAF
                     GVAYLVVVAVHAGLFTRAGATHTVRAVIRLAPFNLAAALLVLAAVLVQGTPAYLLWAA
                     AFALEWLTPVLAGTGGYRIAPAHFVERHGLVVIVALGESIVAIGIGAADLPVDLALAS
                     VAVLGLLLAACLWWAYFGGDDDTRAERALAAVPDDRRGWVAIQAFGYWHLVMLLGIIA
                     MAAGLKDAIGHAFDPLDLAHALMLSGGAALFLAGDLLFRRTLQLGPAALRATAAALAL
                     ATVPVGLTLSALAQLTTLVLVLAAALTLERRAARPSDRQPRR"
BASE COUNT          271 a          970 c          817 g          350 t
ORIGIN      
        1 cggctgcagc ggctggtgtc ggtgcgggtc gggcggatga tggcctcgcc gtacgcgttc
       61 ctgcgcggct cggccgccct gatggccgag gacttcgccc acctgccccg caccgggatc
      121 gagccggtca tctgcggcga cgcccacctt ggcaacttcg gcttctacgc ctcccccgag
      181 cggtatctgg tcttcgacct caacgacttc gacgaggccc accccgggcc ctgggagtgg
      241 gacctgtacc ggctggccac cagcgtgtgg gtggccggcc gccagaacgg cctgtccgag
      301 gacacctgcg aggacgcggc cctgcgctgc gcctacgcct accggcggca gctgcgccag
      361 ctggccggcc agcccctgct gccccggtcc ttcgaccgcc tcgacatcga ccggatgcgg
      421 gccgagctgg ccgactggtc gctgcgcggg gagatcgagc gggcggccac gcgggcccgg
      481 acccggacca gcgaccgggc cctgcccaag ctgacccgcg agcgcggcgg ggctcgccac
      541 atcgtcgacc agccgccgct ggtcacccac gtctccgacc gtgagaccgg ccagctgatc
      601 gaggggctgg agcggtacct ggacaccctg ccgccgcact ggcggcgggt gctcgcctac
      661 tacagggtgg tcgacctggc ccacaaggtg gtcggggtcg gcagcgtcgg gctgcgcgcc
      721 tatgtcgccc tgctccaggg caacgacccc aacgacgtcg tgttcctcca gctcaagcag
      781 gccaggcgct cctgtgtggc caggttcgtg cacggggaca cggcctggca cgcccaccag
      841 ggccagcggg tggtcgagta ccagcagacc ctgcagacgg tcagcgaccc cctgctcggc
      901 tggaccagcg tgggcgagcg cgactactac gtgcgccagt tccgcgacat gaagggggcg
      961 atcaccgtcg acggcatgga cgggtcgtcc ctgctcgact acgcccgggt ctgcggcacc
     1021 ctgctggcca agggccacgc ccgctccacc ggggcgacca ggctgtccgc ctacctgggc
     1081 aagggcgaca gcgccgaccg ggccttcgcc aggttcgccc gggcctacgc cgaccagacc
     1141 gaggccgacc acgaggcgct gggcaaggcc gtcgccgccg gccgcctccc ggcggaggcc
     1201 gggatctgac ggtggaccag ccccgcgggc aggccgagcg ggtctccacc ctggagctct
     1261 tcttcgacct ggtcttcgtg ttcaccatca cccagctgac ggccgtgctg agcgacgacc
     1321 cgaccccgcg ggggctgctg cgggtcgtcc tcatgctggg cgtcatctac tacatgtacg
     1381 gcggctacgc ctggctgacc aacgccgtcg ccgccgaccg ggcggcgcgg cggctgctgc
     1441 tgctcggcgg catggccggc ttcctggtcc tggccctggc catcccgcgg gccttctcgg
     1501 ccggggacgt cgccttcggg gtcgcctacc tggtggtcgt ggccgtccac gccgggctgt
     1561 tcacgcgcgc cggggccacc cacacggtcc gggcggtcat ccgcctggcc ccgttcaacc
     1621 tcgccgccgc cctgctcgtc ctggccgccg tgctggtcca gggcacgccc gcctacctgc
     1681 tgtgggcggc cgcgttcgcg ctggagtggc tcacccccgt gctcgccggc accggcggct
     1741 accggatcgc cccggcccac ttcgtcgagc gccacggcct ggtggtgatc gtcgccctcg
     1801 gcgagtcgat cgtggccatc ggcatcggcg ccgccgacct cccggtcgac ctggccctgg
     1861 ccagcgtggc cgtgctcggc ctgctgctgg ccgcctgcct gtggtgggcc tacttcggcg
     1921 gcgacgacga cacccgggcc gagcgggccc tggccgccgt gcccgacgac cgccgcggct
     1981 gggtcgccat ccaggccttc ggctactggc acctggtcat gctgctgggg atcatcgcca
     2041 tggccgccgg cctcaaggac gccatcggcc acgccttcga ccccctcgac ctggcccacg
     2101 ccctcatgct gtcgggcggg gccgccctct tcctggccgg cgacctcctc ttccgccgca
     2161 ccctccagct cggccccgcc gccctccgcg ccaccgccgc cgccctcgcc ctggccaccg
     2221 tccccgtcgg cctcaccctc tccgccctcg cccagctgac caccctggtc ctggtcctgg
     2281 ccgccgccct cacgctggaa cgccgggccg cccggccatc cgaccgtcag ccgcgccggt
     2341 agacgtcggc ccggtggtcg atcaccagga cctcgaccct ccggcgccgg tcgtcgatcc
     2401 ggtacacg
//
LOCUS       DASSWR010000359         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_465231,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000359 DASSWR010000000
VERSION     DASSWR010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465231"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..243)
                     /locus_tag="VFO47_05950"
     CDS             complement(16..243)
                     /locus_tag="VFO47_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788272.1"
                     /translation="MTDTTRVEDEDIVTTWREDAKGAGSAPTATVADPQDADGTDGDG
                     TDGTDGDSGDGDSDGSDGDSADGTDGDSVDQ"
     gene            complement(315..1754)
                     /locus_tag="VFO47_05955"
     CDS             complement(315..1754)
                     /locus_tag="VFO47_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HET8788273.1"
                     /translation="MVPFEELQRSFAARYPASDPLDLGEGTVVVLPSLSFPTAELRKI
                     VGIGYYEERLLFLLLLLRRPRVKVVYLTSMPVEEAVVDYYLSFLPDPAGARSRLHMLA
                     AGDPAPRSLTAKLLDRPELLDRVRELCDGPGGAFVQPFNVTPGEQELSARLGLPLYGP
                     PAALAGLGSKTGSRRVARLAEVAVLDGAEDLRSVEAVEAEARRLRRRGVPPVGAVVIK
                     LNDGFSGQGNAVVDLAGLNGSLLDSPVVFCAEEESWPSFAAKIAEGGAVVEELLDVPG
                     MVSPSVQLRVLPGGTPLVLSTHSQVLGGPNRQVYLGCRFPADEAYRGEIKAAAERVGR
                     VLAARGVIGYFGIDFFVVPGGGRERVFLSEINLRVGGTTHPFGMAKLVTDGRYDAADG
                     HLVAAGQAKAYLATDNLKAASLVGCQPQEVIDRVDGLGFGYDHRRRTGTTLHLLGALR
                     EYGKMGVTCIGDTEDEARQRYRAVVAALT"
     gene            1892..2275
                     /locus_tag="VFO47_05960"
     CDS             1892..2275
                     /locus_tag="VFO47_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HET8788274.1"
                     /translation="MTQATTFYDAVGGEPAFRRLVAGFYRRVATDPVLRPLYPEEDLG
                     PAEERLRLFLIQYWGGPNTYSQRRGHPRLRMRHVPFVIGLAERDAWLRHMRVAVDELD
                     LAPELEKMLWDYFTMAANSMVNAPT"
     gene            2294..>2665
                     /locus_tag="VFO47_05965"
     CDS             2294..>2665
                     /locus_tag="VFO47_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET8788275.1"
                     /translation="MRRSPWWRHAVVYEVYVRSYADANGDGEGDLRGVRSRLGHLCEL
                     GVDALWLTPFYPSPLADGGYDVADYRDVDPRFGTLDDFDALVADAHEHGLRIIVDIVP
                     NHSSDRHPWFREAVAAPPGSSA"
BASE COUNT          364 a          988 c          936 g          377 t
ORIGIN      
        1 tcgatcgcgg ggttgctact ggtcgacgct gtcgccatcg gtgccgtcgg cgctgtcgcc
       61 gtcgctgccg tcgctgtcgc cgtcgccgct gtcgccgtcg gtcccgtcgg tgccgtcacc
      121 gtcggtgccg tcggcgtcct gggggtcggc cacggtggcc gtcggcgccg agccggcacc
      181 cttggcgtcc tcgcgccagg tggtgacgat gtcctcgtcc tcgacacgcg tggtgtcggt
      241 catggccgtc ctcctcgggc tcggctgctg ggacggctac gctagcaccc cggagccagg
      301 cgaaacagcg gagctcaggt gagggccgcg accaccgcgc ggtagcgctg cctggcctcg
      361 tcctcggtgt cgccgatgca ggtcacgccc atcttgccgt actcgcggag cgcccccagc
      421 aggtgcaggg tggtgccggt gcggcggcgg tggtcgtagc cgaacccgag gccgtcgacc
      481 cggtcgatga cctcctgggg ctggcagccg accagcgatg ccgccttgag gttgtcggtg
      541 gccaggtacg ccttggcctg cccggccgcc accaggtgcc cgtcggcggc gtcgtagcgc
      601 ccgtcggtga ccagcttggc catgccgaac gggtgggtgg tgccgccgac gcggaggttg
      661 atctcggaca ggaacacccg ctcccttccc cccccgggca cgacgaagaa gtcgatcccg
      721 aagtagccga tgaccccgcg ggcggccagg acccggccca cccgctcggc cgcggccttg
      781 atctcccccc ggtaggcctc gtcggccggg aagcggcagc ccaggtagac ctggcggttg
      841 gggccgccca ggacctggct gtgggtggac aggaccagcg gcgtcccgcc gggtaggacc
      901 cgcagctgca cgctcgggga gaccatgccg gggacgtcca gcagctcctc cacgacggcg
      961 ccgccctcgg cgatcttggc cgcgaacgac ggccacgact cctcctcggc gcagaacacc
     1021 accggcgagt ccagcagcga gccgttgagg ccggccaggt cgaccacggc gttgccctgg
     1081 ccggagaagc cgtcgttgag cttgatgacg accgccccga ccggcggcac gccccggcgg
     1141 cgcagccggc gggcctccgc ctccaccgcc tcgaccgagc gcaggtcctc ggccccgtcc
     1201 agcaccgcca cctcggccag ccgggcgacc cggcgcgagc cggtcttgga gccgaggccg
     1261 gccagggccg ccggcggccc gtacaggggc agcccgagcc gcgccgacag ctcctgctcg
     1321 ccgggggtga cgttgaaggg ctgcacgaac gccccgcccg ggccgtcgca cagctcccgc
     1381 acccggtcca gcagctcggg ccggtccagc agcttggccg tgagcgaccg gggggccggg
     1441 tcgccggcgg ccagcatgtg cagccgcgac cgcgcccccg ccgggtcggg caggaagctc
     1501 aggtagtagt cgaccaccgc ctcctcgacc ggcatcgagg tcaggtacac gaccttgacc
     1561 ctgggccggc gcagcagcag cagcaggaac agcagccgtt cctcgtagta gccgatgccg
     1621 acgatcttgc gcagctcggc cgttgggaag ctcaggctgg ggagcaccac caccgtgccc
     1681 tccccgaggt cgagcgggtc gctcgccggg taccgggcgg cgaagctgcg ctgcagctcc
     1741 tcgaatggaa ccaacggggg ggctgaccac atagctaaag ataaccagtc atagatggtg
     1801 cgcgcatccc ccgatttctc cggatcgtca tcttctacct actcgccacg tacggcggcc
     1861 aggcgaccgc cggagccggc atcatggaac catgacccag gcgacgacct tctacgacgc
     1921 ggtcggcggc gagccggcct tccggcggct ggtcgccggc ttctaccggc gcgtggccac
     1981 cgacccggtc ctgcgccccc tctaccccga ggaggacctc ggcccggcgg aggagaggct
     2041 gcgcctgttc ctgatccagt actggggcgg cccgaacacc tacagtcagc ggcgagggca
     2101 tccgcggctg cggatgcggc acgtgccgtt cgtgatcggc ctggccgagc gggacgcgtg
     2161 gctgcggcac atgcgcgtcg ccgtggacga actggacctg gcccccgagc tggagaagat
     2221 gctctgggac tacttcacga tggccgcgaa ctccatggtg aacgccccca cataacgggg
     2281 tagatcgcac tttatgaggc gatccccctg gtggcggcat gccgtggtgt acgaggtcta
     2341 cgtccgcagt tacgccgacg cgaacggcga cggcgagggc gatctgcgcg gcgtccggtc
     2401 ccggctcggc cacctgtgcg agctcggcgt ggacgcgctg tggctcaccc ccttctaccc
     2461 gtccccgctg gccgacggcg gttacgacgt cgccgactac cgcgacgtcg acccccggtt
     2521 cgggacgctc gacgacttcg acgccctcgt cgcggacgcc cacgagcacg ggttgcggat
     2581 catcgtcgac atcgtcccga accactcctc cgaccggcat ccgtggttcc gcgaggcggt
     2641 cgccgcaccg cccggatcgt ccgcg
//
LOCUS       DASSWR010000360         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1370121, whole genome shotgun sequence.
ACCESSION   DASSWR010000360 DASSWR010000000
VERSION     DASSWR010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370121"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFO47_05970"
     CDS             <1..252
                     /locus_tag="VFO47_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788276.1"
                     /translation="RILEETPTPLNPLGVKGVGEGGSSGCGGAIANAVADALAPLQVD
                     LSELPLHPERLFRRIQEAKARQRGSGGLKPGLPVERGST"
     gene            252..620
                     /locus_tag="VFO47_05975"
     CDS             252..620
                     /locus_tag="VFO47_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788277.1"
                     /translation="MEIKGLVWVGTATERYDATVAFFREMLGLSVFHQSPALTVLVAP
                     GGEWVEVFGPGHEHYGEFDTGPVVEFLVEDLEAARTELEGRGVEFLHENHSWEKFTWA
                     HFRAPDGNIYGITSGPYKTR"
     gene            638..1270
                     /locus_tag="VFO47_05980"
     CDS             638..1270
                     /locus_tag="VFO47_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET8788278.1"
                     /translation="MGEAVERREHDPRPERLVIAMTGATGVIYGVRLLETLRDTSVET
                     HLVMSEWARRTIIAETDRNPDAVRDLADQVHDEGDLSAPLASGSFLTDGMVVAPCSMK
                     SLAAIATGVSESLVHRAADVTLKEGRRLLLLVRETPLSVIHLENMLRVAQAGALVMPP
                     LPAFYARPRSLDEMVDHTVGRVLDHVGVEHDLVRRWGEQRARATRLGVRP"
     gene            complement(1260..1730)
                     /locus_tag="VFO47_05985"
     CDS             complement(1260..1730)
                     /locus_tag="VFO47_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788279.1"
                     /translation="MAKSLGDRLPQTVLRAFDGNDLEAKVGPAHLLVTVDPDGSPRPC
                     MLSAGEVLAPDDRRLRVLLWAGTGTSRNLAAGGPVLLCYVEGGDVVYVKGDPRPLPRE
                     GRGGFERFEIQVRAVETDRHAGLPVTGGITFGVDGGDRAAVARGWERQLAALRA"
     gene            1806..>2191
                     /locus_tag="VFO47_05990"
     CDS             1806..>2191
                     /locus_tag="VFO47_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788280.1"
                     /translation="MRQRAGPALGFATATVGYLAVTTGESLLAPLLPLLREELGLDLR
                     GAGLALGLVSAAIAVGNLGGGALLARRGTRQGTVAGLAVAAAGGLVAAASQGPPSFLL
                     GQALLGLGSGVFFAAGLRAVGVLGGSR"
BASE COUNT          280 a          788 c          831 g          292 t
ORIGIN      
        1 cgcatcctcg aggagacccc gaccccgctc aaccccctcg gcgtcaaggg cgtgggcgag
       61 ggaggcagct cggggtgcgg cggcgccatc gccaacgccg tggccgacgc gctggcgccg
      121 ctccaggtcg acctgagcga gctcccgttg catcccgagc gcttgttccg ccgcatccag
      181 gaagccaagg cacggcaacg ggggtccggg gggctcaagc ccggcctccc ggtcgaaagg
      241 gggtccacct gatggagatc aaggggctgg tctgggtcgg gaccgcgacc gagcggtacg
      301 acgccaccgt cgcgttcttc cgcgagatgc tggggctgtc ggtgttccac cagagcccgg
      361 ccctgacggt gctggtcgcg ccgggcgggg aatgggtgga ggtcttcggg cccggccacg
      421 agcactacgg cgagttcgac accgggccgg tggtggagtt cctggtcgag gacctggagg
      481 cggcccggac cgagctggag ggccgcggcg tcgagttcct gcacgagaac cacagctggg
      541 agaagttcac ctgggcgcat ttccgggcgc cggatggcaa catctacggc atcaccagcg
      601 ggccgtacaa gacgcgctga gcgagcctga gggcgggatg ggggaggcgg tggagcggcg
      661 ggagcatgac ccgcggccgg agcggctggt gatcgccatg accggcgcga ccggcgtgat
      721 ctacggcgtc cggctgctgg agacgctgcg cgacacctcg gtcgagaccc acctggtcat
      781 gagcgaatgg gcgcggcgga ccatcatcgc cgagaccgac cgcaaccccg acgcggtgcg
      841 ggacctggcc gaccaggtcc atgacgaggg cgacctgtcc gcccccctgg ccagcgggtc
      901 cttcctcacc gacggcatgg tggtcgcccc ctgcagcatg aagtccctgg ccgccatcgc
      961 caccggcgtc tccgagagcc tggtccaccg ggccgccgac gtgaccctca aggagggccg
     1021 tcggctgctg ctgctggtgc gcgagacccc gctgtccgtg atccacctgg agaacatgct
     1081 ccgggtcgcc caggccggcg ccctggtgat gccgcccctg ccggcgttct acgcccgtcc
     1141 ccggagcctg gacgagatgg tcgaccacac cgtgggccgg gtcctggacc acgtcggggt
     1201 cgaacacgac ctggtccggc gctggggcga gcagcgcgcc cgggcgacgc ggctcggcgt
     1261 caggccctga gcgcggccag ctggcgctcc cagccccggg ccaccgcggc ccggtcccca
     1321 ccgtcgaccc cgaaggtgat gcccccggtg accggcagcc cggcgtggcg gtcggtctcg
     1381 accgcccgga cctggatctc gaaccgctcg aagccgccac gaccctcgcg gggcagcggt
     1441 cgggggtcgc ccttgacgta gaccacgtcg ccgccctcca cgtagcagag cagcaccggg
     1501 ccgccggcgg ccaggttccg ggaggtgccg gtgcccgccc acagcagcac ccggagccgc
     1561 cggtcgtcgg gggccaggac ctcgccggcg ctcagcatgc acgggcgcgg gctgccgtcg
     1621 gggtccacgg tgaccagcag gtgggcgggc ccgaccttgg cctcgaggtc gttgccgtcg
     1681 aaggcccgca ggaccgtctg gggcagccgg tcgccgagcg acttggccat ggctccgtct
     1741 cctttccagg ttatctctcg acgataagat agttgttcag gacagcggac gtcaggggtg
     1801 gatgcttgag gcagcgggcg gggccggcgc tcggcttcgc cacggccacc gtcggctacc
     1861 tggccgtcac caccggcgag tccctgctcg cgcccctcct gccgctgctg cgcgaggagc
     1921 tcggcctgga cctgcgcggc gccgggctgg cgctggggct ggtctcggcg gcgatcgccg
     1981 tcggcaacct gggcggcggc gccctgctgg cccgccgggg cacacggcag gggacggtcg
     2041 ccgggctggc cgtcgccgcc gccggcgggc tggtcgccgc ggccagccag ggcccgccaa
     2101 gcttcctgct gggccaggcg ctgctcggtc tcggctcggg cgtgttcttc gccgccgggc
     2161 tgcgggcggt cggggtcctc ggcggaagcc g
//
LOCUS       DASSWR010000361         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_917545,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000361 DASSWR010000000
VERSION     DASSWR010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917545"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VFO47_05995"
     CDS             <1..140
                     /locus_tag="VFO47_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018792278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8788281.1"
                     /translation="RRGGLLVGKGGLYGNVLRIAPPLIVTEAEADEGLEILAHALATL
                     G"
     gene            307..>2235
                     /locus_tag="VFO47_06000"
     CDS             307..>2235
                     /locus_tag="VFO47_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET8788282.1"
                     /translation="MQNLAAELEQEQAYVTMLYRRLDTLRERAQAELERALGYDGGGN
                     VAARVDRDAFVARHSGRLAQLAAAEHGLCFGRLDRADGSHLYIGRLGLLDDDREPLLV
                     DWRAPAAQPFYRATWAAPSGVVRRRHLRTRGREVLGIEDDVLDPESLSDDEREGLSGE
                     AALLAALTASRTGRMSDIVATIQAEQDRIIRSDLPGVLVVQGGPGTGKTAVALHRAAY
                     LLYTHRQRLARRGVLVVGPNPTFLRYIEQVLPSLGETDVLLSTVGGLFPGMTATAQEP
                     VEVAAVKGDPRMVGVLAAAVTDRQRVPDADVVVEVGGLRLRLDPATCEQARRTARAAG
                     TPHNQARSVFRRELVQALADQMVAALTRDLPEVDLPDDDGLLVDLFPDERLLDPDLDE
                     IRAELAANPAVRAALDRLWPKLTAQQLVGDLLASPERLAAAGAELDPSERALLLRRPR
                     ADGDPGWTPADVPLLDEAAVLLGDDGARARRAEARRSEQERAELAYAREVLAGTTIGD
                     AARYAPDTSAIDPSLLAGRWQARGPQAGLAERARQDRGWVFGHVIVDEAQELSPMAWR
                     MVMRRSPSRSMTVVGDVAQTGAPWGPGSWAQALDPHAAGRWRVEELTVNYRTPAEIMA
                     VAADVLAAMDAGLAPPRSA"
BASE COUNT          278 a          875 c          828 g          254 t
ORIGIN      
        1 ccaggcgcgg cggcctgctg gtcggcaagg gcggcctgta cggcaacgtc ctgcggatcg
       61 ccccgcccct gatcgtcacc gaggccgagg ccgacgaggg cctggagatc ctcgcccacg
      121 ccctggcaac gctggggtag gaggcgcggc gggcagacgg tgggggctgg tcttggccgc
      181 gaatcgtggc tgacggctgg tcctttgccg cacgcggtgc tacagtcaga attctgcgcg
      241 agcagcctaa actagacgat agaaccgccg catcaacggt agcgcgaacg gaggcacctg
      301 tcaaggatgc agaatctcgc cgctgagctg gagcaggaac aggcctacgt gacgatgctg
      361 taccggcgcc tggacacgct gcgcgagcgc gcccaggccg agctggagcg ggccctgggc
      421 tacgacggcg gcggcaacgt cgcggcccgg gtggaccggg acgcgttcgt ggccaggcac
      481 agcgggcgcc tggcccagct ggccgcggcc gagcacggcc tctgcttcgg gcggctggac
      541 cgggccgacg gcagccacct gtacatcggc cgcctgggcc tgctcgacga cgaccgcgaa
      601 ccgctgctgg tcgactggcg cgcccccgcg gcccagccgt tctaccgggc cacctgggcc
      661 gccccgagcg gcgtggtccg ccgccgccac ctgcggaccc gcggccggga ggtcctgggg
      721 atcgaggacg acgtgctcga cccggagtcg ctcagcgacg acgagcgcga gggcctcagc
      781 ggcgaggcgg ccctgctggc cgccctcacg gccagccgca ccggccgcat gtccgacatc
      841 gtggccacca tccaggccga gcaggaccgg atcatccgct ccgacctccc cggcgtcctg
      901 gtcgtgcagg gcgggccggg caccggcaag accgccgtgg ccctgcaccg ggccgcctac
      961 ctgctctaca cccaccgcca gcgcctggcc cggcggggcg tgctggtggt cgggcccaac
     1021 ccgaccttcc tgcgctacat cgagcaggtg ctgccgtcgc tgggggagac cgacgtgctg
     1081 ctgtccaccg tcggcgggct gttcccgggc atgaccgcga ccgcccagga gccggtcgag
     1141 gtggccgccg tcaagggcga cccgcgcatg gtcggcgtgc tggccgcggc cgtcaccgac
     1201 cgccagcgcg tccccgacgc cgacgtcgtg gtcgaggtgg gcgggctgcg gctgcggctg
     1261 gacccggcca cctgcgagca ggcgcggcgc accgccagag ccgccggcac cccgcacaac
     1321 caggcccgct cggtgttccg gcgcgagctg gtgcaggccc tggccgacca gatggtggcc
     1381 gccctcaccc gcgacctgcc cgaggtcgac ctgcccgacg acgacggcct gctggtcgac
     1441 ctgttccccg acgagcggct gctcgacccc gacctggacg agatccgggc cgagctggcc
     1501 gccaacccgg ccgtgcgcgc cgccctcgac cggctgtggc cgaagctgac cgcccagcag
     1561 ctcgtcggcg acctgctcgc ctcccccgag cgcctggccg cggccggcgc cgagctcgac
     1621 ccgtccgagc gggccctgct gctgcgccgc ccccgggcgg acggcgaccc cggctggacc
     1681 ccggccgacg tgcccctgct cgacgaggcg gccgtgctgc tgggcgacga cggcgcccgg
     1741 gcgcgccggg ccgaggcccg ccgctccgag caggagcggg ccgagctcgc ctacgcccgc
     1801 gaggtcctgg ccggcaccac catcggggac gccgcccgct acgcccccga caccagcgcc
     1861 atcgacccgt cgctgctggc cggccgctgg caggcgcggg ggccacaggc cggcctggcc
     1921 gagcgggccc gccaggaccg cggctgggtg ttcggccacg tgatcgtgga cgaggcccag
     1981 gagctgtcgc cgatggcctg gcgcatggtg atgcggcgca gccccagccg gtcgatgacc
     2041 gtggtcggcg acgtcgccca gaccggcgcc ccctggggcc ccggctcgtg ggcccaggcg
     2101 ctggacccgc acgccgccgg ccgctggcgg gtcgaggagc tgacggtcaa ctaccgcacc
     2161 ccggccgaga tcatggccgt tgccgccgac gtgctggccg ccatggacgc cggcctggcc
     2221 ccgccccgct cggcc
//
LOCUS       DASSWR010000362         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_183717,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000362 DASSWR010000000
VERSION     DASSWR010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183717"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="VFO47_06005"
     CDS             <1..1667
                     /locus_tag="VFO47_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788283.1"
                     /translation="WAASIAYGLIIAALLAHDQALLPLLIVPIGLTFLGNRAWARSLA
                     QGQRMHSLYAAGRALSNRLGDTRAWEVFARQVADVLNCEGAAVFLGRDHEGALDVIST
                     GGSERLSVPPSKAAWEQAAKGWATRAGWPRYTMVPMEADGQVVGFAAAFGPRVDADFT
                     GDDTETLLTLANQGAAALVNEELYRTAESERAQLLDIVEHSSDGIYTVGPDRTVKSWN
                     AAMVALTGYQEDEAVGQKCFNLLRARDGDGVDMCAADCPILAAAASGHEEVRDASVLN
                     KEGLARWIRYAHAPIVAADGDMDADVVVVRDVTRERQTDELKTDFVASVSHELRTPLT
                     PIKGFLMTLLREDRDFAQDRRREYYKLMLMQSQRLERLIEDLLEVTRLEAGAGLVDTT
                     AIDAVDLVRQVVDRFTSEDPDRTVHVAAPDHAVYCRGDWMRIDQVLGNLLSNALRYAP
                     PHEPVEVRLVPQGREVVFEVRDWGPGIPIDEQSRIFERFHRVGHYMTREPGGAGLGLY
                     LAKRLVEAMGGRIWVSSRLGHGSVFSFALPAEPSLDAVAPRDRHIG"
     gene            1884..3173
                     /locus_tag="VFO47_06010"
                     /pseudo
     CDS             1884..3173
                     /locus_tag="VFO47_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            3569..>3984
                     /locus_tag="VFO47_06015"
     CDS             3569..>3984
                     /locus_tag="VFO47_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET8788284.1"
                     /translation="MNRLLLQRGLAAVYLVAFLVTANQFRPLLGERGLLPAPRFLAEV
                     PFGRAPSLFHLRYSDRLLTLVAWTGAGLAAAALTGLSEAGPAWMSVAVWLAMWALYLS
                     IVNVGQTFYGFGWETLLLEAGFLAVLLGPAGTAPPTL"
BASE COUNT          530 a         1574 c         1354 g          526 t
ORIGIN      
        1 actgggccgc cagcatcgcc tacggcctga tcatcgccgc cctcctggcc cacgaccagg
       61 cgctgctgcc gctgctgatc gtccccatcg gcctgacctt cctcggcaac cgggcctggg
      121 cccgcagcct ggcccagggc cagcgcatgc actcgctgta cgcggccggg cgggccctgt
      181 ccaaccgcct cggcgacacc cgcgcctggg aggtcttcgc ccgccaggtc gccgacgtcc
      241 tcaactgcga gggggcggcg gtgttcctcg gccgcgacca cgagggcgcc ctcgacgtga
      301 tctccaccgg cggcagcgag cggctgagcg tgccgccgtc caaggccgcc tgggagcagg
      361 ccgccaaggg ctgggcgacc cgcgccggct ggccccgcta caccatggtc ccgatggagg
      421 ccgacggcca ggtggtcggg ttcgccgccg ccttcggccc ccgggtcgac gccgacttca
      481 ccggcgacga caccgagacc ctgctcaccc tggccaacca gggggcggcg gccctggtca
      541 acgaggagct gtaccgcacc gccgagagcg agcgggccca gctgctggac atcgtcgagc
      601 actcctcgga cggcatctac accgtcgggc cggaccgcac cgtcaagtcc tggaacgcgg
      661 ccatggtcgc cctcaccggc taccaggagg acgaggcggt ggggcagaag tgcttcaacc
      721 tgctccgggc ccgcgacggc gacggggtcg acatgtgcgc cgccgactgc ccgatcctgg
      781 ccgcggccgc ctccggccac gaggaggtcc gcgacgcctc ggtgctgaac aaggaggggc
      841 tggcccgctg gatccgctac gcccacgccc ccatcgtggc cgccgacggc gacatggacg
      901 ccgacgtggt ggtggtccgc gacgtcaccc gcgagcgcca gaccgacgag ctcaagaccg
      961 acttcgtggc cagcgtctcc cacgagctgc ggaccccgct cacgcccatc aagggcttcc
     1021 tgatgaccct gctgcgcgag gaccgcgact tcgcccagga ccgccggcgc gagtactaca
     1081 agctgatgct gatgcagtcc cagcggctgg aacggctgat cgaggacctg ctggaggtga
     1141 cccggctgga ggccggggcc gggctggtcg acaccaccgc gatcgacgcc gtcgacctgg
     1201 tccgccaggt cgttgaccgc ttcaccagcg aggacccgga ccgcacggtc cacgtggccg
     1261 cccccgacca cgccgtctac tgccgcggcg actggatgcg gatcgaccag gtgctgggca
     1321 acctgctctc caacgccctg cgctacgccc cgccccacga gccggtcgag gtccgcctcg
     1381 tcccccaggg ccgcgaggtc gtgttcgagg tccgcgactg gggcccgggc atccccatcg
     1441 acgagcagtc ccgcatcttc gagcgcttcc accgggtcgg ccactacatg acccgcgagc
     1501 cgggcggcgc cggcctcggc ctctatctgg ccaagcgcct ggtcgaggcc atgggcggcc
     1561 gcatctgggt ctccagccgc ctcggccacg gctcggtctt ctccttcgcc ctccccgccg
     1621 agcccagcct cgacgccgtc gcccctcgcg accgccacat cggctgaggc gggacgagcg
     1681 tcagcgccac cgcctggccc tgacggcccc gacccgcccg gaggcttggc ccggcctgtc
     1741 ccagtcgcgg ctgggcaggt ggcggcgttt ccggcggtgc ggggcagcag ggctggcacc
     1801 ggtatcctct tgctgctgcg cgccgcccgt tcgggggtgg cgctcggacg caaggaggcg
     1861 ttttgcagcg acgggtgatc atcatgggag ccgccggccg ggacttccac aacttcaaca
     1921 gcgccttccg gggcgacccg gccacgacgg tggtggcgtt caccgccacc cagatcccct
     1981 tcatcgacga ccggacctac ccggccgccc tggccgggcc cgcctatccg cgggggatcc
     2041 cgatcgtccc cgaggagcgc ctcgacgacc tgatcgccgc cgaggacgtg gacgaggtgg
     2101 tgttcagtta ctccgacgtc cgccacgtcg acgtgatgca cgtcgcctcc cgggtcctgg
     2161 ccgccggggc cgacttccgc ctgctcggcc gcaggcagac cgagctggtg gcggccgtgc
     2221 cggtggtggc ggtgtgcgcg gtccgcaccg gggccggcaa gtcgcagacc acccggaggg
     2281 tggccgagat cctccgggcc gccggccgca aggtcgtggc cgtccgccac cccatgccct
     2341 acggcgacct ggccagccag gccgtgcagc gcttcacgac cgtcgaggac ctggtggccg
     2401 cccgcgccac catcgaggag cgcgaggagt acgagccgca cctggccgcc gggacggtcg
     2461 tgtacgccgg cgtcgactac cacgccatcc tggagcgcgc ccaggccgag gccgacgtgc
     2521 tgctgtggga cggcggcaac aacgacctgc cgttcttccg gcccgacctg cacatcgtgg
     2581 tcgccgaccc gctgcgggcc gggcacgagt cgagctacca ccccggcgag gccaacgccc
     2641 gggcggccga cgtgctcgtc atcaacaagg tcgacgaggc cacccccgag caggtcgcgg
     2701 acctggagga gcagctcaag cagctcaacg ccggggccgt ggtcgtcaag gcggccagcc
     2761 cggtccgggt cgaggacccc gagctggtca ccggcaggcg ggtcctggtc gtcgaggacg
     2821 ggccgaccct gacccacggg gggatgagct acggcgccgg cgtggtcgcc gcccgccgcc
     2881 tcggcgccgc cgagatcgtc gacccccgcc ccttcctggt cggctccctg gccgaggtgt
     2941 acgagcgcta cccgcacatc ggcgccctgg tcccggccat gggctactcc gacgcccaga
     3001 tccgcgagct ggagacggtc atcaaccagg ccccggtgga cgcggtggtg atcggcaccc
     3061 cgatcgacct gcgggccgtc gccgacatcg cccacccagc cacccgggtc ggctacgacc
     3121 tggtcgagca gggccacccc gacctggccg ccgtcctcga ccccgtgatc cgcgccagcg
     3181 ccacccctgc ccccgccccc cgccgaggag ccgccccgat gaccaccgac gcccagaccg
     3241 acgactaccg cgccttcctg gaggcccagc ccgtgcccac cctgcgcgac tgggccatgg
     3301 aggtcgccaa ggacaagcac gacgtcggct tctactggga cgtggccaag cacctgcccg
     3361 ccaccgagga cgccaacctg gactgggccg cctacgaccc gatcgacgcc atccgcgagc
     3421 tcggccacct ggtcacccac ttccgcgagg aggccgccga ccccgaggtc gccgacctgc
     3481 tccgcatccg ctacgtcgac tacctggtcg agcacgccga cgaccggagg ttcgaccgac
     3541 ggactggctc cagtcacccg acgactggct gaaccgcctc ctcctgcagc gggggctggc
     3601 cgccgtctac ctggtcgcct tcctggtcac ggccaaccag ttccgcccgc tgctggggga
     3661 gcgcgggctg ctgccggccc cccggttcct ggccgaggtc cccttcggcc gggcgccgag
     3721 cctcttccac ctgcgctact ccgaccggct cctgacgctg gtcgcctgga ccggggccgg
     3781 gctggccgcc gccgccctca ccggcctgtc cgaggcgggg ccggcctgga tgtcggtggc
     3841 cgtctggctg gccatgtggg ccctgtacct gtcgatcgtc aacgtcggcc agaccttcta
     3901 cgggttcggc tgggagacgc tgctgctgga ggcgggcttc ctggccgtgc tcctcgggcc
     3961 ggccggcacg gccccgccca ccct
//
LOCUS       DASSWR010000363         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_268269,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000363 DASSWR010000000
VERSION     DASSWR010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268269"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFO47_06020"
     CDS             complement(<1..509)
                     /locus_tag="VFO47_06020"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET8788285.1"
                     /translation="MAEPAPIDPAGAGAGMPRREPGARAGRNLPLAIVTALLLAGVLI
                     GSLVLSRPAFIAFLLGIVTLALLELLTVLRARATRPAGPVVLAMGALLVAGAYLEGPS
                     ALSFGLLVTILAAFGWYLVDRGRTEVTRNVAATIFAVVYVPFMAAHLSLVVGRADHYV
                     GALIGYAALV"
     gene            complement(516..1073)
                     /gene="frr"
                     /locus_tag="VFO47_06025"
     CDS             complement(516..1073)
                     /gene="frr"
                     /locus_tag="VFO47_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET8788286.1"
                     /translation="MIEDTLRQAETKMSKAIDVAKEEFSGIRTGRANPALVNRLQVGY
                     YGTPTPLQQLAGISAPEARLLVVSPYDRNAAAAIEKAIRNSDLGINPASDGQVIRLAF
                     PQLTEERRKEYVKLAKHRAEEARTSIRNVRRHEKDALERLERDGEISQDELRRAEQRL
                     QGITDKYVAEVDTLLQHKERELLEV"
     gene            complement(1070..1873)
                     /gene="pyrH"
                     /locus_tag="VFO47_06030"
     CDS             complement(1070..1873)
                     /gene="pyrH"
                     /locus_tag="VFO47_06030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009328866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET8788287.1"
                     /translation="MVGDQPEPVDSGEGKPRFERVVVKLSGEAFADTSVGFGLDYGKI
                     DGVARQLVEVLDLGVQVAVVVGGGNIVRGSAAEAAGMDRARADYMGMLGTVMNCLALQ
                     DALERRQVVTRVQTAIQMTQIAEPYVPRRAVRHLEKGRIVIFGAGMGAPFFSTDTTAA
                     QRSLEIGAEVILMAKNGVDGVYDADPKKHPEARKFLWLDYLEAIRRELRVMDTTALSL
                     CKDHDLPIIVFDLLTEGNIRRAVCGEPIGTVVGPNDVVEADETQRGEPR"
     gene            complement(1932..2540)
                     /locus_tag="VFO47_06035"
     CDS             complement(1932..2540)
                     /locus_tag="VFO47_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET8788288.1"
                     /translation="MEITTAQVKALRDATGAGMMDCRNALREAGGDLDKATKLLREAG
                     KAGVEKRAGRSTTQGVVDAYLHTPDPNLPPKLGVLVELDCETDFVAKTDEFQRLAHEI
                     ALHIAVFDPTYVRREDVPEHVIEKEKEIYASQAEGKPEHIVERIVEGKLKDYYKQVVL
                     LDQPYIRDDKQSIKDLLDTYSAKVREKLVLRRFSRFKVGEGA"
     gene            complement(2614..>2920)
                     /locus_tag="VFO47_06040"
     CDS             complement(2614..>2920)
                     /locus_tag="VFO47_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788289.1"
                     /translation="EAAAEPTKAETEAAAETEAEAEGGTTQPEAAAEPTTADVEIPED
                     ATPEGATIEPEAGTEAAEAAEAGTGSEAEGETQAEATPAEAAEEAADPTNETRSPRV"
BASE COUNT          343 a         1053 c         1041 g          483 t
ORIGIN      
        1 accagcgccg cgtagccgat cagggcgccg acgtagtggt cggcccggcc caccaccagc
       61 gacaggtggg cggccatgaa cggcacgtag acgacggcga agatggtggc ggcgacgttg
      121 cgggtgacct cggtgcgccc ccggtcgacc aggtaccagc cgaacgcggc caggatggtc
      181 accagcagcc cgaacgacag cgccgacggc ccctccaggt aggccccggc gaccagcagc
      241 gcccccatgg ccaggacgac cggcccggcc gggcgggtgg cccgcgcccg cagcacggtc
      301 agcagctcca gcagggccag ggtgacgatc ccgagcagga aggcgatgaa cgccggccgc
      361 gacaggacca gcgagccgat gaggacgccg gccaggagca gcgcggtgac gatggccagc
      421 ggcaggttgc gcccggcccg ggcgcccggc tcgcgccgcg gcatccccgc cccggctccg
      481 gcggggtcga tcggggccgg ctcagccacc gacgctcaga cctcgagcag ctcccgctcc
      541 ttgtgctgga gcagggtgtc gacctcggcc acgtacttgt cggtgatgcc ctggaggcgc
      601 tgctcggccc ggcgcagctc gtcctgggag atctcgccgt cgcgctccag gcgctccagc
      661 gcgtccttct cgtgccggcg gacgttgcgg atgctggtcc tggcctcctc cgcccggtgc
      721 ttggccagct tcacgtactc cttgcggcgc tcctcggtga gctgcgggaa ggcgaggcgg
      781 atgacctggc cgtcgctggc cgggttgatg ccaaggtcgg agttgcggat ggccttctcg
      841 atggccgcgg cggcgttgcg gtcgtagggg gagaccacca gcagccgggc ctccggggcg
      901 gagatgccgg ccagctgctg cagcggggtg ggggtgccgt agtagccgac ctggaggcgg
      961 ttgaccaggg ccgggttggc gcggccggtg cggatcccgc tgaactcctc cttggccacg
     1021 tcgatcgcct tgctcatctt cgtctctgcc tgccggagcg tgtcctcgat caccttggct
     1081 cccctcgctg cgtctcgtct gcctcgacga cgtcgttcgg gccgaccacc gtgccgatcg
     1141 gctcgccgca caccgcccgc cggatattgc cctcggtgag caggtcgaac acgatgatgg
     1201 gcaggtcgtg atccttgcac aacgacaggg cggtggtgtc catcacccgc agctcgcgcc
     1261 ggatcgcctc caggtagtcg agccagagga acttgcgcgc ctccgggtgc ttcttggggt
     1321 cggcgtcgta gaccccgtcg acgccgttct tggccatgag gatgacctcg gcgccgatct
     1381 cgagcgaccg ctgggcggcg gtggtgtcgg tcgagaagaa gggggcgccc atgcccgccc
     1441 cgaagatcac gatgcgcccc ttctccaggt ggcgcacggc ccgccgcggg acgtacggct
     1501 ccgcgatctg ggtcatctgg atggccgtct gcacccgggt cacgacctgg cggcgctcca
     1561 gggcgtcctg cagggccagg cagttcatga ccgtccccag catgcccatg tagtcggccc
     1621 tggcccggtc catcccggcc gcctcggcgg ccgacccgcg gacgatgttg cctccgccga
     1681 ccacgaccgc gacctgcacg cccaggtcga ggacctcgac gagctgcctg gccacgccgt
     1741 cgatcttgcc gtagtcgagc ccgaacccga cgctggtgtc ggcgaacgcc tcccccgaca
     1801 gcttcaccac gacccgctcg aaccggggct tgccctcccc gctgtcgacc ggctccggct
     1861 ggtcccccac catccgatat gccatggctt cgctcctccg cccgcccgac cgatcggctc
     1921 ccaggaacgg gtcaggcgcc ttccccgacc ttgaaacggg agaagcggcg caggaccagc
     1981 ttctcgcgga ccttggccga gtaggtgtcg agcaggtcct tgatggactg cttgtcgtcg
     2041 cggatgtacg gctggtcgag cagcacgacc tgcttgtagt agtccttgag cttgccctcg
     2101 acgatcctct cgacgatgtg ctcgggcttg ccctcggcct ggctggcgta gatctccttc
     2161 tccttctcga tgacgtgctc gggcacgtcc tcgcgccgca cgtaggtcgg gtcgaagacg
     2221 gcgatgtgga gggcgatctc gtgggccagg cgctggaact cgtcggtctt ggccacgaag
     2281 tcggtctcgc agtcgagctc gacgagcacg cccagcttgg gcggcaggtt cgggtcgggg
     2341 gtgtgcaggt aggcgtcgac cacgccctgg gtggtgctgc ggccggcccg cttctcgacg
     2401 ccggccttgc cggcctcgcg cagcagcttg gtggccttgt ccaggtcgcc gcccgcctca
     2461 cgcagcgcgt tcctgcagtc catcattccg gccccggtgg cgtcccggag ggccttgacc
     2521 tgagcggtcg tgatctccat gtcgggtcgt gctcccttgc tgcgctgtcg atgctcggtc
     2581 ggtgtcgggt gggctcccgg cccgggcggt ccgctagacc cggggcgaac gggtctcgtt
     2641 ggtcgggtcg gccgcctcct cggcggcctc cgccggggtg gcctcggcct gggtctcccc
     2701 ctcggcctcc gacccggtcc cggcctcggc ggcctcggcg gcctcggttc cggcctcggg
     2761 ctcgatggtc gcgccctcgg gcgtggcgtc ctcggggatc tcgacgtcgg ccgtggtcgg
     2821 ctcggcggcc gcctcgggct gggtggttcc gccctcggcc tcggcctcgg tctcggcggc
     2881 ggcctcggtc tcggccttgg tgggctcggc ggcggcctcg
//
LOCUS       DASSWR010000364         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1003356, whole genome shotgun sequence.
ACCESSION   DASSWR010000364 DASSWR010000000
VERSION     DASSWR010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003356"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFO47_06045"
     CDS             <1..833
                     /locus_tag="VFO47_06045"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8788290.1"
                     /translation="PGQPPAPHDLWGAWNLDPVLLGGFLLAAWAYWRGQTSGPRRPID
                     TWRARCFTGAVVALGLALLSPLDALSGALASANMVQHLLLLLVAAPLLALSAPASAIL
                     RGSPLALRRASGRWRRRLGLTHGNLGVLRHPAAVWLLSVGAIWFWHAAAPYDATLDNQ
                     LLHVLEHASFLVTAVLFWHVVIGVRGVARVSGGLGVLLVFAMAMQSVFLAVLLTFART
                     PWYSGYATTTASWGLDPLADQQVAGVIMWIPAGGIYLAAALVLLVTWIRATEREDVMV
                     "
     gene            906..>2535
                     /locus_tag="VFO47_06050"
     CDS             906..>2535
                     /locus_tag="VFO47_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8788291.1"
                     /translation="MAKRTVADQFVEILLAAGVRRMYGVVGDSLNPVVDAIRRHDGID
                     WVQVRHEEAGAFAAGAEAQLTGRLVACAGSCGPGNLHLINGLYDAHRSMAPVLALAAQ
                     IPSTEIGTGYFQETRPTLTFQECSHYCELVTRPEQLPRVTQIAVQQALGRGGVGVVAL
                     PGDVADQEASGAYLEHTYRLDQRPAARPSDEVLDEFVGLVEGAGRVTLFCGRGVAGAH
                     QEVLALAERLKAPVGHALGGKEWIQHDNPYDVGMSGLLGYGACYEAMHGCDLLVLLGT
                     DFPYVQFMPTRPKVVQVDIRRERLGSRSRLDLGVWGDVKETLRALLSRVPERSDRAFL
                     DQMLARHRELAGRVRAYTDEVRGRRPIHPEHVTAVLDDLAADDAVFTVDTGMCNVWAA
                     RLIRATAGRRMLGSFAHGSMANALPQAIGAQLPDRGRQVIAMCGDGGFAMLMGELLTL
                     LQYDLPVKLVVFDNGSLGMVALEMLVAGYPPYGTGLKNPDFAAMARAAGMTGIRIEDP
                     GDVQDGLREALAADGPVLVDVLTDPNALSMPPKIT"
BASE COUNT          312 a          925 c          911 g          387 t
ORIGIN      
        1 accccggcca gcctccggcg cctcatgacc tctggggcgc ctggaacctg gacccggtcc
       61 ttctgggtgg cttcctgctc gccgcctggg cctactggcg cggccagacc agcgggccgc
      121 ggcggccgat cgacacctgg cgcgccaggt gcttcaccgg ggccgtggtg gcgctcggcc
      181 tggcgttgct gtcacccctg gacgccctgt cgggcgcgct cgcctcggcc aacatggtcc
      241 agcacctgct gctgctgctg gtcgcggcgc cgctgctggc cctcagcgcc ccggccagcg
      301 ccatcctgcg cggcagccct ctggccctgc gccgggccag cggccgctgg cggcggcggc
      361 tcgggctgac ccacggcaac ctcggcgtcc tgcgccaccc ggccgccgtg tggctgctct
      421 cggttggggc catctggttc tggcacgccg cggcccccta cgacgccacc ctggacaacc
      481 agctcctcca cgtcctggag catgccagct tcctggtcac ggccgtcctg ttctggcacg
      541 tcgtcatcgg tgtccgtggc gtggcccggg tctcgggcgg ccttggcgtc ctgctcgtgt
      601 tcgccatggc gatgcagagc gtgttcctcg cggtcctgct gaccttcgcc cggacaccgt
      661 ggtactcggg ctacgccacg acgaccgcct cctggggcct ggacccgctc gccgaccagc
      721 aggtggccgg ggtcatcatg tggatcccag ccggcggcat ctacctcgcg gcggcgctgg
      781 tcctcctggt cacctggatc cgagccaccg aacgcgagga cgtcatggtc taggcgacag
      841 gatgcgtgcg agtctttcct gcagcgccca gcccgaccgg gggctgtcgg accgtggagg
      901 tcgtcatggc caagcgcacc gtcgccgacc agttcgtcga gatcctgctc gccgccgggg
      961 tgcggcgcat gtacggggtg gtgggcgaca gcctcaaccc ggtcgtggac gccatccgcc
     1021 gccacgacgg catcgactgg gtgcaggtgc gacacgagga ggccggcgcg ttcgcggctg
     1081 gcgccgaggc gcagctgacc ggtcggctgg tggcctgcgc gggcagctgc gggcccggga
     1141 acctgcacct gatcaacggc ctgtacgacg cccatcggag catggcgccg gtgctggcgc
     1201 tggccgcgca gatcccgagc accgagatcg gcaccggcta cttccaggag acgcgtccca
     1261 ccctgacgtt ccaggagtgc agccactact gcgagctcgt cacccgtccc gagcagctgc
     1321 cgcgggtcac ccagatcgcg gtccagcagg cgctcggccg cggcggggtg ggggtcgtcg
     1381 ccctgcccgg agacgtggcc gaccaggagg ccagcggcgc ctatctggag cacacctacc
     1441 ggttggatca gcggcccgcc gcccggccgt ccgacgaggt gctcgacgag ttcgtcgggc
     1501 tggtcgaagg ggcggggcgg gtgacgctgt tctgcggccg cggggtcgcc ggcgcccacc
     1561 aggaggtcct ggccctggcc gagcggctca aggccccggt cgggcacgcg ctcggcggca
     1621 aggagtggat tcagcacgac aacccgtacg acgtcggcat gtccgggctg ctcggctacg
     1681 gggcctgcta cgaggccatg cacggatgcg acctgctggt gctgctgggc accgacttcc
     1741 cctatgtgca gttcatgccg acccggccca aggtggtgca ggtcgacatc cggcgcgagc
     1801 ggctgggcag ccggtcgcgc ctggacctgg gtgtctgggg tgatgtcaag gagaccctga
     1861 gggcgctgct gtcccgggtg cccgagcggt cggaccgcgc cttcctggac cagatgctgg
     1921 cccggcatcg tgagctggcg gggcgggtcc gggcctatac cgacgaggtg cgcgggcgcc
     1981 ggcccatcca ccccgagcac gtcaccgccg tgctcgacga cctggccgcc gacgacgccg
     2041 tgttcaccgt cgacaccggc atgtgcaacg tctgggcggc gcgcctgatc cgcgccaccg
     2101 ccgggcggcg catgctcggc tcattcgcgc atgggtcgat ggccaacgcg ctgccgcagg
     2161 cgatcggcgc ccagctgccc gaccgtggcc gccaggtgat cgccatgtgc ggcgacggcg
     2221 gcttcgccat gctcatgggt gagctgctca ccctcctcca gtacgacctg ccggtgaagc
     2281 tcgtcgtctt cgacaacggc tcgctgggca tggtggcgct ggagatgctg gtcgccggct
     2341 acccgcccta cgggaccggc ttgaagaacc cggacttcgc cgccatggcc cgggccgccg
     2401 gcatgaccgg gatccgcatc gaggacccag gcgacgtcca ggatgggctg cgcgaggcac
     2461 tggcggccga cgggccggtg ctggtggacg tgcttaccga ccccaacgcc ctgtcgatgc
     2521 cgcccaagat cacgg
//
LOCUS       DASSWR010000365         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_664203,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000365 DASSWR010000000
VERSION     DASSWR010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664203"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /gene="acs"
                     /locus_tag="VFO47_06055"
     CDS             complement(<1..1784)
                     /gene="acs"
                     /locus_tag="VFO47_06055"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET8788292.1"
                     /translation="MAESSPAIENLFSEGRSFPPPDDFKADALVTDQSLHERAARDLE
                     GFWAEEAERLRWMKPWDQVLEWNLPFAKWFVGGQLNVSDNCLDRHVEAGGGDKVAYHW
                     EGEPGDTRTITYGELRDDVARFANALKSIGVRRGDRVNIYMGMVPELPVAMLACARLG
                     AAHSVVFGGFSADALRDRINDAEAKVLITQDEGWRAGKVVPLKQNADDAVADTPSIEK
                     VVVLRRTGNDVPWTEGRDVWWHDLAEGQSTDCPPEAMDAEDLLYLLYTSGTTAKPKGI
                     MHTTGGYLTGVATTHRLVFDLHPDTDVYWCAADIGWVTGHSYIVYAPLANHATSILYE
                     GAPNFPDKDRWWEIIEKYKVTILYTAPTAIRTFMKWGTDWPEKHDLSSLRLLGSVGEP
                     INPEAWVWYQTNIGGGRCPVVDTWWQTETGMIMITPLPGAIPTKPGSATFPFPGVDAD
                     IVDNDGNSVGIPGGGYLVLQKPWPAMLRGIYGDPERYKETYWSRFEGRYFAGDGAKRD
                     EDGYFWLLGRIDDVMNISGHRISTTEVESALVDHHDVAEAAVCGKVDQTTGQQIVAFV
                     TLKGNVEPTDDTTEVLREHVARKIGKLAR"
     gene            2114..>2503
                     /locus_tag="VFO47_06060"
     CDS             2114..>2503
                     /locus_tag="VFO47_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788293.1"
                     /translation="MALVPVPSMPPLAVIAHDDPGTADSLRHAVEAVAGWQVLMADPS
                     PAGLAAAVAAGPSVALVGCDALAHLPADCQTPLVAIGDDDRPADVRAALAAGARSLLT
                     WPDGVADLPGELARVAAFARPGPAGGPT"
BASE COUNT          306 a          950 c          839 g          408 t
ORIGIN      
        1 cgggccagct tcccgatctt ccgggccacg tgctcgcgca gcacctcggt ggtgtcgtcg
       61 gtcggctcaa cattcccctt gagcgtcacg aacgcgacga tctgctgccc ggtggtctgg
      121 tcgaccttgc cgcacaccgc cgcctcggcg acgtcgtggt ggtcgaccag ggccgactcc
      181 acctcggtgg tggagatccg gtggcccgag atgttcatca cgtcgtcgat ccgcccgagc
      241 agccagaagt agccgtcctc gtcccgcttg gccccgtcgc cggcgaagta cctgccctcg
      301 aaccggctcc agtaggtctc cttgtaccgc tcggggtcgc cgtagatccc gcgcagcatc
      361 gccggccacg gcttctggag caccaggtat ccaccacccg ggatgccgac cgagttgccg
      421 tcgttgtcga cgatgtcggc atccactccc gggaacggga aggtcgccga ccccggcttg
      481 gtggggatgg cgcccggcag cggggtgatc atgatcatgc ccgtctcggt ctgccaccag
      541 gtgtcgacca ccgggcagcg cccgccgccg atgttggtct ggtaccagac ccaggcctcg
      601 gggttgatcg gctcaccgac gctgccgagc agccgcaggc tggagaggtc gtgcttctcg
      661 ggccagtcgg tgccccactt catgaaggtg cggatggccg tcggcgccgt gtagaggatc
      721 gtgaccttgt acttctcgat gatctcccac cagcggtcct tgtcggggaa gttgggcgcg
      781 ccctcgtaga ggatgctggt ggcgtggttg gccagcgggg cgtagacgat gtaggagtgg
      841 ccggtgaccc agccgatgtc ggccgcgcac cagtacacgt cggtgtcggg atgcaggtcg
      901 aacaccagcc ggtgggtggt cgccaccccg gtcaggtagc cgccggtggt gtgcatgatc
      961 cccttgggct tggccgtggt ccccgaggtg tagagcaggt agagcaggtc ctcggcgtcc
     1021 atcgcctcgg gtgggcagtc ggtcgactgc ccctccgcca ggtcgtgcca ccagacgtcg
     1081 cggccctcgg tccaggggac gtcgttgccg gtccgccgca gcaccacgac cttctcgatg
     1141 ctgggcgtgt cggcgaccgc gtcgtcggcg ttctgcttga gcggcaccac cttgccggcc
     1201 cgccacccct cgtcctgggt gatcaggacc ttggcctcgg cgtcgttgat ccggtcccgc
     1261 agcgcgtcgg cggagaaccc gccgaacacc accgagtggg ccgcgcccag ccgggcgcag
     1321 gcgagcatcg ccaccggcag ctcggggacc atgcccatgt agatgttgac ccggtcgccc
     1381 cgccgcacgc cgatcgactt gagcgcgttg gcgaaccggg cgacgtcgtc gcgcagctcc
     1441 ccgtaggtga tggtccgggt gtcgcccggc tcaccctccc agtggtaggc gaccttgtcg
     1501 ccgccgccgg cctcgacgtg gcggtcgagg cagttgtccg agacgttcag ctgcccgccg
     1561 acgaaccact tggcgaaggg cagattccac tcgagcacct ggtcccatgg cttcatccag
     1621 cgcagccgtt cggcctcctc ggcccagaac ccctccaggt cccgtgccgc ccgctcgtgc
     1681 agtgactggt cggtgaccag cgcgtcggcc ttgaaatcgt ccggcggggg gaaggaccgc
     1741 ccctcggaga agaggttttc gatcgcggga ctgctctcgg ccatgcaatc cgcctccctg
     1801 cggcagttcg tgttccccgg aagcttactc ggcccaccgg gtcccgaaac gcgcgtatcc
     1861 acacccgtgc acggcccggt cccgccgctg gtcccggtct cgccgctggc cctggcgtat
     1921 cccagttgcc cggtcccggc gttccggcgg ccctccccgt cgccgccccg ccgggaacct
     1981 gcgggagtta tgcacattct cgcgggagcg tcttccccct ggtgtcccgc ccggccatcc
     2041 ttcccggcgc cggaccggcc gagcgccgca tccggcccgc cgagcccgaa ccccaggcac
     2101 cgggaggatg cccgtggccc tcgtccccgt cccgtcgatg cccccgctgg ccgtcatcgc
     2161 ccacgacgac cccggcaccg ccgactccct gcggcacgcg gtcgaggccg tcgccggctg
     2221 gcaggtcctc atggccgacc cgagccccgc cggcctggcc gcggccgtcg ccgcggggcc
     2281 ctcggtcgcc ctggtcggtt gcgacgcgct tgcccacctg cccgccgact gccagacccc
     2341 attggtcgcc atcggcgacg acgaccgccc ggccgacgtc cgggcggcgc tcgccgcggg
     2401 cgcccgcagc ctgctcacct ggcccgacgg ggtcgccgac ctgccaggtg agctcgcccg
     2461 ggtcgccgcc ttcgcccggc ccggtcccgc cggcggcccc acc
//
LOCUS       DASSWR010000366         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_71048,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000366 DASSWR010000000
VERSION     DASSWR010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71048"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFO47_06065"
     CDS             complement(<1..270)
                     /locus_tag="VFO47_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788294.1"
                     /translation="MKTPQPKPNKLHDELDALLDGRPVELTDELAPLVEAADALRAEL
                     ATFQLDPEVADRHQERALERSGTVLELPVRRQPSGWDMRRRVVAVA"
     gene            complement(267..902)
                     /locus_tag="VFO47_06070"
     CDS             complement(267..902)
                     /locus_tag="VFO47_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8788295.1"
                     /translation="MSKAGLTAADLSAARRREPAAVTRVYTAYAPALFRFFMAAVSDR
                     HLAEDLTGTAFVSAIEALPRFRGPVEALGGWLFQIARHDLYDHRRKQARSRIEPLDDN
                     LNEAALADGAVDPEEVAIERLEGSRVMAALAELSPDQREVLLLRMAGGLTAPEVAETL
                     GKTTGAVKALQHRGLASLARVLGLRSPHEAQERPYPSPDSGRLTSQEEEER"
     gene            1163..1402
                     /locus_tag="VFO47_06075"
     CDS             1163..1402
                     /locus_tag="VFO47_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET8788296.1"
                     /translation="MGPIPVEIQPWYERAACLDKDADCFFPEKGGSTRAAKRICQTCT
                     VQTECLEYALANDERFGIWGGLSERERRRLKRRAS"
     gene            1568..>2245
                     /locus_tag="VFO47_06080"
     CDS             1568..>2245
                     /locus_tag="VFO47_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8788297.1"
                     /translation="MPASQPRVIAIVVNHNGRAFLRDTFRGLAAQTRPIDDVLVVDTG
                     STDGSADWARSRLGDDAVMAVRGQFGRAVMSALRDPRTAGMDWLWLLHDDCAPEPEAL
                     ERLLAEAESRPSASILGPKLVSWTNPDRLSEIGFSIDRTGRAVSPIEDDEIDQGQHDQ
                     IQDVFFVNTAGMLVRRGALLNVGGFDERMPAFRDDLDLCWRTHLTGGRVLVVPQARVR
                     HFAAAASR"
BASE COUNT          311 a          809 c          785 g          340 t
ORIGIN      
        1 tgccaccgcg accacgcggc ggcgcatgtc ccagccgctg ggctggcggc gcacgggcag
       61 ctccaggacc gtgcccgagc gctccagggc ccgctcctgg tggcggtcgg ccacctcggg
      121 gtcgagctgg aaggtggcca gctcggcgcg cagagcgtcg gccgcctcca ccagcggcgc
      181 aagctcgtcg gtgagctcga caggacgccc gtcgagcagg gcgtcgagct catcgtggag
      241 cttgttcggc tttggctgcg gcgttttcac cgttcctcct cctcctgact agtcaggcga
      301 ccggagtctg gtgatggata cgggcgttcc tgtgcctcgt gcgggctacg taggcccaat
      361 actctggcaa ggctagccag gccacggtgc tgcagggcct tgacggcgcc ggtggtcttg
      421 cccagcgtct cggccacctc gggggcggtc agcccgccgg ccatccgcag cagcaggacc
      481 tcgcgctggt cgggggagag ctcggccagc gcggccatca ccctgctccc ctccaggcgc
      541 tcgatggcca cctcctcggg gtcgacggcc ccgtcggcga gggcggcctc gttgaggttg
      601 tcgtcgagcg gttcgatccg ggagcgggcc tgcttgcgcc ggtggtcgta gaggtcgtgg
      661 cgggcgatct ggaacagcca gccgccgagg gcctcgaccg ggccgcggaa gcgggggagg
      721 gcctcgatgg cgctgacgaa ggcggtgccg gtgaggtcct cggccaggtg gcggtcgctg
      781 accgcggcca tgaagaagcg gaacagggcg ggggcgtagg cggtgtagac gcgggtcacg
      841 gcggccggct cgcggcggcg cgcggccgac aggtccgccg cggtcaggcc ggccttggac
      901 accgcctccc gctctccccc gaagccgagc actcctaccc ccgatgcacc tcgtccgccg
      961 gtgaggctat cccatgcacc ggtcacgggc cacacatccg gatcctgcgc gactccccga
     1021 cctgtccaca gaaaaacctt ccgcttgaaa atgccactca agtcgccgca gaatgccacc
     1081 gatgtaatta cgatcgtgat cttccaaaca cccgtaagcg tgcgacccgg acgcctcgct
     1141 gcaccggttg aggaggcaat ggatgggccc catccccgtc gagatccagc cctggtacga
     1201 gcgggcggcc tgcctggata aggacgccga ctgcttcttt ccggagaagg gcggctccac
     1261 ccgcgcggcc aagcgcatct gccagacctg caccgtgcag accgagtgcc tcgagtacgc
     1321 gctggccaac gacgagcggt tcgggatctg gggcggcctg tccgagcgcg agcgccgccg
     1381 gctcaagcgc cgcgcctcct gacacaaatt aggcggtgcc cggttcgcgt cttttgacgg
     1441 ttgctttagg gctggctggc cgtcccgtgc tagcattccc ggacgcccgt gggggagctt
     1501 ggaaccctac gggcctttcg atacaccccc ggctaccccc ggccggcgag gcctcgagag
     1561 gaaggttgtg ccagcgagtc agccccgggt catcgcgatc gtcgtcaacc acaacgggcg
     1621 ggcgttcctt cgcgacacct tccgcggtct cgccgcccag acccgcccga tcgacgacgt
     1681 gctggtggtc gacaccggct cgaccgacgg cagcgccgac tgggcccgct cgcgcctggg
     1741 tgacgacgcc gtcatggcgg tcagggggca gttcggccgg gcggtgatgt cggccctgcg
     1801 cgacccccgg acggcgggca tggactggct gtggctgctg cacgacgact gcgcgcccga
     1861 gccggaggcg ctggagcgcc tcctggccga ggccgagtcg cggcccagcg cctccatcct
     1921 cggtcccaag ctcgtctcct ggaccaaccc cgaccgcctc tccgagatcg gcttctcgat
     1981 cgaccgcacg ggccgggcgg tgtccccgat cgaggacgac gagatcgacc agggccagca
     2041 cgaccagatc caggacgtgt tcttcgtcaa caccgccggc atgctcgtgc ggcgcggcgc
     2101 cctgctgaac gtcggcggct tcgacgagcg catgcccgcc ttccgtgacg acctcgacct
     2161 gtgctggcgc acccacctga ccggcggtcg ggtgctggtg gtgccccagg cccgggtccg
     2221 ccacttcgcc gccgccgcct cgcgc
//
LOCUS       DASSWR010000367         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1229658, whole genome shotgun sequence.
ACCESSION   DASSWR010000367 DASSWR010000000
VERSION     DASSWR010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229658"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFO47_06085"
     CDS             complement(<1..641)
                     /locus_tag="VFO47_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8788298.1"
                     /translation="MADAVADVKRAARERVEQARDELVALSHRIHANPELGFEEEQAC
                     AWLCELLDGAGLEVERGVGDLPTAFAARAGSGPLHVVVCAEYDALPAVGHACGHNVIA
                     AMAAGAGLALARVADDAGLRVTVLGTPAEEGGGGKILLLQRGAFDGAHAAMMVHPSPY
                     EQPEMPIIAVSHLEIAYTGKEAHASAYPFEGVNAADALVVAQTAIGLLRQHLRP"
     gene            complement(634..1398)
                     /locus_tag="VFO47_06090"
     CDS             complement(634..1398)
                     /locus_tag="VFO47_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788299.1"
                     /translation="MDDQTGGGLPASIAAAWGLRERPHKGPRPALSLERIVAAAVRVA
                     AADGLQAVSMGRVASELGVSTMSLYRYVGAKDELLALMADLTFEGPPARRGPEESWRD
                     GLARWARTELGVYRRNPWVLRIPISGPPVTPNAMAWLERGLDCLADTGLQEGEKLSAM
                     LLLTGFVRGQAALQADIAAAQAAGAAAPDPEMMGAYGRLLARLLDPERFPALHAVIAA
                     GVFDEPDDGDYDFNFGLERVLDGIETLIDKRRSGRG"
     gene            1490..2479
                     /locus_tag="VFO47_06095"
     CDS             1490..2479
                     /locus_tag="VFO47_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788300.1"
                     /translation="MHDELAVEAIGLEKSYGRVRVLDGVDLGVARGGVFALLGPNGAG
                     KTTAVRILATLVRADAGQVRVAGFDVAADRRQVRRRISLTGQFAAVDDLQTGEENLRM
                     MGRLAGLSRARARRRAAELLERFDLTDAAGRTVKTYSGGMRRRLDLAAGLVGDPAVLF
                     LDEPTTGLDPRSRQAVWQVVGELAGSGVSVFLTTQYLEEADRLADRIAVLDGGRVVAE
                     GTSAQLKEQVAGHRLDLVLADAAAFEEVARRLDDRAARRDPARLTIGVATDGSAAQVR
                     ALLDEVDPARRMVDRFAVHGATLDDVFLALTGHPAGQPDHDYENEHRKEPANV"
BASE COUNT          314 a          984 c          925 g          325 t
ORIGIN      
        1 gggcgcaggt gctggcggag caggccgatc gccgtctggg ccacgaccag cgcgtcggcg
       61 gcgttgacgc cctcgaacgg gtaggccgag gcgtgggcct ccttgccggt gtaggcgatc
      121 tcgaggtggc tgacggcgat gatcggcatc tcgggctgct cgtacggcga ggggtggacc
      181 atcatggccg cgtgcgcccc gtcgaaggcg ccccgctgga gcagcaggat cttgccgccg
      241 ccgccctcct cggccggggt cccgagcacg gtcacgcgca ggccggcgtc gtcggccacc
      301 cgggccaggg ccaggccggc cccggcggcc atggccgcga tcacgttgtg cccgcaggcg
      361 tggccgacgg ccgggagggc gtcgtactcg gcgcacacga ccacgtgcag cggccccgac
      421 ccggcccggg ccgcgaacgc cgtcggcagg tcgccaaccc cccgctcgac ctcgaggccg
      481 gccccgtcca gcagctcgca cagccaggcg caggcctgct cctcctcgaa cccgagctcg
      541 gggttggcgt ggatgcggtg gctgagggcc accagctcgt ccctggcctg ctcgacccgc
      601 tcccgggccg cccgtttcac gtcggcgacg gcgtcagcca cgtccgctcc tcctcttgtc
      661 gatcagggtc tcgatcccgt ccagcacccg ctccagaccg aagttgaagt cgtagtcgcc
      721 gtcgtccggc tcgtcgaaca ccccggccgc gatcaccgcg tgcagggccg ggaaccgctc
      781 cgggtcgagc agcctggcca gcaggcgccc gtaggcgccc atcatctccg ggtcgggcgc
      841 ggccgccccg gccgcctggg cggcggcgat gtcggcctgg agcgccgcct gccccctgac
      901 gaagccggtg agcagcagca tcgccgagag cttctcaccc tcctggaggc cggtgtccgc
      961 caggcagtcc agcccacgct ccagccaggc catggcgttg ggggtgaccg gcgggccgct
     1021 gatcgggatg cgcagcaccc acgggttgcg gcggtacacc ccgagctccg tcctggccca
     1081 gcgggccagc ccgtcgcgcc agctctcctc cggcccccgc cgggccggcg gcccctcgaa
     1141 ggtcaggtcg gccatcaggg ccagcagctc gtccttggcc ccgacgtagc ggtacagcga
     1201 catggtggag acgccgagct cgctggccac ccggcccatc gacaccgcct ggaggccgtc
     1261 ggcggccgcg acccgcacgg ccgcggccac gatccgctcc aggctgaggg cgggtcgcgg
     1321 gcccttgtgg ggccgctcgc gcagccccca ggcggccgcg atgctggccg gcaggccgcc
     1381 gccggtctgg tcgtccatgg tctccgcttc cggtgggggt tgacgcccat cctacgattg
     1441 tgtatacctt acacagtaaa gtgtatgcca tacacagaag gagcgtgaga tgcacgacga
     1501 gctggccgtc gaggcgatcg gcctcgagaa gtcctacggc cgggtgcggg tcctggacgg
     1561 cgtcgacctg ggcgtggccc ggggcggcgt gttcgccctg ctcggcccca acggggccgg
     1621 caagaccacg gccgtgcgga tcctggccac cctggtccgg gccgacgccg gccaggtccg
     1681 ggtggccggc ttcgacgtcg ccgccgaccg ccgccaggtg cgccgccgca tcagcctcac
     1741 cggccagttc gcggccgttg acgacctcca gacgggcgag gagaacctgc gcatgatggg
     1801 ccgcctggcc ggcctgtccc gcgcccgggc ccggcgccgg gcggccgagc tgctggagcg
     1861 gttcgacctc accgacgccg ccggccgcac cgtcaagacc tactcgggcg gcatgcgccg
     1921 ccgcctcgac ctggccgccg gcctggtcgg cgacccggcg gtgctgttcc tggacgagcc
     1981 gaccaccggc ctggacccgc gcagccgcca ggcggtgtgg caggtcgtgg gcgagctcgc
     2041 cgggtcgggg gtgagcgtgt tcctgaccac ccagtacctg gaggaggccg accggctggc
     2101 cgaccgcatc gccgtcctcg acggcggccg ggtggtcgcc gaggggacct cagcccagct
     2161 caaggagcag gtcgccggcc accgcctcga cctggtgctg gccgacgcgg ccgccttcga
     2221 ggaggtcgcc cggcgcctgg acgaccgggc cgcccggcgc gacccggccc gcctgaccat
     2281 cggggtcgcc accgacggca gcgccgccca ggtccgggcc ctgctcgacg aggtcgaccc
     2341 ggcccgccgg atggtcgacc ggttcgccgt ccacggcgcc accctcgacg acgtgttcct
     2401 ggccctgacc ggccacccgg ccgggcagcc cgaccacgac tacgagaacg agcaccggaa
     2461 ggagcccgcg aatgtctagc tacggccgac tcctcgatcg cggctggggg accgtgccac
     2521 ctgccggctg ggccgttgtc gccgactg
//
LOCUS       DASSWR010000368         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1003533, whole genome shotgun sequence.
ACCESSION   DASSWR010000368 DASSWR010000000
VERSION     DASSWR010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003533"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFO47_06100"
     CDS             <1..657
                     /locus_tag="VFO47_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8788301.1"
                     /translation="TVGLQYTTASNAGFITGLSVVLTPLLGALLLRQAPGRWPVTGAL
                     VAAAGLALLSLQRLEVHHGDALVLGCAVAFAAHILLLGRYAPRFPAYRLAVLQLGTAG
                     LLGLAWAGVAGDLAVPPSAEVWVALAITSVVASAAAFLIQTRAQREVSPTRTAVIFTM
                     EPVFAGLFGFLLAGERLSGRGWLGACLILAGMLIAELGGRQPAAPGHPQTDGGQPCPS
                     "
     gene            complement(1012..1194)
                     /locus_tag="VFO47_06105"
     CDS             complement(1012..1194)
                     /locus_tag="VFO47_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788302.1"
                     /translation="MASSSRRTSTARKKQARQLLELALPLATTAGPTPGRSRTSAANG
                     GEVHSGSWAGCTSYIP"
     gene            1416..2150
                     /locus_tag="VFO47_06110"
     CDS             1416..2150
                     /locus_tag="VFO47_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HET8788303.1"
                     /translation="MDLHSSEQAWIASPVWALVQALATLTGPDEEIRVAGLDGGPGPG
                     DGDRRLLAELAAGFDPAEHLREANAARYRLPGGPAELLEALLFRPTMNISGIAAGYVG
                     PGGKTIVPSRAEARVDIRLVGGDPEAAAAAVRRHLDEHGFGHVQVRLLDAYPWAKAPP
                     GNRSELAMRASYAAMGRSTLPYPLAPWCAPYFVFDRVLGLPWASGGVGHAAGAHGPDE
                     YATLAGLEEHVVGAAAFLLAYADLPS"
     gene            2147..>2571
                     /locus_tag="VFO47_06115"
     CDS             2147..>2571
                     /locus_tag="VFO47_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788304.1"
                     /translation="MSPVRVVVARGDPLERGRTVGYELADLIRGSLEFYWRYLVRRGV
                     RAGELAGLLAPYLAAAERELPDLVELVTGMAEGSGVSFWELFCVNAFEELEPLLEDRG
                     GRSLGQVTGAGGPEEHCSTVTISGPGYTLLGHNEQWLAG"
BASE COUNT          263 a          978 c          973 g          357 t
ORIGIN      
        1 acggtcgggc tccagtacac caccgcctcc aacgccggct tcatcaccgg cctctcggtg
       61 gtgctcaccc ccctgctcgg ggcgctgctg ctgcgccagg cgccgggccg ctggccggtg
      121 acaggggcgc tggtggccgc cgccgggctg gccctcctgt cgctgcagcg cctggaggtc
      181 caccacggcg acgcgctggt gctcggctgc gccgtcgcct tcgccgccca catcctgctg
      241 ctcggccgct acgctccccg cttcccggcc taccggctgg ccgtcctcca gctgggcacg
      301 gccgggctgc tgggcctggc ctgggcgggc gtggccggcg acctggccgt ccccccttcg
      361 gccgaggtgt gggtcgccct ggccatcacc tcggtggtcg cctcggccgc cgccttcctg
      421 atccagaccc gggcccagcg ggaggtgtcc ccgacccgca ccgccgtcat cttcaccatg
      481 gagccggtgt tcgccggcct gttcgggttc ctgcttgccg gcgagcgcct gtccgggcga
      541 ggctggctcg gcgcctgcct gatcctggcc ggcatgctga tcgccgagct gggcggccgg
      601 cagccggccg ccccgggcca tccccagacc gacggaggcc agccatgccc gagctgagct
      661 ggcgctacgg ctacgcctgg gcggtcgggc tgatggtggc gtgcgcgggc gtcctgtggg
      721 tcgacttcaa gcggaacggg ggctggctgt agagaatacg gggatggacg acacgctggc
      781 ccgggtgcga gcccgggtgg tcgagcgctt cccggccgac ctggagcgga tccgcggcta
      841 cctgcggatg ccgtcggtga gcgccaccgg cgaggggatc cgggaggcgg ccgaggccac
      901 cggggcctgg atcgagggcg ccggcggctc cttcgagctc gtcgggaccc ccggccaccc
      961 gctggtcctt ggcgagctgc ccggcccggc gggcgcgccc cggctgctgc gctacgggat
     1021 gtacgacgtg cagccggccc aggagccgga gtggacctcg ccgccgttcg cggccgaggt
     1081 ccgcgacctc cccggcgtcg gcccggcggt ggtggccagg ggcagcgcca actccaagag
     1141 ctgcctggcc tgcttcttcc tggccgtcga ggtcctgcgc gagctggacg ccatgccgct
     1201 gaccgtcgtc ctgatgatcg agggcgagga ggagctgggc agccccaacc tggccgccgc
     1261 cgtccaggcc aggtcgggcg acctgaccgc cgagggcgcc ttcgacctcg acctgaccgc
     1321 cggcctggac ggccagccgg agctgatcct cggctgcaag ggcctgtgcg acctggagct
     1381 ggtcgccgag gccggcgact ggggcgggcc cggccatcga cctccactcc agcgagcagg
     1441 cctggatcgc ctcgccggtg tgggccctgg tccaggccct ggccaccctg accggccccg
     1501 acgaggagat ccgggtggcc ggcctcgacg gcggccccgg gcccggcgac ggcgatcggc
     1561 ggctgctggc cgagctggcc gccggcttcg acccggccga gcacctgcgc gaggccaacg
     1621 ccgcccgcta ccggcttccc ggcgggccgg ccgagctgct ggaggcgctg ctgttccggc
     1681 cgaccatgaa catctccggg atcgccgccg gctacgtcgg ccccggcggc aagacgatcg
     1741 tgcccagccg ggccgaggcc cgggtcgaca tccgcctggt cgggggcgac ccggaggcgg
     1801 ccgcggccgc cgtccgccgc cacctcgacg agcacgggtt cggccacgtc caggtgcgcc
     1861 tgctcgacgc ctacccgtgg gccaaggcgc cgcccggcaa ccgctccgag ctggccatgc
     1921 gggcctcgta cgcggccatg ggccggtcaa cgctgccgta cccgctggcg ccgtggtgtg
     1981 cgccctactt cgtgttcgac cgggtcctcg gcctgccctg ggcgtcgggc ggggtcgggc
     2041 acgccgccgg cgcccacggg cccgacgagt acgccaccct ggccgggctg gaggagcacg
     2101 tcgtcggcgc cgccgccttc ctgctggcct acgcggacct gccctcatga gcccggtgcg
     2161 ggtggtggtg gcccgcggcg acccgctgga gcggggccgc accgtcggct acgagctggc
     2221 cgacctgatc cgcggctcgc tggagttcta ctggcgttac ctggtccgcc gcggggtccg
     2281 ggccggcgag ctggccgggc tgctcgcgcc ctacctggcc gcggccgagc gggagctgcc
     2341 cgacctggtc gagctggtca ccgggatggc cgaggggtcc ggggtgtcgt tctgggagct
     2401 gttctgcgtc aacgccttcg aggagctgga gccgctgctg gaggaccgcg gcggccgctc
     2461 cctgggtcag gtcaccggcg ccggcggccc cgaggagcac tgctcgacgg tcaccatctc
     2521 cgggcccggg tacaccctgc tcggccacaa cgagcagtgg ctggccgggg a
//
LOCUS       DASSWR010000369         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_861947,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000369 DASSWR010000000
VERSION     DASSWR010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861947"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFO47_06120"
     CDS             complement(<1..886)
                     /locus_tag="VFO47_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788305.1"
                     /translation="MSTALSPAGVEVRDAGGSDLPAVRRVLLAAYQEYAMALPPAVFG
                     RYLAEILDVEERLENGRLLVAEHGGRVVGTVTWYDDAATEGLGWPAGWAGLRALGVEP
                     SARGLGIGRALVEACRRRALAAGAPALTLHTGGFMTAAVALYEAMGFRRDPAYDFDAT
                     RRLRLGGVRSVPILAYRLDLTRHHAKERIMGGPAAIVGELYENPVTGERGVVRVTPTG
                     ANGHLLVVDLYLSPGGRVAGEHVHPVTTEAFTVVRGQLAVRHHGRDRRAGPRTRFQIA
                     PAWPTTSGTPPARRSASWW"
     gene            complement(942..>2358)
                     /locus_tag="VFO47_06125"
     CDS             complement(942..>2358)
                     /locus_tag="VFO47_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET8788306.1"
                     /translation="PLQPVPVRGGSGPGRPGGRPAGADRRPGELGQALTTLSRMRYMV
                     NDPAGSEQAVTRAVDLLAPLGDLPRLARASTYLAAIMKLTDRLDEAIAQARQALELGE
                     RTGQGDVVAHSLNYLGYALLDLGDPGGAAHLRRSAEVARAAAHYEYAQRAYTNLVEGL
                     YRLGRFGELDQPLAEGLAYAREYGFASHEYNLEAHRCMLLTLRGRWDEAEAGLRRLLA
                     GEDPGVLASFGLSALGRLLARKGDPAAGPLLERAWRTAARTNSVQAIALAGIARVESA
                     WLAGDHRAAAEVARVPLERTSGRGAERYRGELLRYLARCRHPVEAPAGCPPEFALGIE
                     GDWAAAAELWRSLGAPYEHALELAASGRQAELLEAAGALDRLGAVAAANLVRRELRRL
                     GVTHLPRRPRPRTRANPAGLTDRQLEVLQLLAEGLTNAEIADRLVVSVRTVDHHVAAI
                     LSRLNVGSRREAARAAADLGA"
BASE COUNT          276 a          874 c          949 g          259 t
ORIGIN      
        1 tccaccagga cgcggacctc ctcgccggag gcgttccaga agtcgtgggc cacgccgggg
       61 cgatctggaa ccgggtccgg gggccggccc gccggtcgcg gccgtggtgg cgcacggcga
      121 gctggccccg gacgaccgtg aaggcctcgg tggtgaccgg gtggacgtgt tccccggcga
      181 cccttccgcc cgggctcagg tacaggtcga ccaccagcag gtggccgttg gccccggtgg
      241 gcgtgacccg cacgaccccg cgctctccgg tgaccgggtt ctcgtacagc tcgccgacga
      301 tggctgccgg gccgcccatg atgcgctcct tggcatggtg ccgggtgagg tcgaggcggt
      361 aggcgaggat cgggacggac cggaccccgc cgagccggag ccggcgggtg gcgtcgaagt
      421 cgtaggccgg gtcgcgccgg aagcccatcg cctcgtacag ggccaccgcg gccgtcatga
      481 acccgccggt gtggagggtg agggcgggcg cgccggcggc cagggcccgc cgccggcagg
      541 cctcgaccag ggcccggccg atgcccagcc cgcgggccga gggctccacc ccgagggcgc
      601 gcagcccggc ccacccggcc ggccacccga gcccctcggt ggcggcgtcg tcgtaccagg
      661 tgaccgtgcc cacgacccgc ccgccgtgct cggcgaccag cagccggccg ttctccaagc
      721 gctcctcgac gtcgaggatc tcggccaggt agcggccgaa gacggccggc ggcagggcca
      781 tggcgtactc ctggtaggcg gccaggagca cccgccggac ggccggcagg tcggacccac
      841 cggcgtcgcg gacctcgacg ccggccgggc tcagggcggt ggacatcgtg ccccgaccgt
      901 agcggcgccc cggccggggc acatcggcaa tcgctgccta tctacgcccc gaggtcggcg
      961 gcggcgcggg cggcctcccg gcgcgacccg acgttcagcc tggacaggat ggcggcgacg
     1021 tgatggtcga cggtgcggac cgagaccacc aggcggtcgg cgatctcggc gttggtcagg
     1081 ccctcggcga gcagctgcag gacctcgagc tggcggtcgg tgaggccggc cgggttggcc
     1141 cgggtccggg gccgggggcg gcgggggagg tgggtgacgc cgaggcgccg cagctcccgg
     1201 cggaccaggt tggcggcggc cacggcgccg agtcggtcga gggcccctgc cgcctccagc
     1261 agctccgcct ggcggcccga ggcggccagc tccagggcgt gctcgtaggg ggcgccgagc
     1321 gaccgccaca gctcggccgc ggccgcccag tcgccctcga tgccgagggc gaactccggc
     1381 gggcagcccg ccggcgcctc caccgggtgc cggcagcggg ccaggtagcg gagcagctcg
     1441 ccccggtagc gctcggcccc gcggcccgag gtgcgctcca gcgggacccg ggccacctcg
     1501 gccgcggccc ggtggtcgcc ggccagccag gccgactcca cccgggcgat gccggccagg
     1561 gcgatcgcct ggaccgagtt ggtccgggcg gccgtgcgcc aggcccgctc cagcagcggc
     1621 ccggcggccg ggtcgccctt gcgggccagc agccggccca gcgccgacag cccgaagctg
     1681 gccaggacgc ccgggtcctc gccggccagc agccggcgca gcccggcctc ggcctcgtcc
     1741 cagcggcccc gcagggtgag cagcatgcag cggtgggcct ccaggttgta ctcgtgggag
     1801 gcgaagccgt actcgcgggc gtaggccagg ccctcggcca gcggctggtc gagctcgccg
     1861 aagcggccca ggcggtacag cccctcgacc aggttggtgt aggcccgctg ggcgtactcg
     1921 tagtgggcgg cggcccgggc gacctcggcc gagcgccgca ggtgggccgc gccgcccggg
     1981 tcgcccaggt cgagcagggc gtagccgagg tagttgagcg agtgggccac gacgtcgccc
     2041 tggccggtgc gctcgcccag ctccagggcc tggcgggcct gggcgatggc ctcgtcgagg
     2101 cggtcggtga gcttcatgat ggcggccagg taggtggagg cgcgggccag ccggggcagg
     2161 tcgccgagcg gcgccagcag gtccacggcg cgggtcacgg cctgctcgga cccggccggg
     2221 tcgttgacca tgtagcgcat ccgcgacagg gtggtgaggg cctgcccgag ctcgccgggt
     2281 cgccggtcgg ctcccgcagg gcgaccaccc ggccggcccg gtccgctgcc tcctcgaacc
     2341 gggacaggtt gtagagga
//
LOCUS       DASSWR010000370         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1173200, whole genome shotgun sequence.
ACCESSION   DASSWR010000370 DASSWR010000000
VERSION     DASSWR010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173200"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1078)
                     /locus_tag="VFO47_06130"
     CDS             complement(65..1078)
                     /locus_tag="VFO47_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8788307.1"
                     /translation="MTDIAARHDALQGDQALPEVLERFRPELTGYCYRMLGSAFEAED
                     AVQETMVRAWRGHDRFEGRSALRSWLYRIATNVCYDHLNGRQRRARPMDLGPAQPATT
                     PRVPPPEPEAVWINPIPDARVVPDGADPAEVAEARESIRLAFIAALQLLPPRQRAVLI
                     LRQVLRWKASEVAELLDTSVASVNSLMQRAKATLEAADTTAGELARPLDEDQQELLAR
                     YVDAFERYDMDALTALLHDDATMSMPPIPLWLQGADQIVAWNLGHGKGCRGSRFVPAP
                     AANGSPAYGQYRPSGPGGRHEAWSLHVLDIRDERIARIEVFLDVERVFPLFGLPLRLD
                     DEG"
     gene            1219..2019
                     /locus_tag="VFO47_06135"
     CDS             1219..2019
                     /locus_tag="VFO47_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HET8788308.1"
                     /translation="MEDHVVTDGAGFRLADRDPGATSGVPGKAEGRAEVAELTRRMRH
                     LQERFYADGRHRLLVVLQGMDTSGKSGAIRRAFAGLNPAGVRVTSFRTPTDDELARDF
                     LWRVHRHVPRNGEIALFDRSHYEDVVAVRVRGLVPPERWRARYEHIRAFERMLVDEGT
                     IVRKFFLHISREEQATRLRARLEDPRKHWKFRRSDLEDRDRWDDYQAAYEDAIRETAT
                     PEAPWVIVPADRKWHRDLVICRTIVDTLERLDLRYPEAEEDLSGVVVV"
     gene            complement(2026..>2464)
                     /locus_tag="VFO47_06140"
     CDS             complement(2026..>2464)
                     /locus_tag="VFO47_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8788309.1"
                     /translation="DGDWGLDGSVGIAPRPVQASVPIWVGGSSPAAVRRAADLADGWL
                     PQGVRLRDLPGIVARVRERREAAGRPAAFDVGALFVCHVGDPTWDVGRCLAGPPERVA
                     EGLRPWIEAGANQLQVRLRSRDAAELCDQVERFGAEVAPLLTP"
BASE COUNT          310 a          912 c          945 g          297 t
ORIGIN      
        1 tattctcgtc ggtgccgaac gcgatgcgca tcgccgccca ggctagggcg gcgccgccgg
       61 gcggctaccc ctcgtcgtcg aggcgcagcg gcaggccgaa gagcgggaag acccgctcga
      121 cgtcgaggaa cacctcgatg cgggcgatcc gttcgtcgcg gatgtcgagg acgtgcagcg
      181 accaggcctc gtgccggccg ccggggccgc tcggcctgta ctggccgtag gccggcgagc
      241 cgttggccgc cggcgcgggc acgaaccggg agccgcggca ccccttcccg tggcccaggt
      301 tccaggcgac gatctggtcg gcgccctgga gccacagcgg gatgggcggc atggacatgg
      361 tggcgtcgtc gtgcagcagc gccgtgagcg cgtccatgtc gtagcgctcg aaggcgtcga
      421 cgtagcgggc gagcagctcc tgctggtcct cgtcgagcgg ccgggccagc tcgcccgcgg
      481 tcgtgtcggc cgcctcgagg gtggccttgg cccgctgcat caggctgttg accgacgcca
      541 ccgaggtgtc gagcagctcg gccacctcgc tggccttcca ccgcagcacc tggcggagga
      601 tgagcaccgc ccgctggcgg ggcggcagca gctgcagggc ggcgatgaag gccaggcgga
      661 tcgactcccg ggcctcggcc acctcggccg ggtcggcgcc gtcgggcacg acccgcgcgt
      721 cggggatggg gttgatccag acggcctcgg gctcgggcgg cgggaccctc ggcgtggtcg
      781 ccggctgggc cgggcccagg tccatcggcc gggcccgccg ctggcgcccg ttcaggtggt
      841 cgtagcagac gttggtggcg atgcggtaga gccacgaccg cagcgccgag cggccctcga
      901 accggtcgtg gccgcgccac gcccggacca tcgtctcctg cacggcgtcc tcggcctcga
      961 aggccgagcc gagcatccgg tagcagtagc cggtgagctc ggggcggaac cgttcgagca
     1021 cctcggggag ggcttggtcc ccctgcagag cgtcgtgccg tgccgcgatg tccgtcatgg
     1081 gccgcaccct ccctggcgtc ggcgtgcggg cgggcggcgc ccgcgccgtg tccgatgcta
     1141 cggcgatccg tcgccccgac cacgccgttt gcgcccgggc cggtccgacg gcccgccacg
     1201 ggggtagacc tggcggccat ggaggaccac gtggtgaccg acggcgccgg cttccgcctg
     1261 gccgaccgcg accccggcgc gaccagcggg gtgccgggca aggccgaggg ccgggccgag
     1321 gtggcggagc tcacccggcg gatgcggcac ctgcaggagc gcttctacgc cgacggtcgc
     1381 caccgtctgc tggtcgtgct gcaggggatg gacacctccg gcaagagcgg cgccatccgc
     1441 cgggccttcg ccggcctgaa ccccgccggg gtccgggtga cgtcgttccg cacgccgacg
     1501 gacgacgagc tggcccgcga cttcctctgg cgggtgcacc ggcacgtgcc ccgcaacggg
     1561 gagatcgccc tgttcgaccg gagccactac gaggacgtcg tcgccgtccg ggtgcggggc
     1621 ctggtgccgc ccgagcggtg gcgggcccgc tacgagcaca tccgggcctt cgagcggatg
     1681 ctggtcgacg agggcacgat cgtccgcaag ttcttcctcc acatctcccg ggaggagcag
     1741 gcgacgcgcc tgcgggcccg gctggaggac ccgcgcaagc actggaagtt ccgccgcagc
     1801 gacctcgagg accgggaccg gtgggacgac taccaggcgg cctacgagga cgccatccgc
     1861 gagaccgcca cgccggaggc gccgtgggtg atcgtccccg ccgatcgcaa gtggcaccgg
     1921 gacctggtca tctgccgcac gatcgtcgac acgctcgagc ggctcgacct ccgctacccg
     1981 gaggcggagg aggacctctc cggcgtggtg gtcgtgtgag ccggctcagg gggtgagcag
     2041 cggggcgacc tcggccccga agcgctcgac ctggtcgcac agctcggcgg cgtcccgcga
     2101 ccgcagccgg acctgcagct ggttggcgcc cgcctcgatc cacgggcgga ggccctcggc
     2161 gacccgctcg ggcgggccgg ccaggcaccg gccgacgtcc caggtcgggt cgccgacgtg
     2221 gcagacgaac agcgcgccga cgtcgaaggc cgccggccgg cccgcggcct cccgccgctc
     2281 ccggacccgg gcgacgatgc cgggcaggtc gcgcaggcgc acgccctggg gcagccagcc
     2341 gtcggccagg tcggccgccc gccggaccgc cgccggcgac gagcccccga cccagatcgg
     2401 cacggacgcc tggaccgggc ggggcgcgat gcccaccgag ccgtcgagcc cccagtcgcc
     2461 gtcg
//
LOCUS       DASSWR010000371         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_946732,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000371 DASSWR010000000
VERSION     DASSWR010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946732"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFO47_06145"
     CDS             <1..746
                     /locus_tag="VFO47_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788310.1"
                     /translation="PDPAGQPADGGLLLDAASGRSRPVKVGCPVAWAARAGVFAGAGG
                     PWTRVEGFLRAAATPKGGSVLIGRPGKRPRVLLDARRLRAEAGRGAVVDGLALSPDGK
                     LVAVSAGRPGGAWSVLVTGTDGRRVAAIPLAAGHRPAWIGWSDRQGSTTLAVAAVDRR
                     GGLATAELAARQAGGYVLAWRPGGGARLLASGVPMVAADGFAWSSDGDSVAISSPSGV
                     LVVKQADVVYTTATPVTGTLLAWPARAAP"
     gene            743..2062
                     /locus_tag="VFO47_06150"
     CDS             743..2062
                     /locus_tag="VFO47_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788311.1"
                     /translation="MSDPLIEQGRGRRRLAVVAPVVAVVCGLVVGGLAGERVAGRRDP
                     GAVPKTEARPDGVHGSSGPVEQLRGVGTLLFIDQQGRAHAQGADGRDRRVLGQLGELA
                     AQRAPSGADLEVAAHLLAFPPDDRPAELAPDADRVYLQTGDGAAAVGRLVDGSLRRLL
                     PAGWKAAGPPRHSADGSVLGVCGYRVGGGPIPGVTAATSWVLDGAGERLATLPGCLYD
                     VAADGGSALVADPAEGRRAGPVPGRELLRRPPVGPSDQLTRGLRLWRRDGGFRPVLGF
                     GEVVRVFRTVQPGADPRGLVIVTAVLGPDGRQAAVRIIDALADGHPRTSPEAEVLAVV
                     DLASGRAELIPEHLPGLFTFLPTGGRAWTGGAGTATYLPHDRPPQLLRSGIGNDAVYA
                     LVPSPDGAWLLLAGGATWRFIRADDPSVQVSYPAPGRLAAWVPAAGS"
     gene            2059..>2387
                     /locus_tag="VFO47_06155"
     CDS             2059..>2387
                     /locus_tag="VFO47_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788312.1"
                     /translation="MSSLRLALRGLRARAGLSAALLAVTTFAVGVAAAGAIYLRAAGE
                     SLLAQNLRTATSYTAGLHVSQVVADGRELRSLTGSLAAALPAVPALAPPVTGLETRTP
                     VPIRAEGG"
BASE COUNT          204 a          920 c          988 g          275 t
ORIGIN      
        1 gccccgaccc ggccgggcag ccggccgacg gcggcctgct cctcgacgcg gccagcgggc
       61 ggtcccggcc ggtcaaggtc ggctgcccgg tcgcctgggc ggcccgggcc ggggtgttcg
      121 ccggcgccgg cgggccctgg acccgggtcg agggcttcct gcgggccgcg gcgaccccca
      181 agggcggctc ggtgctgatc ggccggcccg ggaagcgccc ccgcgtgctg ctggacgcgc
      241 ggcggctgcg ggccgaggcg ggccgggggg cggtggtcga cggcctcgcc ctgagcccgg
      301 acggcaagct ggtcgcggtg tcggcgggcc ggccgggtgg ggcgtggtcg gtgctggtga
      361 ccgggaccga cgggcgccgg gtggccgcca tcccgctggc cgccgggcac cggccggcct
      421 ggatcggctg gtcggaccgg cagggctcga ccaccctggc cgtcgccgcc gtcgaccgcc
      481 gcggcggcct ggccaccgcc gagctggcgg ccaggcaggc gggcggctac gtgctcgcct
      541 ggcggcccgg cggcggcgcg cggctcctgg cctccggcgt gccgatggtc gcggccgacg
      601 gcttcgcctg gtcctcggac ggggactcgg tcgccatctc ctcgccctcg ggagtcctgg
      661 tcgtcaagca ggccgacgtc gtctacacca ccgccacccc cgtcaccgga accctcctgg
      721 cctggccggc ccgagcggcg ccatgagcga cccactcatc gagcagggcc ggggccggcg
      781 gcggctggcg gtggtggcgc cggtggtcgc ggtggtctgc gggctggtcg tgggcgggct
      841 ggccggggag cgggtggcgg ggcggcgcga cccgggggcc gtgccgaaga ccgaggcacg
      901 gccggacggg gtccacggga gcagcgggcc ggtggagcag ctgcgggggg tcgggacgct
      961 gctgttcatc gaccagcagg ggcgggccca tgcgcagggc gcggacgggc gggaccgccg
     1021 ggtgctgggg cagctggggg agctcgcggc ccagcgggcg ccgtcagggg ccgacctgga
     1081 ggtggccgcg cacctgctgg cgttcccccc cgacgaccgg ccggccgagc tggcacccga
     1141 cgccgaccgg gtctacctgc agacgggcga cggggcggcc gcggtggggc ggctggtcga
     1201 cgggtcgctg cggcggctgc tcccggccgg gtggaaggcg gccgggccgc cgcggcactc
     1261 ggccgacggg agcgtgctcg gggtctgcgg ctaccgcgtg ggcggggggc cgatccccgg
     1321 ggtgacggcc gccaccagct gggtcctgga cggggccggg gagcggctgg ccaccctgcc
     1381 cggctgtctc tacgacgtgg ccgccgacgg cggctcggcc ctggtcgccg acccggccga
     1441 gggtcgccgg gccggtcccg tgcccggccg ggagctgctc cggcggcccc cggtcggtcc
     1501 ctccgaccag ctcaccaggg gcctgcgcct gtggcggcgc gacggcggct tccgtcccgt
     1561 gctcggcttc ggcgaggtgg tccgggtgtt ccggaccgtc cagcccgggg ccgacccgcg
     1621 cggcctggtg atcgtgaccg ccgtgctcgg cccggacggg cgccaggccg cggtccggat
     1681 catcgacgcc ctggccgacg gccatccccg gacgagcccg gaggccgagg tcctggccgt
     1741 ggtcgacctg gccagcggcc gggccgagct catccccgag cacctgcccg gcctcttcac
     1801 gttcctcccc accggtggcc gggcctggac cggcggcgcc ggcacggcca cctacctgcc
     1861 ccacgaccgg ccgccgcagc tgctccgctc gggcatcggc aacgacgccg tctacgccct
     1921 cgtgcccagc cccgacggtg cctggctgct gctggccgga ggagccacct ggcggttcat
     1981 ccgtgccgac gacccctcgg tgcaggtgag ctacccggcc cccggccggc tggccgcctg
     2041 ggtcccggcg gccggctcat gagcagcctg cggctggctc tgcgcgggct gcgggcccgg
     2101 gccgggctgt cggcggcgct gctggccgtg accaccttcg ccgtcggggt ggccgcggcc
     2161 ggcgcgatct acctgcgggc ggccggggag tccctgctcg cccagaacct gcgcaccgcg
     2221 accagctaca ccgccggcct gcatgtcagc caggtcgtgg ccgacggccg ggagctgcgc
     2281 agcctcaccg ggtcgctggc cgcggccctg ccggccgtgc cggccctcgc cccgccggtc
     2341 accggcctgg agacccgcac cccggtcccg atccgggccg agggcgg
//
LOCUS       DASSWR010000372         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_325482,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000372 DASSWR010000000
VERSION     DASSWR010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325482"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFO47_06160"
     CDS             complement(<1..295)
                     /locus_tag="VFO47_06160"
                     /inference="COORDINATES: protein motif:HMM:NF020332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HET8788313.1"
                     /translation="MHQGCVAGANEGEEGSGGVVADIAKLSVGREAYYTHELATDHEA
                     YLSGHGESPGRWYGAGASPLGLEGEASVAGFPAMFEGRHPTTGELLGRPHGRNA"
     gene            complement(480..1715)
                     /locus_tag="VFO47_06165"
     CDS             complement(480..1715)
                     /locus_tag="VFO47_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788314.1"
                     /translation="MTRGARYRFLAVVYASQFVPLAFFLYGLSAVLREREVPLERIAF
                     VQLLGLVWVVKFAWAPLVDRYGSRRLGHYRSWLLAVQTLLTVAVLALIPVDVVTDLPL
                     LTGIGAAIALLSATHDIAADAAAVRLLAPSERGVGNGIQRAGGYLGLMVGGGGVLIIY
                     DRLGWGVALAVLAALTALPLPTLLGWREDQVLPGVAPRLGASFRTLGSYFRQPGATRW
                     ALVVLPLYYLGIATAYPLVTPMLVDAGWPLDRIGAVSIVGGGTAAVLASLASGALVTR
                     LGRRRTLAAFGLVTVVAIMALFPLAQGQSGTLAGLAAVALLNIAYASAGTAVYTINMD
                     WSRPGSAGSDFTVQDSLVHLCSLLTGAAGLALAGVLGYPRTLGLSVILGLAGVAAAAW
                     LFQQPSTPVVPVEASQRPV"
BASE COUNT          324 a          665 c          754 g          258 t
ORIGIN      
        1 cggcgttgcg gccatgcggg cggcctagca gctcccccgt tgtcgggtgg cggccctcga
       61 acatggccgg gaacccggcc accgatgctt cgccctccag ccccaggggg ctggcgccgg
      121 cgccgtacca ccggcctggg gactccccgt ggccagacag atacgcttcg tggtcggtgg
      181 ccagctcgtg ggtgtaatag gcctcccggc ccaccgagag cttggcgatg tcggccacca
      241 ccccgccgct cccctcctcg ccttcgttgg cgccagcaac gcacccctgg tgcattgctc
      301 tcctcagaaa atggtttcgg ggtgagagta gagcgaacgg cgagccttca tccgttatgg
      361 cgatacgtgg tggaggtggc tggttgtaag ccgtgagcgt ctattgggga ctgacgtcgg
      421 tcggcgcagg acggaagaag ccatgtatgc aactgacgac gttgaggtgg gatcgcggct
      481 cacaccgggc gctgagatgc ttcgaccggc accaccgggg tcgagggttg ctggaagagc
      541 caggccgcgg cagcgacgcc ggccaatccg aggatgaccg acaggccaag ggttcgcggg
      601 tagccgagga cgcccgccag ggccaggccg gcggccccgg tgagcagcga gcagagatgg
      661 accaggctgt cctgcacggt gaagtcgctg cccgccgacc cgggtcggct ccagtccatg
      721 ttgatcgtgt acaccgctgt gccggcgctc gcgtaggcga tgttgagcag cgcgactgcg
      781 gccagcccgg cgagcgtgcc gctctggccc tgcgccagcg ggaacagggc catgatggcg
      841 accactgtga ccagtccgaa ggcggccagc gtgcgccggc ggccgaggcg ggtcaccaac
      901 gctccgctcg cgagcgaggc cagcacggcc gcggttccgc caccgacgat cgagacggcg
      961 ccgatccggt ccagcggcca gccggcgtcg accagcatgg gggtgaccag ggggtaggcc
     1021 gtcgcgatgc cgaggtagta caggggcagg acgaccagcg cccatcgcgt ggccccgggc
     1081 tggcggaagt agctgcccag cgtccggaac gatgctccca ggcgcggggc gacgcctggg
     1141 agcacctggt cctctcgcca accaagcagc gtcggcagcg gcagcgcggt gagcgcggcc
     1201 aggacggcca gcgcgacccc ccacccgagc cggtcgtaga tgatcagcac gccgccgccg
     1261 ccgaccatca ggccgaggta gccgccggcc cgctggatgc cgttgccgac gccgcgctcg
     1321 gacggcgcga gcaggcggac agcggccgcg tcggcggcga tgtcatgggt cgccgagagc
     1381 aacgcgatgg cggcgccgat cccggtcagt agcggcaggt cagtgacgac gtcgaccggg
     1441 atgagggcga ggacggccac ggtcagtagc gtctggaccg ccagtagcca gctgcggtag
     1501 tggccgagcc gtcgtgagcc gtagcggtcg accaggggcg cccaggcgaa cttgaccacc
     1561 cagaccagcc ccagcagctg gacgaaggcg atccgctcca atggcacctc gcgctcgcgg
     1621 agcacggcgc tcagcccata gaggaagaag gccaggggaa cgaactggct ggcgtacacg
     1681 acggcgagga agcggtagcg ggcgcctctg gtcacgaggc ctccggtccg atggggcggc
     1741 tggtaacggg cgccgcggca acagctgatt ctcgcagcct ccctcgtgag cggccagatg
     1801 cggaacaatc aggcgtgagg acacgagagg ggatggggac ctggcccggg cgggcctgcg
     1861 cacccttgac tggtgatgct ctacggcagg gacgccgagc ggtcggcagt gacggcactg
     1921 ctggacgccg cccgggactc ccggagcggc gtgctggtgc tgcgcggcca ggctggtgcc
     1981 gggaagtcgg cgctgctcca g
//
LOCUS       DASSWR010000373         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_410143,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000373 DASSWR010000000
VERSION     DASSWR010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410143"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..150
                     /locus_tag="VFO47_06170"
     CDS             70..150
                     /locus_tag="VFO47_06170"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET8788315.1"
                     /translation="MADLRDRGAITEEEFQRMKRELLDRM"
     gene            213..1715
                     /locus_tag="VFO47_06175"
     CDS             213..1715
                     /locus_tag="VFO47_06175"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET8788316.1"
                     /translation="MAKQARAGGADRYHVKAREQGKLFVRERLARLLDEADGFVEDGL
                     LANALAGDLPADGVVTGVGAVGGRTVAVMANDSTVKAGSWGARTVEKILRVQEYALRH
                     EVPMLWLVDSAGARITDQVEMFPGRRGAGKIFYNQVRLSGRVPQICCLFGPSAAGGAY
                     IPAFCDVVFMVEGNASMYLGSPRMAEMVIGEKTTLEEMGGAAMHCRVSGCGDLLCADE
                     DEAIQAARRYLGYFPGSWRGDPPAEAPRGPAYPGPVAPLVPTDESQPFDVYPLVAAVV
                     DEESFFEVKREFAAEVVCGLGRIEGRAVGIVANQPLVKGGVLFVDSADKVARFVWLCD
                     AFNLPLVFLADVPGFMIGTEVERQGIIRHGAKLITAVSEATVPKICVVVRKAYGAGLY
                     AMAGPGFEPDATLALPTASIAVMGPEAAVNAVYYNKLAALPEAERPAEVERLQREYRE
                     DVDLVRLAADLVVDAVVDPEDLRSQLARRLAAAAGKDREFTRRRHGVPPV"
     gene            1712..3418
                     /locus_tag="VFO47_06180"
     CDS             1712..3418
                     /locus_tag="VFO47_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET8788317.1"
                     /translation="MNARPFRSVLVANRGEIAVRVLRACRELGLTGIAVHSDPDAGAL
                     HVREADRAVAIGGSAVAESYLDQDRLLAAAAEAGAEAVHPGYGLLSEHAGFARAVTGA
                     GLVWVGPPPEAIAQMGDKLAARRAVLAAGVEPVPGTTDPVTDPAEVVAFGEANGWPVA
                     VKAAAGGGGRGIRVVAGPGDAAEALAAATREAQAAFGDGACYLERFFPRPRHVEVQVL
                     ADTHGAIVQLGERDCSVQRRHQKLVEEAPCPGLPAEVGQRLRTWAVAVAKACGYVGAG
                     TVEFLWDPASGGAWFLEMNTRIQVEHPITEQVTGIDIVAAQLRVAAGEPLGLAQDDVA
                     VRGHAIECRVNAEDPARGFLPTPGTITALRWPGGPGVRVDAGYERGDTVPPFYDDLLG
                     KLVAWAPDRDQAIARMRAALDELVVEGVATTAPALARMLDHDDFRAARHWTTWLEAVD
                     LGGLAPAAAPEDGPDFHVTAGGRTWPVRLHRHRPRATGGGAGGGGLVASPLAGTLARL
                     TVTAGQRVAEGELLAVVEAMKMETPLLAPFSGIVVGVRSAVGGPVAAGQIVVELEAGG
                     RR"
     gene            3449..3865
                     /locus_tag="VFO47_06185"
     CDS             3449..3865
                     /locus_tag="VFO47_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019524140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788318.1"
                     /translation="MLEAQGFREFSGHADQVAAARRFVASAIQGGGPARDVSRLLVSE
                     AATNAVLHSATGDGGSFSVAYLISDHLLRVEVHDGGGPSGPRRRVHHLESMTGRGLEL
                     FDALSDRWGVDGSPDGWTVWFELDLAAEEARSDASR"
BASE COUNT          480 a         1470 c         1562 g          521 t
ORIGIN      
        1 ccgccggcgg ccgaccgcac cccgaccccg ccgatccgct cccggtccgc cctcgacgac
       61 ctggagcgcc tggccgacct gcgcgaccgc ggggccatca ccgaggagga gttccagcgc
      121 atgaagcgcg agctcctcga ccggatgtag ccgggtagga tcgtggcgtg accgacgaag
      181 cggcggagcg gccggagccg gcgttgcggg cactggccaa gcaggccagg gccggtgggg
      241 ccgaccgcta ccacgtcaag gcccgcgagc agggcaagct gttcgtgcgg gagcggctgg
      301 cccggctgct cgacgaggcc gacgggttcg tggaggacgg gctgctggcc aacgcgctgg
      361 ccggcgacct gccggccgac ggggtggtca ccggggtcgg ggcggttggc gggcgcacgg
      421 tggcggtgat ggccaacgac tcgaccgtca aggcggggtc gtggggtgcc cggacggtgg
      481 agaagatcct ccgcgtccag gagtacgcgc tgcgccacga ggtgccgatg ctgtggctgg
      541 tcgactcggc cggggcccgg atcaccgacc aggtggagat gttcccgggc cgccgcgggg
      601 ccgggaagat cttctacaac caggtgcggc tgtccggccg ggtgccgcag atctgctgcc
      661 tgttcggccc gtcggcggcc ggcggggcct acatccccgc cttctgcgac gtcgtcttca
      721 tggtcgaggg caacgcctcc atgtacctgg gctcgccccg catggccgag atggtgatcg
      781 gcgagaagac caccctggag gagatgggcg gggcggccat gcactgccgg gtgtcgggct
      841 gcggcgacct gctctgcgcc gacgaggacg aggcaatcca ggccgcccgc cgctacctcg
      901 gctacttccc cgggagctgg cggggcgacc cgccggccga ggccccgcgg gggccggcct
      961 accccgggcc ggtggctccc ttggtcccga ccgacgagtc gcagccgttc gacgtctacc
     1021 cgctggtcgc ggcggtggtc gacgaggaga gcttcttcga ggtcaagcgc gagttcgcgg
     1081 ccgaggtggt ctgcgggctg ggccggatcg agggccgggc cgtcggcatc gtcgccaacc
     1141 agccgctggt caagggcggg gtgctgttcg tcgactcggc cgacaaggtg gcccgcttcg
     1201 tctggctgtg cgacgccttc aacctgccgc tggtgttcct ggccgacgtg cccgggttca
     1261 tgatcgggac cgaggtcgag cgccagggca tcatccgcca cggggccaag ctgatcaccg
     1321 ccgtcagcga ggccaccgtg cccaagatct gcgtggtcgt gcgcaaggcg tacggggccg
     1381 ggctgtacgc catggccggg cccgggttcg agcccgacgc caccctggcc ctgccgacgg
     1441 cctcgatcgc cgtgatgggg ccggaggcgg ccgtcaacgc cgtctactac aacaagctgg
     1501 ccgccctccc cgaggccgag cggccggccg aggtcgagcg gctccagcgc gagtaccgcg
     1561 aggacgtcga cctggtccgc ctggccgccg acctggtcgt ggacgcggtc gtcgaccccg
     1621 aggacctccg cagccagctg gcccggcgtt tggccgccgc cgccggcaag gaccgcgagt
     1681 tcacccgccg gaggcacggg gtgcctccgg tatgaacgcc cggcccttcc ggtccgtgct
     1741 ggtggccaac cggggggaga tcgccgtgcg ggtgctgcgg gcctgccggg agctggggct
     1801 gaccgggatc gccgtccact ccgacccgga cgcgggggcc ctgcacgtgc gcgaggccga
     1861 ccgggccgtg gccatcggcg gctcggcggt ggccgagtcc tacctggacc aggacaggct
     1921 gctggccgcc gccgccgagg cgggggccga ggccgtccac cccggctacg ggctgctcag
     1981 cgagcacgcc ggcttcgccc gggccgtgac cggcgccggg ctggtctggg tcggcccccc
     2041 gcccgaggcc atcgcccaga tgggcgacaa gctggccgcc cggcgggcgg tgctggccgc
     2101 cggggtcgag ccggtgcccg gcacgaccga cccggtgacc gacccggccg aggtggtcgc
     2161 cttcggcgag gccaacggct ggccggtcgc ggtcaaggcg gccgccggcg ggggcggccg
     2221 cggcatccgc gtcgtcgccg gccccggtga cgccgccgag gccctggccg ccgccacccg
     2281 ggaggcgcag gccgccttcg gcgacggcgc ctgctacctg gagcgcttct tcccccggcc
     2341 ccgccacgtc gaggtccagg tgctggccga cacccacggc gccatcgtcc agctggggga
     2401 gcgggactgc tcggtgcagc gccgccacca gaagctggtc gaggaggcgc cctgccccgg
     2461 gctgccggcc gaggtcggcc agcggctgcg cacctgggcg gtggcggtgg ccaaggcctg
     2521 cggctacgtc ggcgccggga cggttgagtt cctctgggac ccggcctcgg gcggcgcctg
     2581 gttcctggag atgaacaccc gcatccaggt cgagcacccg atcaccgagc aggtcaccgg
     2641 gatcgacatc gtcgccgccc agctgcgggt ggcggccggc gagccgctcg gcctggccca
     2701 ggacgacgtg gccgtgcgcg gccacgccat cgagtgccgg gtcaacgccg aggacccggc
     2761 ccgcggcttc ctgcccaccc cgggcacgat caccgccctg cgctggccgg gcggccccgg
     2821 cgtccgggtc gacgccggct acgagcgcgg cgacaccgtg cccccgttct acgacgacct
     2881 gctgggcaag ctggtcgcct gggcgcccga ccgcgaccag gcgatcgcgc ggatgcgggc
     2941 cgccctcgac gagctggtcg tcgagggcgt cgccaccacc gccccggccc tggcccggat
     3001 gctggaccac gacgatttcc gggcggccag gcactggacg acctggctgg aggcggtcga
     3061 cctcggcggc ctcgccccgg cggcggcccc tgaggacggc cccgacttcc acgtcacggc
     3121 cggcggccgg acctggccgg tgcggctcca ccggcaccgt ccccgggcca ccggcggcgg
     3181 cgccggcggc ggggggctgg tggcctcgcc gctggccggg accctggcca ggctgacggt
     3241 caccgcaggt cagcgcgttg ccgaagggga gctgctggcc gtggtcgagg ccatgaagat
     3301 ggagacgccg ctgcttgctc cgttttctgg cattgtggtc ggggtaagat ccgcggttgg
     3361 cgggccggtt gcggccggcc agatcgtggt cgagctggag gccggaggac ggcgataggg
     3421 ggtcgtcatc gggttgtcgg aagcggggat gttggaggcg caggggtttc gagagttcag
     3481 cgggcacgcc gaccaggtcg cggcggcccg tcgcttcgtc gcctccgcga tccagggcgg
     3541 cgggcccgcc cgcgacgtca gccggctcct ggtcagcgag gccgccacca acgccgtcct
     3601 gcactcggcc accggtgacg gcggcagctt ctcggtcgcc tacctgatct ccgaccacct
     3661 gctccgggtc gaggtccacg acggcggcgg ccccagcgga ccgcgccgcc gcgtccacca
     3721 cctcgagtcc atgaccggcc gcggcctgga gctgttcgac gccctgtcgg accgctgggg
     3781 cgtggacggc agcccggacg gctggacggt ctggttcgag ctcgacctgg cggccgagga
     3841 agcccgcagc gacgccagcc gctagaactc cggctcgcgc ttctcgaccc aggcggcgat
     3901 gccctcgcgg cggtcgtcgg agaggacggc ccgccgccag gcctggtcct cgacctccat
     3961 ggccgcctcc tgggccaggt cggcgcccag atcgatggcc cacttggcca tgcggacggc
     4021 ggtcggggcg ttg
//
LOCUS       DASSWR010000374         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_127740,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000374 DASSWR010000000
VERSION     DASSWR010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127740"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="rplJ"
                     /locus_tag="VFO47_06190"
     CDS             complement(<1..587)
                     /gene="rplJ"
                     /locus_tag="VFO47_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET8788319.1"
                     /translation="MPNASNVAEVEAIKGRLEGSVAALLTEYRGLKVAELGELRASLR
                     GSSTEYRVLKNTLTSIAVREAGYEDLIELLQGPTAVAFVHGDPVQAAKDLAEFARTHP
                     ALIVKGGVMDGKVLGADEVRQLATLESREVLLARLAGLLQASAQQAVNLLAAPLRQVA
                     TMTAALRDKTPGDPNATAEGAQPEAQAEAEEAPAA"
     gene            967..1251
                     /locus_tag="VFO47_06195"
     CDS             967..1251
                     /locus_tag="VFO47_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788320.1"
                     /translation="MATPVIISLLVGLVAGGAFMAMWAANRIEEEGVRQFDRGFERGR
                     WLTDISVARAIEDQERTRRVGLLVAGTRQPSGPPARPPGQEPRPSLLVLP"
     gene            1326..1469
                     /locus_tag="VFO47_06200"
     CDS             1326..1469
                     /locus_tag="VFO47_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788321.1"
                     /translation="MPADLVAIATLVVLATLAPVTAWRRRLATRRFDAVYERNLLRRT
                     GRG"
     gene            complement(1507..2382)
                     /locus_tag="VFO47_06205"
     CDS             complement(1507..2382)
                     /locus_tag="VFO47_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788322.1"
                     /translation="MSRDAWELGWWAPVPPVLEPGELVDPGVHGALLSAVADGAPLDR
                     LGQHRLESLKAAGLATPDGRPRFPVAQAAQAKAVAETAGDLGRAMARLVAGEWSRLEV
                     EYESVHAALARPEPGGPAFLVVGGLLLDIGVRRLLRRQGLAAPPFGSAFVWLAEGEGA
                     AGGWFARVTGLPGRGSLIRFGRPAAPAFQLAAADPESAPALPAALEPALRELCEGLGW
                     SVVRLVEDALPALDPVRRRVPGADDEGAFLAWAYTLAVDEALDRLAARGLLTPPDTAV
                     TAVRVTDPALAGAAT"
     gene            complement(2443..3153)
                     /gene="rplA"
                     /locus_tag="VFO47_06210"
     CDS             complement(2443..3153)
                     /gene="rplA"
                     /locus_tag="VFO47_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET8788323.1"
                     /translation="MAGRKLAEAAAKIDRTRLYHPAEALELARETNPTKFDATVEVAF
                     RLGVDPRRADQMVRGTVSLPNGTGKTIRVAVFAEGEAARLAEEAGADVVGGKDLVERV
                     QGGFLDFDAAVAVPEMMGQVGRLGRILGPRGLMPNPRAGTVTPDVARAVSEIKGGKVE
                     YRVDRHGNLHLILGKTSFDARALVENYQVVLDEILRAKPAAAKGRYIKGITVATSMGP
                     GIKVDPGATRNLVPAASA"
     gene            complement(3222..3665)
                     /gene="rplK"
                     /locus_tag="VFO47_06215"
     CDS             complement(3222..3665)
                     /gene="rplK"
                     /locus_tag="VFO47_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET8788324.1"
                     /translation="MPPQAGRRRRVAAVLKIELTAGQATPAPPVGTALGPHAVNIMDF
                     CKQYNAATQAQAGNVVPVEITIYEDRSFSFVLKTPPAAVLLRKAAGIEKGSPEPHREK
                     VGTVTRAQVREIAETKMPDLNAIDVEGAMKIVEGTARSMGITVAS"
     gene            complement(3690..>3947)
                     /locus_tag="VFO47_06220"
     CDS             complement(3690..>3947)
                     /locus_tag="VFO47_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006552291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HET8788325.1"
                     /translation="GSGAKPTPLSEKEVAKILETKPVEKLKPRVEWQVGESVTVTSGP
                     FAQLPGSIAEINADQQKLKVLVSIFGRETPVELAFDQVQKL"
BASE COUNT          483 a         1445 c         1477 g          542 t
ORIGIN      
        1 tcggcggcgg gggcctcctc ggcctcggcc tgagcctcgg gctgggcgcc ctcggcggtc
       61 gcgttcgggt cgcccggggt cttgtccctg agggcggcgg tcatggtcgc cacctgccgg
      121 agcggggcgg cgagcaggtt cacggcctgc tgcgcggagg cctggagcag cccggccagc
      181 cgggccagca ggacctcgcg ggactcgagg gtggccagct ggcggacctc gtcggcgccc
      241 aggaccttgc cgtccatcac cccgcccttg acgatcaggg ccgggtgggt ccgggcgaac
      301 tcggccagat ccttggccgc ctgcacgggg tcgccgtgga cgaaggcgac cgcggtcggg
      361 ccctggagca gctcgatcag gtcctcgtag cccgcctcgc gcacggcgat gctggtcagg
      421 gtgttcttca gcacccggta ctcggtcgac gacccgcgca gcgacgcgcg cagctcgccc
      481 agctccgcga ccttgaggcc ccggtactcg gtgagcagcg cggccaccga gccttccagg
      541 cgtcccttga tggcctcgac ctcggcgacg ttgctcgcgt tcggcacggc ctcacctcca
      601 cacaaagaaa acggacggtc cccggaccgt ccgactggcg catgccacgc gtggaggcac
      661 ctcacggtgc tcgcctgcgc cggcgcccct ggacggggcc ttcgtgccat gcggcaaccg
      721 gacggtcttc ggctcgacga ggctgcgtgc gacctagacc acctagctgg gaagggatcc
      781 aggctgaccg ccaggtcgtg cgcggcctcg ttgacagcgg agagggtagc acaagggggc
      841 tgtggataac cgcgggcagg tcggcggccg tatcgatagg ttgctcccga ggcgccttgc
      901 tgtcacagct tccgaagccg tcgatctcat cacagttgac gatcgcagca ttcgaagggg
      961 gagtagatgg ccacgccggt catcatcagc ctgctcgtcg gcctcgtcgc cggcggagcg
     1021 ttcatggcca tgtgggccgc caaccgcatc gaggaggagg gcgtcaggca gttcgaccgc
     1081 gggttcgagc gcggccgctg gctgaccgac atcagcgtcg ccagggcgat cgaggaccag
     1141 gaacgaaccc gccgggtcgg cctgctcgtc gccggcaccc gccagccgag cggcccgccc
     1201 gcccggccac ccggtcagga gccccgcccg tcgctgctgg tcctccccta ggaccggcac
     1261 cggcccgagt cagtacccgc agcccgtcca ccgcggaccc tcggtccgcc gccgcaggag
     1321 ggaccatgcc cgccgacctg gtcgccatcg ccaccctcgt ggtgctggcc accctcgccc
     1381 ccgtcaccgc ctggcgccgc cgcctggcca cccgccgctt cgacgccgtc tacgagcgca
     1441 acctcctccg ccgcaccggc cgcggctgac cggccaggtc cgcgggctgg ccgccggggc
     1501 gacccgtcag gtggccgccc cggccagggc cgggtcggtg acacggaccg cggtgacggc
     1561 ggtgtcgggc ggggtgagga ggccgcgggc ggcgaggcgg tcgagggcct cgtcgacggc
     1621 cagggtgtag gcccaggcca ggaacgcgcc ctcgtcgtcg gcgcccggga cgcggcgccg
     1681 gaccgggtcg agggcgggga gggcgtcctc gaccaggcgg acgaccgacc acccgagccc
     1741 ctcgcagagc tcgcgcaggg ccggttcgag ggccgcgggc agggccgggg ccgactcggg
     1801 gtcggcggcg gcgagctgga acgccggcgc cgccgggcgc ccgaagcgga tcaggctgcc
     1861 gcgcccgggc aggccggtga cccgggcgaa ccagccgccg gcggcgccct cgccctcggc
     1921 cagccagacg aaggcgctgc cgaagggcgg cgcggccagg ccctggcggc gcagcaggcg
     1981 gcgcaccccg atgtcgagca ggagcccgcc cacgaccagg aaggcgggcc cgcccggctc
     2041 cgggcgggcc agggcggcgt gcacggactc gtactcgacc tcgagccggc tccactcgcc
     2101 cgccaccagg cgggccatgg cccggccgag gtcgccggcg gtctcggcga ccgccttggc
     2161 ctgggcggcc tgggcgaccg ggaagcgggg ccggccgtcc ggggtggcca gcccggccgc
     2221 cttcagcgac tccagccggt gctgccccag ccggtccagc ggcgccccgt cggccaccgc
     2281 cgacagcagg gccccgtgca cccccgggtc gaccagctcc ccgggttcca gcaccggcgg
     2341 caccggcgcc caccatccga gctcccaggc gtcccggctc acgggcaggc ccacccccgg
     2401 cgtctcgcgc ctctcgtccc gtcccgcatc gccgccgtcc ggtcaggcgc tggcggccgg
     2461 gaccaggttg cgggtggcgc cgggatcgac cttgatgccg gggcccatcg aggtggcgac
     2521 ggtgatgccc ttgatgtagc ggcctttggc cgcggccggc ttggcgcgga ggatctcgtc
     2581 gaggacgacc tggtagttct cgaccagggc cctggcgtcg aacgaggtct tgcccaggat
     2641 caggtggagg ttgccgtggc ggtcgacccg gtactcgacc ttgccgccct tgatctcgga
     2701 gacggccctg gccacgtccg gggtgacggt gccggcgcgc gggttgggca tcaggccgcg
     2761 ggggccgagg atgcggccca ggcggccgac ctggcccatc atctcgggca cggcgaccgc
     2821 ggcgtcgaag tcgaggaagc cgccctggac ccgctcgacc aggtccttgc cgccgaccac
     2881 gtcggccccg gcctcctcgg ccaggcgggc cgcctcgccc tcggcgaaca cggcgacccg
     2941 gatggtcttg ccggtgccgt tgggcagcga gacggtgccc cgcaccatct ggtcggcccg
     3001 gcgcgggtcg accccgagcc ggaaggcgac ctcgacggtg gcgtcgaact tggtcgggtt
     3061 ggtctcccgg gccagctcca gcgcctcggc cgggtggtag agccgggtgc ggtcgatctt
     3121 ggcggccgcc tcggccagct tcctgcctgc catcggttgt cctcctgtgg tcaacggacc
     3181 gctccgttgc ggccctccca cgaaccaggg aacgcgcctc gtcaggaggc gacggtgatg
     3241 cccatcgagc gggcggtgcc ctcgacgatc ttcatggcgc cttcgacgtc gatggcgttg
     3301 aggtcgggca tcttggtctc ggcgatctcg cggacctggg cccgggtgac cgtgccgacc
     3361 ttctcgcggt gcggctcggg cgagcccttc tcgatcccgg ccgccttgcg cagcagcacc
     3421 gcggccggcg gggtcttgag cacgaacgag aagctgcggt cctcgtagat ggtgatctcc
     3481 acggggacca cgttgccggc ctgggcctgg gtggccgcgt tgtactgctt gcagaagtcc
     3541 atgatgttca cggcgtgggg gccgagggcc gttcccaccg gcggcgccgg ggtggcctgg
     3601 ccggccgtga gctcgatctt gaggacggcc gccacgcggc ggcgacgacc tgcttggggt
     3661 ggcatggggt gtgtgcgctc cttgagggat tagagcttct gaacctggtc gaaggccagc
     3721 tcgaccgggg tctcgcggcc gaagatggag accaggacct tgagcttctg ctggtcggcg
     3781 ttgatctcgg cgatcgagcc gggcagctgg gcgaacgggc ccgaggtgac ggtgacggac
     3841 tcgccgacct gccactcgac ccgcggcttg agcttctcga ccggcttggt ctcgaggatc
     3901 ttggccactt ccttctcgga cagcggggtc ggcttggccc cggagcc
//
LOCUS       DASSWR010000375         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_918292,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000375 DASSWR010000000
VERSION     DASSWR010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918292"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1107
                     /locus_tag="VFO47_06225"
     CDS             196..1107
                     /locus_tag="VFO47_06225"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HET8788326.1"
                     /translation="MGILRLSHVDVRVPDLDLASAYYTEVMGLLEVERTATEVYLKCW
                     DEPDHHSVKLAYAPRVGLDLISFKVEADEDLAELESRVEGYGFPVRRVSRGESVGQGQ
                     SIRFETPSGQLMELVREVERVGGLLPKVNPPPFPPQELRGIAPPRIDHVLVTAEEVGE
                     ATRFYMEVLGFRLTEQLLDGNGHQIGVWMERSHSPHDLAVVTGANGGLHHFAFWLDDW
                     DHVRKAADILAYNGVQIDVGPTRHGITRGNTIYFFDPLGTRNEVFTGGYRPDPDFPTI
                     TWTEDNIGRAIFYYEGDLNDRFMKVHT"
     gene            1109..2020
                     /locus_tag="VFO47_06230"
     CDS             1109..2020
                     /locus_tag="VFO47_06230"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HET8788327.1"
                     /translation="MGILRLSHVEVRVPDLELATAYYTEVLGLLETGRDAERVFLKCW
                     DEHEHHSVILRYAATYGLEHMGFKVEHADDLERFEKRVEQDGGQVTRHARGELAPGHG
                     EAIRFQTPTGHRCELVHGMEKVGNLLPRTNPPPRPLGLVGIAPPRLDHIFVTAEDVDG
                     GTRFFREVLGFRLTEQILADDGHQLATWLEVSHTPHDIALVTGPNGGLHHFAFWLDDW
                     NEVRTAADILAYHGVPIDVGPTRHGATRGYAVYFFDAAGNRNEVFTGGYWVDPDHEVV
                     TWTEAEMGRAIFYYEGAVNQRFLTVHS"
     gene            2095..>2639
                     /locus_tag="VFO47_06235"
     CDS             2095..>2639
                     /locus_tag="VFO47_06235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8788328.1"
                     /translation="MGQGEEPALPRTERPDAPLYLASYLRRRARAAVPETATDPEAET
                     RADPATPLYLRRFRERRADPDAPDAPPPVWNGEPLEVTPAWAEITRTKEVVPETDAGA
                     FRVAAQVHIIRHGETQGYSTESGLTPLGGWQAHRRGFDLSKGVREGEQVQIVCADTNR
                     ARQTAEHLHRGLLDGLDLWRR"
BASE COUNT          403 a         1003 c          897 g          336 t
ORIGIN      
        1 ccacgaagtc ggccatggcc ttccgcatct gctcgggggc cggaggggtc cggggaaccc
       61 cagcggggtg ccccggtgaa ctgggaccca tcgtttcgcg accatagccc acgttcctcc
      121 atgcgggaag ggacgctatc ccgcggagcg ggacgggccg taccttctcc cgaagcggct
      181 gagcaaggag cgtgcatggg gatcctccgc ctgtcgcacg tggacgtccg cgtgcccgac
      241 ctggacctgg ccagcgccta ctacaccgag gtcatgggcc tgctcgaggt cgagcggacc
      301 gccaccgagg tctacctcaa gtgctgggac gagcccgacc accactcggt caagctcgcc
      361 tacgctcccc gggtcgggct ggacctgatc agcttcaagg tcgaggccga cgaggacctg
      421 gccgagctcg agtcgcgggt cgagggctac ggcttcccgg tgcgccgcgt gtcgcgcggc
      481 gagtcggtcg gccagggcca gtcgatccgc ttcgagaccc cctcggggca gctgatggag
      541 ctggtccgcg aggtcgagcg ggtcggcggg ctgctcccca aggtcaaccc gccgccgttc
      601 ccgccccaag agctccgcgg catcgccccg ccccggatcg accacgtcct ggtcacggcc
      661 gaggaggtgg gcgaggcgac ccgcttctac atggaggtgc tggggttccg cctgaccgag
      721 cagctgctgg acggcaacgg ccaccagatc ggcgtctgga tggagcgctc ccactcgccc
      781 cacgacctgg ccgtggtcac cggggccaac ggcggcctgc accacttcgc gttctggctg
      841 gacgactggg accacgtccg caaggcggcc gacatcctcg cctacaacgg cgtccagatc
      901 gacgtcggcc cgacccgcca cggcatcacc cgcggcaaca ccatctactt cttcgacccc
      961 ctcggcaccc gcaacgaggt cttcacgggc ggctaccggc ccgacccgga cttccccacc
     1021 atcacctgga ccgaggacaa catcggccgg gcgatcttct actacgaggg cgacctgaac
     1081 gaccggttca tgaaggtcca cacctgagat gggcatcctg cgcctgtccc acgtcgaggt
     1141 ccgggtgccc gacctggagc tggccaccgc ctactacacc gaggtgctgg ggctgctgga
     1201 gacgggccgg gacgccgagc gggtgttcct caagtgctgg gacgagcacg agcaccactc
     1261 ggtgatcctc cgctacgccg ccacctacgg gctggagcac atgggcttca aggtcgagca
     1321 cgccgacgac ctggagcggt tcgagaaacg ggtcgagcag gacggcggcc aggtgacccg
     1381 ccacgcccgc ggcgagctgg ctcccggcca cggcgaggcc atccgcttcc agaccccgac
     1441 cgggcaccgc tgcgagctgg tccacgggat ggagaaggtc ggcaacctgc tcccccgcac
     1501 gaacccgccg ccgcggccgc tggggctggt cgggatcgcc ccgccccgcc tcgaccacat
     1561 cttcgtcacc gccgaggacg tcgacggggg gacccgcttc ttccgggagg tgctggggtt
     1621 ccggctgacc gagcagatcc tggccgacga cggccaccag ctggccacct ggctggaggt
     1681 gtcccacacc ccgcacgaca tcgccctggt caccgggccc aacggcggcc tccaccattt
     1741 cgccttctgg ctggacgact ggaacgaggt ccgcaccgcc gccgacatcc tggcctacca
     1801 cggggtgccg atcgacgtcg gcccgacccg ccacggggcc acccgcggct acgccgtcta
     1861 cttcttcgac gccgccggca accgcaacga ggtgttcacc ggcggctact gggtcgaccc
     1921 cgaccacgag gtcgtcacct ggaccgaggc cgagatgggc cgggccatct tctactacga
     1981 gggcgccgtc aaccagcggt tcctgaccgt gcacagctga cccgagaagg gggcgagatg
     2041 gccgaggacc cgccgcggcc ggaggggttc ggggaacccc gagggggtgc cccgatgggt
     2101 cagggcgagg agccggccct gccgcggacc gagcggcccg acgcccccct gtacctggcc
     2161 agctacctgc gccgccgggc ccgggcggcc gtccccgaga cggccaccga ccccgaggcc
     2221 gagacccggg ccgacccggc cacgccgctg tacctgcggc ggttccggga acggcgggcc
     2281 gacccggacg ccccggacgc gcccccgccg gtctggaacg gcgagccgct ggaggtgacc
     2341 ccggcctggg ccgagatcac ccggaccaag gaggtcgtcc ccgagaccga cgccggcgcc
     2401 ttccgggtgg ccgcccaggt ccacatcatc cgccacggcg agacccaggg gtactcgacc
     2461 gagagcggcc tcacccccct gggcggctgg caggcccacc ggcgcggctt cgacctgtcc
     2521 aagggtgtcc gcgagggcga gcaggtccag atcgtctgcg ccgacaccaa ccgggcccgc
     2581 cagaccgccg agcacctcca ccgcggcctg ctcgacggcc tcgacctgtg gcgccgcga
//
LOCUS       DASSWR010000376         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_918305,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000376 DASSWR010000000
VERSION     DASSWR010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918305"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1680
                     /gene="leuC"
                     /locus_tag="VFO47_06240"
     CDS             259..1680
                     /gene="leuC"
                     /locus_tag="VFO47_06240"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET8788329.1"
                     /translation="MPAPRTLLDKVWDRHVVAEPPGLPAVLYVDLHLVHEVTSPQAFA
                     GLAARGLKVRRPDLTLATADHSVPTSDRSLPIVDELARAQVERLERNCAAHGIPLHGM
                     HSPHQGIVHVIGPELGATQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
                     LQRRPKSFEVRVEGRLAPGVTAKDVILALLAKIGVGGGTGHVFEYGGPAVRALSMEQR
                     MTICNMSIEGGARAGMVAPDDTTFEYVAGRPYAPRGEAWDRALADWRTLPGDDGAVFD
                     RTVTLDAGRLEPMVTYGTNPGMGLPITGRVPDPEHAGDPAARQALAKALTYMDLRPGE
                     PLLGRPVDVVFIGSCTNSRISDLRQAASLLRGRKVDPGVRVLVVPGSQDVKRQAEAEG
                     LAEVFRAAGAEWREAGCSMCLAMNGDQLAPGQYAVSTSNRNFEGRQGAGGRTFLASPL
                     TAAASALAGRIADARPLVETVEV"
     gene            1695..2309
                     /gene="leuD"
                     /locus_tag="VFO47_06245"
     CDS             1695..2309
                     /gene="leuD"
                     /locus_tag="VFO47_06245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET8788330.1"
                     /translation="MRPFTSTLVPLLVDNVDTDQIIPARFLKGTSKDGLGEGLFADWR
                     YDGDGRPREEFVLNRPEMAGRAVLLAGANFGSGSSREHATWALQAFGIKAVLAPLFAD
                     IFRNNALKNGLLPVELPAEAHAELAAAVEADPDAEVTVDLEAETVTLPGGRAVAFAVD
                     PFARQMLLDGTDELGYLLSHASAVAAYEDAHPARVDTTAPAWGG"
     gene            2302..>2836
                     /locus_tag="VFO47_06250"
     CDS             2302..>2836
                     /locus_tag="VFO47_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788331.1"
                     /translation="MAEAPGPAPPEDRPGPFPAALELLPAGPVALRLAAPASKSVTNR
                     ALLCAALADGASRLDGAAPSDDAEAMAAALTLLGARVDASSWTVTGTGGRLASPTRPL
                     DARLSGTTMRFLAAAATMTPGGATVTGAPPLLRRPGRRVHGRPVRPPDAAGRHRRARL
                     PAVARGRGRRLRGRQPLP"
BASE COUNT          345 a         1141 c         1004 g          346 t
ORIGIN      
        1 ctgcggccct ggggcagggg gccggccgtc gctcaggcga ccgccgaggc gggcaccccc
       61 caagggcgcg accaagctgg tcgtggtcgg ccaggaggcg gcccgatgca ggagaccttc
      121 ctgcccagcg gccagttccg cttcgccttc acccgctacc tctagacggc ccctcctcgg
      181 ttaccatggg cggctgacga cgacgtcccg cccatccccc gggtgcgacg acgcccggac
      241 cgacccgagg tgcctgccat gcccgcccca cgcaccctgc tcgacaaggt ctgggaccgc
      301 cacgtggtgg cggagcctcc cggcctgccg gccgtgctgt acgtcgacct ccacctcgtc
      361 cacgaggtga cctcgcccca ggccttcgcc ggcctggccg cccgcggcct caaggtccgc
      421 cggcccgacc tgaccctggc caccgccgac cactcggtgc cgacctcgga ccggtccctg
      481 ccgatcgtcg acgagctggc ccgggcccag gtggagcggc tggagcgcaa ctgcgccgcc
      541 cacggcatcc ccctgcacgg catgcacagc ccccaccagg gcatcgtcca cgtcatcggg
      601 cccgagctgg gcgccaccca gccgggcatg acgatcgtct gcggcgactc ccacacctcc
      661 acccacggcg ccttcggggc gctggccttc ggcatcggca ccagcgaggt cgagcacgtg
      721 ctggccaccc agaccctgct ccagcggcgg cccaagtcgt tcgaggtgcg ggtcgagggt
      781 cggctggccc ccggggtgac ggccaaggac gtgatcctgg ccctgctggc caagatcggc
      841 gtcggcggcg gcaccgggca cgtgttcgag tacggcggcc cggccgtccg cgcgctctcg
      901 atggagcagc ggatgaccat ctgcaacatg tcgatcgagg gcggggcccg ggcgggcatg
      961 gtcgcccccg acgacaccac cttcgagtac gtggccggcc ggccgtacgc cccccggggc
     1021 gaggcctggg accgggccct ggccgactgg cggaccctgc ccggcgacga cggcgccgtc
     1081 ttcgaccgca ccgtcaccct ggacgccggc cggctggagc cgatggtcac ctacggcacc
     1141 aacccgggca tgggcctgcc catcaccggc cgggtgcccg accccgagca cgccggcgac
     1201 ccggcggccc gtcaggccct ggccaaggcg ctcacctaca tggacctgcg cccgggcgag
     1261 cccctgctgg gccggccggt ggacgtggtc ttcatcggct cctgcaccaa ctcgcgcatc
     1321 tccgacctgc gccaggcggc ctcgctgctg cgcgggcgca aggtcgaccc gggggtgcgg
     1381 gtgctggtcg tgcctggctc ccaggacgtc aagcgccagg ccgaggccga gggcctggcc
     1441 gaggtgttcc gggccgccgg ggccgagtgg cgcgaggccg gctgctcgat gtgcctggcc
     1501 atgaacggcg accagctcgc ccccggccag tacgcggtgt cgacctccaa ccgtaacttc
     1561 gagggccgcc agggcgccgg tgggcgcacc ttcctggcca gccccctgac cgcggccgcc
     1621 tcggccctgg ccggccgcat cgccgacgcc cgcccgctgg tcgagacggt ggaggtgtag
     1681 cggtgccgga gccgctgcgg ccgttcacca gcacgctggt cccgctgctg gtcgacaacg
     1741 tcgacaccga ccagatcatc ccggcccggt tcctcaaggg gaccagcaag gacgggctgg
     1801 gcgaggggct gttcgccgac tggcgctacg acggcgacgg ccggcccagg gaggagttcg
     1861 tgctcaaccg gcccgagatg gccggccggg ccgtcctgct ggccggggcc aacttcgggt
     1921 cggggtcgtc gcgcgagcac gccacctggg ccctccaggc cttcggcatc aaggccgtgc
     1981 tggcgccgct gttcgccgac atcttccgca acaacgccct caagaacggg ctgctcccgg
     2041 tcgagctgcc ggccgaggcc cacgccgagc tggccgccgc cgtcgaggcc gacccggacg
     2101 ccgaggtcac cgtcgatctg gaggccgaga cggtcaccct gcccgggggg cgggcggtcg
     2161 ccttcgcggt cgacccgttc gcccgccaga tgctgctcga cggcaccgac gagctcggct
     2221 acctgctgtc gcacgcgtcc gcggtcgccg cctacgagga cgcccacccc gcccgggtcg
     2281 acaccaccgc ccccgcctgg ggtggctgag gcgccgggtc ccgccccacc ggaggaccgc
     2341 cccggcccgt tcccggccgc gctggagctg ctcccggccg gcccggtggc cctgcgcctg
     2401 gccgccccgg ccagcaagag cgtcaccaac cgggccctgc tctgcgccgc cctggccgac
     2461 ggcgccagcc ggctggatgg ggccgcgccc agcgacgacg ccgaggccat ggccgccgcc
     2521 ctcaccctgc tcggcgctcg ggtcgatgcg agcagctgga cggtgaccgg gaccgggggg
     2581 cggctggcca gcccgacccg gcccctggac gcccggctgt ccgggacgac catgcggttc
     2641 ctggccgcgg cggccaccat gaccccggga ggggcgaccg tcaccggggc cccgccgctg
     2701 ctccggcggc cgggccgtcg ggttcacggt cgacccgttc gcccgccaga tgctgctgga
     2761 cggcaccgac gagctcggct acctgctgtc gcacggggcc gcggtcgccg cctacgagga
     2821 cgccaacccc tccctg
//
LOCUS       DASSWR010000377         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_890586,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000377 DASSWR010000000
VERSION     DASSWR010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890586"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFO47_06255"
     CDS             <1..618
                     /locus_tag="VFO47_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8788332.1"
                     /translation="AQAGYKDTNGDSYVEGPGGAKIELDLIVPNGWTDWMESIRVVGE
                     SAKAVGIKVNTEFPEFQALVDARNKGDFDMLINNEKQLSNSPWEYYDYMFRLPVQDSQ
                     STANFGRYENKKAWDLVQQLDKTPTDDVAGMRKVTSQLQRIQLSELPVIPLWYNGAWA
                     QANTSVWTGWPSAKQGDSHLMPVTWRNYWELGSILMLAELQPAKQ"
     gene            657..1646
                     /locus_tag="VFO47_06260"
     CDS             657..1646
                     /locus_tag="VFO47_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788333.1"
                     /translation="MRQYFQRKLGIYALTFLVAVTVDWMIPRFMPGDPVQTMLARASL
                     RPEANQAMQAYYTSVFGLDVPLWQQYLNFWNRLLHGDLGVSIWLFPQPVTSIILRAIP
                     YTLALLIPAILLSWWAGNKFGAYAARRRWLDNAVLPVGYVLTATPYMWLGILLAWALA
                     TVAGVFPIAGGYSFDVEPSWSLSFGANLLRHWFLPFASLFLVGFGGWAIGMRNMIIYE
                     LEADYCHYLEALGAPQRLVRRYAFRNALLPQLTGLALALGVIVAGALVTEIVFSYPGL
                     GYLILQAINNQDFFLIQGAFLFIIIGVLLANFLIDVVYVLVDPRTRVGMRGGA"
     gene            1643..2569
                     /locus_tag="VFO47_06265"
     CDS             1643..2569
                     /locus_tag="VFO47_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788334.1"
                     /translation="MTVSDQEELAAATPAATAVTPVTVTGVRPGREALYFARRNRKLL
                     LGLAVVLAFLAVAAVGPLLTDHGPNEYVGPPSQRPSAQWWFGTTMFGQDVFAQFAHGL
                     RSTFLVGLLGGGLAAVIGMLVGFTAGYRGGVVDEVLNMVTNVVLVIPCLAVLLIIATY
                     LRVRGVVPEAIFIGVFSWPWVARAIRAQTFSLRSREFVDLARLSGAGGARIVLREIAP
                     NMSSYLFMTFILLFGGAVLTAAMLDFIGLGPTDGMSLGLMMNNAVLWSALPLGMWWWF
                     IPPGLGITMIVGALYIMNVGLDEVFNPKLREL"
BASE COUNT          377 a          940 c          837 g          442 t
ORIGIN      
        1 gcccaggccg gctacaagga caccaacggc gacagctacg tcgagggacc gggcggggcc
       61 aagatcgagc tcgacctcat cgtccccaac ggctggacgg actggatgga gtcgatccgg
      121 gtcgtcggcg agagcgccaa ggccgtcggc atcaaggtca acaccgagtt ccccgagttt
      181 caggcgctgg tcgacgcccg caacaagggc gacttcgaca tgctgatcaa caacgagaag
      241 cagctctcca actcgccctg ggagtactac gactacatgt tccggctgcc cgtccaggac
      301 tcccagagca cggccaactt cgggcgctac gagaacaaga aggcctggga cctggtccag
      361 cagctcgaca agaccccgac cgacgacgtg gccgggatgc gcaaggtcac ctcgcagctc
      421 cagcggatcc agctcagcga gctgccggtg atcccgctct ggtacaacgg cgcctgggcg
      481 caggccaaca cctcggtctg gaccggctgg ccgtcggcca agcagggcga cagccacctc
      541 atgccggtca cctggcggaa ctactgggag ctgggcagca tcctgatgct cgccgagctg
      601 cagccggcca agcagtaacg gcctgacgac gccgtcgcgg cgacgaggag caggggatgc
      661 gtcagtactt ccagcgcaag ctcgggatct acgcgctcac gttcctcgtc gccgtgacgg
      721 tcgactggat gatcccgcgg ttcatgcccg gcgacccggt gcagacgatg ctggcccgag
      781 cctcgctgcg ccccgaggcc aaccaggcca tgcaggccta ctacaccagc gtgttcggcc
      841 tcgacgtccc gctctggcag cagtacctca acttctggaa ccggctgctc cacggcgacc
      901 tcggggtcag catctggctg ttcccgcagc cggtgaccag catcatcctg cgggccatcc
      961 cctacaccct ggccctgctc atcccggcca tcctgctcag ctggtgggcg ggcaacaagt
     1021 tcggcgccta cgcggcccgc cgcaggtggc tcgacaacgc cgtcctgccg gtcggctacg
     1081 tgctgacggc cacgccctac atgtggctgg gcatcctgct cgcctgggcg ctggccacgg
     1141 tggcgggcgt cttcccgatc gcgggcggct acagcttcga cgtcgagccg tcgtggtcgc
     1201 tgtcgttcgg cgccaacctg ctgcggcact ggttcctgcc gttcgcctcg ctgttcctgg
     1261 tcggcttcgg cggctgggcg atcggcatgc gcaacatgat catctacgag ctggaggcgg
     1321 actactgcca ctacctggag gcgctgggcg cgccccagcg gctggtgcgc cgctacgcct
     1381 tccgcaacgc cctgctgccc cagctcaccg ggctggccct ggccctcggg gtcatcgtgg
     1441 ccggcgccct ggtcaccgag atcgtcttct cctacccggg cctgggctac ctcatcctgc
     1501 aggccatcaa caaccaggac ttcttcctca tccagggcgc cttcctgttc atcatcatcg
     1561 gggtcctgct ggccaacttc ctgatcgacg tcgtctacgt cctggtcgac ccgcggaccc
     1621 gggtcgggat gcgaggtggc gcatgaccgt ctccgatcag gaagagctcg ccgccgcgac
     1681 cccggccgcg accgcggtca ccccggtcac cgtcacagga gtccggcccg gccgcgaggc
     1741 cctctacttc gcccgccgca accgcaagct gctcctcggc ctggccgtgg tgctggcctt
     1801 cctggccgtg gccgccgtcg gccccctgct caccgaccac ggccccaacg agtacgtggg
     1861 cccgccgtcg cagcggccct cggcccagtg gtggttcggc accaccatgt tcggccagga
     1921 cgtgttcgcc cagttcgccc acggcctgcg ctccaccttc ctggtcgggc tgctgggggg
     1981 cgggctggcg gcggtcatcg ggatgctggt cggcttcacc gccggctacc ggggcggggt
     2041 cgtggacgag gtcctgaaca tggtcaccaa cgtcgtgctg gtcatcccct gcctggccgt
     2101 gctgctgatc atcgccacct acctgcgcgt ccggggcgtc gtccccgagg ccatcttcat
     2161 cggggtgttc tcctggccgt gggtggccag ggccatcagg gcccagacgt tctcgctgcg
     2221 gtcgcgggag ttcgtcgacc tggcccgcct cagcggcgcc ggcggggcca ggatcgtcct
     2281 gcgcgagatc gcccccaaca tgagctcgta cctgttcatg accttcatcc tgctcttcgg
     2341 gggggcggtc ctgaccgcgg ccatgctcga cttcatcggg ctcggcccga ccgacggcat
     2401 gtcgctcggg ctgatgatga acaacgccgt cctctggagc gccctgccgc tgggcatgtg
     2461 gtggtggttc atcccgccgg gtctcgggat caccatgatc gtgggggcgc tctacatcat
     2521 gaacgtcggc ctcgacgagg tcttcaaccc caagctgcgg gagctctgag ccagcggtga
     2581 gcctccgggt cgacaa
//
LOCUS       DASSWR010000378         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1314182, whole genome shotgun sequence.
ACCESSION   DASSWR010000378 DASSWR010000000
VERSION     DASSWR010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314182"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFO47_06270"
     CDS             complement(<1..629)
                     /locus_tag="VFO47_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788335.1"
                     /translation="MSVASVPTARGGGHPSAARAVAAGAPAGIVGGLAFGAAMAQLGV
                     LPTVATLVGLGSSRAGFAVHMAISAVVGAGFGLLVRRQRCGPSETVFLGAAYGALWWC
                     IGPLTLLPLALGQPPAWEVAAAQREFPSLLGHLLFGMGTALVFVVLHGPGDKGRAGHA
                     VGAGTLLRGLAAGGLAAAALAAVLENRAPAVAAGAAVGLAQAVLSPRSD"
     gene            complement(626..2116)
                     /locus_tag="VFO47_06275"
     CDS             complement(626..2116)
                     /locus_tag="VFO47_06275"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8788336.1"
                     /translation="MLVWAVLVLTLLPALQASMAALFPTLLLSLGAGGVVGPGVQAAL
                     RGGRARRLRLPAAAAAGLGRRRVVILGGGFGGVSAARRFEQLLPWTPWLDVTLVSQRN
                     YLLFTPMLAEVAAGAVEAGNVGVPLRAACPRTRFRRAEVLGVDPDRKVVHLRRGDALE
                     ALPYDQLVLALGAVPNFRDLPGVAGNALTLATLEDARRLRDHVLLRLEQADHEPDPAE
                     RRRWLTFVVAGGGFAGTEAVASLFDLVHAVLRYFPRIRASEPRFVLVHSRERILPELG
                     DRLAGYAGDKLVARGIELRLGVTVAGADPDGVELSDGERVAARTLVWAAGTRPSPVPL
                     GAPDLAWRRPAPMAGAVPVDRTLRVRGTDDIWAVGDCARIPDLSRRGATCPPTAQHAV
                     RQGRAVADNVVAALTGAEPEPFRFRGLGFLVPLGRQSAAAELRGMRFSGLPAWLLWRG
                     VYLWKLPGPQKRLRVLLDWTVELLFPRDIALTASERHPAPQIHPMK"
     gene            complement(2361..3047)
                     /locus_tag="VFO47_06280"
     CDS             complement(2361..3047)
                     /locus_tag="VFO47_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET8788337.1"
                     /translation="MFRRVTMAAVVAVVAAAMVAPAALAQDDYSTTQVEYGDDVYGGT
                     EGSTETEAATGGAQSTGAWTKFSAKLSGDNEVGGGDPDGSGTAAVKTRGTEVCYDLKW
                     TGVDVTMAHIHKGAAGTNGDVVVNFFANESPLDASSKSGCVQAKEAVVKGLTANPSNY
                     YVNLHSPEFPKGAIRGQTALLSGNGELAFTGGPMSKGLMLLGLCVIAAGSTLLAVGQR
                     RRVAYHRPRH"
     gene            complement(3096..>3645)
                     /locus_tag="VFO47_06285"
     CDS             complement(3096..>3645)
                     /locus_tag="VFO47_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788338.1"
                     /translation="ADPNGNVNNLPLNSGVQTGTVFNSVPYGEIPGNTPQVLIQRPGS
                     GRIIPLNRELTVRARFDNFEPGFFSNPQAEYGVGTQRLNAEGNVQGHNHACFQRVARD
                     GSVPAETCASFVVMSQEGDSDVLTGIAPPLTEPGRYRLCVDAAAGNHYPATRAFAQRG
                     GPVDCVRVLAMDFSQLRRANNG"
BASE COUNT          468 a         1303 c         1442 g          432 t
ORIGIN      
        1 tggtcggagc ggggcgacag gaccgcctgg gcgagcccga cggccgcccc ggcggccacc
       61 gccggggccc ggttctccag gaccgcggcc agcgccgcgg cggccagccc gccggcggcc
      121 agcccgcgca gcagcgtccc ggcgccgacc gcgtgcccgg cccgcccctt gtcgccgggg
      181 ccgtgcagga cgacgaacac cagcgccgtc cccatcccga acagcaggtg gccgagcagg
      241 ctggggaact cgcgctgggc ggcggccacc tcccaggccg gaggctggcc gagggccagc
      301 ggcagcaggg tcagcggccc gatgcaccac cacagggcgc cgtaggcggc gccgaggaac
      361 accgtctcgc tgggcccgca gcgctggcgc cggaccagca gcccgaagcc ggccccgacc
      421 accgccgaga tggccatgtg gacggcgaac ccggcccgcg acgagcccag cccgaccagc
      481 gtggccaccg tcggcagcac gcccagctgg gccatggcgg cgccgaaggc gagcccgccg
      541 acgatcccgg ccggggcgcc ggcggccacc gcccgcgcgg ccgaggggtg accgcccccc
      601 ctggcggtcg ggacgctggc cacgctcatt tcatggggtg gatctgggga gcggggtgcc
      661 gctcggaggc ggtgagggcg atgtccctgg ggaacagcag ctcgacggtc cagtcgagca
      721 ggacccgcag ccgcttctgc ggcccgggca gcttccacag gtagacgccg cgccacagca
      781 gccaggcggg caggccggag aagcgcatgc cgcgcagctc ggcggcggcg ctctggcggc
      841 ccagcggcac caggaacccc aggccgcgga agcggaacgg ctcgggctcg gcgccggtca
      901 gggcggcgac gacgttgtcg gccacggccc ggccctggcg gaccgcgtgc tgggccgttg
      961 gcgggcaggt ggcgccccgg cgcgagaggt cggggatgcg ggcgcagtcc cccaccgccc
     1021 agatgtcgtc ggtgccccgc acccgcaggg tccggtcgac cggcaccgcc ccggccatcg
     1081 gggcgggccg gcgccaggcc aggtcggggg cgccgagcgg gaccgggctg gggcgggtcc
     1141 cggcggccca gaccagggtg cgggccgcca cccgctcgcc gtcgctgagc tcgaccccgt
     1201 cggggtcggc gccggccacc gtgaccccga ggcgcagctc gatgccgcgg gcgaccagct
     1261 tgtcgccggc gtagccggcc agccggtcgc ccagctcggg caggatgcgc tcgcgcgagt
     1321 ggaccagcac gaagcggggc tcgctggcgc ggatgcgggg gaagtaacga agcacggcgt
     1381 ggaccaggtc gaacaggctg gccacggcct cggtgccggc gaacccgccg ccggcgacga
     1441 cgaaggtgag ccagcggcgg cgctcggccg ggtcgggctc gtggtcggcc tgctcgagcc
     1501 gcaacaggac gtggtcgcgc agccggcgcg cgtcctcaag ggtggccagg gtgagggcgt
     1561 tgccggcgac gccgggcagg tcgcggaagt tcggcaccgc gccgagggcg agcaccagct
     1621 ggtcgtaggg cagggcctcg agggcgtcgc cgcggcgcag gtggacgacc ttgcggtcgg
     1681 ggtcgacgcc gagcacctcg gcccgccgga agcgggtccg ggggcaggcc gcgcgcagcg
     1741 gcaccccgac gttgccggcc tcgaccgccc cggccgcgac ctcggccagc atcggggtga
     1801 agagaagata gttgcgctgg ctgaccaggg tcacgtccag ccacggggtc caggggagga
     1861 gctgctcgaa gcggcgggcg gcgctgacgc cgccgaaccc gccgccgagg atgacgaccc
     1921 ggcgccggcc cagaccggcc gcggccgccg ccggcagccg gagccggcgg gcccggccgc
     1981 cccgcagcgc ggcctgcacg cccgggccga ccacgccgcc ggcgcccagg ctcagcagca
     2041 gcgtcgggaa cagtgccgcc atcgacgcct gcagcgccgg cagcagcgtg aggacgagca
     2101 ctgcccagac cagcacaccg aggagcatcc cgtcgatgac gccgctgggc agtgaagatg
     2161 gcggcgggag gggcaggtct cccgtagcct gcccctccct gcctgcctgg cgtccggtgc
     2221 gagtggtccg cgtccccagg ttcgcgtcag tggtgccgac gccgccttcg gacctccgga
     2281 ccgtccgctc cctcctcgac ggacggccgt ccaccgggtc gccaaggtgc tgcatgtcgt
     2341 gggttgtcag ttgtcgtcgg tcagtggcgg ggacggtggt aggccacccg ccgccgctgg
     2401 ccaaccgcca gcagcgtcga cccggccgcg atcacgcaca ggcccagcag catcaggccc
     2461 ttggacatgg gaccgccggt gaaggccagc tcgccgttgc cgctcagcag ggcggtctgg
     2521 ccgcggatgg cgcccttggg gaactcgggc gagtggaggt tgacgtagta gttggacggg
     2581 ttggcggtca gccccttgac cacagcttcc ttggcctgga cgcagccgga cttgctgctg
     2641 gcgtccagcg ggctctcgtt ggcgaagaag ttgaccacca cgtcgccgtt ggtgccggcg
     2701 gcgcccttgt ggatgtgggc catggtcacg tcgaccccgg tccacttcag gtcgtagcag
     2761 acctcggtgc cgcgcgtctt gaccgccgcg gtgccgctgc cgtcggggtc gcccccgccg
     2821 acctcgttgt cgccggacag cttggccgag aacttggtcc aggcgccggt cgactgggcg
     2881 ccgccggtgg ccgcctcggt ctcggtgctg ccctcggtgc cgccgtagac gtcgtcaccg
     2941 tactcgacct gggtggtgct gtagtcgtcc tgggcgaggg cggccggcgc gaccatcgcg
     3001 gcggccacga cggcgaccac cgccgccatc gtcactctcc tgaacatgaa gggtgctcct
     3061 cctggggtgt cgtgtcggtg acggacgggt caggcctagc cgttgttggc ccggcggagc
     3121 tggctgaagt ccatggccag cacgcggacg cagtcgaccg ggccgccgcg ctgggcgaac
     3181 gcccgggtgg cggggtagtg gttgccggcg gcggcgtcca cgcagaggcg gtagcggccg
     3241 ggctcggtga ggggcggcgc gatgccggtg aggacgtcgg agtcgccctc ctgggacatg
     3301 accacgaacg aggcgcaggt ctcggcgggc acgctgccgt cacgggcgac ccgctggaag
     3361 caggcgtggt tgtggccctg gacgttgccc tcggcgttca gccgctgggt cccgacgccg
     3421 tactcggcct gggggttgga gaagaacccg ggctcgaagt tgtcgaagcg ggcccggacg
     3481 gtcagctccc ggttgagcgg gatgatgcgg ccgctgcccg gacgctggat cagcacctgc
     3541 ggggtgttgc cggggatctc gccgtagggg accgagttga agaccgtgcc ggtctggacc
     3601 ccgctgttga ggggcaggtt gttgacgttg ccgttggggt cggcg
//
LOCUS       DASSWR010000379         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_947074,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000379 DASSWR010000000
VERSION     DASSWR010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947074"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="VFO47_06290"
     CDS             <1..1663
                     /locus_tag="VFO47_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8788339.1"
                     /translation="TTLDEYRKHIEKDAALERRFQPVLVGEPSVQDTIAILRGLKERY
                     EVHHGVRIQDSALVAAAVLSDRYVTGRFLPDKAIDLVDEAASRLRIEIDSMPTEVDVL
                     ERRARQLEIERAALKKEKDEVSRERLAKLEEELATLQETLTGMKAHWEGEKEAIGAIR
                     QINARIEEVKGEADRATRDGALERAAELGYGVLPGLERDLAEANRRLTELQADRRMLK
                     EEVDEEDVAKVVSAWTGIPVTRLVQGEMAKLLRLEDELHLRVVGQDDAVQAVADAIRR
                     SRAGLSEPGRPIGSFLFLGPTGVGKTELARALAALLFDDERAMVRIDMSEYGEKHSVA
                     RLVGAPPGYVGYDEGGQLTEAVRRRPYAVVLLDEVEKAHPDVFNVLLQVMDDGRLTDG
                     QGRTVDFANVVLIMTSNLGSSLLTDIVEPEEVSRERVMAAVRGAFKPEFLNRLDEILV
                     FHRLGRDDLARIVDLQVARLAGRLADRRLTLELTPAAKAWLADRGYDPAYGARPLRRL
                     IAKAVGDELARRLLAGELTEGDTVVVDAEDDKLTFTRRQPSAVAS"
     gene            1702..1995
                     /locus_tag="VFO47_06295"
     CDS             1702..1995
                     /locus_tag="VFO47_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788340.1"
                     /translation="MRRPPPGYRRGARPIYPGVFKMLIYGFILWGLSLLGIALPFHLP
                     RWLFVVLEVVVIWMLWRGVKAARRATHNQVRLFSAQENWANAFALQALILFLW"
     gene            complement(1976..>2828)
                     /locus_tag="VFO47_06300"
     CDS             complement(1976..>2828)
                     /locus_tag="VFO47_06300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8788341.1"
                     /translation="VLDARLVEAATAAGAELHRAGVRRLEQQDGMVVVDGKLAARVVV
                     GADGANSTVRRLLGAPPNPDRALAIAVRGYAPAPAGRPEQLIAWVAEGWPAYVWSFPT
                     GTGVANVGYGLLRSRFHGDRAELHRRLHDLLPEADPDPASLRAHHLPFSSFRPPPGKS
                     RVLLAGDAASLVNPLSGEGIYYALASGRLAARAALTDPDDALATYRRLLRTALGRHLR
                     HASVLARVIHAPALAEAGLGAAASSQRLFDTLVELGLGQARITPRLLAALPGGLLRAR
                     AATRGTG"
BASE COUNT          403 a         1000 c         1073 g          352 t
ORIGIN      
        1 gaccaccctc gacgagtacc gcaagcacat cgagaaggac gccgccctgg agcggcggtt
       61 ccagccggtc ctggtcgggg agcccagcgt ccaggacacc atcgccatcc tgcgcggcct
      121 caaggagcgc tacgaggtcc accacggcgt ccgcatccag gactcggccc tggtcgcggc
      181 ggcggtgctg tcggaccggt acgtgaccgg ccggttcctg cccgacaagg ccatcgacct
      241 ggtcgacgag gccgcctcgc ggctgcgcat cgagatcgac tcgatgccga ccgaggtcga
      301 cgtgctggag cgccgggccc gccagctcga gatcgagcgg gccgcgctca agaaggagaa
      361 ggacgaggtg tcgcgggagc ggctggccaa gctcgaggag gagctggcca ccctgcagga
      421 gaccctcacc ggcatgaagg cccactggga gggcgagaag gaggccatcg gggccatccg
      481 ccagatcaac gcccgcatcg aggaggtcaa gggcgaggcc gaccgggcca cccgcgacgg
      541 cgccctggag cgggcggccg agctgggcta cggcgtcctg cccggcctgg agcgcgacct
      601 ggcagaggcc aatcgacgcc tgaccgagct gcaggcagac cgaaggatgc tgaaagaaga
      661 agtggacgag gaggacgtgg ccaaggtcgt gtccgcctgg accggcatcc ccgtgacccg
      721 gctcgtccag ggcgagatgg ccaagctgct gcggctcgag gacgagctcc acctccgggt
      781 ggtcggccag gacgacgccg tccaggcggt cgccgacgcc atccggcgct cgcgggccgg
      841 gctgtcggag ccgggccggc cgatcggcag cttcctgttc ctcggcccca ccggggtcgg
      901 caagacggag ctggcccggg ccctggccgc gctgctgttc gacgacgagc gggccatggt
      961 ccgcatcgac atgagcgagt acggcgagaa gcactcggtg gcccggctgg tcggggcgcc
     1021 ccccggctac gtcggctacg acgagggcgg ccagctcacc gaggcggtgc ggcgccggcc
     1081 gtacgcggtg gtgctgctgg acgaggtcga gaaggcccac ccggacgtgt tcaacgtgct
     1141 cctccaggtg atggacgacg gccgcctgac cgacggccag ggccgcaccg tcgacttcgc
     1201 caacgtggtc ctgatcatga cctccaacct cgggtcgtcg ctgctcaccg acatcgtcga
     1261 gcccgaggag gtcagccgcg agcgggtcat ggccgcggtc cggggggcgt tcaagcccga
     1321 gttcctcaac cgcctggacg agatcctggt cttccaccgg cttggccgcg acgacctggc
     1381 ccggatcgtc gacctccagg tggcgaggct ggccggccgc ctggccgacc gccgcctcac
     1441 ccttgagctg accccggccg ccaaggcgtg gctggccgac cgcggctacg accccgccta
     1501 cggcgcccgc ccgctgcgga ggctgatcgc caaggccgtc ggcgacgagc tggccaggcg
     1561 cctgctcgcc ggcgagctga ccgagggcga cacggtcgtg gtcgacgccg aggacgacaa
     1621 gctgaccttc acccgtcgcc agccctcggc ggtggcctcg tgagggttcc ggtggttgtc
     1681 cacagaccct agactgcggg tatgcgccga ccgccgcccg gctaccgcag gggagcccgg
     1741 ccgatctacc cgggcgtctt caagatgctc atctacgggt tcatcctctg ggggctgtcc
     1801 ctgctgggca tcgcgctgcc cttccacctg ccgcggtggc tgttcgtggt gctcgaggtc
     1861 gtggtgatct ggatgctctg gcggggcgtc aaggcggcca ggcgcgccac ccacaaccag
     1921 gtgcggctgt tcagcgctca ggagaactgg gccaacgcgt tcgccctgca ggccctcatc
     1981 ctgttcctct ggtagcggcg cgggcccgca gcaggccgcc cgggagggcg gccagcagcc
     2041 gcggcgtgat cctggcctgg ccgaggccga gctcgaccag ggtgtcgaac agccgctggg
     2101 agctggcggc ggcgccgagg ccggcctcgg ccagggctgg ggcgtggatc accctggcca
     2161 ggaccgaagc atggcgcaaa tggcggccga gggccgtcct gagcaggcgc cggtaggtgg
     2221 ccagggcgtc gtccgggtcg gtcagggcgg ccctggcggc caggcggccc gaggccaggg
     2281 cgtagtagat gccctcgccc gacagcgggt tgaccagcga ggcggcgtcg ccggcgagca
     2341 ggacccggct cttgccgggc ggcggccgga acgacgagaa cggcaggtgg tgggcgcgca
     2401 ggctggccgg gtcggggtcg gcctcgggga gcaggtcgtg caggcgccgg tggagctcgg
     2461 cccggtcgcc gtggaagcgg ctgcgcagca gcccgtagcc gacgttggcc accccggtgc
     2521 cggtcgggaa cgaccagaca taggcaggcc agccctcggc cacccaggcg atcagctgct
     2581 cgggacggcc cgccggggcg ggcgcgtagc cgcgcacggc gatggccagg gcccggtccg
     2641 ggttgggcgg ggcgcccagc agccgccgga cggtcgagtt ggccccgtcg gccccgacca
     2701 ccacccgggc ggccagcttg ccgtcgacca ccaccatccc gtcctgctgc tccagacggc
     2761 ggacgccggc ccggtgcagc tcggctccgg cggccgtggc cgcctcgacc aggcgggcgt
     2821 cgagcacg
//
LOCUS       DASSWR010000380         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_918470,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000380 DASSWR010000000
VERSION     DASSWR010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918470"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1124
                     /locus_tag="VFO47_06305"
     CDS             114..1124
                     /locus_tag="VFO47_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8788342.1"
                     /translation="MTGPPTLEQVAALAGVSRATVSRVVNGSPKVSPVVRAQVERAVA
                     KLGYVPNRAARSLVTRRADSIALVVSEPHARFLSEPFFAGMVRGVSAALAETGVQLVL
                     LIAHDPPDRGRLERYVVGGHVDGVLLASLHGDDPLPGILDRAGVPAVLVGRPPGPTPA
                     TYVDADNRGGARKAVDHLVARGRRTIATISGPLDMAVGLDRLDGWRDGLAAAGLDGAG
                     DLVEAGDFTEEGGAAAMGRLLARPGRPVDAVFAASDLMAAGALRALRAAGRRVPEDVA
                     VVGFEDSAMARYAQPPLTTVRQPIEEMGRQATRLLLARAAGEPTGMHLILDVELVVRA
                     SA"
     gene            complement(1129..1842)
                     /locus_tag="VFO47_06310"
     CDS             complement(1129..1842)
                     /locus_tag="VFO47_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788343.1"
                     /translation="MTETTARSPRRDRVRQATVEEIKAVARAQMTAEGTAGVNLRAIA
                     REMGMTAPALYRYFASRDDLVTALVVDAYDALADAMEAAVEAAGPGRHAERVRAAFAA
                     FRAWGLAHPTEFALIFGSPIPGYAAPETTRPAGLRYTDLLGRLLADAWADGALDPDRI
                     DLRVPPALARQVEAFQRRRGGPALPAPVLAFGLGAWARLHGLVTLEVFGHLAPAIGDG
                     AALFEQELEAIVAQSGLRG"
     gene            1908..>2429
                     /locus_tag="VFO47_06315"
     CDS             1908..>2429
                     /locus_tag="VFO47_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8788344.1"
                     /translation="MTADLHVVVGASGGTGGALVRELARRGLPVRAVNRAGDASVPAG
                     VERLAADVTTPEGAKAAAAGAAVVYHCAQPPYTDWPAAFPPLTEAVAEGAAAAGAKLV
                     LADNLYLYGPHAGPLTEDLPAAATGPKGRVRAEMAARLLAMHRQGRLRVALGRASNYY
                     GPGGRNSVTGERLF"
BASE COUNT          287 a          945 c          917 g          280 t
ORIGIN      
        1 atcgtctacg tggacttccc gacccagcgc cggctgccca aggacagcgc cctctggtac
       61 cgggaggcga tcgcccgtgg cggcctggat ggcaccccga acggtgacgt gaggtgaccg
      121 gccccccgac cctggagcag gtggcggcgc tggccggggt ctcaagggcc accgtctccc
      181 gggtggtcaa cggctcgccc aaggtcagcc cggtcgtcag ggcccaggtc gagcgggccg
      241 tggccaagct cggctacgtg cccaaccggg ccgcccgcag cctggtcacc cggcgggccg
      301 actcgatcgc gctggtcgtc tccgagccgc acgcgcgctt cctctccgag cccttcttcg
      361 ccggcatggt ccgcggggtc agcgccgccc tggccgagac cggcgtccag ctggtgctgc
      421 tgatcgccca cgaccccccc gatcgtgggc ggctggagcg gtacgtggtc ggcggccacg
      481 tcgacggggt gctgctggcc tccctgcacg gcgacgaccc cctgcccggc atcctggacc
      541 gggccggcgt gcccgccgtc ctggtcggcc gcccgcccgg ccccaccccg gcgacctacg
      601 tggacgccga caaccgcggc ggggcccgca aggcggtcga ccacctggtc gcgcggggcc
      661 gccgcaccat cgccaccatc agcggccccc tggacatggc ggtcgggctg gaccgcctgg
      721 acggctggcg ggacgggctg gccgcggccg ggctggacgg ggccggcgac ctggtcgagg
      781 ccggcgactt caccgaggag ggcggggccg cggccatggg ccggctgctg gcccggccgg
      841 gccgcccggt cgacgccgtg ttcgccgcct ccgacctgat ggccgccggg gccctgcgag
      901 ccctgcgcgc ggcgggccgg cgggtccccg aggacgtggc cgtggtcggg ttcgaggact
      961 cggcgatggc ccgctacgcc cagccgcccc tgaccaccgt gcgccagccg atcgaggaga
     1021 tgggccgcca ggcgacccgc ctgctcctgg ccagggcggc cggcgagccc accggcatgc
     1081 acctgatcct ggacgtggag ctggtcgtcc gggcctcggc ctgagtcgtc agccgcggag
     1141 gcccgactgg gccacgatcg cctccagctc ctgctcgaac agggcggcgc cgtcgccgat
     1201 ggccggggcc aggtggccga agacctccag ggtgaccagg ccgtggaggc gggcccaggc
     1261 gcccaggccg aaggccagca ccggggccgg cagggcgggg ccgccgcgcc gccgctggaa
     1321 cgcctcgacc tggcgggcca gcgccggcgg cacccgcaga tcgatccggt ccgggtcgag
     1381 ggcgccgtcg gcccaggcgt cggccagcag ccggcccagc aggtcggtgt agcgcagccc
     1441 ggccgggcgg gtcgtctcgg gagcggcgta gccggggatg gggctgccga agatcagcgc
     1501 gaactcggtc gggtgggcga gcccccaggc ccggaaggcc gcgaacgcgg cccgcacccg
     1561 ctcggcgtgg cggccaggcc cggccgcctc gacggccgcc tccatggcgt cggccagcgc
     1621 gtcgtaggcg tcgacgacca gcgccgtgac caggtcgtcg cgggaggcga agtagcggta
     1681 gagggcgggg gcggtcatgc ccatctcccg ggcgatggcc cgaagattga ccccggccgt
     1741 gccctcggcg gtcatctgcg cccgggccac cgccttgatc tcctccacgg tggcctggcg
     1801 gacccggtcc cgccgcggcg accgcgccgt cgtctccgtc atgttgacag catacaatgt
     1861 agttactact gttaacaaca gtgaacgtta ttcagaagga gcggagcatg acagcggatc
     1921 tccacgtcgt cgtcggggcc agcggcggga ccggcggcgc cctcgtccgg gagctggccc
     1981 ggcggggcct gccggtgcgg gccgtcaacc gggcggggga cgcctccgtc ccggccgggg
     2041 tcgaacggct ggccgccgac gtgaccaccc ccgagggcgc caaggcggcc gccgccgggg
     2101 cggccgtcgt ctaccactgc gcccagccgc cctacaccga ctggccggcc gccttcccgc
     2161 ccctcaccga ggcggtcgcc gagggcgcgg ccgccgccgg ggccaagctg gtcctggccg
     2221 acaacctcta cctgtacggg ccccacgccg gtcccctcac cgaggacctc ccggccgccg
     2281 ccaccggacc caagggccgg gtccgggccg agatggccgc gcgcctcctg gccatgcacc
     2341 gccagggccg gctccgggtc gccctcggcc gggcgtccaa ctactacggc cccggcggcc
     2401 gcaacagcgt caccggcgag cggctgttc
//
LOCUS       DASSWR010000381         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1173640, whole genome shotgun sequence.
ACCESSION   DASSWR010000381 DASSWR010000000
VERSION     DASSWR010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173640"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /gene="dxs"
                     /locus_tag="VFO47_06320"
     CDS             complement(<1..1493)
                     /gene="dxs"
                     /locus_tag="VFO47_06320"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET8788345.1"
                     /translation="MLESIKQPSDLHSLSGDELTELAAEIRDFLIRAVAVRGGHLGPN
                     LGVVELTIALHRALDSPVDRIVWDTGHQAYVHKLLTGRQDDFAGLRTMGGLSGYPSRA
                     ESPHDVIENSHASTALGYALGLAEARRAGQGEGRVVAVVGDGSLTGGVALEALNLIGH
                     RKPDLLVILNDNGRSYAPTVGGLAGHLAPFRLHPGYEALKKGVEETFGRVPLVGEGMV
                     EAAKRVKEGAKALVAPRVVFEDLGWQYAGPVDGHDLASLEKAIDHAKRVPGPVLLHVI
                     TQKGRGYPPAEEHEEDNLHSLGAFDPATGRALPKSGDEVQFTHVFGQALLDEARRHPE
                     LVAISAAMLGPTNLTVMQREFPERVYDVGIAEQVGVTMAAGMALQGLRPVCAIYSTFL
                     QRAFDQVMMDVALHRLPVIFVLDRSGITGEDGSSHHGVYDVTWLREVPNLVIASPRDG
                     NELRRALATAIAHTAGPFAIRFPKSTTPKLTVSGPVRKVKLGAWDVRT"
     gene            complement(1547..>2151)
                     /locus_tag="VFO47_06325"
     CDS             complement(1547..>2151)
                     /locus_tag="VFO47_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET8788346.1"
                     /translation="LARAGVDPDLVTLAALWVALVAGWTATLGPGWAAAAGGLTLLAG
                     VADSVDGAVAVMQRRTSAFGFVWDSTADRLADLALIAGPVALVATEADPGWGTVAVAA
                     GAAAAGLLLLLEYVRARAQAGQVAAAWTLATPGERPTRVIVLGLAGLAVGAAQLGGDG
                     LAGAALDAGYPLALAALALLEAVGCVQLLLAVRHASSRLR"
BASE COUNT          281 a          784 c          820 g          266 t
ORIGIN      
        1 gtgcgcacgt cccaggcgcc cagcttgacc ttgcggaccg ggccggacac cgtcagcttg
       61 ggcgtggtcg acttggggaa gcggatggcg aacggcccgg cggtgtgggc gatcgcggtg
      121 gccagggcgc ggcgcagctc gttgccgtcg cggggcgagg cgatgaccag gttggggacc
      181 tcccgcagcc aggtgacgtc gtagaccccg tggtgggagg agccgtcctc gccggtgatg
      241 cccgagcggt cgagcacgaa gatcaccggc agccggtgga gggcgacgtc catcatcacc
      301 tggtcgaagg cccgctgcag gaacgtcgag tagatggcgc agaccggccg cagcccctgg
      361 agggccatgc cggcggccat ggtcaccccg acctgctcgg cgatcccgac gtcgtagacc
      421 cgctcgggga actcgcgctg catcaccgtg aggttggtcg ggccgagcat ggcggcggag
      481 atcgcgacca gctccgggtg gcggcgggcc tcgtccagca gggcctggcc gaacacgtgg
      541 gtgaactgga cctcgtcccc ggacttgggc agggcccgcc cggtcgcggg gtcgaaggcg
      601 ccgagggagt ggaggttgtc ctcctcgtgc tcctcggccg gcgggtagcc gcggcccttc
      661 tgggtgatca cgtggagcag gaccgggccc ggcacccgct tggcgtggtc gatggccttc
      721 tccagggagg ccaggtcgtg gccgtcgacc gggccggcgt actgccagcc caggtcctcg
      781 aacaccaccc ggggcgcgac cagggccttg gccccctcct tgacccgctt ggccgcctcg
      841 accatgccct cgccgaccag cggtacccgg ccgaacgtct cctcgacgcc cttcttcagg
      901 gcctcgtagc cggggtggag gcggaacggg gccaggtggc cggccaggcc gccgacggtg
      961 ggggcgtagc tgcggccgtt gtcgttgagg atcaccagca ggtcgggctt gcggtggccg
     1021 atcaggttga gggcctccag ggcgaccccg ccggtgaggg agccgtcgcc gaccaccgcc
     1081 accacccggc cctcgccctg gccggcccgg cgcgcctcgg ccaggccgag ggcgtagccg
     1141 agcgcggtcg aggcgtggga gttctcgatc acgtcgtggg ggctctcggc ccgcgacggg
     1201 tagccggaga gcccgcccat ggtgcgcagg ccggcgaagt cgtcctggcg gccggtgagc
     1261 agcttgtgga cgtacgcctg gtggccggtg tcccagacga tccggtcgac gggcgagtcc
     1321 agggcccggt ggagggcgat ggtcagctcg acgacgccga ggttggggcc gaggtggccg
     1381 ccgcggacgg ccaccgcccg gatgaggaag tcccggatct cggcggccag ctcggtgagc
     1441 tcgtcgccgc tgagggagtg taggtcgctt ggttgcttga tcgattccag catggcggtt
     1501 cgaggatatg gggtttccgg cgttcgtgcc tcctgagcgc ctgagcctac cgcagccggg
     1561 aagaagcgtg gcgcacggcc agcaggagct ggacgcagcc gacggcctcg agcagggcca
     1621 gggcggccag ggccaggggg tagccggcgt ccagggccgc cccggccagg ccgtcgccac
     1681 ccagctgggc cgcgccaacg gccaggccgg ccagcccgag cacgatcacc cgggtcggcc
     1741 gctcgcccgg ggtggccagc gtccaggccg ccgccacctg cccggcctgg gcccgcgccc
     1801 gcacgtactc gagcagcagc agcaacccgg ccgcggccgc cccggcggct accgcgaccg
     1861 tcccccagcc gggatcggcc tcggtcgcga ccagcgccac cggcccggcg atcagggcca
     1921 ggtcggccag ccggtcggcc gtcgagtccc acacgaaccc gaaggcgctg gtgcggcgct
     1981 gcatgaccgc caccgccccg tcgaccgagt ccgccacccc ggccagcagg gtgagccccc
     2041 ccgcggccgc cgcccacccc ggcccgagcg tggccgtcca cccggccacc agggccaccc
     2101 acagcgccgc cagcgtcacc aggtcgggat ccacccccgc cctggccagc g
//
LOCUS       DASSWR010000382         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_833763,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000382 DASSWR010000000
VERSION     DASSWR010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833763"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFO47_06330"
     CDS             <1..797
                     /locus_tag="VFO47_06330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8788347.1"
                     /translation="AEPVIVETDQSTGFRVSVSQLEAAATDRTKLLVFVSPSNPTGAV
                     YPPDEVEAIGRWAAGRGLWVLTDEIYEHLVYGQAEFTSMPVAVPELADRCVVVNGVAK
                     TYAMTGWRVGWLIGPPDVVKAAINLQSHATSNVANVSQAAALAAVSGDLVAVKEMRTA
                     FDRRRRKMLELLRAIPGVTCVEPEGAFYAFPSVEAVLGRELRGQRPATSMELADLILE
                     EAKVAIVPGEAFGAPGYARLSYALGDDDLVEGVTRIAKLLAEARPA"
     gene            complement(1250..1690)
                     /locus_tag="VFO47_06335"
                     /pseudo
     CDS             complement(1250..1690)
                     /locus_tag="VFO47_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            complement(1742..>2589)
                     /locus_tag="VFO47_06340"
     CDS             complement(1742..>2589)
                     /locus_tag="VFO47_06340"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET8788348.1"
                     /translation="EAAGKELAVRPEQGVGGVDVVFPILHGPFGEDGTVQGLFELADL
                     PYVGSGVLASALAMDKAMAKVVLAQAGLPQAPYLVVPERDWRADPGRVAAEVAGRLAY
                     PVFTKPARLGSSIGISKVKTPDGLAAGLAAAFAHDPKALVEQGVAARELECGVLGNDA
                     PEASVVGEVIPGHEFYDFEAKYLDESLKLEIPAQVPDAVRARVRELSLRAFQALDCEG
                     FARVDFFYEEATGRVLLNEVNTIPGFTPKSMFPMLWAASGVAYPDLVARLVDLAVERH
                     ADRRR"
BASE COUNT          352 a          961 c          932 g          344 t
ORIGIN      
        1 gggccgagcc ggtgatcgtg gagaccgacc agtcgaccgg cttccgggtc tcggtctccc
       61 agctcgaggc ggccgccacc gaccggacca agctgctggt gttcgtctcc ccctccaacc
      121 cgacgggcgc cgtctacccg cccgacgagg tcgaggccat cggccgctgg gcggccggcc
      181 gcggcctgtg ggtgctgacc gacgagatct acgagcacct ggtctacggg caggccgagt
      241 tcacctccat gccggtggcg gtgccggagc tggccgaccg ctgcgtggtc gtcaacgggg
      301 tggccaagac ctacgccatg accggctggc gggtcggctg gctgatcggc ccccctgacg
      361 tggtcaaggc ggccatcaac ctccagagcc acgccacctc gaacgtggcc aacgtctccc
      421 aggcggccgc cctggccgcc gtctccgggg acctggtggc ggtgaaggag atgcgcaccg
      481 ccttcgaccg gcggcgccgc aagatgctgg agctgctccg ggccatcccc ggcgtgacct
      541 gcgtcgagcc ggagggcgcc ttctacgcct tcccgtcggt cgaggcggtg ctgggccggg
      601 agctgcgcgg ccagcgcccg gccaccagca tggagctggc cgacctgatc ctggaggagg
      661 ccaaggtggc gatcgtgccc ggcgaggcct tcggcgcccc cggctacgcc cgcctgtcct
      721 acgccctcgg cgacgacgac ctggtcgagg gggtcacccg catcgccaag ctgctggccg
      781 aagcccgccc ggcctaggcg gccagctcga tctcgacgac caccaggggg ctgtcggtct
      841 gctcggggtc gccgacccgc agggtgatga cccgggccgc ctccgggggg gctggccgga
      901 agccgagctg gacgatgtgg agcaggcggc cgccgccggt ggccatgccc atcagctggt
      961 agtcggtgcc ggcgtcgtcg ctgacctgcc agtcgaagtc gaccaggtgc tgcgcgcccg
     1021 gctgctcctc gggcagcagg acgtagcgca ggtccatcca gcccgaccag acctccagcg
     1081 agatcagcac cagccggacg ccgaagcccc ggaacacctc gcgggccagc ggcaccaccc
     1141 ggaccagctc ggccggctcc gtctggaagg cgcccggccc gcccgcgacc atcgacgtga
     1201 ccgcctcctg ggccacccgg ggatacccgg ccgcgaccgg ctcggccggg ccggagtggc
     1261 cggccaggag ctgcaccacc tgctggcgga cccggtccag gtcggcgccc agcttgacca
     1321 gcacctgggc ggccacgccc tcgccctcgc ggatgaggcc gagcaggatg tgctcggtgc
     1381 cgatgtagtt gtggccgagc gccatggcct cgcgcagcga cagctccagg accttcttgg
     1441 cccggggcgt gaacgggatg tgggccgccg gttcctcgtc gccgcggccg atgacctcga
     1501 taacctccgt cctggccgcc tccagcgaga tgccgagctc ggtcagggcg atggccgcga
     1561 cccctttctt ctcgttgatc agcccgagca tgatgtgctc ggtgccgatg tggctgtggt
     1621 tgaggagccg ggcctcctcc tgggcgagca cgaccacccg cctggcccgg tccgtgaaac
     1681 gctcgaacat cggcacccgc ctcccgtgcg cgccgctccc gggaagccta cgccctggcg
     1741 gtcagcgcct gcggtcggcg tgacgctcga cggccaggtc gaccaggcgg gcgaccaggt
     1801 cggggtaggc gacaccggag gcggcccaga gcatggggaa catgctcttg ggcgtgaacc
     1861 cggggatggt gttgacctcg ttgagcagga cccggccggt cgcctcctcg tagaagaagt
     1921 cgacccgggc gaagccctcg cagtccagcg cctggaacgc gcgcagcgac agctcgcgca
     1981 cccgggcccg gacggcgtcg gggacctggg cggggatctc cagcttcagc gactcgtcca
     2041 ggtacttggc ctcgaagtcg tagaactcgt ggccggggat gacctcgccg accacgctgg
     2101 cctcgggggc gtcgttgccc agcaccccgc actccagctc gcgggcggcg acgccctgct
     2161 cgaccagggc cttggggtcg tgggcgaagg cggcggccag gcccgcggcc aggccgtccg
     2221 gggtcttgac cttggagatg ccgatcgagg agccgagccg ggccggcttg gtgaacaccg
     2281 ggtaggccag gcgcccggcc acctcggccg ccaccctgcc cgggtcggcc cgccagtccc
     2341 gctcgggcac gaccaggtac ggcgcctggg gcagcccggc ctgggccagc accaccttgg
     2401 ccatggcctt gtccatggcc agggccgagg cgagcacccc gctgcccaca tacggcaggt
     2461 cggccagctc gaacaggccc tgcacggtgc cgtcctcgcc gaagggcccg tgcaggatgg
     2521 ggaagacgac gtcgacgccg ccgacgccct gctcgggacg gacggccagc tccttcccgg
     2581 ccgcctcga
//
LOCUS       DASSWR010000383         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_495045,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000383 DASSWR010000000
VERSION     DASSWR010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495045"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFO47_06345"
     CDS             complement(<1..269)
                     /locus_tag="VFO47_06345"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET8788349.1"
                     /translation="MKKLINSVDDFVRESLEGMQKAHGDLIDVNYDPFVVFRTDAPVQ
                     GKVGVISGGGSGHEPMHGGFVGKGMLDAACPGEVFTSPTPDQMEWA"
     gene            complement(343..1857)
                     /gene="glpK"
                     /locus_tag="VFO47_06350"
     CDS             complement(343..1857)
                     /gene="glpK"
                     /locus_tag="VFO47_06350"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET8788350.1"
                     /translation="MAKEYVLAIDQGTTSTRAMLFQHNGTSAGAAQEEHEQIYPQAGW
                     VEHDPKEIWEKTQQVTSQVLRDNNLTAENIAAVGITNQRETTVVWDKNTGEPVYNAIV
                     WQDTRTQPIVSQWKDEGYEDEIKPKTGLVIATYFSGTKIAWILENVEGARERAEAGDL
                     LFGNTDTWLIWNLTGGVNGGHHITDYTNASRTMLFNLENTDWDDGILEKLNIPKAMLP
                     ELRPSSDRETYGSTVGDGALGGEVPIAGDAGDQQAALFGQACFDVGQAKNTYGTGNFL
                     LQNTGQQVVPSKSGLLTTGFYALEKGNSFYALEGSIAITGAAVQWLRDNLKMIANAAE
                     TEEIAQSVEDSGGAIFVPAFSGLFAPYWDERARGVIVGLTRYVNRAHLVRATLESECY
                     QSRDVVEAVEQDSGKRLEDLRVDGGSVKNNFLMQLQADILGIPVIRPQVQETTALGAA
                     YLAGLATGYWSSLDEMRENWQVDRTFEPQWSEDQRETGYAQWKKAIERTRGWVD"
     gene            complement(1949..>2532)
                     /locus_tag="VFO47_06355"
     CDS             complement(1949..>2532)
                     /locus_tag="VFO47_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HET8788351.1"
                     /translation="AKVGPYILAQVAGAFVAALLIRWNFWEWFNQVDPGKTFATQGIY
                     STSPGPGLSVLGGLRSEIIGTAVLVMLVLAITDARNTAPGANMAPFIIGLAVVVIGMS
                     LGALSGYAINPARDFGPRLASWVTGWSTAMKAPSGDLYFWVPIIGPFIGGAIGAYIYD
                     FFVGNSFPIAGEVGETVEDPDTGRPVDQRPEAT"
BASE COUNT          343 a          863 c          887 g          439 t
ORIGIN      
        1 gcccactcca tctggtcggg cgtcggcgag gtgaacacct cgcccgggca ggccgcgtcc
       61 agcatcccct tgcccacgaa cccgccgtgc atcggctcgt gccccgaccc gcccccggag
      121 atgaccccga ccttgccctg cacgggagcg tcggtccgga agacgacgaa cgggtcgtag
      181 ttgacgtcga tgaggtcgcc gtgggccttc tgcatgccct cgagcgactc cctgacgaag
      241 tcgtccaccg agttgatcag tttcttcacg ccggcctcca cggttgctcg atgggatggc
      301 ccggcgggga cagcccccgc cggacctacc ccggcaaggg atctagtcga cccacccccg
      361 ggtccgctcg atggccttct tccactgggc gtagccggtc tcgcgctggt cctccgacca
      421 ctgcggctcg aaggtccggt ccacctgcca gttctcgcgc atctcgtcca gcgaggacca
      481 gtagccggtg gccagcccgg ccaggtaggc cgcgcccagc gcggtcgtct cctggacctg
      541 gggccggatc accgggatcc cgaggatgtc ggcctggagc tgcatgagga agttgttctt
      601 gaccgagccg ccgtcgaccc gcaggtcctc caggcgcttg ccggagtcct gctccaccgc
      661 ctcgaccacg tcgcggctct ggtagcactc ggactcgagc gtcgcccgga ccaggtgggc
      721 ccggttcacg tagcgggtca ggccgacgat cacgccgcgg gcgcgctcgt cccagtaggg
      781 ggcgaacagc cccgagaacg cgggcacgaa gatcgctccg cccgagtcct cgaccgactg
      841 ggcgatctcc tcggtctcgg ccgcgttggc gatcatcttc aggttgtcgc gcagccactg
      901 gacggccgcg ccggtgatgg cgatcgagcc ctccagggcg tagaagctgt tgcccttctc
      961 gagcgcgtag aagccggtgg tcagcagccc cgacttggac ggcacgacct gctggccggt
     1021 gttctggagc aggaagttgc cggtgccgta ggtgttcttg gcctggccga cgtcgaagca
     1081 ggcctggccg aacagggccg cctgctggtc gccggcgtca ccggcgatcg ggacctcgcc
     1141 ccccagggcg ccgtcgccga cggtgctgcc gtaggtctcg cggtccgagg acggccgcag
     1201 ctcgggcagc atcgccttgg ggatgttcag cttctcgagg atgccgtcgt cccagtcggt
     1261 gttctccagg ttgaacagca tggtgcggga ggcgttggtg tagtcggtga tgtggtggcc
     1321 gccgttgacc ccgccggtca ggttccagat cagccaggtg tcggtgttgc cgaacagcag
     1381 gtcgcccgcc tcggcccgct ccctggcccc ctccacgttc tccaggatcc aggcgatctt
     1441 ggtgccggag aagtaggtgg cgatgaccag gcccgtcttg ggcttgatct cgtcctcgta
     1501 gccctcgtcc ttccactggg acacgatcgg ctgggtccgg gtgtcctgcc agacgatggc
     1561 gttgtagacc ggctcgccgg tgttcttgtc ccagacgacg gtggtctcgc gctggttggt
     1621 gatcccgacc gcggcgatgt tctcggcggt caggttgttg tcccgcagga cctgggaggt
     1681 cacctgctgg gtcttctccc agatctcctt ggggtcgtgc tcgacccagc cggcctgggg
     1741 gtagatctgc tcgtgctcct cctgggcggc gccggccgag gtgccgttgt gctggaagag
     1801 catggcccgg gtcgaggtcg ttccctggtc gatcgcgagg acgtactcct tcgccatcct
     1861 tggcctcctg tcgcgtacgg ggtgggggag tcggtcgggc ggggacggcg ccgcggtcgg
     1921 cgccgccccc gtttcacccg ttgggcggct aggttgcttc ggggcgctgg tccaccggac
     1981 ggccggtgtc cgggtcctcg accgtctcgc cgacctcgcc ggcgatgggg aacgagttgc
     2041 cgacgaagaa gtcgtagatg taggcgccga tggcgccgcc gatgaacggt ccgatgatcg
     2101 gcacccagaa gtagaggtcg ccgctgggcg ccttcatggc ggtcgaccag ccggtgaccc
     2161 aggaggccag ccgcggcccg aagtcacggg ccgggttgat ggcgtacccc gacagggcgc
     2221 ccaggctcat gccgatcacg accaccgcca ggccgatgat gaacggggcc atgttcgccc
     2281 ccggagcggt gttgcgggcg tcggtgatgg ccaggaccag catcaccagg acggccgtgc
     2341 cgatgatctc gctgcggagc ccgccaagga ccgacaggcc cggccccggg gaggtcgagt
     2401 agatgccctg ggtggcgaag gtcttgcccg ggtcgacctg gttgaaccac tcccagaagt
     2461 tccaccggat cagcagggcg gccacgaacg ccccggcgac ctgggccagg atgtacggac
     2521 cgaccttggc cc
//
LOCUS       DASSWR010000384         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_890904,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000384 DASSWR010000000
VERSION     DASSWR010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890904"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFO47_06360"
     CDS             <1..597
                     /locus_tag="VFO47_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788352.1"
                     /translation="GTTVYGGQAHIILKTISFVFVLPALAESRDLLRSFVRFPHPPAI
                     PLPSWTATALVVAGLVCVPLLYLFPDQTYPLVWLAPLAVLDGIAELRGRPSIIGLVRR
                     GRAGPVLLLAVAGLGTGILWELWNFGALPFWQYRIPYVGFFPMFEMPILGYLGYLPFI
                     LAADAFWRLFTGGAGGLAEGPVTDLGREGSGRPHPVTN"
     gene            643..1524
                     /locus_tag="VFO47_06365"
     CDS             643..1524
                     /locus_tag="VFO47_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET8788353.1"
                     /translation="METWVVVLIVIAVIALIIWGVISMVHVLQEYERGVVFRLGRVRG
                     RPKGPGLIILLPFGIDRMRKVTIQTVAMNVPPQDVITKDNVTMRVDAVIYAKVVDPVP
                     AVVRVQNYLFAVSQTAQTNLRASLGKYDLETLLSEREAINRELHGIIARVTHDWGVEV
                     LSVEVKDVDLPDDLRRAMAREAEAGREAKALLISADAELRASRVLAEAAVILSKNPQA
                     MQIRFLQTVTAVATEQNSTLVMPIPIELLGMFGGRGDQGNGLPAAPPAVSPAPPSQSP
                     PGPEEGRQPGPGNGRPG"
     gene            1723..2022
                     /locus_tag="VFO47_06370"
     CDS             1723..2022
                     /locus_tag="VFO47_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET8788354.1"
                     /translation="MATSSGAATGGDLSSTDLQIGWQYRAACKGPVSNLFFAPNHLER
                     KEERLARESAAKAICRTCPVLVECREYALLVREPHGIRGGLNEHERRQLLARRAG"
BASE COUNT          294 a          755 c          712 g          314 t
ORIGIN      
        1 ggcaccaccg tctacggcgg gcaggcccac atcatcctca agaccatctc gttcgtgttc
       61 gtgctgccgg cgctggccga gtcgcgcgac ctgctgcgct ccttcgtgcg gttcccgcac
      121 ccgccggcca tcccgctgcc gtcctggacc gccaccgccc tggtcgtcgc cggcctggtc
      181 tgcgtgcccc tgctgtatct gttccccgac cagacctacc cgctggtctg gctggccccc
      241 ctggccgtcc ttgacgggat cgccgagctg cgcggccggc cgagcatcat cggcctcgtc
      301 cgccggggcc gggccgggcc ggtgctgctg ctggccgtgg ccggtctcgg caccggcatc
      361 ctgtgggagc tgtggaactt cggcgccctg ccgttctggc agtaccgcat cccgtacgtc
      421 ggcttcttcc cgatgttcga gatgccgatc ctcggctacc tcggctacct gccgttcatc
      481 ctggccgccg acgccttctg gcgcctgttc accgggggcg ccggcggcct cgccgagggt
      541 ccggtcaccg acctcggccg cgaggggtcc ggtcgtccac atcctgtgac aaactaagcg
      601 cagcgctccg gaaccggacg acctggacgg gagggacagg ccatggagac ctgggtggtc
      661 gtcctgatcg tcatcgccgt gatcgccctg atcatctggg gcgtcatctc catggtccac
      721 gtgctgcagg agtacgagcg cggggtggtg ttccggctgg gccgcgtccg gggcaggccc
      781 aaggggcccg gcctgatcat cctgctgccg ttcggcatcg accggatgcg caaggtcacc
      841 atccagacgg tggccatgaa cgtgccgccc caggacgtga tcaccaagga caacgtcacc
      901 atgcgggtcg acgccgtgat ctacgccaag gtggtggacc cggtccccgc ggtcgtgagg
      961 gtgcagaact acctgttcgc ggtcagccag accgcccaga cgaacctccg cgccagcctc
     1021 ggcaagtacg acctggagac gttgctcagc gagcgcgagg cgatcaaccg ggagctgcac
     1081 gggatcatcg cccgggtcac ccacgactgg ggggtcgagg tcctctcggt cgaggtaaag
     1141 gacgtggacc tgcccgacga cctgcgccgg gccatggccc gcgaggccga ggccgggcgc
     1201 gaagccaagg ccctgctgat ctcggccgac gccgagctgc gggcctcgcg ggtgctggcc
     1261 gaggcggccg tgatcctgag caagaaccct caggccatgc agatccgctt cctccagacg
     1321 gtcaccgccg tggccaccga gcagaactcg accctggtca tgccgatccc gatcgagctg
     1381 ctggggatgt tcgggggacg gggcgaccag ggcaacggcc tcccggccgc gcccccggcg
     1441 gtgtcgccgg cgcccccgtc ccagagcccg ccgggaccgg aggaggggcg ccagccgggg
     1501 ccgggcaacg gccgccccgg ctagtcccct tttacgccca gttgaaggcc gtcgtgcgat
     1561 acctgggccg caacaaaagc gttactgcct ggagattgag gccgcaggtg ccgtgggtta
     1621 cgctgcgttc cggagccgct cgaccagacc gcgagcggtg tgcccagctc acgacggccc
     1681 gagggtgacg cgagagcata ggggcgagga aggaacatcg gcatggccac ctcatcgggc
     1741 gccgccaccg gcggcgacct gtcgtcgacg gatctccaga tcggctggca gtaccgtgcc
     1801 gcctgcaagg gcccggtctc caacctgttc ttcgcgccca accacctgga gcgcaaggag
     1861 gagcggctgg cccgcgagtc cgccgccaag gcgatctgcc gtacctgccc ggtcctggtc
     1921 gagtgccgcg agtacgccct gctggtgcgc gagccccacg gcatccgggg cgggctcaac
     1981 gagcacgagc gccgccagct cctggcccgc cgcgccggct gaccggtagc ctccctggcc
     2041 tgagccgacc cgccagtcaa gggagtgccg atgac
//
LOCUS       DASSWR010000385         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_523653,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000385 DASSWR010000000
VERSION     DASSWR010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523653"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VFO47_06375"
     CDS             <1..149
                     /locus_tag="VFO47_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET8788355.1"
                     /translation="YYLNVMAGPADERAWRFCDDPAHAWIRGTWDTQELDPRLPMGGA
                     GGSA"
     gene            146..2008
                     /gene="iolD"
                     /locus_tag="VFO47_06380"
     CDS             146..2008
                     /gene="iolD"
                     /locus_tag="VFO47_06380"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET8788356.1"
                     /translation="MSGTRRLTMAQALVEFLANQYSERDGREQRLFAGCFGIFGHGNV
                     AGIGQALRQYADVLPYHMARNEQSMVHIASGYAKHRNRLATFACTSSIGPGATNMVTG
                     AATATVNRLPVLLLPGDLFASRVAPPVLQQQESAGGPDVSANDAFRPVSRFWDRINRA
                     DQLPGSLLEAMRVLTDPAETGAVTLALPQDVQTEAFDYPEELFARRVWHVARPVPEPA
                     ALARAVEVLRGARRPLLVAGGGVIYAEATEALRALVEATGVPVAETYAGKGSMPYDHP
                     SALGAIGVTGTDGANAMAREADVVLGIGTRWADFATASHSIFTNPEVRFVNVNVASVD
                     AFKHAAIPLVADAREALEALAGALAGWRADDDWRETAARLNRDWDDQVERVYRAGHGP
                     LPSQGEVIGAVNDAAEPRDVVVCAAGSMPADLHKLWRTRDPKQFHLEYGNSCMGYEIP
                     GAIGIRMADPEREVFAMVGDAGYLMSPGELVTAVAERVKIIVVIVQNHGYASIGSLSE
                     ASGNGRFGTRFRRREADGTDGDLLPVDLAANAASLGAKVLTPRTAGELADALKEAREA
                     DGPVAVHVETDPAEYVTIGGAWWDVAVAEVSTSEEVQRARADYEASKGDQRHYL"
BASE COUNT          283 a          777 c          710 g          246 t
ORIGIN      
        1 tctactacct gaacgtgatg gccggacccg ccgacgagcg ggcctggcgc ttctgcgacg
       61 acccggccca cgcctggatc cgcgggacct gggacaccca ggagctcgac ccccgcctgc
      121 ccatgggcgg agcaggaggc agcgcatgag cggcacgcgc cggctgacca tggcccaggc
      181 cctggtcgag ttcctggcca accagtacag cgagcgggac gggcgggagc agcggctgtt
      241 cgccggctgc ttcggcatct tcgggcacgg caacgtggcc gggatcggcc aggccctgcg
      301 ccagtacgcc gacgtgctcc cctaccacat ggcccgcaac gagcagagca tggtccacat
      361 cgcctccggc tacgccaagc accgcaaccg gctggccacc ttcgcctgca cctcgtcgat
      421 cgggccgggc gcgaccaaca tggtcaccgg ggcggccacg gccaccgtca accgcctgcc
      481 cgtgctgctg ctcccgggcg acctgttcgc ctcccgggtg gccccgcccg tgctccagca
      541 gcaggagtcg gccggcggcc cggacgtcag cgccaacgac gccttccggc ccgtttcccg
      601 cttctgggac cggatcaacc gggccgacca gctccccggg tcgctgctgg aggccatgcg
      661 ggtcctgacc gacccggccg agaccggcgc cgtcaccctg gccctgcccc aggacgtcca
      721 gaccgaggcc ttcgactacc ccgaggagct gttcgcccgc cgcgtctggc acgtggcccg
      781 gcccgtgccc gagccggccg ccctggcccg cgccgtggag gtgctgcgcg gcgcccgccg
      841 gccgctgctg gtggccgggg gcggggtcat ctacgccgag gccaccgagg ccctgcgggc
      901 cctggtcgag gcgaccggcg tcccggtggc cgagacctac gccggcaagg gctcgatgcc
      961 ctatgaccac ccgtcggcct tgggggcgat cggcgtcacc gggaccgacg gggccaacgc
     1021 catggcccgc gaggccgacg tggtgctcgg gatcggcacc cgctgggccg acttcgccac
     1081 cgcctcgcac agcatcttca ccaaccccga ggtccggttc gtcaacgtca acgtggcttc
     1141 ggtggacgcc ttcaagcacg ccgccatccc cctggtcgcc gacgcccgcg aggccctgga
     1201 ggcgctggcc ggggccctgg ccggctggcg ggccgacgac gactggcgcg agacggcggc
     1261 ccggctcaac cgcgactggg acgaccaggt ggagcgcgtc taccgggccg ggcacgggcc
     1321 gctgcccagc cagggtgagg tcatcggggc cgtcaacgac gccgccgagc cccgcgacgt
     1381 ggtcgtctgc gccgccggca gcatgccggc cgacctgcac aagctgtggc ggacccgcga
     1441 ccccaagcag ttccacctcg agtacggcaa ctcctgcatg ggctacgaga tccccggcgc
     1501 catcgggatc cgcatggccg accccgagcg tgaggtgttc gccatggtcg gcgacgccgg
     1561 ctacctgatg agcccgggcg agctggtcac ggccgtggcc gagcgggtca agatcatcgt
     1621 ggtcatcgtc cagaaccacg gctacgcctc gatcgggtcg ctgtcggagg cgtcgggcaa
     1681 cggccgcttc gggacccggt tccgccgccg cgaggccgac gggacagacg gcgacctgct
     1741 cccggtcgac ctggccgcca acgcggccag cctcggggcc aaggtcctga ccccgcggac
     1801 cgccggcgag ctcgccgacg ccctcaagga ggcccgcgag gccgacgggc cggtggccgt
     1861 ccacgtggag accgacccgg ccgagtacgt gaccatcggc ggcgcctggt gggacgttgc
     1921 cgtggccgag gtctccacct ccgaggaggt ccagcgggcc agggccgact acgaggccag
     1981 caaaggagac cagcggcact acctgtagcc caacct
//
LOCUS       DASSWR010000386         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1371224, whole genome shotgun sequence.
ACCESSION   DASSWR010000386 DASSWR010000000
VERSION     DASSWR010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371224"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VFO47_06385"
     CDS             complement(<1..1418)
                     /locus_tag="VFO47_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET8788357.1"
                     /translation="METLELLIGLLAAVAVVVRLAGRTAIPEPVLLVLAGLAVALIPG
                     LPQVELDPELILALFLPPLLYWAALHTDLEELRGNLRPIALLAVGLVLVTTAAVAVLG
                     HTVLGLPLAVAVVLGAIVSPPDPVAATAVCGRLGLPRRMAAILEGEGLLNDATALVLY
                     RMAVAAAVSGAFSLVDAGIELGVSAVGGTAVGLAVGWAGSRILRRVSEAPVENTVKLL
                     LPYVAWLAAERVHVSGVLAVLACGVLMTRHWSAISSGARLQARQLWDWLVFVLEGLSF
                     VLVGVQLRTVVEGIEGRSLADLALAALAVNLVVIVVRLALVFPASWLPRLSARVRERD
                     PYPGWRRLTVVGWAGMRGVVTLALALAIPTEVEGGGPFPDRNLVVFLAFSVIVVTLVG
                     EGLTLPLVIRRLGVGAEDDGAAGDGRRALARLSEVALDHLDAVDPEAGGVPGELVERL
                     RERYRARLAHLDQQQREDSDGRP"
     gene            1510..>2300
                     /locus_tag="VFO47_06390"
     CDS             1510..>2300
                     /locus_tag="VFO47_06390"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET8788358.1"
                     /translation="MDDEHHPHEREWRGTLPEVDEETFLRVLTEAIAVAGGTGLPHAF
                     MGGIASTALGRPRWTHDIDLCVRRDDARTMLRAFADAGYDTEETDQTWLYKATKDGVL
                     VDVIFESTGGIVLDDEMLSRVRQATFEDLQLNVLGPEDLLVIKAIVHREHRQRHWFDA
                     LALVGRGELDWPYLLRRAAPGPRRVASLLLYAQTDGLRVPDWVIEALLEQAREQEAGP
                     DVPEEYLVAHASEALAADPRTAELELDIAVDDREVVVTGTVATPER"
BASE COUNT          349 a          903 c          825 g          223 t
ORIGIN      
        1 ggccgcccgt cgctgtcctc gcgctgctgc tggtccaggt gggccagccg ggcccggtag
       61 cgctcgcgga gccgctcgac cagctcgccc gggaccccgc cggcctccgg gtcgaccgcg
      121 tccaggtggt cgagggcaac ctcggacagc cgggccaggg cccggcggcc gtcgccggcc
      181 gccccgtcgt cctcggcccc cacccccagc cgccggatca ccaggggcag ggtcaggccc
      241 tcgccgacca gggtgaccac gatcaccgag aaggccagga acacgaccag gttgcggtcc
      301 gggaatggcc cgcccccctc gacctcggtc gggatggcca gggcgagggc gagggtcacc
      361 accccgcgca tgcccgccca gccgaccacc gtgagccgcc gccagcccgg gtaggggtcg
      421 cgctcgcgca cccgggccga cagccgcggc agccagctgg ccgggaacac cagggccagg
      481 cgcacgacga tgacgaccag gttgacggcc agggcggcca gggccaggtc ggccagggag
      541 cggccctcga tcccctcgac cacggtgcgc agctgcaccc cgacgagcac gaacgacagg
      601 ccctcgagga cgaacaccag ccagtcccag agctggcggg cctggagccg ggcccccgag
      661 gagatggcgc tccagtggcg cgtcatcagc accccgcagg cgagcacggc cagcaccccc
      721 gagacatgga cccgctcggc ggccagccag gccacgtacg gcagcagcag cttgacggtg
      781 ttctcgaccg gcgcctcgga cacccggcgc aggatgcggc tgccggccca gccgacggcc
      841 agccccaccg ccgtcccccc gacggccgac acgcccagct cgatcccggc gtcgaccagc
      901 gagaaggcgc cggagacggc cgcggccacc gccatccggt acaggaccag ggcggtggcg
      961 tcgttgagca gcccctcgcc ctccaggatg gcggccatcc gcctgggcag tcccaggcgg
     1021 ccgcagacgg cggtcgcggc caccgggtcg ggcggcgaca cgatcgcccc cagcaccacc
     1081 gccaccgcca acggcagccc gagcaccgtg tggcccagca ccgccaccgc ggccgtggtc
     1141 accagcacca gccccacggc cagcagggcg atcggcctga gattgccgcg cagctcctcc
     1201 aggtcggtgt ggagcgccgc ccagtacagc agcggcggca ggaacagggc caggatcagc
     1261 tccgggtcga gctcgacctg gggcagcccc gggatcaggg cgacggccag gccggccagc
     1321 accagcagca ccggctcggg gatggcggtg cggccggcca gcctgaccac caccgccacg
     1381 gcggcgagca ggccgatcag cagctcaagg gtctccatga cggggcaacg gtggcacgcg
     1441 ccggtcgcgc ccgccacccg gcgcccaccg gcgcgtttcg tcgcctgccg ggcgtggaac
     1501 agccaggaca tggacgacga gcaccacccc cacgagcgcg agtggcgggg cacccttccc
     1561 gaggtcgacg aggagacctt cctgcgggtg ctcaccgagg ccatcgcggt cgccggcggc
     1621 accggcctgc cgcacgcctt catgggcggg atcgcctcga ccgcgctggg ccggccccgc
     1681 tggacccacg acatcgacct gtgcgtgcgc cgcgacgacg cccggacgat gctgcgcgcc
     1741 ttcgccgacg ccggctacga caccgaggag accgaccaga cctggctcta caaggcgacc
     1801 aaggacggcg tcctggtcga cgtgatcttc gagagcaccg gcgggatcgt gctcgacgac
     1861 gagatgctgt cgcgggtccg ccaggccacc ttcgaggacc tccagctcaa cgtcctcggc
     1921 cccgaggacc tgctggtgat caaggcgatc gtccaccgcg agcaccgcca gcggcactgg
     1981 ttcgacgccc tcgccctggt cgggaggggc gagctcgact ggccctacct gctgcgccgg
     2041 gccgccccgg ggccgcgccg ggtggccagc ctgctgctgt acgcccagac cgacgggctg
     2101 cgggtgcccg actgggtgat cgaggcgctg ctggagcagg cccgcgagca ggaggccggg
     2161 cccgacgtgc ccgaggagta cctggtcgcg cacgccagcg aggccctggc cgccgacccc
     2221 cgcacggccg agctggagct ggacatcgcc gtcgacgacc gcgaggtggt ggtgaccggg
     2281 acggtggcca cgcccgagcg
//
LOCUS       DASSWR010000387         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_552332,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000387 DASSWR010000000
VERSION     DASSWR010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552332"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1741
                     /locus_tag="VFO47_06395"
     CDS             134..1741
                     /locus_tag="VFO47_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoF family protein"
                     /protein_id="HET8788359.1"
                     /translation="MGSSRPWARASAHVCQAASCLSASSDQIFDALAEQVTEAGLTDV
                     AVKRVGCLGLCAAGPLVEVPETGRLFDRVRPDDLGGLVEELQAVSPGTTPAPSPPFFA
                     RQVRVATENSGRVDPEDLDDYRARGGYRALETALHSMTPAEVIDQVVRSGLRGRGGAG
                     YPTGLKWTTVAKAAGGQKYVICNADEGDPGAFMDRSVLESDPHRVLEGMAIAGYAVGA
                     SRGYVYCRAEYPLAVARLKTAIRLAGRAGLLGADIADTPFAFQVELRLGAGAFVCGEE
                     TALIASVQGGRGMPRPRPPYPAVSGLWRSPTLINNVETFANIAPIVRNGGDWYAGIGT
                     ETSKGTKVFALAGRVVNTGLVEVPMGITLREIVYDIGGGILDGRPFKAVQTGGPSGGC
                     IPAQYLDMPVDYESLKEVGSIMGSGGMIVMDDTACMVDVARYFMDFCREESCGKCIPC
                     RVGTTQLHTLLQRICEGDATLDDLEQLERLSDMVKQTSLCGLGQSAPNPVFSTLRYFR
                     EEYLAHVQDRTCPAGVCPVANREEVLG"
     gene            1738..2448
                     /gene="hoxU"
                     /locus_tag="VFO47_06400"
     CDS             1738..2448
                     /gene="hoxU"
                     /locus_tag="VFO47_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HET8788360.1"
                     /translation="MSVYTLRIDGTDVAGAAGQSILDVAAENSIEIPTLCHLEGLSDV
                     GACRLCVVEVGASGKLLPACVTAVEEGMEVTAHSERLAAYRRTIVEMLFVERNHICSV
                     CVANNHCELQDLAATLGLTHFELPAVNPRVGIDASHPLFAIDHNRCVMCTRCVRVCDE
                     IEGAHTWDVMGRGIDARVVTDLGTPWGESPTCTSCGKCVQVCPTGALFEKGRSVAEGA
                     KTRRPFLPYLKRRGEALE"
     gene            2445..>2814
                     /locus_tag="VFO47_06405"
     CDS             2445..>2814
                     /locus_tag="VFO47_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HET8788361.1"
                     /translation="MSRPRLATVWLDGCSGCHMSFLDMDERLLELGQRADLVYSPLVD
                     TKDYPEGVDVCLVEGAVSSDEDLAKIKAIRDRTRILVSFGDCAVTSNVPGMRNPIGPL
                     PLLERAYLENATDNPQLPSQV"
BASE COUNT          398 a         1014 c          988 g          414 t
ORIGIN      
        1 gaggccgggc tgaccgacgt ggccgtcaag cgggtcggct gccttggcct gtgcgccgcc
       61 ggccccctcg tcgaggtccc cgagagcggc cggctgttcg agcgggtcgg cccggacgac
      121 ctcggcggcc tggttgggga gctcacggcc gtgggcccgg gcgtcggccc atgtctgcca
      181 ggctgccagc tgcctgtccg cctcctccga ccagatcttc gacgccctgg ccgagcaggt
      241 caccgaggcc gggctgaccg atgtggccgt caagcgggtc ggctgcctgg gcctgtgtgc
      301 cgccggcccc ctggtcgagg tccccgagac cggccggctg ttcgaccgcg tccgccctga
      361 cgacctgggt ggcctggtcg aggagctgca ggccgtcagc cccgggacga ccccggcgcc
      421 gtcgccgccg ttcttcgccc gccaggtccg ggtggccacc gagaactccg ggcgggtcga
      481 ccccgaggac ctggacgact accgggcccg cggcggctac cgggcgctgg agacggcgct
      541 gcactcgatg accccggccg aggtgatcga ccaggtcgtc cgcagcggcc tgcgagggcg
      601 gggcggagcc ggctacccga ccgggctgaa gtggaccacg gtggccaagg ccgccggcgg
      661 gcagaagtac gtgatctgca acgccgacga gggggacccg ggggcgttca tggaccgcag
      721 cgtgctcgag agcgacccgc atcgggtgct ggagggcatg gccatcgccg gctacgccgt
      781 gggggccagc cgcgggtatg tctactgccg ggccgagtac ccgctggccg tggcccgcct
      841 caagacggcc atccgcctgg ccggacgggc cgggctgctg ggcgccgaca tcgccgacac
      901 tccctttgcg ttccaggtgg agctgcgtct tggcgcgggc gcgttcgtct gcggcgagga
      961 gacggccctg atcgcctcgg tccaaggtgg gcggggtatg ccccggcccc ggccgccgta
     1021 cccggccgtc tccgggctgt ggcgaagccc gacgctgatc aacaacgtcg agacgttcgc
     1081 caacatcgcc cccatcgtcc gcaacggcgg ggactggtac gccggcatcg gcacggagac
     1141 cagcaagggc accaaggtgt ttgccctggc cggccgggtc gtcaacaccg gcctggtcga
     1201 ggtccccatg gggatcaccc tgcgcgagat cgtctatgac atcggtggcg ggatcctgga
     1261 cggccgcccg ttcaaggccg tccagaccgg cggtccctcg gggggctgca tccccgcgca
     1321 gtacctggac atgccggtcg actacgagtc gctgaaggag gtcggctcga tcatgggctc
     1381 gggcggcatg atcgtgatgg acgacaccgc ctgcatggtg gatgtggccc gctacttcat
     1441 ggacttctgc cgcgaggagt cctgcggcaa gtgcatcccc tgccgggtcg gcaccaccca
     1501 gctccacacc ctgctgcaac gcatctgcga aggcgacgcc actctggacg acctggagca
     1561 gttggagcgg ctgtcggaca tggtcaagca gaccagcctg tgtggcctgg gccagagcgc
     1621 ccccaacccg gtgttctcga ccctgcgcta cttccgcgag gagtacctgg cccacgtcca
     1681 ggaccggacc tgcccggccg gggtctgccc ggtcgccaac cgggaggagg tgctaggttg
     1741 agcgtctaca ccctgcggat cgacggcacc gacgtggccg gcgccgccgg ccaatcgatc
     1801 ctggacgtgg ccgccgagaa cagcatcgag atcccgaccc tgtgccacct ggagggcctc
     1861 agcgacgtcg gcgcctgccg cctgtgcgtc gtcgaggtcg gcgcctccgg caagctcctg
     1921 ccggcctgtg tcaccgcggt cgaggagggc atggaggtga ccgcccactc cgagcggctg
     1981 gccgcctacc gccggacgat cgtcgagatg ctgttcgtgg agcgcaacca catctgctcg
     2041 gtgtgcgtcg ccaacaacca ctgcgagctc caagatctgg ccgccaccct cggcctcacc
     2101 cacttcgagc tgcccgcggt caacccgcgg gttgggatcg acgccagcca cccactgttc
     2161 gccatcgacc acaaccgctg tgtcatgtgc acccgctgcg tccgggtctg cgacgagatc
     2221 gagggcgctc acacctggga cgtcatgggc cgcggcatcg acgcccgggt ggtcaccgac
     2281 ctcggcactc cctgggggga gtcgcccacc tgcaccagct gcggcaagtg cgtccaggtc
     2341 tgcccaaccg gggcgctgtt cgagaagggc cgctcggtgg ccgagggggc caagacccgc
     2401 cggcccttcc ttccctacct caagcgccga ggggaggccc tggaatgagc aggccgcggc
     2461 tggccaccgt ctggctggac ggctgctccg gctgtcacat gtcgttcctg gacatggacg
     2521 agcggctgct ggagctgggc cagcgggccg acctggtcta cagccccctg gtcgacacca
     2581 aggactaccc cgagggcgtc gacgtctgcc tggtcgaggg cgccgtctcc agcgacgagg
     2641 acctggccaa gatcaaggcc atccgggacc gcacccggat cctggtgtcg ttcggcgact
     2701 gcgccgtcac ctccaatgtc cccggcatgc gcaacccgat cggccccctc ccgttgctgg
     2761 agcgggcgta tctggagaac gccaccgaca acccgcaact gccctcccag gttg
//
LOCUS       DASSWR010000388         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1286817, whole genome shotgun sequence.
ACCESSION   DASSWR010000388 DASSWR010000000
VERSION     DASSWR010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286817"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VFO47_06410"
     CDS             <1..1001
                     /locus_tag="VFO47_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8788362.1"
                     /translation="EWSPSYATARAAGILRGMLERGFTTVRDVAGADFGIAAAVAEGL
                     LPGPRVIFGGKALSQTAGHGDSRGPVRVVLDQLPCCPTHTVVADGVPEVRRAAREQLR
                     RGAHHVKVMLSGGVASPTDRIDSTQYSMEELRAVVEEARAAGRYVTGHAYTAAAVNRG
                     LEAGVGCIEHGNLMDGSSVELFRRTGAFYVPTLATYAALAEEGLEHGMPADQHAKVFE
                     VLDAGLGALELAHRAGLPIAYGTDLLGGMHRRQLTEFTIRSEVQRPADILRSATTVPA
                     RLVGLEGRAGVVAPGAFADLLVVDGNPLERLEVLTDPARHLRLVMAAGRVHRNDLA"
     gene            complement(998..1336)
                     /locus_tag="VFO47_06415"
     CDS             complement(998..1336)
                     /locus_tag="VFO47_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET8788363.1"
                     /translation="MDELAARVLAVVGRIPWGRVLTYGDVAALAEGDATARDVGQVLL
                     RHGEREDVPWWRVLRADGTPPPHLLDRQLALLRSEGTPLAPSGAAVDLHAARWSASGR
                     GQDAQPSLFD"
     gene            complement(1336..1920)
                     /locus_tag="VFO47_06420"
     CDS             complement(1336..1920)
                     /locus_tag="VFO47_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HET8788364.1"
                     /translation="MTPPTLRLAQDPEADALLARDPLALLMGMLLDQQFPLEWAFRAP
                     WRLAQRMERTSLDAAELAGWDPDAFVKLMAGPPALHRYPAAMAGRFQTLCRIVVDQYG
                     GDAAAIWRTAASGKELLARLRALPGFGEQKARIFVALLGKQLGVRPEGWREAAGPYGE
                     DGSRRSAADVTDADTLAEVRAFKQAAKRAAKGTG"
     gene            complement(1917..>2863)
                     /locus_tag="VFO47_06425"
     CDS             complement(1917..>2863)
                     /locus_tag="VFO47_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET8788365.1"
                     /translation="WRALRLLWFGLVWLAMESVALFAALALWVASGFGGRLSSEEYLE
                     RHYALMRWWLAVVFRVAARTFRLRIEIEEPELTPEEARARLTRPVIVLSRHAGPGDSF
                     LLVHHLLSRYGRKPRIVMKAALQFDPSLDVIINRLPNAFVPARHGAGNLVIKEIQRLA
                     AGLGPTGALVIFPEGGNFTPRRRLRAIRLLEEHRLAKEAARARAMEHLLPPKPNGALA
                     AIEAAPTADVVFVAHTGLEHVITLGDVWRALPMEQVLKARWWRVPAAEVPEGREAQVD
                     WLYDWWARIDAWIRDNRPTALPRQDRQVGAAVQIGGQR"
BASE COUNT          356 a         1047 c         1114 g          346 t
ORIGIN      
        1 aggagtggtc gccgtcgtac gccaccgcgc gggcggccgg gatcctgcgg gggatgctgg
       61 agcgggggtt cacgaccgtg cgcgacgtgg ccggggccga cttcgggatc gcggccgcgg
      121 tcgccgaggg gctgctgccc ggaccccggg tcatcttcgg gggcaaggcc ctgtcccaga
      181 ccgcgggaca cggcgactcc cgggggccgg tccgggtcgt gctcgaccag ctcccctgct
      241 gcccgaccca caccgtggtc gccgacggcg ttcccgaggt ccgccgcgcg gcccgggagc
      301 agctgcgccg cggcgcccac cacgtgaagg tgatgctgtc gggcggggtg gcctccccca
      361 ccgaccggat cgactcgacc cagtactcga tggaggagct gcgggcggtc gtggaggagg
      421 cgcgggcggc cggccgctac gtcaccgggc acgcctacac cgccgccgcc gtcaaccggg
      481 ggctggaggc gggggtcggc tgcatcgagc acggcaacct gatggacggg tcctcggtcg
      541 agctgttccg gcggaccggg gccttctacg tgcccaccct ggccacctac gccgccctgg
      601 ccgaggaggg actggagcac ggcatgcccg ccgaccagca cgccaaggtg ttcgaggtgc
      661 tcgacgccgg gctgggggcg ctggagctgg cccaccgggc cgggctgccg atcgcctacg
      721 gcaccgacct gctcggcggc atgcaccgcc gccagctcac cgagttcacc atccggtccg
      781 aggtccagcg gcccgccgac atcctccggt cggcgaccac tgtgcccgcc cgcctggtcg
      841 gcctcgaggg ccgtgccggc gtggtcgccc ccggcgcctt cgccgacctc ctggtggtcg
      901 acggcaaccc cctggagcgc ctggaggtcc tcaccgaccc cgcccgccac ctccgcctgg
      961 tcatggcggc cggccgggtc caccgcaacg acctggccta gtcgaacagg gacggctggg
     1021 cgtcctggcc gcggccggag gcggaccagc gggcggcgtg gaggtcgacg gcggcgccgg
     1081 agggggcgag gggggtgccc tcggagcgga gcagggcgag ctggcggtcg agcaggtggg
     1141 gcgggggcgt gccgtcggcg cgcagcacgc gccaccaggg gacgtcctcg cgctcgccgt
     1201 ggcggagcag gacctggccg acgtcgcggg cggtggcgtc gccttcggcc agggcggcca
     1261 cgtcgccgta ggtcaggacc cgcccccatg ggatccgccc gaccacggcc aggaccctgg
     1321 ccgcgagctc gtccactagc cggtcccctt ggcggcgcgc ttggccgcct gcttgaaggc
     1381 gcggacctcg gccagggtgt cggcgtcggt gacgtcggcg gcggagcggc gcgacccgtc
     1441 ctcgccgtag ggcccggccg cctcccgcca gccctcgggc cggaccccga gctgcttgcc
     1501 cagcagggcg acgaagatcc gggccttctg ctcgccgaag ccgggcaggg cgcgcagccg
     1561 ggccagcagc tccttgccgg aggcggcggt ccgccagatg gcggcggcgt ccccgccgta
     1621 ctggtcgacc acgatgcggc agagcgtctg gaaccgcccg gccatcgccg ccgggtagcg
     1681 gtgcagggcc ggcgggccgg ccatcagctt gacgaacgcg tccgggtccc agccggccag
     1741 ctcggccgcg tcgagcgacg tccgctccat ccgctgggcc agccgccagg gcgcccggaa
     1801 cgcccattcc agggggaact gctggtcgag cagcatcccc atgagcaggg ccagcgggtc
     1861 cctggcgagc agcgcgtcgg cctcggggtc ctgggccagc cgcagggtgg gtggtgtcac
     1921 cgttgacctc cgatctggac ggccgcgccg acctggcggt cctggcgggg cagggcggtg
     1981 gggcggttgt cacggatcca ggcgtcgatg cgcgcccacc agtcgtacag ccagtcgacc
     2041 tgggcctccc ggccctccgg gacctcggcg gcgggcacgc gccaccagcg ggccttgagg
     2101 acctgctcca tgggcagggc ccgccagacg tcgccgaggg tgatcacgtg ctccaggccg
     2161 gtgtgggcga cgaagacgac gtcggcggtg ggggcggcct cgatcgcggc cagggcaccg
     2221 ttcggcttgg gcgggagcag gtgctccatg gccctggccc tggccgcctc cttggccagg
     2281 cggtgctcct ccagcagccg gatggcccgg agccgccgac gcggggtgaa gttgccgccc
     2341 tcggggaaga tcaccagcgc cccggtgggg cccaggccgg cggcgaggcg ctggatctcc
     2401 ttgatgacca ggttgccggc gccgtgccgg gccgggacga aggcgttggg caggcggttg
     2461 atgatcacgt cgaggctggg gtcgaactgg agggccgcct tcatgacgat ccggggcttg
     2521 cgcccgtagc ggctgagcag gtgatgcacg agcaggaacg agtcgccggg gccggcgtgg
     2581 cggctgagca cgatcaccgg ccgggtcagg cgggccctgg cctcctcggg ggtgagctcc
     2641 ggctcctcga tctcgatgcg caggcggaag gtccgggccg ccacccggaa caccaccgcc
     2701 agccaccagc gcatcagcgc gtagtggcgt tccaggtact cctccgagct cagccggccg
     2761 ccgaagccgc tggccaccca gagggccagg gcggcgaaca gggccaccga ctccatggcc
     2821 agccagacca gcccgaacca gagcagccgc agggcccgcc agt
//
LOCUS       DASSWR010000389         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1314729, whole genome shotgun sequence.
ACCESSION   DASSWR010000389 DASSWR010000000
VERSION     DASSWR010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314729"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VFO47_06430"
     CDS             <1..1266
                     /locus_tag="VFO47_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET8788366.1"
                     /translation="EFWRAPGTNPATVQTEVFMLPAPHWIEKDGSFTNSGRWAQWKDQ
                     VLPPEGEARHDHWIMAELFQRVRDLYRRQGGKYPAPLLELSMPYRDPVKPELDEIAQE
                     INGRDLRTGKRLETFADLKDDGTTTAGDWIYTGSYPESGNLAKRRDGVQDPAVNDPTG
                     MGFYHNWGWSWPLNRRVLYNRASADPQGRPWDPERAGIRWDAAAGKWVGDVPDYPPDA
                     DPRDPKAPLPFIMTGEGTGRLFSSSVADGPFPEHYEPIESPVDNPLHPEVSATPVAFL
                     YDQAAGRPNRFGTRDEYPYVATSYRLTEHEHYITQQVEQLVQLQPEAFVEVPAELASE
                     KGIANGDLVRVSSKRGKLEVRAVVTGRLGPLQIDGKKVYQIGIPIHWGFVGIKTGQHW
                     LANALTPFVGDASARTPEFKAFLVNIEKL"
     gene            1266..2123
                     /gene="fdhE"
                     /locus_tag="VFO47_06435"
     CDS             1266..2123
                     /gene="fdhE"
                     /locus_tag="VFO47_06435"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HET8788367.1"
                     /translation="MTALGATATSPWTPQRRRAEALRARHPFAAEVLALYVALLDVWD
                     EAWDAAHRERPEPPALAGWAAGRVLPRVVEATAKAGPEPLAGATRGLLEAGGTSEPLA
                     AWLAGDELAPAERYLARATLRGPLQALDADAACARDPSPRGGRHCPRCGGPPQLSYRA
                     HSDDGLVSEHRHLACARCGATWRYSGSACASCGETKAARRTIYAERRESPVVGRGDPA
                     APTGPDGPMFPHVRIEGCDSCKRYLIDVDLGRDARAVPEVDELAALPLDLHAAELGLS
                     KITPNLMGF"
     gene            2125..>2260
                     /locus_tag="VFO47_06440"
     CDS             2125..>2260
                     /locus_tag="VFO47_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET8788368.1"
                     /translation="MAEIVPGQAYGFFTDTSVCIGCKACEVACKQWNMLSGHEPSFID
                     G"
BASE COUNT          345 a          854 c          786 g          275 t
ORIGIN      
        1 gagttctggc gcgcacccgg caccaacccg gcgacggtcc agaccgaggt cttcatgctg
       61 ccggcccccc actggatcga gaaggacggc tcgttcacca acagcggccg ctgggcgcag
      121 tggaaggacc aggtcctgcc cccggagggc gaggcccgcc acgaccactg gatcatggcc
      181 gagctgttcc agcgggtgcg cgacctctac cgccggcagg gtgggaagta cccggcgccg
      241 ctgctggagc tgtccatgcc gtaccgggac ccggtcaagc ccgagctgga cgagatcgct
      301 caggagatca acggccggga ccttcgcacc ggcaagcggc tggagacctt cgccgacctc
      361 aaggacgacg gcacgaccac cgccggcgac tggatctaca ccggcagcta cccggagagc
      421 ggcaacctgg ccaagcgccg cgacggcgtc caggaccccg cggtcaacga cccgacgggc
      481 atgggtttct accacaactg ggggtggagc tggccgctca accggcgcgt gctctacaac
      541 cgggcctcgg ccgaccccca gggccgcccg tgggatccgg agcgggcggg gatccgctgg
      601 gacgccgccg ccggcaagtg ggtgggcgac gtgcccgact acccgcccga cgccgaccca
      661 cgcgacccca aggccccgct gccgttcatc atgaccgggg aggggacggg gcggctgttc
      721 agcagctcgg tggcggacgg gccgttcccc gagcattacg agccgatcga gtcgccggtc
      781 gacaaccccc tgcaccccga ggtctcggcc acgcccgtgg ccttcctcta cgaccaggcg
      841 gccggccggc ccaaccggtt cggcacccgg gacgagtatc cgtacgtggc caccagctac
      901 cggctcaccg agcacgagca ctacatcacc cagcaggtcg agcagctggt ccagctgcaa
      961 ccggaggcgt tcgtcgaggt gcccgccgag ctcgccagcg agaagggcat cgccaacggc
     1021 gacctggtcc gggtttcgtc caagcggggc aagctcgagg tccgggcggt ggtgaccggg
     1081 cgcctcggcc cgctccagat cgacggcaag aaggtctacc agatcggcat ccccatccac
     1141 tggggcttcg tcggcatcaa gaccggccag cactggctcg ccaacgcgct cacgccattc
     1201 gtcggcgacg ccagcgcacg cacccccgag ttcaaggcgt tcctcgtgaa catcgagaag
     1261 ctctgatgac cgcactcggc gcgaccgcga ccagtccgtg gacgccccag cgccgacggg
     1321 ccgaggccct gcgcgcccgc caccccttcg cggcggaggt gctcgccctc tacgtcgccc
     1381 tgctcgacgt ctgggacgag gcatgggacg ccgcccaccg ggagcggccg gagcctccgg
     1441 cgctggccgg ctgggccgcc gggcgcgtgc tcccgcgggt cgtggaggcg acggcgaagg
     1501 ccggcccgga accgctggcc ggcgccaccc gtgggctcct cgaagcaggc ggcacctccg
     1561 aaccgctggc cgcctggctc gccggcgacg agctcgcgcc ggccgagcgc tacctggccc
     1621 gcgccaccct gcgggggccg ctgcaagcgc tcgacgccga cgccgcctgc gcccgggacc
     1681 cgtcgcccag gggcggccgg cactgtccgc gctgcggcgg accgccgcag ctcagctacc
     1741 gcgcccactc cgacgacggg ctggtgagcg agcaccggca cctggcctgc gcccgctgcg
     1801 gggcgacctg gaggtattcc ggcagcgcct gcgcctcgtg cggggagacc aaggccgccc
     1861 ggcgcaccat ctacgccgag cggcgcgagt cgcccgtggt ggggcgcggc gatcccgcgg
     1921 cacccacggg gcccgacggc ccgatgttcc cgcacgtccg catcgagggc tgtgacagct
     1981 gcaagcgcta cctgatcgac gtcgacctgg gccgcgacgc ccgagccgtg cccgaggtgg
     2041 acgagctggc ggcgctgccg ctggacctcc acgcagccga gctgggccta tccaagatca
     2101 cacccaacct gatggggttc tgagatggcc gagatcgtgc ctggccaggc ctacgggttc
     2161 ttcaccgaca ccagcgtctg catcggctgc aaggcctgcg aggtggcctg caagcagtgg
     2221 aacatgctct cgggccatga gccgtcgttc atcgacggct
//
LOCUS       DASSWR010000390         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_749292,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000390 DASSWR010000000
VERSION     DASSWR010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749292"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1337
                     /gene="aroC"
                     /locus_tag="VFO47_06445"
     CDS             126..1337
                     /gene="aroC"
                     /locus_tag="VFO47_06445"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET8788369.1"
                     /translation="MLRWLTAGESHGPELLAIVEGLPAGIPVTLDEIRLDLARRKLGY
                     GRGARMKFEQDELNISGGVRHGLTLGGPVALRIGNTEWPKWAEVMSPDPVEDPEAAAT
                     GKGVALTRPRPGHADLTGMQKYGFEEARPVLERASARETAARVAAGAAAKALLGQVGV
                     SVLSHVVRLGPAATRSRAVPTLADLEEIDASPVRCLDGEAEGRMAAAVDAAKAAGDTL
                     GGVFEVVAHGLPPGLGSYVHWDRKLDARLAGALMSIQAIKGVEVGDGFAGAARPGSRA
                     HDAIVPGARPGTLRRRTGRAGGIEGGMSTGEVLRVRAAMKPLSTLVRALDTVDVATGA
                     RAKAVVQRSDVTAVPAAGVVGEAVVALVLADALLEKTGGDSLAEVRRNLDAYLSGLDP
                     QDPAGPQGAGG"
     gene            complement(1280..1585)
                     /locus_tag="VFO47_06450"
     CDS             complement(1280..1585)
                     /locus_tag="VFO47_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788370.1"
                     /translation="MRRFLLLVFFSCTALSVAFSLWPRQLGGVLALFGQDAVVNRGVA
                     ATVLVVLTMLLLALRVRLWRSERDLARIRRGLAAPPAVPYPPAPWGPAGSWGSRPLR"
     gene            1733..2278
                     /locus_tag="VFO47_06455"
     CDS             1733..2278
                     /locus_tag="VFO47_06455"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET8788371.1"
                     /translation="MRPILPTIVVIGFMGVGKTSVGRLIARALGREFVDTDLMIERAA
                     RMTIAELFQREGELSFRRREMAAIDRAISVPGRVIAVGGGAVLSAENRTALKQAGLLV
                     YLRATPETLAGRLTGVTDRPLLGDTADRAGRIRDLLVARGPIYETAGDVAIDTDRLNV
                     EQVAVEVMEWYRRRVGAMAGR"
BASE COUNT          322 a          930 c          898 g          274 t
ORIGIN      
        1 gcacagccca ccactgcaca gcccaccact gcacagccca ccactgcaca gcccacaccc
       61 gcacagccca cccccggcct gaccggcgcg gcttcggccg gccaccctgt ggaaggatag
      121 aacccatgct tcgttggctc accgccggag agtcccacgg ccccgaactc ctcgctattg
      181 tcgagggttt gcccgctggg atcccggtca ccctagatga gatccgcctc gaccttgcgc
      241 gccgcaaact cggctacggc cgcggcgccc gcatgaagtt cgagcaggac gaactgaaca
      301 tctccggcgg cgtacgccac ggcctcaccc tcgggggccc cgtcgctctg cgcatcggca
      361 acaccgaatg gcccaagtgg gccgaggtga tgagcccgga cccggtcgag gacccggagg
      421 cggccgccac cggcaagggg gtggccctga cccggccccg gcccggccac gccgacctga
      481 ccggcatgca gaagtacggc ttcgaggagg cccggccggt gctggagcgg gccagcgccc
      541 gcgagacggc ggcccgggtg gccgccgggg cggccgccaa ggccctgctc ggccaggtcg
      601 gggtgtcggt gctcagccac gtggtccggc tcgggccggc cgccacccgg tcacgggccg
      661 tgcccaccct ggccgacctg gaggagatcg acgcctcgcc cgtccgctgc ctggacgggg
      721 aggccgaggg ccggatggcg gccgcggtcg acgcggccaa ggcggccggc gacaccctcg
      781 gcggggtgtt cgaggtggtc gcccacgggc tgccgcccgg cctcgggtcc tacgtccact
      841 gggaccgcaa gctggatgcc cgcctggccg gggccctgat gagcatccag gccatcaagg
      901 gggtcgaggt cggcgacggc ttcgccggcg ccgcccggcc cgggtcgagg gcccacgacg
      961 ccatcgtccc cggggcccgc cccggcaccc tgcggcggcg gacggggcgg gccgggggga
     1021 tcgagggcgg catgtcgacc ggcgaggtgc tgcgggtccg ggcggccatg aagccgctgt
     1081 cgaccctcgt ccgggccctg gacacggtgg acgtggccac cggggccagg gccaaggcgg
     1141 tggtgcagcg ctcggacgtg accgccgtcc cggcggccgg cgtggtcggg gaggcggtgg
     1201 tcgccctggt cctggccgac gccctgctgg agaagaccgg cggcgactcc ctggccgagg
     1261 tccgccgcaa cctcgacgcc tacctgagcg gcctcgaccc ccaggacccc gccggccccc
     1321 agggtgccgg gggatagggg acggcgggcg gcgcggccag gccgcgacgg atccgggcca
     1381 ggtcccgctc cgaccgccac agccggaccc gcagggccag cagcagcatg gtcagcacca
     1441 ccaggaccgt cgccgccacc ccgcggttga cgaccgcgtc ctgcccgaac agggcgagga
     1501 cgccgccgag ctggcgcggc cacagcgaga aggcgacgga cagggcggtg caggagaaga
     1561 acacgagcag caggaaccgg cgcatgacct gacctccccc ggcgtcggtg agcctgaccc
     1621 ggccatggtc ccgcccccgc ccggcctggg ctgtcagccg ccggacagcc gcgccgtcag
     1681 cctccggacg ccggctcggt cctgcacgcc ccgggctgtt aggcttgccg gcatgcgccc
     1741 gatcctgcca accatcgtgg tcatcggctt catgggggtc ggcaagacct cggtcggccg
     1801 gctgatcgcc cgggccctcg gccgcgagtt cgtcgacacc gacctgatga tcgagcgggc
     1861 cgcccgcatg accatcgccg agctgttcca gcgcgagggc gagctgagct tccgccgccg
     1921 cgagatggcc gccatcgacc gggccatctc ggtgcctggc cgggtgatcg cggtcggcgg
     1981 cggcgccgtg ctctcggccg agaaccgcac cgccctcaag caggccgggc tgctggtcta
     2041 cctgcgggcc acgcccgaga ccctggccgg ccgcctgacc ggggtcaccg accggccgct
     2101 gctcggcgac accgccgacc gggccggccg catccgcgac ctgctggtgg cccgcgggcc
     2161 gatctacgag acggccggcg acgtggccat cgacaccgac cggctcaacg tggagcaggt
     2221 tgccgtggag gtcatggagt ggtaccgccg ccgggtcggg gccatggccg gccggtgaca
     2281 acggcccagc cggcaggcgg tgcggttcct tgacgccgga tcggcccggg agcgtcctgc
     2341 cggcgagggt cctcgtcccc accggcgggg gcggctaccc cgtcctcatc ggcggcgggc
     2401 tgctggaccg gctcgacgac ctgc
//
LOCUS       DASSWR010000391         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_411044,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000391 DASSWR010000000
VERSION     DASSWR010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411044"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFO47_06460"
     CDS             complement(<1..692)
                     /locus_tag="VFO47_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8788372.1"
                     /translation="MAGAGISGERLDVLIVGAGPTGLTLAGQLQALGASFRLVDRQAD
                     RVHESRALAVQPRTLEVLRGLDVTGELLARGNDAVWLRLQAGGRAVRVRLFGLGLADT
                     AYPFLLFVPQAETEAVLGDRLAAGGGRVERSVELTGFSAGADRVACTLRHPGGRTEQV
                     DARYLVGCDGAASGVRRGAGIPFRGGAYPQTFALADLEIDGDLETDSAYSFLGDESIL
                     FCFPLGRPATWRL"
     gene            721..1722
                     /locus_tag="VFO47_06465"
     CDS             721..1722
                     /locus_tag="VFO47_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8788373.1"
                     /translation="MDARGRACPLAYRYQPEALAQPPPLEAGTLLVVGGLYGNLPALQ
                     TVLQRAGQEPGRATVVFNGDFHWLDVGPEDFQAIGEMVLAHHAIKGNVEAELASSEDA
                     GCGCAYPGYVGDDVVDRSNQIMTRLRDTARQFPSLVHRLGELPRYLTASVAGHRIGIV
                     HGDPESLAGWRLALEAMEPGDPEVRRQVGWRGRPTTPADVLDWFRRADVDVFASTHTG
                     LPYAQALADGGRRRLVVNNGAAGLGNFAGTSYGVITRLSSDPRPPADSLYGATIGALR
                     CDALPVPFDLDAWKARFLAQWPPGSPAHRSYLTRITGGTHLQPRQAARAGVTLTATA"
     gene            1785..2030
                     /locus_tag="VFO47_06470"
                     /pseudo
     CDS             1785..2030
                     /locus_tag="VFO47_06470"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            2190..2813
                     /locus_tag="VFO47_06475"
     CDS             2190..2813
                     /locus_tag="VFO47_06475"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET8788374.1"
                     /translation="MDPRTGQTFARSIESLAEEFRGIFSLETIERSVNEAIDRLSGAR
                     VVDLIPLFVHRFSRERLRPLAQAEGAIINDVPEVLFVCVHNAGRSQMAAALLDHHAKG
                     RVHVLSAGSDPGDKINPAAVAAMDEVGIDLSREFPKPLTDEFVKAADVVVTMGCGDAC
                     PIYPGKRYEDWELDDPAGQPVEVVRRIRDDIDARVRQLLVELVPAGA"
     gene            2951..3148
                     /locus_tag="VFO47_06480"
     CDS             2951..3148
                     /locus_tag="VFO47_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788375.1"
                     /translation="MRYRIVVRGELSQRYSAAFEGMSLLAEDGQTVITGPVVDQAHLH
                     GLLDRIGDLGLELVSVNEARR"
     gene            3152..>3590
                     /locus_tag="VFO47_06485"
     CDS             3152..>3590
                     /locus_tag="VFO47_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788376.1"
                     /translation="MGAPQPAALAGLPAGPAGGRGRARGSRLRSPDGRPAGETGLPLL
                     ATKLAPPRPPVRLVSRPRLLGLLDAGTRQLLTVVSGPAGAGKTTLLTSWIAAGQPPGP
                     VAWLSLDPGDDEPARFWAYLQAALRRSGAVPAGSTLRSLAPRPG"
BASE COUNT          482 a         1412 c         1238 g          458 t
ORIGIN      
        1 agccgccagg tcgcgggccg gcccaggggg aagcagaaca ggatgctctc gtcgccgagg
       61 aagctgtagg cgctgtcggt ctcgaggtcg ccgtcgatct ccagatcggc cagggcgaag
      121 gtctgggggt aggcgccgcc ccggaacggg atcccggcgc cgcggcgcac gccgctggcg
      181 gcgccgtcgc agccgaccag gtagcgggcg tccacctgct cggtgcggcc gccgggatgc
      241 cgcagggtgc aggcgacccg gtcggcgccg gcgctgaagc cggtcagctc gacgctccgc
      301 tcgacccgcc cgccgccggc ggccagccgg tcgccgagga ccgcctcggt ctcggcctgg
      361 ggcacgaaca gcaggaacgg gtaggcggtg tcggccagac cgagtccgaa cagccggacc
      421 cgcacggccc ggccgcccgc ctgcagccgc agccacaccg cgtcgttgcc ccgcgccagc
      481 agctccccgg tcacgtccag cccgcgcagg acctccaggg tgcgcggctg gacggccagg
      541 gcccgcgact cgtgcacccg gtcggcctgc cggtccacga gccggaagga cgcccccagc
      601 gcctgcagct gcccggccag ggtgagcccg gtcggcccgg ccccgacgat caggacgtcc
      661 agccgttcgc cgctgatgcc cgcaccggcc atggcggcga tactacgctc tggagctgcc
      721 atggacgctc ggggacgcgc ctgcccgctg gcctaccggt accagccaga ggcgctcgcc
      781 cagccaccac cgctggaggc cggcaccctg ctcgtcgtcg ggggtctcta cggcaacctg
      841 ccggccctcc agaccgtgct ccagcgggcc ggacaggaac ccggccgggc gacggtcgtg
      901 ttcaacggcg acttccactg gctggacgtg gggcctgagg acttccaggc gatcggcgag
      961 atggtcctgg cccaccacgc gatcaagggc aacgtcgagg cggagctcgc ctcctcggag
     1021 gacgccggct gcggctgcgc ctaccccggc tatgtcggcg acgacgtcgt cgatcgctcc
     1081 aaccagatca tgacccggct ccgggacacg gcccggcagt tcccgagcct ggtgcaccgc
     1141 ctcggcgagc tgccccggta cctgacggcc agcgtggccg ggcaccgcat cgggatcgtc
     1201 cacggggatc ccgagtcgct ggccggctgg cgcctggccc ttgaggcgat ggagcccggc
     1261 gacccggagg tccggcgcca ggtcggctgg cgtggccggc cgacgactcc cgccgacgtg
     1321 ctggactggt tccgccgcgc cgacgtcgac gtcttcgcca gcacccacac cgggctcccc
     1381 tacgcccagg ccttggccga cggcggtcgc cggcgcctgg tcgtcaacaa cggcgccgcc
     1441 gggctcggca acttcgccgg aaccagctac ggcgtcatca cccggctctc gagcgacccc
     1501 cgaccacccg ccgacagcct gtacggcgcc accatcgggg cgctccgctg cgacgccctg
     1561 ccggtcccct tcgacctcga cgcctggaag gcccgcttcc tcgcccagtg gccccccggc
     1621 agccccgccc accgctccta cctcacccgc atcaccggcg gcacccacct ccagccgcgg
     1681 caggccgcac gagccggcgt cacgctcacc gccacggcct gaccagcgac gcgatggcac
     1741 ccgccccttg aagccgccgg ttgtatcagt caatattgat ctagatgacc gccacggcgc
     1801 cgccagccac gccagctccc gccctcgagg tcgctcgggc gctggctgac ccgttgcgct
     1861 ggcgcatcgt ggagctgctg gccggcgagc aactgtgcgt cgcccacctc accgaggagc
     1921 tcggggtcgg ccagccgctg gtcagccacc acctcaagac gctccggacc gccgggctgg
     1981 tcacctgcga ccgctaccgc tactggacct actaccggct ccaccccgac gccttacggc
     2041 tgctggccgg ccacctcgac ggcctggccc gcatggcccc ggctggggtg gcgtgccggc
     2101 gtgcaatcga cggccaggcc ccgccaaccc ccgggaaccg ccccaccgga tcccgaggag
     2161 gcgggccgat gccgctccac gaccagccca tggacccgcg caccggccag accttcgccc
     2221 gctccatcga gtcgctggcc gaggagttcc gcggcatctt ctccctcgag accatcgaac
     2281 gctccgtgaa cgaggccatc gaccggctgt cgggcgctcg ggtggtcgac ctcatccccc
     2341 tgttcgtgca ccggttctcc cgcgagcggc tgcggcccct cgcccaagcc gaaggagcca
     2401 tcatcaacga cgtgcccgag gtcctgttcg tctgtgtcca caatgccggc cgcagccaga
     2461 tggcggctgc cctgctcgac caccacgcca agggcagagt tcatgtcctc tcggccggca
     2521 gcgaccccgg cgacaagatc aacccggccg cggtggcagc gatggacgag gtcgggatcg
     2581 acctgtcccg ggagttcccc aagccgctca ccgacgagtt cgtcaaggcg gccgacgtgg
     2641 tcgtcaccat gggctgcggc gacgcctgcc cgatctaccc gggcaagcgc tacgaggact
     2701 gggagctgga cgacccggcc ggccagccgg tcgaggtggt ccgccggatc cgcgacgaca
     2761 tcgacgcccg cgtgcggcag ctactggtgg agctcgtgcc agccggcgcc tgagcacctg
     2821 accaggagca cgctggtcgc catctcaccc cggtgaactc atcggggtga tgaggccgtc
     2881 tcatccacct ttggtggagc gtggcggggt gatgccaccc aacccgtgcc gtcctcccag
     2941 gcggcgaccg atgcgctacc ggatcgtcgt ccgcggggag ctgagccagc gctactcggc
     3001 ggcgttcgag ggcatgagcc tgctggccga ggacggccag accgtgatca ccggtccggt
     3061 ggtcgaccag gcgcacctcc acggcctgct cgaccggatc ggcgatctcg gcctggagct
     3121 ggtcagcgtc aacgaagcga ggaggtaggc gatgggcgcg ccgcagcccg ccgcgctcgc
     3181 cggcctgccg gccggtccgg ccggcgggag agggcgcgcg cgagggagcc ggctgcggtc
     3241 gcccgatggg cgccccgccg gcgagaccgg gctgccgctg ctcgccacca agctcgcgcc
     3301 gccacgccca ccggtgcgtc tggtgtcccg gccccggctg ctcgggctgc tcgatgccgg
     3361 gacccgccag ctgctgaccg tggtctcggg cccggccggt gcgggcaaga cgacgctgct
     3421 gacctcctgg atcgcggccg gccagcctcc cggtccggtg gcctggctgt cgctggaccc
     3481 cggcgacgac gagcctgccc gcttctgggc gtacctgcag gcggcgttgc gccggtccgg
     3541 cgccgtcccg gccggcagca cgctgcgatc gctggcgccg cgacccggac
//
LOCUS       DASSWR010000392         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_495553,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000392 DASSWR010000000
VERSION     DASSWR010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495553"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..624)
                     /locus_tag="VFO47_06490"
     CDS             complement(112..624)
                     /locus_tag="VFO47_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788377.1"
                     /translation="MDGVVRGRGRGGVAARPLGRPLDRLAWVHAISTLGDALVAVALA
                     GTLFFAVPVAAARPRVALYLLLTVAPFAVVAPLLGRLLDGRSGAGRLALAAAMALRAA
                     LAALAVSRTESLLLYPLAFGLLVCSRAHGISRTAMVPELLGPEGSGRTGQISGGVPRS
                     VGTTSRTWSP"
     gene            complement(701..>3002)
                     /locus_tag="VFO47_06495"
     CDS             complement(701..>3002)
                     /locus_tag="VFO47_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET8788378.1"
                     /translation="EIIGQGQSAPTGHIPFTPRAKKVLELSLREALQLGHNYIGTEHI
                     LLGLIREGEGVAAQVLVKLGADLSRVRQQVIQLLSGYAGGKEGQGGGAPGEAQPQGSL
                     VLDQFGRNLTQLAREGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLSQAIVKGEVPETLKGKQMYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRGDIVLF
                     IDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLEEYRKHLEKDAALERRFQ
                     PIQVQEPSVAHTIDILKGLRDRYEAHHRVTITDDALVASANLADRYISDRFLPDKAID
                     LIDEAGSRMRIRRMTAPPDLREFDGRIADVRRDKESAIDAQDFEKAASLRDTEKNLLN
                     DKARREREWKSGDMDVVAEVDEEQIAEVLASWTGIPVFKLTEEETAKLLRMEDELHKR
                     IVGQDNAIHAVSQAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELSRTLAEFLFGDED
                     ALIALDMSEYMEKHTVSRLIGSPPGYIGYEEGGQLTEAVRRKPFSVVLFDEIEKAHPD
                     VFNTLLQILEDGRLTDAQGRTVDFKNTVLIMTSNLGTRDIAKGATLGFTARPDAKVDY
                     ERMQSKVMDELKRHFRPEFLNRIDEVIVFHQLSREQVKSIVDLMMRRVVEQLKSKDIA
                     MELTEAAKDLMAEKGYDPALGARPLRRTIQRLVEDPLSEKLLWKEFTAGQLVVVDAEN
                     GEIAFRSLDRPSEEIDVPPAELAGKE"
BASE COUNT          393 a         1026 c         1117 g          466 t
ORIGIN      
        1 tgagggtggc ggcccagagg acggcgccgc cgccgaggag gcggtcgagg cccacgccga
       61 ggagggcgcc ggcggtgccg ccgagggcgg ccactctggc catgcggccg ttcacggcga
      121 ccaggtcctg ctggtcgtcc cgaccgaccg gggcacccct ccggagatct gccctgtccg
      181 tccggacccc tccgggccga gcagctcggg gaccatggcc gtgcggctga tgccgtgggc
      241 ccgggagcag accaggaggc cgaaggcgag cgggtagagg agcagggact cggtccggga
      301 cacggccagg gcggccaggg cggcccgcag ggccatggcg gcggccaggg ccaggcggcc
      361 ggcgccgctg cggccgtcca gcaggcggcc gagcaggggc gccaccaccg cgaacggggc
      421 gaccgtcaac aggaggtaga gcgcgacccg gggccgggcc gcggccaccg gcacggcgaa
      481 gaacagggtc ccggccaggg ccaccgcgac cagggcgtcg cccagggtgc tgatcgcgtg
      541 cacccaggcc agccggtcca gcggccggcc cagcggccgg gcggccacgc ccccccgacc
      601 ccggccccga accaccccgt ccatgtgcgg catgctaacg gcgaagcccc cggggatccc
      661 gggggcttcg cctatccctg gggtgggaca gctgggacgc ctactccttg ccggccagct
      721 cggccggggg gacgtcgatc tcctcggaag gccggtcgag cgagcggaac gcgatctcgc
      781 cgttctcggc gtcgaccacg accagctggc cggccgtgaa ctccttccac agcagcttct
      841 cggacagcgg gtcctcgacc aggcgctgga tggtccgccg cagcgggcgg gccccgaggg
      901 ccgggtcgta gcccttctcg gccatcaggt ccttggccgc ctcggtcagc tccatggcga
      961 tgtccttgga cttgagctgc tccaccaccc gccgcatcat caggtcgacg atgctcttca
     1021 cctgctcgcg cgagagctgg tggaagacga tgacctcgtc gatccggttg aggaactccg
     1081 gccggaagtg gcgcttgagc tcgtccatga ccttggactg catccgctcg tagtcgacct
     1141 tggcgtcggg ccgggcggtg aagcccaggg tggcgccctt ggcgatgtcg cgggtgccca
     1201 ggttggaggt catgatcagc acggtgttct tgaagtcgac cgtgcggccc tgggcgtcgg
     1261 tcagccgccc gtcctccagg atctggagga gggtgttgaa cacgtccggg tgggccttct
     1321 cgatctcgtc gaacagcacc acgctgaacg gcttgcgccg gaccgcctcg gtgagctggc
     1381 cgccctcctc gtagccgatg tagccgggcg gggagccgat aagccgcgag accgtgtgct
     1441 tctccatgta ctccgacatg tcgagcgcga tcagcgcgtc ctcgtctccg aacaggaact
     1501 cggccagggt gcgggacagc tcggtcttcc cgacgcccga ggggccgagg aagatgaacg
     1561 agcccgacgg ccgcttgggg tccttgaggc cggccctggt gcggcggatg gcctgggaga
     1621 cggcgtggat ggcgttgtcc tggccgacga tccgcttgtg cagctcgtcc tccatgcgca
     1681 ggagcttggc cgtctcctcc tcggtcagct tgaacaccgg gatgccggtc caggaggcca
     1741 gcacctcggc gatctgctcc tcgtcgacct cggcgaccac gtccatgtcg cccgacttcc
     1801 actcccgctc gcggcgggcc ttgtcgttga ggaggttctt ctcggtgtcg cggaggctgg
     1861 ccgccttctc gaagtcctgg gcgtcgatcg ccgactcctt gtcgcgccgg acgtcggcga
     1921 tgcggccgtc gaactcgcgg aggtcgggcg gggcggtcat gcgccggatg cgcatgcggc
     1981 tgccggcctc gtcgatcagg tcgatcgcct tgtcgggcag gaagcggtcg gagatgtagc
     2041 ggtcggccag gttggcggag gcgaccaggg cgtcgtcggt gatggtgacg cggtggtggg
     2101 cctcgtagcg gtcgcgcagc cccttgagga tgtcgatggt gtgggcgacc gacggctcct
     2161 ggacctggat cggctggaag cggcgctcca gggcggcgtc cttctccagg tgcttgcggt
     2221 actcctcgag ggtggtggcg ccgatggtct ggagctcgcc ccgggccagc atgggcttga
     2281 ggatcgaggc ggcgtcgatc gcgccctcgg ccgcgcccgc cccgacgagc gtgtgcagct
     2341 cgtcgatgaa caggacgatg tcgccgcggg tgcggatctc cttgaggacc ttcttgaggc
     2401 gctcctcgaa gtcgccgcgg tagcgggacc cggcgaccag ggcgcccagg tccagggtgt
     2461 acatctgctt gcccttgagc gtctcgggaa cctcgccctt gacgatggcc tgggagaggc
     2521 cctcgacgat ggcggtcttg ccgacgccgg gctcgccgat cagcaccggg ttgttcttgg
     2581 tccggcggga cagcacctgc atgacccgct cgatctcctt ctcgcgcccg atcaccgggt
     2641 cgagcttgcc ctcgcgggcc agctgggtga ggttgcgccc gaactggtcg agcaccaggg
     2701 agccctgcgg ctgggcctcg cccggggcgc cgccgccctg gccctccttg ccgccggcgt
     2761 agccggacag cagctggatg acctgctggc ggacgcggga gaggtcggcg cccagcttga
     2821 ccagcacctg ggcggccacg ccctcgccct cacggatgag cccgagcagg atgtgctcgg
     2881 tgccgatgta gttgtggccg agctgcaggg cctcgcggag cgacagctcc aggaccttct
     2941 tggcccgcgg cgtgaacggg atgtgcccgg tgggcgcgct ctggccctgg ccgatgatct
     3001 cc
//
LOCUS       DASSWR010000393         5056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1314955, whole genome shotgun sequence.
ACCESSION   DASSWR010000393 DASSWR010000000
VERSION     DASSWR010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314955"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFO47_06500"
     CDS             complement(<1..906)
                     /locus_tag="VFO47_06500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET8788379.1"
                     /translation="MTSAPEHELLAAARGDDGEAFGRLVAPYRAELHAHCYRMLGSVQ
                     DAEDALQEALLGAWRGLHGFEGRSSLRAWLYRITTNACLRLGSRRPRRLLSADYGPAF
                     TQTDDLGEPVGEPVWLEPFPTPEGRYELRESVELAFVAALQHLPATQRAVLILREVLG
                     FSAAEVAGILDTTPASVNSALQRARKAVDERVPHPSQQAELDALGDGGRRELVDALMA
                     AWERADVAALLELLADDARFMMPPLPAWFAGRAAIGRFLAGRMFATPWRLAPLEANGQ
                     LAVACYQGDPATGRFRLSAVNVLSVR"
     gene            complement(903..2393)
                     /locus_tag="VFO47_06505"
     CDS             complement(903..2393)
                     /locus_tag="VFO47_06505"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET8788380.1"
                     /translation="MPRLALSPVRPERADADLLVLPVAAGEAGPAAPPVTAAVLATLG
                     ADLAAVAGGTGRFTGALDDVLLLPAYGAVAAPAVLLVGVGPDADRNAETLRRAAAVAV
                     GAAGKAATMATALHQAGVPGADGVALAAVAEGLVLAAYRFQRHKSAPDPDLAAATLLV
                     DGDAELAVAEPVLHRAEVAARATLAARDLVNEPASRINPATLAAEAVRLAKAAGLEHE
                     VLTGPALRRGRFGAVLAVGGGSATGPRLVRLRYRPPGAGRHVALVGKGVTFDTGGIDL
                     KRGASMDGMKDDMAGAAAILAAVAAAAELELPTAVTAVLPLVENMPGASAMRPGDVVT
                     ARNGVTVEVTNTDAEGRLILADGLALAAEDGPDAILDLATLTGSVVHGLGLGCTGVFG
                     NTPALTAEVLAAAARAGELACELPMIEDYRRFLDSEVADLVNATNEPGDSVQAALFLR
                     EFVAGTPWVHLDIAGPATAKEARYYQPKGASGWGVRTLLAFLEGPR"
     gene            2458..2859
                     /locus_tag="VFO47_06510"
     CDS             2458..2859
                     /locus_tag="VFO47_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788381.1"
                     /translation="MWRRRGPDPAVEAAWAGFLGFAKALDEGIRALLATIPSARRPGA
                     PLDAGVAGFLAGLDAARAELPAWDHPALADAQAATAAALDAAEGRARDLPAASQGLEF
                     EHRNERVAEVIDELLPVQEAERAVRDLRRRR"
     gene            complement(2856..3671)
                     /locus_tag="VFO47_06515"
     CDS             complement(2856..3671)
                     /locus_tag="VFO47_06515"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET8788382.1"
                     /translation="MNRPDVHDDHGGWRELAEGLHGLRAEPPPTLAPAVLERVGLADA
                     FVTVHGPAGPLLVAYNGRGVSATAPGGDEAGFRERFQARFRRPLRPADQAPAKLVAQV
                     ERVLDPGRAGHPPRYDLRGTSEFERAVLAKAQEIPRGQVRPYAWVAGELGQPAAVRAV
                     GAALGRNPIPVLIPCHRVVGSDGRLTGYAWGVPYKRTLLAAEGADPDELERLGRRGTR
                     FFGSDTTRIYCYPTCANARRVSDRHLMNFRDVAQATAAGYRPCRVCRPPALAG"
     gene            complement(3668..4261)
                     /locus_tag="VFO47_06520"
     CDS             complement(3668..4261)
                     /locus_tag="VFO47_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8788383.1"
                     /translation="MFKPVMEPQPELPARLARDLDGSFAELVGAYQRLVFGLALRVVA
                     DRADAEEVAQDTFARAYWALAGYPAERVAAMRLRPWLAQIALNQARNRLRRRPPPARP
                     LEDGDGQPLAVAAPAAAEPAAVAERGQDRELLVELLAALPRGWREAVVLRHVEGLPYA
                     EVAEVLGRPVGTVKTHVHRGVRQLREHLEARTEGVPR"
     gene            complement(4281..>5056)
                     /locus_tag="VFO47_06525"
     CDS             complement(4281..>5056)
                     /locus_tag="VFO47_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HET8788384.1"
                     /translation="RPSLALGVDALRDLNVVDAGDVLMLPGEIEPALERLERAVHTVA
                     AARAIPVVLGGDHTIALPDVTGVARHHGLGRVSVVHFDAHADTGDTQFGSLLGHGTPM
                     RRLIESGAARGDRFLQLGLRGYWPPPDVLAWMADQGMRSFEMTEIVHRGLDACLSEAF
                     AIATDDCDGVFLSVDVDVVDPGMAPGTGTPEPGGLTARELLDAVRRCAVELPVVGMDV
                     VELSPAYDGPGQVTAFLANRVVLEALSGIARRRASAAEG"
BASE COUNT          583 a         1914 c         2014 g          545 t
ORIGIN      
        1 gcgcacgctg agcacgttga cggcgctgag ccggaaccgg cccgtggccg ggtcgccctg
       61 gtagcaggcg acggccagct ggccgttggc ctccagcggg gccagccgcc agggggtggc
      121 gaacatccgc ccggccagga accggccgat ggcggcccgg ccggcgaacc aggcgggcag
      181 gggcggcatc atgaaccggg cgtcgtcggc cagcagctcc aggagggcgg ccacgtcggc
      241 ccgctcccag gcggccatca aggcgtcgac cagctcgcgc cggccgccgt cgccgagggc
      301 gtccagctcg gcctgctggg aggggtgcgg gacccgctcg tcgaccgcct tgcgggcccg
      361 ctgcagggcg ctgttcaccg acgccggggt ggtgtcgagg atgcccgcca cctcggcggc
      421 cgagaacccc agcacctccc gcaggatgag cacggcccgc tgggtcgccg ggaggtgctg
      481 gagggcggcc acgaaggcca gctcgacgct ctcgcgcagc tcatagcggc cctcgggggt
      541 ggggaacggc tccagccaca ccggttcgcc caccggctcg ccgaggtcgt cggtctgggt
      601 gaaggcgggc ccgtagtcgg ccgagagcag ccgccggggc cgccgggacc ccagccgcag
      661 gcacgcgttg gtggtgatcc ggtacagcca ggcccgcagc gagctgcggc cctcgaaccc
      721 gtgcaggccc cgccaggcgc cgagcagcgc ctcctggagg gcgtcctcgg cgtcctggac
      781 cgacccgagc atcctgtagc agtgggcgtg cagctcggcc cggtagggcg ccaccagccg
      841 gccgaacgcc tccccgtcgt cgccccgggc cgccgccagc agctcgtgct ccggggccga
      901 ggtcaccgcg gcccttccag gaacgccagc agggtccgga cgccccagcc gctggccccc
      961 ttgggctggt agtagcgggc ctccttggcg gtggccgggc cggcgatgtc gaggtggacc
     1021 cagggggtgc cggccacgaa ctcgcgcagg aacagcgccg cctggacgct gtcgcccggc
     1081 tcgttggtgg cgttgaccag gtcggcgacc tcggagtcga ggaagcggcg gtagtcctcg
     1141 atcatgggca gctcgcaggc cagctcgccg gcgcgggcgg cggcggccag gacctcggcg
     1201 gtgagggccg gggtgttgcc gaacacgccc gtgcagccga gccccagccc gtgcaccacc
     1261 gagccggtca gggtggccag gtcgaggatg gcgtcggggc cgtcctcggc ggccagggcg
     1321 aggccgtcgg ccaggatcag gcggccctcg gcgtcggtgt tggtcacctc cacggtgacg
     1381 ccgttgcggg cggtgacgac gtcgccgggc cgcatcgccg aggccccggg catgttctcg
     1441 accaggggca ggaccgcggt cacggccgtg ggcagctcca gctcggccgc ggccgccacg
     1501 gcggccagga tcgccgccgc cccggccatg tcgtccttca tcccgtccat gctggcgccg
     1561 cgcttgaggt cgatcccgcc ggtgtcgaag gtcacgccct tgccgaccag ggccacgtgc
     1621 cggccggccc cgggcggccg gtagcggagc cggaccaggc gggggccggt ggccgacccg
     1681 ccgccgacgg ccaggacggc cccgaagcgg ccccggcgca gggccggccc ggtcagcacc
     1741 tcgtgctcca ggccggccgc tttggccagg cggaccgcct cggcggccag ggtggccggg
     1801 ttgatgcggc tggccggctc gttgaccagg tcgcgggcgg ccagggtggc ccgggcggcg
     1861 acctcggccc ggtgcagcac cggctcggcc acggccagct cggcgtcgcc gtcgaccagc
     1921 agggtggccg cggccaggtc ggggtcgggg gccgacttgt gccgctggaa ccggtaggca
     1981 gccagcacga gcccctcggc cacggcggcc agcgcgacgc cgtcggcgcc ggggacgccg
     2041 gcctggtgga gggcggtggc catggtcgcg gccttgccgg cggcgcccac ggccacggcg
     2101 gcggcccggc gcagggtctc ggcgttccgg tcggcgtcgg gcccgacccc gaccagcagc
     2161 accgccgggg cggccacggc cccgtaggcg ggcagcagca gcacgtcgtc cagggccccg
     2221 gtgaaccgcc cggtcccgcc ggccacggcg gccaggtccg ccccgagggt ggccagcacg
     2281 gccgcggtca ccggcggggc ggccgggccg gcctcgccgg cggccaccgg cagcaccagc
     2341 aggtcggcgt cggcccgctc gggccggacg ggggacaggg ccaggcgcgg catcgtctgc
     2401 tccgtgtggg tatcgtcggg tcggcggcgg gagccaatcc taggggaggt ggcggccatg
     2461 tggaggcggc gggggcccga cccggccgtg gaggcggcct gggccgggtt ccttggtttc
     2521 gccaaggccc tcgacgaggg gatccgggcg ctgctggcca ccatcccctc ggcccgccgg
     2581 cccggggcgc cgctggacgc gggcgtggcc gggttcctgg ccggcctgga cgcggcccgg
     2641 gccgagctgc ccgcctggga ccacccggcc ctggccgacg cccaggcggc caccgccgcc
     2701 gccctcgacg ccgccgaggg ccgcgcccgc gacctcccgg cggccagcca gggcctggag
     2761 ttcgagcacc gcaacgagcg ggtcgccgag gtcatcgacg agctcctgcc cgtgcaggag
     2821 gccgagcggg ccgtgcggga cctgcgccgg cgccgctagc cggccagggc cggcggccgg
     2881 cagacccggc agggccggta gccggcggcg gtggcctggg ccacgtccct gaagttcatc
     2941 aggtggcggt cgctgacccg gcgggcgttg gcgcaggtcg ggtagcagta gatccgggtg
     3001 gtgtcgctgc cgaagaagcg ggtgccgcgg cggccgaggc gctccagctc gtcggggtcg
     3061 gcgccctcgg cggcgagcag ggtccgcttg taggggacgc cccaggcgta gccggtcagg
     3121 cggccgtcgc tgccgaccac ccggtggcag gggatcagca ccgggatcgg gttgcggccc
     3181 agggcggcac cgaccgcccg cacggccgcc ggctggccga gctcgccggc cacccaggcg
     3241 taggggcgga cctggccgcg ggggatctcc tgggccttgg cgagcacggc ccgctcgaac
     3301 tcggaggtgc cccgcaggtc gtagcggggc gggtggccag cgcgcccggg gtcgaggacc
     3361 cgctcgacct gggcgaccag cttggccggg gcctggtcgg ccggccgcag cggccgccgg
     3421 aaccgggcct ggaaccgctc ccggaacccg gcctcgtcgc cgcccggggc ggtggccgag
     3481 acgccccggc cgttgtaggc gaccagcagc ggcccggccg gcccgtgcac ggtcacgaac
     3541 gcgtcggcca ggccgacccg ctccagcacc gccggggcca gggtcggcgg cggctcggcc
     3601 cgcaggccgt gcaggccctc ggccagctcc cgccagccgc cgtggtcgtc gtggacgtcg
     3661 ggtcggttca tcgtggcacc ccttccgttc gggcctccag gtgttcccgt agctgccgca
     3721 ccccccggtg cacgtgggtc ttgaccgtcc ccaccggccg gccgaggacc tcggcgacct
     3781 cggcgtaggg aaggccctcg acgtggcgca gcaccaccgc ctcgcgccag ccgcggggca
     3841 gggcggccag cagctcgacc agcagctcgc ggtcctggcc gcgttcggcc accgcggccg
     3901 gctcggcggc ggcgggggcg gccacggcca ggggctggcc gtcgccgtcc tccagcggcc
     3961 gggccggcgg cggtcgccgc cgcagccggt tgcgggcctg gttgagggcg atctgagcca
     4021 gccacggccg gagccgcatc gccgccaccc gctcggccgg gtagccggcc agggcccagt
     4081 aggcccgggc gaaggtgtcc tgggcgacct cctcggcgtc ggcccggtcg gccacgaccc
     4141 gcagggccag gccgaagacc agccgctggt aggcgccgac cagctcggcg aacgacccgt
     4201 cgaggtcgcg ggccaggcgg gccggcagct ccggttgtgg ttccatcacc ggcttgaaca
     4261 ccgccccggc cggaggagtt tcacccctcg gccgccgagg cccggcgccg ggcgatcccc
     4321 gacagggcct ccagcaccac ccggttggcc aggaacgccg tcacctgccc gggcccgtcg
     4381 taggcggggg acagctcgac cacgtccatg ccgaccaccg gcagctcgac ggcgcaacgg
     4441 cggaccgcgt ccagcagctc ccgggccgtc agcccgcccg gctccggggt gccggtgccg
     4501 ggggccatgc ccgggtcgac cacgtcgacg tcgaccgaca ggaacacccc gtcgcagtcg
     4561 tcggtggcga tcgcgaacgc ctccgacagg caggcgtcga ggccgcggtg gacgatctcg
     4621 gtcatctcga agctgcgcat gccctggtcg gccatccagg cgagcacgtc cgggggcggc
     4681 cagtagccgc gcagccccag ctggaggaac cggtcgccgc gggccgcgcc cgactcgatc
     4741 aggcgccgca tgggggtgcc gtggccgagc agggagccga actgggtgtc gccggtgtcg
     4801 gcgtgggcgt cgaaatggac caccgacacc cgccccaggc cgtggtggcg ggccaccccg
     4861 gtcacgtcgg gcagggcgat ggtgtggtcg ccgcccagca ccaccgggat cgccctggcc
     4921 gcggccacgg tgtggacggc ccgctccagc cgctccaggg ccggctcgat ctcgccgggc
     4981 agcatcagga cgtcgccggc gtcgaccacg ttgagatccc gcagggcgtc cacgcccagg
     5041 gccagggagg gccggg
//
LOCUS       DASSWR010000394         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_354792,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000394 DASSWR010000000
VERSION     DASSWR010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354792"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VFO47_06530"
     CDS             <1..1471
                     /locus_tag="VFO47_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HET8788385.1"
                     /translation="RFTPNEVYPVVYRRGRAAHLDIGGARGQAIRRALADQLGLDIEE
                     VKPFGLSGSAGSTPLRIKVAGDPPTVLFGKLYARSHLRADRWYKLGRELLYGRLEDEK
                     PFHTVRRLVQQEDYALHKLHLAGLPSPRPYGFVELTPDREYLLVAEFFDRTVELGEAE
                     VDEGVIDDGLGIVRKLWDAGLAHRDVKPANLLVRDGRMLLIDVAFMEARPSPWRQAVD
                     LANMMLCLALRSSPERVYRRALQYFTVEEITEAFAATRGLAMPSQLRHMLRAQGRDLH
                     AEFLDLLPERPAPISNQRFSLRRFGLALMVLIALLVVVPLFVQWAVQTDRTNSSLYTS
                     DVRCSDQEALWLMAQAVPTAAAVPCVQLDPAGWSLNDVKAGAGFASIVFDIVQPFQEA
                     AVTVDLLPSCDLAGATEISSEQPAARRYIRIDRSASPTRVTRVYTFEGGCISERFVSD
                     LSPERLASEASSTFGFVTRSQLARDLSRRSAGRLQLDPP"
     gene            1481..2155
                     /locus_tag="VFO47_06535"
     CDS             1481..2155
                     /locus_tag="VFO47_06535"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET8788386.1"
                     /translation="MGSYEDTVRTLEVELAQVEQAFRGLGDAEWATPTKLRPLDETKP
                     HWTLFELAGHFDISIGLTVMLVAEPQDGQVGRDRVSFFIFPRSEVAPVVYDYAYTMVE
                     GKTPAQMPDVLAATFAKTIEGARSMAPDTVGPGYYALMRLDEFVASRVVEAVVHGLDL
                     TDALGREPMATPEGVAVTAAILDELLARKTVAGRPPDLGDDLAWVRAASGRGPEHPDP
                     RLPLIG"
     gene            2166..3029
                     /locus_tag="VFO47_06540"
     CDS             2166..3029
                     /locus_tag="VFO47_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET8788387.1"
                     /translation="MDFTEDTATGAVEASFAGTPDPRLRELLGSLVRHLHGFVREVEP
                     TFEEWERAIGFLTATGQRCDDTRQEFILLSDVLGVTMLVDAINHRKASEATESTVLGP
                     FHMVSSPPRELGDSIDLVATGQPCVVTGRVVSLDGAPLAGAQVDVWQADDHGFYDVQQ
                     PGTQPPGNGRGLFTCDGDGRFWFRTVTPSAYPIPTDGPVGRLLTATGRHPFRPAHIHF
                     IVAAGGHVPVTTHMFVAGSPYLDSDAVFAVKQSLVRDFTEVDDPAEAARYGVDPPFRH
                     AHFEVVLQPAG"
     gene            3112..>3493
                     /locus_tag="VFO47_06545"
     CDS             3112..>3493
                     /locus_tag="VFO47_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788388.1"
                     /translation="MAQNAEALASFLPGRLVRRLVEAPEEAGLPHADRMVAALLLADI
                     SGFTAITERLAERGPGGAEELRGLLDGVFQPLLELVAGTGGDVLKFAGDALLACWPAP
                     AGDLAERGLAGATATAAGCAEAMQA"
BASE COUNT          441 a         1336 c         1237 g          479 t
ORIGIN      
        1 ccgcttcacc cccaacgagg tctacccggt cgtctaccgg cgcggccggg ccgcccacct
       61 ggacatcggc ggggcccgcg gccaggccat ccggcgcgct ctggccgacc agctcggcct
      121 ggacatcgag gaggtcaagc cgttcgggct ctcgggctcg gccgggtcga cgcccctgcg
      181 catcaaggtc gccggcgacc cgccgacggt cctgttcggc aagctgtacg cccgcagcca
      241 cctgcgcgcc gaccgctggt acaagctggg ccgcgagctg ctctacggcc gcctggagga
      301 cgagaagccc ttccacaccg tccgccgcct cgtccagcag gaggactacg ccctccacaa
      361 gctgcacctg gccgggctgc cgtcgccccg gccctatggc tttgtcgagc tcacccccga
      421 ccgcgagtac ctgctggtcg ccgagttctt cgaccgcacg gtggagctcg gcgaggccga
      481 ggtcgacgag ggcgtgatcg acgacggcct cggcatcgtc cgcaagctgt gggacgccgg
      541 cctggcccac cgtgacgtca agccggccaa cctgctggtc cgtgacggcc gcatgctgct
      601 gatcgacgtg gccttcatgg aggcccggcc cagcccctgg cgccaggccg tcgacctggc
      661 caacatgatg ctgtgcctgg ccctgcggtc cagccccgag cgggtctacc ggcgggccct
      721 gcagtacttc acggtggagg agatcaccga ggcgttcgcg gccacccgcg gcctggccat
      781 gccgtcccag ctccgccaca tgctgcgggc ccagggccgc gacctgcacg ccgagttcct
      841 cgacctgctg cccgaacggc ccgcccccat ctccaaccag cgcttcagcc tgcggcggtt
      901 cgggctcgcg ctcatggtcc tcatcgcgct gctggtcgtc gtgcccctgt tcgtgcagtg
      961 ggcggtgcag accgaccgga ccaactcctc gctgtacacc agcgacgtcc gctgctccga
     1021 ccaggaggcg ctgtggctga tggcccaggc cgtgccgacg gccgccgccg tcccctgcgt
     1081 ccagctcgac ccggccggct ggtccctcaa cgacgtcaag gcgggcgcgg ggttcgccag
     1141 catcgtcttc gacatcgtcc agccgttcca ggaggcggcg gtcaccgtcg acctgctgcc
     1201 ctcctgcgac ctggccgggg cgaccgagat cagctccgag cagccggcgg ccaggcggta
     1261 catccggatc gaccgctcgg ccagccccac gcgggtcacc cgcgtctaca ccttcgaggg
     1321 cggctgcatc agcgagcggt tcgtctccga cctctccccc gagcgcctcg ccagcgaggc
     1381 gtcctccacc ttcggcttcg tcacccgctc ccagctcgcc cgcgacctca gccggcgctc
     1441 cgccggccgc ctgcagctgg acccgccata ggaggcgccc atgggctcgt acgaggacac
     1501 cgtccgcacc ctcgaggtgg agctcgccca ggtcgagcag gccttccggg gcctcggcga
     1561 cgccgagtgg gccaccccga ccaagctccg gccgctcgac gagaccaagc cccactggac
     1621 gctgttcgag ctggccgggc acttcgacat ctcgatcggg ctgaccgtga tgctggtggc
     1681 cgagccccag gacgggcagg tgggccgcga ccgggtgagc ttcttcatct tcccccggtc
     1741 ggaggtcgcg ccggtcgtct acgactacgc ctacaccatg gtcgagggga agaccccggc
     1801 gcagatgccc gacgtcctgg ccgccacctt cgccaagacg atcgaggggg cgcggtcgat
     1861 ggctccggac acggtcggcc ccggctacta cgccctcatg cggctggacg agttcgtggc
     1921 cagccgggtg gtggaggcgg tggtgcacgg cctcgacctc accgacgccc tcgggcgcga
     1981 gcccatggcg acccccgagg gggtcgcggt gaccgcggcc atcctggacg agctgctggc
     2041 ccgcaagacc gtggccgggc ggccgccgga cctgggcgac gacctggcct gggtgcgggc
     2101 ggcctcgggc cgggggccgg agcaccccga tccgaggctg ccgctgatcg gctgaggcgg
     2161 cggccatgga cttcaccgag gacacggcca ccggggcggt ggaggcgagc ttcgccggca
     2221 cgcccgaccc gcggctccgg gagctcctcg gcagcctggt ccggcacctg cacgggttcg
     2281 tgcgcgaggt cgagccgacc ttcgaggagt gggagcgggc gatcgggttc ctgaccgcca
     2341 ccgggcagcg ctgcgacgac acgcgccagg agttcatcct gctgtcggac gtgctcggcg
     2401 tgaccatgct ggtcgacgcc atcaaccacc gcaaggcgtc ggaggcgacc gagtcgaccg
     2461 tgctggggcc gttccacatg gtctcctcgc ccccgcggga gctgggcgac agcatcgacc
     2521 tggtcgccac cggccagccc tgcgtggtca ccggccgggt cgtctccctg gacggcgccc
     2581 cgctggccgg cgcccaggtc gacgtctggc aggccgacga ccacggcttc tacgacgtgc
     2641 agcagccggg aacccagccg ccgggcaacg gccggggcct gttcacctgc gacggcgacg
     2701 gccgcttctg gttccggacg gtcaccccca gcgcctaccc gatccccacc gacggccccg
     2761 tcggccgcct cctgaccgcg accggccgcc accccttccg ccccgcccac atccacttca
     2821 tcgtcgccgc cggcggccac gtcccggtca ccacccacat gttcgtcgcc ggcagcccgt
     2881 acctggactc cgacgccgtg ttcgccgtca agcagagcct ggtcagggac ttcaccgagg
     2941 tcgacgaccc cgccgaggcg gcccgctacg gggtcgaccc gcccttccgc cacgcccact
     3001 tcgaggtcgt gctccagccg gccgggtaac cgccggcccg gccgctccca tatccttggg
     3061 gagccgggtg gccggccgtg ggtcgccggc tggagggggg agctgatcgc cgtggcccag
     3121 aacgctgagg cgctggccag cttcctgcca gggcggctgg tgcggaggct ggtggaggcg
     3181 ccggaggagg cggggttgcc gcacgccgac cggatggtgg cggcgctgct gctggccgac
     3241 atctccgggt tcacggccat caccgagcgg ctggccgagc gggggccggg cggggccgag
     3301 gagctgcggg ggctgctcga cggggtcttc cagccgctgc tggagctggt cgccgggacc
     3361 ggcggggacg tgctcaagtt cgccggggac gcgctgctgg cctgctggcc ggcgccggcc
     3421 ggggacctgg cggagcgggg gctggccggg gcgacggcga cggcggccgg gtgcgccgag
     3481 gcgatgcagg cgg
//
LOCUS       DASSWR010000395         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_157176,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000395 DASSWR010000000
VERSION     DASSWR010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157176"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFO47_06550"
     CDS             complement(<1..487)
                     /locus_tag="VFO47_06550"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET8788389.1"
                     /translation="MREVASSAELRAATDAARQRGAGVGFVPTMGALHAGHRSLLARA
                     RAENAFVVASVFVNPLQFGPSEDLAGYPRDRDADLAVLRAEGTDLAFVPAEGEMWPAP
                     PEVRLQVGGGLAERLEGLVRPGHLDGVATVVAKLLHLAGPCRAYFGQKDAQQLAVVRR
                     MV"
     gene            complement(462..1403)
                     /locus_tag="VFO47_06555"
     CDS             complement(462..1403)
                     /locus_tag="VFO47_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HET8788390.1"
                     /translation="MPPSQSSKLSPAAVIGPGRVGLALATFLDRIGVEVTMGARSDRG
                     RERVEAAGLAVTDPTAATRGASLVLLAVPDDQLPSLVTALAADGAFAAGQVVVHTAGV
                     DGPGLLAPAAAAGARTAACHPVQIFNDDLEATLGRLPGTVWGVTGDPAGKEVVTTLGG
                     RPMDVPESGRVRYHAALVLAANGCAALSATATDQLRAGGVIDPAALLSGLIHASVDSA
                     LATGQAGLSGPWVRGDGRTVAMHTEALARKQKVINVYVALARLVADRAAAAGRLDPEA
                     RARVEAGLHGHVEGEESHERLELLRRLGRRARGREQR"
     gene            complement(1424..2140)
                     /locus_tag="VFO47_06560"
     CDS             complement(1424..2140)
                     /locus_tag="VFO47_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8788391.1"
                     /translation="MGRGRVGFELLVGQVLEALPAAPATVVDAGGGTGQLAVALARHG
                     YRVTVVDTSPAMLATCAQRAADEGGEVAGRVATVQGDAADLPGLLGPGALEAAICHDL
                     LTHVEDRAALLASLAGVLADGGVLSLGFANRDWLALRSGRRGDHAAALRLVESGEAMT
                     LAEVEVELDKAGLALVAAYGVGVFAEAGDDDLDQAERRRLVELERLVAGREPYRSSAR
                     TLHLVARRGVFRPDLTGYTP"
BASE COUNT          264 a          839 c          839 g          230 t
ORIGIN      
        1 cgaccatgcg ccggaccacg gcgagctgct gggcgtcctt ctggccgaag taggcgcggc
       61 acggcccggc caggtggagc agcttggcca ccaccgtggc caccccgtcg aggtggccgg
      121 gccggaccag gccctccagc cgctcggcca ggccgccacc gacctggagc cgcacctccg
      181 gcggggccgg ccacatctcc ccctcggccg gcacgaacgc caggtcggtc ccttcggccc
      241 ggaggacggc caggtcggcg tcgcggtcgc gggggtagcc ggccaggtcc tcggagggcc
      301 cgaactgcag cgggttcacg aacaccgacg cgaccacgaa cgcgttctcg gccctggccc
      361 tggccagcag cgaccggtgg ccggcgtgga gggcgcccat ggtgggcacg aagccaaccc
      421 cggcgccgcg ctggcgggcg gcgtcggtgg cggcgcgcag ctcagcgctg ctcgcgacct
      481 cgcgcacgcc gtcccagtct ccggagcagc tccagccgct cgtgggactc ctcgccctcg
      541 acatggccgt gcaggccggc ctcgacccgg gccctggcct ccgggtccag ccgaccggcg
      601 gcggccgccc ggtcggcgac cagccgggcc agggccacgt acacgttgat gaccttctgc
      661 ttgcgggcca gcgcctcggt gtgcatggcc acggtgcggc cgtcgccccg cacccacggc
      721 ccggacaggc cggcctgccc ggtggccagg gccgagtcca cgctggcgtg gatcagcccg
      781 ctgagcaggg ccgccgggtc gatgacgccg ccggcccgca gctggtcggt ggcggtggcc
      841 gagagggcgg cgcagccgtt ggcggccagg acgagggcgg cgtggtagcg gacccggccc
      901 gactcgggca cgtccatggg gcggccgccg agggtggtca cgacctcctt gccggccggg
      961 tccccggtga ccccccagac ggtgccgggc aggcggccga gggtggcctc gaggtcgtcg
     1021 ttgaagatct gcaccgggtg gcaggcggcg gtgcgagcgc cggcggcggc cgccggggcc
     1081 aggaggccgg ggccgtcgac cccggcggtg tggaccacca cctggccggc ggcgaacgcg
     1141 ccgtcggcgg ccagggcggt gaccagcgac ggcagctggt cgtcggggac ggccaggagc
     1201 accagggacg cccctctggt ggccgccgtc gggtcggtca cggccaggcc ggccgcctcg
     1261 acccgctcgc ggccgcggtc ggagcgggcg cccatggtga cctcgacgcc gatccggtcc
     1321 aggaaggtgg ccagggcgag gccgacgcgg cccgggccga tcacggcggc gggcgagagc
     1381 ttgctgctct gggaaggcgg catggaaccc ccggggccgg atgtcacgga gtatatccgg
     1441 tcaagtcagg acgaaatacc ccgcgccggg cgaccaggtg cagggtccgg gcgctcgaac
     1501 ggtagggctc gcgcccggcc accagccgct ccagctcgac cagccgccgc cgctcggcct
     1561 ggtccaggtc gtcgtccccg gcctcggcga acaccccgac cccgtaggcc gccaccaggg
     1621 cgagcccggc cttgtcgagc tccacctcga cctcggccag ggtcatcgcc tcgccggatt
     1681 cgaccagtcg cagggcggcc gcgtggtcgc cgcggcggcc ggaccggagg gccagccagt
     1741 cgcggttggc gaacccgagc gacagcaccc cgccgtcggc caggaccccg gccagggagg
     1801 cgagcagggc cgcccggtcc tcgacgtggg tgagcaggtc gtggcagatg gccgcctcca
     1861 gggcgccggg gccgagcagg ccgggcaggt cggcggcgtc gccctggacg gtggccacgc
     1921 gcccggcgac ctcaccgccc tcgtcggccg cccgctgggc gcaggtggcc agcatggccg
     1981 gcgaggtgtc gaccaccgtg acccggtagc cgtgcctggc cagggcgacg gccagctggc
     2041 cggtgccgcc gccggcgtcg accaccgtcg ccggcgccgc cggcagggcc tccagcacct
     2101 gcccgaccag cagctcgaac ccgactctgc ccctacccac cggggcaccc ccttggggtt
     2161 ccccagaccc ct
//
LOCUS       DASSWR010000396         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1231174, whole genome shotgun sequence.
ACCESSION   DASSWR010000396 DASSWR010000000
VERSION     DASSWR010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231174"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFO47_06565"
     CDS             complement(<1..333)
                     /locus_tag="VFO47_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET8788392.1"
                     /translation="MADGADLGSFPRRHARTQGFTLGRPRGFQVGADGARVAFLRSAA
                     GDDPVNRLWVLDVATGAERLVADPAALLGGGKGGLPPEERARRERARERAGGIVAFAA
                     DLDLNVASF"
     gene            complement(326..781)
                     /locus_tag="VFO47_06570"
     CDS             complement(326..781)
                     /locus_tag="VFO47_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788393.1"
                     /translation="MARKDRERARKRHAIERARRESGWEPTPREERPATKTASTTATS
                     RPAASGRQSPLLKKAQVGDVDRKGRVYERPMLLHPKVALYIYFAVLGVGLVSLLRRDP
                     TLQAVGYGAFAVGMLVIADSKTTWMRALPFIVLAAALAAGAVALAIGLG"
     gene            complement(787..1563)
                     /gene="uppS"
                     /locus_tag="VFO47_06575"
     CDS             complement(787..1563)
                     /gene="uppS"
                     /locus_tag="VFO47_06575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009199473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET8788394.1"
                     /translation="MGPSDLLYRLYERRLAADLRRRGGLPRHIGVILDGNRRWARELG
                     MGAREGYRRGADQIDHLLAWSAELGIPVVTLWLLSTENLLQRHPAELAELLQIIEDKV
                     VQLARDGAPNGYRIVPVGQLEALPERTRATLLAAEEASRGHSGGALHVAVGYGGRTEI
                     TDAVRRLLAEYGDKNLELAEAARLVTPDEIARHLYTAGVPDPDLIIRTSGEVRLSGFL
                     LWQSAHAEYYFADTYWPDFRKVDFLRALRSYAQRRRRFGR"
     gene            1699..1893
                     /locus_tag="VFO47_06580"
     CDS             1699..1893
                     /locus_tag="VFO47_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788395.1"
                     /translation="MAETTEQEGTWTCERCGAVRPASWTCSCGGEGKGERIVPEGWDP
                     ELTRTSRPRLGLIARPDPPE"
     gene            1941..>2669
                     /locus_tag="VFO47_06585"
     CDS             1941..>2669
                     /locus_tag="VFO47_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET8788396.1"
                     /translation="MDRGPWHPATQLEILHAAPAFAVVGVGLAFGLVAMRADPQWDRL
                     ALLAGLLGCNQYAAGALNDAVDAEADAAAGRGKPIQRGVISRRAVATAAVVAGVASLG
                     FGLALGPATFALAVVGLACAWSYDLWLKGTMFSALPFAVAGPIVPLFGYGAAGRFPPV
                     LWWAWPIGALLAVATHLADALPDVERDRATGVRGLATRLGVGRAAAVAAACYAAAVVM
                     ALWSGLAAGDRPVVVAGAALAVVLG"
BASE COUNT          304 a          985 c         1039 g          341 t
ORIGIN      
        1 gaagctggcc acgttgaggt cgaggtcggc ggcgaaggcg acgatgccgc cggcgcgctc
       61 ccgggcccgt tcgcggcggg cccgttcctc gggtgggagg ccgcccttgc cgccgccgag
      121 cagggcggcc gggtcggcga ccagccgctc ggccccggtg gccacgtcca gcacccacag
      181 ccggttgacc gggtcgtccc cggccgccga gcgcaggaac gccacccggg cgccgtcggc
      241 cccgacctgg aacccccgcg gccgcccgag ggtgaacccc tgggtccgcg cgtgccgcct
      301 cggaaaggac cccaggtcgg ccccgtcagc caaggccgat ggccagggcg acggcgccgg
      361 cggccagggc cgcggccagg acgatgaacg gcagggcccg catccaggtc gtcttggagt
      421 cggcgatcac cagcatgccg acggcgaagg cgccgtagcc gaccgcctgc agggtcgggt
      481 cgcgccggag caggctgacc agcccgaccc cgagcacggc gaagtagatg tagagggcga
      541 ccttgggatg cagcagcatc ggccgctcgt acacccggcc cttgcggtcg acgtcgccga
      601 cctgggcctt cttcagcagg ggcgactggc ggccgctggc ggccgggcgg ctggtcgccg
      661 tcgtggacgc cgtcttggtc gccggccgtt cctccctggg cgtcggctcc cagccggact
      721 cccggcgggc ccgttcgatc gcgtgccgct tgcgggcccg ctcccggtcc ttgcgtgcca
      781 ccccgttcac ctcccgaagc gccgccgtcg ctgggcatac gaccgcaggg cgcgaaggaa
      841 gtccacctta cggaagtccg gccagtatgt gtcggcaaag tagtactcgg cgtgggccga
      901 ctgccacagc aggaatccgg acagccggac ctccccggag gtgcggatga tcaggtcggg
      961 gtcgggcacc ccggcggtgt acaggtgccg ggcgatctcg tcgggcgtga ccaggcgggc
     1021 cgcctcggcc agctccaggt tcttgtcgcc gtactcggcc agcagccggc ggacggcgtc
     1081 ggtgatctcg gtccggccgc cgtagccgac ggccacgtgc agggcgccgc cgctgtgacc
     1141 acgggaggcc tcctcggccg ccagcagggt ggcccgggtc cgctcgggca gcgcctccag
     1201 ctggcccacg ggcacgatcc ggtagccgtt gggggcgccg tcgcgggcca gctggaccac
     1261 cttgtcctcg atgatctgga gcagctcggc cagctcggcc gggtggcgct ggagcaggtt
     1321 ctcggtcgac agcagccaca gggtcaccac cgggatgccg agctcggccg accaggccag
     1381 caggtggtcg atctggtcgg cgccgcgccg gtagccctcg cgggcgccca tgcccagctc
     1441 gcgggcccag cggcggttgc cgtccaggat caccccgatg tggcggggca gcccgccccg
     1501 gcggcgcagg tcggcggcca gccgtcgttc gtacaggcgg tagagcaggt ccgatgggcc
     1561 catggggaga tgctacccgc cgccgccatg gggtagcgtc cgcctcgcac accggatatg
     1621 aagatgggac cgaaccgtcg cccggccgtg gcagcggcta ccctgggagc cgacatcggc
     1681 gaacaggaag agggcgcggt ggccgagacg accgagcagg aggggacctg gacgtgcgag
     1741 cgctgcggcg ccgtgcgtcc ggcgagctgg acatgcagct gcggcggcga gggcaagggc
     1801 gagcggatcg tccccgaggg ctgggacccg gagctgaccc ggaccagccg cccccgcctg
     1861 ggcctgatcg cccgccccga cccgccggag tagcgcccgt cggccggggg ggtctggggg
     1921 accgccgggg tggtcccccg gtggatcggg ggccgtggca tcccgcgacc cagctggaga
     1981 tcctgcatgc ggccccggcg ttcgcggtcg tcggggtcgg gttggccttc gggctggtgg
     2041 ccatgcgggc cgacccgcaa tgggaccggc tggccctgct ggccgggctg ctgggatgca
     2101 accagtacgc cgccggggcc ctcaacgacg ccgtcgacgc cgaggccgac gccgccgccg
     2161 gccggggcaa gccgatccag cgcggggtga tctcccgccg ggcggtggcc acggcggccg
     2221 tcgtcgccgg ggtcgcctcg ctcggcttcg gcctcgccct cggcccggcc accttcgccc
     2281 tggccgtggt cgggctggcc tgcgcctggt cctatgacct gtggctgaag gggacgatgt
     2341 tcagcgccct gccgttcgcg gtcgcgggcc cgatcgtgcc gctgttcggc tacggcgccg
     2401 ccgggcgctt cccgcccgtg ctgtggtggg cctggccgat cggcgccctg ctggccgtgg
     2461 ccacccacct ggccgacgcc ctgcccgacg tcgagcggga ccgggccacc ggggtcaggg
     2521 ggctggccac ccggctcggg gtcggccggg cggccgccgt cgccgccgcc tgctacgccg
     2581 ccgccgtggt catggccctg tggtcggggc tggccgccgg ggaccgcccc gtggtggtgg
     2641 ccggggcggc cctggccgtc gtcctcggc
//
LOCUS       DASSWR010000397         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_581104,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000397 DASSWR010000000
VERSION     DASSWR010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581104"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="VFO47_06590"
     CDS             <1..103
                     /locus_tag="VFO47_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005254610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788397.1"
                     /translation="FSDPDGNTWAVQELKIRATRPLIPPEARGRFGG"
     gene            complement(100..1110)
                     /locus_tag="VFO47_06595"
     CDS             complement(100..1110)
                     /locus_tag="VFO47_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004980818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET8788398.1"
                     /translation="MNGFGNAVRRGPMVGLALVLLVGVAACGGSPAPSPPAERAPQVM
                     GTETVGPRVRDLVVDSPALGQYVLVRLLLPRRFEAEPDRRWPVLWLLHGCCDSYESWT
                     RSTDLEALPGLDEVLVVMPDGGQIGFYSDWLDGSTGGPARWETFHLTELRRLLERDWR
                     AGPRRAVAGLSMGGLGAMAYAARHPGMFQAAASYSGLLHTRYDGPPSPGAPIVQNLLG
                     TFGEDPGALWGDPARNAGVWAAHNPHDLAPRLRGVDLFVSAGNGQPGPLDGPAPDAQA
                     QQIEADLYPQSLAFVERLGQLGIPVRFDDYGPGTHDWPYWERELRRSLPLLLDALHRP
                     AG"
     gene            complement(1132..1659)
                     /locus_tag="VFO47_06600"
     CDS             complement(1132..1659)
                     /locus_tag="VFO47_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HET8788399.1"
                     /translation="MQNDQSEWNQVMRDETELQSVDRHFNELLQELRVAQTGVQILFA
                     FLLGLAFTPRFPDLTGEQQTIYLVTLVLSAISAALLIGPVGYHRTVFRQRLRPQLVKT
                     GHRYAIAGLVLLLLALVGAVQLAASFVLGGWASILAAALAGLFATLWFVIPLFHRRRH
                     QRRPDAQDADLPATG"
     gene            1875..>2114
                     /locus_tag="VFO47_06605"
     CDS             1875..>2114
                     /locus_tag="VFO47_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788400.1"
                     /translation="MGREGGTRGGLRFGCRRHRGRRRPPRQPGHRRRQVRRLPGHRGV
                     LGPLRGDQSWRSFIRTKQPELAVVLLEDSAAELGEH"
BASE COUNT          301 a          733 c          812 g          268 t
ORIGIN      
        1 cttctccgat cccgacggca acacctgggc cgtccaggag ctgaagatcc gggccacgag
       61 gccgctgatc ccgccggagg cgcgggggcg gttcgggggc tagcccgccg ggcggtggag
      121 ggcgtccagc agcaggggca gcgaacggcg cagctcccgc tcccagtagg gccagtcgtg
      181 ggtgccgggg ccgtagtcgt cgaaccggac ggggatgccg agctggccca gtcgttccac
      241 gaacgcgagg ctctgcgggt agaggtcggc ctcgatctgc tgggcctgcg cgtcgggtgc
      301 cggcccgtcg agcggcccgg gctggccgtt gccggccgag acgaacaggt cgacgccacg
      361 cagccggggc gcgaggtcgt gggggttgtg ggcggcccag acgccggcgt tccgtgccgg
      421 gtcgccccac agggctcccg ggtcctcgcc aaaggtgccg agcaggttct ggacgatcgg
      481 ggcgcccggc gacggagggc cgtcgtagcg ggtgtgcagc agcccgctgt aggaggccgc
      541 cgcctggaac atccctgggt ggcgtgccgc gtaggccatc gccccgagcc cgcccatgga
      601 gagcccggcc acggcccgtc gcgggccggc ccgccagtcg cgctccagga gccggcgcag
      661 ctcggtcagg tggaaggtct cccagcgggc cggcccgccg gtgctgccgt cgagccagtc
      721 ggagtagaag ccgatctgcc cgccgtcggg catgaccacc agcacctcgt ccaggccggg
      781 aagggcttcg aggtcggtgg agcgggtcca gctctcgtag ctgtcgcagc agccgtgcag
      841 cagccacagc accggccagc gccggtccgg ctcggcctcg aagcggcggg ggagcagcag
      901 ccgcaccagg acgtactggc cgagcgcggg cgagtcgacc accaggtccc gcacccgcgg
      961 cccgaccgtc tcggtgccca tgacctgtgg cgcccgctcc gccggcggcg acggcgccgg
     1021 cgacccgccg caggccgcca cgcccacgag caggaccagc gcgagcccca ccatggggcc
     1081 gcggcgcacg gcgttgccga acccgttcat gcgatcagtg tgcgggacgc ctcaaccagt
     1141 ggcgggcagg tcggcgtcct gggcgtccgg ccggcgctgg tgccggcgcc ggtggaacag
     1201 ggggatcacg aaccacaagg tggcgaacag gccggccagg gcggcggcca ggatcgacgc
     1261 ccaccccccg agcacgaagc tggccgccag ctgcaccgcc ccgaccagcg cgagcagcag
     1321 caggaccaga ccggcgatcg catagcggtg gcccgtcttg accagctggg gccgcagccg
     1381 ctggcggaag acggtgcggt ggtagccgac cgggccgatc agcagcgccg ccgagatcgc
     1441 cgacaggacg agggtgacca ggtagatggt ctgctgctcg ccggtgaggt cggggaagcg
     1501 gggggtgaag gccagcccca ggaggaacgc gaacaggatc tgcacgcccg tctgggcgac
     1561 gcggagctcc tggagcagct cgttgaagtg gcggtcgacc gactgcagct cggtctcgtc
     1621 gcgcatgacc tggttccact cggactggtc gttctgcacc gacgcctggc agtgcgggca
     1681 tcgggctgtc tcgtccgtgg tgttcagtgg tcccggtcgg ccggctgggc gacggagctg
     1741 gtccgtctgc tcggactgcg aacccatcga cgctcccagg gaactcgcca agaatatcca
     1801 tcaggtgtgt accccgattc ccaacccggg taacgccggc ttggtctggt tcgtggaagg
     1861 ggaagggagg acggatgggc agggaaggcg gcacccgcgg cgggctccgg ttcgggtgcc
     1921 ggcgtcaccg cggccgtcgc cgccctcctc gccaacctgg gcatcgccgt cgccaagttc
     1981 gtcggcttcc tggtcaccgg ggcgtcctcg gacctctgcg gggcgaccag agctggcggt
     2041 cgttcatccg gaccaagcaa cccgagctcg ccgtggtgct gctggaggac tcggccgccg
     2101 agctgggcga gcat
//
LOCUS       DASSWR010000398         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_834525,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000398 DASSWR010000000
VERSION     DASSWR010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834525"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..686
                     /locus_tag="VFO47_06610"
     CDS             177..686
                     /locus_tag="VFO47_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8788401.1"
                     /translation="MTATESTATSLEGLLGTVGEAMNAEVVALEADTPVDVALRRLEH
                     TQVSGAPVVDHGRVVGVVTLRDLLGPVLADGPVLTTGPFHRREHQLTTYRVHELMTAE
                     PLTARPDWPLVQAVLAMEQAGINRLPVVDAAGRPAGILTRDDVLRVLARRIRGLGPPA
                     GRGSRIEPD"
     gene            complement(731..949)
                     /locus_tag="VFO47_06615"
     CDS             complement(731..949)
                     /locus_tag="VFO47_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HET8788402.1"
                     /translation="MTRKRWSELSGRQQAAIVAAVVVQEALAAATLWDLRRRPSAQIR
                     GNKRLWVAAAFVNFVGPLAYFTVGRRRG"
     gene            complement(979..1374)
                     /locus_tag="VFO47_06620"
     CDS             complement(979..1374)
                     /locus_tag="VFO47_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788403.1"
                     /translation="MATQLKEDFTEATRWRRRPRPTFAWVAQVISGVLLLVLLTVHMV
                     AQHFVVEGGLRTYADVVAWIRNPVVFAVEALLLVCVTWHGIAGVHSVLLDLGLRGRAE
                     RIVARVLLDLAVATILYGLWLLYMIAFGG"
     gene            complement(1389..1784)
                     /gene="sdhC"
                     /locus_tag="VFO47_06625"
     CDS             complement(1389..1784)
                     /gene="sdhC"
                     /locus_tag="VFO47_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET8788404.1"
                     /translation="MTQVSRHGGNRQRRRPRWLDPRGRNLGTWAFVANRLTGLGLVAY
                     LYLHLVILSTLLRGPEAWDGLVELFRRPVFLAFDLLLVAGLAFHGLNGLRMALVGSGL
                     LVDRHRALLAAAAILVGLVVLVAAVRILA"
     gene            complement(1781..2530)
                     /locus_tag="VFO47_06630"
     CDS             complement(1781..2530)
                     /locus_tag="VFO47_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET8788405.1"
                     /translation="MSAQRTVLLKVFRHGPADAGPRYDEFRVPAGPRATVLDALLAIR
                     RRQDPSLTVRHSCLHGSCGTCGMRVNGREVLACVARLDGLGDPVVVEPLAGVVVIGDL
                     VVDMEHVYRRLEPVGRPLVRASERAGAGDGKAGPGPVLEDLERFEDCIECGLCLSACP
                     VAGDPRFLGPAALAAAERVLEEPRGVDPKAVLGLVDDAHGAWRCHAAFECSAVCPAGV
                     DPGEAIMRLRGRLLRDRLPRLVGRYHREART"
BASE COUNT          397 a          964 c          968 g          307 t
ORIGIN      
        1 aggggcagat cgtggagctg gcaccgggta gcacccgcga gttcccgtgc tgagcgagtg
       61 agggccaccc ggcccggaaa cggagagacg gtcggccgat gcggccgccg cctgaatggc
      121 ggaggctcag cagggaggca tccgtcctcc acaccgccac cgaggaggcg acatcgatga
      181 ccgcaaccga gtccaccgcg accagcctcg agggcctgct cggcacggtc ggcgaggcga
      241 tgaacgccga ggtggtggcg ctcgaggccg acaccccggt cgacgtggcc ctgcgccggc
      301 tggagcacac ccaggtgtcg ggggctcctg tcgtcgacca cggacgcgtg gttggcgtgg
      361 tgaccctgcg cgacctgctc ggccccgtcc tggccgacgg tcccgtcctg accaccggcc
      421 cattccaccg ccgcgagcac cagctgacca cctaccgggt gcacgagctc atgaccgccg
      481 agccgttgac ggcccggccc gactggccgc tggtccaggc ggtcctggcc atggagcagg
      541 ccggcatcaa ccggctgccg gtggtcgacg cggccggtcg gccggccggc atcctgactc
      601 gcgacgacgt gctgcgcgtt ctggcccggc gcatccgggg acttggtccg ccggcaggcc
      661 gcggatctcg gatcgagccg gactaacctg aacccgaggc cggaccgagc tgttgcctcg
      721 gcccgacccg tcaaccccga cggcgaccga cggtgaagta ggccagcggg ccgacgaagt
      781 tgacgaacgc ggccgccacc cacagcctct tgttgccgcg gatctgcgcg ctcgggcggc
      841 gccgcaggtc ccagagggtg gcggcggcca aggcctcctg gaccacgacg gctgcgacga
      901 tcgccgcctg ctggcgcccg ctcagctcgc tccagcgttt ccttgtcatc gtgaacctcc
      961 ttgccggtcc ggttggggtc agccgccgaa ggcgatcatg tacagcagcc agaggccgta
     1021 caggatggtg gccacggcca ggtcgagcag gacccgggcc acgatccgct cggcgcggcc
     1081 gcgcaggccc aggtcgagca ggaccgagtg cacgccggcg atgccgtgcc aggtcacgca
     1141 gaccaacagc agggcctcga cggcgaacac gaccgggttg cggatccagg cgaccacgtc
     1201 ggcgtaggtg cgcaggcccc cctcgaccac gaagtgctgg gcgaccatgt gcacggtgag
     1261 caggaccagc agcagcaccc cggagatgac ctgggccacc caggcgaagg ttggccgggg
     1321 gcggcgccgc cagcgggtcg cctcggtgaa gtcctccttg agctgtgtcg ccatcggttc
     1381 ctcctcgatc aggccaggat gcgtacggcc gcgaccagca cgaccagccc gaccaggatg
     1441 gccgcggccg ccagcagggc ccggtgccgg tcgaccagca gcccggatcc gaccagggcc
     1501 atccgcaggc cgttgagacc gtgaaaggcc aggccggcga ccagcagcag gtcgaaggcc
     1561 aggaacaccg gccggcggaa caactcgacc agcccgtccc aggcctcggg gccccgcagc
     1621 agggtgctga ggatcaccag gtgcaggtac aggtaggcga ccagcccgag cccggtcagc
     1681 cggttggcca caaaggccca ggtgccgagg ttgcgcccgc gcgggtcgag ccagcgcggc
     1741 cgtcgccgtt gccggttccc gccgtggcgg ctgacttggg tcatgtgcgg gcctcccggt
     1801 ggtatcggcc gaccagccgg ggcagccggt cccggagcag gcggccacgc agccgcatga
     1861 tcgcctcgcc cgggtcgacc ccggccgggc agacggccga gcattcgaag gcggcgtggc
     1921 aacgccaggc cccgtgagcg tcgtcgacca ggccgagcac cgccttcggg tccacgccgc
     1981 gtggttcctc caggacccgc tcggccgcgg ccagcgcggc cggacccagg aagcgggggt
     2041 cgccggcgac cggacaggcc gacaggcaca gcccgcactc gatgcagtcc tcgaagcgtt
     2101 ccaggtcctc cagcaccggg cccggacccg ccttcccgtc cccggcgccg gcgcgctcgc
     2161 tggcccgaac cagtggccgg ccgaccggct cgaggcgccg gtagacatgc tccatgtcca
     2221 cgaccaggtc gccgatcacg accaccccgg ccaggggctc caccaccacc gggtcgccca
     2281 ggccgtccag cctggccacg caggccagga cctcgcggcc gttgacccgc atcccgcagg
     2341 tgccgcagga gccgtgcagg caggaatggc ggacggtgag gctggggtcc tgccggcggc
     2401 ggatggccag cagggcgtcg agcacggtgg cgcggggccc ggccggaacc cggaactcgt
     2461 cgtagcgggg gcccgcgtcg gccgggccgt ggcggaagac cttgaggagc acggttcgtt
     2521 gggcgctcat gccgccctcc cggtgtagta ggcggtgtgg ggggccagga cgacctcgcg
     2581 ggtccggcag ggggcgtcga cgccgagctt ggcgcagacc aggtcggcgg ccgcct
//
LOCUS       DASSWR010000399         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_129044,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000399 DASSWR010000000
VERSION     DASSWR010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129044"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFO47_06635"
     CDS             complement(<1..273)
                     /locus_tag="VFO47_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788406.1"
                     /translation="MQPISDREPAGMVVGSHASYRAARQDADSLVSHGFPPDRVSVRV
                     RGLRLVRRRLPTDTSLRRAAGGGLLVGAIGCLLGLWVAGVVPALPAG"
     gene            complement(428..796)
                     /locus_tag="VFO47_06640"
     CDS             complement(428..796)
                     /locus_tag="VFO47_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET8788407.1"
                     /translation="MGKTARAPSWINVAIGMWVLVSPWLLGFTESGGATANAVVTGLA
                     ITMVATLAVARSSPSASWGNLLLAIWLFVSPWVVGYTEFRSASRNAWFIAVMVALFAF
                     MTLITASPERDDRRFPLDTR"
     gene            971..1861
                     /locus_tag="VFO47_06645"
     CDS             971..1861
                     /locus_tag="VFO47_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET8788408.1"
                     /translation="MLIDLLLDNRTVPGKLVSTAAVLALAVLLAVALGRLAAWRVADP
                     YSRYYTRKAVHYLVGLVAIVAVALIWRAFAGRIGVVLGLAAAGVAFAMQEVIGALAGW
                     VNVVSGRIFRVGDRIQMGGVQGDVIDLTPLRTKILEIGSAAEDASWVKGRQHTGRIVA
                     VSNKATFTEPVYNYSSVFEFLWEELTVPIAYRSDWRAAERILHQEAVRASGAEGAEEA
                     MRTMTRRYPVPRAELEPRVFARATDNWMELSARFVVPVRTARSVKDAMTRRVWDRLDA
                     EGIEIASETAEVTVRQPGPG"
     gene            1935..>2914
                     /locus_tag="VFO47_06650"
     CDS             1935..>2914
                     /locus_tag="VFO47_06650"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HET8788409.1"
                     /translation="MAVAIGLAMLLTTIDQRLGQAGSPLVFTGGPEAARTLLSTITSS
                     MITFTGLVFSITVVVLQLTSSQFSPRVLRTFLSDRANQLALGVFTATFVYAMMVLRTV
                     RSSFEGSPFVPQLATTVAVGLVLLSVGIFLYYIHHITNSIRAASIIAGIGRETRAELD
                     RRYPEDGGDPGPPRPASAPARTVPAPRPGVVVLVDERSLVRRAAEVGCVLALVPRVGD
                     FVPAGAPLLEILGRTDGSEDDRLVATIKFGRERTMEQDVAFGFRQLADIADRALSPGV
                     NDPTTALQAIDQLHDLLRRLATRPFPTGRLADDDGRLRLLLPVVGWEEFLA"
BASE COUNT          384 a         1086 c         1024 g          420 t
ORIGIN      
        1 cccggccggg agggcgggca cgacacccgc tacccacagc ccgaggaggc agccgatcgc
       61 ccccaccagc agccccccac ccgcggcgcg ccggagagag gtgtcggtgg ggaggcggcg
      121 tctcaccagc cgcagcccgc ggacccggac cgagacccga tcgggcggga acccgtggga
      181 gacaaggctg tcggcatcct gccgggcggc ccggtagctg gcatgagaac ccaccaccat
      241 cccggctggc tcccgatcgc tgatcggctg catgacgact cctctacacc actggtcgcg
      301 cacttcgtcg gccatgggtg cttgcccgtc gccgccgggc gggaccagtc gtcatccgac
      361 cgcgcggtac gcgaaagctt gccgtagcgc ctggcggccc cggttgatcc ccctgccggg
      421 ggccggttca gcgggtgtcc agcgggaagc gccggtcgtc acgctccggg gacgcggtga
      481 tcagcgtcat gaacgcgaac agggccacca tcacggcgat gaaccaggcg ttgcgggacg
      541 ccgagcggaa ctcggtgtag cccaccaccc acggtgagac gaacagccag atggccagga
      601 gcaggttgcc ccacgatgcg ctcggcgacg acctggccac cgcgagggtg gccaccatgg
      661 tgatggcaag cccggtgacg actgcgttcg ctgtcgcccc gccggactcg gtgaacccga
      721 gcagccaggg gctcaccagc acccacatgc cgatggccac gttgatccag ctcggggcgc
      781 gtgcggtttt gcccatggtc gttgacctcc cttgtctcgg ttgccaggac cagggtcgcc
      841 gcccgcgagc ccacctatcc gcgtcgacaa caggccgtca cctgaccgtt cgccggccgc
      901 aacgacggcg gcggccccga ccgctaccgt ggtcggatgg acgtcgctgg cccgagggcc
      961 gggaggcaac gtgctcatcg acctgctgct cgacaaccgg accgtcccag gcaagctggt
     1021 cagcacggcc gccgtgctgg ccctggccgt gctgctggcg gtggcgctcg ggcggctggc
     1081 cgcctggcgg gtggccgacc cctacagccg ctactacacc cgcaaggccg tgcactacct
     1141 ggtcgggctg gtggcgatcg tggccgtcgc gctcatctgg cgggcgttcg ccggccgcat
     1201 cggcgtggtc ctcggcctgg ccgcggccgg ggtggctttc gccatgcagg aggtcatcgg
     1261 cgccctggcc ggctgggtca acgtcgtctc ggggcgtatc ttccgggtcg gcgaccgcat
     1321 ccagatgggt ggggtgcagg gcgacgtgat cgacctgacg ccgctgcgga ccaagatcct
     1381 ggagatcggg tcggccgccg aggacgcctc gtgggtcaag ggccgccagc acaccggccg
     1441 catcgtggcc gtgtccaaca aggcgacctt caccgagccg gtctacaact actcgtcggt
     1501 gttcgagttc ctctgggagg agctgaccgt gccgatcgcc taccgcagcg actggcgggc
     1561 ggccgagcgg atcctgcacc aggaggcggt gcgggcctcc ggcgccgagg gcgccgagga
     1621 ggcgatgcgg accatgaccc gccgctaccc ggtgccgcgg gccgagctgg agccgcgggt
     1681 gttcgcccgc gccaccgaca actggatgga gctgtcggcc cgcttcgtgg tcccggtgcg
     1741 caccgcccgc tcggtcaagg acgccatgac ccgccgcgtc tgggaccgcc tggacgccga
     1801 gggcatcgag atcgcctccg agaccgccga ggtgacggtg cgccaacccg gtccgggcta
     1861 gggtcgcggt cgtgcgcccc gcccaccttc gagagacggt ccgctccagc ttctggctgg
     1921 tgccggcggc ttgcgtggcg gtcgcgatcg ggctggccat gctgctgacc acgatcgacc
     1981 agcggctggg ccaggccggg tcgccgctgg tgttcaccgg cggcccggag gcggcccgga
     2041 cgctgctgtc caccatcacc tcctcgatga tcaccttcac cggcctggtc ttctccatca
     2101 cggtggtggt cctgcagctc accagcagcc agttctcgcc gcgggtgctg cggacgttcc
     2161 tgagcgaccg ggccaaccag ctcgccctcg gcgtgttcac ggccaccttc gtgtacgcca
     2221 tgatggtgct gcggacggtg cgcagcagct tcgagggctc gccctttgtc ccccagctgg
     2281 ccaccacggt cgccgtcggg ctcgtcctgc tgagtgtcgg catcttcctc tactacatcc
     2341 accacatcac caactcgatc cgggccgcct cgatcatcgc cgggatcggg cgcgagaccc
     2401 gggcggagct ggaccgccgc taccccgagg acggcggcga cccgggcccg ccccggccgg
     2461 cctcggcgcc ggcccgaacc gtcccggcgc ctcgacccgg cgtggtcgtg ctggtggacg
     2521 agcggtccct ggtccggcgg gccgccgagg tcggctgcgt cctggccctg gtcccgcggg
     2581 tcggcgactt cgtccccgcc ggcgccccgc tgcttgagat cctcggccgg accgacggct
     2641 ccgaggacga ccgcctggtc gccaccatca agttcggccg ggagcggacc atggagcagg
     2701 acgtggcctt cggcttccgc cagctcgccg acatcgccga ccgggccctc tcccccggcg
     2761 tcaacgaccc gaccaccgcc ctgcaggcga tcgaccagct ccacgacctg ctgcggcggc
     2821 tcgccacccg gccgttcccc accggccggc tggccgacga cgacggccgg ctgcggctgc
     2881 tgctcccggt ggtcgggtgg gaggagttcc tggc
//
LOCUS       DASSWR010000400         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_14051,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000400 DASSWR010000000
VERSION     DASSWR010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14051"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..353
                     /locus_tag="VFO47_06655"
     CDS             33..353
                     /locus_tag="VFO47_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788410.1"
                     /translation="MAESKANWDQVGEDFKALGRQVKQHYDQRPRPERGAGQEGAAPS
                     ADRRKVDDALEKLREALDQAFSALGDAVRDPQVGAQTKKAAESLGDALTATFAEAGER
                     FRKK"
     gene            complement(340..930)
                     /locus_tag="VFO47_06660"
     CDS             complement(340..930)
                     /locus_tag="VFO47_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HET8788411.1"
                     /translation="MDESPGPRELVALLAEPDRLRALAAVALGAATLPEVAERAELEP
                     RAAARALSRLVAGGLLDGESGKGYRVRTEVLRAAAIPPAPAAGAAPADEPEDAVLRRF
                     VHDGRLLAMPAARGKRLVVLDHLAGLFEPGRRYPEPVVNELLGRYHPDYAMLRRYLVD
                     DGFLAREDVPAPSGSRTVKVYWRTGGTVDLAAPTSS"
     gene            1080..2474
                     /gene="radA"
                     /locus_tag="VFO47_06665"
     CDS             1080..2474
                     /gene="radA"
                     /locus_tag="VFO47_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET8788412.1"
                     /translation="MAKTVRTAYACTECGAASPRWVGRCPACGAWNSLVEEVLERSGP
                     ARATGPVTAPVPITEVAAQAGAHRPTGVDELDRVLGGGLVPGSVVLLAGEPGIGKSTL
                     LLGVLAGLAQDGAVLYVCAEESREQVRLRAERLGALHPGLLLAAETDLGSVRALVERV
                     EPAVVVVDSVQTVADPELAGAPGGVGQVREVAAQLVRLAKERSIATFLVGQVTKEGAV
                     AGPKTLEHLVDVVLNFEGDQHHALRLVRCTKNRFGPAYEVGCFEMTEAGLEPLADPSR
                     LFLSARTAGVPGVACTVSLEGRRPLVTEVQALVVPTGVAVPRRTAQGLDAGRLAILVA
                     VLDRRARIGLAGNDVFAATAGGVRIGEPAADLAVCLALASAARDRQVPSDLITIGEVG
                     LGGEVRPVPQLPRRLAEAARLGFRRALVPATGLPAAARSADIELDPVGSVVESLSVLA
                     PTPTPAPPPIRVVR"
     gene            2471..3538
                     /gene="disA"
                     /locus_tag="VFO47_06670"
     CDS             2471..3538
                     /gene="disA"
                     /locus_tag="VFO47_06670"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HET8788413.1"
                     /translation="MSLDDRLLDVLRQVAPGTALRPGLERILRANTGALIVLGSPPQV
                     EALFTGGFQIDVPFTAQRLSELAKMDGALVLDETAGRILWANVHLMPDPAIATVETGT
                     RHRTAERVARQAGVPVISVSHSMRTITLYVEDWRHVLEDVGSVLSRANQAVATLEHYR
                     ARFDEARTALDVCELHGQATLRDVLTLLGRAELVCRVAGELEQYVAELGSEGRLLRLQ
                     MEELSAGVEDEERLAVRDHLRPRPGWEVAGVLAELRRLTPEELSDLAAVAKAIDLTGD
                     PEALDAPQVPRGHRLLARVPQLPPSVVERVVERFGSLKVILAVGPEDLEEVEGVGAGR
                     ARLLREGLLRLADGARLDGDA"
     gene            3646..4125
                     /locus_tag="VFO47_06675"
     CDS             3646..4125
                     /locus_tag="VFO47_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET8788414.1"
                     /translation="MFKVGDSVIYPQHGAAVIEGLQDREVGGETREYLVLKLTYGDLT
                     LMVPRDNCQEVGIREVCSSEDVEGVLEILRTGESSTQGNWSRRFKGNIERLQSGDIFQ
                     VAEVVRDLTVRDREKGLSAGEKRMLQRSRQILVSELVLACHCDEGKAVLLVDEALES"
     gene            4173..>4419
                     /locus_tag="VFO47_06680"
     CDS             4173..>4419
                     /locus_tag="VFO47_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET8788415.1"
                     /translation="MGVVVPAAGGGLRLGGTGPKALVELAGRALLAHAVEAVEANAST
                     VAVVVVVGHPDALEATGKLIAEEGFAKVTAVVAGGPTR"
BASE COUNT          541 a         1668 c         1641 g          569 t
ORIGIN      
        1 aagctaggac ggagcgaggg aaggagcatg agatggctga gagcaaggcc aactgggacc
       61 aggtcgggga ggacttcaag gccctcggcc gccaggtgaa gcagcactac gaccagcggc
      121 cccgccccga gcgcggcgcc ggccaggagg gcgcggcccc gtcggccgac cgccgcaagg
      181 tcgacgacgc cctcgagaag ctgcgggagg cgctcgacca ggcgttcagc gccctcggcg
      241 acgccgtccg cgacccccag gtgggcgccc agaccaagaa ggccgcggag tcgctgggcg
      301 acgccctgac cgccaccttc gccgaggccg gcgagcggtt caggaagaag taggcgcggc
      361 caggtcgacc gtcccgccgg tccgccagta gaccttgacc gtcctcgacc cggagggcgc
      421 cggcacgtcc tcccgggcga ggaacccgtc gtcgaccagg tagcgccgga gcatggcgta
      481 gtcggggtgg tagcggccga gcagctcgtt caccaccggc tcggggtagc gccgcccggg
      541 ctcgaacagc ccggccaggt ggtcgagcac gaccagccgc ttgccccgcg ccgccggcat
      601 ggccagcaga cggccgtcgt ggacgaagcg gcgcagcacc gcgtcctccg gctcgtcggc
      661 cggggcggcg ccggccgccg gggccggcgg gatggccgcc gcccggagga cctcggtccg
      721 gacccggtac cccttgccgg actcgccgtc gagcagcccg ccggccacca gccggctgag
      781 ggcccgggcg gcggcccggg gctccagctc ggcccgttcg gccacctcgg gcagggtcgc
      841 ggcgccgagc gcgaccgcgg ccagggcccg gagccggtcc ggctcggcca gcagggccac
      901 cagctcacgt ggtccgggcg actcgtccat gtccccattg tggacgacat tcgattgtcg
      961 tcggcgcgtg ctagcatttc gcctggtgag ggcgcctgtc ggcgtcctgc cggcgggcat
     1021 cgggcgcgcc ggtccgggcc gggtggcccg gcccgatggt gggggcgagc gcatccagca
     1081 tggccaagac agtccggacc gcctatgcct gcaccgagtg cggggccgcc agcccgcgct
     1141 gggtcggccg ctgcccggcc tgcggcgcct ggaacagcct ggtcgaggag gtgctggagc
     1201 gctccggccc ggccagggcg accggcccgg tgaccgcccc cgtccccatc accgaggtcg
     1261 ccgcccaggc gggcgcccac cggcccaccg gcgtcgacga gctcgaccgg gtcctcggcg
     1321 gcggcctggt gcccgggtcg gtggtgctgc tggccggcga gccgggcatc ggcaagtcga
     1381 ccctcctgct cggggtcctg gccggcctcg cccaggacgg cgcggtgctg tacgtctgcg
     1441 ccgaggagtc gcgcgagcag gtgcggctgc gggccgagcg cctcggggcc ctgcacccgg
     1501 ggctcctgct ggccgccgag accgacctcg ggtccgtccg cgccctggtc gagcgggtcg
     1561 agccggccgt ggtcgtggtc gactcggtcc agaccgtggc cgacccggag ctggccggcg
     1621 cccccggcgg ggtcggccag gtccgcgagg tcgccgccca gctggtgcgg ctggccaagg
     1681 agcgctcgat cgccaccttc ctggtcggcc aggtcaccaa ggagggcgcc gtcgccggcc
     1741 ccaagaccct tgagcacctg gtcgacgtgg tcctcaactt cgagggcgac cagcaccacg
     1801 ccctgcgcct ggtccgctgc accaagaacc gcttcggccc cgcctacgag gtcggctgct
     1861 tcgagatgac cgaggccggc ctggagccgc tggccgaccc ctcgcggctg ttcctgtcgg
     1921 cccgcaccgc cggggtgccc ggggtcgcct gcacggtcag cctggagggc cgccggcccc
     1981 tcgtcaccga ggtccaggcg ctggtcgtgc ccaccggggt ggccgtgccc cggcgcaccg
     2041 cccaggggct ggacgccggc cggctggcca tcctggtcgc cgtgctcgac cgccgggccc
     2101 ggatcggcct ggccggcaac gacgtgttcg ccgccaccgc cggcggggtc aggatcggcg
     2161 agcccgccgc cgacctggcc gtgtgcctgg ccctggcctc ggcggcccgc gaccggcagg
     2221 tgccgtccga cctgatcacc atcggtgagg tcggcctggg cggcgaggtc cgcccggtgc
     2281 cgcagctgcc gcgccgcctg gccgaggcgg cccggctcgg cttccgccgc gccctcgtcc
     2341 ccgccaccgg cctgcccgcc gcggccaggt ccgccgatat cgagctcgac ccggtcggct
     2401 ccgtggtcga gtccctgtcg gtcctggcgc cgacccccac cccggccccg cccccaatcc
     2461 gggtggtgcg atgagtctcg acgaccgcct gctcgacgtg ctgcggcagg tcgccccggg
     2521 gacggccctg cggcccgggc tcgagcggat cctgcgcgcc aacaccggtg ccctgatcgt
     2581 gctcggctcg cccccgcagg tggaggcgct gttcaccggc ggcttccaga tcgacgtgcc
     2641 gttcaccgcc cagcgcctct cggagctggc caagatggac ggcgccctgg tcctggacga
     2701 gacggccggc cgcatcctct gggccaacgt ccacctgatg cccgacccgg ccatcgccac
     2761 cgtcgagacc ggcacccgcc accgcaccgc cgagcgggtg gccaggcagg ccggggtgcc
     2821 ggtgatctcg gtctcccact ccatgcgcac catcaccctg tacgtcgagg actggcgtca
     2881 cgtgctcgag gacgtcggct cggtgctgtc ccgggccaac caggcggtcg ccaccctgga
     2941 gcactaccgg gcccgcttcg acgaggccag gaccgccctg gacgtgtgcg agctgcacgg
     3001 ccaggccacc ctcagggacg tcctgaccct gctcggccgg gccgagctgg tctgccgggt
     3061 ggccggcgag ctggagcagt acgtggccga gctgggcagc gagggccgcc tgctgcggct
     3121 gcagatggag gagctgagcg ccggcgtgga ggacgaggag cggctggccg tccgcgacca
     3181 cctgcggccg cgtcccggct gggaggtggc gggggtgctg gccgagctgc gccgcctgac
     3241 ccccgaggag ctgtcggacc tggccgccgt ggccaaggcg atcgatctca ccggcgaccc
     3301 cgaggccctc gacgcccccc aggtcccgcg cggccaccgc ctgctggcca gggtgccgca
     3361 gctgccgccg tcggtggtcg aacgggtggt cgagcgcttc ggctcgctca aggtgatcct
     3421 ggccgtcggc ccggaggacc tggaggaggt cgaaggggtc ggcgccgggc gggcccgcct
     3481 cctccgcgag gggctcctgc gcctcgccga cggcgcccgc ctcgacggcg acgcatgacc
     3541 ctcacctacg tcaatcaggc cgtctacctg cgacttcgtt tgtacgtagg ccctcggaaa
     3601 tgttatcctg tttagtgttc cgttcgaggg aaggggtttt cgcgtatgtt caaggtcggc
     3661 gacagcgtca tctacccaca gcatggggcc gcggtcatcg agggcctcca ggatcgtgag
     3721 gtaggcggcg agaccaggga gtacctggtc ctcaagctga catatggcga cctgaccctc
     3781 atggtgcccc gcgacaactg ccaggaggtc gggattcgcg aggtctgcag ctccgaggac
     3841 gtcgaaggcg tcctcgagat cctgcgcact ggcgagtcct cgacccaggg caactggagc
     3901 cgccgcttca agggcaacat cgagcggctc cagtccggcg acatcttcca ggtcgccgag
     3961 gtcgtccgcg acctcaccgt gcgcgaccgc gagaagggcc tctccgccgg cgagaagcgc
     4021 atgctccagc gctcccgcca gatcctcgtc tccgagctgg tgctcgcctg ccactgcgac
     4081 gagggcaagg ccgtcctgct ggtcgacgag gcgctcgagt cgtagccagc ccggacgctt
     4141 ccgtcacgcc caccgccgcc gcggcggtgg cggtgggcgt ggttgtgccg gccgccggcg
     4201 gcggcctgcg cctcggcggc accggcccca aggccctggt cgagctggcc gggcgggccc
     4261 tgctggccca cgccgtcgag gcggtcgagg ccaacgccag cacggtcgcg gtggtggtgg
     4321 tggtgggcca cccggacgcc ctggaggcca ccggcaagct gatcgccgag gagggcttcg
     4381 ccaaggtgac cgccgtcgtc gccggcgggc cgacccgcc
//
LOCUS       DASSWR010000401         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_298283,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000401 DASSWR010000000
VERSION     DASSWR010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298283"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1006
                     /locus_tag="VFO47_06685"
     CDS             107..1006
                     /locus_tag="VFO47_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003594185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8788416.1"
                     /translation="MAVRGVAVGLVGAGPWAEMLHAPMLAGGPETSLAGVWARRPAAA
                     RALAEAHGSRAVDSLEELFDLCEAVAFAVPPDIQARIATDAARAGRHLLLDKPVAVTV
                     AAAEELAAAVGEAGVVSQMVLTNRYGPRGRAFLAEAEGFEVVGARCASLSGALLDESP
                     FATPWRRRHGALLDVGPHLFDLVEAAVGPVEELSGRGDPLRWVSLTCRHAGGEVSEIS
                     MSLTLPLVPTVFECSLYGPGGALTYRRPPDPPAAMADLQAAGATLRREFAAAVTSGNA
                     SALDVNRGLRLQRLIDAAARSLG"
     gene            1150..1857
                     /locus_tag="VFO47_06690"
     CDS             1150..1857
                     /locus_tag="VFO47_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788417.1"
                     /translation="MSAPTLDRHPSSSQSGPSAGQRRRRKQLVLGASALIVLAVAVGT
                     SLAVATRPVDRQAAQAGPAPAAAAGDQNANAGQDPAAQPDQNAPAPPAAEQGSGADPD
                     PAGTDGTAPALEDGEHPAYITKVSSGQLVVDVVQVFHDDAAVKAAIADGKPPADARYL
                     TTWVRNENSRLRTLPLAGDLDIRLRDACGDPGDDRQALLTKLAANAKLKGTYYYLLTV
                     DDGKVERIQERLAVNAC"
     gene            1901..>2450
                     /locus_tag="VFO47_06695"
     CDS             1901..>2450
                     /locus_tag="VFO47_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788418.1"
                     /translation="MTPAPSALVGRDQPLAELRALVEAALASRGGVVTLTGEAGIGKT
                     TLATAAADHAEARGATVVWGTCWEGAPPFWPWLEVLAALGAEVDLQADRQGGDRLRPP
                     SAAAARFERFAAISGALLRAAGSLVVVLDDLHWADPASVELAAFHARRARRSRQLLVA
                     TYRDVEIAPGDPLGRPIARLEAE"
BASE COUNT          294 a          904 c          961 g          291 t
ORIGIN      
        1 agcttggaag gcttgtgctc tgccattgag ctacacccgc gcagtgctcg ggtgagggta
       61 tcagggcggg tcgtgagaga ttggcggaaa gcgagcgggg aggctggtgg ctgtgcgcgg
      121 agttgccgtg gggctggtcg gggcggggcc gtgggcggag atgctgcatg cgccgatgct
      181 ggccggggga ccggagacgt cgctggccgg ggtgtgggcg cggcggccgg cggcggcgcg
      241 ggcactggcc gaggcccacg ggagccgggc cgtggacagt ctggaggagc tcttcgacct
      301 ctgtgaggcg gtggcgttcg cggtgccgcc ggatattcag gcgcggatcg cgaccgacgc
      361 ggccagggcc gggaggcacc tgctgctcga caagccggtg gccgtgacgg tcgcggcggc
      421 cgaggagctg gccgcggccg tcggcgaggc cggggtggtg tcccagatgg tgctgaccaa
      481 ccggtacggg ccccgggggc gggcgttcct ggccgaggcc gaggggttcg aggtcgtggg
      541 ggcgcgctgt gcctcgctgt cgggggcgct gctggacgag agcccgttcg cgaccccgtg
      601 gcggcgccgg cacggggcgc tgctcgacgt cgggccgcac ctgttcgacc tggtggaggc
      661 ggccgtcggg ccggtcgagg agctgtcggg gcggggcgac ccgctgcggt gggtgtcgct
      721 gacctgccgg cacgccggcg gcgaggtcag cgagatctcg atgtcgctca cgctgccgct
      781 ggtcccgacc gtgttcgagt gctcgctcta cggcccgggt ggggcgctga cctaccggcg
      841 gccgcccgac ccgccggcgg ccatggccga cctccaggcc gccggggcga ccctccggcg
      901 ggagttcgcc gccgccgtga ccagcgggaa cgcctcggcc cttgacgtca accgggggtt
      961 gcggctgcag cggctgatcg acgccgccgc ccgctcgctc ggctgagaca ctccccggac
     1021 cggtcgatgt ggccgaacgg tgacacgacc gagagcgaag cgtaccgagc aagccaccgc
     1081 ttcgtgaccc gccggaccca ggctggggtg cagcgccgca cggacgcggc gcgggagaga
     1141 ggagccgtca tgtcggcacc aaccctggac aggcacccca gcagctcgca gtccgggccg
     1201 tccgcgggcc agcgccgccg tcgcaagcag ctcgtgctgg gggcgagcgc cctgatcgtg
     1261 ctggccgtcg ccgtcggcac cagcctggcc gtggccaccc ggccggtcga ccgccaggcc
     1321 gcccaggccg gccccgcccc cgcggcggcc gccggcgacc agaacgccaa cgccggccag
     1381 gacccggccg cccagcccga ccagaacgcc ccggccccgc cggccgccga gcaggggtcg
     1441 ggcgccgatc ccgacccggc cggcaccgac gggacggcgc ccgccctgga ggacggcgag
     1501 caccccgcct acatcaccaa ggtctccagt ggccagctcg tggtcgacgt ggtccaggtg
     1561 ttccacgacg acgccgccgt caaggcggcc atcgccgacg gcaagccgcc cgccgacgcc
     1621 aggtacctga ccacctgggt ccgcaacgag aactcgcggc tgcgcaccct gcccctggcc
     1681 ggcgacctgg acatccggct gcgggacgcc tgcggcgacc ccggcgacga ccgccaggcc
     1741 ctgctgacca agctggccgc caacgccaag ctgaagggca cctactacta cctgctgacc
     1801 gtggacgacg ggaaggtcga gcggatccag gagcggctgg ccgtgaacgc ctgctgacac
     1861 ccttgcgcga gggggtcggg gagcgcaccg ttctggtgcg atgaccccgg ccccctccgc
     1921 gcttgtcggc cgcgaccagc cgctcgccga gctgcgagcg ctggtcgagg ccgcgctggc
     1981 gtcccggggc ggggtcgtga ccctcaccgg cgaggccggg atcggcaaga ccaccctggc
     2041 cacggccgcc gccgaccacg ccgaggcgcg gggcgcgacc gtcgtctggg gcacctgctg
     2101 ggagggcgcc ccgccgttct ggccctggct ggaggtcctg gccgcgctcg gggccgaggt
     2161 cgacctgcag gccgaccgcc agggcggcga ccggctgcgc cccccgtcgg ccgcggccgc
     2221 ccgcttcgag cgcttcgccg ccatcagcgg cgccctgctt cgagcggccg ggtcgctggt
     2281 ggtcgtgctc gacgacctgc actgggccga cccggcgtcg gtcgagctgg ccgccttcca
     2341 cgcccgccgg gcccgccgca gccgccagct gctggtcgcg acctaccgcg acgtcgagat
     2401 cgctcccggc gacccgctcg gccgcccgat cgcccggctg gaggcggagg
//
LOCUS       DASSWR010000402         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_749757,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000402 DASSWR010000000
VERSION     DASSWR010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749757"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFO47_06700"
     CDS             complement(<1..594)
                     /locus_tag="VFO47_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788419.1"
                     /translation="MSEPQAAVTVRGLVKRYKSVEAVRGIDLDVAPGETFGFLGPNGA
                     GKSTTIKILCTLVDATAGSATVAGFDVARQRNQVRRHIGLVFQEPTLDTYLTAEQNLR
                     FHGELYGVDRATAASRRREVLTMVGLWERRGDLVQTFSGGMRRRLEIARGLLHSPRVL
                     FLDEPTIGLDPKTRASIWEYIAELHRREQITVFVTTHY"
     gene            646..990
                     /locus_tag="VFO47_06705"
     CDS             646..990
                     /locus_tag="VFO47_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET8788420.1"
                     /translation="MVRGAVDLLARASLEALTLRRLTTELGVSAPTLYWHVRNERQLL
                     DLMAEALRTVLAVGDYVIGCAVEYQAEAAREPDPAASFEHGLGLLIAGIRARHAELVG
                     TTAAAGTPVEAG"
     regulatory      1009..1234
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1263..3572
                     /gene="metE"
                     /locus_tag="VFO47_06710"
     CDS             1263..3572
                     /gene="metE"
                     /locus_tag="VFO47_06710"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET8788421.1"
                     /translation="MTSSTVHGFPRIGDRRELKGATEGFWAGRVPAEELEATARDLRR
                     QTWTFLRDAGLGHVPSNHFSLYDQVLDTCVLVGAVPGRHRRAEGLDAYFAMARGRDDV
                     TAMEMTKWFDTNYHYLVPELGPDAEFRLAAAPKPLAELAEARALGIATRPVLLGPVTF
                     LLLGKAAAGVPAGFDRLSLLDPLLEVYAELLEELEAAGAEWVQLDEPAFVQDRSAAEL
                     DALGHAYDRLGGSSARPRLLVSSYFDHLGDALGVLAGAPVDGIGLDLTRDGVRNLDLL
                     AGAGGIGDRTLVAGVVDGRNVWVNDLERSLSVLATLLGLAGEVVVSTSCSLQHVPIDL
                     EAEQALDPEVRPWLAFARQKVAEVVTLARGLAEGTGAIAGELEANRRTLAARRRSGRT
                     TDPAVRRRLDAIGEPDLHRASPYDVREKAQRARLGLPPLPTTTIGSFPQTGEVRKARA
                     ARRRGELDEAGYQARMRAEIDEVVRIQDELELDVLVHGEPERNDMVQYFGEQLDGVAV
                     TELGWVQSYGTRYVRPPIIFGDVSRPAPMTVEWLTYAQSLTERPVKGMLTGPVTMLKW
                     SFERDDQPREATARQLALAIRDELADLEAAGIGVVQVDEPALREALPLRRDRRDAYLT
                     WAVEAFRLVTAGVRDETQVHTHMCYSEFGEILPAIDGLDADVTSIEAARSRMELVRDL
                     EQAGYGREIGLGVYDIHSPRVPPPDEMASLLEEALEAVGPARLWVNPDCGLKTRDYPE
                     VLASLRNMVAAARRVRATTARSRPPADDR"
     gene            complement(3600..4220)
                     /locus_tag="VFO47_06715"
     CDS             complement(3600..4220)
                     /locus_tag="VFO47_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HET8788422.1"
                     /translation="MSQYGTAPPAGSLLAQQVLVVRQKLKLVELRNEYAVLDPGGRQI
                     GAVFQATQSPLAFLARLFTSWDVTLPITLHILGPGAQLELVVHKPWFTWRCQVLRPDG
                     MPLGEIAKQIRLGRARFTLLDPRGAQLGEVRAHNWRAKDFSVFDGAGQAIARVTKRWA
                     GLRELFTDADTYVVEVSPSAVDPLRSLAVATCLVIDVVMKQKDYAD"
BASE COUNT          621 a         1667 c         1651 g          598 t
ORIGIN      
        1 gtagtgggtg gtgacgaaca ccgtgatctg ctcgcggcgg tgcagctcgg cgatgtactc
       61 ccagatcgac gcccgggtct tggggtccag cccgatggtc ggctcgtcca ggaacagcac
      121 ccgcggcgag tgcagcaggc cccgagcgat ctccagccgc cgccgcatgc cgcccgagaa
      181 ggtctggacg agatcgcccc gccgctccca caggccgacc atggtcagca cctcgcgccg
      241 gcggctggcc gcggtggccc ggtcgacgcc gtacagctcc ccgtggaacc tgaggttctg
      301 ctcggccgtc aggtaggtgt cgagggtcgg ctcctggaag acgaggccga tgtggcggcg
      361 gacctggttg cgctggcggg cgacgtcgaa gccggccacc gtggccgacc cggccgtggc
      421 gtcgaccagc gtgcacagga tcttgatggt ggtcgacttg ccggccccgt tgggcccgag
      481 gaagccgaag gtctccccgg gagccacgtc caggtcgatg ccgcgcaccg cctcgaccga
      541 cttgtagcgc ttgaccaggc cacggaccgt gaccgccgcc tgcggctcgc tcatggcacc
      601 tccgctcccg ccattctcca acaacgttca agatgctacg agcagatcgt ccgcggggcc
      661 gtcgacctgc tggccagggc gagcctggag gccctgaccc tgcggcggct aaccaccgag
      721 ctcggggtgt cggcgccgac cctctactgg catgtccgca acgagcgcca gctgctcgac
      781 ctcatggccg aggccctgcg cacggtcctg gccgtcggcg actacgtgat cggctgcgcc
      841 gtggagtacc aggccgaggc ggcccgcgag cccgaccccg cggccagctt cgagcacggg
      901 ctgggcctgc tgatcgccgg catccgcgcc cggcatgccg agctggtcgg gaccacggcc
      961 gccgccggca cccccgtcga ggcgggttga caggcgccgt gggcgggacg atccttgccg
     1021 ggcgctggtc gccaaagagg gaatccggtg cgactccgga gctgccccgc agcggtatgt
     1081 gggaacgaca gccgtcaccg aggtgccacc cgctcctcgg tccagcactg agccagacag
     1141 tcttgggaag cgacggccgg taggaacccg gcgcggcgaa gaccgcgccg cccgcccacg
     1201 agcccgaaga cctgccagcg cccgcgcggg tgcgcggccg tccaggcctc gagggagggc
     1261 tcatgacaag ttcgaccgtt cacgggttcc cgcggatcgg tgaccgccgg gagctgaagg
     1321 gtgccaccga gggcttctgg gccggccggg tgccggccga ggagctggag gcgacggcca
     1381 gggacctgcg ccgccagacc tggacgttcc tgcgcgacgc cggcctcggg cacgtcccct
     1441 cgaaccactt ctcgctgtac gaccaggtgc tcgacacctg cgtcctggtc ggcgccgttc
     1501 ccgggcgcca ccgccgggcc gagggccttg acgcctactt cgccatggcc agggggcggg
     1561 acgacgtcac cgccatggag atgaccaagt ggttcgacac caactaccac tacctggtcc
     1621 cggagctggg gccggacgcc gagttccggc tggcggccgc gcccaagccc ctggccgagc
     1681 tggccgaggc gcgcgcgctg ggcatcgcca cccgcccggt cctgctcggc cccgtcacct
     1741 tcctgctgct gggcaaggcc gccgccggcg tcccggccgg gttcgaccgc ctcagcctgc
     1801 tcgatccgct gctggaggtg tacgccgagc tgctggagga gctggaggcc gccggggccg
     1861 agtgggtgca gctggacgag ccggccttcg tccaggaccg ctcggccgcc gagctcgacg
     1921 ccctcggcca tgcctacgac cgcctcggcg ggtcgagcgc ccggccccgg ctgctggtct
     1981 ccagctactt cgaccacctg ggcgacgccc tcggggtgct cgccggcgcc ccggtcgacg
     2041 gcatcggcct cgacctgacc cgggacgggg tccgcaacct cgacctgctg gccggggccg
     2101 gcggcatcgg cgaccggacc ctggtcgccg gggtggtcga cggccgcaac gtctgggtga
     2161 acgacctgga gcggtcgctc tccgtcctgg ccaccctgct cggcctggcc ggcgaggtgg
     2221 tcgtctccac ctcctgctcg ctccagcacg tccccatcga cctggaggcc gagcaggcgc
     2281 tcgacccgga ggtccggcca tggctggcct tcgcccgcca gaaggtcgcc gaggtggtca
     2341 ccctggcccg cgggctggcc gagggcaccg gggccatcgc cggggagctg gaggcgaacc
     2401 gccggaccct ggccgcccgg cggcgctcgg gccggaccac cgacccggcc gtccgccgcc
     2461 ggctggacgc gatcggcgag cccgacctgc accgggccag cccgtacgac gtccgcgaga
     2521 aggcccagcg ggcccggctc ggcctgccgc ccctgccgac gaccaccatc ggctcgttcc
     2581 cccagaccgg cgaggtccgc aaggcccggg cggcccgccg ccggggcgag ctggacgagg
     2641 ccggctacca ggcccgcatg cgggccgaga tcgacgaggt cgtccgcatc caggacgagc
     2701 tggagctgga cgtgctcgtc cacggggagc ccgagcgcaa cgacatggtc cagtacttcg
     2761 gggagcagct ggacggggtg gccgtgaccg agctcggctg ggtccagtcc tacggcaccc
     2821 gctacgtccg cccgccgatc atcttcggcg acgtgtcccg cccggccccg atgaccgtcg
     2881 agtggctcac ctacgcccag tccctgaccg agcggccggt caagggcatg ctgaccgggc
     2941 cggtgacgat gctgaagtgg tcgttcgagc gcgacgacca gccacgggag gcgaccgccc
     3001 gccagctcgc cctggccatc cgcgacgagc tggccgacct ggaggcggcc gggatcgggg
     3061 tcgtccaggt cgacgagccg gccctgcgcg aggccctgcc cctgcgccgc gaccgccgcg
     3121 acgcctacct gacctgggcg gtggaggcgt tccggctggt caccgccggg gtccgcgacg
     3181 agacccaggt ccacacccac atgtgctact cggagttcgg cgagatcctg cccgcgatcg
     3241 acgggctgga cgcggacgtg acctcgatcg aggccgcccg ttcgcgcatg gagctggtcc
     3301 gggacctgga gcaggccggc tacgggcgcg agatcggcct cggggtctac gacatccact
     3361 cgccccgggt gccgcctccc gacgagatgg ccagcctgct cgaggaggcg ctggaggcgg
     3421 tcggcccggc ccggctctgg gtcaaccccg actgcggcct caagacccgc gactacccgg
     3481 aggtgctggc ctccctgcgc aacatggtgg cggcggccag gagggtccgg gcgacaacgg
     3541 ctcgaagtcg gcctccggcc gacgatcggt agattccgac cggcgaacct gccggtcggc
     3601 tagtcggcgt agtccttctg cttcatgacc acgtcgatga ccaggcaggt ggccacggcc
     3661 aggctgcgca gggggtcgac ggcggacggg ctgacctcga cgacgtaggt gtcggcgtcg
     3721 gtgaacagct cgcgcaggcc ggcccagcgc ttggtcacgc gggcgatcgc ctggccggcg
     3781 ccgtcgaaga cggagaagtc cttggcccgc cagttgtggg cccggacctc gccgagctgg
     3841 gcgccgcgcg ggtcgagcag ggtgaagcgc gccctgccga ggcggatctg cttggcgatc
     3901 tcgcccagcg gcatgccgtc gggccgcagc acctggcagc gccaggtgaa ccacggcttg
     3961 tgcaccacca gctcgagctg ggcgcccggg ccgaggatgt gcagggtgat cggcagggtg
     4021 acgtcccagc tggtgaacag ccgggccagg aaggccagcg gcgactgggt ggcctggaac
     4081 acggcgccga tctggcggcc gcccgggtcg agcacggcgt actcgttgcg cagctcgacc
     4141 agcttgagct tctgcctgac caccagcacc tgctgggcga gcagcgaccc cgccggcggc
     4201 gccgtcccgt actggctcat gctcccaggc tagcccttgg tggccttgag cgagtaccgc
     4261 accgggagcc gctggtcata cgcctcgggc agccgccaga cccatgctgt gctgccaggc
     4321 gtactcgaac ggctcgctca cgggggcgcc cgggtcggcg taggacccct cgtggccgtg
     4381 tcgtcgtcgt cgaagaccga ggcgaacggg tgggcctcgg cgatgtagaa gaagccgccg
     4441 gggggcccgg tggggcgagg ccatagtcag gagtccgaac ccggatgagc gcaacgctcc
     4501 ccggctgaag ggcaaatccc ccgctcgccc cgcccca
//
LOCUS       DASSWR010000403         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_779013,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000403 DASSWR010000000
VERSION     DASSWR010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779013"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="VFO47_06720"
     CDS             complement(<1..1962)
                     /locus_tag="VFO47_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8788423.1"
                     /translation="MARDGEQVRAGPAGAGQDGDGPRRRPLRDRLQLWALVELFVLCG
                     FAIAQPLLDVTGRSPEFFLFRRADRLDIVALALGLLLLPALGIWTAEVLTGLVNERAR
                     RYLHLAAVAGLVTLVGIQVAKKLTGLRGPLVVAIALAVGALAAVVYARASWPHLWLRY
                     LAPAPLVFALVFLLLSPSSRLVLPARAEASAAAAPAAAAGRQPPRVMILFDEFPLSSL
                     LDHTGRIDRRVYPNLAALADQSTWYRNATGVAGFTPWALPAMLTGRYPDRTRAPSYTE
                     YPDNMLTLFGRYYDLKVYETISQLCPPSRCRFSDGHLDQAGLGAVLAETARSYRKLIS
                     PYDAAEDPASFADQTEAEAGRPLRPTFRFDRMRLNQPSRFNDFLDGLRASDRPTMHVL
                     HLLLPHPPWRYLPSGAEYNDKSFAVAHKTEEAPAPVVQLAHQRHLLQVAYTDRLLGQV
                     MAKLQAEGLWDKSLVVLGADHGEGWVPGEKPRSLGETNAPDLMWVPQFIKAPGQDNGR
                     VDDRNWEQVDLLPTIADLVSIQVPWKTEGTSQAGRPTRTRTQKWWFDIPGHREVRDGP
                     ANWRVVLAGETDTLVRGSEGIRGLYRYGPLADLVYRDPAAVGPIDGDPATAVLDDGRL
                     FETIEPGSGDVPALVSGRLASPPAGATILV"
BASE COUNT          302 a          735 c          815 g          277 t
ORIGIN      
        1 gaccaggatg gtggcgccgg ccggcggcga ggcgagcctg cccgagacca gggccggcac
       61 gtctcccgag ccgggctcga tcgtctcgaa caaccggccg tcgtccagca cggcggtagc
      121 cgggtcgccg tcaatggggc cgaccgcagc cgggtcccgg tagaccaggt cggcgagggg
      181 accgtagcgg tacaggccgc ggatgccctc cgagccccgg accagggtgt cggtctcgcc
      241 ggcgagcacg accctccagt tggcgggccc gtcgcggacc tcgcggtgcc cagggatgtc
      301 gaaccaccac ttctgggtcc gggtccgggt cggtcgacca gcctgggagg tgccctccgt
      361 cttccagggc acctggatgc tcacgaggtc ggcgatggtg ggcagcaggt cgacctgctc
      421 ccagttgcgg tcgtccacgc ggccgttgtc ctggccgggg gccttgatga actgcggcac
      481 ccacatcagg tcgggggcgt tggtctcgcc caggctgcgc ggcttctcgc ccggcaccca
      541 gccctcgccg tggtcggccc ccagcaccac cagcgacttg tcccacaggc cttcggcctg
      601 gagcttggcc atcacctgcc cgagcaaccg gtcggtgtag gccacctgga gcaggtgccg
      661 ctggtgggcc agctggacca ccggagcggg ggcctcctcg gtcttgtggg cgacggcgaa
      721 ggacttgtcg ttgtattcgg cgccggaggg caggtagcgc cagggcgggt gcggcagcag
      781 caggtgcagg acatgcatgg tcggccggtc gctggccctg aggccgtcga ggaagtcgtt
      841 gaagcgcgac ggctggttga gccgcatccg gtcgaaccgg aacgtcgggc gaagcggccg
      901 gcccgcctcg gcctcggtct ggtcggcgaa ggaggctggg tcctcggcgg cgtcgtaggg
      961 cgatatgagc ttccggtagg accgggccgt ctcggccagc accgccccca gccccgcctg
     1021 gtcgaggtgg ccgtcgctga agcggcagcg gctgggcggg cagagctggc tgatggtctc
     1081 gtacaccttg aggtcgtagt agcggccgaa cagggtcagc atgttgtcgg ggtactcggt
     1141 gtaggacggg gccctggtcc ggtccgggta cctgccggtc agcatcgccg gcagcgccca
     1201 cggcgtgaac ccggccaccc cggtggcgtt gcggtaccag gtggactggt cggccagggc
     1261 ggcgaggttc gggtagaccc gccggtcgat ccggccggtg tggtcgagca gggagctgag
     1321 ggggaactcg tcgaacagga tcatgacccg cgggggctgg cggccggcgg cggccgccgg
     1381 ggcggctgcg gcgctggcct cggcccgggc cggcaggacc agcctggagg agggggacag
     1441 cagcaggaag accagggcga acaccagcgg cgcgggcgcc aggtagcgca gccacaggtg
     1501 cggccacgac gccctggcgt acaccaccgc ggccagggcg cccacggcca gggcgatcgc
     1561 caccaccaga gggccgcgca ggccggtcag cttcttggcc acctggatgc ccaccagggt
     1621 caccagcccg gccaccgcgg ccaggtgcag gtagcggcgg gcccgctcgt tgaccaggcc
     1681 ggtcagcacc tcagcggtcc agatgccgag ggccggcagc agcagcaggc ccagggccag
     1741 ggcaacgatg tcgagccggt cggcccggcg gaacaagaag aactcagggc tgcggccggt
     1801 gacgtccagc agcggctggg cgatggcgaa gccgcagagc acgaacagct cgaccagcgc
     1861 ccacagctgc agccggtccc gcagcggccg ccggcgcggg ccgtcgccgt cctgaccggc
     1921 ccctgccggt ccagcccgca cctgctcgcc gtctcgggcc acgcgctccc tcagcctggt
     1981 gaggccgaga ccgactcgag gcggccgtcc agcagccgat ccaggaagtc ttggtcggcg
     2041 gctgggaccg cggcctcaag cggtccagcc agtcgacgca tgtcggtccc tagagcgtcg
     2101 tcgtcggtca tcgcgttaca cccgcgcca
//
LOCUS       DASSWR010000404         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_524685,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000404 DASSWR010000000
VERSION     DASSWR010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524685"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFO47_06725"
     CDS             <1..492
                     /locus_tag="VFO47_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8788424.1"
                     /translation="FCDDYLELVKDRAYRPAGEPGGASSRAALRTALDVQVRLLAPYL
                     PYACEEVWSWWRDGSVHLAAWPGPVSSPSAAGADRRPLDAAGQVIAAVRRAKSQAGVR
                     LRTPVELAEIAGPPPWLDAVRAAEADLAAAGRVGAFRYRERPGADGLDVRVALAAAEE
                     ATT"
     gene            489..1280
                     /locus_tag="VFO47_06730"
     CDS             489..1280
                     /locus_tag="VFO47_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET8788425.1"
                     /translation="MSEQPREVEGVLLVQADDQEAAGRRVADLEAVDRFDLRPRPPQR
                     IRDVYLDTGDGALAAARAAFRVRELDGRPLLTLKSAAVRTGLAAERLELEAPWSPEAL
                     QAVLGELRRQGVELPDPGEGAGAGEPLADLAALGLRPTQRRETSRTPRDVLERGHPDA
                     GPVAELTIDDVTFLLPAGRARLLEVEVEAKGPGGIETVQALLGALAAALPDDLRPWPY
                     GKLGTGRAVERLLAAGELDGLLGPDGRVRPAAHDRLAEFLAENSA"
     gene            1602..3029
                     /locus_tag="VFO47_06735"
     CDS             1602..3029
                     /locus_tag="VFO47_06735"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET8788426.1"
                     /translation="MLLPLLPRPGLRRLVPLVPLTGTTIDGVMDYQDAVDALYARMPT
                     RMGPTLERITRLAELLDHPERTAPAVHLTGTNGKTSTARMVASLLAAFGVGAGVYTSP
                     HLQDVRERVALATRLISTEEFAETWAYLEPFLAEVDRAGDQPVTFYEALTALAFLWFA
                     ELPVDAQVVEVGMGGTWDATNLVNGEVAIINRVALDHPELGGTPAAVASEKAGIIKRG
                     ATVVSHAQDDDVLAVIAERAAGLDARLLVAERDFGVERRRQAVGGQLLDLRTPSGTIT
                     DVLVPLHGRHQADNAAGALVAVEAFLGAHEGAWGPGTDAPASERRHLDHDTVRAGFAA
                     VTSPGRLEVMSRQPLVLLDGAHNPAGARALAAALLEEFVVDRRTLVVACLADKDIQGI
                     LRELAPATGRLVVTANRNPRAAPAERLRKEAEILGLHAEVAPDVATAVGRAIDGAAET
                     EAVVVTGSLHTVGEARDLLLGTGPA"
     gene            3075..>3254
                     /locus_tag="VFO47_06740"
     CDS             3075..>3254
                     /locus_tag="VFO47_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET8788427.1"
                     /translation="MADERTLVLCKPDAVARGLVGEVIGRLERKGFRLLAMELRTLDE
                     ATAKQHYGEHEGKPFF"
BASE COUNT          394 a         1232 c         1228 g          400 t
ORIGIN      
        1 ttctgcgacg actacctgga gctggtcaag gaccgcgcct accggcccgc cggcgagccg
       61 ggcggcgcct cgtccagggc ggcgctgcgc accgccctgg acgtccaggt gcggctgctc
      121 gccccctacc tgccgtacgc ctgcgaggag gtgtggtcgt ggtggcgcga cgggtcggtc
      181 cacctggccg cctggccggg cccggtgtcg tcgccgtccg ccgccggggc cgaccgccgg
      241 cccctggacg cggccgggca ggtgatcgcg gccgtccggc gggccaagag ccaggccggg
      301 gtgcggctgc gcacccccgt cgagctggcc gagatcgccg gcccgccccc gtggctggac
      361 gcggtccggg ccgccgaggc cgacctggcc gccgccggcc gggtgggggc gttccggtac
      421 cgcgagcgtc ccggcgccga cgggctcgac gtgcgggtcg cgctggccgc ggccgaggag
      481 gccaccacat gagcgagcag ccccgcgagg tcgagggggt cctgctggtc caggccgacg
      541 accaggaggc cgccgggcgg cgggtggccg acctggaggc cgtcgaccgc ttcgacctcc
      601 ggccgcggcc gccccagcgg atccgcgacg tctacctcga caccggcgac ggcgccctgg
      661 ccgcggcccg ggccgccttc cgggtccggg agctggacgg ccggccgctg ctcaccctca
      721 agtcggcggc ggtgcgcacc ggcctggccg cggagcggct ggagctggag gccccctggt
      781 cgcccgaggc cctccaggcc gtcctgggcg agctgcgccg ccaaggggtc gagcttcccg
      841 accccggtga gggcgccggg gcgggggagc cgctggccga cctggccgcc ctcggcctgc
      901 gcccgaccca gcgccgcgag acctcccgca ccccccggga cgtgctcgag cgtggacacc
      961 cggacgccgg gccggtggcc gagctgacca tcgacgacgt caccttcctg ctcccggccg
     1021 ggcgggcgcg gctgctggag gtcgaggtcg aggccaaggg cccgggaggg atcgagaccg
     1081 tgcaggcgct gctgggggcg ctggccgcgg ccctgccgga cgacctgcgg ccctggccgt
     1141 acggcaagct gggcaccggc cgggccgtgg aacggctgct ggccgccggc gagctggacg
     1201 gcctgctcgg ccccgatggc agagtccgcc cggccgccca cgaccggctc gccgaattcc
     1261 tcgcggaaaa ttccgcttga cgggtcgtca cggtcgcgcg caaactgctt gcacaagtct
     1321 ccagccagag aggcggtgaa cggcatgaag aggaacccgg gtcggacgat gctgctctgc
     1381 ggcagcgaat ggcgcatcca cctgcgtggt cgtgccgttc gagccgacat ctcgagcttc
     1441 gcgctccccg gagggaaccg gtcgtgaggc gcgcacgccg cgcctgattt cgcgctgacc
     1501 gcctccctcc cgggaggcgg tttttcgttt cccaatttcc gttccgcgtc cggggggagg
     1561 tgcgcaggag tgttccgcta cgcccaggag caggcccacc ggtgctactg ccgctactgc
     1621 cacggccagg gctgcggcgg ctggtgccgc tggtgccgct gaccgggact actatcgacg
     1681 gcgtcatgga ctaccaggac gccgtcgatg cgttgtacgc ccgcatgccg acccgcatgg
     1741 ggccgaccct ggagcggatc acgcggctgg ccgagctgct cgaccatccg gagcggaccg
     1801 cgccggcggt gcacctgacc gggaccaacg ggaagacctc gacggcccgg atggtcgcgt
     1861 cgctgctggc cgccttcggg gtcggggccg gggtctacac cagcccccac ctgcaggacg
     1921 tgcgcgagcg ggtggcgctg gcgacccggc tcatctcgac cgaggagttc gccgagacct
     1981 gggcctacct ggagccgttc ctggccgagg tcgaccgggc cggcgaccag ccggtgacct
     2041 tctacgaggc cctgaccgcc ctggccttcc tgtggttcgc cgagctgccg gtcgacgccc
     2101 aggtggtcga ggtcggcatg ggcggcacct gggacgccac caacctggtc aacggggagg
     2161 tggccatcat caaccgggtc gccctcgacc accccgagct cggcggcacc ccggccgcgg
     2221 tggccagcga gaaggccggc atcatcaagc gcggcgccac cgtggtcagc catgcccagg
     2281 acgacgacgt cctcgccgtc atcgccgagc gggcggccgg cctggacgcc cgcctgctgg
     2341 tcgccgaacg cgacttcggg gtcgagcggc gccgccaggc cgtcggcggc cagctcctcg
     2401 acctgcgcac ccccagcggg accatcaccg acgtgctggt gccgctgcac ggccgccacc
     2461 aggccgacaa cgccgccggg gccctggtcg cggtcgaggc gttcctgggc gcccacgagg
     2521 gagcgtgggg gccggggacc gacgcccccg cctcggagcg gcgccacctc gaccacgaca
     2581 ccgtccgggc cgggttcgcg gccgtcacct cgccggggcg cctggaggtg atgtcccgcc
     2641 agccgctggt gctgctcgac ggcgcccaca acccggccgg ggcgcgggcc ctggccgccg
     2701 cgctgctcga ggagttcgtg gtcgaccggc gcaccctggt ggtggcctgc ctggccgaca
     2761 aggacatcca ggggatcctg cgggagctgg ccccggccac gggccggctg gtcgtgacgg
     2821 ccaaccgcaa cccccgggcg gccccggccg agcggctgcg caaggaggcc gagatcctcg
     2881 gcctgcacgc cgaggtcgcc cctgacgtgg ccaccgccgt cggccgggcg atcgacggcg
     2941 ccgccgagac cgaggcggtc gtggtcaccg gctccctgca cacggtgggg gaggcgcgcg
     3001 acctgctcct gggcaccggc ccggcttgag tatgatcgcg cggtcatccc gaacccccgc
     3061 ccgtggagga ctccgtggcc gacgaacgca ccctggtcct gtgcaagccc gacgccgtcg
     3121 cccgcggcct ggtcggtgag gtgatcggcc ggctcgagcg caaggggttc cggctgctcg
     3181 ccatggagct gcgcaccctc gacgaggcga ccgccaagca gcactacggc gagcacgagg
     3241 gcaagccgtt cttc
//
LOCUS       DASSWR010000405         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_185661,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000405 DASSWR010000000
VERSION     DASSWR010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185661"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..395)
                     /locus_tag="VFO47_06745"
     CDS             complement(102..395)
                     /locus_tag="VFO47_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET8788428.1"
                     /translation="MVTAIVLVTCEVDQTPETAQALADLDGVSEVYSVAGDWDLVAVV
                     RVGGHDDLAATVTEHIRKVHGVASTRTLIAFRTYSRHDLDRMFSVGFEAGQPD"
     gene            complement(467..1636)
                     /locus_tag="VFO47_06750"
     CDS             complement(467..1636)
                     /locus_tag="VFO47_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788429.1"
                     /translation="MLAWTLWALMLAGLLLVAWLDGMLRRAGRPDLVSLGSDAWPYVL
                     AMVSAGTVGAVVASRRPRHPVGWLLLGLELSVIGLGIADAYAAYGLLARPGSLPGARW
                     AAIYVDESWVLLTTLLGFVLLLTPTGSLPSPRWRWWARLVVAAAVVGVLTSTQPLDPP
                     YAAVPRPLVVQDGPLATVAWLASVISLLGLLVAAGSLVVRFRRARGIERLQLRWVALA
                     AAGAGLAIAVILVTWALIGSAAQPLWEWATVLYLVGLPIATGASILRYRLYDLDRILS
                     RTLAWTVLTVLLGLGYATVVLLLGRLLPDSSSLGVAAATLAVAAMFQPAHRRIQGVVD
                     RRFDRHRYDAARTIAAFSTRLRGQVDLDTLSAELLAVADQTMQPTTASLWLRPRT"
     gene            complement(1809..>2853)
                     /locus_tag="VFO47_06755"
     CDS             complement(1809..>2853)
                     /locus_tag="VFO47_06755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET8788430.1"
                     /translation="DETVAGHGRQAGNGPNPARQVSVRAGVPETVPAYTVNKACGSSA
                     KALTLAAGSIMLGEIDVALVVGMENMTRTPYLLRGMRGGYRMGPAVIEDGMRSDGFLD
                     PLAGEVMGETAENLAERHAVSREEQDAFALQSQRRASEAWEAGRFAEEVVPVDTFDAR
                     RRTITVEHDEHPRPDTTLEGLARLEPVFRDPGTVTAGNSSGICDGAAALVVMAEETAR
                     SRGIRPLARIAGWASAGVDPHIMGWGVVPATRKLLAKLGWTLPEIDVVEVNEAFAAQV
                     LACDRELKFDWERTNVNGGAIALGHPIGMTGARIAGTVVHQLQDRGVRRGLATLCISG
                     GQGMAIALERVGD"
BASE COUNT          391 a         1050 c         1079 g          333 t
ORIGIN      
        1 cccgctcact cgacgagggc cgggcccgcg acaccctcga cgcctgggtc gagagctcgc
       61 gccgcgccgc cgccgacgcc tagggcctgt tatggacccg gtcagtccgg ctggccggcc
      121 tcgaagccga ccgagaacat gcggtcgagg tcgtggcggg agtaggtgcg gaaggcgatc
      181 agggtgcggg tcgaggcgac gccgtggacc ttgcggatgt gctcggtcac ggtggcggcc
      241 aggtcgtcgt ggccgccgac ccggaccacc gcgaccaggt cccagtcgcc ggccaccgag
      301 tagacctcgc tgaccccgtc gaggtcggca agggcctggg cggtctcggg ggtctggtcg
      361 acctcgcagg tgacgagcac gatggcggtg accaactccg aaccctccca cctcggcgaa
      421 cagagcggga ccgtaacagc ccgaccacgg ccggcgcaat cggccgtcag gtgcggggcc
      481 gcagccacag cgaggccgtg gtgggctgca tcgtctggtc ggcgacggcc agcagctcgg
      541 ccgagagggt gtccaggtcg acctgcccgc gcagccgggt gctgaaggcg gcgatggtgc
      601 gggcggcgtc gtagcggtgg cggtcgaagc ggcggtcgac cacgccctgg atgcggcggt
      661 gggccggctg gaacatcgcc gccacggcca gggtggcggc ggccacgccc aggctggagc
      721 tgtcgggcag cagccgcccc agcagcagca ccaccgtcgc gtagcccagg ccgagcagga
      781 cggtcaggac cgtccaggcc agggtgcggc tgaggatccg gtccaggtcg tacagccggt
      841 agcgcaggat cgacgctccg gtcgcgatcg gcaggccgac caggtagagc acggtcgccc
      901 actcccacag cggctgggcc gcgctcccga tcagcgccca ggtgaccagg atgaccgcga
      961 tcgccaggcc ggctccggcg gcggccaggg ccacccagcg aagctgcagc cgctcgatcc
     1021 cgcgggcgcg ccggaaacgc accaccagcg acccggccgc gacgagcagg ccgaggaggg
     1081 agatgaccga ggccagccag gccaccgtcg ccagcggccc gtcctggacg acgagcgggc
     1141 ggggcaccgc cgcgtacggc gggtccagcg gctgggtgct ggtcagcacc cccacgaccg
     1201 ccgcggccac gaccagcctg gcccaccagc gccagcgggg cgagggcagc gacccggtgg
     1261 gcgtcagcag cagcacgaac ccgagcaggg tcgtcagcag cacccagctc tcgtccacgt
     1321 agatggccgc ccagcgggcg ccgggcaggg acccgggtcg cgccagcagc ccgtaggcgg
     1381 cgtaggcgtc ggcgatcccg agcccgatca ccgacagctc gagccccagc agcagccacc
     1441 cgaccggatg ccgggggcgg cggctggcca ccaccgcccc gaccgtgccc gcgctgacca
     1501 tggccagcac gtacggccag gcgtcgctcc cgagcgacac cagatcgggc cgcccggccc
     1561 gccgcagcat cccgtccagc caggccacga gcagcagccc ggccagcatc agcgcccaga
     1621 gggtccaggc gagcaccgcc caggaccggg gtacctggtt cgttccgggg tcgccggcgg
     1681 gggcgggtcg gttccgccgg cgggtcggtc tcatgctcgg cacgcgcagc cctccgcctc
     1741 ccgcggtcct gccgcggcgt tggtgcggcc actgttgccg ccggccgtga cgggttcgtt
     1801 acggggtgtc agtcaccgac ccgctccagg gcgatggcca tgccctggcc gccggagatg
     1861 cagagggtgg cgaggccgcg gcgaacgccc cggtcctgga gctggtggac gacggtgccg
     1921 gcgatgcggg ccccggtcat gccgatcgga tggccgaggg cgatggcgcc gccgttgacg
     1981 ttggtccgct cccagtcgaa cttcagctcg cggtcgcagg ccagcacctg ggcggcgaac
     2041 gcctcgttga cctcgaccac gtcgatctcg gggagggtcc agccgagctt ggccagcagc
     2101 ttccgggtgg ccgggaccac gccccagccc atgatgtggg ggtcgacccc ggcgctggcc
     2161 cagccggcga tcctggccag gggacggatg ccccgggacc tggccgtctc ctcggccatc
     2221 acgaccaggg cggcggcgcc gtcgcagatc cccgaggagt tgccggcggt gaccgtgccc
     2281 gggtcgcgga acaccggctc gagcctggcc aggccctcca gggtggtgtc cggccgcggg
     2341 tgctcgtcgt gctcgacggt gatcgtgcgc cgccgggcgt cgaaggtgtc gaccgggacg
     2401 acctcctcgg cgaagcggcc ggcctcccag gcctcggacg cgcggcgctg cgactggagg
     2461 gcgaaggcgt cctgctcctc gcggctcacg gcgtggcgct cggccaggtt ctcggccgtc
     2521 tcgcccatga cctcgccggc cagcgggtcc aggaagccgt cggaccgcat cccgtcctcg
     2581 atcaccgccg gccccatccg gtagccgccg cgcatgcccc gcagcaggta cggggtgcgg
     2641 gtcatgttct ccatccccac gaccagggcg acgtcgatct cgccgagcat gatggagccg
     2701 gcggcgaggg tgagcgcctt ggcgctcgac ccgcaggcct tgttgacggt gtacgcgggg
     2761 accgtctcgg gcacgccggc ccggaccgac acctgccgcg ccgggttggg gccgttgccg
     2821 gcctgacggc cgtggccggc gacggtctcg tcg
//
LOCUS       DASSWR010000406         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_891964,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000406 DASSWR010000000
VERSION     DASSWR010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891964"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..2405
                     /gene="cofH"
                     /locus_tag="VFO47_06760"
     CDS             111..2405
                     /gene="cofH"
                     /locus_tag="VFO47_06760"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HET8788431.1"
                     /translation="MDISPGTPLDELLIEARRIRDRHHGTRVTYSPKVFIPLTMLCRD
                     HCGYCTFAKPPARLGTSPYLDPEQVLEIARAGAAAGCHEALFTLGERPEERYPVAREA
                     LGALGHDSTVDYLADACRLVLTETGLLPHANAGALLPAELAALRRVSASQGMMLESLS
                     ERLHEPGGPHARCPTKLPARRLATLEAAGELAIPFTTGILVGIGETRAERLEALAAIG
                     AAHRRHGHVQEVIVQNFRPKPGTAMAGAAPPALEDYLWTIAAARVLLDPRIHLQAPPN
                     LTEDFGVLLEAGIDDWGGVSPVTADHVNPEAPWPALERLRAVTEAAGMTLAPRLTIYP
                     ELAADPGRWLDPDLRFPVLDRSDAEALGRDDRWAAGDHEPPPILIDPVRFPGMAAVGR
                     VGEVLTGVVAGQQPGEDELAALFAARGPEVRAVAEVADDLRRQAVGDVVTFVRNRNIN
                     YTNVCTFRCRFCAFSKGPASLNLRGAPYLLGLDEVARRVVEADQRGATEVTLQGGIHP
                     SFDGDYYLHVAEAVHEAVPGMHIHGFTALEVHEGARRLGEPLRDYLLRLKAAGLASLP
                     GTAAEILDDEVRAVICPDKVTTDEWLEVHRVAHSVGLRSNITIMFGHVESPRHWARHL
                     LRTRALQAETGGFTEFVPLPFVHMAAPMYRKRQSRPGPTFREAVLIHAVGRIAYRGLI
                     DNIQVSWVKMGADGVRQALLAGVNDLGGTLMDENISRAAGASHGQMMQADDFRSIVEP
                     LGRTLEQRTTLYGRTRTEPAPVPG"
BASE COUNT          313 a          969 c          865 g          317 t
ORIGIN      
        1 tcgcgtcccg ggtcgcgcgt gggtcgcgtc ccaggtcgcg tcccgggtcg cgccgggatc
       61 gtgggcggga ttgcttgact ttttgaagtg atggcctgag gctgccgtac atggatatct
      121 ccccgggcac cccgctggac gagctgctga tcgaggcccg ccgcatccgc gaccgccacc
      181 acgggacccg ggtcacctac tcgccgaagg tgttcatccc cctgaccatg ctctgccgcg
      241 accactgcgg ctactgcacc ttcgccaagc ccccggcccg ccttgggacg tcgccctacc
      301 tggaccccga gcaggtgctg gagatcgcgc gggccggggc ggccgccggc tgccacgagg
      361 cgctgttcac cctgggggag cggcccgagg agcgctaccc ggtggcccgc gaggcccttg
      421 gcgccctcgg ccacgacagc accgtcgact acctggccga cgcctgccgg ctggtcctca
      481 ccgagaccgg gctgctgccc cacgccaacg ccggcgccct gctcccggcc gagctggccg
      541 ccctgcggcg ggtctcggcc agccagggga tgatgctgga gtcgctgtcg gagcggctcc
      601 acgagccggg cggcccccac gcccgctgcc cgaccaagct gccggcgcgc cggctggcca
      661 ccctggaggc ggcgggggag ctggccatcc cgttcaccac cgggatcctg gtcgggatcg
      721 gcgagacccg ggccgagcgg ctggaggcgc tggccgccat cggcgccgcc caccgccgcc
      781 acggccacgt gcaggaggtg atcgtccaga acttccgccc caagccgggc acggccatgg
      841 ccggggccgc gccgccggcg ctggaggact acctgtggac gatcgcggcc gcccgggtcc
      901 tgctcgaccc gcggatccac ctccaggccc cgcccaacct caccgaggac ttcggggtcc
      961 tgctggaggc gggcatcgac gactggggcg gggtctcgcc cgtcaccgcc gaccatgtca
     1021 acccggaggc gccctggccg gcgctcgagc ggctgcgggc ggtgaccgag gcggccggta
     1081 tgaccctggc cccgcggctc accatctacc cggagctcgc cgccgacccc gggcgctggc
     1141 tcgacccaga cctccggttc ccggtgctgg accgctccga cgccgaggcc cttggccgcg
     1201 acgaccgctg ggcggccggc gaccacgagc cgccgcccat cctgatcgac ccggtccggt
     1261 tccccggcat ggcggccgtc ggccgggtgg gggaggtcct gactggggtg gtggccgggc
     1321 agcagccggg ggaggacgag ctggccgccc tgttcgcggc caggggaccg gaggtgcggg
     1381 cggtggccga ggtcgccgac gacctgcgcc gccaggccgt cggggacgtg gtcaccttcg
     1441 tccgcaaccg caacatcaac tacaccaacg tctgcacctt ccgctgccgc ttctgcgcct
     1501 tctccaaggg cccggccagc ctcaacctgc gcggcgcgcc ctacctgctg ggcctggacg
     1561 aggtggcccg gcgggtggtc gaggccgacc agcgcggggc caccgaggtc accctccagg
     1621 gcggcatcca ccccagcttc gacggcgact actacctgca cgtggccgag gccgtccacg
     1681 aggccgtgcc cggcatgcac atccacggct tcaccgccct cgaggtccac gagggcgccc
     1741 gccggctcgg cgagccgctc cgcgactacc tgctgcggct caaggcggcc ggcctggcca
     1801 gcctgcccgg caccgccgcc gagatcctcg acgacgaggt ccgggccgtc atctgccccg
     1861 acaaggtgac caccgacgag tggcttgagg tccaccgggt cgcccactcg gtggggctgc
     1921 gctccaacat caccatcatg ttcggccacg tcgagtcgcc ccggcactgg gccaggcacc
     1981 tgctccggac ccgggccctg caggccgaga cgggcgggtt caccgagttc gtgccgctgc
     2041 cgttcgtgca catggccgcg cccatgtacc gcaagcgcca gtcccggccc gggcccacct
     2101 tccgggaggc ggtgctgatc cacgccgtcg ggcggatcgc ctaccgcggc ctgatcgaca
     2161 acatccaggt gtcgtgggtc aagatgggcg ccgacggggt ccgccaggcc ctgctggccg
     2221 gggtcaacga cctcggcggc acgctgatgg acgagaacat ctcccgggcc gccggcgcga
     2281 gccacggcca gatgatgcag gccgacgact tccgctcgat cgtcgagccg ctcgggcgca
     2341 cgctggagca gcgcaccacc ctctacggcc gcacccgcac cgagccggcc ccggtccccg
     2401 gctagcccga cccgcggggg tgctccagca ccaccagtcc gccggcgcac ttgccggtgg
     2461 tgcg
//
LOCUS       DASSWR010000407         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_439910,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000407 DASSWR010000000
VERSION     DASSWR010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439910"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFO47_06765"
     CDS             complement(<1..1026)
                     /locus_tag="VFO47_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788432.1"
                     /translation="MPDYKGRIFGRSVLDVRFRAFYGEDLFLGELLAGDDPERGRRYL
                     FRVTDVAYGSESSDAGWAERTAGGLMAMDQVGEATTLRDFEKRLYKVAEVVPLGYVDA
                     KGFHKPKSLPAQFAAVSAPTDADLAFLRERMGDVEVGRLRSGEDTIDLPVGIRGETLP
                     SHVGVFATTGMGKSNLMKVLAGQLLAAHGRYAMLLFDPHGEYLEGGAAGRRGLAHHPW
                     AQQRLRVYSPSPRAGQASLLRLSLAELTVDDLRTAWKFSGPQSEALESAYALLGDKGA
                     WLTRLAEEDPEVLKDAELGRHALATIQVLSRRAKRIVRLPLMTEDPSQSLTGKVLDDL
                     AAGKVVLI"
     gene            complement(1050..2075)
                     /locus_tag="VFO47_06770"
     CDS             complement(1050..2075)
                     /locus_tag="VFO47_06770"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HET8788433.1"
                     /translation="MLVEDYADRVRLAAGYLSRLLADGGTGVDNPADEPVGPADAAAL
                     ELATDAPLHQFGCDPAHGVTAAAVDGGSACLLNGRSFWLVAYRAGTVWHRDRDTFASD
                     VDSLQVRALTQADAKAAYAEALTLAGVRRERELADLGGVVDVLRELAEWRRAAEAVAA
                     ARPGDLVLVDGSLHPGPFLPAGLVEPVHELAREREVDLVGVTKASKLRWGRYAPLVLR
                     VRRRAEDELGPDARWYLRVTGPEETSEVYVARLARAGAYAFRVDAVRGHRDPDGLFAV
                     LAGLSDDPAFVGYPYPLAAVHRAVSLPGPLLADLRRDLREAFLREGLSEDDVDLVMRD
                     FHLTLNA"
BASE COUNT          313 a          842 c          898 g          285 t
ORIGIN      
        1 gatcagcacc accttgccgg cggccaggtc gtccaggacc ttgccggtga gcgactggct
       61 ggggtcctcg gtcatcaggg gcagccgcac gatccgcttg gcccgccggc tcagcacctg
      121 gatggtggcc agggcgtggc ggcccagctc ggcgtccttg aggacctcgg ggtcctcctc
      181 ggccaggcgg gtcagccacg cccccttgtc gccgagcagg gcataggcgc tctccagggc
      241 ctcggactgg gggccggaga acttccaggc ggtgcgcagg tcgtcaacgg tcagctcggc
      301 cagggacagc cgcagcaggc tggcctggcc ggcccggggg ctgggcgagt agacgcgcag
      361 gcgctgctgc gcccacgggt ggtgggccag gccccgccgg cccgccgctc ctccttcgag
      421 atactcgccg tgggggtcga acagcagcat ggcgtacctg ccgtgggcgg ccaggagctg
      481 gccggccagg accttcatca ggttgctctt gcccatgccg gtggtggcga acacgccgac
      541 gtggctgggc agggtctcgc cgcggatccc gaccggcagg tcgatggtgt cctcgcccga
      601 gcggagccgc ccgacctcga cgtcgcccat ccgctcgcgc aggaaggcca ggtcggcgtc
      661 ggtgggggcg gacacggcgg cgaactgggc gggcaggctc ttgggcttgt ggaagccctt
      721 ggcgtcgacg tagccgaggg ggacgacctc ggccaccttg tacagccgct tctcgaagtc
      781 gcgcagggtg gtggcctcgc ccacctggtc catggccatc aggccgccgg cggtgcgctc
      841 ggcccagccg gcgtccgacg actccgagcc gtaggcgacg tcggtgaccc ggaacaggta
      901 gcgccggccc cgctccgggt cgtccccggc cagcagctcg ccgaggaaca ggtcctcgcc
      961 gtagaaggcc cggaaacgca catcgagcac ggagcggccg aagatgcgtc ccttgtagtc
     1021 cggcatgtcc aggtctcccg tggtcgggct caggcgttca aggtcaggtg gaagtcgcgc
     1081 atcaccaggt cgacgtcgtc ctcggacagc ccctcgcgca ggaacgcctc gcgcaggtcg
     1141 cggcgcaggt cggccaggag cgggccgggc agcgacaccg cccggtggac ggcggccagc
     1201 gggtaggggt agccgacgaa ggccgggtcg tccgacagcc cggccaggac ggcgaacagc
     1261 ccgtcggggt cgcggtggcc ccggacggcg tcgacccgga aggcgtaggc cccggcccgg
     1321 gccaggcggg ccacgtacac ctcggaggtc tcctcggggc cggtgacccg caggtaccag
     1381 cgggcgtcgg ggccgagctc gtcctcggcc cggcgccgca cccgcagcac cagcggggcg
     1441 tagcggcccc agcggagctt ggacgccttg gtcaccccga ccaggtcgac ctcgcgctcg
     1501 cgggccagct cgtggacggg ctcgaccagc ccggcgggca ggaacgggcc cgggtggagg
     1561 ctgccgtcga ccagcaccag gtcgcccggg cgggcggccg cgaccgcctc ggcggcccgg
     1621 cgccactcgg ccagctcgcg cagaacgtcg accaccccgc cgaggtcggc cagctcgcgc
     1681 tcgcgcctga ccccggccag ggtcagggcc tcggcgtagg ccgccttggc gtcggcctgg
     1741 gtgagggccc tgacctggag cgagtcgacg tcgctggcga aggtgtcgcg gtcgcggtgc
     1801 cagaccgtcc ccgcccggta agcgaccagc cagaagctgc gcccgttgag caggcaggcc
     1861 gagccgccgt cgaccgcggc cgcggtcacc ccgtgggcgg ggtcgcagcc gaactggtgc
     1921 agcggggcgt cggtggccag ctccagggcg gcggcgtcgg cggggccgac cggctcgtcg
     1981 gccgggttgt cgacgccggt gccgccgtcg gcgagcagcc ggctgaggta gccggcggcc
     2041 agccgcacgc ggtcggcgta gtcctcgacg agcacgtgcc tcccctcgcc gcaccctgaa
     2101 cgaacacacg ttcaccccgg agggtaacca gaaaccctcg ggaggggaag caccgcggca
     2161 gtgtagctca ggcgaggggc tgttcgacca gcccggccag ccgcctgggg gcgaccagcc
     2221 ggtagctgtc ggcgaccagc tcggcgacct cgccccagtc gacgtcctgg aggtcgaggc
     2281 gcagccccac ccagccgcgg ttgccgacgt acctgggcag gtagaagcgg tgcgggtc
//
LOCUS       DASSWR010000408         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1259295, whole genome shotgun sequence.
ACCESSION   DASSWR010000408 DASSWR010000000
VERSION     DASSWR010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259295"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="whiA"
                     /locus_tag="VFO47_06775"
     CDS             complement(<1..803)
                     /gene="whiA"
                     /locus_tag="VFO47_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET8788434.1"
                     /translation="MSRGEFTVRVKEELAALRPAAPAERRALLAALLRFAATLHIGGT
                     LGPRYTLVLATGSGGVTRLAFWLLHTGYGVRPDFRVREAGALAPRARYELVLADRVER
                     VLTDSGILDGAGRLSFGVAGGLVRGREAAACFARGAFLGRGSVSAPTREPHLEVGAPE
                     ERTAADLAAVLVRLGLPARTGAHGPDEHRVVVKGGEAIGRALLLMGAQTAYLAWEDGR
                     IRREVRREAVRLANADQANLRRSVAAAMAQVAAVERAVARLGWDGLPADL"
     gene            complement(811..1698)
                     /gene="yvcK"
                     /locus_tag="VFO47_06780"
     CDS             complement(811..1698)
                     /gene="yvcK"
                     /locus_tag="VFO47_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HET8788435.1"
                     /translation="MRVVALGGGHGLAVTLAALRLLGVAPTAVVAVADDGGSSGRLRR
                     DLGLLPPGDLRKAMLALADPAAEVRELFGYRFERGDLAGHNLGNLALAALTDLKGGFQ
                     EALEEASRWLRVQGRVLPATLVPVRLCGLVDGRQVEGQVAIATASGRVESVWLEPRAP
                     AAVPAAVDAVRQADLVLLGPGSTFTSVVPNLLVPDLAAALTEAERLVYICNLEAQPGE
                     TSGFAPETHLAAILDHCPGLPVATVLCQRPQDAAAAARELRAFAELGAAVLHAEVAAD
                     DHAGRHDAARLATALKELA"
     gene            complement(1695..2594)
                     /gene="rapZ"
                     /locus_tag="VFO47_06785"
     CDS             complement(1695..2594)
                     /gene="rapZ"
                     /locus_tag="VFO47_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET8788436.1"
                     /translation="MTQELRTEDRLRAAAVEFVVITGLSGAGRSEAAKVLEDLGYFVI
                     DNLPASLIGRVAELAMVGRDRTRVALVMDARGGSFTAGVSELSTALNQLRAQDINLRV
                     LFLEASDEVLIRRYEATRRRHPVAGELVAESIALERDLLRELRADADLVLDTSDLNVH
                     ELRAKILAAFADEAAGLLVTVASFGFKYGLPLDADMVVDVRFLPNPHWVPELRPLPGT
                     DPAVRDYVLAQRDTGLFLARLEALLETALPGYVKEGKHYLTVAVGCTGGKHRSVVLAE
                     ELAGWLTDKGYRAHVAHRDVERE"
     gene            complement(2591..>3149)
                     /locus_tag="VFO47_06790"
     CDS             complement(2591..>3149)
                     /locus_tag="VFO47_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET8788437.1"
                     /translation="EGQDDVASLREVLTRRLARLASERNEPEEDGRRKKFAYPPNLIV
                     VDGGRPQLAAALEAVRASASPDLPVVSLAKRMEEVYLPGMPDPGVIPRTSEALYLLQR
                     VRDEAHRFAIAYHRKLRDRSMTASVLDGIPGVGESRRRQLVRHFGSARKAAQASLAEL
                     EQVPGLGPGLAKVVYDHLHGGARQE"
BASE COUNT          390 a         1181 c         1221 g          357 t
ORIGIN      
        1 aggtcggccg gcaggccgtc ccagccgagc cgggccaccg cccgctccac ggccgcgacc
       61 tgggccatgg ccgcggccac gctgcggcgc aggttggcct ggtcggcgtt ggccagccgc
      121 accgcctcgc ggcgcacctc ccgccggatg cggccgtcct cccaggccag gtaggcggtc
      181 tgggcgccca tgaggagcag ggcccggccg atggcctcgc cccctttgac caccacccgg
      241 tgctcgtcgg ggccgtgcgc gccggtccgg gcgggcaggc cgagcctgac caggacggcg
      301 gccaggtcgg cggcggtgcg ctcctcgggg gcgccgacct ccaggtgggg ctcgcgggtc
      361 ggggccgaca ccgagccgcg gcccaggaac gcgcccctgg cgaagcaggc ggccgcctcc
      421 cggcccctga ccaggccgcc ggcgacgccg aagctgagcc ggccggcgcc gtcgaggatg
      481 ccgctgtcgg tcagcacccg ctcgacccgg tcggccagga cgagctcgta gcgggcccgg
      541 ggggccagcg ccccggcctc gcggacgcgg aagtcgggcc ggaccccgta gccggtgtgc
      601 agcagccaga acgccagccg ggtcaccccg cccgacccgg tggccaggac cagggtgtag
      661 cgggggccga gggtgccgcc gatgtggagg gtggcggcga agcggagcag ggcggccagc
      721 agggcgcggc gctcggccgg ggcggccggc cgcagcgccg ccagctcctc cttcacccgg
      781 acggtgaact cgccgcggct catcggcgcc ctaggcgagt tccttgaggg cggtggccag
      841 gcgggccgcg tcgtggcggc cggcgtggtc gtcggcggcc acctcggcgt ggaggacggc
      901 cgccccgagc tcggcgaagg cccgcagctc gcgggccgcg gcggccgcgt cctggggccg
      961 ctggcagagc acggtggcta ccggcaggcc cgggcagtgg tcgaggatgg cggccaggtg
     1021 ggtctcgggc gcgaagccgc tggtctcccc cggctgggcc tccaggttgc agatgtagac
     1081 cagccgctcg gcctcggtca gggccgcggc caggtcgggg acgagcaggt tgggcacgac
     1141 cgaggtgaag gtcgacccgg ggccgagcag gaccaggtcg gcctggcgga cggcgtcgac
     1201 cgcggccggc acggcggccg gggcgcgcgg ctccagccac accgactcga cccggccgct
     1261 ggccgtggcg atcgccacct ggccctcgac ctggcggccg tcgaccaggc cgcacagccg
     1321 caccggcacc agcgtggccg gcaggacccg cccctggacc cggagccacc gcgacgcctc
     1381 ctccagcgcc tcctggaagc cgcccttgag gtcggtcagg gcggccaggg ccaggttgcc
     1441 caggttgtgg ccggccaggt cgccgcgctc gaaccggtag ccgaacagct cgcggacctc
     1501 ggccgccggg tcggccaggg cgagcatggc cttgcgcagg tcgcccggcg ggagcagccc
     1561 gaggtcgcgg cgcagccgcc ccgaggagcc gccgtcgtcg gccacggcca ccaccgcggt
     1621 cggagccacc ccgagcagcc gcagggcggc cagggtgacg gccaggccgt ggccgccccc
     1681 gagcgcgacg accctcactc gcgctccacg tcccggtggg cgacgtgggc ccggtagccc
     1741 ttgtcggtca gccagccggc cagctcctcg gccaggacca cgctgcggtg cttgcccccg
     1801 gtgcagccaa cggccaccgt caggtagtgc ttgccctcct tgacgtagcc gggcagggcc
     1861 gtctccagca gcgcctccaa ccgggccagg aacaggccgg tgtcgcgctg ggcgagcacg
     1921 tagtcgcgca cggccgggtc ggtgccgggc agcgggcgca gctccggcac ccagtgcggg
     1981 ttgggcagga agcggacgtc gaccaccatg tcggcgtcca ggggcagacc gtacttgaag
     2041 ccgaaggagg ccaccgtgac cagcaggccg gcggcctcgt cggcgaacgc ggccaggatc
     2101 ttggcccgca gctcgtgcac gttgaggtcg ctggtgtcga ggaccaggtc ggcgtcggcc
     2161 cgcagctcgc gcagcaggtc gcgctccagg gcgatcgact cggcgaccag ctcgccggcc
     2221 accgggtggc gccggcgggt ggcctcgtag cggcggatga ggacctcgtc ggaggcctcc
     2281 aggaacagca cccggaggtt gatgtcctgg gccctgagct ggttgagggc ggtcgacagc
     2341 tcggacacgc cggccgtgaa ggagccgccg cgggcgtcca tgaccagcgc cacccgggtc
     2401 cggtcgcggc cgaccatggc cagctcggcg acccgcccga tcagcgacgc cggcaggttg
     2461 tcgatcacga agtagccgag gtcctccagc accttggccg cctccgagcg gccggccccc
     2521 gacagcccgg tgatcaccac gaactccacc gcggccgcgc gcaggcggtc ctcggtccgc
     2581 agctcctggg tcattcctgt ctggcccccc catggagatg gtcgtagacc accttagcaa
     2641 gccccgggcc caggccgggg acctgctcca gctcggccag gctggcctgg gcggccttgc
     2701 gggccgagcc gaagtggcgt accagctggc gccgtcggga ctcgccgacc ccggggatgc
     2761 cgtcgagcac gctggccgtc atggaccggt cccgcagctt gcggtggtag gcgatggcga
     2821 agcggtgggc ctcgtcgcgg acccgctgca gcaggtagag ggcctcgctg gtccggggga
     2881 tgacccccgg gtcgggcatg cccggcaggt acacctcctc catccgcttg gccagcgaca
     2941 ccaccggcag gtcggggctg gccgaggccc gcaccgcctc cagggcggcg gccagctggg
     3001 gccggcctcc gtcgaccacg atcaggttcg gcgggtaggc gaacttcttg cggcgcccgt
     3061 cctcttccgg ctcgttccgc tcgctggcca ggcgggccag gcggcgggtc aggacctcgc
     3121 gcagggaggc gacgtcgtcc tggccctcg
//
LOCUS       DASSWR010000409         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_665984,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000409 DASSWR010000000
VERSION     DASSWR010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665984"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFO47_06795"
     CDS             <1..708
                     /locus_tag="VFO47_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788438.1"
                     /translation="ARDLARLWEVAMRRADFRAIVGTRAARLPGGPLSLRRFVTTNQL
                     LGAYRWTVGGKTGFTNRAGRCLVASASRGGRRLVAVALGSPNAFPDVQALFDYGFSKF
                     VRVRLAERGQPVSLAPGRPAALQTGADTDALVRLDELAQVRLAPAPGSTGGSAWFTGG
                     GRRLVPVRLVRLPVGGPGSTSPVPAGPSTTAAVAGAGPAGPPGATLSAGAVVPVGPVP
                     PGAPPPAIDPFLRREPP"
     gene            789..1358
                     /gene="hpt"
                     /locus_tag="VFO47_06800"
     CDS             789..1358
                     /gene="hpt"
                     /locus_tag="VFO47_06800"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET8788439.1"
                     /translation="MVRLLVDSETVQERVGELGAELRADYRGVEPILVSVLRGGVVFL
                     ADLVRAAAMPCRVDFMAVSRFDASEDTGVVRIEKDLDLDLTGAHVLVVEDIVDTGLTL
                     TYLLRVLAARGPASLRVCALLDKRARRIVDVPLSYIGFEVPDVFLVGYGLDLDERERG
                     RGELLAVDDLEAVRADPALLDLPTRPDGA"
     gene            1501..1974
                     /locus_tag="VFO47_06805"
     CDS             1501..1974
                     /locus_tag="VFO47_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET8788440.1"
                     /translation="MVELTLVGVRIELPTQTPIVLLKERDGERFLPIWIGNVEATAIA
                     FALQGVATQRPLTHDLLKNLLDELVVSIERVVITELKEGTFYATIELRQNGSKFSVSS
                     RPSDAIALAVRANTPIFAEEDVLSEASILIKSDDEEREVEKFREFLDQVSPEDFE"
     gene            2120..>2332
                     /locus_tag="VFO47_06810"
     CDS             2120..>2332
                     /locus_tag="VFO47_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8788441.1"
                     /translation="MAARPSPAASEPGFRGPIVYKLVGITYRQLDYWARTDLIRPSLA
                     DARGSGSRRLYSYRDLLELKLVKTLLD"
BASE COUNT          292 a          865 c          823 g          352 t
ORIGIN      
        1 gcccgcgacc tggcccgcct ctgggaggtg gccatgcgcc gggccgactt ccgcgccatc
       61 gtcggcacca gggccgccag gctgcccggt ggcccgttgt cgcttcgtcg atttgtcacc
      121 acgaaccagc tgctcggcgc ctaccggtgg acggtcgggg gcaagaccgg gttcaccaac
      181 cgggccgggc gctgcctggt cgcctcggcc agccggggcg gccgccgcct ggtcgcggtc
      241 gcgctcggct cgcccaacgc cttcccggac gtgcaggccc tgttcgacta tggcttctcc
      301 aagttcgtcc gggtccgcct ggccgaacgc ggccagcccg tctccctggc ccccggccgc
      361 ccggccgccc tccagaccgg cgccgacacg gacgccctgg tccgcctgga cgagctcgcc
      421 caggtccgcc tggcccccgc ccccgggtcg accggcggga gcgcctggtt cacgggtggg
      481 gggcggcggc tcgtcccggt ccggctggtc cggctcccgg tgggcgggcc cggatcgacc
      541 tcgccggtcc cggctggtcc atccaccacc gccgccgtgg cgggcgccgg gccggccggg
      601 ccgccgggcg cgaccctcag cgcgggagcg gtcgtcccgg tcgggcccgt cccacccgga
      661 gccccgccgc cggcgatcga cccgttcctc cgccgcgagc ccccgtgacc ccgcccgggg
      721 accccacccc cgacccgccc tccccggcga cctcacccgg gccggaccct ggggtcgagg
      781 aacgacccgt ggtgcggttg ctggtggact cggagacggt gcaggagcgg gtcggggagc
      841 tgggggccga gctgcgggcc gactaccggg gggtggagcc gatcctggtg tcggtgctgc
      901 gcggtggggt ggtgttcctg gccgacctgg tgcgggcggc ggcgatgccg tgccgggtgg
      961 acttcatggc cgtctcgcgc ttcgacgcct ccgaggacac cggggtggtc cggatcgaga
     1021 aggacctcga cctggacctg accggcgccc acgtgctggt ggtggaggac atcgtcgaca
     1081 ccggcctgac cctgacctac ctgctccggg tgctggccgc ccgcgggccg gccagcctgc
     1141 gcgtctgcgc cctgctggac aagcgggccc ggcgcatcgt ggacgtcccc ctctcctaca
     1201 tcggcttcga ggtcccggac gtgttcctgg tcgggtacgg gctggacctg gacgagcgcg
     1261 agcgggggag gggcgagctg ctggccgtcg acgacctcga ggcggtccgc gccgacccgg
     1321 ccctgctcga ccttccgacc aggcccgatg gcgcgtgacg gccgcctggg gcgtggacgg
     1381 ctcccggctg caaccttcgc tcggtatgtc gcatcggctg ggggcgtctg atagcctcgg
     1441 gcagtgcggc aagcgcccgg ccgggcgcca ggaacgttcg acgcccgagg gggtttcgcg
     1501 ttggtcgaac tgaccctcgt cggcgtcagg atcgagcttc ccacccagac gccgatcgtc
     1561 ctcctgaagg agcgcgacgg cgagcgcttc ctccccatct ggatcggcaa cgtggaggcc
     1621 acggccatcg ccttcgccct ccagggcgtg gccacccagc ggcccctcac ccacgacctg
     1681 ctcaagaacc tgctcgacga gctggtggtc tcgatcgagc gggtcgtgat caccgagctc
     1741 aaggaaggca ccttctacgc caccatcgag ctgcgccaga acggctccaa gttctcggtg
     1801 tcgagccggc cgagcgacgc catcgccctg gccgtccggg ccaacacgcc catcttcgcc
     1861 gaggaggacg tgctcagcga ggcctccatc ctcatcaaga gcgacgacga ggagcgcgag
     1921 gttgagaagt tccgcgagtt tctcgatcag gtcagtcccg aggatttcga gtaggtctgg
     1981 aggggtttca tcctctgctg gaggtcgaga gatcgcagct cggtaacggt ggggttgctt
     2041 cgttgacgca gccgacgcag acccctaggc tggacgtcac atcgttgtaa ttacgcagga
     2101 tccggcaagg aggaactgga tggccgcgcg accgtccccg gccgcatctg agccgggctt
     2161 ccgcggcccc atcgtctaca agctggtcgg catcacctat cggcagctcg actactgggc
     2221 ccgcaccgat ctgatccgcc cgtcgctcgc cgacgcccgt ggcagcggca gccgccgcct
     2281 gtacagctat cgcgacctgc tcgagctcaa gctggtcaag accctgctcg ac
//
LOCUS       DASSWR010000410         5030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1201938, whole genome shotgun sequence.
ACCESSION   DASSWR010000410 DASSWR010000000
VERSION     DASSWR010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201938"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1217
                     /locus_tag="VFO47_06815"
     CDS             174..1217
                     /locus_tag="VFO47_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788442.1"
                     /translation="MSNRIHRRAGLVAGLALLVVAMTAGPAAAHALGTGTEASNYRTT
                     VHGIEKGTPGLSVRTLAGGQLELTNRSPQEVLVLGYRLEPYLRVGPDGVFENQRSPST
                     YTNRFTTAPATIPPEFNPDADPEWKRIADGPTVVWHDHRAHWTGPDPAAVTASPGTVP
                     VVVPRWQIPLRQGSRTMVVRGDVTWVPGPSPWPWALAAVALFAAALAAAGGYRRPKVL
                     AVVALVAVAADMVHTAGALLASTAPVAAELYGVLTSVAGWVVAGLAAWRLLRGRADTG
                     LIYLLLGGVFLTLAGALPDLSALARSQLASGFGPVATRAAITFTLGLGAAMVVAGLAG
                     FRAPRRSQPASVS"
     gene            1222..2232
                     /locus_tag="VFO47_06820"
     CDS             1222..2232
                     /locus_tag="VFO47_06820"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8788443.1"
                     /translation="MVRSLKLGRRAVAVSALALAGLAVPGAGAALAGPAPGSQVPAPT
                     PGRLLLAWSLNPLVTVGLLAATIAYLQARRRLVADGVAWPARRAAYFLCGIGALALAL
                     LSPIEAYDTVLFSVHVGQHMLLTMVAAPLLALGAPVTLALRVAKGKTRRRMVRVLHSP
                     PLRVLGHPLVAWVLFTVTLYGLYFSPLFDLTLREPLVHDLVHLHFLTAGLLFWWVVVG
                     LDPSRWRLPHIARLLFVFLMVPFHAFLGVAIMNSGRVLAPTLETFQRDWGPTPLADQQ
                     AGGAILWGAGDLIALVAVLGVLISWASYEEKVVNVREDRRLARERAAGRPPHPVSRPP
                     VP"
     gene            2229..3011
                     /locus_tag="VFO47_06825"
     CDS             2229..3011
                     /locus_tag="VFO47_06825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8788444.1"
                     /translation="MSDPIPDPPGDPPGPDLTGKVRAWWDADAATYDDSTDHGWAAAS
                     PALRAAWNAALDRLLPGPGARVLDVGAGTGFLSLAAARLGHRVTALDLSGGMLERLQG
                     KAAADGLEVELVEGGAAEPPPGPFDAVVERHLLWTLPDPGAALAAWRGVAPQGRLVLF
                     EGLWGGADPLEALRGRAREGLRRLRGEPDHHHDHYDPAVLEELPLSGGTHPDRLVELV
                     EAAGWGPARLERLRDVEWAHLLALPASVRLLGVTPRFAVVAG"
     gene            3049..3390
                     /locus_tag="VFO47_06830"
     CDS             3049..3390
                     /locus_tag="VFO47_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET8788445.1"
                     /translation="MATDLICPKCQGHMRTYERSGVTVDQCGDCRGIFLDRGELERLV
                     DAEQGWQRSHSAPRDRDYERERDYERGRDRGSSFPIPFGGDDDDDDDRRRYSQGSHRK
                     RKKSFFDELFD"
     gene            3458..3994
                     /locus_tag="VFO47_06835"
     CDS             3458..3994
                     /locus_tag="VFO47_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788446.1"
                     /translation="MALMVRSSLGVMPWDVLHQGLARQLGWSLGTVTIVVGTLVLLAW
                     IPLRERPGLGTLSNVVVVGLAVDAVLAVLAAPEGLAWRAAFAASGILLNGVATAAYIG
                     VDLGPGPRDGLMTGLVRRTGGPVGLVRTAIEVVVVATGWLLGGTLGVVTVLYALTIGP
                     LVHVLLPRLTVAPARAAC"
     gene            complement(3991..4794)
                     /locus_tag="VFO47_06840"
     CDS             complement(3991..4794)
                     /locus_tag="VFO47_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET8788447.1"
                     /translation="MGIAVRHLGRATLLGVGALATTLAAEVMAARRHPFVPSHPPLEI
                     SGTIGPEDGEPLTLVVLGDSSVAGVGADAAEDTLTYGVAKALSDQYLVSLHALGVSGS
                     RLADVVGKQLPQVDGLEPDIVLVCVGTNDVTHGTRLREARRQLRLLVDGLAEVAPDAK
                     VIVSGLPPAETATAFHRPLRDLIGLRALLFTRLYRAELTPHGISVFDIAKLTKSAFHG
                     KREMFSADRFHPSSAGYAFLGTIYGRAVREAIEAAAMDLKADPEAELAS"
BASE COUNT          548 a         1887 c         1898 g          697 t
ORIGIN      
        1 accgggtatg tcctggtcgg cgtgccgccg tcggccggcg ggtcggacgg tgactcgcgc
       61 aaccggatcc tgctgctggg cacggggatc gcgctcgtga tcgtggccgc cgccgtggtc
      121 ctccgcctcc gcgagcggtc gcggcccgcg cccccggacc gggggcgccg acggtgagca
      181 accgcatcca ccgccgggcc gggctggtcg ccgggctggc cttgctggtg gtcgccatga
      241 cggccgggcc ggcggccgcg cacgccctcg ggaccgggac ggaggcgagc aactaccgga
      301 ccaccgtcca cggcatcgag aagggaacgc ccggcctgtc ggtccgcacc ctggccggcg
      361 gccagctgga gctgaccaac cggtcgcccc aggaggtgct ggtcctcggc taccgcctgg
      421 agccgtacct gcgcgtcggg cccgacgggg tgttcgagaa ccagcggtcg cccagcacct
      481 acaccaaccg cttcaccacc gccccggcca ccatcccgcc cgagttcaac cccgatgccg
      541 acccggagtg gaagcgcatc gccgacgggc cgacggtggt ctggcacgac caccgggccc
      601 attggaccgg acccgacccg gcggccgtga ccgccagccc cgggacggtc cctgtggtcg
      661 tgccccgatg gcagatcccg ctgcgccagg gcagccggac gatggtggtc cggggcgacg
      721 tcacctgggt gcccgggccg tcgccctggc cgtgggcgct ggccgcggtc gcgctgttcg
      781 ccgccgccct ggccgccgcc ggcggctacc gccggcccaa ggtcctggcc gtggtcgccc
      841 tggtcgccgt ggccgccgac atggtgcaca cggccggcgc gctgctcgcc tccaccgcgc
      901 cggtcgcggc cgagctgtac ggggtcctca cctcggtcgc ggggtgggtc gtggccgggc
      961 tggccgcctg gcgcctgctc cgcggccggg ccgacaccgg cctgatctac ctgctgctcg
     1021 gtggcgtgtt cctcaccctg gccggggccc tgcccgacct gtcggctctg gcccgctccc
     1081 agctcgcctc cggcttcggc ccggtcgcca cccgggccgc catcaccttc accctcggcc
     1141 tgggggcggc catggtggtc gcgggcctgg ccgggttccg ggcgccgcgc cggtcccaac
     1201 ccgccagcgt aagctgaggt cgtggtgcgt tctctgaagc tcgggcgccg ggcggtggcc
     1261 gtcagcgcgc tcgcgctggc cggcctggcc gtccccggcg cgggcgccgc cctggccggc
     1321 cccgcccccg ggtcgcaggt gccggccccg accccgggcc ggctgctgct cgcgtggtcg
     1381 ctcaaccccc tggtcaccgt cggcctgctg gccgcgacga tcgcctacct ccaggcccgg
     1441 cggcgcctgg tcgccgacgg ggtggcctgg ccggcgcggc gagccgccta cttcctgtgc
     1501 gggatcggcg ccctggccct ggccctgctg tccccgatcg aggcctacga cacggtgctg
     1561 ttctcggtcc acgtcggcca gcacatgctg ctcaccatgg tcgccgcccc gctgctggcc
     1621 ctgggcgccc cggtcaccct ggccctgagg gtggccaagg ggaagacccg ccgccgcatg
     1681 gtccgggtgc tgcacagccc accgctgcgg gtgctcggcc acccgctggt cgcctgggtg
     1741 ctgttcaccg tcaccctgta cgggctctac ttctcgccgc tgttcgacct gaccctgcgc
     1801 gagccgctgg tccacgacct ggtgcacctg cacttcctca ccgccgggct gctgttctgg
     1861 tgggtggtgg tcggcctcga cccgtcccgc tggcgcctgc cccacatcgc ccggctgctg
     1921 ttcgtgttcc tgatggtgcc gttccacgcc ttcctcgggg tggcgatcat gaacagcggg
     1981 cgggtgctgg ccccgaccct ggagaccttc cagcgcgact gggggccgac cccgctggcc
     2041 gaccagcagg ccggcggggc catcctctgg ggggccggcg acctgatcgc cctcgttgcg
     2101 gtgctgggcg tgctcatctc ctgggccagc tacgaggaga aggtggtgaa cgtccgcgag
     2161 gaccgccgcc tggcccgcga gcgcgccgcc ggccgtcctc cccaccccgt cagccggccc
     2221 ccggtcccgt gagcgacccg atccccgatc ccccggggga cccccccggg ccggacctca
     2281 ccggcaaggt tcgcgcctgg tgggacgctg acgccgccac ctacgacgac tccaccgacc
     2341 acggctgggc cgcggcctcg ccggcgctcc gggccgcctg gaacgccgcc ctggaccgcc
     2401 tgctccccgg cccgggcgcg cgggtgctgg acgtgggcgc cgggaccggg ttcctgtcgc
     2461 tggccgcggc caggctgggg caccgggtca cggccctcga cctctcgggc ggcatgctgg
     2521 agcggctgca ggggaaggcc gccgccgacg ggctcgaggt cgagctggtc gagggcggcg
     2581 ccgcggagcc gccgcccggg ccgttcgacg ccgtggtcga gcgccacctg ctgtggacgc
     2641 tgcccgaccc gggggccgca ctcgccgcct ggcggggggt ggccccgcag ggacggctgg
     2701 tgctgttcga ggggctgtgg ggcggggccg acccgctcga ggccctgcgc gggcgggccc
     2761 gggaggggct gcgccggttg cgcggggagc cggaccacca ccacgaccac tacgacccgg
     2821 ccgtcctgga ggagctgccg ctgtccggcg ggacccaccc cgaccggctg gtcgagctgg
     2881 tcgaggcggc cggctggggt ccggcccggc tggagcggct gcgcgacgtc gagtgggccc
     2941 acctgctcgc cctgccggcc agcgtccgcc tgctcggggt gacgccgcgg ttcgcggtgg
     3001 tcgccggctg aactagactc gaaccgccga cacaaggagg cgacctccgt ggccaccgac
     3061 ctgatctgcc ccaagtgcca gggacacatg cggacctacg agcgcagcgg cgtcaccgtc
     3121 gaccagtgcg gcgactgccg cggcatcttc ctggaccggg gcgagctcga gcgcctggtc
     3181 gatgccgagc agggctggca gcgcagccat tccgcgcccc gcgaccggga ctacgagcgc
     3241 gagcgcgact acgagcgggg acgcgaccgc gggtcgagct tcccgatccc gttcggcggc
     3301 gacgacgacg acgacgatga ccgccgccgc tacagccagg gctcccacag gaagcgcaag
     3361 aagagcttct tcgacgagct cttcgattag ccacgaccgg cgcctgccgc ggcggctggt
     3421 gcagctgtac gccgggctgg tgctctacgg cgtgtcgatg gcgctgatgg tgcgctcctc
     3481 gctcggggtg atgccctggg acgtgctgca ccagggcctc gcccgccagc tgggctggtc
     3541 gctggggacg gtcacgatcg tcgtcgggac gctggtgctg ctggcctgga taccgctgcg
     3601 cgagcggccg gggctcggca ccctcagcaa cgtcgtggtc gtggggctgg ccgtggacgc
     3661 ggtcctggcc gtgctggcgg cgccggaggg actggcctgg cgggccgcct tcgccgcctc
     3721 gggcatcctg ctcaacggcg tggccacggc cgcctacatc ggggtcgacc tggggccagg
     3781 gccgcgcgac gggctcatga ccgggctggt gcggcgcacc ggcggcccgg ttggcctggt
     3841 ccggacggcc atcgaggtcg tggtggtcgc gaccggctgg ctgctgggcg gcaccctcgg
     3901 ggtcgtgacc gtgctctacg ccctgaccat cgggccgctg gtccacgtgc tcctgccccg
     3961 gctcacggtc gccccggccc gagcggcctg ctagctggcg agctcggcct cggggtcggc
     4021 cttgaggtcc atggcggcgg cctcgatggc ctcgcggacg gcgcggccgt agatggtgcc
     4081 gaggaaggcg tagccggcgc tggaggggtg gaaccggtcg gcgctgaaca tctcccgctt
     4141 gccgtggaag gccgacttgg tcagcttggc gatgtcgaac accgagatgc cgtgcggggt
     4201 cagctcggcc cggtacaggc gggtgaagag cagcgcccgg aggccgatca ggtcgcgcag
     4261 gggccggtgg aaggcggtcg cggtctcggc cgggggcagg ccggagacga tcaccttggc
     4321 gtcgggggcg acctcggcca ggccgtcgac cagcagccgg agctggcggc gggcctcacg
     4381 gagcctggtc ccgtgggtca cgtcgttggt gccgacgcag accagcacga tgtccggctc
     4441 caggccgtcg acctggggga gctgcttgcc gacgacgtcg gccaggcgcg acccggagac
     4501 gccgagggcg tggaggctga ccaggtactg gtcgctgagg gccttggcca ccccgtaggt
     4561 gagggtgtcc tcggcggcgt cggcacccac cccggcgacc gagctgtcgc cgaggacgac
     4621 cagcgtaagt ggctcgccgt cctccgggcc gatggtgccg ctgatctcca gcggcgggtg
     4681 ggagggaaca aacgggtggc gccgggccgc catgacctcg gcggccagcg tggtcgccag
     4741 cgcgcccacc ccgagcaggg tcgcccgacc aaggtgccgg acagcgatgc ccaacagcgc
     4801 ctcctcgtac gagtccagcg tacgggttgc tgaccggttt gtgaagcctg caaccaccgt
     4861 acgcggcgct gtgtcgcgga ccgtcacctc acgatctggc tacagccggt gcagggccga
     4921 cgggtcgtcc ccggcgatcg gggcgtccca caggtcctcc aggccggcca gggggctgga
     4981 gggttcagtg gccgatcgcc gggcgggcgc ctaccatccc gccatgcccg
//
LOCUS       DASSWR010000411         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1231549, whole genome shotgun sequence.
ACCESSION   DASSWR010000411 DASSWR010000000
VERSION     DASSWR010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231549"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..822)
                     /locus_tag="VFO47_06845"
     CDS             complement(70..822)
                     /locus_tag="VFO47_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET8788448.1"
                     /translation="MKILVTVKRVPDTAAEKRLDPADKTLDRESVETILNPVDEYAVE
                     EALRLKEQHGGEVVVLCMGPASALATVRKALSMGPDAAVHVHDDALHGSCAFNTAKVL
                     QAAVRQVEPDIVVCGSESTDARTSLVPSALAEYLGWPGLTGAKQLEVDGQTVRVHRET
                     ENGYDVLEADGPAVVSVVKGIMAAKSKPVTDYTLADLGIDPGEVGAAGATTEVVDFAP
                     RPPRQAGQQVKDPGDGSAAVELAEFLASHKFI"
     gene            945..1961
                     /locus_tag="VFO47_06850"
     CDS             945..1961
                     /locus_tag="VFO47_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET8788449.1"
                     /translation="MARILPPPALPNAPGELRQDPLSGGWVAITVGRAARPEAFLGDV
                     GTPRGPLGCPFCPGNEHMTPPEVWADRDPGTEPDKPGWHVRVVPNKFPAFAGPPAEAR
                     NNGGLYRSVPTAGVHEVVIHNPDHEATLADLPPAAVARAMAAWRGRLAVHHDQAFGAV
                     LVIVNQGRTAGASLEHPHSQVFATAGRPDRVRAEVDRLAGDACAACATVAAERGGPRL
                     VGANGDLLTLCPWASAAPFEALLLPAGHRPRFDEGGHDDTAMAASLRDLLRRFRGVAG
                     DHAPYNLVLHSAPPGVDDFHWHLHLLPRLTTYGGFELGTGVIINVVDPDRAADALRSA
                     ANGS"
     gene            complement(1948..>2283)
                     /locus_tag="VFO47_06855"
     CDS             complement(1948..>2283)
                     /locus_tag="VFO47_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8788450.1"
                     /translation="LVGPAKAKEMIYSGRFYGAAECQAMGLVDQVHPAGEVYQRACEA
                     AAGYAKGPGLALRAAKQVIDGGLEAGIDAGLLLERQAFHSLFATEDAAEGMASFVANG
                     PGKATFRNR"
BASE COUNT          280 a          877 c          809 g          317 t
ORIGIN      
        1 ttcctggtga cgccgtcctc atgctcgacc agaacgagaa ctctcggcat cgcgttacgc
       61 tccccgatct cagatgaact tgtggctggc caggaactcg gccagctcga ccgcggcgct
      121 gccgtcccca gggtccttga cctgctgacc cgcctgcctc gggggccgtg gcgcgaagtc
      181 gacaacctcg gtggtggcgc cggcggcgcc gacctcgccc gggtcaatac ccaggtcggc
      241 cagggtgtag tcggtcaccg gcttggactt ggcggccatg atgcccttga ccacgctgac
      301 cacggccggg ccgtcggcct cgagcacgtc gtagccgttc tcggtctcac ggtggacgcg
      361 gacggtctgg ccgtccactt cgagctgctt ggccccggtc aggccgggcc agcccaggta
      421 ctcggccagg gcgctcggga ccagggaggt gcgggcgtcg gtcgactccg acccgcagac
      481 cacgatgtcc ggctcgacct ggcggacggc cgcctggagc accttggccg tgttgaaggc
      541 gcacgacccg tggagggcgt cgtcgtgcac gtggacggcc gcgtccggcc ccatggagag
      601 cgccttgcgc accgtggcca gggcggaggc cgggcccatg cagagcacca cgacctcgcc
      661 gccgtgctgc tccttgagcc gcagggcctc ctcgacggcg tactcgtcca ccgggttgag
      721 gatcgtctcg accgactcgc ggtcgagcgt cttgtccgcc gggtcgagcc gcttctcggc
      781 ggccgtgtcg ggcacgcgct tgacggtgac caggatcttc aacgctgcct ccaggggtgg
      841 ccggtgccgg accgtgtccg ggccggcgac catgatgccg caacgggccg accctttgcc
      901 tgtccacacc cccaggtctc gcggatggtc taggctgcgg gcccatggcc cgcatcctgc
      961 cgccgccggc cctgcccaac gctcctggcg agctgcgcca ggacccgctc agcggcgggt
     1021 gggtggcgat cacggtgggt cgggcggcca ggccggaggc gttcctcggc gacgtgggca
     1081 ccccgcgggg gccgctcggc tgcccgttct gtcccggcaa cgagcacatg accccgcccg
     1141 aggtctgggc cgaccgcgac ccggggaccg agcccgacaa gcccggctgg catgtccggg
     1201 tcgtccccaa caagttcccg gcgttcgccg gccccccggc cgaagcacgc aacaacgggg
     1261 gcctgtaccg gtcggtccca acggccgggg tgcacgaggt ggtcatccac aaccccgacc
     1321 acgaggccac cctggccgat ctgccgccgg cggcggtggc gcgggcgatg gcggcctggc
     1381 gggggcgcct ggccgtccac cacgaccagg cgttcggggc ggtgctggtc atcgtcaacc
     1441 agggccgcac cgccggggcc tccctggagc acccgcacag ccaggtgttc gccaccgccg
     1501 gacggcccga ccgcgtcagg gccgaggtgg accggctggc cggggacgcc tgcgccgcct
     1561 gcgcgaccgt ggccgccgag cgcggcggcc ccaggctcgt cggcgccaac ggggacctgc
     1621 tcaccctctg cccgtgggcg tcggcggccc cgttcgaggc gctgctcctg cccgccggcc
     1681 accgcccccg gttcgacgag ggcggccacg acgacaccgc catggcggcc agcctccgcg
     1741 acctgctgcg acggttccgc ggggtcgccg gcgaccacgc cccctacaac ctggtgctgc
     1801 actcggcccc gccgggggtc gacgacttcc actggcacct gcacctgctc ccccgcctca
     1861 ccacctacgg cgggttcgag ctgggcacgg gcgtgatcat caacgtggtc gaccccgacc
     1921 gggccgccga cgcgctccgg tcggccgcta acggttcctg aaggtcgcct tgcccggccc
     1981 gttggcgacg aacgaggcca tgccctcggc ggcgtcctcg gtggcgaaca gcgagtggaa
     2041 ggcctggcgc tccagcagca gcccggcgtc gatgccggcc tcgaggccgc cgtcgatgac
     2101 ctgcttggcc gcccgcaggg ccaggcccgg gcccttggcg tagccggccg cggcctcgca
     2161 cgcccgctgg tagacctcgc cggccgggtg cacctggtcg accaggccca tcgcctggca
     2221 ctcggcggcg ccgtagaagc ggcccgagta gatcatctcc ttggcctttg ccggcccgac
     2281 cag
//
LOCUS       DASSWR010000412         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1259321, whole genome shotgun sequence.
ACCESSION   DASSWR010000412 DASSWR010000000
VERSION     DASSWR010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259321"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1263
                     /locus_tag="VFO47_06860"
     CDS             115..1263
                     /locus_tag="VFO47_06860"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET8788451.1"
                     /translation="MDFETRAVHVGSEPDPTTGAVIPPIYQTSTFAQEAVGTHQGYEY
                     ARTGNPTRANLEACLASLEGVAPGGPGGGLCFASGMAATTTVLQTLAPGDHLVLSADV
                     YGGTYRVAARLFEGWGLRLSVVDMTSLDQLKQAIRSETRLVWVETPTNPLLGVVDIAG
                     AAEAAHAAGALCAVDNTFATPFLQRPLELGADLVVHSSTKYLGGHSDVVGGALVTAVP
                     ELYERARFLQNAAGAVPGPMDCWLTQRGIKTLALRMRAHCHNAMRVAAFLAGRPEVAE
                     VRYPGLAGDPGHELAARQMAAFGGMVAFRPAGGAEAAQRLVAATEVFTLAESLGGVES
                     LIELPGEMTHASVAGTDAEVPADLVRLSVGVEHPDDLVADLARALDRM"
     gene            1260..1769
                     /locus_tag="VFO47_06865"
     CDS             1260..1769
                     /locus_tag="VFO47_06865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788452.1"
                     /translation="MSGPPARPAVTIREATLADVDALVDLLVAVAGEGRWIGTEAPVD
                     VERRRRRMVEDVERADAIVLVAEAGGATVGELGLRLAGYGVAELGMAVAAGWRGRGVG
                     SALLTDGIDRARAAGAHKVALQVWPHNAAAIALYERFGFQREGYLHRHYRRRSGELWD
                     AIIMGLRLD"
     gene            1794..>2277
                     /locus_tag="VFO47_06870"
     CDS             1794..>2277
                     /locus_tag="VFO47_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8788453.1"
                     /translation="MEPKPPTKPTLDGLERKWSDRWARSGVYRFDRTRSRVEVFSIDT
                     PPPTVSGSLHLGHAFSYTQTDVVARFQRMRGRAVFYPMGWDDNGLPTERRVQQHFGVR
                     CDPSLPYKPAFEAPEWPSRQPIAVSRPNFVELCSQLTMEDEQAFEAVFRRLGLSVDWS
                     L"
BASE COUNT          282 a          841 c          858 g          296 t
ORIGIN      
        1 gcgttcacgc tgctcccggc cgaccagggc tgacacgcga ggcggtcgag gcgggcctat
       61 gctggccggc gacattccgc gacccagcga ggggatggcg tgagcttcga ggagatggac
      121 ttcgagacca gggcggtgca cgtcgggtcg gaacccgacc cgacgaccgg ggcggtgatc
      181 ccgccgatct accagacgtc gaccttcgcc caggaggcgg tggggacgca ccagggatac
      241 gagtacgccc ggaccgggaa cccgacccgg gccaacctcg aggcgtgcct ggcgtcgctg
      301 gagggggtgg cgcccggcgg gccggggggc gggctgtgct tcgcgtcggg gatggcggcg
      361 accacgacgg tgctgcagac gctggccccc ggcgaccacc tggtgctgtc ggccgacgtg
      421 tacggcggca cctaccgggt cgcggcccgg ctgttcgagg ggtgggggct gcggctcagc
      481 gtggtcgaca tgaccagcct ggaccagctc aagcaggcca tccggtccga gacccggctg
      541 gtctgggtgg agacgccgac caacccgctg ctcggggtgg tcgacatcgc gggcgcggcc
      601 gaggccgccc acgccgccgg ggcgctgtgc gccgtcgaca acaccttcgc caccccgttc
      661 ctgcagcggc ccctcgagct gggggccgat ctggtcgtcc actcctcgac caagtacctg
      721 ggcggccact ccgacgtggt cggcggggcc ctggtcacgg ccgtgcccga gctgtacgag
      781 cgggcgcgat tcctccagaa cgccgccggg gcggtgcccg ggccgatgga ctgctggctg
      841 acccagcgcg ggatcaagac cctggccctg cgcatgcggg cccactgcca caacgccatg
      901 cgggtggccg cgttcctggc cggccggccc gaggtggccg aggtccgcta cccgggcctg
      961 gccggcgacc ccggccacga gctggccgcc cggcagatgg ccgctttcgg gggcatggtc
     1021 gccttccgcc cggccggcgg ggccgaggcg gcccagcggc tggtcgccgc caccgaggtg
     1081 ttcaccctgg ccgagagcct gggcggggtg gagagcctga tcgagctgcc cggcgagatg
     1141 acccatgcca gcgtggccgg caccgacgcc gaggtcccgg ccgacctggt gcggctgtcg
     1201 gtcggggtcg agcaccccga cgacctggtc gccgacctgg cccgggccct cgaccggatg
     1261 tgagcgggcc gccggcccgt ccggcggtca ccatccggga ggccaccctg gccgacgtcg
     1321 acgccctggt cgacctgctg gtcgccgtgg ccggcgaggg ccgctggatc ggcaccgagg
     1381 ccccggtcga cgtggagcgc cgccgccgcc gcatggtcga ggacgtcgag cgcgccgacg
     1441 cgatcgtcct ggtcgccgag gccggcgggg cgacggtggg cgagctcggc ctgcgcctgg
     1501 ccggctacgg cgtggccgag ctgggcatgg ccgtggccgc cggctggcgt ggccgcgggg
     1561 tcggcagcgc cctgctcacc gacgggatcg accgggcccg ggcggccggc gcccacaagg
     1621 tcgccctcca ggtctggccc cacaacgccg ccgccatcgc cctctacgag cggttcggct
     1681 tccagcgcga gggctacctg caccgccact accggcgccg ctccggcgag ctgtgggacg
     1741 cgatcatcat gggtctgcgg ctggactgac ggcgcgggta aggtaaccgc ctcatggagc
     1801 cgaagccgcc gaccaagccg accctggacg ggctcgagcg caagtggtcc gaccggtggg
     1861 cgcgcagcgg cgtctaccgt ttcgaccgca cccgctcccg ggtcgaggtg ttctcgatcg
     1921 acaccccgcc gccgacggtc agcggctcgc tgcacctggg ccacgccttc tcctacaccc
     1981 agaccgacgt ggtcgcccgg ttccagcgca tgcgcggccg ggcggtgttc tacccgatgg
     2041 gctgggacga caacggcctg ccgaccgagc gccgggtcca gcagcacttc ggggtccgct
     2101 gcgacccgtc gctgccctac aagccggcct tcgaggcgcc cgagtggccc tcccggcagc
     2161 cgatcgccgt gtcccggccc aacttcgtcg agctctgcag ccagctcacc atggaggacg
     2221 agcaggcctt cgaggccgtc ttccgccgcc tcgggctgtc ggtcgactgg tcgctga
//
LOCUS       DASSWR010000413         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1344729, whole genome shotgun sequence.
ACCESSION   DASSWR010000413 DASSWR010000000
VERSION     DASSWR010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344729"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1278)
                     /gene="nadA"
                     /locus_tag="VFO47_06875"
     CDS             complement(139..1278)
                     /gene="nadA"
                     /locus_tag="VFO47_06875"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET8788454.1"
                     /translation="MASSTVFLDLLGPQRASEKGTTCPGDLPPASDPELVERARAARA
                     ALGERAFVLGHHYQRDEVIAFADARGDSFRLAQQAAARPEAEFVVFCGVHFMAESADI
                     LTGDHQQVVLPDLAAGCSMADMATWAQVDEAWEILQEWVPGGVVPLTYMNSSAAIKAF
                     TGRQGGAICTSSNAAKAMAWAFERAERVLFLPDQHLGRNTALAMGLDAADCAVWDPHR
                     DDGGLDPDRARAAKVLLWRGHCSVHHRFLPEHVDRVRERIPGVRVLVHPECRHEVVAK
                     ADEVGSTERIIKVVEASPPGSAWAIGTELNLVHRLATEHPDKTIAFLDRTVCFCSTMN
                     RIDLPHLTWALEELVAGRVPNRVRVDEATRAEALVALERMLALPG"
     gene            complement(1372..>2107)
                     /locus_tag="VFO47_06880"
     CDS             complement(1372..>2107)
                     /locus_tag="VFO47_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET8788455.1"
                     /translation="LETTHGRVGRLLAGLAPADWDRNVPASPAWSAADVVAHLAESDR
                     GALAAARGDWTFSGSLDDWTAAGVAAHRGEPPEARLEAWEAAADALRWHLAALDGEGW
                     GARVPWVAGPISLKTLAVLRLNDAWHHGRDVAEATGARFEIDEPTLAFLADLAARTIP
                     GGLSRRGRAHPGAVILLRLGDGRWLLGGAGGERPDPDAAPDLVLEADPLAFVLRAAGR
                     TTTDPWRAEGDGALAADVAATLSSIS"
BASE COUNT          255 a          761 c          840 g          251 t
ORIGIN      
        1 tggaggaggt gctgggcggg acgccggtct ggctggaggc ggccaacgcc ttcgtcgcct
       61 ccgccaccgg gtcccagctc gaggagatcg cccggtcgcg gctggtcaag gcgatccacc
      121 ccaaccgccg gctggggcct agccgggcag ggcgagcatc cgctccaggg cgaccagggc
      181 ttcggccctg gtcgcttcgt cgacccggac ccggttgggg acccggccgg ccaccagctc
      241 ctccagcgcc caggtcaggt gggggaggtc gatgcggttc atggtcgagc agaagcagac
      301 cgtccggtcc aggaaggcga tggtcttgtc cgggtgctcg gtggccagcc ggtggaccag
      361 gttcagctcg gtgccgatgg cccaggccga cccgggcggc gacgcctcca ccaccttgat
      421 gatccgctcg gtcgagccga cctcgtcggc cttggcgacc acctcgtggc ggcactcggg
      481 gtggacgagc acgcgcacgc cggggatgcg ctcgcgcacc cggtcgacgt gctcgggcag
      541 gaagcggtgg tggaccgagc agtgcccgcg ccacagcagc accttggccg cccgggcccg
      601 gtccgggtcg aggccgccgt cgtcgcggtg cgggtcccag accgcgcagt cggcggcatc
      661 caggcccatg gccagggcgg tgttgcggcc caggtgctgg tcggggagga acagcacccg
      721 ctcggcccgc tcgaacgccc aggccatcgc cttggccgcg ttcgaggagg tgcagatggc
      781 cccgccctgg cggccggtga acgccttgat ggcggccgag gagttcatgt aggtcagcgg
      841 cacgaccccg ccgggcaccc attcctggag gatctcccag gcctcgtcga cctgggccca
      901 ggtcgccatg tcggccatgg agcagccggc ggccaggtcg ggcaggacga cctgctggtg
      961 gtcgccggtg aggatgtcgg ccgactcggc catgaagtgg accccgcaga agaccacgaa
     1021 ctcggcctcg ggccgggccg cggcctgctg ggcgaggcgg aaggagtcgc cgcgggcgtc
     1081 ggcgaaggcg atgacctcgt cgcgctggta gtggtggccg aggacgaacg cccgctcgcc
     1141 cagggcggcc cgggccgccc gggcccgctc gaccagctcg gggtcggacg ccggcggcag
     1201 gtcgcccggg caggtggtgc ccttctcgga cgcccgctgg gggccgagca ggtcgaggaa
     1261 cacggtgctg gaggccatcg gtcgctccgt cgccgggacc ccgtcgccgg gatcgtgaac
     1321 gcgcgcacat tctagcgggt gggtaagccc accgccgtcc ggtcgatcgg gtcagctgat
     1381 gctgctgagg gtggcggcca cgtcggcggc cagggcgccg tccccctcgg cccgccaggg
     1441 gtcggtggtg gtgcggccgg cggcgcgcag gacgaaggcc agggggtcgg cctcgagcac
     1501 caggtcgggg gcggcgtccg ggtcggggcg ctcgcccccg gccccgccga gcagccagcg
     1561 gccgtcgccc agccggagca ggatgaccgc gcccgggtgg gcccggcccc ggcgcgacag
     1621 accgccggga atggtccggg cggccaggtc ggccaggaag gccagggtcg gctcgtcgat
     1681 ctcgaaccgg gccccggtcg cctcggccac gtcgcggccg tggtgccagg cgtcgttgag
     1741 ccgcagcacg gcgagggtct tgagcgagat cgggcccgcc acccagggga cccgggcccc
     1801 ccatccctcg ccgtccaggg cggccaggtg ccagcggagc gcgtcggcgg ccgcctccca
     1861 ggcctccagc cgggcctcgg gcggctcgcc ccggtgggcg gcgaccccgg cggcggtcca
     1921 gtcgtcgagc gacccggaga acgtccagtc gccccgggcg gcggccaggg cgccccggtc
     1981 gctctcggcc aggtgggcga cgacgtcagc ggccgaccag gccgggctgg cgggcacgtt
     2041 gcggtcccag tcggccgggg ccagcccggc gagcagccgc ccgacccggc cgtgggtcgt
     2101 ctccagg
//
LOCUS       DASSWR010000414         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1399676, whole genome shotgun sequence.
ACCESSION   DASSWR010000414 DASSWR010000000
VERSION     DASSWR010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399676"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFO47_06885"
     CDS             <1..213
                     /locus_tag="VFO47_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8788456.1"
                     /translation="RDEIRTYLASVYAVRGPVELSIKRQVGADQTVLAEWTSAYTEGG
                     RRSTGVPGVSVVEIGRDGIVYHRDFG"
     gene            complement(305..535)
                     /locus_tag="VFO47_06890"
     CDS             complement(305..535)
                     /locus_tag="VFO47_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007444428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HET8788457.1"
                     /translation="MEVKLGVTYSPKELVVETDQSPDDVASLVEEAVAGKSKVLWLSD
                     TRGRRVGVPTEKLAYVEVGEEHPDKRVGFSSL"
     gene            875..1912
                     /locus_tag="VFO47_06895"
     CDS             875..1912
                     /locus_tag="VFO47_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET8788458.1"
                     /translation="MIGLLAGAVAGLAFGRWGRGGDGGGVGGRELDRRLAGISDSLDR
                     RLVDLDRRLYLGLDSVQDAQARAADTAAQVRERVAVVAGVAEQVLEQARGLSRLEDLL
                     RPPQARGALGELLLEQLLAQGLPHSAYRTQHVFRSGARVDAVLVVGEAMVPVDSKFPL
                     EAFARMSTAGDGDAARALHRRAFVRDARRHVDAIADKYICPDEGTYDFALCYLPSEAV
                     FYEFLREEPPGDSCWRYAVERRVFPASPTTLYAYLVSIGVGLKGLRVEENARRVLDAL
                     AALRGDLDGFRTHFELVGRHLGRARDRWQDAAHRLDRFDGRLAEAAGRATDLDRGLPE
                     PEGGTGLTGTG"
     gene            complement(1896..>2859)
                     /locus_tag="VFO47_06900"
     CDS             complement(1896..>2859)
                     /locus_tag="VFO47_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8788459.1"
                     /translation="ETGRRPVSAEWPGLLWMLLLVPAALAAYLLAQRRRSRYTVRFTN
                     LDLLALAALLVSLARPQAMAMVPKEQATIVLVMDVSGSMNATDVQPTRLVSSQRAAAG
                     FIEQLPEKFRVGIVSFASTAQTLTRPTTDRAAVYEAIGSLHAEGATAMGDGIERALDV
                     KRPPTPASTGSAARPAPPAQQGGANEAPLVVLLLSDGANTQGRTQPMEAADDAKELGV
                     PVFTIALGTERGMVDVPDETGNLRRIPVPPDKLTLQRIAETTGARFFAAPSGDDLKAI
                     YRELGSKIGFVKEKQEITVVFAATGLLFLVAGAAMSLVWFSRFP"
BASE COUNT          356 a         1030 c         1089 g          384 t
ORIGIN      
        1 cgggacgaga tccgcaccta cctggcctcg gtgtacgcgg tcaggggccc ggtcgagctg
       61 agcatcaagc gccaggtcgg cgccgaccag accgtcctcg ccgagtggac cagcgcctat
      121 accgaaggcg gccgccgctc caccggcgtc cccggtgtct cggtggtcga gatcggccgc
      181 gacggcatcg tctaccaccg cgacttcggc tagcccggca cgcaagacgg tgggggacgc
      241 gggtcgttcc tgagccgcac cttcgtgacc ccggcggcgc ggagccaccg gccggccacc
      301 ggccctacag cgagctgaac ccgacccgct tgtcggggtg ctcctcgccg acctcgacgt
      361 aggccagctt ctcggtcggc acgccgacgc ggcggcccct ggtgtcggac agccacagca
      421 ccttggactt gccggccacc gcctcctcga ccaggctggc gacgtcgtcg ggcgactggt
      481 cggtctcgac gaccagctcc ttggggctgt aggtgacccc gagcttgacc tccatatgct
      541 tctcgtcctc ctggtcgcct ggtcggccgg tgatgtgcgg gaggccgagc gtagcaccag
      601 gacgctcaag cccggcctgg ggcgtagcga atcccgaacg caggcaggca acgccccaca
      661 gcgaggaagc gaggcccatc cgtgccgctc accggcccca ccccggccct gctcggcgcc
      721 gccgccatgt ccggcccgag ccctcggccg tcctggccgc cgcggcccag aactgacttc
      781 gtccggcggg gtgttgtcgc accccgttgg tagcgtcccg gccaactcgg acccccagca
      841 gagagggctg gtcgcggtgg gttgggaggc ggtggtgatc ggcctgctgg ccggggccgt
      901 ggccgggctg gcgttcgggc gctgggggcg cgggggcgac ggcggcgggg tcggggggcg
      961 ggagctggac cggcggctgg ccgggatctc cgacagcctc gaccggcggc tggtcgacct
     1021 ggaccggcgg ctctacctgg ggctggacag cgtgcaggac gcccaggcgc gggcggccga
     1081 caccgccgcc caggtgcgcg agcgggtggc ggtggtcgcc ggggtggccg agcaggtcct
     1141 tgagcaggcc cgcgggttga gccggctgga ggaccttctg cgcccgcccc aggccagggg
     1201 cgccctcggg gagctgctgc tggagcagct cctggcccag gggctgcccc acagcgccta
     1261 ccggacccag cacgtgttcc gctcgggggc ccgggtggac gcggtcctgg tggtcggcga
     1321 ggccatggtc ccggtcgact ccaagttccc gctggaggcc ttcgcccgca tgagcacggc
     1381 cggcgacggc gacgcggcca gggcgctgca ccggcgggcg ttcgtgcgcg acgcccgccg
     1441 gcacgtcgac gccatcgccg acaagtacat ctgccctgac gagggcacct acgacttcgc
     1501 gctctgctac ctgccgtcgg aggcggtgtt ctacgagttc ctgcgcgagg agccgccggg
     1561 ggactcctgc tggcggtacg cggtcgagcg gcgggtgttc ccggcctcgc cgaccaccct
     1621 gtacgcctac ctggtgtcga tcggggtcgg gctcaagggg ctgcgggtcg aggagaacgc
     1681 ccgccgggtg ctggacgcgc tggccgccct ccgcggcgac ctggacgggt tccgcaccca
     1741 cttcgagctg gtcggccgcc acctcggccg ggcccgggac cgctggcagg acgccgccca
     1801 ccgtctcgac cgcttcgacg gccgcctcgc cgaggcggcc ggccgcgcca ccgacctcga
     1861 ccgcgggctt ccggagcccg agggggggac cggcctcacg ggaaccggct gaaccagacc
     1921 agggacatgg cggcgccggc gaccaggaag agcaggccgg tggcggcgaa gaccaccgtg
     1981 atctcctgct tctccttgac gaagccgatc ttggagccca gctcgcggta gatggccttg
     2041 aggtcgtcgc cgctgggggc ggcgaagaac cgggccccgg tggtctcggc gatccgctgc
     2101 agggtgagct tgtccggcgg gaccgggatg cggcgcaggt tgccggtctc gtcgggcacg
     2161 tcgaccatcc cacgctcggt cccgagggcg atcgtgaaca ccggcacccc gagctccttg
     2221 gcgtcgtcgg cggcctccat cggctgggtc cggccctggg tgttggcccc gtccgacagc
     2281 agcagcacga ccagcggggc ctcgttggcg ccgccctgct gggccggcgg ggccggccgg
     2341 gccgcgctgc cggtcgaggc cggggtcggg ggccgcttga cgtcgagggc ccgctcgatc
     2401 ccgtccccca tggccgtcgc cccctcggcg tgcagcgacc cgatcgcctc gtagaccgcg
     2461 gcccggtcgg tggtcggccg ggtcagggtc tgggcggtgg aggcgaacga cacgatcccg
     2521 acccggaact tctccgggag ctgctcgatg aagccggccg cggcccgctg ggacgacacc
     2581 agccgggtcg gctgcacgtc ggtggcgttc atcgagccgg acacgtccat gaccaggacg
     2641 atggtggcct gctccttggg caccatggcc atcgcctggg gccgggccag gctgaccagc
     2701 agggcggcca gggccagcag gtcgaggttg gtgaaacgga cggtgtagcg ggagcggcgt
     2761 cgctgggcga gcaggtaggc ggcgagagcg gccgggacga gcaggagcat ccagagcagg
     2821 cccggccact ccgcgctcac cggacgccgc cccgtttca
//
LOCUS       DASSWR010000415         6460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_14294,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000415 DASSWR010000000
VERSION     DASSWR010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14294"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFO47_06905"
     CDS             <1..868
                     /locus_tag="VFO47_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788460.1"
                     /translation="YDEARAERARLEEAARQKREWAQSRRGERRTDNDKALAGRRKER
                     ATAGATRAKAIERRLERLGDPEKPWEGWDLRLSLQAGRRSGEVVASLAGAVVERGRFR
                     LGPVDLELRWRDRLALTGPNGAGKSTLLGLLTGELRPAAGTVRLGSGVVVGHLGQDRA
                     PGEGTLLDAVRERTELPVEEARSLLAKFDLGAEHLSRPEAALSPGERSRAGLAVLVAL
                     GTNLLLLDEPTNHLDLDAIEELERALGGYDGTLVVVSHDRRLLANLRLDRRLAVVAGT
                     VAEAPLPVAAPG"
     gene            879..1610
                     /gene="recO"
                     /locus_tag="VFO47_06910"
     CDS             879..1610
                     /gene="recO"
                     /locus_tag="VFO47_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET8788461.1"
                     /translation="MNLYREQGVVLRTWKLGEADRIVVFLTQGEGKVRAVAKGVRKTK
                     SRFGGRLEPFSHVDLSLYRGRELDIVTQAEVISPFRALREDYDRVVAGTAMLEAVDQV
                     AQERETAIRLYLLLVRALRALDGGPRDLSVVLDAFLLKLMALEGYRPALAECAGCGAK
                     EQPPRCFSIARGGGLCERCRTGEESNLDAATMPLLAALLGDDLDTTGQRDPAPVSRRE
                     AGALVKGYVEYHLDRRLRAYPLVAR"
     gene            1610..2401
                     /gene="uppS"
                     /locus_tag="VFO47_06915"
     CDS             1610..2401
                     /gene="uppS"
                     /locus_tag="VFO47_06915"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET8788462.1"
                     /translation="MPSRNPRALPPGLDPKAVPDHVAIVMDGNGRWAGAKGLPRNKGH
                     EAGEAALFDTVEGALDAGLSWLTVYAFSTENWRRPASEVRFLMNFNESLLVRRSHELH
                     EREVRIRFIGRRDRIPGHVRRRIEEAEGLTAGDTRMTLAVALDYGGRDELARAVQALA
                     AEVREGRGPRRIDERAIARHLYASDMPDVDLLIRTSNEYRISNFLLWQIAYAELHFTP
                     VLWPDFNRQELFDALRVYQGRHRRFGAVADPAGRPRVEPTSAPPR"
     gene            2398..3219
                     /locus_tag="VFO47_06920"
     CDS             2398..3219
                     /locus_tag="VFO47_06920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788463.1"
                     /translation="MIRPARPQDLARLGPLYEQAGFGARLAGVVGFARARLDGEVVVA
                     EAGDELAGVAAGAVFAGTGWVGGVAVAPAHRRAGLGGALTEAIIGFLEERGVTSVLLH
                     ATALGRPVYERLGFVPETAYVTLSGPTLARASGAAPGIRAGRAADLEAVLAIDRAVTG
                     EDRRRLLTALWPAGGLVAARDGRPLGYHLASPWRSGGATVAADPGTGLALLDAVRVAP
                     GNQVALSVPAANTAAVEALQSAGFAERYRTVRMHRGPRVAWDPAALFTAHNLFWG"
     gene            complement(3270..3797)
                     /locus_tag="VFO47_06925"
     CDS             complement(3270..3797)
                     /locus_tag="VFO47_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788464.1"
                     /translation="MRTMAKAVVLLLVVAFVAGVWDARYGNDQPPASSGPPFRTAPVE
                     RNRASRPAPELVSVATSERDGYDRVLFTFRGAAPGYQVRYVSRVTDQAGRRLALDGQA
                     FLAVVFEPARARDPGGQATFASGDLEPGSPALRQVRFAGDFEGQVSFGIGVADRGGFR
                     VSELRDPARVAVDVR"
     gene            3877..4830
                     /gene="deoC"
                     /locus_tag="VFO47_06930"
     CDS             3877..4830
                     /gene="deoC"
                     /locus_tag="VFO47_06930"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET8788465.1"
                     /translation="MATTPVRLAEAGSGFLQQVALGPLLSRFAVDQVGAEERAAALAK
                     RSIKKDSKVAALRLAVAVMDLTTLEGSDTVGKVEALCSKARRPDPSDPEVPPVAAVCV
                     YPSLVATAKAALAGSGVKVASVATAFPSGQASLAVRLADVAEAVEAGADEVDMVINRG
                     AFLAGRHQEVFEEIVVVKEACGQAHLKVILETGELGTLDNVRRASVLAMAAGADFIKT
                     STGKIQPAATLPVALVMLEAIRDFHDRTGRMVGMKPAGGVRTAKQAIQHLVVVNETLG
                     PDWLTPDWFRLGASSLLNDCLMQLAKERTGRYQSGDYFTKD"
     gene            4887..6365
                     /locus_tag="VFO47_06935"
                     /pseudo
     CDS             4887..6365
                     /locus_tag="VFO47_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT          835 a         2480 c         2355 g          790 t
ORIGIN      
        1 ctacgacgag gccagggccg agcgggcccg gctggaggag gcggcccgtc agaagcgcga
       61 gtgggcgcag agccgccggg gcgagcgccg caccgacaac gacaaggccc tggccggccg
      121 gcgcaaggag cgggccaccg ccggggcgac cagggccaag gcgatcgagc ggcgcctgga
      181 gcgcctgggc gaccccgaga agccgtggga gggctgggac ctgcgcctgt cgctgcaggc
      241 cggccggcgc agcggcgagg tcgtggccag cctggccggg gccgtggtcg agcggggccg
      301 cttccggctg ggcccggtcg acctggagct gcgctggcgc gaccgcctcg ccctgaccgg
      361 ccccaacggc gccggcaagt cgaccctgct cggcctgctg accggtgagc tgcgcccggc
      421 cgcgggcacg gtccggctgg ggtcgggggt ggtcgtcggc cacctcggcc aggaccgcgc
      481 cccgggcgag gggacgctgc tggacgcggt ccgggagcgg accgagctgc cggtcgagga
      541 ggcgcggtcg ctgctggcca agttcgacct gggcgccgag cacctgagca ggcccgaggc
      601 ggcgctgtcg ccgggcgagc gctcccgggc cggcctggcc gtgctggtcg ccctgggcac
      661 caacctcctg ctgctcgacg agcccaccaa ccacctcgac ctggacgcca tcgaggagct
      721 ggaacgggcc ctggggggct acgacggcac cctggtggtg gtcagccacg accggcggct
      781 gctggccaac ctgcgcctgg accgacgcct tgcggtggtc gcgggcacgg tcgccgaggc
      841 acccctgccc gtcgccgcgc ccggatagac tgggcggaat gaacctctac cgcgagcagg
      901 gcgtcgtgct gcggacctgg aagctcggcg aggccgaccg catcgtcgtg ttcctcaccc
      961 agggcgaggg caaggtcagg gcggtcgcca agggggtgcg caagaccaag tcgcgctttg
     1021 gcggccgcct ggagccgttc agccacgtcg acctgtcgct gtaccggggg cgcgagctcg
     1081 acatcgtcac ccaggccgag gtcatcagcc cgttccgggc cctgcgggag gactacgacc
     1141 gggtggtcgc cggcacggcc atgctggagg cggtcgacca ggtcgcccag gagcgcgaga
     1201 ccgccatccg gctgtacctg ctgctggtcc gggccctgcg ggccctggac ggcggccccc
     1261 gcgacctgtc ggtggtgctc gacgccttcc tgctcaagct gatggccctt gagggctacc
     1321 ggccggcgct ggccgagtgc gccggctgcg gggccaagga gcagccgccg cgctgcttct
     1381 ccatcgcccg cggcggcggg ctgtgcgagc gctgccgcac cggcgaggag tcgaacctgg
     1441 acgccgccac catgccgctg ctggccgccc tgctcggcga cgacctggac accaccgggc
     1501 agcgcgatcc cgcccccgtg tcccggcgcg aggccggcgc cctggtcaag ggctacgtcg
     1561 agtaccacct ggaccggcgc ctgcgcgcct acccgctggt ggcccgctga tgccgagccg
     1621 caacccgcgg gcgctgccgc ccggcctcga ccccaaggcc gtgcccgacc acgtggccat
     1681 cgtcatggac ggcaacggcc gctgggccgg ggccaagggg ctgccccgca acaagggcca
     1741 cgaggcgggg gaggcggccc tgttcgacac cgtcgagggg gcccttgacg ccgggctgtc
     1801 ctggctgacc gtgtacgcct tctccaccga gaactggcgc cggccggcct ccgaggtccg
     1861 gttcctgatg aacttcaacg agtcgctgct ggtccgccgc tcccacgagc tgcacgagcg
     1921 cgaggtccgg atccgcttca tcggccggcg cgaccgcatc cccggccacg tccgccgccg
     1981 catcgaggag gccgagggcc tcaccgccgg cgacacccgc atgaccctgg ccgtcgccct
     2041 cgactacggc ggccgcgacg agctggccag ggccgtgcag gccctggccg ccgaggtccg
     2101 cgagggccgc ggcccccgcc gcatcgacga gcgggccatc gcccggcacc tctacgcgtc
     2161 cgacatgccg gatgtcgacc tgctcatccg cacctccaac gagtaccgga tctccaactt
     2221 cctgctctgg cagatcgcct acgccgagct ccacttcacc ccggtgctgt ggcccgactt
     2281 caaccgccag gagctgttcg acgccctccg ggtctaccag ggccgccacc gccgcttcgg
     2341 tgccgtggcc gaccccgccg gcaggccccg ggtcgagccc accagcgccc ctccgcgttg
     2401 atccggccgg cccggcccca ggacctggcc cggctggggc cgctgtacga gcaggccggg
     2461 ttcggagccc ggctggccgg ggtggtgggg ttcgcccggg cccggctgga cggcgaggtc
     2521 gtggtcgccg aggccgggga cgagctggcc ggggtggccg ccggggcggt gttcgccggc
     2581 accggctggg tcgggggcgt ggccgtggcc ccggcgcatc gccgggccgg gctgggcggc
     2641 gccctcaccg aggccatcat cgggttcctg gaggagcggg gcgtcaccag cgtgctgctg
     2701 cacgccaccg ccctggggcg gcccgtctac gagcgcctcg gcttcgtccc cgagaccgcg
     2761 tatgtgaccc tcagcggccc caccctggcc cgcgcctccg gggcggcgcc ggggatccgg
     2821 gccggacggg cggccgacct ggaggcggtg ctggccatcg accgggccgt caccggcgag
     2881 gaccgccggc ggctgctgac cgccctgtgg ccggccggcg gcctggtggc ggcacgcgac
     2941 ggccggccgc tcggctacca cctggcctcg ccgtggcggt cgggcggggc caccgtcgcc
     3001 gccgaccccg gaaccggcct ggccctcctg gacgccgtcc gggtcgcgcc cgggaaccag
     3061 gtcgcgctct cggtgcccgc cgccaacacc gcggccgtcg aggcccttca gtcggccggc
     3121 ttcgccgagc gctaccgcac cgtccggatg caccgcggcc cccgggtcgc ctgggacccg
     3181 gccgccctgt tcaccgccca caacctgttc tggggctgac cctggaaccg gccgctcctc
     3241 tgggggattc ccggggccgg atctgcgtcc tagcggacgt cgaccgcgac ccgggccggg
     3301 tcgcggagct cggagacccg gaagccgccg cggtcggcca cgccgatccc gaaggacacc
     3361 tggccctcga agtcgccggc gaagcgcacc tggcgcaggg ccggggagcc gggctcgagg
     3421 tcgccgctgg cgaaggtggc ctggccgccg gggtcgcggg cccgggccgg ctcgaacacc
     3481 acggccagga acgcctgccc gtccagggcc agcctccggc cggcctggtc ggtgacccgg
     3541 gacacgtagc ggacctggta gccgggggcc gcgccccgga aggtgaacag cacccggtcg
     3601 tacccgtccc gttcgctggt cgcgaccgag accagctccg gcgccgggcg gctggcccgg
     3661 ttccgctcga ccggcgccgt ccggaacggc ggccccgagc tcgccggcgg ctggtcgttc
     3721 ccgtagcgcg cgtcccacac cccggccacg aaggccacga ccagcagcag gacgaccgcc
     3781 ttggccatgg tccgcatcgt cgggttcctc cccgggcgcc ggtctccgtc cctccggcac
     3841 agatggtgac agccccgccg ctacaatgag gcgaccatgg ccaccacccc cgtgcggctc
     3901 gccgaggccg gcagcggctt cctgcagcag gtcgccctcg gcccgctgct cagccgcttc
     3961 gccgtcgacc aggtgggggc cgaggagcgg gccgccgccc tggccaagcg gagcatcaag
     4021 aaggactcca aggtcgccgc cctgcgcctg gccgttgccg tcatggacct gaccaccctc
     4081 gaggggtcgg acacggtcgg caaggtcgag gccctctgct ccaaggcccg ccgccccgac
     4141 ccgtccgacc ccgaggtccc gccggtggcc gccgtctgcg tctacccgtc gctggtcgcc
     4201 accgccaagg cggcccttgc cgggtcgggg gtcaaggtcg cctcggtcgc caccgccttc
     4261 cccagcggcc aggcgtcgct ggcggtgcgc ctggccgacg tggccgaggc cgttgaggcc
     4321 ggcgccgacg aggtcgacat ggtgatcaac cggggcgcct tcctggccgg ccgccaccag
     4381 gaggtgttcg aggagatcgt ggtcgtcaag gaggcctgcg gccaggccca tctcaaggtg
     4441 atcctggaga cgggcgagct cggcaccctc gacaacgtcc gccgggccag cgtcctggcc
     4501 atggccgccg gggccgactt catcaagacc tccaccggca agatccagcc ggccgccacc
     4561 ctcccggtgg ccctggtcat gctggaggcg atccgcgact tccacgaccg caccggccgc
     4621 atggtcggca tgaagccggc cggcggcgtc cgcaccgcca agcaggccat ccagcacctg
     4681 gtggtggtca acgagaccct cggccccgac tggctcaccc ccgactggtt ccgcctcggc
     4741 gcctccagcc tcctcaacga ctgcctcatg cagctggcca aggaacgcac cggccgctac
     4801 cagtcgggcg actacttcac caaggactag gggttcgggg ggctcaagga gcgaagcggt
     4861 agccccccga tcaacaaggg agcgccatgg cagagcagct ggaacggcgg accgatcggg
     4921 cgccggcccg gttcgggggg ctcgactacg cgcccgcgcc cgaggcgacc gaccacgtca
     4981 agctgcgggc cgatcacggg ctgctgatcg gcgggcggtg ggtcgagccg ctgtccgagc
     5041 ggcgcttcaa gaccatcaac ccggccaccg aggaggtcct ggccgaggtc tccgaggccg
     5101 gcgaggagga cgtggacgcg gccgtcaagg cggcccgggc cgcctacacc aaggtgtggt
     5161 cgcggatgcc gggggcggag cgggccaagt acctctaccg gatcgcgcgg gtcctgcagg
     5221 aacgggcgcg ggagttcgcc gtgctggaga ccatgaacgg cggcaagccg atcaaggagt
     5281 cccgcgacgt cgacgtgccc ctggccgcgg cccacttctt ctaccacgcc ggctgggccg
     5341 acaagctcga ctacgccttc cccggccgcc gcccggcccc cctcggggtg gccgcccagg
     5401 tcatcccctg gaacttcccg ctgctgatgg ccgcctggaa gctcgccccg gccctggccg
     5461 ccggcaacac cgtggtcctc aagccggccg agacgacccc gctcaccgcc ctgcgcctgg
     5521 gcgaggtcct cctggaggcc gagctgcccg agggcgtggt caacatcctc accggcgccg
     5581 gcgcgaccgg cgcggccctg gtcggccacc ccggcgtgga caaggtcgcc ttcaccggct
     5641 ccaccgaggt cggcaagcag atccagagga ccctggccgg gtccggcaag aagctcaccc
     5701 tggagctggg cggcaaggcg gccaacatcg tcttcgagga cgcccccctc gaccaggcgg
     5761 tcgaggggat cgtcaacggc atctacttca accagggcca cgtctgctgc gccgggtcgc
     5821 ggctgctggt ccaggagtcg atcgccgagc cgctggtcga caagctcaag cggcgcatgg
     5881 cgaccctgcg ggtcggcgac ccgctggaca agaacaccga cgtcggggcg atcaactccc
     5941 gggcccagct ggacaagatc accgagctgg tcgccgccgg ggaggccgac ggcgcccagg
     6001 cctggcaggc gccgtgcgag ctgccctcgc gggggttctg gttccggccg accgtgttca
     6061 cctccgtcgc ccagtcgtac cggatcgccc aggaggagat cttcgggccg gtgctgtcgg
     6121 tgctgacctt ccgcaccccc gacgaggcgg tggagaaggc caacaacacc ccctacgggc
     6181 tgtcggccgg ggtctggacc gacaagggca gccgcatcct cgacctggcc aaccgcatgc
     6241 gcgccggggt cgtatgggcc aacaccttca accgcttcga ccccacctcg ccgttcggcg
     6301 gctaccgcga gtccggcttc ggccgcgagg gcggccgcca cggcctggcc gcctacctcg
     6361 acctgtcctc gggcagcggg caggagccgt ccccagccgc cccgcgtgca ggcccacgtg
     6421 ggccgggggc aggccggcct gcggggtccg ctccaccatc
//
LOCUS       DASSWR010000416         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_185842,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000416 DASSWR010000000
VERSION     DASSWR010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185842"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFO47_06940"
     CDS             complement(<1..680)
                     /locus_tag="VFO47_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788466.1"
                     /translation="MRHPPDLPDGTVVTAVRGGFGVETATLVPLPVGNDADSWSYRVE
                     TAAGPTWFLKVRAGPSPGRGAAVPALLQRRGVPNVLAPLAAAGPGDPVAFELAGFWRD
                     RRDRIATLVHRADRLGRELAGEPFPLVLCHADLHTWNVLVGACCPGPSKVMAWWPRSS
                     AANPPGLASGARKKYPGRDVPSYGIWSRSPGTAARSTASSQHARCRFQSSSCRSGALE
                     CAIRYSPV"
     gene            740..1534
                     /locus_tag="VFO47_06945"
     CDS             740..1534
                     /locus_tag="VFO47_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8788467.1"
                     /translation="MSPDRQPAVALDRGGAMPLVGLGTWQMTGGRCQAAVRSALEAGY
                     RHLDTATIYRNEREVGRAVRDSGVPREQVFVTTKLPPGNAGRERRTLTESLRALGMDH
                     VDLWLVHWPPRARALVPTWEALLAARDQGLARAVGVSNYSPAQLDELIDATGQAPQVN
                     QIPWAPALHDAALLDAHRRRGVVLEGYSPFKNTDLRHPVLADIAARHGVTPAQVVVRW
                     HIDHEVVVIPKSADPGRIATNFDVFGFSLTDEERGRIDGLSATRRR"
     gene            1608..2213
                     /locus_tag="VFO47_06950"
     CDS             1608..2213
                     /locus_tag="VFO47_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET8788468.1"
                     /translation="MREITVFNSVTLDGVTQAPGRPDEDTRGGFANGGWAAPYMDEVM
                     GRVAAEGMAGPGALLLGRRTYEDFHGFWPHQTDNPFTEVLDSTRKYVASTTLREPLPW
                     RNSTLLEGDAAEAVARLKEQPGEDLTVLGSGELVQSLRRRDLVDRYVLLIHPLVLGSG
                     RRLFPDGGPASTLRLVGSVPTTTGVIIATYQPVTSREGAAR"
     gene            2210..2563
                     /locus_tag="VFO47_06955"
     CDS             2210..2563
                     /locus_tag="VFO47_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005255108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8788469.1"
                     /translation="MKQYLLTVYQPDGPPPPAEALEAIFRDLDALNEEMRAAGAWVFA
                     GGLHPPSTATVIRVSGGETLVTDGPFAEGKEHVGGFTIVKAPDLDAALEWGRRLAQVT
                     TLPIEVRPFQDEAGD"
     gene            2571..>3038
                     /locus_tag="VFO47_06960"
     CDS             2571..>3038
                     /locus_tag="VFO47_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8788470.1"
                     /translation="MPTLPTSEIEGVFREHYGRAVAVLVGAFGDIDLAEEAVQEAFAT
                     AVARWPSTGPPPSPPGWIITTARNKAVDRLRREASREDRHAQAALLQARDEPAEEGAV
                     RDDRLRLIFTCCHPALGTGAQVALTLRLLGGLTTAEIARAFLVPEATLAQRLVR"
BASE COUNT          401 a         1126 c         1140 g          371 t
ORIGIN      
        1 tcgaccgggc tgtacctgat cgcgcactcg agcgcgcccg agcggcagct cgagctctgg
       61 aagcggcacc gggcgtgctg ggacgaggcg gtcgacctgg cggcggtgcc gggggaacgg
      121 ctccagatcc cgtacgaggg cacgtccctg cccgggtact tcttccgggc gcccgatgcc
      181 aggcccggcg ggttggcggc gctggagcgc ggccaccacg ccatgacctt cgacgggcca
      241 gggcagcagg cgccgaccag cacgttccag gtgtgcaggt cggcgtggca gagcaccagc
      301 gggaacggct cgccggcgag ctcgcgtccc agccggtcgg ctcgatgcac cagggtggcg
      361 atgcggtcgc gccgatcccg ccagaacccg gccagctcga aggccaccgg gtcgccgggg
      421 ccggcggcgg ccagcggggc caggacgttc ggcaccccgc ggcgctgaag gagggcgggg
      481 acggccgccc cgcgtccggg gctggggccg gcccggacct tgaggaacca ggtcgggccg
      541 gccgccgtct cgacccggta ggaccaggag tcggcgtcgt tgccgaccgg gagcgggacc
      601 agcgtggcag tctcgacccc gaagccgccg cgcacggcgg tgacgacggt gccgtcgggc
      661 aggtcgggcg ggtgcctcat ccgcctggac tctaccgggc cggctcgacg ttctcgtgcc
      721 ggtgggcgac gatgtcgtca tgtcccctga ccgccagccc gccgtggccc tcgaccgggg
      781 cggggccatg ccgctggtcg ggctcggcac ctggcagatg acgggcgggc gctgccaggc
      841 ggccgtccgc tcggcgctgg aggccggcta ccgccacctc gacacggcca ccatctaccg
      901 caacgaacgc gaggtgggcc gggcggtccg cgacagcggg gtcccgcgcg agcaggtgtt
      961 cgtcaccacc aagctgccgc cgggcaacgc cggccgggag cggcgcacgc tcaccgagag
     1021 ccttcgggcg ctgggcatgg accatgtgga cctgtggctg gtccactggc cgccccgggc
     1081 ccgggccctg gtgccgacct gggaggcgct cctggccgcg cgcgaccagg ggctggcccg
     1141 cgccgtcggg gtcagcaact acagcccggc ccagctggac gagctgatcg acgccaccgg
     1201 ccaggccccc caggtcaacc agatcccgtg ggcgcccgcc ctgcacgacg ccgccctgct
     1261 cgacgcccac cgccggcgcg gggtcgtcct tgaggggtac agcccgttca agaacaccga
     1321 cctgcgccac cccgtgctgg ccgacatcgc cgcccgccac ggggtgaccc cggcccaggt
     1381 cgtggtgcgc tggcacatcg accacgaggt ggtggtgatc cccaagtcgg ccgaccccgg
     1441 gcggatcgcc accaacttcg acgtcttcgg cttctcgctc accgacgagg agcggggtcg
     1501 catcgacggc ctgtcggcca cccgccgccg gtaggtgtcg atccgggggg cacccgttcg
     1561 tgtagagggt gagagattcc cgacgcgaac ggacggaggg accgcggatg cgcgagatca
     1621 ccgtgttcaa cagcgtgacg ctggacggcg tgacgcaggc ccccgggcgg cccgacgagg
     1681 acacccgtgg cgggttcgcc aacggcggct gggcggcgcc gtacatggac gaggtcatgg
     1741 gccgggtcgc cgccgagggc atggccggcc ccggggccct gctgctcggg cggcggacct
     1801 acgaggactt ccacggcttc tggccccacc agaccgacaa ccccttcacc gaggtgctgg
     1861 acagcacccg caagtacgtc gcgtccacga cgctgcggga gccgctcccc tggcgcaact
     1921 ccaccctgct ggagggcgac gccgccgagg ccgtggcccg gctgaaggag cagccgggcg
     1981 aggacctgac ggtgctgggc agcggcgagc tggtccagtc gctgcggcgg cgcgatctgg
     2041 tcgaccggta cgtcctcctg atccaccccc tcgtgctggg gtcggggcgg cggctgttcc
     2101 ccgacggcgg gccggcgtcg acgctgcggc tggtcggctc ggtgccaacg acgaccggtg
     2161 tcatcatcgc cacctaccag ccggtgacct ccagggaagg agcggcccga tgaagcagta
     2221 cctgctcacc gtgtaccagc ctgacgggcc gccgccgccg gccgaggccc ttgaggcgat
     2281 cttcagggac ctcgacgccc tcaacgagga gatgcgggcg gccggcgcct gggtgttcgc
     2341 ggggggcctg cacccgccga gcacggccac ggtgatccgg gtctccggcg gggagacgct
     2401 ggtcaccgac gggcccttcg ccgagggcaa ggagcacgtc ggcgggttca ccatcgtcaa
     2461 ggcccccgac ctcgacgccg ccctcgagtg gggccgcagg ctcgcccagg tgaccaccct
     2521 gccgatcgag gtgcggccgt tccaggatga ggccggggac tgagcctggc gtgccgacgc
     2581 tccccacctc ggagatcgaa ggtgtcttcc gcgagcacta cggccgcgcc gtcgccgttc
     2641 tggtcggcgc cttcggggac atcgacctgg ccgaggaggc ggtccaggag gccttcgcca
     2701 cggccgtggc ccggtggccg tccaccgggc cgccgccgag cccgccggga tggatcatca
     2761 ccaccgcccg caacaaggcc gtcgaccgcc tgcgccgcga ggcctcccgc gaggaccggc
     2821 acgcccaggc ggccctgctg caggcccggg acgagccggc cgaggagggc gccgtgcgcg
     2881 acgaccggct gcggctgatc ttcacctgtt gccaccccgc cctcggcacc ggcgcccagg
     2941 tggcgctgac cctgcggctg ctgggcgggc tcaccaccgc cgagatcgcg cgcgcgtttc
     3001 tcgtgccgga ggcgacgctc gcgcagcgtc tggtgcgc
//
LOCUS       DASSWR010000417         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1344821, whole genome shotgun sequence.
ACCESSION   DASSWR010000417 DASSWR010000000
VERSION     DASSWR010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344821"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFO47_06965"
     CDS             complement(<1..1031)
                     /locus_tag="VFO47_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET8788471.1"
                     /translation="MSATAPAVPQGRLLRRGLRVIRRQVALHPRPFAIAVLGAAVYAL
                     GTVAQSWVLGQVVARAVTPRFESGQLETGVAVAAAAAIVAVGVIKTAGIITRRIAATI
                     VHANVQATLRDRLAERYHRLPLTWHHEHPAGELLSRAEGDLDAASEILSPLPFATGVL
                     TMLAATVVWLFLADPFLATLGLLVVPAILVLNLAFQRRVEHPTTLGQERLGDMSAVAH
                     ESFDGALVVKALQAEEAEGRRFADKAELLRDARIEAARVTANFEASLDALPAVTTVAL
                     LVIGVWRIEQGAITPGTLVGLINLFALLIWPLRLIGYVLGDMPRAVAGYERVAEVLAE
                     PLPAARPAPA"
     gene            complement(1028..2434)
                     /locus_tag="VFO47_06970"
     CDS             complement(1028..2434)
                     /locus_tag="VFO47_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8788472.1"
                     /translation="MSGTAGAPPELEALFAHVDANRAAFVERLAGWVRVPSVSATGQG
                     MPEAAGHARDLVAAAGLDATVVSTAGWPLVLGHRAGPPGAPTVLVYGHYDVQPPDPLD
                     AWTSPPFEPEVRDGRLYGRGGADNKGQHLAQLLAVESLLATRGELPCTVKVLLDGEEE
                     IGSPNLAAFAAGQRGRLAADLVVWSDGPVDPGGGWRLVFGVRGIASFELRARGANRSL
                     HSGNWGGVVPNPLWTLVQLLATMRDAQGRITVAGFADEVEPLGEAERQALARLPVDVE
                     AVKAELGLAELDAPVERGFAERLAAWPTLTINGLQGGYGGPGTQTVLPSEAVAKCDVR
                     LVHAQRAADVYDKLEAHVRRHTPGVELVRQGSMEPSRTPMRSPFTGPIRAGMAAAQGA
                     EPLLVPTLGGSLPLHVFTGVLGLPTFGIPLGNPDQANHAPNENLDLERFHTGIKTAAA
                     VLTKLGSFRPAGSGTMAG"
BASE COUNT          317 a          904 c          992 g          297 t
ORIGIN      
        1 gccggcgccg gacgggccgc cggcagcggc tcggccagga cctcggccac ccgctcgtag
       61 ccggcgacgg cccggggcat gtccccgagc acgtagccga tcaggcgcag cggccagatc
      121 agcagggcga acaggttgat caggccgacc agggtgccgg gggtgatcgc cccctgctcg
      181 atccgccaga cgccgatgac cagcagggcg accgtggtca cggcgggcag ggcgtcgagg
      241 ctggcctcga agttggccgt cacccgggcg gcctcgatgc gggcgtcgcg cagcagctcc
      301 gccttgtcgg caaagcgccg gccctcggcc tcctcggcct ggagggcctt gaccaccagg
      361 gcgccgtcga agctctcgtg ggccaccgcc gacatgtcgc cgaggcgctc ctggcccagg
      421 gtggtcgggt gctcgacccg ccgctggaag gccaggttga ggacgaggat ggccgggacc
      481 acgagcaggc cgagggtggc caggaacggg tcggccagga acagccagac cacggtggcc
      541 gcgagcatgg tcagcacccc ggtggcgaac ggcaggggcg agaggatctc ggaggcggcg
      601 tccaggtcgc cctcggcccg cgacagcagc tcgcccgccg ggtgctcgtg gtgccaggtc
      661 agcgggaggc ggtggtagcg ctcggccagc cggtcgcgca gggttgcctg gacgttcgcg
      721 tggacgatgg tggcggcgat gcggcgggtg atgatgccgg ccgtcttgat caccccgacg
      781 gccacgatgg cggcggccgc ggccaccgcc acgccggtct ccagctggcc gctctcgaac
      841 cggggcgtga ccgcccgggc gaccacctgg ccgagcaccc acgactgggc taccgtcccg
      901 agggcgtaga cggccgcccc gagcacggcg atggcgaacg gccgggggtg cagggcgacc
      961 tggcggcgga tcacgcgcag gccgcgccgg agcaggcgtc cctggggaac ggcgggggct
     1021 gtggcgctca cccggccatg gtgccggaac ccgccggccg gaaggaaccg agtttcgtga
     1081 gcacggccgc ggccgtcttg atcccggtgt ggaagcgctc caggtcgagg ttctcgttgg
     1141 gggcgtggtt ggcctggtcg gggttgccga gggggatccc gaaggtgggc aggccgagca
     1201 ccccggtgaa cacgtgcagc gggaggctgc ccccgagggt gggcacgagc agcggctcgg
     1261 ctccctgggc ggcggccatg ccggcccgga tggggccggt gaagggggag cgcatcgggg
     1321 tccgcgacgg ctccatcgag ccctggcgga cgagctcgac cccgggggtg tgccggcgga
     1381 cgtgggcctc gagcttgtcg tagacgtcgg ccgcccgctg ggcgtggacc aggcgcacgt
     1441 cgcacttggc cacggcctcc gacggcagca cggtctgcgt gccggggccg ccgtagccgc
     1501 cctggaggcc gttgatggtc agcgtcggcc aggcggccag ccgctcggcg aagccccgct
     1561 ccaccggcgc gtccagctcc gccagcccca gctcggcctt gaccgcctcc acgtcgaccg
     1621 gcagccgggc cagggcctgc cgctcggcct cgcccagcgg ctcgacctcg tcggcgaacc
     1681 ccgccacggt gatgcggccc tgggcgtcgc gcatggtggc cagcagctgg accagcgtcc
     1741 acagcgggtt ggggacgacc ccgccccagt tgcccgagtg cagcgaccgg ttcgcccccc
     1801 gggcccgcag ctcgaagctg gcgatccctc tgaccccgaa caccagccgc cagccgccgc
     1861 ccgggtcgac cggcccgtcg ctccagacca ccaggtcggc ggcgagccgt ccccgctggc
     1921 cggcggcgaa ggcggccagg ttgggcgagc cgatctcctc ctcgccgtcc agcagcacct
     1981 tgaccgtgca gggcagctcg ccccgggtgg ccagcaggct ctccacggcc agcagctggg
     2041 ccaggtgctg gcccttgttg tcggcgccac cgcggccgta caggcggccg tcgcggacct
     2101 cgggctcgaa cggcgggctg gtccaggcgt ccagcgggtc gggcggctgc acgtcgtagt
     2161 ggccgtagac gagcacggtg ggggcgccgg gcggcccggc ccggtggccg aggaccagcg
     2221 gccagccggc cgtggagacg accgtcgcgt ccaggccggc cgcggccacc aggtcgcggg
     2281 cgtggccggc ggcctcgggc atgccctggc cggtggcgct cacgctcggg acccggaccc
     2341 agccggccag gcgctccacg aacgcggccc ggttggcgtc gacgtgggcg aacagggcct
     2401 ccagctccgg cggagccccg gccgtgccgc tcacggcgtg ttggggtcga gccagaggtt
     2461 ggtgatgtcg gggttggcca gccaggggct gaaggtccag ttcctgaccc
//
LOCUS       DASSWR010000418         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_440119,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000418 DASSWR010000000
VERSION     DASSWR010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440119"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1148)
                     /locus_tag="VFO47_06975"
     CDS             complement(276..1148)
                     /locus_tag="VFO47_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET8788473.1"
                     /translation="MRVATWNVNSVKQRVPRLLPWLDQRQPDVVCLQETKLTDRKFTE
                     LLGDELAGRGYEVALHGEGQWNGVAILSRLGLGEVVRGVPGAPGFPRPEARAVAATCG
                     GIRVWSVYVPNGRVPESDHYRYKLAWLAALRDDVAAGPDATIVCGDMNIAPTDADVFD
                     PAAYVGQTHVTPPERAALAGLQTLGLRDVVRDRWPDERVFTYWDYRAGMFHQDLGMRI
                     DLVLASATVAGRVRAAWVDRQARKGSGPSDHAPVIVDLDEAPDGDIGPVVPPPSAPVI
                     RQRPAKLPQARPPA"
     gene            1229..1768
                     /locus_tag="VFO47_06980"
     CDS             1229..1768
                     /locus_tag="VFO47_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8788474.1"
                     /translation="MTVLRGADRAGLASEINGSLARRHSTATVLFHHALAERLGLGPT
                     DHKCLDLLWERAPLTASELAAITGLTSGAVTGVVARLERSGWLRREPHPQDRRKQVLY
                     PTPEAAREIQRVFDALPDAGELLEGFDADQLAAVAEFLTRASDLAYRRGALLRAQVLA
                     AGGRQPGPAIGGQATTKER"
     gene            1768..>2214
                     /locus_tag="VFO47_06985"
     CDS             1768..>2214
                     /locus_tag="VFO47_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET8788475.1"
                     /translation="MNDEQQIRALFDQMCRAWSDGDAVAYGRCFTADCDYVSFDGYRE
                     RGRDAMVASHDKLFRGVLFGSALVGQVESIRHLDDGVALVHATGSVLVAWRRRLPKRR
                     LTRNTIVVVRGPEGWRCAAIHNGRVRPVGVPGPDSVPARVARSLVRA"
BASE COUNT          287 a          813 c          813 g          301 t
ORIGIN      
        1 aggtctcctt cacatccccg caccggcgct cggcattcgc gccggtccgg gtcaagttca
       61 tgcgtttgca tatacatcaa cgggtgcgac cgccacactt attccgccgt gcggcctccc
      121 ccggcgagtc ctcaccggca cggcggttcg gccggcaagg tcgccgacgt ctacggcgtc
      181 cccggccgag cgccatcagg tccaggccgc cgaagcggtc gccgactcga gtgggccgtc
      241 ccggtccagg ccggacaggc gggcgtgggc atccgctagg ccggcggccg cgcctggggg
      301 agcttggccg ggcgctgcct gatgaccggg gcggacggtg gcgggacgac cggcccgatg
      361 tcgccgtccg gcgcctcgtc caggtcgacg atcacggggg cgtggtcgct gggcccggag
      421 cccttgcggg cctgccggtc cacccaggcc gcccggaccc ggccggcgac ggtcgccgag
      481 gccagcacca ggtcgatccg catgcccagg tcctggtgga acatgccggc ccggtagtcc
      541 cagtaggtga acacccgctc atccggccag cggtcccgca cgacgtcgcg caggccgagc
      601 gtctgcagcc cggccagggc cgcccgctcc ggcggggtga cgtgagtctg gccgacgtag
      661 gcggccgggt cgaacacgtc ggcgtcggtc ggggcgatgt tcatgtcccc gcagacgatc
      721 gtcgcgtccg gaccggcggc cacgtcgtcc cgcagcgcgg ccagccaggc cagcttgtag
      781 cggtagtggt cggactccgg cacccgcccg ttcggcacgt acacggacca cacccggatg
      841 ccgccgcagg tcgcggccac ggcccgcgcc tccgggcgcg ggaacccggg tgcgcccggc
      901 accccgcgca cgacctcccc cagccccagc ctggagagga tggccacgcc gttccactgg
      961 ccctccccgt ggagcgcgac ctcgtagccg cggccggcga gctcgtcgcc gaggagctcg
     1021 gtgaacttgc gatcggtgag cttggtctcc tgcaggcaga cgacgtcggg ctgccgctgg
     1081 tcgagccagg gcagcagccg gggcacccgc tgcttgaccg agttcacgtt ccaggtcgcc
     1141 actcgcaccc ggccacagta gcgcagcttg accaggatcc gttcaccagt taacttagta
     1201 actaagctac atgaggagga gagtgggcat gacggtgctt cggggggcgg atcgggccgg
     1261 gttggcgtcc gagatcaacg ggtcgctggc ccgccggcac agcaccgcga ccgtgctgtt
     1321 ccaccacgcc ctggccgagc gcctggggct gggcccgacc gaccacaagt gcctggacct
     1381 gctctgggag cgggcccccc tgacggccag cgagctggcc gcgatcaccg gcctgaccag
     1441 cggggcggtc accggggtgg tggcccggct ggagcgctcg ggctggctgc gacgggagcc
     1501 ccatccccag gaccggcgca agcaggtgct gtacccgacc ccggaggcgg cgcgcgagat
     1561 ccagcgggtg ttcgacgccc tgcccgacgc cggggagctg ctggaggggt tcgacgccga
     1621 ccagctggcc gccgtcgccg agttcctgac ccgggcgagc gacctcgcct accggcgtgg
     1681 ggccctgctg cgggcccagg tgctggcggc cggcggtcgc cagcccggcc cggccatcgg
     1741 cggccaggcg accaccaagg agcgatgatg aacgacgagc agcagatcag ggccttgttc
     1801 gaccagatgt gccgggcctg gagcgacggc gacgcggtgg cctatggccg ctgcttcacc
     1861 gctgactgcg actatgtctc cttcgacggc taccgcgagc ggggtcggga cgcgatggtc
     1921 gcctcccacg acaagttgtt ccgcggcgtg ctgttcggct ccgcgctggt cggccaggtg
     1981 gagtcgatcc gccatctgga cgacggcgtg gcgctggtgc acgccaccgg ctcggtgctg
     2041 gtcgcctggc gcaggcggct gcccaagcga cgcctcaccc gcaacaccat cgtcgtcgtc
     2101 cgtggcccgg agggatggcg ctgcgcggcg atccacaacg ggcgggtccg cccggtgggc
     2161 gtccccgggc cggactcggt gcccgcccgg gtggcccgct cgctcgtccg ggcc
//
LOCUS       DASSWR010000419         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_467493,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000419 DASSWR010000000
VERSION     DASSWR010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467493"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /gene="sdhA"
                     /locus_tag="VFO47_06990"
     CDS             complement(<1..1463)
                     /gene="sdhA"
                     /locus_tag="VFO47_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET8788476.1"
                     /translation="MTQQHTFDVVIVGAGGAGLRAALESSRRARTAVITKLYPTRSHT
                     GAAQGGMCAALANVEEDNWEWHTFDTIKGGDYLVDQDAAEVMCREAIEAVYDLEHMGM
                     PFNRTPDGKIDQRRFGGHTHHHGAGAVRRSCFAADRTGHMILQTLFQQNVKQGTTFFD
                     EYYVLDLLLDPGDGRAAGVVAYELATGELHVFRAKAVVFCTGGFGKMFKITSNAHALT
                     GDGPGVCYRRGVPMEDMEFFQFHPTGLYRLGVLLSEAARGEGGILRNADGEAFMERYA
                     PTIKDLAPRDMVSRAIYTEIREGRGAGPEGDYVYLDVSHLDPKVIDAKLPDITEFART
                     YLGVEPKKEGVPIQPTAHYAMGGIPTTVDAQVVGDAANTVIPGLYAAGECACVSVHGA
                     NRLGTNSLLDIVVFGRRAGISAAVHAARVGHATLGDDPAGAVTRMLEELAAHPQGERA
                     AAIRGDLQAAMDDKAGVYRTETLLKEMEDELASLRER"
     gene            complement(1472..1897)
                     /locus_tag="VFO47_06995"
     CDS             complement(1472..1897)
                     /locus_tag="VFO47_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HET8788477.1"
                     /translation="MAADLTPAPLPRSTSADRRQPKPTRGNFELYAWVFMRISGVLLV
                     LLVLGHFGIMHVVDGGLDRINFAFVSGRWANPFWRVYDFAMLTLALAHGGNGLRVIID
                     DYARSGTRRLLYQSVNATVIGLTWALGTLTIFAFTPQPS"
     gene            complement(1900..2274)
                     /gene="sdhC"
                     /locus_tag="VFO47_07000"
     CDS             complement(1900..2274)
                     /gene="sdhC"
                     /locus_tag="VFO47_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET8788478.1"
                     /translation="MQRLGTLYRGREGMWTWVLHRVSGVAILFYLFAHVVDQALLNVS
                     PEAYDRVIDTYRNPFVGLLEIGLAVVVIFHALNGLRIILFDFWSQGVARQRQMLWVQT
                     VLFLILAIPPVIAIGAHVVEEL"
     gene            2407..>2939
                     /locus_tag="VFO47_07005"
     CDS             2407..>2939
                     /locus_tag="VFO47_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET8788479.1"
                     /translation="MSPDAAERLVAGRWTLHTALRRGPSGVIWRATEVSGGRPLAVEE
                     LRLPARPDPEEAGQAARWQRVADEARAAAALDHPGLVRLDDVVVEDGVVYVASELVGA
                     RTLNELIARHGPLPVRRVARLGLELLDALEAAHAAGLAHLDLRPACVLVTADGGARLA
                     GVGLGTLRGTPGADRGTT"
BASE COUNT          394 a         1025 c         1090 g          430 t
ORIGIN      
        1 tagcgctccc gcagggaggc gagctcgtcc tccatctcct tgaggagggt ctcagtccgg
       61 tagaccccgg ccttgtcgtc catggcggcc tggaggtcgc cgcggatggc ggcggcccgc
      121 tcgccctggg gatgggcggc cagttcctcc agcatccggg tgacggctcc ggccgggtcg
      181 tcgcccaggg tggcgtggcc cactctggcg gcgtggacgg cggcgctgat cccggcccgc
      241 cggccgaaca cgacaatgtc cagcagcgag ttggtgccca gccggttggc tccgtgcacc
      301 gacacgcacg cgcactcgcc ggccgcatac agtcccggga tgaccgtgtt ggcggcgtcg
      361 cccaccacct gagcgtcgac cgtggtcggg atgccgccca tggcgtagtg ggccgtcggc
      421 tggatgggca cgccctcctt cttgggctcg acccccaggt aggtgcgggc gaactcggtg
      481 atgtcgggca gcttggcgtc gatcaccttg gggtcgaggt ggctcacgtc caggtagacg
      541 tagtcgccct cggggccggc gccgcggccc tcgcggatct cggtgtagat ggcccggctg
      601 accatgtcgc ggggggcgag gtccttgatg gtgggggcgt agcgctccat gaacgcctcg
      661 ccgtcggcgt tgcgcaggat gccgccctcg ccccgggcgg cctcgctcag cagcaccccg
      721 agccggtaca ggccggtggg gtggaactgg aagaactcca tgtcctccat gggcacgccc
      781 cgccggtagc agaccccggg gccgtcgccg gtgagggcgt gggcgttgga ggtgatcttg
      841 aacatcttgc cgaagccgcc ggtgcagaag accaccgcct tggcccggaa cacgtgcagc
      901 tcgccggtgg ccagctcgta ggcgaccacc ccggcggccc gcccgtcgcc ggggtcgagc
      961 agcaggtcga gcacgtagta ctcgtcgaag aaggtggtgc cctgcttgac gttctgctgg
     1021 aacagggtct ggaggatcat gtggccggtg cggtcggcgg cgaagcagga gcggcgcacc
     1081 gccccggcgc cgtggtggtg ggtgtggccg ccgaagcgcc gctggtcgat cttgccgtcc
     1141 ggggtgcggt tgaacggcat gcccatgtgc tcgaggtcgt agacggcctc gatcgcctcc
     1201 cggcacatga cctcggccgc gtcctggtcg accaggtagt cgccgccctt gatggtgtcg
     1261 aaggtgtgcc actcccagtt gtcctcttcg acgttggcca gggcggcgca catgccgccc
     1321 tgggcggcgc cggtgtggga gcgggtcggg tacagcttgg tgatcaccgc cgtccgggcc
     1381 cgccgggagc tctccagcgc cgcccgcagg ccggcccccc cggccccgac gatcaccacg
     1441 tcgaaggtgt gctgctgggt catgggggct cctaggaggg ctggggggtg aaggcgaaga
     1501 tcgtgagggt gccgagcgcc caggtcaggc cgatcacggt ggcgttgacg ctctggtaga
     1561 gcaggcgccg ggtgccgctg cgggcgtagt cgtcgatgat cacccgcagg ccgttgccgc
     1621 cgtgggccag ggccagggtg agcatggcga agtcgtagac ccgccagaac gggttggccc
     1681 agcggccgga cacgaaggcg aagttgatcc ggtccaggcc gccgtcgacc acgtgcatga
     1741 tcccgaagtg gcccagcacg agcaggacca gcagcacccc ggagatgcgc atgaacaccc
     1801 aggcgtacag ctcgaagttg ccgcgggtgg gcttgggctg gcggcggtcg gccgaggtgg
     1861 agcgggggag cggggccggg gtgaggtcgg cggccatgct cagagctcct cgaccacgtg
     1921 ggccccgatg gcgatcaccg gcgggatggc caggatcagg aacagcaccg tctggaccca
     1981 gagcatctgg cgctggcggg ccaccccctg ggaccagaag tcgaacagga tgatccgcag
     2041 gccgttgagg gcgtggaaga tcaccaccac ggccaggccg atctccagca gcccaacgaa
     2101 cgggttgcgg taggtgtcga tcacccggtc ataggcctcg ggggagacgt tcagcagggc
     2161 ctggtcgacg acgtgggcga acaggtagaa gaggatggcc acgcccgaca cccggtgcag
     2221 gacccaggtc cacatcccct cgcggcctcg gtagagcgtc cccaggcgct gcatccctcg
     2281 tcacctttcc ggtccgggtc ccctccgact ctaccaccgg ccaatcgacc tgtcgtggca
     2341 ccaaacctcc tgctcagcgg cggttttggg cggggtaggg tttggtcgta tcgttggcca
     2401 tccacgatgt cacccgacgc cgccgagcgg ctcgtcgccg gccgctggac gctgcacacc
     2461 gcgctgcgcc gcggcccctc cggcgtgatc tggcgggcca ccgaggtgtc cggcggccgg
     2521 ccgctggcgg tcgaggagct ccgcctcccg gcccgcccgg acccggagga ggccggccag
     2581 gcggcccgct ggcagcgggt cgccgacgag gcccgggccg cggccgcgct cgaccatccc
     2641 ggcctggtcc ggctcgacga cgtggtcgtc gaggacggcg tcgtctacgt ggccagcgag
     2701 ctggtcggcg cccgcaccct caacgagctg atcgcccgcc acggccccct cccggtccgc
     2761 cgggtggccc gcctcggcct ggagctcctc gacgccctgg aggcggccca cgccgccggt
     2821 ctggcccacc tcgacctgcg ccccgcctgt gtcctggtca ccgccgacgg cggcgcccgc
     2881 ctggccggcg tcggcctcgg cactctccgc ggcaccccgg gagccgaccg gggcaccac
//
LOCUS       DASSWR010000420         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1005425, whole genome shotgun sequence.
ACCESSION   DASSWR010000420 DASSWR010000000
VERSION     DASSWR010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005425"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VFO47_07010"
     CDS             complement(<1..1974)
                     /locus_tag="VFO47_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8788480.1"
                     /translation="MAAAQTPAGLGRFSALTRGWFEGAFAAPTQAQVGAWDALAQDQD
                     VLVVAPTGSGKTLAAFLSAIDRLAGAPPVPERQRLRVLYISPLKALAADIERNLRAPL
                     AGLRAAAARLDQPLPDITVGMRTGDTPAEARRRFPAHPPDILITTPESLFLLLTSRAR
                     EALAGVDTVIVDEVHSVVATKRGAHLALSLERLDELLERPARRIGLSATVRPLDEVAR
                     FLGGSRPVTVVAPPSEKAFDLSVVVPVEDMAAIGEASDDPASGSASGLPDRSSIWPHI
                     DERLVELIRAHRSTIVFANSRRLAERLCANLNELAGTELARAHHGSVSREQRTEIEED
                     LKAGRLPAVVATSSLELGIDMGAVDLVIQVEAPSSVASGLQRIGRAGHQVGAVSRGIV
                     FPKFRGDLVESAVVVERMQAGAIEAMRYPRNPLDVLAQQVVAMVAMDDWTVDDLEALV
                     RRAAPFAELPRSALDSVLDMLSGRYPSDEFAELRPRLNWDRVEGRLAGRPGAQRLAVT
                     SGGTIPDRGLFGVFLVGERATRVGELDEEMVYESRVGEVFTLGASAWRIEDITHDRVL
                     VSPAPGQPGKLPFWHGDMLGRPVELGRALGGFLRELSGLAPEARTERLRAAGLDDFAA
                     GNLAAYLDEQREATGVLPDDRTIVVERFRDELGD"
     gene            2059..>2268
                     /locus_tag="VFO47_07015"
     CDS             2059..>2268
                     /locus_tag="VFO47_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788481.1"
                     /translation="MGWIERPEFGANVGLIDEIYRQYLDDPESVSPAWQDFFTENEPE
                     GYEDPGAAEATAEVEEQPPPKAEDKP"
BASE COUNT          317 a          790 c          894 g          267 t
ORIGIN      
        1 gtcgcccagc tcgtcgcgga agcgctcgac cacgatcgtg cggtcgtcag gcaggacccc
       61 ggtggcctca cgctgctcgt ccaggtaggc ggccaggttg ccggcggcga agtcgtccag
      121 gccggccgcc cgcagccgct cggtccgggc ctcgggggcc aggccggaca gctcgcgcag
      181 gaacccgccg agggcgcggc cgagctcgac cggccgcccc agcatgtcgc cgtgccagaa
      241 cggcagcttg cccggctggc cgggggccgg ggagaccagc acccggtcgt gggtgatgtc
      301 ctcgatccgc caggccgagg cgccgagggt gaacacctcc ccgacccggg actcgtacac
      361 catctcctcg tccagctcgc cgacccgggt ggcccgctcc ccgaccagga agacgccgaa
      421 caggccccgg tcggggatgg tgccgccgga ggtgacggcc aggcgctggg cgccgggacg
      481 gccggccagc cggccctcga cccggtccca gttgagccgg ggccgcagct cggcgaactc
      541 gtccgagggg taccgccccg acagcatgtc gaggacgctg tcgagggcgc tgcggggcag
      601 ctcggcgaag ggcgcggccc ggcggaccag ggcctcgagg tcgtcgacgg tccagtcgtc
      661 catggccacc atggccacga cctgctgggc gagcacgtcc agggggttgc gggggtagcg
      721 catggcctcg atcgccccgg cctgcatgcg ctcgaccacg accgcgctct cgaccaggtc
      781 gccgcggaac ttggggaaga cgatgccgcg gctgacggcg ccgacctggt ggccggcccg
      841 gccgatgcgc tgcaggccgg acgcgaccga gctgggcgcc tccacctgga tgaccaggtc
      901 gacggcgccc atgtcgatgc ccagctccag cgagctggtc gcgaccaccg cgggcagccg
      961 gccggccttg aggtcctcct cgatctcggt gcgctgctcg cggctgaccg agccgtggtg
     1021 ggcccgggcc agctcggtcc cggcgagctc gttgaggttg gcgcagagcc gctcggccag
     1081 gcggcgggag ttggcgaaca cgatcgtgga gcggtgggcc cggatcagct cgaccagccg
     1141 ctcgtcgatg tggggccaga tcgaggagcg gtcgggcagc ccggaggccg acccgctcgc
     1201 cgggtcgtcg gacgcctcgc cgatggccgc catgtcctcg accgggacca ccaccgacag
     1261 gtcgaacgcc ttctcgctgg gcggggcgac gacggtgacc gggcgggaac cgccgaggaa
     1321 gcgggccacc tcgtccaggg gccggacggt ggccgacagg ccgatgcggc gggccggccg
     1381 ctccagcagc tcgtccaggc gctccaggct gagggccagg tgggctccgc gcttggtcgc
     1441 gaccaccgag tggacttcgt ccacgatcac cgtgtcgacg ccggccaggg cctcacgggc
     1501 cctggaggtg agcagcagga acaacgactc gggggtggtg atgaggatgt cgggcgggtg
     1561 ggccgggaag cggcggcggg cctcggccgg ggtgtcgccg gtgcgcatcc ccacggtgat
     1621 gtcgggcagc ggctggtcga gccgggccgc ggccgcccgc aggccggcca ggggggcccg
     1681 gaggttgcgc tcgatgtcgg cggcaagggc cttgagcggg gagatgtaga ggacccggag
     1741 gcgctggcgc tcgggcaccg gcggggcgcc ggcgaggcgg tcgatggccg acaggaacgc
     1801 ggccagggtc ttgcccgacc cggtcggggc gaccaccagc acgtcctggt cctgggcaag
     1861 ggcgtcccag gcgcccacct gggcctgggt gggcgcggcg aaagcgccct cgaaccagcc
     1921 tcgggtcagg gccgagaacc gcccgagccc ggcgggcgtt tgcgctgcag ccacctgcac
     1981 atgctagtgc cggcccccga caacgtgccg gtggcaaact ggggtcgacg agcgtctcgg
     2041 taccgaggat ggtgcgcgat gggttggatc gaacggccgg agttcggtgc caacgtcggg
     2101 ttgatcgacg agatctaccg gcagtacctg gacgacccgg agtcggtgag cccggcctgg
     2161 caagacttct tcaccgagaa cgagcccgag ggctacgagg accccggggc ggccgaggcg
     2221 accgccgagg tcgaggagca gccgcctccc aaggccgagg acaagccg
//
LOCUS       DASSWR010000421         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1117877, whole genome shotgun sequence.
ACCESSION   DASSWR010000421 DASSWR010000000
VERSION     DASSWR010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117877"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1167..1931
                     /locus_tag="VFO47_07020"
     CDS             1167..1931
                     /locus_tag="VFO47_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HET8788482.1"
                     /translation="MPIDPTELANSIGALGSLDPERGLARTLQQVTDGAKQLFRADAA
                     GLMLVDAGGQLRWASASDQTAQTLEDRQERLAQGPCAVAFSQRLSAAIRNIHTEPDWD
                     KFAQVLVSEGICAALSVPVELDGGVIGTLDVYVGQPRDWDPSEVAALQAYAGLVASLL
                     SAAVTAEVKGRLADQLQAALEHRWLIEQAKGVLMGREDLDAQAAFERLRGAARSSTRR
                     LADVARDVTGGQPLPTNRRKLARGRAEQAKDRETTA"
BASE COUNT          362 a          851 c          776 g          364 t
ORIGIN      
        1 gccatcggcc ccgacgaccg tcctggtcgc ctccaccacc cgccccaacc cgaccagcag
       61 atccccatcc tggggcaggg tggcccgcag ctccgccacg gtccgcgcga ccagttgccc
      121 gtccgccgtc atccacgatc cttcgctcga gctgaccagc gtctgctggt ggccgtcgat
      181 ggtatatccc ggaagcggtc cggcaacccg acacgggcag gcaccagccc actcgccgcc
      241 gcgcggtgcg gcagcacatg gcggcccggc ggtccccgcc gcggccacag attcccgctc
      301 gtcaccaggg gtagtcgggt gtggccgttc cgggcccacc tgcctgtccg ggcggctgta
      361 gcgcctgaag ggggcgttct cggcccagac cccgccagcg cgtcccttca gctacccggc
      421 gcccacctcc ccgcccagcg gcggcaggac cgcaccaccc ggcaccgtca ccgtccgcgc
      481 tcagttaccg ccctccttca ccccccgagt gcgggagtct cgcccgcctt gcggttcagt
      541 ctcggcaagc tgcaaccttc cgatcggacc ggatggttgg ccgcgatccg gtgtcttgac
      601 gtgggcagtc tcacagccat tccctgccgt cacgccgctg gtcggaccac attcaaggga
      661 tgaggccgct gacctaacag cgacaacagg ccaagctggc acatgctgga cgagtgcgaa
      721 gcgactcgat tccgaagaca gcagttcaga acctcgccac cagatgattg gtcgtcgctt
      781 ctctgaagaa gctaggggtg gaggcagcct cgggcgaggg atgcaggcca gcggggtttg
      841 ggccaacctg cccgccgcga cgggcccgat cgcagcggtt ccctgcccga ggctgcaggg
      901 gccgtgtagc caccggcacc ggaccccaat ctggcgggct ggcggcagtc ggtttgagcc
      961 aggagacggc aggcgtacag taatccccga tgtccgtatt cgcgtcgtcg ccacccggac
     1021 ggtggcacct cagggaccta gccctcgccc cgatctaacc tgccagctgg cgacggcctg
     1081 ttggcccgct cggcatcctc ggcgcctgct cctggcctgc tgaccggttc gccagtcccc
     1141 ggctccccag aaaggcaggg agcgtcatgc cgatcgaccc caccgagctg gccaacagca
     1201 tcggcgctct gggcagcctg gacccagaac ggggcctggc ccgcaccttg cagcaggtga
     1261 ccgatggggc caagcagctg ttccgggccg acgcggccgg gctcatgctg gttgacgccg
     1321 gcgggcagct gcgctgggcc agcgcctccg atcagaccgc ccagaccctc gaggaccgcc
     1381 aggagcgcct ggcccagggc ccgtgtgcgg tggcgttcag ccagcggctg tcggccgcca
     1441 tccgcaacat ccacaccgaa cccgactggg acaagttcgc ccaggtcctg gtcagtgagg
     1501 ggatctgcgc ggccttgagt gtgccggtgg agctggacgg tggggtcatc ggcaccctgg
     1561 atgtctatgt cggccagccg cgggactggg atcctagcga ggtcgcggcc ttgcaggcct
     1621 atgccgggct ggtggccagc ctgctctcgg cggcggtgac cgccgaggtc aaggggcggc
     1681 tggccgacca gttgcaggcc gccttggagc accggtggct gattgagcag gccaaggggg
     1741 tgctgatggg ccgcgaagac cttgacgccc aggccgcatt tgagcggttg cggggggcgg
     1801 cccggtcctc cacgaggcgg ctggctgatg tggcccggga cgtgaccggc ggccaacccc
     1861 tgccaaccaa ccggcggaag ctggccaggg gacgggccga gcaggccaag gaccgggaga
     1921 ccaccgccta gcgggctgcg cgggtcgcgg ataccgccgg caggatcgct cgaggctcgt
     1981 gctgtgcgtt agcagccctg cagcctgctt cgtgtcgacc cgagaccaca tgccttggtt
     2041 gctttgttgc gctgatcttt gctgattcgg tccggtttgt ggccacgtta gggagctatc
     2101 cacccattgt cagctgaccc gtaaccgggc agcacagttc ctagcgaggc ccccagatcc
     2161 cgggcccccg tcgcgatcac cgaaccgcgg tatgcgacac cgcatggtcg tcctgcttcg
     2221 ctctccataa gaacccgtcc gtcgtggcgt gtgcttccgc acggcctcac ccaaccctga
     2281 agtccgccac aaccgtccat ccatcgcacc aacaggaagg gaagttcatg ccagcaccat
     2341 ccatcgagac cgc
//
LOCUS       DASSWR010000422         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1117925, whole genome shotgun sequence.
ACCESSION   DASSWR010000422 DASSWR010000000
VERSION     DASSWR010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117925"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFO47_07025"
     CDS             complement(<1..309)
                     /locus_tag="VFO47_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8788483.1"
                     /translation="MLSSYVRDAWHTATDEGTTVLDASTGEPVARVSSAGVDVAGMVD
                     HARRVGGPGLRELTFHQRAAALKRLALYLRDRRDGLYEQSARTGATTGDAMVDVDGGI
                     G"
     gene            complement(331..741)
                     /locus_tag="VFO47_07030"
     CDS             complement(331..741)
                     /locus_tag="VFO47_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788484.1"
                     /translation="MPESPVRELRLALTVEDYDQAIAFYRDVLGLPVTESWDGPDGSG
                     AVLDAGRATLELLSTAQAELVDRVEVGSRVAGPVRVALEVADSAGTARRLVAGGAEAL
                     AEPVVTPWRHRNVRVRAPDGMQLTLFTVLDDASA"
     gene            837..1820
                     /locus_tag="VFO47_07035"
     CDS             837..1820
                     /locus_tag="VFO47_07035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8788485.1"
                     /translation="MTTYGYHASHEQLPPSALLADVQLAEQAGFQAAMCSDHFAPWSH
                     AQGHSGFAWSWLGAALQATRLPFGCVNAPGQRYHPAIVAQAAATLAEMFPGRFWVALG
                     SGEALNEHITGTGWPDKQARNDRLLECVQVIRALLAGETVSHRGLVTVDRARLWSRPA
                     EPPPLLGAAVSAETAAWVAGWADGLITVNQPMDRLRRVVDAFRAGAAGKPMYLQVHLA
                     FAQDEATALANAHQQWGSNVLDGSLAWELALPEQFEAATRHVRPDDVRETVLVTADPS
                     RLTAELAAYAELGFDGVFVHEVGRDQRRTIELFGSQVLPALAAASDGRAGR"
     gene            1817..3460
                     /locus_tag="VFO47_07040"
     CDS             1817..3460
                     /locus_tag="VFO47_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET8788486.1"
                     /translation="MSDKATSDLWWKNAVIYCLDVELFADGNGDGVGDFPGLTDRIEY
                     LAGLGVTCLWLMPFYPTPNRDNGYDIADYYGVDPRLGSLGDVVELLRTARERGIRVIA
                     DLVVNHTSVDHPWFQAARADRRSPYRDYFVWADEPPADGPKDVVFPGQETSNWEFDER
                     AGQWYLHRFYKSMPDLNIANPKVRDEIHKVVGFWLELGLSGFRVDAAPFLIELTGAEQ
                     PEPGDPHEYLRDLRAFLARRRGDAILLAEANLPPPDQRRYFGDEDGDEMHMVFNFVAN
                     QRLFLSLVRQDAGPLADALASQPAIPETGQWANFVKNHDELSLDKLSDGEREEVFAAF
                     APEEDMRIYGRGIRRRVPPMLGGDRRRLELVYSLLFALPGAPVLLYGEELGMGDDLAV
                     EGRGSVRVAMQWSDGDGCGFTSARPRVPVVTGGEFGCGRVSVGGQRRDPGSLLNWTER
                     LVRTRKETPELGWGACEVLPAGDPAVLALRSDWRGSVVLTVHNLADRPAEARVELAGG
                     DSGAGLVELLADQAYEPLESPPGGVPVGAYGYRWFRLPG"
     gene            3485..3976
                     /locus_tag="VFO47_07045"
     CDS             3485..3976
                     /locus_tag="VFO47_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET8788487.1"
                     /translation="MATDDDDLRHLRRCVELATVALEAGDEPFGSVLVAADGTVLFED
                     HNHVASGDRTRHPEFEIARWAAANLDPAERAAATVYTSGEHCPMCAAAHGWVGLGRIV
                     YVSSSEQLGAWLAELGVPPPPVRTLPIREVVPGVPVEGPVPSLAEAVHDLHRRFHTGR
                     PAR"
     gene            complement(3973..>4279)
                     /locus_tag="VFO47_07050"
     CDS             complement(3973..>4279)
                     /locus_tag="VFO47_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788488.1"
                     /translation="PMPATCAAAGNWLSYEERMRFIACYFRLYLHVHQLFDRPFTEEH
                     ARRLEEMLDDGALPTATRVDRSGTGGRPTRWFPLPRGVDPEARALEAFRLEDNVEAR"
BASE COUNT          540 a         1618 c         1575 g          546 t
ORIGIN      
        1 gccgatgccg ccgtcgacgt cgaccatggc gtcgccggtg gtggccccgg tccgggccga
       61 ctgctcgtac aggccgtcgc ggcggtcccg caggtagagg gcgagccgct tcagggcggc
      121 ggcccgctgg tggaaggtga gctcgcgcag accgggtccg ccgacccggc gggcgtggtc
      181 gaccatgccg gccacgtcca ccccggcgct cgacacccgg gccaccggct cgccggtgga
      241 cgcgtcgagc acggtcgtgc cctcgtcggt cgcggtgtgc caggcgtcgc ggacatagga
      301 gctgagcatc gccgtcacgg tcggccctcc tcaggcgctg gcgtcgtcga gcacggtgaa
      361 cagggtgagc tgcatcccgt cgggggcgcg gacccggacg ttgcggtggc gccacggggt
      421 gacgaccggc tcggcgagcg cctcggcgcc gccggcgacg agccggcggg ccgtcccggc
      481 cgagtccgcg acctcgagcg cgacccgcac cgggccggcg acccgggacc cgacctcgac
      541 ccggtcgacc agctccgcct gggccgtcga cagcagctca agggtggccc ggcccgcgtc
      601 cagcaccgcc ccgctgccgt cgggcccgtc ccaggactcg gtcaccggca ggccgaggac
      661 gtcgcggtag aaggcgatcg cctggtcgta gtcctccacc gtcagcgcca accgcagctc
      721 gcgaaccggg ctctcgggca tctcgctcct ctcgtcgccg gagggtggcc gggcccagca
      781 tgcaccaggc ggcgaccgga ggcttgcccg ggccgtgcgg cgggtagccg gaggccatga
      841 cgacctacgg ctaccacgcc tcccacgagc agctgccccc gagcgccctg ctggccgacg
      901 tccagctggc cgagcaggcc gggttccagg cggcgatgtg ctccgaccac ttcgcgccct
      961 ggagccacgc ccagggccac agcgggttcg cctggtcctg gctgggcgcg gccctgcagg
     1021 cgacccgact gccgttcggc tgcgtcaacg cgcccggcca gcgctaccac ccggcgatcg
     1081 tcgcccaggc ggcggccacc ctggccgaga tgttcccggg gcgcttctgg gtcgcgctgg
     1141 gcagcggcga ggccctcaac gagcacatca ccgggaccgg gtggccggac aagcaggccc
     1201 gcaacgaccg gctgctggag tgcgtgcagg tcatccgggc gctgctcgcc ggcgagaccg
     1261 tcagccaccg cggcctggtc accgtcgacc gggcccggct gtggtcgcgc ccggcggagc
     1321 cgccgccgct gctcggcgcc gccgtgagcg ccgagaccgc cgcctgggtg gccggctggg
     1381 ccgacggcct gatcaccgtc aaccagccca tggaccggct gcgccgggtc gtggacgcct
     1441 tccgggccgg cgccgccggc aagccgatgt acctccaggt ccacctggcc tttgcccagg
     1501 acgaggcgac cgccctggcc aacgcccacc agcagtgggg cagcaacgtg ctcgacgggt
     1561 cgctggcctg ggagctggcc ctgcccgagc agttcgaggc cgcgacccgg cacgtgcggc
     1621 ccgacgacgt ccgcgagacg gtgctggtca ccgccgaccc gtcgcgcctg accgcggagc
     1681 tggccgccta cgccgagctc ggcttcgacg gcgtgttcgt ccacgaggtc ggccgcgacc
     1741 agcgccgcac catcgagctg ttcggctccc aggtgctccc cgccctggcc gcggcgtccg
     1801 acgggagggc ggggcggtga gcgacaaggc gaccagcgac ctgtggtgga agaacgcggt
     1861 catctactgc ctggacgtcg agctgttcgc cgacggcaac ggcgacgggg tcggtgactt
     1921 tcccgggctg accgaccgga tcgagtacct ggccgggctc ggggtgacct gcctgtggct
     1981 gatgccgttc tacccgaccc cgaaccgcga caacggctac gacatcgccg actactacgg
     2041 cgtcgacccg cggctgggca gcctcggcga cgtcgtggag ctgctgcgga cggcccggga
     2101 gcgcggcatc cgggtgatcg ccgacctggt cgtgaaccac acctcggtcg accacccctg
     2161 gttccaggcc gcccgggccg accggcgctc gccctaccgc gactacttcg tctgggccga
     2221 cgagccgccc gccgacgggc ccaaagacgt cgtcttcccg ggccaggaga cctccaactg
     2281 ggagttcgac gagcgcgccg gccagtggta cctgcaccgc ttctacaaga gcatgccgga
     2341 cctgaacatc gccaacccga aggtccgcga cgagatccac aaggtggtcg ggttctggct
     2401 cgagctcggc ctgtccgggt tccgggtcga cgccgccccc ttcctgatcg agctgaccgg
     2461 cgccgagcag cccgagccgg gcgatccgca cgagtacctg cgcgacctcc gcgccttcct
     2521 ggcccgccgc cggggcgacg ccatcctgct cgccgaggcc aacctgcccc cgcccgacca
     2581 gcgccgctac ttcggcgacg aggacggcga cgagatgcac atggtgttca acttcgtcgc
     2641 caaccagcgg ctgttcctgt cgctcgtccg ccaggacgcc gggccgctgg ccgacgccct
     2701 ggcctcgcag ccggccatcc ccgagaccgg ccagtgggcc aacttcgtga agaaccacga
     2761 cgagctcagc ctggacaagc tgagcgacgg cgaacgcgag gaggtgttcg ccgcgttcgc
     2821 gcccgaggag gacatgcgca tctacggccg cggcatccgc cgccgggtcc cgcccatgct
     2881 gggtggcgac cgccgccggc tggagctcgt ctacagcctg ctgttcgccc tgccgggcgc
     2941 ccccgtgctg ctgtacggcg aggagctcgg gatgggcgac gacctggccg tggagggccg
     3001 cggcagcgtc cgcgtggcca tgcagtggag cgacggggac ggctgcggct tcacctccgc
     3061 ccggcccagg gtgccggtcg tgaccggcgg cgagttcggc tgcggtcggg tcagcgtcgg
     3121 cggccagcgg cgcgacccgg ggtcgctgct caactggacg gagcggctgg tccggacccg
     3181 caaggagacc ccggagctgg gctggggcgc gtgcgaggtg ctgcccgccg gcgacccggc
     3241 cgtgctcgcc ctgcgctccg actggcgggg ctcggtcgtg ctgaccgtcc acaacctggc
     3301 cgaccggccc gccgaggccc gggtcgagct ggccggcggg gactccggcg ccggcctggt
     3361 ggagctgctc gccgaccagg cctacgagcc gctcgagtca ccccccggcg gcgtcccggt
     3421 cggtgcctac gggtaccgtt ggttccggct gcctggctga gcgcggcggg cacctcggga
     3481 ggggatggcc acggacgacg acgacctgcg gcacctgcgc cgctgcgtgg aactggccac
     3541 cgtggccctg gaggccggcg acgagccgtt cgggtcggtg ctggtggccg ccgacgggac
     3601 cgtgctcttc gaggaccaca accacgtggc ctccggcgac cggacccgcc accccgagtt
     3661 cgagatcgcc cgctgggcgg ccgccaacct ggacccggcg gagcgggcgg cggcgaccgt
     3721 gtacacctcg ggggagcact gcccgatgtg cgccgccgcc cacggctggg tcgggctggg
     3781 ccgcatcgtc tacgtcagct cgtccgagca gctgggcgcc tggctcgccg agctgggggt
     3841 gccgccgcca ccggtccgga ccctcccgat ccgggaggtc gtccccggcg tgcccgtcga
     3901 gggcccggtc cccagcctcg ccgaggcggt ccacgacctg caccgccggt tccacaccgg
     3961 acgtccggcc cgctagcggg cctcgacgtt gtcctcgagc cggaacgcct caagggcccg
     4021 ggcctcgggg tcgacccccc gtgggagcgg gaaccaccgg gtcgggcgtc caccggtgcc
     4081 gctgcggtcg acgcgggtgg ccgtcggcag ggcgccgtcg tccagcatct cctccagccg
     4141 gcgggcgtgc tcctcggtga agggccggtc gaacagctgg tggacgtgca ggtaaaggcg
     4201 gaagtagcag gcgatgaagc gcatccgctc ctcgtacgac agccagttcc cggcggcggc
     4261 gcaggtcgcc ggcatgggc
//
LOCUS       DASSWR010000423         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_892474,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000423 DASSWR010000000
VERSION     DASSWR010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892474"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..946)
                     /locus_tag="VFO47_07055"
     CDS             complement(11..946)
                     /locus_tag="VFO47_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET8788489.1"
                     /translation="MSTPLLLVGALAVFLAVFAIAYSVQSIVAERRQVYRTLQAIRAV
                     ELRPTDLRNRELARPARERLIKPFYNAALGLARRFTPAGARDAIRRKLIMAGSPFGWY
                     PDRVLVAKVACLIGGMLLGLLFLVIFGFAWPIRVLGFLAISLLGYRLPNLVLTNAVQR
                     RQNEIRTALPDSIDLLTICVEAGLGFDAALAHVSKNTSGPLADEFYRTLQEVQLGRSR
                     NEAMHNLADRTNVPELRAFVLAMVQADIFGVSVANVLRVQAKDMRVKRRQLAEERAMK
                     VPIKVLFPVLFCIFPALFVVILGPAIMRIVDVFAK"
     gene            complement(953..>2094)
                     /locus_tag="VFO47_07060"
     CDS             complement(953..>2094)
                     /locus_tag="VFO47_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET8788490.1"
                     /translation="GSTVSFRLEVRANNETGAYENPRFALSGQASPATVPVPAELPPV
                     PALSRLESSQGRYLIAVVGFVAILLACLLLFGPRSRGGKPYRALRQRLSPYSLTPAIA
                     DDQPRLTAFGSSEWAGRVTAMAETLVRRGNLEEAFLDRLEAAGLNMRVAEFVLISLGS
                     AFIPPLLVLVLTRNFLLTVVVVLLGVVGPFLYLSVMASRRRAKFDEQLPATLQLLAGA
                     LQAGHSLQQAVDTVVHEAGDPIAGEFQRVLTEARLGRPLEEAFEAMAKRTGSVDFGWT
                     VMAIRLQRQVGGNLAEVLSTVSQTIRDRYSLKRQIKALSAEGRLSSLILSILPILLFG
                     ALLLLNPQFLRPLYTTGLGIMMMAGSAVLMILGVLWLRKITEIKV"
BASE COUNT          369 a          689 c          756 g          280 t
ORIGIN      
        1 atcagggatc ctactttgcg aagacgtcga cgatgcgcat gatggcgggc cccaggatca
       61 cgacgaacaa ggcggggaag atacagaaga gtacggggaa caggaccttg atgggaacct
      121 tcatcgcccg ttcctcggcc agctggcgac gcttgacccg catgtccttc gcctggacgc
      181 gcagcacgtt ggccaccgac accccgaaga tgtccgcctg gaccatggcg agcacaaaag
      241 cccgcagctc agggacgttg gtgcggtcgg caaggttgtg catggcctcg ttgcggctcc
      301 ggccgagctg gacctcctgc agggtccggt agaactcgtc ggccaggggg ccgctggtgt
      361 tcttggagac gtgggccagg gccgcatcga agccgaggcc cgcctccacg cagatcgtga
      421 gcaggtcgat cgagtcggga agggccgtcc ggatctcgtt ctggcggcgc tggacggcgt
      481 tggtgagcac gaggttcggc agccggtacc cgagcaggct gatggccagg aagcccagga
      541 cccggatggg ccaggcgaac ccgaagatga ccaggaacag gaggccgaga agcatcccgc
      601 cgatgaggca ggccaccttg gcgaccagga cccggtcggg gtaccagccg aacgggctgc
      661 cggccatgat cagcttgcgc ctgatggcgt ccctcgcccc cgccggggtg aagcggcgcg
      721 ccaggccgag agccgcgttg tagaagggct tgatcaggcg ctcgcgggcg gggcgggcca
      781 gctcgcggtt gcgcaggtcg gtcgggcgca gctcgaccgc gcggatggcc tggagcgtcc
      841 ggtagacctg gcggcgctcg gcaacgatcg actggaccga gtaggcgatc gcgaacacgg
      901 ccaggaacac ggcgagggcg ccgacgagaa ggagcggagt cgacatggcg gttcagacct
      961 tgatctcggt gatcttccgc agccatagca ccccgaggat catgagcacg gcggatccgg
     1021 ccatcatcat gatcccgagc ccggtggtgt agagcggccg caggaactgc gggttgagga
     1081 ggagcagagc gccgaacagc aggatgggca ggatggacag gatcaggctg gagagccggc
     1141 cttcggcgga cagggccttg atctgccgct tgagcgagta gcggtcacga atcgtctggg
     1201 acacggtgct gagcacctcg gccaggttgc cgccgacctg tcgctgcagc ctgatcgcca
     1261 tgaccgtcca gccgaagtcg acgctgccgg tgcgcttggc catggcctcg aacgcctcct
     1321 ccagcggcct acccagccgc gcctcggtca gcacccgctg gaactcgccg gcgatcgggt
     1381 cgccggcctc gtggacgacc gtgtccaccg cctgctggag gctgtggccg gcctggaggg
     1441 cgccggcgag caactgcagg gtggccggga gctgctcgtc gaacttggcc cgccgccgcg
     1501 acgccatgac cgacagatat aggaacggcc cgaccacgcc cagcagcacc accaccacgg
     1561 tgaggaggaa gttgcgggtc aggaccagga ccagcagcgg cgggatgaac gccgacccga
     1621 gcgagatgag cacgaactcg gccacccgca tgttcagccc cgcggcctcc aggcggtcga
     1681 gaaacgcctc ctcgaggttg ccccggcgga ccagggtctc ggccatggcg gtcaccctgc
     1741 cggcccactc gctggagccg aacgccgtca gccgcggctg gtcgtcggcg atcgccgggg
     1801 tgagcgagta cggggacagc cgctgccgca gcgcccggta cggcttgccg ccgcgcgacc
     1861 ggggcccgaa cagcagcagg caggccagca agatcgcgac gaacccgacg acggcgatga
     1921 gatagcgccc ttgcgagctc tccagtcgcg acagcgccgg caccggcggg agctcggccg
     1981 gcaccggaac ggtggccggg ctggcctgcc cggagagcgc gaacctcggg ttttcgtacg
     2041 ctcccgtctc gttgttggcc cgaacctcca gcctgaagct caccgtgctc ccca
//
LOCUS       DASSWR010000424         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_864120,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000424 DASSWR010000000
VERSION     DASSWR010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864120"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFO47_07065"
     CDS             complement(<1..478)
                     /locus_tag="VFO47_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8788491.1"
                     /translation="MRTARRDEDLAHLLSQAERRVTERLSAALEAEGLTLAQWWVLTL
                     LSDGAGHPMAEAAEFAMLPAPTLTKVVDRMVSLNLVYRRPDLRDRRRVLIYSAARGRR
                     LHQRLDRDLSPAVRVLGDTADDQDAAQLARLLVRILERHQSPRVGGSPCTLTAHMIR"
     gene            547..1614
                     /locus_tag="VFO47_07070"
     CDS             547..1614
                     /locus_tag="VFO47_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8788492.1"
                     /translation="MGNDQLRVALVIPLQGPAGIFGPSCEACAELAAEEVNREGGVLG
                     RQLRFVVVDGGARPRRVADEVDALVTAGSVAAVTGWHISAVRQAIAPRVTGRVPYVYT
                     PLYEGGERTPGVFLVGETPSQQLLPSMRWMAEELGIRRWCIVGDDYVWPRGSAAAARR
                     YARVSGAEICDEVFVRLGTEQYGDVLRRVERSGAEAVLMLLVGDDAVQFNRAFARAGL
                     DQRLLRLSTLMEENMLLASGADSTRGLFASAGFFDSLATSDSLDFIGRFSARYGPEAP
                     VLNSLGESCYEGVRLLVQLIRRARSLDVDRICAVADTVGYDGPRGAVQLRGRHLEQRV
                     YLARADSMDFEVLDQLTPTRA"
     gene            1704..1985
                     /locus_tag="VFO47_07075"
     CDS             1704..1985
                     /locus_tag="VFO47_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET8788493.1"
                     /translation="MTEYEYRCPACGPFTVRRSMGSATPSAACPACRREGRRQFSVLP
                     VRRVQAALAGALERQEASADHPEVVRKVPPRPVRRRAPADPRQAALPRR"
BASE COUNT          259 a          726 c          748 g          292 t
ORIGIN      
        1 gcctgatcat gtgggcggtc agggtgcaag gggaaccgcc gacgcggggc gactggtggc
       61 gttccaggat gcgcaccagc aggcgggcga gctgggcggc gtcctggtcg tcggcggtgt
      121 ctcccaggac ccggaccgcc gggctgaggt cgcggtcgag gcgctggtgg aggcgccggc
      181 cgcgggcggc cgagtagatc agcacccggc ggcggtcgcg caggtcgggg cggcggtaga
      241 cgaggttcag cgacaccatc cggtcgacca ccttggtcag ggtgggggcc ggcagcatgg
      301 cgaactcggc ggcctcggcc atcgggtggc cggccccgtc ggacagcaac gtcaggaccc
      361 accactgggc cagggtcagg ccctcggcct ccagggcggc cgacaaccgt tccgtcacgc
      421 ggcgttcggc ctggctcagc aggtgggcca ggtcctcgtc acgtcgggcg gttctcattg
      481 cggcaggctg gttccagggc tagggtacct cgtagcagaa actattctag gtcgagagcg
      541 aacgcgatgg gtaacgacca gctccgggtc gcgttggtca tccccctcca gggcccggcc
      601 ggaatcttcg gaccctcctg cgaagcctgt gccgagctcg ccgccgagga ggtcaaccgc
      661 gagggcggcg tgctcggacg ccagctccgg ttcgtggtgg tcgacggggg cgctcgcccc
      721 cgccgggtcg ccgacgaggt cgacgccctg gtgacggccg ggtcggttgc ggccgtcacc
      781 ggctggcaca tctcggcggt gcgccaggcg atcgccccga gggtgaccgg ccgggttccg
      841 tacgtctaca cgccgctgta cgagggcggc gagcggacgc cgggggtctt tctggtcggc
      901 gagaccccgt cgcagcagct gctcccctcg atgcggtgga tggccgagga gctcggcatc
      961 cgccgctggt gcatcgtcgg cgacgactac gtctggccgc gcggatcggc ggccgccgcc
     1021 cgccggtacg cccgcgtcag cggcgccgag atctgcgacg aggtgttcgt ccgtctcggc
     1081 accgagcagt acggcgacgt gctccgccgg gtcgagcgca gcggcgccga ggcggtgctg
     1141 atgctgctgg tgggcgacga cgccgtgcag ttcaaccggg ccttcgcccg cgccggtctc
     1201 gaccagcggc tgctgcggct cagcaccctg atggaggaga acatgctgct ggcctccggt
     1261 gccgacagca ccaggggcct gttcgcctcg gcgggcttct tcgactccct ggccacctcc
     1321 gacagcctgg acttcatcgg ccgcttcagc gcccgctacg ggcccgaggc cccggtgctc
     1381 aacagtctcg gcgagtcctg ctacgagggc gtgcgcctgc tggtccagct catccggcgg
     1441 gcccggagcc tggacgtcga ccgcatctgc gcggtcgccg acaccgtcgg ctacgacggc
     1501 ccgcggggtg ccgtgcagct gcgcggccgc catctcgagc agcgggtcta cctggccagg
     1561 gccgacagca tggacttcga ggtcctcgac cagctcaccc cgacgagggc ttgacaggcc
     1621 cgtcgggaag cggtcaatac tttctcaaga aattattctc agcacgtgct atctctcgga
     1681 cgggcctccc cggaggtgac ctggtgaccg agtacgagta tcgctgcccg gcctgcgggc
     1741 cgttcaccgt ccgccgctcg atggggagcg ccaccccctc cgccgcctgc ccggcgtgcc
     1801 gccgggaggg ccggcgccag ttctcggtcc tgccggtccg ccgcgtccag gcggccctgg
     1861 ccggcgccct ggaacgccag gaggccagcg ccgaccaccc cgaggtggtg cgcaaggtcc
     1921 cgccgcggcc ggtccggcgg cgcgcccccg ccgacccccg ccaagccgcc ctgccccgcc
     1981 gctagcgacc ccgcacgagg agggatcgac atgccggaag tggtc
//
LOCUS       DASSWR010000425         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1372624, whole genome shotgun sequence.
ACCESSION   DASSWR010000425 DASSWR010000000
VERSION     DASSWR010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372624"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFO47_07080"
     CDS             complement(<1..850)
                     /locus_tag="VFO47_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HET8788494.1"
                     /translation="MRQQLPTAGTARPIEDYGLLGDTRTAALVGSDGAIDWLCVPRFD
                     GQPVFGRLVGGPAAGTFRFGPAGAATVIARRYRPHTATLETTWDTPGGRLTLTEGMIA
                     DVTGRLLPSSLLVRRLTAAGGPVDAVIEFDPRLGEHHRPPRIEHRGDVVVCSWSTTAL
                     ALGASPPIHLETPGPVRLRVAPGHPVTLALSVADREPLIYVDPEVAWAALAADEHGWQ
                     AWCADIDGDLPHRDTVARSLLTLRLLTYSPSGAPVAAPTTSLPEDLGGIRNWDYRYAW
                     PRDASIG"
     gene            complement(852..1328)
                     /locus_tag="VFO47_07085"
     CDS             complement(852..1328)
                     /locus_tag="VFO47_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788495.1"
                     /translation="MDWAGWALFGLVATAALTAVMIVAQLAGLTRLDLPLLLGAIVAE
                     DPDRARVAGFFLHLVIGQGFALGYAACFSLLDRATWWLGGLLGLLHVAVSLTVFVPLL
                     EGVHPRMASNRAGRSSTAVLEPPGLLGLNYGGQTPVVTIAAHLTYGVALGVLLGAR"
     gene            complement(1330..2436)
                     /gene="tal"
                     /locus_tag="VFO47_07090"
     CDS             complement(1330..2436)
                     /gene="tal"
                     /locus_tag="VFO47_07090"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET8788496.1"
                     /translation="MTKLQRLYNEQGQSPWLDNLTRVYLRDGTLGRLVADGIRGVTAN
                     PTIFAKAIEGSDAYDEQFAALIAQGRPVLDAYWDLVVDDIGDALGALRPTFDASAGTD
                     GFVSVEVAPELARDTDATIAAARQLHQRIAQPNLFVKIPATGEGVPAIQAMIAEGRSI
                     NVTLIFSLARYAEVVDAYLAGLETRAEGGGDLASVHSVASFFVSRVDTEVDRRLDAIG
                     TGDALALKGQAAVAQAKLAYRLFRDRFAGARWERLAARGAQVQRPLWASTSTKNPAYP
                     DTLYVDSLIGPDTVNTLPEATIAAFEDHGTLARTIDSGADHAGTVMDRLAEVGVDMDD
                     VGRTLEDQGVASFHQSFAHVLQALESKARQLAPR"
     gene            complement(2441..2599)
                     /locus_tag="VFO47_07095"
     CDS             complement(2441..2599)
                     /locus_tag="VFO47_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788497.1"
                     /translation="MRKMSKLHQLHDRYGQRPWLANLTRGDLTGDHAVWHRSETDMFP
                     GRHDRREK"
BASE COUNT          373 a          899 c         1046 g          384 t
ORIGIN      
        1 tgccgatgct ggcgtcgcgg ggccaggcgt agcggtagtc ccagttgcgg attccgccga
       61 ggtcttccgg gagcgaggtt gtcggtgccg cgaccggcgc gcccgacggg gagtaggtca
      121 gcaggcgcag ggtgagcaag ctgcgggcca ccgtgtcgcg gtgcgggagg tcgccgtcga
      181 tgtcggcaca ccaggcctgc cagccgtgct cgtcggccgc cagtgccgcc caggccacct
      241 cggggtcgac gtagatcagc ggttcgcggt cggcgacgct gagcgcgagg gtgacgggat
      301 gaccgggcgc gacccgcaac ctcaccggcc ctggggtttc gaggtggatc ggcggcgatg
      361 cgcccagcgc gagcgcggtg gtcgaccagc tgcacacgac cacgtcgccg cggtgctcga
      421 tgcgcggcgg ccggtggtgc tcgccgaggc gggggtcgaa ctcgatcacg gcgtcaaccg
      481 ggccgccggc ggcggtgagc cggcgcacca gcaagctgga cggtaacagc cggccggtga
      541 cgtcggcgat catcccctcg gtcagggtga gccgcccgcc cggcgtgtcc caggttgttt
      601 cgagggtggc ggtatggggt cggtagcggc gggcgatgac cgtggccgct ccggctggcc
      661 cgaagcggaa ggtgcccgcc gccggcccgc cgaccaggcg gccgaacacc ggttggccgt
      721 cgaagcgggg aacgcacagc cagtcgatgg cgccgtcgga gccgaccagt gcagcggtcc
      781 gggtgtcgcc gagcagcccg tagtcctcga tgggccgggc ggtgccggcg gtcgggagct
      841 gttggcgcat gtcagcgagc cccgagaagc acgccgaggg cgacgccgta ggtgaggtgg
      901 gcggcgatgg tgacgacggg ggtctggccg ccgtagttga ggccgagcag cccaggcggc
      961 tccagcaccg cggtcgatga tcgaccggcc cggttcgacg ccatgcgcgg gtgcacgccc
     1021 tcgaggaggg ggacgaacac ggtgagggag accgcgacat ggagcagccc gagcagcccg
     1081 cccagccacc aggtggcccg gtccagcagc gagaagcagg cggcatagcc cagggcgaac
     1141 ccctgtccga tcaccaggtg gaggaagaac ccggcgaccc gggcccggtc ggggtcttcg
     1201 gcgacgatgg cgccgagcag cagcggcagg tcgagccggg tcagcccggc cagctgggcc
     1261 acgatcatca ccgcggtcag ggcggcggtg gcgacgaggc cgaacagcgc ccagccagcc
     1321 cagtccatgt cagcgcggcg ccagctggcg cgccttgctc tcgagggcct ggaggacgtg
     1381 ggcgaacgac tggtggaaac tggccacgcc ctggtcctcg agggtgcggc cgacgtcgtc
     1441 catgtccaca ccgacctcgg ccagcctgtc catcaccgtg ccggcgtggt cggcaccgct
     1501 gtcgatggta cgggcgaggg tgccgtggtc ctcgaatgcg gcgatggtcg cctcgggcag
     1561 ggtgttgacc gtgtcgggcc cgatgagact gtcgacatac agggtgtcgg ggtaggcggg
     1621 gttcttggtc gacgtcgacg cccacagcgg tcgctgcacc tgcgcccccc gggccgccag
     1681 ccgctcccac cgtgcgccgg cgaagcggtc acggaacagc cggtaggcga gcttggcctg
     1741 ggcgaccgcg gcctggccct tcaatgccag cgcgtcgccg gtgccgatgg cgtcgaggcg
     1801 acggtcgacc tcggtgtcga cccggctgac gaagaacgac gccacgctgt gcaccgacgc
     1861 caggtcaccg cctccctcgg cccgggtctc gagcccagcc aggtaggcgt cgaccacctc
     1921 ggcgtagcgg gccagggaga agatcagggt gacgttgatg ctgcgtccct cggcgatcat
     1981 cgcctggatg gcgggcacgc cctcgccggt ggccgggatc ttcacgaaca ggttcggctg
     2041 ggcgatccgc tggtgcagct ggcgggcggc ggcgatggtg gcgtcggtgt cgcgggccag
     2101 ttcgggtgcc acctcgaccg acacgaaccc gtcggtgccg gcgctggcat cgaaggtcgg
     2161 ccgcagcgcg ccgagtgcgt cgccgatgtc gtccacgacc aggtcccagt aggcgtccag
     2221 caccggacgc ccctgggcga tcagcgccgc gaactgctcg tcgtaggcgt cggagccttc
     2281 gatggccttg gcgaagatcg tcgggttggc cgtcaccccc cggatcccgt cagccaccag
     2341 ccggccgagc gtgccgtcgc ggagatacac ccgggtcagg ttgtcgagcc agggactctg
     2401 gccctgctcg ttgtagaggc gctggagctt ggtcatgtca tcacttctct cgtcggtcgt
     2461 gccggccggg gaacatatcg gtttcggacc ggtgccacac ggcgtggtcg ccggtgaggt
     2521 cgccgcgggt gaggttggcc agccaggggc gctggccata gcggtcgtgg agctggtgca
     2581 gcttgctcat ctttctcatt gggtgctcct tgccgccagc gccccggacc ggtgcccggc
     2641 cggttcgctg accacttggt ccagggggac gtcggggtca ctcagccgct ccgggtcgac
     2701 cg
//
LOCUS       DASSWR010000426         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_73600,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000426 DASSWR010000000
VERSION     DASSWR010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73600"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VFO47_07100"
     CDS             complement(<1..885)
                     /locus_tag="VFO47_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET8788498.1"
                     /translation="MSTVLFIGGLGRSGSTLLERMLGRLDDVCSVGELVHLWERGLKE
                     NNRCGCGERFRECAFWGRVGEVAFGGWDRLDVGEVLALKASVDRNRFVPLMVAPALSP
                     AYRARMRRYLELLGRLYDAVREVSGRPLVVDASKHASHAFLVRRMRGVDLRLVHLVRD
                     SRGVAFSWSKRMRRAEVVEGDALMATDTPLRMSARYLGYNLLFHLLGALGVRSILLRY
                     ESLVRDPSGELTRVLALSGRPVAAGELGFVGDGWVELAPSHALTGNPMRFRSGRVPLR
                     VDEDWRGQLRRRHRLVTSV"
     gene            complement(882..1433)
                     /locus_tag="VFO47_07105"
     CDS             complement(882..1433)
                     /locus_tag="VFO47_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8788499.1"
                     /translation="MTNREQPLLLVTVGTDHHPFDRLVRWADGWLAAHPGSLRCLMQT
                     GTSAPPTGQASWQAYLEFDALQAAMAGAAAVVCHGGPGTILGARHMGAVPIVVPRQHR
                     LGEHVDDHQVAFSRRLAAEGGGIHLAETEADLHRLLDRVAGEPAAFRAGPGGRATATA
                     VREFGRLVDGLVGDRPGPRAGAR"
     gene            complement(1430..1879)
                     /gene="pssD"
                     /locus_tag="VFO47_07110"
     CDS             complement(1430..1879)
                     /gene="pssD"
                     /locus_tag="VFO47_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HET8788500.1"
                     /translation="MRALLVCSSGGHLIQLHNLKPWWERHDRLWVTFEKLDSTSLLAG
                     EQVAWAYHPTTRNLANLLRNTRLAWKLLRSYRPDVVVSSGAGVAFPFFLLAKLLGKRT
                     VYVEVYDRIDSPTMTGRLCYPFSDLFLLQWEEQRRNYPKGKVIGSLL"
     gene            complement(1887..>2051)
                     /locus_tag="VFO47_07115"
     CDS             complement(1887..>2051)
                     /locus_tag="VFO47_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018738077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET8788501.1"
                     /translation="PGITGLWQVSGRSELSFEDYVRLDLFYVENWSIAYDLFILSKTV
                     PLLVSTRGAY"
BASE COUNT          305 a          719 c          781 g          246 t
ORIGIN      
        1 gaccgaggtg accaggcggt ggcggcggcg cagctggccc cgccagtcct cgtcgacccg
       61 gagcgggacc cgaccgctgc ggaagcgcat cgggttgccg gtcagggcgt ggctgggggc
      121 gagctcgacc cagccgtcgc cgacgaagcc gagctcgccg gcggccaccg gacgccccga
      181 gagcgccagc acccgggtga gctcgcccga cgggtcgcgg accagcgact cgtaccgcag
      241 caggatgctc cgcaccccga gggcgcccag caggtggaag agcaggttgt agccgaggta
      301 gcgggcgctc atgcgcagcg gggtgtcggt ggccatgagg gcgtcgccct cgaccacctc
      361 ggcccgccgc atccgcttgg accaggagaa cgccaccccg cggctgtcgc ggaccagatg
      421 gaccaggcgc aggtcgaccc cgcgcatgcg ccggaccagg aaggcgtggg aggcgtgctt
      481 gctggcgtcg accaccagcg gccgcccgct gacctcccgg acggcgtcgt acagccgccc
      541 cagcagctcc aggtagcggc gcatgcgggc ccggtaggcg ggcgacaggg ccggcgcgac
      601 catcagcggc acgaaccggt tgcggtccac cgaggccttg agggccagca cctccccgac
      661 gtcaaggcgg tcccagccac cgaaggcgac ctccccgacc cggccccaga aggcgcactc
      721 ccggaagcgt tcgccgcagc cgcagcggtt gttctccttg aggccgcgct cccagaggtg
      781 gaccagctcc cccaccgagc agacgtcgtc gaggcggccc agcatgcgct ccagcagagt
      841 gctgccgctg cgcccgaggc cgccgatgaa caggaccgtg ctcatcgggc gcccgcccgg
      901 gggcccggcc ggtcgccgac caggccgtcg accaggcggc cgaactcgcg cacggcggtg
      961 gccgtggccc ggcccccggg cccggcccgg aacgcggccg gctcgccggc cacccggtcg
     1021 agcaggcggt gcaggtcggc ctcggtctcg gccaggtgga tgccgccgcc ctcggcggcc
     1081 aggcggcgcg agaaggcgac ctggtggtcg tcgacgtgct cgcccagccg gtgctggcgg
     1141 gggacgacga tcgggacggc gcccatgtgc cgcgccccca ggatggtccc gggcccgccg
     1201 tggcagacga ccgcggccgc gccggccatc gccgcctgga gggcgtcgaa ctcgaggtag
     1261 gcctgccagc tcgcctgccc ggtgggcggg gccgaggtgc cggtctgcat caggcagcgc
     1321 agcgagcccg ggtgggcggc cagccagccg tcggcccagc ggaccaggcg gtcgaacggg
     1381 tggtggtcgg tgccgacggt gaccagcagc agcggctgct ccctgttggt cacagcaggc
     1441 tcccgatgac tttgcccttt gggtagttgc ggcgctgctc ctcccactgg agcaggaaca
     1501 ggtcgctgaa ggggtagcag agccggccgg tcatggtcgg cgagtcgatc cggtcgtaga
     1561 cctcgacgta caccgtccgc ttgccgagca gcttggccag caggaagaac gggaaggcga
     1621 ccccggcgcc gctggacacg accacgtcgg gccggtagga gcgcagcagc ttccaggcca
     1681 ggcgggtgtt gcggagcagg ttggccaggt tgcgggtcgt cgggtggtag gcccaggcga
     1741 cctgctcacc ggcgagcagc gaggtgctgt ccagcttctc gaaggtcacc catagacgat
     1801 cgtgccgttc ccaccatggc ttgaggttgt ggagctggat gaggtggccg ccggaggagc
     1861 agacgaggag ggcgcgcatg gcggcctcag tacgcgcccc tggtcgagac caggagcggg
     1921 acggtcttgg acaggatgaa caggtcgtag gcgatcgacc agttctcgac gtagaacagg
     1981 tcgagccgca cgtagtcctc gaaggagagc tcgctgcgcc cgctgacctg ccacaggccg
     2041 gtgatgccgg g
//
LOCUS       DASSWR010000427         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_383905,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000427 DASSWR010000000
VERSION     DASSWR010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383905"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="VFO47_07120"
     CDS             complement(<1..93)
                     /locus_tag="VFO47_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET8788502.1"
                     /translation="MAHKKGASSSRNGRDSAAKRLGVKRHGGQLV"
     gene            complement(191..484)
                     /gene="rplU"
                     /locus_tag="VFO47_07125"
                     /pseudo
     CDS             complement(191..484)
                     /gene="rplU"
                     /locus_tag="VFO47_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005389206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            619..828
                     /locus_tag="VFO47_07130"
     CDS             619..828
                     /locus_tag="VFO47_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET8788503.1"
                     /translation="MDPTRKLYRSRTNKQVAGVCGGLAAYFNVDVTLMRVLFILLGLL
                     GAAGIVLYIAMWIIVPLEPPVGGAP"
     gene            867..>2325
                     /locus_tag="VFO47_07135"
     CDS             867..>2325
                     /locus_tag="VFO47_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HET8788504.1"
                     /translation="MTTKAKQPDDTTTADPADLFALDGDRRWLLAVEGWAPEREPGIE
                     GALALVNGYLGTRAAVEEGSGASAPATFLNGVFDATPLAAAEAAATPERPVAVAPTTE
                     LVVAPDWSRLRLVAGGVPLTLETAEVLGHRRTLDLRRGVLVREWRLRAGGRTTRLRSL
                     RFASLDDRHILGQALELVAEDWSGPVTVETVVDGDVTNENGVRHLVGHRTGRVDGGLL
                     LETRTADQGVGVCLATSTTLIADDGGEVPAEDTPAERALVRRHRFEAAPGRAWRLHKL
                     VTVFTGRDDPDPAARAAERLRQVAAAGLPAALERSAAAWAGRWAAADVEITGDGELQR
                     DVRFALYHLIGSANPGDPRAAPGARSLTGEKYKGHVFWDTDTFVLPFFVHTHPPTARA
                     MLAYRHHTLPAAREIAAAAGWRGAAYAWESADGGQDVTPAWYITSAGERRPILTGEQE
                     HHLGADIGFAAWQYWTATGDEAFLLDEGAELLFELA"
BASE COUNT          293 a          844 c          864 g          324 t
ORIGIN      
        1 caccagctgg ccgccgtggc gcttgacccc gagccgcttg gcggccgagt cgcggccgtt
       61 gcggctggag gatgcgccct tcttgtgcgc catctcagcc ttcctgttcc tgcttgtcct
      121 gctgctcctg cttggccttg ctgccgccgg accgggaggc gcgcttgcgg gcgccgaggt
      181 cgatcgtctc gaccttgagc ctggtcaggg actggcggtg gccgagggtg cggcgctggc
      241 gggtcttgtt cttgtaggtg aagacgcgga tcttcctgcc ccggtggtgg tcgaccacgc
      301 tggccgtgac cttggccttg gccaggtcgg cggcggccga ggtcacgttg ccgtcgtcgt
      361 cgacgaccag cagcggctcg agctcgaggc tggcaccgtc gtcgccggcg agcttctcga
      421 cgtcgatgag gtcaccgacc ttcaccttgt actgcttccc gccggtccgg atgacggcgt
      481 acacctggga tcgtcctttc aagaagggga cggcgtccgg cagggccgcc agagcgccga
      541 ccgcagaggt tagcaaattt gccggagtcg gtgcgagcct aggtcctgaa caccggacgt
      601 ctcaggaaag gcggcaccat ggacccaacc cggaagctgt accgcagcag gaccaacaag
      661 caggtggccg gggtgtgcgg tggcctggcc gcgtacttca acgtcgacgt gaccctgatg
      721 cgggtgctgt tcatcctgct ggggctcctc ggcgccgcgg gcatcgtgct ctacatcgcc
      781 atgtggatca tcgtccctct ggaaccaccg gtgggcggcg ccccctagga gcaccctacc
      841 ggggccgccg aggttaggct tcgttcatga caaccaaggc gaaacaacct gacgacacga
      901 cgacggccga cccggcggac ctgttcgcgc tggacgggga caggcgctgg ctgctggcgg
      961 tggaggggtg ggcgcccgag cgggagccgg ggatcgaggg ggcgctggcg ctggtcaacg
     1021 gctacctcgg cacgcgggcg gcggtcgagg agggcagcgg cgcctcggcc ccggccacct
     1081 tcctcaacgg tgtcttcgac gccaccccgc tggcggcggc ggaggccgcc gccacccccg
     1141 agcgcccggt ggccgtggcc ccgacgaccg agctggtggt ggcgccggac tggtcgaggc
     1201 tgcggctcgt ggccggcggc gtgccgctga ccctggagac cgccgaggtc ctcggccacc
     1261 gccgcaccct cgacctgcgg cggggcgtgc tggtgcggga gtggcggctg cgcgcaggcg
     1321 gccgcaccac gcggctgcgg tcgctgcggt tcgccagcct cgacgaccgc cacatcctcg
     1381 gccaggcgct ggagctggtg gccgaggact ggtccgggcc ggtcacggtc gagaccgtcg
     1441 tggacggcga cgtgaccaac gagaacggcg tccgccacct cgtcggccac cggaccgggc
     1501 gggtcgacgg cggcctgctg ctggagaccc gcaccgccga ccagggcgtc ggcgtctgcc
     1561 tggccacctc gaccacgctc atcgccgacg acggcggcga ggtgccggcc gaggacacgc
     1621 ccgccgagcg ggcgctggtg cgccgccacc gcttcgaggc ggcgcccggg cgggcgtggc
     1681 ggctccacaa gctggtgacg gtgttcaccg gccgcgacga ccccgacccg gccgcccggg
     1741 ctgccgagcg gctgcgccag gtggccgccg ccgggctgcc cgcggccctg gagcggagcg
     1801 cggccgcctg ggccggacgc tgggcggccg ccgatgtcga gatcaccggg gacggcgagc
     1861 tccagcgcga cgtgcggttc gccctctacc acctgatcgg gagcgccaac cccggcgacc
     1921 cgcgggccgc cccgggggcg cggtcgctca ccggcgagaa gtacaagggc cacgtgttct
     1981 gggacaccga cacgttcgtg ctgcccttct tcgtccacac ccacccgccg acggcccggg
     2041 ccatgctcgc ctaccgccac cacaccctgc cggcggcccg ggagatcgcc gccgcggccg
     2101 gctggcgcgg ggccgcctac gcctgggagt cggccgacgg cgggcaggac gtcaccccgg
     2161 cctggtacat caccagcgcc ggcgagcgcc ggcccatcct caccggcgag caggagcacc
     2221 acctcggcgc cgacatcggc ttcgccgcct ggcagtactg gaccgccacc ggcgacgagg
     2281 cgttcctgct cgacgagggc gcggagctgt tgttcgagct ggccc
//
LOCUS       DASSWR010000428         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1202503, whole genome shotgun sequence.
ACCESSION   DASSWR010000428 DASSWR010000000
VERSION     DASSWR010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202503"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VFO47_07140"
     CDS             <1..1977
                     /locus_tag="VFO47_07140"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET8788505.1"
                     /translation="GEIFGEFEGNLDPTLTQGSGDVKYHLGAVGKHVARSGREVRVEV
                     ASNPSHLEAVNPVVEGIARAKQDLLDRGEEAPVLPILIHGDAAFAGQGVVAETLNLSQ
                     LRGYRTGGTVHILVNNQVGFTTSPDYGRSSTYATDVAKMIQAPIFHVNGDDPEACVRV
                     ARLAFEFRQAFKKDVVIDMWCYRRYGHNEADEPSFTQPLMYERIKSRRPVRKLYTEAL
                     VNRGDLSIEDAERSLEEFRQRLQQAFDDTHDEADHQVAKVELERPAPMAADPAVDTTV
                     DRATLDQLLVKLTTVPDDFNVHPKLTRWLEQRAGALERNAVDWALGEALAFGSLLEEG
                     RTIRLSGQDTRRGTFSQRHSVLVDQRTGDQYSPLANLGEGQGKFFVYDSLLSEFAAMG
                     FEYGYSVANPDALVLWEAQFGDFVNGAQVIVDQFISAAEDKWGQRSSLVLLLPHGFEG
                     QGPEHSSARLERFLDLAAEDNMQVAYPSTPAQYFHLLRRQALRANRKPLVVMTPKSLL
                     RLPAARSTAEQLTTGQFHEVLPDPAEPSPDKVTRVLLCAGKVFYELAKRRADEDLDQL
                     AILRVEQLYPFPARALRAQLDAYPGAEVVAWVQEEPENMGASRYVRLKAEQELGLRLE
                     RVSRDEGAAPAAGSPTLHQQEQRELLDRAFAGLR"
     gene            2013..2384
                     /locus_tag="VFO47_07145"
     CDS             2013..2384
                     /locus_tag="VFO47_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8788506.1"
                     /translation="MKVRDVASSAVVAVGPAQSLREATQLMAKHRVGSAVVQDAEMLI
                     GILTERDVLNAVAHADDPEAVSVEQIMTADVVTVGPDWDLIEAAKVMASRRIRHLVVY
                     EGGQLLGVLSVRDVLPAVLPE"
BASE COUNT          371 a          889 c          832 g          310 t
ORIGIN      
        1 ggggagatct tcggcgagtt cgagggcaac ctcgacccga ccctcaccca gggctcgggc
       61 gacgtgaagt accacctggg cgccgtcggc aagcacgtgg cccgctccgg cagggaggtg
      121 cgggtcgagg tcgcctccaa ccccagccac ctggaggcgg tcaacccggt ggtcgagggc
      181 atcgcccggg ccaagcagga cctgctcgac cggggcgagg aggccccggt gctgccgatc
      241 ctgatccacg gcgacgccgc cttcgccggc cagggggtgg tggccgagac cctcaacctg
      301 tcccagctgc gcggctaccg caccggcggc accgtccaca tcctggtcaa caaccaggtc
      361 gggttcacca ccagccccga ctacggccgc tcctcgacct acgccaccga cgtggccaag
      421 atgatccagg cccccatctt ccacgtgaac ggcgacgacc ccgaggcgtg cgtgcgcgtg
      481 gcccgcctcg ccttcgagtt ccgccaggcg ttcaagaagg acgtcgtcat cgacatgtgg
      541 tgctaccggc gctacggcca caacgaggcc gacgagccgt cgttcaccca gccgctgatg
      601 tacgagcgga tcaagagccg ccggccggtg cgcaagctct acaccgaggc cctggtcaac
      661 cggggcgacc tgtcgatcga ggacgccgag cggtcgctgg aggagttccg ccagcgcctc
      721 cagcaggcct tcgacgacac ccacgacgag gcggaccacc aggtcgccaa ggtcgagctg
      781 gagcggccgg ccccgatggc cgccgacccg gcggtggaca cgaccgtcga ccgcgccacc
      841 ctcgaccagc tcctggtcaa gctgaccacg gttcccgacg acttcaacgt ccaccccaag
      901 ctgaccaggt ggctggagca gcgggccggg gcgctggagc gcaacgccgt cgactgggcc
      961 ctaggcgagg ccctggcctt cgggtcgctg ctcgaggagg gccgcaccat ccggctgtcc
     1021 gggcaggaca cccgccgggg caccttcagc cagcgccact cggtcctggt cgaccagcgg
     1081 accggcgacc agtactcgcc gctggccaac ctgggcgagg gccagggcaa gttcttcgtc
     1141 tacgacagcc tgctcagcga gttcgcggcc atgggcttcg agtacggcta ctcggtggcc
     1201 aaccccgacg cgctggtgct gtgggaggcc cagttcggcg acttcgtcaa cggcgcccag
     1261 gtcatcgtcg accagttcat ctcggccgcc gaggacaagt ggggccagcg ctccagcctg
     1321 gtcctgctcc tcccccacgg cttcgagggc caggggccgg agcactccag cgcccgcctg
     1381 gagcggttcc tcgacctcgc cgccgaagac aacatgcagg tcgcctaccc ctcgaccccg
     1441 gcccagtact tccacctgct gcgccgccag gccctgcggg ccaaccgcaa gccgctggtg
     1501 gtgatgaccc ccaagtcgct gctgcggctg ccggcggccc gctccacggc cgagcagctc
     1561 accaccggcc agttccacga ggtcctgccc gaccccgccg agccgtcgcc cgacaaggtc
     1621 acccgggtgc tgctctgcgc cggcaaggtc ttctacgagc tggccaagcg gcgagccgac
     1681 gaggacctcg accagctggc catcctccgg gtggagcagc tgtacccgtt cccggccagg
     1741 gcgctgcggg cccagctcga cgcctacccc ggcgccgagg tcgtggcctg ggtccaggag
     1801 gagccggaga acatgggcgc ctcgcgctac gtgcgcctca aggcggagca ggagctcggg
     1861 ctgcggctgg agcgggtctc ccgtgacgag ggcgcggccc cggccgccgg cagccccacc
     1921 ctccaccagc aggagcagcg ggagctactc gatcgcgcgt tcgccgggtt aaggtagcgc
     1981 caaccctgaa ccgcgaccga ggaggtgccg ccatgaaggt gcgggacgtg gcgagcagcg
     2041 cggtggtggc ggtgggcccg gcccagtcgc tccgggaggc gacccagctg atggccaagc
     2101 accgggtggg gtcggccgtg gtccaggacg ccgagatgct gatcggcatc ctcaccgagc
     2161 gggacgtgct caacgcggtc gcgcacgccg acgaccccga ggccgtcagc gtggagcaga
     2221 tcatgaccgc cgacgtggtc accgtcgggc ccgactggga cctgatcgag gcggccaagg
     2281 tgatggccag ccgacgcatc cgccacctgg tggtctacga gggcggccag ctcctcgggg
     2341 tgctgagcgt ccgcgacgtc ctcccggcgg tcctgccgga gtagccggcg gggtggccgc
     2401 gg
//
LOCUS       DASSWR010000429         6810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_355788,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000429 DASSWR010000000
VERSION     DASSWR010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355788"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFO47_07150"
     CDS             <1..721
                     /locus_tag="VFO47_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788507.1"
                     /translation="ARGDGFEVYSTLLASLCPFGRRRDGRLVVRNPLAGLAGIDVAPG
                     LVAVVCILLGGAAFDGLARTRWWASLTGRSGHVDLLLGTAGLAAAIRFVAVTYLAATR
                     ASQRYRRVRVAGDDDRLDRRFVHTLLPIVVGYTLAHYFSLLVLQGQNGYIRASDPFGQ
                     GWDLFGTASWVQHPLAVSTATIALVQVTAIVAGHMVGVVAAHDRAMATFRGRDRLRGQ
                     YLLFATMVCYTVGGIALLVGT"
     gene            718..1563
                     /locus_tag="VFO47_07155"
     CDS             718..1563
                     /locus_tag="VFO47_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014176815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788508.1"
                     /translation="MRGTAPVNGVRLHYVVEGDGPLVVLLHGWPQTWYCWREVIAPLA
                     AAHTVLAPDLRGYGQSDKPPAGYDKRTMATDVRALAAHLGHSRIALLAGHDRGARVAH
                     RYALDHPDEVERLAVLDVVPTREMFSRPDATIARGYWHWLFHLQPDLPELLVGPNIEA
                     YLRWFFERWTTNRPAVEAAVPEYVRAFEAPGALRAGFDDYRATFPDDLDADEASAAAG
                     QRLSMPVLALWGDAGLPSRLPVLDIWRRYADDVRGQAVAGCGHFLPEEQPDEVAAQLL
                     AFLGG"
     gene            1584..2837
                     /locus_tag="VFO47_07160"
     CDS             1584..2837
                     /locus_tag="VFO47_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788509.1"
                     /translation="MTPLQRLRRAAIDLRPLRHTDFRRLWVGNSLGFVGFQLTAVAVP
                     VQVYELTGSSFWVGMLGLVGLVPLVLFGLWGGAVADAVDRRVLLLGSSLLLWACTGGL
                     LAAELAGVASLPLLLGLVAVQAAGFAVASSTRGAIVPRVLPERLVPAANTLSFTASNV
                     GLVLGPLLAGLILARWSFAAAYAADLAVFTVALYAAARLPALPPAGPATSPGLRSVAE
                     GLAFIATRPVLLLSFAVDIAAMVLAMPRALFPETAATLGGRGAVGWLYAAIAMGAVAA
                     GLSSGWIGRVGRQGVALVAAVVAWGLAVAAAGLAPALWMTVALLAVAGAADLVSAVYR
                     QTILQTYAPDEMRGRMQGVFIVVVAGGPRLGDLRAGATAAAFGATVSWVGGGLVCAAL
                     VALALLVPALRNYDAHAPRVENGVS"
     gene            2834..4099
                     /locus_tag="VFO47_07165"
     CDS             2834..4099
                     /locus_tag="VFO47_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HET8788510.1"
                     /translation="MTAPRLRDTDLDALDRRLAPADAELAARYPGDSGARQPVHTVYV
                     PADQMTPGLPAGWGAQALAALEEHAAAAPALAEAAGLDPALVGRVHGGVLAKLEREPI
                     EDLRLDFEDGYGPRPPAEEDGHALGAARALAGALAGAAPPPYVGLRVKCLEAATRRRG
                     LRTLDLFLGALLAETGLPDGFVVTLPKVTDVRQVEAMALVCGLLEEAHGLGDGRLRFE
                     IQVETPQSILAADGTATVARLVHAAGGRCSGLHYGTYDYSAACGIAAAYQSMEHPAAD
                     HAKAVMQVAAAGTGVRLSDGSTNVLPVGDRAAVHAAWRLHARLVRRSLERGYWQGWDL
                     HPAQLPTRYLATYAFFREGLPASAARLRAYAERTGGGVLDEPATAQALASFVARGLDC
                     GALTEAEVRELTGLDRAAVDGYARRSGSR"
     gene            4178..6112
                     /gene="uraD"
                     /locus_tag="VFO47_07170"
     CDS             4178..6112
                     /gene="uraD"
                     /locus_tag="VFO47_07170"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET8788511.1"
                     /translation="MARTDERDQQASGRGAVHPVDEVLPVPRLAVYGFQHVLAFYAGA
                     VIVPILLASAIDLTPEQLAYLINADLFTCGIASIIQSVGFWKIGVRLPLLQGVTFAAV
                     SPMIAIGLAEGGGVPGLKAIYGSVIVAGIFAFLVAPFFARLIRLFPPIVTGTVITIIG
                     IVLLSVAALDAGGGGASQFTDPPTFGALRNLALAGFTLLIILVIYRMFTGFVATVAVL
                     IGLVVGTLVGWLFDFTDFSRVAGADVLGVTTPFYFGAPTFNAAAIISMVIVMLITMVE
                     TTGDVFAAGEIVGKPVDKEDVARALRADGLATTLGGILNSFPYTCFAENVGLVRLTKV
                     KSRWVVATAGLFMILIGLVPKVGAIVAAIPPPVLGGAGFALFGTVAVIGIQTLRRVDF
                     HDERNVIILAVSLGFAMTPTVYPTITSFFPEAVRTIISSGITLGSISAILLNLVFNVW
                     GGRSNLVTRVLPVPRHEDVLSIDQVNQMDREQFVATFGPLFQGAAWISEQAFDAHPFE
                     NVYDLRRAFHDALFDAPPERQLELIRQYPDLAGAATREGRLPTQSVVDQAIAGLDRLS
                     SDEYTSFDELNRAYREKFGFPLVVAVRENTKETILKSGNARLQNSPAAEQAAALVEIA
                     KIANLRLLDIVEEPVDQPVA"
     gene            6113..>6810
                     /locus_tag="VFO47_07175"
     CDS             6113..>6810
                     /locus_tag="VFO47_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8788512.1"
                     /translation="MGRLVLRGARWPGDVAVEGGRIAAVGSVPDQPGDEVVRVDGDIV
                     TAGLVNTHHHFYQWMTRGWAFDRTLFGWLTTLYPVWAKLTPEDVETASAVAMGELALT
                     GCTTAADHHYLVPGGDDSVFDAIASAARTIGIRVHIARGSMDLGQSKGGLPPDSVVED
                     LDAILASTQAVHERLHDGDRVVVTVAPCSPFSVTPELMTESAALARRLGLRLHTHLAE
                     TLDEERSCLERFGRR"
BASE COUNT          770 a         2643 c         2396 g         1001 t
ORIGIN      
        1 cgcccggggc gacgggttcg aggtctactc gaccctgctc gcctccctct gcccgttcgg
       61 ccggcgccgc gacggccgcc tggtggtccg caacccgttg gccgggctgg ccggcatcga
      121 cgtcgccccc gggctggtgg cggtggtctg catcctgctc ggcggggccg ccttcgacgg
      181 gctggcccgg acgcgctggt gggcgtcgct gaccggccgc agcggccacg tcgacctgct
      241 cctgggcacg gccgggctgg ccgcggccat ccggttcgta gccgtcacct acctggccgc
      301 cacccgggcc agtcagcgct accgccgggt ccgggtggcc ggcgacgacg accgcctcga
      361 ccggcggttc gtgcacaccc tgctgccgat cgtcgtcggc tacaccctgg cccactactt
      421 ctccctgctg gtgctgcagg gccagaacgg ctacatccgg gcctcggacc ccttcgggca
      481 gggctgggac ctgttcggca cggccagctg ggtccagcat ccccttgccg tgtccaccgc
      541 cacgatcgcc cttgtccagg tcaccgccat cgtcgccggc cacatggtcg gggtggtggc
      601 cgcccacgac cgggccatgg ccaccttccg cggccgcgac cggctgcggg gccagtacct
      661 cctgttcgcc accatggtct gctacaccgt cgggggcatc gccctgctgg tggggacatg
      721 aggggcacgg cgccggtcaa cggcgtgcgg ctccactacg tggtcgaggg cgacggcccg
      781 ctggtcgtgc tgctgcacgg ctggccccag acctggtact gctggcgcga ggtgatcgcc
      841 cccctggccg ccgcccacac cgtgctcgcc cccgacctgc gcggctacgg gcagagcgac
      901 aagcccccgg ccggctacga caagcggacc atggccaccg acgtccgcgc cctggccgcc
      961 cacctgggcc actcgcggat cgccctgctg gccggccacg accgcggggc cagggtcgcc
     1021 caccgctacg ccctcgacca cccggacgag gtcgagcggc tggccgtgct cgacgtcgtc
     1081 cccacccgcg agatgttcag ccggcccgac gccaccatcg cccgcggcta ctggcactgg
     1141 ctgttccacc tccagcccga cctccccgag ctcctggtcg ggccgaacat cgaggcctac
     1201 ctgcgctggt tcttcgagcg ctggacgacc aaccgcccgg ccgtcgaggc ggccgtgccc
     1261 gagtacgtgc gcgcctttga ggccccgggc gcgctgcggg ccggcttcga cgactaccgg
     1321 gccaccttcc ccgacgacct ggacgccgac gaggccagcg ccgccgccgg ccagcgcctg
     1381 tccatgcccg tgctggccct gtggggcgac gccggcctgc ccagccgcct ccccgtgctc
     1441 gacatctggc gccgctacgc cgacgacgtc cgcggccagg ccgtcgccgg ctgcggccac
     1501 ttcctccccg aggagcagcc ggacgaggtc gccgcccagc tgctcgcctt cctcggcggc
     1561 tgacctccct atcctgggag gccatgacgc cactgcagcg gctgcggcgg gccgcgatcg
     1621 acctccggcc gctgcggcac accgacttcc gtcggctgtg ggtggggaac tcgctcgggt
     1681 tcgtggggtt ccagctgacc gcggtggccg tgccggtgca ggtgtacgag ctgaccgggt
     1741 cctcgttctg ggtggggatg ctggggctgg tggggctggt cccgctggtg ctgttcgggc
     1801 tctggggcgg ggcggtggcc gacgccgtcg accgccgggt gctgctgctg gggtcgtcgc
     1861 tgctgctgtg ggcctgcacc ggcgggctgc tggcggcgga gctggccggc gtggccagcc
     1921 tgcccctgct gctggggctg gtcgccgtcc aggcggccgg gttcgcggtc gcctcctcga
     1981 cccggggggc gatcgtcccc cgggtcctgc ccgagcggct ggtgccggcg gccaacaccc
     2041 tgtcgttcac ggcctccaac gtcggcctgg tcctcggccc cctgctggcc gggctgatcc
     2101 tggcccgctg gtcgttcgcg gccgcctacg ccgccgacct ggccgtgttc accgtcgccc
     2161 tgtacgcggc ggcgcggctg ccggccctcc ccccggccgg gccggccacc agcccggggc
     2221 tgcggtcggt ggccgaaggg ctggccttca tcgccacccg gccggtgctg ctgctgtcgt
     2281 tcgccgtcga catcgcggcc atggtgctgg ccatgccccg ggccctgttc cccgagaccg
     2341 cggccaccct cggcggccgc ggggccgtgg gctggctgta cgcggccatc gccatggggg
     2401 cggtggcggc cgggctgtcc tcgggctgga tcgggcgggt cggccgccag ggcgtcgccc
     2461 tggtcgcggc cgtggtcgcc tgggggctgg ccgtggccgc ggccgggctg gccccggcgc
     2521 tctggatgac cgtggccctg ctggccgtgg ccggcgcggc cgacctggtc tcggccgtct
     2581 accggcagac gatcctccag acctacgctc ccgacgagat gcgcgggcgc atgcaggggg
     2641 tgttcatcgt ggtcgtggcc ggcggccccc gcctcggcga cctgcgggcg ggggccaccg
     2701 cggccgcctt cggggccacc gtgtcctggg tcgggggcgg gctcgtctgc gccgccctgg
     2761 tcgccctggc cctgctcgtg cccgccctgc ggaactacga cgcccacgcc ccccgggtgg
     2821 agaatggggt ctcatgaccg ctcctcgcct gcgcgacacc gacctggacg cgctcgaccg
     2881 gcgcctcgcc ccggccgacg ccgagctcgc cgcccgctac ccgggggact ccggggcccg
     2941 gcagccggtc cacaccgtct acgtcccggc cgaccagatg acccccggcc tgcccgccgg
     3001 ctggggggcg caggccctgg ccgccctgga ggagcacgcc gccgccgccc ccgccctggc
     3061 cgaggccgcc gggctcgacc cggccctggt cgggcgggtg cacggtggcg tgctggccaa
     3121 gctggagcgg gagccgatcg aggacctgcg cctcgacttc gaggacggct acgggccgcg
     3181 gccgccggcc gaggaggacg gccacgccct cggcgccgcc cgggcgctgg ccggcgcgct
     3241 cgccggggcc gcgcccccgc cgtacgtggg cctgcgcgtc aagtgcctgg aggcggcgac
     3301 ccggcgccgg gggctgcgga ccctcgacct gttcctgggg gcgctgctgg ccgagaccgg
     3361 gctcccggac gggttcgtgg tcaccctgcc caaggtgacc gacgtgcgcc aggtggaggc
     3421 catggccctg gtctgcgggc tgctggagga ggcgcacggg ctgggcgacg ggcggctgcg
     3481 gttcgagatc caggtcgaga ccccgcagtc gatcctggcc gccgacggca cggccaccgt
     3541 ggcccggctc gtccacgccg ccggcggccg ctgctcgggc ctccactacg gcacctacga
     3601 ctacagcgcc gcctgcggga tcgccgccgc ctaccagagc atggagcacc cggcggccga
     3661 ccacgccaag gcggtcatgc aggtggcggc cgccggcacc ggggtgcgcc tgtccgacgg
     3721 gtccaccaac gtgctcccgg tgggcgaccg ggcggccgtc cacgccgcct ggcggctgca
     3781 cgcccgcctg gtccggcgct cgctcgagcg cggctactgg cagggctggg acctgcaccc
     3841 ggcccagctc cccacccgct acctggccac ctacgccttc ttccgtgagg gcctgcccgc
     3901 gtcggcggcc cggctgcggg cctacgccga gcggaccggc ggcggggtcc tggacgagcc
     3961 ggccaccgcc caggctttgg cctccttcgt ggcccgcggc ctcgactgcg gcgccctgac
     4021 cgaggccgag gtgcgggagc tgaccggcct ggaccgggcc gcggtggacg gctacgccag
     4081 gcgttccggg tcccgctgag gccttgacgg ctgcggatac tcgtagtgga atagttatct
     4141 cgcatgatgg aaatcgggtg aaaggctggt gcacgacatg gccaggacag acgagcgcga
     4201 ccaacaggca tccggccgtg gcgccgtcca cccggtggac gaggtcctcc cggttccgag
     4261 actggccgtc tacgggttcc agcacgtgct ggccttctac gccggcgccg tcatcgtgcc
     4321 catcctgctc gccagcgcca tcgacctcac ccccgagcag ctcgcctacc tgatcaacgc
     4381 cgacctgttc acctgcggca tcgcctcgat catccagtcg gtcggcttct ggaagatcgg
     4441 ggtgcggctg cccctgctcc agggggtcac cttcgccgcc gtctccccga tgatcgccat
     4501 cggcctggcc gagggcggcg gcgtgcccgg cctcaaggcc atctacggct cggtgatcgt
     4561 cgccgggatc ttcgccttcc tggtcgcgcc cttcttcgcc aggctgatcc ggctgttccc
     4621 gccgatcgtc accggcaccg tcatcaccat catcggcatc gtgctgctgt cggtggccgc
     4681 cctggacgcc ggcggcggcg gggccagcca gttcaccgac ccgccaacct tcggggcgct
     4741 ccggaacctg gccctggccg gcttcaccct gctgatcatc ctggtcatct accggatgtt
     4801 caccggcttc gtggccaccg tcgccgtgct catcggcctg gtcgtcggca ccctggtcgg
     4861 ctggctgttc gacttcaccg acttcagccg ggtcgccggg gccgacgtgc tcggggtcac
     4921 cacccccttc tacttcggcg cccccacctt caacgcggcc gcgatcatct cgatggtgat
     4981 cgtgatgctg atcaccatgg tcgagaccac cggcgacgtg ttcgccgccg gcgagatcgt
     5041 cggcaagccg gtggacaagg aggacgtggc cagagcgctg cgggccgacg gcctggccac
     5101 caccctcggc ggcatcctca actccttccc ctacacctgc ttcgccgaga acgtcgggct
     5161 ggtccggctg accaaggtca agagccgctg ggtggtggcc accgccgggc tgttcatgat
     5221 cctcatcggg ctggtcccca aggtcggggc catcgtcgcc gccatcccgc cgccggtgct
     5281 cggcggggcc gggttcgccc tgttcggcac cgtggccgtg atcggcatcc agaccctgcg
     5341 ccgggtcgac ttccacgacg agcgcaacgt gatcatcctg gccgtcagcc tcggcttcgc
     5401 catgacgccg acggtctacc cgacgatcac cagcttcttc cccgaggcgg tgcggacgat
     5461 catctccagc ggcatcaccc tcggcagcat cagcgcgatc ctgctcaacc tggtcttcaa
     5521 cgtctggggg ggcaggagca acctggtcac ccgggtgctg ccggtccccc gccacgagga
     5581 cgtcctcagc atcgaccagg tcaaccagat ggaccgggag cagttcgtgg ccaccttcgg
     5641 gcccctgttc cagggcgccg cctggatctc cgagcaggcc ttcgacgccc accccttcga
     5701 gaacgtctac gacctgcgcc gggccttcca cgacgccctg ttcgacgccc cgcccgagcg
     5761 ccagctggag ctgatccgcc agtacccgga cctggccggc gcggccaccc gcgagggccg
     5821 gctgcccacc cagtcggtgg tcgaccaggc gatcgccggc ctggaccggc tcagcagcga
     5881 cgagtacacc tccttcgacg agctcaaccg cgcctaccgg gagaagttcg gcttcccgct
     5941 ggtggtggcc gtgcgcgaga acaccaagga gaccatcctc aagtcgggca acgcccggct
     6001 gcagaactcc cccgccgccg agcaggcggc cgccctggtc gagatcgcca agatcgccaa
     6061 cctgcgcctg ctcgacatcg tcgaggagcc ggtcgaccag ccggtggcct gattgggccg
     6121 cctggtcctg cggggagcgc gctggccggg ggacgtggcc gtcgagggcg gccggatcgc
     6181 cgccgtgggg tcggtgccgg accagcccgg cgacgaggtc gtacgggtcg acggcgacat
     6241 cgtcaccgcc gggctggtca acacccacca ccacttctac cagtggatga cccgcggctg
     6301 ggcgttcgac cgcaccctgt tcggctggct gaccaccctg tacccggtgt gggccaagct
     6361 gacccccgag gacgtggaga cggcctcggc cgtggccatg ggcgagctcg ccctgaccgg
     6421 ctgcaccacc gccgccgacc accactacct ggtcccgggc ggcgacgact cggtgttcga
     6481 cgccatcgcc tcggcggcca ggaccatcgg aatccgggtc cacatcgccc gcggctccat
     6541 ggacctcggc cagagcaagg gcggcctgcc gccggactcg gtggtcgagg acctggacgc
     6601 catcctggcc tcgacccagg cggtgcacga gcggctccac gacggcgacc gggtcgtggt
     6661 caccgtcgcc ccctgcagcc ccttcagcgt caccccggag ctgatgaccg agtcggcggc
     6721 cctggccagg cgcctggggc tgcggctgca cacccacctg gccgagaccc tggacgagga
     6781 gcggagctgc ctggagcggt tcggccggcg
//
LOCUS       DASSWR010000430         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_214402,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000430 DASSWR010000000
VERSION     DASSWR010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214402"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFO47_07180"
     CDS             complement(<1..285)
                     /locus_tag="VFO47_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788513.1"
                     /translation="MTSVRVPGFLPSTRGLHFPNAFPHVPNVEIDLPGGMTIPIGDAA
                     NGLCGGMAYTVRDLFEAGLAPPAVTRAPGDGPLFRYVADRLLDSLSLPFGP"
     gene            complement(399..644)
                     /locus_tag="VFO47_07185"
     CDS             complement(399..644)
                     /locus_tag="VFO47_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788514.1"
                     /translation="MNRFEDAIARELRPRLRARTRSVRALARWAAASVPPGALVADGV
                     ALVLDRLGDGDPDELAAQVMAETPDRFGRNWESGPAH"
     gene            694..960
                     /locus_tag="VFO47_07190"
     CDS             694..960
                     /locus_tag="VFO47_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788515.1"
                     /translation="MRPPWRFRGEPLALEWVADGWHLRFVQPYRATKVYRCPGCQQEI
                     FPRTLHVVVWPEGEPAQRRHWHKACWERRFAELQRAARRGRPAT"
     gene            1021..1626
                     /locus_tag="VFO47_07195"
     CDS             1021..1626
                     /locus_tag="VFO47_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET8788516.1"
                     /translation="MSVSQSALGRPRRLVLADVLPGDRVRDAVLVAGFAAFTGLAAQL
                     AVKLPFTPVPITGQTFAVLLGAAALGWRRALAGMVLYLALGLTPWVPWFAEGKGGTDV
                     VQAPSFGYVVGFLVAAVLVGWLAERGWDRTPARTVVTMVAGNLVIYAFGLPWLMATVD
                     VGLATGLELGVTPFLAGDALKILLAAGLLPGAWALVGRDRE"
     gene            1630..2370
                     /locus_tag="VFO47_07200"
     CDS             1630..2370
                     /locus_tag="VFO47_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8788517.1"
                     /translation="MSVEETLRLDGRTALVTGASRGIGRQAALTLAAAGASVVLAARS
                     AEDLAEVAAAARKAGAPDALVAVTDVLDEAAVEGTGRLDVVVNVAGGQGFTAYVADTR
                     TEGWDKVLNLNLRSVFVGCKAAMPHLQAGGSIVNVASIAGFTASPGLAAYGAAKAAVI
                     ALTRTLAVEAAPHGVRVNCLAPGWIRTELTRRMWSDPETSRALVAQIPLGRWADVEEL
                     AGPLLLLASDAGSYITGATLVVDGGLLA"
     gene            2441..3076
                     /locus_tag="VFO47_07205"
     CDS             2441..3076
                     /locus_tag="VFO47_07205"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET8788518.1"
                     /translation="MGDSLLDRVTRLPFVIPGDPTGVPASVLLALAPVEGADDLELVL
                     VQRPGHLRQHAGQVGLPGGAVEPGDADGVAAALREAHEEVGLDPSGVRVLGSLDRAYL
                     AVSDFDVLPVVGVWDGLAPLTPSPDEVAGILRPTLRQLADPASHGSMSFEAIMGEVEV
                     AARNLPGDLSSPVFWVDGTLVWGFTAGLLAGFLDSLGLQAPPLPAYWGVRP"
     gene            3099..3953
                     /locus_tag="VFO47_07210"
     CDS             3099..3953
                     /locus_tag="VFO47_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8788519.1"
                     /translation="MTTAPPDGPEPTPAPEPPEPDPAPAPPVPPAATSGAGTPLVLLH
                     AFPLDGRMWAPQVEALAGTYQVIVPDLRGFGAARDQAVEEAGMDLLADDLARLLDDRG
                     LDRVVLGGLSLGGYVALAFMRRHADRVSGLVLLDTKAAADGDQARDARLEMAERVLAE
                     GNDFVPEVLLPRLLGETSREHRPEVVSKVAALIREQSPAAIAGAQRGMAARADTTDVL
                     ATIKVPTLVVTGEEDAVTGPEVGRDLAAGIPGARFLLVEEAGHLVNLEQPEIVNEALL
                     DFLAPLWI"
     gene            4110..4658
                     /locus_tag="VFO47_07215"
     CDS             4110..4658
                     /locus_tag="VFO47_07215"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HET8788520.1"
                     /translation="MILVAVVVLAALAVPLAGGRLGALVELRLRRVWAIFAALALQVV
                     ALDLPGLPEGARAVLVVASYPVGALFLAANWRVPGMRLVALGGALNLAAICANGGVMP
                     AAPSALAGAGLPPDAPGFQNSAAVADPRLAFLGDVFYVPASWPLSNVFSVGDVLIALG
                     SLWALHRICRSRLAPSWARQHS"
BASE COUNT          525 a         1818 c         1794 g          646 t
ORIGIN      
        1 cggcccgaac ggcagcgaca ggctgtccag cagccggtcg gcgacgtagc ggaacagcgg
       61 gccgtcgccg ggcgccctgg tgacggccgg cggggccagg cccgcctcga acaggtcgcg
      121 gacggtgtag gccatgccgc cgcagaggcc gttggcggcg tcgccgatcg ggatcgtcat
      181 cccgcccggc aggtcgatct cgacgttggg tacatgggga aaggcgttgg ggaagtgcag
      241 gccccgggtc gacgggagga acccggggac gcggacgctg gtcatgcggt cctccgtgcg
      301 atgggctcgg ttcgcccccc ggccagagca tggcccggga ccggcctccg gcccatgacg
      361 gccgtcaccg ccgggacgtg ccgttcgtca cgtcgcgctc agtgggcggg gcccgactcc
      421 cagttgcggc cgaagcggtc gggggtctcg gccatgacct gggcggccag ctcgtccggg
      481 tcgccgtcgc cgaggcggtc aaggaccagc gccaccccgt cggcgaccag cgccccgggc
      541 ggcaccgagg ccgcggccca gcgggccagg gcccggacgc tgcgggtcct ggcccgcagg
      601 cggggccgca gctcgcgggc gatggcgtcc tcgaagcggt tcatcggcgg cgacgagcct
      661 acgccggttg cgtacactcg ggccgtgagc acgatgcggc cgccgtggcg gttccggggc
      721 gagccgctgg ccctggaatg ggtcgccgac ggctggcacc tgcgcttcgt gcagccgtac
      781 cgggcgacca aggtgtaccg ctgccccggc tgccagcagg agatcttccc ccgcaccctc
      841 cacgtggtgg tgtggccgga gggcgagccc gcccagcgcc gccactggca caaggcgtgc
      901 tgggagcggc gcttcgccga gctgcagcgg gcggcgcggc gtggccgccc ggcgacgtag
      961 agttgggcgt ccgggcaacg ggacccgggc accgccgcag gaacggaaaa ggagccacca
     1021 gtgtccgtgt cgcagagcgc tctcggccgt ccccgccgcc tcgtgctcgc cgacgtgctg
     1081 ccaggcgacc gcgtccgcga cgccgtcctg gtggccgggt tcgccgcctt caccggcctg
     1141 gccgcccagc tcgccgtcaa gctgccgttc acgccggtgc cgatcaccgg gcagaccttc
     1201 gccgtgctgc tgggcgcggc cgccctcggc tggcgccgcg ccctggccgg gatggtgctg
     1261 tacctggccc tcgggctgac cccgtgggtg ccctggttcg ccgagggcaa gggcggcacc
     1321 gacgtggtcc aggcgccctc gttcggctac gtcgtcgggt tcctggtcgc ggccgtcctg
     1381 gtcgggtggc tggccgagcg gggctgggac cggaccccgg cgcgcacggt cgtgaccatg
     1441 gtcgccggca acctggtcat ctacgccttc gggctgccct ggctgatggc caccgtcgac
     1501 gtcggcctgg ccacggggct ggagctgggc gtgaccccgt tcctggccgg cgacgccctc
     1561 aagatcctgc tcgccgccgg cctgctcccg ggcgcctggg ccctggtcgg gcgggatcgg
     1621 gaatagcgca tgtcggtcga ggagacgctg cgcctggacg gtcggacggc gctggtcacc
     1681 ggggccagcc gtggtatcgg ccggcaggcc gccctgaccc tggccgccgc cggggcctcg
     1741 gtggtcctgg ccgcccgctc ggccgaggac ctggccgagg tggccgccgc cgcccgcaag
     1801 gccggcgccc ccgacgccct ggtcgcggtc accgacgtcc tcgacgaggc ggccgtcgag
     1861 ggcacgggac ggctcgacgt ggtcgtcaac gtggccggcg gccagggctt caccgcctat
     1921 gtcgccgaca cccggaccga gggctgggac aaggtgctca acctcaacct gcgctcggtg
     1981 ttcgtcggct gcaaggccgc catgccccac ctgcaggcgg gcgggtcgat cgtgaacgtg
     2041 gccagcatcg ccgggttcac tgccagcccc gggctggccg cctacggggc ggccaaggcg
     2101 gccgtgatcg ccctcacccg caccctggcc gtcgaggcgg ccccgcacgg ggtacgggtc
     2161 aactgcctgg cccccggctg gatccggacc gagctgaccc gccgcatgtg gtcggacccg
     2221 gagaccagcc gggccctggt cgcccagatt cccctcggcc gctgggccga cgtcgaggag
     2281 ctggccgggc cgctgctgct gctcgcctcc gacgccggca gctacatcac cggcgcgacc
     2341 ctggtggtcg acggcgggct gctggcgtga gccggagggg tttggggaac cccggggggg
     2401 ttccccaatg agcgggccgg atcggaccta tgctgggcgg atgggcgact cgctgctgga
     2461 ccgggtgacc cggctgccgt tcgtcatccc cggcgacccc accggggtgc cggcgtcggt
     2521 gctgctcgcc ctggccccgg tggagggggc cgacgacctg gagctggtgc tggtgcagcg
     2581 gcccgggcac ctgcgccagc acgccggcca ggtgggcctg cccgggggcg cggtcgagcc
     2641 gggggacgcc gacggggtcg ccgccgccct acgcgaggcc cacgaggagg tcggcctcga
     2701 cccgtccggg gtccgcgtgc tcggcagcct ggaccgggcg tacctggccg tctccgactt
     2761 cgacgtgctg ccggtggtcg gggtctggga cggcctggcc ccgctcaccc cgagcccgga
     2821 cgaggtcgcc gggatcctgc ggccgaccct gcgccagctt gccgacccgg ccagccacgg
     2881 cagcatgtcg ttcgaggcca tcatgggcga ggtggaggtg gccgcccgca acctccccgg
     2941 cgacctgagc agccccgtgt tctgggtcga cgggaccctg gtctgggggt tcacggccgg
     3001 cctgctggcc gggttcctcg actccctcgg cctccaggcc ccgcccctcc ccgcctactg
     3061 gggcgtccgc ccctgaccct gacccctagg agcagctgat gacgaccgcc ccgcccgacg
     3121 gacccgagcc gaccccggcc ccggaaccgc ccgagcccga cccggcgccg gcgccgccgg
     3181 tcccgccggc cgcgaccagc ggcgccggga cgccgctggt cctgctgcac gccttccccc
     3241 tcgacggccg catgtgggcg ccccaggtcg aggccctggc cggcacctac caggtgatcg
     3301 tccccgacct gcgcggcttc ggggcggccc gggaccaggc ggtcgaggag gccggcatgg
     3361 acctgctggc cgacgacctg gcccgcctcc tggacgaccg cggcctggac cgggtggtgc
     3421 ttggcggact gtcccttggc ggctacgtgg ccctggcctt catgcgccgc cacgccgacc
     3481 gggtcagcgg cctggtcctg ctcgacacca aggccgccgc cgacggcgac caggccaggg
     3541 acgcccggct ggagatggcc gagcgggtcc tggccgaggg caacgacttc gtgcccgagg
     3601 tgctgctgcc ccggctgctc ggcgagacca gccgcgagca ccggcccgag gtggtctcca
     3661 aggtcgccgc cctgatccgc gagcagagcc cggcggccat cgccggcgcc cagcggggca
     3721 tggccgcccg cgccgacacc accgacgtgc tggccacgat caaggtcccg accctggtcg
     3781 tgaccggcga ggaggacgcc gtcaccgggc ccgaggtcgg ccgcgacctg gccgccggca
     3841 tccccggggc ccggttcctg ctggtcgagg aggcggggca cctggtcaat ctggagcagc
     3901 cggagatcgt gaacgaggcg ctgctggact tcctggcccc actctggatc tgagcgtgct
     3961 tgctcgatga caactggtcc acccatctag tacgctcggg ctagtcgcgg ctctggttcc
     4021 cccgaccaga gccgaagcgc gaggggggca ggggaggacc ggctcctgcc cccctcgcca
     4081 tgttcccaga gcgcccgcgg agccgccggt tgatcctcgt cgccgtggtc gtcctggccg
     4141 ccctggcggt gccgctggcc ggcgggcggc tgggcgcgct ggtcgagctc cgcctccgcc
     4201 gcgtctgggc catcttcgcc gccctggccc tgcaggtcgt cgccctcgac ctgcccgggc
     4261 tgcccgaggg ggcgcgggcc gtcctggtgg tggcctccta cccggtcggg gcgctgttcc
     4321 tggccgccaa ctggcgggtc ccggggatgc ggctggtcgc cctcgggggg gcgctcaacc
     4381 tggccgccat ctgcgccaac ggcggggtca tgccggccgc gccgtcggcc ctggccgggg
     4441 ccggcctgcc gccggacgcg cccgggttcc agaactcggc cgccgtcgcc gacccgcgcc
     4501 tggccttcct cggcgacgtc ttctacgtcc cggcctcgtg gccgctgagc aacgtcttca
     4561 gcgtcggcga cgtgctgatc gccctcggga gcctctgggc gctgcaccgc atctgccggt
     4621 cacgcctggc cccgtcctgg gcgcgccaac actcgtaaac cggcggtcgc caagctggcc
     4681 cagcacgacc gcgccaggtc ctttgtttga cagttcccac cgtgggatgt ctccccccgg
     4741 catactggcc accaggctcg cccagggact cgtgacgcag ctg
//
LOCUS       DASSWR010000431         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_355828,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000431 DASSWR010000000
VERSION     DASSWR010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355828"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..398
                     /locus_tag="VFO47_07220"
     CDS             45..398
                     /locus_tag="VFO47_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET8788521.1"
                     /translation="MAALLQRLIRARQPGGAALGSGAARNHLNRVVRAFRAPAGHPER
                     AEPAASGLVEPLTKRELEVLGLIAAGKPNQEIADELVVTLATVKKHTSHIFDKFGAAS
                     RTQAVARARELGLLP"
     gene            554..1291
                     /locus_tag="VFO47_07225"
     CDS             554..1291
                     /locus_tag="VFO47_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET8788522.1"
                     /translation="MTQELTTPGASASGAVSETTPTVTRALLVCGVVAGPLYVVVALL
                     QVLFRDGFDLGRHPLSLLSLGELGWIQITNFIVGGLLAVGFAVGLRRVLHPGRGGTWA
                     PLLVGGYGLGLIAGGVFIADPALGFPPGTPDGIPDQFSWHGLLHAFAPPLASLSLIVA
                     CFVLARRFAGLGHRGWATYSAATGVAALALSAWPNQATVSVRLAVAVVIGWTWVSVLA
                     TRLLVEPAATTGSVATRTAPRSQVEGG"
     gene            1288..1521
                     /locus_tag="VFO47_07230"
     CDS             1288..1521
                     /locus_tag="VFO47_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788523.1"
                     /translation="MRMLIDHQHQPGAFDPGHCRNCAHEADLLDHYHRVQHEEQTVKQ
                     AHRRAWQARRHATLALWSSGIGILLAAIALLTG"
     gene            1541..2140
                     /locus_tag="VFO47_07235"
     CDS             1541..2140
                     /locus_tag="VFO47_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET8788524.1"
                     /translation="MRKIVAGLFMSLDGVVESPERWQFPYFNDEMGQALASSMAASDT
                     LLLGRRTYEEFAAVWPGRGSDDPVGAYMNTTPKLVASTTLDTVEWQNSTLLGGDLAEE
                     LGKLKQQPGRNVNVTGSATLVRSLLRHGLLDELGLLVCPIVVGRGHRLFEDVRQELPL
                     QLVDAETFSTGVLSLTYAPAATDGMEPPTDRQDSRATTT"
     gene            2264..>2793
                     /locus_tag="VFO47_07240"
     CDS             2264..>2793
                     /locus_tag="VFO47_07240"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET8788525.1"
                     /translation="MAAFLVEGATRPGYRPWRHAVSQLSLGPFGWVNTIAILLTALAL
                     LAFAAGLRSALPTGVGSTWGPRLIGVAGAGFLLAAAFPIDPGLGYPPGTSAQHSLAGL
                     VHGLVITVAFGCLSAACFVMARRFAGDPAWRGWARYSTVTGLVVATGYLATSVLTGLD
                     QAGVLPNAPGGVVQRA"
BASE COUNT          389 a         1055 c          922 g          427 t
ORIGIN      
        1 agccgcaggg gtacgtccgg gtctttgccg acgagggccc gcccatggcc gccctgctcc
       61 agcgcctcat ccgggcgcgt cagccaggcg gcgccgcgct gggttccggg gcagcgcgca
      121 accacctgaa ccgggtggtt cgtgcgttcc gggccccggc aggccacccg gagcgagccg
      181 agcctgcggc gagcggcctg gtcgaacccc tgaccaagcg ggagctggag gttctcggct
      241 tgatcgccgc cggcaagccg aaccaggaga tcgccgatga gctggtggtc accttggcga
      301 cggtgaagaa gcacaccagc cacatcttcg acaagttcgg cgcagcgagc cgcacccaag
      361 cggtggcccg ggcgcgcgag ctcggcctgc tcccctaggc acgctccgcg ccgctaccac
      421 cggtccaccc caagattctc cccgctgttc cacctttgca tgacgcccac gcccggggcc
      481 ctccctagcg tgcccacgtg acaggaaacg tcgacgcgtc catcgatcac caccaaccga
      541 aaggacaaga cggatgacac aagagctcac gacacccggt gccagcgcat ctggtgcagt
      601 ctccgagacg acccccaccg tcacccgagc gctactggtc tgcggcgtgg tcgccggccc
      661 cctgtacgtc gtcgtggccc tcctgcaggt cctgttccgc gacgggttcg acctcggccg
      721 gcacccgctc agcctgctga gcctgggcga gcttggctgg atccagatca ccaacttcat
      781 cgtcggcggt ctgctggcgg tcgggttcgc ggtcggcctg cggcgggtgc tgcatcccgg
      841 ccgcggcggg acctgggccc cactgctggt cggcggctac gggctggggc tgatcgccgg
      901 tggggtattc atcgccgacc cggccctcgg cttcccaccc gggaccccgg atggcatccc
      961 cgatcagttc agctggcatg gcctgctgca cgccttcgcc ccgccgctgg cgtcgctgtc
     1021 gctgatcgtg gcctgcttcg tcctcgcccg ccggttcgcc ggcctcgggc accggggctg
     1081 ggcaacctac agcgcggcca ccggcgtggc cgccctggcc ctcagcgcct ggcccaacca
     1141 ggccacggtc agcgtgcgcc tggcggtggc ggtcgtgatc ggctggacat gggtctcggt
     1201 gctggcaacc cggctgctgg tcgagcctgc cgccaccacg ggatcggtcg ctacccgcac
     1261 ggcaccgaga tcccaggtgg aggggggatg aggatgctga tcgaccacca gcaccagccg
     1321 ggggccttcg accccggcca ctgtcggaac tgcgcccacg aggccgacct gctggaccac
     1381 taccaccgcg tgcagcacga ggagcagacc gtcaagcaag cccatcggcg ggcatggcag
     1441 gcccgccgcc acgccacctt ggccctgtgg tcctccggca tcgggatcct gctggccgcc
     1501 atcgccctgc tcacgggctg acctgaccga ggagaagaac atgaggaaga tcgtcgcggg
     1561 actgttcatg tcgctggacg gcgtcgtcga gtcgcccgag cggtggcagt tcccctactt
     1621 caacgacgag atgggccagg cgctcgcatc gtccatggcc gcctcggaca ccctgctgct
     1681 gggtcgacgg acctatgagg agttcgccgc tgtctggcct ggccggggca gtgacgaccc
     1741 ggtcggcgcc tacatgaaca ccacccccaa gctggtcgcc tccaccaccc tggacaccgt
     1801 cgaatggcag aactcaaccc tgctcggcgg cgacctcgcc gaggagctcg gcaagctgaa
     1861 gcagcagccc ggcaggaacg tcaacgtcac cggcagcgcc accctggtcc ggtcgttgct
     1921 gcgccacggc ctgctcgacg agctggggct gctggtctgc ccgatcgtgg tcggccgcgg
     1981 ccaccggctg ttcgaggacg tgcgccagga gctgccgctc caactggtgg acgccgagac
     2041 gttcagcacc ggcgtcctgt cgctcaccta tgcgccggcg gccacggacg gcatggagcc
     2101 gcccaccgac cggcaggact caagagcgac aactacctga ctcaatcacg aatgattgga
     2161 gcatcagatg ctcggtacag ctgaccagac cggatccctg cgagaccgcc ctccacgtca
     2221 gcggttgctc ctgggtggtg ctgcggccgg tctcaccacc ttcgtggccg cgttcctggt
     2281 cgagggcgcg acccgccccg gctaccgtcc ctggcggcat gccgtcagcc agctcagcct
     2341 gggccccttc ggctgggtca acaccatcgc catcctgctg accgccctgg ccctgctggc
     2401 cttcgccgcc gggctccgct ccgccctgcc caccggggtc ggctcgacct gggggccacg
     2461 cctgatcggg gtggccgggg cgggcttcct tctggcggcc gccttcccca tcgacccggg
     2521 actcggctac ccgccaggca cgtccgccca gcacagcctg gctgggctcg tccacggtct
     2581 ggtcatcacc gtcgccttcg ggtgcctgag cgccgcctgc ttcgtcatgg cccgacgctt
     2641 cgccggcgat cccgcctggc gcggctgggc gcggtactcg accgtcaccg gcctggtggt
     2701 ggccaccggc tacctggcca cctcggtcct caccgggctg gaccaggccg gcgtgctccc
     2761 caacgcacca gggggtgtgg tccagcgggc cat
//
LOCUS       DASSWR010000432         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_186322,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000432 DASSWR010000000
VERSION     DASSWR010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186322"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1495
                     /gene="pknB"
                     /locus_tag="VFO47_07245"
     CDS             215..1495
                     /gene="pknB"
                     /locus_tag="VFO47_07245"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET8788526.1"
                     /translation="MATTPHVLLDRYEVGRLLGAGGMAEVFEGRDRLLARRVAIKVLQ
                     AQFVRDPSFLIRFKREAQAAASLSHPNIVGVYDTGTEDGTHFIVMEFVDGRTLKDVIR
                     AEGPLYPERAAEICADVCSALVAAHARGLIHRDIKPGNVMLTPEGKVKVMDFGIARAT
                     TSETITQTAAVVGTAQYISPEQAQGQTVDYRSDLYSVGCCLYEMLTGTVPFTGATPVA
                     IAYRHVREDPTPPRMLNGDVPAPLEAICLKAMAKLPDNRYQTAAELHDDLERFRNGQP
                     VHATPLMDSAATTQAIPRDGGADPTAMLGGPIAADRATRYAEPEEEERRTSVGWIVVS
                     LLALVAVGLAAFFITRALTGDDTPTATTVATPTTAAPTTQTTEAPTTQTTEAPTTQTT
                     AAPTTQTTEAPTTTDEPTTTEEEPTTSEPTTETT"
     gene            complement(1503..2132)
                     /locus_tag="VFO47_07250"
     CDS             complement(1503..2132)
                     /locus_tag="VFO47_07250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET8788527.1"
                     /translation="MSGTGAAPGDPGRGRRILVVDNYDSFVFNLVQYLGELGAETVVW
                     RNDAGTTEDVRALDPDGVVISPGPGHPRDARLSVDVVRDLGAELPVLGVCLGHQVIGH
                     VYGGRVGRAPELVHGKTSPIHHRGAGVLAGLPVPFAATRYHSLVVERQGLPEVLEVTG
                     ETAQGLVMALRHRSHPVEGVQFHPESILTDAGHQLLGNWLGGLRARAAA"
     gene            complement(2129..>2543)
                     /locus_tag="VFO47_07255"
     CDS             complement(2129..>2543)
                     /locus_tag="VFO47_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494905.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET8788528.1"
                     /translation="LLTGDMAVMDEEGYFAIVDRKKDLIIAGGYNVYPREVEEILYEH
                     HKVLEVAVAGVPDSYRGEIVKAFVVLRSGEQATTDEIREFAKARLAGYKVPRAVEFRD
                     ELPKTLIGKVLRRALVEEEQAKQAEEKADSGAETP"
BASE COUNT          375 a          989 c          865 g          314 t
ORIGIN      
        1 gggtgcgcgg ggctgtccac aacccccgga cccggcggga cggctcccgg ggggcggggt
       61 ggccggggtg gccgtggaga aggtcgtcgt gatcgctctg ctcctgctag cctggctgcc
      121 gcgcagcggg cgcctggatc cccccgaccg gtcgttccgg gcatccgcga acggccagcc
      181 ccaccaccgc cggccccggc gacgaggatg actgatggcg acgactccgc acgtgctgct
      241 ggaccgctac gaggttggcc gcctgctcgg cgccggcggc atggccgagg tgttcgaggg
      301 ccgcgaccgg ctgctcgccc gccgggtggc catcaaggtc ctccaggccc agttcgtccg
      361 cgaccccagc ttcctgatcc gcttcaagcg cgaggcccag gccgcggcca gcctcagcca
      421 ccccaacatc gtcggggtct acgacaccgg caccgaggac ggcacccact tcatcgtcat
      481 ggagttcgtg gacggccgca ccctcaagga cgtcatccgg gccgaggggc cgctgtaccc
      541 cgagcgcgcc gccgagatct gcgccgacgt ctgcagcgcc ctggtcgcgg cccacgcccg
      601 cggcctcatc caccgcgaca tcaagccggg caacgtgatg ctcacccccg agggcaaggt
      661 caaggtgatg gacttcggca tcgccagggc caccacctcg gagaccatca cccagacggc
      721 cgcggtcgtc ggcaccgccc agtacatctc ccccgagcag gcccagggcc agacggtcga
      781 ctaccgcagc gacctgtact cggtcggctg ctgcctgtac gagatgctga ccggcacggt
      841 gccgttcacc ggggcgaccc cggtggccat cgcctaccgc cacgtccgcg aggacccgac
      901 cccgccgcgg atgctgaacg gcgacgtgcc ggccccgctg gaggcgatct gcctgaaggc
      961 gatggccaag ctgcccgaca accgctacca gacggcggcc gagctccacg acgacctgga
     1021 gcggttccgc aacggccagc cggtgcacgc gaccccgctg atggactcgg ccgccaccac
     1081 ccaggcgatc ccccgcgacg gcggcgccga cccgacggcg atgctgggcg ggccgatcgc
     1141 ggccgaccgg gccacccgct acgccgagcc cgaggaggag gagcggcgga cctcggtcgg
     1201 ctggatcgtg gtcagcctgc tcgccctggt cgcggtcggg ctggccgcgt tcttcatcac
     1261 ccgggccctg accggggacg acaccccgac ggcgaccacc gtggcgacgc cgaccaccgc
     1321 ggccccgacc acccagacga ccgaggcccc gaccacccag accaccgagg ccccgaccac
     1381 ccagaccacc gcggccccga ccacccagac gaccgaggcc ccgaccacca cggacgagcc
     1441 gaccaccacc gaggaagagc cgacgaccag cgagccgacg accgagacga cctaacgtcc
     1501 ggtcaggcgg ccgcccgggc ccggagcccg ccgagccagt tgcccaggag ctggtgaccg
     1561 gcgtcggtga ggatcgactc ggggtggaac tggacgccct cgaccgggtg gctgcggtgg
     1621 cgcagggcca tgaccaggcc ctgggcggtc tccccggtca cctccagcac ctcgggcagg
     1681 ccctgtcgct ccaccaccag cgagtggtag cgggtggccg cgaacggcac cggcagcccg
     1741 gccagcactc ccgcaccccg gtggtggatt ggcgaggtct tgccgtggac cagctcgggg
     1801 gcgcggccca cccggccgcc gtagacgtgg ccgatgacct ggtggccgag gcagacgccg
     1861 aggacgggga gctcggcccc gaggtcgcgg acgacgtcga ccgagagccg ggcgtcgcgg
     1921 ggatgccccg gccccggcga gatcaccacc ccgtccgggt cgagggcgcg aacgtcctcc
     1981 gtggtcccgg cgtcgttgcg ccacacgacc gtctcggccc ccagctcccc caggtactgg
     2041 accaggttga agacgaacga gtcgtagttg tcgacgacca ggatccgccg cccccggccc
     2101 gggtcgcccg gcgcagcccc ggttccgctc atggcgtctc ggctccgctg tccgccttct
     2161 cctcggcctg cttggcctgc tcctcctcga ccagggcgcg gcgcaggacc ttgccgatca
     2221 gcgtcttggg cagctcgtcg cggaactcga cggcacgcgg gaccttgtag ccggccaggc
     2281 gggccttggc gaactcgcgg atctcgtcgg tggtcgcctg ctcacccgac cgcaggacca
     2341 cgaacgcctt gacgatctcg ccccggtagc tgtcgggcac cccggcgacg gccacctcga
     2401 ggaccttgtg atgctcgtag aggatctcct cgacctcgcg cgggtagacg ttgtagccgc
     2461 cggcgatgat caggtccttc ttgcggtcga cgatcgcgaa gtagccctcc tcgtccatca
     2521 ccgccatgtc cccggtgagc agc
//
LOCUS       DASSWR010000433         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_270960,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000433 DASSWR010000000
VERSION     DASSWR010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270960"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1265)
                     /locus_tag="VFO47_07260"
     CDS             complement(201..1265)
                     /locus_tag="VFO47_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HET8788529.1"
                     /translation="MAERNPAVGVVDTLLRGVGQVMFQNNPLTGLLFLVGILVNSFKF
                     CGAGLLALAASTLAAHLLGADRALIRAGLFGFNGILVGIALAFFFEFDLLLAAYIVLG
                     AVVSTIVMMALINLLGPWDMPALTAPFVLTAWLLLFAVYQFDFLQPTELIAPAAPDPA
                     ATVQTELRELATGTGGLTAANLAHGFFRGVGEVMFEDNLLTGVIFLVAIAVNSRISAA
                     FAALGAAIGLLTALALGGDGFTAYHGLYGFNAVLCAIAIGGLFFVLTWRSAIYALLAA
                     AFSVIAFAAIAVLLAPIGMPALTAPFVLTTWLFLWPKAGFRALTPVALADITTAENIR
                     AAYRGRAAGPSATAEPGEAT"
     gene            complement(1414..>2022)
                     /locus_tag="VFO47_07265"
     CDS             complement(1414..>2022)
                     /locus_tag="VFO47_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET8788530.1"
                     /translation="KVAREGARTVPARENGGNHDIKNFTRGSRVFYPVHVPGAKLSGG
                     DLHFSQGDGEVTFCGAIEMGGFIDFGVDLIKGGMEKYGVSTNPVFMPGNVEPRYSEFV
                     TFIGVSVDHDTNTNYYMNATLAYKRACLNAIEYLKTFGYSGPQAYLLLGSAPIEGRIS
                     GIVDIPNACASLYLPTAIFDFDIRPTADGPRKADRGQCAMTS"
BASE COUNT          338 a          660 c          751 g          273 t
ORIGIN      
        1 cgtggccgac tgcgacgcga caacctcccc gcccaccacg gtcgccgagc cgcccacgtc
       61 caccacgccc accacgatcg acgacggcgg caacggcaac gggcacggca acggcaacgg
      121 caacgggtac ggcaaccgca acacgacgcc catcgagctc ggctaggcgc gacccccgaa
      181 tctcgaggat cgccggcgcc tcacgtcgct tcgccgggct ccgcggtggc gctggggcct
      241 gccgctcggc cccgataggc tgcccggatg ttctcggcgg tggtgatgtc ggccagtgct
      301 accggggtga gcgcccggaa gccggccttg ggccacagga acagccaggt ggtgagcacg
      361 aagggggccg tcagggctgg catgccgatg ggggcgagca gcaccgcgat ggcggcgaat
      421 gcgatcacgc tgaacgccgc ggccagcagc gcgtagatgg ccgagcgcca ggtgagcacg
      481 aagaacagcc cgccgatggc gatggcgcac agcacggcgt tgaagccgta cagcccgtgg
      541 taggcggtga agccgtcgcc gcccagggcc agggcggtca gcaggccgat ggccgccccg
      601 agggcggcga aggcggccga gatccgcgag ttgacggcga tggcgaccag gaagatcacc
      661 ccggtcagca ggttgtcctc gaacatcacc tcgcccacgc cgcggaagaa gccgtgggcc
      721 aggttggccg cggtcaggcc cccggtgccg gtggccagct cccgcagctc ggtctggacc
      781 gtggccgccg ggtcaggggc cgcaggggcg atcagctcgg tcggttggag gaagtcgaac
      841 tggtagacgg cgaacagcag cagccaggcg gtgagcacga acggggcggt cagcgccggc
      901 atgtcccagg ggccgagcag gttgatcagg gccatcatca cgatcgtgga gacgaccgcc
      961 ccgaggacga tgtaggcggc cagcaggagg tcgaactcga agaagaaggc cagggcgatc
     1021 ccgaccagga tgccgttgaa cccgaagagg ccggcccgga tcagcgcccg gtcggccccc
     1081 agcaggtgag cggccagggt gctggcggcc agggcgagca ggccggcgcc gcagaacttg
     1141 aacgagttga ccaggatgcc gacgaggaac agcaggccgg tgagggggtt gttctggaac
     1201 atgacctggc cgacaccgcg caggagggtg tccaccaccc cgactgccgg gttgcgctcg
     1261 gccatcgtgg cccagctttc tgccgcgcgc tgtggcgcgc cccgatctgc catggtgttc
     1321 cgctctcctc ccgaatccga gccagcgaag ctgccagcag caggacggcc ggacgcccgg
     1381 cgtggtgccg ggcgtccggc gtgctgcctg ccgctaggag gtcatggcgc actgcccccg
     1441 gtcggccttc ctcgggccgt cggcggtggg ccggatgtcg aagtcgaaga tcgcggtcgg
     1501 caggtacagg gaggcgcagg cgttggggat gtcgacgatg ccgctgatcc ggccctcgat
     1561 cggggccgag ccgagcagca ggtaggcctg cggaccggag tagccgaacg tcttgaggta
     1621 ctcgatggcg ttcaggcagg cccgcttgta ggccagcgtg gcgttcatgt agtagttggt
     1681 gttggtgtcg tggtcgaccg acacgccgat gaaggtgacg aactcgctgt agcggggctc
     1741 gacgttgccg ggcatgaaca ccgggttggt gctgactccg tacttctcca tgccaccctt
     1801 gatcaggtcg acgccgaagt cgatgaagcc gcccatctcg atggcgccgc agaaggtgac
     1861 ctcgccgtcg ccctggctga aatggaggtc gccacccgac agcttggccc cggggacgtg
     1921 gaccgggtag aagacccgcg agcccctggt gaagttcttg atgtcgtggt tgccgccgtt
     1981 ctcgcgggcc gggacggtcc tggccccctc gcgggccacc tt
//
LOCUS       DASSWR010000434         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_44771,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000434 DASSWR010000000
VERSION     DASSWR010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44771"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFO47_07270"
     CDS             complement(<1..550)
                     /locus_tag="VFO47_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HET8788531.1"
                     /translation="MQVSVCESLQVQAIDGAGGKMTVLDVRSIVLPAPRRGSVNPLPP
                     LRLLGDLHDDIDCTGLDAEMTRNIRYGNVGSVLPYLLQDDYSRELVPQETRVATLENG
                     HLLAMVLLDLGGRLWSLQDKVGGRELLYNNDRIQLGNLGLRNAWFPGGVEWNLGTTGH
                     TALTCSPVHAASLGFPDGTPGLR"
     gene            549..1274
                     /locus_tag="VFO47_07275"
     CDS             549..1274
                     /locus_tag="VFO47_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8788532.1"
                     /translation="MVGHRDDERLETGDGVRHVVWDWNGTLFDDHVAVVAAINDALAL
                     LSLRPIDSDTFRTHFTRPVQRFYEQVAGRPIEPGEWHILDEAYHDSYAGRLERLRLAP
                     DAWAALAAAETAGLTQSLLSMWRHQDLVSLVERLEIGRFFRRVDGLRAPGGGGKAEHL
                     VAHLAALEVEPSAALLVGDALDDLAAARAVGARCVLYDGGAHHRHALEAAGVPVVDTL
                     TAAVATITASRRRPGSARHRPAR"
     gene            complement(1222..2304)
                     /locus_tag="VFO47_07280"
     CDS             complement(1222..2304)
                     /locus_tag="VFO47_07280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8788533.1"
                     /translation="MRVIHVAPSVFGPDGLYGGGERYPLELARALADQVDCELVTFGP
                     RPATWRADGGLRVRVLRPVTWLRGHPAQPVAPLLPAALTRAQVVHAHHFRSVPTRMSA
                     VTARVLGAGTAVTDHGLLGRTWGGLVHRLFDRFLAVSRCSAELLGAPPARTQVIYGGA
                     DTRRFTPVPGADRDGVLFVGRLTPHKGIDRLIRALPAGAGLRIAGSAGHDPRPPERDY
                     PALLHRLAQDHDVTFLGPVPDADLPGLYRRAAVLALPSVDRTCYGRHVPVSELLGLVA
                     LEAMASGTPVVASRIGGLAEVVVDGETGFLVPPGDTEALAEGLARLLSDRRLAARLGA
                     NARDLVVHRFTWRACAERCLAAYETL"
     gene            2294..>2932
                     /locus_tag="VFO47_07285"
     CDS             2294..>2932
                     /locus_tag="VFO47_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8788534.1"
                     /translation="MTRILTVSLVTLGDPGRLTGGYRYHRRMAELAPACGAAVRFVSF
                     PDRPFPLPASSGPAVLRRARADVLVLDSIAAAFLGPWLPRRPRTPLVGSLHQPPGGID
                     HGPLRRAVQAPVDRLAYRRARLLLVASQALGDELAGQGYPPERIRVVPPGHDLPAAPA
                     GPPVDLRQGRQASLLCVANWVERKGLLDLLEAVARLPDGLATLHLAGDDRADP"
BASE COUNT          394 a         1066 c         1086 g          386 t
ORIGIN      
        1 tgcgaaggcc tggggtgcca tcggggaacc cgagggaagc cgcgtggacg ggcgaacagg
       61 tcaatgccgt gtgtcctgtg gttcccaggt tccactcgac tccgccgggg aaccaggcat
      121 ttcgcaaccc gagattgccg agctgtatcc ggtcgttgtt gtagagaagc tctcgaccgc
      181 cgaccttgtc ctgcagcgac cagagccggc caccaaggtc gagaaggacc atggcgagca
      241 gatggccgtt ctcaagggtc gccacgcgag tctcctgtgg cacgagctcg cgggagtagt
      301 catcctgcag caggtacggc agcacgctgc ccacgtttcc gtaacggatg ttgcgcgtca
      361 tctccgcgtc cagcccggtg caatcgatgt cgtcgtgcag atccccgaga aggcgcaacg
      421 gaggaagagg attcacgctc ccccgccgtg gcgcaggcaa gacgatcgag cggacatcga
      481 ggaccgtcat tttcccccca gcaccgtcaa tcgcctgaac ctgcaggctc tcacatacac
      541 tgacctgcat ggtggggcac cgggatgatg agcgcctcga gaccggggac ggcgtccgtc
      601 acgtggtgtg ggactggaac ggcacgttgt tcgacgacca cgtcgcggtg gtggcggcga
      661 tcaacgacgc gctggcgttg ctgagcctcc ggccgatcga ctccgacacc ttccgcacgc
      721 acttcacccg gccggtccag cggttctatg agcaggtggc cggccgtccc atcgagccgg
      781 gcgaatggca catcctcgac gaggcctacc acgacagcta tgccggccgg ctggaacgcc
      841 tccggctggc ccccgacgcc tgggcggcgc tggcggccgc cgagacggcc gggctcaccc
      901 agtcgttgct gtcgatgtgg cgccaccagg acctggtgtc gctggtcgag cggctggaga
      961 tcggccgttt cttccgccgg gtggatgggc tgcgcgcgcc tggtgggggc ggcaaggccg
     1021 agcacctggt ggcccacctg gctgcgctcg aggtcgagcc ctcggcggcg ctgctggtgg
     1081 gagacgcgct cgacgacctg gcggcggcgc gggcggtcgg cgcccgctgt gtgctgtacg
     1141 acggaggcgc acatcaccgg cacgcgctcg aggcggccgg cgtcccggtg gtcgacacgc
     1201 tcaccgccgc cgtggccacg atcacagcgt ctcgtaggcg gccaggcagc gctcggcaca
     1261 ggcccgccag gtgaaccggt gcacgaccag gtcgcgggcg ttggcgccca gccgggcggc
     1321 caggcggcgg tccgacagca ggcgcgcgag gccctcggcg agcgcctcgg tgtcgcccgg
     1381 cgggaccagg aacccggtct cgccgtccac gacgacctcg gccagccccc cgatccggct
     1441 ggcgaccacc ggggtcccgc tggccatggc ctccagcgcg accaggccga gcagctcgga
     1501 gacggggacg tgccggccgt agcaggtccg gtcgacggac gggagggcca ggaccgcggc
     1561 ccgccggtac aggccgggca ggtcggcatc cggcaccgga ccgaggaagg tcacgtcgtg
     1621 gtcctgggcg agccggtgca gcagcgcggg atagtcccgc tcgggtgggc gggggtcgtg
     1681 gccggcgctg cccgcgatcc ggagcccggc gccggcgggc agggcgcgga tcagccggtc
     1741 gatccccttg tgcggggtca gccgcccgac gaacaggaca ccgtcccggt cggcgccggg
     1801 cacgggggtg aaccgccgcg tgtcggctcc gccgtagatc acctgcgtcc tcgccggggg
     1861 ggctccgagc agctcggcgg agcatcggga gacggccagg aagcggtcga acagccggtg
     1921 cacgagcccg ccccaggtcc tgccgagcag cccgtggtcg gtgacggccg tgcccgcccc
     1981 gaggacgcgg gccgtgaccg ccgacatccg ggtgggtacg ctcctgaagt gatgggcgtg
     2041 gacgacctgg gcgcgggtga gcgccgcggg gagcagcggg gccaccggct gggcagggtg
     2101 gccgcgcagc caggtcaccg gccggaggac ccggacgcgc aggccgccgt cggctcgcca
     2161 cgtggcgggc cgcgggccga aggtgaccag ctcgcagtcc acctggtcgg ccagggcccg
     2221 ggccagctcc aggggatagc gctcgccccc gccgtacagc ccgtccggcc cgaacacgct
     2281 cggagccacg tggatgaccc gcatcctcac cgtctccctg gtcacgctgg gtgaccccgg
     2341 ccgcctgacc ggcggctacc gttatcatcg ccgcatggcg gagctggcgc cggcctgcgg
     2401 cgccgccgtc cgtttcgtct ccttccccga ccgccccttc cccctgccgg catcctccgg
     2461 cccggccgtg ctgcgccggg ccagggcgga cgtcctggtg ctggacagca tcgccgccgc
     2521 cttcctcggg ccctggctac cccggcggcc caggacgccg ctggtgggca gcctgcacca
     2581 accgcctgga gggatcgatc acgggccgct gcggcgggcc gtccaggccc cggtggaccg
     2641 gctggcctac cgccgcgccc gcctgctcct ggtggccagc caggccctcg gtgacgagct
     2701 ggccggccag gggtatccgc ccgaacggat ccgggtggtg ccgccgggcc acgacctgcc
     2761 cgccgctccg gccggcccgc cggtcgacct ccgccagggt cgccaggcga gcctgctgtg
     2821 tgtggccaac tgggtggaac gcaagggcct cctcgacctc ctcgaggccg tggcccggct
     2881 gccggacggg ctggccaccc tgcacctggc cggggacgac cgcgccgatc cc
//
LOCUS       DASSWR010000435         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_327841,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000435 DASSWR010000000
VERSION     DASSWR010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327841"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFO47_07290"
     CDS             <1..1108
                     /locus_tag="VFO47_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8788535.1"
                     /translation="ACRYRQQGRDRLALLDPESGGRKDLPVPYTSLKPYLRAAGDRLA
                     FIGASPTASSAVAILHVPTGDLDVLAGAEVSLDPAWVSVSKPIQFPARDGQTAHGLYY
                     PPTNPEVTGPADARPPLLVQAHPGPTADAKARLDLRTQFFTSRGFAVVDVNYAGSTGY
                     GRGYRERLTGQWGILDVTDCLDAVRSLVEAGEVDGRRLVISGESAGGFTALCALASED
                     RLAVGTSRFGIADLETFRQQAPTFQAHELDRLVGPYPEEAATYRARSPLHAVDRIARP
                     VLLVHGLEDTVVPPSQAQVMAEALARRGVQDLLVAFPGEGHGFRRPESIRRALEVELS
                     FYLETLGLTHGKSDAPLATDDAIAVSSSDQRQGR"
     gene            complement(1164..>2280)
                     /locus_tag="VFO47_07295"
     CDS             complement(1164..>2280)
                     /locus_tag="VFO47_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8788536.1"
                     /translation="LARAKRAKIALAAPTGRAAKRLSELAGLEAATLHRLLRLRPGGD
                     AAFDRDHPLDADLVVVDEASMLDVLLANKLVKAIPPGAHLLLVGDVDQLPSVGAGEVL
                     RDLLAAERLPRVRLSKVFRQAQHSGVVTNAHRINAGQHPVTYGLADFFLFPEEESEQV
                     ADLVVEIVANRLPRRFGLDATRDVQVLCPMHRGPAGAGVLNERLQAALTPAREGLAER
                     RFGGRIYRVGDKVMQLRNNYDKGTAGVFNGSVGVVTALSLEDSELRVLLDEDEEVGYG
                     FDELDELTHAYAVSIHRSQGSEYPCVVIPLTTSAWLMLRRNLLYTAVTRAKQMVVLVG
                     SRRALAKAVRTPGAGRRYTALTDRLQPGSVGATAAAS"
BASE COUNT          332 a          807 c          786 g          355 t
ORIGIN      
        1 cgcctgtcgc taccggcagc agggccgcga ccggctcgcc ctgctggacc cagagagcgg
       61 cgggcgcaag gacctgccgg ttccctacac ctcgctcaag ccctaccttc gagcggcggg
      121 ggaccgtctg gcgttcatcg gcgccagccc cacggccagc tcggccgtgg cgatcctgca
      181 tgtgccaact ggtgacctcg acgtccttgc cggggccgag gtttccctgg acccggcctg
      241 ggtgtcggtg tcaaagccga tccagttccc ggcccgggac ggccagacgg cccacgggct
      301 ctattaccca cccaccaacc cagaagtcac cgggccggcg gatgcccgac ctccgctgct
      361 ggtgcaggcc catcctgggc cgaccgccga cgccaaggcc cgccttgacc tgcggaccca
      421 gttcttcacc agccgggggt tcgccgtggt cgatgtcaac tacgccggct cgaccggcta
      481 cggccgcggc taccgcgagc gcctgaccgg ccagtggggc atcctggacg tcaccgattg
      541 cctggacgca gttcgctcct tggttgaggc gggggaggtg gatgggcggc ggctggtgat
      601 cagcggcgaa agtgccggtg gctttacggc cctgtgcgcg ctcgcctccg aggatcgctt
      661 ggccgtcggc accagccggt tcggcatcgc cgatctggag accttccgcc agcaagcccc
      721 cacgttccag gcgcacgagc tggatcggct ggttggcccc tatccggagg aggcggccac
      781 ctaccgggcc cgctccccgc tgcatgccgt ggaccggatt gcccgtccgg tgctgctggt
      841 gcatgggctg gaggacaccg tggtgccacc gagccaagcc caggtcatgg cggaggcgct
      901 tgcgcgcagg ggcgttcagg acctcttggt tgcctttccc ggtgaggggc atgggtttcg
      961 ccgccccgag agcatccgcc gtgcgctcga ggttgagctg tccttctacc ttgaaacgct
     1021 aggtctgacg cacggcaagt ccgatgcgcc tctcgccacc gatgacgcga tcgcagtcag
     1081 ctcgtccgat cagcggcagg gcaggtaggt cgccgatcag gcggatcaca ggcgaatggc
     1141 tggaagcgct ggtaggcagc ggctcaactg gcggcggcgg tggccccgac ggatcctggc
     1201 tggagccggt cggtcagggc ggtgtagcgg cggccggcgc ccggggtccg gaccgccttg
     1261 gccagggcgc gccggctgcc gaccagcacc accatctgtt tggcccgagt gacggcggta
     1321 tagagcaggt tgcgccgcag catcagccag gcgctggttg tcagggggat gaccacacac
     1381 gggtattcgc tgccctggga acggtggatg ctgaccgcat aggcatgggt caactcatcc
     1441 agctcgtcga acccatagcc aacctcctca tcctcatcca gcagcacccg cagctcgctg
     1501 tcctccagcg agagcgcggt gaccacccca accgagccgt tgaacacccc agcggtgccc
     1561 ttgtcgtagt tgttccggag ctgcatcacc ttgtcgccga cccggtagat gcgcccgccg
     1621 aagcgtcgct cagcaaggcc ctctcgggct ggcgtcagcg cggcctgcag ccgctcgttc
     1681 agcacgcctg cgccggccgg gccccggtgc atcgggcaca gcacctgcac gtcgcgggtc
     1741 gcgtcgagtc cgaagcggcg gggcagccgg ttggccacaa tctccaccac caggtcggcg
     1801 acctgttcgg actcctcctc ggggaacaag aagaagtcgg caaggccata ggtgaccggg
     1861 tgctggccgg cgttgatccg gtgggcgttg gtcaccaccc cgctgtgttg ggcctgccga
     1921 aacaccttgc ttagccgcac ccggggcagc cgctcggcgg ccagcaggtc ccgcagcacc
     1981 tcccccgctc cgacgctggg cagctggtcc acgtcgccga ccagcagcag gtgcgccccg
     2041 ggcgggatgg ccttgaccag cttgttggcc aatagcacat ccaacatgga ggcttcgtcg
     2101 accaccacca ggtcggcgtc caggggatgg tcgcggtcga aggcggcgtc cccacccggg
     2161 cgcagccgca gcagccgatg cagggtggcc gcctccagac cggccagctc tgagaggcgc
     2221 ttggcggccc gcccggtcgg ggcggccagg gcgatcttgg cccgcttggc gcgggccaag
//
LOCUS       DASSWR010000436         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_525479,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000436 DASSWR010000000
VERSION     DASSWR010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525479"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1441
                     /locus_tag="VFO47_07300"
     CDS             104..1441
                     /locus_tag="VFO47_07300"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET8788537.1"
                     /translation="MRAWHAELAWLGPERGVAERVLVEVEGERIAAVTEGVDPPGGAT
                     RLGGVTIPGMANGHSHAFHRALRGRTHRGRGDFWSWRELMYRVAGALDPDRYLELATA
                     AYAEMALAGITAVGEFHYLHHDPDGRPYADPNLMGESLAEAAVRAGVRLTLIDTCYLR
                     AGLDGQPLAGPQVRFGDGDAEAWAERAGALRDRPGLRVAAGIHSVRAVDPAAMTTVAA
                     WAAEHQAPLHLHLSEQRAENEACLAATGRTPAALAESAGVLGPRTTAVHATHLTTEDI
                     GLLGATRTTVCICPTTERDLADGVGPARALADAGSPLCLGSDSHAVVDLFEEARAVEL
                     DERLITGRRAHHQPPDLLAAATGSGMDALGWDAGRLAPGRLADLVTVGLGSVRLAGAH
                     PADAVDQLVFAATAADVTSVVVSGRQVVQDSQHLLVGEVAAALDRAIAALDDR"
     gene            complement(1485..>2220)
                     /locus_tag="VFO47_07305"
     CDS             complement(1485..>2220)
                     /locus_tag="VFO47_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET8788538.1"
                     /translation="RIEESLLRRALGLASIRIQSAGRTGTADQSAGRLAIPILQRMQV
                     NRVLNELVAGAAPVPRLLAPPPAARRRSVTRSVVGAGVLVAAVALPVWVLTSAGVLEV
                     PPALALAALPVLGVAVAAGLAAYRSLGHATGEGFLYARAGVAIRVLTVVPVAKAQSGS
                     VRSSPFQRRAGLATLHVDIAGGGPTPQVHDESEPRAEDLLQVILGRRVVPGSSAAPGP
                     SGGGSVGDPGQPGTRPAGGGSPGGGA"
BASE COUNT          267 a          850 c          864 g          239 t
ORIGIN      
        1 ggcccccggt gggggagcgt agccgacgcc ggcggtggct gccggcgact tcccacagcc
       61 ccggccttgt tcccggcctc ggccgacccc cggcaggccg ggcatgaggg cctggcacgc
      121 cgagctggcc tggttggggc cggaacgggg ggtggccgag cgggtcctgg tcgaggtgga
      181 gggggagcgg atcgcggcgg tgaccgaggg ggtcgacccg ccgggcgggg cgacccggct
      241 gggcggggtg accattcctg ggatggccaa cgggcactcg catgcgttcc accgggcgct
      301 acgagggcgc acccatcgcg gccgggggga cttctggagc tggcgggagc tgatgtaccg
      361 ggtcgccggg gcgctcgacc cggaccgcta cctggagctg gccacggccg cctacgccga
      421 gatggcgctg gccgggatca ccgccgtggg ggagttccac tacctgcacc acgacccgga
      481 cggccgcccc tacgccgacc ccaacctgat gggggagtcg ctggccgagg cggccgtccg
      541 ggccggggtc cggctcaccc tgatcgacac ctgctacctg cgggccgggc tcgacggcca
      601 gccgctggcc ggtccgcagg tccgcttcgg ggacggcgac gccgaggcct gggccgagcg
      661 ggccggagcc ctgcgcgacc ggccgggact gcgggtggcg gccgggatcc acagcgtccg
      721 ggcggtcgac ccggcggcca tgaccacggt ggccgcctgg gccgccgagc accaggcgcc
      781 gctgcacctc cacctgtctg agcagcgggc cgagaacgag gcctgcctgg ccgccaccgg
      841 ccgcaccccg gccgccctgg ccgagtcggc cggggtgctc ggcccccgga ccaccgccgt
      901 ccacgccacc cacctgacca ccgaggacat cggcctactg ggcgccaccc ggaccaccgt
      961 ctgcatctgc cccaccaccg agcgcgacct ggccgacggg gtcgggccgg ccagggcgct
     1021 ggccgacgcc ggcagcccgc tctgcctcgg ctccgacagc catgccgtgg tcgacctgtt
     1081 cgaggaggcc agggcggtcg agctggacga gcgcctgatc accggacgcc gcgcccatca
     1141 tcagcccccc gacctgctgg ccgcggccac cgggtcgggc atggacgccc tcggctggga
     1201 cgccggccgc ctcgccccgg gtcgcctggc cgacctggtc acggtcgggc ttggctcggt
     1261 ccgcctggcc ggtgcccacc ccgccgacgc cgtcgaccag ctggtgttcg cggccaccgc
     1321 cgccgacgtg acctcggtcg tggtgagcgg ccgccaggtc gtccaggaca gccagcacct
     1381 gctcgtcggc gaggtcgccg ccgcgctcga ccgcgccatc gccgccctcg acgaccgctg
     1441 acccggggtg tggacaaccg aacgcatgcc cgctcctggc tgggctacgc tcccccaccg
     1501 ggggacccgc caccggcggg tcgggttcca ggctggccag ggtcaccaac ggacccgcca
     1561 ccggagggtc cgggtgcggc ggaggatccg ggaacgaccc ggcggcccag aatcacctgg
     1621 agcaggtcct cggcgcgggg ttcggactcg tcgtggacct ggggggtggg gccgccgccg
     1681 gcgatgtcga cgtggagggt ggccaggccg gcgcggcgtt ggaacgggga ggagcggacg
     1741 ctgccgctct gggccttggc gaccgggacg acggtgagga cccggatcgc caccccggcc
     1801 ctggcgtaca ggaatccctc gccggtggcg tggccgaggc tgcggtaggc ggccaggccg
     1861 gcggccacgg ccacccccag caccggcagc gcggccaggg cgagcgccgg cgggacctcc
     1921 agcaccccgg cggaggtcag gacccacacg ggcagggcga cggcggccac gagcacgccc
     1981 gccccgacga cgctgcgggt caccgaccgc cgcctggccg ccggcggcgg ggcgagcagc
     2041 cgcggcaccg gggccgcccc cgccaccagc tcgttcagga cccggttgac ctgcatccgc
     2101 tggaggatgg ggatggccag ccgcccggcc gactggtcgg cggtgccggt ccggccggcg
     2161 ctctggatgc ggatcgaggc caggccgagg gcccgccgca gcagcgactc ctcgattcgg
//
LOCUS       DASSWR010000437         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1118337, whole genome shotgun sequence.
ACCESSION   DASSWR010000437 DASSWR010000000
VERSION     DASSWR010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118337"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1135
                     /locus_tag="VFO47_07310"
     CDS             251..1135
                     /locus_tag="VFO47_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788539.1"
                     /translation="MLELRGLSRRYGDVVALEDLSFTVREGQMFGFVGPNGAGKTTTM
                     RIVLGVLQADRGEVRWRGRPVDAETRRRFGYMPEERGLYPKMRVRDQLEYFARLHGLS
                     AGDAANAAGYWIERLGVAERAGDRVEQLSLGNQQRVQLAAALVHNPEVLVLDEPFSGL
                     DPVGVDVLAEVLADRANDGIPVIFSSHQLELVERLCEAVAIINHGRLVAAGPVEELRA
                     GGGERRWQVEVADTAGDWVAGLEGVTVLEKHNGRVVLAVDGDEQRVLDAARGAGRVTL
                     FGAVQPTLAQLFRQAVQE"
     gene            1132..2358
                     /locus_tag="VFO47_07315"
     CDS             1132..2358
                     /locus_tag="VFO47_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788540.1"
                     /translation="MRTPRTSTYDGIKLTARRELVERTKRDRSFLISTLITLAILVAI
                     IFVPKLLGSDDPEEFDVGLVGAASQPLGQALTAQGEAAGVRIRTRQPAAAAEAEAAVR
                     DDSLDLAVIDGRELVAKADVDEQLNLLVQGASGAVRAQQTLQAAGVEPGEIQAALAPP
                     PLPVRSLEPVDEDARSKRTIATVAVFLLYGQLIGYCFAVSMGVVEEKSTRVVEVLLAT
                     VRPVQLLAGKIIGIGLVGLLQLAVIGAVGLAVAVASNAITLPPDAAGTIGSVLLWFLL
                     GYAFYSSMFAVAGAIVSRQEELQNTAAPLNLLMIASFFVAFSSSVGGGDSTLAKVSSF
                     LPPVAPLVMPVRIAGGDAALWEIGLSLVIMLVSTVAVVVLAARLYEGAILRTGARVKL
                     RDAWRGARRQPAAGRA"
     gene            complement(2506..3186)
                     /locus_tag="VFO47_07320"
     CDS             complement(2506..3186)
                     /locus_tag="VFO47_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788541.1"
                     /translation="MTASEAQPIGVLVVDDDPLVRAGLMMMLGGAPDLRVVGEAGDGA
                     QVPAMVDRHRPDVVLMDIRMPAMDGLAATEALRARRNAPEVIILTTFDADEHVLRALR
                     AGAAGFVLKDTPPAEIVDAVRRVAQGLPVLSPAVTRRLISRVAGADHDRRTRARERLA
                     LLNDREREVAVAVGQGRSNAEIGAVLYLSVPTVKTHVSSILTKLDLNNRVQIALLAHD
                     AGLLDDND"
     gene            complement(3183..>3879)
                     /locus_tag="VFO47_07325"
     CDS             complement(3183..>3879)
                     /locus_tag="VFO47_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET8788542.1"
                     /translation="QLVESLRDRAVRAETEAQLRAEQAQQRARDEIAREMHDVLGHRL
                     SLLSVHAGALEFRPDAPAEEVARAATVIRENAHQALQDLREVIGVLRAPVGELPQPTL
                     ADVAQLVAESGRAGMRVALRDETSGAVPDLAARTAYRVVQEALTNARKHAPGAEVLVH
                     LAGSPGNGLTVEVRNQPVPGPASGPGPAKDPGAGQGLVGLAERVALANGRLEHGPTGG
                     GGWRLAAWLPWPP"
BASE COUNT          463 a         1364 c         1529 g          523 t
ORIGIN      
        1 gagccccggc cgcgcaggct gcggcgtgct gacggagcgt cggagggcta ctcccctcga
       61 cctgacatcc cgcagtgtac gtcggtcgga ttcaaggtcg tgtaaggacc gcccacccct
      121 tcctggacgg gtccggctcc acccggggga ggacggcaag gtcgtcccgg gggccgacgg
      181 cggcggcggg ggtcgctggt caggtgcgcg cgagggggat gggcagggga gagaagccgg
      241 agggaggaag gtgctcgagc tgcgggggct gtcccggcgc tacggcgacg tcgtcgccct
      301 ggaggacctg tcgttcacgg tccgcgaggg gcagatgttc gggttcgtcg ggccgaacgg
      361 cgccggcaag accaccacca tgcggatcgt gctcggggtg ctgcaggccg accggggcga
      421 ggtccgctgg cggggccggc cggtcgacgc cgagacccgc cgccgcttcg gctacatgcc
      481 ggaggagcgc ggcctgtacc ccaagatgcg ggtacgcgac cagctggagt acttcgcgcg
      541 cctgcacggc ctgtcggccg gggacgcggc gaacgcggcc ggctactgga tcgagcggct
      601 gggggtggcc gagcgggccg gcgaccgggt ggagcagctg tcgctcggca accagcagcg
      661 ggtgcagctg gccgccgccc tggtccacaa ccccgaggtc ctggtcctgg acgagccgtt
      721 ctcggggctg gacccggtcg gggtcgacgt gctggccgag gtgctggccg accgggcgaa
      781 cgacggcatc ccggtgatct tctccagcca ccagctggag ctggtcgagc ggctgtgcga
      841 ggcggtcgcg atcatcaacc acggccgcct ggtcgccgcc gggccggtcg aggagctgcg
      901 ggccggcggc ggcgagcgcc gctggcaggt ggaggtggcc gacacggccg gcgattgggt
      961 ggccggcctg gagggggtga cggtgctgga gaaacacaac gggcgggtgg tgctggccgt
     1021 cgacggcgac gagcagcggg tgctcgacgc ggccaggggc gccggtcgcg tgaccctgtt
     1081 cggcgccgtc cagccgaccc tggcccagct cttccggcag gcggtacagg aatgaggacg
     1141 ccacgcacga gcacctacga cggcatcaag ctgacggcca ggagagagct ggtcgagcgg
     1201 accaagcgcg accgctcgtt cctcatctcc accctgatca ccctggccat cctggtcgcg
     1261 atcatcttcg tgcccaagct gctcgggtcc gacgacccgg aagagttcga cgtcgggctg
     1321 gtcggcgcgg cctcccagcc gctcggccag gcgctcaccg cccagggtga ggcggccgga
     1381 gtccgcatcc ggacgcggca gccggccgcc gcggccgagg ccgaggcggc cgtgcgcgac
     1441 gacagcctcg acctggccgt gatcgacggg cgcgagctgg tggccaaggc cgacgtcgac
     1501 gagcagctca acctgctcgt gcagggagcc tcgggggcgg tccgggccca gcagaccctt
     1561 caggcggccg gcgtcgagcc gggcgagatc caggctgccc tggcccctcc cccgctgccc
     1621 gtgcgcagcc tggagccggt cgacgaggac gcccgctcca agcggacgat cgccaccgtg
     1681 gccgtgttcc tcctctacgg gcagctcatc ggctactgct tcgccgtctc gatgggcgtg
     1741 gtcgaggaga agtcgacccg ggtggtcgag gtgctgctgg ccaccgtccg gccggtgcag
     1801 ctgctggccg gcaagatcat cggcatcggc ctggtcggcc tgctccagct ggccgtcatc
     1861 ggcgcggtcg ggctggccgt ggccgtggcc agcaacgcca tcaccctgcc gccggacgcg
     1921 gccgggacga tcggctcggt gctgctgtgg ttcctgctcg gctacgcgtt ctactcgagc
     1981 atgttcgcgg tggccggggc gatcgtgtcc cgccaggagg agctccagaa caccgccgcc
     2041 ccgctgaacc tcctcatgat cgcgtcgttc ttcgtcgcct tctcgagctc ggtgggcggc
     2101 ggggactcga ccctggccaa ggtgtcgtcg ttccttcccc cggtcgcccc gctggtcatg
     2161 ccggtccgga tcgccggcgg ggacgccgcc ctgtgggaga tcggcctctc cctggtgatc
     2221 atgctggtgt ccaccgtggc cgtggtcgtg ctggccgccc gcctgtacga gggggcgatc
     2281 ctgcgcacgg gggcgcgggt gaagctccgc gacgcctggc gcggcgcccg ccgccagccc
     2341 gccgccggcc gcgcctgacc ggtggtcggc accgcccacc tgctcagccc ggtcgggtgg
     2401 ccggccggaa ctcggtggtg ctggagacga ccccgtgggc gatgaccggc gcggtcacgg
     2461 cgctcgcgcg gccgctggaa cgaggtccgg ttgcccgcaa atccctcaat cgttgtcgtc
     2521 gaggaggccg gcgtcgtggg ccagcagggc gatctggacg cggttgttga ggtccagctt
     2581 ggtgaggatg ctcgagacgt gggtcttgac ggtggggacg ctgaggtaga gcacggcgcc
     2641 gatctcggcg ttggagcggc cctggccgac ggcgacggcg acctcgcgct cgcggtcgtt
     2701 cagcagggcc aggcgttccc gggcccgggt gcggcggtcg tggtcggccc cggcgacccg
     2761 ggagatgagc cgccgggtca cggccgggga cagcaccggc aggccctggg cgacccggcg
     2821 gacggcgtcc acgatctcgg ccggcggggt gtccttgagg acgaacccgg ccgcgccggc
     2881 gcgcagggcc cgcaggacgt gctcgtcggc gtcgaaggtg gtcaggatga tgacctccgg
     2941 cgcgttgcgg cgggcccgca gggcctcggt ggcggccagg ccgtccatgg ccggcatccg
     3001 gatgtccatc aggaccacgt cgggccggtg ccggtcgacc atggccggga cctgggcgcc
     3061 gtcgcccgcc tcgccgacca cgcgcaggtc gggggcgccg cccagcatca tcatcaggcc
     3121 ggcgcggacc agcgggtcgt cgtcgacgac gagcacgccg atgggctggg cctcggacgc
     3181 ggtcatggtg gccagggtag ccatgccgcc agccgccagc cgcccccgcc ggtcggcccg
     3241 tgctccagcc gcccgttggc cagggcgacc cgctcggcca gcccgaccag cccctgcccg
     3301 gccccggggt ccttcgcggg cccggggccg gaggcgggac cgggcaccgg ctggttgcgg
     3361 acctcgaccg tgaggccgtt gccgggcgac ccggccaggt ggaccagcac ctcggcgccg
     3421 ggggcgtgct tgcgggcgtt ggtcagggcc tcctgcacga cccgataggc ggtgcgcgcg
     3481 gccaggtcgg ggacggcgcc gctggtctcg tcgcgcaggg cgacccgcat cccggcccgg
     3541 cccgactcgg cgacgagctg ggcgacgtcg gcgagggtcg gctggggcag ctcgccgacc
     3601 ggggcgcgca ggacgccgat gacctcacgc aggtcctgga gcgcctggtg ggcgttctcc
     3661 cggatcaccg tggcggcgcg ggcgacctcc tcggcggggg cgtcgggccg gaactcgagc
     3721 gcgccggcgt ggacgctcag cagcgacagc cggtggccga gcacgtcgtg catctcgcgg
     3781 gcgatctcgt cccgggcgcg ctgctgggcc tgctcggccc gcagctgcgc ctcggtctcg
     3841 gcccggaccg cccggtcgcg cagcgactcc acgagctgg
//
LOCUS       DASSWR010000438         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1232413, whole genome shotgun sequence.
ACCESSION   DASSWR010000438 DASSWR010000000
VERSION     DASSWR010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232413"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFO47_07330"
     CDS             complement(<1..447)
                     /locus_tag="VFO47_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8788543.1"
                     /translation="MSAPPTPRAPGPGALGFGSVDELEEALAGHAYLADRGLATAIYL
                     ALHLDKPLLLEGEAGVGKTEVAKVLSDLLGRELVRLQCYEGIDASQALYEWDYSRQLI
                     AVRAAQLGEGQADLFGREFLVERPLLAAVRAGWRAVLLIDELDRADD"
     gene            complement(444..1271)
                     /locus_tag="VFO47_07335"
     CDS             complement(444..1271)
                     /locus_tag="VFO47_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8788544.1"
                     /translation="MIPAAFDYTVAESADHAVSLLGEHGEDAKLLAGGHSLLPLMRLR
                     LAAPSVLIDIGRIGDLNYIRDGGDHVAVGALTTMEEAHFSELLLRDCPILAHTAGEVG
                     DPQVRHRGTVGGTVAHSDPASDIPTVLLALDATLVVKGPDGERTVPAADFWTGIFESA
                     LGPQDLLTEIRVPKLAGAGWNYQKFHPRAQDWAIVGVAAVASNGGVKVALTNMGEKPL
                     RASAVEQALASGSDPEAAADQVLDGTSPPNDALASSEYRSALAKVLTRRAIEGARSS"
     gene            complement(1268..>2397)
                     /locus_tag="VFO47_07340"
     CDS             complement(1268..>2397)
                     /locus_tag="VFO47_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8788545.1"
                     /translation="RREQQANNGRRDGKLLGVGFSTYIEMCGLAPSQVLAALKYGAGG
                     WEAATIRCHPTGKVTVITGTSPHGQGHVTTWSQITADALGVDPDDVDVLHGDTAVSAL
                     GMDSYGSRSASVGGEALVRAAEKIRAKVRTIAAHELEVAEEDLEWKDGKFQVKGAPDR
                     AKSVPEVALTAWTAHALPADVEPGLEATAVYDPPNFTFPSGAHVCVVEVDPETGSTRI
                     DKYVAVDDCGTVINPMIVDGQVMGGVAQGIAEALYEEALYDENGTLLTGNMSTYRIPS
                     PAELPGYILDREVTPSTTNNLGIKGIGEAGTIASPPAVLNAVADALRFVGVEFIDKPA
                     SAEKVWRVVQEARGRAPGERVAEGGGTGAASMTPEEEGGTA"
BASE COUNT          293 a          867 c          885 g          352 t
ORIGIN      
        1 gtcgtcggcc cggtcgagct cgtcgatcag cagcaccgcc cgccagccgg cccgcaccgc
       61 ggccagcagc ggccgctcga ccaggaactc gcgcccgaac aggtcggcct ggccctcgcc
      121 gagctgggcg gcccgcacgg cgatgagctg gcgcgagtag tcccactcgt agagggcctg
      181 gctggcgtcg atcccctcgt agcactgcag gcggaccagc tcgcggccga gcaggtccga
      241 cagcaccttg gcgacctcgg tcttgccgac cccggcctcg ccctccagca gcagcggctt
      301 gtccaggtgc agggccaggt agatggcggt ggccaggccc cggtcggcca ggtaggcgtg
      361 gccggccagg gcctcctcga gctcgtcgac agaaccgaag ccgagggcgc cgggacccgg
      421 cgccctcgga gtgggtgggg cgctcagctc gaccgtgccc cttcgatcgc ccgcctggtc
      481 aggaccttgg ccagggcgct gcggtactcg gagctggcca gggcgtcgtt cggagggctg
      541 gtgccgtcca gcacctggtc ggccgcggcc tcagggtcgg accccgaggc cagcgcctgc
      601 tcgaccgcgc tggcccgcag gggcttctcc cccatgttgg tcagggccac cttgaccccg
      661 ccgttgctgg ccaccgcggc cacgcccacg atcgcccagt cctgggcgcg ggggtggaac
      721 ttctggtagt tccagccggc gccggccagc ttgggcaccc ggatctcggt gagcaggtcc
      781 tggggaccga gggcggactc gaagatgccc gtccagaagt cggccgccgg caccgtccgc
      841 tccccgtccg gccccttgac gaccagggtc gcgtcgaggg cgagcagcac cgtcgggatg
      901 tcggaggcgg ggtcggagtg ggccaccgtg ccgccgacgg tcccccggtg gcgcacctgg
      961 gggtcgccga cctcgccggc ggtgtgggcc aggatcgggc aatcgcgcag gagcagctcc
     1021 gagaagtggg cctcctccat ggtggtcagg gcgccaacgg ccacgtggtc gccgccgtcg
     1081 cggatgtagt tgaggtcccc gatgcggccg atgtcgatca gcaccgacgg ggcggccagc
     1141 cgcagccgca tcagcggcag cagcgagtgc ccgccggcca gcagcttggc gtcctcgccg
     1201 tgctcgccga ggaggctgac ggcgtggtcg gcggactcgg cgaccgtgta gtcgaacgct
     1261 gccgggatca cgccgtccct ccttcctctt cgggggtcat actggccgcc ccggtgccgc
     1321 cgccctcggc gacgcgctcc cccggcgccc tgccccgcgc ctcctggacg acccgccaga
     1381 ccttctccgc cgaggccggc ttgtcgatga actcgacccc gacgaagcgc agcgcgtcgg
     1441 cgaccgcgtt cagcaccgcc ggcggggagg cgatcgtccc ggcctcgccg atccccttga
     1501 tgccgaggtt gttggtggtc gacggggtga cctcgcggtc gaggatgtac cccgggagct
     1561 cggccgggga cgggatccgg taggtcgaca tgttgccggt cagcagggtg ccgttctcgt
     1621 cgtagagcgc ctcctcgtac agcgcctcgg cgatgccctg ggcgaccccg cccatcacct
     1681 gcccgtcgac gatcatcggg ttgatgaccg tgccgcagtc gtccacggcc acgtacttgt
     1741 cgatccgggt cgacccggtc tcggggtcga cctcgaccac gcacacgtgg gcgccggacg
     1801 ggaaggtgaa gttgggcggg tcgtagacgg ccgttgcctc caggcccggc tcgacgtcgg
     1861 ctggcagggc gtgggcggtc caggcggtga gggcgacctc cgggaccgac ttggcccggt
     1921 cgggcgcgcc cttgacctgg aacttgccgt ccttccactc gaggtcctcc tcggcgacct
     1981 cgagctcgtg ggcggcgatg gtgcggacct tggcccggat cttctcggcc gcccggacca
     2041 gggcctcgcc gccgaccgag gcgctgcggc tgccgtagga gtccatcccg agcgcggaaa
     2101 cggccgtgtc gccgtggagc acgtcgacgt cgtcggggtc gaccccgagg gcgtcggccg
     2161 tgatctggga ccaggtggtc acgtgtccct ggccgtgggg cgaggtgccg gtgatcaccg
     2221 tcaccttccc ggtcgggtgg cagcggatgg tggccgcctc ccagccgccg gctccgtact
     2281 tgagcgcggc caggacctgg gagggcgcca ggccgcacat ctcgatgtag gtcgagaagc
     2341 cgaccccgag cagcttgccg tcgcggcggc cgttgttggc ctgctgctcg cgccgga
//
LOCUS       DASSWR010000439         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_356118,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000439 DASSWR010000000
VERSION     DASSWR010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356118"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1562)
                     /locus_tag="VFO47_07345"
     CDS             complement(24..1562)
                     /locus_tag="VFO47_07345"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET8788546.1"
                     /translation="MDWFDDSALTLAVFLPLLGAVAVAVLPRERDGLIRGAALSFTAL
                     ALLVGVGMLARFDYEAGAAMQFEVSRDWIPAIGAGYHVGVDGIALPLLVLSLLLSLLC
                     VVYSWRILPSPRNPKAFLALLLLLETGMNGTFAALDLVLFFVFWETVLIPMYFLIAVW
                     GGARREYASIKFILYTLIGSVVMLLGFLALWLRSSPEAAGRTFDILALQELGAARFGG
                     TFGTIVFAAVALGFAVKVPVWPLHTWLPDAHTEAPTVGSVLLAGILLKMGTYGFVRIA
                     LPILPEAARTWAPAIGVLGAIAIIYGALCCLAQRDVKRLIAFSSVGHMGFVMLGIATL
                     TPTGINAAVFGMVAHGVITGMLFFLAGSMHERYHTRDIVELGGGMATLMPRLAGVFTL
                     ACIASLGLPGLAGFWGEILALLAAWNPAPGLSVPLFRTLALVGLVGTLLTAGYFLWLI
                     QRVNLGRVPDRWGGRALGDLAPLEAAAWSPLILLTVALGFAPGLILWLTSPSVTGWFT
                     DLFA"
     gene            complement(1584..>2658)
                     /locus_tag="VFO47_07350"
     CDS             complement(1584..>2658)
                     /locus_tag="VFO47_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET8788547.1"
                     /translation="GSVLNEIGILASITMLIAALLALVQDDIKRVLAYSTVSQLAYMM
                     AALSVGGYSAGIFHLFTHAFFKALLFLAAGAIIHAVHSNNMSEMGGLARAMPVTFVTF
                     LVGALALTGIAPLAGFWSKDEILTDAWNAGFAGAGEAATSQSVAQIVFVTGMVTAFIT
                     ALYVGRMLWLTFGAAYRGQGRPHEADAAMLFPLVVLALGAALAGFVGLGDNSIATWIQ
                     TEALPAHGPATLNLGLIVTTTVVGLAGLALGTLLYRRGLPEHEYLERVPVVWKTLQRK
                     YFLDDLYEQGVVRAVAGALAPATYWFDQRVVDGVVNGAGLGTRRLARGLRYVQSGQAQ
                     WYAAALFLGVIGLAVVVTQVIGG"
BASE COUNT          471 a          893 c         1001 g          293 t
ORIGIN      
        1 tggccacggt cagggtctcc tactcatgcg aacaggtcgg tgaaccagcc ggtgaccgag
       61 gggctggtca gccacaggat gaggccgggg gcgaagccga gggccacggt gaggaggatc
      121 aggggggacc aggcggcggc ctccagcggg gccaggtcgc caagggcccg gccgccccag
      181 cggtcgggga cccggcccag gttgacccgc tggatcagcc agaggaagta gccggcggtc
      241 agcagggtcc cgaccaggcc gaccagggcc agggtccgga acagcggcac cgacagcccc
      301 ggggccgggt tccaggcggc cagcagggcc aggatctcgc cccagaaccc ggccagcccg
      361 ggcaggccaa gggaggcgat gcaggccagg gtgaagaccc cggccaggcg gggcatgagg
      421 gtggccatgc cgccgcccag ctcgacgatg tcgcgggtgt ggtagcgctc gtgcatggag
      481 ccggccagga agaacagcat cccggtgatg acgccgtggg cgaccatgcc gaacacggcg
      541 gcgttgatcc cggtcggggt gagggtggcg atgcccagca tgacgaagcc catgtggccg
      601 accgaggaga aggcgatcag ccgcttgacg tcccgctggg ccaggcagca cagggccccg
      661 tagatgatgg cgatcgcccc gagcaccccg atcgccgggg cccaggtccg ggcggcctcg
      721 ggcaggatcg gcagggcgat gcgcacgaac ccgtaggtgc ccatcttcag caggatcccg
      781 gccagcagca ccgacccgac cgttggggcc tcggtgtggg cgtcgggcag ccaggtgtgc
      841 agcggccaca ccggcacctt gacggcgaag ccgagggcga cggcggcgaa cacgatcgtc
      901 ccgaaggtcc cgccgaagcg ggccgccccc agctcctgga gggcgaggat gtcgaaggtg
      961 cggccggcgg cctcggggct ggagcggagc cacagggcca ggaaccccag cagcatgacc
     1021 accgacccta tcagcgtgta gaggatgaac ttgatcgagg cgtactcgcg cctggccccg
     1081 ccccagacgg cgatcaggaa gtacatgggg atcaggaccg tctcccagaa gacgaagaac
     1141 agcaccaggt cgagggcggc gaaggtgccg ttcatgcccg tctcgagcag cagcagcagg
     1201 gccaggaagg ccttggggtt gcgcggggag ggcaggatcc gccacgagta gacgacgcac
     1261 agcaggctga gcagcagcga cagcaccagc aggggcaggg cgatgccgtc gacgccgacg
     1321 tggtagccgg ccccgatcgc cgggatccag tcccgggaca cctcgaactg catggccgcc
     1381 cccgcctcgt agtcgaagcg ggccagcatg cccaccccga ccagcagggc gagtgcggtg
     1441 aagctgagcg ccgccccccg gatcaggccg tcccgctccc gggggagcac ggcgacggcg
     1501 accgcgccga gcaggggaag gaacacggcc agggtcaggg ccgagtcgtc gaaccagtcc
     1561 atgccgagca cgcctccttc cggctagccg ccgatcacct gggtcaccac cacggccagg
     1621 ccgatcacgc cgaggaacag cgcggccgcg taccactggg cctgcccgga ctgtacatag
     1681 cggaggccac gggcgagccg tctcgtcccc agccccgccc cgttgaccac gccgtcgacc
     1741 acccgctggt cgaaccagta ggtggcgggg gccagggcgc cggccaccgc ccggacgacg
     1801 ccctgctcgt acaggtcgtc gaggaagtac ttgcgctgca gggtcttcca cacgaccggg
     1861 acccgctcca ggtactcgtg ctcgggcagg ccgcgccggt acaggagggt cccgagggcc
     1921 agaccggcca ggccgaccac ggtggtggtc acgatcaggc cgaggttgag ggtcgccggc
     1981 ccgtgggcgg gcagggcctc ggtctggatc caggtggcga tcgagttgtc gccaaggccc
     2041 acgaacccgg ccagggcggc cccgagggcg agcacgacca gcgggaacag catggccgcg
     2101 tcggcctcgt gcgggcggcc ctgcccgcgg taggcggccc cgaaggtcag ccacagcatc
     2161 cggcccacgt acagggccgt gatgaaggcg gtgaccatgc cggtgacgaa cacgatctgg
     2221 gccacgctct ggctggtcgc ggcctccccg gcgccggcga agccggcgtt ccaggcgtcg
     2281 gtgaggatct cgtccttgga ccagaacccg gccagcgggg cgatcccggt gagggccagg
     2341 gcaccgacca ggaaggtcac gaaggtgacg ggcatggccc gggccagccc gcccatctcg
     2401 ctcatgttgt tggagtggac ggcgtggatg atcgcgccgg cggccaggaa cagcagggcc
     2461 ttgaagaagg cgtgggtgaa caggtggaag atcccggccg agtacccgcc gacgctgagg
     2521 gcggccatca tgtaggccag ctgggagacg gtcgagtagg ccagcacccg cttgatgtcg
     2581 tcctggacca gggccagcag ggccgcgatc agcatggtga tgctggccag gatgccgatc
     2641 tcgttcagca ccgacccg
//
LOCUS       DASSWR010000440         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1088661, whole genome shotgun sequence.
ACCESSION   DASSWR010000440 DASSWR010000000
VERSION     DASSWR010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088661"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..657)
                     /locus_tag="VFO47_07355"
     CDS             complement(325..657)
                     /locus_tag="VFO47_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788548.1"
                     /translation="MILDERASQIADVLQDALGAGFHVAAFPPADDVIAVLTAASPRL
                     VDAHRQLHPTVGLVITGAGAQPDAVATVLNAGADDCLRETDPRELAARLKALSRRRAL
                     ISVHHPYS"
     gene            1223..2107
                     /locus_tag="VFO47_07360"
     CDS             1223..2107
                     /locus_tag="VFO47_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8788549.1"
                     /translation="MARAVLPAVVELQVSSAGQQGTGSGVVLRSDGYILTNAHVVEGA
                     TQITVTLQSGERVDAELVGSDATDDLAVVRVAKTGLQAATVGRSASLAVGQRVIAVGS
                     SFGLEGSVTEGIVSALHRVVLTGGPGQDSSDLIDAIQTDAAINPGNSGGALADDRGRV
                     IGITTAIESSSGANAGVGFAVPIDRAVEVADQLIAGRQVTVSYLGVTGGAEGDDGGSQ
                     GARIQQVSPDGPAADAGLRSGDLVTRVGDRTVRSWEDLVLAIRQLEAGSTAKVRVRRG
                     SAEQTLQVTVGSRPVDSR"
     gene            2238..>2466
                     /locus_tag="VFO47_07365"
     CDS             2238..>2466
                     /locus_tag="VFO47_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788550.1"
                     /translation="MTTPMPDPVIRTRALARTYLRGAIAVPVLEAVDLELAEGEWLAV
                     LGPSGSGKSTLLNILGLLDRPNAGVYELAGED"
BASE COUNT          346 a          833 c          921 g          366 t
ORIGIN      
        1 ccacactccg cgactcgacc atcgatcggg ctcgtgctgt gctgcattcg ctccttaatc
       61 ggaccccgga ccctcgactg ttccgtcgac ccgtgtcctt ctccagacat gcgaatccaa
      121 ggcgcggctg ccggccgggg gcggcgctgg tgcgtgctgt ttggcagggt cggcagtccg
      181 ctttcttgca cccggaatcc gagaactccg gcccaacgcc agaccgacga cggcggctgg
      241 ccgaccgagg gcggcgtcgg gggggggaga gagagagaca ccgccctcgg ccttctttgg
      301 tgctgtgccg gccgcccttg agcgttagct gtatggatga tgaaccgaga tcaaggcgcg
      361 gcgtcggcta agggctttga gcctggccgc cagctcgcgc gggtcggttt cccgcaggca
      421 gtcgtcggcg ccggcgttga ggaccgtggc caccgcgtcg ggctgggcgc ccgccccggt
      481 gatgaccagc ccgacggtgg ggtgcagctg gcgatgggcg tcgaccagac gcggggaggc
      541 cgccgtcagc acggcgatga catcgtcggc aggtgggaag gcagccacat ggaagccggc
      601 accgagcgcg tcctggagga cgtcggcgat ctggctggct cgctcgtcca ggatcacggt
      661 gacacgacgc tcgggtgcgg tctcggcttc gggttgcttg tcggctgatg cccatggcgc
      721 acgcatcgtc ggcctcgctt gtggctggta ggtgtcgacc gggccgagtg tcgctggcgg
      781 accttggagc agcctggcag tcggctgtgg atcggctggc aatcctggga gctcggccca
      841 tggttcacag caagctcaca ggttgcccga agggtgcccc aaggtccggt gtccagactc
      901 cggccgaccg tccagttcga ccgggagcgc caccatggag cagcagcgca cgttggggcc
      961 tttgccactg cagcggccac cagtcgacag tccgcgaccg cccgggcggt cgcggaccgt
     1021 ccttgtcgcc ggggcagttg ctctcgcgct cgtcgcgggg gcttctggtg gcctgatcgt
     1081 gcaggcgctg gaccgccagg gtggcaccga acccgttaca tcgccggcgc catcggcggt
     1141 gacgcccctg caggaggaca ccggctcggg cggcggcggc tcgaccggag cgtcagaaag
     1201 ctcggcgtcc gacgcgaagg cggtggctcg ggcggtgctc ccggccgtgg tcgagctgca
     1261 ggtcagctcg gctggccagc agggcaccgg gtcgggggtg gtgctccgct ccgacggcta
     1321 catcctgacc aacgcccacg tggtcgaggg agcgacccag atcaccgtga ccctccagag
     1381 cggcgagcgg gtggacgccg agctcgtcgg ctcggacgcc accgatgact tggccgtggt
     1441 ccgggttgcc aagaccgggc tgcaggccgc caccgtgggc cgttcggcca gcttggcggt
     1501 gggtcagcgg gtgatcgcgg tcggcagctc cttcggcctg gagggctcgg tcaccgaggg
     1561 catagtctcc gccctgcatc gggtggtcct gaccggcggt ccaggccagg acagcagcga
     1621 cctgatcgac gccatccaga ccgacgccgc catcaaccct ggcaactcag gtggggcgct
     1681 ggccgacgac cgcggccggg tgatcgggat caccaccgcg atcgagtcca gcagcggcgc
     1741 caacgctggg gtcgggtttg cggtccctat cgaccgggcc gtcgaggtcg ccgaccagct
     1801 catcgccggc cgccaggtca ccgtctccta cctcggggtc accggcgggg ccgaaggcga
     1861 cgacggcggc agccagggcg cccgcatcca gcaggtcagc cctgacggtc cagccgccga
     1921 cgctggactg cgatccggcg acctcgtcac ccgggtgggc gaccgcaccg tgcggagctg
     1981 ggaggacctg gtcctggcga ttcgccagct ggaggcgggc agcacggcca aggtccgagt
     2041 gcggcgcggc agcgccgagc agaccctgca ggtgacggtg ggcagccgcc cggtggacag
     2101 ccgatgagcc cgcccccagc gccagcggac cagccgggtt ctcagggaac tcccagccgg
     2161 tggacaggct cttccaaggt cggcccgcga gagtgccgga tcagtcagcg gcaaccaagc
     2221 ctggaggagg cacgcccgtg accacgccca tgcctgaccc ggtgatccgc acccgcgcac
     2281 tggcccggac ctacttgcgt ggcgcgatcg ccgtgccggt gttggaggcg gtcgacttgg
     2341 agctggccga gggcgagtgg ttggccgtcc tgggcccgtc ggggtcgggc aagtcgaccc
     2401 tgctgaacat tctcgggttg ctggaccggc ccaacgccgg ggtctacgag ctggccgggg
     2461 aggacg
//
LOCUS       DASSWR010000441         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_949174,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000441 DASSWR010000000
VERSION     DASSWR010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949174"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFO47_07370"
     CDS             complement(<1..312)
                     /locus_tag="VFO47_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET8788551.1"
                     /translation="MTAAAWERTLRVGTADETHALAAVLGAQLQAGDLLVLTGELGAG
                     KTTFTQGLGEGLGVRAGIISPTFVLVRIHPNLPGGPRPGGPDLVHVDAYRLGSASEID
                     DI"
     gene            complement(309..1529)
                     /gene="alr"
                     /locus_tag="VFO47_07375"
     CDS             complement(309..1529)
                     /gene="alr"
                     /locus_tag="VFO47_07375"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET8788552.1"
                     /translation="MTYPAATGDFRPVPQAPPAYAEERSAIIDLEAIRHNVRRLAAVA
                     SPAQVMAVVKADAYGHGAVPVARAALAAGAGWLGTAHISEALALRAAGIEAPLLAWLH
                     TPESNFAAAVAAGIDIGCSGWELERIVAAAREQERPARVHLKVDTGLGRNGATLEAWD
                     SLVGEAMDFQDQGLLRVVGIFSHLAVADEPERPETDDQLAAFRDALAIAQDAGVDPEV
                     RHLANTPATLSRPDTHFDLVRVGLGLYGLSPFDGQSSEELGLRPAMTVRTVVSHCKDV
                     PAGQGVSYGLNYHTGGPSTLGLIPLGYADGVPRIATGGPVRVGSVNYPVVGRIAMDQM
                     VIDLGPLNVSAGGPSVLGAEAVLFGNGDDGGPTAEDWARAAGTNNYEIVTRISPRVPR
                     RYINEEPASGEPRP"
     gene            complement(1694..>2623)
                     /locus_tag="VFO47_07380"
     CDS             complement(1694..>2623)
                     /locus_tag="VFO47_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET8788553.1"
                     /translation="MGSCPGGVANQAIAAARLGLRTGLAAAFGDDGYGDSNWKILAGQ
                     EHIDLSLSRKIAGWHSPVTVSLSVEHDRSMVTHGHPAPVSSSELVGCPPPALAAVAEL
                     GEELEPWALDAHKAGVRLFGDVGWDPTGVWSSARLDNLRYFHAFMPNQHEAMAFTGHD
                     NPWAALYALADKVPVAVVTLGPQGAMAVDSETGEEEWVPSLPVTAYDPTGAGDCFGAA
                     FIVGCLAGWPLGDRLRFANLCAALAVQEVGGSLAAPGWGDIADWWQRANARKERQSSQ
                     WLRRYSFLADIVRDVPPEAQRRATATIAHLSDA"
BASE COUNT          361 a          922 c          967 g          373 t
ORIGIN      
        1 gatatcgtcg atttccgagg ccgaacccag ccggtacgca tccacgtgca ccaggtccgg
       61 gccgccgggc cgcggacccc cgggcaggtt cgggtggatc cggaccagca cgaaggtcgg
      121 tgagatgatg ccggcacgca cacccaggcc ctcgccgagg ccctgggtga aggtggtttt
      181 ccccgcgccc agctcgccgg tgaggaccag caggtcaccg gcttggagct gggcgccgag
      241 gacagcggcg agagcatgcg tttcgtccgc ggtcccgacc cgcagggtcc gttcccaggc
      301 cgccgcggtc acggccgcgg ctcgccgctg gcaggctctt cgttgatgta gcggcgcggc
      361 acccggggac tgatgcgcgt gacgatttcg tagttgttgg tgccggccgc ccgggcccag
      421 tcctccgcgg tgggcccgcc gtcgtcgccg ttgccaaaga gcacggcttc ggcgccgagg
      481 acgctggggc cgccggcgga gacgttgagg gggcccaggt cgatcaccat ctggtccatc
      541 gcgatgcgtc ccaccaccgg gtagttcacg ctcccgacac gcaccgggcc tccggtggcg
      601 atgcggggga cgccgtcggc gtagccgagc gggatcagcc ccagcgtgct ggggccgccg
      661 gtgtggtagt tcaggccgta ggagacgccc tggccggccg gcacatcctt gcagtgcgac
      721 acgacggtcc ggaccgtcat ggcggggcgc agtcccagct cctccgagct ctgcccgtcg
      781 aacggcgaca gtccgtacag gccgagcccg acgcggacca ggtcaaagtg ggtgtcgggc
      841 cgggacaggg tggccggggt gttggcgagg tggcgcacct cggggtccac gccggcgtcc
      901 tgagcgatgg cgagggcatc gcggaatgcc gccagctgat catcggtttc cgggcgttcg
      961 ggttcgtcgg cgacggccag gtgggagaag atgcccacca cgcgcagcag gccctggtcc
     1021 tggaagtcca tggcttcgcc cacgaggctg tcccaggcct ccagcgtcgc cccgttgcgg
     1081 cccagcccgg tgtccacctt gaggtgcacg cgggccggcc gttcctgctc ccgggccgcc
     1141 gcaacaatgc gttccagttc ccagcccgag cagccgatat cgatgccggc ggccacggcg
     1201 gcggcgaagt tgctctccgg ggtgtgcagc caggccagca ggggggcctc gatgccggcg
     1261 gcgcgcaggg cgagggcctc ggagatgtgg gcggttccca gccagccggc accggcagcg
     1321 agcgcggcgc gggcgacggg gacggcgccg tggccgtagg cgtcggcctt gaccacggcc
     1381 atcacctggg ccggtgatgc gacggcggcc aggcggcgga cgttgtgccg gatggcttcg
     1441 aggtcgatta ttgccgaacg ctcctcggcg taggccggcg gagcctgcgg gacgggccgg
     1501 aagtcacctg ttgctgctgg gtaagtcact taacgattct aggggtggct gcgcatgcgg
     1561 ctgaattcgg gcctcccggc accacccctc ccccggggga catgcccagt gttcgagccc
     1621 gcgactgggc accatggcat catgtccact cctgggcccg cggggagcgc atgtccagcg
     1681 ggaggggcgc ctactacgcg tccgaaaggt gcgcgatggt cgccgtcgcc cgccgttgcg
     1741 cttccggcgg cacgtcacgg acgatgtccg cgaggaagct gtagcgccgg agccactggc
     1801 tgctctggcg ttccttccgg gcgttggccc gctgccacca gtcggcgatg tccccccagc
     1861 cgggcgcggc gagcgagccg ccgacctcct gcacggccag ggcggcgcag agattggcga
     1921 accgcagccg gtcaccgagc ggccagccgg ccaggcagcc gacgatgaag gcggcgccga
     1981 agcagtcccc cgcgccggtc ggatcgtagg cggtcaccgg cagcgacggg acccactcct
     2041 cttcgcccgt ttcggagtcc acggccatgg cgccctgcgg gcccagggtc acgacggcca
     2101 ccggcacctt gtccgccagc gcgtagagcg ccgcccacgg gttgtcgtgc ccggtgaagg
     2161 ccatcgcctc gtgctggttc ggcataaagg cgtggaagta ccgcaggttg tccagccgcg
     2221 ccgaggacca gaccccggtg ggatcccagc cgacgtcgcc aaacagccgg accccggcct
     2281 tgtgcgcgtc gagcgcccac ggctccagct cctcgccgag ctccgcgacg gcggcgagcg
     2341 ccggcggagg gcagccgacc agttcgctgg agctcaccgg cgcgggatgg ccgtgcgtca
     2401 ccatggaccg gtcgtgctcc acgctgaggg acaccgtgac cggggagtgc cagccggcga
     2461 tcttccggga cagcgacagg tcaatgtgtt cctgtccggc caggatcttc cagttggagt
     2521 cgccgtagcc gtcatcaccg aacgctgccg cgagcccggt ccgcagcccc aaccgtgccg
     2581 cggcgatggc ctggttggcc acgcccccgg ggcagctgcc cat
//
LOCUS       DASSWR010000442         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_610198,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000442 DASSWR010000000
VERSION     DASSWR010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610198"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFO47_07385"
     CDS             complement(<1..710)
                     /locus_tag="VFO47_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8788554.1"
                     /translation="MTSTPTSTRTVRDWRLWLALATVYLVWGSTYLAIRVMVETIPPL
                     LGSGVRFLLAGAVLYPFLLARRGWTGVRVPARQVGASALVGLLFLAGGTGLVTVAERD
                     VPSGLAAVLVAAMPLVVVLLRRLAGERVPRATALGVAAGFAGVGVLVLPGNPAPEAGW
                     FGLVLILVANVSMAVASFASSRLPVPGDTLVATALELLAAGVALCLGGLLAGEAAHLE
                     LQRLSGASLAALAYLVVAG"
     gene            781..1695
                     /locus_tag="VFO47_07390"
     CDS             781..1695
                     /locus_tag="VFO47_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8788555.1"
                     /translation="MLDVRRLRVLREVSLRGSFSAAAQALAYSQSAVSQQIAALEREA
                     GTRLVERHGRGVRLTDAGQALVRRADAILAELAAADAELAAIAGLRGGRVRVSTFASA
                     ATALLPAAVAAFRAGHPEVQVELSLVEATDEAVDGVRAGRADLALLTQPVDQPPPDLV
                     ETHRLFEDPMLVVLPGGHPLARRRALALADLAGEPWVLGGGPACSDRDTILRACHAAG
                     FEPRVTVAFPTDDYNATQGMVAAGAGITLLPRLALAVPRDDLAVRPLAGAGVSRLVVA
                     AVRRGDQAAATMAMLEVVREEGRRLSGS"
     gene            complement(1682..2863)
                     /locus_tag="VFO47_07395"
     CDS             complement(1682..2863)
                     /locus_tag="VFO47_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8788556.1"
                     /translation="MSEQPPASTDFYRIDDLLTDAEREVRDRVRTFCDKEVTPIINDY
                     WERAEFPFELVPKLAALNLAGGTIEGYGCPGLSTVATGLVGMELGRADGSICTFFGVH
                     SGLAMTSINLLGSEEQKRRWLPAMARMEKIGAFGLTEPDHGSDAVALETRARRDGDHW
                     VLDGAKRWIGNASFADLVIVWARDDDGKVGGFVVEKGTPGYAASVMAGKTAKRAVWQA
                     EITLDGVRVPAENRLEHSRGFADTARVLTATRSGVAWEAAGHALAAYEAALAYVSDRE
                     QFGTPLASFQLVQYRLAKMLAEVTTMQLLCLRLAQLTDAGQMTAGMASLAKMNNAAKA
                     RQVVADARDVLGGNGILLENHVARHHADMEAVYTYEGTDAIQSLIVGREITGESAFTS
                     R"
     gene            2937..>3462
                     /locus_tag="VFO47_07400"
     CDS             2937..>3462
                     /locus_tag="VFO47_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET8788557.1"
                     /translation="MTHDIRSVAVLGAGTMGAAIAAHAANAGLAVDLLDLDSDAVHGG
                     FKRMLAARPAALASPRLAERIRLGTFEEDFDRVAAADWVVEAIVERLEPKQELFARVE
                     KTAGPGAVVTSNTSGLPLASLAEGRSDDFRRRFLGTHFFNPPRYLKLVELIPTGDTDR
                     AIMERMARFCERVLG"
BASE COUNT          444 a         1290 c         1286 g          442 t
ORIGIN      
        1 ccggctacga ccaggtaggc gagcgcggcc agggacgcgc ccgagaggcg ctgcagctcc
       61 aggtgggcgg cctcgccggc aaggaggcca ccgaggcaga gcgcgacccc ggcagccagc
      121 agctccagcg cggtggcgac cagggtgtcg ccggggacgg gcagccggct ggaggcgaac
      181 gacgccaccg ccatcgacac gttggccacc aggatgagga ccaggccgaa ccagccggcc
      241 tcgggtgccg ggttgccggg cagcacgagc acgccgacgc cggcgaaccc ggccgcgacc
      301 ccgagggcgg tggcgcgggg gacgcgctcg ccggccagcc ggcgcagcag caccacgacc
      361 agcggcatgg ccgcgaccag caccgccgcc agccccgagg ggacgtcccg ctcggcgacg
      421 gtgaccaggc cggtcccgcc ggccaggaac agcaggccga ccagggcgct ggccccgacc
      481 tgccgggccg gcacccggac cccggtccag ccccggcggg ccagcaggaa cgggtagagg
      541 acggccccgg ccagcaggaa ccggaccccg gagccgagca gcggcgggat cgtctccacc
      601 atcaccctga tcgccaggta ggtcgagccc cagaccaggt agacggtggc cagggcgagc
      661 cacagccgcc agtcgcggac ggtccgggtc gaggtcggtg tggaggtcat gggagatggg
      721 tatccagcca atgccatcag ctcacatgac agttcctgcc cctaccgata agtggtgctt
      781 atgctcgacg tgcgccggct gcgggtgctc cgcgaggtct cgctgcgggg ctcgttctcg
      841 gccgccgccc aggcgctcgc ctacagccaa tcggccgtct cccagcagat cgccgccctc
      901 gagcgcgagg ccggcacccg gctggtcgag cgccacggcc gcggcgtccg cctgaccgac
      961 gccggccagg cgctggtccg ccgggccgac gccatcctgg ccgagctggc cgccgccgac
     1021 gccgagctgg cggcgatcgc cgggctgcgg ggcggccggg tccgggtgtc caccttcgcc
     1081 agcgcggcca ccgcgctgct gccggccgcc gtcgccgcct tccgcgccgg ccacccggag
     1141 gtccaggtcg agctctcgct ggtcgaggcg accgacgagg ccgtcgacgg ggtgcgggcc
     1201 gggcgggccg acctggccct gctgacccag ccggtcgacc agccgccccc cgacctggtc
     1261 gagacccacc gcctgttcga ggacccgatg ctggtggtgc tgccgggcgg gcacccgctg
     1321 gcccgccggc gggccctggc cctggccgac ctggccggcg agccgtgggt cctgggcggc
     1381 ggccccgcct gctcggaccg cgacaccatc ctgcgggcct gccacgccgc cgggttcgag
     1441 ccccgggtca cggtcgcctt cccgaccgac gactacaacg ccactcaggg catggtggcc
     1501 gccggcgccg ggatcacgct gctgccgcgg ctggccctgg ccgtgccccg cgacgacctg
     1561 gccgtgcggc ccctggccgg cgccggggtg agccggctgg tggtcgcggc cgtccgccgc
     1621 ggcgaccagg cggcggccac catggccatg ctggaggtcg tcagggagga gggccggcgg
     1681 ctcagcggct cgtgaaggcg ctctcgccag tgatctctcg cccgacgatc aagctctgga
     1741 tggcgtcggt gccctcgtag gtgtagacgg cctccatgtc ggcgtggtgg cgggcgacgt
     1801 ggttctcgag caggatgccg ttgccgccga ggacgtcgcg ggcgtcggcc accacctggc
     1861 gggccttggc ggcgttgttc atcttggcca gggaggccat gcccgcggtc atctggccgg
     1921 cgtcggtgag ctgggccagg cgcaggcaga gcagctgcat ggtggtcacc tcagccagca
     1981 tcttggccag ccggtactgg accagctgga agctggccag cggggtgccg aactgctcgc
     2041 ggtcgctgac gtaggcgagg gcggcctcgt aggcggccag ggcatggccg gccgcctccc
     2101 aggcgacccc ggagcgggtg gccgtcagca cccgggccgt gtcggcgaag ccgcgcgagt
     2161 gctccagccg gttctcggcc gggacccgga ccccgtcaag ggtgatctcg gcctgccaca
     2221 cggcccgctt ggccgtcttg ccggccatca cgctggccgc gtacccgggc gtgcccttct
     2281 cgaccacgaa cccgccgacc ttgccgtcgt cgtcgcgggc ccagacgatc accaggtcgg
     2341 cgaaggaggc gttgccgatc cagcgcttgg ccccgtccag cacccagtgg tcgccgtcgc
     2401 ggcgggcccg ggtctccagc gcgaccgcgt ccgagccgtg gtcgggctcg gtcagcccga
     2461 aggcgccgat cttctccatg cgggccatgg ccggcagcca gcgccgcttc tgctcctccg
     2521 acccgagcag gttgatcgag gtcatggcca ggccggagtg gacgccgaag aaggtgcaga
     2581 tgctgccgtc ggcccggccc agctccatgc cgaccaggcc ggtggcgacg gtgctgagcc
     2641 ccgggcagcc gtagccttcg atggtgccgc cggccaggtt gagggcggcc agcttgggca
     2701 ccagctcgaa ggggaactcg gcccgctccc agtagtcgtt gatgatcggg gtgacctcct
     2761 tgtcgcagaa ggtccggacc cggtcgcgga cctcgcgctc ggcgtcggtc aggaggtcgt
     2821 cgatgcggta gaagtcggtc gacgccggtg gttgctcgct catggccggg tccctccgct
     2881 gggtgcgctc cgcttcgagc gtaccctcgg aacgccgatc acctggaggt gcgtccttga
     2941 cccacgacat ccgttccgtc gccgtgctcg gcgccggcac catgggggcc gccatcgccg
     3001 cccacgcggc caacgccggc ctggccgtcg acctgctcga cctggactcc gacgccgtgc
     3061 acggcgggtt caagcgcatg ctggccgccc ggccggccgc cctggccagc ccccggctgg
     3121 ccgagcgcat ccgcctgggg accttcgagg aggacttcga ccgggtcgcc gccgccgact
     3181 gggtcgtcga ggcgatcgtg gagcgcctgg agcccaagca ggagctgttc gcccgggtcg
     3241 agaagacggc cggccccggg gcggtggtca cctccaacac ctccgggctg ccgctggcca
     3301 gcctggccga gggccgctcg gacgacttca ggcgccggtt cctcgggacc cacttcttca
     3361 acccgccccg ctacctcaag ctggtcgagc tgatcccgac cggcgacacc gaccgggcca
     3421 tcatggagag gatggcgcgg ttctgtgagc gggtgctcgg ca
//
LOCUS       DASSWR010000443         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_243301,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000443 DASSWR010000000
VERSION     DASSWR010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243301"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..468
                     /locus_tag="VFO47_07405"
     CDS             112..468
                     /locus_tag="VFO47_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET8788558.1"
                     /translation="MDRDAMLGYLGGRPGAVEDYPFGDDVAVFKVGGRVFAICDLTGQ
                     PGSVSLKCDPTLAEALRDRYPAVTPGYHLNKRHWNTVELDGSVPGDELAELVDHSWEL
                     VVAKLPRRDRDALAAG"
     gene            complement(465..1688)
                     /locus_tag="VFO47_07410"
     CDS             complement(465..1688)
                     /locus_tag="VFO47_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET8788559.1"
                     /translation="MRILLAAVNAPKGDLDGNLAGHLAVLDQARAQGCHLAVFPEFSL
                     TGSVDPRRHPGRALALDAEPVLTLLEGTRHAGVAALFGIAEHEGATFHITQVYGHSGR
                     LGGVYRKRHLGEGEEGYQTGEGPGVFQLGAARFGVTLCAEGEVDLPWTDAAAGGASVV
                     CFCSAPGLFGRRTDEQGWRDGHAWWVSAGLGDAIRHARRLGVPVAMATQAGTTEDEDF
                     PGLAALVSPSGEVARLPDWRPGSLVVEVAADVTVHPVREAVRCLVIDHAGRALLVRYA
                     DDRVGGTWWGPPGGGLDPGEDHLAAVRRELREELARDDLEVGPWIGRRCHTFWLGRWM
                     TQRERWVLCRAEPFEVDPAHVTTLAAEAIQELRWWSADELRVSGVVATPRDLPGLLDR
                     IARGRLPGPDQDLGI"
     gene            1891..3138
                     /locus_tag="VFO47_07415"
     CDS             1891..3138
                     /locus_tag="VFO47_07415"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788560.1"
                     /translation="MLVALGLSVAVAAVPAVASASIALGTVVSANPANFTPNVASGAV
                     HKFVQVGGTMYAGGAFSSVSTPAGVSPGGTFARSNIVAFNATTGVISSFAPNVNGEVW
                     ALASDGTSLWIGGTFTSVNGTARRAVAKLNRTTGAVDTAFNANLASGKVTELALVGGR
                     LLAGGTFPGKLRAVNPSTGANTGYLNLSISGSVTDNAGPVEVYRFAVNPAGTRLVAVG
                     NFTSVLGQTRYRAFMLTLGATASLNAWYYPPLQNLCRAGSLPDYMRDVDFSPDGSWFA
                     FVSTGYIPVSGGVGRDLCDATARFETNVTNPVRPTWINYTGGDTLHSVAATDVAVYVQ
                     GHQRWLNNPNGADTAGPGAVSRPGIGAINPATGLALSWNPTKERGVGGKDLYVTPQGL
                     WVGSDTNRIGGETRRRIALMPVP"
     gene            complement(3206..3721)
                     /locus_tag="VFO47_07420"
     CDS             complement(3206..3721)
                     /locus_tag="VFO47_07420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET8788561.1"
                     /translation="MSTLDRMVARLLVRWLREPVRQVLAEQDPRQAGPEAHVEMYKYL
                     VHGDRSKLDIHPTAVVNNALFNVGSGLITVGEYAFFGHNVSVLTGTHDINTFGRERQL
                     AIPRSGRDIVIEEGVWVASNAMVLGPCRIGAHAVVGGGSLVMDDVAPYTIVAGSPAKV
                     LRTIPHDGQPG"
     gene            complement(3830..4012)
                     /locus_tag="VFO47_07425"
     CDS             complement(3830..4012)
                     /locus_tag="VFO47_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8788562.1"
                     /translation="MAAGAGDRRRPADPPAWSEELAAGIPGARLVVVAGAGHLSPLDQ
                     PERVTEALVAWASGEE"
BASE COUNT          576 a         1599 c         1448 g          526 t
ORIGIN      
        1 cggctcaacc agaacatccc gcccgcgtga ccgcggcggc cggcgaggcc ggcaactccc
       61 ggtcctccac tccgccgacc ccggcgacct gcacctgatc gtggagggac catggaccgt
      121 gacgccatgc tgggctacct cggcgggcgg ccgggggcgg tcgaggacta cccgttcggc
      181 gacgacgttg ccgtgttcaa ggtcggcggg cgggtgttcg ccatctgcga cctgaccggc
      241 cagccgggga gcgtcagcct gaagtgcgac ccgaccctgg ccgaggcgct gcgcgaccgc
      301 tatccggccg tcacccccgg gtaccacctg aacaagcggc actggaacac ggtcgagctt
      361 gacgggtcgg tgcccggcga cgagctggcc gagctggtcg accactcctg ggagctggtg
      421 gtggccaagc tcccccgccg cgaccgcgac gcgctggccg ccggctagat cccgaggtcc
      481 tggtcggggc cggggaggcg gccgcgggcg atccggtcga gcaggccggg caggtcgcgg
      541 ggggtggcga cgacccccga gacgcggagc tcgtcagcgg accaccagcg cagctcctgg
      601 atggcctctg cggccagggt ggtcacatgg gcggggtcga cctcgaacgg ctcggcgcgg
      661 cacaggaccc accgttcccg ctgggtcatc caccggccca gccagaaggt gtggcagcgc
      721 cgcccgatcc acggcccgac ctcgaggtcg tcgcgggcca gctcctcccg cagctcgcgg
      781 cggaccgcgg ccaggtggtc ctcgcccggg tccagccccc cgcccggcgg gccccaccag
      841 gtgccgccga cccggtcgtc ggcgtagcgg accagcaacg cccgaccggc atggtcgatg
      901 accaggcagc ggacggcctc gcggactgga tggacggtga cgtcggcggc cacctccacg
      961 accaggctgc cgggccgcca gtcgggcagc cgggcgacct ccccggacgg ggacaccagg
     1021 gcggccagcc cggggaagtc ctcgtcctcg gtcgtcccgg cctgggtggc catggccacc
     1081 ggcacgccca gccggcgggc gtggcggatg gcgtcgccga gcccggccga gacccaccag
     1141 gcgtgcccgt cgcgccagcc ctgctcgtcg gtgcggcgcc cgaacaggcc gggggccgag
     1201 cagaagcaca ccaccgacgc cccaccggcg gccgcgtcgg tccaggggag gtcgacctcg
     1261 ccctcggcgc agagggtgac cccgaaccgg gccgccccga gctggaacac ccccgggccc
     1321 tcgccggtct ggtagccctc ctcgccctcg ccgaggtggc gtttgcggta gaccccgccg
     1381 aggcggccgc tgtggccgta cacctgggtg atgtggaagg tcgcgccctc gtgctcggcg
     1441 atcccgaaca gggcggcgac accggcgtgc cgggtcccct ccagcagggt caggaccggc
     1501 tcggcgtcga gggccagcgc ccgcccgggg tggcggcggg ggtcgaccga cccggtcagc
     1561 gagaactcgg ggaacacagc caggtggcag ccctgggccc ttgcctggtc gagcacggcc
     1621 aggtgcccgg ccaggttgcc gtcgaggtcg cccttcgggg cgttgacggc ggcgagcagg
     1681 atgcgcactc cgtccctcct gaccagcggc gggctgcctg gacgccggat gcagcgtagt
     1741 tcaaccgcgt gatccggctc ttgacggaac tgggtaggat ctgcgccgac atcccatcgt
     1801 gcccggccgg gcgccaacgc cggtgggaca cctctgggag cgaaggggac aaccgacgtg
     1861 gttccgttca gcaagcgcac gcttcgcacc atgctggtcg cgctcggcct cagcgtggcc
     1921 gtggccgccg tcccggccgt cgcctcggcg tcgatcgcgc tcggcaccgt ggtcagcgcc
     1981 aacccggcca acttcacccc gaacgtggcc agtggcgccg tccacaagtt cgtccaggtc
     2041 ggcgggacca tgtacgccgg cggagccttc agctcggtca gcaccccggc cggcgtcagc
     2101 cccggcggga ccttcgcccg cagcaacatc gtcgccttca acgccaccac cggggtgatc
     2161 agctcgttcg cgcccaacgt caacggcgag gtctgggcgc tggccagcga cggcacctcg
     2221 ctgtggatcg gcgggacctt caccagcgtc aacggcaccg cccggcgcgc ggtggccaag
     2281 ctcaaccgga ccaccggggc cgtcgacacc gccttcaacg ccaacctcgc ctccggcaag
     2341 gtcaccgagc tggccctggt cgggggccgg ctgctcgccg gcggcacctt cccgggcaag
     2401 ctgcgggccg tcaacccgag caccggggcc aacaccggct acctcaacct gtcgatcagc
     2461 ggcagcgtca ccgacaacgc cgggccggtc gaggtctacc gcttcgctgt caacccggcc
     2521 gggacccggc tggtcgcggt gggtaacttc acctcggtcc tgggccagac ccgctaccgg
     2581 gcgttcatgc tcaccctggg ggccacggcc tcgctcaacg cctggtacta cccgccgctg
     2641 cagaacctct gccgggccgg gagcctgccc gactacatgc gcgacgtcga cttctcgccc
     2701 gacgggtcgt ggttcgcgtt cgtgtccacc ggctacatcc cggtttccgg cggggtcggg
     2761 cgcgacctgt gcgacgccac cgcccggttc gagaccaacg tcaccaaccc ggtccgtccc
     2821 acctggatca actacaccgg cggcgacacc ctgcactcgg tggccgcgac cgacgtggcc
     2881 gtctacgtcc agggccacca gcgctggctc aacaacccca atggggccga caccgccggt
     2941 cccggcgcgg tcagccggcc cggcatcggc gccatcaacc ccgccaccgg cctcgccctc
     3001 tcctggaacc cgaccaagga gcgcggcgtc ggcggcaagg acctctacgt caccccgcag
     3061 ggcctgtggg tcggcagcga caccaaccgc atcggcggcg agacccgccg ccgcatcgcc
     3121 ctcatgccgg tcccctgacc gcccgctccc agccgctgac gtaggctcgc agaaggcggg
     3181 cgaaggtctc gcggtcctcg tccggtcagc cgggctggcc gtcgtggggg atggtccgca
     3241 gcaccttggc cggggagccg gccacgatcg tgtagggcgc gacgtcgtcc atgaccagcg
     3301 agcccccgcc gaccacggcg tgggcgccga tccggcacgg gcccagcacc atggcgttgc
     3361 tggccaccca gacgccctcc tcgatgacga tgtcgcgtcc ggagcgcggg atggccagct
     3421 ggcgctcgcg cccgaaggtg ttgatgtcgt gggtgccggt caggaccgac acgttgtggc
     3481 cgaagaaggc gtactcgccc accgtgatca ggcccgagcc gacgttgaac aaggcgttgt
     3541 tgaccacggc ggtcgggtgg atgtccagct tcgagcggtc cccgtggacc aggtacttgt
     3601 acatctccac gtgggcctcg ggcccggcct ggcgtgggtc ctgctcggcc agcacctggc
     3661 gcaccggctc ccgcagccag cggacgagca gcctcgccac catgcggtca agtgtcgaca
     3721 tccacgcctc ctgtccatcg ccatccccgc cggtccgccc gaagccgcct cagcatcgca
     3781 tccagggtga ccgacccgga agtcgggccc gcggccgccc ccggcggcgt cactcctcgc
     3841 ccgaggccca cgccaccagc gcctcggtga cccgctcggg ctggtcgagc gggctcaggt
     3901 gaccggcgcc ggccaccacg accagccgcg cccccgggat gccggcggcc agctcctccg
     3961 accacgccgg cgggtcagct ggtcggcgac ggtcaccggc cccagccgcc acagcgccgg
     4021 gagctgccgc tcgtacaacc gcagcgtgac cagcagcccg gggatcagca ggatcggaag
     4081 cggtccggcc atatgccggc ctccttgcgg ttcaggcctg tttcggaacg ctcgaggtcg
     4141 agccgcagc
//
LOCUS       DASSWR010000444         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_497185,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000444 DASSWR010000000
VERSION     DASSWR010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497185"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFO47_07430"
     CDS             <1..252
                     /locus_tag="VFO47_07430"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET8788563.1"
                     /translation="VFLSGGQSDEAATAHLNAMNRLGDAPWQLSFSYGRALQAPALKA
                     WKGEAANGQAAQRAYLHRARLNGAARSGSWTEAMETADT"
     gene            353..928
                     /locus_tag="VFO47_07435"
     CDS             353..928
                     /locus_tag="VFO47_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8788564.1"
                     /translation="MVQGLRELGAVDRAVYEAVARTPTGTLDDPVRRLSAAADKSKLW
                     LAIAAAVALAGGKRGRRAALEGVVAIGVSSATINLGVKQVARRRRPDRVRPALFENRH
                     VPMPGSTSFPSGHAASAFAFAYAVGRHLPGMAVPLRLLAAAVAYSRVHTGVHYPGDVV
                     IGSVAGSGTAAMVAAVADRAARSRAVSGRGR"
     gene            complement(909..1445)
                     /locus_tag="VFO47_07440"
     CDS             complement(909..1445)
                     /locus_tag="VFO47_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET8788565.1"
                     /translation="MFRRKARRDEPPEGAPAGTRYQLREKLLSVGDDFWIENDRGERI
                     FLVDDKVLRVRDTVVIKDAHGQELLKLQKRLLRARNTMVIERGDDKVATVRKAMITPL
                     RDRFTVDLEDGGRLEVQGNILDHEYQISRDGIPVANISKRWFRVRDTYGVAVVPGQND
                     ALVLAVTVCIDHLTDHDR"
     gene            1570..2292
                     /locus_tag="VFO47_07445"
     CDS             1570..2292
                     /locus_tag="VFO47_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HET8788566.1"
                     /translation="MAEAFGWGAIGAAALLVGALIAYLLAPGRRVIAVVMALGTGLLI
                     GSVAYELVDDALEHQAVVWVAAMVLVGALVFTGGDWLLDRGGGEDRKDATGAQADGSP
                     LAIVLGSVLDGIPESFVLGLTVLQGGVSVSLLAGVALSNLPEGMSSSSGLKAAGWPQG
                     RVLTMWSVVVLVSGVAAAAGYAMLGPASGRTGALVQAFAGGALLAMLADTLLPQAYAV
                     EGVLTGTLVVIGFAISLALSAA"
     gene            2300..>2875
                     /locus_tag="VFO47_07450"
     CDS             2300..>2875
                     /locus_tag="VFO47_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788567.1"
                     /translation="MATKRSRMRERREQLEAMLVTGQARLERARSRSMVVDTMVGVVR
                     RERPVAVGILAASLAFRLFAMLVPLCYVLVAGLGFAADRAAEEASPRGGNRLGDLVIA
                     SVAAATRTSGQGRWLALILGALATLIAAAAVLEVLRWVHLLAWRMAPARGHVSRWLVL
                     GLVTGLAVASFTATLAEQARADAKGLATEVTV"
BASE COUNT          306 a         1089 c         1062 g          418 t
ORIGIN      
        1 gtgttcctgt ccggcggcca gagcgatgag gcggccaccg cccacctcaa cgccatgaac
       61 cggctcgggg acgccccctg gcagctgagc ttctcctacg gccgggccct ccaggccccg
      121 gccctcaagg cctggaaggg cgaggcggcc aacggccagg cggcccagcg ggcctatctc
      181 caccgggccc ggctgaacgg ggccgcccgg tcgggcagct ggaccgaggc catggagacc
      241 gccgacacct aatccggagg tgcccgaggt gacagccccc ggcccgagcg acaccgtcgc
      301 cgacgccctg gcccggcggg tcacggcccg cgagggcccg gcggccaacg tcgtggtcca
      361 ggggctccgc gagctggggg cggtcgaccg ggccgtctac gaggcggtgg cccgcacccc
      421 caccggcacc ctcgacgatc ccgtgcgccg gctctcggcc gcggccgaca agtcgaagct
      481 gtggctggcc atcgccgccg cggtggccct ggccggaggc aaacggggcc gccgggcggc
      541 gctggagggg gtggtggcca tcggcgtcag ctcggccacg atcaacctgg gggtcaagca
      601 ggtcgcccgc cgccgccgcc ccgaccgggt ccggccggcc ctgttcgaga accgccacgt
      661 ccccatgccc ggctcgacct cgttcccctc gggccacgcc gcgtcggcgt tcgcgttcgc
      721 ctacgccgtc ggccggcacc tgccgggcat ggccgtgccg ctccgcctgc tcgccgccgc
      781 cgtcgcctac tcccgggtcc acaccggcgt ccactacccg ggcgacgtgg tgatcggctc
      841 ggtcgccggc tccggcacgg cggccatggt cgcggccgtg gccgaccgcg ccgcccgctc
      901 ccgggcggtc agcggtcgtg gtcggtgaga tggtcgatgc agacggtgac ggccagcacc
      961 agggcgtcgt tctggcccgg gaccacggcc acgccgtagg tgtcgcggac ccggaaccag
     1021 cgcttggaga tgttggccac cgggatgccg tcccggctga tctggtactc gtggtcgagg
     1081 atattgccct ggacctcgag gcggcccccg tcctccaggt cgacggtgaa gcggtcccgc
     1141 aggggcgtga tcatcgcctt gcggacggtg gccaccttgt cgtcgccgcg ctcgatgacc
     1201 atggtgttgc gggcccgcag cagccgcttc tgcagcttga gcagctcctg gccgtgggcg
     1261 tccttgatga ccacggtgtc gcgcacccgc agcaccttgt cgtcgaccag gaagatccgc
     1321 tcgccgcggt cgttctcgat ccagaagtcg tcgcctacag agagcagctt ctcccggagc
     1381 tggtagcggg tgccggccgg cgccccctcg ggcggctcgt cccgtcgcgc ctttcgcctg
     1441 aacatcgatc ccctcccatg gtgagccgcc accgtcccac cgggccggcc gcgcggtcat
     1501 cacccgctcg gggtgattca cccggtccgg gtgacgacgg cccacccgac ccaccggcat
     1561 cctgcgggca tggctgaggc attcggctgg ggagcgatcg gggcggccgc cctgctcgtc
     1621 ggcgccctga tcgcctacct gctggcgccg ggccggcggg tcatcgcggt ggtgatggcg
     1681 ctggggacgg gcctgctgat cggctcggtc gcctacgagc tggtcgacga cgccctggag
     1741 caccaggcgg tggtctgggt ggcggcgatg gtcctggtcg gcgccctcgt cttcaccggc
     1801 ggcgactggc tgctggaccg tggcggcggc gaggaccgca aggacgccac cggcgcccag
     1861 gccgacgggt cgccgctggc gatcgtgctc ggctcggtcc ttgacggcat ccccgagtcg
     1921 ttcgtcctcg gcctcaccgt cctccagggc ggggtcagcg tgtccctgct cgccggggtc
     1981 gccctgtcga acctgcccga gggcatgtcg tcctccagcg gcctgaaggc ggccggctgg
     2041 ccccagggcc gggtcctgac catgtggtcg gtggtggtgc tcgtctcggg cgtggccgcc
     2101 gccgccggct acgccatgct cggcccggcc agcggccgca ccggcgcgct cgtccaggcg
     2161 ttcgccggcg gggccctgct ggccatgctg gccgacaccc tgctgcccca ggcctacgcg
     2221 gtcgagggcg tgctcaccgg caccctggtc gtcatcgggt tcgcgatctc gctggccctg
     2281 tccgcggcct gagccggcga tggcgaccaa gcggtcccgg atgcgcgagc ggcgcgagca
     2341 gctggaggcg atgctggtca ccggccaggc ccggctggag cgggcccggt cgcggagcat
     2401 ggtcgtggac accatggtcg gcgtcgtccg gcgtgagcgc ccggtcgcgg tcgggatcct
     2461 ggccgcgtcc ctggccttcc gcctgttcgc catgctggtc cccctctgct acgtgctggt
     2521 ggccgggctc ggcttcgccg ccgaccgggc ggccgaggaa gccagcccgc ggggcggcaa
     2581 ccggctcggc gacctggtga tcgcctcggt ggccgccgcc acccgcacct cgggccaggg
     2641 ccgctggctg gccctgatcc tgggcgcgct ggcgacactg atcgccgccg ccgcggtcct
     2701 ggaggtgctc cgctgggtgc acctgctggc ctggcggatg gcgccggccc ggggccatgt
     2761 cagccgctgg ctggtgctcg gcctggtcac cggcctcgcc gtcgccagct tcaccgcgac
     2821 cctggccgag caggcccggg ccgacgccaa gggcctggct accgaggtga cggtg
//
LOCUS       DASSWR010000445         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_45186,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000445 DASSWR010000000
VERSION     DASSWR010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45186"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFO47_07455"
     CDS             <1..966
                     /locus_tag="VFO47_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET8788568.1"
                     /translation="KYAWLRAHHPEVADAVRWLGVAEWVVSGLGGEQVAELSLASRTG
                     LLDLERRAWWEEARAWAEAPPGLLADLVQAGSPAGRAGAGRLPRLAGATLTVAGHDHL
                     SASVGAGATRPGDLFDSCGTAEALIRAVPPPLAPDDVRRLVAAQVTVGWHVLPGRQAL
                     LGSSRAGLALQRFLGLLGVGDDQARDELDRAAAAMPSGSGGLVVRDMFGDRATLEGIG
                     ADASPALVWRAALEAVAERAAELRAHIESVAGPSRRLVLAGGWAKNPAARSVKRAVLG
                     PFDWPQVTQAGARGAALLAGVAAGRYGGIDELPGSARAAPERSLR"
     gene            963..1733
                     /locus_tag="VFO47_07460"
     CDS             963..1733
                     /locus_tag="VFO47_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788569.1"
                     /translation="MSEPLLEASGITKSFGGVRALRGVDFAVHPGEVAALVGDNGAGK
                     STLVKILSGTLAPDGGAVRFEGRQVTISNPVAARELGIETVYQDLALAPELDPAANLF
                     LGRELVRPGLLGKLGFLDKAAMRRRTNKAFADLGVGLQDTGAMVANLSGGQRQSVAVS
                     RAATWVSKVVFMDEPTAALGVVQSAHVRDLIRRVRDSGIAVVLISHNMPEVMELADRI
                     EVLRLGVRVARYRAADTTMEQVIAAMTGALGSEDGEVA"
     gene            1775..>2365
                     /locus_tag="VFO47_07465"
     CDS             1775..>2365
                     /locus_tag="VFO47_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788570.1"
                     /translation="MSETRQVPPAEVPDEVVPEPAGNGLRGVLRRRLTETTTWTFLIL
                     LGIVAGFAALRFQQFATVFNFRNIAADASGLLIIAVGMTFVIITAGIDLSVGSVLVFS
                     GVIAAKVMLAIGGGGWGAIAVGLVAGIVAGTAWGVVNGVLITKARVPPLIVTLGTLGM
                     ALGSALLITGGIDVRGVPLQLTTTIGIGQLAGVPYVV"
BASE COUNT          273 a          868 c          906 g          318 t
ORIGIN      
        1 aagtacgcct ggctacgggc ccaccacccg gaggtggccg acgccgtccg ctggctcggg
       61 gtggccgaat gggtggtcag cggcctgggt ggcgagcagg tggccgagct gtcgctcgcc
      121 tcccggaccg gcctcctcga cctggaacgg cgggcctggt gggaggaggc ccgggcgtgg
      181 gccgaggcgc cgccggggct gctggccgac ctggtccagg cgggcagccc ggccggccgg
      241 gccggcgccg gacggctccc ccgcttggcc ggggcgaccc tcaccgtggc cggccacgac
      301 cacctcagcg cctcggtcgg ggccggcgcg acccggcccg gcgacctgtt cgactcctgc
      361 ggcaccgccg aggcgctcat ccgggccgtc cctcccccgc tcgcgcccga cgacgtccgc
      421 cggctggtcg ccgcccaggt gaccgtcggc tggcacgtcc tgcccggccg ccaggccctg
      481 ctcgggtcct ccagggccgg gctcgccctg cagcggttcc tcggtctgct cggggtcggc
      541 gacgaccagg cccgcgacga gctcgaccgg gccgcggcgg ccatgccgtc cggcagcggc
      601 ggcctggtcg tgcgggacat gttcggcgac cgggcgacgc tcgaggggat cggcgccgat
      661 gccagccccg cgctcgtgtg gcgggcggcg ctggaggcgg tggccgagcg ggcggccgag
      721 ctgcgcgccc acatcgagtc ggttgccggg ccgagccggc ggctggtgct ggccggcggc
      781 tgggcgaaga acccggccgc ccgctcggtg aagcgggcgg tgctcggccc gttcgactgg
      841 ccgcaggtca cccaggccgg cgcccgcggg gccgcgctcc tggccggcgt cgccgccggc
      901 cgctacggag ggatcgacga gctgccgggg tcggcccgtg cggcgccaga gaggagcctg
      961 cggtgagcga acccctgctg gaggcgagcg gcatcacgaa gagcttcggc ggcgtccggg
     1021 cgctgcgggg cgtcgacttc gccgtccacc cgggcgaggt ggcggccctc gtcggcgaca
     1081 acggcgccgg caagagcacg ctggtcaaga tcctgtcggg caccctcgcc cccgacggag
     1141 gcgcggtccg gttcgagggc cgccaggtca ccatctcgaa cccagtcgcg gcccgcgagc
     1201 tcggtatcga gaccgtctac caggacctgg ccctggcgcc cgagctcgac ccggcggcca
     1261 acctgttcct cggccgcgaa ctggtccgcc ccgggctgct cggcaagctc gggttcctgg
     1321 acaaggcggc catgcggcgc cggaccaaca aggccttcgc cgacctcggc gtcgggctcc
     1381 aggacaccgg ggccatggtg gccaacctgt cgggtgggca gcgccagagc gtcgccgtca
     1441 gccgggccgc cacctgggtc agcaaggtcg tgttcatgga cgagcccacc gccgccctcg
     1501 gcgttgtcca gtcggcgcat gtgcgcgacc tgatccggcg ggtgcgcgac tcgggcatcg
     1561 cggtcgtgct gatcagccac aacatgccag aggtgatgga gctggccgac cggatcgagg
     1621 tgctccggct cggggtccgg gtggcccgct accgcgccgc cgacaccacc atggagcagg
     1681 tcatcgccgc catgaccggc gccctcggca gcgaggacgg agaggtggcc tagatgcgcc
     1741 ggatgggttt ggggaacccc gagggggtgc cccaatgagc gagacccggc aggtgccgcc
     1801 ggccgaggtg ccggacgagg tggtgccgga gccggcgggc aacggcctgc ggggcgtgct
     1861 ccgccgccgg ctgaccgaga ccaccacctg gacgttcctg atcctgctcg gcatcgtggc
     1921 cggcttcgcc gccctgcgct tccagcagtt cgccacggtg ttcaacttcc gcaacatcgc
     1981 cgccgacgcc tcggggctgc tgatcatcgc cgtcggcatg accttcgtga tcatcaccgc
     2041 cgggatcgac ctctcggtcg ggtcggtgct ggtcttctcc ggggtgatcg cggccaaggt
     2101 gatgctggcc atcggcggcg ggggctgggg cgcgatcgcg gtcgggctgg tcgccgggat
     2161 cgtcgccggc accgcctggg gcgtggtcaa cggcgtgctc atcaccaagg ccagggtgcc
     2221 gccgctgatc gtcaccttgg gaacccttgg gatggcgctc gggtcggccc tgctgatcac
     2281 cggcgggatc gacgtgcgcg gcgtccccct ccagctgacc accaccatcg ggatcgggca
     2341 gcttgccggc gtcccctacg tcgtc
//
LOCUS       DASSWR010000446         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_412981,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000446 DASSWR010000000
VERSION     DASSWR010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412981"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..374)
                     /locus_tag="VFO47_07470"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="VFO47_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837543.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
     gene            complement(375..1817)
                     /gene="purF"
                     /locus_tag="VFO47_07475"
     CDS             complement(375..1817)
                     /gene="purF"
                     /locus_tag="VFO47_07475"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET8788571.1"
                     /translation="MLDADHPREACGVFGVWAPGEQVANLVFYGLYALQHRGQESAGM
                     AVADGRGVLVYKEMGLVSQVFDEPTLATLQGHLAIGHTSYSTTGGSRWENAQPAFKTN
                     AAGGGIALAHNGNLTNTAVLAEPLGLGAPDAAGGERRPSSDTDVMAELLAREADLSLE
                     EAIVRTMPRLEGAFSLVIMDERTLYGVRDRHGVRPLCIGKLPGGWVIASETAALDIVG
                     ATLVREVEPGEVVAIDGQGLRARRFAPLDPRLCVFEHVYLARADSTLAGQSVHQVRRA
                     LGAALAVEAPVEADLVIPVPDTAWSAAAGYAEASGIPFGEGLMKNRYVGRTFIQPSQT
                     MRQLGIRIKLNPLKETIRGLRLVVVDDSIVRGNTTKAIVTMLREAGAREVHVRITSPP
                     VRWPCFYGIDMPTRAELVASDLLTDEVASYVGADSLGYLSLEALTETAADPGTGPGGL
                     CTACFTGRYPIPVEAQVQGKELLEGLAPQR"
     gene            2215..>3712
                     /locus_tag="VFO47_07480"
     CDS             2215..>3712
                     /locus_tag="VFO47_07480"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET8788572.1"
                     /translation="MIRNMQIRSKLIAVLVIPLVALTVLAALAIGAAVSRGVQADRVN
                     DQTAFAISLSNLVHQLQRERDLSAAWVGSGRDAGYGGVVAQRVAVNQALQTFRQDVED
                     LGNEDSAFRERVDAAVAELGELNAQRELIANDEDMSIERTLDYYSSVIDELLAVNLEI
                     AAQTDDRDLIRNVGTFVSLARLKEATSLERGRLYAVASVGKFGPGDFRELAPIVGAQE
                     AWRSQFDATATPEQRAFLDRTLQGPDITRVDELRTKVLTGDPSQPVDIDPEQWFTYMS
                     AKLDLLRTVEGQLADDVLAASEAAQSAASRQALLYTIILTLVLWVTIGLSLWLARSMV
                     GPLRSLTRSANEVADERLPSLVDRLHHTNDPRDHDVVPEPVPVDANDEIGQVSAAFNS
                     VHRVAIQVATEQAALRKSIGDMFLNLARRSQSLIDRQLELIDDLERSEADPDSLENLF
                     KLDHLATRMRRNAEDLIVLSGAEPARRWSQPVTLVDVVRAALAEVEDYN"
BASE COUNT          538 a         1373 c         1298 g          503 t
ORIGIN      
        1 ctccgggtcg acccggccca cggccaggta gtcgaggacg aacagcggct cggcccggtc
       61 acgaccacgt catcgaccac catggcgacc aggtcgatac cgaccgtgtc gtggcggtcg
      121 agggcctggg cgaccagcag cttggtgccg acgccgtcgc tggccgcggc caggaccggg
      181 tcgccgggca ggccggccgc gccggccccg ccggaggccc cggccagccg ccacagcccg
      241 gcgaacccgc cgatgccgcc cagcaccctg ggcccgtggg tcgcctccac cgcggcccgc
      301 atcagggcca cggcgcggtc accggcctcg acgtcgaccc cggcctgggc gtaggtgagc
      361 ggttccctct ccatctagcg ctggggcgcc aggccctcga gcagctcctt gccctggacc
      421 tgggcctcca ccgggatcgg gtacctcccc gtgaagcagg ccgtgcacag gccccccggc
      481 cccgtcccgg ggtcggccgc ggtctcggtc agggcctcca gcgacaggta gccgagcgag
      541 tcggcgccga cgtagctggc cacctcgtcg gtgagcaggt cgctggccac cagctcggcc
      601 cgggtcggca tgtcgatgcc gtagaagcag ggccagcgca ccgggggcga ggtgatgcgg
      661 acgtggacct cgcgggcccc ggcctcgcgg agcatggtga cgatcgcctt ggtggtgttg
      721 ccgcgcacga tcgagtcgtc gaccaccacc agccgcagcc cccggatcgt ctccttgagc
      781 gggttgagct tgatgcggat gccgagctgg cgcatggtct ggctcggctg gatgaaggtg
      841 cggccgacgt agcggttctt catcaggccc tcgccgaagg ggatgccgct ggcctcagcg
      901 tagccggcgg cggcgctcca ggcggtgtcg ggcacgggga tgaccaggtc ggcctcgacc
      961 ggcgcctcca cggccagggc ggccccgagg gcgcggcgga cctggtggac gctctggccg
     1021 gccagggtgg agtcggcccg ggccaggtag acgtgctcga acacgcacag ccgcgggtcg
     1081 agcggggcga agcggcgggc ccgcaacccc tgcccgtcga tggccacgac ctcgccgggc
     1141 tcgacctcgc ggaccagggt ggccccgacg atgtccaggg ccgccgtctc cgacgcgatc
     1201 acccagcctc ccggcagctt gccgatgcac aggggccgca ccccgtggcg gtcgcgcacc
     1261 ccgtagaggg tccgctcgtc catgatgacc agcgagaacg ccccctcgag gcggggcatg
     1321 gtacggacga tcgcctcctc cagcgacagg tcggcctcgc gggccagcag ctcggccatc
     1381 acgtcggtgt cgctgctcgg ccgccgctcg ccgcctgccg cgtccggcgc cccgaggccc
     1441 agcggctcgg ccagcacggc cgtgttggtg aggttgccgt tgtgggccag ggcgatcccg
     1501 cccccggcgg cgttggtctt gaacgccggc tgggcgttct cccagcggga gccgccggtg
     1561 gtcgagtagc tggtgtggcc gatcgccagg tgcccctgga gggtggccag tgtcggctcg
     1621 tcgaagacct ggctgaccag gcccatctcc ttgtagacga gcacgccgcg gccgtcggcg
     1681 acggccatcc cggccgattc ctggcctcgg tgctggaggg cgtagagccc gtagaagacg
     1741 aggttggcga cctgctcccc cggagcccag actccgaaga ccccgcacgc ctcgcgtgga
     1801 tggtcggcgt cgagcatgtt cctcctggcc gtgaaggccg agcgggcggt cggggaagcc
     1861 atccgatcgt agcaccggac cctgacgaac cggtggatgc ggagatgcac cccttgcccg
     1921 gcttctcgcg tcggtggtaa gaatcgcggg ttgtccaggg acacccctcc aacgcctgta
     1981 tgtgctgatg aacaggggtg tacgggggaa gcggatgttt gcaaagccaa gcgcgcaaca
     2041 ggtagattcc cgtctgatcc ccctgtatga ccgagccagc ccgtcccttc cccggggccg
     2101 ttctatgagc aggaatgccg tgcgccttcg ggcacgcaga ccgccggccg ccgcggtgac
     2161 gagcacccat ctgcctcgag tccagctccc aggcaccatc gcagaggtca gcccatgatc
     2221 cgaaacatgc agatccggtc caagctgatc gcggtgctgg tcatcccgct ggtcgccctg
     2281 acggtgctgg ccgcgctcgc catcggcgcc gccgtcagcc gcggcgtcca ggccgaccgg
     2341 gtcaacgacc agacggcctt tgccatcagc ctgtcgaacc tggtgcacca gctccagcgc
     2401 gagcgcgacc tgtcggccgc ctgggtcggg agcggccgcg acgccggcta cggcggcgtg
     2461 gtcgcccagc gggtggcggt caaccaggcg ctgcagacct tccggcagga cgtggaggac
     2521 ctcggcaacg aggactcggc gttccgggag cgggtcgacg ccgccgtcgc cgagctcggg
     2581 gagctcaacg cccagcgcga gctgatcgcc aacgacgagg acatgagcat cgagcggacg
     2641 ctcgactact actcgagcgt catcgacgag ctgctcgccg tcaacctcga gatcgccgcc
     2701 cagaccgacg accgggacct gatccgcaac gtcggcacct tcgtctcgct ggcccgcctc
     2761 aaggaggcga ccagcctgga gcggggccgc ctgtacgcgg tcgcctcggt cgggaagttc
     2821 ggcccgggcg acttccggga gctggccccg atcgtcggcg cccaggaggc ctggcggtcc
     2881 cagttcgacg ccacggcaac gcccgagcag cgcgccttcc tcgaccgcac cctgcagggc
     2941 cccgacatca cccgggtcga cgagctccgt accaaggtcc tgaccggcga ccccagccag
     3001 ccggtcgaca tcgaccccga gcagtggttc acctacatgt cggccaagct cgacctgctg
     3061 cgcacggtcg agggccagct ggccgacgac gtcctcgcgg ccagcgaggc cgcccagtcc
     3121 gcggccagcc gccaggccct gctctacacg atcatcctga ccctggtgct gtgggtcacc
     3181 atcggcctgt ccctgtggct ggcccgctcc atggtcgggc cgctgcggag cctgacccgc
     3241 agcgccaacg aggtcgccga cgagcgcctg cccagcctgg tcgaccggct gcaccacacc
     3301 aacgaccccc gcgaccacga cgtggtcccg gaaccggtgc cggtggacgc caacgacgag
     3361 atcggccagg tgtcggccgc cttcaactcg gtgcaccggg tggccatcca ggtcgccacc
     3421 gagcaggccg ccctgcgcaa gtcgatcggc gacatgttcc tcaacctggc ccggcgcagc
     3481 cagagcctga tcgaccgcca gctggagctg atcgacgacc tggagcgcag cgaggccgac
     3541 cccgactccc tggagaacct cttcaagctc gaccacctgg cgacccggat gcggcgcaac
     3601 gccgaggacc tgatcgtgct ctcgggggcc gagccggccc ggcgctggag ccagccggtg
     3661 accctggtcg acgtggtccg ggccgccctg gccgaggtcg aggactacaa cc
//
LOCUS       DASSWR010000447         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1146844, whole genome shotgun sequence.
ACCESSION   DASSWR010000447 DASSWR010000000
VERSION     DASSWR010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146844"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFO47_07485"
     CDS             complement(<1..462)
                     /locus_tag="VFO47_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8788573.1"
                     /translation="MAPAADVVVVGAGLAGLAAATRLVDAGAEVVVLEARDRVGGRTL
                     TLPAADGTPIDHGGQWVGPPHDRIATLAERVGVTTYPTWERGLNTVVRDGQAHRYEGD
                     PLDEGDPISAVALGQAIRELDAMAGTVPPEAPWTAADALAWDSQTVETWLQT"
     gene            681..>2077
                     /locus_tag="VFO47_07490"
     CDS             681..>2077
                     /locus_tag="VFO47_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8788574.1"
                     /translation="MAIIEDRTTRVRTFDSTSPATGEVVGSFPMDGPEQVAAAVARAR
                     EAQRGWEELGFDGRRRALLAYKALLVRRTDELVALVHRENGKPHADALIEVLMIVEHL
                     DWAARNAARVLGPRRVRSTLVLANHAASVEYQPLGVVGVIGPWNFPVFTPLGSVVYAL
                     AAGNAVVLKPSELTPAVGCFLADTFAEAVPGQPVFQVVTGDGATGHALCLAGVDKVAL
                     TGSPATGRKVMAACAERLTPVVLELGGKDAMVVDADADLDAAADQAVWGGMANAGQTC
                     LATERVYVVDAVYEPFLEKVVEKAARIRPGDGPDADFGPITLPRQLEVIRRHLDDAFA
                     RGARALVGGPDAVRPPYVDPVVLVDVPADALVMREETFGPVLPVTRARDAGHAVALAN
                     ASPYGLGAAVFAGRGAGRIARALRAGSVSVNSVLTFAGMPSLPMGGVGESGFGRVHGP
                     DGLRELSRAKAVSRQR"
BASE COUNT          238 a          783 c          786 g          270 t
ORIGIN      
        1 ggtctggagc caggtctcta ccgtctggga gtcccaggcg agcgcgtcgg cggccgtcca
       61 gggcgcctcc ggcgggaccg tgcccgccat cgcgtccagc tcgcggatgg cctgcccgag
      121 ggcgacggcg cttatcgggt cgccctcgtc gagcgggtcg ccctcgtagc ggtgggcctg
      181 gccgtcgcgg accacggtgt tgagcccccg ctcccaggtc gggtaggtgg tgacgccgac
      241 ccgctcggcc agggtggcga tgcggtcgtg gggcggcccg acccactggc cgccgtggtc
      301 gatcggggtg ccgtcggcgg cgggcagggt cagggtccgc ccgccgaccc ggtcgcgcgc
      361 ctccagcacc accacctcgg cgccggcgtc caccagccgg gtcgcggcgg ccaggccggc
      421 cagcccggca ccgaccacga cgacgtctgc tgcgggggcc actgctctct ctcctcgctc
      481 ggatggcgtc ctgggggcct gcgatgccag ggatgctgcg gcggtcgggc gagtcacggc
      541 ccctcgcgag cgccgagcgg gatgcgggcg aatgctacga aggtgcgacg ggaaaacacc
      601 agcgccgtgt ccgcttcgcg acgatctggt gccgccccgc ccgccgaatg ggtacattcc
      661 ttttcgagaa aggggacggc atggcgatca tcgaggaccg cacgacccgc gtccgcacct
      721 tcgactcgac cagcccggcc accggggagg tggtggggag cttcccgatg gacgggcccg
      781 agcaggtcgc ggccgccgtg gcccgggccc gggaggccca gcggggctgg gaggagctgg
      841 gcttcgacgg ccgccggcgg gccctgctgg cctacaaggc gctgctggtc cgccgcaccg
      901 acgagctggt cgccctggtc caccgggaga acggcaagcc gcacgccgac gccctgatcg
      961 aggtgctgat gatcgtcgag cacctcgact gggcggcccg caacgccgca cgggtgctgg
     1021 ggccgcgccg ggtccggagc accctggtgc tggccaacca cgccgcctcg gtcgagtacc
     1081 agccgctggg ggtggtgggc gtcatcgggc cctggaactt cccggtgttc acccccctgg
     1141 ggtcggtcgt ctacgccctg gccgccggca acgccgttgt cctcaagccg agcgagctga
     1201 ccccggcggt gggctgcttc ctggccgaca ccttcgccga ggccgttccc ggccagccgg
     1261 tgttccaggt ggtgacgggg gacggggcca ccggccacgc cctctgcctg gccggggtgg
     1321 acaaggtcgc cctcaccggg tcgccggcca ccggccgcaa ggtgatggcc gcctgcgccg
     1381 agcgcctcac cccggtggtg ctggagctcg gcggcaagga cgccatggtg gtggacgccg
     1441 acgccgacct ggacgccgcc gccgaccagg cggtctgggg cggcatggcc aacgccggcc
     1501 agacctgcct ggccaccgag cgcgtctacg tggtcgacgc cgtctacgag ccgttcctgg
     1561 agaaggtggt cgagaaggca gcgcggatcc ggcccgggga cggccccgac gccgacttcg
     1621 gccccatcac cctgccccgg cagctggagg tcatccggcg gcaccttgac gacgccttcg
     1681 cccgcggggc cagggccctg gtcggcggcc cggacgcggt ccgcccgccc tacgtcgacc
     1741 cggtggtgct ggtcgacgtg cccgccgacg ccctggtcat gcgggaggag accttcggcc
     1801 cggtcctgcc cgtgaccagg gcccgcgacg ccggccacgc cgtcgccctg gccaacgcca
     1861 gcccctacgg cctcggcgcg gccgtgttcg ccggccgcgg cgccggccgc atcgcccggg
     1921 ccctgcgggc gggcagcgtc agcgtcaact cggtgctgac cttcgccggc atgccgtcgc
     1981 tgcccatggg cggggtgggg gagtccggct tcggccgcgt ccacggcccc gacggcctgc
     2041 gcgagctctc ccgggccaag gccgtcagcc gccagcg
//
LOCUS       DASSWR010000448         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_695465,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000448 DASSWR010000000
VERSION     DASSWR010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695465"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFO47_07495"
     CDS             complement(<1..552)
                     /locus_tag="VFO47_07495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET8788575.1"
                     /translation="MGLVLAVLVTGLLIDQAAPGGRVAVGLGLAACLLAVARAARLTV
                     ADLGLSRPAWPSGLRWGAAAAAVVAAAYALALLVPPVRQALPDTGGGIGRAALLAALV
                     VVPLGTVLPEELAFRGLLLALLGRGHGVRAATLLSSGLFGLWHILPSLGGGAANAAIT
                     GAVGGDAAGTLLRVAVTVCFTFAA"
     gene            687..1169
                     /locus_tag="VFO47_07500"
     CDS             687..1169
                     /locus_tag="VFO47_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET8788576.1"
                     /translation="MAHLVVLGVDNRDDAERLFDLAGDLAKQELLQLQDAAYAYRDDK
                     GKVRIHQAVSLTGAGAASGALWGTLIGLIFLMPVAGLAIGAGTGALAGKLADVGINDD
                     TIKQIGSQLEGGRAAVFLLAKSATVDRVIEAIKPFNPTVIQTNLSHDREEELVKALQS
                     "
     gene            1270..1395
                     /locus_tag="VFO47_07505"
     CDS             1270..1395
                     /locus_tag="VFO47_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788577.1"
                     /translation="MSATGLGVMGATLADGGGIVVVSPGKGALASGRPGEQEEQR"
     gene            1392..>2233
                     /locus_tag="VFO47_07510"
     CDS             1392..>2233
                     /locus_tag="VFO47_07510"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET8788578.1"
                     /translation="MTERAAGGAGVVAVAGEALVDFVPAGGPEVFQAAPGGSPANVAV
                     GLARQEVPTRLLARIADDLLGHRLRAHLDGNGVDLSYAVRAAEPTSLAIVAVGRDGVV
                     EYDFRVEGTADWQWRDDELAGALDGGVVALHAGSIALTMPPGADVLQRLLARARQSMT
                     VTYDPNCRPLLMGSPDAVRGRIESLVALADVVKASADDLDWLLPGRAPEQVAEAWLAK
                     GPSMVVITLGPAGLLAATRQAGVLGRPGRAVEVVDTVGAGDACMAALLAGLHRRDLLG
                     GVRR"
BASE COUNT          312 a          820 c          863 g          238 t
ORIGIN      
        1 ggcggcgaag gtgaagcaga cggtgacggc gacgcggagc agcgtcccgg cggcgtcacc
       61 gcccaccgcg ccggtgatgg cggcgttcgc ggcgccgccg ccgagcgacg gcaggatgtg
      121 ccagaggccg aacaggcccg aagacaacag ggtcgcggcc cgcacgccgt ggccgcgccc
      181 cagcagggcc agcagcaggc cccggaaggc gagctcctcg ggcaggacgg tccccagcgg
      241 gaccaccacg agcgcggcca gcagcgccgc ccgcccgatg ccgccgccgg tgtccgggag
      301 ggcctggcgc acgggcggga ccagcagggc cagggcgtag gcggcggcga cgaccgccgc
      361 cgcggccgcg ccccagcgca ggcccgacgg ccaggccggc cgggacagcc cgaggtcggc
      421 cacggtcagc cgggcggccc gggccaccgc gagcaggcac gcggccagcc ccagcccaac
      481 ggccacgcgg ccaccggggg ccgcctggtc gatcagcagg cccgtgacca gcacggcgag
      541 gacaagcccc accgccagcg tgctagggtt gcgctcgagc aaggcaagct ccacggggaa
      601 tcacccgcaa agggtgaaga cggctcgacc cgtcaggctg aatcatgacc ggcgggaagc
      661 aacgttcgct cacgtacgga ggaaccatgg cccatctggt ggtgctcggc gtcgacaacc
      721 gcgacgacgc cgagcggctc ttcgacctgg ccggcgacct ggccaagcag gagctgctgc
      781 agctccagga cgccgcctac gcctacaggg acgacaaggg caaggtccgc atccatcagg
      841 cggtcagcct gaccggggcc ggcgcggcct cgggcgccct ctggggcacg ctcatcgggc
      901 tcatcttcct gatgccggtg gccggcctgg ccatcggggc cggcaccggc gccctggccg
      961 gcaagctggc cgacgtcggc atcaacgacg acaccatcaa gcagatcggc agccagctcg
     1021 agggcggcag ggcggccgtg ttcctgctcg ccaagtcggc caccgtcgac cgggtgatcg
     1081 aggccatcaa gcccttcaac cccacggtca tccagaccaa cctgagccac gaccgcgagg
     1141 aggagctcgt caaggcgctc cagtcctgac ggttccgatg gaggatgccc cgttcgtctc
     1201 gacggggcat cctccgtctc cggagcaggt gcggcgctcg gtcgacgatg agggcagagc
     1261 tccctgcggg tgagcgcaac cgggctgggg gtgatgggcg ccaccctggc cgacggcggc
     1321 gggatcgtgg tggtctcccc gggcaagggg gcgctggcca gtggccgccc aggcgagcag
     1381 gaggagcagc gatgaccgag cgggcggccg gcggagccgg ggtggtggcg gtggccgggg
     1441 aggcgctggt cgacttcgtg cccgccggcg ggcccgaggt gttccaggcc gcgccgggcg
     1501 ggagcccggc gaacgtggcc gtggggctgg cccgccagga ggtgccgacc cggctgctgg
     1561 cccggatcgc cgacgacctg ctcgggcacc ggctgcgggc ccacctggac ggcaacgggg
     1621 tcgacctgtc gtacgccgtg cgggcggccg agccgacctc gctggccatc gtggccgttg
     1681 ggcgcgacgg ggtggtcgag tacgacttcc gcgtcgaggg cacggccgac tggcagtggc
     1741 gcgacgacga gctcgccggg gccctcgacg gcggggtcgt ggccctgcac gccgggtcga
     1801 tcgcgctcac catgccgccc ggagccgacg tgctccagcg gctgctggcc cgggcccgcc
     1861 agtccatgac cgtgacctac gaccccaact gccggcctct gctgatgggc tcccccgacg
     1921 ccgtccgcgg ccgcatcgag tccctggtcg ccctggccga cgtggtcaag gccagcgccg
     1981 acgacctgga ctggctgctc cccggacggg cccccgagca ggtggccgag gcctggctgg
     2041 ccaagggccc ctcgatggtg gtgatcaccc tcggcccggc cgggctgctg gccgcgaccc
     2101 gccaggcggg cgtgctgggc cgccccggcc gggcggtcga ggtggtcgac accgtcggcg
     2161 ccggcgacgc ctgcatggcc gccctgctgg ccgggctgca ccgccgcgac ctgctcggcg
     2221 gcgtccggcg gga
//
LOCUS       DASSWR010000449         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1232842, whole genome shotgun sequence.
ACCESSION   DASSWR010000449 DASSWR010000000
VERSION     DASSWR010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232842"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFO47_07515"
     CDS             complement(<1..190)
                     /locus_tag="VFO47_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET8788579.1"
                     /translation="MIAKLLAANRGEIATRVLRTARAMGIATVAVFSDPDRDAPFGAM
                     ADEAVPLGGAAPADSYLRG"
     gene            complement(187..1785)
                     /locus_tag="VFO47_07520"
     CDS             complement(187..1785)
                     /locus_tag="VFO47_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET8788580.1"
                     /translation="MGVLGSRLDPSSDAYRANREANQELLAELDRLLAQARAGGGPKY
                     VERHRARGKLLARERVELLVDRDGPFLELSPLAAWGTDFPVGASGVTGVGPVAGVECV
                     ITASDPTVRGGATNPFGLRKTLRAMEIAERNRLPLVQLIESSGADLPTQSQIFIPGGA
                     VFRNLTRLSRAGIPTLALVFGSSTAGGAYVPGLSDHTIMVRERAKVFLGGPPLVRMAT
                     GEQATDEELGGAEMHARISGLADQLAADERDCIRLGRRAIADLGWRKLGPGPTRPADE
                     PVHDPDELLGLAPVDLKVPADVREILARVVDGSRFSEWKPLYGTSLVCGWASVHGFPV
                     GVLANAAGVLFSEESNKAAHFIQLANQADTPLLFLQNTTGYMVGKAYEQRGIIKDGAK
                     MINAVANSTVPHVTLILGASYGAGGYGMAGRAYDPRFLFTWPASRSAVMGPEQLAGVM
                     SILNRASAERAGRPFDDEADRSMREAVRDHVEAESRALFNTGLLYDDGIIDPRDTRTV
                     LGMALSACHSGEVKGTDGFGVFRM"
     gene            complement(1894..>2409)
                     /locus_tag="VFO47_07525"
     CDS             complement(1894..>2409)
                     /locus_tag="VFO47_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8788581.1"
                     /translation="QADGAKECAVELYSMTKAFSMAGWRMAYLVGKAEVVQALAKLKS
                     YLDYGAFQPIQIAATVTLNEDPDHPERAQAIYQSRRDALCDGLARIGWEVEPPRGTMF
                     VWAPIPEPYRELGSVEFASMLVKEAKVATSPGIGFGPGGDGHVRFALIENEQRIGQGI
                     RNLRRALTKLG"
BASE COUNT          316 a          861 c          906 g          326 t
ORIGIN      
        1 cgccgcggag gtaggagtcg gccggcgcgg ccccgcccag gggcaccgcc tcgtcggcca
       61 tggccccgaa gggggcgtcg cggtcggggt cggagaacac ggccacggtg gcgatgccca
      121 tggcccgggc ggtgcggagc acccgggtgg cgatctcgcc ccggttggcg gccagcagct
      181 tggcgatcac atccggaaca ccccgaagcc gtccgtgccc ttcacctccc ccgagtggca
      241 ggccgacagc gccatgccga gcacggtgcg ggtgtcgcgc gggtcgatga tgccgtcgtc
      301 gtacagcagg ccggtgttga acagggcccg ggactcggcc tcgacgtggt cgcggacggc
      361 ctcgcgcatg gaccggtcgg cctcgtcgtc gaacggccgc ccggcccgct cggccgaggc
      421 ccggttgagg atcgacatga ccccggcgag ctgctcgggc cccatcaccg ccgacctgga
      481 cgccggccag gtgaacagga accgggggtc gtaggcccgg ccggccatgc cgtagccgcc
      541 ggcgccgtag gacgcgccga ggatcagggt gacgtgcggc accgtcgagt tggccacggc
      601 gttgatcatc ttggccccgt ccttgatgat cccccgctgc tcgtacgcct tgccgaccat
      661 gtagccggtg gtgttctgga ggaacagcag cggcgtgtcg gcctggttgg ccagctggat
      721 gaagtgggcc gccttgttcg actcctcgga gaacagcacc ccggccgcgt tcgccagcac
      781 cccgaccgga aagccgtgga ccgacgccca gccgcagacc aggctggtgc cgtacagcgg
      841 cttccactcc gagaaccggg agccgtcgac caccctggcc aggatctcgc gcacgtcggc
      901 ggggaccttc aggtccaccg gggccaggcc gagcagctcg tcggggtcgt gcacgggctc
      961 gtcggccggc cgggtcggcc cggggccgag cttgcgccag ccgaggtcgg cgatcgcccg
     1021 gcggccgagg cggatgcagt cgcgctcgtc ggcggccagc tggtcggcca gcccggagat
     1081 gcgggcgtgc atctcggccc cgcccagctc ctcgtcggtg gcctgctcgc cggtggccat
     1141 ccggaccagt ggggggccgc ccaggaacac cttggcccgc tcccggacca tgatggtgtg
     1201 gtcggacagc cccgggacgt aggcgccccc ggcggtggac gacccgaaga ccagggccag
     1261 ggtggggatg ccggcccggg acagccgggt caggttgcgg aacacggccc cgccggggat
     1321 gaagatctgc gactgggtgg gcaggtcggc cccgctcgac tcgatcagct ggaccagggg
     1381 cagccggttg cgctcggcga tctccatcgc ccgcagggtc ttgcggagcc cgaacgggtt
     1441 ggtggccccg ccgcggacgg tcgggtcgct ggccgtgatg acgcactcga ccccggcgac
     1501 gggcccgacg ccggtgacgc cgctggcccc caccgggaag tcggtccccc aggcggccag
     1561 cggcgacagc tccaggaacg gcccgtcccg gtcgaccagc agctcgaccc gctcccgggc
     1621 cagcagcttg ccccgggccc ggtggcgctc gacgtacttg ggcccgccgc ccgccctggc
     1681 ctgggccagc agccggtcca gctcggccag cagctcctgg ttggcctcgc ggttggcccg
     1741 gtaggcgtca gaggacgggt cgagccggct cccgagcacg cccatcctta cccccagccg
     1801 ttgtggttac cgagaggtaa ccacgggccg ggcccgggcg ctagaccgcg ctcagggctc
     1861 gtcgcggggg tcgagcgact tgacccggtc ggctcagccg agcttggtca gggcgcggcg
     1921 caggttgcgg atcccctggc cgatgcgctg ctcgttctcg atcagggcga agcggacgtg
     1981 gccgtcgccg cccgggccga agccgatgcc cggggaggtg gccaccttgg cctccttgac
     2041 cagcatggag gcgaactcga ccgagcccag ctcccggtag ggctcgggga tcggcgccca
     2101 gacgaacatg gtcccccggg gcggctcgac ctcccagccg atccgggcca ggccgtcgca
     2161 gagggcgtcg cggcgggact ggtagatcgc ctgggcccgc tcggggtggt cggggtcctc
     2221 gttcagggtc acggtggcgg cgatctggat cggctggaag gcgccgtagt cgaggtagga
     2281 cttgagcttg gccagggcct gcaccacctc ggccttgccg accaggtagg ccatgcgcca
     2341 gccggccatg gagaaggcct tggtcatcga gtacagctcg accgcgcact ccttggctcc
     2401 gtcggcctg
//
LOCUS       DASSWR010000450         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_722495,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000450 DASSWR010000000
VERSION     DASSWR010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722495"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFO47_07530"
     CDS             <1..586
                     /locus_tag="VFO47_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788582.1"
                     /translation="QVADAEQVRAAFDRWDRELAPGATGWLGSTAGVTDDGRFIALAR
                     FESEEAARRNSGRPEQDRWWAETARLFSGEASFTDSDDVVVDVVGEPGDAGFVQVMRG
                     RGTDPDRARELMAQDSSEWADFRPDILGSVAVLHDGGAYTMAIYFTSEEEAREGERKE
                     PPPELKAQMEEMDAISDGVPEFFDLRQPWLYSPR"
     gene            complement(590..1837)
                     /locus_tag="VFO47_07535"
     CDS             complement(590..1837)
                     /locus_tag="VFO47_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8788583.1"
                     /translation="MPPVTLAVVGAGNRGTTHGDWALANPDRARVVAVAEPRQARRVR
                     FAAGHGLPAGAVFADWRELAGRGRTADAVLVCTQDAMHVEPAIAFAGLGYHVLLEKPM
                     ATTEAGCRQIVEAAERAGVILAVCHVLRYTPYTALVKQVVDAGRLGQVLSVQHLEPVG
                     FLHQAHSYVRGPWRRQDAATFMLMAKSCHDLDWLQYVVGRRIRRVASFGGLSHFRPEH
                     RPAGAADRCLDCVVEPTCPYSAVRFYLSRLESGPGWPLDAVVDRYTRADLLAALRHGP
                     YGRCVWACDNDVVDHQVVAMEFDGGPTGTFTMTGFNAGGHRRTRLFGTRGELDGDGET
                     VRVHDFLTQTTEVLSARPPGDATAGGGHGGGDWGLMDAFVRAVATGDRAHVLTGPRES
                     LAAHLAVFAAERARQEGVVVTVA"
     gene            complement(1846..>2230)
                     /locus_tag="VFO47_07540"
     CDS             complement(1846..>2230)
                     /locus_tag="VFO47_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET8788584.1"
                     /translation="DVLVPNRVELAQLAGGAVPATVAEAVELAGRLPARAVVVTLGAD
                     GALVVEDGDAGHVPAVPVRAVDTTAAGDAFCGGLADALAAGAALEDAARRAARVAAAA
                     CLRPGAQASLPTPADLRALPEGAGA"
BASE COUNT          291 a          819 c          883 g          237 t
ORIGIN      
        1 gcaggtggcc gacgcggagc aggtccgggc ggcgttcgac cggtgggacc gggagctggc
       61 ccccggcgcc accggctggc tcggctcgac cgccggcgtg accgacgacg gccgcttcat
      121 cgccctggcc cgcttcgagt ccgaggaggc ggcccggcgc aacagcggcc ggcccgagca
      181 ggaccgctgg tgggccgaga ccgcccggct gttcagcggc gaggccagct tcaccgacag
      241 cgacgacgtg gtcgtggacg tcgtcggcga gcccggcgac gccggcttcg tccaggtcat
      301 gcggggccgc ggcaccgacc ccgaccgcgc ccgggagctg atggcccagg actcgtccga
      361 gtgggccgac ttccgtcccg acatcctcgg cagcgtcgcc gtcctccacg acggtggggc
      421 ctacaccatg gccatctact tcacctccga ggaggaggcc cgcgagggcg agcgcaagga
      481 gccgcccccc gagctcaagg cgcagatgga ggagatggac gccatcagcg acggcgtgcc
      541 cgagttcttc gacctcaggc agccctggct gtactcgccg cgctgacggt caggcgacgg
      601 tcacgaccac gccctcctga cgggcccgtt cggcggcgaa cacggccagg tgggcggcca
      661 gcgactcccg ggggccggtg aggacgtggg cgcggtcccc ggtggccacg gcgcggacaa
      721 aggcgtccat cagcccccag tcgccgccgc cgtggccgcc gccggcggtg gcgtcccccg
      781 ggggccgggc ggacaggacc tcggtcgtct gggtgaggaa gtcatggacc cggacagtct
      841 cgccgtcgcc gtcgagctcg ccgcgggtgc cgaacaggcg ggtccggcgg tggccgccgg
      901 cgttgaagcc ggtcatggtg aacgtgcccg tggggccgcc gtcgaactcc atggccacga
      961 cctggtgatc gaccacgtcg ttgtcgcacg cccagacgca gcgcccgtag gggccgtggc
     1021 gcagggcggc caggaggtcg gcccgggtgt agcggtcgac gacggcgtca agcggccagc
     1081 cggggccgga ctcgaggcgg ctgaggtaga agcgcacggc cgagtacggg caggtcggct
     1141 cgaccacgca gtcgaggcag cggtcggcgg cgccggcggg ccggtgctcg ggccggaagt
     1201 ggctgagccc gccgaagctg gccacccgcc ggatgcggcg gccgacgacg tactgcagcc
     1261 agtcgaggtc gtggcaggac ttggccatga gcatgaaggt ggccgcgtcc tggcggcgcc
     1321 aggggccgcg gacataggag tgggcctggt gcaggaaccc gaccggctcc aggtgctgga
     1381 cggagagcac ctgaccgagc cgtcccgcgt ccaccacctg cttcaccagg gccgtgtagg
     1441 gcgtgtagcg caggacgtgg cagacggcca ggatcacgcc ggcccgctcg gccgcctcga
     1501 cgatctgccg gcagccggcc tcggtggtgg ccatcggctt ctcgaggagc acgtggtagc
     1561 cgagcccggc gaaggcgatc gccggctcga cgtgcatggc gtcctgggtg cagacgagga
     1621 cggcgtcggc cgtccggcca cggccggcca gctcccgcca gtcggcgaag accgccccgg
     1681 cggggaggcc atggccggcg gcgaaccgga cccgacgggc ctggcggggc tcggcgaccg
     1741 cgaccacccg ggcccggtcc gggttggcca gcgcccagtc gccgtgggtg gtcccgcggt
     1801 tgccggcacc gacgacggcc agggtgacgg gcggcatcgc ggctcctagg cgccggcgcc
     1861 ctcgggcagg gcccgcaggt cggccggggt gggcagggac gcctgggcgc ccggacggag
     1921 gcaggccgcg gccgcgaccc gcgccgcccg ccgggccgcg tcctccaggg cggcgccggc
     1981 ggccagcgcg tcggccaggc cgccgcagaa ggcgtcgccg gcagcggtcg tatccaccgc
     2041 ccggacgggg acggcgggga cgtggccggc gtcgccgtcc tccacgacca gggcaccgtc
     2101 ggcccccagc gtgaccacca cggcccgggc cgggagccgg ccggccagct ccacggcctc
     2161 cgcgaccgtc gccgggaccg ccccgccggc cagctgggcc agctcgaccc ggttcggcac
     2221 cagcacgtcg
//
LOCUS       DASSWR010000451         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1372948, whole genome shotgun sequence.
ACCESSION   DASSWR010000451 DASSWR010000000
VERSION     DASSWR010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372948"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFO47_07545"
     CDS             complement(<1..486)
                     /locus_tag="VFO47_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET8788585.1"
                     /translation="MGSLPAREAYRMRWPRQLQGERRGCNADGGYGHRPGDNPGPWEA
                     ATDDSELIAASLVEPARFAELFDRHFPAIHRFLRRRVGAELADDLAAETFTQAFARRR
                     HSRAEHPDAGPWLYGIAGNLVRQHVRAEQRRLRACERVPPDLDTHDPGDGVAERVDAS
                     AA"
     gene            1052..2287
                     /locus_tag="VFO47_07550"
     CDS             1052..2287
                     /locus_tag="VFO47_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET8788586.1"
                     /translation="MERLIERCAGLDVHKASVTATVRVPGDQGGRQTETRTFRATTAG
                     LVLLGDWVASFGVTVVGMESTGVYWKPVYYLLEDDFEVWLLNAQHLKNVPGRKTDVAD
                     SVWICQLVEHGLVRPSFVPPKPIRELRDLTRYRKALLGERTREVQRLHKVLEDAGIKL
                     ASVASDPLGVSGRAMLEALVAGTHDPQVLAELARGRLRAKLPALREALEGRFGAHHGL
                     LVGEMLARIDQMDETIGRLSAEVARVAAPFSPLLALLMTIPGVSRRTAEVIVAEIGSD
                     MGRFPTAAHLASWAGVCPGNHESAGKHGSGRTRKGSKWLRVALVEAAHAAARTKNSYL
                     AAQYARLRGRRGPKKAALAVAHSVLVIAWHLLSRGEPYTDLGADYFVKRQAHQAYRDR
                     LVRQLERMGHKVTLEPTAA"
BASE COUNT          386 a          795 c          910 g          402 t
ORIGIN      
        1 cgcggcggac gcgtcgaccc gctcggccac accgtcgccg gggtcgtgtg tgtccaggtc
       61 gggtgggacg cgttcacagg ctcgcaggcg ccgctgctcg gcgcgtacgt gctggcgcac
      121 caggttccca gcgattccgt acagccatgg gcccgcgtcc ggatgctcag cccgggagtg
      181 ccggcgccgg gcgaacgcct gggtgaaggt ctcggcggcg aggtcgtcgg ccagctccgc
      241 accaaccctg cgtcgcagaa agcggtggat cgccgggaag tgccgatcga acagctcagc
      301 aaagcgtgcc ggctccacca gcgacgcggc gatcagctcg gaatcgtccg ttgctgcctc
      361 ccatggtccg gggttgtcac ccggtctatg accgtagccg ccgtcggcgt tgcacccacg
      421 ccgttccccc tgcagttggc gaggccaacg catccggtac gcttctctgg ccggcaggct
      481 gcccacatcc gacacgccat cgtgtcaacg gggcgacagc gcccggattc ctggcccgtg
      541 ccgacgaacc agacgaggcc cgagggcccg ggcggctcgg aacagcttcc cagaggccga
      601 gcggcttctg ctcgaaggtc tggacacggc gatggcagcc aacgaagaac ggaccgtgat
      661 tgatttcgat ccgtggaagg ctgagagcgc ttgaagaagt cgaataggtc tgggtgggat
      721 ccaggaggcc agcagcctat tgaacgacgg atatggagca cattactttg gcggtctacg
      781 gtcggccgcg acctgcagaa gcgccggtcc gcacagtggt gggtgcgaaa ttcatggtaa
      841 gtggctcatg gcctgcggcc acccctgacg gatgaaaata ggtcggtcgt ccgggtgacg
      901 tcggctcatc ctggtgcggt cgagcccgtt ggtaagtggg acgcgggagc ctggggcacc
      961 ccgaactgtt gctgcgagca cggacctgag actcttgatc atcaggctcc tccggacggc
     1021 accgttagtg tcgagcggga ggaggcaggc ggtggagcgg ctgattgagc gctgcgccgg
     1081 gctggacgtg cacaaggcat cggtcaccgc gacggtgcgg gtgccgggcg accaaggcgg
     1141 acgccagacc gagaccagga cgtttcgggc cacgaccgcg gggctggtgc tgctggggga
     1201 ctgggtggcc agcttcgggg tgaccgtggt cgggatggag tcgaccgggg tctattggaa
     1261 gccggtgtac tacctgctgg aggacgactt tgaggtgtgg ctgctgaacg ctcagcacct
     1321 caagaacgtc cccggccgca agacggatgt ggccgattcg gtgtggatct gccagctggt
     1381 cgagcacggg ctggtccgcc cgtcgttcgt gccgcccaag ccgatccggg agctgcggga
     1441 tctgacccgc taccgcaagg cgctgctggg cgagcgcacc cgggaggtcc agcggctgca
     1501 caaggtgctg gaggacgccg ggatcaagct ggccagcgtg gccagtgacc cgctgggcgt
     1561 ctctggccgg gcgatgctgg aggcgctggt ggccggcacc catgaccccc aggtgctggc
     1621 cgagctggct cgggggcggc tgcgggccaa gctgccggcg ttgcgggagg ccttggaggg
     1681 ccgctttggg gcccatcatg ggctgctggt cggggagatg ctggcccgca tcgaccagat
     1741 ggacgagacg atcgggcgcc tcagtgcgga ggtggcccgc gtggccgccc ctttctcgcc
     1801 gctgctggcg ttgctgatga ccatcccggg ggtgagccgg cgcaccgctg aggtgatcgt
     1861 ggccgagatc ggttcggaca tgggccggtt cccgaccgcc gcccacctgg cctcctgggc
     1921 cggggtgtgt ccaggcaacc acgagtcggc cggcaagcat ggctcgggtc ggacccgcaa
     1981 gggatccaag tggctgcggg tcgcgctggt cgaggcggcg catgcggccg cgcggaccaa
     2041 gaacagctac ctggccgccc agtacgcccg gctgcggggt cgccggggtc ccaagaaggc
     2101 ggcgctggct gttgcccact cggtcctggt gatcgcctgg catctgctca gccggggcga
     2161 gccctacacc gacctgggcg ccgactactt cgtcaagcgt caggcccatc aggcctaccg
     2221 ggaccggctg gtacggcaac tcgagcgcat gggccacaag gtcaccctgg agccaacagc
     2281 cgcctgaccg gatcccgggt gctggctggt cggctcctca gggcattttc acctgaggat
     2341 ggggtgaact ctcagcatag ccgccggaga gtttcactga atctgaagcg gccgccggga
     2401 cacacaagcg gcccactgct gcgaagccac gcagatcatt ccccgctttc tggccgctct
     2461 cgtctggtct cgccacaatc ctctgggtgc ttc
//
LOCUS       DASSWR010000452         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1006463, whole genome shotgun sequence.
ACCESSION   DASSWR010000452 DASSWR010000000
VERSION     DASSWR010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006463"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFO47_07555"
     CDS             complement(<1..356)
                     /locus_tag="VFO47_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8788587.1"
                     /translation="MAHRFADVDDVTQRLAKAHYLATPMIANVAFLADRLEKPVLAEG
                     PAGVGKTELAKALAAATGARLIRLQCYEGLDESKALYEWNYKKQLLRIQADRAHDASW
                     ADVEVDIFSEPFLLTRP"
     gene            complement(356..1369)
                     /locus_tag="VFO47_07560"
     CDS             complement(356..1369)
                     /locus_tag="VFO47_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8788588.1"
                     /translation="MSAPGAVAPAGLRVQRATRSSRIGLAVGAAAVVVLALGPLWALD
                     SVLRTLISFLTLLALAQMWNLLAGYAGLVSIGQQAYIGLGAYALLLFADTLGLPLLPS
                     VLAAGVAGTLIAVPTALLAFRLRGGYFAIGTWVIAEVYRLVITNVSQLGGGSGLTLRA
                     ASAFDRTTRLYLTYELALAVGVGAVAVTYLLLRRRLGLALTAIRDSEQSAQSLGVPVF
                     RAKLVAYLVAAFGCSLAGAVIYLNLLRVQPEAAFSVNWTAFMIFIVVIGGVGTIEGPI
                     IGTIVFFVLQETLSGYGAWYLVLLGAVAVLVTVAARRGLWGLVADRTGWSLFPVQRRL
                     EER"
     gene            complement(1366..2238)
                     /locus_tag="VFO47_07565"
     CDS             complement(1366..2238)
                     /locus_tag="VFO47_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8788589.1"
                     /translation="MTWVNVVTQGLLLGGLYALFATGLSLMFGVMRLVNLAHGDLSIV
                     AAFLALVIVDGTGINPLWTLLVVVPAMAGLGYLLQRGLLNFALRGDDPLPPVLVTFGL
                     SIILQNLLLEVFSADSQGLDAGRVENASVRLSDGLAVGWFPLLTLVTAVAVLVGLQLL
                     LSRTQLGRSFRATSDDPEAASLVGLDHRHVFAMATAIALATVAVAGLFLGVRTTFTPS
                     IGPTRLIFAFEAVIIGGLGSIWGTLAGGLVLGVAQALGAQFSPGWGVLAGHLVFLAVL
                     AFRPTGLLGRTVPA"
     gene            complement(2235..>2922)
                     /locus_tag="VFO47_07570"
     CDS             complement(2235..>2922)
                     /locus_tag="VFO47_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788590.1"
                     /translation="DVTLAVDEGETVAVIGANGAGKSTLLRTVAGLQDRVTGTVAFDG
                     APLDGVPTHRRLGLGIAMVPEGRRIFPSLTVEENLKVGVVRGRSGRWDVASVLELFGA
                     LADKRGRPGSLLSGGEQQMLAIGRALMANPRLLLLDEVSLGLAPLVVRRIYETLPEIT
                     AAGATVVLVEQDIGQALAGADRVYCLLEGRVALQGRPAELTREQITDAYFGLGGSGGS
                     SRAPRWDGDA"
BASE COUNT          474 a         1055 c         1077 g          316 t
ORIGIN      
        1 ggccgggtca gcaggaacgg ctcggagaag atgtcgacct cgacatcggc ccagctggcg
       61 tcatgggccc ggtcggcctg gatgcgcagc agctgcttct tgtagttcca ctcgtacagg
      121 gccttggact cgtccaggcc ctcatagcac tggagccgga tgagccgggc cccggtggcg
      181 gcggccaggg ccttggccag ctcggtcttg cccaccccgg ccggcccctc ggccagcacc
      241 ggcttctcca gccggtcggc caggaaggcg acgttggcga tcatcggggt ggccaggtag
      301 tgggccttgg ccaggcgctg ggtcacgtcg tcgacgtcgg cgaaccggtg ggccatcagc
      361 gctcctcgag tctccgctgg accgggaaca ggctccagcc ggtgcggtcg gcgaccagcc
      421 cccacagccc ccgccgggcc gccacggtga ccaggaccgc gaccgcgccg agcaggacca
      481 ggtaccaggc accgtacccg gagagggtct cctgcaacac gaagaacacg atcgtaccga
      541 tgatcggccc ctcgatcgtg cccacgcccc cgatgaccac gatgaagatc atgaaggcgg
      601 tccagttgac gctgaaggcc gcctccggct gcacccggag caggttcagg tagatgacgg
      661 ccccggccag cgagcagccg aacgccgcca ccaggtaggc gacgagcttg gcccggaaca
      721 cgggcacgcc aaggctctgg gcgctctgct cgctgtcgcg gatggcggtc agggccaggc
      781 cgaggcggcg ccgcaggagg aggtaggtga cggccaccgc cccgaccccg acggccaggg
      841 cgagctcgta ggtgaggtac aggcgggtgg tgcggtcgaa ggccgacgcc gcccgcaggg
      901 tcaggcccga gccgccgccg agctgggaga cgttggtgat caccagccgg tagacctcgg
      961 cgatgaccca ggtgccgatg gcgaagtagc cgccccgcag gcggaaggcg agcagcgccg
     1021 tcggcaccgc gatcagggtg ccggccaccc cggcggccag caccgagggc agcaggggca
     1081 ggccgagggt gtcagcgaac agcagcaggg cgtaggcgcc gaggccgatg taggcctgct
     1141 ggccgatgga gaccaggccg gcgtagccgg cgagcaggtt ccacatctgg gccagggcga
     1201 gcagggtcag gaagctgatc agggtgcgca gcacgctgtc cagggcccac agcggcccaa
     1261 gggcgagcac gaccaccgcg gccgccccca cggccaggcc gatccggctg gaccgggtgg
     1321 cccgctggac ccgcaggccc gccggcgcca ccgccccggg cgcgctcatg ccgggaccgt
     1381 cctgccgagc aggccggtgg gccggaaggc gagcacggcc aggaagacca ggtggccggc
     1441 caggaccccc cagccggggg agaactgcgc ccccagggcc tgggcgaccc cgaggacgag
     1501 cccgccggcg agcgtgcccc agatggaccc gagcccgccg atgatcaccg cctcgaaggc
     1561 gaagatcagc cgggtcgggc cgatgctggg ggtgaaggtg gtccgcaccc ccaggaacag
     1621 cccggccacg gccacggtgg ccagggcgat ggcggtggcc atggcgaaca cgtgccggtg
     1681 gtcgagcccg accaggctgg ccgcctcggg gtcgtcggag gtggcccgga acgagcggcc
     1741 gagctgggtc cggctgagca gcagctgcag gccgaccagg acggccacgg cggtgaccag
     1801 ggtcagcagc gggaaccagc ccacggccag gccgtcgctc agccgcacgc tggcgttctc
     1861 gacccggccg gcgtccagcc cctgggagtc ggccgagaac acctccagga gcaggttctg
     1921 gaggatgatc gagagcccga aggtgaccag caccggcggc agcgggtcgt cgccgcgcaa
     1981 ggcgaagttc agcagccccc gctggagcag gtagccgagc ccggccatgg ccggcaccac
     2041 caccagcagg gtccacagcg ggttgatccc ggtgccgtcg acgatcacca gggccaggaa
     2101 cgcggccacg atcgacaggt cgccgtgggc caggttgacc aggcgcatca ccccgaacat
     2161 gagcgacagc ccggtggcga acagggcgta cagccccccg aggagcagcc cctgggtgac
     2221 gacgttgacc caggtcatgc gtccccatcc catcgggggg ctcggcttga gccccccgaa
     2281 ccccccagcc cgaagtaggc gtcggtgatc tgctcgcggg tcagctcggc cggccggccc
     2341 tggagggcga cccggccctc gagcaggcag tagacgcggt cggccccggc cagggcctgg
     2401 ccgatgtcct gctcgaccag gaccacggtg gccccggcgg cggtgatctc gggcagggtc
     2461 tcgtagatcc gccgcaccac cagcggggcc agccccaggg agacctcgtc cagcagcagc
     2521 agccgcgggt tagccatcag ggcccgcccg atggccagca tctgctgctc gcccccggac
     2581 aggaggctgc ccggccggcc acgcttgtcg gccagggcgc cgaagagctc cagcaccgag
     2641 gccacgtccc agcggccgga ccggccccgg accaccccaa ctttcaagtt ctcctcgacg
     2701 gtgagactcg ggaagatgcg ccggccctcg gggaccatgg cgatgccgag gcccaggcgc
     2761 cggtgggtcg ggaccccgtc cagcggcgcc ccgtcgaagg ccacggtgcc ggtgacccgg
     2821 tcctggaggc cggccacggt ccgcagcagg gtcgacttgc cggccccgtt ggccccgatc
     2881 acggccaccg tctcgccctc gtcgaccgcc agcgtcacgt cg
//
LOCUS       DASSWR010000453         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_243581,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000453 DASSWR010000000
VERSION     DASSWR010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243581"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFO47_07575"
     CDS             <1..273
                     /locus_tag="VFO47_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788591.1"
                     /translation="LAGGLAADLLLARLRPGPDRPGPFRAAGALVPVLLWGAWLAVYA
                     LAYGIAWPPELWAGVLGMASLSGLGLSILVLPPAVPEAAWKPISKA"
     gene            359..1798
                     /locus_tag="VFO47_07580"
     CDS             359..1798
                     /locus_tag="VFO47_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8788592.1"
                     /translation="MADSGSRSGRLWTPDRRQFLRAVGVAGMGAAIAACSSEPGGGGP
                     GGGQEAAGQAPSVSFTEPRTKLSGDLKILLWSHFVPSHDQWFRPFAQDWGKKVGVNVT
                     VDHIDNAQIPTRIAAEIQAGQGHDVIQYIAPLSQFEPSVLDLKDITDEATKRWGQQLE
                     LSKQSSFNPATGRYYAYSPGWVPDPGDYRTSLWTAVGMPKGPTTWDELLEGGAEIKKT
                     KGTQLGIGMSQEIDSNMAGRALMWSYGASVQDENERVVINSEETVAAVEFMTKLFKGA
                     MTDEVFSWNAASNNQGLVAGKLSYILNSISAWRTAQGANPDVANDIGFVPALRGPADA
                     RAAQHVLYNWIIPKHAANPDAAKEFLLYYTENFASATWHSKLYDFPAWAERVPQLDGW
                     LAKDPYGAQPADKLAFLKDATDWSTNIGYPGPSNTAIGEVFGTFVIPNMFASAARGEA
                     SPKEAVAQAEAQIKPIFEKWRKRGLVGGT"
     gene            1812..2855
                     /locus_tag="VFO47_07585"
     CDS             1812..2855
                     /locus_tag="VFO47_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788593.1"
                     /translation="MTKVVEVRGLVKQFEGGRVHAVDGIDLATEEGEYLVLLGPSGCG
                     KTTLLRTIAGLEEPSEGEVFIDGQVVNDLPPRARKIAMVFQSYALYPHKNVLQNIVFP
                     LKAERMDKDEQVRKARWAAELLGIDQLLDRRPRQLSGGERQRVALARAMVRDPHVFLL
                     DEPLSNLDAKLRTSARDELKRFQQRVGTTTIYVTHDQAEAMGLGDRIAVMDHGRVRQL
                     GPPVEVYDDPADTFVATFIGSPPMNLVPRDGRFVGFRPEHLLPAEHVGGPERTLVPFK
                     VDRIEYLSGDRHVYGTATRIGEETRVIARLPATVPTPIAVGETHEFAVHPDRLRFFDA
                     ESGHRADPVPVGG"
     gene            2860..3795
                     /locus_tag="VFO47_07590"
     CDS             2860..3795
                     /locus_tag="VFO47_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8788594.1"
                     /translation="MDAATTERPGVRAGRRPARHFADNDRQMAIWFLVPSIVYVVALV
                     AVPFFLAIGFAFSDVTAGDPSFHWAGLRTFQRALDDPVFWRSLRNTFVFTIVSMALIV
                     VLGKVLANILVADFRGKWLVRFLVLLPWTTPVALSAVAWLWMLDSVFSPIDWVLRNLG
                     LIDANIYWLGRPHLAMASVIAVHVWRLVPLSAVIILAGLVAIPKDIEEAARVDGAGFW
                     RRMFEVTIPLTLPVVAVAALFGAILTFTDMTVVYVLTRGGPNHATQVLASWAFIRGIE
                     GGDVAQGAAVALFLFPLLLAAAIAILRAVRRMEVL"
     gene            3796..>4626
                     /locus_tag="VFO47_07595"
     CDS             3796..>4626
                     /locus_tag="VFO47_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8788595.1"
                     /translation="MVATAEVPKGVAAERLRRHYRRRHVMARVGLYVAAVLAAFICAA
                     PFLWSLVTAFKQNRDLYNPENSPFFFNLAATPDHLLYLLRDTAFLTFIWNTLWVGLLV
                     VAITLLLGLPAAYSLARLDRPWSGPMAIAIFFVYLVPPSMLFLSLSRLVVTTGLQDST
                     WSLVLVYPTITIPVSVWLLIGFLKAIPRDVEEQAMVDGYSRLAAFVRVVLPLAFPGIV
                     AVVVFAVTLTASEVIYALAFVSPTTEKVVSTGVPTELIRGDVFFWQSLQAAAILVAVP
                     I"
BASE COUNT          649 a         1719 c         1550 g          708 t
ORIGIN      
        1 ctggccggcg gcctggccgc cgacctgctg ctggcccggc tgcgccccgg gcccgaccgg
       61 cctggcccct tccgggccgc cggcgccctg gtcccggtgc tgctctgggg cgcctggctg
      121 gccgtgtacg ccctcgccta cgggatcgcc tggccgcccg agctgtgggc cggcgtgctc
      181 ggcatggcct cgctcagcgg cctcggcctg agcatcctgg tcctgccgcc ggccgttccc
      241 gaagcggcct ggaaaccgat ttcaaaggca taagcacgcc gaacttgggt aaagcgcttc
      301 tttggacggc ggggtgggaa acgccgtccc aagctctgga ccaagggagg ctgcgcacat
      361 ggccgacagc ggatcccgat caggtcgcct gtggacaccc gaccggcgac agttcctgcg
      421 ggcggtgggg gtcgccggca tgggggcggc gatcgccgcc tgctccagcg agccgggcgg
      481 gggaggcccg ggcggcggac aggaggcggc cggccaggcg ccgtcggtca gcttcaccga
      541 gccgaggacc aagctctcgg gtgacctcaa gatcctgctc tggagccact tcgtgcccag
      601 ccacgaccag tggttccgcc ccttcgccca ggactggggc aagaaggtcg gggtcaacgt
      661 gaccgtcgac cacatcgaca acgcccagat cccgacccgg atcgcggccg agatccaggc
      721 cggccagggt catgacgtga tccagtacat cgcgccgctg tcgcagttcg agcccagcgt
      781 cctcgacctc aaggacatca ccgacgaggc caccaagcgc tggggccagc agctggagct
      841 gagcaagcag tccagcttca acccggccac cggccgctac tacgcctact cgcccggctg
      901 ggtgcccgac cccggcgact accggacctc gctgtggacg gctgtgggca tgcccaaggg
      961 ccccaccacc tgggacgagc tcctggaggg cggcgccgag atcaagaaga ccaaggggac
     1021 ccagctcggc atcggcatgt cccaggagat cgactccaac atggccggcc gggccctgat
     1081 gtggtcctac ggcgcctcgg tccaggacga gaacgagcgg gtggtcatca actccgagga
     1141 gacggtggcc gccgtcgagt tcatgaccaa gctgttcaag ggggccatga ccgacgaggt
     1201 cttctcctgg aacgccgcct ccaacaacca gggcctggtc gccggcaagc tctcctacat
     1261 cctcaactcg atctcggcct ggcggaccgc ccagggggcc aaccccgacg tggccaacga
     1321 catcggcttc gtcccggcgc tgcgcgggcc cgccgacgcc cgcgccgccc agcacgtgct
     1381 ctacaactgg atcatcccca agcacgcggc caaccccgac gcggccaagg agttcctgct
     1441 ctactacacc gagaacttcg cctcggccac ctggcactcc aagctgtacg acttcccggc
     1501 ctgggccgag cgggtgcccc agctcgacgg ctggctggcc aaggacccct acggggccca
     1561 gccggccgac aagctggcct tcctcaagga cgcgaccgac tggagcacca acatcggcta
     1621 cccggggccg tccaacaccg ccatcggcga ggtgttcggg accttcgtga tcccgaacat
     1681 gttcgccagc gcggcccgcg gcgaggcctc gcccaaggag gccgtggccc aggccgaggc
     1741 ccagatcaag ccgatcttcg agaagtggcg gaagcgcggc ctggtcggcg gcacgtgatc
     1801 ggcggccggc cgtgaccaag gtcgtggagg tccgcgggct cgtcaagcag ttcgagggcg
     1861 ggcgggtcca cgccgtggac ggcatcgacc tggccaccga ggagggcgaa tacctcgtcc
     1921 tcctcggccc ctcggggtgc ggcaagacca ccctgctgcg caccatcgcc gggctggagg
     1981 agccgagcga gggggaggtc ttcatcgacg ggcaggtcgt caacgacctg ccgccgcggg
     2041 cccgcaagat cgccatggtc ttccagagct acgccctgta cccgcacaag aacgtcctcc
     2101 agaacatcgt cttcccgctc aaggccgagc ggatggacaa ggacgagcag gtccgcaagg
     2161 cccgctgggc ggccgagctg ctcggcatcg accagctgct ggaccgccgg ccccgccagc
     2221 tctcgggcgg cgagcgccag cgggtcgccc tggcccgggc gatggtccgc gacccccacg
     2281 tgttcctgct cgacgagccg ctctccaacc tcgacgccaa gctgcgcacg agcgcccgcg
     2341 acgagctcaa gcgcttccag cagcgggtcg ggacgaccac catctacgtg acccacgacc
     2401 aggccgaggc catgggcctg ggcgaccgga tcgcggtcat ggaccacggc cgggtgcgcc
     2461 agctcggccc gccggtcgag gtctacgacg acccggccga caccttcgtg gccaccttca
     2521 tcggctcccc gccgatgaac ctggtccccc gcgacggccg cttcgtcggg ttccgccccg
     2581 agcacctgct gccggccgag cacgtcggag ggcccgagcg aacgctggtg ccgttcaagg
     2641 tggaccggat cgagtacctc tccggcgacc ggcacgtgta cggcacggcc acgcgcatcg
     2701 gcgaggagac ccgggtcatc gcccggctgc cggccacggt cccgaccccg atcgccgtcg
     2761 gggagaccca cgagttcgcc gtgcaccccg accggctgcg gttcttcgac gccgaaagcg
     2821 ggcaccgggc cgaccccgtc ccggtcggag gctgaggcca tggacgcggc caccaccgag
     2881 cgccccggcg tccgcgccgg gaggaggccg gcccggcact tcgccgacaa cgaccgccag
     2941 atggcgatct ggttcctggt gccgtcgatc gtgtacgtgg tcgcgctcgt ggcggtgccg
     3001 ttcttcctgg ccatcgggtt cgccttcagc gacgtgaccg ccggcgaccc cagcttccac
     3061 tgggccggcc tgcgcacctt ccagcgcgcc ctcgacgacc cggtgttctg gcgctcgctg
     3121 cgcaacacct tcgtcttcac catcgtctcg atggccctga tcgtggtcct gggcaaggtc
     3181 ctggccaaca tcctggtggc cgacttccgc ggcaagtggc tggtccggtt cctggtcctg
     3241 ctgccctgga cgaccccggt ggccctgtcg gcggtggcct ggctgtggat gctggactcg
     3301 gtcttcagcc cgatcgactg ggtgctgcgc aacctgggcc tgatcgacgc caacatctac
     3361 tggctgggcc ggccgcacct ggccatggcg tcggtgatcg ccgtccacgt gtggcggctg
     3421 gtcccgctgt cggcggtcat catcctggcc gggctggtcg ccatccccaa ggacatcgag
     3481 gaggcggccc gggtcgacgg ggccggcttc tggcggcgca tgttcgaggt gaccatcccc
     3541 ctgaccctgc ccgtggtcgc ggtcgcggcc ctgttcgggg ccatcctgac cttcaccgac
     3601 atgacggtgg tgtacgtcct cacccgcggc ggccccaacc acgccaccca ggtgctggcc
     3661 agctgggcgt tcatccgcgg catcgagggc ggcgacgtcg cccagggggc ggcggtggcg
     3721 ctgttcctgt tcccgctgct gctggccgcg gccatcgcca tcctgcgggc ggtccggcgc
     3781 atggaggtgc tgtaggtggt cgccaccgcc gaggtgccca agggcgtcgc ggccgagcgg
     3841 ctccggcggc actaccggcg ccgccatgtc atggcccggg tcggcctcta cgtggcggcg
     3901 gtgctggccg ccttcatctg cgccgccccg ttcctgtgga gcctggtcac cgcgttcaag
     3961 cagaaccgcg acctctacaa ccccgagaac agcccgttct tcttcaacct ggccgccacc
     4021 cccgaccacc tgctgtacct gctgcgcgac accgccttcc tgaccttcat ctggaacacc
     4081 ctgtgggtcg ggctgctggt cgtggccatc acgctgctgc tggggctgcc ggccgcctac
     4141 agcctggccc ggctcgaccg gccctggtcg gggccgatgg ccatcgccat cttcttcgtc
     4201 tacctggtcc cgccgagcat gctgttcctg tccctgtcgc ggctggtggt gaccaccggc
     4261 ctgcaggact cgacctggtc gctggtgctg gtctacccga ccatcaccat cccggtgtcg
     4321 gtgtggctgc tgatcgggtt cctcaaggcc atccccaggg acgtcgagga gcaggccatg
     4381 gtcgacggct acagtcgcct ggccgcgttc gtgcgggtcg tcctcccgct ggccttcccc
     4441 ggcatcgtgg ccgtggtggt gttcgccgtc accctcacgg ccagcgaggt catctacgcc
     4501 ctggccttcg tctcgcccac caccgagaag gtcgtctcga ccggcgtccc caccgagctg
     4561 atccgcggcg acgtgttctt ctggcagtcg ctgcaggcgg cggcgatcct ggtcgcggtc
     4621 ccgatc
//
LOCUS       DASSWR010000454         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_722611,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000454 DASSWR010000000
VERSION     DASSWR010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722611"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFO47_07600"
     CDS             complement(<1..473)
                     /locus_tag="VFO47_07600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8788596.1"
                     /translation="MTESMPDPVDCADRLAELAGPGPALELGIGTGRVALPLAARGVE
                     VHGIDASAAMVERLRAKPGGAAIPVTMGDFADLAVEGSYRLVYAVFNTFFSLLTQDDQ
                     VRCFEAVAGHLAPEGAFVLELFVPDPTMHPGGQSVRTRHLGLDRARLDLALHDPVA"
     gene            complement(661..1320)
                     /locus_tag="VFO47_07605"
     CDS             complement(661..1320)
                     /locus_tag="VFO47_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788597.1"
                     /translation="MVHDRLLRLLQDAATGSPPPADGLVEVWPPPPGAVDAVLAFTAH
                     HVVAARVDPGLVAARLPPGDLEAPMSAAFLGWLGERLGSQPGSLDVVLAAEGLGGTPP
                     LALRAGVDPERHPRVARALRYRDEPAAWTDADGLGVLVLGRGLAGRREVAFEVDPAAR
                     NRGLGRLLVTAARHLTPPGEPLFAQVAPGNVASLRVVGAAGFRPVGAEVLFHRAGASP
                     G"
     gene            1430..>2005
                     /locus_tag="VFO47_07610"
     CDS             1430..>2005
                     /locus_tag="VFO47_07610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8788598.1"
                     /translation="MQQPSRPTEPRQEIVDLAAGLVPALAGRAAGYDEADAFCHEDFD
                     DLVAAGYTAITVPAELGGMGASALDLVAAQSRLAEGNPATALAVNMHLHGVGLLAEGF
                     RERVEPFLKLVATDGAILAGGFSEPQSGGNWWYQATTATPLPGGGYRLSGTKTFFTGW
                     PRATHLFLTAAVEVDGDREPIGFLVPRPERGV"
BASE COUNT          254 a          760 c          759 g          232 t
ORIGIN      
        1 gccaccgggt cgtgcagggc caggtcgagc cgggcccggt ccaggcccag atggcgggtc
       61 cggacgctct ggccgccggg gtgcatggtc gggtcgggca cgaacagctc gaggacgaag
      121 gcgccctccg gggccaggtg accggccacc gcctcgaagc agcggacctg gtcgtcctgg
      181 gtgagcagcg agaagaaggt gttgaagacc gcgtagacca gccggtagct gccctccacg
      241 gccaggtcgg cgaagtcgcc catggtgacg gggatggccg cgccgcccgg cttggcccgc
      301 aaccgctcca ccatggccgc cgaggcgtcg atgccgtgga cctcgacccc cctggccgcc
      361 aggggcaggg ccacccggcc ggtgccgatg cccagctcca gggccggccc gggaccggcc
      421 agctcggcca gccggtccgc acagtccacc gggtccggca tgctctcggt catggtgtcg
      481 tagacctcgg cgatccggtc accgtaggtc tcggctcggt agccctccat gggcccattc
      541 cagcagcccc ggcgcccatg gcacgcgaaa ttgcccggag cggcggtatc gttggcccat
      601 ggcgatgatg accgggctgg gcccgttcgg gcacgccccc gcgggccgcg gatctgacgg
      661 ctagcccggg ctggcgccgg cgcggtggaa caggacctcg gcgccgacgg ggcggaagcc
      721 ggccgccccg accacccgca gggaggcgac gttgccgggg gcgacctgcg cgaacagcgg
      781 ctcgccgggt ggggtgaggt ggcgggcggc cgtgaccagg aggcggccga ggccgcggtt
      841 gcgggcggcc gggtcgacct cgaaggcgac ctcgcggcgg ccggccaggc cccgccccag
      901 caccagcacc ccgagcccgt ccgcgtcggt ccaggccgcc ggctcgtccc ggtagcgcaa
      961 cgccctggcc acccgggggt gccgttccgg gtcgaccccg gcccgcagcg ccagtggcgg
     1021 ggtcccgcca aggccctcgg cggccaggac cacgtcgagg ctgcccggct ggctgcccag
     1081 ccgctccccg agccagccca ggaaggccgc gctcatcggc gcctccaggt ccccgggcgg
     1141 cagccgcgcc gccaccaggc cggggtcgac ccgggccgcg accacgtggt gggcggtgaa
     1201 cgccagcacc gcgtccaccg ccccgggcgg cgggggccac acctcgacca gcccgtccgc
     1261 cggcggcggc gaccctgtcg ccgcgtcctg gagcagccgc aacagccgat cgtgcaccat
     1321 acgagcctac ccacttccgg cggtcgggcc ggcatcgttc gtgtttgcgg cgggttcgtg
     1381 gaaggctgtt ggggtaggca cggccgagcg tcctcgtcca ggagcagtca tgcagcagcc
     1441 atcgcgaccc accgagcccc ggcaggagat cgtcgacctg gccgcgggtc tggtccccgc
     1501 cctggccggc cgcgccgccg gctacgacga ggccgacgcg ttctgccacg aggacttcga
     1561 cgacctggtc gcggccggct acactgccat caccgtcccg gccgaactcg gcggcatggg
     1621 ggcgagcgcg ctcgacctgg tcgccgccca gagccggctg gccgagggca acccggccac
     1681 cgccctggcc gtcaacatgc acctgcacgg ggtcggcctg ctggccgagg ggttccggga
     1741 gcgggtggag ccgttcctca agctggtcgc gaccgacggg gccatcctgg ccggcggctt
     1801 ctccgagccc cagtcgggcg ggaactggtg gtaccaggcc accaccgcga ccccgctgcc
     1861 ggggggcggc taccggctgt ccgggaccaa gacgttcttc accggctggc cgcgggccac
     1921 ccacctgttc ctgacggcgg cggtcgaggt cgacggcgac cgggagccga tcgggttcct
     1981 ggtgccgcgg ccggagcggg gcgtg
//
LOCUS       DASSWR010000455         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_751337,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000455 DASSWR010000000
VERSION     DASSWR010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751337"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFO47_07615"
     CDS             <1..854
                     /locus_tag="VFO47_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8788599.1"
                     /translation="RRHYRRRHVMARVGLYVAAVLAAFICAGPFLWSLVTAFKQNRDL
                     YNPENSPFFFNLAATPDHVLYLLRDTAFLTFVWNTLWVGLLVVAITLVLGLPAAYSLA
                     RLDRPWSGPMAIAIFFVYLIPPSLLFLSLSRLVVTVGLQDSTWSLVLVYPTITIPVSV
                     WLLIGFLKAIPRDVEEQAMVDGYSRLAAFVRVVLPLAFPGIVAVVVFAFTLTASEFIY
                     ALAFVSPTTEKVVSTGVPTELIRGDVFFWQSLQAAAILVAVPIAFVFNLFLDRFISGF
                     TMGAVKG"
     gene            940..2574
                     /locus_tag="VFO47_07620"
     CDS             940..2574
                     /locus_tag="VFO47_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8788600.1"
                     /translation="MDGPYGPRRIVYADYTASGRALAFIEDFIRHEVLPRYANTHTES
                     SGTGLQTTRLREDARRIIRDAVGGDDEVAVLFCGSGSTGAVDKLIGVLNLRIPADLDA
                     RYRLSERIPAGERPVVFTGPFEHHSNELPWRESIAEVVVIREDADGHIDLGQLEEELV
                     GHAGRPLRIGSFSAASNVTGIVSDTRAIASLLHRHGALSFWDFAAAGPYVQIDMGAPD
                     GDPLAYKDAIFLSPHQFIGGPGTPGVLVVRRELLRNRVPTVPGGGTVAYVNPTEHRYL
                     DDPVEREEGGTPAIVESIRAGLVFQLKEAVGAGTIQALERSFIRRAMASWGANPNIEL
                     LGNLQAERLSIVSFVVRHGGRYLHHNFVVALLNDLFGIQSRGGCSCAGPYGHRLLGID
                     IERSHSFEREIARGCEGIKPGWVRVNFNYFISEVVFDYLLEAVHLVADQGGKLLGDYR
                     FDPATGLWRHRRGPLEPPLRLHALHYDDQGRLNAAAAHATASDAALPGYLDQARRLLA
                     ARTPSGDGDGRGDGALPGLSADFEELRWFVLPRECL"
     gene            complement(2571..3209)
                     /locus_tag="VFO47_07625"
     CDS             complement(2571..3209)
                     /locus_tag="VFO47_07625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET8788601.1"
                     /translation="MQRAYGLEIPQTLEEVCDPRRMALLVYDMQDGIVRHLPDAAAFT
                     ARVVRVVEAARRARMRVIFARHLSLPNELAGVFQLRTAMAWQRVERVEDVRPLFLRDA
                     PDTRLVPELAPLPSEAVFDKLAMSMFVGTPLEMVLRDCGISAFAIVGAVTEIGIEPTV
                     RHGADLGFIPVVVADACGSVDPAAGERSLASLDYAKMSLQTDVDTFTRLLAG"
     gene            3363..4238
                     /locus_tag="VFO47_07630"
     CDS             3363..4238
                     /locus_tag="VFO47_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8788602.1"
                     /translation="MAEIDYSIRPLCYSHVDLPREFFGGVPIHSGEGVSTSPMVYVLV
                     SGKEPDGTVHHYLVDAGFAADKWIHRFGFYHWEAPEAVLAKVGVAPEDIEKVFLTHMH
                     FDHGNNLPAFPNAAVYVQWDEFQGWCEALALPQLYTPLGEESWVTSSFDRDDLQMYGR
                     LAGEQRLHYVRDGEEIVPGIVGHLSRDGHTFGSQWISIQTSGGPVVVAGDTVMWYSNV
                     EEMWPSGYTNGNTYKMLLTYGEIHEYVGGDLDRIVPGHDIELFKRHSAWKAGDNEVAE
                     VHVAGWDTSLRAGDA"
     gene            4242..>4489
                     /locus_tag="VFO47_07635"
     CDS             4242..>4489
                     /locus_tag="VFO47_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET8788603.1"
                     /translation="MTDRRVLWLEECDAGSGPLVGGKAVGLGHLVRLGLRVPPGFAVT
                     TAAYREALAGGGRPVTVGDRLAAEIAAAYRRLGGDGEVP"
BASE COUNT          600 a         1654 c         1541 g          694 t
ORIGIN      
        1 tgcggcggca ctaccggcgc cgccatgtca tggcccgggt cggcctctac gtggcggcgg
       61 tgctggccgc cttcatctgc gccggcccgt tcctgtggag cctggtcacc gcgttcaagc
      121 agaaccgcga cctctacaac cccgagaaca gcccgttctt cttcaacctg gccgccaccc
      181 ccgaccacgt cctgtacctg ctgcgcgaca ccgccttcct gaccttcgtc tggaacaccc
      241 tgtgggtggg gctgctggtg gtggccatca ccctggtgct gggactgccc gccgcctact
      301 ccctggcccg gctcgaccgg ccctggtcgg ggccgatggc catcgccatc ttcttcgtct
      361 acctgatccc gccgagcctg ttgttcctgt cgctgtcgcg gctggtcgtg accgtcggcc
      421 tgcaggactc gacctggtcg ctggtgctgg tctacccgac catcaccatc ccggtgtcgg
      481 tctggctgct gatcgggttc ctcaaggcca tccccaggga cgtcgaggag caggccatgg
      541 tcgacggcta cagccgcctg gccgccttcg tccgggtcgt gctgcccctg gccttccccg
      601 gcatcgtggc cgtggtggtg ttcgccttca ccctcacggc cagcgagttc atctacgccc
      661 tggccttcgt ctcgcccacc accgagaagg tcgtctccac cggcgtcccc accgagctga
      721 tccgcggcga cgtgttcttc tggcagtcgc tgcaggcggc ggccatcctg gtcgcggtcc
      781 cgatcgcatt cgtgttcaat ctgttcctcg accgcttcat cagcggcttc accatggggg
      841 cggtcaaggg ctgagggggt cgggttggac gcgggcgagg cggcagcgtc gccgctggtc
      901 gagcgggtcc gcgcgtcggt catcggcgac cgcgaggtga tggacgggcc gtacggcccg
      961 cgccggatcg tctacgccga ctacaccgcg tcgggccggg cgctcgcctt catcgaggac
     1021 ttcatccgcc acgaggtgct gccccgctac gccaacaccc acaccgagag ctcgggcacg
     1081 ggcctgcaga cgacccggct gcgggaggac gcccggcgca tcatccgcga cgccgtgggc
     1141 ggcgacgacg aggtggccgt gctgttctgc gggtcgggca gcaccggcgc cgtcgacaag
     1201 ctcatcggcg tcctcaacct gcgcatcccg gccgacctcg acgcccgcta ccggctctcg
     1261 gagcggatcc cggcggggga gcggccggtg gtgttcaccg ggccgttcga gcaccactcc
     1321 aacgagctgc cctggcggga gtcgatcgcc gaggtggtgg tgatccgcga ggacgccgac
     1381 ggccacatcg acctcggcca gctggaggag gagctggtcg gccacgccgg ccggccgctg
     1441 cggatcggga gcttctcggc cgcctcgaac gtcaccggca tcgtctccga cacccgggcc
     1501 atcgcgtcgc tgctgcaccg ccacggcgcc ctgtcgttct gggacttcgc cgccgccggc
     1561 ccctatgtcc agatcgacat gggggcgccc gacggcgacc cgctggccta caaggacgcc
     1621 atcttcctgt cgccccacca gttcatcggc ggccccggca cccccggggt cctggtggtc
     1681 cggcgcgagc tgctccgcaa ccgggtgccg acggtcccgg gcggcggcac cgtcgcctac
     1741 gtcaacccga ccgagcaccg ctacctcgac gacccggtcg agcgggagga gggcggcacc
     1801 ccggcgatcg tcgagtcgat ccgggccggc ctggtcttcc agctcaagga ggcggtcggg
     1861 gccggcacca tccaggccct ggagcggtcg ttcatccgcc gggccatggc gtcctggggg
     1921 gccaacccca acatcgagct gctcggcaac ctccaggccg agcggctgtc gatcgtgtcc
     1981 ttcgtggtcc gccacggcgg ccgctacctg caccacaact tcgtcgtggc cctgctcaac
     2041 gacctgttcg gcatccagtc gcggggcggc tgctcctgcg ccggccccta cggccaccgg
     2101 ctgctcggca tcgacatcga acggtcccat tccttcgagc gggagatcgc ccgcggctgc
     2161 gagggcatca agccgggctg ggtccgggtc aacttcaact acttcatctc cgaggtggtg
     2221 ttcgactacc tcctggaggc cgtccacctg gtcgccgacc agggcgggaa gctgctcggg
     2281 gactaccgct tcgacccggc caccggcctg tggcgccacc gccggggccc gctcgagccg
     2341 cccctgcgcc tgcacgccct ccactacgac gaccagggcc gcctgaacgc cgccgccgcc
     2401 cacgccaccg cctccgacgc cgcccttccc ggctacctcg accaggcccg ccggctgctg
     2461 gccgcccgca ccccctccgg cgacggcgac ggccgcggcg acggcgcgtt gcccggcctg
     2521 tcggccgact tcgaggagct gcgctggttt gtgctcccgc gggagtgcct ctagccggcc
     2581 agcagccggg tgaaggtgtc gacgtcggtc tgcagcgaca tcttggcgta gtccaggctg
     2641 gccagggagc gctcgccggc tgccgggtcc accgacccgc aggcgtcggc gaccacgacc
     2701 gggatgaagc ccaggtcggc cccatggcgg accgtcggct cgatgccgat ctcggtcacc
     2761 gccccgacga tcgcgaaggc gctgatcccg cagtcgcgca gcaccatctc caggggcgtg
     2821 ccgacgaaca tcgacatggc cagcttgtcg aacacggcct cgctgggcag cggcgcgagc
     2881 tcgggcacca gccgggtgtc gggagcgtcg cggaggaaca gcggccggac gtcctcgacc
     2941 cgctcgaccc gctgccaggc catggccgtc cggagctgga acaccccggc cagctcgttg
     3001 gggagcgaca ggtgcctggc gaagatcacc cgcatccggg cccgcctggc cgcctccacc
     3061 acccggacga ccctggccgt gaacgccgcc gcgtccggca ggtgccggac gatgccgtcc
     3121 tgcatgtcgt acaccagcag cgccatccgc cgcgggtcgc agacctcctc gagggtctgg
     3181 gggatctcca gtccgtaggc acgctgcatc gttcctcgcc ttcgtgagct gaccagccgt
     3241 ggcagtctag aggctctctc caagggcgaa gcttctgtcc ggtggcgccg gacacctggc
     3301 ttgctcgggc ggggtgcgcc ggaaagtctg gagcgtaccg gcgccgtctc gggaggttgg
     3361 ccatggcgga gatcgactac tccatccgtc cgctctgcta ctcgcacgtc gacctccccc
     3421 gggagttctt cggtggagtg ccgatccaca gcggggaggg cgtctcgacc tcgccgatgg
     3481 tctacgtcct ggtcagcggg aaggagccgg acgggacggt gcaccactac ctggtggacg
     3541 cgggcttcgc ggccgacaag tggatccacc ggttcggctt ctaccactgg gaggcgcccg
     3601 aggcggtgct ggccaaggtc ggggtcgccc ccgaggacat cgagaaggtg ttcctcaccc
     3661 acatgcactt cgaccacggc aacaacctgc ccgcgttccc gaacgcggcc gtgtacgtgc
     3721 agtgggacga gttccagggc tggtgcgagg ccctggccct gccccagctg tacaccccgc
     3781 tcggcgagga gagctgggtg acctcgtcgt tcgaccgtga cgacctgcag atgtacgggc
     3841 ggctggccgg tgagcagcgg ctgcactatg tccgcgacgg cgaggagatc gtccccggga
     3901 tcgtcgggca cctgagcagg gacgggcaca ccttcggcag ccagtggatc tcgatccaga
     3961 cctcgggcgg cccggtcgtc gtggcgggcg acaccgtcat gtggtactcg aacgtcgagg
     4021 agatgtggcc cagcggctac accaacggca acacctacaa gatgctgctg acctatggcg
     4081 agatccacga gtacgtgggc ggcgacctgg accgcatcgt ccccggccac gacatcgagc
     4141 tgttcaagcg tcacagcgcc tggaaggcgg gcgacaacga ggtcgccgag gtccacgtgg
     4201 ccggctggga cacctcgctg cgcgccggcg acgcctgacc gatgaccgat cgccgggtcc
     4261 tctggctgga ggagtgcgac gccggcagcg ggccgctggt gggcgggaag gcggtcgggc
     4321 tcgggcatct ggtccgcctg gggctgcggg tgccgcccgg gttcgccgtc accaccgccg
     4381 cctaccggga ggcgctggcc ggcggcggcc ggcccgtcac ggtcggcgac cggctggccg
     4441 ccgagatcgc cgccgcctac cggcgcctgg gcggggacgg ggaggtgcc
//
LOCUS       DASSWR010000456         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_159161,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000456 DASSWR010000000
VERSION     DASSWR010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159161"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..195)
                     /locus_tag="VFO47_07640"
     CDS             complement(100..195)
                     /locus_tag="VFO47_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8788604.1"
                     /translation="MVLTRLARFQRLDPGDLVVTGTPGGTARDPG"
     gene            complement(186..>2221)
                     /locus_tag="VFO47_07645"
     CDS             complement(186..>2221)
                     /locus_tag="VFO47_07645"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HET8788605.1"
                     /translation="QLQELARWLNPVTASPAAPRQTVAALDAFGPSLMPRTARLHGVA
                     MGLGMLGARATTGVVEKLTRLAVPADAPLPHQLAARAVIGGAGAALAAVPERHCQRLW
                     VASLRSTGQLLRDGAAGAAVHDLGRWVQRRYPSQRAVQPIAVAAIHTAGLLYRAGRRL
                     PAREAGVDRWPRPETATLPGALATSYAVTTVGTGLARGFVWSRGALESYFGPGPSKRV
                     LARLVNAGLWAGATMAVYNAGVAYVGRANQKVEPGYATAPASPLVSGCPESLLPFADL
                     GQQGRRFVTDVVTPELIEEVMGEPAIAQPIRTYVGFNSEPLYQTGRAELALAELDRAG
                     AFDRSYLLLISPTGTGWVDQTLIEAAEFLTRGDIATCCIQYARYPSFLSVQKVALGRR
                     QFRLLLWGVKQRLAGLPPEGRPRVLVFGESMGAWTSSDVLMYQGIDGFDHYGIDRALW
                     VGLPWLAKWSRSGMTRGSSTLVPHGTVEVFDRQEQLAELADEERARLRAVVLSHDNDP
                     IAVFGPDLLVQRPWWLADGQRGRGVPEQMRWLPLVTFLQTGMDAMNAMLKVPGEFTSF
                     GHDYRADMAGFVGDAYRLPQATAPQLAGIERTLRTLELERAERIKAEHSHAAPPAPAQ
                     RSGGDGIRGGVPLHSPRTRGARWIGRRGPSGRTGAAAAGGSEASTPRSPAPWC"
BASE COUNT          300 a          830 c          807 g          284 t
ORIGIN      
        1 cggtccttcc gtctcggacc agcggacctg ggcgacccga ggttccacga gcagtgtcgc
       61 ggcaacgccg gcgggtcgga tcagcgaagt cgccagtgtt cagcccgggt cgcgggcggt
      121 cccgccgggc gtgccggtga cgaccaggtc acccggatcc agccgctgga accgggccag
      181 ccgcgtcagc accacggtgc tggggatctc ggcgtcgatg cctcggaccc gccggcagcc
      241 gcggcgccgg tccgcccgct cggccctcgc ctcccgatcc agcgggcgcc tcgggtccgc
      301 gggctgtgga gcggcacgcc accacggatc ccgtcgccgc cggatcgctg cgcgggcgcc
      361 ggaggcgcgg catgcgaatg ctcggccttg atgcgctcgg cccgctccag ctccagggtc
      421 cggagggtcc gctcgatgcc ggcgagctgt ggggcggtgg cttgagggag gcggtaggcg
      481 tcgccgacga acccggccat gtcggcgcgg tagtcgtggc cgaacgaggt gaactcgccc
      541 ggcaccttca gcatcgcgtt catcgcgtcc atcccggtct ggaggaaggt gaccaggggc
      601 agccagcgca tctgctcggg caccccgcgg cctcgctgcc cgtcggccag ccaccagggc
      661 cgctgcacca gcaggtcggg cccgaacacg gcgatggggt cgttgtcgtg ggagagcacc
      721 accgcccgta gccgggcccg ttcctcatcg gccagctccg ccagctgctc ctggcggtcg
      781 aacacctcga cggtcccgtg cgggacgagg gtgctgctgc cccgggtcat gccgctccgg
      841 gaccacttgg ccagccacgg caggccgacc cacagggcac ggtcgatccc gtagtggtcg
      901 aagccgtcga tgccctggta catgagcacg tcggagctgg tccaggcacc catgctctcg
      961 ccgaagacca gcacccgcgg gcgcccttcg ggtggcaggc cggccagccg ctgcttgacc
     1021 ccccacagca ggagccggaa ctgccggcgg cccagcgcca ccttctggac cgagaggaac
     1081 gacgggtacc gggcgtactg gatgcagcag gtcgcgatgt cgccgcgggt gaggaactcg
     1141 gcggcctcga tcagcgtctg gtcgacccag ccggtgccgg tcggtgagat cagcagcagg
     1201 taggaccggt cgaacgcgcc ggcgcggtcc agctcggcca gggccagctc cgcccggccg
     1261 gtctggtaca gcggctcgct gttgaacccg acataggtgc ggatcggctg ggcgatggcc
     1321 ggctccccca tgacctcctc gatcagctcg ggggtgacca cgtcggtgac gaagcggcgt
     1381 ccctgctggc cgaggtcggc gaacggcagc aggctctcgg ggcaccccga caccagcggg
     1441 ctggcgggcg cggtggcata gcccggctcc accttctggt tggcccgccc cacataggcc
     1501 actccggcgt tgtagaccgc catcgtcgcc cctgcccaca gtccggcgtt gaccagccgc
     1561 gcgagcaccc gcttggacgg cccgggcccg aagtaggact cgagcgcgcc acgtgaccag
     1621 acgaagcctc tggccaggcc ggtcccgacc gtcgtgacgg catacgaggt ggccagcgcg
     1681 cccggcagcg tggccgtctc cggccgcggc cagcggtcca ctccggcctc ccgagcgggc
     1741 agccgccgac ctgcccggta gagcagtcct gcggtgtgga tggcggcgac ggcgatcggc
     1801 tggacagccc gctggctcgg gtagcgccgc tgcacccagc ggccaagatc gtggacggcg
     1861 gcaccggcgg caccgtcccg gagcagctga ccggtggacc gcagcgacgc cacccacaac
     1921 cgctggcagt ggcgctccgg aaccgccgcc agcgccgccc cggcgccgcc gatcaccgcc
     1981 cgggccgcga gctggtgggg aaggggggcg tcggccggca cggccagcct cgtcagcttc
     2041 tcgaccacgc cggtcgttgc ccgcgccccg agcatcccga gccccatcgc gacaccgtgc
     2101 agcctcgcgg tgcgcggcat cagcgacggc ccgaatgcgt cgagggcggc cacggtctgc
     2161 cggggcgccg ccggtgatgc cgtcaccggg ttcagccacc gtgccagctc ctgcagctgg
     2221 t
//
LOCUS       DASSWR010000457         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1118866, whole genome shotgun sequence.
ACCESSION   DASSWR010000457 DASSWR010000000
VERSION     DASSWR010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118866"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFO47_07650"
     CDS             complement(<1..616)
                     /locus_tag="VFO47_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788606.1"
                     /translation="MRTRADAGGPDVLGRVRTIWRVALGGLWLAVRALAARAGLTLAL
                     TALTAFAVGVAAAGAMYLRAAGESLLQDRLAATSPFSAGLLVERKVTTSTEVARLQHL
                     LRHDPRLAVPALKPPLFGLESEGFLTYSRPGDSDGPRARIAAREGLCEHLRLRAGRCP
                     AAGAQGQRMQAAVSPRMARRLGLRPGSRLEATTLRGEPVVPGLVV"
     gene            complement(715..1014)
                     /locus_tag="VFO47_07655"
     CDS             complement(715..1014)
                     /locus_tag="VFO47_07655"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HET8788607.1"
                     /translation="MSAGAGPRLAEVDAALAGGTALPRDNGELVFEEPWQGRALGMGV
                     VVLDRTGASWAEFRRHLVAAIRQRPPEPGESAASAYYAAWLDALEALLAERGLLP"
     gene            complement(1011..1631)
                     /gene="nthA"
                     /locus_tag="VFO47_07660"
     CDS             complement(1011..1631)
                     /gene="nthA"
                     /locus_tag="VFO47_07660"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET8788608.1"
                     /translation="MSTEHGHDHHHPRPDSRAALRARALEALLAEKGLVAADAIDAVV
                     ELYERDVGPQNGARVVARAWTDPAFRERLLGDGAKAIAELGFGGAEADTLVAVANTPT
                     VHNVVVCTLCSCYPWPVLGLPPTWYKSPPYRARMVAEPRAVLREFGLELPDEVEIRVW
                     DSSAEVRYLVVPMRPAGTDGLTEEALGALITRDCMIGTAVPQAGSP"
     gene            complement(1628..2299)
                     /gene="nthB"
                     /locus_tag="VFO47_07665"
     CDS             complement(1628..2299)
                     /gene="nthB"
                     /locus_tag="VFO47_07665"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HET8788609.1"
                     /translation="MDGIHDLGGMHGFGPVVVPGGEAPYHERWEGRVFALHLLVKLER
                     LGARPGGRATREEMDPADYLAASYYERWLWSAEQGLLRKGTIAAGEVEELMGRLAAGG
                     PEPPPHRDPEQAARAVMWLQEVVPMQPATRSRFVPGQGVRVRRMRPAGHTRCPRYVRG
                     ALGVVERVRGLGELPDLAVYSKDAPVEPVYSVAFRSDELWGRGEEPPWTVALDLWESY
                     LEEAE"
     gene            complement(2488..3231)
                     /locus_tag="VFO47_07670"
     CDS             complement(2488..3231)
                     /locus_tag="VFO47_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET8788610.1"
                     /translation="MIEVVPLRLGTIDVDVADLRFGHTGMDTLAVPMWAWLVRSGDEA
                     ILVDAGPPSLEWCAGNTLPVRDATERQLHEALARHGVAPRAVRTVVLTHLHWDHCGGL
                     GLFEHAQVLIQQRERGYAADPLWVHERPFRYAHEFLLHQSPLSAAVPLDGDTSVAPGI
                     RILAAPGHTPGSQVVVVGAGSGSAILAGDTISIEANRARSDGRIHPGGVFVRLDEYVE
                     TLARLTRLPGKILPGHEPSITADVPIAMH"
BASE COUNT          473 a         1252 c         1199 g          428 t
ORIGIN      
        1 tcaccacaag gccgggaacg accggctcgc ctcgcagggt ggtggcctcc aggcggctcc
       61 ccggccgcag gccgagccgc cgcgccatgc gcgggctcac cgcggcctgc atccgctggc
      121 cttgcgcgcc ggccgccggg cagcggccgg cgcgcagccg caggtgctcg cagaggccct
      181 cgcgggcggc gatccgcgcc ctcggtccgt cgctgtcccc aggccgcgag tacgtcagaa
      241 agccctcgct ctccagcccg aacaacggcg gtttcagcgc gggcacggcc agcctgggat
      301 cgtgccgcag caggtgctgg agccgcgcca cctcggtgct ggtcgtgacc ttccgctcga
      361 ccagcagccc ggcgctgaat ggcgaggtcg ctgcgagccg gtcctgcagc agggactcgc
      421 cggccgcccg caggtacatg gccccggcgg cggccacgcc gaccgcgaac gcggtcaggg
      481 cggtcaacgc cagcgtcagc ccagcccggg cggccagggc gcgcacggcc agccacaggc
      541 caccgagcgc gacccgccag atcgtccgca cccgccccag gacatcaggc ccgcctgcat
      601 ccgcgcgcgt ccgcatggtc cacaccgtag cgagcaagcc atgctcagga aagcccggac
      661 atgcgctgta tcacctgaag gggtcgacgt accggagcgg gccgtgcctg ccggtcaggg
      721 gagcaggccg cgctcggcca gcagcgcttc gagggcgtcc agccaggcgg cgtagtaggc
      781 gctggcggcc gactcgcccg gctccggcgg gcgctgccgg atcgcggcca ccaggtggcg
      841 gcggaactcc gcccagctcg cgccggtacg gtcgagcact accacgccca tgccgagggc
      901 gcgtccctgc cagggttcct caaagaccaa ctcgccgttg tcgcggggca gcgccgtgcc
      961 cccggcgagc gcggcgtcca cctcggcgag ccgtggcccg gcgccggcgc tcacggcgac
     1021 cccgcctgcg gcacggccgt gccgatcatg cagtcacggg tgatcagcgc acccagggcc
     1081 tcctcggtca ggccatcggt gccggcgggc cgcatcggta cgaccaggta gcggacctcg
     1141 gcgctggaat cccagacccg gatctcgacc tcgtcgggca gctccaggcc gaactcgcgc
     1201 agcaccgcac gcggctcggc caccatgcgg gcacggtacg gcgggctctt gtaccaggtg
     1261 ggcggcaggc cgagcacggg ccacgggtag caggagcaga gcgtgcacac gaccacgtta
     1321 tggacggtcg gggtgttggc gaccgcgacc agggtgtcgg cctcggcgcc gccgaagccc
     1381 agctcggcga tggccttggc gccgtccccg agcagccgct cgcggaaggc ggggtcggtc
     1441 caggcgcgcg ccaccaccct ggcgccgttc tgcgggccga cgtcgcgttc gtagagctcc
     1501 acgaccgcgt cgatagcgtc ggcggcgacc aggcccttct cggccagcag cgcctccagg
     1561 gcgcgggcgc gcagggccgc ccgcgagtcc gggcgggggt ggtgatggtc gtgcccgtgc
     1621 tcggtgctca ctcggcctcc tcgaggtagc tctcccacag gtcgagcgcc accgtccacg
     1681 gtggttcctc gccccggccc cacagctcat cggagcggaa ggcgaccgag tagacgggct
     1741 cgaccggcgc gtccttgctg tagacggcca ggtcgggcag ctcccccaag ccccggacgc
     1801 gctccaccac acccagggcg ccgcgcacgt agcgggggca gcgcgtgtgg ccggccgggc
     1861 gcatgcggcg cacccgcacg ccctgcccgg gcacaaagcg cgaccgcgtc gccggctgca
     1921 tcgggacgac ctcctgcagc cacatgacgg ctcgtgcggc ctgctccggg tcccggtgcg
     1981 ggggcggttc cggcccgccg gcggccagcc gccccatcag ctcctcgacc tcgccggccg
     2041 cgatcgtccc cttgcgcagc agcccctgct cggcgctcca cagccagcgc tcgtaatagg
     2101 aggccgccag atagtcggcc gggtccatct cctcacgggt cgcgcggccg cccggcctcg
     2161 cgcccagacg ttccagcttc accagcaggt ggagggcgaa gacgcgcccc tcccaacgct
     2221 cgtgatacgg cgcctcgcca ccaggcacga ccaccgggcc gaacccgtgc atgccgccca
     2281 gatcgtggat gccgtccatc taccgccaaa tataacccgg gcccagtgac cgggctgcgc
     2341 agccgccgat catgccggcg ggaatgtggc cggggtggcg gcagtgtctc tatccatcct
     2401 cgtgtgccct ccgtccccgt tggccctgat gcctgcgcag gcggagatgg cggactcatc
     2461 gcgcacctga ctagttcagc tgggcgatca gtgcatggcg atggggacgt cggccgtgat
     2521 ggagggctcg tggccgggga ggatcttgcc cggcaggcgg gtgaggcggg caagggtctc
     2581 gacgtactcg tcgaggcgga cgaagacgcc gcccgggtgg atgcggccgt ctgagcgggc
     2641 ccggttggcc tcgatgctga tcgtgtcgcc ggcaaggatg gcactgccgc tgcctgcgcc
     2701 gacgacgacc acctgcgatc ccggggtatg gccgggggcg gccagaatcc ggatgcccgg
     2761 cgcaacactg gtgtcgccgt cgagcgggac ggcagccgac agcgggctct ggtggagcag
     2821 gaactcgtgc gcgtacctga acggccgctc atgcacccac agcgggtcgg cggcatagcc
     2881 gcgctcgcgt tgctggataa ggacctgcgc atgctcgaac agaccgaggc cgccgcagtg
     2941 gtcccagtgc aggtgggtca ggaccaccgt gcgcaccgcg cgcggcgcaa cgccgtgtcg
     3001 tgcgagcgcc tcgtgcagct ggcgctcggt cgcatcacgc accggcaggg tgttgccggc
     3061 gcaccactcc aggctcggcg gaccagcatc gacaaggatc gcctcgtcgc cggagcgcac
     3121 cagccatgcc cacatcggca cggcgagcgt gtccatgccg gtgtgcccga agcgcaggtc
     3181 ggccacgtcg acgtcgatgg tgcccagacg caacgggacg acctcgatca tcgctggacc
     3241 ctctcagcgc agcgccgact gctcacagag atggatcgag accccgatgg ccgggcgcat
     3301 cactgctggc cttcgccctg gtcttgaggg tacagctccg ggtgcttgta gc
//
LOCUS       DASSWR010000458         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_780185,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000458 DASSWR010000000
VERSION     DASSWR010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780185"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..364
                     /locus_tag="VFO47_07675"
     CDS             224..364
                     /locus_tag="VFO47_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788611.1"
                     /translation="MEAPTSRRAFFRVAGRSLAAATAAVLLAGCPGGDDDDDDGGGDD
                     DD"
     gene            complement(377..1228)
                     /locus_tag="VFO47_07680"
     CDS             complement(377..1228)
                     /locus_tag="VFO47_07680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8788612.1"
                     /translation="MPVPDARRNGAVQLVVTDLDGTLSDADERIHPASVRAVRELEAA
                     GIPVLVATGRRLRMTLAVLEAGGLTGPAVVLDGALGLDLRDGRVFHQVAFPPAAAVRA
                     LAAFTTAGLSPCVYVDRPGVDLVVGDHPSTNPGHLARARPWVAVDDLDRVVTDEPVYT
                     FAVVGRPAALLEPVLREVGAAGSASIVPDLIYGGFTLQVRPPDISKWSGVLAYCAEQG
                     IDPARVLAVGDGANDLELLDAAAVACAVATATPDVLARADHVIGPPSSGGWAAVLDLV
                     GRPGAPG"
     gene            complement(1215..>2033)
                     /locus_tag="VFO47_07685"
     CDS             complement(1215..>2033)
                     /locus_tag="VFO47_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8788613.1"
                     /translation="TAPGTPPRLTVLDVVPEGTSEGPVATGLVERIRALERPVAAGVT
                     GPAAFLVDYRDSLTSRLPYALGLIGLATFALLFLMTGSVVVPVKAIVMNVLSLGASFG
                     ALVWVFQDGHLSGLLGFDSPGMVDITVPVLIFVFAFGLSMDYEVFLLSRIKEAWDQTG
                     DNDRAVALGLQRTGRIVTSAAALIVIVFLGFAAGELLTIKEVGLGMAIAVVLDATVVR
                     MLLVPATMKLMGRWNWWAPPSLRRLHDRFGLAEQALPPYRAAEQERVGSLHAGS"
BASE COUNT          298 a          741 c          766 g          228 t
ORIGIN      
        1 cgatcacgcc ccgctggccc ggcggccggt ccggctcgaa gtggccctcg gccagctcct
       61 ccttgatctc gaagggcacg tcggccacgc attccggctg ctgctccaga tcaaaggcca
      121 tggcccgccc cctcgcatcc atccgagcac tatgcactat atagatctga gtgcgatgtt
      181 agggtgcgtt tcgcagcggg gggaccgagg aggaggtctg accatggaag cgcccacgag
      241 ccgccgcgcc ttcttccggg tcgccggccg ctccctcgcc gcggccaccg cggccgtgct
      301 cctggccggc tgcccgggcg gcgacgacga tgacgacgac ggcggcggcg acgacgacga
      361 ctgaggaccc ggggcgtcag ccgggggcgc cggggcggcc gacaaggtcg aggacggcgg
      421 cccagccgcc gctggacggg gggccgatga cgtggtcggc ccgggccagg acgtccgggg
      481 tggcggtggc gacggcgcag gccacggcgg cggcgtcgag cagctcgagg tcgttggcgc
      541 cgtcgccgac ggccaggacg cgggccgggt cgatgccctg ctcggcgcag taggccagga
      601 ccccgctcca cttgctgatg tccgggggtc gtacctggag ggtgaagccg ccgtagatca
      661 ggtcggggac gatgctggcg ctgccggccg ccccgacctc ccggaggacc ggctcgagca
      721 gggccgcggg ccggccgacc accgcgaacg tgtagacggg ctcgtcggtg accacgcggt
      781 ccaggtcgtc gacggccacc cagggccggg cccgggccag gtggccgggg ttggtcgagg
      841 ggtggtcgcc gacgaccagg tccaccccgg gccggtcgac gtagacgcac gggctcaggc
      901 cggcggtggt gaacgccgcc agcgcccgca cggcggccgc gggcgggaac gccacctggt
      961 ggaacacccg gccgtcgcgc aggtcgagcc cgagggcgcc gtcgagcacc accgccggcc
     1021 cggtcaggcc gcccgcctcc agcaccgcca aggtcatgcg cagccgccgg ccggtggcca
     1081 ccagcaccgg gatcccggcc gcctccagct cgcggaccgc ccgcaccgag gccgggtgga
     1141 tgcgctcgtc ggcgtcggac agggtgccgt ccaggtcggt gacgaccagc tggaccgcgc
     1201 cgttgcgccg cgcgtcagga accggcatgg agcgagccta cccgctcctg ctcggcggcc
     1261 cggtagggcg ggagcgcctg ctcggccagc ccgaagcggt cgtggagccg acgcagggac
     1321 ggcggggccc accagttcca gcggcccatc agcttcatgg tcgccgggac cagcagcatc
     1381 ctgaccacgg tggcgtcgag cacgacggcg atggccatgc ccaggccgac ctccttgatg
     1441 gtcagcagct ccccggccgc gaagccgagg aacacgatca cgatcagggc cgccgccgag
     1501 gtcacgatcc gcccggtgcg ctgcagcccg agggcgaccg cccggtcgtt gtcgccggtc
     1561 tggtcccagg cctccttgat ccgcgagagc aggaacacct cgtagtccat ggacaggccg
     1621 aaggcgaaca cgaagatcag caccgggacg gtgatgtcga ccatgccggg ggagtcgaag
     1681 cccagcagcc ccgacaggtg gccgtcctgg aacacccaga ccagggcgcc gaagctggcc
     1741 cccagcgaca gcacgttcat gacgatcgcc ttgaccggca ccaccaccga cccggtcatc
     1801 aggaacagca gggcgaaggt ggccagcccg atcagcccga gcgcgtacgg cagccggctg
     1861 gtcagcgagt cgcggtagtc gaccaggaag gcggccgggc cggtgacccc ggccgcgacc
     1921 ggccgttcca gggcgcggat ccgctcgacc aggccggtcg ccaccggccc ctcgctggtc
     1981 ccctcgggga cgacgtcgag cacggtgagc cgcggcgggg tgccgggggc ggt
//
LOCUS       DASSWR010000459         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_695713,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000459 DASSWR010000000
VERSION     DASSWR010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695713"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFO47_07690"
     CDS             <1..759
                     /locus_tag="VFO47_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8788614.1"
                     /translation="PVVKDGYVYLEGTLTSAPDSQAGYATVDRLRASVHAIGGADAKV
                     GGGSAVNLDIQRATRHDRNLVVPLVLLVVLVILALVLRAIVAPLLLVATVVLSFGAAL
                     GVSALVFDHVFGFAGSDPAFPLWTFVFLVALGTDYNIFLMTRVHEESKRYGTRRGALI
                     GLAATGGVITSAGIVLAGTFAALGTLPLVFITEIGFAVAFGVLLDTFVVRSVLVTALN
                     LDVGRWIWWPSSLHRKRDDVDLGQLEAEAAEVSR"
     gene            complement(1092..1499)
                     /locus_tag="VFO47_07695"
     CDS             complement(1092..1499)
                     /locus_tag="VFO47_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788615.1"
                     /translation="MLKRLMYVTLYVSDQDRALEFYTERLGLQKRADNSNPEGRFLTV
                     APADDSMEILLWPGNPAQGPVDATEPGVVAGPVFLRSDDLRKDFEEFRSRGVEFVEPE
                     PVDYAYGIRITALDPDGNRLELRQRPRGWSKER"
     gene            1603..>2034
                     /locus_tag="VFO47_07700"
     CDS             1603..>2034
                     /locus_tag="VFO47_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8788616.1"
                     /translation="MDDQQWLTERFQQHRPHLRAVAYRMLGSLTDADDAVQDAWERVS
                     RARADEVGNLKAWLTTIVARVCLNLLRSRNAQREESLEVSVPDPIVTLEGGPDPEQQV
                     LLADSVGLALQVVIDTLAPAERLAFVLHDLFDLPFEEIAPMV"
BASE COUNT          295 a          698 c          712 g          329 t
ORIGIN      
        1 ccggtggtca aggacggcta tgtgtacctg gagggcacgc tgaccagcgc accggacagt
       61 caagccggtt atgccaccgt ggaccggctc cgggccagcg tgcacgcaat cggtggcgcc
      121 gacgccaagg tcggcggggg cagcgccgtc aacctggaca tccagcgggc tacccgccac
      181 gaccgcaacc tggtggtgcc gctggtcctt ttggtggtgc tggtcatcct cgccctggtg
      241 ctgcgcgcga tcgtcgcgcc gctgctgctg gtcgccaccg tggtgctgtc gttcggcgcg
      301 gccctggggg tgagtgccct ggtcttcgac cacgtcttcg ggtttgccgg gtccgacccg
      361 gcgttcccgc tgtggacctt tgtgttcctg gtcgcgttgg gcaccgacta caacatcttc
      421 ctcatgacca gggtccacga ggagtccaag cgctacggga cccggcgggg ggcgctgatc
      481 ggcctggccg ccaccggcgg ggtgatcacc tcggcgggga tcgtgctggc cgggaccttc
      541 gccgccctgg ggacgctgcc gctggtgttc atcactgaga tcggcttcgc ggtcgccttc
      601 ggggtgttgt tggacacctt cgtggtccgc tcggtcctgg tcaccgccct gaacctcgac
      661 gtcggccgct ggatctggtg gccgagcagc ctgcaccgca agcgcgacga cgtcgacctg
      721 ggccagctcg aagcggaggc ggcggaggtc agccgctgac tcttggcgcc gacggcgcgg
      781 gacgatcagg accggaagac gggtccaggc cggcgagctc ggccagcggg gcggccaccg
      841 cacaggcgaa gccgagtgcg gccaacagcc gtgcccacca ctgcggggac cgggtgggtc
      901 ccggtggcgg gggacggtgg cgtcgccact gcaccggatc tggaggaggg tcggcaggcc
      961 ggccaccagc agctaggcgg cgatcaggac cagggcaagg acggccatcg gcggcacccc
     1021 tgtgttcggg gatgggacgc ggcttccagc cggaccctcc atgggctcgg ctgggccgct
     1081 tgggtgggca gctatcgctc cttggaccag cctcggggcc gctgacgcag ctcgagccgg
     1141 ttgccgtcgg ggtccagcgc cgtgatgcgg ataccgtagg catagtccac gggctccggt
     1201 tccacgaact ccacgccgcg ggaccggaat tcctcgaagt ccttgcgcag gtcgtcggac
     1261 cggaggaaga ccggaccggc cacgacgccc ggctccgtcg catcgaccgg accctgggcc
     1321 gggttgcccg gccacagcag gatctccatc gagtcatcgg ccggcgcgac ggtcaggaag
     1381 cgtccttcag gattggagtt atcggcacgc ttctgcagtc cgagacgctc ggtgtagaac
     1441 tccagcgccc ggtcctggtc gctcacatag agcgtcacat acatcagtcg cttcaacatc
     1501 acgtcctcct cattggttcg ggcacctccg cgctggtcct gtcacatcga ccgggtttgg
     1561 cgggtcacta ctgatgacac gccaagacga ggaaacgtga ccgtggacga ccagcagtgg
     1621 ctgaccgaac ggttccaaca gcaccgaccc cacctgcggg cggtggccta ccggatgctg
     1681 ggctcactca ccgacgccga cgacgccgtc caggacgcct gggagcgcgt cagccgcgca
     1741 cgggccgacg aggtcggcaa cctcaaggcg tggctgacca cgatcgtcgc ccgggtctgt
     1801 ctcaacctgc tgcgctcgcg caacgcccag cgcgaggagt ccctggaggt gagcgtccct
     1861 gatccgatcg tgaccctgga gggcgggccc gatcccgaac agcaggtgct gctggccgac
     1921 tcggtcggcc tcgccctgca ggtcgtgatc gacaccctgg cccccgccga gcgcctcgcg
     1981 ttcgtcctcc acgacctgtt cgacctgccc ttcgaggaga tcgcgccgat ggtc
//
LOCUS       DASSWR010000460         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_300055,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000460 DASSWR010000000
VERSION     DASSWR010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300055"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..791
                     /locus_tag="VFO47_07705"
     CDS             30..791
                     /locus_tag="VFO47_07705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8788617.1"
                     /translation="MADHPPKIAVVTGAGSGIGAAVAHALAGEGWVVVLAGRRRDALE
                     SVAERGRDLPGVLDPVPADVTDEPSVRALFDRVVERHGRVDLLFNNAGTGAPARELDA
                     VPLAEWDAVVAVNLTGAFLCTREAFRVMRRQRPRGGRIINNGSVSAHAPRPRSVAYTA
                     TKHAITGLTRSTALDGRPYDIACGQIDIGNAATGMTEPMAAGVLQADGSVRPEPRMDV
                     ADVARAVLYMAGLPLDANVAAMTVMATRMPFVGRG"
     gene            1178..1933
                     /locus_tag="VFO47_07710"
     CDS             1178..1933
                     /locus_tag="VFO47_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8788618.1"
                     /translation="MDGADPDRWSEVAAGWSELWGEFARPAWGAVVAATGIGQGSRVL
                     DVGCGSGEFLAHLARLGAWPAGIDPAPGMVELARSRVPGADVRLGPAQPLPWPDGSFD
                     VVTAVNALQFAGDTLDALAELVRVTVPGGQVAVANWAEGDRNDLDTIEAAVAAAADEE
                     PLPDGDLRQPGGLERLLADAGLADVAAGLVEVPWEAPDDAALVGGVLLGEDPATTSAR
                     APAVLAAARPFRTPTGGYRLVNAFRYAAGRTPG"
     gene            complement(1948..>2127)
                     /locus_tag="VFO47_07715"
     CDS             complement(1948..>2127)
                     /locus_tag="VFO47_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788619.1"
                     /translation="QRDCDPSYPDVCLDPDAGDYDCAGGSGNGPEYVEGPIRVRPPDP
                     FDLDREGDGWGCESG"
BASE COUNT          247 a          859 c          770 g          251 t
ORIGIN      
        1 gcatgctccc acaccgacct ggaggaccga tggccgacca cccgcccaag atcgcggtcg
       61 tcaccggggc cgggagcggc atcggcgcgg cggtcgcgca cgccctggcc ggcgaaggct
      121 gggtcgtggt cctggccggc cgccgccgcg acgccctcga gtcggtcgcc gagcggggcc
      181 gcgatctgcc cggcgtcctc gacccggtcc cggccgacgt caccgacgag ccgtcggtgc
      241 gggcgctgtt cgaccgggtg gtcgagcggc acggccgcgt cgacctgctg ttcaacaacg
      301 ccggcacggg ggcgccggcc cgcgagctcg acgccgtccc cctggccgag tgggacgccg
      361 tggtcgccgt caacctcacc ggcgcgttcc tctgcacccg cgaggcgttc cgggtgatgc
      421 ggcgccagcg gccccggggt ggccggatca tcaacaacgg ctccgtctcc gcccacgcgc
      481 cacggccgcg ctcggtcgcc tacaccgcca ccaagcacgc catcacgggg ctgaccaggt
      541 cgaccgcgct cgacgggcgt ccctacgaca tcgcctgcgg ccagatcgac atcggcaacg
      601 ccgccaccgg tatgaccgag cccatggcgg ccggcgtcct ccaggccgac ggcagcgtcc
      661 gccccgaacc ccggatggac gtggccgacg tggcccgcgc cgtcctgtac atggccggcc
      721 tgcccctgga cgccaacgtc gccgccatga ccgtcatggc gaccaggatg ccgttcgtgg
      781 gtcgcggttg agccgaacgc cggccgcagc cagatggcgt cgaccgcttg accttggagc
      841 acgctccagg gtctaccgtg cggccatggc accgaaggga ggtgaccgcc atgccgctgc
      901 gggacgaccc gctcgactgc ccctgcgacg actgcccacc cggctgctgc tcgctgggct
      961 gatcagcccc aaggccgcgg gaccggccgg cgccaccggt ctcgcgcccg cttcccccgc
     1021 ccggcccgcc aggctaaggt cggcagggcc gaggggatca ggggaagggg cgagcgtggg
     1081 tgatcgtcgg gtgggcctcc gccggggccg tcacgccggc tggtgggtcg agggccggtc
     1141 gggcccggcc gaggtcagcg acggcgacac gccctgaatg gacggagccg accccgaccg
     1201 ctggtccgag gtcgccgccg gctggtccga gctgtggggc gagttcgccc gccccgcctg
     1261 gggggccgtg gtcgcggcca ccggcatcgg ccagggctcg cgggtgctgg acgtgggctg
     1321 cggcagcggc gagttcctgg cccacctggc ccggctcggc gcgtggccgg cgggcatcga
     1381 cccggccccc gggatggtcg agctcgcccg ctcccgcgtc cccggcgccg acgtccgcct
     1441 cggccccgcc cagcccctcc cctggccgga cgggagcttc gacgtggtca cggccgtcaa
     1501 cgccctgcag ttcgccggcg acaccctgga cgccctcgcc gagctcgtcc gcgtcaccgt
     1561 gcccggcggg caggtggccg tcgccaactg ggccgagggc gaccgcaacg atctcgacac
     1621 aatcgaggcc gccgtggccg cggccgccga cgaggagccc ctgcccgacg gcgacctccg
     1681 ccagcccggc ggcctggagc ggctcctcgc cgacgccggc ctcgccgacg tggccgcggg
     1741 cctggtcgag gtcccctggg aagcccccga cgacgccgcc ctcgtcggcg gcgtcctcct
     1801 gggcgaggac cccgccacca cctccgcccg ggcgccggcc gtgctcgccg ccgcccgccc
     1861 gttccgcacc cccaccggcg gctaccggct ggtcaacgcc ttccgctacg ccgccggccg
     1921 taccccgggc tgaccgcaag gcgtcgctca gccggattcg cagccccagc cgtcgccctc
     1981 ccggtccagg tcgaacgggt ccggggggcg cacgcggatg gggccctcga cgtactcggg
     2041 cccgttgccc gaaccgcccg cgcagtcgta gtccccggcg tcggggtcga ggcagacgtc
     2101 gggataggac gggtcacagt cccgctg
//
LOCUS       DASSWR010000461         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_610636,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000461 DASSWR010000000
VERSION     DASSWR010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610636"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1035)
                     /locus_tag="VFO47_07720"
     CDS             complement(88..1035)
                     /locus_tag="VFO47_07720"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET8788620.1"
                     /translation="MTFDCSVAVVAGGLSFEREVSLRSGQRVADALRERGYRVGELDA
                     DHELVEKLANDAFDVAFLALHGRFGEDGTVASILELLGIPYTGSSFAASRLAFDKLAA
                     KSVLRRAGLSVPAAIPLTEGALRELGVGSLLDRAMDELGLPIVVKPNRGGSALGIRLV
                     ERPDQLPAALMAAFGYDDTVLLEQRVLGTELALSVVDGLATLPAVEIRPKEGWYDFSA
                     RYTHGASEFRVPAGLDPATTEQCLRAAHAAHLALGCRDISRVDAILDPEGTCRILEVN
                     TGPGLTTTSLVPMAADAAGLTFADLAAHLTDRATARRPR"
     gene            complement(1032..2324)
                     /locus_tag="VFO47_07725"
     CDS             complement(1032..2324)
                     /locus_tag="VFO47_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET8788621.1"
                     /translation="MRADDPFLDRYARGTRSMTASEIRALFAVASRPEIVSLAGGMPF
                     TAALPFDAVGDVVGEVLADEGPAALQYAGGQGDPRLREVLTELMRHEGIHGSGGDVVV
                     TEGSQQALDLVSRMFCDPGDVIVAEGPSYVGALSAFSQYQVDVCHVPLDDDGLDPDAL
                     AVTLERLERDGRRVKLLYTVPNFHNPAGVTMAAGRRERVLELARRHDLLVLEDNPYGM
                     LGFESDPPDALRSLDPENVVYLGSLSKVFCPGLRIGWALVPPALRERLVLVKEAADLC
                     SSSFTQAVAYRWFANSPWRETLKGLREVYRERRDVMLEALAEDVPAGVTWTRPAGGFY
                     VWVRLPDHLDARAMLAKAITARVAYVPGGAFYADGQGSSTMRLSYCFPPPDRIREGVR
                     RLAGVIDEELDLVRALGGLPLGDAETDQGRPAGWGGVQ"
     gene            2540..3175
                     /locus_tag="VFO47_07730"
     CDS             2540..3175
                     /locus_tag="VFO47_07730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788622.1"
                     /translation="MGRKLVPLGPETLDLLPAPCRRCVFWELGARARARGSEDAALQK
                     EAWLSSVALEWGPAGHVVTVDGKVAGYSLYAPTHVLDQVGFQAARPVSRDAVLLATLA
                     VLPEFEGQGIGRVLLQTMARDLTKRKVRAVEAFADRAPAGRDCRVPAGFLEASGFQVV
                     RDHPRYPLMRMDLRTALTWKDAREAALEALRVPAPAQPALRPVPSSSRPPA"
     gene            complement(3242..3568)
                     /gene="trxA"
                     /locus_tag="VFO47_07735"
     CDS             complement(3242..3568)
                     /gene="trxA"
                     /locus_tag="VFO47_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET8788623.1"
                     /translation="MTKAAAVTDATFDSEVLKSSTPVLVDFWAEWCGPCKMISPVLEE
                     IADEHKGKLTIAKLNVDENPDVAMRYGVMSIPTLALFIGGVEKKRLIGAMPKRNIVSE
                     LSEFIS"
     gene            complement(3698..>4473)
                     /locus_tag="VFO47_07740"
     CDS             complement(3698..>4473)
                     /locus_tag="VFO47_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8788624.1"
                     /translation="MGKMREQAERFGAEFIRADATKVELAGPPFRAWVEDVEYTGKAL
                     IIATGAKARMLGLPGERQYLGRGVSTCATCDGFFYRSRELVVVGGGDSALEEATFLTR
                     FATKVTVVHRRDRLRASKVMQDRAFANPKIDFEWNSIVTDVLGEQTVNGVRLRNVEDD
                     TEKVLPVAGVFVAIGHTPNTDLFGGQVELDQAGYILTPNPPSTATSVDGVFAAGDVVD
                     HTYRQAVTAAGTGCAAAIDAERWLEAHGHDSGATTDNWN"
BASE COUNT          630 a         1522 c         1687 g          634 t
ORIGIN      
        1 aacgccggag tcagccgaac gccggagtca gccgaacgcc ggagtcagcg gaacgccgat
       61 cagcgacgtg ccgatcgggg acgaacctca gcgggggcgg cgggcggtgg cgcggtcggt
      121 gaggtgggcg gcgaggtcgg cgaaggtgag gccggcggcg tcggcggcca tggggaccag
      181 gctggtggtg gtgaggccgg ggccggtgtt gacctcgagg atgcggcagg tgccttcggg
      241 gtcgaggatg gcgtcgaccc gggagatgtc gcggcagccg agggcgaggt gggcggcgtg
      301 ggcggcccgg aggcactgct cggtggtggc ggggtcgagg ccggcgggga cccggaactc
      361 ggaggcgccg tgggtgtagc gggcggagaa gtcgtaccag ccctccttgg gccggatctc
      421 gaccgcgggc agggtggcga ggccgtcgac gaccgagagc gccagctcgg tgccgaggac
      481 ccgctgctcc aggaggacgg tgtcgtcgta gccgaaggcg gccatgagcg cggccgggag
      541 ctggtcgggg cgctcgacca ggcggatgcc aagggccgag ccgccgcggt tgggcttgac
      601 cacgatcggg aggccgagct cgtccatggc ccggtcgagc aggctgccga cccccagctc
      661 ccgcaaggca ccctcggtca gggggatggc cgccgggacc gagaggccgg ctcggcggag
      721 gacggacttg gcggccagct tgtcgaaggc gagccggctg gcggcgaagc tcgagccggt
      781 gtagggaatc ccgagaagct ccagaatcga cgcgaccgtt ccgtcctccc cgaaccggcc
      841 gtgcagggcg aggaaggcga cgtcgaacgc gtcgttggcg agcttctcca ccagctcgtg
      901 gtctgcgtcc agctccccga cccggtagcc gcgctcgcgc agggcgtcgg cgacccgctg
      961 gcccgagcgg agcgagacct cgcgctcgaa cgacaggccg cccgcgacga ccgcgaccga
     1021 gcagtcgaag gtcattgcac gccaccccag ccggccgggc gaccctggtc ggtctccgcg
     1081 tcgccgagcg ggagcccacc gagcgcccgc accaggtcga gctcctcgtc gatcacgccg
     1141 gccaggcggc ggaccccctc ccggatgcgg tccggcggcg ggaagcagta ggacaggcgc
     1201 atggtcgagg acccctggcc gtcggcgtag aaggcgccgc cgggcacgta ggccacccgg
     1261 gcggtgatcg ccttggccag catggcccgg gcatccaggt gatcgggcag gcggacccaa
     1321 acgtagaagc cgcctgcggg cctggtccag gtcacgccgg cggggacgtc ctcggcgagg
     1381 gcctcgagca tcacgtcgcg ccgctcgcgg tagacctcgc gaagcccctt gagcgtctcc
     1441 cgccaggggg agttggcgaa ccaccggtag gccaccgcct gggtgaagct ggacgagcag
     1501 aggtcggccg cctccttgac caggaccagc cgctcacgca gggccggcgg caccagcgcc
     1561 cagccgatgc gcagcccggg gcagaacacc ttggacaggc tgccgagata gacgacgttc
     1621 tccgggtcaa gcgaccgcag cgcatccgga gggtcgctct cgaaccccag catcccgtac
     1681 ggattgtcct ccaggaccag gaggtcgtga cgccgggcga gctccaggac ccgctcccgg
     1741 cggccagccg ccatcgtcac cccggccggg ttgtggaagt tgggaacggt gtagaggagc
     1801 ttcacgcgac gaccatcacg ctcaaggcgt tcgagggtta ctgcgagtgc gtcgggatcg
     1861 aggccatcgt cgtccagcgg tacgtggcac acgtcgacct ggtactgcga gaaggcggag
     1921 agggcgccga cgtaggaggg cccctcggcc acgatcacgt cgcccgggtc gcagaacatg
     1981 cgggagacga ggtcgagcgc ctgctgtgag ccctcggtga cgaccacgtc gccgccggac
     2041 ccgtggatgc cctcgtggcg catcagctcg gtgaggacct cgcgcagccg cgggtccccc
     2101 tggccgccgg cgtactggag ggcggccggg ccctcgtccg ccagcacctc cccgaccacg
     2161 tcgccgacgg cgtcgaaggg cagcgcggcc gtgaagggca tgccgccggc caggctgacg
     2221 atctcggggc gggacgcgac ggcgaacagg gcccggatct cggaggcggt catcgagcgg
     2281 gtgccgcggg cgtagcggtc aaggaagggg tcatcggcgc gcatgaggtt gaaggatagc
     2341 ggcatctggc aacccccctc aggcggctgc tagcatcgag ccgctatccg gcctgcggaa
     2401 acgttcctgt gctacgagtt gccctgccac tgctccgccg cgttgggggg agtttcttcg
     2461 tgggcctgtt cctcggcctg atctgggcct tgctggtcat ccaccgccgg ccgccgcgcg
     2521 cctcaggggc tgcctgacct tgggccgcaa gctggtcccg ctcgggcccg agaccctcga
     2581 cctgctgccg gcgccgtgcc gccgctgcgt cttctgggag ctgggggcca gggcgcgggc
     2641 gcgcgggtcg gaggacgcgg cgctgcagaa ggaggcctgg ctgtcctcgg tggcgctgga
     2701 gtggggcccg gccggccacg tcgtcacggt ggacgggaag gtggccggct actccctgta
     2761 cgccccgacc cacgtgctgg accaggtcgg gttccaggcc gcccggccgg tgagccggga
     2821 cgccgtgctg ctcgccaccc tagccgtcct gccggagttc gagggccagg ggatcggccg
     2881 ggtcctgctc cagacgatgg cccgtgacct gaccaagcgg aaggtgcggg cggtggaggc
     2941 gttcgccgac cgtgcgccgg ccggacgtga ctgccgcgtg ccggccgggt tcctggaggc
     3001 cagcggattc caggtcgtcc gggaccatcc ccgctatccg ctcatgcgca tggacctgcg
     3061 gacggccctc acctggaagg acgccaggga ggcggccctg gaggccctcc gggtcccagc
     3121 ccccgcccag cccgccctgc gccccgtccc ctcctcctca cgcccgcccg cctgagacag
     3181 gggccatgga cgacacgagc ccgacccacc tgggtcgggc tcgttggctt gaggagctgg
     3241 gtcagctgat gaactcggag agctcgctga cgatgttgcg cttcggcatg gcgccgatca
     3301 gccgcttctt ctccacgccg ccgatgaaca gggcgagggt ggggatgctc atgacgccgt
     3361 agcgcatggc cacgtcgggg ttctcgtcga cgttgagctt ggcgatggtc agcttgccct
     3421 tgtgctcgtc ggcgatctcc tcgagcacgg ggctgatcat cttgcagggg ccgcaccact
     3481 cggcccagaa gtcgaccagc acgggcgtgc tcgacttgag cacctcgctg tcgaacgtgg
     3541 cgtcggtcac ggctgcggct ttcgtcatgt cgtcggtgcg gtcggggtca cggccctagg
     3601 cacctccacc tcctcgtgcg gatgggtccc ccggccaggg tccaacgacc actggctcgt
     3661 ctgtattcct gcccattgac ggcggtcgtc aaccgagtca gttccagttg tcggtggtgg
     3721 ccccggagtc gtgaccgtgg gcctccagcc agcgctcggc gtcgatcgcg gccgcacagc
     3781 cggtgccggc ggccgtcacc gcctgccggt aggtgtggtc gacgacgtcg cccgccgcga
     3841 acacgccgtc gacgctggtc gcggtcgagg gcgggttcgg ggtcaggatg tagccggcct
     3901 ggtcgagctc gacctggccg ccgaacaggt cggtgttcgg ggtgtggccg atggcgacga
     3961 agactccggc caccgggagc accttctcgg tgtcgtcctc gacgttgcgc agccggacac
     4021 cgttcaccgt ctgttcgccg agcacgtcgg tgacgatcga gttccactcg aagtcgatct
     4081 tggggttggc gaaggcccgg tcctgcatga ccttggaggc gcggagccgg tcacgccggt
     4141 ggacgacggt gaccttggtt gcgaacctgg tcaggaaggt ggcctcctcg agcgcggagt
     4201 caccgccgcc gaccaccacc agctcgcggc tccggtagaa gaaaccgtcg caggtggcgc
     4261 aggtcgagac cccgcggccg aggtactggc gctcgccggg cagcccgagc atcctggcct
     4321 tggcgccggt ggcgatgatc agggccttgc cggtgtactc cacgtcctcg acccaggccc
     4381 ggaacggcgg gccggccagc tcgaccttgg tggcgtcggc gcggatgaac tcggccccga
     4441 agcgctcggc ctgctcgcgc atcttgccca tca
//
LOCUS       DASSWR010000462         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_639613,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000462 DASSWR010000000
VERSION     DASSWR010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639613"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFO47_07745"
     CDS             <1..273
                     /locus_tag="VFO47_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET8788625.1"
                     /translation="RLDLRLPDASSLKDKRSVVKGLAAALRKLNCAVAEVDHQDLRQR
                     ATLAVATVAGEGFHARRVLVSAEREAERTPGVELLASDVTMYGPED"
     gene            279..665
                     /gene="rbfA"
                     /locus_tag="VFO47_07750"
     CDS             279..665
                     /gene="rbfA"
                     /locus_tag="VFO47_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET8788626.1"
                     /translation="MAKPSYPRARRLAETIRRLAGEWLEAEQADQPGALMTVTDVRVT
                     EDLHHASVFFTVLGDDEQRAAAEAWLASSAPQARTFVASQLRLRHAPTLEFVPDTVPG
                     QGARIDRLLAELGATPPVPDQEGSGA"
     gene            662..1654
                     /locus_tag="VFO47_07755"
     CDS             662..1654
                     /locus_tag="VFO47_07755"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET8788627.1"
                     /translation="MSIPEQDWQAAVDAIAQAEVAVISCHIHPDGDALGSALALHRAL
                     ANAGREAVVSFSEPFVIARHYRFLAGLDRLVPPGKVPLDADLFLCFDAGSLDRLGTLV
                     DAFRGAARTVVVDHHASNTRFGDVNLIDPVSPASAVLCRELLRRLDLPLDTDIATALY
                     TGLVTDTGRFQYQATTPETHHLAAELLAAGVQQYEVAKAVFETNDIAYLRLVADALGR
                     IVQVPEASLVWTRVDLADLAAHGVEMDETEGLIDLVRTDGASDVAAVLKEQPESGYKV
                     SLRSKGATDVGRLATSFGGGGHKYAAGYTTDSDAQAAVSALVKELVGLGVGLTG"
     gene            1669..2535
                     /gene="truB"
                     /locus_tag="VFO47_07760"
     CDS             1669..2535
                     /gene="truB"
                     /locus_tag="VFO47_07760"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET8788628.1"
                     /translation="MDGVLVCDKAAGMTSHDVVARVRRLAGQRRVGHGGTLDPPATGV
                     LVLALGRATRLLPFLPTEPKRYLATVAFGAETDTLDAAGTVTATADAAAVDEAALRAA
                     MAGFVGPQEQVPPMVSAIKVGGERLYAKARRGEQVERAPRPIVVHALELLGFSAGERP
                     LATVEVVCSGGTYVRSLAADLGRAVGTLAHLAGLRRTAVGRFTEDQAHRLEDLAEEGK
                     LAAAVLDPAAAMDPAAVRALTPDEAAALATGRTLDPTGRGDPVAAVGPDGRLVAVIQD
                     SAGRARPKVVLA"
     gene            2532..>2792
                     /locus_tag="VFO47_07765"
     CDS             2532..>2792
                     /locus_tag="VFO47_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013126379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET8788629.1"
                     /translation="MTVRRFDGVEAVPADLGPTVVTVGMFDGVHRGHRALLDRVTAEA
                     AARGVPAAAVTFDRHPLAVLRPGSEPPLLTTLDRKVELLGEAG"
BASE COUNT          344 a         1084 c         1027 g          337 t
ORIGIN      
        1 cgcctcgacc tccggctgcc ggacgcctcg tcgctcaagg acaagcgctc ggtggtcaag
       61 ggcctggccg ccgcgctgcg caagctcaac tgcgcggtcg ccgaggtcga ccaccaggac
      121 ctgcgccagc gggccaccct ggcggtggcc acggtggccg gcgaaggctt ccacgcccgc
      181 cgggtgctgg tgtcggccga gcgggaggcg gagcgcacgc ccggcgtcga gctgctggcc
      241 agcgacgtga ccatgtacgg acccgaggac tgaacccgat ggcgaagccc agctaccccc
      301 gagcccgccg cctggccgag acgatcaggc ggctggccgg cgaatggctg gaggccgagc
      361 aggccgacca gccaggcgcc ctgatgaccg tcaccgacgt ccgcgtcacc gaggacctgc
      421 accacgccag cgtcttcttc accgtgctcg gcgacgacga gcagcgggcc gccgccgagg
      481 cctggctggc ctcctcggcc ccccaggccc ggaccttcgt ggcctcgcag ctgcggctcc
      541 gtcatgcgcc gacgctagaa ttcgttcccg acaccgtgcc cggccagggt gcgcggatcg
      601 accggttgct ggccgagctg ggcgccaccc cgccggtacc ggaccaggag gggtcaggcg
      661 cgtgagcatc ccggagcagg actggcaggc ggccgtcgac gcgatcgccc aggccgaggt
      721 ggcggtgatc tcctgccaca tccatcccga cggcgacgcc ctcggctccg ctctggccct
      781 gcaccgggcc ctggccaacg ccggccgcga ggcggtggtg agcttctcgg agccgttcgt
      841 gatcgcccgc cactaccgct tcctggccgg gctggaccgg ctggtcccac ccggcaaggt
      901 gccgctggac gccgacctgt tcctctgctt cgacgccggc tcgctcgacc gcctcggcac
      961 cctggtcgac gccttccggg gggccgcccg caccgtggtc gtcgaccacc acgcctccaa
     1021 cacccgcttc ggcgacgtca acctgatcga cccggtgtcg ccggccagcg ccgtgctctg
     1081 ccgcgagctg ctccgccgcc tcgacctccc gctcgacacc gacatcgcca ccgcgctcta
     1141 caccgggctg gtcaccgaca ccgggcgctt ccagtaccag gccaccaccc ccgagaccca
     1201 ccacctggcc gccgagctgc tggccgccgg ggtccagcag tacgaggtcg ccaaggccgt
     1261 gttcgagacc aacgacatcg cctacctgcg gctggtcgcc gacgccctcg ggcgcatcgt
     1321 ccaggtcccg gaggcgtccc tggtctggac ccgggtcgac ctggccgacc tggccgccca
     1381 cggggtcgag atggacgaga ccgaaggcct gatcgacctg gtccgcaccg acggggccag
     1441 cgacgtggcc gccgtgctga aggagcagcc ggagagtggc tacaaggtct ccctgcgctc
     1501 caagggggcc accgacgtcg gccggctggc cacctcgttc gggggcggcg ggcacaagta
     1561 cgccgccggc tacaccaccg actccgacgc ccaggcggcg gtgtccgccc tggtcaagga
     1621 gctggtcggc ctgggcgttg gcctgaccgg gtaggagggc ggccgggcgt ggacggggtg
     1681 ctggtctgcg acaaggcggc cgggatgacc tcccacgacg tggtcgcgcg ggtgcgccgg
     1741 ctggccggcc agcgccgggt cgggcacggc ggcaccctcg acccgccggc caccggggtg
     1801 ctggtcctgg ccctgggccg ggccacccgg ctgctgccgt tcctgcccac cgagcccaag
     1861 cgctacctgg ccacggtcgc cttcggggcc gagaccgaca ccctggacgc ggccgggacc
     1921 gtcaccgcca ccgccgacgc cgccgcggtc gacgaggcgg ccctgcgcgc cgccatggcc
     1981 ggcttcgtcg gcccccagga gcaggtcccg ccgatggtgt cggcgatcaa ggtcggcggg
     2041 gagcggctgt acgccaaggc ccgccggggc gagcaggtcg agcgggcgcc ccgcccgatc
     2101 gtggtccacg cgctggagct gctcggcttc agcgccggag agcggccgct ggccaccgtc
     2161 gaggtggtct gctcgggcgg gacctacgtc cggtccctgg ccgccgacct gggccgggcc
     2221 gtcggcaccc tggcccacct ggccggcctg cggcgcacgg ccgtcggccg cttcaccgag
     2281 gaccaggcgc accgcctgga ggacctggcc gaggagggga agctggcggc ggcggtgctc
     2341 gacccggcgg cggcgatgga cccggcggcg gtccgtgccc tgaccccgga cgaggcggcc
     2401 gccctggcca ccggccggac cctcgacccg accggccgcg gcgacccggt ggccgccgtc
     2461 gggcccgacg gtcgcctggt cgcggtgatc caggacagcg ccggccgggc ccgccccaag
     2521 gtggtgctgg cgtgacggtg cgtcggttcg acggggtcga ggccgtgccc gcggacctgg
     2581 ggccgaccgt ggtcaccgtg gggatgttcg acggggtcca ccggggacac cgggcgctgc
     2641 tggaccgggt gaccgccgag gcggccgcca ggggcgtgcc cgcggccgcg gtcaccttcg
     2701 accgccaccc cctggccgtg ctgcggcccg ggagcgagcc gccgctgctc accaccctgg
     2761 accgaaaggt cgagctgctg ggcgaggccg gc
//
LOCUS       DASSWR010000463         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_215404,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000463 DASSWR010000000
VERSION     DASSWR010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215404"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFO47_07770"
     CDS             <1..807
                     /locus_tag="VFO47_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8788630.1"
                     /translation="EAKSGYGLTVADERRSLEVLARLAERSPVEVVPTFLGAHLIPEE
                     YAADRDGYLDLLEHEMLPACAPLATFVDAFCDRGALTVEESRRVLAAGARHGLKGKLH
                     ANELGSTGGAALAAELGCTSADHLLFCDEQEAKALAAAGTVAVLLPGTSFLLRTGRAA
                     PAQVLRDAGVTMALGTDCNPGTCYSESMQLMVALACVHGGLTPEEAVLAATDGAARAL
                     GRGGRAGRLAPGAACDLVVLAGRSYLDLAYHLGVNLAERVVKAGAVVAGP"
     gene            804..1772
                     /gene="ftcD"
                     /locus_tag="VFO47_07775"
     CDS             804..1772
                     /gene="ftcD"
                     /locus_tag="VFO47_07775"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET8788631.1"
                     /translation="MTELTRLVECVPNVSEGRRPEVVDEIVAAFAGAGPEVLVLDTSS
                     DPDHHRTVITLMGPGPALVEAAVAGARTCARLIDLNHHHGVHPRMGALDVLPFVPFGA
                     ETRLRGGDDPDLDCADLAERAGRRIAAEAGVPVYLYGAAARHPGWTALPAVRGRGFEA
                     LRSALAAGAPDPPAPDFGGPGLHPTAGAVAAGAREVLVAYNVELAGADLDLARKIASA
                     VRERDGGLPAVRAMGVALPGRGLVQVSMNLLDYRSTPPAAAYAAVAALAEQAGARVEA
                     SEIVGLVPAGALDGVDPASLRLRGLATNLFLESRLLQALSISKERR"
     gene            1769..2398
                     /locus_tag="VFO47_07780"
     CDS             1769..2398
                     /locus_tag="VFO47_07780"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HET8788632.1"
                     /translation="MTPQNRLGEHGWLDTYAAPTVTPGGGSASAVAAALGCALLARTA
                     RIGARKADPDAAGRLGQLAGEADRLRAALVEQERTDADVYERMVATVRASRATEGDPG
                     AARAAEAARADATEVPLATAELAAEGLALAGEVAGMGLARTASDQLVAAHLLRAGLEG
                     GLATIAFNLRRMAAGPVRDDLAARAARLDDARRAAADLTQRLVAMVEQP"
     gene            2461..>2801
                     /locus_tag="VFO47_07785"
     CDS             2461..>2801
                     /locus_tag="VFO47_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788633.1"
                     /translation="MARDVQQPETTGMTRLPDTTGPGDPHDFPPEDPAVPRARPGAYW
                     TGLVVLVAGLAVTVAFLATRVTSIVQTVLLAVPLALIGIGLERVGRGTGRGSLRSVGV
                     VLLAVAVVGPLV"
BASE COUNT          300 a         1138 c         1037 g          326 t
ORIGIN      
        1 gaggccaagt ccggctacgg cctcaccgtc gccgacgagc gccgcagcct ggaggtgctg
       61 gcccggctgg ccgagcggtc cccggtggag gtggtcccga ccttcctcgg cgcccacctg
      121 atccccgagg agtacgccgc cgaccgcgac ggctacctgg acctgctgga gcacgagatg
      181 ctgcccgcct gcgcgcccct ggccaccttc gttgacgcct tctgcgaccg gggggcgctg
      241 acggtggagg agtcccggcg ggtcctggcc gccggcgccc gccacggcct caagggcaag
      301 ctccacgcca acgagctcgg ctccaccggc ggggccgccc tggccgccga gctcggctgc
      361 acctccgccg accacctgct gttctgcgac gagcaggagg ccaaggccct ggccgccgcc
      421 ggcacggtgg cggtgctgct gcccgggacc agcttcctgc tccgcaccgg ccgggcggcc
      481 ccggcccagg tgctgcgcga cgccggggtc accatggccc tgggcaccga ctgcaacccc
      541 ggcacctgct acagcgagtc gatgcagctg atggtggcgc tggcctgcgt gcacggcggc
      601 ctcacccccg aggaggcggt gctggccgcc accgacggcg ccgcccgggc gctggggcgc
      661 ggagggcggg ccgggcggct cgcccccggg gccgcctgcg acctggtcgt gctggccggg
      721 cggagctacc tcgacctggc ctaccacctc ggggtcaacc tggccgagcg ggtcgtcaag
      781 gccggagcgg tggtggccgg gccgtgaccg agctcacccg cctggtcgag tgcgtgccca
      841 acgtctcgga gggccgccgg cccgaggtgg tcgacgagat cgtggccgcc ttcgccgggg
      901 ccggccccga ggtgctggtg ctcgacacct ccagcgaccc cgaccaccac cgcacggtca
      961 tcaccctgat ggggcccggg ccggccctgg tcgaggcggc cgtggccggg gccaggacct
     1021 gcgcccgcct gatcgacctc aaccaccacc acggcgtcca cccgcgcatg ggggcgctgg
     1081 acgtgctgcc gttcgtgccc ttcggggccg agacccggct ccgcggcggc gacgaccccg
     1141 acctggactg cgccgacctg gccgagcggg ccggccggcg catcgccgcc gaggccggcg
     1201 tccccgtcta cctgtacggg gccgcggccc gccacccggg atggacggcc ctgccggccg
     1261 tccgcggccg cgggttcgag gccctccggt ccgccctggc cgccggcgcc ccggacccgc
     1321 ccgcacccga cttcggcggg ccgggcctgc acccgaccgc cggggccgtc gccgccgggg
     1381 ccagggaggt cctggtcgcc tacaacgtcg agctggccgg cgccgacctg gacctggccc
     1441 ggaagatcgc gtccgcggtc cgggaacgcg acggcggcct gcccgcggtc cgggccatgg
     1501 gggtcgccct cccgggtcgg ggactggtcc aggtgtcgat gaacctgctc gactaccggt
     1561 cgaccccgcc ggcggccgcc tacgccgccg tggccgccct ggccgagcag gccggggcca
     1621 gggtcgaggc ctcggagatc gtcggcctcg tcccggccgg cgccctcgac ggggtcgacc
     1681 cggccagcct gcgcctgcgc ggtctcgcga cgaatctgtt cctcgaatcg cggctgctcc
     1741 aggccctctc catctccaag gagcgtcgat gactccccag aaccggcttg gcgagcatgg
     1801 ctggctggac acctacgccg ccccgaccgt caccccgggc gggggcagcg cctcggcggt
     1861 ggccgccgcc ctcggctgcg ccctgctggc caggacggcc aggatcggcg cccgcaaggc
     1921 cgacccggac gcggccgggc ggctcggcca gctcgccggc gaggccgacc ggctccgggc
     1981 ggccctggtc gagcaggagc ggaccgacgc cgacgtgtac gagcgcatgg tggccaccgt
     2041 ccgcgcctcc cgagccaccg aaggtgaccc gggggcggcc agggcggccg aggcggcccg
     2101 ggccgacgcc accgaggtcc cgctggccac ggccgagctg gccgccgagg gcctcgccct
     2161 ggccggcgag gtggccggga tgggcctggc ccggacggcc agcgaccagc tggtcgccgc
     2221 ccacctgctc cgggccggcc tggagggcgg cctggccacc atcgccttca acctgcgccg
     2281 catggccgcc ggcccggtcc gcgacgacct ggccgcccgc gccgcccgcc tcgacgacgc
     2341 ccgccgggcc gccgccgacc tcacccagcg gctggtcgcc atggtcgagc agccatgatt
     2401 tccccgtcgc ccggcgatcg gggtttactc ccccgggaag cccagcggga ggagtgaggg
     2461 gtggccaggg acgtgcagca gccggagacc accggcatga cccgcctgcc cgacaccacc
     2521 ggacccggcg acccgcacga cttccccccc gaggacccgg ccgtccccag ggcgcggccc
     2581 ggcgcctact ggaccggcct ggtcgtcctg gtcgccggcc tggccgtcac cgtcgccttc
     2641 ctcgccaccc gggtcacctc gatcgtccag accgtcctgc tggccgtccc cctggccctc
     2701 atcgggatcg gcctggagcg ggtgggccgg ggcaccggcc ggggctcgct gcgctcggtc
     2761 ggcgtcgtcc tgctggccgt ggccgtggtc ggcccgctgg t
//
LOCUS       DASSWR010000464         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_328765,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000464 DASSWR010000000
VERSION     DASSWR010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328765"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1252)
                     /locus_tag="VFO47_07790"
     CDS             complement(107..1252)
                     /locus_tag="VFO47_07790"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788634.1"
                     /translation="MVVVLVLVLVFFAGGGWFYSGEIRAGALDSKAPDPLPLSTEVLA
                     VGDGSITLAREPGSPRELTLPGTWGLRWADGYGRLGAIQAQGPDRVERAFTRLEGTPP
                     RAGDDTAVEGYAFPADPALAAGRPAREVSYPSPLGPAAAWQVEGRRDTWVILVHGYNA
                     ARTETLRTLATVSHQGYPALAITYRNDPGAPRSPDGLRHWGATEWRDLEAATRYAFDQ
                     GAGGVVLAGFSMGGAVVTSFLLESPLAPRVRGVVLASPALDLGEVIDHGAADRELPVL
                     GTPVPPALTVAAKGVAGARYDLDWGQLDYVDRAGGLAAPMLVFHQTGDPTVPVAISEA
                     LAAARPDLVILERFAGDGHVQSWNTDRARYEGTLRAFLDRVAPASAA"
     gene            complement(1320..>2023)
                     /locus_tag="VFO47_07795"
     CDS             complement(1320..>2023)
                     /locus_tag="VFO47_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET8788635.1"
                     /translation="AARHCRNAMTRGDRFRRGVVALSLVLAGAAGCTGGQPRGERVTH
                     AAPPASRSAVAGATSTQPPAGPTRSTEVIDFPVGSWRRLPPSPPVRVEIPAIGVSSSL
                     VRLGLNRDRTMEVPGDDQVAGWFTGGPQPGQLGPAVIAGHVDSRTGPAVFYRLRDLRP
                     GDQIRVVRADGSHVRFVVEGAGSHPKDGFPTGAVFGPVPGAALRLITCAGDFDRARGS
                     YRDNLVVFARLAGTG"
BASE COUNT          259 a          774 c          767 g          223 t
ORIGIN      
        1 aggtcggccg cctgcgggag ctgggctgcg actacggcca gggctaccac ttcggccggc
       61 ccttaccacc cgacgacctg accgcccgcc tcagcagggc cgccgctcag gctgccgatg
      121 ccggcgccac ccggtccagg aaggcccgca gcgtcccctc gtagcgggcc cggtcggtgt
      181 tccaggactg gacgtggccg tcgccggcga accgctccag gatgaccagg tcggggcggg
      241 ccgcggccag ggcctcgctg atcgccaccg ggacggtcgg gtcgccggtc tggtggaaga
      301 ccagcatggg tgcggccagc ccgccggccc ggtcgacgta gtcaagctgg ccccagtcca
      361 ggtcgtagcg ggccccggcg acacccttgg cggccaccgt gagtgccggc ggcaccgggg
      421 tcccgagcac cggcagctcc cggtcggcgg caccgtggtc gatcacctcg cccaggtcga
      481 gggccggcga ggccagcacc accccccgga cccggggggc cagcggcgac tccagcagga
      541 agctggtcac gaccgcgccg cccatgctga agccggccag gaccacgccg cccgcgccct
      601 ggtcgaacgc gtaccgggtc gccgcctcca ggtcacgcca ctcggtcgcc ccccagtggc
      661 gcaggccgtc gggggagcgg ggcgcgcccg ggtcgttgcg gtaggtgatg gccagggccg
      721 ggtagccctg atggctgacc gtggccagcg tccgcagggt ctcggtgcgg gcggcgttgt
      781 agccgtggac caggatcacc caggtgtcgc gccggccctc gacctgccag gccgccgccg
      841 ggccgagcgg cgaggggtag ctgacctcgc gggccggccg gcccgcggcc agggccgggt
      901 cggccgggaa ggcgtagccc tcgacggctg tgtcgtcgcc ggcccggggc ggggtcccct
      961 ccaggcgggt gaacgcccgc tcgacccggt ccggtccctg ggcctggatg gccccgagcc
     1021 gcccgtagcc gtccgcccag cgcaggcccc aggtgccggg aagggtcagc tcccggggcg
     1081 agcccggttc gcgggccagg gtgatcgagc cgtcgccgac ggccaggacc tcggtgctga
     1141 gcgggagtgg gtccggcgcc ttgctgtcca gggcgccggc ccggatctcg cccgagtaga
     1201 accagccgcc gccggcgaag aagaccaaca ccagcacgag gacgacgacc acagcgccgc
     1261 gcaacagccg ccatggccgc gacccgcgtc ggccagctcc ggttcccacg cccctatcat
     1321 cacccggttc cggccagccg ggcgaacacc accaggttgt cgcggtagct gccccgggcc
     1381 cggtcgaagt cgccggcgca ggtgatgagc cgcagggccg ccccggggac cggcccgaac
     1441 accgcgccgg tggggaagcc gtccttgggg tggctgcccg ccccctcgac cacgaaccgc
     1501 acgtggctgc cgtcggccct gacgacccgg atctggtcgc cggggcgcag gtcgcgcagc
     1561 cggtagaaca cggccggccc ggtccgggag tcgacgtggc cggcgatcac ggccgggccc
     1621 agctggcccg gctgaggacc accggtgaac cagccggcca cctggtcgtc gccgggtacc
     1681 tccatggtcc ggtcgcggtt gagcccgagc cggaccaggg acgaggacac gccgatggcc
     1741 gggatctcga cccgcaccgg gggcgagggc ggcaggcggc gccacgaccc aaccgggaag
     1801 tcgatgacct cggtggagcg ggtcggcccg gccggcggct gggtgctggt ggctccggcg
     1861 accgcggaac ggctggccgg gggagcggcg tgcgtcaccc gctcccccct cggctggccg
     1921 ccggtgcacc cggcggcgcc ggcgagcacc agactcagcg cgaccacccc tcggcggaac
     1981 ctgtctcccc tggtcatggc attccgacag tgcctagccg ctc
//
LOCUS       DASSWR010000465         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_977973,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000465 DASSWR010000000
VERSION     DASSWR010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977973"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFO47_07800"
     CDS             <1..912
                     /locus_tag="VFO47_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8788636.1"
                     /translation="YWVSGWVARLARERWDSPVAHSFHTLGRVKNLSLADGDVPEPPA
                     RLAGEERVVAAADCLLAPTPAEARQLVELYGASPAKVQIVPHGVDRGRFHPGDKAAAR
                     AALGIAHRNVLAFVGRLQPLKAPDVAVLALAELCRRRPDLDVELLVVGGPSGNGDHEP
                     ARLARLAAANGVADRVRFLSPQPHDRLATIYRAADLVLMPSWSESFGLVALEAQACGT
                     PVVAAGVGGLLHAVGDGTTGVLLANHQPDCWADIVAQLLSSPRRLAAMGAAAARFAGA
                     HGWDQAAARLIEIYGDLVQEPVLEQMG"
     gene            912..1604
                     /locus_tag="VFO47_07805"
     CDS             912..1604
                     /locus_tag="VFO47_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET8788637.1"
                     /translation="MLAIRRALAIGAHPDDVELQAGGTLAAWAGRGVHVELACLTAGE
                     KGSADPAADPERLAGVRRAEAEAAARSLGTAVPVHFLGAVDGELEVTMALRLAVARLV
                     RTVRPDVVLGHDPWRRDLLHPDHRAAGLLTVDGVVAARDPLYAPTLAAEGLAPHRPHT
                     VLLFGTDAPDELVDVTVTIDAKLASLRAHASQIGDHADLERRVRTWNAAIGAGAGLLY
                     AEAFHTLDTRGR"
     gene            complement(1641..2438)
                     /locus_tag="VFO47_07810"
     CDS             complement(1641..2438)
                     /locus_tag="VFO47_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788638.1"
                     /translation="MTTDYGGSGDPARSLPLLWRDQKRPTRGPRPGLTVDRIVAAAIE
                     LADAEGLAALSMRRVAERLGVGTMSLYTYVPGKAELLDVMLDTVLGEVARPDGAGGWR
                     AGLELRAREDWALFHRHPWMLQISPSRALLGPNETELFEAALRVVDGIGLGGSEMVLV
                     VSLVADYTRGAADMAVGAAEAERRTGISDEQWWAAREPLFDKYFDPSRYPTVVRVAAA
                     GAFEQPAGDAAYTVAQAQESFEFGLARVLDGIEAFVRARSRPSADDR"
     gene            2511..>2705
                     /locus_tag="VFO47_07815"
     CDS             2511..>2705
                     /locus_tag="VFO47_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788639.1"
                     /translation="MATLPVLAEGLRKRYGRANALDGFDLAVAEGSVCGLLGPNGAGK
                     TTAVRILTTLLRPDAGRARVA"
BASE COUNT          318 a         1083 c          975 g          329 t
ORIGIN      
        1 tactgggtgt cgggctgggt ggcccggctg gcccgggagc gctgggacag cccggtggcc
       61 cactccttcc acaccctcgg ccgggtcaag aacctgagcc tggccgacgg cgacgtgccc
      121 gagcccccgg cccggctggc cggcgaggag cgggtcgtgg ccgccgccga ctgcctgctc
      181 gccccgaccc cggccgaggc ccgccagctg gtcgagctgt acggcgcctc cccggccaag
      241 gtccagatcg tgccccacgg cgtcgaccgg ggccgcttcc acccgggcga caaggccgcg
      301 gccagagccg ccctcgggat cgcccaccgc aacgtgctgg ccttcgtcgg ccgcctccag
      361 ccgctcaagg cgcccgacgt ggccgtgctg gctctggccg agctgtgccg ccggcggccc
      421 gacctggacg tcgagctgct ggtggtcggg gggccgtcgg gcaacggcga ccacgaaccg
      481 gcccgcctgg cccggctggc cgccgccaac ggggtcgccg accgggtccg gttcctctcg
      541 ccgcagcccc acgaccgcct ggccaccatc taccgggcgg ccgacctggt cctgatgccg
      601 tcctggtcgg agtcgttcgg cctggtcgcc ctcgaggccc aggcctgcgg caccccggtg
      661 gtcgcggccg gggtgggcgg gctgctgcac gccgtgggcg acggcaccac cggggtcctg
      721 ctcgccaacc accagccgga ctgctgggcc gacatcgtcg cccagctcct gtccagcccc
      781 cgccgcctgg ccgccatggg cgcggccgcc gcccgcttcg ccggcgccca cggctgggac
      841 caggcggccg cccgcctgat cgagatctac ggcgacctgg tccaggagcc ggtgctggag
      901 cagatgggct agtgctggcc atccgccggg ccctggccat cggcgcccac ccggacgacg
      961 tggagctgca ggcaggcggg accctggccg cctgggcggg gcggggcgtc cacgtcgagc
     1021 tggcctgcct caccgccggg gagaagggca gcgccgaccc ggcggccgac ccggaacggc
     1081 tggccggggt gcggcgggcc gaggcggagg cggccgcgcg gtccctgggg acggccgtcc
     1141 cggtccactt cctgggggcg gtggacggcg agctcgaggt caccatggcc ctgcgcctgg
     1201 ccgtggcccg gctggtccgg acggtgcggc ccgacgtcgt cctcggccac gacccgtggc
     1261 gccgggacct gctccacccc gaccaccggg ccgccgggct cctcaccgtc gacggcgtgg
     1321 tcgcggcccg cgaccccctc tacgccccaa ccctcgccgc cgagggtctg gccccgcacc
     1381 ggccgcacac ggtgctgctg ttcggcaccg acgcccccga cgagctggtc gacgtgaccg
     1441 tcaccatcga cgccaagctg gcctcgctgc gcgcccacgc cagccagatc ggcgaccacg
     1501 ccgacctgga acggcgggtc cgcacctgga acgcggccat cggcgccggc gccggcctcc
     1561 tttacgccga ggccttccac accctcgaca cccggggccg ctgaccgacc ggcccgaccg
     1621 gcgagttcac cggtcggagc ctaccgatcg tcggccgaag gccgactcct cgctctgacg
     1681 aacgcctcga tgccgtcgag gacgcgggcc aggccgaact cgaagctctc ctgggcctgg
     1741 gcgacggtgt aggcggcgtc gccggccggc tgctcgaagg cgcccgcggc ggccaccctg
     1801 accaccgtcg ggtagcggct cgggtcgaag tacttgtcga acagaggctc gcgggccgcc
     1861 caccactgct cgtcggagat gccggtgcgc cgctcggcct cggcggcccc caccgccatg
     1921 tcggccgcgc ccctggtgta gtcggcgacc aggctgacca ccagaaccat ctcggagccg
     1981 ccgaggccga tcccgtcgac cacccgcagg gcggcctcga acagctcggt ctcgttgggc
     2041 ccgagcagcg cccgggacgg ggagatctgc agcatccagg ggtggcggtg gaacagggcc
     2101 cagtcctcgc gggcccgcag ctccagcccg gcccgccagc ccccggcccc gtccggccgg
     2161 gccacctcgc ccagcaccgt gtccagcatg acgtcgagca gctcggcctt gccgggcacg
     2221 taggtgtaga gcgacatggt cccgaccccg agccgctcgg cgacccggcg catggacagc
     2281 gcggccaggc cctcggcgtc ggccagctcg atggcggcgg ccacgatccg gtccacggtc
     2341 aggccgggcc gcggcccccg ggtcgggcgc ttctggtccc gccagagcag cggcaggctc
     2401 cgcgccgggt cgccgctgcc gccgtagtcg gtcgtcaccc ttgcaactcc gtatggtgta
     2461 cggtactctt ccccgtgact ccgtacagtg tatcgagtaa ggcggtgcag atggcaacgc
     2521 tacccgtcct ggccgagggg ctgcgcaagc gctacggccg ggccaacgcc ctcgacggct
     2581 tcgacctggc cgtcgccgag ggctcggtct gcgggctgct cggccccaac ggcgccggca
     2641 agaccaccgc ggtgcggatc ctgaccaccc tgctgcggcc cgacgccggc cgggcccggg
     2701 tggcc
//
LOCUS       DASSWR010000466         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1233311, whole genome shotgun sequence.
ACCESSION   DASSWR010000466 DASSWR010000000
VERSION     DASSWR010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233311"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..661)
                     /locus_tag="VFO47_07820"
     CDS             complement(212..661)
                     /locus_tag="VFO47_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788640.1"
                     /translation="MSQSTDDLTRSELRAIRGHYRRRVWLAFLVPALIAVALGWLLFQ
                     DQDANSGEPSQATPRTTVSGPITNEVVSKNCVAALQEASTMLDRAAQALKDWRLHAKA
                     LEDYREGQISLAEARQRWDQTTKVGLAGADRFDKQAEVYKKAATTCK"
     gene            931..1335
                     /locus_tag="VFO47_07825"
     CDS             931..1335
                     /locus_tag="VFO47_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788641.1"
                     /translation="MSEAVTMSVEPPTPSAEQLADWQALVERIRQGDRSQALAWDEVA
                     ATRPKTAYQVLVTQQVHRRLRGAPPLLAGSVAGVVAVLRVDPTEASMIFRRRPLGDGG
                     WTVTSGTAQGSLTYWVLQAERMVVLLDLTWAE"
     gene            1786..2496
                     /locus_tag="VFO47_07830"
     CDS             1786..2496
                     /locus_tag="VFO47_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET8788642.1"
                     /translation="MLPRRERRVVRVVDGGGAMTAYQPDAEAGRPNDEGPPAFQVVRR
                     GYDREEVDAYFSQLAARLNEAVDQYAKAEQARAELQDEVTSLQQRAPSFEQVGGEAAA
                     LLAEAGRSAEQLVENGRRRADTIIEKAQQQAEQLRADVTAEAQRVLEQAREVAEQIRA
                     EVEQERAVVVRETDQVREFRDGLLDDLGRVHGEISGLLERTRKQRDEAVDAGGADPKA
                     AAAGNGKAKKPATVTDRK"
BASE COUNT          445 a          822 c          952 g          456 t
ORIGIN      
        1 cagctcctcg gcggtggcgc gggcggccac catcccagcg gccttggcga tggcctcata
       61 gcgggtctgg ctggcgtggt cctggtcgat caagtcggcc atgccacgaa acaaccgagc
      121 cagcggacga atctggttgc ccatcgcaag cccctgagcc ttggctgatc accgacgcgg
      181 gtggctattt cattcaccac gggttggtcg tctatttgca ggtggtggcg gccttcttgt
      241 agacctcggc ttgcttgtcg aagcggtcgg caccggccag ccccaccttg gtggtctgat
      301 cccaccgttg tcgggcctcg gccaggctga tctggccctc ccggtagtct tccagggcct
      361 tggcatgcaa gcgccagtcc ttgagggcct gggcggcccg gtccagcatg gtgctggcct
      421 cctggagggc ggccacacag ttcttggaca cgacctcgtt ggtgatcggg ccgctgacgg
      481 tggtccgggg cgtcgcttgg gacggctccc ccgagttggc gtcttggtcc tggaacaaca
      541 gccagccaag cgccacggca atgagtgccg ggacgaggaa ggcaagccag acgcgccggc
      601 ggtagtggcc gcggatggcc cgtagctccg agcgggtcag gtcatcggtc gactgggaca
      661 tcacggcacc tccctaccgg ggcgcagtct taccgtagct ggaaggtgac aaacggtccc
      721 cgccccaacc tgacaatctt ctgccctcaa tgagggttgc gcttatagct cttgctgagg
      781 cagatgcggc gattaataga tactaggtac tctgcgtatg aacctgatat atgcagaatg
      841 ttccttctcg accgacccta cgccgccgta gatacacaat tgtgcccagc tcggtgggag
      901 ccagggttcg gtggccgata ggctggttgc atgagtgagg cggtgaccat gtcggtcgag
      961 ccaccaacgc cctcggccga gcagcttgcc gactggcagg ccctggtcga gcggatccgc
     1021 cagggcgatc gctcccaggc gctggcctgg gatgaggttg cagctactcg gcccaaaacg
     1081 gcctaccagg tccttgtgac gcagcaggtt caccggcggc tgcgcggcgc cccgcccctg
     1141 ctagccggct cggtggctgg cgtcgtcgcg gtccttcggg tggatccgac cgaagccagc
     1201 atgatctttc gccgacgccc cctgggtgat ggcggctgga cagtgacctc cggcaccgcc
     1261 caaggatcac tgacctactg ggtcctgcaa gccgagcgga tggtggtgct gctcgacctg
     1321 acctgggcgg aatgactagc aacaggtggc atgccagctg cgggtggtac gggatcgacc
     1381 ggggaggtca tcgggacgcc aagcgccgtc tgatcgtcac catccatgct ggttgctgtg
     1441 ggtgtgggtg tgtggacaaa cggttgatca gctcctcctc tggcctggac cgtctggaga
     1501 gcttcgaccg aatccaacgg taaggtgctg gtgcgaattg ggcgcatcct ggcagttagc
     1561 ggccgaggtg tagaacagaa cagccaaaga atgcggccct catggcgtct gagctacaca
     1621 gagggtaaat ggcagcagct tatttcttcc ctgacaggtc ccacgcaggt cgcgccatca
     1681 tgatggccga aattcctagg agtcggctgg agctgacgca cccggatgcc ggcgcgcggt
     1741 tgactgtgtt caccgatttg cgctgtaggt gctggagatg cgatcttgct gcctcggcgc
     1801 gagcgacggg ttgtgcgcgt cgtggatgga gggggtgcca tgaccgcgta ccaaccggac
     1861 gcggaggctg gacggcccaa cgacgaggga ccgccagcct ttcaggtggt gcggcgtggg
     1921 tacgaccgcg aggaggtcga cgcctacttc tcccagctcg ccgcgcgtct gaacgaggcg
     1981 gtcgaccagt acgccaaggc cgagcaggca cgggccgagc tccaggacga ggtcaccagc
     2041 ctccagcagc gagcgccgtc cttcgagcag gtcggcgggg aggccgcggc gctgttggcg
     2101 gaggccggcc gctcggccga gcagttggtg gagaatggcc ggcgccgggc cgacacgatc
     2161 atcgagaagg cccagcagca ggccgagcag ctgcgggccg atgtcaccgc cgaggcgcag
     2221 cgggtgctgg agcaggcccg ggaggtggcc gagcagatcc gcgccgaggt ggagcaggag
     2281 cgggcggtgg tggtacgcga gaccgatcag gtgcgcgagt tccgcgatgg gctgttggat
     2341 gacctgggtc gggtgcatgg cgagatcagc gggctgctgg agcggacccg caagcaacgc
     2401 gacgaggcgg tggacgccgg cggcgccgac ccgaaggctg cggccgcagg gaacggcaag
     2461 gccaagaagc cggcgacggt cactgaccgc aagtagctga ccgctcggcg ggccgctcag
     2521 gatggggtca atccgcttac gcttcgttac cggagccccc aacgtgctgc gcccccggct
     2581 gagccgaggg cgctaaggcg tgatagatca gaacggcggc gcatcgttga agtcgccacc
     2641 gcgttcggcc tgccgctccc gtccctgagc ccggt
//
LOCUS       DASSWR010000467         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_780369,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000467 DASSWR010000000
VERSION     DASSWR010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780369"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFO47_07835"
     CDS             complement(<1..843)
                     /locus_tag="VFO47_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017534163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HET8788643.1"
                     /translation="MAGRIRDESIAELRQATDVVALVGEYVTLRPGGGTRMKGLCPFH
                     QEKTPSFTVDSSRGLWHCLAGETRVLTWEGVRPIRELAGGTHRILARNGDWVEAPFHS
                     FGVQPLMRLVVSRNGQVKELFATAEHRWFVGRASRLSDREVVTRELRPGQRLVPKFPK
                     TLVKRVTPSAFGIAHGITYGDGSFLDSGSRAELDPIKDAPLLKWFPHSRVTQRGPHLR
                     VYDLPRFFKQLPPLDESVSYLYGWLAGYVAADGHVAKDGTVMLHCASRETLEYVRAVC
                     TRLGI"
     gene            complement(1001..2059)
                     /locus_tag="VFO47_07840"
     CDS             complement(1001..2059)
                     /locus_tag="VFO47_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET8788644.1"
                     /translation="MTATHDWVQRGTVRAELEAAEARTLAPWAAKAASSAGRAQPEPE
                     DPVRTCWMRDLDRIIFSRAMMRAHGKTQSFIPAFSGEGPAAGRQGGPYIGDHYVTRAT
                     HMQQTARIAATIAQALALNVPLATAIATGHDLGHACFGHGGEAALQQVAPGGFDHARQ
                     GARLLTLLEPRNLTAEVLEGIARHSWKHEPPSTLEGLCARLADRIAYLTADLTDALRA
                     GVLTGVDQVPAGVRRVLGEQPADMIGVLVEDVIRHSRGAPRVVQGDACAEAMSVMRSF
                     MFEHVYLRPEAERQTERATRLLTDLYGHYLEHPDTMPMGASLATDPVAQQAVDAIAGM
                     TDRYALAAWKAAFAPEAA"
     gene            complement(2129..>2533)
                     /locus_tag="VFO47_07845"
     CDS             complement(2129..>2533)
                     /locus_tag="VFO47_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8788645.1"
                     /translation="TFDRIGVEDLAVKHLSRRGAGRHKAGLNRSIADAGWAGFLRVLV
                     WQAAKAGKQIVVLPACNTTQECSACGAKAKPRLELSDRIFRCWSCGLVLDRDRNSAWN
                     LHPDHPRNNRLGCTGAGDDGDKTLVPAGTRAT"
BASE COUNT          373 a          857 c          931 g          372 t
ORIGIN      
        1 gatgcccagg cgcgtgcaga ctgctcgaac gtattcgaga gtctccctgc tcgcgcagtg
       61 gagcatgacc gtgccgtcct tggcgacatg gccgtcggcg gcgacgtagc ccgccaacca
      121 gccgtagagg taggagaccg actcatcgag cggtggcagc tgcttgaaga accgtggcag
      181 gtcatatacc cgcagatgcg gaccacgttg agtcacccga ctgtgcggga accacttgag
      241 gagcggcgcg tccttgatcg ggtcaagctc cgccctgctg cctgagtcca gaaaagagcc
      301 gtcgccgtac gtgatgccgt gtgcgatccc gaacgcggag ggcgtcactc tcttgaccag
      361 agtcttcggg aacttcggca ccagccgctg tccaggccgg agctcgcgcg tgacgacctc
      421 ccggtcggaa agacggctgg cgcggccgac gaaccaccgg tgctccgctg tcgcgaacag
      481 ctctttgacc tgcccgtttc ggctgaccac gagccgcatc agcggctgga cgccgaacga
      541 gtggaacggg gcctcgaccc aatcgccgtt acgggccagg atgcggtggg tgccgccggc
      601 cagctcgcgg atcggccgga cgccctccca ggtcagcacc cgggtctccc cggccaggca
      661 gtgccagagc ccgcggctgg agtcgaccgt gaacgagggg gtcttctcct ggtggaaggg
      721 gcagaggccc ttcatgcggg tgccgccgcc ggggcggagg gtcacgtact cgccgaccag
      781 ggcgacgacg tcggtcgcct ggcggagctc ggcgatcgac tcgtcacgga tgcggccggc
      841 cacggcggac ttcacctcga cgcatgacgg aaggacctac gctgcccaag gggaaggacc
      901 cgtcgggtca cccgggcatt gtacgcacga cccccgacgg tggctcagcg gtcgcggcgg
      961 cgggcggagg acacccccgc cgccccgggc caagaacccc ctaggccgcc tcgggggcga
     1021 aggccgcctt ccaggcggcc agggcgtagc ggtcggtcat gccggcgatg gcgtcgacgg
     1081 cctgctgggc gaccgggtcg gtggccaggc tggcccccat gggcatggtg tcggggtgct
     1141 ccaggtagtg gccgtagagg tcggtgagca ggcgggtcgc ccgctcggtc tggcgctcgg
     1201 cctcggggcg caggtagacg tgctcgaaca tgaacgagcg catgaccgac atggcctcgg
     1261 cgcaggcgtc gccctggacg acccgggggg cgccgcggga gtggcggatg acgtcctcga
     1321 ccaggacccc gatcatgtcg gcgggctgct cgccaaggac ccggcggacc ccggccggga
     1381 cctggtcgac ccccgtcagg accccggccc ggagggcgtc ggtgaggtcg gcggtgaggt
     1441 aggcgatgcg gtcggccagg cgggcgcaga gcccctccag ggtcgagggc ggctcgtgct
     1501 tccaggagtg gcgggcgatc ccctccagca cctcggcggt gaggttgcgg ggctcgagca
     1561 gggtgagcag gcgcgccccc tggcgggcat ggtcgaagcc gccgggggcg acctgctgca
     1621 gcgccgcctc gccgccgtgg ccgaagcagg catggcccag gtcgtggccg gtggcgatgg
     1681 ccgtggccag gggcacgttg agggccaggg cctgggcgat ggtggcggcg atccgggccg
     1741 tctgctgcat gtgggtggcc cgggtgacgt agtggtcgcc gatgtagggg ccgccctggc
     1801 ggccggcggc cgggccctcg ccggagaagg cggggatgaa cgactgggtc ttgccgtggg
     1861 cccgcatcat ggcccgggag aagatgatcc ggtccaggtc ccgcatccag caggtccgca
     1921 ccgggtcctc cggctcgggc tgggcccgcc cggccgagga agccgccttg gcggcccagg
     1981 gggccagggt gcgcgcctcg gccgcctcca gctcggcccg caccgtcccc cgctgcaccc
     2041 agtcgtgggt tgccgtcacc ttcgcctccg atgtgtgacc catcactttg actgctccaa
     2101 tcggctggcg ccgcttggat cttggggctc aggttgccct ggttcccgcg ggaaccaggg
     2161 tcttatcgcc gtcatcgccc gcaccggtac agcccaaccg gttgttgcgc ggatggtcag
     2221 gatgcaaatt ccatgccgag ttccggtccc ggtcgaggac tagaccgcag ctccaacacc
     2281 ggaaaattct gtcggagagc tccaagcggg gcttggcttt cgccccgcag gccgaacact
     2341 cctgggtggt attgcaggca ggcagtacga caatctgctt gccggccttt gcggcctgcc
     2401 aaaccaacac tcgcaggaac cccgcccagc cggcatcggc aatcgagcgg ttcaagccag
     2461 ccttgtgacg ccctgcgcca cgacgggaca agtgcttcac cgccaggtcc tcgacgccga
     2521 tccggtcgaa tgt
//
LOCUS       DASSWR010000468         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_865362,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000468 DASSWR010000000
VERSION     DASSWR010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865362"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFO47_07850"
     CDS             complement(<1..260)
                     /locus_tag="VFO47_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788646.1"
                     /translation="MRITVVGPADLPELLPLMRAYCDFYQVAPADQALLELSQALLAD
                     PQREGLQLLARDDTGRAVGFATLFWTWQTLAAARVGVMNDLYV"
     gene            complement(555..>2093)
                     /locus_tag="VFO47_07855"
     CDS             complement(555..>2093)
                     /locus_tag="VFO47_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8788647.1"
                     /translation="HFGDTLREGYFLADPRVVELLAREAPAAVLELADWGAPFARTPD
                     GRLDQRFFGAHRWRRTCYAGDWTGRAILRTLAAKVAELGLTVIDDQYVSQLLVADGVC
                     FGALAFDLETGARTVFLADAVVLAAGGHTRIWRRSSSRRDENTGDGMYLALRAGCRLA
                     DMELVQFHPTGMVAPEEAAGTLVTEAVRGEGGQLKNALGERFMGRYDPERMELSARDR
                     VALANYTEIAQGRGGPNGGVFLDISHLGKDVILEKLPRMYRQFLELQMLDISQQPMEV
                     APTAHYSMGGVVVDPETHATDVAGLYAAGEIAAGLHGANRLGGNSLAETVVFGRRAGE
                     AAARYSASRDVALRARGVVRVADQELSGFIRYGRQFARPLQRALRDTMWETCGVVRDQ
                     AGLQRGLERLAELRGLAADVDVRPTSEGYGDLAHALDLRASLAAAEATLLGALARTES
                     RGAHQRRDHPELDPELRVNFQARLDGTGRLAVDARPVPAVPAELAGWTRPDPDLSVAG
                     RLLE"
BASE COUNT          307 a          788 c          751 g          247 t
ORIGIN      
        1 acgtacaggt cgttcatcac cccgacgcgg gcggcggcca gcgtctgcca ggtccagaag
       61 agggtggcga agccgaccgc ccggccggtg tcgtcgcggg ccagcagctg cagcccctcc
      121 cgttgcgggt cggcgagcag cgcctgcgat agctcgagca gggcctggtc ggcgggtgca
      181 acctggtaga agtcacagta ggcgcgcatc agcggcagca actctggcag gtcggcgggg
      241 ccgaccacgg tgatccgcac gggcactcct cctcacgccc aaaccggtgg gcaggaacgg
      301 ctcggcaccg ggtcgaagcg cacacctcca accttcagct tcagcagggg ggttgttccc
      361 cgcggaatgg ccgttcaccc gatcctttga accgcggcac gagcaagagc agcgcactga
      421 aggaagcgga ggcgggcaag ggcaacgcca ccgacgcccg gtccaggacg aatccggcgg
      481 gcgaccagcc acccacggcc acgccctggg tcaccccgga cgcctcgccg ggcccgacgg
      541 cagcctgacc tcgactactc gagcaggcgc ccggccacgc tcaggtccgg gtccggccgt
      601 gtccagccgg ccagctcggc cggcacggcc ggcacaggcc gggcgtcgac ggccaggcgg
      661 ccggtgccgt ccaggcgggc ctggaagttc acccgcagct cggggtcgag ctcgggatgg
      721 tcgcggcgct ggtgggcgcc gcgactctcg gtgcgggcca gcgcccccag cagggtggcc
      781 tcggcggcgg ccagggaggc gcgcaggtcc agggcgtggg ccaggtcgcc gtagccctcg
      841 gaggtcggcc ggacgtccac gtcggcggcc agcccccgca gctcggccag ccgctccagg
      901 ccacgctgga gcccagcctg gtcacggacc accccgcagg tctcccacat ggtgtcgcgg
      961 agcgcccgct gcagggggcg ggcgaactgg cgcccatagc ggatgaaacc ggacagctcc
     1021 tggtcggcga cccggaccac gccgcgggcc cgcagggcca catcacggga ggccgagtag
     1081 cgggcggcgg cctccccggc ccggcggcca aacacgaccg tctcggccag ggagttgccg
     1141 cccagccggt tggccccatg gagcccggcg gcgatctcgc ccgcggcgta caggccggcc
     1201 acgtcggtgg cgtgggtctc ggggtcgacc accaccccac ccatcgagta gtgggcggtc
     1261 ggggcgacct ccatcggctg ctgggagatg tccagcatct gcagctccag gaactgccgg
     1321 tacatccggg gcagcttctc caagatgacg tccttgccga ggtggctgat gtccaggaat
     1381 accccgccgt tggggcctcc ccggccctgg gcgatctcgg tgtagttggc cagcgccacc
     1441 cggtcgcggg ccgacagctc catccgctcg gggtcgtagc ggcccatgaa ccgctccccc
     1501 agcgcgttct tgagctggcc cccttcgccg cgcacggcct cagtgaccag ggtgcccgcc
     1561 gcctcctcgg gggcgaccat cccggtcggg tggaactgga ccagctccat gtcggccagc
     1621 cggcagccgg cccgcagggc caggtacatg ccgtcgccgg tgttctcgtc ccgccgcgag
     1681 gacgagcgcc gccagatgcg ggtgtggccg ccggcggcca acaccaccgc atcggccagg
     1741 aacacggtcc gcgcgccggt ctccaggtcg aaggccaacg ccccgaagca gaccccgtcg
     1801 gcgaccagca gctgcgacac gtactggtcg tcgatcacgg tcaggccgag ctcagcgacc
     1861 ttggcggcca gcgtccgcag gatcgcccgg cccgtccagt cgccggcata gcaggtccgc
     1921 cgccaccggt gggcgccgaa gaaccgctga tccagccgac cgtccggggt gcgggcgaac
     1981 ggggcgcccc agtcggccag ctccagcacc gccgccggcg cctccctggc cagcagctcg
     2041 accacccgcg ggtcggccag gaagtagccc tcccgcaggg tgtcgccgaa gtg
//
LOCUS       DASSWR010000469         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1089503, whole genome shotgun sequence.
ACCESSION   DASSWR010000469 DASSWR010000000
VERSION     DASSWR010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089503"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..873
                     /locus_tag="VFO47_07860"
     CDS             127..873
                     /locus_tag="VFO47_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8788648.1"
                     /translation="MSAGSPLLQMERVNKWFGDLHVLQDIDLEVDAGEVVIVVGPSGS
                     GKSTLCRTINRLEPIDGGDIRLEGRPLPSEGKALARLRSDVGMVFQQFNLFAHRSVLD
                     NVTLGPIKVRGQSRQQAEGRARELLARVGVENQADKLPAQLSGGQQQRVAIARALAMD
                     PKLLLFDEPTSALDPEMINEVLDVMRDLAAAGMTMIVISHEMGFARSAANRVVFMDDG
                     RILEEAQPEEFFASPRTERAKDFLSKIISH"
     gene            912..1793
                     /locus_tag="VFO47_07865"
     CDS             912..1793
                     /locus_tag="VFO47_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8788649.1"
                     /translation="MRTRRSTGLLGVVLALTLALAACGGDDEGGDGGGQVNEGASFAE
                     GTTMARLQQAGAVKVGTKFDQPLFGLKNLEGKPEGFDVEIAKLIAGEMGIAADKVEFI
                     ESVSANREPFIQQGKVDFVVATYTINDKRKQVVDFAGPYYVAGQDIMVAKGNPEGIAG
                     PNDLSGKKVCSVTGSTPAENIRTNYPKAELTEFDVYSKCAEALKNGQVQAVTTDNVIL
                     LGLIDQDPEAFELVGKPFTEEPYGIGLKKGDTEFRNFINDTLEKIYQDGRWKTAWEAT
                     AGKVATETPTPPQVDRY"
     gene            1818..2465
                     /locus_tag="VFO47_07870"
     CDS             1818..2465
                     /locus_tag="VFO47_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8788650.1"
                     /translation="MDAVLDNLDAYRQGFVTTLELSVLASVLALLLGTVLAAMRVSPV
                     PTLRAAGAAYVELVRNTPLTLVFFFVVFVLPQVDIKLPFFVFAVIALTIYHAAFFCEA
                     VRSGINAVGVGQAEAARSIGLTFGQSLQLVVLPQAIRTVIPPLINVVIALIKNSSIAA
                     AFAVVELTAIGTRLANANGDAVIPILAGVAVCYLVLTLPSGWLAGRLERRVAVLR"
     gene            2476..>3165
                     /locus_tag="VFO47_07875"
     CDS             2476..>3165
                     /locus_tag="VFO47_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8788651.1"
                     /translation="MSEAVLYDTVGPRGRRRILVGSIAGVLLVAVVLGAAVARLAAKG
                     GLDPELWRVLTQSAVQRLILRGLVATLQAAAIAMVLAMVVGAVLAIGRLSRRAWIAKP
                     AGAWVELFRGLPLLLLILFLFLGLPAVGVTISVFWALVAGLTLYNSAVLGEIFRAGIL
                     SLPKGQTEAAYSIGLRRGQTLQLILIPQAVRLMLPALISQLVTLLKDTSLGFVIGYAE
                     LLRNGRGAVEFL"
BASE COUNT          461 a         1127 c         1100 g          477 t
ORIGIN      
        1 ccggcctggg cgagcgctgg ctgtcggttc ccctcggcca gggaccacgg tgaggaagcg
       61 cacccccatc ccggcggggg ccactgtggt ttgataccgg aaagctgacc ggtgggaggc
      121 cagtggatgt cggcaggttc ccccctgctc cagatggagc gggtgaacaa gtggttcggc
      181 gacctgcacg tgctccagga catcgacctg gaggtggacg cgggcgaggt ggtgatcgtg
      241 gtcggcccgt cggggtcggg caagtcgacc ctttgccgga ccatcaaccg gctggagccg
      301 atcgacggcg gcgatatccg cctggagggc cggccgctgc ccagcgaggg caaggccctg
      361 gcccggctcc ggtccgacgt cggcatggtc ttccagcagt tcaacctgtt cgcccaccgg
      421 tcggtgctcg acaacgtcac cctcggcccg atcaaggtcc gcggccagtc ccggcagcag
      481 gccgagggcc gggcccggga gctgctggcc cgggtcgggg tcgagaacca ggccgacaag
      541 ctgcccgccc agctgtccgg tggccagcag cagcgggtgg ccatcgcccg cgccctggcc
      601 atggacccca agctgctgct gttcgacgag ccgacctcgg ccctcgaccc ggagatgatc
      661 aacgaggtcc tcgacgtcat gcgcgacctg gccgccgccg gcatgaccat gatcgtcatc
      721 agccacgaga tgggcttcgc ccgctccgcc gccaaccggg tcgtgttcat ggacgacggc
      781 cggatcctgg aggaggccca gcccgaggag ttcttcgcca gcccgcggac cgagcgggcc
      841 aaggacttcc tgtccaagat catcagccac tgacgggcgg ccccgaaccg cccaggcaag
      901 gaggaggcac catgcggacg cgacgctcga ccgggctgct gggggtcgtg ctggccctga
      961 cgctggccct ggccgcctgc ggcggcgacg acgagggcgg cgacggcggg ggccaggtca
     1021 acgagggggc cagcttcgcc gagggcacca ccatggcccg gctccagcag gccggcgcgg
     1081 tcaaggtcgg gaccaagttc gaccagcccc tgttcggcct caagaacctg gaaggcaagc
     1141 cggagggctt cgacgtcgag atcgccaagc tgatcgccgg cgagatgggc atcgcggccg
     1201 acaaggtcga gttcatcgag tccgtctcgg ccaaccggga gccgttcatc cagcagggca
     1261 aggtcgactt cgtggtcgcc acctacacca tcaacgacaa gcgcaagcaa gtggtcgact
     1321 tcgccggccc ctactacgtg gccggccagg acatcatggt cgccaagggc aaccccgagg
     1381 ggatcgccgg ccccaacgac ctgtcgggca agaaggtctg ctcggtcacc ggctcgaccc
     1441 cggccgagaa catccgcacc aactacccca aggccgagct gaccgagttc gacgtctact
     1501 ccaagtgcgc cgaggccctc aagaacggcc aggtccaggc cgtgaccacc gacaacgtca
     1561 tcctgctggg gctgatcgac caggacccgg aggcgttcga gctggtcggc aagccgttca
     1621 ccgaggagcc gtacggcatc gggctgaaga agggcgacac cgagttccgc aacttcatca
     1681 acgataccct ggagaagatc taccaggacg ggcgctggaa gacggcctgg gaggccaccg
     1741 ccggcaaggt ggccaccgag accccgaccc caccccaggt cgaccgctac tgaccgcagg
     1801 ggccggaggg tcgggacgtg gacgccgtcc tcgacaacct ggacgcctac cgccaggggt
     1861 tcgtcaccac cctggagctg tcggtgctgg cctcggtgct ggcgctgctg ctcggcacgg
     1921 tgctggcggc catgcgggtg tcccccgtcc cgaccctccg cgccgccggc gcggcctacg
     1981 tcgagctggt gcgcaacacg ccgctgacgc tggtcttctt cttcgtggtg ttcgtcctgc
     2041 cccaggtcga catcaagctg ccgttcttcg tgttcgcggt gatcgccctg accatctacc
     2101 acgccgcctt cttctgcgag gcggtccgct cgggcatcaa cgccgtcggg gtcggccagg
     2161 ccgaggcggc ccgctccatc ggcctcacct tcggccagtc gctccagctg gtggtcctgc
     2221 cccaggccat ccggacggtg atcccgccgc tgatcaacgt ggtcatcgcc ctgatcaaga
     2281 acagctcgat cgcggccgcc ttcgccgtgg tcgagctgac cgccatcggc acccggctgg
     2341 ccaacgccaa cggcgacgcg gtgatcccga tcctggccgg ggtggccgtc tgctacctgg
     2401 tcctgaccct gccgtcggga tggctggccg ggcggctgga gcgccgggtg gcggtcctcc
     2461 gatgaggagc ctccggtgag cgaggccgtc ctctacgaca ccgtcgggcc gcgggggcgg
     2521 cgccgcatcc tggtcggcag catcgccggg gtgctgctgg tggcggtcgt gctcggggcg
     2581 gcggtggccc gcctggccgc caagggcggc ctcgaccccg agctgtggcg ggtcctcacc
     2641 cagagcgcgg tgcagcggct aatcctgcgc ggcctggtcg ccaccctcca ggccgccgcg
     2701 atcgcgatgg tcctggccat ggtcgtgggg gccgtgctgg ccatcgggcg actgtcgcgg
     2761 cgggcctgga tcgccaagcc ggccggcgcc tgggtcgagc tgttccgcgg cctgcccctg
     2821 ctgctgctga tcctgttcct gttcctgggc ctgccggcgg tcggggtcac catctcggtg
     2881 ttctgggccc tggtggccgg cctcaccctc tacaacagcg ccgtgctcgg ggagatcttc
     2941 cgggccggga tcctgtcgct gcccaagggg cagaccgagg ccgcctactc gatcggtctc
     3001 cgccgcggcc agacgctgca gctcatcctg atcccccagg cggtccggct gatgctgccg
     3061 gccctcatca gccagctggt caccctgctc aaggacacct cgctggggtt cgtgatcggc
     3121 tacgccgagc tgctgcgcaa cggccgcggg gcggtggagt tcctc
//
LOCUS       DASSWR010000470         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_723150,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000470 DASSWR010000000
VERSION     DASSWR010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723150"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFO47_07880"
     CDS             complement(<1..278)
                     /locus_tag="VFO47_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788652.1"
                     /translation="MAALALLVVAVLAAGCTGGGDPEPAGEARAAAEGLTDLGRRLPQ
                     RIRTAREIRVGSDISYAPVEFYDAFAPDVLERPVGEPEPQVRGIDPDLA"
     gene            414..>2259
                     /locus_tag="VFO47_07885"
     CDS             414..>2259
                     /locus_tag="VFO47_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET8788653.1"
                     /translation="MNVKVGSMLGNLSLRPKIALILLLPVAALLLLAGLRIGSSVSTS
                     RQAAQVRGLTEFALRGTALAHELQRERGLTLGGLLAEQPSPGLREQRAATNRALAGYR
                     DSAADLDTDALPQAARDELASAGNRLQGLSGLRRDVNRGSVSPEAALDFYNAAIANLL
                     NTNRGLTSGIADPQLAQRVGAFVAVSRIKELAALERELVSDVLDRGRFAPGEFRRFTS
                     TLSTRAVLLSEFRAAADDAQRAVIVDTLVGPEVQRARELQAEAIASETTPELAIDLAE
                     WWTVNSTEINLLRRVEERLGAAAVDASRADESAARTRAAIDAAAVLLVLALAIGLSLV
                     VVRSLLRPLGLLRSSAEEVAHSQLPGVVERLQRAEPVDLNAETRPIGIRDRDEIGQVA
                     RAFDAVHSTAVRVAAEQAALRRSVADMFLSLGRRLQNLVHRQLELLDELERTEADPQQ
                     LRSLFRLDHLATRMRRNAENLLVLSGAEPVRRWSDPVPLARVIRAASAEIEDYNRVGV
                     MPLADVRVVGHAVSDVVHLLAELIENAAAFSPPGTRVQVAGEPAAHGYLLEIEDQGIG
                     MSDEELAEANEQLAKPATIDLASAQRLGFYVVGRLAARHGIKVRLRRSW"
BASE COUNT          287 a          884 c          802 g          286 t
ORIGIN      
        1 gccaggtcgg ggtcgatgcc gcggacctgg ggctcgggct cgccgacggg ccgctccagc
       61 acgtcggggg cgaaggcgtc gtagaactcc accggcgcat acgagatgtc ggagccgacc
      121 cggatctccc gggccgtgcg gatccgctgg ggcaggcgcc ggccgaggtc ggtcaggccc
      181 tcggcggcgg cccgggcctc gccggccggc tcgggatcgc cgccgccggt gcagccggcg
      241 gccagcacgg ccacgaccag cagggccagc gccgccacgg cgccgcgccg tcgtccaccg
      301 ccgcctaccc gcccttgcac catccaccgc acttgtgaca gcttgccttc ttcggagaca
      361 cgaagcctac tacggtccca ggtacgcatc gtgtcgatcg acccgtcatc cccatgaacg
      421 tgaaggttgg gtcgatgctc ggcaacctgt cccttcgtcc caagatcgcg ctgatcctcc
      481 tgctaccggt ggcggccctc ctgctgctgg ccgggctccg catcgggtcg agcgtctcga
      541 cgagccgtca ggccgcccag gtgcgcggcc tgaccgagtt cgccctgcgc ggcaccgccc
      601 tggcccacga gctgcagcgc gagcggggcc tgaccctcgg cggcctactg gccgagcagc
      661 cgtcgccggg cctgcgggag cagcgggccg ccaccaaccg cgccctggcc ggctaccgcg
      721 acagcgccgc cgacctcgac accgacgccc tcccccaggc cgccagggac gagctggcct
      781 cggccggcaa ccggctgcag gggctgagcg gcctgcgccg cgacgtcaac cggggctcgg
      841 tcagcccgga ggcggccctg gacttctaca acgccgccat cgccaacctg ctcaacacca
      901 accgcgggct gacctccggc atcgccgacc cgcagctggc ccagcgggtg ggggcgttcg
      961 tggccgtgtc ccggatcaag gagctggccg ccctcgagcg ggagctggtc tcggacgtgc
     1021 tggaccgagg ccgcttcgcc ccgggcgagt tccgccgctt cacctcgacc ctgtccaccc
     1081 gggcggtgct gctgtcggag ttccgggcgg ccgccgacga cgcccagcgg gccgtgatcg
     1141 tcgacaccct ggtcgggccc gaggtccagc gggcccggga gctccaggcg gaggccatcg
     1201 cctccgagac cacgcccgag ctcgcgatcg acctggccga gtggtggacg gtcaactcga
     1261 ccgagatcaa cctgctgcgc cgggtcgagg agcgcctggg cgcggccgcg gtcgacgcca
     1321 gccgggccga cgagtcggcc gcccgcaccc gggcggccat cgacgccgcc gccgtcctgc
     1381 tcgtcctggc cctggccatc ggcctgtccc tggtggtcgt ccggtcgctg ctgcggcccc
     1441 tgggcctgct gcggtcctcg gccgaggagg tcgcccacag ccagctgccc ggcgtggtcg
     1501 agcggctcca gcgggccgag ccggtcgacc tgaacgccga gacccggccc atcggcatcc
     1561 gcgaccgcga cgagatcggc caggtggcca gggcgttcga cgccgtccac tcgaccgcgg
     1621 tgcgggtcgc ggccgagcag gcggccctgc gccgcagcgt ggccgacatg ttcctcagcc
     1681 tcggccggcg cctccagaac ctggtccacc gccagctcga gctgctggac gagctggagc
     1741 gcaccgaggc cgacccccag cagctgcgca gcctgttccg cctcgaccac ctggccaccc
     1801 gcatgcgccg caacgccgag aacctgctcg tcctgtccgg cgccgaaccg gtgcggcgct
     1861 ggagcgaccc ggtgccgctg gcccgggtga tccgcgccgc ctcggccgag atcgaggact
     1921 acaaccgggt cggggtcatg cccctggccg acgtgcgggt cgtcggccac gcggtcagcg
     1981 acgtcgtcca cctgctggcc gagctgatcg agaacgcggc cgcgttctcg ccccccggga
     2041 ccagggtcca ggtggcgggc gagccggccg cccacggcta cctgctggag atcgaggacc
     2101 agggcatcgg catgtccgac gaggagctgg ccgaggccaa cgagcagctg gccaagccgg
     2161 ccacgatcga cctggccagc gcccagcggc tcgggttcta cgtggtcggc cgcctggccg
     2221 cccgtcacgg catcaaggtg cggctgcgcc gctcgtggt
//
LOCUS       DASSWR010000471         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_611171,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000471 DASSWR010000000
VERSION     DASSWR010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611171"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFO47_07890"
     CDS             <1..219
                     /locus_tag="VFO47_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daunorubicin/doxorubicin resistance ABC
                     transporter ATP-binding protein DrrA"
                     /protein_id="HET8788654.1"
                     /translation="GAEPEVDPDTRRVSAPVRDRVAALTEAARELDAAGVAVEDVALR
                     RPTLDEVFLHLTGRPAETPEERPQEVPA"
     gene            216..1061
                     /locus_tag="VFO47_07895"
     CDS             216..1061
                     /locus_tag="VFO47_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788655.1"
                     /translation="MTVPTVQSPQAPPVPETPLQRLRWSLADAWTVTGRDLAHWVRQP
                     ATLVLNLLFMVMVVLLFGYLFGGAMTVPGGGSYREFLLPGMFALSMLFGVEATMVAVT
                     TDAARGVTDRFRAMPMARSAVAGGRAIADMLGSTLGLAVLLACGLLVGWGWHGSATDS
                     LAAVGLLLLLRFAFVWVGIYLGLLVRNPEAVVAVQVLVWPIGFLSSAIAPTATMPGWL
                     GALAEWNPVSATASATRELFGNPGWGGASWADQHALALAVAWPLLILAVFVPLAVRRW
                     QRLSH"
     gene            complement(1003..1848)
                     /locus_tag="VFO47_07900"
     CDS             complement(1003..1848)
                     /locus_tag="VFO47_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8788656.1"
                     /translation="MGADRFGVRSADGAPLAVWVEGQGPPLVLVHGSMCDHTASDPLV
                     AELRDTMTTFAMDRRGFGASGDAAGYALEREFEDVAAVVEAVAARTGGPVALWGHSYG
                     AGCAMGGAALTGAVHHLVLYEPGLGIPYPAGSIEEVEAALAADDAETALLLVLAGVVG
                     MTDEEIASLRSGPRWPTMLASAPTVPRECRAEDGWTYRPGAFDGLSAPTLLLAGTESH
                     PVLQQATDRALAAIPGARVQVLEGHAHLAHRSDPAMVAAVIRGFVSGSAAASGARPGA
                     RTRPG"
     gene            complement(1848..2678)
                     /locus_tag="VFO47_07905"
     CDS             complement(1848..2678)
                     /locus_tag="VFO47_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788657.1"
                     /translation="MARTTEATLTRTVSRDGTEIGWWTSGEGPPLVLVHGAVADHTRW
                     RPLLPYLEPHATVHAMDRRGRGASGDAPGYDLAREFEDVAAVVDAVAAVSESAVDLYG
                     HSFGGLCAFGGATLTANVRRLVLYEGWPPADPAARELPPDVGQRLDALLAEGDHDAVV
                     ETMFREVVRMPEADLAALRAQPAWTARVAAAPALVRELRAISRVAFDPALAARITVPA
                     LLLTGSDSRDPFAADTRTVAAALPDARVVVLEGQQHVADILDPEAFAGHLVGFLRGRR
                     "
     gene            complement(2746..>3475)
                     /locus_tag="VFO47_07910"
     CDS             complement(2746..>3475)
                     /locus_tag="VFO47_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET8788658.1"
                     /translation="HGHSSGVAALARGAGERLRLPPATVADLEVAGLLHDVGRVAVPD
                     TVGEKPGGLSGHEWEQVRLHAYHSERILAGSDRLASLAPMVGMHHERLDGGGYHRGST
                     RAELPVPARVLAAADAYQAMTQARPHRPALVPEEAERRLLAGARSGALDPDAAAAVLA
                     VAGHAPVVPRQARPAGLSAREVEVLGLVAQGCSNAQIAERLVISRRTAEHHVQHIYTK
                     IGVSSRAAAALFAMEHHLLAPGDR"
BASE COUNT          382 a         1345 c         1323 g          425 t
ORIGIN      
        1 ggggccgagc ccgaggtcga ccccgacacc cgccgagtca gcgcgccggt ccgcgaccgg
       61 gtggccgcgc tcaccgaggc ggcccgcgag ctggacgcgg ccggcgtcgc cgtcgaggac
      121 gtcgccctgc gccgcccgac cctggacgag gtgttcctcc acctcaccgg gcgccccgcc
      181 gagacgcccg aggagcgacc ccaggaggtg ccggcatgac cgtgccgacc gtgcagtccc
      241 cgcaggcccc gcccgtgccc gagacgcccc tccagcgcct gcgctggtcg ctggccgacg
      301 cctggaccgt gaccggccgc gacctggccc actgggtccg ccagccggcc accctggtcc
      361 tgaacctgct cttcatggtc atggtggtcc tgctgttcgg ctacctgttc ggcggggcca
      421 tgacggtgcc gggcggcggc agctaccggg agttcctcct gcccggcatg ttcgccctca
      481 gcatgctctt cggcgtcgag gcgaccatgg tcgccgtcac caccgacgcc gcccggggcg
      541 tcaccgaccg gttccgggcc atgcccatgg cccgctcggc cgtggccggc ggccgcgcca
      601 tcgccgacat gctcggctcc accctcggcc tggccgtgct gctcgcctgc gggctgctgg
      661 tcggctgggg ctggcacggg agcgccaccg actccctggc cgcggtcggg ctcctgctcc
      721 tgctccggtt cgccttcgtc tgggtgggga tctacctggg cctgctcgtc aggaacccgg
      781 aggcggtcgt ggccgtgcag gtcctggtgt ggccgatcgg cttcctcagc agcgccatcg
      841 cccccaccgc caccatgccg ggctggctcg gcgccctggc cgagtggaac cccgtgtcgg
      901 ccaccgcctc cgcgacccgc gagctgttcg gcaaccccgg ctggggcggc gcctcctggg
      961 ccgaccagca cgccctggcc ctggcggtgg cctggccgct gctcatcctg gccgtgttcg
     1021 tgcccctggc cgtgcgccgc tggcagcggc tgagccactg acgaaccccc ggatcacggc
     1081 cgcgaccatg gccgggtcgc tgcggtgggc gaggtgggcg tggccctcga gcacctggac
     1141 ccgggcgccg gggatggccg ccagcgcccg gtcggtcgcc tgctgcagca cgggatggct
     1201 ctcggtgccg gccagcagga gggtcggcgc cgagagcccg tcgaaggcgc cgggccggta
     1261 ggtccagccg tcctcggccc ggcactccct gggcacggtg ggggcgctgg cgagcatcgt
     1321 cggccagcgc gggcccgagc gcagggaggc gatctcctcg tcggtcatcc cgacgacgcc
     1381 ggccagcacc agcagcagcg ccgtctcggc gtcgtcggcg gccagcgcgg cctcgacctc
     1441 ctcgatcgag ccggcgggat aggggatgcc gagcccgggc tcgtagagga cgaggtggtg
     1501 gacggcgcct gtcagggcgg ccccgcccat ggcgcagccg gccccgtagg agtggcccca
     1561 gagcgccacc ggcccgccgg tccgggccgc gaccgcctcg accacggcgg ccacgtcctc
     1621 gaactcccgc tccagcgcgt agccggccgc gtccccgctg gcgccgaagc cccggcggtc
     1681 catggcgaag gtcgtcatgg tgtcccgcag ttcggcgacc agcgggtcgg acgccgtgtg
     1741 gtcgcacatg gaaccgtgca ccagcaccag cggcggcccc tggccctcga cccagacggc
     1801 cagcggggcc ccgtcggccg accggacgcc gaagcggtcc gcgcccatca gcgccgcccc
     1861 cgcaggaacc cgaccaggtg gccggcgaac gcctcggggt cgaggatgtc cgcgacgtgc
     1921 tgctggccct ccaggaccac gacccgggcg tcgggcaggg cggcggccac ggtccgggtg
     1981 tcggcggcga acgggtcgcg gctgtccgag ccggtcagca gcagcgccgg cacggtgatc
     2041 cgcgccgcca gcgcgggatc gaaggccacc cgggagatgg cccggagctc gcggacgagt
     2101 gccggcgcgg ccgcgacccg cgccgtccag gccggctggg cccggagggc ggcgaggtcc
     2161 gcctcgggca tccgcacgac ctcgcggaac atggtctcca cgaccgcgtc gtggtcgccc
     2221 tcggccagca gggcgtccag ccgctgtccg acgtccgggg ggagctcgcg ggcggcgggg
     2281 tcggcgggcg gccagccctc gtagaggacg agccgccgga cgttggcggt cagcgtcgcc
     2341 ccgccgaacg cgcacagccc gccgaaggag tggccgtaca ggtcgaccgc ggactccgag
     2401 accgcggcga ccgcgtcgac cacggcggcg acgtcctcga actcccgggc caggtcgtag
     2461 ccgggggcgt cgccgctggc gccgcggcca cgccggtcca tggcgtggac ggttgcgtgg
     2521 ggctccagat agggcagcag cgggcgccag cgcgtgtggt cggccaccgc cccgtgcacc
     2581 agcaccaggg gcggcccctc gccgctggtc caccagccga tctcggtgcc gtcgcgggac
     2641 accgtccgtg tcagggtcgc ctcggtggtt ctggccatgg ctcctgctcc tctcgtcgag
     2701 tggagctcag cctcgcccgg cggccggggg gccgcatcgg tagggctacc tatcccccgg
     2761 tgccagcagg tggtgctcca tcgcgaacag ggccgccgcg gcccggctgg acacgccgat
     2821 cttggtgtag atgtgctgca cgtggtgctc ggcggtgcgc ctggagatga ccaggcgctc
     2881 ggcgatctgg gcgttgctgc acccctgcgc gaccagtccc agcacctcca cctcgcgggc
     2941 gctcagcccg gcgggccggg cctggcgggg gaccacgggg gcgtggccgg ccacggccag
     3001 cacggcggcg gcggcgtcgg ggtcgagggc tccggagcgg gcgccggcga gcagccgccg
     3061 ctccgcctcc tccgggacca gcgcgggccg gtgcggccgg gcctgggtca tcgcctggta
     3121 ggcgtcggcg gccgccagca cccgggccgg cacgggaagc tcggcccggg tggagccgcg
     3181 gtggtagccg ccgccgtcga gccgctcgtg gtgcatcccg accatggggg ccagcgacgc
     3241 cagccggtcc gacccggcga ggatccgctc ggagtggtag gcgtgcagcc ggacctgctc
     3301 ccactcgtgc ccgctcaggc cgcccggctt ctcccccacc gtgtccggga cggccacccg
     3361 gccgacgtcg tggagcagcc cggcgacctc gaggtcggcc acggtggccg gcgggaggcg
     3421 cagccgctcg ccggcgccgc gggccagggc ggccacgccg ctggaatgcc cgtgg
//
LOCUS       DASSWR010000472         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_639971,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000472 DASSWR010000000
VERSION     DASSWR010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639971"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFO47_07915"
     CDS             complement(<1..801)
                     /locus_tag="VFO47_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET8788659.1"
                     /translation="MRDIHGLRVPDRLYQAYAFDLDGTIYLGDHLLPGARRLVEELRR
                     RGLVVRFLSNNPTKDPEQYADKLARLGLPTPVDEIVNTVVTMTRWLLDNAPGAVVYPI
                     SEPPLVRALEKAGIRMSSDPAEIDVVVASYDRTFTYEKLQIAFDAIWYHRRARLVATN
                     PDRFCPFPGGRGEPDCAAIIAAVEACTGVRCEANTGKPDPAMLRAALTGLDVAPADCV
                     MVGDRLSTDIRMGLDTGMAAALVLTGDTAAEDLQELPGDDAPGIVVDRI"
     gene            complement(807..1454)
                     /locus_tag="VFO47_07920"
     CDS             complement(807..1454)
                     /locus_tag="VFO47_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788660.1"
                     /translation="MTTQIPLSRRRVLDAAVALADRDGVGALSMRKLAQELGVEAMSL
                     YHHVAGKDAILDGIVDVVFAEIELPSGDGGWKAAMRRRAVSAREVLRRHPWATALMES
                     RSTPGPANLRHHDAVLGVLRNAGFSVELAAHAYSLLDAYIYGFALQESSLPFDTPEET
                     AEVASSIMDGFPADAFPYLTEIAVEHVLQPGYDYGNEYRFGLELILDGLERALSG"
     gene            1596..2573
                     /locus_tag="VFO47_07925"
     CDS             1596..2573
                     /locus_tag="VFO47_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET8788661.1"
                     /translation="MRAIVQDRYGGPDVLELREVGRPAPKDDEVLIRVRAAGLHRGDW
                     HVMTGLPYLIRLVVPTLGLRRPKTPVLGMDVAGTVEAVGGNVTRFQPGDAVFGWTDGA
                     FAEYATAPEDQLAPMPATLGFEQAAVVPVSGFAALQALRDVGEIQAGQRVLVIGAAGG
                     VGSFAVRLAKAFGTEVTGVGSTTQLDLIRSLGAGHVIDYTREDVTDGTRHWDLILDTA
                     GHRSLSRLRRALTPAGTLVIVGSEGRGRWLGGFDRNLRAVVLSRFVGQRLRMLSSTPR
                     PRDLQTLRELVEAGKLTPVVDRTYPLAEVPDAIRHMVAGHVRGKLVITV"
     gene            2693..3220
                     /locus_tag="VFO47_07930"
     CDS             2693..3220
                     /locus_tag="VFO47_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788662.1"
                     /translation="MSRSTIPAWLATAGGMTVLVAATLWIPTVVTVATGLAVLALSAL
                     VLYQAATRTGTGGGHGLAGLVTAALGLLLVGLLGMPGVASDTSALDLRWAPPAGAEPA
                     PPTPEPAPPTEPQPDPGQQPQPGPLHPAEVEASAWAGPSKDGAGSTVTFEAENLVDGL
                     ADTAWRVEGDGVGTP"
     gene            3241..3525
                     /locus_tag="VFO47_07935"
     CDS             3241..3525
                     /locus_tag="VFO47_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788663.1"
                     /translation="MHLTAIGLVPGYAKVDQVSGVNRFRQNRRVTAATYTFDGGPQVA
                     VTFSDTAELQLVEVDADTTTVVIEITGSTRATERPYTAISEVQFAGWPIG"
     gene            3539..>4027
                     /locus_tag="VFO47_07940"
     CDS             3539..>4027
                     /locus_tag="VFO47_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788664.1"
                     /translation="MTTGRPRSTETTRYLCAAAYLHRRFADDVITKVLEEEHRAVAPS
                     YGVDIVPVVRHCLTAQRRRLTRDAVLTGLLLVGGPLLLLAGGSLPGVGLRLAVLAWAV
                     IFVESCINRYEALVRLLRNRFNPDAAQPPLSRRQARLLRELERNEAGNVTVYSGFSPF
                     VGC"
BASE COUNT          556 a         1449 c         1474 g          548 t
ORIGIN      
        1 gatgcgatcg accacgatgc cgggcgcgtc gtctccgggc agctcctgca ggtcctccgc
       61 ggccgtgtcc ccggtgagga cgagggcggc cgccatgccg gtgtcgagac ccatgcggat
      121 gtcggtcgac agccggtccc ccaccatgac gcagtcggcg ggggcgacgt cgagcccggt
      181 cagggcggcc cgcagcatcg ccgggtccgg cttgccggtg ttggcctcgc agcggacgcc
      241 ggtgcaggcc tcgacggcgg cgatgatggc cgcgcagtcc ggctccccgc ggccgccggg
      301 gaacgggcag aaccggtccg ggttggtggc gaccagccgg gcccgccggt ggtaccagat
      361 ggcgtcgaag gcgatctgca gcttctcgta ggtgaaggtc cggtcgtagc tggccacgac
      421 gacgtcgatc tcggccgggt cgctggacat gcggatgccc gccttctcca gcgcccggac
      481 cagcggcggc tcgctgatcg ggtagacgac ggcgccgggg gcgttgtcga gcagccaccg
      541 ggtcatggtc acgaccgtgt tgacgatctc gtccaccggg gtcggcagcc ccagccgggc
      601 cagcttgtcg gcgtactgct ccgggtcctt ggtcgggttg ttggaaagga accggaccac
      661 cagcccccgc cggcgcagct cctcgaccag ccgcctggct cccggcagca ggtggtcccc
      721 gaggtagatg gtgccgtcga ggtcgaacgc gtacgcctgg tagagccggt ccggcacccg
      781 caggccgtga atgtccctca tggccgctag cctgacaggg cccgctcgag gccgtcgagg
      841 atcagctcga gcccgaaccg gtactcgttc ccgtagtcgt agccgggctg caggacgtgc
      901 tcgacggcga tctcggtgag gtacgggaag gcgtcggccg ggaagccgtc catgatcgag
      961 gaggccacct cggcggtctc ctccggcgtg tcaaagggca ggctcgactc ctgcagggcg
     1021 aacccgtaga tgtaggcgtc gagcagcgag taggcgtggg cggccagctc gacggagaag
     1081 ccggcgttcc ggaggacgcc gaggacggcg tcgtggtggc gcaggttggc ggggccgggc
     1141 gtggaccgcg actccatcag ggcggtggcc caggggtggc gccggagcac ctcccgggcc
     1201 gagacggccc gccggcgcat ggccgccttc cacccgccgt cgcccgacgg cagctcgatc
     1261 tcggcgaaga cgacgtcgac gatgccgtcc aggatggcgt ccttgccggc cacgtggtgg
     1321 tacagcgaca tggcctcgac cccgagctcc tgggcgagct tgcgcatgct gagcgcccca
     1381 acgccgtccc ggtcggcgag ggcgacggcg gcgtcgagga cccgccgccg gctcaggggg
     1441 atctgggtgg tcatctcgaa tcctcaatgc aggggttgac gaacttacac tgtaagtcta
     1501 ccttacatcg taaggcttcc gctgagaagg gaacgaagat ggacacgacc accgtcggca
     1561 aggacgccgg gacggccacc aaccagggaa gcaccatgcg ggcgatcgtc caggaccgct
     1621 acggcggccc ggacgtgctg gagctccggg aggtcggcag gccggcgccc aaggacgacg
     1681 aggtgctgat ccgggtgcgg gcggccggcc tccaccgggg cgactggcac gtcatgaccg
     1741 gcctgcccta cctgatccgc ctcgtggtcc cgacgctcgg gctgcgcagg cccaagaccc
     1801 ccgtcctggg catggacgtc gccgggaccg tcgaggcggt cggcggcaac gtcacccggt
     1861 tccagcccgg cgacgcggtg ttcggctgga ccgacggcgc cttcgccgag tacgccaccg
     1921 cacccgagga ccagctggcg cccatgccgg ccaccctcgg cttcgagcag gccgcggtcg
     1981 tgcccgtctc cgggttcgcc gcactccagg cgctccggga cgttggcgag atccaggccg
     2041 gccagcgggt cctggtcatc ggggcggccg gcggcgtcgg gtcgttcgcc gtgcggctgg
     2101 ccaaggcgtt cgggaccgag gtcaccgggg tgggcagcac gacccagctc gacctcatcc
     2161 gctccctcgg cgccggccac gtgatcgact acacccgcga ggacgtcacc gacgggacgc
     2221 gccactggga cctgatcctc gacaccgctg gccaccgttc gctgtcccgg ctccggcgtg
     2281 ccctcacccc ggcggggacg ctggtcatcg tcggcagcga agggcgagga cggtggctgg
     2341 gaggcttcga ccgcaacctc cgggcggtgg tgctgtcacg gttcgtgggc cagcgcctgc
     2401 ggatgctgag ctccacgcca cgcccgcggg acctccagac cctgcgcgag ctcgtcgagg
     2461 ccggcaagct caccccggtg gtcgaccgga cctacccgct ggccgaggtc cccgacgcca
     2521 tccggcacat ggtggctggc cacgtccggg gcaagctcgt catcaccgtg tgagccggcg
     2581 gcccggcctg tgtcgggtat cacatctcgg ccgtaccacg cggcactgac aatccgtcgg
     2641 atctcgacat gctgggggtt gcacggcgac acgcgcgaat gaggaggggg cgatgtcgcg
     2701 gtcaacgatc ccggcctggc tggccaccgc ggggggcatg acggtgctgg tcgccgcgac
     2761 cctgtggatc ccgacggtgg tgacggtcgc gacggggctg gcggtgctcg cgctcagcgc
     2821 gctggtgctg taccaggcgg cgaccaggac gggcaccggt ggcggccacg ggctggccgg
     2881 cctcgtcacc gccgccctcg ggctgctcct ggtcgggctc ctgggcatgc ccggcgtcgc
     2941 cagcgacacg agcgcgctcg acctcaggtg ggccccgccg gctggcgccg aaccggcgcc
     3001 acccaccccc gagccggcgc cgcccaccga gccgcagccg gacccgggcc agcagccaca
     3061 gccgggcccg ctccacccgg ccgaggtcga agcgtcggcg tgggccggtc ccagcaagga
     3121 cggcgccggc tccacggtga cgttcgaggc cgagaacctg gtcgacgggc tggccgacac
     3181 cgcctggcgg gtggagggcg acggcgtcgg cacaccctga cggtccgctt cgaccgcccg
     3241 gtgcacctga ccgcgatcgg gctggtgcca ggctacgcca aggtcgacca ggtgagcggc
     3301 gtgaaccggt tccggcagaa ccggcgggtc acggccgcga cctacacctt cgacggcggc
     3361 ccccaggtcg ccgtcacctt ctccgacacc gccgagctgc agctggtcga ggtcgacgcg
     3421 gacaccacca cggtggtgat cgagatcacc ggttccacca gggcgacgga gcgcccctac
     3481 acggcgatct ccgaggtgca gttcgccggc tggccgatcg gctgaaaggc ggcagacgat
     3541 gacgaccgga cgaccgcgat cgaccgagac gacccgctac ctgtgcgccg ccgcctacct
     3601 ccaccggcgg ttcgcggacg acgtcatcac caaggtcctg gaggaggagc accgcgccgt
     3661 cgccccctcg tacggcgtgg acatcgtccc cgtggtccgt cactgcctga cggcccagcg
     3721 ccggcgcctg acccgggacg ccgtgctcac cgggctgctg ctggtcggcg ggccgctgct
     3781 cctgctcgcc ggcgggtcgc tgcccggcgt cggcctgcgc ctggccgtcc tggcctgggc
     3841 ggtcatcttc gtggagtcct gcatcaaccg ctacgaggcg ctggtccggc tgctgcggaa
     3901 ccgcttcaac ccggacgccg cccagccgcc gctcagccgc cgccaggcca ggctgctccg
     3961 cgagctggaa cgcaacgagg ccggcaacgt gaccgtctac agcgggttct cgccgttcgt
     4021 gggctgc
//
LOCUS       DASSWR010000473         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_921639,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000473 DASSWR010000000
VERSION     DASSWR010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921639"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFO47_07945"
     CDS             <1..1089
                     /locus_tag="VFO47_07945"
                     /inference="COORDINATES: protein motif:HMM:NF027896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8788665.1"
                     /translation="DVAALAEAVRAAPRGVLVAGWGADLDAGAADAFAAASGWPVLAD
                     PLSGARRGPAAVSTYDGLLRAPRFAAAHRPDLAVRVGGAPTSKALTAWLDASVPQVLV
                     DPAGGWLDPARTAALRLTADPSALLAATAARLAAGPSDRWLRSWLDAERLAREAIDGL
                     LDEWAEPFEGRVARDLAGWVPEGGTLVVGSSMPVRDVDAFARARDGLRFVANRGLSGI
                     DGFVATALGVAAAGEEPVAALCGDLSLLHDASSLLGAAGRPRGAVLVVCDNDGGGIFS
                     FLPQAQLPGELFEPLFGTPHGLDLTALAAAARVPCRVVEKAADLVPALDAALAGGGTR
                     LVLVRSERAANLARHRALAEAVAAAVGG"
     gene            1099..1983
                     /locus_tag="VFO47_07950"
     CDS             1099..1983
                     /locus_tag="VFO47_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788666.1"
                     /translation="MLFEGFDSRTVAAEGAAIHLRLGGHGPPLLLLHGYPQTSAMWHK
                     VAPGLAERFTVVAADLRGYGRSGKPPSDPGHLAYSKRVMARDMIEVMAALGFGAFGVA
                     GHDRGGRVGYRMALDHPEVVTRLAVLDIVPTHVMWQRMDRELAMATYHWLFLAQPDGL
                     PETLIGADPGWWVREKLRRWAGDPAAFDPEAVEEYVRCFADPAAVHASCEDYRAGATI
                     DDRLDADDLGRRRIACPVLALWGIDGIARLAEDPLVSWRPWCDDVRGQGVPGGHFLPE
                     ESPAETLAALDGFFRPPG"
BASE COUNT          187 a          815 c          756 g          252 t
ORIGIN      
        1 gacgtggccg ccctggccga ggcggtccgg gccgcccccc gcggggtgct ggtggccggc
       61 tggggcgcgg acctggacgc gggcgccgcc gacgccttcg ccgccgccag cggctggccg
      121 gtcctggccg acccgctgtc cggggcccgc cgcggcccgg cggccgtctc cacctacgac
      181 ggcctgctcc gggcgccccg gttcgccgcc gcccaccgcc ccgacctggc cgtccgggtc
      241 ggcggcgccc ccaccagcaa ggccctgacg gcctggctgg acgcgtccgt gccccaggtc
      301 ctggtcgacc cggccggcgg ctggctcgac cccgcccgca ccgccgccct gcgcctcacc
      361 gccgacccct ccgccctcct ggccgccacc gccgcccggc tcgccgccgg cccctccgat
      421 cggtggctcc ggtcctggct ggacgccgag cggctggccc gggaggcgat cgacgggctg
      481 ctggacgagt gggcggagcc gttcgagggc cgggtggccc gcgacctggc cggctgggtc
      541 ccggaggggg ggacgctggt ggtcggctcc agcatgccgg tccgggacgt ggacgccttc
      601 gcccgggcgc gggacggcct gcggttcgtg gccaaccgcg ggctgtcggg gatcgacggg
      661 ttcgtggcca ccgccctggg ggtggcggcg gccggcgagg agccggtggc ggccctctgc
      721 ggcgacctgt cgctgctgca cgacgcctcc agcctgctcg gcgcggccgg gcggccgcgg
      781 ggggcggtcc tggtcgtgtg cgacaacgac ggcggcggga tcttcagctt cctcccccag
      841 gcccagctcc ccggcgagct gttcgagccg ctgttcggca ccccccacgg gctggacctg
      901 accgccctgg ccgccgccgc gcgggtcccc tgccgggtgg tcgagaaggc ggccgacctg
      961 gtcccggccc tcgacgccgc cctggccggc ggcggcaccc ggctggtgct ggtgcgcagc
     1021 gagcgggcgg ccaacctggc ccgccaccgc gccctggccg aggccgtggc cgccgccgtc
     1081 ggcgggtagg ctgccgccat gctgttcgag gggttcgaca gccggaccgt ggccgccgag
     1141 ggggccgcga tccacctgcg cctcggcggc cacggcccgc cgctgctgct gctccacggc
     1201 tacccccaga cctcggccat gtggcacaag gtcgcgcccg ggctggccga gcggttcacc
     1261 gtggtcgcgg ccgacctgcg cggctacggc cgctccggca agccgccctc cgaccccggc
     1321 cacctcgcct actccaagcg ggtcatggcc cgcgacatga tcgaggtcat ggccgccctc
     1381 ggcttcggcg ccttcggggt cgccggccac gaccggggcg gccgggtcgg ctaccgcatg
     1441 gccctcgacc acccggaggt ggtgacccgg ctggccgtgc tcgacatcgt cccgacccat
     1501 gtgatgtggc agcggatgga ccgggagctg gccatggcca cctaccactg gctgttcctg
     1561 gcccagcccg acggcctccc cgagaccctg atcggcgccg accccggctg gtgggtgcgg
     1621 gagaagctgc gccgctgggc cggcgacccg gcggcgttcg accccgaggc ggtcgaggag
     1681 tacgtgcgct gcttcgccga cccggccgcc gtccacgcct cctgcgagga ctaccgggcg
     1741 ggcgcgacca tcgacgaccg gctggacgcc gacgacctgg gccggcgccg gatcgcctgc
     1801 ccggtcctgg ccctctgggg catcgacggc atcgcccgcc tggccgagga cccgctggtc
     1861 tcctggcggc cctggtgtga cgacgtccgc ggccagggcg tccccggcgg ccacttcctg
     1921 cccgaggagt ccccggccga gaccctggcc gccctggacg ggttcttccg gccgccgggc
     1981 taatgccggc accagaacta cgcagtatcc
//
LOCUS       DASSWR010000474         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1147830, whole genome shotgun sequence.
ACCESSION   DASSWR010000474 DASSWR010000000
VERSION     DASSWR010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147830"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="tal"
                     /locus_tag="VFO47_07955"
     CDS             complement(<1..1023)
                     /gene="tal"
                     /locus_tag="VFO47_07955"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET8788667.1"
                     /translation="MTTTNPLVQLSDQGVSVWVDSIARDWLEKGELRRLRDDFSVVGV
                     TSNPTIFQAAIGAGGFYDDQLAGLAEQGLGAQAIFERLALYDIRWAAHELRPVYEATG
                     GRDGYVSYEVPPDLAHDTDETIFQAARLFDLLSLPNVMIKIPATVEGLPAIQASIAAG
                     VNVNVTLIFSIARYREVVEAYLAGLEDRAAKSGDLGRVASVASFFVSRVDTLIDPQLE
                     ERVEEGGPDAAPAEARLGTAAIDNAKLAYEAWLELFSGPRWEALESKGARPQRCLWAS
                     TSTKNPDYRDVLYVEELIATDTVNTMPLSSVEAFADHGIVRGPTVLENLDRARRLWSD
                     LIRVGID"
     gene            complement(1043..3118)
                     /gene="tkt"
                     /locus_tag="VFO47_07960"
     CDS             complement(1043..3118)
                     /gene="tkt"
                     /locus_tag="VFO47_07960"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET8788668.1"
                     /translation="MTIQTAPTVPATDLDRLAVDTVRTLAMDTIQKAGSGHPGTAMAL
                     APAAYVLWTRFLKFDPADPEWFDRDRFVLSNGHASVLQYAVLHLTGYPLGIRDLKQQR
                     QWGSLTPGHPEYGHTPGVEVTTGPLGQGVGNGVGFAIAERLLAARYNRPGQEVVDHRT
                     WVFCGDGDMMEGVSSEASSLAGHLRLGKLCVLYDDNRITIDGPTSITFTEDVEARYRA
                     YGWHTLAVEDANDLEAIAAAYQAAVEETERPSFIRLRSVIAWGAPNAQDTSKAHGAAL
                     GEDEVRATKEVYGWNPDKHFKVPEGVPERWQVRVATNREERSAWQGRLRAFSDAEPEL
                     AAELRAALAGELPDGWDAELDNLLAEPRKQATRKASQQAINAIAANLPALVGGSADLA
                     ESNLTDIAGGGDLTATEAGRNLRFGIREHGMGAIANGLAAHGGLRPFCATFLVFSDYM
                     RPSVRLAALMGLPVVYVWTHDSIGLGGDGPTHQPVEHLGSLRLIPNLWVMRPADGPET
                     AEAWRAALQRTDGPTALVLSRQELPPVDRNRYASAAGLHRGAYVLAEAGGGAPELILL
                     ATGSEVWLALAAQDELESRQEIPTRVVSMPCWELFEAQPLDYRDEVLPPQVTARLAVE
                     AGSTPLWRQWVGLGGGVVGVDRFGASAPGGTALIKYGFTEDNMVERALAVLHRTHIEK
                     ARMLRNLSA"
     gene            complement(3115..4671)
                     /locus_tag="VFO47_07965"
     CDS             complement(3115..4671)
                     /locus_tag="VFO47_07965"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET8788669.1"
                     /translation="MPDPGLIIGVDIGTTGVKAAAVDAAAVSHGGAEREYPTESPFPG
                     WSVQDPDVVAAASAAVVREVTARAGAAGRPIAGVAFSGAMHSLIALALDGSPLTPSVT
                     WADSRAAAQARRLRDSGEWLALHRRTGTPVHPMSPLVKLLWYREQQPEVWHRAAHWVG
                     IKEYVLSRLAGLLAVDESIASATGLYGLAARDWDTTALDLAGLRREQLPALHATTDVV
                     ARLGADVAAWGLQAGTPIVVGASDGVLANLGVNAVAPGVAACSIGTSAAIRGVVRAPS
                     VDDQGRVFCYVLVPGRWVVGGATNNGGNVLRWLARVAGADLPDPDAGLTELAATVPPG
                     AEGLLMLPYLSGERAPRWAGDPYGAFLGLTQRHTRAHLARAALEGVCLQLALVLEAMQ
                     AAGLELAELRATGGFARSPFWRQLLADVLGTPIGFPVSEQGSAAGAALLGHVALGHLT
                     SVDDAAALVRVTEVVHPDRDAARFYRRLLPAFARTAEAVEDLNAWRADEAPDGLTHAA
                     TGQASKEEFP"
     gene            complement(4706..>5206)
                     /locus_tag="VFO47_07970"
     CDS             complement(4706..>5206)
                     /locus_tag="VFO47_07970"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579819.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET8788670.1"
                     /translation="GPVDDPKQLVEDVRAALWASKVVAYAQGLDQLGAASEAYRWGVD
                     VATVAKIWRAGCIIRARLLERIRQAYAIADLPTLLADPDVAEALGQAQDGWRRVVSLA
                     ASQGVPVPGFASALAYYDTVRRDRLPAALVQGLRDFFGAHTYRRVDREGVFHTLWSED
                     RTEQEV"
BASE COUNT          678 a         1938 c         1898 g          692 t
ORIGIN      
        1 gtcgatgccg acccggatca ggtccgacca cagccgccgg gcccggtcca gattctccag
       61 cacggtcggg ccgcggacga tgccgtgatc ggcgaacgcc tccacgctgg acaggggcat
      121 ggtgttgacc gtgtcggtgg cgatcagctc ctccacgtac aggacgtcgc ggtagtcggg
      181 gttcttggtc gaggtcgagg cccacaggca gcgctgggga cgggcgccct tggactccag
      241 ggcctcccag cgcggcccgc tgaacagctc cagccaggcc tcgtaggcca gcttggcgtt
      301 gtcgatggcg gccgtcccca ggcgggcctc ggctggggcg gcgtcggggc cgccttcctc
      361 gacccgttcc tccagctgcg ggtcgatcag ggtgtcgacc cggctgacga agaacgacgc
      421 cacgctggcc acccggccga ggtccccgct cttggcggcg cggtcctcca ggccggccag
      481 gtaggcctcg accacctccc ggtagcgggc gatcgagaag atcagggtga cgttgacgtt
      541 gaccccggcg gcgatggagg cctggatggc tggcagaccc tcgaccgtgg ccgggatctt
      601 gatcatgacg ttgggcaggc tgagcaggtc gaacagccgg gccgcctgga agatggtctc
      661 gtcggtgtcg tgggcgaggt cgggcggcac ctcgtaggac acatagccgt cgcggccacc
      721 cgtcgcctcg tacaccggtc gcagctcgtg ggcggcccag cggatgtcgt acagggccag
      781 gcgctcgaag atcgcctgcg ccccgagccc ctgctcggcc aggccggcga gctggtcgtc
      841 gtagaagccg ccggcgccga tggcggcctg gaagatggtc gggttggagg tcaccccgac
      901 gaccgagaag tcgtcgcgga gccggcgcag ctcgcccttc tccagccagt cgcgggcgat
      961 cgagtcgacc cagaccgaga cgccctggtc gctgagctgg acgagggggt tggtggtggt
     1021 cattgccgac ctccttggcc tctcaggccg acaggttccg cagcatgcgg gccttctcga
     1081 tatgggtccg gtgcaggacg gccagggccc gctcgaccat gttgtcctcg gtgaacccgt
     1141 acttgatgag ggccgtccca ccaggcgcgg aggccccgaa gcggtcgaca ccgaccaccc
     1201 cgcccccgag accgacccac tgccgccata ggggcgtgct gcccgcctcg accgccagcc
     1261 gagccgtcac ctgcggcggc agcacctcgt cccggtagtc cagcggctgg gcctcgaaca
     1321 gctcccagca gggcatcgac accacccggg tggggatctc ctggcggctc tccagctcgt
     1381 cctgggcggc cagggccagc cagacctccg agccggtggc cagcaggatc agctcgggcg
     1441 cgccgccgcc ggcctcggcc agcacgtacg cgccgcggtg cagtcccgcg gcggaggcat
     1501 accggttccg gtccaccggc ggcagctcct ggcgggacag caccagcgcg gtgggcccgt
     1561 cggtccgttg cagcgcggcc cgccaggcct cggccgtctc cggcccgtcg gccgggcgca
     1621 tcacccagag gttggggatc agccgcaggc tgcccaggtg ctcgaccggc tggtgggtgg
     1681 ggccgtcgcc acccagccca atcgagtcgt gggtccacac atagaccacc ggcagcccca
     1741 tcagggcggc caggcgcacg ctgggccgca tgtagtcgga gaacaccagg aacgtcgcgc
     1801 agaacggccg caggcccccg tgggccgcca gcccgttggc gatcgccccc atgccgtgct
     1861 cgcggatccc gaaccggagg ttgcggccgg cctcggtcgc ggtcaggtcg ccgccgccgg
     1921 cgatgtcggt caggttggac tcggccaggt cggccgagcc gcccaccaag gccggcaggt
     1981 tggccgcgat ggcgttgatg gcctgctggg aggccttgcg ggtggcctgc ttgcgcggct
     2041 cggcgagcag gttgtcgagc tcggcgtccc agccgtccgg cagctccccg gccagggcgg
     2101 cccgcagctc ggcggccagc tccggctcgg cgtcgctgaa ggcacgcagg cgcccctgcc
     2161 aggccgagcg ctcctcccgg ttggtcgcca cccggacctg ccagcgctcc ggcaccccct
     2221 cgggcacctt gaagtgcttg tccgggttcc agccgtagac ctccttggtc gctcggacct
     2281 cgtcctcccc cagggcggcg ccgtgggcct tggaggtgtc ctgggcgttg ggcgcccccc
     2341 aggcgatcac gctgcgcagg cggatgaagc tcggccgctc ggtctcctcc acggcggcct
     2401 ggtaggcggc cgcgatcgcc tccaggtcgt tggcgtcctc cacggccagg gtgtgccagc
     2461 cgtaggcccg gtagcgggcc tccacgtcct cggtgaaggt gatggaggtc ggcccgtcga
     2521 tcgtgatccg gttgtcgtcg tacagcacgc agagcttgcc cagccgcagg tgcccggcca
     2581 gcgacgacgc ctccgacgac accccctcca tcatgtcccc gtcgccgcag aacacccagg
     2641 tccggtggtc caccacctcc tggccgggcc ggttgtagcg ggcggccagc agccgctcgg
     2701 cgatcgcgaa cccgaccccg ttgccgaccc cctgccccag cggcccggtg gtcacctcca
     2761 cccccggcgt gtgcccgtac tcgggatggc cgggggtgag cgacccccac tggcgctgct
     2821 gcttcaggtc ccggatgccc agcgggtagc cggtgaggtg cagcaccgcg tactgcagca
     2881 ccgaagcgtg cccgttggac agcacgaacc ggtcccggtc gaaccattcc gggtcggccg
     2941 ggtcgaactt gaggaaccgg gtccagagca cgtaggcggc cggggccagg gccatggccg
     3001 tccccgggtg gccgctgccc gccttctgga tcgtgtccat ggccagggtc cggacggtgt
     3061 cgaccgccag ccggtcgagg tcggtggcgg gcacggtcgg ggcggtctgg atggtcacgg
     3121 gaactcctcc ttgcttgctt gcccggtcgc ggcgtgggtc aggccatccg gcgcctcatc
     3181 ggccctccag gcgttcaggt cctcgacggc ctcggcggtc ctggcgaacg ccggcagcag
     3241 ccggcggtag aaacgggcgg cgtcccggtc cgggtggacg acctcggtca cgcgcaccag
     3301 ggcggccgcg tcgtcgaccg aggtcagatg tccgagagcg acgtgtccca ggagcgcggc
     3361 cccggcggcc gatccctgct cgctgaccgg gaacccgatc ggcgtcccga ggacgtcggc
     3421 gagcagctgg cgccagaacg ggctgcgggc gaaccctccg gtcgcccgga gctcggccag
     3481 ctccagtccc gccgcctgca tcgcctccag cacgagggcg agctgcaggc acacgccctc
     3541 cagggccgcc cgggccaggt gggctcgggt gtgccgttgg gtcagcccga ggaatgcgcc
     3601 gtacgggtcg cccgcccagc gcggggcgcg ctcccccgac aggtagggca gcatgagcag
     3661 gccctccgcc ccgggtggca cggtggcggc gagctcggtc agcccggcgt cggggtcggg
     3721 caggtcggca ccggccaccc gggccagcca gcggagcacg ttgccgccgt tgttggtcgc
     3781 accgcccacc acccagcggc cggggacgag cacgtagcag aacacccgac cctggtcgtc
     3841 gacgctgggc gcgcggacga cgccacggat ggcggcgctg gtcccgatgg agcaggcagc
     3901 cacacccggg gcgaccgcgt tgacgccgag gttggcgagg acgccgtcgc tggcgccgac
     3961 cacgatcggc gtgccggcct gaagccccca ggcggcgacg tccgcgccga gacgggcgac
     4021 cacgtcggtg gtggcgtgca gggccggcag ctgctcccgg cgcagccccg ccaggtcgag
     4081 cgccgtggtg tcccagtccc gggccgccag cccgtacagc cccgtggccg aggcaatcga
     4141 ctcgtcgacc gccagcagcc cggccagccg ggacagcacg tactccttga tgccgaccca
     4201 gtgggccgcc cggtgccaga cctcgggctg ctgctcccgg taccacagca gcttgaccag
     4261 cggcgacatg gggtggacgg gcgtcccggt gcgccggtgc agcgccagcc actcgccgct
     4321 gtcccgcagc cgccgcgcct gggccgccgc ccggctgtcc gcccaggtca ccgacggcgt
     4381 cagggggctg ccgtcgaggg ccagggcgat caggctgtgc atcgccccgg agaaggcgac
     4441 accggcgatc ggccggccgg ccgcgccagc cctggcggtc acctcgcgga ccacggccgc
     4501 cgaggctgcc gcgaccacgt ccgggtcctg caccgaccag ccggggaagg gcgactcggt
     4561 cgggtactcg cgctcggcgc caccgtggct gaccgcggcg gcgtccacgg ccgcggcctt
     4621 gaccccggtc gtgccgatgt cgacgccgat gatcaggccc ggatctggca ccgttccgcc
     4681 cccgcgatcg ctgctgccac ggcggtcaga cctcctgctc ggtccggtcc tccgaccaca
     4741 gcgtgtggaa gacaccctcg cggtcgaccc gccggtaggt gtgcgccccg aagaagtcac
     4801 gcaggccctg gaccagcgcc gccggcagcc ggtcgcgccg caccgtgtcg tagtaggcca
     4861 gcgccgaagc gaacccgggg acggggaccc cctggctggc cgccagcgag accacccggc
     4921 gccagccgtc ctgggcctgc ccgagcgcct cggcgacatc ggggtcggcc agcaaggtgg
     4981 gcaggtcggc gatggcgtac gcctggcgga tgcgctcgag cagccgggcc cggatgatgc
     5041 agccggcccg ccagatcttg gccaccgtgg ccacgtccac gccccagcgg taggcctcgc
     5101 tggccgcgcc gagctggtcg aggccctggg cgtaggcgac caccttggag gcccacagcg
     5161 cggcccgcac gtcctctacc agctgcttgg ggtcgtcgac cggtcc
//
LOCUS       DASSWR010000475         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_780871,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000475 DASSWR010000000
VERSION     DASSWR010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780871"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VFO47_07975"
     CDS             <1..1461
                     /locus_tag="VFO47_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8788671.1"
                     /translation="VVVGSGAGGGVAAAELAGAGLDVVVLEKGGYAAERDFTHEEGDA
                     YRDLYLYGLTLTTDDLACRILAGSTLGGGTVVNYSTSFPTPPAVLAEWAAVSGVDAFV
                     SGEVAASLEAVAGRLGVTEAQSRPGARDRLLADGLERLGWPAGPLPRNVRGCPQDAGC
                     GWCGFGCRHRAKQSTLVTYLEDAAAAGARLVVGADARRVLVDGGRATGVEAVAGRHRL
                     VVRARAVVAAAGAIETPALLLRSGLGGQVGRHLRLHPGTAAIGSFEEPVRWWEGTLQA
                     RWSAAMRERLGPYAPIFETVPVHPGTAAVALPWQSAAAHRALMERSANLSLVAVLCRD
                     SGAGRVRVDRDGAPRVRWRLGAADEARMAAGLAAAGEVLAAAGAAEVFSLHRRRFAFR
                     PGSPAAHRAWADATRQAGFAGGRATLASYHQMGSCRIGADPATSAVGPDHQTHEVEGL
                     FVADASLFPTASGVNPMLSVMALAHRAAAKVAARLA"
     gene            1607..3127
                     /locus_tag="VFO47_07980"
     CDS             1607..3127
                     /locus_tag="VFO47_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET8788672.1"
                     /translation="MNPRLRAICDLTMSAVREYIGRHEYDGRAEDLSPEGVRAGLSRL
                     GGPPGAAPEPDPHDEAHLAAFERLVRLELGELELHRSNPLYHIAALDVSCYDRDYAPA
                     AERAEARHRHLAAWPDVVDAALAALDRVAAPVAEALLPAARGLAADLEPSGGDPVVER
                     ALDAHKRLTAHLEAAAVEGHRDTALGRGPLERLLGAGEALEVDLGRLEERADAERDRL
                     RALLEEACGRLRPGRPTAEVVAALLEDHPDADGVLREAQALTAEAIAFTLERDLVPGL
                     DGECLVAPAPPSRRWALAMMSWAAPYEADAPSRYYISPPDPGWPASEQDDWLKVFSRT
                     TLPAITVHEVAPGHFAHGRRLRQLTSDVRRTLHSLAFVEGWAHYGEELCIEEGYRGDD
                     PRFAAGVALEALQRVTRLAVSIGTHAGTMTVADGAARFEADAFCYGPAARTEAARATF
                     DPTYGRYTWGKLEILRLRDQARSGWGAGYSHARFHDALLRLGSPPLGLMAAALDRG"
     gene            3291..>4411
                     /locus_tag="VFO47_07985"
     CDS             3291..>4411
                     /locus_tag="VFO47_07985"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HET8788673.1"
                     /translation="MSESASAPDVVERDLHDPYLFINREISWLQFNDRVLAMAADPAV
                     ALLERLKYLAIFSSNLDEFFMVRVAGLRDQVESGMVTRGSDGWTPSETLEAITKHVGP
                     SIEHQIRVFLDEVTPAMAGAGIRIADMSQLDDQALDFLRDYFQRQVFPVLTPLAVDPS
                     HPFPYISNLSLSLAVTVRDPGTARDRFARVKVPGILPRFISLGDGNTFVPLEQVIATH
                     LGKLFPGMDVVEAHPFRVTRDADIELAEDEADDLLIAVEQELRRQRFGVVVRLEVAAT
                     MPDHVVRLLQRELDVEDDALVTVEGPLNLGDLMGLVSSLDRPELADEPWVGTTQPRLL
                     GTEEDSVNLFATLREGDLLVHHPYDAFSTSVQRFIEQAA"
BASE COUNT          513 a         1768 c         1577 g          553 t
ORIGIN      
        1 gtcgtggtcg gctcgggcgc cggcgggggc gtggccgcgg ccgagctggc cggggccggc
       61 ctggacgtgg tcgtgctcga gaagggcggg tatgcggccg agcgcgactt cacccacgag
      121 gagggcgacg cctaccgcga cctgtacctg tacggcctca ccctgaccac cgacgacctg
      181 gcctgccgga tcctggccgg gtcgacgctg ggcgggggca cggtggtcaa ctactcgacc
      241 tcgttcccga ccccgccggc ggtgctggcc gagtgggcgg cggtgtcggg cgtggacgcc
      301 ttcgtctccg gcgaggtcgc cgcctcgctg gaggcggtgg ccggccggct cggggtcacc
      361 gaggcccaga gccgccccgg cgcccgcgac cggctgctgg ccgacggcct ggagcggctc
      421 ggctggcccg ccggcccgct gccccgcaac gtccgcggct gcccccagga cgccggctgc
      481 ggctggtgcg ggttcggctg ccgccaccgc gccaagcagt cgaccctggt cacctacctg
      541 gaggacgccg ccgcggccgg ggcccggctg gtcgtcgggg ccgacgcccg ccgggtcctg
      601 gtcgacggcg gccgggccac cggggtggag gcggtcgccg gccggcaccg gctggtcgtc
      661 cgggcccggg cggtggtggc cgccgccggg gccatcgaga ccccggccct gctgctccgc
      721 tccggccttg gcggccaggt cggccgccac ctgcgcctgc accccggcac cgccgccatc
      781 ggcagcttcg aggagccggt ccgctggtgg gaggggaccc tccaggcgcg ctggtcggcc
      841 gcgatgcgcg agcgcctggg gccgtacgcg cccatcttcg agacggtgcc cgtgcacccg
      901 ggaacggccg cggtcgccct gccctggcag tcggccgccg cccaccgggc cctgatggag
      961 cggtcggcca acctgtcgct ggtcgcggtg ctctgccgcg acagcggcgc cggccgggtc
     1021 cgggtcgacc gcgacggcgc cccccgggtc cgctggcggc tcggcgccgc cgacgaggcc
     1081 cgcatggccg ccggcctggc cgccgccggg gaggtgctgg ccgccgccgg ggcggccgag
     1141 gtgttcagcc tccaccgccg gcggttcgcc ttccgccccg gcagcccggc cgcccaccgc
     1201 gcctgggccg acgcgacccg ccaggccggg ttcgccggcg gccgggccac cctggcctcc
     1261 taccaccaga tgggctcgtg ccgcatcggc gccgacccgg ccacctcggc cgtcggcccc
     1321 gaccaccaga cccacgaggt cgaggggctg ttcgtggccg acgcgtcgct gttcccgacg
     1381 gccagcggcg tcaacccgat gctgtcggtc atggccctgg cccaccgggc cgcggccaag
     1441 gttgcggccc ggctcgcctg agatgccgag gacggaaccg tccggccatg tgccccccaa
     1501 gctcgtgaac cggccccgcg aggtctctca cactcctcga gccacccgaa gatcgcgaag
     1561 tatggaactc gacaatccgg gcgtcggccc cgtaggctcg gcggtcatga accctcgact
     1621 gcgggcgatc tgcgacctga ccatgagcgc ggtccgcgag tacatcggcc gccacgagta
     1681 cgacggccgg gccgaggacc tgtccccgga gggggtccgg gccggcctgt cccggctcgg
     1741 cggccccccc ggcgccgccc ccgagccgga cccgcacgac gaggcccacc tggccgcctt
     1801 cgagcggctg gtccggctgg agctgggcga gctggagctg caccgctcca acccgctgta
     1861 ccacatcgcc gccctggacg tgtcctgcta cgaccgcgac tacgccccgg cggccgagcg
     1921 ggccgaggcc cgccaccgcc acctggccgc ctggcccgac gtggtcgacg ccgccctggc
     1981 cgcccttgac cgggtcgccg ccccggtggc cgaggctctc ctgcccgccg cccgggggct
     2041 ggccgccgac ctggagccgt cggggggcga cccggtggtg gagcgggccc tggacgccca
     2101 caagcgcctg accgcccacc tggaggcggc cgccgtcgag ggccaccgcg acaccgccct
     2161 cggccggggg ccgctggagc gcctgctcgg cgccggcgag gccctggagg tcgacctggg
     2221 ccggctggag gagcgggccg acgccgagcg cgaccggctc cgggccctgc tggaggaggc
     2281 ctgcgggcgg ctgcggccgg gccggcccac cgccgaggtg gtggccgccc tgctcgagga
     2341 ccacccggac gccgacggcg tgctgcggga ggcccaggcc ctcacggccg aggcgatcgc
     2401 cttcaccctg gagcgcgacc tggtgcccgg gctggacggc gagtgcctgg tcgcgcccgc
     2461 acctccgtcg cgccgctggg ccctggccat gatgtcctgg gccgccccgt acgaggccga
     2521 cgccccctcg cgctactaca tctccccgcc cgacccgggc tggcctgcca gcgagcagga
     2581 cgactggctg aaggtgttca gccgcaccac cctgcccgcc atcaccgtgc acgaggtcgc
     2641 ccccggccac ttcgcccacg ggcggcggct gcgccagctc acctccgacg tccgccggac
     2701 cctgcactcg ctggccttcg tcgagggctg ggcccactac ggcgaggagc tctgcatcga
     2761 ggagggctac cgcggcgacg acccccgctt cgccgccggg gtcgccctgg aggcgctcca
     2821 gcgggtcacc cgcctggccg tgtcgatcgg cacccacgcc ggcaccatga cggtcgccga
     2881 cggcgccgcc cgcttcgagg ccgacgcctt ctgctacggc ccggccgccc gcaccgaggc
     2941 cgcccgggcc accttcgacc ccacctacgg ccgctacacc tggggcaagc tggagatcct
     3001 gcgcctccgc gaccaggccc gctccggctg gggcgccggc tacagccacg cccgcttcca
     3061 cgacgccctg ctgcgcctgg gctcgccccc cctcggcctg atggcggccg ccctcgaccg
     3121 gggctgaccc ggcttcgcct tcgccggcgc ctgcggcgcc cccggcctgg cggcgaccat
     3181 cgcctcgacc ctggttccgg gacaggctcc cagcagccca agggctagct tgaacggtcg
     3241 agacatgcaa ggctgatcga cgcagcaccc gaaccaggag ccgaaccgcc gtgagcgaga
     3301 gcgccagcgc cccggacgtg gtcgagcgcg acctgcatga cccctacctg ttcatcaacc
     3361 gggagatctc ctggctccag ttcaacgacc gggtcctggc gatggccgcc gacccggcgg
     3421 tggcgctcct ggaacggctg aagtacctgg ccatcttctc ctccaacctg gacgagttct
     3481 tcatggtccg ggtggccggc ctgcgcgacc aggtcgagtc gggcatggtc acccgcgggt
     3541 cggacggctg gacgccgagc gagaccctgg aggcgatcac caagcacgtc ggcccgagca
     3601 tcgagcacca gatccgggtg ttcctggacg aggtcacccc ggccatggcc ggggccggca
     3661 tccgcatcgc cgacatgtcc cagctggacg accaggccct ggacttcctg cgcgactact
     3721 tccagcgcca ggtcttcccg gtgctgacgc ccctggccgt cgaccccagc cacccgttcc
     3781 cctacatctc caacctgtcg ctgtcgctgg ccgtgaccgt ccgcgacccg ggcaccgccc
     3841 gcgaccgctt cgccagggtc aaggtgccgg gcatcctgcc ccggttcatc tccctgggcg
     3901 acggcaacac cttcgtgccc ctggagcagg tgatcgccac ccacctgggc aagctgttcc
     3961 cgggcatgga cgtggtcgag gcccacccgt tccgggtgac ccgcgacgcc gacatcgagc
     4021 tggccgagga cgaggccgac gacctgctga tcgccgtcga gcaggagctg cgccgccagc
     4081 gcttcggggt ggtggtccgg ctcgaggtgg ccgcgaccat gcccgaccac gtggtccggc
     4141 tcctccagcg cgagctcgac gtcgaggacg acgccctggt caccgtggag gggccgctca
     4201 acctgggcga cctgatgggg ctggtgtcgt ccctcgaccg gcccgagctg gccgacgagc
     4261 cgtgggtcgg gaccacccag ccgcggctgc tcggcaccga ggaggactcg gtcaacctgt
     4321 tcgccaccct gcgcgagggc gacctgctgg tccaccaccc ctacgacgcc ttctcgacct
     4381 cggtgcagcg cttcatcgag caggccgccg a
//
LOCUS       DASSWR010000476         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1089929, whole genome shotgun sequence.
ACCESSION   DASSWR010000476 DASSWR010000000
VERSION     DASSWR010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089929"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VFO47_07990"
     CDS             <1..1166
                     /locus_tag="VFO47_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8788674.1"
                     /translation="LVIAGGPGWTEAAAAGLRAVAEASQLPVAVSFRSQDVLDNRSPS
                     YVGDLGVSPNPALAERVRAADLVVAVGPRLGEITTGAYRLLEAPVPRQRLVHVHPGLA
                     ELGRVYQPDLAVNAAVGPFVAAWRSVPPVRGEAWAVWTEAARANYLAWVRPWPAQGGA
                     LGKAPGSISGKAPGSAPGKAPDSASGTVPTSDPAKTPGTVDLGQVMAALRERLPDDAV
                     ITSGAGNFTGWVHRFFQFRRLGTQVAPKSGAMGFGLPAALAAKVVHPGRPVVAVTGDG
                     DFLMTGQELATAVQHRLDVVVLVANNGMYGTIRMHQERTYPGRVIATDLRNPGFAALA
                     EAYGAHGETVTDAEEFPAALERALTAGRPAVLDLHVDPDAIAPGLSLTALRRH"
     gene            1213..2028
                     /locus_tag="VFO47_07995"
     CDS             1213..2028
                     /locus_tag="VFO47_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HET8788675.1"
                     /translation="MGRLDDLDLSLRLSRQEEAERLETASNRLLELRLTLGGLLGDGR
                     LGPPVCVLFEGWDASGKGGAIRRLVHPMDPRHVRVATFAAPTYDEKRHHFLLRFGSSL
                     PGWGGMAVLDRSWYGRVLVERVEGFATVDQWSRGYDEIVDFERSLALEGMILLKFWMH
                     ISDEEQLRRFKRRETEPLKTWKLTDEDWRNREKRAAYEQAIEDMLERTDHPLAPWFLI
                     PAESKRYARVNVVETAIAEIERGMRERGFEPPTPRPRPLGKTRTKGKSGKAQT"
     gene            2438..>2875
                     /locus_tag="VFO47_08000"
     CDS             2438..>2875
                     /locus_tag="VFO47_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET8788676.1"
                     /translation="MFRNLPIRTKLIICVLGPLLMLAVLALVGIRQNQAESDRAARST
                     AFARLAAGLAPLVHQLQGERSLSISYIDSGRRQWEEALRRQRRAVDQAAATYQAETQR
                     LSAGDELLAEKIEYGLSELDRLGEQRNTIDGAKIDPSKLAVGPG"
BASE COUNT          395 a         1081 c         1031 g          368 t
ORIGIN      
        1 cgctggtgat cgccggcggc cccggatgga ccgaggcggc cgcggccggc ctgcgagccg
       61 tggccgaggc cagccagctg ccggtggcgg tctcgttccg ctcccaggac gtgctcgaca
      121 accgctcccc cagctacgtc ggtgacctcg gggtgagccc caacccggcg ctggccgagc
      181 gggtcagggc ggccgacctg gtggtggccg tcgggccccg cctcggggag atcaccaccg
      241 gcgcctaccg gctgctggag gcgccggtcc cccgccaacg gctcgtccac gtccaccccg
      301 gcctggccga gctgggccgg gtctaccagc ccgacctggc cgtcaacgcc gccgtgggcc
      361 cgttcgtggc cgcctggcgc tcggtcccgc cggtgcgggg cgaggcctgg gcggtctgga
      421 cggaggcggc ccgggccaac tacctggcct gggtgcggcc gtggccggcc cagggcggcg
      481 ccctcggcaa ggccccgggc agcatttccg gcaaggcccc gggcagcgct cccggcaagg
      541 cccccgacag cgcctccggc acggtaccca ccagcgaccc cgccaagacc cccgggacgg
      601 tcgacctcgg gcaggtgatg gcggccctgc gcgagcggct gcccgacgac gcggtgatca
      661 ccagcggggc cgggaacttc accggctggg tccaccgctt cttccagttc cggcggctcg
      721 ggacgcaggt ggcgcccaag agcggcgcca tgggcttcgg cctcccggcc gcgctggccg
      781 ccaaggtcgt ccacccgggc cgcccagtgg tggcggtgac cggcgacggc gacttcctga
      841 tgaccggcca ggagctggcc acggccgtcc agcaccgcct ggacgtggtc gtgctggtgg
      901 ccaacaacgg catgtacggc accatccgca tgcaccagga gcggacctac ccgggccggg
      961 tgatcgcgac cgacctgcgg aaccccggct tcgccgccct ggccgaggcc tacggcgccc
     1021 atggcgagac ggtcaccgac gccgaggagt tcccggccgc cctggagcgg gcgctgaccg
     1081 ccggacgccc ggccgtgctc gacctgcacg tggatcccga cgcgatcgcc cccggtctct
     1141 cgctgaccgc cctgcggcgc cactgagcta gtcctcttgc cggttgcggg cggctgctgc
     1201 caggatcagc ggatgggacg cctcgacgac ctcgacctga gcctcaggct gagccgccag
     1261 gaggaggcgg agcggctgga gacggcctcc aaccggctgc tggagctgcg gctcaccctg
     1321 ggcgggctgc tgggggacgg gcggctgggg ccgccggtgt gcgtgctgtt cgagggttgg
     1381 gacgcctccg gcaagggcgg cgccatccgc cgcctggtcc atccgatgga cccccggcac
     1441 gtgcgggtgg ccaccttcgc cgccccgacc tacgacgaga agcggcacca cttcctgctc
     1501 cggttcgggt cgagcctgcc cgggtggggc ggcatggccg tgctcgaccg ctcctggtac
     1561 gggcgggtcc tggtcgagcg ggtggagggg ttcgccacgg tcgaccagtg gagccggggc
     1621 tacgacgaga tcgtcgactt cgagcgctcg ctggcgctcg agggcatgat cctgctcaag
     1681 ttctggatgc acatctcgga cgaggagcag ctgcgccggt tcaagcggcg cgagaccgag
     1741 ccgctcaaga cctggaagct gactgacgag gactggcgca accgcgagaa gcgcgccgcc
     1801 tacgagcagg ccatcgagga catgctggag cggaccgatc atccgctggc cccctggttc
     1861 ctgatcccgg ccgagtccaa gcgctacgcc agggtcaacg tcgtggagac ggccatcgcc
     1921 gagatcgagc ggggcatgcg cgagcgcggc ttcgagccac cgaccccccg cccgcggccc
     1981 ctcggcaaga ccaggaccaa gggaaagagc ggcaaggccc agacctagcg cgcttgcgcc
     2041 ggtccgacca ggacggcggc gaccggctcg gcctgctgat cgtggcttcc tggcccacgg
     2101 ccacgggttc gcggtccggc tcggcccctc ccccagggcg gcgtcaccgc caccgcgcgc
     2161 cttcccgcga cggtcctctc ggcccgcgcc ccggaccccg tccggccggg ctgacaccgc
     2221 cggcgccttg accggcgtcg tgccgccgct cggccgcggg aaggagtgat acgtagttcg
     2281 agcgacccat tagggactac gccgccgcgc accccttgag ctacgacaca tcggtctgac
     2341 cagccgagag tccgcagatt cccgcattgt ggcgggcttg acagaaacgg tagcgtcaag
     2401 cgccttcctc tcccgccgta cgcaaggttg gtcgcagatg ttccgtaacc tgccgatccg
     2461 caccaagctc atcatctgcg tgctcggtcc cctgctgatg ctggccgtcc tcgccctcgt
     2521 gggcatccgc cagaaccagg ctgagagcga ccgggccgcc cgcagcaccg ccttcgcccg
     2581 cctggccgcc ggcctggcgc ccctggtcca ccagctccag ggcgagcgca gcctctcgat
     2641 cagctacatc gacagcggcc gccggcagtg ggaggaggcg ctgcgcaggc agcggcgcgc
     2701 ggtcgaccag gcggccgcca cctaccaggc cgaaacccag cggctcagcg ccggcgacga
     2761 gctgctggcc gagaagatcg agtacggcct gagcgagctg gaccgcctcg gcgagcagcg
     2821 gaacacgatc gacggggcca agatcgaccc gtccaagctg gcggtcgggc ccggc
//
LOCUS       DASSWR010000477         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1373852, whole genome shotgun sequence.
ACCESSION   DASSWR010000477 DASSWR010000000
VERSION     DASSWR010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373852"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFO47_08005"
     CDS             <1..1108
                     /locus_tag="VFO47_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8788677.1"
                     /translation="ADRLLEYLEDFGLPDLEALGAEILERSERATREAIAALPDGDYT
                     HTIRTDGLDEPVTIACRVQVRGDEIVVDYAGSSAQVDRGMNVVPNYTAAYTSYALKCA
                     ISPEVPHNDGSFRPVSTVAPEGSILNPRFPAAVAARHIVGHFLPHAVFGALVKVIPDR
                     VLAEGAGNIWLTTVRGQGRDRFITVFFAAGGTGARPTKDGLSTTSFPSGIATAPVEVI
                     ETTSPLVIRSKELRRDSGGPGRFRGGLGQRIEVEVRTGEPFVVSSLADRMRFPADGYQ
                     GGGPGAPGGFRTSLGTRPNPKLSLALPAGTRFVLELPGGGGFFDPLTRQPEAVAGDVA
                     EGLVSPAAAERDYGVRVTRAGRLLGLGERRPGDA"
     gene            complement(1105..2112)
                     /locus_tag="VFO47_08010"
     CDS             complement(1105..2112)
                     /locus_tag="VFO47_08010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8788678.1"
                     /translation="MERVGFAVGYDPAMDGRDMAHWMGEAERRGYEIGFFSETIELMR
                     DSVSSLTAIGLATERLTIGCTQITRLRTPLVMAQTAAALDELTGGRLLLAPGACTSTH
                     AKRHGLEPADPGVALREWVESMRAILTGERVSYSGQVVHFDDVQLGFKPARDRIPMFI
                     PATSRKGLRIAGQIGDGVMLNAVCSPEYSANALKIVREAAEEAGRDWESFAVYQLVNC
                     SVEDDEQTALDAIRWEVASKLDPIQLPFIAGPKMRVGEPYFHPEDFPKFEEAWNRGGK
                     EALIAAVPDSYIRGMTASGTPDQVKAKVQQFRDVGVQVPILRPAAAHQAERLLDLFAP
                     G"
     gene            2235..>2808
                     /locus_tag="VFO47_08015"
     CDS             2235..>2808
                     /locus_tag="VFO47_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8788679.1"
                     /translation="MSSDQGVETGPSQDHVDHILEQWAQQRPDLDVSPMGIIGRMSRL
                     SRFLERSIATVLARYGLNEPQFAVLAALRRAGPPHCLSPTDLYNGLLVSSGAMTNRLE
                     RLAAAGLVERVPDPGDGRSLLVALTDKGQQVVNEAVAAHTANEHRLLSSLSLEERTRL
                     ADVLRRLLLQFEDHPPPSRSRDPGQAATGRS"
BASE COUNT          374 a         1045 c         1002 g          387 t
ORIGIN      
        1 cgccgaccgg ctgctcgagt acctggagga cttcggcctg cccgacctgg aggcgctcgg
       61 ggccgagatc ctggaacgct ccgagcgggc gaccagggag gccatcgccg ccctgcccga
      121 cggcgactac acccacacca tccgcaccga cgggctggac gagccggtca ccatcgcctg
      181 ccgggtccag gtgcgcggcg acgagatcgt ggtcgactac gccggcagct cggcccaggt
      241 cgaccggggc atgaacgtgg tccccaacta caccgccgcc tacaccagct acgccctcaa
      301 gtgcgccatc agcccggagg tgccgcacaa cgacggcagc ttccgccccg tctccaccgt
      361 cgcccccgag ggcagcatcc tcaacccccg cttcccggcc gccgtggccg cccgccacat
      421 cgtcggccac ttcctgcccc acgccgtgtt cggcgccctg gtcaaggtca tccccgaccg
      481 cgtgctggcc gagggcgccg gcaacatctg gctgaccacg gtgcgcggcc agggccgcga
      541 ccgcttcatc acggtgttct tcgccgccgg cggcaccggc gcccggccga ccaaggacgg
      601 gctgtcgacg accagtttcc ccagcggcat cgccaccgcc ccggtcgaag tcatcgagac
      661 cacctccccc ctggtgatcc gctccaagga gctgcgccgc gactccggcg ggcccggccg
      721 cttccggggc gggctgggcc agcgcatcga ggtggaggtg cgcaccggcg agccgttcgt
      781 cgtctcctcc ctggccgacc ggatgcgctt tcccgccgac ggctaccagg gaggcgggcc
      841 gggggcgccg ggcgggttcc ggacctcgct cggcacccgc cccaacccca agctgagcct
      901 ggccctcccg gccggcaccc ggttcgtgct cgagctgccg ggcgggggcg ggttcttcga
      961 cccgctcacc cgtcagcccg aggccgtggc cggcgacgtg gccgaggggc tggtcagccc
     1021 ggccgcggcc gagcgtgact acggggttcg ggtgacccgg gcgggccggc tcctcgggct
     1081 cggcgagcgc cgtcccggcg acgcctagcc gggcgcgaac aggtcgagca ggcgctcggc
     1141 ctggtgggcg gcggccggcc gcaggatcgg gacctggacc ccgacgtcgc ggaactgctg
     1201 caccttggcc ttgacctggt cgggggtgcc gctggcggtc atgccgcgga tgtaggagtc
     1261 gggcacggcc gcgatcaggg cctccttgcc gccccggttc caggcctcct cgaacttggg
     1321 gaagtcctcg gggtggaagt agggctcgcc gacccgcatc ttcggcccgg cgatgaacgg
     1381 cagctggatg gggtcgagct tgctggccac ctcccagcgg atggcgtcca gggcggtctg
     1441 ctcgtcgtcc tccaccgagc agttgacgag ctggtagacg gcgaaggatt cccagtcgcg
     1501 gccggcctcc tcggccgcct cgcgcacgat cttgagggcg ttggccgagt actcgggcga
     1561 gcagacggcg ttcagcatca ccccgtcgcc gatctggccg gcgatgcgca gccccttgcg
     1621 gctggtggcg gggatgaaca tcgggatccg gtcgcgggcc ggcttgaagc cgagctggac
     1681 gtcgtcgaag tggaccacct ggccggaata gctgacccgc tcgccggtga ggatggcgcg
     1741 catggactcg acccactcgc gcagcgccac cccggggtcg gccggctcca ggccgtggcg
     1801 cttggcgtgg gtgctggtgc aggcgccggg ggccagcagc agccggccgc cggtcagctc
     1861 gtccagggcg gcggccgtct gggccatgac caggggggtg cgcagccggg tgatctgggt
     1921 gcagccgatg gtcagccgct cggtggccag cccgatggcg gtgagcgagc tgaccgagtc
     1981 gcgcatcagc tcgatcgtct cggagaagaa gccgatctcg tagccgcgcc gctcggcctc
     2041 gcccatccag tgggccatgt cgcggccgtc catggcgggg tcgtagccga ctgcgaaccc
     2101 gacgcgctcc atctccgacc cccatcatcc cggctgccca gcaccaaagt atcttagcgt
     2161 ttagatacct taccgccctg gtcgcgtcaa ggcgcggaga ggacggggcg gggcgacggc
     2221 cgtagaatct cctcatgtcg agcgaccagg gcgtcgagac gggaccgtcg caggaccacg
     2281 tcgaccacat cctcgagcag tgggcccagc agcggccgga cctggacgtg tccccgatgg
     2341 gcatcatcgg ccgcatgtcg cggctgtcgc ggttcctgga gcgcagcatc gccaccgtcc
     2401 tggcccgcta cggcctgaac gagccgcagt tcgcggtgct ggcggcgctg cgccgggccg
     2461 gtccgcccca ctgcctgtcc cccaccgacc tgtacaacgg gctgctggtg tcgtcggggg
     2521 ccatgaccaa ccggctggaa cggctggccg cggccggcct ggtcgagcgc gtgcccgacc
     2581 cgggggacgg ccgcagcctg ctggtggccc tcaccgacaa gggccagcag gtggtcaacg
     2641 aggcggtggc cgcccacacc gcgaacgagc accggctgct gtcctcactg agcctggagg
     2701 agcgcacacg gctcgccgac gtcctgcgcc ggctgctgct gcagttcgag gaccatccgc
     2761 cgccgagccg gtcccgcgac cccggccagg ccgctactgg gcgctcca
//
LOCUS       DASSWR010000478         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_215966,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000478 DASSWR010000000
VERSION     DASSWR010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215966"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3078
                     /locus_tag="VFO47_08020"
     CDS             <1..3078
                     /locus_tag="VFO47_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788680.1"
                     /translation="VGVGAAGAIYLRAAGESLLAQNLRTATSHTAGLHVSQVVADGKE
                     LRNLTESVAAAGPAVPALAPPVAGLETRTPVPIRAEGGQPANAYLAGRDGLCDHLVLQ
                     AGRCPGARPLGLPAEAALSRRAADWLRLDVGDRFRVEGFGAGAAGRDLMVAGIYTPTP
                     RDEFWFGAQQAYFPPPPLDNEQPPMDAVFLANADLLPVVDGGTMDIRARFDHFIQPDR
                     LRLADTRAVPAALGEVAEVLRVTNPNATVLTGLVDLVAKAEADRQALTVPVLLADVQL
                     AALALLILVVVSAMTAEARAGEVALAKVRGATTGQAFGLAVLELAVIAALALPAGLAV
                     GWLGAYLLARSQLEAGIPVAVTPAAVVVALAAAAVALGAASLASLGAVRRRILDQWRR
                     GRSDQVGRRAVVLDVVLLLVAAAALANLRASGTRQATGGYDLLATLAPGLAVLAAALV
                     LGRSLPLVAGWLVRFTGRSAGVATWVGARQVARRGGPALRVVIALAAAFGLVAFAVTV
                     RQDMARNRHDRAFTLVGAAERLQVVLPSDTLGPERVAEADPSGAAAMAVARYPAILRD
                     APAVTAALVGVEPDRYPSVGFRRDDFSGRSLEQTMAALAAAPVSPLDLGQADQLEVAV
                     GAGEIRADGPVRLAALVQGARRSARVDLGRLEAGDVRPLRGRLDPAGGPWRLQRLWVQ
                     REPGVISAVGVTLTFDSVRAGQGGAWRLVEGFDDPARWYSLNDNAYEPGDTLSATTGV
                     DGAALRVAVAAPDSGATIGIGHASVPEALPAVVTPAFMAAVSAKVGRVVRVRGPMPGD
                     LLLQVTGVASVLPGAVGSSAAAFVDADWLLVHAQRNPNDRPAAGEVWTVGGERGRAVA
                     ERIAAGGARVESRTAAADLEADLAQQAPSLALLLLLVGAAAGAALATGGVMLHLYLTG
                     RRRAFELAVLEVFGARRGDLWGPVAVEQGWLVGYGVLCGGLVGLLVALIALPAIPQFT
                     DRPTVPPPIYTPDWAVLGLALGLTVLLVGAGLAAVVAALVHQARPALLREEEL"
     gene            3075..>3535
                     /locus_tag="VFO47_08025"
     CDS             3075..>3535
                     /locus_tag="VFO47_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788681.1"
                     /translation="MSLTAHKAKGVADGRRARAGERLLAGDGLFQLYVLDGREVVALQ
                     GVDFGVDWGEMVALLGPSGSGKSTMLAILGGLERPSAGRVLLDGVDVTRLSEAQLTVL
                     RRRRIGFVWQGAARNLLPHASAWENLDLPMVLAGLPAGRRRRRARVLQDAV"
BASE COUNT          318 a         1405 c         1363 g          449 t
ORIGIN      
        1 gtcggggtgg gggcggccgg cgccatctac ctgcgcgccg ccggggagtc gctgctcgcc
       61 cagaacctgc gcaccgccac cagccacacc gccggcctcc acgtcagcca ggtcgtggcc
      121 gacggcaagg agctgcgcaa cctcaccgag tcggtggccg cggccgggcc ggccgtgccc
      181 gccctggccc cgccggtcgc cggcctggag acccgcaccc cggtcccgat ccgggccgag
      241 ggcggccagc cggccaacgc gtacctggcc ggccgcgacg gcctctgcga ccacctggtg
      301 ctccaggctg gccgctgccc cggagccagg ccgctcggcc tgccggccga ggccgccctg
      361 agccgccggg ccgccgactg gctccggctg gacgtcggcg accggttccg ggtcgagggg
      421 ttcggggccg gcgccgccgg gcgcgacctg atggtggccg ggatctacac ccccaccccc
      481 cgcgacgagt tctggttcgg cgcccagcag gcctacttcc cgcccccgcc gctcgacaac
      541 gagcagccgc ccatggacgc cgtgttcctg gccaacgccg acctgctgcc ggtggtcgac
      601 ggcgggacca tggacatccg ggcccgcttc gaccacttca tccagccgga ccggctgcgc
      661 ctggccgaca cccgggccgt ccccgccgcc ctcggcgagg tggccgaggt gctgcgggtc
      721 accaacccca acgccaccgt cctcaccggg ctggtcgacc tggtcgccaa ggccgaggcc
      781 gaccgtcagg ccctcaccgt gccggtgctc ctggccgacg tccagctggc cgccctggcc
      841 ctgctgatcc tggtcgtggt ctccgccatg accgccgagg ccagggccgg cgaggtcgcc
      901 ctggccaagg tgcggggcgc caccaccggg caggccttcg ggctggccgt cctggagctg
      961 gccgtcatcg ccgccctcgc cctcccggcc gggctggcgg tcggctggct gggcgcctac
     1021 ctgctggccc gcagccagct cgaggccggc atcccggtgg ccgtcacccc ggcggcggtg
     1081 gtggtggcac tggccgccgc cgccgtcgcc ctgggcgcgg ccagcctggc cagcctgggc
     1141 gcggtccggc ggcggatcct cgaccagtgg cgccgcggcc gctccgacca ggtcggccgg
     1201 cgggcggtcg tcctggacgt ggtcctgctg ctggtcgccg cggccgccct ggccaacctg
     1261 cgcgcctccg ggacccgcca ggccaccggc ggctacgacc tgctcgccac cctcgccccc
     1321 ggcctggccg tgctggccgc cgccctggtc ctcgggcgct cgctgcccct ggtcgcgggc
     1381 tggctggtcc gcttcaccgg ccgctcggcc ggggtggcca cctgggtcgg ggcccgccag
     1441 gtggcccgcc gcggcggtcc cgccctgcgg gtggtgatcg ccctggccgc cgccttcggg
     1501 ctggtcgcgt tcgcggtcac cgtccgccag gacatggccc gcaaccgcca cgaccgggcc
     1561 ttcaccctgg tcggggcggc cgagcggctc caggtggtgc tgcccagcga caccctcggc
     1621 cccgagcggg tggccgaggc cgacccgtcg ggggcggcgg ccatggccgt ggcccgctac
     1681 ccggccatcc tgcgcgacgc cccggcggtc accgccgccc tcgtcggggt cgagcccgac
     1741 cgctacccgt cggtggggtt ccggcgggac gacttctccg ggcgctcgct tgagcagacc
     1801 atggccgcgc tggccgccgc gccggtgtcc ccgctggacc tcggccaggc cgaccagctc
     1861 gaggtggccg tcggcgccgg cgagatccgg gccgacgggc cggtccggct ggccgccctg
     1921 gtgcaggggg cgcgccgctc cgcccgggtc gacctgggcc gcctggaggc cggggacgta
     1981 cgcccgctgc ggggccggct cgacccggcc gggggaccgt ggcggctgca gcggctctgg
     2041 gtccagcgcg agccgggcgt gatcagcgcg gtcggcgtca ccctcacctt cgactcggtc
     2101 cgggccggcc agggcggcgc ctggcggctg gtggagggct tcgacgaccc ggcgcgctgg
     2161 tactcgctca acgacaacgc ctacgagccg ggcgacaccc tcagcgccac caccggggtc
     2221 gacggcgccg ccctccgcgt cgccgtcgcc gcccccgact cgggggccac catcggcatc
     2281 ggccacgcct cggtgcccga ggccctgccg gccgtggtca ccccggcgtt catggccgcc
     2341 gtgtcggcca aggtgggccg ggtggtgcgg gtccgcggcc cgatgccggg cgacctgctg
     2401 ctccaggtga cgggggtggc cagcgtgctc ccgggcgccg tcgggtcgag cgccgccgcc
     2461 ttcgtcgacg ccgactggct gctggtccac gcccagcgca accccaacga ccggccggcc
     2521 gccggcgagg tctggaccgt cggcggcgag cgcggccggg ccgtggccga gcggatcgcc
     2581 gccggcgggg ccagggtcga gtcgcgcacc gccgccgccg acctggaggc cgacctggcc
     2641 cagcaggcgc cgtcgctggc cctgctgctc ctgctcgtcg gggcggccgc cggggccgcc
     2701 ctggccaccg gcggggtcat gctccacctg tacctgaccg gccggcggcg ggccttcgag
     2761 ctggccgtgc tcgaggtgtt cggggccagg cggggcgacc tgtgggggcc ggtggcggtc
     2821 gagcaggggt ggctggtcgg ctacggggtg ctctgcggcg gcctggtcgg cctgctcgtc
     2881 gccctgatcg ccctcccggc catcccccag ttcaccgacc ggccgaccgt gcccccgccg
     2941 atctacaccc ccgactgggc cgtcctcggg ctcgcccttg ggctgaccgt gctgctggtc
     3001 ggggccggcc tggccgccgt ggtcgccgcc ctcgtccacc aggcccgccc ggccctgctg
     3061 cgtgaggagg agctgtgagc ctcacggcgc acaaggcgaa gggggtggcc gacgggcggc
     3121 gggccagggc cggggagcgg ctgctggccg gcgacgggct gttccagctg tacgtgctgg
     3181 acgggcgcga ggtggtcgcc ctccaggggg tcgacttcgg ggtcgactgg ggcgagatgg
     3241 tcgcgctgct cgggccgtcg ggttcgggca agtcgaccat gctggcgatc ctcggcggcc
     3301 tggagcggcc gtcggcgggg cgggtcctgc tggacggcgt cgacgtcacg cggctgtccg
     3361 aggcccagct caccgtgctg cggcggcggc ggatcgggtt cgtctggcag ggggcggccc
     3421 gcaacctgct cccgcacgcc tccgcctggg agaacctcga cctgccgatg gtcctggccg
     3481 ggctgccggc gggccggcgc cggcgccggg ccagggtgct gcaggacgcg gtcga
//
LOCUS       DASSWR010000479         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1007404, whole genome shotgun sequence.
ACCESSION   DASSWR010000479 DASSWR010000000
VERSION     DASSWR010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007404"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFO47_08030"
     CDS             complement(<1..285)
                     /locus_tag="VFO47_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788682.1"
                     /translation="MSTLVLLREQIAANRRAYQVTVAVVVAALALFGLLVTLVLGLGV
                     SGFGAGLGLAVLVVGFAPGVGERAALRDAGAQPADPVRWPRYHNLVEGLSQ"
     gene            complement(282..734)
                     /locus_tag="VFO47_08035"
     CDS             complement(282..734)
                     /locus_tag="VFO47_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788683.1"
                     /translation="MDVAAALWGGIIGAIVAATVYAGFLGLRLTRLDLLRFEGGLLLR
                     ERSSMVYIYGVTVQVVAGALLALVYRQVFQQLDSATFVGWGALLGLAQGVLAVLLLPV
                     LGRLNRNVRAGDEPPPGPLGRAYGRLTPLALLVASVVFGTWVGIVLLP"
     gene            complement(781..1890)
                     /locus_tag="VFO47_08040"
     CDS             complement(781..1890)
                     /locus_tag="VFO47_08040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8788684.1"
                     /translation="MNIGLVCPYEWTVPGGVGNHVRALAGELRQAGHRVDVLAPAERP
                     VAEPGFVGLGGSLAVPYNGSVARIAIGPRTLVRVRRAVHRGGYDLLHVHEPLSPSVGM
                     LTVAQAGPVPVVGTFHANLGRSLALEAASPLLRRVYDRLAARIAVSASARDTWQGHFG
                     GAMAVVPNGVASEFFARPKPLEGWKGDGPTVLFVGRLEPRKGLEYLVRAFLRLKPAFP
                     RLRLLVVGRDDRHIQDKAMAMVPPRLRPDLVFVGSVPQADLPSYYASADVFCAPSLGG
                     ESFGIVLAEAMAVGLPVVCSDIGGYREVVRDGADGLLVPPRDPEALAAALAGLLDNPA
                     RLAAMGEAAAVSARRYAWEVVAAEVAEVYQAALAG"
     gene            complement(1887..>2711)
                     /locus_tag="VFO47_08045"
     CDS             complement(1887..>2711)
                     /locus_tag="VFO47_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HET8788685.1"
                     /translation="PVAFVLGWAGGLLYLRVDRDRREALTANLAQVLGPGTRPRQLRR
                     AVRRGFVSYGRYWVEAFRLEDLSPADIRERLRIEGREHIDTALAGGRGAIFASPHIGN
                     WDAGGAWLAASGYPATAVVERLRPAELYERFAVYRRALGLELLPLEDGAETLRGVVRA
                     LRAGRLACLVCDRDLTGGGLEVRLFGARAVMPGGPASIALRTGAPLLPCSVYHDRRPG
                     RWRAVVHPPLVPERSGDTRKDTLALTQRLAEEFEGLIAAAPEQWHVLSRYFRGSAA"
BASE COUNT          392 a         1026 c         1038 g          255 t
ORIGIN      
        1 ctggctcagg ccctcgacca ggttgtggta gcggggccag cggaccgggt cggccggctg
       61 ggcgccggcg tcgcgcaggg cggcccgctc ccccaccccc ggagcgaacc cgaccaccag
      121 cacggccagg cccagcccgg ccccgaaccc gctcaccccg aggcccagga cgagggtgac
      181 caggagcccg aacagggcca gggcggccac gaccacggcc accgtgacct ggtaggcgcg
      241 gcggttggcg gcgatctgct cccgcaggag caccagcgtg ctcaaggaag gaggacgatg
      301 ccgacccagg tgccgaagac caccgaggcg accagcaggg ccaggggggt gaggcggccg
      361 taggcccgcc ccaggggacc gggcggcggc tcgtcgccgg cccgcacgtt gcggttgagc
      421 ctgcccagga ccggcagcag gagcacggcc aggacgccct gggcgaggcc gagcagggcc
      481 ccccagccga cgaaggtggc cgagtcgagc tgctggaaca cctggcggta gacgagggcg
      541 agcagggcgc cggcgaccac ctgcacggtc accccgtaga tgtagaccat gctgctccgc
      601 tcccggagca gcaggccgcc ctcgaagcgg agcaggtcca gccgggtcag gcgcagcccc
      661 aggaacccgg cgtacaccgt cgccgccacg atcgccccga tgatcccgcc ccacagggcc
      721 gccgccacgt ccacgccgcc tcctcgactc gccgctcgac ggcgcagtgt accgaaacgc
      781 tcatcccgcg agagcggcct ggtagacctc ggccacctcg gcggcgacca cctcccaggc
      841 gtagcggcgg gcggacaccg cggccgcctc gcccatggcg gccaggcggg ccgggttgtc
      901 gagcagaccg gccagggccg cggccagggc ctcggggtcg cggggcggga ccagcagccc
      961 gtcggcgccg tcgcggacca cctcgcggta gccgccgatg tccgagcaga ccaccggcag
     1021 gccgacggcc atggcctcgg ccaggacgat cccgaacgac tccccgccca gcgacggggc
     1081 gcagaacacg tcggccgagg cgtagtagga gggcaggtcg gcctggggca cggagccgac
     1141 gaagaccagg tcgggccgga gccggggcgg gaccatggcc atggccttgt cctggatgtg
     1201 ccggtcgtcg cggccgacca ccagcaggcg cagccgcggg aacgccggct tgagccgcag
     1261 gaacgccctg accaggtact ccaggccctt gcggggctcg agccggccga cgaacaggac
     1321 cgtcgggccg tcgcccttcc agccctcgag gggcttgggg cgggcgaaga actcggacgc
     1381 gaccccgttg gggacgaccg ccatggcgcc gccgaagtgg ccctgccagg tgtcccgggc
     1441 cgaggcgctg acggcgatcc tggccgccag ccggtcgtag acccggcgca gcagggggct
     1501 ggccgcctcc agggccaggg agcggccgag gttggcgtgg aaggtcccga ccaccgggac
     1561 cgggcccgcc tgggccacgg tcagcatgcc tacgctcggg gacagcggct cgtgcacgtg
     1621 gagcaggtcg tagccgcccc ggtggacggc ccgccggacc cggaccaggg tccgggggcc
     1681 gatggcgatg cgggccaccg agccgttgta ggggacggcc agcgacccgc cgaggccgac
     1741 gaagccgggc tcggccaccg gccgctcggc cggggcgagc acgtcgaccc ggtggccggc
     1801 ctggcgcagc tcgccggcca gggcccggac gtggttgccg accccgccgg gcacggtcca
     1861 ctcgtacggg cagaccaggc cgatgttcat gccgccgagc cgcggaagta gcggctgagc
     1921 acatgccact gctcgggcgc ggccgcgatc aggccctcga actcctccgc cagccgctgg
     1981 gtcagggcca gggtgtcctt gcgggtgtcg ccggagcgct cggggacgag cggcgggtgg
     2041 acgacggccc gccagcgccc gggccggcgg tcgtggtaga cgctgcatgg cagcagcggc
     2101 gccccggtcc ggagggcgat cgaggccggc ccgcccggca tcaccgcgcg ggcgccgaac
     2161 agccgcacct ccaggccgcc cccggtcagg tcgcggtcgc agaccaggca ggccagccgg
     2221 ccggcccgca gggccctgac caccccgcgc agggtctcgg cgccgtcttc gagcggcagc
     2281 agctccaggc caagggcccg gcggtagacg gcgaagcgct cgtacagctc ggccgggcgc
     2341 agccgctcca ccaccgccgt ggccgggtag ccggaggcgg ccagccaggc cccgccggcg
     2401 tcccagttgc cgatgtgggg gctggcgaag atcgctcccc ggccgccggc cagggcggtg
     2461 tcgatgtgct cgcggccctc gatccgcagc cgctcgcgga tgtcggccgg gctcaggtcc
     2521 tcgagccgga acgcctccac ccagtagcgg ccgtagctga cgaagccgcg ccgcaccgcc
     2581 cggcggagct ggcggggcct ggtcccgggg ccgaggacct gggccaggtt ggccgtcagg
     2641 gcctcacggc ggtcacggtc cactcggaga tacagcagcc ccccggccca ccccaggacg
     2701 aacgccaccg g
//
LOCUS       DASSWR010000480         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_498349,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000480 DASSWR010000000
VERSION     DASSWR010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498349"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFO47_08050"
     CDS             complement(<1..590)
                     /locus_tag="VFO47_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788686.1"
                     /translation="MALGGLAACTAGVPQTGDVVTVSPVTTSVPQEDADALKDLGDPS
                     SGLSEVEVATGYMRAMNTGDVTRIQRWVVSRDHDKVKRWSENVTTVRVYSVFEPGQPQ
                     LREDGRRVVPVKVKLVGQLQDGRDWYPITGDDVLSLELGNDGGDARVANPRGLWMRDV
                     NFARLYASTEVYLVADKTRPAPRLAAAPVFVRRGDG"
     gene            complement(634..>2038)
                     /locus_tag="VFO47_08055"
     CDS             complement(634..>2038)
                     /locus_tag="VFO47_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET8788687.1"
                     /translation="VQQVKLLTRPGEISEVSGVNTEQIQEALKDQLIKRLGGQPNFEA
                     VVLRSEDGTPSSSDEDFRIEHVPAGLVERVEGGDELAYVYIRSPVGPGRMLVVGAPIR
                     LDQSDAGIQQAYFFYPLNFQEETLAKLSNFLLIVGAGLLAAVVILAAISIGRVLKPIQ
                     RARDVAEEIAAGNLAARIPQATSQDDFGKLAESFNRMTDNLAQKIGELEHVGSLQARF
                     VSDVSHELRTPLATVRMAADYIHAARAGLPPDAQRAAVLLERELERFENLLEDLLEIS
                     RFDAGVINLEPVEVDLSGLLDEVVDALDPIAHGRKVDVALEVDRSQGQPLVAADPRRL
                     DRVFSNLVKNAIEHTVEGGVRIWVGRRARDVVVTVADEGEGIPAEDLPHIFERFYRAD
                     VHRARTLGGTGLGLAIALENVNLHRGSIDVQSEVGQGSIFTVTLPAIDPKPEPADGRA
                     AEPQPEPAPVPEVAVGG"
BASE COUNT          261 a          713 c          756 g          308 t
ORIGIN      
        1 tcgccgtcgc ccctgcggac gaagaccggc gcggccgcca gccgcggcgc cgggcgggtc
       61 ttgtcggcga ccaggtagac ctcggtcgag gcgtacagcc tggcgaagtt gacgtcccgc
      121 atccagaggc cgcgggggtt ggccacccgg gcgtcgccgc cgtcgttgcc cagctccagg
      181 ctgagcacgt cgtccccggt gatcgggtac cagtcgcgac cgtcctggag ctggccgacc
      241 agcttgacct tgaccgggac gacccgccgc ccgtcctcgc ggagctgcgg ctggccgggc
      301 tcgaagacgc tgtagacccg cacggtggtg acgttctcgg accagcgttt gaccttgtcg
      361 tggtccctgc tcaccaccca ccgctggatc ctcgtgacgt cgccggtgtt catcgcccgc
      421 atgtagccgg tcgccacctc gacctcgctc agtccggagg aggggtcgcc gaggtccttg
      481 agggcgtcgg cgtcctcctg cggcaccgag gtcgtgaccg ggctgaccgt caccacgtcg
      541 ccggtctggg gcacgccggc cgtgcaggcc gccagcccac ccagcgccac cagggcggcg
      601 atcggacgga ggaggcggtg gcgcgggggg cggctatcca ccgacggcga cctccgggac
      661 gggcgccggc tcgggctgcg gctcggccgc ccggccgtcc gccggctcgg gcttggggtc
      721 gatggccggc agggtgacgg tgaagatcga gccctggccg acctcgctct gcacgtcgat
      781 cgagccacgg tgcaggttca cgttctcgag ggcgatggcc agcccgagcc cggtgccgcc
      841 gagggtcctg gcccggtgga cgtcggcccg gtagaagcgc tcgaagatgt ggggcaggtc
      901 ctcggccggg atgccctcgc cctcgtcggc cacggtgacg accacgtcgc gggcgcgccg
      961 gccgacccag atgcggaccc cgccctcgac ggtgtgctcg atggcgttct tgaccaggtt
     1021 ggagaagacg cggtcgagcc ggcgcgggtc ggccgcgacc agcggctggc cctggctgcg
     1081 gtccacctcg agggccacgt cgaccttgcg cccgtgggcg atcgggtcga gggcgtcgac
     1141 cacctcgtcg agcagcccgc tgaggtcgac ctcgaccggc tccaggttga tcaccccggc
     1201 gtcgaagcgg ctgatctcca ggaggtcctc gagcaggttc tcgaaccgct ccagctcgcg
     1261 ttccagcagc acggcggccc gctgggcgtc gggagggagg ccggcgcggg cggcgtggat
     1321 gtagtcggcg gccatgcgca ccgtggccag gggcgtgcgc agctcgtggc tgacgtcgga
     1381 gacgaaccgg gcctgcaggc tgccgacgtg ctccagctcg ccgatcttct gggccaggtt
     1441 gtcggtcatc cggttgaacg actcggccag cttgccgaag tcgtcctggc tggtcgcctg
     1501 ggggatccgg gcggccaggt tgccggccgc gatctcctcg gcgacgtccc tggcccgctg
     1561 gatcggcttg agcacccggc cgatcgagat ggcggccagg atgaccacgg ccgcgagcag
     1621 cccggcaccc acgatcagca ggaagttgga gagcttggcg agcgtctcct cctggaagtt
     1681 cagggggtag aagaagtagg cctgctggat gccggcgtcg ctctggtcga ggcggatcgg
     1741 ggcccccacc accagcatgc ggcccgggcc gacgggcgag cggatgtaca cgtaggccag
     1801 ctcgtcgccg ccctcgaccc gctcgaccag gccggcgggg acgtgctcga tcctgaagtc
     1861 ctcgtcggag gagctcgggg tcccgtcctc gctccgcagg accacggcct cgaagttcgg
     1921 ctggccgccc agccgcttga tcagctgatc cttgagcgcc tcctggatct gctcggtgtt
     1981 gacgccggag acctcactga tctcgccggg ccgggtgagg agcttgacct gctggacg
//
LOCUS       DASSWR010000481         6271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_894365,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000481 DASSWR010000000
VERSION     DASSWR010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894365"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFO47_08060"
     CDS             <1..945
                     /locus_tag="VFO47_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788688.1"
                     /translation="MPVIRRQHKIDYLGATTIVGAVTCLLLYLDWRGSLYGWTETGAM
                     ALLGGAAALAALFLWIERRAVEPIIPMRLFRIQVFSVGNAFAFLAGIAMFGTIIFLPV
                     YFQAVMGMSPTQSGLALLPAIFGIFSTSITSGQLISRTGRYKIYPVIGAVIMTIALLT
                     LSRLGVDTPFWQVAIYTYLFGAGLGFTMQTIVTAVQNSVERRDMGAATSSITFFRQMG
                     GSVGAALFGAVLSSRLAHYLAEQLAAAGIRPGAGGPRVEANNVQAIQQLTEPVRSVVL
                     HAFTNALDDVFLVGVPFVAVAIVVALLLKEEPLRTGQP"
     gene            967..1860
                     /gene="thrB"
                     /locus_tag="VFO47_08065"
     CDS             967..1860
                     /gene="thrB"
                     /locus_tag="VFO47_08065"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET8788689.1"
                     /translation="MERPIHVRAPATSANLGPAFDAAGIALDWWDELEIRPAPVDGIQ
                     LSGEQAELLPTGRDNLIRVAMRRLAEAAGTPLPPLHLSVVKGFPLGRGFGSSAAAVVL
                     GLVAARRLVAPDLPEAELLALAYELEGHPDNATPCLHGGATLAWVEDGRPRRRALPVH
                     PDLVAVALVAPEPMATSEARRLLPERVPFAVAAHTGARAAMLPLALAGDHELLLPATE
                     DLLHQPERLAHTPDGAKLLERLRSAGHAAFVSGAGPSLLVLCPRPAAAGVVADAEEAL
                     AADGAEGWSVRPLELARHGAL"
     gene            1975..4197
                     /locus_tag="VFO47_08070"
     CDS             1975..4197
                     /locus_tag="VFO47_08070"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET8788690.1"
                     /translation="MPDKLTSPAGEEFVLADASVNRVLVKGVVAVMHARTERLWSTKE
                     GDMFLCADAEGNLDAEQATGAGFYWRDTRYLSDFRLEVDGHAPLLLSTSADRAFASHV
                     DLTNQDLLEDDGSVAAVQGTVNIRRTRVVDGRLYERIRIKSYNAAAVTLTVRLTFGTD
                     FADIFEVRGLKRAVRGRLARPKADRRSAVFAYEGEDSVFRETRIAFELDPTRLDVVDD
                     QVVAEWELRLEPTQTELIALTVEPRAATAAAGPEHSFDVVMHDLRRSYETWERACTRV
                     WTDNELYNSLLSRGLRDLRALLTPTRHGSLVAAGIPWFVAPFGRDSLLTCHQTLMVNP
                     DLTRNTLEVLAAFQADEVDEWRDAQPGKILHELRQGELAGAGIIPHTPYYGSIDSTPL
                     WLLLLGTYYRWTGDLEFCRGLLPSVERALAWIADYGDPDGDGFVEYQRSSPRGLANQG
                     WKDSHDSVGHVDGKLAEGPIALAEVQAYIYLAKLRLAEVFEAMGDAQRAATLRAEADG
                     LRRAFNEAFWVESEQFYAMALDGDKRQVTAISSNPAHGLYCGIVDPERAGPMARRLLA
                     PDMFSGWGVRTLSKSAAAYNPMSYHNGSIWPHDNAIIGAGLKRYGFAKATNRLATAMF
                     EMAVTVDDMRLPELFCGFTRRSPNRPVAYPVACSPQAWAAGAPFLLLQAMLGISADAP
                     ANTINVNRPHLPGWLNTVELHALRVGGSTVSIVFQRRGETTGFSLLDKEGDVRVLMEE
                     "
     gene            4582..>6271
                     /gene="rho"
                     /locus_tag="VFO47_08075"
     CDS             4582..>6271
                     /gene="rho"
                     /locus_tag="VFO47_08075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET8788691.1"
                     /translation="MATTERDILEGKVLPELQAIASSMGVQGYQRLRKADLIGAIIAK
                     AQGVEFVPSSTPARGRRGGTTTEAPEAQAEATPAVPDQPELPGAGEAEASSNGPERRT
                     RRRSRGPADAEAPPEGSGEPHNGPAGSASVEMFGNGGQPAAAGAPPATLTEPSSNGEV
                     THEGPAGSGSGPAPSGGAEEAPVTQAEQPAPAAEAGSPPATRTEGPQGDQAQGDQAQG
                     DKPQGDQQAQAGDGQQQRGDGQQDQGGGGDRGQGQRRDRDRDRDWGGQGGGNRWENQQ
                     GQGGGRRRNRNRNRNRNRGGGGGGFNDQGNVALLERPDYRQQDDRPEVSRTGTLDIRS
                     QDGYGFLRVNGYLPGSEDVYVPLSMIRRMGLRRGDEIEGTVKLPRDSEKYAALSRVDK
                     VNGLEPEEARNRRDFKDLTPLYPLERLRLEHNPTEISTRIMDLMCPIGKGQRGLIVSP
                     PKAGKTTLMKQIANAISDNNPEVYLIILLTDERPEEVTDMQRSTKAHVIYSTFDRPSE
                     EHCQVAELTIERAKRLVEYGRDVVVLLDGITRLSRAYNLATPASGKILSGGVDSS"
BASE COUNT          898 a         2386 c         2172 g          815 t
ORIGIN      
        1 atgccggtga tccgccgcca gcacaagatc gactacctgg gcgccaccac catcgtcggc
       61 gccgtcacct gcctgctgct gtacctggac tggcggggca gcctttacgg ctggaccgag
      121 accggggcga tggccctgct cggcggggcc gccgccctgg ccgccctgtt cctctggatc
      181 gagcgccggg ccgtcgagcc gatcatcccc atgcggctgt tccgcatcca ggtgttcagc
      241 gtcggcaacg ccttcgcctt cctggccggc atcgccatgt tcgggaccat catcttcctg
      301 cccgtctact tccaggcggt catgggcatg tccccgaccc agtcgggcct ggccctgctg
      361 ccggcgatct tcgggatctt ctcgacctcg atcacctccg ggcagctgat ctccaggacc
      421 ggccgctaca agatctaccc ggtgatcggg gccgtgatca tgaccatcgc cctgctcacc
      481 ctgtcgcggc tgggcgtgga cacgccgttc tggcaggtgg cgatctacac ctatctgttc
      541 ggggccggcc tcggcttcac catgcagacg atcgtcaccg ccgtgcagaa ctcggtcgag
      601 cgccgggaca tgggcgcggc gaccagctcg atcaccttct tccggcagat gggcgggtcg
      661 gtcggggcgg ccctgttcgg ggccgtgctg tccagccggc tggcccacta cctggccgag
      721 cagctcgccg cggcgggcat ccggccggga gccggcgggc cccgggtgga ggccaacaac
      781 gtccaggcca tccagcagct caccgagccg gtcaggagcg tggtcctgca cgccttcacc
      841 aacgccctgg acgacgtgtt cctcgtcggc gtcccgttcg tggccgtggc catcgtggtc
      901 gcgctgctgc tgaaggagga gccgctgcgc accggccagc cgtgacctcg cggtaggctg
      961 ccgggcatgg aacgacccat ccacgtccgc gccccggcga ccagcgcgaa cctggggccg
     1021 gcgttcgacg cggccgggat cgccctcgac tggtgggacg agctcgagat ccgcccggcc
     1081 ccggtcgacg gcatccagct cagcggcgag caggccgagc tgctccccac cgggcgcgac
     1141 aacctgatca gggtggccat gcgccgcctg gccgaggcgg ccgggacgcc cctgccgccg
     1201 ctgcacctct cggtcgtcaa gggcttcccg ctcggccgcg ggttcgggtc gagcgccgcc
     1261 gccgtcgtcc tcggcctggt cgcggcccgc cggctggtcg ccccggatct ccccgaggcc
     1321 gagctgctgg ccctggccta cgagctggag ggccaccccg acaacgccac cccgtgcctg
     1381 cacggcgggg ccacccttgc ctgggtggag gacggccgcc cccgccggcg ggccctgccc
     1441 gtccatcccg acctggtcgc cgtggccctg gtcgcccccg agccgatggc gaccagcgag
     1501 gcccgccggc tgctgcccga gcgggtgccg ttcgcggtcg cggcccacac cggcgcccgc
     1561 gcggccatgc tgcccctggc cctggccggc gaccacgagc tgctcctgcc ggccaccgag
     1621 gacctcctcc accagcccga gcggctggcc cacaccccgg acggggccaa gctgctggag
     1681 cggctgcggt cggccggcca cgccgccttc gtctccgggg ccgggccctc cctgctggtc
     1741 ctctgccccc gtccggcggc cgccggggtg gtggccgacg ccgaggaggc cctggccgcc
     1801 gacggggccg agggctggtc ggtccggccg ctggagctgg cccgccacgg cgccctctga
     1861 ggcgccctcg cggggtggac gccggcggta cactgcgggc tcaagtcgcc tggcggtccg
     1921 ccgaaggatg aggcagccgc tttctgtgcc gcagccaatg acgcggagtc tcccgtgccc
     1981 gacaagctga cctcgcccgc cggcgaggag ttcgtgctgg ccgacgcctc ggtcaaccgc
     2041 gtgctggtca agggcgtggt ggccgtcatg cacgcccgca ccgagcgcct gtggtcgacc
     2101 aaggaaggcg acatgttcct ctgcgccgac gccgagggga acctggacgc cgagcaggcc
     2161 accggggccg gcttctactg gcgcgacacc cgctacctgt ccgacttccg gctggaggtc
     2221 gacggccacg cacccctgct gctgtcgacc tcggccgacc gggccttcgc cagccacgtc
     2281 gacctgacca accaggacct gctggaggac gacggctcgg tcgcggccgt ccagggcacg
     2341 gtcaacatcc gccgcacccg ggtcgtcgac ggccgcctgt acgagcgcat ccggatcaag
     2401 agctacaacg cggccgcggt caccctgacc gtccgcctga ccttcggcac cgacttcgcc
     2461 gacatcttcg aggtccgcgg gctcaagcgg gccgtcaggg gccggctggc ccggcccaag
     2521 gccgaccggc gctcggcggt gttcgcctac gagggcgagg acagcgtctt ccgcgagacc
     2581 cgcatcgcct tcgagctcga cccgaccagg ctcgacgtcg tcgacgacca ggtggtcgcc
     2641 gagtgggagc tgcggctgga gccgacccag accgagctga tcgcgctcac cgtcgagccg
     2701 cgggcggcca cggcggccgc cggccccgag cactccttcg acgtcgtcat gcacgacctg
     2761 cgccgctcct acgagacctg ggagcgggcc tgcacccggg tctggaccga caacgagctg
     2821 tacaactcgc tgctgtcgcg gggcctgcgg gacctccggg cgctgctcac cccgacccgg
     2881 cacggcagcc tggtggcggc cgggatcccc tggttcgtgg ccccgttcgg ccgcgactcg
     2941 ctgctcacct gccaccagac cctgatggtc aacccggacc tgacccggaa caccctggag
     3001 gtgctggccg ccttccaggc cgacgaggtc gacgagtggc gcgacgccca gccgggcaag
     3061 atcctgcacg agctgcgcca gggggagctg gccggggccg ggatcatccc ccacaccccc
     3121 tactacggct cgatcgactc gaccccgctg tggctgctgc tgctcggcac ctactaccgc
     3181 tggaccgggg acctggagtt ctgccggggg ctgctgccca gcgtggagcg ggccctggcc
     3241 tggatcgccg actacggcga ccccgacggc gacggcttcg tcgagtacca gcgctccagc
     3301 ccccgcggcc tggccaacca gggctggaag gactcgcacg attcggtggg gcacgtcgac
     3361 ggcaagctcg ccgagggccc gatcgccctg gccgaggtcc aggcctacat ctacctggcc
     3421 aagctgcgcc tggccgaggt gttcgaggcc atgggcgacg cccagcgggc ggcgaccctg
     3481 cgggcggagg cggacggcct gcgccgcgcc ttcaacgagg ccttctgggt cgagtcggag
     3541 cagttctacg cgatggccct cgacggcgac aagcgccagg tgacggccat ctcctccaac
     3601 ccggcgcacg ggctgtactg cggcatcgtc gaccccgagc gggccgggcc catggccagg
     3661 cggctgctgg cgccggacat gttctccggc tggggggtgc ggaccctgtc caagtcggcc
     3721 gccgcctaca acccgatgag ctaccacaac ggctcgatct ggccgcacga caacgccatc
     3781 atcggcgccg ggctgaagcg ctacgggttc gccaaggcga ccaaccggct ggccacggcc
     3841 atgttcgaga tggccgtgac ggtcgacgac atgcgcctgc ccgagctgtt ctgcggcttc
     3901 acccggcgct cgcccaaccg gccggtggcc tacccggtcg cctgctcgcc ccaggcgtgg
     3961 gccgcgggcg ccccgttcct gctgctccag gccatgctcg ggatctcggc ggacgccccg
     4021 gccaacacca tcaacgtgaa ccggccgcac ctgcccggct ggctcaacac cgtcgagctg
     4081 catgctctca gagtgggcgg ctccacggtg tcgatcgtgt tccagcgccg gggggagacg
     4141 accgggttct cgctgctgga caaggagggc gacgtccgcg tgctgatgga ggagtgacgc
     4201 ctgttgcgct cggtcggcca aggcccgtac aatgcgaaac gtctcagtcg ctggccaatt
     4261 cagtcgacgc ccccgcctta tcggctcatt cttcggcgat ttcggcgatc ccggcgaggc
     4321 tggccagcgg tggcaaccac cagttccgtt cgagcagtcg accgggtcac gtgatcggac
     4381 gccagccctc ggcggtgtgc ggttcgtgcc gcgccgcccg gccccgtcga cgggaacagg
     4441 attaggagcg agcgcgagtt tctccccacg gtgccgcagc tgacacggca cccccagatc
     4501 tcaagacccc ggcaaccggg cggcgaccac ggtccccggc tcctcccgcc ggcacgtcgc
     4561 cgcctcacag ggaaggtgaa catggcaact accgaacgag acatcctcga gggaaaggtc
     4621 ctgccagagc tgcaggccat cgcctcctcc atgggtgtcc agggctacca gcggctccgc
     4681 aaggcggacc tgatcggcgc gatcatcgcc aaggcccagg gtgtcgagtt cgtgccctcg
     4741 tcgaccccgg cccggggccg gcggggtgga acgaccaccg aggcgccgga ggcccaggcc
     4801 gaggccacgc ccgcggtgcc cgaccagccc gagctgccgg gcgccggtga ggccgaggcc
     4861 tccagcaacg gccccgagcg ccggacccgc cgccgttccc gcggcccggc cgacgccgag
     4921 gcgccgccgg aggggtccgg ggaaccccac aacggcccag ccggcagcgc atcggtggag
     4981 atgttcggga acggtggcca gccggccgcc gccggcgccc cgccagcgac cctgaccgag
     5041 cccagcagca acggcgaggt cactcacgaa ggcccagccg gcagcgggtc cggtccggca
     5101 ccgtccggcg gggccgagga agccccggta acccaggccg agcagccggc cccggccgcc
     5161 gaggcgggct cgcccccggc gacccggacc gagggccccc agggcgacca ggcccagggc
     5221 gaccaggccc agggcgacaa gccgcagggc gaccagcagg cccaggccgg cgacggccag
     5281 cagcagcgcg gcgacgggca gcaggaccag ggcggcggcg gcgaccgcgg ccagggccag
     5341 cggcgcgacc gcgaccggga ccgtgactgg ggcggccagg gcggcggcaa ccgctgggag
     5401 aaccagcagg gccagggcgg cggccggcgc cgcaaccgca accgcaaccg gaaccgcaac
     5461 cgcggtggcg ggggcggcgg cttcaacgac cagggcaacg tcgccctgct ggagcggccc
     5521 gactaccgcc agcaggacga ccggcccgag gtcagccgca ccggcaccct cgacatccgc
     5581 tcccaggacg gctacggctt cctgcgggtc aacggctacc tgccgggctc cgaggacgtc
     5641 tacgtgcccc tgtcgatgat ccgccggatg ggcctgcgcc gcggcgacga gatcgagggg
     5701 acggtcaagc tgccccgcga ctccgagaag tacgccgccc tgtcccgggt cgacaaggtc
     5761 aacggccttg agcccgagga ggcgcgcaac cgccgcgact tcaaggacct gaccccgctg
     5821 tacccgctgg agcggctgcg cctggagcac aacccgaccg agatctcgac gcggatcatg
     5881 gacctgatgt gccccatcgg gaagggccag cgcgggctga tcgtctcccc gcccaaggcg
     5941 ggcaagacca cgctgatgaa gcagatcgcc aacgccatca gcgacaacaa ccccgaggtg
     6001 tacctcatca tcctgctcac cgacgagcgg cccgaagagg tcaccgacat gcagcgctcg
     6061 accaaggcgc acgtcatcta ctcgaccttc gaccgcccca gcgaggagca ctgccaggtc
     6121 gccgagctca ccatcgagcg ggccaagcgg ttagtggaat atggccggga cgtggtcgtg
     6181 ctgctggacg gcatcacccg gctctcgcgg gcctacaacc tggccactcc ggccagcggc
     6241 aagatcctgt ccggtggtgt cgactcctcg g
//
LOCUS       DASSWR010000482         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1204168, whole genome shotgun sequence.
ACCESSION   DASSWR010000482 DASSWR010000000
VERSION     DASSWR010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204168"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFO47_08080"
     CDS             <1..534
                     /locus_tag="VFO47_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788692.1"
                     /translation="AWVPEERSELLWRVDRATNRVMGRVRLRKVLYGPVGVASVGDTV
                     WVTCCALEHGARPAGMLLRVDGRRNQVTARVPVPEGPMAVVAGRDRIWVGTARGSLLS
                     VDAASARVVTRVPPPDPRGRIQALSAAPGALWVADTGAGAVRRFDLASGRYDLGVTAP
                     AAQAIPPRRAPTPSTTR"
     gene            complement(486..2429)
                     /locus_tag="VFO47_08085"
     CDS             complement(486..2429)
                     /locus_tag="VFO47_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET8788693.1"
                     /translation="MPGVRHGRLLALALALTGAVLAGCSGGEAPRGTVNVPMYDNFFA
                     REVTRVPAGSTVRFPNEGRAPHNAVAVDGTWRTPERIGADEAATVVLDQPGVYRFYCT
                     FHATEDGRQGMAATMVVGDVTFYPGQEDGGPAVAPVKRASGVTRRVPEDHDTIQAAVD
                     AAKPGDLVLVGPGTYREEVKVAVPSLVIRGRDRDEVVVDGEFQRPNGIAVTADGVAVE
                     NLTVRNALINGLFWTGVRGYRASYVTAYNNKDYGIYAFDSVDGLFEHSYASGSPDSGF
                     YIGQCHPCDAVIDGVLAENNGLGYSGTNAGGNLQIVRSTWRRNWAGIVPNTLDSELLP
                     PFRQVDIVGNLVEANHNRGAPGVPLEWGPFGNGIVLGGGNDSRVERNRVRDHVHHGVL
                     VTPNLDEHIWTSSGNRVVGNVVEGSGRADLALAAPAGDGNCFQGNQARTSIPVGLQAF
                     QRCSGLRLPLRMDLSTTLASLGQVAEANTGAYPMNRPEDQPAPPPQPQLPGGAAAPVR
                     PAVDVFAAARVDLDRVPVPGAPDLPPTVRKEPTVFGVPILAASPWQLVFALYGYLLPF
                     VLYAAWTSLALWDLARRDDLSRGAAIGWIAVILLVPFLGVIAYHVLGRPRLPAWLRAA
                     VIGGGLFAYLVVLGVGALLGGIA"
     gene            2474..3709
                     /locus_tag="VFO47_08090"
     CDS             2474..3709
                     /locus_tag="VFO47_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET8788694.1"
                     /translation="MGSGFRFAVGIEDTFVPHAFPGHRALDEYELTQHYRFWSEDLGL
                     AAAAGAGAIRYGFPWYRLNPAPGRFAWDWADRVVDRLDTLGLEAIVDLVHYGTPLWLD
                     NAFLHTDFPDRLAEYAAALADRYRDRLTLWTPMNEPQITAMHCGEQGIWPPRLTGHDG
                     YVKLVRAIVRGVVAAQRAVVEASGGRASFVHVEATFRYAGATEAFAEEVELLRARQFL
                     IQDLLCGMVDDDHPLAGYLRRHGFGDDDLAWAARNPAHPDVLGVNYYPHLTTAEYRAG
                     EVVPAALRPRRDDWTAGLEELLRAFAARYRRPLMVTETSVLGGVARRLAWLDASLELV
                     AGLRDQGLEIVGYTWWPLFDLVGWEYRDALGPVGEHLVRMGLYDLAADEIGTLHRRAT
                     PVADRFRSWTTSNRFTRGS"
     gene            3764..>4282
                     /locus_tag="VFO47_08095"
     CDS             3764..>4282
                     /locus_tag="VFO47_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET8788695.1"
                     /translation="MLAQERRDAILRALMPSGAATVAELAGALAVSEMTIRRDLGALA
                     AQQLVEKVHGGAVLAKGGAAEPHFAAKRRVNAPAKAAIAAAAVALVHDGMTVALSAGT
                     TTWQVARRLRGGGVRDLTFVTNSLNIAGALEANGWHSIVVSGGSFRTPSDALVGPFAN
                     HVLRQLNTDLLFL"
BASE COUNT          542 a         1556 c         1608 g          576 t
ORIGIN      
        1 gcctgggtcc cggaggagcg gtcggagctg ctgtggcgcg tcgaccgggc caccaaccgg
       61 gtgatgggac gggtccggct gcgcaaggtg ctgtacgggc cggtcggggt ggcctcggtg
      121 ggcgacaccg tctgggtgac ctgctgcgcg ctcgagcacg gcgcacgccc ggccgggatg
      181 ctgctgcggg tggacggccg ccgcaaccag gtcaccgccc gcgtcccggt cccggaaggc
      241 ccgatggcgg tggtcgccgg ccgggacagg atctgggtgg ggaccgcccg tgggtcgctg
      301 ctgtccgtcg acgccgcctc ggccagggtg gtgacgcggg tgccgccgcc ggatcccagg
      361 ggccggatcc aggcgctgtc ggccgcgccg ggagccctgt gggtcgccga cacgggcgcg
      421 ggcgcggtcc ggcggttcga tctggcgtcc ggccgctacg atctgggagt caccgcgccg
      481 gccgctcagg cgatcccgcc caggagggcg ccgaccccga gcacgaccag gtaggcgaac
      541 aggccgccgc cgatcacggc cgcccgcagc caggcgggga ggcgggggcg gcccaggacg
      601 tggtaggcga tcacgcccag gaacggcacc agcaggatga ccgcgatcca gccgatggcc
      661 gcgccgcggc tcaggtcgtc gcggcgggcc aggtcccaca gggccaggga ggtccaggcg
      721 gcatacagca cgaacggcag caggtagccg tagagggcga agacgagctg ccagggggag
      781 gcggcgagga tcgggacgcc gaacacggtt ggctccttgc ggacggtcgg tggcaggtcg
      841 ggggcgccgg gaaccgggac ccggtccagg tcgaccctgg cggcggcgaa gacgtcgacg
      901 gccgggcgaa ccggcgccgc ggccccgccg gggagctgcg gctggggcgg gggcgccggc
      961 tggtcctcgg gccggttcat cgggtaggcg ccggtgttgg cctcggcgac ctggccgagg
     1021 ctggccaggg tggtggacag gtccatgcgc agcggcagcc gtagccccga gcaccgctgg
     1081 aaggcctgca gccccaccgg gatcgaggtc cgggcctggt tgccctggaa gcagttgccg
     1141 tcgccggccg gggcggccag ggccaggtcg gcccggcccg acccctcgac cacgttgccc
     1201 accacccggt tgcccgacga ggtccagatg tgctcgtcca ggttgggggt gaccaggacg
     1261 ccgtggtgga cgtggtcgcg gacccggttg cgctcgaccc gcgagtcgtt cccgccaccg
     1321 aggacgatgc cgttgccgaa cgggccccac tccagcggca ccccgggcgc gccgcggttg
     1381 tggttggcct cgaccaggtt gccgacgatg tcgacctgcc ggaacggcgg cagcagctcc
     1441 gagtcgaggg tgttggggac gatcccggcc cagttgcgcc gccaggtgga gcggacgatc
     1501 tggaggttgc cgccggcgtt ggtgcccgag tagccgaggc cgttgttctc ggccagcacc
     1561 ccgtcgatga cggcgtcgca gggatggcac tggccgatgt agaagcccga gtcgggcgag
     1621 cccgaggcgt aggagtgctc gaacaggccg tcgaccgagt cgaaggcgta gatgccgtag
     1681 tccttgttgt tgtaggcggt cacgtagctg gcccggtagc cgcgcacccc ggtccagaac
     1741 agcccgttga tgagcgcgtt gcggacggtc aggttctcga ccgcgacccc gtcggcggtc
     1801 acggcgatcc cgttgggccg ctggaactcg ccgtccacca ccacctcgtc gcggtcgcgg
     1861 ccgcggatca ccagcgaggg cacggccacc ttgacctcct cgcggtaggt cccggggccg
     1921 accaggacca ggtcgcccgg cttggccgcg tcgacggcgg cctggatggt gtcgtggtcc
     1981 tcgggcaccc gccgggtgac cccgctggcc cgcttcaccg gcgccaccgc cgggcccccg
     2041 tcctcctggc ccgggtagaa ggtgacgtcg ccaacgacca tggtggcggc catgccctgg
     2101 cggccgtcct cggtggcgtg gaaggtgcag tagaagcggt acacgcccgg ctggtcgagc
     2161 acgaccgtgg ccgcctcgtc ggccccgatc cgctccggcg tccgccaggt cccgtcgacg
     2221 gccacggcgt tgtgcggcgc ccggccctca ttggggaacc ggacggtgga cccggccggc
     2281 acccgggtca cctcgcgggc gaagaagttg tcgtacatgg gcacgttgac cgtgccccgc
     2341 ggcgcctccc cgcccgagca cccggccagc accgccccgg tcagcgccag cgccagcgcg
     2401 agcaggcggc catgccgaac gccgggcacg acgcctcctt cacccgcctg taggtcgcgg
     2461 gtatcttgcc acgatgggca gcggcttccg gttcgcggtc gggatcgagg acaccttcgt
     2521 cccccatgcc ttccccgggc accgggcgct ggacgagtat gagctgaccc agcactaccg
     2581 cttctggtcc gaggacctgg gcctggccgc cgcggccggc gccggggcga tccgctacgg
     2641 gttcccctgg taccggctga acccggcgcc ggggcggttc gcctgggact gggccgaccg
     2701 ggtggtggac cggctggaca ccctcggcct ggaggccatc gtcgacctgg tccactacgg
     2761 caccccgctg tggctggaca acgccttcct gcacaccgac ttcccggacc gcctggccga
     2821 gtacgcggcc gccctggccg accgctaccg ggaccggctc accctctgga cgccgatgaa
     2881 cgagccccag atcaccgcca tgcactgcgg cgagcagggc atctggccgc cccggctgac
     2941 cggccacgac ggctacgtca agctggtccg ggcgatcgtg cggggggtgg tcgcggccca
     3001 gcgggcggtc gtcgaggcca gcggcggccg ggccagcttc gtccacgtcg aggccacctt
     3061 ccggtacgcg ggggcgaccg aggccttcgc cgaggaggtc gagctgctcc gggcccgcca
     3121 gttcctcatc caggacctgc tctgcggcat ggtcgacgac gatcacccgc tggccggcta
     3181 cctgcgccgc cacggcttcg gcgacgacga cctggcctgg gccgcgcgca acccggccca
     3241 ccccgacgtc ctcggcgtca actactaccc gcacctgacc accgccgagt accgggccgg
     3301 ggaggtggtc ccggccgccc tccgcccccg gcgcgacgac tggacggccg ggctggagga
     3361 gctgctccgc gccttcgccg cccgctaccg gcgtcccctg atggtcaccg agaccagcgt
     3421 cctgggcggg gtcgcccggc ggctggcctg gctggacgcc tccctggagc tggtggccgg
     3481 cctgcgagac cagggcctgg agatcgtcgg ctacacctgg tggccgttgt tcgacctggt
     3541 tggctgggag taccgggacg ccctcgggcc ggtgggggag cacctggtca ggatgggcct
     3601 gtatgacctg gcggcggatg agatcggcac cttgcaccgc cgcgccaccc cggtggccga
     3661 ccggttccgg tcctggacga cctcgaacag gttcacacgc ggttcctgat ggagtctgtt
     3721 cggtgtcggt cggatacggt acggtagggc cgaggaggcc gatgtgcttg cccaggaacg
     3781 tcgcgacgcc atcctgcggg ccctgatgcc ctcgggtgcc gccaccgtcg ctgagctggc
     3841 cggcgcactg gccgtctcgg agatgaccat ccggcgcgac ctgggcgcgc tcgccgccca
     3901 gcagctcgtc gagaaggtcc acggcggggc cgtgctggcc aagggcggcg ccgccgagcc
     3961 ccacttcgcc gccaagcgcc gcgtcaacgc cccggccaag gcggccatcg ccgcggcggc
     4021 cgtcgccctc gtccacgacg gcatgacggt ggccctgtcc gccgggacca cgacctggca
     4081 ggtggcgcgg cgcctgcgcg gcggcggggt gcgcgacctc accttcgtga ccaactcgct
     4141 caacatcgcc ggtgccctcg aggccaacgg ctggcactcg atcgtggtct ccggcggctc
     4201 cttccggacg ccgtccgacg cgctcgtcgg gccgttcgcc aaccacgtcc tgcgccagct
     4261 caacaccgac ctgctgttcc tg
//
LOCUS       DASSWR010000483         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_837287,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000483 DASSWR010000000
VERSION     DASSWR010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837287"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1595
                     /locus_tag="VFO47_08100"
     CDS             255..1595
                     /locus_tag="VFO47_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HET8788696.1"
                     /translation="MWEALLSEEARRLPAELAAVDAYLDDERFLAPWRALFDRRLGRP
                     SVPIDTLLRLLYLKHRYGLGYESLCREVADSISWRWFCRIGLDRPVPHPTTLIKLVRR
                     AGPEVIEQLNAALVAKLTADKLLRARKLRVDTTVVEGDIDYPTDADLLEHAVRKLGGL
                     VRRIKGRGAASRTRFRDRGRAAGRRMKQLARTLRRRTGVAMAEVDRLTGEVAQIARHT
                     LRQVQAVAANARRARRRRPDDGRLGRLVGELEETITHTGRLLAQTDQRLAGNRVIPDR
                     LVSLADPDVRPIRKGKPQHPTQFGYTLLVAEDERGFIADHHLQQGNPPDAPQLVPVIQ
                     RVIALTGRPPGTVVGDRGFGTAANDQAVQALGVRRVGLQRTGRPGKARLALERTRRFR
                     RLRTWRVGIEARISHLKRAFGLRRTRLRRLGGARTWVGLGIFAYNLQRMTVVAA"
     gene            complement(1687..2256)
                     /locus_tag="VFO47_08105"
     CDS             complement(1687..2256)
                     /locus_tag="VFO47_08105"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET8788697.1"
                     /translation="MLAAALVVTPALSGHAAAGPTAAVGAVVGVVHFSAAAAWFGGLV
                     LLGTCVLPRAEGGLLGAVARFSSVAFTAMVVIVVTGMVQGWRQVGSLQALGNTAYGRL
                     LVVKVAAFLLLIALAGQSRALVRRKLTARVLIGAPDRRGPSAALPGLTDDARALWLLR
                     RLVLAEVIIAIVVLAVTALLGMALPPGAG"
BASE COUNT          384 a          864 c          820 g          294 t
ORIGIN      
        1 tgacttctgg gtcctgcgcg acccctgggg caacgagttc tgcgtgctcc acccgaactt
       61 ccccgagctg ctcgcccgac gccgaccatg gtcacctagc tacttggccc tgaagaagtc
      121 gcgggtgagg tagccggtga gctggggtaa acgccggtgg tcggtgccga tttgtggtcg
      181 aatgacaccg ttgtaacttc cagggccgga ggtggacggt gctgcgtgag accgacccgc
      241 aagcgagcct aactttgtgg gaggcgttgc tgtccgagga ggccagacgg ctgcccgccg
      301 aactcgcagc ggtcgatgcc tacctggatg acgaacgctt cctggcgccg tggcgggccc
      361 tgttcgaccg acggcttggt cgcccctcgg tgccgatcga cacgctgctg cggttgctgt
      421 acctgaagca ccgctacggg ttgggctacg agagcttgtg ccgcgaggtc gccgactcga
      481 tctcgtggcg gtggttctgc cgcatcggcc tggatcggcc cgtgccccat ccgaccacac
      541 tgatcaagct ggtccgccgc gccggcccgg aggtgatcga gcagctcaac gccgcgctgg
      601 tggccaagct gaccgccgac aagctgctgc gggcccgcaa gctgcgggtc gacaccaccg
      661 tggtcgaggg cgacatcgac tacccgaccg acgccgacct gctggagcac gccgtcagaa
      721 agctgggcgg gttggtgcgg cgcatcaagg gccgcggtgc cgccagcagg acccggttcc
      781 gggaccgggg ccgggccgct gggcggcgga tgaagcagct ggcccgcacg ctgcggcggc
      841 gcactggagt ggcaatggcc gaagtggacc ggctcaccgg cgaggtcgcg cagatcgccc
      901 ggcacaccct gcggcaggtg caggcggtgg cggccaacgc ccgccgggca cggcggcgtc
      961 gacccgatga cggacggctg ggccggctgg tcggtgagct ggaggagacg atcacccata
     1021 ccggtcggct gctggcccag accgaccagc ggctggccgg taaccgggtg atccccgacc
     1081 ggctggtgtc gctggccgac ccggacgtcc gccccatccg caagggcaag ccccagcacc
     1141 cgacccagtt cggctacacc ctgctggtgg ccgaggacga acgcggcttc atcgccgacc
     1201 accacctcca gcagggcaac ccacccgacg ccccgcagtt ggtcccagtg atccagcggg
     1261 tcatcgcgct caccggccgg ccgccaggga cggtggtcgg cgaccgcggg ttcggcaccg
     1321 ccgccaacga ccaggcggtg caagccttgg gcgtcagacg ggtcgggctg cagcgcaccg
     1381 gcaggccggg caaggcgcgg ctggccttgg agcggacccg acggttccgg cggctgcgca
     1441 cctggcgggt cgggatcgag gcccgcatca gccacctcaa gcgcgccttc gggctgcggc
     1501 gcacccggct gcgccggctg ggcggcgccc gcacctgggt cggcctgggg atctttgcct
     1561 acaacctgca gcgcatgacg gtggtcgccg catgacccca caggcccggc gccgaccacc
     1621 gatcagcacg gcagcagccc gccacgccta ccaggacttc ttcagggcca agtagctagc
     1681 ctaagcctag cccgccccag gtggcagcgc catgccgagc agcgcggtga cggcaagcac
     1741 cacgatggcg atgatcacct cggcgaggac cagccggcgc agcaaccaca gagctctggc
     1801 gtcgtcggtc aggccaggaa gtgcagcact gggtccacgt cggtctggag cgccgatcaa
     1861 tacccttgcg gtcagcttgc gccggaccag cgcccggctc tggccggcga gcgcgatgag
     1921 gagcaggaac gccgccacct tgaccaccag caggcgccca taggcagtgt tccccagggc
     1981 ctggaggctc ccgacctgcc gccagccctg caccatcccg gtaaccacga tcacgaccat
     2041 ggccgtgaac gccaccgacg agaagcgggc caccgccccc agcagccccc cctctgcccg
     2101 cggcagcaca caggtcccca gcaggaccaa gccgccgaac caagccgcgg ccgccgagaa
     2161 atggacgaca ccgacaacgg cgccaacggc ggcggtgggc ccggccgcgg cgtggccgct
     2221 cagggccggg gtgaccacca gcgcagccgc cagcatcccg accgccaccc aggcgctggt
     2281 gcacgtccac ccggcctccc gcctcgccca ggctcgcaga aaggcaacca ggagcagcag
     2341 cagcaggacg cgcccggccc ag
//
LOCUS       DASSWR010000484         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_329578,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000484 DASSWR010000000
VERSION     DASSWR010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329578"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFO47_08110"
     CDS             complement(<1..1003)
                     /locus_tag="VFO47_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET8788698.1"
                     /translation="MPEPVHVETVIIGGGQAGLAVGRHLARRGRPFLILDANQRVGDA
                     WRRHWDSLRLFTPARYSALAEVPFPGPAHSFPTKDQVADYLEAYATRFQLPVRTGVRV
                     DRLSRNGGRFVVAAGDRRWEADNAVVAMSSFQVPRVPPFAADLGPGVVQLHSAGYRNP
                     SQLQAGGVLVVGAGNSGAEIALEVAGRHPTWLAGRESGQVPFRIDGAAARFVFQPLMF
                     RFVGHRVLTVDKPIGRRMRPRLMAHGAPLVRVKPKDLAAAGVWRVPRVVGVRDGHPQL
                     ADQRVLEVANVIWCTGFGPDFSWIDLPVFGRRCARADAPPRGRHQPAGAVLRGPLLPL
                     "
     gene            complement(1043..2584)
                     /locus_tag="VFO47_08115"
     CDS             complement(1043..2584)
                     /locus_tag="VFO47_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET8788699.1"
                     /translation="MSLATDLGIGVPLEHGLQSTLVAMRLGEVLGVDPETASQTYYAC
                     LLFYVGCTADAEVAADTFGDDDALTTHAMPARFGSTPERMAGMLRALASSGSPAPVRA
                     VQIVRGMPRAAREFKGHVTAFCEVAQMLTDRLGLPASMQGLFAYLVERWDGKGLPGRA
                     RHEEIPLPVRIASVARDAAFQHLLGGAELAAAVVRRRAGGAFDPAVAIPLADAAAELL
                     APDAGASAWEETLAREPGRWLDLEGGAIDRALGAMGEFADLASPYLVGHSVGVAELAT
                     AAARQCRLEAADLERVWRGALVHDLGRVAVPVRIWQKAAPLTPDDWERVRLHAYHSER
                     VLSRSPFLATLTPVASFHHERLDGSGYHRGTGVADIGPPARLLAAADAYHAMTEPRPH
                     RAARSPGEAAEALGREAGEGRLDPDAVSAVLEAAGQRVPRIERPAGLTEREAEVIGLL
                     ARGLQTKQIARALGISVKTADHHVQNAYAKIGVSTRAAAALFAMRHGLVAWGELPIGR
                     VRDRP"
     gene            2540..2848
                     /locus_tag="VFO47_08120"
     CDS             2540..2848
                     /locus_tag="VFO47_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8788700.1"
                     /translation="MLEGHPDPQVRRQRQRRDHLGGTNPFPTRTCHGCHPSDSTRVPE
                     SVKPVRRLLGRLRIGRAGVVGHSSGANIALQLALDAPAMVGSLALLEAWLADVEPFVP
                     "
BASE COUNT          563 a         1404 c         1487 g          487 t
ORIGIN      
        1 gtagaggaag aagaggccca cgaagtacag ccccggctgg ctggtgacga ccccgcggtg
       61 gtgcatcggc tcgcgcgcat cggcgcccga agaccgggag gtcgatccag gagaagtcgg
      121 ggccgaaccc ggtgcaccag atcacgttgg ccacctccag gacccgttgg tcggccagct
      181 ggggatggcc gtcgcgcacc ccgacgaccc tgggaacgcg ccagaccccg gcggcggcga
      241 ggtccttggg cttgacccgg accaggggcg cgccgtgggc catgagcctc gggcgcatcc
      301 tgcggccgat cggcttgtcc acggtcagca cgcgatggcc gacgaaccgg aacatcaggg
      361 gttggaagac gaaccgcgcc gccgcgccgt cgatccggaa cgggacctgg ccggactccc
      421 tgcccgccag ccaggtcggg tgccggccgg cgacctcgag ggcgatctcg gctcccgagt
      481 tgcccgcccc gaccaccagg acgccgcccg cctggagctg ggaagggttg cggtagccag
      541 ccgagtggag ctggacgacg ccgggaccga ggtcggcggc gaagggaggc acccggggga
      601 cctggaagga ggacatggcg accaccgcgt tgtcggcctc ccagcgccgg tcgcccgcgg
      661 cgaccacgaa gcggccgccg ttcctcgaca gccggtccac ccggacgccg gtccggaccg
      721 ggagctggaa ccgggtcgcg taggcctcca ggtagtcggc cacctggtcc ttggtgggga
      781 acgagtgggc cggaccgggg aacgggactt cggccaacgc gctgtagcgg gccggggtga
      841 acagccgcag cgagtcccag tgcctgcgcc aggcgtcgcc gacccgctgg ttggcgtcca
      901 ggatcaggaa cggccgtccc cgccgggcca ggtgacggcc gaccgccagg ccggcctggc
      961 caccaccgat gatcacggtc tcgacgtgca ctggctcggg catggtcgcc tcccgctggt
     1021 cacgctcgct tgccgcctaa cgctacgggc ggtcgcggac gcgtccgatg gggagttctc
     1081 cccaggccac gaggccgtgc cgcatggcga acagggcggc cgcggcgcgg gtggagacgc
     1141 cgatcttggc gtaggcgttc tggacgtggt ggtcggcggt cttgaccgag atcccgagcg
     1201 cgcgggcgat ctgcttggtc tgcaggccgc gggcgagcag accgatgacc tcggcctcgc
     1261 gctcggtcag tccggctggc cgctcgatcc gcgggacccg ttgcccggcg gcctcgagca
     1321 cggcgctgac ggcgtcgggg tcgagccgtc cttcgccggc ctcccggccc agggcctcgg
     1381 cggcctctcc gggcgagcgc gccgcccggt ggggtcgcgg ctcggtcatc gcgtggtagg
     1441 cgtcggcggc ggcgagcagg cgcgcgggcg gcccgatgtc ggcgacgccg gtcccccggt
     1501 ggtagcccga gccgtccagg cgctcgtggt ggaagctggc cacgggcgtg agcgtggcca
     1561 ggaacgggga gcggctgagc acgcgctcgg agtggtaggc atgcagcctg acccgctccc
     1621 agtcatccgg cgtgagcggc gccgccttct gccagatccg gaccgggacg gccacccgtc
     1681 cgagatcgtg caccagcgcg ccccgccaga cccgctccag gtcggcggcc tccagacggc
     1741 actgccgggc cgccgccgtg gccagctcgg ccacaccgac cgagtggccg accagatacg
     1801 gcgaggcgag gtcggcgaac tcccccatgg ccccgagggc ccggtcgatc gccccgccct
     1861 ccaggtccag ccatcggccc ggctcgcggg cgagcgtctc ctcccaggcg ctcgcaccgg
     1921 cgtcgggcgc cagaagctcg gcggccgcgt cggcgagcgg gatggccacg gccgggtcga
     1981 acgctcctcc cgcccgcctg cggaccaccg ccgcggcgag ctcggcgccg ccgagcaggt
     2041 gctggaacgc ggcgtcgcgg gccaccgagg cgatccgcac cggcagcggg atctcctcgt
     2101 gccgggcgcg accgggcagg cccttgccgt cccagcgctc gaccaggtag gcgaacaggc
     2161 cctgcatgga cgcgggtagg ccgagccggt cggtcagcat ctgggccacc tcgcagaacg
     2221 cggtgacatg gcccttgaac tcccgggccg ccctcggcat gccgcgcacg atctgcacgg
     2281 cccgcacggg tgcagggctg cccgagctgg caagcgcccg cagcatcccg gccatccgct
     2341 cgggcgtcga cccgaacctg gccggcatgg catgggtcgt cagggcatcg tcgtcgccga
     2401 aggtgtcggc ggcgacctcc gcgtcggcgg tgcagccgac gtagaacagc aggcacgcat
     2461 agtaggtctg cgacgccgtc tccgggtcga cgcccagcac ctcgccgagc cgcatggcca
     2521 ccagcgtgct ctgcaggcca tgctcgaggg gcaccccgat ccccaggtcc gtcgccagcg
     2581 acaacgccgc gatcacctcg gcggcacgaa cccgttcccc acccggacct gccatggctg
     2641 ccacccatcg gacagtacgc gagtcccgga gtctgttaaa ccggtacgtc ggctgctggg
     2701 ccggctcagg atcggccggg cgggcgtggt cggccattcc tccggggcca acatcgccct
     2761 gcagctggcc ctggacgccc cggccatggt cggctcgctg gccctgctcg aggcctggct
     2821 ggccgacgtc gagccgttcg tgccttgacg gcgaacggct ccggctggct ggccaggtgg
     2881 aacgggacga tggtcacgtt gacgttctcc atcgagccca gcaccttggt cagcccggcc
     2941 gaggtctggt cgtgcaggac ccggcgccag aagccgacgt agcggcggcg cgggaccagg
     3001 accgtgacct cggtgtcggg ccggacccgc tcggcgatcg cctcctcgac ggtggccagc
     3061 aggttgcggt caggggcgtc gaccagctcc agcgggaacg ggatttggac cttcgaccac
     3121 agccggccca gctcccgggc gtggtcgggg tcgacggcga cgtgcagggc ggtgatgctg
     3181 agcgggttga gctggcgggc gtactggagg gcgctgaggg cggcctggtc gaggtcctcg
     3241 atcagcacca ccacctcgtg gcggggcttg ggcggggcca ggcgctggct cgcctggacc
     3301 ttcagctggg ccagctcgcg gccgtaggtc cgctggatgc ggagcagcag ccagaccagc
     3361 agcgggatgg cgatcacgac catccaggcg ccctcggcga acttgccgac gacgatgacc
     3421 accagggcga ccagggtcac ggcggccccg accccgttga tcaggacgcc gccgcgccag
     3481 cccggctcgc gcagccgcag gtggcggcgg gtcatgcccg cctgggcgag ggtgaaggag
     3541 gtcaccaccc ccaccgcgta cagcgggatc agcgagtggg tgtcggcctg gaagaccacg
     3601 atcaggaaga tggccaccgc cgccagggcg aggatgccgt tggagtagac cagccggtgg
     3661 ccgcgcttgc gcagctggcg gggcagcagg gcgtcgccgg aggcgaagct ggccagcagg
     3721 gggaacccgg agaaggcggt gttggccgcc agcaccagga tgagcagggt ggccgcctgg
     3781 acgaggagga acgccggccc gccgcccagg acctgccggg cgatctggga gatgaccgtc
     3841 gggtagccgc tggagaaggg cagcgcctcc agccgcacgg ccaggtaggt gatgccacag
     3901 aacagggtcc ccatgatcgc cgccatcatc atcagggtct t
//
LOCUS       DASSWR010000485         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_894487,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000485 DASSWR010000000
VERSION     DASSWR010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894487"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFO47_08125"
     CDS             complement(<1..713)
                     /locus_tag="VFO47_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788701.1"
                     /translation="MTAVNPTPVASVRVPAAGLRHQVRGAAVVWQREMIRFGRDRSRI
                     VSSLVQPVLFLFVLGTGLSSLIASNGDVDFRTFLFPGVLATSVLFTAAFSGISMVWDR
                     EFGFLREMLVAPVSTTAILTGKCLGGATVATLQSLIILALAPLVGVPYNLLMMVRLVG
                     LLFLMAFMICALGLFLSARVKQVQSAMPLVQLTITPLMFLSGSLFPLSNLPGWLHVAT
                     TLNPMTYAVEPIRSTVFDHL"
     gene            complement(719..1699)
                     /locus_tag="VFO47_08130"
     CDS             complement(719..1699)
                     /locus_tag="VFO47_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788702.1"
                     /translation="MENSAAAITIRGLVKRFKQVEAVAGINLDVRPGETFGFLGPNGA
                     GKSTTIKILCTLADATEGTARVAGFDVARERDQVRRHIGLVFQEPTLDAYLTAEQNLR
                     FHGELYGVDRATAAQRRQEVLTMVDLWERRGDLVQTFSGGMRRRLEIARGLLHSPRVL
                     FLDEPTIGLDPQTRASIWDYLEELHRRQEITVFVTTHYMDEAEHCDRIAIVDQGRIVA
                     LDTPAALKSGIGKDRVQITTDDDARAIEELAARFEIEAAVHEGAVTFAVADGAAFVPR
                     LFAGLDVPINSVTVTRPSLDDVFLAYTGRTIRDAEAARPMNPMAFAARGR"
     gene            1778..>2297
                     /locus_tag="VFO47_08135"
     CDS             1778..>2297
                     /locus_tag="VFO47_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET8788703.1"
                     /translation="MTLTQEQVVKRAVDLMAEAGLEALTLRRLATELGVSAPTLYWHV
                     RNKRELLDLMAEALVDRAGRATSPAPGQPWWEWLAERARLQFRALISHRDAALVVAGN
                     RPTEASLPDIEEVLDSLVAVAVPPAEALRVILSIGNYVIGCAAERQAEEARTADPERD
                     TRLLEQAAALPNL"
BASE COUNT          356 a          770 c          859 g          312 t
ORIGIN      
        1 aggtggtcga acaccgtgga gcggatcggc tccacggcat aggtcatcgg gttgagggtg
       61 gtggccacgt gcagccagcc gggcaggttg gagaggggga acagcgaccc ggagaggaac
      121 atcagcgggg tgatggtcag ctggaccagc ggcatggccg actggacctg cttgacccgg
      181 gcggacagga acagcccgag ggcgcagatc atgaaggcca tcaggaacag cagcccgacc
      241 agcctgacca tcatcagcag gttgtagggc accccgacca ggggggccag ggcgaggatg
      301 atcaggctct ggagggtggc cacggtggcc ccgcccaggc acttgccggt gaggatggcg
      361 gtcgtgctca cgggggcgac cagcatctcc cggaggaagc cgaactcccg gtcccagacc
      421 atcgagatgc ccgagaacgc cgccgtgaac agcaccgagg tggccagcac gcccgggaac
      481 aggaaggtcc ggaagtcgac gtcgccgttg gaggcgatca gcgacgacag gccggtgccg
      541 agcacgaaca ggaacagcac cggctggacc agcgaggaga cgatccggga gcggtcgcgg
      601 ccgaagcgga tcatctcccg ctgccagacc acggcggccc cgcggacctg gtggcggagg
      661 ccggccgcgg gcacgcgcac ggacgccacc ggggtcgggt tgacggcggt catctcactc
      721 acctcccccg ggccgcgaag gccatcgggt tcatcgggcg ggcggcctcg gcgtcgcgga
      781 tggtccggcc ggtgtaggcc aggaagacgt cgtcgaggct gggccgggtg acggtgaccg
      841 agttgatggg cacgtccagg ccggcgaaca gccggggcac gaacgccgcc ccgtcggcca
      901 cggcgaaggt caccgccccc tcgtggacgg ccgcctcgat ctcgaagcgg gcggccagct
      961 cctcgatcgc ccgggcgtcg tcgtcggtgg tgatctgcac ccggtccttg ccgatgccgg
     1021 acttgagggc ggccggggtg tccagggcga cgatgcggcc ctggtcgacg atggcgatgc
     1081 ggtcgcagtg ctcggcctcg tccatgtagt gggtggtgac gaacacggtg atctcctggc
     1141 ggcggtgcag ctcctccagg tagtcccaga tggaggcgcg ggtctggggg tcgaggccga
     1201 tggtgggctc gtccaggaac agcacccgcg gcgagtgcag caggccccga gcgatctcca
     1261 gccgccgccg catgccgccc gagaaggtct ggaccaggtc gccgcgccgc tcccacaggt
     1321 cgaccatggt caggacctcc tggcggcgct gggcggcggt ggcccggtcg accccgtaca
     1381 gctcgccgtg gaacctgagg ttctgctcgg cggtcaggta ggcgtccagg gtcggctcct
     1441 ggaagaccag gccgatgtgg cggcggacct ggtcgcgttc gcgggccacg tcgaagccgg
     1501 ccacccgggc cgtgccttcg gtggcgtcgg ccagggtgca gaggatcttg atggtggtcg
     1561 acttcccggc gccgttgggc ccgaggaagc cgaaggtctc ccccggccgc acgtccaggt
     1621 tgatgccggc cacggcctcg acctgcttga agcgcttgac cagtccccgg atggtgatgg
     1681 ccgccgcgga gttctccatt ggcacctcac ttctctaacg ttgttcggga tgctactctt
     1741 acgccgttag agcggcaagc gggaggaggc ggcggcggtg accctgaccc aggagcaggt
     1801 ggtcaagcgg gccgtcgacc tgatggccga ggccggactg gaagctctga ccctgcgtcg
     1861 gctggccacc gagctggggg tgtcggcacc gaccctctat tggcatgtcc gcaacaagcg
     1921 cgagctgctc gacctgatgg ccgaggccct ggtcgaccgg gccggccgcg ccacctcccc
     1981 ggcaccgggg cagccctggt gggagtggct ggccgagcgg gcccggcttc agttccgggc
     2041 cctcatctcc catcgcgacg ccgccctggt ggtcgccggc aaccggccca ccgaggccag
     2101 cctgcccgac atcgaggagg tgctggacag cctggtcgcc gtcgcggtcc cccccgccga
     2161 ggccctgcgc gtcatcttgt cgatcggcaa ctacgtgatc ggctgcgccg ccgagcgcca
     2221 ggccgaggag gccaggaccg ccgaccccga gcgcgacacc cgactgctgg agcaggcagc
     2281 ggccctgccc aacctcc
//
LOCUS       DASSWR010000486         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_160209,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000486 DASSWR010000000
VERSION     DASSWR010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160209"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFO47_08140"
     CDS             <1..391
                     /locus_tag="VFO47_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET8788704.1"
                     /translation="DGMLDPEPGVPLTAMGYEFWPQALGATIRRAAETTGAPVLVTEN
                     GIAADDDRQRVEFVTLALESVLDCLADGIDVRGYTYWSLLDNFEWAHGYRPTFGLVAV
                     DRTTQVRTPKPSAAWLGRIARANRLTR"
     gene            527..1783
                     /locus_tag="VFO47_08145"
     CDS             527..1783
                     /locus_tag="VFO47_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HET8788705.1"
                     /translation="MMDETNWAGNYRYQATRLHRPSTLEQLQEVVAGAPRIRVLGSRH
                     SFTGIADSAELVSLQGLPSDVTVDRDAGTVTCPAGLTYGELALALQREGVALANLASL
                     PHISVAGAVATATHGSGDANGNLATAVAGLELVTSSGELLQASRGDPDFDGLVVGLGA
                     LGAVTRLTLDVEPAYQVRQRVFEGLAWDALHEHFDEITAAGYSVSVFTRWAEAVDQVW
                     VKRRVTDDPEAVDDDLFGATAATVDRHPIIELDPVNCTPQLGAPGRWSDRLPHFRMGF
                     TPSSGAELQSEYLIPRHHAATAIGAVHALADVVRPVLQVSEVRTVAADRLWMSPQYRQ
                     ATIAIHFTWHPDRAGVERALAELEAALAPCQARPHWGKVFLADATTIAPLYERLGDFR
                     RLADRLDPRGAFRNPWLIDHVLGPRA"
     gene            1794..>2965
                     /gene="maeA"
                     /locus_tag="VFO47_08150"
     CDS             1794..>2965
                     /gene="maeA"
                     /locus_tag="VFO47_08150"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HET8788706.1"
                     /translation="MYERPYELGRPEDGYPVRIRARGNEVLATPMINRGTAVTLEERE
                     TLGLTGLLPGGVSTIDGQLRRVYAQYLRQPDDLAKNVYLAHLRDRNEVLFYRLLTERI
                     EEMLPIVYTPTVGIAIERFSHEYRRPRGVYLSVDRPQDVETALGNYNRGGDAVDLIVA
                     TDSEGILGIGDQGVGGIDIAIGKLAVYTAAAGIHPRRVIPVVLDMGTDNLALLNDEMY
                     LGNRHPRVRGERYDALIDAYVTAATKLFPQAMLHWEDFGASNARRILNRYADQVCTFN
                     DDMQGTAAVVLAAAFSAVRAAGQRMRDQRVVIHGAGTAGLGIADMLRDVMVREGLPDE
                     EAARRFYPLGRNGLLTDDQTAGMYDFQVPYARPAGEIAGWSTAAGSDGKSLADVVA"
BASE COUNT          434 a         1143 c         1010 g          378 t
ORIGIN      
        1 ggacgggatg ctcgaccccg agccgggggt gcccctgacc gccatgggct acgagttctg
       61 gccccaggcg ctgggggcga ccatccgccg ggccgccgag accaccggcg cgcccgtgct
      121 ggtcaccgag aacggcatcg ccgccgacga cgaccgccag cgcgtcgagt tcgtcaccct
      181 ggccctggag agcgtcctag actgtctggc cgacgggatc gacgtgcgcg gctacaccta
      241 ctggagcctg ctcgacaact tcgagtgggc ccacggctac cggcccacct tcggcctggt
      301 cgcggtcgac cggaccaccc aggtccgcac ccccaagccg agcgcggcct ggctcggccg
      361 gatcgcccgg gcgaacaggc tcacccgtta gctggccagg cgcggggggc cggacagcgc
      421 cgggactcct gtgggcccgg ctcgtcccgg cggtagttgc cggtcagcag ccccgagacc
      481 gcatcgatcc ctcccgctcg gcgccgcaac caaggagact agcccgatga tggacgagac
      541 gaattgggcc gggaactacc ggtaccaggc caccaggctg caccggccgt ccacgctgga
      601 gcagctccag gaggtcgtcg ccggcgcgcc ccggatccgg gtgctcggct ccaggcactc
      661 gttcaccggc atcgccgact ccgccgagct ggtcagcctg cagggcctgc ccagcgacgt
      721 caccgtcgac cgcgacgccg gcacggtcac ctgcccggcc ggcctcacct acggggagct
      781 ggccctggcg ctccagcggg agggggtggc cctggccaac ctggcgtcgc taccccacat
      841 atcggtcgcg ggggccgtgg ccaccgccac ccacggctcc ggggacgcca acggcaacct
      901 ggccaccgcc gtcgccggcc tggagctggt cacctcctcc ggcgagctcc tccaggcgtc
      961 cagaggcgac ccggacttcg acggtctggt cgtcgggctg ggcgcgctcg gggcggtcac
     1021 ccgtctgacg ctcgacgtcg agcccgccta ccaggtccgc cagcgggtgt tcgaaggcct
     1081 cgcctgggac gccctgcacg agcacttcga cgagatcacc gccgccggct acagcgtgag
     1141 cgtgttcacc cgctgggccg aggcggtcga ccaggtgtgg gtcaagcgcc gggtcaccga
     1201 cgaccccgag gcggtcgacg acgacctgtt cggcgcgacc gcggccaccg tcgaccgcca
     1261 cccgatcatc gagctcgacc cggtcaactg caccccgcag ctcggtgccc ccggccgctg
     1321 gtcggaccgg ctgccgcact tccgcatggg cttcaccccc agcagcggcg ccgagctcca
     1381 gtccgagtac ctgatccccc gccaccacgc cgccaccgcc atcggcgccg tccacgccct
     1441 cgccgacgtg gtccggccgg tgctgcaggt cagcgaggtc cgcaccgtgg ccgccgaccg
     1501 cctgtggatg agcccccagt accggcaggc cacgatcgcc atccacttca cctggcaccc
     1561 cgaccgggcc ggggtcgagc gggcgctcgc cgagctggag gccgccctcg ccccctgcca
     1621 ggcgcgaccc cactggggca aggtgttcct ggccgacgcc accaccatcg cccccctgta
     1681 cgagcgcctg ggtgacttca ggcgcctggc cgaccggctc gacccgcggg gcgcgttccg
     1741 caatccttgg ctgatcgacc acgtcctcgg accccgtgcg tagaggagac gacatgtacg
     1801 agaggccgta cgagctgggt cgccccgagg acgggtaccc ggtccggatc cgggcccgcg
     1861 gcaacgaggt gctggccacg ccgatgatca accggggcac cgcggtcacg ctggaggagc
     1921 gcgagacgct ggggctgacc gggctgctgc caggcggggt gtccacgatc gacggccagc
     1981 tgcgccgggt ctacgcgcag tacctgcgcc agcccgacga cctggccaag aacgtgtacc
     2041 tggcccacct ccgcgaccgc aacgaggtgc tgttctaccg gctgctcacc gagcgcatcg
     2101 aggagatgct cccgatcgtc tacaccccga ccgtcggcat cgcgatcgaa cggttcagcc
     2161 acgagtaccg gcggccgcgc ggggtctacc tgtccgtcga ccggccgcag gacgtcgaga
     2221 cggcccttgg caactacaac cgcggcggcg acgccgtcga cctgatcgtg gccaccgact
     2281 ccgagggcat cctcggcatc ggggaccagg gggtcggcgg gatcgacatc gcgatcggca
     2341 agctggccgt gtacacggcc gcggccggga tccatccgcg ccgggtgatc ccggtcgtcc
     2401 tggacatggg caccgacaac ctggccctgc tcaacgacga gatgtacctg ggcaaccggc
     2461 accccagggt ccgcggcgag cgctacgacg cgctcatcga cgcctacgtg acggcggcga
     2521 ccaagctgtt cccccaggcg atgctgcact gggaggactt cggcgccagc aacgcccggc
     2581 ggatcctgaa ccgctacgcc gaccaggtct gcaccttcaa cgacgacatg cagggcaccg
     2641 ccgcggtggt cctggcggcc gcgttctccg cggtccgggc ggccggccag cgcatgcgcg
     2701 accagcgggt ggtcatccac ggcgccggca ccgccggcct gggcatcgcc gacatgctgc
     2761 gcgacgtcat ggtccgcgag ggcctccccg acgaggaggc ggcccgccgc ttctacccgc
     2821 tcggccgcaa cggcctgctg accgacgatc agaccgcggg catgtacgac ttccaggtgc
     2881 cgtacgccag gccggccggc gagatcgccg gctggtcgac cgcggccggc agtgacggca
     2941 agagcctcgc tgatgtcgtc gccaa
//
LOCUS       DASSWR010000487         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_583984,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000487 DASSWR010000000
VERSION     DASSWR010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583984"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFO47_08155"
     CDS             complement(<1..968)
                     /locus_tag="VFO47_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8788707.1"
                     /translation="MVGSPKQRALLAMLVVHLNRVVPLDAIIEELWGERLPASASASV
                     QSLVSRLRRSLSDLWPEAGSRLRGREPGYVLEGDPAQVDANRFEELLARGREAVERGE
                     PEAATDALERALALWRGPALADLADRRFARLEASRLEEARLAAVEELAEAELALGHPA
                     QGLARLEPHVAEHPLRERAWGQIMVALYRLGRQADALSAYRRIRKILREELGLEPTPS
                     LRQLEQQILLQRPELEGSRPPEPVRPAGQATRPSGTTRPGDTVVFLFTDIEASSHRWE
                     GDEEAMAEDLARHDEIVLGAVEEAQGRVFANTGDGLCAAFPTASAAL"
     gene            1220..2023
                     /locus_tag="VFO47_08160"
     CDS             1220..2023
                     /locus_tag="VFO47_08160"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET8788708.1"
                     /translation="MDSTTVLSQARSALEATARRTAGLIRSLPDLDGRIPGSEWTVRE
                     AAVHLVNHAGLHTEIAEGLASPIERVAREALAAENAARIADIPEAGAEKVASLLTDAV
                     AGLLEATANRSGHQPVQFHAGVSLDLAALVCISLGEQLLHGYDIATALGAPWPIRPGH
                     ADLVIHGYGPYYESVVDPEMARGVTASFGIDLRGGSSFAVRFNDGAYRLEPPRSGPVD
                     STISADPVAFLLMWSGRMTLWEAIALNLYVADGKDSRSGLHFMELFEYP"
     gene            2156..2857
                     /locus_tag="VFO47_08165"
     CDS             2156..2857
                     /locus_tag="VFO47_08165"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET8788709.1"
                     /translation="MVTGTRARSYEEMVDALAEEGLPALQGKLSDLTAQDWERPTLLR
                     PPDADKPPWTVLQLAAHFDVFMGLTMPLVAEPMSDQPVVDRASFYISVSDRSKVSPVI
                     YQYIVDHAQGHTPATIRDQVGKTFKQALEAIRTTPPDTIGPGFFGPMRLDEFVATRLV
                     ETRVHGLDLTDALGAPPLHMPKTTTMAAEVLDEVLARRAVPGRPADLEGDDLGFIRAA
                     AGRGEYPDPRLPVVG"
BASE COUNT          437 a         1054 c         1006 g          450 t
ORIGIN      
        1 agcgcggccg aggccgtggg gaaggcggcg cacaggccgt ctccggtgtt ggcgaacacc
       61 cggccctggg cttcctccac ggcgcccagc acgatctcgt cgtgacgggc caggtcttcc
      121 gccattgcct cctcgtcgcc ctcccaccga tgcgagctgg cctcgatgtc ggtgaagaga
      181 aagaccaccg tgtcgcccgg gcgggtcgtt ccggagggcc gcgttgcttg tcccgccggc
      241 ctcaccggct ctggtgggcg ggatccctcg agctcggggc gttggaggag gatctgctgc
      301 tcgagctggc ggagggaagg cgtgggctcc aggccgagtt cctcacgaag gatcttcctg
      361 atccgccggt aggcgctcag ggcgtcggcc tggcggccga gccggtaaag ggccaccatg
      421 atctgccccc aggcccgctc ccgtagtggg tgctcggcga cgtgaggctc caggcgggcc
      481 agcccctggg cgggatggcc gagtgccagt tcggcctcgg ccagctcctc gacggcggcg
      541 aggcgggcct cctccagccg gctggcctcg aggcgggcga agcgccgatc ggcgaggtca
      601 gcgagggctg ggccccgcca cagggccagg gcccgctcca gggcatcggt ggcggcctca
      661 ggctcgcctc gctcgacggc ctcccggcct cgggcgagca gctcctcgaa acggttcgcg
      721 tccacctggg ccggatcccc ttcaaggacg tagcccggct cccggcctcg caggcggctt
      781 cccgcctccg gccagaggtc ggagaggctc cgccggagac gggacaccag gctctggaca
      841 ctggcgctgg cgctggccgg gagccgctcg ccccagagct cctcgatgat cgcgtcgagg
      901 ggaaccacgc ggttgaggtg gacgacgagc atggccagca gcgctcgctg cttggggctg
      961 ccgaccatcg ccacccgtcc gtcggcggcg acctcgaacg cacccagcag gtggaactcg
     1021 agaacctgat gctccgcagt tgagagcagt tgggtccccc cctccagtgg ccgtggctgc
     1081 agcgtacgac atgcccgttc gtcctgaaca gggcaatggg gagccgcgtc agcgacgcgc
     1141 aagcccggcg taaggagcgt cgagcatcct tcccctaggc gtcgccgccg actggagcat
     1201 ccccaggaga ggaggacgaa tggactcgac gacggtcctg tcgcaggccc gctcagcgct
     1261 ggaggcgacc gccaggcgaa cggccgggct catccgctcc cttcccgatc tggacgggcg
     1321 gatcccgggc tctgagtgga ccgtgcgcga ggccgccgtc cacctcgtga accacgccgg
     1381 gctccacacc gagatcgccg agggcctcgc ctccccgatc gaacgtgtgg ccagggaggc
     1441 actggcggcc gagaacgccg cccggatcgc cgacatccca gaggccggcg ccgaaaaggt
     1501 cgcctccctt ctcaccgacg cggtggccgg gctccttgag gccaccgcca accgctccgg
     1561 ccaccagccg gtccagttcc acgctggcgt gtccctcgac ctggctgccc ttgtctgcat
     1621 cagcctcggt gagcagctcc tccacggcta cgacatcgcg accgccctcg gtgccccgtg
     1681 gcccatcaga cctggtcatg ccgacctcgt gatccacggg tacggcccct actacgagag
     1741 cgtggtggat cctgaaatgg ctcgcggagt cacggccagc ttcggcatcg acctgcgtgg
     1801 cggctcgtcc ttcgcggtgc ggttcaacga cggtgcctat cggttggaac cgcctcgctc
     1861 ggggcccgtc gactccacga tctcggccga tccagtcgcg ttcctcctca tgtggtcggg
     1921 tcggatgacc ctttgggagg ccatcgccct gaacctctat gtcgccgacg ggaaggactc
     1981 caggtcgggt ctgcacttca tggagctctt cgaatatcca tgaccagagc atcggaagga
     2041 ggaatccagg caattagccc cgcatgatcg agcaccagga actcccatcg tctgtcagct
     2101 tcggaccggc aggttccggt ccagccggcg ggcggcgggc tccaggaggt ggagcatggt
     2161 gaccggcacg agagctcgct cctacgaaga gatggtggac gccctcgccg aggagggcct
     2221 cccggcgctg caaggtaagc tgtcggacct cactgcccag gactgggagc ggcccaccct
     2281 gctgcggcca cccgatgcgg acaagccgcc gtggacggtg ctgcagctgg ccgcccattt
     2341 cgacgtcttc atgggcctga ccatgccgct ggtggccgag cccatgtcgg accagccggt
     2401 ggtggaccgg gccagcttct acatctcggt ctctgaccgc agcaaggtct ccccggtcat
     2461 ctaccagtac atcgtcgacc acgcccaggg ccacaccccg gccaccatcc gcgaccaggt
     2521 cgggaagacg ttcaagcaag ccctggaggc gatcaggacc acaccccccg acaccatcgg
     2581 gcccggtttc tttgggccga tgcggctgga tgagttcgtg gccacccggc tggtggagac
     2641 ccgggtgcat ggcctggacc tgaccgacgc cctgggggca cccccgctgc acatgccaaa
     2701 gaccacgacg atggcggccg aggtgctgga tgaggttctc gcccggcggg ccgtccccgg
     2761 gcgtccggct gatctggagg gcgatgacct ggggttcatc cgggccgccg ctggacgcgg
     2821 tgagtacccc gatccccgcc tcccggtcgt gggatgacgc acacgcccac gagctgctac
     2881 cggtaagggg ccgggcgcgg tcccggcccg taccgcgctt gcttccatcc agggtcgttg
     2941 cccgcac
//
LOCUS       DASSWR010000488         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_357584,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000488 DASSWR010000000
VERSION     DASSWR010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357584"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFO47_08170"
     CDS             <1..212
                     /locus_tag="VFO47_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788710.1"
                     /translation="PGGIVVVDGAMWRARLATGEGSLGTGQRIRVSSVVEGILQVEPE
                     DPSAVRPSRRRGRRTTPVAEPASQE"
     gene            219..1154
                     /locus_tag="VFO47_08175"
     CDS             219..1154
                     /locus_tag="VFO47_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788711.1"
                     /translation="MSRQEDIRDDPQGDARAGRPGTPDRRLVALQLVAGLAVIAFGWG
                     TSLGEVDPFRGYWFDLVWTGFILAADAVVWARAGHSLIHGGGWRLAAMFALSAPFWWA
                     FEIANWRLENWKYVGTSVYGGQAHVVLKTISFLFVLPALAESRDLLRSFVRFPHPPAI
                     PLPSWTATALVVLGLACVPLLYLFPDQTFPLVWLAPLAVLDGIAELRGRPSVIGLVRQ
                     GRAGPVLLLAAAGLGTGILWEMWNWGAVPFWQYRIPYVGFWPVFEMPILGYLGYLPFI
                     LAADAFWRLFTGGPGGLTEGPVTDLGQDGPAGHRV"
     gene            1207..2067
                     /locus_tag="VFO47_08180"
     CDS             1207..2067
                     /locus_tag="VFO47_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET8788712.1"
                     /translation="METWVVVLIVIAVIALIVWGVVSMVHVLQEYERGVVFRLGRVQR
                     KAKGPGLIILLPFGIDRMRTVTIQTVAMNVPPQDVITKDNVTMRVDAVVYARVEEPVA
                     AVVKVQNYLFAVSQAAQTNLRAMLGKYDLDTLLAEREAVNRELHGVIARVTHDWGVEV
                     LSVEVKDVDLPDDLRRAMAREAEADRDARALLISADAERKASRVLADAAAVLSTNPQA
                     MQIRFLQTVTAVATEQNSTLVMPIPVELLNLFGAGRPAGDPSNGRAVEPPRPGPEDER
                     PPEQGNGRPG"
BASE COUNT          291 a          804 c          799 g          332 t
ORIGIN      
        1 acccgggcgg catcgtggtc gtcgacgggg ccatgtggcg ggcccgtctg gccaccggcg
       61 agggcagcct cggcaccggc cagcgcatcc gggtcagctc ggtggtcgag ggcatcctcc
      121 aggtcgagcc cgaggacccc tcggccgtgc ggccgtcacg ccgacgcggc cgccggacca
      181 cccccgtcgc cgagccggcc agccaggagt agccgggcat gagcaggcag gaggacatcc
      241 gggacgaccc ccagggggat gcccgggccg gtcgacccgg cacccccgac cggcggctgg
      301 tggccctcca gctggtcgcc gggctggccg tcatcgcctt cggctggggg acgagcctgg
      361 gcgaggtcga ccccttccgc ggctactggt tcgacctggt ctggaccggg ttcatcctgg
      421 ccgcggacgc ggtggtgtgg gcgcgggccg gccactcgct catccacggc ggcggctggc
      481 ggctggcggc catgttcgcc ctcagcgcgc cgttctggtg ggcgttcgag atcgccaact
      541 ggcgcctgga gaactggaag tacgtcggca cctcggtcta cggcggccag gcccacgtcg
      601 tcctgaagac catctcgttc ctgttcgtgc tcccggccct ggccgagtcg cgcgacctgc
      661 tgcggtcctt cgtgcggttc ccgcacccgc cggccatccc gctcccctcc tggaccgcga
      721 ccgccctggt ggtgctcggc ctggcctgcg tgcccctgct gtacctgttc cccgaccaga
      781 ccttcccgct ggtctggctg gcccccctgg ccgtcctgga cgggatcgcc gagctgcgcg
      841 gccggcccag cgtcatcggg ctggtccgcc agggccgggc cgggccggtg ctgctcctgg
      901 ccgccgccgg cctcggcacc gggatcctgt gggagatgtg gaactggggc gcggtgccct
      961 tctggcagta ccgcatcccc tacgtcggct tctggccggt gttcgagatg ccgatcctcg
     1021 gctacctcgg ctacctgccg ttcatcctcg ccgcggacgc gttctggcgc ctgttcaccg
     1081 ggggcccggg cggcctcacc gagggcccgg tcaccgatct gggccaggac ggcccggccg
     1141 gccaccgggt gtgacaaact agccacagca ttccggaatc ggacgacctg gacgggaggg
     1201 acggccatgg agacctgggt ggtcgtcctg atcgtgatcg cggtgatcgc cctgatcgtg
     1261 tggggcgttg tctccatggt ccacgtgctc caggagtacg agcgcggggt cgtgttccgg
     1321 ctcggccggg tccagcgcaa ggccaagggg cccggcctga tcatcctgct gccgttcggg
     1381 atcgaccgga tgcgcacggt caccatccag acggtggcca tgaacgtccc gccccaggac
     1441 gtgatcacca aggacaacgt caccatgcgg gtcgacgcgg tcgtctatgc ccgggtggag
     1501 gagccggtcg cggccgtcgt caaggtgcag aactacctgt tcgcggtcag ccaggccgcc
     1561 cagaccaacc tccgggccat gctcggcaag tacgacctgg acacgctgct cgccgagcgg
     1621 gaggcggtta accgggagct gcacggggtc atcgcccggg tgacccacga ctggggggtc
     1681 gaggtgctgt cggtcgaggt caaggacgtc gacctgcccg acgacctgcg gcgggccatg
     1741 gcccgcgagg ccgaggccga ccgggacgcc agggccctgc tgatctcggc cgacgccgag
     1801 cggaaggcct ctcgggtgct ggccgacgcg gccgccgtcc tgagcacgaa cccccaggcg
     1861 atgcagatcc gcttcctcca gacggtcacg gccgtggcca ccgagcagaa ctcgaccctg
     1921 gtcatgccga tcccggtcga gctgctgaac ctcttcgggg ccggccgccc ggccggcgac
     1981 cccagcaacg gccgcgccgt cgagcccccg cgaccgggcc cggaggacga gcgaccgccc
     2041 gagcagggca acggccgccc cggctagcac ctttacgccg ggttgaagct ggtcgtggga
     2101 tacctgggcc gcaacaaaag cgttactgcc tggtgattga ggctgcgggc gccctgggat
     2161 acgctgcgat ccggcagggt aaggaaggaa cattcggcat ggccacctca tcgggccccg
     2221 cggctg
//
LOCUS       DASSWR010000489         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1148270, whole genome shotgun sequence.
ACCESSION   DASSWR010000489 DASSWR010000000
VERSION     DASSWR010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148270"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1192
                     /gene="galT"
                     /locus_tag="VFO47_08185"
     CDS             113..1192
                     /gene="galT"
                     /locus_tag="VFO47_08185"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET8788713.1"
                     /translation="MVKTSIRLADGRELLYFDEREGVDRHVPDTRDLTAAVTSSQIRC
                     DPLTEEWVVVASHRQGRTHLPPADQCPLCPSREGRATEIPAADYDVVVFENRFPSLAP
                     DPGPVADGGPFHRQPGVGRCEVVCFTADHGGALARLPVGRIRTVVEVWADRTLELGAL
                     DGVEQVFPFENRGEEIGVTLHHPHGQIYAYPFVAPRTARQLDVARAYRDATGECRSCS
                     IVAAEADGERVVATAPGWLAFVPFGARWPFEVHLFPTRHLPDLPALGDDERDGLAALL
                     KDVLGRFDALFDQPLPYMLTCHQAPVRRDRDLAHAHLEAFSIRRTATKLKYLAASESG
                     AGVFINDIAPEQAAELLRAAMPHSR"
     gene            1316..3586
                     /locus_tag="VFO47_08190"
     CDS             1316..3586
                     /locus_tag="VFO47_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET8788714.1"
                     /translation="MIRAVVSPGGRQRPGRTTLAAGLLLALCLAGIALAPRAGAAPSP
                     ATYTNPVSRDFADTFADPAVIKAKDGWWYAYGTTDPLREGERTRHLIPTARSADLVNW
                     TYVGDAFTESTLPAWAAPDAALWAPDIRYLDGTYYLYYVVTQTTVTDEPNDNAIGVAT
                     APTPTGPWTDSGEPVVGPRRGDSGNPGDFKWTFDPSQFTDIDGTRYLYYGSYYGGIFV
                     TELNEDATRVVGTPTMVAIDNRYEGAYVVRHGRWYYLFASEANCCAGPTTGYTVFAGR
                     SASPLGPFTDRDGISLLTSRVGGTIVVTPNGNTWVGTGHNAVVTDLAGQDWFVYHAID
                     RRDPFLDEPFGINERPMLLDRLDWIDGWPTVRAGRWASEGPQRAPVTDWTVALPDGAL
                     DPPADSCEPTFEVTAGRSPDDHRAEGDLRLGEGATAAGLVTSWRSHRNYVVSWLDREA
                     GALVTDVVRGGRSLGREASPLPAGFRHDTWHNVAAELRGRVLTVEVTDARLHDPVAVQ
                     RRVLPHGTGGAGAVGAAARCGPAEAANLGAARLYDPVRHRVPPPRVGELVYADEFGDG
                     TVGGPGWSWVRGPDPAVTETGGALRWPTQPGDLVGPDETASVLLRDAPAGAYTVETRL
                     AIDLGVDEVRNFQQAGLIAYLDDDHFLRLSHVAIWNTRQTEFGKEMPFADGTSWGGMA
                     VGTPAPVTWLRLSHRIDPANGEHEFRAASSRDGRHWTWGGVWTLPAGTDPRIGLISHG
                     GAGAVADFDHFRIYRP"
BASE COUNT          486 a         1441 c         1247 g          457 t
ORIGIN      
        1 tcaccgacga cggagccgac cggacggccc tggcggcgct ggccgacgcc gggctcggcg
       61 tgcggctcgt cgacgcgcgg cggcagccgg ccgaggaggt ggcgtcgtga agctggtcaa
      121 gacctcgatc cggctggccg acgggcgcga gctgctctac ttcgacgagc gcgagggggt
      181 cgaccgccac gtccccgaca cccgcgacct caccgcggcc gtgacctcca gccagatccg
      241 ctgcgacccg ctgaccgagg agtgggtggt ggtcgcctcc caccgccagg gccggaccca
      301 cctgcccccg gccgaccagt gcccgctctg cccgtccagg gagggccggg cgaccgagat
      361 cccggccgcc gactacgacg tggtggtgtt cgagaaccgc ttcccgtcgc tggctcccga
      421 ccccggtccg gtggccgatg gcggaccgtt ccaccgccag ccgggggttg ggcgctgcga
      481 ggtcgtctgc ttcaccgccg accacggcgg ggcgctggcc cggctgccgg tcggccgcat
      541 ccgcacggtc gtcgaggtct gggccgaccg gaccctggag ctgggcgccc tggacggggt
      601 ggagcaggtg ttcccgttcg agaaccgggg cgaggagatc ggggtcaccc tgcaccaccc
      661 ccacgggcag atctacgcct acccgttcgt ggccccgcgg acggcccgcc agctggacgt
      721 ggcccgggcc taccgggacg ccaccggcga gtgccggagc tgttcgatcg tggccgccga
      781 ggccgacggg gagcgggtgg tggcgacggc gccgggctgg ctggccttcg tgcccttcgg
      841 cgcccgctgg ccgttcgagg tccacctgtt cccgacccgg cacctgcccg acctgccggc
      901 gctcggcgac gacgagcgcg acggcctggc ggcgctgctc aaggacgtcc tcggccgctt
      961 cgacgcgctg ttcgaccagc ccctgcccta catgctgacc tgccaccagg cgccggtgcg
     1021 gcgcgaccgc gacctggccc acgcccatct ggaggcgttc tccatccgcc gcacggccac
     1081 caagctcaag tacctggccg ccagcgagtc gggggcgggc gtgttcatca acgacatcgc
     1141 ccccgagcag gcggccgagc tcctgcgcgc cgccatgccc cattcccgct gaaccgggtt
     1201 ccggcgctga ttcgggtggt atacggccaa ctagactctt ctcacgaagt cgaacataaa
     1261 gtagcctgaa gcgaacagag acggtcaggg acgtccagcg aaggagtccg gtcccatgat
     1321 ccgtgccgtg gtctcaccgg gcggccgtca gcggccaggt cgaacgaccc tcgccgccgg
     1381 cctactgctc gccctctgcc tggccgggat cgccctcgca ccccgggccg gcgccgcccc
     1441 cagccccgcc acctacacga accccgtctc ccgcgacttc gccgacacct tcgccgaccc
     1501 ggccgtgatc aaggccaagg acggctggtg gtacgcctac gggaccaccg acccgctgcg
     1561 ggagggggag cgcacccggc acctcatccc cacggcccgc tcggccgacc tggtcaactg
     1621 gacctacgtc ggcgacgcct tcaccgagtc gacgctgccc gcctgggccg ccccggacgc
     1681 cgccctctgg gctcccgaca tccgctacct ggacggcacc tactacctct actacgtcgt
     1741 cacccagacg accgtgaccg acgagcccaa cgacaacgcc atcggcgtgg ccacggcccc
     1801 gaccccgacc gggccctgga ccgactcggg cgagccggtg gtcggccccc gccggggcga
     1861 cagcggcaac ccgggcgact tcaagtggac cttcgacccc tcgcagttca ccgacatcga
     1921 cggcacccgc tacctctact acggctccta ctacggcggc atcttcgtca ccgagctgaa
     1981 cgaggacgcc acccgggtgg tcggcacccc gaccatggtc gccatcgaca accgctacga
     2041 gggcgcctac gtcgtccgcc acggccgctg gtactacctg ttcgcctccg aggccaactg
     2101 ctgcgccggc ccgaccaccg gctacacggt gttcgcgggc cgctcagcca gccccctggg
     2161 accgttcacc gaccgcgacg gcatctcgct gctgacctcg cgggtcggcg ggaccatcgt
     2221 ggtcaccccc aacggcaaca cctgggtcgg caccggccac aacgccgtcg tcaccgacct
     2281 ggccggccag gactggttcg tctaccacgc catcgaccgg cgcgacccct tcctcgacga
     2341 gccgttcggc atcaacgagc ggccgatgct gctcgaccgc ctcgactgga tcgacggctg
     2401 gccgacggtc cgggccggcc gctgggcctc ggaggggccc cagcgggccc cggtcaccga
     2461 ctggacggtc gcgcttcccg acggcgctct tgacccgccg gccgacagct gcgagccgac
     2521 cttcgaggtc accgccggcc gctcgccgga cgaccaccgg gccgaggggg acctgcgcct
     2581 cggcgagggc gcgaccgccg ccgggctggt cacgagctgg cgcagccacc gcaactacgt
     2641 ggtcagctgg ctcgaccggg aggccggcgc cctggtcacc gacgtcgtcc ggggcgggag
     2701 gtcgctgggc cgtgaggcga gcccgctgcc ggccggcttc cgccacgaca cctggcacaa
     2761 cgtcgccgcc gagctgcgcg gccgtgtgct caccgtggag gtcaccgacg cccggctgca
     2821 cgacccggtg gccgtccagc gccgggtcct gccgcacggg accggcgggg ccggcgccgt
     2881 cggggcggcc gcccgctgcg ggccggccga ggcggccaac ctgggcgccg cccgcctgta
     2941 cgacccggtg cgccaccggg tgccgccacc gcgggtcggc gagctggtgt acgccgacga
     3001 gttcggcgac ggcaccgtgg gcggcccggg ctggagctgg gtgcgcgggc cggacccggc
     3061 cgtcaccgag accggtgggg cgctccgctg gccgacccag cccggcgacc tggtcggccc
     3121 cgacgagacc gcctcggtgc tgctgcgcga cgccccggcc ggcgcctaca ccgtcgagac
     3181 caggctggcc atcgacctcg gcgtcgacga ggtccgcaac ttccagcagg ccggcctgat
     3241 cgcctacctg gacgacgacc acttcctgcg cctgtcgcat gtggccatct ggaacacccg
     3301 ccagaccgag ttcggcaagg agatgccgtt cgccgacggc acctcctggg gcggcatggc
     3361 cgtcggcacc ccggccccgg tgacctggct gcgcctcagc caccgcatcg acccggccaa
     3421 cggcgagcac gagttccggg ccgcctccag ccgcgacggc cggcactgga cctggggcgg
     3481 ggtctggacg ctgcccgccg gcaccgaccc ccggatcggg ctgatctccc acggcggcgc
     3541 cggggccgtc gccgacttcg accacttccg catctaccga ccgtaggacc gttgccccgg
     3601 cggcctccgg ggcgagaaag gtggggacat g
//
LOCUS       DASSWR010000490         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_188194,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000490 DASSWR010000000
VERSION     DASSWR010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188194"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..3413
                     /locus_tag="VFO47_08195"
     CDS             57..3413
                     /locus_tag="VFO47_08195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET8788715.1"
                     /translation="MSATLTGVVTPGVRAALRGQDPTPEQLAAITAPIGPVHVIAGAG
                     SGKTAVMAARIVYLVERLGVAPASVLGLTFTNKAAGELETRVREALADIPVDAGDEVT
                     VDTYHAFAADLVKAYGIRVGVEVDADLLSEAQQYQILLGILDSERFEHLSVRTAGTTI
                     RKTLELASACADHVVPAERVVAASRRLLQRADEGERLPDWMLQAAKERIELARLVERY
                     AAEKRRRGRLDFGDQVAKAVELVEGHPELLDELRTRFQHVLLDEYQDTNVAQRRLMQR
                     ICPPGASIMAVGDARQAIYAFRGATMYNLLSFADHFPPTPATADPAVPGLAPVPAAPS
                     SGPGPVPVPAPGGGQDPASPAGAPLPLSTNFRSGPRILALANSVIDRIPEERRGGTSL
                     VARPGAAEGEVRAALLSDQFAEAAFVADQVVQAHETGLPDGSWPEWRDVAVLVRSKRL
                     LGPLREALEQRGVPVEVVGLSGLLETPEIVDLVSTLRVVADPGANVALARLLLGPRWR
                     IGPRHLVRLARWASRHNWGLKDELPGEDPDPGDVSFALAEALDHLDEVEGLDEEARHR
                     LGAFCEELRELRAAARGPLLDLVQTILERTGVWAELEASRDRRAITARQNLATFLDRV
                     AAFAPVQGDPSLGAFLVYLDAVEDASEPVEAVQPAPADSVKLMTVHMAKGLEFPMVAV
                     AGLSAGTGKDGSPRYGIFPDARVADPRRAQGFPYELREDAGHLPRFGGNARAFRTELE
                     ERALEDERRLFYVAITRAKQLLVLTSAWWYQGSEKTPKGPGPFWREAAGHPAVDVLVQ
                     AEKPVASPLTERLRERVSWPHPGRRPEDADDPVFPEGLAAAVELERAVPGTLESRVPP
                     GQADAFRAAAEAGWHLLDAARVDPTPARVEPPRILSVSQVLDYARCPRDFYWSVVRPL
                     PTAPKPAARLGTVIHRLLEERARSLPDLMDTDDLAGDQAGSHADPELIERARRNFAAT
                     RYATLPPPDAEVGVILRIGPWVVRGRVDAIFRSDGTGPAEVELVDWKTGRRVDQAVGG
                     LDQLALYALALRELGELPGDRCVVSYCYLGGDEPVLETRALGPADLDRQRALVEIILA
                     TLDQGDYQRACGRSDCETCRRGLGPPPRPP"
BASE COUNT          469 a         1479 c         1344 g          462 t
ORIGIN      
        1 gtgcccggtg gcgcgccggc ggccgggtcg gcggtgcgga acggcagggg agaacgatga
       61 gcgccaccct caccggtgtg gtcacccccg gggtccgggc ggcgctgcgg ggccaggacc
      121 ccacccccga gcagctcgcc gcgatcaccg ccccgatcgg cccggtgcac gtcatcgccg
      181 gcgccgggtc gggcaagacc gccgtgatgg cggcccgcat cgtctacctg gtggagcggc
      241 tcggggtcgc gccggcgtcg gtcctgggcc tgaccttcac caacaaggcc gccggcgagc
      301 tggagacaag ggtccgggag gcgctcgccg acatcccggt cgacgccggc gacgaggtca
      361 ccgtcgacac ctaccacgcg ttcgcggccg accttgtcaa ggcgtacggg atccgggtgg
      421 gggtcgaggt ggacgccgac ctcctcagcg aggcccagca gtaccagatc ctgctcggga
      481 tcctcgacag cgaacggttc gagcacctca gcgtccgcac cgccggcacc accatccgca
      541 agaccctcga gctggcctcg gcctgcgccg accacgtcgt ccccgccgag cgggtcgtgg
      601 cggcgtcccg ccgcctcctc cagcgggccg acgagggcga gcggctgccc gactggatgc
      661 tccaggcggc caaggagcgg atcgagcttg cccgcctggt cgagcgctac gcggccgaga
      721 agcggcggcg gggccgcctc gacttcggag accaggtggc caaggcggtc gagctggtgg
      781 aaggccatcc ggaactgctc gacgagctcc gcacccggtt ccagcacgtc ctgctcgacg
      841 agtaccagga caccaacgtc gcccagcgcc ggctcatgca gcggatctgc ccgcccggcg
      901 cctccatcat ggctgtcggc gacgcccgcc aggcgatcta cgccttccgc ggcgccacca
      961 tgtacaacct cctgtcgttc gccgaccact tcccacccac cccggcaacc gcggacccgg
     1021 cggtccctgg cctcgcgccg gtcccggccg ctccctcgtc cggacctggg cccgtccccg
     1081 tacccgcgcc aggtggcggt caggacccgg catccccggc cggggcgccg ttgccgctgt
     1141 cgaccaactt ccggtcgggg ccgcggatcc tggccctggc caactcggtg atcgaccgga
     1201 tcccggagga gcggcgcggg gggacctcgc tggtggccag gccgggcgcg gccgagggcg
     1261 aggtgcgggc ggccctgctc tcggaccagt tcgccgaggc cgcgttcgtg gccgaccagg
     1321 tcgtgcaggc ccacgagacc ggcctgcccg acggcagctg gcccgagtgg cgcgacgtcg
     1381 ccgtcctcgt ccggtccaag cggctcctcg gccccctgcg ggaggcgctc gagcagcggg
     1441 gcgtcccggt cgaggtcgtc ggcctgtccg gtctgctgga gacccccgag atcgtcgacc
     1501 tcgtctccac cctgcgcgtg gtcgccgacc cgggcgccaa cgtcgccctg gcccggctcc
     1561 tcctcggccc ccgctggcgg atcggccccc gccacctcgt ccggctggcc cgctgggcgt
     1621 cgcgccacaa ctggggcctc aaggacgagc tgcccggcga ggaccccgac ccgggcgacg
     1681 tcagcttcgc cctggccgag gcgctcgacc acctggacga ggtcgagggc ctcgacgagg
     1741 aggcgcgcca ccgcctgggc gcgttctgcg aggaactgcg ggagctgcgc gcggccgccc
     1801 gcgggcccct gctcgacctg gtccagacca tcctggagcg caccggcgtg tgggccgagc
     1861 tggaggcgtc ccgggaccgc cgggccatca ccgcccgcca gaacctggcc accttcctcg
     1921 accgggtggc ggcgttcgcc ccggtccagg gcgacccgtc cctgggcgcc ttcctcgtct
     1981 acctggacgc ggtcgaggac gcctccgagc cggtcgaggc ggtgcagccg gccccggccg
     2041 actcggtcaa gctgatgacc gtgcacatgg ccaagggcct ggagttcccg atggtcgcgg
     2101 tggccggcct ctcggccggc accggcaagg acggctcgcc ccggtacggg atcttccccg
     2161 acgccagggt cgccgacccc cgccgcgccc agggcttccc ctacgagctg cgtgaggacg
     2221 ccggccacct cccccgcttc ggcggcaacg cgcgggcgtt ccggaccgag ctggaggagc
     2281 gggccctcga ggacgagcgg cggctgttct acgtcgccat cacccgggcc aagcagctgc
     2341 tcgtgctgac ctcggcctgg tggtaccagg gctcggagaa gaccccgaag ggcccagggc
     2401 cgttctggcg cgaagccgcc ggccatccgg ccgtggacgt cctcgtccag gccgagaagc
     2461 cggtcgccag cccgctcacc gaacggctca gggagcgggt cagctggccc catccggggc
     2521 gccggcccga ggacgccgac gacccggtgt tccccgaggg cctcgccgcc gcggtggagc
     2581 tggaacgcgc cgtgccgggg acgctcgagt ccagggtgcc gccgggccag gccgacgcct
     2641 tccgggccgc cgccgaagcc gggtggcacc tgctcgacgc ggcccgggtc gacccgacac
     2701 cggccagggt cgagccgccc cggatcctct cggtcagcca ggtgctcgac tatgcccgct
     2761 gcccgcggga cttctactgg tcggtggtcc gtccgctgcc gacggcgccc aagcccgccg
     2821 ccaggctcgg caccgtgatc caccgcctgc tggaggagcg agcccgcagc ctccccgacc
     2881 tgatggacac cgacgacctc gccggcgacc aggcaggcag ccacgccgat cctgagctca
     2941 tcgaacgggc ccggcggaac ttcgcggcca cccgctacgc caccctccct ccacccgacg
     3001 ccgaggtcgg ggtgatcctc cgcatcggcc catgggtggt ccgagggcgc gtcgacgcca
     3061 tcttccgatc ggacgggacc gggcccgccg aggtcgagct ggtggactgg aagaccggcc
     3121 ggcgggtcga ccaggccgtg ggcgggctgg accagctcgc cctctacgcc ctcgccctgc
     3181 gtgagctcgg ggagctgccg ggggaccgct gcgtggtctc ctactgctac ctgggcggag
     3241 acgagccggt cctggagaca cgcgccctcg gcccggccga cctcgaccgg cagcgggcgc
     3301 tggtggagat catcctcgcc accctcgacc agggcgacta ccagcgggcc tgcggccgct
     3361 ccgactgcga gacctgccgc cgcggcctcg gccccccgcc ccgcccgcca tgaccggtgg
     3421 cggcgccgag cacctgcgcg aacggctcag ccgggcgctg ccggtggcca tgaaggcccg
     3481 ggatcgcatg gccgttgccg ccctgcggtc ggcattggcc gcgatcgcca acgccgaagc
     3541 cgtcgacccg tcccgggcgt caccgccggc cgccccgtcc ccgggccctg gtccgtctgc
     3601 ggagtcgccc ggcccgcgca ccgagcacca ggcacctgcc agttccggcc cggggcctcc
     3661 tgatccgcga accgggcacc aggaacctgc cgggccgggg tttgacccac cgttcgcggg
     3721 gacggtggcg gggattggcg cgaccgagat ggag
//
LOCUS       DASSWR010000491         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_556064,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000491 DASSWR010000000
VERSION     DASSWR010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556064"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFO47_08200"
     CDS             complement(<1..412)
                     /locus_tag="VFO47_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET8788716.1"
                     /translation="MRKRVAVVALAAALVFGMTLLAVPAAASGSSGKATTLAATLKGA
                     NEFPGPGDPDGRGRAFVRLAGDSACFVLQWSKITAPTAAHIHKGGPGVAGPVVVLFFQ
                     PGTNAASLPGTLDSVAGCVDVDPAVARKIAASPRD"
     gene            517..1284
                     /locus_tag="VFO47_08205"
     CDS             517..1284
                     /locus_tag="VFO47_08205"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET8788717.1"
                     /translation="MKIGIHLGRNDPLGEGAARGADCVQFFLGDPQGWKVPPPGPDAA
                     ELAAADMVVYVHAPYGINLASPNNRIRIPSRKLLADTCKGAAAVGAAGVIVHGGHVTG
                     GEDEAEGFPRWRKALDQLESEVPVLLENTAGGDHAMARRLGTLARLWDVVGDTGLGFC
                     LDTCHAHAGGEELDGIVDRVKAITGRIDLVHANDSKDRADSGRDRHENLGKGQIDLDT
                     LVGIVQAAGAPVICETPGGVAEHRADIELLRSRLGGR"
     gene            1319..1543
                     /locus_tag="VFO47_08210"
     CDS             1319..1543
                     /locus_tag="VFO47_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788718.1"
                     /translation="MPATPTVNPTRAALEAAVLDLTDEQIASALCKVDRAVKAAYEQR
                     AEYAWPREAQLSLLGALAQRLERGRVAAKG"
     gene            complement(1562..1813)
                     /locus_tag="VFO47_08215"
     CDS             complement(1562..1813)
                     /locus_tag="VFO47_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788719.1"
                     /translation="MRGDKVEVLVDTGNGGVRGFEIAATRSGRRVEVTTLRNMVEVAE
                     VTRTGQPVRTARFLSSRVVAIVEHPYDGGRPAAGTAGAG"
     gene            complement(1855..>2145)
                     /locus_tag="VFO47_08220"
     CDS             complement(1855..>2145)
                     /locus_tag="VFO47_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788720.1"
                     /translation="ELKEAAERLSTWRQACGRSGASSSPVGYPPEWGPTAGGSGGVTP
                     APPPAEVDAVLAADLDTPGALAVGDELAERGDGPGVAATAAVLGVELSPAGG"
BASE COUNT          295 a          811 c          796 g          243 t
ORIGIN      
        1 agtcgcgcgg gctggcggcg atcttcctgg ccacggcggg gtcgacgtcg acgcagccgg
       61 cgaccgagtc gagcgtgccg ggcagggagg cggcgttggt gccgggctgg aagaacagca
      121 ccaccaccgg gccggccacc cccggcccgc ccttgtggat gtgggcggcg gtgggggcgg
      181 tgatcttgga ccactggagc acgaagcagg cgctgtcgcc ggccagccgg acgaaggcgc
      241 ggccgcgccc gtccgggtcg cctgggcctg ggaactcgtt ggcccccttc agggtggcgg
      301 cgagagttgt ggccttaccc gagctgccgg aggcggcggc ggggacggcc agcaacgtca
      361 tgccgaagac gagggcggcg gccagggcga cgacggcgac acgctttcgc atgctcctgc
      421 tcctcgcgtc tcgggtgtct cgggagaatc gggggacaca gggacaacca cgggagggcc
      481 cgtccgcttc ccgtgctaag gtcgaaaatc gtggagatga agatcgggat ccatctgggg
      541 cggaacgacc cgctcggcga gggtgccgcc cggggggccg actgcgtcca gttcttcctc
      601 ggcgaccccc agggctggaa ggtgccgccg ccgggcccgg acgcggctga gctggccgcc
      661 gccgacatgg tcgtgtacgt ccacgccccc tacggcatca acctggcctc gcccaacaac
      721 cgcatccgga tcccgtcgcg caagctgctg gccgacacct gcaagggcgc ggccgcggtc
      781 ggcgcggccg gggtgatcgt gcacggcggc cacgtcaccg gcggcgagga cgaggccgag
      841 gggttccccc gctggcgcaa ggcccttgac cagctcgagt ccgaggtgcc ggtcctgctc
      901 gagaacaccg ccggcggcga ccacgccatg gcccgccgcc tcggcaccct ggcccggctg
      961 tgggacgtgg tcggcgacac cgggctgggg ttctgcctgg acacctgcca cgcccacgcc
     1021 ggcggcgagg agctggacgg catcgtcgac cgggtcaagg cgatcaccgg ccggatcgac
     1081 ctggtccacg ccaacgactc caaggaccgg gccgactcgg gccgcgaccg ccacgagaac
     1141 ctgggcaagg gccagatcga cctcgacacc ctggtcggga tcgtccaggc ggccggcgcc
     1201 ccggtgatct gcgagacccc gggcggcgtg gccgagcacc gggccgacat cgagctgctc
     1261 cgttcgcgcc tgggcggccg ctaagctacg cccacgccac cgagccggag gacgaccgat
     1321 gccagccacg ccgaccgtga acccgaccag ggccgccctg gaggccgccg tcctcgacct
     1381 caccgacgag cagatcgcca gcgccctctg caaggtcgac cgggcggtga aggcggccta
     1441 cgagcagcgc gccgagtacg cctggccgcg ggaggcgcag ctcagcctgc tcggggccct
     1501 ggcccagcgg ctggagcgcg gccgggtggc cgccaagggc tgacggcccg gcccgggccg
     1561 gtcagccggc cccggccgtt cccgccgccg gccggccgcc gtcgtagggg tgctcgacga
     1621 tggcgaccac gcgcgaggac aggaaccggg cggtgcgcac cggctggccg gtgcgggtca
     1681 cctcggccac ctcgaccatg ttccgcaggg ttgtcacctc gacccgccgc ccggaccgcg
     1741 tggccgcgat ctcgaacccg cggacgccac cgttgccggt gtcgaccagc acctcgacct
     1801 tgtccccgcg cataccgggc agcctagccg ctcccggccg aactggatag cccgtcagcc
     1861 gccagctggg gacagctcga ccccgagcac ggccgcggtg gccgccaccc cgggcccgtc
     1921 gccccgctcg gccagctcgt cccccaccgc cagcgccccc ggcgtgtcca ggtcggccgc
     1981 gagcaccgca tccacctccg ccggcggcgg agcgggcgtg acgccacccg accctccggc
     2041 ggtggggccc cactcgggag ggtagccgac cggggagctg gacgcgccgg accgtccaca
     2101 ggcctggcgc caggtggaga ggcgctcggc ggcctccttc agctc
//
LOCUS       DASSWR010000492         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_160389,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000492 DASSWR010000000
VERSION     DASSWR010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160389"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="VFO47_08225"
     CDS             <1..1943
                     /locus_tag="VFO47_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HET8788721.1"
                     /translation="YRMTFLAVALGMTFLVYRPWTRRRAGGAPAGEPGPDERPERPGN
                     GQPDNPGVLDWALAAAAVVVIAWPLLDFDAFVRRAVRPTTTDIALGLAAIALVLEATR
                     RTIGWILPAICLTFLAYAYYGNLIPVGYLLGHVGYDVDRLVGQTFMGVEGIFGVPLDV
                     AATYIVLFTIYGAVLEYSGAGRYFIDVSFAAFGRSAAAPARTVTLAGFLLGTVSGSGV
                     ATTVTLGSVAWPVLRRAGYPRDQGGGILAASGIGAILSPPTLGAAAFIIAEFLNVSYL
                     QVLLFATIPTVLYYLGIVLAIEADSRRFRTRAVELDTPPLGRLLLRWGYHFSSLFLIV
                     ILMALGLSPFRAVLYATVAAFLLSFLDRSTWMTPRRTWEALAAGAIGVLPVAATTAAA
                     GIIVAVVTLTGLGLKVSSIIVDLAGGRLGLAAFYSAIAVLVLGLAVPVTASFIIAAVI
                     IGPALTTLGVEPFAAYMFIFYYAVLSEVSPPTALSAVAAAAITGGNPFRTMMLTWRYT
                     LPAFLVPFAFVLSPRGEALLLEGPVGTILLALAVSVVAVAALALATGAWLAGPACWPE
                     RVLAGFAAVLLLYLEPAAIGAGLALAVAAVAVHLLTRRRRAAGGAAPAGEAGRPVGED
                     AKPRGPDETDETDETTEARRQP"
     gene            1940..2914
                     /locus_tag="VFO47_08230"
     CDS             1940..2914
                     /locus_tag="VFO47_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET8788722.1"
                     /translation="MRGWRRWAVPTLVALVALAGCGGGGGGGGGGGADSRRLSIATGG
                     TGGVYFVYGGGLAKLITSSLEGYEATAEVTSASVDNMTLIADEKSALAFTLADTASDA
                     VQGRGSFKEQVPAQALAQLYTNYTQVVATAGKGIERIEDLKGKRVSVGSPNSGTEVIA
                     LRILEEAGIDPEADLRRQQLGVAESVQAVKDGSLDAFFWSGGLPTGAVTDLATSRRIV
                     LLPTDEYVQPLRSQYGEVYAETTIPGGTYKGVADGVKVIGVPNYLVVNRSMSEDLGYQ
                     LTKLLFDKKPELVKVHPEAKNLDLETAQQVTPLELHPGAQRYYQETAG"
     gene            2925..3434
                     /locus_tag="VFO47_08235"
     CDS             2925..3434
                     /locus_tag="VFO47_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788723.1"
                     /translation="MAAGRRRWPIAVAVPLALAAAAGLLPQRAVVTVTGADGRPVAAR
                     VLPAPGRFELRYRHSYYRVEAAESFVAAPDGGFRLVAVGSPSQAVLDYYGLTGRTHRE
                     GGWLRLELRQPQRFERLPLVATGLGRRTLVVGGQAVPLAPPGGEPAHLMIAIERRPWL
                     LTLRAGPGP"
     gene            complement(3593..3934)
                     /locus_tag="VFO47_08240"
     CDS             complement(3593..3934)
                     /locus_tag="VFO47_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788724.1"
                     /translation="MSVRWYERLLGAKPVLDEDAGGFHHTVYLLPGGALLGLHQHPAT
                     NRDDRFDEHKPGLDHLSFGVTDRAELKAWQTRLEELGIPHGEIVDAHYGSGLSFRDPD
                     NIALELFTPPG"
     gene            3953..>4159
                     /locus_tag="VFO47_08245"
     CDS             3953..>4159
                     /locus_tag="VFO47_08245"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET8788725.1"
                     /translation="MAMLAPLISEMLGVQTAPIEYVEDGRRHRVRIGDEVEIEIEDFV
                     PPQTPEGEVSKLTGMFHPANSTLTI"
BASE COUNT          525 a         1476 c         1494 g          664 t
ORIGIN      
        1 ggtaccggat gaccttcctg gccgtcgccc tgggtatgac gttcttggtc taccggccct
       61 ggacgcgccg gcgggcggga ggggcaccgg ccggcgaacc cgggccggac gagcggcccg
      121 agcgacccgg gaacgggcag cccgacaatc cgggggtgct cgactgggcg ctggcggccg
      181 cagccgtcgt cgtcatcgcc tggccactgc tcgacttcga tgcgttcgtg cgccgtgccg
      241 tgcggccgac caccaccgac atcgccctgg gtctggccgc catcgcgctg gtgctggagg
      301 cgacccgccg taccatcggc tggatcctgc ccgccatctg cctcaccttc ctggcctacg
      361 cctactacgg caacctcatc cccgtcgggt acctgctcgg ccacgtcggc tacgacgtgg
      421 accggctggt cggccagacg ttcatgggcg tggagggcat cttcggcgtg ccgctggacg
      481 tggccgccac ctacatcgtg ctgttcacca tctacggggc ggtgctggag tactcaggcg
      541 ccggccgcta cttcatcgac gtgagcttcg ccgccttcgg caggtcagcg gctgcgccgg
      601 cccggaccgt gaccctcgcc gggttcctgc tcgggacggt gtcagggtcg ggggtggcga
      661 ccacggtcac gctcggctcg gtggcctggc cggtgctgcg ccgggccggc tatccgcgcg
      721 accagggcgg tgggatcctg gccgcttccg gcatcggcgc gatcctatcg ccgccgaccc
      781 tgggcgcggc cgcgttcatc atcgccgagt tcctgaacgt cagctacctc caggtgctgc
      841 tgttcgccac catccccacc gtcctgtact acctgggcat cgtgctggcc atcgaggccg
      901 actcccgccg cttccgcacc cgggcggtgg agctggacac gccgccgctc ggtcgcctgc
      961 tgctgcgctg gggctatcac ttcagctcgt tgttcctcat cgtgatcctg atggcgctcg
     1021 gcctgtcacc gttccgggcg gtgctgtacg ccaccgtcgc cgccttcctg ctgtcgttcc
     1081 tggaccgctc gacctggatg acccccaggc gcacgtggga ggcgctggcc gctggcgcca
     1141 tcggggtgct gccggtggcg gccacgaccg cggccgccgg gatcatcgtc gccgtcgtca
     1201 ccctgaccgg gctcgggctg aaggtcagct cgatcatcgt cgacctggct ggcggccggc
     1261 tggggctcgc cgctttctac tcggccatcg ccgttctcgt cctcgggttg gccgtgccgg
     1321 tgaccgcttc gttcatcatc gccgcggtga tcattggccc ggccctgacc accctggggg
     1381 tggagccgtt cgcggcctac atgttcatct tctactacgc ggttctctcc gaggtgagcc
     1441 cgccgaccgc gctgtcggcg gtggccgcgg ccgccatcac cggcggcaac ccgttccgca
     1501 ccatgatgct cacctggcgg tacacgctgc cggcgtttct tgtcccgttc gcgttcgtgc
     1561 tgtcaccgcg cggcgaggcg ctgctgctgg aggggccggt gggcaccatc ctgctggcgc
     1621 tggccgtgtc ggtggtcgcg gtggccgcgc tggcgctggc caccggcgcc tggctggccg
     1681 gtccggcctg ctggcccgag cgggtcctgg ccggctttgc cgctgtgctc ctgctgtacc
     1741 tggagccggc ggcgatcggc gccgggctcg ccctggccgt ggccgccgtc gccgtccacc
     1801 tgctcacccg ccgacggcgg gcggccggcg gtgcggcacc ggccggggag gcgggccggc
     1861 cggttgggga ggacgccaag cccaggggac ctgacgagac cgacgagact gatgagacca
     1921 ccgaggcaag gaggcaacca tgagaggctg gcggcggtgg gccgtgccca ccctggtcgc
     1981 gctggtggcc ctggccggct gcggcggcgg cgggggagga ggcggaggcg gcggcgccga
     2041 ctcgcggagg ctgtcgatcg ccaccggagg gaccggcggc gtctacttcg tctacggcgg
     2101 cgggctggcc aagctgatca cctcgtcgct ggagggctac gaggcgaccg ccgaggtcac
     2161 ctccgcctcc gtcgacaaca tgacgctgat cgccgacgag aagagcgcgc tcgccttcac
     2221 cctggccgac acggcgagcg acgccgtgca gggccgcggc tcgttcaagg agcaggtacc
     2281 agcccaggcc ctggcccagc tgtacaccaa ctacacccag gtggtcgcca ccgccggcaa
     2341 gggcatcgag cgcatcgagg acctcaaggg caagcgggtg tcggtcgggt cgcccaattc
     2401 aggcaccgag gtgatcgccc tgcgcatcct ggaggaggcc gggatcgacc ccgaggccga
     2461 ccttcgccgc cagcagctcg gcgtggccga gtcggtccag gcggtcaagg acggctcgct
     2521 ggacgccttc ttctggtcgg gtgggctgcc caccggcgcc gtcaccgacc tggccaccag
     2581 ccgccggatc gtgctgctgc caaccgacga gtacgtgcag ccgctgcgca gccagtacgg
     2641 cgaggtctac gccgagacga ccatccccgg cggcacctac aagggtgtcg ccgacggcgt
     2701 gaaggtgatc ggggtgccca actacctggt cgtcaaccgg tcgatgagcg aggaccttgg
     2761 ctaccagctc accaagctgc tgttcgacaa gaagcccgag ctggtcaagg tccacccgga
     2821 ggccaagaac ctggatctgg agaccgccca gcaggtcacg ccgctggagc tgcaccctgg
     2881 cgcgcagcgg tattaccagg agacggccgg ctaggccgcc ggcgatggcc gccgggcgcc
     2941 gccgctggcc gatcgcggtc gccgtgccac tggcgctggc cgccgccgcg ggactgctgc
     3001 cccagcgggc ggtggtcacg gtgaccggcg ccgatggccg gccagtggcg gcgcgggtcc
     3061 tgccggctcc cggccggttc gagctgcgct atcgccactc ctactaccgg gtcgaggcgg
     3121 ctgagtcgtt cgtggccgcc ccggacgggg gcttccgact ggttgcggtc ggctcgccga
     3181 gccaggccgt gctcgactac tacggcctga ccgggcggac gcaccgagag ggcggctggc
     3241 tgcggctgga gctgcggcag ccgcaacgct tcgagcggct gccgttggtg gcgactgggc
     3301 tcggccggcg cacccttgtc gtcggcggtc aagcggtacc gctggcgccc cccggcggag
     3361 aaccggccca cctgatgatc gccatagagc ggcgcccatg gctgctcacc ctgcgggccg
     3421 gccctggccc atgacccctc aacgcagccg ggaggtggat cgtaggcggc acgatcaatg
     3481 cggctgcgct gccatcgctg cggtcaccgg cagtacttcc tgccgccggg tggcgacagt
     3541 cctcggccac gggcccagct gatcggtctc ggcctgtggc cgtcgttctg acctatccgg
     3601 gcggtgtgaa gagttccagg gcgatgttgt cggggtcgcg gaaggacagc cccgagccgt
     3661 agtgggcgtc aacgatctct ccgtgtggaa tcccgagctc ctcgagccgg gtctgccacg
     3721 ccttgagctc ggcgcggtcg gtgaccccga aggagagatg gtccaggccc ggcttgtgct
     3781 cgtcgaagcg gtcgtctcgg ttggttgccg gatgctggtg gagccccagg agcgcgcctc
     3841 ccgggagtag gtacaccgtg tggtggaagc cgccggcgtc ctcatcgagc accggcttgg
     3901 cgccaagcag gcgctcatac cagcggacgc tcacggccag ctcggtggcc cgatggccat
     3961 gctggcgccg ctgatcagcg agatgctcgg ggtccagacc gccccgatcg agtacgtcga
     4021 ggatggccgg cgccatcggg tcaggatcgg cgacgaggtc gagatcgaga tcgaggactt
     4081 cgtgccgccg cagacccccg agggtgaggt gtccaagctc accggcatgt tccatccggc
     4141 caactcgacc ctgacgatc
//
LOCUS       DASSWR010000493         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1234233, whole genome shotgun sequence.
ACCESSION   DASSWR010000493 DASSWR010000000
VERSION     DASSWR010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234233"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFO47_08250"
     CDS             complement(<1..836)
                     /locus_tag="VFO47_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8788726.1"
                     /translation="MSQPLRYTLKTWGRRLLIFAIIYILFIPVLYWMYKSNRGLDWWN
                     ILGGDPRVGPEFRSYLIKNDHPLAPEFDLWATTRALPFAIISAIVTLALFVAIVLIQF
                     VAIFWFLARGQTYVIYPREYDVSFDDVRGQPEIVESTKEALTLFQGFKKFRDAGGYPP
                     HGILFEGPPGTGKTLLGKAIAGSANVPFVYASGTSFNNMFMGVGNLRIMRLFKKARKL
                     SRKYGGAVIFLDELDAVGGTRGAVSTASEPADTTRHWLAKGPLRIIMGGMGMGGAGMY
                     VN"
     gene            1003..2637
                     /locus_tag="VFO47_08255"
     CDS             1003..2637
                     /locus_tag="VFO47_08255"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET8788727.1"
                     /translation="MPGLAVIAHTVWGAAGPSLHDPRNRLGDALGRRLDLAVAVESGR
                     ITAVVPAAEVGALGADRVVDLGDATLMPGFVDAHVHLSATGLAGHGADLRQARSAAEV
                     LERVRAAAARDGADGLLWGDGYDETRFEVPELPTPAELATAAGGRPLYLSRVDGHQGL
                     ATLDVLGDSGALEAAGCDRDPAGAPTGVTRGEANHLARRFALATLPRATLLAAQDAAL
                     CQAARRGVTCVHEMGGPDIAGKRDFELLLDRVEALPIEVIGYWGDRDLDYVAERKLAQ
                     VGGDLFLDGSLGSHTAALSTPYEDRPDTSGALYHDDQELAELYVRASQAGIQVGVHAI
                     GDVAIGQALRCARRAMKVVGPTAFRGCRHRIEHVELLGADGADRMAELGLAASVQPAF
                     DAAWGGSDGMYARRLGPRRAKSMNPFADLWRRGVPTGGSSDSHVTPLDPWHGVAAAVH
                     HHRPSQRLGLPVALEVFTLGGRILARQERISGTIEPGQRADLTAFAGDVLAADPADLE
                     GGEAIFTMVAGRLAHGPDELHVEAARSFGGWTCTRR"
BASE COUNT          398 a         1081 c          991 g          376 t
ORIGIN      
        1 tcgttgacgt acatgccggc gccgcccatg cccatgccgc ccatgatgat ccgcagcggc
       61 cccttggcca gccagtggcg ggtggtgtcg gcgggctcgc tggcggtcga caccgccccg
      121 cgggtcccgc ccaccgcgtc cagctcgtcc aggaagatca cggccccgcc gtacttgcgc
      181 gacagcttcc gggccttctt gaacaggcgc atgatgcgca ggttgccaac gcccatgaac
      241 atgttgttga agctggtccc cgaggcgtac acgaacggca cgttggccga cccggcgatc
      301 gccttgccga gcagggtctt gcccgtgccc ggcgggccct cgaacaagat cccgtggggc
      361 gggtagccgc cggcgtcgcg gaacttcttg aacccctgga acagggtgag cgcttccttg
      421 gtcgactcga cgatctcggg ctggccgcgg acgtcgtcga agctgacgtc gtactcgcgc
      481 gggtagatga cgtaggtctg gccccgggcc aggaaccaga agatggcgac gaactggatc
      541 agcacgatgg ccacgaacag ggccagggtg acgatcgcgc tgatgatcgc gaacggcagg
      601 gccctggtcg tcgcccacag gtcgaactcg ggggccaggg ggtggtcgtt cttgatcagg
      661 tagctgcgga actcgggccc gacgcggggg tcaccgccca ggatgttcca ccagtccaga
      721 ccccggttgg acttgtacat ccagtacagc acggggatga acaggatgta gatgatggcg
      781 aagatcagca gccgccgacc ccatgtcttc agcgtgtagc gaagtggctg actcacggac
      841 ccgccttcct gacacgactc gccttgccga cctggtcacg ctgcctacga gtatgacccg
      901 cgacctctga taccgctata gcggggggtc accctctggt cgggcgtggg ggacagcgcc
      961 tagactgcca acctgacgat ccccgaggag gttccttcgc ccgtgcccgg cctcgcggtc
     1021 atcgcccaca ccgtctgggg cgcagccggg ccgagcctcc acgacccccg caaccgtctc
     1081 ggcgacgccc tcggccgccg cctcgacctg gccgtggccg tcgagagcgg gcgcatcacc
     1141 gccgtcgtcc cggccgccga ggtgggcgcc cttggcgcgg accgggtggt cgacctgggc
     1201 gacgccaccc tgatgcccgg gttcgtcgac gcccacgtgc acctgtcggc caccggcctg
     1261 gccggccacg gcgccgacct gcgccaggcc cgctcggcgg ccgaggtcct ggagcgggtc
     1321 cgggcggccg cggcccgcga cggcgccgac ggcctgctct ggggcgacgg ctacgacgag
     1381 acccgcttcg aggtccccga gctgcccacc ccggccgagc tggccacggc ggccggaggc
     1441 cgcccgctgt acctgtcgcg ggtcgacggc caccagggcc tggccaccct ggacgtgctc
     1501 ggcgactcgg gcgcgctgga ggcggccggc tgcgaccgcg accccgccgg cgcccccacc
     1561 ggggtgaccc ggggtgaggc caaccacctg gcccggcggt tcgccctggc caccctgccc
     1621 agggccaccc tgctggccgc ccaggacgcc gccctctgcc aggcggcccg gcggggcgtg
     1681 acctgtgtcc acgagatggg cgggcccgac atcgccggca agcgcgactt cgagctgctg
     1741 ctcgaccggg tcgaggccct gcccatcgag gtcatcggct actggggcga ccgcgacctg
     1801 gactacgtgg ccgagcgcaa gctggcccag gtgggcggcg acctgttcct ggacggatcg
     1861 ctcggctccc acaccgccgc cctgtccacc ccctacgagg accggcccga caccagcggg
     1921 gccctgtacc acgacgacca ggagctggcc gagctgtacg tgcgggccag ccaggccggg
     1981 atccaggtcg gcgtccacgc catcggggac gtggccatcg gccaggcgct gcgctgcgcc
     2041 cggcgggcca tgaaggtcgt cgggccgacc gccttccggg gctgccggca ccggatcgag
     2101 cacgtcgagc tgctcggcgc cgacggcgcc gaccgcatgg ccgagctggg gctggccgcc
     2161 agcgtgcagc cggccttcga cgcggcctgg ggcgggagcg acggcatgta cgcccgccgc
     2221 ctcggccccc ggcgggccaa gagcatgaac ccgttcgccg acctgtggcg gcgcggggtg
     2281 ccgaccggcg gctcctccga ctcccacgtc accccgctcg acccctggca cggggtggcc
     2341 gccgccgtcc accaccaccg gcccagccag cgcctcgggc tgcccgtggc ccttgaggtg
     2401 ttcaccctcg ggggcaggat cctggcccgc caggagcgga tcagcggcac catcgagccc
     2461 ggccagcggg ccgacctgac cgccttcgcc ggcgacgtgc tcgccgccga cccggccgac
     2521 ctggagggcg gcgaggcgat cttcaccatg gtcgccggcc gcctcgccca cgggcccgac
     2581 gagctccacg tcgaggccgc ccggagcttc ggcggctgga cctgcacacg caggtagact
     2641 cgtctccgaa tctcaccgca cggccccgcg tcctccatcg aaggagcccc catggccaag
     2701 ttccgagtca cctacgccac cctctcggcc gacaacgagg agctccacgc cgccttcgag
     2761 gagggcgtga agaccgccag gtcctggatc ggccagacca tccccacggt ggtcgacggc
     2821 gagccccgca ccgacgggga gccgtt
//
LOCUS       DASSWR010000494         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1234253, whole genome shotgun sequence.
ACCESSION   DASSWR010000494 DASSWR010000000
VERSION     DASSWR010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234253"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VFO47_08260"
     CDS             complement(<1..1926)
                     /locus_tag="VFO47_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8788728.1"
                     /translation="MPRLTRFLLRHKLAVVAAWLVVLVAGVAAAGVVPGRLSQEFSFP
                     GEEGYEANVAILEAYGNGGPGNPLVPVVTLPAGTTVDSPGARDALDRAFGGVAADPRL
                     RVLAWPATTGDRRLVVEDGRTVYGLVWGPFQGPEGADPALAGTLADGLRQALPAGTTV
                     QVTGLDALRTAAAEEPAGTGVLIETLAGALGALVVLAFVFGSFLALVPLLIAAVAILT
                     TFLAVLGLTGLVEVRFIVQFLVALIGLGVAVDYSLLVVTRWREELGRGLDREEAVQRA
                     MATAGRAVVTSGGTVAVGLLALVLLPVPFLRSIGYAGLLIPLAATLATLTLLPVLLAT
                     VGPRLDWPAGRRARRDPGRGIAAGPLWTRWATGVTRHRWLATLAALAVLLPLCVAALD
                     LRLGEPPAAVLAQSGPAREALDRLERAGVSSGVLTPIEVLVPAGADPEAAAGRLGGVA
                     GVQLAVAPAGPAWQRDDSGLVSVLPVAETSGDEGEATVERVRDLAAAELPGARVGGSG
                     ALAIDATASLYGSFPLMLAVVAAVTFVLLARAFRSLLLALKAILLNLLSIGAAYGVLV
                     LVWQEGYGSEAIWGIPATGAIAMWVPLMAFAFLYGLSMDYEVFLLARMREEYDATGST
                     QTAIVQGLGRVGRLVTCA"
     gene            complement(2066..>2641)
                     /locus_tag="VFO47_08265"
     CDS             complement(2066..>2641)
                     /locus_tag="VFO47_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788729.1"
                     /translation="GDGAAAVDLTRSTRPDVVLMDIRMPVLDGLEATRRIVADELLAG
                     VRVLVLTTFELDEYVFEALAAGASGFLLKNTDPVELLRAIRVVAEGEALLAPSVTRRL
                     IAEFTAARRQAAGRPAGVDLAALTDREREVMALAGAGLSNEEIAERLVVSPATARTHV
                     SRAMVKLGARDRAQLVVFAYESGLVSPGHLR"
BASE COUNT          359 a          998 c         1033 g          251 t
ORIGIN      
        1 ggcgcaggtg accaggcggc cgacccggcc caggccctgg acgatggccg tctgggtcga
       61 gccggtggcg tcgtactcct cgcgcatccg ggccagcagg aacacctcgt agtccatgga
      121 caggccgtac aggaacgcga aggccatcag cggcacccac atggcgatcg ccccggtggc
      181 cgggatgccc cagatcgcct ccgagccgta gccctcctgc cagaccagca ccagcacccc
      241 gtaggcggcg ccgatggaca gcaggttgag caggatcgcc ttcaaggcca gcagcagcga
      301 gcggaacgcc ctggccagga gcacgaaggt gacggcggcg acgacggcca gcatcagcgg
      361 gaagctgccg tagaggctgg ccgtggcgtc gatggccagc gcgccggagc cgccgaccct
      421 ggccccgggc agctcggccg cggccaggtc gcggacccgc tcgacggtcg cctcgccctc
      481 gtcgccgctc gtctcggcga cgggcaggac gctgaccagc cccgagtcgt cgcgctgcca
      541 ggccggcccg gcgggggcga cggccagctg cacgcccgcg accccgccca ggcggccggc
      601 ggccgcctcc gggtcggcgc cggccgggac cagcacctcg atcggggtga ggacgccgct
      661 ggagaccccg gcccgctcca ggcggtcgag ggcctcgcgg gccggtccgg actgggccag
      721 gacggccgcc ggcggctcgc ccaggcgcag gtccagggcg gccacgcaga gggggaggag
      781 cacggccagc gcggccaggg tggccagcca ccggtggcgg gtcaccccgg tcgcccagcg
      841 ggtccagagg gggccggccg cgatcccccg ccccgggtcg cggcgggccc ggcggccggc
      901 cggccagtcg agccggggcc cgacggtggc cagcagcacc ggcagcaggg tgagggtggc
      961 cagggtggcg gccagcggga tcagcaggcc ggcgtagccg atgctgcgca ggaacggcac
     1021 cggcagcagg accagggcca gcaggcccac ggcgacggtg ccgccgctgg tcacgaccgc
     1081 ccgcccggcg gtggccatgg cccgctggac ggcctcctcg cggtccaggc cccggcccag
     1141 ctcctcgcgc cagcgggtca cgaccagcag cgagtagtcg acggccaccc cgagcccgat
     1201 cagcgcgacc aggaactgga cgatgaatct gacctcgacc agcccggtga ggccgagcac
     1261 ggccaggaag gtggtgagga tggccacggc cgcgatcagc agcggcacca gggccaggaa
     1321 cgagccgaac acgaaggcca gcacgaccag ggcgccgagg gccccggcga gcgtctcgat
     1381 cagcacgccg gtgccggccg gctcctcggc cgcggccgtg cgcagcgcgt ccaggccggt
     1441 cacctgcacg gtcgtgccgg ccgggagggc ctggcgcagc ccgtcggcca gcgtcccggc
     1501 cagggccggg tcggcgccct cggggccctg gaacgggccc cagacgagcc cgtagacggt
     1561 gcggccgtcc tcgaccacca ggcggcggtc gccggtggtg gccggccagg ccagcacccg
     1621 cagccgcggg tcggcggcga ccccgccgaa ggcccggtcg agggcgtccc gggccccggg
     1681 gctgtcgacg gtggtccccg cggggagcgt gaccaccggg accagcgggt tgccggggcc
     1741 gccgttgccg taggcctcca ggatggccac gttggcctcg tagccctcct cgccggggaa
     1801 ggagaactcc tgggacagcc ggcccgggac cactccggcc gcggccaccc cggcgaccag
     1861 gacgaccagc caggccgcga ccacggccag cttgtggcgg agcaggaatc gggtcagtcg
     1921 cggcatggaa gcctccgggt cgctggtggg actgggcggc ccgatcgtgc cgcccggggg
     1981 ccgggccggt cgtcggccac agagcggctc cgcgcctgcg cagcccgcgc aggcggggcc
     2041 gcccggctac ggcggcgggc gtaggtcagc ggaggtggcc ggggctgacc aggccggact
     2101 cgtaggcgaa gacgaccagc tgggcgcggt cgcgggcgcc cagcttgacc atggcccggc
     2161 tcacgtgggt gcgggccgtt gccgggctca ccaccagccg ctcggcgatc tcctcgttcg
     2221 acagcccggc cccggccagg gccatgacct cccgctcgcg gtcggtcagg gcggccaggt
     2281 cgaccccggc cggccggccg gcggcctggc ggcgggcggc ggtgaattcg gcgatcagcc
     2341 ggcgggtcac gctcggggcc agcagggcct cgccctcggc cacgacccgg atcgcccgca
     2401 gcagctccac cgggtcggtg ttcttgagca ggaacccgct ggccccggcg gccagtgcct
     2461 cgaacacgta ctcgtccagc tcgaaggtgg tcaggaccag cacccggacc ccggccagca
     2521 gctcgtcggc gacgatgcgg cgggtggcct ccaggccgtc gagcacgggc atgcggatgt
     2581 ccatcagcac gacgtcgggc cgggtcgagc gggtcaggtc gaccgcggcc gccccgtccc
     2641 c
//
LOCUS       DASSWR010000495         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_922271,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000495 DASSWR010000000
VERSION     DASSWR010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922271"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VFO47_08270"
     CDS             complement(<1..171)
                     /locus_tag="VFO47_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET8788730.1"
                     /translation="MARVKRAVHGVKAKRTTMELAKGYRGSRGRHYRAANEQVMHALR
                     YAYRDRRARKGDF"
     gene            complement(215..409)
                     /gene="rpmI"
                     /locus_tag="VFO47_08275"
     CDS             complement(215..409)
                     /gene="rpmI"
                     /locus_tag="VFO47_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET8788731.1"
                     /translation="MPKMKSHRGATRRFRVTKTGKLVRRQTRLNHMLEKKTPGHKRRL
                     GREVEVAAGDRRSVRRMLGL"
     gene            complement(423..1034)
                     /gene="infC"
                     /locus_tag="VFO47_08280"
     CDS             complement(423..1034)
                     /gene="infC"
                     /locus_tag="VFO47_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET8788732.1"
                     /translation="MGGPCAAFFVWASAQRRDVSISVEPRINDRIRTPQVRVVGADGE
                     QLGVMDTQAALRMASQQDLDMVEVAPMARPPVVKLMDYSKFKYEQAIKAKEARRKQNL
                     VALKEMKMRPKIDPHDYQTKKGHVERFLKQGHKVKVTIMFRGREMAHTELGRRLLERL
                     ATDLVEIAQVDSPARQDGRNMITVFSPRKTQGGAKGQGPVRTP"
     gene            1223..1627
                     /locus_tag="VFO47_08285"
     CDS             1223..1627
                     /locus_tag="VFO47_08285"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET8788733.1"
                     /translation="MSEQDLTPDQLEQLRQQLASVAAGDVVAEAALSLIALAYVRLGI
                     PPEQHARFRDLDQARLLVDALGGLLSATEGRLGAPEAGLRDALANLRMAYAEVAAAAE
                     GSAPRTPPGTEPEGEDSGILRPPSGLWVPGQE"
     gene            complement(1728..1931)
                     /locus_tag="VFO47_08290"
     CDS             complement(1728..1931)
                     /locus_tag="VFO47_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET8788734.1"
                     /translation="MPEGTVKWFSNEKGYGFIARNGGDDVFVHFSAIQSDGYKTLNEG
                     QAVEFEITDGPKGPQASNVRPLA"
     gene            complement(2086..2961)
                     /gene="hisG"
                     /locus_tag="VFO47_08295"
     CDS             complement(2086..2961)
                     /gene="hisG"
                     /locus_tag="VFO47_08295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET8788735.1"
                     /translation="MLKLVLPKGSLERATLALFEAADLPVKRGSERDYHGAIDDPRVE
                     RVSLLRPQEIPQYVEEGFFDLGITGRDWIEETGSKVVSLAELPYSKQTSNPVRVVLAV
                     HRDLEVTRPEELQPGLRVSTEFPNLTRRFFAERGLPARIFPSYGATEAKVPEIVDAVV
                     DLTETGSTLRSHGMVVIATLLTSWTEVIANAESAADPERRQAMDDLLTLLVGAATARS
                     KVLIKLNVADADLHRVLAVLPSMKAPTVNELADSGFHAVETVVDKAGVNRLIPTLKAA
                     GAFDILELPISKIVA"
     gene            complement(2983..>3295)
                     /gene="ribH"
                     /locus_tag="VFO47_08300"
     CDS             complement(2983..>3295)
                     /gene="ribH"
                     /locus_tag="VFO47_08300"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET8788736.1"
                     /translation="GAFELPLAAERLAATGRYAAVVAIGAVVRGATPHFDHVAGQAAA
                     GLAAVTRASGVPVAFGVLTCDTMEQALDRAGGKAGNKGAEAAVCALEMAGLLEAIDKQ
                     D"
BASE COUNT          426 a         1145 c         1171 g          553 t
ORIGIN      
        1 gaagtcgccc ttgcgcgccc ggcggtcgcg gtaggcgtag cgcagggcgt gcatgacctg
       61 ctcgttggcg gcgcggtagt ggcgtccccg gctgccccgg tagcccttgg ccagctccat
      121 ggtggtgcgc ttggccttga cgccgtggac ggctcgcttg actctcgcca tcggagttct
      181 ctttccgttt cgtcggttac aggtgcagac cccgctacag gccgagcatg cggcgcacgc
      241 tgcgccggtc gccggcggcg acctcgacct cgcggcccag gcggcgcttg tggccggggg
      301 tcttcttctc gagcatgtgg ttcagccggg tctgccggcg gaccagcttg ccggtcttgg
      361 tcacccggaa gcgccgggtg gcgccccggt ggctcttcat cttgggcacg gggtctcctg
      421 ggttatggcg tgcgcacggg tccttgtccc ttggcgccgc cctgggtctt gcggggcgag
      481 aacaccgtga tcatgttgcg cccgtcctgg cgggccgggc tgtcgacctg ggcgatctcg
      541 acgaggtcgg tggccagccg ctccagcagc cgccgcccga gctcggtgtg ggccatctcg
      601 cggccgcgga acatgatggt gaccttgacc ttgtggccct gcttgaggaa ccgctcgacg
      661 tgacccttct tggtctggta gtcgtgcggg tcgatcttgg gccgcatctt catctctttg
      721 agggccacca ggttctgctt gcgcctggct tccttcgcct tgatggcctg ctcgtacttg
      781 aacttcgagt agtccatcag cttgaccacc ggcgggcggg ccatcggggc gacctcgacc
      841 atgtcgaggt cctgctggct ggccatccgc agcgcggcct gggtgtccat caccccgagc
      901 tgctcgccgt cggcgccgac cacccggacc tggggggtcc ggatccggtc gttgatcctg
      961 ggttctacgc tgatgctgac atccctcctc tgggccgatg cccaaacgaa aaaggcggcg
     1021 caagggccgc ccaccaattc ggaccggtga ggtcatggtg accccgtggg cgtttcgccc
     1081 agaggtgaga agccctcgct tctgctttgc tccgtatgca gttgacgggc ccgagggagc
     1141 gaacaggatg ttctcggacc cgaccgaatc aacgctactg tagcgtcgat tgttccagct
     1201 gtcgacgccg cgaggagccc cgatgtcgga gcaggacctg acccccgacc agctcgagca
     1261 gctgcgccag cagctcgcct cggtggcggc cggcgacgtg gtcgccgagg cggccctctc
     1321 gctgatcgcg ctggcctatg tccgcctcgg catcccgccc gagcagcacg cccgcttccg
     1381 cgacctggac caggcccggc tgctggtgga cgcgctcggc ggcctgctgt cggccaccga
     1441 ggggcgcctg ggcgcccccg aggcgggcct gcgcgacgcc ctggccaacc tgcgcatggc
     1501 ctacgccgag gtggccgcgg ccgcggaggg ctccgccccc cgcactcccc ccggaaccga
     1561 gccggagggc gaggactcgg ggatcctgcg gccgccgagc gggctgtggg tgcccggcca
     1621 ggagtgacgg ccccggaagg cgaaaggccc cgcccggagg cggggccctg ccaaccttgc
     1681 gtgatgccgg acgtcaacga accgaaggcc cgtcctgctc ctgctcgtta cgcaagcggc
     1741 cgcacgttcg aggcctgtgg gcccttgggc ccgtcggtga tctcgaactc gacggcctgg
     1801 ccctcgttga gggtcttgta gccgtcgctc tggatggccg agaagtggac gaagacgtcg
     1861 tcaccgccgt tgcgggcgat gaagccgtag cccttctcgt tgctgaacca cttgacggtg
     1921 ccttcaggca acgtgcttcc ctcctgtcga ccccggcggc ctcgacaccg tttgctgagg
     1981 catgtcggcc gggccgcgcg gactgtaccg cgacgccaac ttgggcacaa gcgcaggcca
     2041 aaacgctcaa accgactggt caggcgacga tctcagacga cgatctcagg cgacgatctt
     2101 ggagatcggg agctccagga tgtcgaacgc cccggcggcc ttgagggtgg ggatgagccg
     2161 gttgaccccg gccttgtcga ccaccgtctc cacggcgtgg aagccggagt cggcgagctc
     2221 gttcacggtc ggggccttca tcgacggcag cacggccagc acccggtgaa ggtcggcgtc
     2281 ggccacgttc agcttgatca gcaccttgga ccgggccgtg gccgccccga cgagcagggt
     2341 gagcaggtcg tccatggcct ggcggcgctc ggggtcggcg gccgactcgg cgttggcgat
     2401 cacctccgtc caggaggtga gcagggtcgc gatcaccacc atgccgtggc tgcgcagggt
     2461 cgagccggtc tcggtgaggt cgacgaccgc gtccacgatc tcgggcacct tggcctcggt
     2521 ggccccgtag gaggggaaga tccgggccgg caggccccgc tcggcgaaga accgcctggt
     2581 gaggttgggg aactcggtgg agacgcgcag gcccggctgg agctcctcgg gccgggtgac
     2641 ctcgaggtcg cggtggacgg ccagcaccac cctgaccggg ttggaggtct gcttggagta
     2701 gggcagctcg gccaggctga ccaccttgct gcccgtctcc tcgatccagt cccggccggt
     2761 gatgccgagg tcgaagaagc cctcctcgac gtactggggg atctcctggg gccgcaggag
     2821 cgacacccgc tccaccctgg ggtcgtcgat ggcgccgtgg tagtcgcgct cgctcccccg
     2881 cttgacgggc aggtcggcgg cctcgaacag ggccagggtg gcccgctcca ggctcccctt
     2941 gggcagcacc agcttgagca cgacccgtct cctctcctga tgtcagtctt gcttgtcgat
     3001 cgcttccagc aggccggcca tctccagcgc acacacggcg gcctcggcgc ccttgttgcc
     3061 ggccttgccg ccggcccggt cgagcgcctg ctccatggtg tcgcaggtca gcaccccgaa
     3121 cgccaccggc acgccgctgg cccgggtgac ggcggccagg ccggcggcgg cctggccggc
     3181 gacgtggtcg aagtgggggg tggcgccgcg gaccacggcc ccgatggcca ccacggcggc
     3241 gtagcggccg gtcgcggcca gccgctcggc ggccaggggc agctcgaagg cgccc
//
LOCUS       DASSWR010000496         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_810000,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000496 DASSWR010000000
VERSION     DASSWR010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810000"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..547
                     /gene="mihF"
                     /locus_tag="VFO47_08305"
     CDS             230..547
                     /gene="mihF"
                     /locus_tag="VFO47_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET8788737.1"
                     /translation="MPLPTLTPEQRQQALEKAAEARRKRAELKAQLKSGNMSLATVLA
                     KDSDDTVGKMKVSSVLESMPGVGKVRARKIMEKLDISASRRVRGLGSKQKDALLAEFG
                     GKK"
     gene            612..1196
                     /gene="gmk"
                     /locus_tag="VFO47_08310"
     CDS             612..1196
                     /gene="gmk"
                     /locus_tag="VFO47_08310"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007098614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET8788738.1"
                     /translation="MLAGPSGVGKGAIVARLLAALPDLELSISATTRQPRAREDEGRH
                     YHFVERADFDDMVAAGGFLEWADIFGERYGTPREPVDRALAEGRDVLVEIDVQGARQV
                     KAAEPGAFMVFITPPSLQELERRLRTRGSETDQQIRGRLAKASDELAAEPEFDVTVVN
                     DDLETAAREVVEIVDRLRRRTINGGHTRVKERKH"
     gene            1196..1447
                     /gene="rpoZ"
                     /locus_tag="VFO47_08315"
     CDS             1196..1447
                     /gene="rpoZ"
                     /locus_tag="VFO47_08315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET8788739.1"
                     /translation="MIEPKIDELLARVDSKYTLVILAARRARQIIGYYSQLGEGIGEF
                     VPPMVDTGPNRKALSVALQELVEDKLNWERPAEVAESIK"
     gene            1525..>2348
                     /gene="coaBC"
                     /locus_tag="VFO47_08320"
     CDS             1525..>2348
                     /gene="coaBC"
                     /locus_tag="VFO47_08320"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET8788740.1"
                     /translation="MLLGVTGGIAAYKAAELARELQRAGAEVQPLLTADGERMVTRAT
                     FAALTGRRVPASVWDDPAAVEHVALARWGQVLVVAPATAHTLARLAAGLADDLLTNIA
                     LAFPGPVVVAPAMHTEMWEHPATRANLQTLEARGVRVVPPASGPLTSGDVGPGRLAEL
                     DDLVAGVAAALTPTQTLAGTRLLVSLGGTREPLDPVRYLGNRSSGRMGAAIVAEALRR
                     GAEVTAVAAATTVDPPAGARVIRVETAQQLYDAVLAEAGTQDVLVMAAAVADFRPKR"
BASE COUNT          357 a          854 c          850 g          287 t
ORIGIN      
        1 gtgggcgcgg ccgcccgggc ggtccgcgac caggccctcg cggccgcccc caaggcgggt
       61 ggggacgaac ccgcgaacca gccaaaagac ccggccgggc atggtcgatt tgacggtttg
      121 taaacagagg agggttctga ccagcgccga tcgattgtgg ccgccgccaa ggtatgcata
      181 gaatccgcga gtcttcgatc cagaacaacg aggtcacgag gaggcacgaa tgcccctgcc
      241 aaccctgacg cccgagcagc gccagcaggc gttggagaag gctgccgagg cgcgccgcaa
      301 gcgagctgag ctcaaggccc agctgaagag cggcaacatg agcctggcca ccgtccttgc
      361 caaggactcc gacgacaccg ttggcaagat gaaggtctcc agcgtgctcg agagcatgcc
      421 cggagtcggc aaggtgcgcg cccgcaagat catggagaag ctcgacatct ccgcctcccg
      481 ccgggtccgt ggcctgggct ccaagcagaa ggacgccctg ctcgccgagt tcggcggcaa
      541 gaagtagcaa gcaccccgcg ttggccaatg gtggggggtg ccagcagtcg ggtcggcgag
      601 gcaggctgat cgtgctcgcc ggcccgtcgg gggtcggtaa gggggccatc gtggccaggc
      661 tgctggccgc cctgcccgac ctggagctgt ccatctcggc caccacccgc cagccgcggg
      721 cccgcgagga cgagggccgt cactaccact tcgtggagcg ggccgacttc gacgacatgg
      781 tcgccgccgg cgggttcctc gagtgggccg acatcttcgg cgagcgctac ggcacgccgc
      841 gggagccggt cgaccgcgcc ctggccgagg gccgcgacgt cctggtcgag atcgacgtcc
      901 agggcgcccg gcaggtcaag gcggccgagc cgggggcctt catggtgttc atcaccccgc
      961 cgagcctcca ggagctggag cggcggctgc gcacccgggg cagcgagacc gaccagcaga
     1021 tccgcgggcg cctggccaag gcctccgacg agctcgccgc cgagcccgag ttcgacgtca
     1081 ccgtggtcaa cgacgatctg gaaacggccg cccgcgaggt ggtcgagatc gtcgaccggc
     1141 tgagacggcg taccatcaac ggcggtcaca cgcgtgtcaa ggagcggaag cactgatgat
     1201 cgaacccaag atcgacgaac tgctggcccg ggtcgactcc aagtacaccc tggtcatcct
     1261 ggccgccagg cgggccaggc agatcatcgg ctactacagc cagctcggtg agggcatcgg
     1321 cgagttcgtg ccgccgatgg tcgacaccgg ccccaaccgc aaggccctct cggtcgccct
     1381 ccaggagctg gtcgaggaca agctgaactg ggagcgcccg gccgaggtcg ccgagtccat
     1441 caagtaacgg tggcccagcc ggcagggggc accgggggct caaccggatc cccgcgcccg
     1501 ggatctgcgc tggttggggc ccgggtgctg ctcggggtga ccggggggat cgccgcctac
     1561 aaggcggccg agctggcccg cgagctgcag cgcgccgggg ccgaggtcca gccgctgctc
     1621 accgccgacg gcgagcggat ggtcaccagg gccaccttcg ccgccctcac cggccggcgg
     1681 gtgccggcct cggtctggga cgacccggcc gcggtcgagc acgtcgccct ggcccgctgg
     1741 ggccaggtcc tggtggtcgc cccggcgacc gcccacaccc tggcccggct ggccgccggc
     1801 ctggccgacg acctgctgac caacatcgcc ctggcgttcc ccggtccggt ggtggtcgcc
     1861 ccggccatgc acaccgagat gtgggagcac ccggccaccc gggccaacct ccagaccctg
     1921 gaggcgcgcg gggtccgggt cgtgcccccg gcgtcgggcc cgctgacctc cggcgacgtc
     1981 ggccccggcc gcctggccga gctcgacgac ctggtcgccg gcgtcgccgc cgccctcact
     2041 ccgacacaga ccctggccgg gacccggctg ctggtcagtc tgggcgggac ccgggagccg
     2101 ctcgacccgg tccgctacct cggcaaccgc tcctcgggcc ggatgggagc ggcgatcgtg
     2161 gccgaggcgc tccgccgcgg ggccgaggtc accgccgtgg ccgcggccac caccgtcgac
     2221 ccgccggccg gggcccgggt gatccgggtc gagaccgccc agcagctgta cgacgccgtg
     2281 ctcgccgaag ccggcaccca ggacgtgctg gtgatggcgg ccgcggtggc cgacttccgg
     2341 cccaagcg
//
LOCUS       DASSWR010000497         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_188346,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000497 DASSWR010000000
VERSION     DASSWR010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188346"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VFO47_08325"
     CDS             <1..1240
                     /locus_tag="VFO47_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8788741.1"
                     /translation="ALRKRSAAEGYYSLFMPEELVALRDAIRTFIQREVAPKEATYAQ
                     EITETGTFGALQQEKKALRKRSAAEGYYSLFMPEEAGGGGVGYLGLVLCVEECMRHGL
                     LLAMPGGVLPSVEGPTPILLDGTPSQQERYLQPTMRAEREACFALTEPDAGSDATRIR
                     TRAVRDNGGWVINGRKHFITHGAEADYVILFAVTDPGKGARGGISCFLVDTDTPGFEV
                     GRRQRTMYDEHQAELVFTDCRVGDDALLGKEGYGFYSAMRWINGGRVSIAAMAVGVAQ
                     HLYERMLEYAKVREAFGRRIGANQYVQGMIVDTLAELTQARLLVYDLAARLDSGADGR
                     QEAAMAKYVATEMVGRVADRAIQVFGGNGYMTEMGVERWARFVRAMRLYEGTSEILKT
                     NIARTLGLDEPSRVPAPAVQ"
     gene            1307..1549
                     /locus_tag="VFO47_08330"
     CDS             1307..1549
                     /locus_tag="VFO47_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788742.1"
                     /translation="MRTLRARSTLLLAAAAWTIWVWATRIWNIVGDPAHDAAFKAVHS
                     LLALVSIGFGIALAVVGLRMRREERAPEIETAGRAG"
     gene            1583..2200
                     /locus_tag="VFO47_08335"
     CDS             1583..2200
                     /locus_tag="VFO47_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788743.1"
                     /translation="MFIRILRGEAVDGAAALAEIDRWRHELGAGVLGWRRLTAGIGDG
                     GELVLALRYDTEAAARRDRDRPELAAWQSAAERHLAGPGRWYDCPVVHTMKAGDAGDA
                     GFLRVVQGRLADPIRLAAMRAEVERTLRDRAPHVLGVTVAEHADGTGFTELTYLTSER
                     ETRAADRQMPVEMAVQLGTVRSYVEALEEVELRRPLLASPAVPVG"
     gene            complement(2197..3390)
                     /gene="larC"
                     /locus_tag="VFO47_08340"
     CDS             complement(2197..3390)
                     /gene="larC"
                     /locus_tag="VFO47_08340"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET8788744.1"
                     /translation="MTFAYFDCFAGVAGDMVLGALLDAGGSAEALRAGLAGVPVDPFE
                     LEVTGVERGGIGATQVRVRAERSHVIRTWAYLRGALGEADLPAPVRDRSLAIFGRLAE
                     VEARIHRKPVEQVHFHEVGAVDAVVDIVGSAVLLHDLGVTEVWASPVATGTGLVRGDH
                     GMLPVPAPAVLELLAEVPIYSGGVTAELTTPTGAAILAASAVRFTDLPPMRVAATGYG
                     AGTRQHDELPNLLRVLLGERTEDGAAGDSGLVLEANLDDMTPELAPWVLERLFEAGAA
                     DVWYTPIHMKRGRPGITLSVLCTPGTDAAVRELLWRETSTLGVRGQPVRKWMLERRVL
                     TVSLPGGKVRVKLGLDGGRVVNVAPEYADCARLASATGRPLKDVMARAQAAALATLDA
                     EDRAP"
     gene            complement(3387..>3890)
                     /gene="larB"
                     /locus_tag="VFO47_08345"
     CDS             complement(3387..>3890)
                     /gene="larB"
                     /locus_tag="VFO47_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET8788745.1"
                     /translation="EHAEAVRAVAPDARWHPRSGMLVARAAEPDELRPAGTVAVVCAG
                     TGDLPVAEEAWVTAEAFGCRVDTLADVGVAGAHRLFAEVARLQAADVLVVVAGMEGAL
                     PSLVAGMVAAPVIAVPTSVGYGAAFDGLAALLAMLNSCAVGVSVVNIDNGFGAGALAA
                     RILRARK"
BASE COUNT          524 a         1508 c         1393 g          465 t
ORIGIN      
        1 ggccctgcgc aagcgctcgg ccgccgaggg ctactactcg ctgttcatgc ccgaggagct
       61 ggtcgcgctg cgggacgcga tccgcacctt catccagcgg gaggtcgccc ccaaggaggc
      121 gacctacgct caggagatca ccgagaccgg caccttcggc gccctccagc aggagaagaa
      181 ggccctgcgc aagcgctcgg ccgccgaggg ctactactcg ctgttcatgc ccgaggaggc
      241 cggcggcggc ggggtcgggt acctcggcct ggtcctctgc gttgaggagt gcatgcgcca
      301 cggcctgctg ctggccatgc ccggcggggt gctcccgagc gtcgagggac cgaccccgat
      361 cctgctcgac ggcacaccct cccagcagga gcggtacctc cagccgacca tgcgggccga
      421 gcgcgaggcc tgcttcgccc tgaccgagcc cgacgccggc tcggacgcga ccaggatccg
      481 cacccgggcc gtgcgcgaca acggcggctg ggtcatcaac ggccgcaagc acttcatcac
      541 ccacggggcc gaggccgact acgtgatcct gttcgccgtc accgacccgg gcaagggcgc
      601 ccgcggcggc atcagctgct tcctggtcga caccgacacc cccgggttcg aggtcggccg
      661 gcgccagcgg accatgtacg acgagcacca ggccgagctg gtcttcaccg actgccgggt
      721 cggtgacgac gccctgctcg gcaaggaggg ctacggcttc tactcggcca tgcgctggat
      781 caacggcggc cgggtctcga tcgccgccat ggccgtcggg gtcgcccagc acctctacga
      841 gcggatgctc gagtacgcca aggtgcggga ggcgttcggc cgccgcatcg gcgccaacca
      901 gtacgtccag ggcatgatcg tggacaccct ggccgagctg acccaggccc gcctgctggt
      961 ctacgacctg gcagcccgcc tcgactccgg cgccgacggc cgccaggagg cggccatggc
     1021 caagtacgtg gccaccgaga tggtcggcag ggtggccgac cgggccatcc aggtgttcgg
     1081 cggcaacggc tacatgaccg agatgggggt ggagcgctgg gcccggttcg tccgggccat
     1141 gcgcctgtac gaaggcacca gcgagatcct gaagaccaac atcgcacgga cgctcgggct
     1201 cgacgagccc tcgcgggtac cggctccggc ggtgcagtga ggcgcggcgc ctgaatcccc
     1261 gggggctccg ccccccggga ccccctggat tccgtaggat gagggcatgc gaaccctccg
     1321 tgcccgctcg accctgctcc tggccgcggc cgcctggacg atctgggtct gggcgacccg
     1381 tatctggaac atcgtcggcg accccgccca cgacgccgcc ttcaaggccg tgcacagcct
     1441 gctcgccctg gtctcgatcg gcttcgggat cgccctggcc gtggtcggcc tgcgcatgcg
     1501 ccgcgaggag cgcgcgcccg agattgagac ggcgggacgc gccgggtagg ctgagccgct
     1561 cgccgacctg gggggagccg ccgtgttcat ccggatcctg cgcggagagg ccgttgacgg
     1621 cgccgccgcg ctggccgaga tcgaccgctg gcgccacgag ctcggcgccg gggtgctcgg
     1681 ctggcggcga ctgaccgccg gcatcggcga cggcggcgag ctggtcctgg cgctgcgcta
     1741 cgacaccgag gcggcggccc gccgcgaccg cgaccggccc gagctggccg cctggcagtc
     1801 cgcggccgaa cgccacctgg ccggccccgg ccgctggtac gactgcccgg tggtccacac
     1861 catgaaggcc ggcgacgccg gcgatgcggg cttcctccgc gtggtccagg gccgcctggc
     1921 cgacccgatc cgcctggccg ccatgcgggc cgaggtcgag cgcaccctgc gcgaccgcgc
     1981 cccccacgtc ctcggcgtga ccgtggccga gcacgccgac ggcaccggct tcaccgagct
     2041 cacctacctc acctccgagc gcgagacccg ggccgccgac cgccagatgc cggtcgagat
     2101 ggccgtccag ctcggcaccg tgcgcagcta cgtcgaggcc cttgaagagg tcgagctgcg
     2161 caggccgctg ctggccagcc cggccgtgcc ggtcggctag ggggcgcggt cctcggcgtc
     2221 gagggtggcc agggcggccg cctgggccct ggccatcacg tccttgaggg ggcggccggt
     2281 ggccgaggcc aggcgggcgc agtcggcgta ctcgggggcg acgttgacca cccggccgcc
     2341 gtccaggccc agcttgaccc ggaccttgcc gccgggcagc gacaccgtga gcacccgccg
     2401 ctccagcatc cacttgcgga ccggctgccc gcgcaccccc agggtggagg tctcgcgcca
     2461 cagcagctcg cgcacggccg cgtccgtgcc cggggtgcac agcaccgaca gggtgatgcc
     2521 cgggcggccc ctcttcatgt ggatcggggt gtaccagacg tcggccgccc cggcctcgaa
     2581 cagccgctcc agcacccagg gggccagctc cggtgtcatg tcgtcgaggt tggcctccag
     2641 caccaggccg ctgtcgccgg ccgccccgtc ctcggtgcgc tcgcccagca gcacccggag
     2701 caggttgggc agctcgtcgt gctggcgggt cccggccccg tagccggtcg cggccacccg
     2761 catcggcggc aggtcggtga agcggacggc gctggcggcc aggatggccg ccccggtggg
     2821 ggtggtcagc tcggccgtga ccccgccgga atagatcggc acctcggcga gcagctcgag
     2881 gacggccggg gcgggcaccg gcagcatgcc gtggtcgccc cggaccagcc cggtcccggt
     2941 ggccaccggc gacgcccaca cctcggtgac gcccaggtcg tgcaggagca cggcgctgcc
     3001 aacgatgtcc acgaccgcgt cgacggcccc gacctcgtgg aagtggacct gctcgaccgg
     3061 cttgcggtgg atcctggcct cgacctcggc cagccggccg aagatggcca gcgagcggtc
     3121 gcgcaccggg gccggcaggt cggcctcgcc cagggccccg cgcagatagg cccaggtgcg
     3181 gatcacgtgc gagcgctcgg cccggacccg gacctgggtg gcgccgatgc cgccccgctc
     3241 gaccccggtc acctccagct cgaacgggtc gaccggcacc ccggccagcc cggcccgcag
     3301 cgcctcggcc gacccaccgg cgtccagcag cgcccccagc accatgtccc cggccacgcc
     3361 ggcgaagcag tcgaagtagg cgaaggtcat ttgcgggctc gcaggatccg cgcggccagg
     3421 gcgccggccc cgaacccgtt gtcgatgttg accacgctca ccccgaccgc gcacgagttc
     3481 agcatggcca gcagcgcggc cagcccgtcg aaggcggccc cgtacccgac gctggtcggg
     3541 acggcgatca ccggggccgc caccatcccg gcgaccagcg acggcagggc gccctccatc
     3601 ccggccacca ccacgagcac gtcggccgcc tggaggcgcg ccacctcggc gaacagccgg
     3661 tgggccccgg ccacgccgac gtcggccagg gtgtcgaccc ggcagccgaa cgcctcggcc
     3721 gtgacccagg cctcctcggc caccgggagg tcccccgtcc cggcgcagac caccgcgacc
     3781 gtcccggccg gccggagctc gtccggctcg gccgccctgg ccaccagcat ccccgaccgg
     3841 gggtgccagc gggcgtcagg ggccacggcc cggaccgcct cggcgtgctc
//
LOCUS       DASSWR010000498         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_837645,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000498 DASSWR010000000
VERSION     DASSWR010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837645"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VFO47_08350"
     CDS             complement(<1..1845)
                     /locus_tag="VFO47_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8788746.1"
                     /translation="MTDLPTGTVTFLFTDIEGSTGLLQTLGDRYPAVLDEHAAVVRRA
                     VAGGGGVEVSTHGDAFFAVFASPAGAVKAAVAAQRGLAAHDWSPGPPVRVRMGVHTGE
                     GVLGGDDYAGIDVHRAARIADAAHGGQVLLSDATRGLVQHSLDAGASLRDLGMHRLRG
                     IDEPERLHQLVVEGLPSDFPAPRTLDARPSNLPLQLTSFVGREAEIAEVEQLLGQTRL
                     LTLTGPGGSGKSRLALRVAGELLTRFRDGSCFVDLSPVVDPALVPAAVASALGVPEAA
                     GRPILDEVKEHLRNRELLQVIDNFEQVAEAGPVIEELLGAAPRLRTMVTSRVVLSLRG
                     EQEYPVPALGAVEAVNLFTERATAANPRFALTEGNAPVVAEITARLDGLPLAIELAAT
                     RTKVLTPTEILSRLRRRLALLTSGPRSLPERQRTLRAAIAWSYDLLDPVERRLFARLS
                     VFTGGWALASAEAVCDPEELGLDALDGLASLVDKSLIRRSEPPGQPSRFSMLETIREF
                     GLEQLEASGDLEPVQRRHAEHFLHLATEAEPHLIAADQGVWLDRCEQEHANLRAALRW
                     AIDRGQAGPAQAAAGALWRFWQQRGHLAEGRRWLDETLAMPSGQAPTAA"
     gene            1903..>2405
                     /locus_tag="VFO47_08355"
     CDS             1903..>2405
                     /locus_tag="VFO47_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8788747.1"
                     /translation="MLLEHKNAVVYGGAGSVGRAVARAFASEGARVFLAGRTLATLDE
                     VARELSGEGAEVETARVDALDERAVDEHADAVAERAGGIDVSFNAIGHGDVHGAPLLE
                     MSLDDFARPIATAMRTQFLTTRAAARHMTRRGSGVVMAITATTARLAIPEVGGTGVTF
                     DAIESQCR"
BASE COUNT          310 a          880 c          932 g          283 t
ORIGIN      
        1 ggccgcggtc ggcgcctgcc cggacggcat ggccagggtc tcgtcgagcc accgccggcc
       61 ctcggccagg tgcccccgct gctgccagaa ccgccacagg gcgccggcgg ccgcctgggc
      121 gggaccggcc tggccccggt cgatggccca gcggagggcg gcgcggaggt tggcgtgctc
      181 ctgctcgcag cggtcgagcc agacgccctg gtcggcggcg atgaggtggg gctcggcctc
      241 ggtggccagg tggaggaagt gctcggcgtg gcgccgctgg accggctcga ggtcgccgga
      301 ggcctcgagc tgctccagcc cgaactcccg gatggtctcc agcatcgaga accgcgacgg
      361 ctgcccgggc ggctcgctcc ggcggatcag cgacttgtcg accagcgagg ccagcccgtc
      421 gagggcgtca aggccgagct cctccgggtc gcacaccgcc tcggccgacg cgagcgccca
      481 gccgccggtg aacaccgaga gccgggcgaa caggcgccgc tcgaccgggt cgagcaggtc
      541 gtagctccag gcgatggccg cccggagggt ccgctggcgc tccggcaggc tccgcggccc
      601 ggaggtgagg agcgcaaggc gccgccggag gcgcgacagg atctcggtgg gcgtgagcac
      661 cttcgtccgg gtggcggcca gctcgatggc cagggggagg ccgtccagcc gggcggtgat
      721 ctcggccacc accggggcgt tcccctcggt cagcgcgaac cgggggttgg cggccgtggc
      781 ccgctcggtg aacaggttca ccgcctcgac cgcgccgagc gccgggaccg ggtattcctg
      841 ctcgccgcgc agcgacagca cgaccctgga ggtgaccatc gtccgcagcc tgggcgcggc
      901 gccgagcagc tcctcgatca cggggccggc ctcggccacc tgctcgaagt tgtcgatcac
      961 ctggaggagc tcgcggttgc ggaggtgctc cttgacctcg tcgaggatcg ggcgcccggc
     1021 cgcctcgggg acccccagcg cgctggccac cgccgccggg accagcgccg gatcgaccac
     1081 gggggagagg tcgacgaagc aggagccgtc ccggaaccgg gtgagcagct ccccggcgac
     1141 ccgcagggcc agccgggact tcccggatcc ccccggtccg gtcagggtga gcaggcgggt
     1201 ctggccgagg agctgttcca cctcggcgat ctccgcctcc cggcccacga acgaggtgag
     1261 ctgcagcggg aggttgctcg gcctggcgtc cagcgtccgg ggcgccggga agtcggacgg
     1321 gaggccctcg acgaccagct ggtggaggcg ctcgggctcg tcgatgcccc gcaggcggtg
     1381 catgcccagg tcgcgcaggg aggcgccggc atcgagggag tgctggacca gcccccgggt
     1441 ggcgtcggac aggagcacct ggccgccgtg ggcggcgtcg gcgatccggg ccgcccggtg
     1501 cacgtcgatg ccggcgtagt cgtccccgcc gaggacgccc tcgccggtgt ggacgcccat
     1561 ccggacgcgg accgggggac cgggcgacca gtcgtgggcg gccaggcccc gctgggcggc
     1621 gaccgcggcc ttcacggccc ccgccgggct ggcgaacacg gcgaagaagg cgtcgccgtg
     1681 ggtgctgacc tcgaccccgc cgccgccggc gacggcccgc cggacgaccg ccgcgtgctc
     1741 gtccaggacc gccgggtacc ggtcgcccag ggtctggagg aggccggtcg agccctcgat
     1801 gtcggtgaag aggaacgtca cggtcccggt cgggaggtcg gtcatccgct gactctatgc
     1861 tgcctatccc cggtccggca ctcgacacca aggaggacga aggtgctgct cgagcacaag
     1921 aacgccgtgg tctacggggg cgccgggtcg gtcggccgcg ccgtggcccg cgccttcgcc
     1981 agcgagggcg cgagggtctt cctcgccggc cgcaccctcg ccaccctcga cgaggtggcc
     2041 cgggagctct ccggcgaggg cgctgaggtc gagacggcgc gggtcgacgc cctcgacgaa
     2101 cgggccgtcg acgagcacgc cgacgccgtc gccgagcggg ccggcggcat cgacgtgtcc
     2161 ttcaacgcca tcggccacgg cgacgtccac ggcgcgccgc tgctcgagat gtcgttggac
     2221 gacttcgccc ggccgatcgc gaccgccatg cggacccagt tcctcaccac cagggcggcc
     2281 gcccgccaca tgaccaggcg ggggtcgggc gtggtcatgg cgatcaccgc gaccaccgcc
     2341 cggctggcca tccccgaggt gggcgggacc ggggtcacct tcgacgccat cgagagccag
     2401 tgccg
//
LOCUS       DASSWR010000499         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_470179,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000499 DASSWR010000000
VERSION     DASSWR010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470179"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFO47_08360"
     CDS             complement(<1..161)
                     /locus_tag="VFO47_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET8788748.1"
                     /translation="MSIEQREWTRLLAWEGCLNARDLGGYATADGRETRWGAVVRSDS
                     PAALTAAGRA"
     gene            complement(239..1015)
                     /locus_tag="VFO47_08365"
     CDS             complement(239..1015)
                     /locus_tag="VFO47_08365"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET8788749.1"
                     /translation="MTIEQREWTRQLAWEGCVNARELGGYPAADGRETRWAAVVRSDS
                     PAALTAAGRAALAGYGVRAIVDLRLPAELADHPNPFAEPGDHGIAYTNVSFIDPAFAP
                     PDAVTTLAEDYLQMLDRYRHAVAEAITAVARAPEGVVLVHCAAGKDRTGLVAGLLLAL
                     VGVPAETVAADYAMTGEALRPQLEAWLESDPDERAEREALLARYAPTAEVMLEVLDQL
                     TTHYGGVEPYLLEAGVSRDDLGRLRDRLLAPAPRGDRPGS"
     gene            1145..1483
                     /locus_tag="VFO47_08370"
     CDS             1145..1483
                     /locus_tag="VFO47_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET8788750.1"
                     /translation="MGEQRTAAVMEALASVYDPCCQEKGLSVVDMGLVRKVDLDGDQA
                     RVELLLTTGWCPFAANLVGQITERVAALPGMAGSTVEVVWDEPWTTDRLSDKARRTLR
                     FLPNPKEVGP"
     gene            1483..2484
                     /locus_tag="VFO47_08375"
     CDS             1483..2484
                     /locus_tag="VFO47_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8788751.1"
                     /translation="MGNDAIVFDGVAHVFNFDKKNALGPPGEMFINHLYAFHATLTPE
                     GQPVMASDDFLRQWTVDEIDEMVYRQSSTDMLCAMPLPLTDLFRDGLSPWEECAELAS
                     RRPDRTVFWGSVNPLEGRRALDLMERQVGEFGAKAFKFYNVRYDYGRPFPWRMDDPRV
                     AFPVFEKAQELGVNLIGVHKGVPLGPQPIEATQTWDMDGAAANFPDINFVIFHVGLPF
                     IDEVCWQLIRHPNLYASIAATINFIGRAPRQFAETIGKLLFWAGEDKIIYGSEAPIWQ
                     PQWALRKFWDFEIPQELCEGYGYPQLTEQAKRKILGENLLRLHGMDVEETRARLRAG"
     gene            2515..3339
                     /locus_tag="VFO47_08380"
     CDS             2515..3339
                     /locus_tag="VFO47_08380"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HET8788752.1"
                     /translation="MSGPARMFEVARNPDPASRLPYLVRLPVAGGDVVVKARESWPRT
                     GTVYCHPAGGGWPEEAELVERVPVRSCRRRGGAIDLVLDRPRERRSQLVFTRVRGRQV
                     IFWQSPRTARAARPGVRVPGRRAGGLQELTILVDTRERYPWRFGRQRAVSTERRALPA
                     GDYAVEAPGGGLAAVVERKTLADLAGSLADGSLSYLLAELAAMPRAAVVVEDRYAGVF
                     KLEHVQPGFVADLLGVLAVRYPAVPIVFCDTRPLAQEWAYRYLGAALAELTPGARG"
BASE COUNT          453 a         1257 c         1280 g          473 t
ORIGIN      
        1 gcccggccgg ccgcggtcag cgcggccggg ctgtcggagc gcaccaccgc cccccagcgg
       61 gtctcccgcc cgtccgcggt cgcgtagcca cccagatcac gggcgttcag gcatccctcc
      121 caggccagca gccgggtcca ctcgcgttgc tcgatcgaca cgtcccctcc gttcgcagcg
      181 tccgccgtcc agcctaccga cgcccggtga cgctccggcg gaacaggcgg ctgcccgcct
      241 acgacccagg ccgatcgccc cgcggggcgg gggcgagcag gcggtcgcgg aggcggccca
      301 ggtcgtcccg ggagacgccg gcctccagga ggtagggctc gaccccgccg tagtgggtcg
      361 tcagctggtc gaggacctcg agcatgacct cggcggtggg ggcgtagcgg gccagcaggg
      421 cctcgcgctc ggcccgctcg tccgggtcgc tctccagcca ggcctcgagc tgggggcgga
      481 gcgcctcgcc ggtcatggcg tagtcggcgg cgaccgtctc cgcgggcacc cccaccaggg
      541 cgagcagcag gcctgcgacc aagccggtcc ggtccttgcc ggccgcgcag tggaccagca
      601 cgacgccctc gggggcccgg gcgacggccg tgatcgcctc ggcgacggcg tggcggtagc
      661 ggtcgagcat ctgcaggtag tcctcggcga gggtggtgac ggcgtcgggg ggcgcgaacg
      721 ccgggtcgat gaaggacacg ttggtgtagg cgatcccgtg gtcgcccggc tcggcgaacg
      781 ggttggggtg gtcggccagc tcggccggca gccgcaggtc gacgatggcc cggacgccgt
      841 agccggccag ggcggcccgg ccggccgcgg tcagcgcggc cgggctgtcg gagcgcacca
      901 ccgccgccca gcgggtctcg cgcccgtccg ccgccggata cccgcccagc tcgcgggcgt
      961 tcacgcaccc ctcccaggcc agctgccggg tccactcacg ctgctcgatc gtcacggtcc
     1021 ctcccggtca cggagctcca gcggccagcc taccgtcgcg gtggtatgcg acctaccggt
     1081 tgacgggccg gatggagcgg accaaggttg gaggggccag gacgccgagg tccgcgaggt
     1141 ggagatgggc gagcaacgca ccgcggccgt gatggaggcg ctggcctccg tctacgaccc
     1201 ctgctgccag gagaagggcc tgtcggtggt cgacatgggc ctggtccgga aggtcgacct
     1261 ggacggcgac caggcacggg tcgagctgct gctgaccacc ggctggtgcc cgttcgcggc
     1321 caacctggtc ggccagatca ccgaacgggt ggccgccctg cccgggatgg ccggctcgac
     1381 ggtcgaggtc gtctgggacg agccgtggac caccgaccgg ctgtccgaca aggcccggcg
     1441 cacgctgcgc ttcctgccca accccaagga ggtcggcccg tgatcggcaa cgacgcgatc
     1501 gtgttcgacg gcgtggcgca cgtcttcaac ttcgacaaga agaacgccct cggccccccc
     1561 ggggagatgt tcatcaacca cctgtacgcc ttccacgcca ccctgacccc cgaggggcag
     1621 ccggtgatgg cgtccgacga cttcctgcgc cagtggaccg tcgacgagat cgacgagatg
     1681 gtctaccgcc agagctccac cgacatgctg tgcgccatgc cgctgcccct gaccgacctg
     1741 ttccgcgacg gcctctcgcc ctgggaggag tgcgccgagc tggccagccg ccggcccgac
     1801 cggacggttt tctggggctc ggtcaacccg ctggagggcc gccgcgccct ggacctgatg
     1861 gagcgccagg tcggcgagtt cggggccaag gccttcaagt tctacaacgt ccgctacgac
     1921 tacggccggc cgttcccctg gcgcatggac gacccccggg tcgccttccc ggtgttcgag
     1981 aaggcccagg agctgggcgt caacctgatc ggggtccaca agggcgtccc cctcggcccc
     2041 cagccgatcg aggccaccca gacctgggac atggacgggg cggcggccaa cttccccgac
     2101 atcaacttcg tcatcttcca cgtcgggctg ccgttcatcg acgaggtctg ctggcagctc
     2161 atccgccacc cgaacctgta cgcgtcgatc gccgccacga tcaacttcat cgggcgcgcg
     2221 ccgaggcagt tcgccgagac gatcggcaag ctgctgttct gggccggcga ggacaagatc
     2281 atctacggca gcgaggcgcc tatctggcag ccgcagtggg cgctgcgcaa gttctgggac
     2341 ttcgagatcc cgcaggagct gtgcgagggc tacggctacc cgcagctcac cgagcaggcc
     2401 aagcgcaaga tcctgggcga gaacctcctg cgcctccacg gcatggacgt cgaggagacg
     2461 agggcccggc tgcgcgccgg ctgacccgtc gggccggggt ggcagaatcg ccgcatgagc
     2521 ggccctgccc gcatgttcga ggtggcccgc aacccggacc cggcgtcgcg gctgccgtac
     2581 ctggtccgcc tgcccgtggc cggcggggac gtcgtggtca aggcccggga gtcgtggcca
     2641 cggaccggga ccgtgtactg ccacccggcc ggcggtgggt ggccggagga ggccgagctg
     2701 gtcgagcggg tcccggtccg ctcctgccgg cgccgaggcg gcgccatcga cctggtcctg
     2761 gaccggcccc gcgagcgccg ctcccagctc gtcttcaccc gggtccgggg ccggcaggtg
     2821 atcttctggc agtcgccccg gacggccagg gcggcccggc ccggggtgcg ggtgccgggg
     2881 cgccgggccg gcggcctgca ggagctgacc atcctggtcg acacgcgcga gcgctacccg
     2941 tggcggttcg ggcgccagcg ggcggtgtcg accgagcggc gggccctgcc ggccggcgac
     3001 tacgccgtcg aggcccccgg cggggggctg gccgcggtgg tggagcgcaa gacccttgcc
     3061 gacctggccg ggtcgctggc cgacggcagc ctgagctatc tgctcgccga gctggccgcg
     3121 atgcccaggg cggcggtcgt ggtcgaggac cgctacgccg gggtgttcaa gctggagcac
     3181 gtccagcccg ggttcgtggc cgacctgctg ggcgtgctgg ccgtccgcta cccggccgtc
     3241 ccgatcgtgt tctgcgacac ccgcccgctg gcccaggagt gggcctaccg ctacctcggg
     3301 gcggccctgg ccgagctcac tccaggggct cgggggtgag gtcgaggtcc tcctcgtcgc
     3361 cgaaggcgat gccgaggccg cgggcggtct ggatcatctc gtggtcgacc gggacggtgt
     3421 tgaccttccc ggcgcagtcg gccatggaga cggtcgagat ctc
//
LOCUS       DASSWR010000500         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_640797,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000500 DASSWR010000000
VERSION     DASSWR010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640797"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFO47_08385"
     CDS             <1..330
                     /locus_tag="VFO47_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788753.1"
                     /translation="LKDIDAHELARAVHLAAEGSAVIHPNLTRQFIEEIRQLTRGEQS
                     VSTLSAREVEVLQMIAYGSTNREVAEALHISPQTVKTYLERIFTKLGVSDRTRAVAVA
                     LKHGIVE"
     gene            338..961
                     /locus_tag="VFO47_08390"
     CDS             338..961
                     /locus_tag="VFO47_08390"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HET8788754.1"
                     /translation="MGEPLLFLLVAGAGVAVGLARGGSLRRLREPGVRAARLGVAFLA
                     VQSLVVVLPLRALGLPLVLGAVLLLASLVVLLGVARANGRLPGVPLLALGLLLNLLVV
                     LANLGMPVPAETLERAGIGVEQPAPDRPDPKHVLERGGTRLGLLGDRLAVRPLRTVTA
                     YGTVIELAGLFLLLQHLVRGPGPFRREDGTAHVVHEARTVPWTTWAE"
     gene            complement(989..1204)
                     /locus_tag="VFO47_08395"
     CDS             complement(989..1204)
                     /locus_tag="VFO47_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8788755.1"
                     /translation="MAQRRTSTRIEAPSYLRTAEVADILHVSPKTVSRWAKEGKLPFL
                     KTLGGHRRYPEAKIRELANDLREEPTG"
     gene            complement(1409..2365)
                     /locus_tag="VFO47_08400"
     CDS             complement(1409..2365)
                     /locus_tag="VFO47_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8788756.1"
                     /translation="MLVVVAGGAGFLGASLVDRLQAEGDELVVVDDLSHGHLANLAEA
                     RRRGGVRFHRMDVAQGGLATLADRVRADAWVHLATAIDPVRAWEDPTGEARAVVPGGI
                     EVLRAAAATGARAVLVSSGAYLYPAQLTGATRPGDEPRPVHPSGAARLALEAYAGAFA
                     ARGLDVVTLVLGTVYGPRQDPLGPGLVARAAWTMLQNQPPRVDGDGRQERDFLFVDDA
                     VDAIARAVHASPPPGRVLVGTGAATGVLDVVEQLAARIGWAGEPQWAPARPGDPRRSA
                     LDPAAAGKALGWQPWTRLDEGLGLTVDWLKARLPGHYRHIGR"
     gene            complement(2398..>2945)
                     /gene="lepB"
                     /locus_tag="VFO47_08405"
     CDS             complement(2398..>2945)
                     /gene="lepB"
                     /locus_tag="VFO47_08405"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET8788757.1"
                     /translation="AYVRQRRAFSDRGYPSGGLRKPALVTGVGALAAVTALGVWLRRA
                     RLEPVLVRGGSMRPTLEPGQRIAVAPLVRPPARGDLVVLSSGGREVVKRVVGLPGERV
                     RLRGGRLEVDGQAVPEPYLAEGSGAGELDLRLGPAEYLVLGDHRAASTDGRDFGPVAA
                     DALLARVAFAYWPPRRLRRRG"
BASE COUNT          358 a         1107 c         1101 g          379 t
ORIGIN      
        1 ctcaaggaca tcgacgccca cgagctggcc agggccgtgc acctggccgc cgagggctcg
       61 gcggtcatcc accccaacct gacccgccag ttcatcgagg agatccgcca gctcacccgg
      121 ggcgagcaga gcgtctccac cctctcggcc cgcgaggtcg aggtgctgca gatgatcgcc
      181 tacggctcca ccaaccgtga ggtggccgag gccctgcaca tctccccgca gacggtgaag
      241 acctacctgg agcggatctt caccaagctc ggcgtctccg accgcacccg ggccgtggcc
      301 gtagcgctca agcacggcat cgtagaatag tccgcccatg ggcgagccgc tgctgttcct
      361 gctggtggcc ggggccgggg tggccgtcgg cctggcccgg ggcgggtcgc tgcggcgcct
      421 gcgcgagccc ggggtcaggg cggcgcgtct cggcgtggcc ttcctggccg tccagtccct
      481 ggtggtggtg ctgccgctgc gggccctcgg cctcccgctc gtgctcggcg ccgtgctgct
      541 gctggcctcc ctggtcgtcc tgctgggggt cgcccgggcc aacgggcggc tgcccggggt
      601 gcccctgctc gccctcggcc tgctcctcaa cctgctcgtg gtcctggcca acctgggcat
      661 gccggtgccg gcggagacgc tggagcgggc cggtatcggg gtcgagcagc cggcccccga
      721 ccgccccgac cccaagcacg tcctggagcg cggcggcacc cggctggggc tgctgggcga
      781 ccggctggcc gtccggcccc tgcgcaccgt gaccgcgtac gggaccgtca tcgagctggc
      841 cggcctgttc ctgctcctgc agcacctggt ccgcgggccc ggcccgttcc ggcgcgaaga
      901 tggaaccgcc catgtcgtcc acgaggccag aacggtcccg tggacgacgt gggcggagtg
      961 acccccccgg gctggtctcg tccggccgct acccggtcgg ctcctcccgc aggtcgttgg
     1021 cgagctcgcg gatcttggcc tcgggatagc ggcggtgccc cccgagggtc ttgaggaagg
     1081 gcagcttgcc ctccttcgcc cacctcgaga ccgtcttggg cgagacgtgc aggatgtcgg
     1141 ccacctcggc ggtgcggagg tagctcggag cctcgatcct ggtgctggtt ctgcgttgtg
     1201 ccaccggttc gtccctttct cgagtccgaa ccccgacagg cgagccgagc agaaggacgt
     1261 gcggcggagt acgggtctgt cggtcaccct gctgggagca gtgcgaacag ggcgattatt
     1321 aggactggcc cgagcgccca ccatagcccg gttgggcggt tttagctcga aaattccacg
     1381 tagggttcga tcactaggga aggtcgagtc aacgccctat gtggcgataa tggcctggca
     1441 gccgggcctt gagccagtcg acggtgaggc cgaggccctc gtccaggcgg gtccacggct
     1501 gccagccgag ggccttgccg gcggccgccg ggtcgagggc cgagcgccgc gggtcgccgg
     1561 gacgggccgg ggcccactgg ggctcgccgg cccagccgat ccgggccgcc agctgctcga
     1621 ccacgtcaag gacgccggtc gccgccccgg tgccgaccag gacccggccg gggggcgggc
     1681 tggcgtggac ggcccgggcg atcgcgtcga cggcgtcgtc cacgaacagg aagtcgcgct
     1741 cctggcgccc gtcgccgtcc acccggggcg gctggttctg gagcatcgtc caggcggccc
     1801 tggcgaccag gccggggccg agcgggtcct gccgggggcc gtagaccgtc cccaggacga
     1861 gcgtgaccac gtcgaggccg cgggccgcga acgcgccggc gtaggcctcc agggccagcc
     1921 tggccgcgcc cgaggggtgc accgggcggg gctcgtcgcc gggacgggtg gctccggtga
     1981 gctgggccgg gtagaggtag gcgccggagg agacgagcac ggccctggcc ccggtggccg
     2041 cggccgcccg caggacctcg atcccgccgg ggaccacggc cctggcctcc cccgtcgggt
     2101 cctcccaggc ccggaccggg tcgatggccg tggccaggtg gacccaggcg tcggcccgga
     2161 cccggtcggc cagggtggcc aggccgccct gggccacgtc catgcggtgg aaccggaccc
     2221 cgccccggcg ccgggcctcg gccaggttgg ccaggtgccc gtgggagagg tcgtcgacca
     2281 ccacgagctc gtcgccctcg gcctggagcc ggtcgaccag gctcgccccc aggaacccgg
     2341 ccccaccggc caccaccacc agcacgtttc ctcccgcgtt gtgccgctcg gcgcagtcta
     2401 accccgacgg cgcagccgcc gtgggggcca gtaggcgaag gcgacccggg cgaggagggc
     2461 gtcggcggcg acggggccga agtcgcggcc gtcggtgctg gccgcccggt ggtcgcccag
     2521 gaccaggtac tcggccggac cgaggcgcag gtcgagctcg cccgctccgg agccttcagc
     2581 cagatacggc tcggggacgg cctggccgtc gacctccagc cggccgccgc gcagccgcac
     2641 ccgctccccg ggcagcccga ccacccgctt caccacctcc cggccgccgg agctgagcac
     2701 gaccaggtcg ccgcgggcgg gcgggcggac caggggggcg acggcgatcc gctggccggg
     2761 ctccagggtg ggtcgcatgc tgccgccccg gacgagcacc ggctccagcc gggcccggcg
     2821 cagccacacc ccgagcgcgg tcacggcggc cagcgcgccc acccccgtca ccagggccgg
     2881 cttccgcagg cccccggatg ggtagccacg gtcggagaag gctcgacgct gccgcacgta
     2941 ggcca
//
LOCUS       DASSWR010000501         4023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1177611, whole genome shotgun sequence.
ACCESSION   DASSWR010000501 DASSWR010000000
VERSION     DASSWR010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177611"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFO47_08410"
     CDS             <1..1110
                     /locus_tag="VFO47_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET8788758.1"
                     /translation="VVLGTVAGGLAAGGGAAAGLAVAIGIVQGLPLVVALSRPMAGFW
                     LSLAAGMLVSEAARAGGPGPVWAEPSLLAHLAVLALVGLEARPRVLAELWLLTLLAGA
                     ILVQRMPGLNASPDLAEMTTLSAVVLLAAGALAARRESRRRLAEQQLVNDAERARRAL
                     LEERTRIARELHDVVAHHMSVIAIQAEAAPYRVPDPPEELARSFAAIRATATEGLREL
                     RRVVGVLRADEAAEDHGPQPTLERLDELVANVEAAGLTATVEVTGTPGPLPAGVELSA
                     YRIVQEALSNAVRHAPGSSVRVALDHRPGGLVLRVVNGPARSQPVPPSRGSGHGVPGM
                     RERAAMLGGELTAEPRPDGGWAVTSVLPLPDGAGT"
     gene            1107..1769
                     /locus_tag="VFO47_08415"
     CDS             1107..1769
                     /locus_tag="VFO47_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788759.1"
                     /translation="MSVRVLIADDQAMVREGFSVLLGAQPGIEVVGEAVNGQDAVDKA
                     AELRPDVVLMDVRMPVLNGLEATRLLAAGGPKVLVVTTFDLDEYVYEALRAGASGFVL
                     KDASAQALAEAVRVVAAGDAMLSPSVTRRLLGAFARLGAPRPPERDQVEQLTERETEV
                     LTLVARGRSNAEIARDLVVAEQTVKTHMGRILTKLGLRDRTQAAVFAYETGLMRPGER
                     MT"
     gene            1769..3043
                     /locus_tag="VFO47_08420"
     CDS             1769..3043
                     /locus_tag="VFO47_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET8788760.1"
                     /translation="MELPWLEIGLVLLLVVVNAGFAGSEVALISLREGQLRRLEREDG
                     QGRLVARLARDPNQFLSTIQIGITLAGFLASAAAAVSLAQPLLPLLEFLGRAAEPVAV
                     LLITVVLTYLTLVVGELAPKRNALQRPERWARRAARPLAVVSTVTRPAVWLLSKSTDL
                     LVRLAGADPGRQREEVTEEEVRDMIVTGGVFRPEQRRLLSEAIEIGERRLSDILVPRR
                     DVVAIPAEATVQEAIQTLLETTHGRAPVCRGDLDEVLGLVTLQDLIGAEAAVADCVRP
                     VLALPESMGVLDALRRLQAERGQLAIVLNEYGGTEGIVTVEDLLEELVGEIYDEFDPD
                     SHGIHRQPDGSFILPGSFPVHDLPDLGVSLPEGDYATVAGLILERLGRIPVAGDTVQV
                     DRWRLEVLAMDRNAIGRVRLTPAGHPPPGSSP"
     gene            complement(3035..>4023)
                     /locus_tag="VFO47_08425"
     CDS             complement(3035..>4023)
                     /locus_tag="VFO47_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788761.1"
                     /translation="VGIDSIVDYQVLPLDPRVQQLQEAYVGKVVDTLHDLPNLLWEVA
                     NESSGGGKADPAFVEMLGLADTPEWGDSTAWQYWVIDVVKRHEAEMGYDAHPMGMTMQ
                     YPVPEQTRVNDPLLASRAEWISPGYDDEIFNDGGHPMAPGSPQSRWLEDPPAADGGKV
                     VISDSDHYAPGRGDALWAWRSFLRGHHPILMDFGIMGGVNPTDPAAGGPMSYAAFEPA
                     RYAMGDTLRFAERMNLIEMQPRGELSSTGYALANPGEEYLVLQPGATADPFTVRLAAG
                     TYTVEWFGVDSRQTMGADEVTVERDQDLGFTAPLAGSEPAVLYLRRPARQSG"
BASE COUNT          484 a         1466 c         1520 g          553 t
ORIGIN      
        1 gtggtgctcg ggacggtcgc cggcggcctg gccgccggcg gcggcgcggc ggccgggctg
       61 gccgtcgcca tcgggatcgt ccagggcctg cctctggtgg tcgccctgtc ccggccgatg
      121 gccgggttct ggctgtcgct ggccgccggg atgctggtca gcgaggcggc gcgggcggga
      181 gggcccgggc cggtgtgggc ggagcccagc ctgctggccc acctggccgt cctcgccctg
      241 gtcgggctgg aggcccggcc gcgggtcctg gccgagctgt ggctgctgac cctgctggcc
      301 ggggcgatcc tggtccagcg catgcccggg ctgaacgcct cccccgacct ggccgagatg
      361 accaccctgt cggccgtggt cctgctggcc gccggggcgc tggcggcacg ccgcgagagc
      421 cggcggcggc tggccgagca gcagcttgtc aacgacgccg aacgggcccg gcgggccctg
      481 ctcgaggagc gcacccggat cgcccgcgag ctgcacgacg tggtcgccca ccacatgtcg
      541 gtcatcgcca tccaggccga ggccgcgccc taccgggtgc cggacccccc cgaggagctg
      601 gcccggagct tcgccgccat ccgcgccacc gccaccgagg gcctgcggga gctgcggcgg
      661 gtggtcgggg tgctgcgggc cgacgaggcc gccgaggacc acgggccgca gcccaccctg
      721 gagcggctgg acgagctcgt cgccaacgtg gaagccgccg ggctgacggc cacggtggag
      781 gtcacgggca cgccggggcc gctgccggcc ggggtggagc tgtccgccta ccggatcgtg
      841 caggaggcgc tcagcaacgc cgtgcgccac gcacccggct cctccgtgcg ggtggcgctc
      901 gatcaccggc cgggcgggct ggtgctgcgg gtcgtcaacg gcccggcccg gtcccagccc
      961 gttcccccct cccgcgggtc cggacatggg gtgccgggca tgcgggaacg ggcggccatg
     1021 ctcgggggcg agctgaccgc cgagccccgg cccgacggcg gctgggcggt gacctcggtg
     1081 ctgccgctgc ccgacggtgc cggcacatga gcgtccgggt gctgatcgcc gacgaccagg
     1141 ccatggtccg cgaggggttc tcggtgctgc tcggcgccca gcccggcatc gaggtcgtcg
     1201 gcgaggcggt caacggccag gacgcggtcg acaaggccgc cgagctccgg cccgacgtcg
     1261 tcctcatgga cgtgcgcatg ccggtgctga acgggctgga ggcgacccgg ctgctcgccg
     1321 ccggcgggcc caaggtgctg gtggtgacca ccttcgatct ggacgagtac gtctatgagg
     1381 cgctgcgcgc cggggccagc gggttcgtgc tcaaggacgc ctccgcccag gccctggccg
     1441 aggcggtgcg ggtggtggcg gccggtgacg ccatgctgtc gccgtcggtg acgcgccggc
     1501 tgctgggggc cttcgcccgg ctcggcgcac cccggccgcc ggagcgggac caggtcgagc
     1561 agctcaccga gcgcgagacc gaggtgctga cgctggtcgc ccggggacgc tcgaacgccg
     1621 agatcgcccg ggacctggtg gtcgccgagc agacggtgaa gacccacatg ggccgcatcc
     1681 tgaccaagct gggcctgcgc gaccggaccc aggccgccgt cttcgcctac gagaccgggc
     1741 tgatgcgtcc gggcgagagg atgacctgat ggagctgcca tggcttgaga tcggactggt
     1801 gctgctgctg gtggtggtca acgccggctt cgcgggcagc gaggtcgccc tgatctcgct
     1861 gcgcgagggg cagctgcggc gcctggagcg ggaggacggc cagggccggc tggtggccag
     1921 gctggcccgc gaccccaacc agttcctgtc caccatccag atcggcatca ccctggccgg
     1981 gttcctggcc tcggcggccg cggccgtgtc cctggcccag ccgctcctgc cgctgctcga
     2041 gttcctcggc cgggcggccg agccggtggc cgtgctcctg atcaccgtcg tcctcaccta
     2101 cctgaccctg gtcgtcggcg agctcgcccc caagcgcaac gccctccagc gcccggagcg
     2161 gtgggcgcgg cgggcggccc ggcccctggc ggtggtctcc acggtgaccc gtccggccgt
     2221 ctggctcctg tcgaagtcga ccgacctgct ggtccgcctc gcgggggccg acccgggccg
     2281 gcagcgcgag gaggtgaccg aggaggaggt ccgcgacatg atcgtcaccg gcggcgtgtt
     2341 ccggcccgag cagcgccggc tgctgtccga ggcgatcgag atcggcgagc ggcgcctgag
     2401 cgacatcctg gtgccccgcc gggacgtcgt ggccatcccg gccgaggcca cggtccagga
     2461 ggcgatccag acgctgctcg agaccaccca cgggcgggcc ccggtctgcc gcggcgacct
     2521 cgacgaggtg ctcggcctgg tcaccctcca ggacctgatc ggcgccgagg ccgccgtggc
     2581 cgactgcgtc cggcccgtgc tcgccctgcc cgagtcgatg ggcgtgctcg acgccctgcg
     2641 gcgcctccag gccgagcgcg ggcagctggc catcgtgctc aacgagtacg gcggcaccga
     2701 ggggatcgtc accgtcgagg acctgctcga ggagctggtc ggggagatct acgacgagtt
     2761 cgaccccgac tcccatggca tccaccgcca acccgacggc tcgttcatcc tgcccgggtc
     2821 gttccccgtc cacgacctcc ccgacctggg cgtctccctg cccgagggcg actacgccac
     2881 cgtcgccgga ctgatcctgg agcggctggg acgcatcccg gtcgcgggcg acaccgtcca
     2941 ggtcgaccgc tggcggctgg aggtgctcgc catggaccgc aacgccatcg gccgggtgcg
     3001 tctgaccccc gctggccacc ccccaccggg cagctcaccc tgattgacgt gcggggcggc
     3061 gcaggtacag gaccgccggc tcggagccgg ccaacggagc cgtgaagccg aggtcctggt
     3121 cgcgctcgac cgtcacctcg tccgcaccca tggtctggcg gctgtcgacg ccgaaccact
     3181 cgaccgtgta ggtgccggcg gcaagccgca cggtgaacgg gtcggccgtc gcgcccggct
     3241 ggaggacgag gtactcctcg ccggggttgg ccagggcgta cccggtcgag ctgagctcgc
     3301 cgcgcggctg catctcgatc aggttcatcc gctcggcgaa gcgcagggtg tcgcccatgg
     3361 cgtagcgggc gggctcgaag gccgcatacg acatggggcc acccgcggcc gggtcggtcg
     3421 ggttgacgcc gcccatgatc ccgaagtcca tcaggatcgg atggtggccg cgcaggaacg
     3481 acctccaggc ccagagcgcg tcgccgcggc cgggggcgta gtggtcgctg tcagagatca
     3541 cgaccttgcc gccgtcggcg gcgggcgggt cctccagcca ccgggactgc ggggagccgg
     3601 gcgccatcgg gtgcccgccg tcgttgaaga tctcgtcgtc gtagccgggc gagatccact
     3661 cggcccggct ggccagcagc gggtcgttga ccctggtctg ttcggggacg gggtactgca
     3721 tggtcatccc catcggatgg gcgtcatagc ccatctcggc ctcgtgccgc ttcacgacgt
     3781 cgatcaccca gtactgccag gccgtggagt cgccccactc gggcgtgtcc gccaggccca
     3841 gcatctcgac gaaggccggg tcggccttgc cgccgcccga ggactcgttg gccacttccc
     3901 acagcaggtt gggcaggtcg tggagggtgt cgaccacctt gccgacgtag gcctcctgga
     3961 gctgctggac ccgcgggtcc aggggcagga cctggtagtc gacgatcgag tcgatcccga
     4021 ccc
//
LOCUS       DASSWR010000502         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_612066,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000502 DASSWR010000000
VERSION     DASSWR010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612066"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1701)
                     /locus_tag="VFO47_08430"
     CDS             complement(154..1701)
                     /locus_tag="VFO47_08430"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HET8788762.1"
                     /translation="MPSSPPSDLGLAAKSIAYHPTEAELRSYTDAMPQARQTEYGNVN
                     VQTKVVSRSKGSTYVVTDRPGEHSDQTIDREEGARLAALQDEYIRGQDMVVVDGFVGH
                     AGPYRTPARLIIEKANANIAGMQRFLYYDPVEGDTGHEPEITMIYTPNLAAPGYPNDR
                     VIAVDLEAGVTRVLNSDYFGESKKGGLRMWNRKVYEGGGLAMHAGCKVIPTSDGEKTM
                     LIVGLSGTGKTTTTFTKQNGSQAVQDDFIGLFAGGRIVSTEDGCFAKTFALSAEDEPA
                     IHGAVTKPDAYLENVSQHGDKIDFFDTSYTQNGRAVFNMRSIDNYPADRVPPASFLLI
                     LNRNENIIPGVARLNIEQAAGYFMLGETQGTSAGGRDEEGKSLRVPGTNPFFPLRHEQ
                     QGNRLAELLETHPLEVFLLNTGRVGGPEDDDRSKKVRIPHSSAIVRAIAEGSITWDED
                     PDFGYQVASEVPGIDDPELLQPRRLYERQGRSDEYRRQVERLKAERADYLAEFPGLDE
                     AIVKAVS"
     gene            complement(1739..2398)
                     /locus_tag="VFO47_08435"
     CDS             complement(1739..2398)
                     /locus_tag="VFO47_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET8788763.1"
                     /translation="MAARQRRFLDRLEAGGLLAEVVAGLVSPPVLVLGIPRGGVVVAA
                     RVAARLGAPLDVVVPRKVGAPGNPELAVGAVADGVQAIDEPAVRRLGLDMAEVRAEAA
                     RQTAEVARRTAAYRQGLPPLVLAGRTAVLVDDGVATGWTCAAAASYTRRAGAARVVVA
                     VPVGPVGLAERLRPVVDEVVVLVTPDPYLNVGQAYERFPQVDDDEVLRCLQEGRAHSP
                     R"
     gene            2592..2825
                     /locus_tag="VFO47_08440"
     CDS             2592..2825
                     /locus_tag="VFO47_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET8788764.1"
                     /translation="MVTAYILIQTEVGKAAQVAKEIAEIKGVQQAEDVTGPYDVIVRA
                     EARNVDDLGKLVVARVQAVDGITRTLTCPVVHL"
     gene            complement(2822..3946)
                     /gene="serC"
                     /locus_tag="VFO47_08445"
     CDS             complement(2822..3946)
                     /gene="serC"
                     /locus_tag="VFO47_08445"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HET8788765.1"
                     /translation="MTDQKSTPILPPELRPRDGRFGSGPSKVRQEAVAALAAAAPNFL
                     GTSHRQAPVRSVVGRIRAGLAELFDLPAGYEVALGNGGSTLFWDLATFSLIERRSQHL
                     VFGEFSAKFAAAATAAPHLDEPQLIVSPFGSHPSPRPEPLVDLYALTHNETSTGVAMP
                     IRRPPGAGGLVCVDATSAAGGLAVDPREFDVYYFAPQKSFAADGGLWLACLSPAAVER
                     AGRVAASGRHIPAMLDLGVALEQSRLDQTLNTPALATLFLLANQLDWLLEQGGLAWAT
                     KRCDESAGIVYGWAEASGYATPFVTDPAMRSHTVATVDLDPSVEAAAVSRALRANEIL
                     DTESYRKLGRNQLRIAMFPAIEPGDLDRLTRAVDWLVERL"
     gene            3998..>4317
                     /locus_tag="VFO47_08450"
     CDS             3998..>4317
                     /locus_tag="VFO47_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8788766.1"
                     /translation="MPRISRRVAAIAESATLAIDAKAKALKADGEDVIGFGAGEPDFP
                     TPPQIVEAAVAACQEPRYHHYTPGAGLLELRAAIAAKTARDSGLRVAPSHVMVTNGGK
                     HALF"
BASE COUNT          585 a         1490 c         1623 g          619 t
ORIGIN      
        1 agcgcctcca gagcgtgctc gctctcggcc tggttgagcc ggcagccgag ggtcttgaac
       61 gccaccttgg gcgccgcggg cgcggttggc tcagccattg gtctgctccg ccggggggct
      121 accgcttcgc tccatgagcc ccccggaccc ccctcaggac accgccttga cgatggcctc
      181 gtccaggccc gggaactcag ctaggtagtc ggcccgctcg gccttgaggc gctcgacctg
      241 gcggcggtac tcgtcggagc ggccctggcg ctcgtacagc cggcggggct ggagcagctc
      301 ggggtcgtcg atgccgggca cctcggaggc gacctggtag ccgaagtcgg ggtcctcgtc
      361 ccaggtgatg gagccctcgg cgatggcccg cacgatggcc gaggagtgcg ggatccgcac
      421 cttcttggag cggtcgtcgt cctcggggcc gccgacccgc ccggtgttga gcaggaacac
      481 ctccagcggg tgggtctcca gcagctcggc cagccggttg ccctgctgct cgtggcggag
      541 cgggaagaac gggttggtcc ccggcacccg cagcgacttg ccctcctcgt ccctgccgcc
      601 ggccgaggtg ccctgggtct cgccgagcat gaagtagccg gccgcctgct cgatgttgag
      661 ccgggccacc cccgggatga tgttctcgtt gcggttgagg atcagcagga acgacgccgg
      721 cgggacccgg tcggccgggt agttgtcgat cgagcgcatg ttgaacacgg cccggccgtt
      781 ctgggtgtag gaggtgtcga agaagtcgat cttgtcgccg tgctgggaga cattctccag
      841 ataggcgtcg ggcttggtca ccgccccgtg gatggccggc tcgtcctcgg ccgagagggc
      901 gaaggtcttg gcgaagcagc cgtcctcggt ggagacgatc ctgcccccgg cgaacagccc
      961 gatgaagtcg tcctggaccg cctgggagcc gttctgcttg gtgaaggtgg tggtggtctt
     1021 gcccgtgccc gagaggccga cgatcagcat ggtcttctcg ccgtcggagg tcgggatgac
     1081 cttgcagccg gcgtgcatgg ccagcccgcc gccctcgtag accttgcggt tccacatccg
     1141 caggccgccc ttcttcgact ccccgaagta gtcggagttg agcacccggg tgacgccggc
     1201 ctccaggtcg acggcgatca cccggtcgtt cgggtagccg ggggcggcca ggttgggggt
     1261 gtagatcatg gtgatctcgg gctcgtggcc ggtgtcgccc tcgaccgggt cgtagtagag
     1321 gaaccgctgc atgccggcga tgttggcgtt ggccttctcg atgatcagcc gggccggggt
     1381 gcggtagggg ccggcgtggc cgacgaagcc gtccaccacc accatgtcct ggccgcggat
     1441 gtactcgtcc tggagggcgg ccagccgggc cccctcctcc cggtcgatgg tctggtcgct
     1501 gtgctcgccg ggccggtcgg tgaccacgta ggtcgacccc ttggagcggg acaccacctt
     1561 ggtctggacg ttgacgttgc cgtactcggt ctgtcgggcc tggggcatgg cgtcggtgta
     1621 ggaccgaagc tcggcctcgg tcgggtggta ggcgatcgac ttggcggcaa ggcccaggtc
     1681 ggacggtggc gagctcggca tctaggccct ccaacgttgg agacgagagt tccccagtct
     1741 agcgtggcga atgggctcgc ccttcctgga gacagcgcag gacctcgtcg tcgtccacct
     1801 gggggaaccg ctcgtaggcc tggccgacgt tgaggtacgg gtccggggtg accagcacga
     1861 ccacctcgtc gaccaccggc cgcaaccgct cggccaggcc gaccgggccg acgggcacgg
     1921 cgaccacgac ccgggcggcg ccggcccggc gggtgtagga ggcggcggcc gcgcaggtcc
     1981 agccggtcgc caccccgtcg tcgaccagca ccgcggtccg gccggccagg acgagcgggg
     2041 gcaggccctg gcggtaggcg gcggtccgcc tggccacctc ggccgtctgg cgggcggcct
     2101 cggcccggac ctcggccatg tcgaggccga ggcggcgcac ggccggctcg tcgatcgcct
     2161 gcaccccgtc ggcgacggcg ccgacggcca gctcggggtt gccgggggcg ccgaccttgc
     2221 ggggcacgac cacgtcaagg ggagcgccga gccgggccgc gacccgggcg gccacgacca
     2281 cgccgccccg ggggatgccg aggaccagca cgggggggct gacgagaccg gccacgacct
     2341 cggccagcag cccgccggcc tccagccggt cgaggaatcg ccgctgccgt gccgccatct
     2401 cgctcccggt cggggaaggc aagcataccg agccttgcgc tttcgtgaaa cgcctggccg
     2461 gtcgcttcct gaccggcccc tgtcccgccc ttggcacacc caggaccccc gtgtaccatg
     2521 cgcagacctt tcgaccccgg tgcagccgtg ggcgcgccgt tgcggcggca tgatcaagga
     2581 ggtcgttgct tgtggtgacg gcatacatcc tcatccagac cgaggtcggc aaggccgccc
     2641 aggtcgccaa ggagatcgcc gagatcaagg gcgtccagca ggccgaggac gtcaccggcc
     2701 cctacgacgt gatcgtccgc gccgaggccc gcaacgtcga cgacctgggc aagctcgtgg
     2761 tggcgagggt ccaggcggtg gacggcatca cccgcaccct cacctgcccg gtggtccacc
     2821 tctagaggcg ttccaccagc cagtcgaccg cccgggtcag ccggtccagg tctcccggct
     2881 cgatcgccgg gaacatggcg atgcgcagct ggttgcgccc cagcttgcga tacgactcgg
     2941 tgtcgaggat ctcgttggcc cgcagcgccc gcgacaccgc cgccgcctcg accgacgggt
     3001 cgaggtcgac ggtggccacg gtgtggctgc gcatggccgg gtcggtcacg aacggggtgg
     3061 cgtagccgct ggcctcggcc cagccgtaga cgatcccggc cgactcgtcg cagcgcttgg
     3121 tcgcccaggc caggccgccc tgctccagca gccagtcgag ctggttggcg agcaggaaca
     3181 gggtggccag ggcaggggtg ttgagggtct ggtcgagccg tgactgctcc agggccaccc
     3241 cgaggtcgag catggccggg atgtggcggc cggaggcggc gacccgcccg gcccgctcga
     3301 cggccgccgg cgacaggcag gccagccaga ggccgccgtc ggcggcgaac gacttctgcg
     3361 gggcgaagta gtagacgtcg aactcgcgcg ggtcgacggc cagcccgccc gcggccgagg
     3421 tggcgtcgac gcagaccagg ccgccggcgc cgggcggccg ccggatcggc atggccaccc
     3481 cggtggaggt ctcgttgtgg gtcagggcgt acaggtcgac cagaggctcg ggacgcgggg
     3541 aggggtggct gccgaacggg ctgacgatca gctggggctc gtccaggtgg ggggcggcgg
     3601 tggcggcggc ggcgaacttg gccgagaact cgccgaacac caggtgctgg ctgcgccgct
     3661 cgatcaggga gaaggtggcc aggtcccaga agagggtcga cccgccgttg ccgagggcga
     3721 cctcgtagcc ggcggggagg tcgaacagct cggccagccc ggcccggatg cggcccacga
     3781 ccgagcggac cggcgcctgg cggtggctgg tcccgaggaa gtttggggcg gccgcggcca
     3841 gggcggccac cgcctcctgg cggaccttgg aggggccgct gccgaagcgg ccgtcccgcg
     3901 ggcgcagctc gggagggagg atcggggtgg atttctggtc ggtcatgcgg ccactctatc
     3961 ctcgctcgac aaccgtgccg cctggacgga gcagccagtg ccaaggatct cccgccgcgt
     4021 cgccgccatc gccgagtcgg ccaccctggc catcgacgcc aaggccaagg cgctcaaggc
     4081 ggacggcgag gacgtgatcg gcttcggcgc cggcgagccc gacttcccga caccgcccca
     4141 gatcgtcgag gcggcggtgg cggcctgcca ggagccccgc tatcaccact acaccccggg
     4201 ggccggcctg ctcgagctgc gggcggcgat cgcggccaag acggcccgcg actcgggcct
     4261 gcgggtcgcg ccgagccacg tgatggtcac caacggcggc aagcacgcgc tgttcca
//
LOCUS       DASSWR010000503         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1090701, whole genome shotgun sequence.
ACCESSION   DASSWR010000503 DASSWR010000000
VERSION     DASSWR010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090701"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /gene="nuoG"
                     /locus_tag="VFO47_08455"
     CDS             complement(<1..1556)
                     /gene="nuoG"
                     /locus_tag="VFO47_08455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HET8788767.1"
                     /translation="MADKPTTVKVTIDGQELEVPPGTLVIRAAEQLGTIVPRFCDHRL
                     LAPLGACRQCLVEVVGQRKPLTACTTTVTDGMEVKTQVTSEVARAGQEGNLELLLINH
                     PLDCPMCDKGGECPLQDQTLAHGPGETRFVETKRRYEKPIKISETVLLDRERCVLCAR
                     CTRFSSEIAGDPFIELFERGALEQVAIYEDEPYESYYSGNVVQICPVGALTSPSYRFQ
                     ARPFDLASTDGVCNHCASGCNLRIDARRGLVTRQLARDNDDVNEAWNCDRGRYGFAYA
                     QSPERVLIPHLSEDGKLRTASWSEALRRAADGIRAARDKGVGVLVGGHLTDQDAYALQ
                     KLARVALGTNNVDARAWPEDEAGAAAAASVAGRGPDEGMPAYRDLEAARAVVVVDLDP
                     HEESPILWLRLRKAAMRRGVPVVAVGSRPGRLAEFATVVATRRGRSVRRGLPLRYLSP
                     RRSMPPSAAGRRRRRPGPAAPGPRPGRRAGWRRRWRTRPAAPAGNRPRPPARPGAWPP
                     CAAGARGWATP"
     gene            complement(1564..2859)
                     /gene="nuoF"
                     /locus_tag="VFO47_08460"
     CDS             complement(1564..2859)
                     /gene="nuoF"
                     /locus_tag="VFO47_08460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET8788768.1"
                     /translation="MTDQVKVLTSRWGEREAFRLDRFTELDGYASLDAAFERSPDDLV
                     QLVKDSGLRGRGGAGFPTGMKWGFLAKGTGKPTYLVVNADESEPGTFKDMELLERDPH
                     ALVEGVIVSSYAIGCRNAFIYLRGECGWPGRRLQAAIDAAYDKGLLGRDVKGSGFDLD
                     VVLHRGAGAYICGEETALLSSLEGQRGQPKLRPPFPAVEGLYRAPTTVNNVETIATVP
                     WIVRKGVDWFRSMGSEKSPGPKLFSVSGDVVNPGNFEFPLGVPAREVIMGAAGGMRDG
                     KTLKAWTPGGSSTPMLTADHLDTAMDFEGIQGAGSLLGTAAVMVFSEDVCMVRACLNF
                     TEFYAHESCGKCTPCREGTDWMRKILGRLETGQGRPDDMDTLKDICDSIFGRSFCALG
                     DGATSPIVSALKYFRDEFDEHVSGGGCPFPGARVTVGAH"
     gene            complement(2856..3698)
                     /locus_tag="VFO47_08465"
     CDS             complement(2856..3698)
                     /locus_tag="VFO47_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET8788769.1"
                     /translation="MPLSDQTLTRAEELIARYPQPRSALLPILFLLQAEDGYVSPAGV
                     AQAAQLLGLTKAEVGAVATFYTMFRRRPVGKYLVSVCKTLPCQLRGSREISAAMAERL
                     EVPLGGTDGSGMVTVEEVECLAACDGAPVVQVNTENYERLSTSEAMELLEALLRDELP
                     PPTWAEDGSVTEPTTAGLHWRLAGLGDPPAGLGAPAAEPEERSIGREPSPPEPTEQRE
                     AGEPQPQEGAGAAARDPHPPVDGAPAGRVADGGTESDRPSTAPSDEGPAPEDKPRPQA
                     GEGR"
     gene            complement(3724..>4549)
                     /locus_tag="VFO47_08470"
     CDS             complement(3724..>4549)
                     /locus_tag="VFO47_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HET8788770.1"
                     /translation="EREEILDILEDVTGLRMNHAWVRPAGVSQDLPDGTEDKIRAFVR
                     DMRSKADEYETLLTNNPIWLERTKGIGHLALDELIGLGTTGPMLRAAGLDHDLRKAEP
                     YCGYEQYEFDVPTATGSDVYARYQVRLAEMRESLRIVEQVLDSLPGGPVMSTDPKVGW
                     PAKLALGPDGLGNSPAHVQHIMGESMEALIHHFKQVTEGIKVPRGEVYVPVESPRGEL
                     GYHVVSDGGNLPYRVHVRDPSFVNLQTGTPMAIGLLVADIIAVMSSIDPVLGGVDR"
BASE COUNT          577 a         1620 c         1692 g          660 t
ORIGIN      
        1 ggagtcgccc atcctctggc tccggctgcg caaggcggcc atgcgccggg gcgtgccggt
       61 ggtcgcggtc ggttcccggc cggggcggct ggccgagttc gccaccgtcg tcgccaccca
      121 gcccggcggc caggccgagg ccctggagcg gctgggccgg ggcgacggcg acgccggccg
      181 gctgctgaag gaggcattga acgccggggg ctcaggtagc ggagcggtag cccccggcgg
      241 actgatcgtc ctcgccgggt ggcgaccacg gtggcgaact cggccagccg ccccggccgc
      301 gacccgacgg cgaccacggg cacgccgcgg cgcatggccg ccttgcgcag ccgcagccac
      361 aggatgggcg actcctcgtg ggggtcgagg tcgacgacca ccaccgcccg ggccgcctcc
      421 aggtcccggt aggccggcat cccctcgtcg gggccccgcc cggcgaccga cgccgccgcg
      481 gccgccccgg cctcgtcctc cggccaggcg cgggcgtcga cgttgttggt gccgagcgcg
      541 acccgggcca gcttctgcag ggcgtaggcg tcctggtcgg tgaggtggcc gccgaccagg
      601 accccgactc ccttgtcgcg ggccgcgcgg atgccgtcgg cggcccggcg cagcgcctcc
      661 gaccaggagg cggtccgcag cttgccgtcc tcggacaggt gcgggatcag cacccgctcc
      721 ggggactggg cgtaggcgaa gccgtagcgg ccccggtcgc agttccaggc ctcgttgacg
      781 tcgtcgttgt cgcgggccag ctggcgggtg accaggcccc ggcgggcgtc gatgcgcagg
      841 ttgcagccgg acgcgcagtg gttgcagacg ccgtcggtcg aggccaggtc gaacggccgg
      901 gcctggaacc ggtaggacgg tgaggtcagc gcccccaccg ggcagatctg gaccacgttg
      961 ccgctgtagt agctctcgta cggctcgtcc tcgtagatgg ccacctgctc cagcgccccc
     1021 cgctcgaaca gctcgatgaa cgggtcgccg gcgatctcgc tggagaagcg ggtgcagcgg
     1081 gcgcagagca cgcagcgctc ccggtccagc aggaccgtct cggagatctt gatcggcttc
     1141 tcgtagcggc gcttggtctc cacgaaccgg gtctcccccg gcccgtgggc cagggtctgg
     1201 tcctggagcg ggcactcgcc gcccttgtcg cacatggggc agtcgagcgg gtggttgatc
     1261 agcagcagct cgaggttgcc ctcctggccg gcccgggcca cctcggaggt gacctgggtc
     1321 ttgacctcca tgccgtcggt gaccgtggtc gtgcaggcgg tcagcggctt gcgctggccg
     1381 acgacctcga ccaggcactg gcggcacgcc cccagggggg ccagcagccg gtggtcgcag
     1441 aaccggggca cgatcgtgcc cagctgctcg gcggcccgga tcaccagcgt gcccggaggg
     1501 acctccagct cctggccgtc gatggtgacc ttcacggtgg tcggcttgtc ggccatgcgg
     1561 ctcctaatgg gcgccaacgg tgacgcgcgc ccccgggaag gggcagccgc caccgctgac
     1621 gtgctcgtcg aactcgtcgc ggaagtactt cagggcggag acgatggggc tggtcgcccc
     1681 gtcgccgagg gcgcagaagg agcgcccgaa gatcgagtcg cagatgtcct tgagggtgtc
     1741 catgtcgtcg ggtcggccct ggcccgtctc gagccggccc aggatcttgc gcatccagtc
     1801 ggtgccctcg cggcaggggg tgcacttgcc gcaggactcg tgggcgtaga actcggtgaa
     1861 gttcaggcac gccctgacca tgcagacgtc ctcggagaag accatcaccg cggccgtgcc
     1921 gagcaggctg ccggcgccct ggatgccctc gaagtccatg gcggtgtcga ggtggtcggc
     1981 ggtcagcatc ggggtcgagc tgccccccgg ggtccaggcc ttgagggtct tgccgtcgcg
     2041 catcccgccg gccgcgccca tgatcacctc gcgggcgggg acgccgaggg ggaactcgaa
     2101 gttgcccggg ttgacgacgt cgcccgacac gctgaacagc ttcggcccgg gcgacttctc
     2161 gctgcccatg gaccggaacc agtcgacccc cttgcggacg atccagggca cggtggcgat
     2221 cgtctccacg ttgttgacgg tcgtcggggc ccggtacagg ccctcgacgg ccgggaaggg
     2281 cgggcgcagc ttgggctggc cgcgctggcc ctccagcgag gacagcaggg ccgtctcctc
     2341 gccgcagatg taggccccgg cgccccggtg caggaccacg tccaggtcga acccggagcc
     2401 cttgacgtcc cggccgagca gccccttgtc gtaggcggcg tcgatcgccg cctggagccg
     2461 gcggcccggc cagccgcact cgccccgcag gtagatgaag gcgttgcggc agccgatggc
     2521 gtacgaggac acgatcacgc cctcgaccag ggcgtgcggg tcccgctcga gcagctccat
     2581 gtccttgaag gtgccgggct cgctctcgtc ggcgttgacc accaggtagg tcggcttgcc
     2641 ggtccccttg gccaggaagc cccacttcat gcccgtcggg aagccggccc cgccccggcc
     2701 ccgcagcccc gagtccttga ccagctggac caggtcgtcg ggggaccgct cgaaggcggc
     2761 gtcgaggctg gcgtagccgt ccagctcggt gaaccggtcg agcctgaagg cctcccgttc
     2821 cccccagcgg gaggtcagca ccttgacctg gtcggtcatc ggccctcacc cgcctgcggc
     2881 cgcggcttgt cctcgggggc cggcccctcg tccgaagggg ctgtggacgg ccgatcgctc
     2941 tccgtcccgc cgtcggctac cctcccggca ggggccccat ccaccggcgg gtgagggtcc
     3001 cgcgcggccg cacctgctcc ttcctggggc tggggctcgc cggcctcgcg ctgctcggtc
     3061 ggctcgggcg gcgatggctc acgaccgatg ctgcgctcct ctggctcggc cgcaggggct
     3121 ccgaggccgg ccggggggtc gccgaggccg gccaggcgcc agtggagccc ggccgtggtg
     3181 ggctcggtga ccgacccgtc ctcggcccag gtgggcggcg ggagctcgtc gcggagcagg
     3241 gcctcgagca gctccatggc ctcggaggtg gacagccgct cgtagttctc cgtgttgacc
     3301 tggaccaccg gggcgccgtc gcaggcggcc aggcactcca cctcctcgac ggtgaccatc
     3361 ccgctcccgt cggtgccgcc caggggcacc tccagccgct cggccatggc cgccgagatc
     3421 tcccgcgacc cgcggagctg gcagggcagc gtcttgcaca ccgacaccag gtacttcccc
     3481 accgggcggc gccggaacat ggtgtagaag gtggccaccg ccccgacctc ggccttggtc
     3541 aagcccagga gctgggcggc ctgggcgacg ccggccggcg acacgtagcc gtcctccgcc
     3601 tgcagcagga acaggatcgg gagcagggcc gagcgcggct gggggtagcg ggcgatcagc
     3661 tcctcggccc gggtcagggt ctggtcggac aggggcacgg cggtggcggg ctcctttacg
     3721 ggatcagcgg tcgacgccgc cgaggaccgg gtcgatggag gacatgacgg cgatgatgtc
     3781 ggcgaccagc agcccgatcg ccatcggggt gccggtctgg aggttgacga acgacgggtc
     3841 gcggacgtgg acccggtagg gcaggttgcc gccgtcggag acgacgtggt agccgagctc
     3901 gccccggggc gactcgacgg gcacgtagac ctcgccccgc ggcaccttga tgccctcggt
     3961 gacctgcttg aagtgatgga tcagggcctc catggactcg cccatgatgt gctggacgtg
     4021 cgccggggag ttgcccagcc cgtcggggcc gagggcgagc ttggccggcc agccgacctt
     4081 ggggtcggtc gacatcaccg gcccgccggg caggctgtcc aggacctgct cgacgatccg
     4141 caggctctcg cgcatctcgg ccagccggac ctggtagcgg gcgtacacgt ccgaaccggt
     4201 ggccgtcggc acgtcgaact cgtactgctc gtagccgcag tacggctcgg ccttgcgcag
     4261 gtcgtggtcc aggccggcgg cccgcagcat cggcccggtg gtcccaaggc cgatcagctc
     4321 gtcgagggcg aggtggccga tgcccttggt ccgctccagc cagatcgggt tgttggtgag
     4381 cagggtctcg tactcgtcgg ccttgcttcg catgtcgcgg acgaaggcgc ggatcttgtc
     4441 ctcggtgccg tcgggcaggt cctgggagac cccggccggc cgcacccagg cgtggttcat
     4501 gcgcagcccg gtgacgtcct cgaggatgtc gaggatctcc tcccgctcc
//
LOCUS       DASSWR010000504         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_188644,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000504 DASSWR010000000
VERSION     DASSWR010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188644"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFO47_08475"
     CDS             complement(<1..193)
                     /locus_tag="VFO47_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788771.1"
                     /translation="MRHALVVTQLLHEARSAGPQAAERRELRLDLHGDLLATVGSSLC
                     PRPWPGKPRGGGRAERHTRP"
     gene            372..2147
                     /locus_tag="VFO47_08480"
     CDS             372..2147
                     /locus_tag="VFO47_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8788772.1"
                     /translation="MQVQPQLSALGVHDPAVLEPYLERRRYAGGERIMAQGTPGTECY
                     LITGGEVRLEVERPDFDSDGVIAYLGPGMLCGEFSLLDGQPRSASAYAHTEVTALVLS
                     EGGLGRLCADDPPTGLAVLRALGRDAAAKARETRGHLEEYVFAADIDPAVDAAVARAV
                     AAQAVLAGWPEARVEALLNAIAEAVAGQAGTLAAATVAETGIGNVADKTFKNRFASLE
                     VNRTLQGRPGIGPRPPDDRGVTEIASPVGVVLGLIPMTNPVATLVFKTLICVKARNAL
                     IVSHHPAAAGVGARTVTLIGEVLAAHGAPVALLQSVERASRTRTAMFMRHPQVALILA
                     TGGTAMVRAAYSSGTPAIGVGSGNVPALVCADADPVAAAGAVVESKAFDHGIICGSED
                     NLVVDAGIATPFLAALEAAGAAVCSEEEAARVAAAVFDPGDGHLRREALGQSAQAIAD
                     AAGVRRDQPIRVLVLPQPLEQVDGPLGREKLAPLLSLFRVPGTEEGLAICQRLLANQG
                     AGHTAVIHTGDQALQQRFAEEVPASRILVNGPATHGCIGVGNGLTPSLTLGCGTFGGN
                     STTDNVTYTNLLNIKRLARPFTGSG"
BASE COUNT          277 a          865 c          800 g          274 t
ORIGIN      
        1 ccggccgggt gtgacgttcg gcacgtcccc ctccccgggg cttgcccggc cacggccggg
       61 ggcacaagct cgacccgacc gttgcaagga ggtcgccatg caggtccagc cggagctcgc
      121 ggcgctcggc ggcctggggg ccggcggagc gggcctcgtg gagcagctgg gtgacgacga
      181 gagcgtgacg catggtggtc tcctcagtgg cggtgacgct cgcaaccctt gcctgctcag
      241 gatgaccgtc ggccccggcc gcggccgtgc cgccggtcac cgggcggatg tgacgtccgg
      301 cacccgccag gggcttgcct ccctccaccc ggaggcacaa gctcgacccg accgctccgg
      361 aggagggcag catgcaggtc cagccacagc tctcggccct cggggtgcac gacccggcgg
      421 tgctggagcc ctacctggag cgccgccgct acgccggcgg cgagcggatc atggcccagg
      481 ggactccggg caccgagtgc tacctgatca ccggcggcga ggtccgcctc gaggtggagc
      541 ggcccgactt cgactccgac ggggtcatcg cctacctggg gccgggcatg ctgtgcggcg
      601 agttcagcct gctcgacggc cagccccgct cggccagcgc ctacgcccac accgaggtga
      661 ccgccctggt cctgtccgag ggcggcctgg ggcggctctg cgccgacgac ccgccgaccg
      721 ggctggccgt gctccgggcc ctgggccgcg acgccgccgc caaggcccgg gagacccgcg
      781 gccatctcga ggagtacgtg ttcgcggccg acatcgaccc ggccgtcgac gcggccgtgg
      841 cccgggcggt ggccgcccag gcggtgctcg ccggctggcc cgaggcccgg gtcgaggcgc
      901 tgctgaacgc catcgccgag gcggtggccg gccaggccgg gacgctggcc gcggccaccg
      961 tggccgagac ggggatcggc aacgtcgccg acaagacctt caagaaccgc ttcgccagcc
     1021 tggaggtgaa ccggaccctc cagggccgcc ccgggatcgg cccccgcccg cccgacgacc
     1081 gcggcgtcac cgagatcgcc agcccggtcg gggtggtgct gggcctgatc cccatgacca
     1141 acccggtggc gaccctggtc ttcaagaccc tgatctgcgt caaggcccgc aacgccctga
     1201 tcgtcagcca ccacccggcc gccgccgggg tcggcgcccg caccgtgacc ctcatcgggg
     1261 aggtgctggc cgcccacggg gcgccggtgg cgctgctcca gtcggtcgag cgggccagcc
     1321 gcacccggac ggccatgttc atgcgccacc cccaggtggc gctgatcctg gccaccggcg
     1381 gcacggccat ggtccgggcc gcctacagct cgggcacccc ggccatcggc gttggctcgg
     1441 gcaacgtgcc cgccctggtc tgcgccgacg ccgacccggt ggcggcggcc ggcgcggtgg
     1501 tcgagagcaa ggccttcgac cacggcatca tctgcggctc cgaggacaac ctggtcgtcg
     1561 acgccggcat cgccaccccg ttcctggccg ccctggaggc ggccggggcg gcggtctgct
     1621 cggaggagga ggcggccagg gtggccgccg ccgtcttcga ccccggcgac gggcacctgc
     1681 ggcgcgaggc cctcggccag agcgcccagg cgatcgccga cgccgccggg gtccggcgcg
     1741 accagcccat ccgggtgctg gtcctgcccc agcccctgga gcaggtcgac ggccccctcg
     1801 gccgggagaa gctggccccg ctgctgtcgc tgttccgcgt ccccggcacc gaggaggggc
     1861 tggccatctg ccagcggctg ctggccaacc agggggccgg ccacacggcc gtgatccaca
     1921 ccggcgacca ggccctccag cagcgcttcg ccgaggaggt cccggccagc cgcatcctgg
     1981 tcaacgggcc ggccacccac ggctgcatcg gcgtcggcaa cggcctgacg ccgtcgctga
     2041 cccttggctg cggcaccttc ggcggcaact cgaccaccga caacgtcacc tacaccaacc
     2101 tgctcaacat caagcggctg gcccggccgt tcaccggatc cgggtgaccg cctgctggct
     2161 gttctccccg gtctcgccga tcaccacgtc gtcgggctcg cgctcggtga gctggt
//
LOCUS       DASSWR010000505         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_188735,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000505 DASSWR010000000
VERSION     DASSWR010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188735"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1566
                     /locus_tag="VFO47_08485"
     CDS             133..1566
                     /locus_tag="VFO47_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HET8788773.1"
                     /translation="MRIVIIGGGPGGYEAALVAADLGAEVTVVNDEGLGGACVLWDCV
                     PSKTLLTSAQAFNTLETAPSLGVHFEGMPGQEVPRYEVDLPRIMSRVRDLALAQSEDI
                     AKRVTAAGVRVVLGRGRIVDPHTVEVRPAGDGAAAGDGGRPERLEGDAILLATGSDPR
                     RMDTAPTDGEVILSSQDVYKLRKVPEHLVVVGAGATGAEYATAFLRFGAQVTFIVSRD
                     RILPTHDEDAALAIEDVFERRGLRTVKNARAAKVERVGNRGVVHLEDGRTIEGSHVLL
                     TIGQVPQTNGLGLEELGIQTDERGALVVDGVSRTSVPSIYAAGDVTAKLMLASVAAMQ
                     GRIAMWHLLGQAVSPLRWDAVVATIFTDPQIATVGLSEQRAAETGIEVEVDTLPLAGN
                     SRAKMSNRPDGFVKLLARPGSGTIAGGAVVASYASDLIAPISEACFNRLTASQLSQVF
                     QTYPSFAGSIQECARRLAVRVQGSYRY"
     gene            1777..>2623
                     /locus_tag="VFO47_08490"
     CDS             1777..>2623
                     /locus_tag="VFO47_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8788774.1"
                     /translation="MALLALTAAPGVTAQEQPAGKLTFTVGIINDVDSLNPFIGILAE
                     TYEVWALMYDQLVGYSQKDFSPVPGLAESWEVSDDELTWTYKIRQGVKWSDGRPLTAK
                     DAAYTFNRIMKGTFEQTNYGNYVSNIKTVEAPDDATLVMTTKKPSPSMLRLAVPILPE
                     HVWKDIDEKEVTTFDNEKDAVGSGPFVLAERSTGQFVRLTANKSYWGGAPKIDEVVYR
                     VFNNADAQLQALKKGEIDFADGLDPAPFNSLKDTEGITAVAGDYSGFDELAFNTGAAL
                     DTGEPI"
BASE COUNT          416 a          927 c          911 g          369 t
ORIGIN      
        1 gcggtcaatg gcggcctcgt gtcctcgtag tactgctcga tggccttgcg gatcaggtcg
       61 gagcgcggtc aacccgggcc gggccgtcca cagacccggt ttgaggcgct ggtgagtcgg
      121 taccatcgac ccatgcggat cgtgatcatt gggggaggac ctggcggcta cgaggccgcc
      181 ctggtcgcgg ccgacctggg ggcggaggtc acggtcgtca acgacgaggg gctgggcggg
      241 gcctgcgtgc tgtgggactg cgtgccgtcc aagaccctgc tgacctcggc ccaggcgttc
      301 aacaccctgg agacggcgcc cagcctcggg gtgcacttcg aggggatgcc gggccaggag
      361 gtgccccgct acgaggtcga cctgccgagg atcatgtccc gggtccgcga cctcgccctg
      421 gcccagtccg aggacatcgc caaacgggtc acggccgccg gggtgcgcgt ggtgctgggc
      481 cgggggcgga tcgtcgaccc ccacaccgtc gaggtccggc cggccggcga cggggccgcg
      541 gccggcgacg gggggcgccc cgagcggctg gagggcgacg ccatcctgct cgccaccggg
      601 tccgaccccc ggcggatgga caccgccccc accgacggcg aggtcatcct gtccagccag
      661 gacgtgtaca agctgcgcaa ggtccccgag cacctggtgg tcgtcggggc cggggccacc
      721 ggggccgagt acgccaccgc cttcctgcgg tttggcgccc aggtgacctt catcgtcagc
      781 cgcgaccgca tcctcccgac ccatgacgag gacgcggccc tggccatcga ggacgtgttc
      841 gagcgccggg ggctgcggac ggtcaagaac gcccgggcgg ccaaggtcga gcgggtcggg
      901 aaccgcgggg tcgtccatct ggaggacggc cgcaccatcg agggcagcca cgtgctgctc
      961 accattggcc aggtgcccca gaccaacggc cttggcctgg aggagctggg catccagacc
     1021 gatgagcgcg gcgcgctggt ggtggacggg gtcagccgca cctcggtgcc gagcatctac
     1081 gccgccggcg acgtcacggc caagctgatg ctggcctcgg tggcggccat gcagggccgg
     1141 atcgccatgt ggcacctgct cggccaggcc gtctccccgc tgcgctggga cgcggtggtg
     1201 gccaccatct tcaccgaccc ccagatcgcc accgtcgggc tgtcggagca gcgagcggcc
     1261 gagaccggca tcgaggtcga ggtcgacacg ctgcccctgg ccggcaactc gcgggccaag
     1321 atgagcaacc gacccgacgg cttcgtcaag ctgctggccc ggcccggctc gggcacgatc
     1381 gccggcgggg cggtggtggc cagctacgcc tcggacctga tcgcccccat ctccgaggcc
     1441 tgcttcaacc ggctcacggc cagccagctc tcgcaggtgt tccagaccta cccctcgttc
     1501 gccggctcga tccaggagtg cgcccgccgc ctcgccgtgc gggttcaggg gtcgtaccgt
     1561 tactagctgc ccaatccggc ccaccggccc ttgtcgaggc tctggcggca tcgcacaata
     1621 gcgcaactcc taattactga ctgttcagtc agctttgtcg gcggtggtgg gaatcccgca
     1681 agggggccgg gcgtgggcgc atccagcaga gcaagtcggt acgggcgggg ccgggggtcg
     1741 aaacggttcc ggagggcctc ggcgatcgcg gtgctggtgg cgctgctggc gctgaccgcg
     1801 gcgcccggcg tgacggccca ggagcagccg gccgggaagc tcaccttcac cgtcggcatc
     1861 atcaacgacg tcgactcgct caacccgttc atcggcatcc tggccgagac ctacgaggtc
     1921 tgggcgctga tgtacgacca gctggtcggc tacagccaga aggacttctc gcccgtgccc
     1981 ggcctggccg agtcctggga ggtctccgac gacgagctca cctggaccta caagatccgc
     2041 cagggggtga agtggtccga cggccggccg ctcacggcca aggacgccgc ctacaccttc
     2101 aaccggatca tgaagggcac cttcgagcag accaactacg gcaactacgt ctccaacatc
     2161 aagaccgtcg aggcccccga cgacgccacc ctggtgatga ccaccaagaa gcccagcccg
     2221 agcatgctgc ggctggccgt gccgatcctt cccgagcacg tctggaagga catcgacgag
     2281 aaagaggtca ccaccttcga caacgagaag gacgccgtcg gctcgggccc gttcgtgctc
     2341 gccgagcgca gcaccggcca gttcgtgcgc ctgacggcca acaagagcta ctggggcggg
     2401 gcgcccaaga tcgacgaggt cgtctaccgg gtcttcaaca acgccgacgc ccagctccag
     2461 gcgctcaaga agggtgagat cgacttcgcc gacgggctcg acccggcgcc cttcaactcc
     2521 ctcaaggaca ccgagggcat caccgccgtg gccggcgact actccggctt cgacgaactg
     2581 gccttcaaca ccggcgccgc cctggacacc ggcgagccga tcg
//
LOCUS       DASSWR010000506         5550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_101801,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000506 DASSWR010000000
VERSION     DASSWR010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101801"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /gene="ligD"
                     /locus_tag="VFO47_08495"
     CDS             complement(<1..1302)
                     /gene="ligD"
                     /locus_tag="VFO47_08495"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET8788775.1"
                     /translation="MATARRRRLPFPSALPASRAGDAWRLEADGRELRLSNLNKVYWP
                     GEGITKGDLLAYYWNVADLILPHLHDRPLTMKRMPNGLAGEPFYEKQVPAHAPDWLPT
                     AAVPTEEDSRVVEFVLAQDRASLLYVVNLGCIEMHPLHARAGSLDRPDYAFFDLDPFE
                     PYTYEDVRIVAKLVKVVLDGLGLRGYAKTSGATGMQVFVPLDGTHTHTDAREFVERVG
                     RLVVRAYPEKATMAWPVADRAGKVFIDHQMNRSGANIASVYSLRPLPGAPVSIPLDWD
                     ELDTDLEPGDFRIDNVWERFADGDRFAPVLDDKQSLTPAMEALGLTPGAPEEPRDRSR
                     PRFPPKRTTRPASRASLDEYVAKRDFTRTPEPPTPVAAPDLASPVAAPDPHPPVDGPP
                     NGRVARGGNGGERLSTTPAAPAAPTDPAAPTGPAASTGPTNP"
     gene            1428..2255
                     /locus_tag="VFO47_08500"
     CDS             1428..2255
                     /locus_tag="VFO47_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET8788776.1"
                     /translation="MKTIWKGAISFGLVTIPVRVYGATEAKSLRFNQLHEPDGGRIRY
                     KRVCSIDGEEVDYSEIVKGYEYEKDHYVTLTDEELDSLPVASAKAIEIERFVEAEEID
                     PIYFQKAYYLVPEGTGVKAYHLLREAMADDGKVALAKVAFRDKEHLATLRLRDNVFVL
                     ETMYWPDEIREPKFDVLDESVELRPQEIRMARSLIDSLTDAFQPEEFTDDYRTALEEL
                     VQKKIQGEEITYTEEAEPSKVVDLMEALKASVEAAKAGKPAPEPKTTSRRRKKAAGE"
     gene            2360..3238
                     /locus_tag="VFO47_08505"
     CDS             2360..3238
                     /locus_tag="VFO47_08505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET8788777.1"
                     /translation="MLDGLPARTGLGTWLLVRLRDDGGSVVLAGRDHTYGLRVGQELD
                     DADLPVRALIDGRGPRVARVAADVRAYAGGVPVGALAGVPVLAPDGTVFGCLCGLDPA
                     GGGPELAAELPTIELLGRLLAVLLVQELERQELQRRFELAELDALTDPLTGVGNRRAW
                     DRLLEAEEARCQRYGSVASLVAIDLDELKQVNDRQGHAAGDRLLCRTAQVIDSTRRAA
                     DVVARLGGDEFGVLAVECDEPAAKVLADRLRSALDAAGIRASVGHATRQPSGTLASAW
                     SAADAGMYTDKRRVRR"
     gene            complement(3235..4365)
                     /locus_tag="VFO47_08510"
     CDS             complement(3235..4365)
                     /locus_tag="VFO47_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET8788778.1"
                     /translation="MIRTRARLVTPVRRLVQLLLVAFVVIYFVLPQIAGARRALNLLA
                     GVNLWLIVLGVVLEAASILSYANLTRAMIHGTPPPYLTILRINLSTLAVSHVVPGGAA
                     VGGALGFRLLTRFGLSGTDAAFAMAAQGIGSAVVLNLLLWVGLLVSILSGQYNPLYAT
                     AALIGVLLLGGFSAVVVLLMRGERGAAGVMRAVTRRIPLLDEESVYRLVLRLASRLQT
                     LVADRRRLVRGLLWDLGFWLCSAASLWVFLLAFGYRAGIDGLIVAFGLAYVLAAIPIT
                     PAGLGVVEATMIALLTFFGADRGIATLGVVSYRLINFWLPIPLGALAYLSLQVEQETP
                     EGRRRIDERRKATELRRLAERSLRESEEYRVRLGRREPPPEG"
     gene            complement(4408..>5550)
                     /locus_tag="VFO47_08515"
     CDS             complement(4408..>5550)
                     /locus_tag="VFO47_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET8788779.1"
                     /translation="ARIEELVNIDSGSFTTAGVNRVADLCQARFEAGGWEVERHRHRP
                     DQEWAGAPLGDMVVGRRAGARPVADGGRRLLLLAHMDTVFDEGTAAARPFRTRDGRAY
                     GPGVTDDKAGIVCGFEAVEVLCDLAGFDDFAAITLVCSPDEEIGSPFSRPLIEALAGD
                     HDIAVGLEAARVNGALVSARKGISAFTVEVTGKAVHAGVRPTEGVNALLEAAHKTVAL
                     QGLNGRWDGVTCNVGVLRGGSRTNVVADRATMQVEVRAATTAAFDQAMDEVERIVAAT
                     TVSGARAELLPAHRHPPMERTEAVAALVAEAQAVARDLGFEVGEAATGGAGDANTTAA
                     AGLPTIDGLAPVGGEAHGPTEWLDLESVVPRTALLAGLLARLGANG"
BASE COUNT          778 a         1974 c         2096 g          702 t
ORIGIN      
        1 ggggttcgtg ggaccggtgg aggcggcggg gccggtgggc gccgcagggt cggtcggggc
       61 cgcgggtgcg gcgggggttg tggacagccg ttcgcccccg ttcccgccgc gggctaccct
      121 cccgttcggg ggcccgtcca caggtgggtg agggtccggc gcggccaccg gggaggcgag
      181 gtccggcgcg gccaccgggg tggggggttc cggggtgcgg gtgaagtcgc gtttggccac
      241 gtactcgtcg aggctggccc gggaggccgg gcgggtggtg cgcttggggg ggaacctcgg
      301 gcggctgcgg tcgcggggct cctcgggggc gccgggggtg aggccgaggg cttccatggc
      361 cggggtcagg gactgcttgt cgtccaggac cggcgcgaac cggtccccgt cggcgaaccg
      421 ctcccagacg ttgtcgatcc ggaagtcacc cggctcgagg tcggtgtcca gctcgtccca
      481 gtccagcggg atcgacaccg gcgccccggg cagcggccgc agcgagtaga cgctggcgat
      541 gttggccccc gaccggttca tctggtggtc gatgaacacc ttgcccgcgc ggtcggcgac
      601 cggccaggcc atggtcgcct tctccgggta ggcgcggacg accagccggc ccacccgctc
      661 cacgaactcg cgggcgtcgg tgtgggtgtg ggtcccgtcc aaggggacga acacctgcat
      721 gccggtcgcc cccgacgtct tggcgtagcc gcgcaggccg agcccgtcca ggacgacctt
      781 gaccagcttg gccacgatgc ggacgtcctc gtaggtgtac ggctcgaacg ggtccaggtc
      841 gaagaaggcg tagtcgggcc ggtcaaggga cccggcccgg gcgtgcaggg ggtgcatctc
      901 gatgcagccc aggttgacca cgtacagcag gctggcccgg tcctgggcga gcacgaactc
      961 gacgacccgg gagtcctcct cggtgggcac ggccgcggtg ggcagccagt cgggggcgtg
     1021 ggccggcacc tgcttctcgt agaacggctc gccggccagg ccgttcggca tccgcttcat
     1081 ggtcagcggc cggtcgtgga ggtgggggag gatcaggtcg gcgacgttcc agtagtaggc
     1141 cagcaggtcg cccttggtga tgccctcgcc cggccagtag accttgttca ggttggagag
     1201 gcgcagctca cggccgtcgg cctccagccg ccaggcgtcg ccggcccgcg aggcgggcaa
     1261 cgctgacgga aacggcaggc ggcggcgcct ggcggtggcc aacggtaccc gagcctcctt
     1321 ctgttgcgtt gcttgccagc gtacagttgt ccctggtccg tgaaccgtcg ccttccgacg
     1381 tgcccgccgc tcgcggagtg ttccgacgtc acaaggaggc ctggctcgtg aagacgatct
     1441 ggaagggggc gatctccttc gggttggtca ccatccccgt ccgggtgtat ggagccaccg
     1501 aggcgaagag cctccgcttc aaccagctgc acgagcccga cggcggccgg atccgctaca
     1561 agcgggtgtg ctcgatcgac ggcgaggagg tcgactactc cgagatcgtc aagggctacg
     1621 agtacgagaa ggaccactac gtcaccctga ccgacgagga gctggactcg ctcccggtgg
     1681 cctcggccaa ggccatcgag atcgaacggt tcgtcgaggc cgaggagatc gacccgatct
     1741 acttccagaa ggcctactac ctggtgcccg agggcaccgg ggtcaaggcc taccacctgc
     1801 tgcgcgaggc catggccgac gacggcaagg tcgccctggc caaggtcgcc ttccgcgaca
     1861 aggagcacct ggccaccctg cggctccgcg acaacgtgtt cgtgctggag acgatgtact
     1921 ggccggacga gatccgtgag cccaagttcg acgtcctgga cgagtcggtc gagctgcggc
     1981 cccaggagat ccgcatggcc cgcagcctga tcgacagcct caccgacgcc ttccagcccg
     2041 aggagttcac cgacgactac cggacggcgc tcgaggagct ggtccagaag aagatccagg
     2101 gtgaggagat cacctacacc gaagaggccg aaccttccaa ggtggtcgac ctgatggagg
     2161 cgctgaaggc gagcgtcgaa gcggccaagg ccggcaagcc ggcccccgag cccaagacga
     2221 ccagccggcg gcgcaagaag gcggcaggcg agtaggccag caggcgagga gccggcgtcc
     2281 cggtcccggg gcgtcgtggg gggtggacgt ggcgctcgac ctctgcccgt cgacggcctt
     2341 ccgggacgcg gtcgcatcca tgctggacgg actgccggca cggaccggcc tcggcacctg
     2401 gctgctggtg cggctccggg acgacggcgg gtcggtggtg ctggccggcc gcgaccacac
     2461 ctacggcctc cgggtcggcc aggagctgga cgacgccgac ctgcccgtcc gggccctgat
     2521 cgacggccgc ggcccccggg tggcccgggt cgccgccgac gtgcgggcgt acgccggcgg
     2581 cgtgcccgtc ggcgccctgg ccggggtccc cgtgctcgcc cccgacggca cggtgttcgg
     2641 ctgcctgtgc gggctcgacc cggccggcgg cgggcccgag ctggccgccg agctgcccac
     2701 catcgagctg ctggggcggc tgctggccgt cctgctggtc caggagctgg agcgccagga
     2761 gctgcagcgg cggttcgagc tggccgagct ggacgccctc accgacccgc tcaccggggt
     2821 cggcaaccgc cgcgcctggg accggctgct ggaggccgag gaggcccgct gccagcgcta
     2881 tgggtcggtg gcgtcgctgg tcgcgatcga cctggacgag ctcaagcagg tcaacgaccg
     2941 ccagggccac gccgccggcg accgcctgct gtgccgcacc gcccaggtga tcgactcgac
     3001 ccggcgggcc gccgacgtgg tcgcccgcct cggcggcgac gagttcggcg tcctcgccgt
     3061 cgagtgcgac gagccggccg ccaaggtcct ggccgaccgc ctccgcagcg ccctcgacgc
     3121 cgccggcatc cgcgcctccg tgggccacgc cacccgccag ccctccggca ccctcgcctc
     3181 tgcctggtcg gccgcggacg ccggcatgta cacggacaag cgacgggtcc gccgctagcc
     3241 ctccggcggg ggctcgcggc ggcccaggcg cacgcggtac tcctcggact cgcgcaggga
     3301 gcgctcggcc aggcggcgga gctcggtcgc cttgcggcgc tcgtcgatcc tccggcggcc
     3361 ctcgggagtc tcctgctcga cctggagcga caggtaggcc agagcgccga gggggatcgg
     3421 cagccagaag ttgatcaggc ggtaggagac gaccccgagg gtggcgatgc cccggtcggc
     3481 gccgaagaag gtgagcaggg cgatcatggt cgcctcgacc acgccgaggc cggccggggt
     3541 gatggggatg gcggccagca cgtaggccag cccgaaggcc acgatcagcc cgtcgatccc
     3601 cgcccggtac ccgaacgcga gcaggaacac ccacagcgag gcggccgagc agagccagaa
     3661 gccgaggtcc cagagcaggc cacggaccag ccgccggcgg tcggcgacca gcgtctggag
     3721 ccgcgacgcc agccggagga ccaggcggta gacgctctcc tcgtccagga gcgggatccg
     3781 ccgggtcacc gcccgcatca ccccggcggc gccccgctcg ccccgcatca gcagcaccac
     3841 caccgccgag aagccgccca gcagcagcac cccgatcagg gcggcggtgg cgtacagggg
     3901 gttgtactgg ccgctcaaga tcgacaccag caggcccacc cagagcagga ggttcagcac
     3961 caccgcggag ccgatgccct gggcggccat ggcgaaggcg gcgtcggtgc cggacagccc
     4021 gaagcgggtc agcagccgga acccgagcgc cccgccgacg gccgcgccgc ccgggacgac
     4081 gtggctgacg gccagggtcg acaggttgat ccggaggatc gtcaggtagg ggggcggggt
     4141 gccgtggatc atcgcccggg tcaggttggc gtagctgagg atcgaggccg cctccaggac
     4201 gaccccgagg acgatcagcc acaggttgac cccggccagc aggttcaggg cccggcgcgc
     4261 cccggcgatc tgggggagca cgaaatagat caccacgaag gcgaccagca ggagctggac
     4321 gaggcgcctg accggcgtga ccaaccgcgc tcgggtccgg atcatgggtg ctgccagtat
     4381 aaatggcgac ctgcggaccc ccggggacta cccgttggcc cccaggcggg ccagcagccc
     4441 ggccaggagg gcggtgcggg ggacgacgct ctcgagatcg agccactccg tcggcccgtg
     4501 ggcctcgccg cccaccgggg ccaggccgtc gatcgtgggc aggccggcgg cggcggtggt
     4561 gttggcgtcc ccggccccgc cggtggccgc ctcgccgacc tcgaacccga ggtcgcgggc
     4621 gacggcctgg gcctcggcga ccagcgcggc cacggcctcg gtgcgctcca tcggcgggtg
     4681 gcggtgggcg ggcagcagct cggcccgggc gccggagacc gtggtcgcgg ccacgatccg
     4741 ctcgacctcg tccatggcct ggtcgaaggc ggccgttgtc gccgccctga cctcgacctg
     4801 catggtcgcc cggtcggcga ccacgttggt ccggctgccg ccccggagca cgccgacgtt
     4861 gcaggtcacc ccgtcccagc ggccgttcag gccctgcagg gcgaccgtct tgtgggccgc
     4921 ctccaggagg gcgttgaccc cctcggtggg gcggaccccg gcgtggacgg ccttgccggt
     4981 gacctccacg gtgaacgccg agatgccctt gcgggccgag accagggcgc cgttgacccg
     5041 ggccgcttcc agccccacgg cgatgtcgtg gtcgccggcc agggcctcga tcagcggccg
     5101 gctgaacggc gagccgatct cctcgtcggg cgagcagacg agcgtgatgg cggcgaagtc
     5161 gtcgaacccg gccaggtcgc acaggacctc gacggcctcg aacccgcaca cgatcccggc
     5221 cttgtcgtcg gtcacgcccg gcccgtaggc gcgcccgtcc cgggtccgga acggccgggc
     5281 ggcggccgtc ccctcgtcga acaccgtgtc catgtgggcc agcagcagca ggcgccgccc
     5341 gccgtcggcc accggccggg ccccggcccg ccggccgacc accatgtcgc ccaggggggc
     5401 gccggcccac tcctggtccg ggcggtggcg gtggcgctcc acctcccagc cgccggcctc
     5461 gaagcgggcc tggcacaggt cggcgacccg gttgacgccg gcggtcgtga aggagccgga
     5521 gtcgatgttg accagctcct cgatccgggc
//
LOCUS       DASSWR010000507         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_161004,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000507 DASSWR010000000
VERSION     DASSWR010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161004"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFO47_08520"
     CDS             complement(<1..245)
                     /locus_tag="VFO47_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET8788780.1"
                     /translation="MDEGEWLVERFQTHRAHLHAVAYRMLGSLSEADDAVQEAWLRLS
                     RAGTGGVDDLGRWLTTVVARVCLDMLRSRAARREEPLG"
     gene            321..818
                     /locus_tag="VFO47_08525"
     CDS             321..818
                     /locus_tag="VFO47_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8788781.1"
                     /translation="MPPIVSHIDIACPPAEVFGYATDPSRFAEWQDDVVGVRIQGGRP
                     LGVGSRFTTTRRVARVEFSYTQEITVLNPPRSWAARGVDGPLRPSASITVEPLEGGIW
                     SRVTFTLEFQGKGIGKLVPDVVRQLALKGAPKSYRHLKERLERGDWRAAGRAGAMPPA
                     NGRAV"
     gene            1045..>2494
                     /locus_tag="VFO47_08530"
     CDS             1045..>2494
                     /locus_tag="VFO47_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET8788782.1"
                     /translation="MAGTAADRVLVNRYALKTPLGRGGMGVVWRAHDVVLGRDVAVKE
                     VVFPASLSAAERAAAQARVTREARAAARLNHPGVVTLYDAVTSQGSVFLVMELVEAPT
                     LAELVRVEGPLAPERVAEIGAQLAGALEAAHHAGIIHRDVKPANVLVPPSGNAKLADF
                     GVASLADDPRLTTTGLVIGSPAYMAPEQAKGEATGPPADFWALGATLYFAVEGEPPFD
                     KGSSMAALAAVVNEAPQPMRRAGPLAPLLAALLAKDPQARPSGPKVRAWLAWLLVVTP
                     STPPSQIPPTRRPGPSRLLASPPSTSEAPRTSASSSVTAGAGLPQRSEEVPAVEQDQA
                     PATEPPAGPAPVQRSPRPILPPAPPVPHWARRRLPGVAALLLVGGMLTAWLTGTFQPD
                     PTRDRPAPAITSRSPGVASGEDQATPAPARGGAGAPGTTEPAPSTTKEPATTRAPTTP
                     TTAAPKTGVPAGWRAFTNRAGNNRAGVPPGFRA"
BASE COUNT          351 a          905 c          901 g          337 t
ORIGIN      
        1 cccaggggct cctcgcgacg ggcggcgcgc gagcgcagca tgtccagaca cacccgcgcg
       61 accaccgtgg tcagccaccg gccgaggtcg tccaccccgc cggtgccagc gcggctgagg
      121 cgcagccagg cttcctggac ggcgtcgtcg gcctcgctga gcgagcccag catccggtag
      181 gccaccgcgt gcaggtgggc acggtgggtc tggaagcgct cgaccagcca ctcgccctca
      241 tccatcggtc acattccctc gtgttggttc gtcatcacag tgacgggtca acacgagccg
      301 atgtgacaag gagggcagcc atgccgccga tcgtgtccca catcgacatc gcctgcccgc
      361 cggcggaggt cttcgggtat gccactgatc cttcgcggtt cgccgagtgg caggacgacg
      421 tggtgggcgt gcgcatccag ggaggccgtc cgctcggggt gggctcgagg ttcaccacga
      481 cccgccgggt cgcccgtgtc gaattcagct acacgcagga gatcacggtg ctcaatccgc
      541 ccagaagctg ggccgctcgc ggtgtcgatg gaccgctcag gcctagcgcg agcatcaccg
      601 tcgagccgct tgaggggggc atctggtccc gggtcacgtt cacgctcgag ttccagggaa
      661 aggggatcgg caagctggtg ccggacgtcg tccgccagct ggcgttgaag ggagcgccca
      721 agagctaccg gcacctgaag gaacggctgg aacgtggcga ctggcgcgca gccggccggg
      781 caggagcgat gcctccggcg aacgggaggg ccgtgtaggc agccaggctg accagggtgc
      841 tattgggtga cgcggcgtca gcggtggagg gccagggaga tgacccgcag caggctggcg
      901 gtgccggcgg tctcgggtcg cgcaccggct ggcgccgccc cagcccgggt cgtgctgatc
      961 taggcgccgt ccatgccctc ggcgggtgag ggattcggcg tgactgccgg gttcctacct
     1021 gtcccggtgg gtaggcggga cgagatggct ggaaccgccg cggatcgcgt cctcgtcaac
     1081 cgctatgcgc tcaagacccc gctggggcga ggcgggatgg gggtggtgtg gcgtgcccac
     1141 gatgtggtgc tgggacgcga tgtcgcggtc aaggaggtgg tgttcccggc gagcctgtcg
     1201 gctgcggagc gggccgcggc ccaagcgcgg gtgacccggg aagcgcgggc agcggcccgg
     1261 ctcaaccacc cgggggtggt gaccctctac gacgcggtga cctctcaggg cagcgtcttc
     1321 ctcgttatgg agctggtgga agcacccacc ctggccgaac tggtccgggt cgagggtccg
     1381 ctggcgcccg agcgagtggc ggagatcggc gcccagctcg ccggcgcgct ggaggcggcg
     1441 catcacgccg gcatcatcca tcgcgacgtc aaacccgcca acgtgctggt gcccccctca
     1501 gggaatgcca agctggccga cttcggtgtc gcctcgctag cggacgaccc gcggctgacc
     1561 acgaccggac tggtcatcgg ctcacccgcc tacatggccc ccgaacaggc caagggtgag
     1621 gcgaccgggc cgccggcaga cttctgggcg ctgggggcga cgctgtactt cgcggtcgag
     1681 ggcgagccgc cgttcgacaa gggctcttcg atggcggccc tggccgcggt ggtcaacgag
     1741 gcgccgcagc cgatgcgccg ggccgggccg ctggccccgc tgctggccgc gttgttggcc
     1801 aaggacccgc aggctcgccc gtccggtccg aaggtccggg cctggctggc ctggctcctg
     1861 gtcgtgacac cttcaactcc acccagccag atccccccga cccgaagacc gggtcccagc
     1921 cgcctcctcg cgtcgcctcc gtccacctcc gaggccccga ggacgtcggc ctcctcctcc
     1981 gtcacggcgg gcgctggcct cccacagcgt tccgaggagg tcccagcagt cgagcaggac
     2041 caggcgccgg cgacggagcc tccggcggga ccggcaccgg tccagcggtc accccgcccg
     2101 atccttccgc cggccccacc cgtgccccac tgggcgcggc ggcggctgcc cggcgtggct
     2161 gccctgctgc ttgtgggcgg gatgctgacc gcctggctga cagggacctt ccagcccgac
     2221 ccgacccggg accggccggc tccggccatc accagccgaa gcccgggggt ggcctcaggc
     2281 gaggatcagg cgaccccggc cccggccagg ggcggcgccg gggcaccagg cacgaccgag
     2341 cccgctccat cgaccaccaa ggagcctgcc accaccagag cgcccacgac ccctaccact
     2401 gccgcaccca agaccggcgt tccggccggt tggcgcgcct tcaccaaccg ggccggcaac
     2461 aaccgcgccg gcgtcccccc cgggttccgg gccc
//
LOCUS       DASSWR010000508         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_612427,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000508 DASSWR010000000
VERSION     DASSWR010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612427"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFO47_08535"
     CDS             complement(<1..1289)
                     /locus_tag="VFO47_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018720244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788783.1"
                     /translation="MVLPLEGLVAAYGVACPFQAQGAYLRTRGLGPDFARLWTANAVS
                     NLGDGITLVAGPLLAATLTRDPRLVAGLAVAQRLPWLLFSLVSGALVDRLDRRLLMVR
                     VDAARCLAVGLLGVAVLADVASLPLLYVVFFALGTAETLFDNAAVSILPAVVPRDQLA
                     RANSRLLGARMVANELVAPPLGGLLFAAAAAVPFLLDAGTFAAAAALVAAMGGRFRVE
                     RPEGAAPTTLRAEIAEGVRWLARNRLLRILAVAIALMNLTLSATLSLSVLYAQERLGL
                     GSVGYGLLLSSMAVGGIAASLVADRVIGRLGPATTMRLGLVIESSTHLVLALARSPIL
                     VGVMFALFGFHAMTWSVISVSLRQELIPARLLGRVNSAYALFGFGSLSLGAVAGGVLA
                     ARYGLTAPFWCSFAAMTVLTVTCWPILSARAIARARA"
     gene            1329..2084
                     /locus_tag="VFO47_08540"
     CDS             1329..2084
                     /locus_tag="VFO47_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET8788784.1"
                     /translation="MNRVLPLLLVIAALTAAGCDAAGSGASTGAAPPATTSPGVTAAP
                     GGTAVKGGSTLGAQAAPRFRGHVVAGREAVRSRVRYSWRRGCPVGPVELRLLRVDHWG
                     LDGRVHRGELIVHRDHARRILAVMGKLFKARYPIQRLRLVDAYRASDDRSMAANNTSA
                     FNCRRVSGSSRWSEHAFGRAIDVNPLRNPYVTSGGRVSPPAGRPYANRARRAAGMIHG
                     GDAVVRAFAAAGWRWGGYWSGSKDYQHFSSTGR"
     gene            2191..2415
                     /locus_tag="VFO47_08545"
     CDS             2191..2415
                     /locus_tag="VFO47_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HET8788785.1"
                     /translation="MSSTETRPVESDADTGEDDGHAHYARKDEITRAAIEGGTVVALC
                     GRRFPPIRDPQRFPVCPKCKELAAMLWGPE"
BASE COUNT          382 a          921 c          977 g          295 t
ORIGIN      
        1 tgggcgcggg ccctggcgat ggcccgggcg ctcaggatcg gccagcaggt cacggtgagc
       61 acggtcatgg cggcgaagct gcaccagaag ggcgcggtca gcccgtagcg ggcggccagc
      121 acgccgccgg cgacggcacc cagggacagc gagccgaagc cgaacagcgc gtaggcgctg
      181 ttgacccggc ccagcagccg ggccgggatc agctcctggc gcagcgacac gctgatcacg
      241 ctccaggtca tggcgtggaa gccgaacagc gcgaacatga ccccgaccag gatcgggctg
      301 cgggccaggg ccagcaccag gtgggtggac gactcgatga cgaggcccag acgcatggtg
      361 gtggccgggc cgagccggcc gatgacccgg tcggcgacca ggctggcggc gatgccgcca
      421 accgccatcg acgacagcag caggccgtag ccgaccgagc cgagcccgag ccgctcctgg
      481 gcgtagagaa ccgacaggga cagggtcgcc gacagggtca ggttcatcag cgcgatggcc
      541 acggccagga tgcgcagcag ccggttcctg gccagccagc ggaccccttc ggcgatctcg
      601 gcgcggagag tggtcggggc ggcgccctcc ggccgctcga cccggaaccg gccgcccatg
      661 gccgcgacca gggcggccgc ggccgcgaag gtcccggcgt cgagcaggaa cggcacggcg
      721 gcggcggccg cgaacagcag cccgccgagc ggcggggcga ccagctcgtt ggccaccatg
      781 cgcgccccca ggaggcggct gttggcccgg gccagctggt cccgggggac gacggcgggc
      841 aggatcgaga cggccgcgtt gtcgaacagg gtctcggcgg tgccgagggc gaagaacacc
      901 acgtacagca gcggcaggct ggccacgtcg gcgagcacgg ccacgccgag cagcccgacg
      961 gccaggcagc gggcggcgtc gacccgcacc atcagcaggc ggcggtccag ccggtcgacc
     1021 agtgccccgc tgacaagtga gaacagcagc caggggaggc gctgggcaac ggccaggccg
     1081 gccaccagcc gcgggtcgcg ggtcagggtc gcggccagca ggggccccgc gaccagggtg
     1141 atgccgtcgc ccaggttgga cacggcgttc gccgtccaca atctggcgaa gtcgggcccg
     1201 aggccgcgcg tgcgcaggta cgctccttgt gcctggaagg gacaggccac accatacgcc
     1261 gccaccaggc cctcaagcgg gaggaccatc gggacgacac atcaccatat gaagcgagcc
     1321 ctgccgcgat gaaccgggtc ctgccgctgc tgctggtcat cgccgccctc accgcggccg
     1381 gctgcgacgc ggccgggtcc ggcgcgtcca ccggcgccgc gcctcccgcc accacctccc
     1441 cgggggtcac cgccgccccc ggcgggacgg ccgtgaaggg tgggtccacg ctgggggcgc
     1501 aggccgcgcc gaggttccgt gggcacgtcg tggccgggcg ggaggcggtg cggtcgcggg
     1561 tgcggtactc gtggcggcgg gggtgcccgg tggggccggt ggagctgcgg ctgctgcggg
     1621 tcgaccactg ggggctggac gggcgggtcc accggggcga gctgatcgtg caccgcgacc
     1681 acgcccggcg gatcctggcc gtgatgggga agctgttcaa ggctcgctac ccgatccagc
     1741 gcctgcgcct ggtcgacgcc taccgggcca gcgacgaccg gtcgatggcc gccaacaaca
     1801 cctcggcgtt caactgccgc cgggtgtcgg ggtccagccg ctggtcggag cacgccttcg
     1861 gccgcgcgat cgacgtcaac ccgctgcgca acccgtacgt gacctccggc ggccgggtgt
     1921 cgcccccggc cggccggccc tacgccaacc gggcccggcg ggccgccggc atgatccatg
     1981 gcggcgacgc ggtcgtgcgg gccttcgccg ccgccggctg gcgctggggc ggctactggt
     2041 ccggctccaa ggactaccag cacttctcct ccaccggccg ctgagccacg ccatcatgcg
     2101 cgctcgaagc ggttcgcggc ccgggggtgc gggggcgggc ggcttcggcg cagaatggcg
     2161 ggcgccagcg gactgtggga ggagccgacg atgtccagca ccgagacccg accggtcgag
     2221 tccgacgccg acaccggcga ggacgacggg cacgcccact acgcccgcaa ggacgagatc
     2281 acgcgtgccg cgatcgaagg tggaaccgtc gtcgcacttt gtggtcgcag attccccccg
     2341 attcgcgatc cccaacgctt tccggtctgt ccaaagtgca aggaattggc cgccatgttg
     2401 tggggccctg agtagtcggg gacgagcccg gccgacgtcc ggccgcccca gtcgcgcctg
     2461 ggtattcggc gtcctcttcg gcggtggact ccggcatcta ggcttcgttc tgccaggact
     2521 acgccactac cgactgcggc cgccagctgt ggcgttccgg gcctatgtcc gcgac
//
LOCUS       DASSWR010000509         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_922916,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000509 DASSWR010000000
VERSION     DASSWR010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922916"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..133
                     /locus_tag="VFO47_08550"
                     /pseudo
     CDS             1..133
                     /locus_tag="VFO47_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(462..1319)
                     /locus_tag="VFO47_08555"
     CDS             complement(462..1319)
                     /locus_tag="VFO47_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8788786.1"
                     /translation="MLTKEDEIELSQAYEAGLDAQLKLADTAPDDPARPDLEAVAERG
                     EWARRKMIESNLRLVVSIARRFSATGLPLGDLVQEGNLGLLRAVEKFDWRKGFKFSTY
                     ATWWIRQAIARGAADRGARAIRLPVHVDEQVGRLRRTQTRMHELLGREPTDDELAAEL
                     DMPTEKVNRLKDTAQAITSLDTPIGDDGAALQDFLEDDSAVGPDELAVEAVGREALEQ
                     VLNALPERERQVLILRFGLDSGTPRTLEEVGAVMGFSRERARQVERDALASLRSPEIR
                     ARLEDLVAA"
     gene            complement(1644..1952)
                     /locus_tag="VFO47_08560"
     CDS             complement(1644..1952)
                     /locus_tag="VFO47_08560"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET8788787.1"
                     /translation="MATSGFSEAVWALARRVPAGQATTYGLLAEAHYGVAKGARGVGQ
                     AMARCPGDVPWWRVVHADGAMKGTPGADEQRARLAEEGVPLTRDGRVDWAQAGGPWSP
                     "
BASE COUNT          289 a          726 c          716 g          272 t
ORIGIN      
        1 cagggagcgc gaggtgctgg gcctgatggc cgagggacgc tccaacgccg gcatcgcccg
       61 ccggctgtgg gtgaccgagg gcaccgtgga gaagcacgtg cgcagcatcc tgaccaagct
      121 gacgcttccc gaggccaccg cggcggccag cacctcgggg tcgttcaggt cgcggccctg
      181 gtcgtggcgg gcggcgaaca gggccaggtg cacggccggg aaccgctcgg gcagctcgtc
      241 gcggacggcc aggccccact ggagggccag cacgcccgag cgcggctcgg cgcccggctg
      301 ccagaccggc ggctcaccat cgtccaggtg gacctgggag agggagaagg cgcggaagcc
      361 gacctcggcc ggctcccctt cctccagcgc gcgcacgacc acctccgccg cgttccgtgc
      421 gaacgggcag aggtagtcgt gggtgacggc gaagcggcgc atcaggccgc gacgaggtcc
      481 tccaggcggg cgcggatctc ggggctgcgc agggaggcca gggcgtcgcg ttcgacctgg
      541 cgggctcgtt cccgggagaa ccccatgacc gccccgacct cctccagcgt ccgcggggtg
      601 cccgagtcca gaccgaagcg caagatcaac acctggcgtt cgcgttcggg cagggcgttg
      661 aggacctgct ccagggcctc gcggccgacc gcctccacgg ccagctcatc cgggccgacc
      721 gcggagtcgt cctccaggaa gtcctggagg gcggccccgt cgtccccgat cggggtgtcc
      781 aggctggtga tggcctgggc ggtgtccttg agccggttca ccttctcggt cggcatgtcg
      841 agctcggccg ccagctcgtc gtcggtcggc tcgcggccca ggagttcgtg catgcgggtc
      901 tgggtgcgac gcaaccggcc cacctgctca tcgacgtgga cggggaggcg gatggcccgg
      961 gcgccccggt cggcggcccc gcgggcgatg gcctggcgga tccaccaggt ggcataggtg
     1021 gagaacttga accccttgcg ccagtcgaac ttctccaccg cccgcaacag gcccaggttg
     1081 ccttcctgga ccaggtcacc caggggcagg ccggtggctg agaagcggcg ggcgatggag
     1141 acgaccaggc gcaggttgga ctcgatcatc ttgcggcggg cccactcgcc ccgctcggcg
     1201 accgcttcca gatcggggcg ggcggggtcg tcaggggcgg tgtcggcgag cttgagctgg
     1261 gcgtccaggc cggcctcata ggcctgggac agctcgatct cgtcctcctt ggtcaacaac
     1321 gggtgccgac cgacctaatc aagatagaga cggaacgagt ccgtcgtctg gaggtcgagc
     1381 agctggtcct ggtccttcat cgcactcctt gcgacagcac gcgatcatcg ggggtacaca
     1441 cgtcactctc agttttgcca acaaccatgt tcgcaaaacc tattcccgtc taccaggggt
     1501 tctcggaaga aactgcatcc gaggtgaggg gcagaggccg ggagtgcctc aacaccgcca
     1561 acggcgacca taccacggcc cagccggcaa cgtgtcactt cccaccggcc accggggtcg
     1621 ggcaaccgcg ccgacgccag cggtcagggc gaccaggggc ccccggcctg cgcccagtcg
     1681 acccggccgt cacgggtcag gggcacgccc tcctcggcca gccgggcccg ctgctcgtcg
     1741 gcccccgggg tccccttcat cgccccgtcg gcgtggacga cccgccacca gggcacgtcc
     1801 cccgggcagc gggccatcgc ctggccgacc cccctggccc ccttggccac cccgtagtgg
     1861 gcctcggcga gcaggccgta ggtggtggcc tggccggccg gcacccgccg ggccagcgcc
     1921 cacaccgcct ccgagaagcc gctcgtggcc accgcctacc tcccgggcgg gtcctgggcg
     1981 tcggccaggt actcgtggac cag
//
LOCUS       DASSWR010000510         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_330420,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000510 DASSWR010000000
VERSION     DASSWR010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330420"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFO47_08565"
     CDS             <1..271
                     /locus_tag="VFO47_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788788.1"
                     /translation="ILLEETALPERPLMLTVVTWTVALSVYAHGVTAWPGANRYADWY
                     AAHRSDHASMPESVPATEQRTRAPALHTQRLGPEHRATRSLPKGP"
     gene            complement(412..>2472)
                     /locus_tag="VFO47_08570"
     CDS             complement(412..>2472)
                     /locus_tag="VFO47_08570"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET8788789.1"
                     /translation="ASRKGGGVGWVTLDEHDNDPAVMLTYLAVALDRIEPIDPGVFRA
                     LAAPGASVLGTIVPRLITAVAAMTRPVALVTDNLEVLDNPQCLDAMAEVVARLPTGLQ
                     LALAARTTPRLPTALVGTRGQVMAVGVEELRMDQDEAHALLEGAGVQLGDAETAALVG
                     LTEGWPVGLYLAALARKARRADGPQEETGTEFSGDYRLVADYLWAELLSHQPQSTVSF
                     LTRTAVLDRMCGPLCDAVLGTTRSADRLASLARSNLLLVPLDQQQQWYRYHHLFRGLL
                     QAELDRREPELVQELHLRAAEWYEANGLPETAIDHAQAAGDADRVARLVASLALPTGS
                     TGRIDTVHRWLAWFEDRGLIERYPLVAVQGIWVYALWGQAAAADRWFDAAERGSFAGT
                     LPDGSTVAGYRSRLAALLCRDGVDRMRADAEAALAGLSPGSPWRAAALLLQGMSYLLV
                     GEVDQADPILARAVEVGTQVGYLPALSIALAERCLVAIERRDWQQAESLADRARTIVE
                     HGRLDHYIPISLVYAVAARTSIHRGATQTAQEHLVQAARLRPQLTYATPHLAVQTLLE
                     LGRAYLGLGDAAGGRVTLTEANDILHRRPDLGLLSSQADELGSRLDRQHEPIMGASSL
                     TTAELRLLPLLPTHLSFREIGERLYISQHTAKKHATTLYQKLGVSSRSQAVQRARQLG
                     LLGK"
BASE COUNT          383 a          877 c          845 g          367 t
ORIGIN      
        1 gatcctgctc gaggagaccg cacttcccga gcggcccctc atgctgaccg tggtcacctg
       61 gacggtggcg ctgtcggtgt acgcccacgg cgtgaccgcc tggccgggcg ccaaccgcta
      121 cgccgactgg tacgccgccc accgtagcga ccatgcttcg atgccggaaa gcgtgccggc
      181 caccgaacaa cgcacccgcg cccctgcact ccacacgcag cgcctagggc ctgaacatcg
      241 ggccacccga tctctgccga aaggcccctg atcagctgga cgatccgaca cggtggcgcc
      301 tgtccgtggc tgggctggga ttccagagcg acctcgtgga cgtcgcagta cgagcggctg
      361 gccgcgcgcc gtcgatcgct tccggcttcg cagcaaaggt ccaggcgaga cctacttgcc
      421 gagcaggccg agctgacggg cgcgctggac cgcctggctg cgggaggaca cgccgagctt
      481 ctggtacagc gttgtcgcgt gtttcttggc cgtgtgctgt gagatgtaca gccgctcgcc
      541 gatctctcga aagctgaggt gggtgggcag cagggggagc aggcgcagct cagcggtggt
      601 gagcgacgac gcgcccatga tcggctcatg ttgtctgtcc agcctggacc ccagctcgtc
      661 ggcctggctg gacaggaggc cgaggtcggg tcgccggtgg aggatgtcgt tggcctcggt
      721 cagggtcacc ctgccaccgg cggcgtcgcc cagccccagg taggcgcggc cgagttccag
      781 cagcgtctgg acggcgaggt gcggcgtcgc gtaggtgagc tgcgggcgca ggcgggcggc
      841 ttggacgagg tgctcctggg ccgtctgggt cgcaccccga tggatcgatg tcctggccgc
      901 gaccgcgtag accaggctga tgggaatgta gtggtccaga cggccgtgct ccacaatcgt
      961 gcgcgcccgg tccgccaagc tctcggcctg ctgccagtca cggcgttcga tcgcaaccag
     1021 gcagcgctcg gccagggcaa tcgacagggc ggggagataa ccgacttggg tgcccacctc
     1081 gaccgcacgg gccaggatcg ggtcggcctg gtcgacctcg ccgacgagca ggtaggacat
     1141 cccttggagg agcagcgccg cggcccgcca agggcttcct gggctcagcc cggccagggc
     1201 ggcctccgcg tcggcgcgca tccgatccac cccgtcgcgg cacaacagcg cggccaaccg
     1261 cgaccggtag cccgccaccg tgctgccgtc ggggagcgtc ccggcgaacg acccacgttc
     1321 ggccgcatcg aaccaacggt cggcggccgc cgcctggccc cacaacgcgt agacccagat
     1381 cccctgcacg gcaaccagcg gatagcgttc gatcaaccca cgatcctcga accatgccaa
     1441 ccaccggtgg acggtatcga tccgtcctgt ggagccggtg ggcagggcaa ggctggcgac
     1501 cagccgggcg acccggtcgg cgtcccctgc ggcctgggca tggtcaatcg cggtctccgg
     1561 gaggccgttg gcctcgtacc actccgccgc ccgtaagtga agctcctgaa ccaactccgg
     1621 ctcgcgccgg tcgagttcgg cctggagcag accgcggaac aaatggtggt agcggtacca
     1681 ctgttgttgc tggtccaggg ggaccaacaa caggttcgac ctggccagcg acgccaaccg
     1741 gtcggccgag cgggtggtgc ccaggaccgc gtcgcacagc ggcccgcaca tccggtcaag
     1801 caccgccgtg cgggtcagga acgacaccgt cgactgcggc tggtgggaca acaactcggc
     1861 ccacaggtag tcagccacga gccggtagtc ccccgaaaac tcggtcccag tctcctcctg
     1921 cgggccgtct gctcgtctcg ccttgcgggc cagcgcggca agatacagtc ccaccggcca
     1981 cccttccgtc agtccgacca gcgccgccgt ctcggcgtcg cccagttgga ccccggctcc
     2041 ctccagcagg gcatgcgcct catcctggtc catcctgagc tcctcgaccc cgactgccat
     2101 gacttggcct cgtgtgccca ccagcgccgt cggcagcctg ggggtggtcc gggcagccag
     2161 cgccagctgc aacccggtgg gcaggcgcgc gaccacttcg gccatggcat ccaggcactg
     2221 cggattgtcc agcacctcca gattgtcggt gaccagggca accggccggg tcatcgctgc
     2281 cacagcggtg atcagccggg gcacgatcgt gcctaggacc gaggcgcctg gtgcggccag
     2341 ggctcggaag acgcccgggt cgatcggctc gatgcggtct agggcgacgg ctaggtacgt
     2401 cagcatgacc gcagggtcgt tgtcgtgctc gtcgagcgtc acccagccca cgccgccacc
     2461 tttgcggctg gc
//
LOCUS       DASSWR010000511         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_556800,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000511 DASSWR010000000
VERSION     DASSWR010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556800"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..583
                     /locus_tag="VFO47_08575"
     CDS             194..583
                     /locus_tag="VFO47_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8788790.1"
                     /translation="MPSNRVLVVEDDPSVRGLLQTILEDEALEVILAADGEEGLRLAR
                     TVDPDVVLLDVMMPGLGGPDVIRRLRRADGSLPFAVLVVTGAAEVIAPLRSELGPEAV
                     LEKPFDITTLATRVKSLASGNASGERP"
     gene            580..1512
                     /gene="argF"
                     /locus_tag="VFO47_08580"
     CDS             580..1512
                     /gene="argF"
                     /locus_tag="VFO47_08580"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET8788791.1"
                     /translation="MSKRDFLSIDNVEPGELTAILDGADGHKADRRRRDTLGGRSVAL
                     VFEKPSTRTRVSFEVAVHELGGYPLPLAGAELQLGRGETLEDTAAVLSRYVHAIVLRT
                     FAQESLERLARGGTIPVVNALSDYSHPCQALADLQTIRERKGSLAGIRLAYLGDGNNV
                     AHSLLFAGAKMGMHVAVATPVGYEPIPQVVRRATEIAATTGGGVELTHDPSTAAKDAD
                     VLYTDVWASMGKEMEHGERALIFKPYQLSTDTVTAAAPDVMVLHCLPAHRGEEIAAEV
                     MDGPNSAVFAQAENRLHTQKALLSWLLTGSTDPS"
     gene            1517..2068
                     /locus_tag="VFO47_08585"
     CDS             1517..2068
                     /locus_tag="VFO47_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788792.1"
                     /translation="MTEPDDHGGRPGRSPGRSAWRWAVAAGLAGVAASILTTALAAAV
                     AGRESDLALQPVELALRVGLLLIAPCVGAILAAVVVPVRSEHGGRLAALAVGLAVPLG
                     PLLVLRLDRMREFGLASAALTVGVMAAWGAVGAALAAGWWARPGRRVERTDLPLAASG
                     LTEPTPADHLEASGEPGPRGPAA"
     gene            2082..3812
                     /gene="polX"
                     /locus_tag="VFO47_08590"
     CDS             2082..3812
                     /gene="polX"
                     /locus_tag="VFO47_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET8788793.1"
                     /translation="MPWGNEELARLFSEMAELLVLTGETGFRVRAYDRGARALSGYGA
                     DLSGLSERELAAIPGVGKAIAAKVREYLETGRVAKLEELRAEVPPGLRALVRVPGLGP
                     AKARLVHERLGVSSPEELAEAVAAGRVAGLPGMGARTEANLRRALERMARRMDGIPLA
                     VALPLAEALRDRLAGVAGVERVEVAGSLRRMKAAVHDIDLLAAATDPAAVARAFTELP
                     QVAEVAALGDTKASIRTRDGLQVDLRTVRPEVWGAALQYFTGSAAHNVRIRERAVKAG
                     LKLSEYGLFERGGELIVAEDEEVVYDRLGMAWVPPVLREDLGEVDAARRGELPELVTL
                     ADVGGDFHCHTDLSPDGTAPLEVMVDAARARGYRFLAITDHGERLGLGGASRSQLLAQ
                     RQRIRALERRLGDITLLHGAELNIGGDGSLDYDDEFLDGFDVLVASVHDRLDQPRELL
                     TARLVAACEHPAVNVIGHPTGRRIGSRAAGDVDLETLYRAAARTGTALEVNGSPHRLD
                     LGDEQVRLAHRFGVMLAFGADSHGPGHLANMRFAVATAGRGWTTPDRVINTLPLDDLR
                     RFLAKGRPGR"
BASE COUNT          480 a         1576 c         1408 g          476 t
ORIGIN      
        1 cgacgagcgg gccgcggccg ccgtggccgc caagtcccag gtcgacctgg ccgtcgacct
       61 cggcgtcggt ccggccgcgg ccaccgtgac caccaccgac ctcccgcccg aggctctggg
      121 ccgccacggc gggtagacga ggcgctccgg ccgtgcccgc cccggccagc tcggcaaccc
      181 agaaaggctc cacatgccgt ccaatcgcgt gctcgtggtc gaggacgatc cgagcgtgcg
      241 cgggctgctc cagacgatcc tggaggacga agccctggag gtcatcctgg ccgccgacgg
      301 cgaggagggg ctgcggctgg cccgcaccgt cgaccccgac gtcgtcctgc tggacgtgat
      361 gatgcctggg ctgggcgggc ccgacgtcat ccggcgcctg cgccgggccg acggctcgct
      421 gccgttcgcg gtcctggtgg tgaccggggc ggccgaggtg atcgccccgc tgcgcagcga
      481 gctcggcccc gaggccgtcc tcgagaagcc cttcgacatc accaccctgg ccacgcgggt
      541 caagtcgctg gccagcggga acgcctcagg ggagcgtcca tgagcaaacg agacttcctg
      601 tccatcgaca acgtcgagcc cggcgagctg accgccatcc tcgacggggc ggacgggcac
      661 aaggccgacc ggcgccgccg cgacaccctt ggcggccgca gcgtcgccct cgtcttcgag
      721 aagccctcga cccgcacccg ggtctccttc gaggtcgccg tccacgagct cggcggctac
      781 ccgctgcccc tggccggggc cgagctccag ctcggccggg gcgagaccct cgaggacacc
      841 gccgccgtcc tgtcccgcta cgtccacgcg atcgtgctgc gcaccttcgc ccaagagagc
      901 ctggagcggc tggccagggg cggcaccatc ccggtggtca acgccctgtc cgactactcc
      961 cacccctgcc aggccctggc cgacctccag accatccgcg agcgcaaggg ctcgctcgcc
     1021 ggcatccgcc tggcctacct gggcgacggc aacaacgtcg cccacagcct gctgttcgcc
     1081 ggggccaaga tgggcatgca cgtggccgtg gccaccccgg tcggctacga gcccatcccc
     1141 caggtggtcc gccgggcgac cgagatcgcc gccaccaccg ggggcggcgt cgagctgacc
     1201 cacgacccgt ccaccgccgc caaggacgcc gacgtgctct acaccgacgt ctgggcgtcc
     1261 atgggcaagg agatggagca cggcgagcgg gccctgatct tcaagcccta ccagctgtcc
     1321 accgacacgg tgacagccgc cgccccggac gtgatggtgc tgcactgcct gcccgcccac
     1381 cggggcgagg agatcgccgc cgaggtcatg gacggcccca actcggccgt gttcgcccag
     1441 gccgagaacc gcctccacac ccagaaggcg ctgctgtcgt ggctgctcac cggctccacc
     1501 gacccgagct gaccgggtga ccgagccgga cgaccacggc ggccgcccgg ggcggtcccc
     1561 cggtcgatcg gcctggcgct gggccgtggc cgccggcctg gccggggtgg cggcctccat
     1621 cctgaccacc gccctggccg ccgccgtcgc cggccgcgag tccgacctcg ccctgcaacc
     1681 ggtcgagctg gccctgcggg tcgggctgct gctgatcgcc ccctgcgtcg gggccatcct
     1741 ggccgccgtg gtcgtgcccg tccgctcgga gcacggcggc cggctggccg cgctggcggt
     1801 ggggctggcc gtgccgctcg gcccgctgct ggtgctgcgg ctggaccgga tgcgcgagtt
     1861 cggcctcgcc tcggcggcgc tcaccgtcgg cgtcatggcc gcctggggag cggtcggggc
     1921 cgccctggcc gccggctggt gggcccgtcc cggccgccgg gtcgagcgca ccgacctgcc
     1981 cctggccgcc tccggcctga ccgagccgac gccggcggac cacctcgagg ccagcggcga
     2041 gcccgggccc aggggcccgg ccgcctgagg gagaggacgc cgtgccctgg gggaacgagg
     2101 agctggcccg gctgttctcc gagatggccg agctgctggt cctcaccggc gagaccggct
     2161 tccgggtccg ggcctatgac cgcggcgccc gcgccctgtc cggctacggc gccgacctgt
     2221 cggggctgtc cgagcgcgag ctggccgcca tccccggggt cggcaaggcg atcgccgcca
     2281 aggtccgcga gtacctggag acgggccggg tggccaagct ggaggagctg cgggccgagg
     2341 tcccgcccgg ccttcgcgcc ctggtccgcg tccccgggct cggcccggcc aaggcccgcc
     2401 tggtccacga gcggctcggg gtgtcctcgc ccgaggagct ggccgaggcg gtcgccgccg
     2461 ggcgcgtggc cggcctgccc gggatgggcg cgcgcaccga ggccaacctg cgccgcgccc
     2521 tggagcggat ggcccggcgc atggacggca tccccctggc cgtggccctg cccctcgccg
     2581 aggccctccg cgaccggctg gccggggtcg ccggggtcga gcgggtcgag gtcgccgggt
     2641 cgctgcggcg catgaaggcc gccgtccacg acatcgacct gctcgccgcc gccaccgacc
     2701 cggccgccgt ggcccgcgcc ttcaccgagc tgccccaggt ggccgaggtc gccgccctcg
     2761 gcgacaccaa ggcgtcgatc cggacccggg acggcctcca ggtcgacctc cggacggtcc
     2821 ggcccgaggt gtggggggcc gccctccagt acttcaccgg gtcggccgcc cacaacgtcc
     2881 gcatccggga acgggccgtc aaggccgggc tcaagctcag cgagtacggg ctgttcgagc
     2941 ggggcggcga gctgatcgtg gccgaggacg aggaggtcgt ctacgaccgc ctcggcatgg
     3001 cctgggtgcc gccggtgctg cgcgaggacc tgggcgaggt cgacgccgcc cgccgcggcg
     3061 agctgccgga gctggtcacc ctggccgacg tcggcggcga cttccactgc cacaccgacc
     3121 tgtcgcccga cgggaccgct cctcttgagg tgatggtcga cgcggcccgg gcccgcggct
     3181 accggttcct ggccatcacc gaccacggcg agaggctcgg cctgggcggg gccagccgct
     3241 cccaactgct ggcccagcgc cagcggatcc gcgccctgga gcggcgcctg ggcgacatca
     3301 cgctcctcca cggggccgag ctgaacatcg gcggcgacgg gtcgctcgac tacgacgacg
     3361 agttcctgga cggcttcgac gtgctcgtgg ccagcgtcca cgaccgcctc gaccagcccc
     3421 gcgagctgct cacggcccgc ctggtggccg cctgcgagca ccccgcggtc aacgtcatcg
     3481 gccaccccac cggccgccgt atcggcagcc gggccgccgg cgacgtcgac ctggagacgc
     3541 tgtaccgggc ggccgcccgc accggcaccg ccctggaggt caacggctcc ccccaccgcc
     3601 tcgacctggg cgacgagcag gtccgcctgg cccaccggtt cggggtcatg ctcgccttcg
     3661 gcgccgacag ccacggcccc ggccacctgg ccaacatgcg cttcgccgtc gccaccgccg
     3721 gccgcggctg gaccaccccc gaccgggtga tcaacacgct ccccctggac gacctgcgcc
     3781 ggttcctcgc caagggccgc ccgggtcgat gagcccgggt cccaatccgc ccgcgccgga
     3841 accccaccca tctggtgctg gggcccccga tggtgctggg gcccccgaac gggagggtag
     3901 cccgacgcca gagcggacgg cgccggctgt ccacaccccc
//
LOCUS       DASSWR010000512         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_102014,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000512 DASSWR010000000
VERSION     DASSWR010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102014"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1582
                     /locus_tag="VFO47_08595"
                     /pseudo
     CDS             210..1582
                     /locus_tag="VFO47_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
     gene            1679..>2358
                     /locus_tag="VFO47_08600"
     CDS             1679..>2358
                     /locus_tag="VFO47_08600"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874161.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET8788794.1"
                     /translation="MCERPYELGRPEEGYPVRIRARGNQVLTTPMLNRGTAFTPEERE
                     ALGLTGLLPGGVSTIDGQLRRVYAQYLRQPDDLAKNVYLSHLRDRNEVLYYRLLTERI
                     QEMLPIVYTPTIGKAIERYSHEYRRPRGVYLSVDQPQDVESAFRNYGLSADDVDLIVA
                     TDAEGILGIGDQGVGGIDIAIGKLAVYIAAAGIHPRRVIPVVLDMGTDNLALLNDEMY
                     LGNRHARVR"
BASE COUNT          358 a          862 c          762 g          376 t
ORIGIN      
        1 ggccaggctc cgtctggcca gccaggaccg gaccggctgg ttcccgcgga tcggcttcat
       61 ggccatcacc cgtgaacgtt atctacgcaa tcgtgaccga ccaggcggcc ggccgccaga
      121 gtcatctcac gtcatcggca ccgcccacga acccggcggc gcgcaccggg tgagcacagg
      181 cggcgccaac ctcacggatt ggaggccaca tgtccgcaac atcggccgcc acacggcggg
      241 accgcagcca ctacctctac gtcgccgtga ttgccgcggt tgcggccggc atcgtcgtcg
      301 gcttcgccgc cccggcgtcg ccaccgagct caagccgctg ggcactggtt tcgtgaacct
      361 gatcaagatg atgatcagcc cggtcatctt ctgcacgatc gtgctgggca tcggctcggt
      421 ccgcgacgcc accaaggtcg gcaaggtggg cggtctggcg ctcgcctact tcctcgccat
      481 gtccaccgtg gccctggtga tcggcctcgt tgtcggcaac ctgctccacc ccggtgccgg
      541 cctgcacctg gccgggtcgg gggccgaggt gcccgccgcc gagcacggca gcacggtcga
      601 cttcctgctc ggcatcatcc ccaccacgct ggtctccccc ctcactgggg agtccgtgct
      661 ccagaccctg ttcgtggccc tgctcgtcgg cttcgccgtg caggccctcg gcgactccgg
      721 caaggcggtg ctgcgcgggg tcaagcactt cgagcgcgtg gtcttccgcg tcctgtcgat
      781 gatcatgtgg ctggccccga tcggtgcgtt cggggcgatc gccgcggtcg tcgggagcac
      841 cggcctcgat gccctcaagg gcctgggcca gatcatgctc gccttctacc tcacctgctt
      901 cgtgttcgtc atcgtggtcc tcggcctgat cctgcggctc acgacgggga tcggcatcgt
      961 ctcgctgctg cgctacctgg cccgcgaata cctgctaatc gtctccacct cgtcctcgga
     1021 gacggccctt ccccgtctgg tggcgaagat ggagcacctg ggtgtctcgc ggccggtggt
     1081 tggcatcacg gtgccgaccg ggtactcgtt caacctcgac ggcacggcga tctacctgac
     1141 gatggcgtcg ctgttcatcg ccagcgcgct gggcaagccg ctgagcctgg gcgagcaggt
     1201 ctctctcctg ctgttcatgg tcatcgcctc caagggcgcc gccggcgtga ccggtgcggg
     1261 cctggccacc ctggccggtg gcctgcagtc gcaccggccc gacctggtcg acggcgtcgg
     1321 cctcatcgtc gggatcgacc ggttcatgtc cgaagccaga gcggtcacca acttctccgg
     1381 caacgccgtg gccaccgtcg tcatcggcaa gctcgtcggg gagctcgacg gcgagcaggc
     1441 gaaggccgtc ctgagcggca aggagccatt cgacgaagag accatgcttg acgacgtcga
     1501 accgccaccg ctggccgagc acccagtggg gcgggtacac ccgaggcagc cggccgtccc
     1561 ggcccagccc accccagcct gaccgtgcgg ggtcgatccg gccggcatgc ccgcgcagcc
     1621 ggccggatcc acccgctcag gcgagaagct acccgacgag gagccaggag gagctgagat
     1681 gtgcgagaga ccgtacgagc tgggacgccc cgaggagggg tacccggtcc ggatccgcgc
     1741 tcgtggcaac caggtgctga cgaccccgat gctcaaccgg gggaccgcgt tcacgcccga
     1801 ggagcgcgag gcgcttgggc tgaccgggct cctgcccggc ggcgtgtcca cgatcgacgg
     1861 ccagctgcgg cgagtctatg cgcaatacct gcgccagccc gacgacctgg ccaagaacgt
     1921 ctacctcagc cacctacgcg accgcaacga ggtgctctac taccggctgc tcaccgagcg
     1981 catccaggag atgctcccaa tcgtctacac cccgaccatc ggcaaggcga tcgaacgcta
     2041 cagccacgaa taccggcggc cccgcggcgt ctacctgtcg gtcgaccagc cacaggacgt
     2101 cgagtccgcc tttcggaact acggcctcag cgccgacgac gtggacctca tcgtggccac
     2161 cgacgccgag ggcatcctcg gcatcgggga ccaaggcgtc ggcggcatcg acatcgccat
     2221 cggcaagctg gccgtgtaca tcgccgccgc cgggatccat ccccgccggg tgatcccggt
     2281 ggtgcttgac atgggcaccg acaacctggc cttgctcaac gacgagatgt acctgggcaa
     2341 ccggcacgcc cgggtccg
//
LOCUS       DASSWR010000513         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_245583,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000513 DASSWR010000000
VERSION     DASSWR010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245583"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /gene="iolD"
                     /locus_tag="VFO47_08605"
     CDS             <1..1821
                     /gene="iolD"
                     /locus_tag="VFO47_08605"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET8788795.1"
                     /translation="EFLANQHSERDGQAQRLFAGCFGIFGHGNVAGVGQALRQYADRL
                     PYHMARNEQSMVHIASGYAKHRNRLTTFACTSSIGPGATNMVTGAATATVNRLPVLLL
                     PGDIFASRVAPPVLQQQESAAGPDSSANDAFRPVSRYFDRINRPDQLPGSLLEAMRVL
                     TDPAETGAVTLALPQDVQTEAFDYPEELFERRVWHVARPVPEPAMLARAVEVLRGARK
                     PLLVAGGGVIYSEATAALLALVEATGVPVAETYAGKGSMPYDHPSALGAIGVTGTDGA
                     NAMAREADVVVGVGTRWADFATASHSIFTNPEVRFVNLNVAPVDAFKHAAVPLVADAR
                     EGLEALAEALSGWAVDTGWREAAAAHNRDWDDKVERVYRAGHGPLPSQGEVIGAVNDA
                     AEPRDVVVCAAGSMPADLHKLWRTRDPKQFHLEYGNSCMGYEIPGAMGIRLADPSREV
                     FAMVGDAGYLMNPGELVTAVAEGIKIIVVVVQNHGYASIGSLSEASGNGRFGTRFRYR
                     QADGGDGDLLPVDLAANAASLGARVLSPRTVGELADALKEAREADGPVVVHVETDPLE
                     YVTVGGAWWDVAVAEVSTSEDVRRARADYEASKRDQRHYL"
     gene            1848..>2071
                     /locus_tag="VFO47_08610"
     CDS             1848..>2071
                     /locus_tag="VFO47_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8788796.1"
                     /translation="MRTINHWINGQIVATSAERTGAVYDPATGQQTAQVAYATGDDVD
                     AAVRAAQAAFPAWRDTSVVKRARVMFRFLEL"
BASE COUNT          296 a          807 c          713 g          255 t
ORIGIN      
        1 gagttcctgg ccaaccagca cagcgagcgc gacggccagg cccagcgcct gttcgccggc
       61 tgcttcggga tcttcggcca cggcaacgtc gccggcgtcg gccaggccct gcgccagtac
      121 gccgaccggc tcccctacca catggcccgc aacgagcaga gcatggtcca catcgcctcc
      181 ggctacgcca agcaccgcaa ccggctgacc accttcgcct gcacctcctc gatcgggccg
      241 ggcgcgacca acatggtcac cggggcggcc accgccaccg tcaaccgcct gccggtgctg
      301 ctcctgcccg gcgacatctt cgcctccagg gtggccccgc ccgtgctcca gcagcaggag
      361 tcggcggccg gcccggacag cagcgccaac gacgccttcc ggccggtgtc gcgctacttc
      421 gaccgcatca accggcccga ccagctcccc gggtcgctgc tggaggccat gcgggtactg
      481 accgacccgg ccgagaccgg cgccgtcacc ctggccctgc cccaggacgt ccagaccgag
      541 gccttcgact accccgagga gctgttcgag cgccgggtct ggcacgtggc ccggcccgtg
      601 cccgagccgg cgatgctggc cagggcggtc gaggtgctcc ggggagcccg caagccgctg
      661 ctcgtggccg gcggcggggt catctactcc gaggccacgg ccgccctgct ggccctggtc
      721 gaggcgaccg gcgtcccggt ggccgagacc tacgccggca agggctcgat gccctacgac
      781 cacccgtcgg cgctcggggc catcggtgtc accgggaccg acggggccaa cgccatggcc
      841 cgcgaggccg acgtggtcgt cggggtcggc acgcgctggg ccgacttcgc caccgcctcc
      901 cacagcatct tcaccaaccc cgaggtccgc ttcgtcaacc tcaacgtcgc tcccgtggac
      961 gcgttcaagc acgccgccgt cccgctggtg gccgacgccc gggagggcct ggaggccctg
     1021 gccgaggcgc tttccggctg ggccgtggac accggctggc gggaggccgc ggccgcccac
     1081 aaccgggact gggacgacaa ggtcgagcgc gtctaccggg ccgggcacgg ccccctgccc
     1141 agccagggcg aggtcatcgg ggccgtcaac gacgccgccg agccccgcga cgtggtcgtc
     1201 tgcgccgccg gcagcatgcc cgccgacctc cacaagctgt ggcggacccg cgaccccaag
     1261 cagttccacc tggagtacgg gaactcctgc atgggctacg agatccccgg ggccatgggc
     1321 atccggctgg ccgacccgag ccgcgaggtg ttcgccatgg tcggcgacgc cggctacctg
     1381 atgaacccgg gcgagctggt cacggccgtg gccgagggca tcaagatcat cgtcgtcgtc
     1441 gtccagaacc acggctacgc ctccatcggg tcgctgtcgg aggcgtcggg caacggccgc
     1501 ttcgggacca ggttccgcta ccgccaggcc gacggcgggg acggcgacct cctccccgtt
     1561 gacctggccg ccaacgcggc cagcctcggg gccagggtgc tcagcccgcg cacggtcggg
     1621 gagctggccg acgccctcaa ggaggcgcgg gaggcggacg ggccggtggt cgtccacgtc
     1681 gagaccgacc cgctcgagta cgtgaccgtc ggcggcgcct ggtgggacgt ggccgtggcc
     1741 gaggtctcca cctccgagga cgtgcgtcgg gcccgggccg actacgaggc cagcaaacga
     1801 gaccagcggc actacctgta gcgcccaacc tggcgggagg acagcccatg cggaccatca
     1861 accactggat caacggccag atcgtcgcca cctcggccga gcgcaccggg gccgtctacg
     1921 acccggccac cggacagcag accgcccagg tggcctacgc caccggcgac gacgtcgacg
     1981 ccgccgtccg ggccgcccag gccgccttcc cggcctggcg tgacacctcg gtcgtcaagc
     2041 gggcccgggt catgttccgg ttcctggagc t
//
LOCUS       DASSWR010000514         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1120746, whole genome shotgun sequence.
ACCESSION   DASSWR010000514 DASSWR010000000
VERSION     DASSWR010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120746"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFO47_08615"
     CDS             complement(<1..732)
                     /locus_tag="VFO47_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8788797.1"
                     /translation="MNDPVALVDDLVGDPDYRGQLVHLERLPARPARLAAPPDDLPEV
                     VRSRLAARGVEALWSHQAAAVALARAGRNVVVATGTASGKSLCYQLPVFDALLGDGHR
                     TALYLSPTKALARDQLRAVRSFALAGIRASAYDGDTPSADRPMIRRSANLVLTNPDML
                     HRGILPGHERWAAFLGRLAFVVVDEAHTLRGVFGSHVAAILRRLRRLAARYGASPTFV
                     LASATLGNPAELATRLTGLPVEAVTE"
     gene            complement(779..1714)
                     /locus_tag="VFO47_08620"
     CDS             complement(779..1714)
                     /locus_tag="VFO47_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET8788798.1"
                     /translation="MTVTRRRGLTWVTVLGLVAALAAGLVVATSPRKAATAQDGPAIT
                     VQRAHEGSATPALKRRIVLLTIGSDAGAPKFGRGGSVEGGRADALQLVVVDTAKRRGV
                     VLSFPRDSYVNIPGHGTNKINAAMAFGGPKLLVATFEQLTGLTIDYYALTSFDGLTDI
                     VNEVGGVQVNVDMNLRDAFAGAFLNKGKQKLNGRQALAYARARKTLPGGDFDRSRHQG
                     QLLVGGLGTFQRQIAEDPAKMMTWLGVVRDEVKTDLPFPELLRLALLANKVPPGGIRN
                     VPVPSAAGSAGGASVVRLQPGAYALFGRLRAGKLG"
     gene            complement(1711..2298)
                     /locus_tag="VFO47_08625"
     CDS             complement(1711..2298)
                     /locus_tag="VFO47_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788799.1"
                     /translation="MIGPAGVLAAVLAVAGCTSGDRSEQAGQAPGSTANPAAPARPLQ
                     VKLVATARVPPGGPSAKRAAKQAAPGLERFLDRYLTAAYVDAAGGEAGWDELLGLFDK
                     PVRASARKQLDALSLGAAAAKVSAVRPGRASARAVVLYGDRRPAAATVRLAFDGTADT
                     AQGSGQVHLRSVLQLLATGEGWRIADFDSRTGPLR"
     gene            2450..2794
                     /locus_tag="VFO47_08630"
     CDS             2450..2794
                     /locus_tag="VFO47_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8788800.1"
                     /translation="MDLDLNVRKDGDHSVLEVAGEIDVYTAPKLREQLIELVSDGSYH
                     IVVDLEKVDFLDSTGLGVLVGGLKRVRNHDGSLQLVCTQEKILKIFRITGLTKVFPIH
                     SSVGDAISAPAE"
     gene            2804..3271
                     /locus_tag="VFO47_08635"
     CDS             2804..3271
                     /locus_tag="VFO47_08635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788801.1"
                     /translation="MDVDAGGLPATVRLAFPPEPRLLGTVRLVVGIVARKAGLGEEGI
                     EDLKVAVSETCAVAVGDLNKAGRPDPIEVDLVEGADRFGIEVRDRAPAHPAGAGHSAD
                     GEVDDRELGLALVGALVDDLKTSTLEDGGTRTTFWLRRGPGGLGPDDLPPPLT"
     gene            3492..>4624
                     /locus_tag="VFO47_08640"
     CDS             3492..>4624
                     /locus_tag="VFO47_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET8788802.1"
                     /translation="MSPVRLLAQEASAEVEFGSGENLALFAIMAVCVVALLVAFLLVR
                     QVLAADQGTDKMREIARAIQEGSRAYLNRQFRTLAVFVVLLFLLLLLLEADSGSVRIG
                     RSVAFLVGAVFSALAGYTGMQLAVRANVRVAAAADQGGFLKAMPIAFRAGGVAGMFVV
                     GLGLLGAAGVVLVYRGDAASVLFGFGFGAALLAMFMRVGGGIFTKAADVGADLVGKVE
                     QSIPEDDPRNAATIADNVGDNVGDCAGMAADLFESFEVTLVAAIVLGLAAFPDRPEVG
                     LLFPLVVQAIGIVSSIAGIYLVRPRSETESGMRTINRGFFAAAAISLVLVGIASIVYV
                     EDWRPVAAVALGLALSTVIQLMTSYYTGTQYKPVREIAEASETG"
BASE COUNT          580 a         1652 c         1740 g          652 t
ORIGIN      
        1 ctcggtcacc gcctcgacgg gcaggccggt cagccgggtg gccagctcgg ccgggttgcc
       61 cagggtggcc gaggcgagca cgaaggtggg gctggccccg taccgggcgg ccaggcggcg
      121 cagacgtcgc aggattgcgg ccacatgcga gccgaacact ccgcgcaggg tgtgggcctc
      181 gtcgacgacc acgaaggcga gccggcccag gaaggccgcc cagcgctcgt ggccggggag
      241 gatgccccgg tggagcatgt cggggttggt caggaccagg ttggcgctgc gccggatcat
      301 cggccggtcg gcggacgggg tgtcgccgtc gtaggccgac gccctgatcc cggcgagggc
      361 gaaggaacgg acggcgcgga gctggtcgcg ggccagggcc ttggtggggg agaggtacag
      421 ggcggttcgg tggccgtcgc cgagcagggc gtcgaacacc gggagctggt agcagaggct
      481 cttgccggag gcggtgccgg tggccaccac cacgttgcgc ccagcccggg ccagggcgac
      541 ggccgccgcc tggtgggacc agagcgcctc caccccccgg gcggccagcc gcgagcgcac
      601 cacctcgggc aggtcgtccg gcggtgccgc aagccgcgcc ggccgggccg gcaggcgctc
      661 caggtgcacg agctggcctc ggtagtccgg gtccccgacg aggtcgtcca ccagcgcgac
      721 agggtcattc acgccgtcat ggtagccgcc gcccggacat cgccggtcgc ggccgtcctc
      781 agccgagctt gccggcgcgg agccggccga acagggcgta ggcgccgggc tggaggcgga
      841 ccaccgacgc cccgccggcg ctgccggccg cgctcggcac gggcacgttc ctgatgccgc
      901 cggggggcac cttgttggcc agcagggcca ggcgcagcag ctccgggaac ggcaggtcgg
      961 tcttgacctc gtcgcggacg acgccgagcc aggtcatcat cttggccggg tcctcggcga
     1021 tctggcgctg gaaggttccc aggccgccga ccaggagctg gccctggtgg cgggagcggt
     1081 cgaagtcgcc gccgggcagg gtcttgcggg cgcgggcgta ggcgagcgcc tggcggccgt
     1141 tgagcttctg cttccccttg ttcaggaagg cgccggcgaa ggcgtcgcgc aggttcatgt
     1201 cgacgttgac ctggaccccg ccgacctcgt tgacgatgtc ggtgagcccg tcgaacgagg
     1261 tcagcgcgta gtagtcgatg gtcaggccgg tgagctgctc gaaggtcgcg accagcagct
     1321 tcgggccgcc gaaggccatg gccgcgttga tcttgttggt gccgtggccg gggatgttga
     1381 catagctgtc gcgggggaag ctgagcacga ccccgcgtcg cttggccgtg tcgacgacga
     1441 ccagctggag ggcgtcggcc cggccgccct cgacgctgcc gccgcggccg aacttgggcg
     1501 cgcccgcgtc cgacccgatg gtcagcagga cgatccgccg cttgagggca ggcgtggccg
     1561 agccctcatg ggcccgctgg accgtgatcg ccggcccgtc ctgggcggtg gccgccttcc
     1621 ggggcgaggt ggccacgacc aggccggcgg ccagggccgc caccagcccg agaacggtga
     1681 cccaggtcag gcccctgcgc cgcgtgaccg tcacctgagc ggccctgtgc ggctgtcgaa
     1741 gtcggcgatc cgccagccct cgccggtggc cagcagctgc agcaccgagc ggaggtggac
     1801 ctgcccgctg ccctgggcgg tgtcggcggt gccgtcgaag gcgagccgga cggtggcggc
     1861 cgcgggccgc cggtcgccgt acaggaccac cgcccgggcg ctcgcccggc ccggccggac
     1921 ggcgctcacc ttggccgcgg ccgcgcccag ggacagggcg tcgagctgct tgcgggccga
     1981 cgccctgacc ggcttgtcga acagcccgag cagctcgtcc cagcccgcct cgccgccggc
     2041 cgcgtcgacg taggcggcgg tcaggtagcg gtccaggaac cgctccagcc ccggggcggc
     2101 ctgcttggcc gcgcgcttgg cgctcgggcc gcctgggggg accctggccg tggcgaccag
     2161 cttgacctgg agcggccggg ccggggcggc cgggttggcg gtggagccgg gggcctgccc
     2221 ggcctgctcg gaccggtcgc ccgaggtgca gccggcgacg gccaggacgg ccgcgagcac
     2281 gccggcgggc ccgatcatgc gcagcctggg gttcctgtga acagccacga tcacctgttc
     2341 ggagaggttt gcaaggcgcc gcgtaggcta ccaaaccccc ctgacgttgc cctgagacgc
     2401 ccaggcggcc tatcgttgca cgtcctcagc ccgcgagact ggagagagga tggatctcga
     2461 cctcaacgtg cgcaaggatg gcgaccactc ggtgctcgag gtggccggcg agatcgacgt
     2521 ctacaccgcg ccaaagctgc gagagcagct catcgagctg gtcagcgacg ggtcctacca
     2581 catcgtcgtc gacctggaga aggtcgactt cctggactcg accgggctcg gggtgctggt
     2641 cggtgggctg aagcgggtcc gcaaccacga cggctcgctg cagctggtct gcacccagga
     2701 gaagatcctc aagatcttcc ggatcaccgg actgaccaag gtcttcccca tccacagctc
     2761 ggtcggcgac gccatctcgg ccccggccga atagcgtccg gacgtggatg tcgacgccgg
     2821 cgggctgccg gccaccgtcc gcctggcctt cccgccggag ccgcggctgc tgggcaccgt
     2881 ccgcctggtc gtcggcatcg tcgcccgcaa ggccgggctg ggcgaggagg gcatcgagga
     2941 cctcaaggtg gccgtgtccg agacctgcgc ggtcgcggtc ggcgacctga acaaggccgg
     3001 ccgccccgac ccgatcgagg tcgacctggt cgagggggcc gaccgcttcg ggatcgaggt
     3061 ccgcgaccgg gccccggcgc acccggcggg tgccggccac tccgccgacg gcgaggtcga
     3121 cgaccgggag ctcggcctcg ccctggtcgg cgccctggtc gacgacctca agacctcgac
     3181 cctggaggac ggcggcaccc gcaccacctt ctggctccgg cgcgggccgg gcggcctggg
     3241 ccccgacgac ctgccgccgc ccctcacctg aggcgtcggc gacctttcat gggttgcccg
     3301 gttctccggc ccgtggctat gatccgccca atcctcgcgg gctcgcggga cggccggttc
     3361 cctgggcagg tatccgggac gacgccgggc cggggcccgc gctcgactct ttccgctcga
     3421 accgtccagg gcctcggcct ggacaccggg cttgatccaa cgccccagcg ctggggcgtc
     3481 gaggaggtct gatgagccca gtacggctgc tggcgcagga ggcaagcgcc gaggtcgagt
     3541 tcggctccgg ggagaacctg gccctgttcg ccatcatggc ggtctgcgtc gtcgccctgc
     3601 tggtcgcgtt cctcctggtc cggcaggtcc tggccgccga ccagggcacc gacaagatgc
     3661 gcgagatcgc ccgggccatc caggagggca gccgcgccta cctgaaccgg cagttccgca
     3721 ccctggccgt cttcgtcgtc ctgctgttcc tcctcctgct gctgctggag gccgactccg
     3781 gctcggtccg gatcggccgg tcggtcgcct tcctggtcgg ggccgtcttc tcggccctgg
     3841 ccggctacac cggcatgcaa ctggccgtgc gggccaacgt gcgggtcgcg gccgcggccg
     3901 accagggcgg gttcctcaag gccatgccga tcgccttccg ggccggcggg gtggccggca
     3961 tgttcgtggt cggcctgggc ctgctcggcg ccgccggggt ggtcctcgtc taccggggcg
     4021 acgccgccag cgtgctgttc ggcttcggct tcggggcggc cctgctggcc atgttcatgc
     4081 gggtcggcgg gggcatcttc accaaggccg ccgacgtggg cgccgacctg gtcggcaagg
     4141 tcgagcagtc gatccccgag gacgaccccc gcaacgcggc caccatcgcc gacaacgtcg
     4201 gcgacaacgt cggcgactgc gccggcatgg ccgccgacct gttcgagtcc ttcgaggtga
     4261 cgctggtcgc ggcgatcgtg ctcggcctgg ccgccttccc cgaccgcccc gaggtgggcc
     4321 tgctgttccc cctggtcgtg caggccatcg ggatcgtctc gtcgatcgcc ggcatctacc
     4381 tggtccggcc gcgctcggag accgagagcg gcatgcgcac catcaaccgg gggttcttcg
     4441 ccgcggcggc catctcgctg gtgctggtcg ggatcgcctc gatcgtctac gtggaggact
     4501 ggcggccggt ggccgcggtc gccctcgggc tcgccctgtc caccgtgatc cagctcatga
     4561 cctcctatta caccgggacc cagtacaagc cggtgcggga gatcgccgag gcctcggaga
     4621 ccgg
//
LOCUS       DASSWR010000515         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_753374,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000515 DASSWR010000000
VERSION     DASSWR010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753374"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFO47_08645"
     CDS             <1..268
                     /locus_tag="VFO47_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788803.1"
                     /translation="GAAGTGTGAASRQMAAAPVRAKQSAGPAEVAAAWWAARHKVAVD
                     KVRALQQRRVSAKEVQVLVVAEAGPRMPSDYVTVRKGSSGWKVP"
     gene            265..1365
                     /locus_tag="VFO47_08650"
     CDS             265..1365
                     /locus_tag="VFO47_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788804.1"
                     /translation="MRRTVGTKAGAVLVALLTLAMLSSRASPGFPLADVPKSVGRSIA
                     KALGAKPDKPARSKPAAQAGASAFALSDIPRRYLGFYADAAATCKGLTWQVLAAIGKI
                     ESDHGRSSAPGVRSGVNRFGCCAGPMQFNIRNGRPSTWDTWGTGVVAHVYDPAHAVPA
                     AARKLCGDGLARPERIQQDPCPSVLGSAALHVALKRYNNACWYVHEVVTLAGRYTSTA
                     PVASPSKDPFVLALVRNKRITSTSSRGCDPKTDLASGKLDLRVQSLLAVIAERHHVRL
                     SCLRSGHSRFVKGTTRVSNHTVWRAVDIDVVDGRPVSRASPAARELVLWLDGLQGPLR
                     PSEIGSPFEIGRRPYFSDEGHQGHVHVGYRDG"
     gene            complement(1350..2657)
                     /gene="hemL"
                     /locus_tag="VFO47_08655"
     CDS             complement(1350..2657)
                     /gene="hemL"
                     /locus_tag="VFO47_08655"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET8788805.1"
                     /translation="MTGTERSEALFERASRVMPGGVNSPVRAFGGVGGTPRFFVRGRG
                     PYLEDVDGTRYVDLVCSWGPLIAGHAHPRVVEAVQAATARGTSFGAPTEAEVELAEEV
                     GRRVPAVEKLRMVSSGTEATMSAARLARAATGRPKLVKFAGCYHGHADALLAAAGSGV
                     ATLGLPDSAGVTPAQTSHTIVIPYNDEPALAAAFDTDGDQIAAVMVEPIAANMGVVPP
                     TPGFLAALRSQTRRAGALLILDEVMTGFRVHRGGATGLWSLEPDLLTFGKVLGGGLPA
                     AAYGGPAELLGLVAPEGPMYQAGTLSGNPLATAAGLATLSLLDDHAYRHLDGLAGALC
                     DGLGDAFEAAGVPARVQLAGNLFSVFMTADEVGDYQAAQAQDTAAYARFFHAMLDRGV
                     YLPPSAYEAWFVSIVHTDAEVDRVLAAAREAAKAAAVSAQPSR"
     gene            complement(2654..3538)
                     /locus_tag="VFO47_08660"
     CDS             complement(2654..3538)
                     /locus_tag="VFO47_08660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8788806.1"
                     /translation="MRHRRIGSTDLVASEIGLAVWPLVAGPGARSDEDVAGLLKAALD
                     LGISYFGASDSDGDGRAEELLGQAVRGHRDEVTVATTFGYDTTPRPWEPAPAVPRHDW
                     SAAFAGRALDRSLGRLRLEPVDLWLLHHPGMDAVESDALFEFLEAQVAKGKVRYYGVA
                     LGPGPGWGDEGAAALQERRVAAVETVYNLTEQDPGRELAALAAETGAGLIARDPLTPR
                     LAAADRARLEFLERDRHQTLDQALLRFALADPAVATTLPEVHDHGHLAELAGAADLDP
                     PTPEDLDRIAELREGGAS"
BASE COUNT          450 a         1343 c         1373 g          445 t
ORIGIN      
        1 cggggcggct ggcaccggga ccggcgccgc cagccggcag atggcggcgg cgccggtccg
       61 ggccaagcag tcggcgggcc cggccgaggt cgcggccgcc tggtgggccg cccgccacaa
      121 ggtggccgtg gacaaggtgc gggccctgca gcagcgccgg gtgagcgcca aggaggtcca
      181 ggtgctggtg gtcgccgagg ccggtccgcg gatgcccagc gactacgtga ccgtccgcaa
      241 gggctcgtcg ggctggaagg tgccgtgagg cggaccgtcg gcaccaaggc cggagccgtg
      301 ctggtcgcgc tgctgaccct ggccatgctc agcagccgcg cctcgcccgg attccctctg
      361 gccgacgtgc ccaagtcggt cgggcgctcg atcgccaagg ccctcggcgc caagcccgac
      421 aagccggccc gctccaagcc ggccgcccag gccggcgcca gcgccttcgc gctgtccgac
      481 atcccccgcc gctacctcgg cttctacgcc gacgcggccg cgacctgcaa gggcctgacc
      541 tggcaggtgc tggcggccat cggcaagatc gagtccgacc acggtcgctc ctcggccccc
      601 ggcgtgcgca gcggcgtcaa ccgcttcgga tgctgcgcgg ggccgatgca gttcaacatc
      661 cgcaacggcc ggccgagcac ctgggacacc tggggcacgg gcgtcgtggc ccacgtctac
      721 gaccccgccc acgcggtgcc cgcggccgcc cgcaagctct gcggcgacgg cctggcccgc
      781 cccgagcgga tccagcagga cccctgcccg agcgtgctcg gctcggccgc cctgcacgtc
      841 gccctcaagc gctacaacaa cgcctgctgg tacgtgcacg aggtggtgac cctggccggc
      901 cgctacacct cgacggcgcc ggtggcgtcc ccctccaagg acccgttcgt gctcgccctg
      961 gtccgcaaca aacgcatcac cagcacatcc agccgcggct gcgaccccaa gaccgacctg
     1021 gcctcgggca agctcgacct gcgcgtccag tccctgctgg cggtgatcgc cgagcggcac
     1081 cacgtccggc tgagctgcct gcgctcgggc cactcccggt tcgtcaaggg caccacccgg
     1141 gtctccaacc acaccgtctg gcgggccgtg gacatcgacg tggtcgacgg gcggccggtg
     1201 tcgcgggcca gcccggcggc ccgggagctg gtgctgtggc tcgacgggct ccagggcccg
     1261 ctgcggccgt ccgagatcgg ctcgccgttc gagatcggcc gccgccccta cttctccgac
     1321 gagggccacc aggggcacgt acacgtcggc taccgggacg gctgagcgct gacggcggcc
     1381 gccttggccg cctcccgggc ggcggcaagg acccggtcga cctcggcgtc ggtgtgcacg
     1441 atcgacacga accaggcctc gtaggcgctg ggggggaggt agacgccccg gtccagcatg
     1501 gcgtggaaga agcgcgcgta ggcggccgtg tcctgggcct gggcggcctg gtagtcgccg
     1561 acctcgtcgg cggtcatgaa cacgctgaac aggttgccgg cgagctggac ccgggccggg
     1621 acgccggccg cctcgaaggc gtccccgagc ccgtcgcaga gggctccggc caggccgtcg
     1681 aggtggcggt aggcgtggtc gtcgagcagc gacagggtgg ccaggccggc ggcggtggcc
     1741 agcgggttcc cggacagggt gccggcctgg tacatgggtc cctcgggggc gaccaggccc
     1801 agcagctcgg ccggcccgcc gtaggcggcc gccgggaggc cgccgcccag caccttgccg
     1861 aaggtgagca ggtccggctc cagcgaccac agcccggtgg cgccgccgcg gtggacccgg
     1921 aagccggtca tgacctcgtc caggatgagc agcgccccgg cccggcgggt ctgggaccgc
     1981 agggcggcca ggaatcccgg cgtcggcggg accaccccca tgttggccgc gatgggctcg
     2041 accatcacgg cggcgatctg gtcgccgtcg gtgtcgaagg cggcggccag ggcgggttcg
     2101 tcgttgtagg ggatgacaat ggtgtgactc gtctgggcgg gcgtgacccc cgccgagtcg
     2161 ggcaggccga gggtggccac tcccgagccg gcggcggcca ggagggcgtc ggcgtggccg
     2221 tggtagcagc cggcgaactt gaccagcttg ggccggccgg tggccgcccg ggccagccgg
     2281 gccgcgctca tggtggcctc ggtgccggag ctgaccatgc gcagcttctc gacggccggg
     2341 acccggcggc cgacctcctc ggccagctcg acctcggctt cggtgggggc gccgaacgag
     2401 gtcccgcggg cggtggcggc ctgcaccgcc tcgaccaccc ggggatgggc gtgcccggcg
     2461 atcagcggtc cccacgagca gaccaggtcg acgtagcggg tgccgtccac gtcctccagg
     2521 tacggccccc ggccgcggac gaagaatcgg ggcgtccccc cgaccccgcc gaaggcccga
     2581 acgggcgagt tcaccccgcc gggcatgacc ctggaggccc gttcgaacag ggcctcgctc
     2641 cgctcggttc cggtcaactc gcccctccct cgcgtagctc ggcgatgcgg tcgaggtcct
     2701 cgggggtcgg cgggtccagg tcggccgcgc cggccagctc ggccaggtgg ccgtggtcgt
     2761 ggacctccgg cagggtcgtg gccaccgccg ggtcggccag ggcgaagcgg agcagggcct
     2821 ggtcgagggt ctggtggcgg tcccgctcca ggaactccag ccgcgcccgg tcggccgccg
     2881 ccaggcgcgg ggtcaggggg tcacgggcga tcaggccggc gcccgtctcg gcggccaggg
     2941 cggccagctc ccggcccggg tcctgctcgg tcaggttgta gacggtctcg acggcggcga
     3001 cccgccgctc ctggagggcg gccgcgccct cgtcgcccca gccgggcccg ggaccgaggg
     3061 cgacgccgta gtacctgacc ttgcccttgg ccacctgtgc ctccaggaac tcgaacagcg
     3121 cgtcggactc gacggcgtcc atgcccgggt ggtggaggag ccacaggtcg accggctcca
     3181 ggcgcagccg ccccagcgag cggtcaaggg cgcgcccggc gaaggcggcc gaccagtcgt
     3241 ggcggggcac ggccggggcc ggctcccagg gtcgtggggt ggtgtcgtag ccgaaggtgg
     3301 tggccaccgt gacctcgtcc cggtggccgc gcacggcctg gccgagcagc tcctcggcgc
     3361 ggccgtcgcc gtcgctgtcg ctggccccga agtaggagat cccgaggtcg agggccgcct
     3421 tcagcaggcc ggcgacgtcc tcgtcggacc gcgcccccgg cccggcgacc agcggccaca
     3481 cggccagccc gatctcgctg gccacgaggt cggtcgagcc gatgcggcgg tgacgcactc
     3541 cggtaggctc cttggcgcgt ggcgaccttg gtttggctgg gggacccacc gcccagtcta
     3601 cgagccagac a
//
LOCUS       DASSWR010000516         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_697673,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000516 DASSWR010000000
VERSION     DASSWR010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697673"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFO47_08665"
     CDS             <1..320
                     /locus_tag="VFO47_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET8788807.1"
                     /translation="AAGLGATVVAAGFYQGGAGNVRLGEEFHHNRLTLLASIGAWGAP
                     HRKAPLWNRRRVMETATRLLYTDRVSVDGLLARRFPFAQAPDAYRWIDEHPLDAVKVA
                     LTY"
     gene            427..1389
                     /locus_tag="VFO47_08670"
     CDS             427..1389
                     /locus_tag="VFO47_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8788808.1"
                     /translation="MEAVVAPTDRIKAWAARRPERVLGYNPWQLAAHVGHTALRHRVT
                     GPAAEMSFFAMLTLVPLTVAVGAALGQIQRFVGPEKIARGQDAAISVVRVVISPELTD
                     GIVDPFVRAQLDQRKGGAAIGGLLLTWWLAGRLFAATSRALDAAYHDRHQRLSLQQRV
                     IALGFAFGSVVVVVLTLGLMVDAPFLDEHRDVARQFGVSQALVTAWFIGRWPVALAVL
                     VAFLLCLYRFAPTVRHAWRHLLPGAVLGVVLWILAAGVFRLYLAFGSGAPTGVVVNDA
                     KVVIIGQAVGAVVATVLWTYFSSVAILAGNELNAELARLRGLDA"
     gene            1428..1829
                     /locus_tag="VFO47_08675"
     CDS             1428..1829
                     /locus_tag="VFO47_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HET8788809.1"
                     /translation="MRTPSPRRPLPLLAALLLAGCAGPAGDPATPEPPVPATATAPTA
                     APVAPATQPDADQRPAVRVIASGRVEAGGGPGCLLLATDQGRPWLLVGGDRAVLAAGA
                     RVRVVGEPAPARTAGCQQGEPLRVRDATPAG"
     gene            2004..4856
                     /locus_tag="VFO47_08680"
     CDS             2004..4856
                     /locus_tag="VFO47_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HET8788810.1"
                     /translation="MLRGTPYSLKRPVVALLALALLVAMAAVSQSPPASAGDPASGRA
                     RLAPSPGSWSNPVAPRMDRIGNQQERLGRLSTSASRSRLTPSAKVVAQSEAFDRKHST
                     GNPAAAEVLGRLEARSAKSGKSPRAFKKAPTTQTARLLTVLVEFNPDAGDDFSGFERP
                     AFVGAEECVTEPAGTLLGGPLHNNMPDPATVGRGTDNNTFWVPDFSPSHYNKLIYTKT
                     GLTRRVRPDLTGPDGKPGVDLRGYTVKNHYQEMSKGAYDITGQVAGWVQVPHSEAWYG
                     ATVCGQPMQNNSGHPDNPRQVSQLIVDAVDALAASDPDFPWAHYDIEDQGDADGDGNL
                     FEADGVVDHFVIIHAGADKADGGGAEGPYSIWSHAGNVDPSTGGYAVPGTEVRVNNYI
                     MQAEDAGVGVISHEYGHDLGLPDLYDTSGAADSDVDFWDLMASGSHTGPLFQTIPTHM
                     GLWDKYVLGWVDPETFGVGSSSRDVKVGQTSRTPRGTEDGVRVNLPEKSVTLGTPHSG
                     DNMWYSNNDQSWADVKLSRTLDVPAGADVRFWTWDNYTIEELWDYGFIEVSTDGGATW
                     TQLEVRDEAGNVVSTNEDPNGRLRDYGGLQNGLTGTTGGEWRHDWVNLTPYAGTTIGL
                     RLRYATDAAFEEPGWFADDFEVTADGTSVWTDDVESGADGWTSEQGTFTDTEGAGWII
                     TSGTFIYQHYYLAEWRNYDGFDKGLRYAYDTTYSNGEFGDEWRVTRTPYNAPGMLVWY
                     RDGQYTVNHVTTPIFAPPSTGSKGQLLIVDSHFDPLRRFGEAAEHDTTTLKNLPSRAQ
                     SSNAAFAKRPTRQFRECIEDPVGSYTTYCNVHAPQAGVPHFTDAKGWYPGLELRGEDL
                     FFRDADASVVVPSKDHRRYTTRIVDAEGNPLTDLYGTDLGDGIILGTGNPADGNPLDT
                     PVEDLSLGVEFTVRRVAGSNRWVVIRVNAADAENAP"
     gene            complement(4922..>5815)
                     /locus_tag="VFO47_08685"
     CDS             complement(4922..>5815)
                     /locus_tag="VFO47_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8788811.1"
                     /translation="GFLVTLAVPLCAGATVVTMPRFDPGQFLELLERHRVTVLVGAPP
                     MFQVLLRHPRVAAADLGALELLVAGGAPLAAATQRALATRLPGAIVGQAWGMTETTVG
                     LSMPDRDFGSVPGTVGRVVPSTELRVVDPDSGRDLGPGEPGELLARGPQVMAGYLGRP
                     EATAAMVDPDGWLRTGDLGLVDGDGNVVIVDRLKELIKVSGHQVAPAELEALLATHPA
                     VADAAVLRRDDPARGEVPVAVVVPRPGTDPDPDELLAWVAGRVAPYKRLHAVRFADAI
                     PRTPSGKLLRRALHDQDRQTV"
BASE COUNT          813 a         2233 c         2026 g          743 t
ORIGIN      
        1 aggcggccgg gctgggggcg acggtggtgg cggccgggtt ctaccagggc ggcgccggca
       61 acgtgcgcct tggtgaggag ttccaccaca accggctgac gctgctcgcc tccatcggcg
      121 cctggggcgc gccgcaccgc aaggcccccc tgtggaaccg gcgccgggtg atggagaccg
      181 ccacccggct gctctacacc gaccgggtct cggtggacgg cctgctcgcc cgccggttcc
      241 cgttcgcgca ggccccggac gcctaccgtt ggatcgacga gcatcctctg gacgcggtca
      301 aggtcgccct cacctattag cccgaccggc tcaacccggg cggagcccat ggatcgtcgg
      361 ccgaaggccg actcctgggc gggtctgcca caatatcggg gcagatggtt gatcgatcat
      421 gtgggggtgg aggcggtggt tgccccgacg gatcgcatca aggcgtgggc ggcgcgccgc
      481 cccgagcggg tcctgggcta caacccctgg cagctcgcgg cccacgtggg ccacaccgcc
      541 ctgcgccacc gggtcaccgg cccggcggcc gagatgtcct tcttcgccat gctgaccctg
      601 gtcccgctga ccgtggccgt cggcgccgcc ctcggccaga tccagcggtt cgtcggcccg
      661 gagaagatcg ccaggggcca ggacgccgcc atctccgtgg tccgcgtcgt catcagcccc
      721 gagctgaccg acggcatcgt cgaccccttc gtccgggccc agctcgacca gcgcaagggg
      781 ggcgcggcca tcggcggcct cctgctcacc tggtggctgg ccggccgcct gttcgccgcc
      841 accagccgcg ccctcgacgc cgcctaccac gaccggcacc agcggctgtc gctccagcag
      901 cgggtcatcg ccctcgggtt cgccttcggg tcggtcgtgg tcgtggtcct gaccctgggc
      961 ctcatggtcg acgcgccgtt cctggacgag caccgcgacg tggcccggca gttcggggtc
     1021 agccaggccc tggtcaccgc ctggttcatc ggccgctggc cggtggccct ggccgtcctg
     1081 gtcgcgttcc tgctgtgcct gtaccggttc gcccccaccg tccggcacgc ctggcgtcac
     1141 ctgctcccgg gcgccgtcct cggcgtggtc ctgtggatcc tggccgccgg ggtcttccgg
     1201 ctgtatctcg ccttcggcag cggcgccccg accggggtgg tcgtcaacga cgccaaggtg
     1261 gtgatcatcg gccaggccgt cggggcggtg gtcgcgaccg tcctgtggac ctacttctcc
     1321 agcgtggcca tcctggccgg caacgagctg aacgccgagc tggcccggct gcgcggcctc
     1381 gacgcctagg cggggaaccg ttgccgcccg gcgggcgtcc aaccggcatg cgaaccccgt
     1441 cgccccgccg accgctgccg ctcctggccg cgctcctgct cgccgggtgc gccgggccgg
     1501 ccggcgaccc cgccaccccg gagccccccg ttcccgcgac cgcgaccgcg cccacggccg
     1561 cccccgtcgc cccggccacc cagcccgacg ccgaccagcg ccccgcggtg cgggtgatcg
     1621 ccagcggccg ggtcgaggcc gggggcgggc cgggctgcct gctcctggcc accgaccagg
     1681 gccgtccctg gctgctggtc gggggcgacc gggcggtgct ggccgccggg gcccgcgtcc
     1741 gggtcgtggg ggagcccgcg cccgcccgca ccgccggctg ccagcagggc gagcccctgc
     1801 gggtgcggga cgccaccccc gccggctgac ccccgacgcc gctgtactag gccgagtggc
     1861 gtaggttcag ttgcgcaagc tgcagtaggg cccgatctgt ggcacatgcc aacatttgcc
     1921 gcaattcgcg gggtcattgg cgggtgggaa ccgcaactcc ttcatgtcgg tcaagggccg
     1981 ttcctcagag gggagaaacg accgtgcttc gtggcactcc gtactcgctc aagcggccgg
     2041 tggtcgcgct gctggcattg gcgctgctgg tcgccatggc ggccgtcagc cagtcgcctc
     2101 cggccagcgc cggcgacccg gcgtcggggc gggcccgcct ggcgccgtcg ccgggcagct
     2161 ggtccaaccc ggtggcgccg cggatggacc gcatcgggaa ccagcaggaa cggctcggca
     2221 ggctgagcac gtcggccagc cgctccaggc tcacccccag cgccaaggtc gtcgcccagt
     2281 ccgaggcctt cgaccgcaag cactcgaccg gcaacccggc ggcggccgag gtgctcggcc
     2341 ggctcgaggc caggtcggcc aagagcggca agagcccgcg ggccttcaag aaggccccca
     2401 ccacccagac ggcccggctg ctcacggtcc tggtcgagtt caaccccgac gccggcgacg
     2461 acttctccgg cttcgagcgc ccggccttcg tgggcgccga ggagtgcgtc accgagccgg
     2521 ccggcaccct gctgggcgga ccgctccaca acaacatgcc cgacccggcg acggtcggcc
     2581 gtggcaccga caacaacacc ttctgggtgc cggacttcag ccccagccac tacaacaaac
     2641 tgatctacac caagaccggc ctcacccggc gggtccggcc cgacctgacc ggccccgacg
     2701 gcaagcccgg cgtcgacctg cgcggctaca ccgtcaagaa ccactaccag gagatgtcca
     2761 agggcgccta cgacatcacc ggccaggtcg ccggctgggt ccaggtgccg cactccgagg
     2821 cctggtacgg cgccaccgtc tgcggccagc cgatgcagaa caacagcggc cacccggaca
     2881 acccccggca ggtcagccag ctgatcgtgg acgcggtcga cgccctggcg gccagcgacc
     2941 ccgacttccc ctgggcccac tacgacatcg aggaccaggg cgacgccgac ggcgacggca
     3001 acctgttcga ggccgacggg gtcgtcgacc acttcgtgat catccacgcc ggcgccgaca
     3061 aggccgacgg cggcggcgcc gagggcccct actcgatctg gtcgcacgcc ggcaacgtcg
     3121 acccgtcgac cggcggctac gccgtcccag gcaccgaggt gcgcgtcaac aactacatca
     3181 tgcaggccga ggacgccggc gtcggggtca tctcccacga gtacggccac gacctggggc
     3241 tgcccgacct gtacgacacc agcggcgcgg ccgactccga cgtcgacttc tgggacctga
     3301 tggcgtccgg gtcgcacacg ggcccgctgt tccagaccat cccgacccac atgggcctgt
     3361 gggacaagta cgtgctcggc tgggtcgacc ccgagacctt cggcgtcggc tcctcgagcc
     3421 gcgacgtcaa ggtcggccag acctcgcgga cccccagggg gaccgaggac ggcgtccggg
     3481 tgaacctgcc cgagaagtcg gtcacccttg gcacgccgca cagcggcgac aacatgtggt
     3541 actcgaacaa tgaccagtcg tgggccgacg tgaagctgtc gcgcaccctc gacgtgcccg
     3601 ccggcgccga cgtgcgcttc tggacctggg acaactacac catcgaggag ctgtgggact
     3661 acggcttcat cgaggtgtcg accgacggcg gggccacctg gacccagctc gaggtccgcg
     3721 acgaggccgg caacgtggtc tcgaccaacg aggaccccaa cgggcggctg cgtgactacg
     3781 gcggcctcca gaacggcctc accgggacca ccggcggcga gtggcgccac gactgggtca
     3841 acctgacccc gtacgccggc accaccatcg gcctgcggct gcgctacgcc accgacgccg
     3901 ccttcgagga gccgggctgg ttcgccgacg acttcgaggt caccgccgac ggcacctcgg
     3961 tctggaccga cgacgtcgag agcggcgccg acggctggac ctcggagcag ggcaccttca
     4021 ccgacaccga gggggccggc tggatcatca ccagcggcac cttcatctac cagcactact
     4081 acctggccga gtggcgcaac tacgacggct tcgacaaggg cctgcgctac gcctacgaca
     4141 ccacctactc caacggcgag ttcggggacg agtggcgggt cacccgcacc ccctacaacg
     4201 cccccggcat gctggtctgg taccgcgacg gccagtacac cgtgaaccac gtgaccaccc
     4261 cgatcttcgc gccgcccagc accggctcca aggggcagct gctgatcgtg gacagccact
     4321 tcgacccgct gcggcgcttc ggcgaggcgg ccgagcacga cacgacgacg ctgaagaacc
     4381 tgccgtcgcg ggcccagtcc tcgaacgcgg ccttcgccaa gcggccgacg cgccagttcc
     4441 gcgagtgcat cgaggacccg gtgggctcgt acacgaccta ctgcaacgtc cacgccccgc
     4501 aggccggcgt gccccacttc accgacgcca agggctggta cccggggctg gagctgcgcg
     4561 gcgaggacct gttcttccgc gacgccgacg cctcggtggt ggtcccctcc aaggaccacc
     4621 ggcgctacac cacccggatc gtcgacgccg aggggaaccc gctgaccgac ctgtacggca
     4681 ccgacctggg tgacgggatc atcctcggca ccggcaaccc ggccgacggc aacccgctcg
     4741 acaccccggt cgaggacctc tcgctcggcg tcgagttcac ggtccgccgg gtggccggca
     4801 gcaaccgctg ggtggtgatc cgggtcaacg ccgccgacgc ggagaacgcc ccctagaaca
     4861 gcggtcgagt atccggcgcc gccccccggc gggcctcggt gctccctggg tcctgccccg
     4921 gtcagacggt ctggcggtcc tggtcgtgca gggcccggcg gagcagcttg cccgaggggg
     4981 tccgggggat ggcgtcggcg aagcggacgg cgtggagccg cttgtagggc gccacccgcc
     5041 cggccaccca ggccagcagc tcgtcggggt cggggtcggt cccgggccgg ggcacgacca
     5101 ccgcgaccgg gacctcgccc cgggcggggt cgtcgcggcg caggacggcg gcgtcggcca
     5161 cggccgggtg ggtggcgagc agcgcctcca gctcggccgg ggcgacctgg tggccgctca
     5221 ccttgatcag ctccttgagc cggtcgacga tcaccacgtt gccgtcgccg tcgaccaggc
     5281 cgaggtcgcc ggtgcgcagc cagccgtccg ggtcgaccat ggccgcggtc gcctcgggcc
     5341 ggcccaggta gccggccatc acctggggcc cgcgggccag cagctcaccc ggctccccgg
     5401 ggccgaggtc gcggccgctg tccgggtcga ccacccgcag ctcggtgctg ggcacgaccc
     5461 ggccgaccgt gcccgggacg ctgccgaagt cgcggtcggg catgctcagg ccgacggtcg
     5521 tctcggtcat gccccaggcc tggccgacga tcgcccccgg cagccgggtc gccagcgccc
     5581 gctgggtggc ggcggccagc ggcgccccgc cggcgaccag cagctccagc gcgcccaggt
     5641 cggcggcggc gacccgcggg tgccgcagca ggacctggaa catgggcggc gccccgacca
     5701 ggacggtgac ccggtggcgc tccagcagct ccaggaactg cccggggtcg aagcggggca
     5761 tggtgaccac ggtcgcgccg gcgcacaggg gcacggccag ggtgaccagg aaccc
//
LOCUS       DASSWR010000517         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_951527,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000517 DASSWR010000000
VERSION     DASSWR010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951527"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFO47_08690"
     CDS             <1..328
                     /locus_tag="VFO47_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET8788812.1"
                     /translation="AIDWRRQAARRPTMSVPVEQLAERPAPDDPVAAMFEAQSTRAAL
                     ALLAELPTDQAEVVALRVLGGLEIAEVARIVGKRPGAVRVLAHRGLRRLAKQLEAAGL
                     AGGVTR"
     gene            355..1317
                     /locus_tag="VFO47_08695"
     CDS             355..1317
                     /locus_tag="VFO47_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788813.1"
                     /translation="MHPLLGFLESAPSVADQDMLERLLEGRLDPASAPPGYGGLARLL
                     AAATAPAAPEELAGEQRALAEFMAVMRSTPPTLVPRRIAVPGKVFTMKAAAAALVAVL
                     SVGGIAAAATGHLPGQASSVADRATVTSGADASGQGMGAAASVELDAAAQVGLCRALQ
                     AGQGADHGSRADTAAFQALAAAAGGAGNVVGYCEDITAGASADSQGRESAVGPDATGA
                     ARAGLCRAWQAGQGADHGRRADSVAFQALAAAAGGADKVAAYCEDVTAGAGSDGQGRA
                     SPPSVPATPTPASPPSSGPPADPGPPASTGPGGHGQGGPPTTTG"
     gene            complement(1338..1724)
                     /locus_tag="VFO47_08700"
     CDS             complement(1338..1724)
                     /locus_tag="VFO47_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8788814.1"
                     /translation="MRDAPSVVDLAGLAAAGDRPGALWRLDGEDLQANLIRLGLGDRI
                     QPHRNDEVEVLMVVVAGRGELTLDGQVHPLAPMALVHVPKGAVRAVVAVDGPLAYLSV
                     HRRRSPGLQIGRHAQRAPTGQSWPSG"
     gene            complement(1774..2418)
                     /locus_tag="VFO47_08705"
     CDS             complement(1774..2418)
                     /locus_tag="VFO47_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8788815.1"
                     /translation="MALVACLLLAAVTGCGASPPRRTEPAGSGVPSTLAERPGGRDLV
                     ARLRQGGHVIFIRHAATEATQDDPQPNLADPRTQRNLSDAGRNQARQLGRAIRRLRIP
                     VGTVLASPYARTRETAELAFGPDRVRVTRDLLNEAFPGTDDEDLARRLMRLLAMRPPA
                     GQNAVLVSHGFNLQGATGLSVAEGESAVFAPGGADRSRLVARITAQEWQSLASG"
BASE COUNT          333 a          972 c         1030 g          339 t
ORIGIN      
        1 ggccatcgac tggcgccggc aggccgcgcg tcggccaacc atgtcggtcc cggtcgagca
       61 gttggccgaa cggccagccc ctgatgaccc ggtcgcggcg atgttcgagg cccaatcgac
      121 ccgggcggcg ctggcgctgc tggccgagct gccaaccgac caggccgagg tggtggccct
      181 gcgggtactg ggcggcttgg agatcgccga ggtggccagg atcgtcggca agcgcccggg
      241 ggcggtgcgg gtgctggccc atcgggggtt gcggcggctg gccaaacagt tggaggcggc
      301 cgggttggcg gggggtgtaa cgcgatgagc gacgacgacg ctcttgggcc cgacatgcat
      361 ccactgcttg ggttccttga gagtgcgccg tccgtcgccg accaggacat gctggagcgg
      421 ctgctggaag gccggctcga ccctgcctcg gccccgcccg gctacggtgg gctggccagg
      481 ttgctggcgg cggcgaccgc cccggccgct cccgaggagc tggccggtga acagcgagcc
      541 cttgccgagt tcatggcggt gatgcggtcg acccccccga ccctcgtccc ccggaggatc
      601 gccgtgcccg gcaaggtgtt cacgatgaaa gcggccgcgg ccgcactggt cgccgtcctg
      661 tcagtcggcg ggatcgccgc cgcggccacc gggcacctgc ccggccaggc gtcgtcggtg
      721 gccgaccggg caacggtcac gtcgggtgcc gacgcgtccg ggcagggtat gggggccgcc
      781 gccagcgttg agctggatgc ggcagcccag gtgggcctgt gccgagccct gcaggccggc
      841 cagggcgccg accacggttc ccgggccgac acggcggcgt tccaggccct ggcagcggcg
      901 gccggtggtg ccggcaacgt cgtcggctac tgcgaggaca tcacggccgg tgcgtcggct
      961 gacagccagg gccgggagtc ggcggtcggg ccggacgcca ccggagcggc ccgggccggg
     1021 ctgtgccggg cgtggcaggc cggccagggc gccgaccacg gacgccgggc ggactcggtc
     1081 gccttccagg cgctggcggc ggccgcgggc ggagccgaca aggtcgccgc ctactgcgag
     1141 gacgtgaccg ccggcgccgg cagtgacggc cagggccgcg cctcgccccc cagcgtcccg
     1201 gcgactccga cccccgcctc gccaccgagc agcggcccac ccgcggaccc cggcccaccc
     1261 gccagcaccg gacctggagg gcacgggcag ggcggacccc cgacgaccac cggctgacag
     1321 gtccagaacc cgttcgatca cccggatggc caggactgtc cagtcggggc ccgctgggcg
     1381 tggcggccga tctgaaggcc cggggagcga cggcggtgga ccgacaggta ggcaagcggc
     1441 ccgtcgaccg ccaccacggc cctgaccgcc cccttgggca cgtggacgag ggccatcggg
     1501 gcgagcgggt ggacctggcc gtcgagggtg agctcgcctc ggccggccac gaccaccatc
     1561 aggacttcca cctcgtcgtt ccgatgcggc tggatgcggt cccccaagcc gagccggatc
     1621 aggttggcct gcaggtcctc tccatccagc cgccacaggg cgcctggccg gtccccggcc
     1681 gccgcgaggc cggccaggtc gaccacagaa ggggcgtcgc gcatctcacc atgttctcat
     1741 ccggaccgca ccagacggtc gcagcgcatc ccatcagccc gacgccaggc tctgccattc
     1801 ctgggcggtg atcctcgcga ccagcctgga gcggtccgcg ccgccgggtg cgaagaccgc
     1861 cgactcgccc tccgcgaccg agagtcccgt cgctccctgg aggttgaagc cgtgggagac
     1921 gagcaccgcg ttctgcccgg ccggtggtcg catcgccaac aggcgcatca gccggcgggc
     1981 caggtcctcg tcgtcggtgc cggggaatgc ctcgttcagg aggtctcgtg tcacgcgcac
     2041 gcgatccggc ccgaaggcca gctcggcggt ctcccgggtc cgcgcgtagg ggctggccag
     2101 caccgtgccc accggaatcc ggaggcggcg gatcgcccgg ccgagctgcc gggcctggtt
     2161 gcgtccggca tcggagagat tccgctgcgt gcgcgggtcg gccaggttgg gctgagggtc
     2221 gtcctgggtt gcctcggtcg ccgcgtggcg gatgaagatg acgtgccctc cctggcgtag
     2281 acgggccacg aggtcgcggc cgccgggtcg ctcggcgagc gtggacggga cgccggatcc
     2341 ggctggttcc gtccggcggg gcgggctggc accgcatccg gtgacggcgg cgagcaggag
     2401 gcaggcaacc agcgccacgg cggcgcgtcc cggccgtccc ccgggtctca tgcgggttcg
     2461 cgccaggacg catggacggc gatggtcatg ccaccagtct gcgccggccc gggggctcgc
     2521 gctcgcccgc cgaaccgccg tcgctatcat cgccccatga tgggtgctgc ggcgcggcgc
     2581 acaggacggc tcggctggct gacggtgctc ctgatcctgg tgaccgcatg tgggcggtct
     2641 cccgagccgg gcgccgaccg gccgtcgcgg ccgc
//
LOCUS       DASSWR010000518         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_358528,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000518 DASSWR010000000
VERSION     DASSWR010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358528"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFO47_08710"
     CDS             <1..924
                     /locus_tag="VFO47_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET8788816.1"
                     /translation="VALGQRVEPELREGFLAVYGPLSLLGLLVTWVVLLVMGWGAVWW
                     GLRSAVTGVDGYLDAVYFAGVGFFTVGFGDLVPTGGLARLLVLLEAFMGLVTMALVIG
                     YLPTLYGAYSRREVQLLSLDDMSDEATTPIGFLEACYASGGGQAVAAAFTDWERWCDE
                     VFDSHTAYPMLALFRSRQPGQNWLTALGVVLDAATICLAAVDSPRVGPAARLWRRSSR
                     LLQSLQGMASARLRLAEPDGAATEEAPFREAYERLRGLGVPLHPYERAWAEFRRLREA
                     YAPGLLTATRLLLVPLEFRHQTARLDITRSG"
     gene            complement(908..1297)
                     /locus_tag="VFO47_08715"
     CDS             complement(908..1297)
                     /locus_tag="VFO47_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET8788817.1"
                     /translation="MTSALSSSWPASRNEFWSWQVRGACRTAGPALFYSPEGERGSRK
                     RRRESAAKAICATCDVAEVCAAYALAWREPYGTWGGLSEADRETRYPELDPAQASIAY
                     RDALAAWYRRAELAGDPLDAPAVSLSG"
     gene            complement(1352..2335)
                     /locus_tag="VFO47_08720"
     CDS             complement(1352..2335)
                     /locus_tag="VFO47_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HET8788818.1"
                     /translation="MTPLHVTNGDIVADHLRRAGLAADALAWADVLHEGPVPAGLDDA
                     GLRRVRAEFLAGADGVDAEAVRRRFEDRDRALAAGRDGGYLLWFEADLYDQLQLAQIL
                     AMLRDTGVPPDRVRLVCIGEYPGIAHFGGLGELEPGQFPGLLETAAALTDGALDLGAA
                     AWAALRAADPGGLAAIAASRSPELRFLGEAFDRLGREYPSTRDGLSLTERRLLAAMHD
                     GSEPSAGAVFARLGEREARPFLADLFCFRILARLAGGRVPLVELDPPAGEVAAATRLR
                     LTAAGGRVLRGEADHVALNGIDRWVGGVHLHGQEARWRWDEGTESVTRAAA"
BASE COUNT          289 a          933 c         1006 g          327 t
ORIGIN      
        1 gtcgcgttgg ggcagcgggt cgagccggag ctgcgcgagg ggttcctggc cgtctacggg
       61 ccgttgtcgc tgctcggcct gctcgtgacc tgggtggtgc tgctcgtcat gggctggggc
      121 gcggtgtggt gggggctgcg ctcggccgtc accggtgtcg acggctacct cgacgccgtc
      181 tacttcgccg gcgtcgggtt cttcaccgtc ggcttcggcg acctcgtgcc caccggtggc
      241 ctggcccgcc tgctggtcct gctggaggcg ttcatggggc tggtcaccat ggccctggtc
      301 atcggctacc tgcccaccct gtacggcgcc tacagccgcc gggaggtcca gctcctctcc
      361 ctggacgaca tgtcggacga ggccaccacg ccgatcgggt tcctggaggc ctgctacgcc
      421 agcggcggcg gccaggcggt ggcggcggcg ttcaccgact gggagcggtg gtgcgacgag
      481 gtgttcgaca gccacaccgc ctacccgatg ctggccctgt tccgctcccg gcagccgggc
      541 cagaactggc tgacggcgct cggggtggtg ctcgacgccg ccaccatctg cctggccgcg
      601 gtcgacagcc cccgggtcgg gccggcggcg cggctgtggc ggcggtcgtc gaggctgctg
      661 cagagcctcc aggggatggc ctcggcccgg ctccggctgg ccgagccgga cggcgcggcc
      721 accgaggagg cgccgttccg ggaggcctac gagcgcctgc gcgggctcgg ggtgccgctt
      781 catccctacg agcgggcctg ggcggagttc cgccggctgc gggaggcgta tgcccccggg
      841 ctgctgacgg cgaccaggct gctgctggtg ccgctggagt tccggcacca gacggcccgg
      901 ctggacatca cccgctcagg ctgaccgccg gggcgtccag cgggtcgccg gccagttcgg
      961 cgcggcggta ccaggcggcc agggcgtcgc ggtaggcgat gctggcctgg gccgggtcca
     1021 gctccgggta gcgggtctcc cggtcggcct cggacagccc gccccaggtg ccgtacggct
     1081 cccgccaggc gagcgcgtag gcggcgcaga cctcggccac gtcgcaggtg gcgcagatcg
     1141 ccttggcggc gctctcgcgg cgccgtttgc gggagccacg ctcgccctcg ggcgagtaga
     1201 acaacgcggg cccggccgtc cggcaggcgc ccctgacctg ccaggaccag aactcgttgc
     1261 ggctggcggg ccagctggac gagagggcag aagtcacgcc tgggagcctg acgggtgccg
     1321 cccggccgcg catcacccgg gtcgggtgaa atcaggcggc cgcgcgggtg actgactcgg
     1381 tgccctcgtc ccagcgccag cgggcctcct gcccgtggag gtggacgcct ccgacccagc
     1441 ggtcgatgcc gttgagggcc acgtggtcgg cctcgccccg cagcacccgc ccaccggccg
     1501 cggtgaggcg caggcgggtg gcggcggcca cctccccggc cggcgggtcg agctcgacca
     1561 gcggcacccg gcccccggcc agccgggcca ggatccggaa gcagaacagg tcggccagga
     1621 acggccgtgc ctcccgctcc cccagccggg cgaacaccgc cccggcgctc ggctcgctcc
     1681 cgtcatgcat ggcggccagg aggcgccgct cggtcaggga gaggccatcg cgggtcgagg
     1741 ggtactcgcg gccgagccgg tcgaaggcct cgccgaggaa gcgcagctcg ggcgagcggc
     1801 tggccgcgat cgcggccagc ccgccggggt cggccgcccg cagcgccgcc caggcggcgg
     1861 cgcccaggtc gagggccccg tcggtcagcg cggcggcggt ctccagcagg ccggggaact
     1921 ggccgggctc gagctcgccc agcccgccga agtgggcgat gccggggtac tcgccgatgc
     1981 agaccagcct gacccggtcc ggcggcaccc cggtgtcgcg gagcatggcc aggatctggg
     2041 cgagctggag ctggtcgtac aggtcggcct cgaaccagag caggtacccg ccgtcgcggc
     2101 cggcggccag ggcgcggtcg cggtcctcga accggcgccg caccgcctcg gcgtcgacgc
     2161 cgtccgcccc ggcgaggaac tcggcccgga cccgccgcag cccggcgtcg tcgagcccgg
     2221 ccgggaccgg cccctcgtgc aggacgtcgg cccaggccag cgcgtccgca gccagcccgg
     2281 cgcggcgcag gtggtcggcg acgatgtcgc cgttggtcac gtgcagcggg gtcatgctcg
     2341 cctccggcgt ccggccgacg gcccgggatt gtacgcacca ccccggacag gaggccgtcc
     2401 ggggacaatg gaggagggac accgtcggca gggggagcgg gcaacatgcg ggcaggccgg
     2461 tcgcgcagcg gggtggtcct ggccgggctg gtggcgctgg tcctggtcgc cgccggagcg
     2521 gtcgcctggt tccagccgca gaagctgttc atcga
//
LOCUS       DASSWR010000519         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_810865,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000519 DASSWR010000000
VERSION     DASSWR010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810865"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFO47_08725"
     CDS             complement(<1..976)
                     /locus_tag="VFO47_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET8788819.1"
                     /translation="MAGYLVGLKLALLRNGLRRSNWAQQAALALGALAGLPLAFGGFL
                     LLALVPRAEPRAGLLLVEVVFLALFLGWGLFPLLSFAGDASLDPSRLALLPLRPGQLV
                     AGLSLAACVGVAPLCTLVALGGAVVGFGTLGPGLVLVVGAVLVQFALCIVGSRALLTA
                     LSRQLRSRKARDLVIVVVSVTALAFNLALQVGARLAERLERSDLEALRPLARVIGWLP
                     PGLAARALVDASQGRLLPAAAELAGAALAVVALGWWWWRSLDRVLTTAEPAARQRARQ
                     PAGLFPGPLRWLLPADQRGAVAAKELRYLVRHPRLRVAWMVSSLLGLGV"
     gene            complement(952..1749)
                     /locus_tag="VFO47_08730"
     CDS             complement(952..1749)
                     /locus_tag="VFO47_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788820.1"
                     /translation="MSTGEPTTGGRTPAALSVRGLRKAFGPKVAVAGIDLDIPHGCFL
                     GLVGPNGAGKTTTLGMVTGLLRPDAGLVVIDGVEVWRDPVEAKRRIGVLPEDLRLFDR
                     LTGSELLAYNGLLRGLPPPLVEARAAELLEVLGLADAAGTLVVDYSHGMRKKIALAAA
                     LLHAPRVLFLDEPFEAIDPVSARTIRGLLERYTAGGATVVFSSHVMELVERLCDRVGI
                     MHLGRLIAEGPTDQVRAGRSLEDAFVELVGASHAGAEELGWLGTWSA"
     gene            1789..>2407
                     /locus_tag="VFO47_08735"
     CDS             1789..>2407
                     /locus_tag="VFO47_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018468447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET8788821.1"
                     /translation="MDPSPAPEEAPGASPDLLDGLNDAQRLAVLATEGPTLIVAGAGS
                     GKTRVLTHRIAHLIKDRRVPPTSVLAITFTNKAAREMQERLRHLVGPVVKAMWVSTFH
                     SACVRILRRSGSHLGYTSTFSIYDEGDAERLMTQVCRQLDFDPKRLSARSMRHAVSGW
                     KDQLVDPEAAADRALGWFEKKAAEAYRLYQRRLVESNAMDFDDLIL"
BASE COUNT          362 a          939 c          857 g          249 t
ORIGIN      
        1 ccacgcccag cccgagcagg ctgctgacca tccaggcgac ccgcagccgc gggtgccgga
       61 ccagatagcg cagctccttg gcggcgaccg cgccccgctg gtcggctggg aggagccacc
      121 gcagcggccc cgggaacagc ccggccggct gccgggcccg ctgcctggcc gcgggctcgg
      181 cggtggtcag cacccggtcg aggctccgcc accaccacca gccgagcgcc accacggcca
      241 gggccgcgcc ggccagctcg gcggccgcgg gcaggagccg cccctggctg gcgtcgacca
      301 gggcccgggc ggccaggccg ggcggcagcc agccgatcac ccgggccagc ggccgcagcg
      361 cctccaggtc ggaccgctcc agccgctcgg ccagccgggc cccgacctgg agggccaggt
      421 tgaaggcgag cgccgtcacc gagacgacca cgatcaccag gtcgcgggcc ttgcgggagc
      481 ggagctggcg ggacagggcg gtgagcaggg cccgcgaccc gacgatgcac agggcgaact
      541 ggacgagcac ggcccccacc accagcacca acccgggacc gagggtgccg aacccgacca
      601 cggccccgcc cagggcgacc agggtgcaca gcggggccac gccgacgcag gcggccagcg
      661 acagcccggc caccagctgc ccggggcgta gcggcagcag ggccagccgg gaggggtcca
      721 ggctggcgtc gccggcgaag ctcagcagcg ggaacaggcc ccagccgagg aacagggcca
      781 ggaacaccac ctcgaccagc agcagcccgg cccggggctc ggcccgcggc accagggcga
      841 gcagcaggaa cccgccgaag gccaggggca gcccggccag ggcccccagg gcgagggcgg
      901 cctgctgggc ccagttgctg cgccgcagcc cgttgcggag cagagcgagc ttcaggccga
      961 ccaggtaccc agccaaccga gctcctcggc gccggcgtgg ctcgcgccca ccagctcgac
     1021 gaaggcgtct tcgaggctgc gcccggcccg cacctggtcg gtcggcccct cggcgatgag
     1081 gcggcccagg tgcatgatcc cgacccggtc gcagagccgc tcgaccagct ccatgacgtg
     1141 gctggagaag accaccgtgg ccccgccggc ggtgtagcgc tccagcagtc cgcggatggt
     1201 ccgggccgac accgggtcga tggcctcgaa cggctcgtcc aggaacagca cccggggggc
     1261 gtgcagcagg gccgcggcca gggcgatctt cttgcgcatc ccgtgggagt agtcgaccac
     1321 cagggtgccg gcggcgtcgg ccagccccag cacctccagc agctcggccg cccgcgcctc
     1381 caccagcggc ggcggcagcc cccgcagcag cccgttgtag gcgagcagct ccgacccggt
     1441 gagccggtcg aacaggcgca ggtcctcggg cagcaccccg atgcgccgct tggcttcgac
     1501 cgggtcgcgc cacacctcga ccccgtcgat caccaccagc ccggcgtcgg gccgcagcag
     1561 cccggtcacc atccccaggg tggtcgtctt cccggccccg ttcggcccca ccagcccgag
     1621 gaagcacccg tgggggatgt ccaggtcgat cccggccacc gccaccttgg gcccgaacgc
     1681 cttgcgcagc ccgcgcaccg acagcgcggc cggcgtgcgt ccaccagtcg tcggttcccc
     1741 tgtgctcacg ccggcaagcc tatcccgtac cgctcgtata ctgcgggcgt ggacccttcg
     1801 ccggcgcccg aggaagctcc gggtgcgtcc cctgacctgc tggacgggtt gaacgacgct
     1861 cagcggctgg ccgtgctggc caccgaggga ccgaccctga tcgtggccgg ggccgggtcg
     1921 ggcaagaccc gggtgctgac ccaccggatc gcccacctga tcaaggacag gcgggtgccg
     1981 ccgacctcgg tgctggccat caccttcacc aacaaggcgg ccagggagat gcaggagcgg
     2041 ctgcggcacc tggtcgggcc ggtcgtgaag gcgatgtggg tgtcgacctt ccactcggcc
     2101 tgcgtgcgga tcctgcgccg cagcggctcc cacctgggct acacctccac cttcagcatc
     2161 tacgacgagg gcgacgccga gcggctgatg acccaggtct gccggcagct cgacttcgac
     2221 cccaagcggc tctcggcccg ctccatgcgc cacgccgtga gcggctggaa ggaccagctg
     2281 gtcgaccccg aggccgccgc cgaccgggcc ctcggctggt tcgagaagaa ggccgccgag
     2341 gcctaccggc tctaccagcg ccgcctggtc gagtccaacg cgatggactt cgacgacctg
     2401 atcctgc
//
LOCUS       DASSWR010000520         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1008684, whole genome shotgun sequence.
ACCESSION   DASSWR010000520 DASSWR010000000
VERSION     DASSWR010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008684"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..846
                     /locus_tag="VFO47_08740"
     CDS             151..846
                     /locus_tag="VFO47_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8788822.1"
                     /translation="MRRRRALWRAAGARAGGRGRGSGVGRMPVAVLLDMGGVVIPTLF
                     ESVAVAGFPGGPFRGEAEYAAVERGEVPERDYWARLAERRPDLDVGALWRACSRVRVE
                     VRAAMGALAGRVRLVAFTNDMAHWFGPGWPGRFPELAGFDQVVEAARLGVLKPDPEAF
                     RRAAAEIGEDPDRCLFVDDLAVNLDGAAAVGMQTLLFEVRDPAGSIAALLACLGLDPD
                     PGPSRPGRVFSVR"
     gene            843..1343
                     /locus_tag="VFO47_08745"
     CDS             843..1343
                     /locus_tag="VFO47_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8788823.1"
                     /translation="MSARRVFATPLGRRRPAPDPERRLAALGLELPALRPKAGLYEGW
                     VRSGDLLFLSGQGADGWTGRLGADLTLDQGRRAARDCALNLLAQTRDALGSLDQVAQM
                     VKVTGFVACTPEFTGVPAVVDGASALLLDLFGDRGRHARSAVGVAALPLGFAVEVEMV
                     VRVSSG"
     gene            complement(1340..1603)
                     /locus_tag="VFO47_08750"
     CDS             complement(1340..1603)
                     /locus_tag="VFO47_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788824.1"
                     /translation="MPDSEVLPEDPHPLPPPTATFARDAERHLLDAPRHCPRCGASIA
                     GPNGLVVEYWAADDRVFYCWCRSCDWTGTVVQVDRHISHEAAD"
     gene            complement(1596..>2126)
                     /locus_tag="VFO47_08755"
     CDS             complement(1596..>2126)
                     /locus_tag="VFO47_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phthalate 4,5-dioxygenase"
                     /protein_id="HET8788825.1"
                     /translation="FPYCMRVGGAGIEQMQIRVPVDDTTTWFVLYTVHAPDGAPAPAQ
                     AAIPDYELPWRDEHGNHIVDYVEGQDIMAWVTQGPITDRTVEHLGKSDVGIAMLRKQF
                     KQQLAAVAAGRDPIAVVRDPAANDRIDLPCEKNKFGAGADFALQWMNLGFTRYSPQLD
                     DLLRLHTDAAEVRANA"
BASE COUNT          223 a          717 c          884 g          302 t
ORIGIN      
        1 ggtcctgtcc caggatcgcg ggcggctgtg gacagcccgc cgaaccccgc tccaaggtcg
       61 tctacgctcc cccttgggtc ggatgacctg tgcgcgaccg aacccggggc cgctcccgct
      121 gggcgagggg tcgacctgga cgactgggcg gtgcggcggc ggcgggcgct gtggcgggcc
      181 gccggggcca gggcgggggg ccggggccgg gggagcgggg tgggaaggat gccggtggcg
      241 gtgctgctgg acatgggcgg ggtggtgatc ccgaccctgt tcgagtcggt ggcggtggcg
      301 gggttccccg gggggccgtt ccggggagag gcggagtacg cggccgtcga gcggggggag
      361 gtgcccgaac gggactactg ggcgcggctg gccgagcggc ggcccgacct ggacgtgggg
      421 gcgctgtggc gggcctgctc gcgggtgcgg gtcgaggtgc gggcggcgat gggggcgctg
      481 gccgggcggg tccggctggt ggcgttcacc aacgacatgg cccactggtt cgggcccggc
      541 tggccggggc ggttcccgga gctggccggg ttcgaccagg tggtggaggc ggcccggctg
      601 ggggtgctca agcccgaccc ggaggcgttc cggcgggccg cggccgagat cggcgaggac
      661 cccgaccgct gcctgttcgt cgacgacctg gccgtcaacc tcgacggggc cgccgccgtc
      721 ggcatgcaga ccctgctgtt cgaggtccgc gaccccgccg ggtcgatcgc cgccctgctc
      781 gcgtgtctcg gcctcgaccc ggatcccggc ccttcccgcc ccggccgggt gttcagcgtc
      841 cgatgagcgc caggagggtg ttcgccaccc cgctcgggcg ccgccggccg gcgcccgacc
      901 cggagcggcg gctggccgcg ctcggcctgg agctgcccgc cctgcggccc aaggccgggc
      961 tctacgaggg ctgggtgcgg tccggcgacc tgctgttcct gtccggccag ggcgccgacg
     1021 gctggaccgg ccggctcggg gccgacctga ccctcgacca gggccgccgg gccgcccgcg
     1081 actgcgccct caacctgctc gcccagaccc gcgacgccct cggctccctg gaccaggtcg
     1141 cccagatggt gaaggtgacc gggttcgtgg cctgcactcc ggagttcacc ggcgtcccgg
     1201 ccgtggtcga cggcgcctcg gccctgctcc tggacctgtt cggcgaccgc ggccgccacg
     1261 cccgctccgc ggtcggggtg gccgccctgc cgctcgggtt cgcggtcgag gtcgagatgg
     1321 tggtccgggt gagctccggt tagtcggccg cctcgtggct gatgtggcgg tcgacctgga
     1381 ccacggtgcc ggtccagtcg caggagcggc accagcagta gaagacgcgg tcgtcggcgg
     1441 cccagtactc gaccaccagg ccgttggggc cggcgatgga ggcgccgcag cgggggcagt
     1501 ggcggggggc gtcgagcagg tggcgctcgg cgtcccgggc gaaggtggcc gtcggcgggg
     1561 gcagcgggtg cgggtcctcg ggcagcacct ccgaatcagg cattggcccg gacctcggcg
     1621 gcgtcggtgt ggaggcgcag caggtcgtcc agctgcggcg agtaccgggt gaagccgagg
     1681 ttcatccact ggagggcgaa gtcggccccg gcgccgaact tgttcttctc gcaggggagg
     1741 tcgatgcggt cgttggcggc cgggtcgcgg accacggcga tcgggtcgcg gccggcggcc
     1801 acggcggcca gctgctgctt gaactgcttg cggagcatgg cgatgccgac gtccgacttg
     1861 cccagatgct cgaccgtgcg gtcggtgatc ggaccctggg tgacccaggc catgatgtcc
     1921 tgaccctcga cgtagtcgac gatgtggttg ccgtgctcgt cgcgccaggg cagctcgtag
     1981 tcggggatgg cggcctgggc cggggccggg gcgccgtcgg gggcgtgcac cgtgtagagc
     2041 acgaaccagg tggtggtgtc gtcgaccggg acccggatct gcatctgctc gatcccggcg
     2101 cccccgaccc gcatgcagta ggggaa
//
LOCUS       DASSWR010000521         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_302117,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000521 DASSWR010000000
VERSION     DASSWR010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302117"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VFO47_08760"
     CDS             complement(<1..1068)
                     /locus_tag="VFO47_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8788826.1"
                     /translation="MGPLEGVRVVELAGIGPGPFCAMLLADLGAEVLAVDRPAAGGPG
                     WPALFARGRRRVAVDLKHPEGPGVVLDLVAGADALVEGFRPGVAERLGIGPEACLHRN
                     PRLVYGRVTGWGQDGPLARSAGHDIDYIAVAGALHPVGPAGGPPVPPLNLLGDFGGGG
                     MLLALGVVAALLEAGRSGRGQVVDAAMVDGAALLTTQLHELLAAGLWSDHRGANLLDG
                     AAPFYAVYETADGRHLAVGALEPQFWAELLDRLGLAADDLPAQLDRDGWPDLRERLAA
                     TFRTRTRDEWCALLEGTDACVAPVLSLLEAPAHPHNQARATFVDVGGVPQPAPAPRFS
                     RTPCAPPSPPPRGDDPAQVLAA"
     gene            1112..2005
                     /locus_tag="VFO47_08765"
     CDS             1112..2005
                     /locus_tag="VFO47_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8788827.1"
                     /translation="MADIRFDDRVAIVTGAGGGLGREHALLLASRGAKVVVNDLGGAT
                     DGTGASAGPAEQTVKEIEDLGGEAVVDTSTVATVEGGQAIVDTALDAFGRVDIVISNA
                     GILRDKAFHNLTPELLDPVLDVHLKGAFHVLRPAWVKMREAGYGRVLVTASNSGILGN
                     FGQSNYGAAKMGLVGLARVLAREGARYNIRANVLAPIARTRLTEELLGPLAPKLDPGL
                     VAPVAAWLVSEDCPVTGEIYSAGGGRVARFFVGLTPGYANPALTLEDVRDHFAEIRDE
                     TGYSVPGGVADELSSLVERLR"
     gene            complement(1945..2490)
                     /locus_tag="VFO47_08770"
     CDS             complement(1945..2490)
                     /locus_tag="VFO47_08770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8788828.1"
                     /translation="MITVRPATVADAPAMGRLHVRAWQAAYRGHMPGDYLDGLRASER
                     AAGWERVLRLERDRGAVLVAEQAGEVTGFANVGPAEDSADAGELYAINVDPAHWGTGA
                     GRALLLAAQAKLARLGNDETVLWVLPANARARRFYEIAGWVADGSERTMEVLGVVVPE
                     MRYRRRSTSEESSSATPPGTE"
     gene            complement(2532..>3039)
                     /locus_tag="VFO47_08775"
     CDS             complement(2532..>3039)
                     /locus_tag="VFO47_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET8788829.1"
                     /translation="YDRARSRRQATVAAASPGTRAERFGRAGAVPAPAPEDAEQELKR
                     LPVAKAAGIGLAQALALVPGTSRSGVTISAGLFQGVSRGTAARFSFLLSIPAILGAGI
                     LKLDELSGATETPAELVAGTVAAAVSGFVAVSFLLRLLRTRTLWPFIWYRLIAGGLFV
                     LLLATVRS"
BASE COUNT          396 a         1157 c         1154 g          332 t
ORIGIN      
        1 ggcggccagg acctgggcgg ggtcgtcgcc cctgggcggt ggagatgggg gagcgcaggg
       61 ggtgcggctg aagcgggggg cgggggcggg ctgggggacg ccgccgacgt cgacgaaggt
      121 ggcgcgggcc tggttgtggg ggtgggcggg ggcctccagg agcgacagca ccggggcgac
      181 gcaggcgtcg gtgccctcca gcagggcgca ccactcgtcg cgagtcctgg tccggaacgt
      241 cgccgccagc cgctcccgca ggtccggcca gccgtcgcgg tcgagctggg cgggcaggtc
      301 gtcggcggcc aggccgagcc ggtccagcag ctcggcccag aactgcggct ccagggcgcc
      361 aacggccagg tggcggccgt cggcggtctc gtagacggcg tagaacgggg ccgccccgtc
      421 gagcaggttg gcgccgcggt ggtcgctcca gagcccggcc gccagcagct cgtggagctg
      481 ggtggtgagc agggcggccc cgtcgaccat ggccgcgtcc accacctgcc cgcgccccga
      541 ccggcccgcc tcgagcaggg ccgccaccac cccgagggcg agcagcatgc cgccgccgcc
      601 gaagtcgccg agcaggttga gcggcggcac cggcggcccg ccggccggcc ccaccgggtg
      661 cagcgccccg gccacggcga tgtagtcgat gtcgtggccg gccgagcggg ccagcgggcc
      721 gtcctggccc cagccggtga cgcgcccgta gaccaggcgc gggttgcggt gaaggcacgc
      781 ctcgggcccg atcccgagcc gctccgccac ccccggccgg aacccctcga ccagggcgtc
      841 ggccccggcg accaggtcga gcaccacccc cgggccctcg ggatgcttga ggtcgacggc
      901 gacccggcgc cgtccccggg cgaacagcgc cggccagccg ggcccacccg ccgccggacg
      961 gtccacggcc agcacctcgg cgcccaggtc ggccaggagc atggcgcaga acggccccgg
     1021 cccgatcccg gccagctcga cgacccgcac tccctcgagg ggacccatgc ccgtatcgta
     1081 gggtccggtg gcgcgacacg ggaggcgatc gatggccgac atccgtttcg acgacagggt
     1141 ggcgatcgtg accggggccg gcgggggcct gggccgtgag cacgccctgc tgctggcctc
     1201 gcggggcgcc aaggtggtcg tcaacgacct cggcggcgcc accgacggca ccggcgcgtc
     1261 ggccggcccg gccgagcaga cggtcaagga gatcgaggac ctgggcgggg aggccgtcgt
     1321 cgacaccagc acggtggcca cggtcgaggg cgggcaggcg atcgtcgaca ccgccctcga
     1381 cgccttcggc cgggtcgaca tcgtcatcag caacgccggc atcctgcgcg acaaggcgtt
     1441 ccacaacctg accccggagc tgctcgaccc cgtgctcgac gtgcacctca agggggcctt
     1501 ccacgtgctc cggccggcct gggtgaagat gcgcgaggcc ggctacggcc gcgtcctggt
     1561 caccgcctcc aactcgggca tcctcggcaa cttcggccag agcaactacg gcgccgccaa
     1621 gatgggcctg gtcggcctgg cccgggtgct cgcccgggag ggcgcccgct acaacatcag
     1681 ggccaacgtg ctcgccccga tcgcccgcac ccgcctgacc gaggagctgc tgggcccgct
     1741 cgcccccaag ctcgacccgg gcctggtcgc gcccgtggcc gcctggctgg tctccgagga
     1801 ctgcccggtg acgggcgaga tctactcggc cgggggcggg cgggtcgccc gcttcttcgt
     1861 cggcctcacc cctggctacg ccaacccggc cctcaccctg gaggacgtgc gcgaccactt
     1921 cgccgagatc cgcgacgaga ccggctactc ggtgccgggc ggcgtcgccg acgagctctc
     1981 ctcgctggtc gagcgcctcc ggtagcgcat ctcgggaacg acgacgccga gcacctccat
     2041 ggtccgctcg ctgccgtcgg cgacccagcc ggcgatctcg tagaagcggc gggcgcgggc
     2101 attggcgggc aggacccaca gcaccgtctc gtcattgccc agcctggcca gcttggcctg
     2161 ggcggccagg agcagggccc ggccggcgcc cgtcccccag tgggcggggt cgacgttgat
     2221 ggcgtacagc tcgcctgcgt ccgcggagtc ctcggccggg ccgacgttgg cgaacccggt
     2281 cacctcgccg gcctgctcgg ccaccaggac ggcgccccgg tcccgctcca agcgcagcac
     2341 gcgctcccag ccggcggcac gctcggaggc gcggaggccg tcgaggtagt cgccgggcat
     2401 gtggccgcgg taggcggcct gccaggcgcg gacgtgcagc cggcccatgg ccggcgcgtc
     2461 cgcgaccgtg gccggtcgga ccgtgatcat ggccaagagc gtacccgggc ctcccggtcc
     2521 ggtcgccgga tttacgaccg gacggtggcc agcagcagca cgaacagccc gccggcgatc
     2581 agccggtacc agatgaacgg ccacagggtc cgggtccgca gcaggcggag caggaacgac
     2641 acggccacga accccgagac ggcggcggcg acggtgccgg cgaccagctc ggccggggtc
     2701 tcggtggcac ccgacagctc gtccagcttg aggatgccgg cgcccaggat ggccgggatc
     2761 gacagcagga acgagaagcg ggccgccgtg cccctggaca cgccctggaa cagcccggcc
     2821 gagatcgtca ccccggagcg gctggtcccg gggaccaggg cgagggcctg ggccaggccg
     2881 atgccggccg ccttggccac cggcagccgc ttcagctcct gctcggcgtc ctcgggcgcc
     2941 ggggcgggca ccgccccggc ccgcccgaac cgctcggccc gggtccccgg gctcgccgcc
     3001 gcgaccgtgg cctgccgccg gctgcgggcc cggtcgtac
//
LOCUS       DASSWR010000522         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_753556,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000522 DASSWR010000000
VERSION     DASSWR010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753556"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..276)
                     /locus_tag="VFO47_08780"
     CDS             complement(40..276)
                     /locus_tag="VFO47_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET8788830.1"
                     /translation="MRDVVVYLARALVDDPDRVTVEELPGARGVTYEVRTAPGDVGKL
                     IGRGGRTVKALRRVVKAVAPKGHRVEVEVLAEGE"
     gene            complement(388..627)
                     /gene="rpsP"
                     /locus_tag="VFO47_08785"
                     /pseudo
     CDS             complement(388..627)
                     /gene="rpsP"
                     /locus_tag="VFO47_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(723..2054)
                     /gene="ffh"
                     /locus_tag="VFO47_08790"
     CDS             complement(723..2054)
                     /gene="ffh"
                     /locus_tag="VFO47_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET8788831.1"
                     /translation="MFDTLSDRLQDTFARLRGHGRLSEAQVDAALREVRLALLEADVN
                     FKVVKDFIARVRERAVGEEVTRSLTPAQQVVKIVHEELVVTLGERNVPLQWSPRPPTV
                     LLMAGLQGSGKTTTAAKLARWLSGKGRKPLLVACDLQRPAAVKQLQVLGEQVKVPVFA
                     PHPGGPDADPVPVALGARDEAERTGRDVVIVDTAGRLAIDAEMMAQAAAIREAVQPTE
                     VLLVVDAMTGQDAVATAQAFQEGVDVTGFVLTKLDGDARGGAALSIRSVTGRPIKFAA
                     TGEKLADFDAFHPDRLAGRILGMGDVLTLIERAEEQIDREQAVQMERKLREATFTLED
                     FLDQLQQVKKMGPLSQVLGMLPGMSQQLKGVEVDDRDVARVEAIIRSMTPGERNNPKI
                     LNGSRRARIARGSGTTTQQVNALLKQFEEARRMMKQLSGMTPKKLRRMGLR"
BASE COUNT          278 a          810 c          822 g          359 t
ORIGIN      
        1 gcacgcgccc gacgaccagg cgctcgggag cggacatgct cactcgccct cggcgaggac
       61 ctcgacctcg acccggtggc ccttgggggc gacggccttg acgacccggc gcagggcctt
      121 gacggtgcgg ccgccccgcc cgatcagctt gccgacgtcg cccggggcgg tccggacctc
      181 gtaggtgacg ccgcgggcgc cgggcagctc ctcgacggtg acccggtccg ggtcgtcgac
      241 cagcgccctg gccaggtaga cgacgacgtc acgcatggcc ggctacttct tgcccttggc
      301 cttggccttg ggcttcttgg gctcggcctt ggccttggcc acggtggccc gcaggtcctt
      361 ggcggcctgc tggcgctcgg ccatgccctc gactccggcg atggccagca gcttggccac
      421 ggcctcggtc ggctgggcgc ccttggccag ccaggcggac gccttctcgg tgtcgatctc
      481 gaccttggac ggctcgttgt tggggtggta gacgccgagg ttctcgagga agcgcccgtc
      541 gcgggggctg cgcgagtcgg cgacgacgac ccggtagaac ggggccttct tggcgcccat
      601 gcgcatcagg cggatcttca cggccatggt gtggagttgc tccgatctcg gggcggcccg
      661 cccgtcccgg gaggtctgcc gttccccgtc gtgtgcctgt ctggctccgg gaccgaggca
      721 gtctagcgca ggcccatccg gcgcagcttc ttcggggtca tgcccgacag ctgcttcatc
      781 atccggcggg cctcctcgaa ctgcttgagg agggcgttga cctgctgggt ggtggtgccc
      841 gagccgcggg cgatgcgggc ccggcgcgag ccgttgagga tcttggggtt gttgcgctcg
      901 cccggggtca tggagcggat gatcgcctcc acccgggcca cgtcgcggtc gtcgacctcg
      961 acgcccttga gctgctggct catgcccggg agcatgccaa ggacctggct gaggggaccc
     1021 atcttcttga cctgctggag ctggtcgagg aagtcctcga gggtgaaggt ggcctcgcgg
     1081 agcttgcgct ccatctgcac ggcctgctcg cggtcgatct gctcctcggc ccgctcgatc
     1141 agggtgagca cgtcgcccat gcccaggatc cgcccggcca ggcggtcggg gtggaaggcg
     1201 tcgaagtcgg ccagcttctc gccggtggcg gcgaacttga tcggccgccc ggtcaccgac
     1261 cggatcgaca gggcggcgcc gccccgggcg tcgccgtcga gcttggtcag gacgaacccg
     1321 gtgacgtcca cgccctcctg gaaggcctgg gccgtggcca ccgcgtcctg gccggtcatg
     1381 gcgtcgacca ccagcagcac ctcggtcggc tggacggcct cgcggatggc ggccgcctgg
     1441 gccatcatct cggcgtcgat ggccagccgg ccggcggtgt cgacgatcac cacgtcgcgg
     1501 ccggtccgct cggcctcgtc gcgggcgccg agagcgaccg gcaccgggtc ggcgtccggc
     1561 ccccccgggt ggggtgcgaa caccggcacc ttgacctgct cccccagcac ctggagctgc
     1621 ttcacggccg ccgggcgctg caggtcgcag gcgaccagca ggggcttgcg gcccttgccg
     1681 gacagccagc gggccagctt ggccgcggtg gtggtcttgc ccgagccctg caggccggcc
     1741 atcagcagca ccgtcggcgg ccgcggcgac cactggagcg gcacgttgcg ctcgcccagg
     1801 gtgacgacca gctcctcgtg gacgatcttg accacctgct gggccggggt gaggctgcgg
     1861 gtgacctcct cccccaccgc ccgctcgcgc acccgggcga tgaagtcctt caccaccttg
     1921 aagttgacgt ccgcctccag cagggccagc cggacctcgc gcagggccgc gtccacctgg
     1981 gcctcggaca gccgcccatg cccccgcagg cgggcgaagg tgtcctggag ccggtcggag
     2041 agggtgtcga acacgcgggg gcttcctttg cgttgatggc ggctgcgtcc aagcatagcc
     2101 gtccccaggc aacctcaacg gcgcccggct tcgtcatccc cccaggaggg aggatggacc
     2161 tggacggcgg ccgcggaacc tcggcttcga ggaagcctag gctggctatc ctgaccggca
     2221 gcagacgccg catcgaagga gccgttggtg gagcacaccc atctgggac
//
LOCUS       DASSWR010000523         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1319369, whole genome shotgun sequence.
ACCESSION   DASSWR010000523 DASSWR010000000
VERSION     DASSWR010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319369"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFO47_08795"
     CDS             <1..135
                     /locus_tag="VFO47_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8788832.1"
                     /translation="GGTNREIGAQLFLSRRTIDYHLRNVFVKLGVSSRAELIRLQLDR
                     "
     gene            208..714
                     /locus_tag="VFO47_08800"
     CDS             208..714
                     /locus_tag="VFO47_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HET8788833.1"
                     /translation="MSVSVAKLFGLEGDGWMRHANPVSVWTRFAVLPLLALAIWSREW
                     IGWWSLAAVALALVFMMVNPLLFPPARSTRNWASKGVLGERIWSDRSKVELPPQFRGS
                     GVTYATYAFQVAGLAIMVYGLVELDLLAVVAGLLVTQAAKAWFIDRMVLLFDDMKARV
                     PEYAAWDY"
     gene            746..2944
                     /locus_tag="VFO47_08805"
     CDS             746..2944
                     /locus_tag="VFO47_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8788834.1"
                     /translation="MDSSPDHAIGTARDAAPPGSRRGRRRRRPTGEPPPLPRHLEVTG
                     FGWMIVAVGLITLTLLLFTAGRYGRGISLTVIDNRVVEALANLRTPGLTSLMRGLSGL
                     LGSLWTIKALAWSTMIVLIVYKRFRHLIVGLISFQVVGLVLVGLSAAVRRPRPFGVEI
                     EGAWAGWAMPSRPVAFLSAILVTMLYSLVPKGRWRQAGKWAATGLVTLLALARMYLGV
                     DTPSDVLVGAAIGVTIPLLAYRWFTPNEVFPVSYRRGRTAHLDVGGERGQAIRRALAD
                     QLGLEIEDVKPFGLSGSAGSTPLRITVAGDPPTVLFGKLYARSHLRSDRWYKLGRELL
                     YGRLEDEKPFHTVRRLVQQEDYALHKLHQVGLPTPRPYGFVELTPDREYLLVFEFFDG
                     AVELGEAEVDERVIDDGLRIIRKLWEAGLAHRDIKPANLLVRDGRMLLIDVAFVQARP
                     SPWRQAVDLANMMLCLALRSNPELVYQRAQRQFSVEEITEGFAAARGLALPSQLRRML
                     RAQGRDLHAEFVRLLPTPPQPVSIQRWSWRRVGLMAATAVLLVLAIAAQLSLTNNDAA
                     TKTPLKIGSLPCTDLEPLWLQAQAVPSASLVPCVRDLPVGWSLANVAVNDGRSVISLH
                     HDRTGGQAMEARLTAGCEIAGVAEQPSGQPGVRRYQLVTRQGPVFQAVRFDVFSGGCL
                     ITRIRALPTSRAEVMAAAPGILGFASREELSQALERRSDGRLHLDPAGGR"
BASE COUNT          366 a         1098 c         1035 g          445 t
ORIGIN      
        1 ggcgggacca accgcgagat cggcgcccag ctgttcctca gccgccgcac catcgactac
       61 cacctgcgca acgtcttcgt gaagctcggg gtctcctccc gggccgagct gatccggctc
      121 cagctcgacc ggtgacccta ccgatacgac ggggccgggc ggcggcggat gctgggggcg
      181 aggcacgccc cacgcaggag gtccccgatg agcgtctcgg tcgccaagct gttcggcctc
      241 gagggcgacg gctggatgcg ccacgccaac ccggtgagcg tctggacccg gttcgccgtc
      301 ctgccgctgc tggccctggc catctggagc cgggagtgga tcggctggtg gtcgctggcc
      361 gcggtcgccc tggccctggt gttcatgatg gtcaacccgc tgctgttccc cccggcccgc
      421 tcgacccgca actgggcctc caagggcgtg ctcggcgagc gcatctggag cgaccggagc
      481 aaggtcgagc tcccgcccca gttccggggc tcgggggtga cgtacgcgac ctacgccttc
      541 caggtggccg gcctggccat catggtgtac gggctggtcg agctggacct gctggccgtg
      601 gtcgcggggc tgctcgtcac ccaggcggcc aaggcctggt tcatcgaccg catggtgctg
      661 ctgttcgacg acatgaaggc ccgcgtcccc gagtacgccg cctgggacta ctgagcgggc
      721 accgaaggtt tgccatactc cgttcatgga cagcagccca gaccacgcca tcgggaccgc
      781 ccgtgacgcg gccccgccgg gcagccggcg gggccggcgc cggcggcggc cgaccgggga
      841 gccgccgccg cttccgcgcc acctggaggt caccggcttc ggctggatga tcgtggccgt
      901 cgggctgatc accctcacgt tgctgctgtt caccgccggc cgctacggcc gcggcatctc
      961 cctcacggtg atcgacaacc gggtggtgga ggcgctggcc aacctgcgca cccccgggct
     1021 gaccagcctc atgcgaggac tgagcgggct gctcgggtcc ctgtggacga tcaaggcgct
     1081 ggcctggtcg accatgatcg tgctgatcgt ctacaagcgc ttccgccacc tgatcgtcgg
     1141 gctgatctcc ttccaggtgg tgggcctggt cctggtcggg ctgtcggcgg cggtgcggcg
     1201 gccgcggccg ttcggggtgg agatcgaggg ggcctgggcc ggctgggcga tgccgtcgcg
     1261 gccggtcgcc ttcctctccg ccatcctggt cacgatgctg tacagcctgg tccccaaggg
     1321 ccgctggcgg caggcgggca agtgggccgc caccgggctg gtcaccctgc tcgccctggc
     1381 ccgcatgtac ctgggggtcg acacccccag cgacgtgctc gtcggggcgg ccatcggcgt
     1441 caccatcccg ctgctcgcct accgctggtt cacccccaac gaggtcttcc cggtcagcta
     1501 ccggcggggc cgcaccgccc acctggacgt cggcggggag cgcggccagg ccatccggcg
     1561 ggccctggcc gaccagctgg gcctggagat cgaggacgtc aagccgttcg gcctgtccgg
     1621 ctcggccggc tcgaccccgc tgcggatcac cgtcgcgggc gaccctccga cggtcctgtt
     1681 cggcaagctg tacgcccgca gccacctgcg ctcggaccgc tggtacaagc tcggccggga
     1741 gctgctctac gggcgcctgg aggacgagaa accgttccac accgtccgcc ggctggtcca
     1801 acaagaggac tacgccctgc acaagctcca ccaggtcggc ctgccgacgc cccgccccta
     1861 cgggttcgtc gagctcaccc ccgaccgcga gtacctgctg gtgtttgagt tcttcgacgg
     1921 ggcggtggag ctgggcgagg ccgaggtcga cgagcgggtg atcgacgacg gcctgcggat
     1981 catccgcaag ctgtgggagg ccgggctggc ccatcgcgac atcaagccgg ccaacctgct
     2041 ggtccgcgac ggccgcatgc tcctgatcga cgtcgccttc gtccaggccc gacccagccc
     2101 gtggcgccag gccgtcgacc tggccaacat gatgctgtgt ctggccctgc gctcgaaccc
     2161 ggagctggtc taccagcggg cccagcggca gttcagcgtg gaggagatca ccgaggggtt
     2221 cgcggccgcc cgcggcctgg ccctgccgtc gcagctgcgc cggatgctca gggctcaggg
     2281 ccgcgacctc cacgccgagt tcgtccgcct gctgccgacc ccgccccagc cggtgtcgat
     2341 ccagcggtgg agctggcggc gggtcgggct catggccgcc acggcggtcc tgctggtgct
     2401 cgcgatcgcc gcccagctct ccttgaccaa caacgacgcc gccaccaaga ccccgctcaa
     2461 gatcggcagc ctgccctgca ccgacctgga gccgctgtgg ctgcaggccc aggccgtgcc
     2521 gtcggcgtcg ctggtgccgt gcgtgcgcga cctgcccgtc ggctggtccc tggccaacgt
     2581 ggccgtcaac gacggccggt cggtgatctc cctgcaccac gaccggaccg gcggccaggc
     2641 gatggaggcc cggctgaccg ccggctgcga gatcgccggg gtggccgagc agccctcggg
     2701 ccagcctggg gtccggcgct accagttggt cacccgccag gggccggtct tccaggccgt
     2761 gcgcttcgac gtcttctcgg gcggctgcct gatcacccgc atccgggcgc tccccaccag
     2821 ccgggccgag gtcatggccg cggcccccgg catcctcggc ttcgcctccc gcgaggagct
     2881 cagccaggcg ctggagcggc gctccgacgg gcggctccac ctcgacccgg ccggcggccg
     2941 ctag
//
LOCUS       DASSWR010000524         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_102390,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000524 DASSWR010000000
VERSION     DASSWR010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102390"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1671)
                     /gene="miaB"
                     /locus_tag="VFO47_08810"
     CDS             complement(244..1671)
                     /gene="miaB"
                     /locus_tag="VFO47_08810"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET8788835.1"
                     /translation="MSERYLIRTFGCQMNEHDSERLAGLLEAEGMERAAGLDDADLVV
                     LNTCAVRENADNKLYGTLGHLATTKARRALTIAVGGCLAQKDQGRVLERAPWVDVVFG
                     THNLHSLPVLVRRAREQGAAQAEFFDEMQRFPSALPARRASEWSAWVAISVGCNNTCT
                     FCIVPKLRGKERSRRVGDVVAEVQALADAGVLEVSLLGQNVNSYGTDLPGHRQLFGDL
                     LRALDGVEGLERLRFTSPNPKDFRDDVVEAMAGCRPLCEHVHFPLQSGSDRVLRLMRR
                     GYRRRRYLEILASLRAAIPGVAFSTDIIVGFPGETEADFADTLDLVEQARFDSAFTFQ
                     YSPRPGTEAASMPDQVPADVVKDRFQRLLQLQERVSLEANRALLGTEVELLVEQSTSK
                     TNPTRMSGRTRTNKLCHFPAPAVTGVVAVAPGDLVTVRVEQAAPHHLVGGPVLAHRPW
                     RPAAGSCGAAPRPLPAGAVPLPLVG"
     gene            complement(1705..>2079)
                     /locus_tag="VFO47_08815"
     CDS             complement(1705..>2079)
                     /locus_tag="VFO47_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET8788836.1"
                     /translation="PEVVHAIEAHHGEVEQRTIEAVLTQAADAISGGRPGARRETLES
                     YVKRLERLEEIAGSYPGVDKVFAMQAGREVRIMVKPEDVDDVAAQVLARDMAKRVEQE
                     LQYPGQIKITVVREIRSIEYAK"
BASE COUNT          267 a          702 c          825 g          285 t
ORIGIN      
        1 gtcggcagcg tcgaggaccc tggacagggc ctcgtggcag gcggcccgca gtgggtcgag
       61 ctcggccgcg gcgccgccgg ccagctcggg cacgagcagg ggcgggtgcg gcaggacggc
      121 cgccacgacc gggtgacgac cggcgaccgg gtgacgaccg ggaaccggct gacgatcggc
      181 gaccggctgt tcccggtgag cggcgggctg cgggtcccgg ccctcacgcg gcatgggcga
      241 ccctcagccg accagcggca gggggaccgc cccggcgggc agcggccgcg gcgccgcccc
      301 gcacgacccg gcggcaggtc gccatggccg gtgggccagc accggccccc cgaccaggtg
      361 atggggggcc gcctgctcga cccggaccgt caccaggtcc ccgggcgcca ccgcgacgac
      421 acccgtgacc gccggggccg ggaagtggca gagcttgttg gtccgggtgc ggcccgacat
      481 gcgggtgggg ttggtcttgg aggtggactg ctcgaccagg agctcgacct cggtgccgag
      541 cagggcccgg ttggcctcca ggctgacgcg ctcctggagc tggaggaggc gctggaagcg
      601 gtccttgacc acgtcggcgg gcacctggtc gggcatggag gcggcctcgg tgccggggcg
      661 gggcgagtac tggaaggtga aggccgagtc gaaccgggcc tgctccacca ggtcgagggt
      721 gtcggcgaag tcggcctcgg tctcgcccgg gaacccgacg atgatgtcgg tggagaaggc
      781 gaccccgggg atggcggcgc gcaggctggc caggatctcg aggtagcggc gcctgcggta
      841 gccgcggcgc atcagccgca ggacccggtc ggagcccgac tggagcggga agtggacgtg
      901 ctcgcacagg ggccggcagc cggccatggc ctcgaccacg tcgtcgcgga agtccttggg
      961 gttggggctg gtgaagcgga gacgctccag gccctcgacc ccgtccagag cccggagcag
     1021 gtcgccgaac agctggcggt gcccgggcag gtcggtgccg taggagttga cgttctggcc
     1081 gagcaggctg acctcgagca cgccggcgtc ggccagggcc tggacctcgg cgaccacgtc
     1141 gccgacccgg cggctgcgct ccttgccgcg cagcttgggg acgatgcaga aggtgcaggt
     1201 gttgttgcag ccgacgctga tggcgaccca ggccgaccac tcgctggccc gccgggccgg
     1261 gagggcggag gggaagcgct gcatctcgtc gaagaactcg gcctgggcgg cgccctgctc
     1321 gcgggcccgg cgcaccagga cgggcaggct gtgcaggttg tgggtgccga agaccacgtc
     1381 gacccagggg gcgcgctcca gcacccggcc ctggtccttc tgggcgaggc agccgccgac
     1441 ggcgatggtc agcgcgcgcc tggccttggt ggtggccagg tggccgaggg tgccgtagag
     1501 cttgttgtcg gcgttctcgc ggaccgcgca ggtgttgagc acgaccaggt cggcgtcgtc
     1561 cagcccggcg gcacgctcca tgccctcggc ctcgagcagc ccggccagcc gctcggagtc
     1621 gtgctcgttc atctggcacc cgaaggtgcg gatcaggtat cgctcactca tcgcgccgga
     1681 tggtactccg ccggcctggt catctcactt ggcgtactcg atgctgcgga tctcgcggac
     1741 cacggtgatc ttgatctggc ccgggtactg gagctcctgc tcgacccgct tggccatgtc
     1801 gcgggccagc acctgggcgg cgacgtcgtc gacgtcctcg ggcttgacca tgatccggac
     1861 ctcgcggccg gcctgcatgg cgaagacctt gtcgacgccc gggtagctgc cggcgatctc
     1921 ctcgaggcgc tccaggcgct tgacgtagga ctcgagcgtc tcccggcggg ccccggggcg
     1981 gccgcccgag atggcgtcgg cggcctgggt gagcacggcc tcgatggtgc gctgctcgac
     2041 ctcgccgtgg tgggcctcga tggcgtggac gacctcggg
//
LOCUS       DASSWR010000525         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_724823,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000525 DASSWR010000000
VERSION     DASSWR010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724823"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFO47_08820"
     CDS             complement(<1..484)
                     /locus_tag="VFO47_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8788837.1"
                     /translation="MHTAGVDLGGTKVQGVVVGDDGAKLGEARGKTPAGDGAAAVVAE
                     IAKVVKAAARDAKVAPHKLAGVGIGSPGRVDAATGELFGAANVFGAVDRVPLGAMVAD
                     AVGVERVVVDNDVVVATLAEHRLGAGRGFDDLLPVFAGSGVGGALVLGGRLRAGAHGA
                     A"
     gene            619..1371
                     /locus_tag="VFO47_08825"
     CDS             619..1371
                     /locus_tag="VFO47_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788838.1"
                     /translation="MSAAIVPGEETLAARAVQVTKIYGQGQTEVRALDNVSVEFPSGR
                     FTAIMGPSGSGKSTLMHCLAGLDRITSGQIFIGDVELSKLSEKQLTLLRRDKVGFVFQ
                     AFNLLPTLTAAENVELPLAIAGRKPDRAWVDQVVDAVGLRERLAHKPAELSGGQQQRV
                     AAARALASKPAIVFADEPTGNLDSAAGAELLGFMRRSVDEYGQTIVMVTHDPNSAAFS
                     DHVLFLADGRIVDEMANPTAEKVLDLMKKLGG"
     gene            1380..>3203
                     /locus_tag="VFO47_08830"
     CDS             1380..>3203
                     /locus_tag="VFO47_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8788839.1"
                     /translation="MRKATLKGLLAHKFRLAATALAIVLGVSFVAGTYVLTDTITASF
                     DSLFKQVTQGIDVAVRSEETFGGFEGETRDPMPASLLERVKAVGGVRVAEGNVTGYAQ
                     LVGKDGKAVTTSGAPSLGVSFNKDPQFSAGSTVRSGRLPSGPTEMAIDAKTAEDTGYQ
                     VGDRAKVLFQGPAREFTVSGVIGFGEADNLGGARLVGFDLATAQEVMNREGRFDEIDV
                     AAVEGVTPEELRDRVRAAIADPKYEVLTGTELAEDSAAQINDTIGRFLGTALLAFAGV
                     ALLVGAFLIFNTFTIIVAQRTRELALLRCLGASRRQVMTSVLVESVIVAVVASLVGLG
                     LGLLIASGLRALLSGFLGADIPATGIQFQPRTVVVSLLVGVVVTVLAALLPALKATRV
                     PPVAALQPETAFAPTGFRKRRIVLGVLVTAVGVAALLAGLFRSEGNQLVNVASGAVIV
                     FFGVAILSPLIARPLARVIGWPFARAFRLPGTLARQNAMRNPRRTASTAAALMIGLAL
                     VAFVSIFAASIKASTSKTLEDTVSADYILFNDNFQPFSPEAAERLAEQPELAAVAGMR
                     ISPWKLNGAGKSLAGIDPAVYQQVVRTETTAGSLNDLASGGL"
BASE COUNT          441 a         1230 c         1081 g          451 t
ORIGIN      
        1 cggcggctcc gtgggcgccg gcccgcaacc gccccccgag caccagggcg ccgcccaccc
       61 ccgacccggc gaacaccggg agcaggtcgt cgaagccccg gccggccccg aggcggtgct
      121 cggccagggt ggccacgacc acgtcgttgt cgaccaccac ccgctcgacc ccgaccgcgt
      181 cggcgaccat cgcccccagc ggcacccggt cgacggcccc gaagacgttg gccgccccga
      241 acagctcccc ggtggccgcg tcgacccggc cgggggagcc gatcccgacc ccggccagct
      301 tatgcggcgc caccttggcg tccctggccg ccgccttgac caccttggcg atctcggcca
      361 ccaccgccgc cgccccgtcc ccggccgggg tcttgcccct ggcctcgccg agcttggcgc
      421 cgtcgtcgcc gacgaccacc ccctggacct tggtcccgcc taggtcgacc cctgccgtat
      481 gcatgacccg gatgatgcca gcatcctccg aaaggtggag agccgaatcg gcgccgtggc
      541 cgacgaatcg gcttgcttgg tcgcgtaggc tcgccacgag accaagcaac gatccatgac
      601 gagaagggga gccgtcccat gtcagctgcc atcgtgcccg gcgaggagac cctggccgcg
      661 cgggccgtcc aggtgaccaa gatctacggg cagggccaga ccgaggtgcg ggccctcgac
      721 aacgtctccg tcgagttccc cagcggccgc ttcacggcca tcatgggccc gtccggctcg
      781 ggcaagtcga ccctgatgca ctgccttgcc gggctggacc ggatcacctc ggggcagatc
      841 ttcatcggcg acgtcgagct cagcaagctg tccgagaagc agctcaccct gctccgccgg
      901 gacaaggtcg gcttcgtgtt ccaggccttc aacctgctcc cgaccctgac cgcggccgag
      961 aacgtcgagc tgcccctggc catcgccggc cgcaagccgg accgggcctg ggtcgaccag
     1021 gtggtcgacg ccgtcggact gcgcgagcgc ctggctcaca agccggccga gctgtccggc
     1081 ggccagcagc agcgggtggc cgcggccagg gcgctggcct ccaagccggc gatcgtgttc
     1141 gccgacgagc ccaccggcaa cctcgactcg gccgccggcg ccgagctgct cggcttcatg
     1201 cgccgctcgg tcgacgagta cggccagacg atcgtgatgg tcacccacga ccccaactcg
     1261 gccgccttct ccgaccacgt gctgttcctg gccgacggcc gcatcgtcga cgagatggcc
     1321 aacccgaccg ccgagaaggt gctcgacctc atgaagaagc tggggggctg acggcggcca
     1381 tgcgcaaggc gacgctcaag ggcctcctgg cccacaagtt ccggctggcc gcgaccgccc
     1441 tggccatcgt gctcggggtc tccttcgtgg ccggcaccta cgtgctcacc gacaccatca
     1501 cggccagctt cgactcgctg ttcaagcagg tcacccaggg catcgacgtg gctgtccgct
     1561 ccgaggagac cttcggcggc ttcgagggtg agacccgcga ccccatgccg gcctccctcc
     1621 tggagcgggt caaggccgtg ggcggcgtca gggtggccga gggcaacgtc accggctacg
     1681 ctcagctcgt cggcaaggac ggcaaggcgg tgaccaccag cggcgccccc agcctgggtg
     1741 tgtcgttcaa caaggacccc cagttctcgg ccggctcgac cgtccgctca gggcggttgc
     1801 cgagcgggcc gaccgagatg gccatcgacg ccaagacggc tgaggacacc ggctaccagg
     1861 tcggcgaccg ggccaaggtg ctgttccagg gcccggcccg cgagttcacg gtcagcggcg
     1921 tgatcggctt cggggaggcc gacaacctcg gcggggcccg cctggtcggc ttcgacctgg
     1981 ccaccgccca ggaggtgatg aaccgcgagg gccgcttcga cgagatcgac gtcgccgccg
     2041 tggagggcgt cacccccgag gaactgcgcg accgggtccg ggcggccatc gccgacccca
     2101 agtacgaggt gctcaccggc acagagctgg ccgaggactc ggccgcccag atcaacgaca
     2161 ccatcgggcg gttcctgggc acggccctgc tcgccttcgc cggcgtggcc ctgctggtgg
     2221 gcgcgtttct gatcttcaac accttcacga tcatcgtcgc ccagcgcacc cgcgagctgg
     2281 ccctgctgcg ctgcctgggg gcgagccgcc gccaggtgat gacctcggtg ctggtcgagt
     2341 ccgtgatcgt ggccgtggtg gcgtcgctgg tcggcctcgg cctcggcctg ctgatcgcca
     2401 gcggcctacg ggccctgctg agcggcttcc tgggcgccga catccccgcc accggcatcc
     2461 agttccagcc ccgcacggtg gtcgtgtcgc tgctggtcgg cgtcgtcgtg accgtgctgg
     2521 cggcgctgct gccggccctc aaggcgaccc gggtgccgcc ggtggccgcc ctccagccgg
     2581 agacggcgtt cgccccgacc ggcttccgca agcgccggat cgtgctcggc gtgctggtca
     2641 ccgccgtcgg cgtggccgcg ctgctggccg gcctgttccg cagcgagggc aaccagctgg
     2701 tcaacgtcgc ctccggggcc gtgatcgtct tcttcggcgt ggccatcctg agcccgctga
     2761 tcgcgcggcc cctggcccgg gtcatcggct ggccgttcgc tcgcgccttc cggctgcccg
     2821 gcaccctggc ccgccagaac gccatgcgca acccgcggcg gaccgcctcg accgcggccg
     2881 ccctgatgat cgggctggcc ctggtcgcct tcgtcagcat cttcgccgcg tcgatcaagg
     2941 cctcgaccag caagaccctg gaggacacgg tctcggccga ctacatcctg ttcaacgaca
     3001 acttccagcc gttcagcccc gaggcggccg agcgcctggc cgagcagccg gagctggcgg
     3061 cggtggccgg gatgcggatc agcccctgga agctcaacgg ggccggcaag agcctcgccg
     3121 gcatcgaccc ggccgtctac cagcaggtcg tccgcaccga gaccacggcc ggctcgctga
     3181 acgacctggc ctcgggcggc ctg
//
LOCUS       DASSWR010000526         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1205571, whole genome shotgun sequence.
ACCESSION   DASSWR010000526 DASSWR010000000
VERSION     DASSWR010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205571"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..551
                     /gene="purE"
                     /locus_tag="VFO47_08835"
     CDS             87..551
                     /gene="purE"
                     /locus_tag="VFO47_08835"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET8788840.1"
                     /translation="MPPRVGIVLGSTSDEEKAKTAAVMLEKFGVEYELEVLSAHRDPR
                     RVAEYGASAVDRGLQVIIAAAGKAAALPGVLAAHTTLPVIGLPIFTPQLGGQDSLYSI
                     VQMPSGVPVAAVGLDAAANAGILAVQILATGDPRLRLQLQEFKDQLAEGLRL"
     gene            555..1853
                     /gene="purB"
                     /locus_tag="VFO47_08840"
     CDS             555..1853
                     /gene="purB"
                     /locus_tag="VFO47_08840"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET8788841.1"
                     /translation="MIPRYTLPEMGAIWSEATRFRLFVEVELAVVRARARRGLVPATD
                     LAVIEEAPAPAPERVHQLDAQLHHDVIAFLTAYGEGIGEAARHVHYGMTSSDLLDTTL
                     ALQLTRACDLLARRLDRLVTATRDRALEHRDTLCLGRTHGVAAEPTTFGLKLAGHAFA
                     FDRDRRRLAAARDAVAVGTVSGAVGTYANLPAEIEAEVLGELGLRPEPAPTQVVARDR
                     HAELLAALAVVGADVERLATEVRHLQRTEVREAEEPFAEGQKGSSAMPHKRNPIVAER
                     LCGMARLLRGYATAGLEDVALWHERDISHSAVERVVLPDACCVLDYALERAHWLVAGL
                     RVFPERMRANLASSGGLAGSSAALLALVDAGWTREDAYTAVQRAAMAAWEGKGGFRDL
                     LLAEPGVAEVLSGPALDAALDPARFVAGAGAVFERLEALP"
     gene            1859..2761
                     /locus_tag="VFO47_08845"
     CDS             1859..2761
                     /locus_tag="VFO47_08845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET8788842.1"
                     /translation="MTAPDGLRHLGSGKVRDLYEVDDGPLLLVASDRISAFDVVLDDP
                     VPGKGEVLTALTVFWLDLLADLVPNHLAGWRASELPPSARHLAGRAMLVRRLDMVPFE
                     CVARGYLVGSGWKDYQATGSVCGIPLPGGLRQADRLPEPIFTPATKADTGHDENVSEA
                     AVAEALGPELAGRLRDLTLEVYRRGAEHAARRGILLADTKLEFGRDGDQVVLADEVLT
                     PDSSRFWPAGGWSPGHSPPSFDKQYVRDWLQDHAWDQRPPAPALPPDVVAGTAARYRE
                     AYERLAGRPLDAWLAEARDAAPVS"
     gene            2814..3155
                     /locus_tag="VFO47_08850"
     CDS             2814..3155
                     /locus_tag="VFO47_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET8788843.1"
                     /translation="MDDAVRDYIDAIPSEHRPLFDRLHRLVLEARPDAAVVLSYQIPT
                     YKVGRRRLFLGVWRHGVSVYGWQEGRDAGFSERHPELKSGKGTIRLRPADAAGISDDE
                     LRDLARAALDA"
BASE COUNT          389 a         1268 c         1144 g          407 t
ORIGIN      
        1 tcgagaacgc cgaggtggcc gaacaaggga ggctgcccta gttccgttct agaatgcccc
       61 gaccaggacc ggatcaagga gcgttcatgc cgccaagggt tgggatcgtg ctcggctcga
      121 cctccgacga ggagaaggcc aagaccgcgg cggtcatgct ggagaagttc ggcgtcgagt
      181 acgagctgga ggtgctcagc gcccaccgcg acccgcggcg ggtggccgag tacggggcca
      241 gcgccgtcga ccgcgggctc caggtgatca tcgccgccgc cggcaaggcg gccgccctgc
      301 ccggggtgct ggccgcccac accaccctgc ccgtgatcgg gctgccgatc ttcaccccgc
      361 agctcggcgg ccaggactcg ctgtactcga tcgtgcagat gccgtcgggg gtgccggtgg
      421 ccgccgtcgg gctcgacgcc gccgccaacg ccggcatcct ggccgtgcag atcctggcca
      481 cgggggaccc ccggctccgc ctccagctgc aggagttcaa ggaccagctg gccgagggcc
      541 tgcggctgta gcccatgatc ccccggtaca ccctccccga gatgggcgcc atctggagcg
      601 aggcgacccg cttccggctg ttcgtcgagg tcgagctggc cgtggtcagg gccagggccc
      661 gccgggggct ggtgcccgcg accgacctgg ccgtgatcga ggaggccccg gcccccgcgc
      721 ccgagcgggt ccaccagctc gacgcccagc tgcaccacga cgtgatcgcc ttcctgaccg
      781 cctacgggga ggggatcggc gaggccgccc ggcacgtcca ctacggcatg acctcctccg
      841 acctgctcga caccaccctg gccctgcagc tcacccgggc ctgcgacctg ctggcgcgcc
      901 ggctcgaccg cctcgtcacg gccacccggg accgcgccct tgagcaccgc gacaccctct
      961 gcctgggccg cacccacggg gtggcggccg agccgaccac cttcggcctc aagctggccg
     1021 gccacgcctt cgccttcgac cgcgaccggc gccggctggc cgccgcccgg gacgccgtcg
     1081 ccgtcggcac cgtctcgggg gcggtcggca cctacgccaa cctgcccgcc gagatcgagg
     1141 ccgaggtcct cggcgagctg ggcctgcggc ccgagccggc cccgacccag gtcgtggccc
     1201 gcgaccggca cgccgagctg ctggccgccc tggccgtggt cggggccgac gtcgagcgcc
     1261 tggccaccga ggtccgccac ctgcagcgga ccgaggtccg ggaggccgag gagccgttcg
     1321 ccgagggcca gaagggctcc tcggccatgc cccacaagcg caacccgatc gtggccgagc
     1381 gcctctgcgg catggcccgg ctgctgcgcg gctacgccac cgccggcctg gaggacgtcg
     1441 ccctctggca cgagcgcgac atctcccact cggccgtgga gcgggtcgtc ctgcccgacg
     1501 cctgctgcgt gctcgactac gccctggagc gggcccactg gctggtggcc ggcctgcggg
     1561 tcttccctga gcggatgcgg gctaatctag cttcctcggg cgggctcgcc gggtcctcgg
     1621 ccgccctgct ggccctggtc gacgccggct ggacgcggga ggacgcctac accgcggtgc
     1681 agcgggcggc catggccgcc tgggagggca agggcggctt ccgcgacctg ctcctggccg
     1741 agccgggcgt ggccgaggtg ctgtccgggc ccgccctgga cgccgccctc gacccggccc
     1801 ggttcgtggc cggggccggc gccgtgttcg agcgactgga ggcgctgccg tgagcgagct
     1861 gacggccccg gacgggctgc ggcacctggg gtccggcaag gtccgtgacc tgtacgaggt
     1921 cgacgacggc cccctgctgc tggtggccag cgaccgcatc tccgccttcg acgtggtcct
     1981 ggacgacccc gtccccggca agggcgaggt cctgaccgcc ttgaccgtgt tctggctcga
     2041 cctgctcgcc gacctcgtcc ccaaccacct ggccggctgg cgggcctcgg agctgccccc
     2101 gtccgcccgg cacctggccg gccgggccat gctggtccgg cgcctggaca tggtgccgtt
     2161 cgagtgcgtg gcccgcggct acctggtcgg gtcgggctgg aaggactacc aggccaccgg
     2221 gtcggtctgc ggcatccccc tgcccggcgg gctgcgccag gccgaccggc tgcccgagcc
     2281 catcttcacc ccggccacca aggccgacac cggccacgac gagaacgtct cggaggcggc
     2341 ggtggccgag gccctcgggc ccgagctggc cgggcggctg cgcgacctga ccctcgaggt
     2401 ctaccggcgg ggggccgagc acgccgcccg gcgcggcatc ctgctggccg acaccaagct
     2461 cgagttcggc cgcgacggcg accaggtggt cctggccgac gaggtcctca cccccgactc
     2521 gtcccggttc tggccggccg gcggctggtc cccggggcac tccccgccct cgttcgacaa
     2581 gcagtacgtg cgcgactggc tccaggacca cgcctgggac cagcgccccc cggccccggc
     2641 gctgcccccc gacgtggtcg ccggcacggc cgcccgctac cgcgaggcct acgagcgcct
     2701 cgccggccgc cccctcgacg cctggctggc cgaggccagg gacgcggcgc cggtcagctg
     2761 acccttgccc acctccgggt cgagccaggc ccgccggagc gatactgggg cccatggacg
     2821 acgccgtgcg ggactacatc gacgccatcc cctccgagca ccgccccctg ttcgaccggc
     2881 tccaccggct ggtcctggag gcccgccccg acgccgcggt ggtgctctcc taccagatcc
     2941 cgacctacaa ggtcggccgc cgccgcctct tcctcggcgt gtggaggcac ggggtgtcgg
     3001 tgtacggctg gcaggagggc cgcgacgccg gcttcagcga gcgccacccc gagctcaagt
     3061 ccggcaaggg gaccatccgg ctgcgtcccg ccgacgccgc cggcatctcc gacgacgagc
     3121 tccgcgacct ggcccgggcc gccctggacg cctgaccgcg gggcgcggcg gctagggaac
     3181 cagccggtag aaccgccaga cgtcggcg
//
LOCUS       DASSWR010000527         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_274192,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000527 DASSWR010000000
VERSION     DASSWR010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274192"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1320)
                     /locus_tag="VFO47_08855"
     CDS             complement(19..1320)
                     /locus_tag="VFO47_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HET8788844.1"
                     /translation="MATERHDVLVIGGGPAGAAAAFWLAKAGHDVCVLERKAFPRDKT
                     CGDGLTPRAVHQLRAMGLEPAIAAHHHRHEGLRAVAHGITLELRWPEHPVFPDYGYVV
                     RRRDLDRLVADHAAAAGATVLQATEAKRPLLADGLVTGAVVEDKTTGETREVRARYVI
                     VADGSLSRFGRALGAARNRSYPQGIAIRGYFESPISDDPWIESCLDVHDRAGRSLPGY
                     GWIFPLGDGTINVGIGLLSTFKGYKDVNTTHLMQEWAATAPARWGIDPDAVLAPPTGG
                     RLPMAGSVHPKAGPNWLLVGDAAGSINPFNGEGIDYAYETARLAADLVDEALRTGSAA
                     PLARYQARLDEEYGLYFKVARHFATIIGRPALMRELTRVGMRSRSLMEWVLRIMANLL
                     REDELGPAEAAYRAVARLARLVPEPVRSASSTRSAARRAVS"
     gene            complement(1366..2118)
                     /locus_tag="VFO47_08860"
     CDS             complement(1366..2118)
                     /locus_tag="VFO47_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8788845.1"
                     /translation="MTEIPEHLLKRSRERRAALGLGGDAPASAPAEAGEAEEAPAPAA
                     AAPTPAPAAPAEVEKPTPPPPEPVYIQAAKRRKRIPYWAASVLVALPLWGYMYVRTLE
                     PPPAGETDPVAMGAEIYSGNCASCHGASGQGASGPALADGAVVETFPDWRDHAAWVRL
                     GTDGWPAATYGATEKPVGGGGLMPAWPTLTDQELAQVVLYERQLSGEDVSEANEDYAD
                     LRAVATGEMTLAEAGLGELSEAAGVTEADLGG"
     gene            2405..>2713
                     /locus_tag="VFO47_08865"
     CDS             2405..>2713
                     /locus_tag="VFO47_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788846.1"
                     /translation="MVAFICTLLVGFGGVAGVLLYGRRRPVGAPLSWGEAMAAAVFAM
                     FLLLWWYGVIPHQWLTWSEGELGWRSDRLLLEPTANQPLTVSYKTLADIIAVVIYGVG
                     I"
BASE COUNT          317 a          987 c         1048 g          361 t
ORIGIN      
        1 cgagggcatc gactacgcct acgagaccgc ccgcctggcc gccgacctgg tcgacgaggc
       61 gctgcggacc ggctcgggca ccaggcgggc cagccgggcg acggcccggt aggcggcctc
      121 ggccgggccg agctcgtcct cgcgcagcag gttggccatg atccgcagca cccactccat
      181 gagcgaccgc gaccgcatgc ccacccgggt gagctcgcgc atcagcgccg gccggccgat
      241 gatcgtggcg aagtgccggg cgaccttgaa gtagaggccg tactcctcgt cgaggcgcgc
      301 ctggtaccgg gcgagcggcg ccgccgagcc ggtccgcagc gcctcgtcga ccaggtcggc
      361 ggccaggcgg gcggtctcgt aggcgtagtc gatgccctcg ccgttgaagg ggttgatcga
      421 gccggcggcg tcgcccacga gcagccagtt gggcccggcc ttggggtgga ccgacccggc
      481 catcggcagg cggcccccgg tcggcggggc gagcacggcg tcggggtcga tgccccagcg
      541 ggcgggggcg gtggccgccc actcctgcat caggtgggtg gtgttgacgt ccttgtagcc
      601 cttgaacgtc gacagcaggc cgatgccgac gttgatggtc ccgtcgccca gcgggaagat
      661 ccagccgtag ccgggcagcg accgacccgc ccggtcgtgg acgtcgaggc acgactcgat
      721 ccaggggtcg tccgagatcg gcgactcgaa gtagccccgg atggcgatgc cctgggggta
      781 ggacctgttg cgggccgcgc cgagcgcccg cccgaagcgg gacagcgagc cgtcggccac
      841 gatgacgtag cgggcccgga cctcccgggt ctcgcccgtc gtcttgtcct cgacgaccgc
      901 acccgtgacc agcccgtcgg ccagcagcgg ccgcttggcc tcggtggcct gcaggacggt
      961 ggcgccggcg gcggccgcgt ggtcggccac cagccggtcg aggtcgcgcc ggcggacgac
     1021 gtagccgtag tcggggaaga cggggtgctc gggccagcgg agctcgaggg tgatgccgtg
     1081 ggcgacggcc cggaggccct cgtggcggtg gtggtgcgcg gcgatcgccg gctcgagccc
     1141 catggcgcgc agctggtgga ccgcccgggg ggtgagcccg tcgccgcagg tcttgtcgcg
     1201 ggggaaggcc ttgcgctcga gcacgcagac gtcgtggccg gccttggcca gccagaaggc
     1261 ggcggcagcc cccgcggggc cgccgccgat gacgaggacg tcgtgccgct ccgtggccat
     1321 ggggcccgag cctagggcgc cggcgggccg gcgccgcagg ccgggtcagc cgccgaggtc
     1381 ggcctcggtg acgccggcgg cctccgacag ctcgccgagg cccgcctcgg ccagggtcat
     1441 ctcgcccgtg gcgacggcgc gcaggtcggc gtagtcctcg ttggcctcgg acacgtcctc
     1501 gcccgagagc tgccgctcgt agaggacgac ctgggcgagc tcctggtcgg tgagcgtggg
     1561 ccaggccggc atcaggccgc ccccgcccac cggcttctcg gtggcgccgt aggtggcggc
     1621 aggccagccg tcggtgccga gccgcaccca ggcggcgtgg tcgcgccagt cggggaaggt
     1681 ctcgaccaca gcgccgtccg cgagggccgg gccggaggct ccctggccgc tggcgccgtg
     1741 acaggaggcg cagttgccgc tgtagatctc ggcgcccatg gcgaccgggt cggtctcgcc
     1801 tgcgggcggc ggctcgagcg tgcggacgta catgtagccc cagagcggga gcgcgacgag
     1861 caccgacgcc gcccagtagg ggatgcgctt gcggcgcttc gccgcctgga tgtacacggg
     1921 ctcgggcggc gggggcgtcg gcttctcgac ctcggccggt gcggccggcg cgggcgtcgg
     1981 tgcggccgcc gcgggggccg gcgcctcctc ggcctcgccg gcctcggccg gcgcgctcgc
     2041 gggtgcgtcg ccgcccaggc cgagggcggc acggcgctcg cgggagcgct tcagcaggtg
     2101 ctcggggatt tcggtcaacg cagcctccgc ggtcggactg agtgcaggtg gcccctcgcc
     2161 cgcccagcgc acgactccgg ctgccggaag gcggtcgacg ggtccccgtc ctcactcgac
     2221 gggccacttt atgcacctcc ccgcggccgc caccaagccc ggccaccagg tcggccgggg
     2281 ccccacgggg cccggggggc ggatgtgaac ctcccggaac ccccgtgcta aacctgccca
     2341 cgtcgccggt cacagccggc cggcgacctc caatgtgagc gatttcacga ggagtcgctg
     2401 gtccttggtc gccttcatct gcactctcct ggtcggcttc ggcggcgtcg ccggggtcct
     2461 cctgtacggc aggcgccgcc cggtcggcgc gccgctctcg tggggcgagg cgatggcggc
     2521 cgcggtgttc gccatgttcc tcctgctctg gtggtacggc gtcatccccc accagtggct
     2581 cacctggtcg gagggcgagc tcggctggcg gtccgatcgc ctcctgctcg agcccaccgc
     2641 caaccagccg ctcacggtca gctacaagac gctcgccgac atcatcgccg tcgtcatcta
     2701 cggcgtcggg atc
//
LOCUS       DASSWR010000528         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_895730,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000528 DASSWR010000000
VERSION     DASSWR010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895730"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..227)
                     /locus_tag="VFO47_08870"
     CDS             complement(48..227)
                     /locus_tag="VFO47_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788847.1"
                     /translation="MAVLAEARVRAGERLRVEASGPGRIVLTRAEDSFEDLLGAVGDD
                     VYPPGYLQDLRGEWD"
     gene            complement(274..1398)
                     /locus_tag="VFO47_08875"
     CDS             complement(274..1398)
                     /locus_tag="VFO47_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET8788848.1"
                     /translation="MADATGKGLEGVVAAQTRISDVDGQQGRLWYAGYDIADLTAHAS
                     FEETIHLLHHLELPTAAQLSETVAMLRAESTLDPLLVDLVGSLAERTGPMTTLRTAVS
                     AAAALDPDDEEPEEANLRKAYRLMAKTPQVIATYQRLRTGGDVIEPRDDLDIAANFLY
                     ALTGKQPDPQVARDFDVVLVLYADHTLNASTFAGRVAAATMADMYAAVTAAVATLQGP
                     LHGGAIEEVREMLEDIGQPDRAPDWVRERLAAKQKVMGFGHRVYKTWDPRATLLREIA
                     ERLGKQFGETRWYDTSVRVQEAVMEAKPLYPNVDFYTASVYSALGLPGDLYTPLFAAA
                     RMAGWTAHIREQYADNRLIRPDSEYIGPNPRAWKPLESRG"
     gene            1517..2644
                     /locus_tag="VFO47_08880"
     CDS             1517..2644
                     /locus_tag="VFO47_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET8788849.1"
                     /translation="MAHRVTFIPGDGVGPELSEATRRVLEGTGVGFDWDVQEAGAEVI
                     DKYGTPLPDQVLDSIRSNGVAIKGPLTTPIGTGFRSVNVALRKELDLFACLRPCKTYQ
                     GVRSRYDDIDLVVVRENHEDLYAGIEFEQGTPDAAKVIEFLNGIQPKKIRDDSGVSIK
                     PMSITGTRRIAKFAFDYARAYGRKKVTAVTKANIMKHTDGLFYAVAREVARDYDDVEY
                     DEYLVDNMCMQLVQKPENFDVLLLPNLYGDIISDLGAGLVGGLGVAPGANLGDRVAVF
                     EATHGSAPKYKGQNKVNPMAMMLSGMLLLRHLEEGEAADRLERALAAVIAEGKSVTYD
                     MKPSRDDPTAVGTSQVADAVIAKMDEGSGELKVSSPDGGSA"
     gene            2641..3579
                     /gene="mdh"
                     /locus_tag="VFO47_08885"
     CDS             2641..3579
                     /gene="mdh"
                     /locus_tag="VFO47_08885"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET8788850.1"
                     /translation="MMPKVTVVGAGKYGSTTVQRLAEKDICDEVVMTDIVEGLPQGLA
                     LDMNQSRPIEGFETRVVGSNGYEETAGSDVVVITAGVPRKPGMSRMDLLETNAKIVGE
                     VTRKVADGSPDAVLMVVSNPLDEMTALAAEVSGFPRERVMGQAGMLDTARFKHFLAEE
                     LGTSPSRVEAMTLGSHGDTMVPVPSMVKVDGKPLTEVADAETIERLVQRTRDGGAEVV
                     ALLKSGSAYYAPSSAAAAMVAAVLGDTGEVMPVCAWVTGQYGIDGVYLGVPAKLGRAG
                     VAEVVELPLTDEELADLREAAEAVRSKQADVAKLVG"
BASE COUNT          539 a         1247 c         1347 g          519 t
ORIGIN      
        1 ccacgacctg gacccgctcg atgcccttcc acctggcgtc cgcagcgcta gtcccattcg
       61 ccgcggaggt cttgcagata gcccggcggg tagacgtcgt ccccgacggc gccgagcagg
      121 tcctcgaagc tgtcctcggc ccgggtgagc acgatccgcc ccggtcccga cgcctcgacc
      181 cgcagccgct ccccggcccg caccctcgcc tcggcgagca cggccaccgg gatggtcgcc
      241 tgatgcttgc ggctcagccg ggtcgtccgc ggcctacccc cgggactcca ggggcttcca
      301 ggcgcggggg ttgggaccga tgtactccga gtccgggcgg atcaggcggt tgtcggcgta
      361 ctgctcgcgg atgtgggccg tccagccggc catgcgggcg gcggcgaaca ggggggtgta
      421 gaggtcgccg gggaggccga gggcggagta gacgctggcc gtgtagaagt cgacgttggg
      481 gtagaggggc ttggcctcca tgaccgcctc ctggacccgg accgaggtgt cgtaccagcg
      541 ggtctcgccg aactgcttgc cgaggcgctc ggcgatctcg cgcaggaggg tggcccgggg
      601 atcccaggtc ttgtagacgc ggtggccgaa gcccatcacc ttctgcttgg cggcgaggcg
      661 ctcgcgaacc cagtcggggg cccggtcggg ctggccgatg tcctccagca tctcgcggac
      721 ctcctcgatg gccccgccgt ggagcgggcc ctggagggtg gccacggccg cggtgaccgc
      781 ggcgtacatg tcggccatgg tggcggcggc gacccggccg gcgaaggtgg aggcgttcag
      841 ggtgtggtcg gcatacagca ccaggacgac gtcgaagtcg cgggcgacct gcgggtcggg
      901 ctgcttgccg gtgagggcgt agaggaagtt ggcggcgatg tcgaggtcgt cgcgcggctc
      961 gatcacgtcc ccgcccgtcc gcagccgctg gtaggtggcg atgacctgcg gggtcttggc
     1021 catcagccgg taggccttgc ggaggttggc ctcctccggc tcctcgtcgt cggggtcgag
     1081 ggcggcggcg gccgacaccg ccgtccgcag ggtggtcatg gggccggtcc gctcggccag
     1141 ggagccgacc aggtcgacca gcagcgggtc aagggtgctc tcggcccgca gcatggccac
     1201 cgtctcggac agctgggcgg ccgtcggcag ctccaggtgg tgcagcaggt ggatggtctc
     1261 ctcgaagctg gcgtgggcgg tcaggtcggc gatgtcgtag ccggcgtacc acagcctgcc
     1321 ctgctggccg tcgacgtcgg agatcctggt ctgggcggcg accacgccct cgagtccctt
     1381 gcccgttgcg tcagccatct cggggtgcct ccggtcgtct cggggggcgg gtccaccgca
     1441 atcgtagggg ttttcctggc gcatccgccg tacagagata cccttggcga cacctcgagc
     1501 aaaggagcgc cccaccatgg cccatcgcgt cacgttcatc cccggggacg gggtaggacc
     1561 ggagctgtcg gaggcgaccc gccgggtcct cgagggcacc ggcgtggggt tcgactggga
     1621 cgtccaggag gccggggccg aggtcatcga caagtacggg acccccctgc ccgaccaggt
     1681 gctcgactcc atccggagca acggggtggc gatcaagggg ccgctgacca ccccgatcgg
     1741 caccgggttc cgcagcgtca acgtggccct gcgcaaggag ctcgacctgt tcgcctgcct
     1801 gcggccctgc aagacctacc agggggtccg gtcccgctac gacgacatcg acctggtggt
     1861 ggtgcgcgag aaccacgagg acctgtacgc cggcatcgag ttcgagcagg gcacgcccga
     1921 cgcggccaag gtcatcgagt tcctcaacgg catccagccc aagaagatcc gggacgactc
     1981 cggcgtctcg atcaagccga tgtcgatcac cgggaccagg cggatcgcca agttcgcctt
     2041 cgactacgcc cgcgcctacg ggcgcaagaa ggtcacggcg gtcaccaagg cgaacatcat
     2101 gaagcacacc gacgggctct tctacgccgt ggcccgcgag gtggccaggg actacgacga
     2161 cgtcgagtat gacgagtacc tggtcgacaa catgtgcatg cagctggtgc agaagccgga
     2221 gaacttcgac gtgctgctgc tgcccaacct gtacggcgac atcatctccg acctcggggc
     2281 cgggctcgtc ggcgggctcg gggtcgcgcc cggggccaac ctgggggatc gggtggcggt
     2341 gttcgaggcc acccacggct cggctcccaa gtacaagggc cagaacaagg tcaacccgat
     2401 ggcgatgatg ctctcgggca tgctgctgct gcgccacctg gaggagggcg aggccgccga
     2461 ccggctggag cgggccctgg ccgcggtcat cgccgagggc aagagcgtca cctacgacat
     2521 gaagccgtcg cgcgacgatc ccacggccgt cggcaccagc caggtcgccg acgcggtcat
     2581 cgccaagatg gacgaggggt ccggggagct caaggttagc tccccggacg gagggagcgc
     2641 ctgatgccga aggtcaccgt ggtcggggcg ggcaagtacg gctccaccac cgtgcagcgc
     2701 ctggccgaga aggacatctg cgacgaggtc gtgatgaccg acatcgtcga gggcctcccc
     2761 cagggcctgg ccctcgacat gaaccagtcg cggcccatcg aggggttcga gacccgggtg
     2821 gtcggcagca acggctacga ggagacggcc ggctcggacg tggtcgtgat caccgccggg
     2881 gtgccacgca agcccggcat gagccgcatg gacctgctgg agaccaacgc caagatcgtc
     2941 ggcgaggtca cccgcaaggt cgccgacggc tcgccggacg cggtcctgat ggtggtctcc
     3001 aacccgctgg acgagatgac ggccctggcc gccgaggtgt ccgggtttcc cagggagcgg
     3061 gtcatgggcc aggccgggat gctcgacacg gcccggttca agcacttcct ggccgaggag
     3121 ctgggcacca gcccgagccg ggtcgaggcc atgaccctcg gctcccacgg cgacaccatg
     3181 gtcccggtgc cgagcatggt caaggtcgac ggcaagccgc tcaccgaggt cgccgacgcc
     3241 gagacgatcg agcgcctggt ccagcggacc cgcgacggcg gggccgaggt ggtcgccctg
     3301 ctcaagtcgg gctcggccta ctacgcgccc tccagcgcgg ccgcggccat ggtggccgcc
     3361 gttctcggcg acaccggcga ggtcatgccc gtctgcgcct gggtcaccgg ccagtacggc
     3421 atcgacggcg tctacctggg cgtcccggcc aagctgggcc gcgccggggt cgccgaggtg
     3481 gtcgagctcc ccctgaccga cgaggagctg gccgacctcc gcgaggccgc cgaggccgtc
     3541 cgctccaagc aggccgacgt ggccaagctg gttggctgac caggcgggcc tgggcccggc
     3601 cgccggagcc gccccgggcc cggccgtcgg ggccgcttcc ggctgtgccc tg
//
LOCUS       DASSWR010000529         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1347903, whole genome shotgun sequence.
ACCESSION   DASSWR010000529 DASSWR010000000
VERSION     DASSWR010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347903"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFO47_08890"
     CDS             <1..876
                     /locus_tag="VFO47_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8788851.1"
                     /translation="EEAGVDTVWVPDHLLQADPTAGPGDTEMLEAYTTLGFLAAQTDR
                     VRLGTMVTGVTFRPPALLVKAVTTVDVLSGGRAWLGIGAGYHGEEADAMGLPLPPVAE
                     RFQRLEETLQIAARMWAGDDTPFAGRHYRLGRPVGSPPPTQRPGPPILVGGAGERKTL
                     RLVARYADACNLFDIPDGGRTVTHKLAVLARHCADLGRPYEEIEKTLSTRLEAGEPAD
                     AFVARCREAAALGIEHVVVLTPGPWTRDRLAVLAEAIPVVGELPVARAAATRRKVRAW
                     TEGRGGGGESTFPRG"
     gene            894..2048
                     /locus_tag="VFO47_08895"
     CDS             894..2048
                     /locus_tag="VFO47_08895"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8788852.1"
                     /translation="MALAVAITVAISVGADQGAPPDAFAYGLGLTIAALALLRRRWPL
                     PVLLASAAILVFYNQFDYPGLFAAVPLSVALATAWAAGYRVWALAVVLWFGGSPLVWF
                     ALYAPQALPEESATRLLNSAVSDLALLAAVLLLGEAVRTRRALDREHRLLVAEQDRSE
                     RLLLNVLPAPVADRLKQDEAVIADRFPEVTVLFADLVDFTRSSDQSSPERVVQVLNDL
                     FTAFDRLARRHGLEKIKTIGDAYMVAGGLPEPRPDHAQAVAQMALAMREEASRHADPA
                     GQPLQVRIGIDTGPVVAGVIGTTKFSYDLWGDTVNTASRMESHGVEGCIQVTERTYRR
                     LKDRYRFQRRGPIQVKGKGTQDTWFLVARAVSPAEPRGASPPRTRSRTGR"
     gene            complement(1987..>2211)
                     /locus_tag="VFO47_08900"
     CDS             complement(1987..>2211)
                     /locus_tag="VFO47_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788853.1"
                     /translation="DGHEIGGIMGVPGVSSGWTTLFEVADTDAAVARAAEAGGTAGTP
                     EDFVYGRLATITDPFGTEFSVGSRPEAQRD"
BASE COUNT          282 a          824 c          804 g          301 t
ORIGIN      
        1 gaggaggccg gggtcgacac cgtctgggtg cccgaccacc tgctccaggc cgacccgacc
       61 gccggtcccg gcgacaccga gatgctggag gcctacacca ccctggggtt cctggccgcc
      121 cagaccgatc gggtccggct cggcaccatg gtcaccggcg tgaccttccg gcccccggcc
      181 ctgctggtca aggccgtcac caccgtggac gtgctctcgg gcgggcgcgc ctggctgggg
      241 atcggcgccg gctaccacgg cgaggaggcc gatgccatgg gcctgcccct gcccccggtg
      301 gccgagcggt tccagcggct ggaggagacg ctccagatcg cggcccggat gtgggccggc
      361 gacgacaccc cgttcgccgg ccgccactac cggctggggc ggccggtggg cagcccgccg
      421 cccacccagc gtcccgggcc accgatcctg gtcggaggcg ccggcgagcg caagaccctg
      481 cggctggtcg cccggtatgc cgacgcctgc aacctgttcg acatccccga cggcggccgc
      541 accgtcaccc acaagctcgc ggtcctggcc aggcactgcg ccgacctcgg ccgcccctac
      601 gaggagatcg agaagaccct cagcacccgg ctggaggccg gggagcccgc cgacgcgttc
      661 gtcgcccgtt gccgggaggc ggccgcgctc ggcatcgagc acgtggtcgt cctcaccccg
      721 ggtccgtgga cccgggaccg gctggccgtg ctcgccgaag ccatccctgt cgtcggggag
      781 cttccggtcg cccgggcggc agcgacccgg cgtaaggtcc gggcatggac ggaaggtcgg
      841 ggcggtggag gcgagagcac cttcccgcgt ggctgatcga tgccggattg tcggtggccc
      901 tggcggtcgc gatcacggtc gcgatcagcg tcggggcgga ccagggcgct ccgcccgacg
      961 cgttcgccta cgggctcggg ctgaccatcg ccgccctggc gctgctccgg cgacggtggc
     1021 ccctgccggt gctgctcgcg tccgcggcga tcctcgtgtt ctacaaccag ttcgactatc
     1081 cggggctctt cgccgccgtg ccgctctcgg tcgcgctggc caccgcctgg gcggccggtt
     1141 accgcgtctg ggcgctggcg gtcgtcctgt ggttcggtgg ttcaccactc gtgtggttcg
     1201 ccttgtacgc cccgcaggcg ctgcccgagg agtcggccac ccggctgctc aacagcgccg
     1261 tctccgacct cgcgctgctg gccgccgtgc tgctgctcgg ggaggcggtg cgcacccggc
     1321 gcgccctgga ccgggagcac cgcctgctgg tggccgagca ggaccgctcc gagcggctcc
     1381 tgctcaacgt cctcccggca ccggtcgccg accggctcaa gcaggacgag gccgtgatcg
     1441 ccgaccggtt cccggaggtc acggtgctct tcgccgacct ggtcgacttc acccgcagca
     1501 gcgaccagag cagcccggag cgggtggtgc aggtcctgaa cgacctgttc accgccttcg
     1561 accggctggc ccggcgccac gggctggaga agatcaagac catcggggac gcctacatgg
     1621 tggccggcgg gctgcccgag ccccgccccg accatgccca ggcggtggcg cagatggccc
     1681 tggccatgcg ggaggaggcc agccggcatg ccgacccggc gggccagccg ctccaggtcc
     1741 gcatcggcat cgacaccggc ccggtggtgg ccggggtgat cggcaccacc aagttcagct
     1801 acgacctgtg gggcgacacc gtgaacaccg ccagccgcat ggagtcgcac ggggtcgagg
     1861 gctgcatcca ggtgaccgag cggacctacc ggcggctcaa ggaccgctat cggttccagc
     1921 ggcggggacc gatccaggtc aagggcaagg ggacacagga cacttggttc ctggtcgccc
     1981 gggcggtcag tcccgctgag cctcggggcg cgagcccacc gagaactcgg tcccgaacgg
     2041 gtcggtgatg gtggccaggc gcccgtagac gaagtcctcg ggtgtgccgg cggtgccgcc
     2101 ggcctcggcg gcgcgggcga ccgcggcgtc ggtgtcggcc acctcgaaca gggtcgtcca
     2161 gcccgacgac acgccgggga cgcccatgat cccgccgatc tcgtgaccgt c
//
LOCUS       DASSWR010000530         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_217608,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000530 DASSWR010000000
VERSION     DASSWR010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217608"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFO47_08905"
     CDS             complement(<1..345)
                     /locus_tag="VFO47_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788854.1"
                     /translation="MTNQTAEAFEEVAPGDIEQALERQRVQGENGGGAVVRASVANLV
                     VVTPNRAAGQQALAAVEELGARAPSRCVVLIAEPPEGRQGVRSWARVVNRRPAQGPEV
                     VWEEVVVQTNVHP"
     gene            complement(342..1880)
                     /gene="zwf"
                     /locus_tag="VFO47_08910"
     CDS             complement(342..1880)
                     /gene="zwf"
                     /locus_tag="VFO47_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET8788855.1"
                     /translation="MEENPLAVGERVRAIPPPTAMVVFGGSGDLAHRKIVPALYNLEL
                     HRLLPQNFAFVGSSRSEYSDEEYRADMRKAVEEFSRTQPIQHQVWESFASRLHYVSGS
                     SADPDTYARIGDLLDRFDRELGTDGNRMYYLSLIPKLFPDTIRGIGKAGLGGRGWDRG
                     HSRVVVEKPFGHDLTSARELQELVTTVFPERDVFRIDHYLGKESVQNIIAFRFANGIF
                     EPVWNHHFVDHVQVTVSESLGIEHRGNFYETAGAIRDIFQNHELQVLALIAMEPPASF
                     EPEDVRNEKAKVLKALRRLEGEAVDAGVVRAQYGPGWIAGKEVPGYRDEPDVHPDSTV
                     ETYVAAELRVDNWRWAGTPFYVRTGKRLPKRATEVVVQFKSAPHLPFGEAAQDGLEPN
                     ALVLRIQPDEGITMRIGAKVPGPSMEVRSVSMDFSYGTSFSDDLPDAYERLLLDVMLG
                     DPTLFPRWDEVEQAWQAVQPILDRWAEAPPPQFPNYEAGSWGPKAADELIARGRPGRR
                     WRRP"
     gene            complement(1880..>2571)
                     /locus_tag="VFO47_08915"
     CDS             complement(1880..>2571)
                     /locus_tag="VFO47_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018817340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET8788856.1"
                     /translation="DPAGPLPHRLHVGGGGADVVKGQRLEDPPRVQALGGLEELAASG
                     GDLTEVASVASFFVSRVDTMVDPELQAIVDKGGPEAEAARERLGTAAIDNAKLAYEVF
                     QQTFRGPRWEALAAAGARVQRPLWASTSTKNPDYRDVLYVEELIAADTVNTMPLETIE
                     AFADHGEVRGQTASEGLDRAHRLWSDLAALGIDEDDVGDRLEREGVEKFADSYNGAVD
                     TIEAKVRKLGG"
BASE COUNT          324 a          925 c          937 g          385 t
ORIGIN      
        1 ggggtggacg ttggtctgga ccacgacctc ctcccagacg acctccggcc cctgggccgg
       61 gcggcggttc accacccggg cccaggagcg caccccctgg cggccctcgg gcggctcggc
      121 gatcaggacc acgcagcgcg acggcgcccg ggcccccagc tcctccaccg cggccagggc
      181 ctgctggccg gcggcccggt tgggggtgac cacgaccagg ttggccacgc tggcccggac
      241 caccgccccg ccgccgttct ccccctggac gcgctggcgc tccagcgcct gctcgatgtc
      301 gccgggcgcg acctcctcga acgcctcggc cgtctggttc gtcatggccg ccgccagcgg
      361 cggccggggc ggccccgggc gatcagctcg tcggcggcct tgggccccca gctgccggcc
      421 tcgtagttgg ggaactgcgg gggcggcgcc tcggcccagc ggtccaggat cggctggacc
      481 gcctgccagg cctgctcgac ctcgtcccag cgcgggaaca gggtcgggtc gcccagcatc
      541 acgtccagca gcagccgctc gtaggcgtcg ggcaggtcgt cggagaacga ggtgccgtag
      601 gagaagtcca tgctgaccga gcggacctcc atggacgggc cggggacctt ggccccgatc
      661 cgcatggtga tgccctcgtc gggctggatc cgcaggacca gcgcgttggg ctccagcccg
      721 tcctgggcgg cctccccgaa gggcaggtgc ggggccgact tgaactggac cacgacctcg
      781 gtggcccgct tgggcaggcg cttgccggtg cggacgtaga acggcgtgcc cgcccagcgc
      841 cagttgtcga cccgcagctc ggcggccacg tacgtctcga ccgtcgagtc gggatggacg
      901 tcgggctcgt cgcggtagcc ggggacctcc ttgccggcga tccagcccgg cccgtactgg
      961 gcgcggacca cgccggcgtc gaccgcctcg ccctccagcc ggcgcagcgc cttgagcacc
     1021 ttggccttct cgttgcgcac gtcctcgggc tcgaagctgg ccggcggctc catggcgatc
     1081 agggccagca cctggagctc gtggttctgg aagatgtcgc ggatggcccc ggcggtctcg
     1141 tagaagttgc cgcggtgctc gatccccagc gactccgaca ccgtcacctg gacgtggtcg
     1201 acgaagtggt ggttccagac cggctcgaag atgccgttgg cgaagcggaa ggcgatgatg
     1261 ttctggacgc tctccttgcc caggtagtgg tcgatgcgga agacgtcgcg ctcggggaag
     1321 acggtggtca ccagctcctg cagctcccgg gccgaggtca ggtcgtggcc gaacggcttc
     1381 tccaccacga cccgggagtg gccccggtcc cagccgcgcc cgcccagccc ggccttgccg
     1441 atcccgcgga tggtgtcggg gaacagcttg gggatcagcg acaggtagta catgcggttg
     1501 ccgtcggtgc cgagctcgcg gtcgaagcgg tcgagcaggt cgccgatgcg ggcgtaggtg
     1561 tcggggtcgg ccgaggagcc ggagacgtag tgcagccggc tggcgaacga ctcccacacc
     1621 tggtgctgga tgggctgggt gcgggagaac tcctcgaccg ccttgcgcat gtcggcccgg
     1681 tactcctcgt cggagtactc gctgcggctg gacccgacga aggcgaagtt ctgcggcagc
     1741 agccggtgca gctccaggtt gtacagggcg gggacgatct tgcggtgggc caggtcgccg
     1801 gagccgccga acaccaccat ggccgtcggc ggcgggatcg ccctgactcg ctccccaacc
     1861 gcgagtgggt tctcctccat cagccgccca gcttccgcac cttggcctcg atggtgtcga
     1921 cggcgccgtt gtagctgtcg gcgaacttct cgaccccctc gcgctccagc cggtcgccga
     1981 cgtcgtcctc gtcgatgccg agggcggcca ggtcgctcca gagccggtgc gcgcggtcca
     2041 ggccctcgct ggccgtctgg ccgcggacct cgccgtggtc ggcgaaggcc tcgatcgtct
     2101 ccaggggcat ggtgttgacc gtgtcggcgg cgatcagctc ctccacgtac aggacgtcgc
     2161 ggtagtcggg gttcttggtc gaggtcgagg cccacagggg ccgctggacc cgggccccgg
     2221 cggcggccag ggcctcccag cgcggccccc ggaaggtctg ctggaacacc tcgtaggcca
     2281 gcttggcgtt gtcgatggcg gccgtgccca gccgctcccg ggccgcctcg gcctccgggc
     2341 cgcccttgtc gacgatcgcc tgcagctccg gatcgaccat cgtgtcgacc cggctgacga
     2401 agaacgaggc cacgctggcc acctcggtca ggtcgccgcc ggaggcggcc agctcctcca
     2461 ggccgcccag ggcctggacg cgcggcggat cttcgagtcg ctggcccttg acgacatccg
     2521 cgccgccgcc gccgacatgg agccggtgtg ggagcggacc ggcggggtcg a
//
LOCUS       DASSWR010000531         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_274368,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000531 DASSWR010000000
VERSION     DASSWR010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274368"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2161
                     /gene="ligD"
                     /locus_tag="VFO47_08920"
     CDS             <1..2161
                     /gene="ligD"
                     /locus_tag="VFO47_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET8788857.1"
                     /translation="YEDVRTVAKLIKVVLDGLGLRGYAKTSGATGMQVFVPLDGTHTH
                     SDAREVVERVGRLVVRAYPEKATMAWPVADRAGKVFIDHQMNRSGANIASVYSLRPLP
                     GGPVSTPLDWDELDTDIEPGDFRIDNVWERFAAGDRFAAVLDDKQSLTPAMEALGLTP
                     GAPEPPRDRSRPRFPPRRTTRPASRASLDEYVAKRDFSRTPEPPTSVDGPPNGRVARS
                     GDEGERLSTAPTDPTAPTPTDPAPTGPMTSTGLPEEPHPAGLTEVAALEPGRRFTIQQ
                     HHARRLHHDVRFEQDEVLVSFAVPKRLPEEPGLRHLAVNTEDHPLDYLTFSGTIPAGE
                     YGGGEVRLFDLGTYEPLEVADGKLTVRLHGSRYKGAEYHLFRTRDRDWLVILKGKVSL
                     PQPAPPPTYEPMMAVLTAEAFDDDDWLFEVKWDGHRCLANLGTSTRLTSRTTRDMTAQ
                     FPELIDMHRQLAARNAVVDGEIVALDRNGRPSFERMQDRFHRTPDELARNRGRVPVQF
                     LAFDLLWLDGQSLLDLPLAERRTRLVEVLVETRDIRLSQVVEGAGKAFFDQVKALQLE
                     GIVAKRAASPYRPGTRSHDWRKIKALCLQDCVIVGWTPGKGGRAATLGSLLLAVYDGG
                     RLRYAGNVGTGFTHAFLADLLARLQELRVDKPQFEGFEGTLRPRGARFVRPELVCEVE
                     YLKWTQDDKLRASSFKGLRPDKPITDARREHPVDSPS"
     gene            2251..2943
                     /locus_tag="VFO47_08925"
     CDS             2251..2943
                     /locus_tag="VFO47_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788858.1"
                     /translation="MGAHILVVDDDPLLAAALRRPLAYEGFEVEVAASGEEALTRALD
                     HAPDLVILDVLLPGIDGLEVCRRLRQAGEVAVLMLTARSEVPERVAGFEAGADDYLVK
                     PFAFEELLVRVKALLRRGRNGAGTAELRFGDLRMDRSTHEVWRGERTISLTRQEFDLL
                     SLFLEHPRQVLTRSTILERVWDYDFGRDSNVLEVYVGYLRRKLEAEGEQRVIHTVRGV
                     GYVLREDPEGGR"
     gene            2940..>4019
                     /locus_tag="VFO47_08930"
     CDS             2940..>4019
                     /locus_tag="VFO47_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET8788859.1"
                     /translation="MTFRTRLILAYLVLLTTAVSGFGLWVYAYVDRNLHQEFYGSVQR
                     QSNQLAQVLASDYDNSASRAGDILDQWTNEQQRDTYIVVETRKDPELRLEIEAKRPKD
                     ALGGVDLPVVTPGRVVNVRPSANDLGLPLALYAEWFEARRAVPRRSATVADQPSEIVK
                     LQGQVTVARSLAGVERSLRLLRTILIAGGLAVLLVAALVGLGLAAALLRPLARMRATA
                     QRIGDERDFSHRMPVDGDPHDELGRLSLSFNQMLAELEQANLGLKTTLDSQRRFVADA
                     SHELRTPLTAIRTNVEFLARVPGARSEDRVEALRDVLTELRRMESLVGDLLALARLEA
                     AARPARRTFRFDHMLADVHRDALRAA"
BASE COUNT          567 a         1525 c         1404 g          523 t
ORIGIN      
        1 ctacgaggac gtccggaccg tggccaagct gatcaaggtg gtcctcgacg gcctcggcct
       61 gcgcggctac gccaagacgt cgggggcgac cggcatgcag gtgttcgtgc cgctggacgg
      121 cacccacacc cacagcgacg cccgcgaggt cgtggagcgg gtgggccggc tggtcgtccg
      181 cgcctacccg gagaaggcga ccatggcctg gccggtcgcc gaccgcgccg gcaaggtctt
      241 catcgaccac cagatgaacc ggtcgggggc caacatcgcc agcgtctact cgctgcggcc
      301 gctgccgggg gggccggtgt cgaccccgct ggactgggac gagctggaca ccgacatcga
      361 gccgggcgac ttccggatcg acaacgtctg ggagcggttc gccgccgggg accggttcgc
      421 ggcggtcctg gacgacaagc agtccctgac ccccgccatg gaggccctcg gcctcacccc
      481 cggcgccccc gagcctcccc gggaccgcag ccgcccgagg ttccccccca ggcgcaccac
      541 ccgcccggcc tcgcgggcca gcctggacga gtacgtggcc aaacgcgact tcagtcgcac
      601 cccggaaccc cccacctcgg tggacgggcc cccgaacggg agggtggccc gcagcgggga
      661 cgagggcgaa cggctgtcca cagcccccac cgaccccaca gcccccaccc ccaccgaccc
      721 cgctccaacc ggccccatga cctccacggg gctgccggag gaaccgcatc cggcggggtt
      781 gacggaggtg gcggcgctcg aacccgggcg gcggttcacc atccagcagc accatgcgcg
      841 gcggctgcac cacgacgtgc ggttcgagca ggacgaggtg ctggtctcgt tcgccgtgcc
      901 caagcggctg cccgaggaac cggggctgcg ccacctggcc gtgaacaccg aggaccaccc
      961 gctcgactac ctgaccttca gcgggacgat cccggccggc gagtacggcg ggggcgaggt
     1021 ccgcctgttc gacctcggca cctacgagcc gctggaggtg gccgacggca agctcaccgt
     1081 ccggctccac gggagccgct acaagggtgc cgagtaccac ctgttccgga cccgggaccg
     1141 cgactggctg gtcatcctca agggcaaggt gtcgctcccc cagccggccc cgccgccgac
     1201 ctacgagccg atgatggccg tgctcaccgc cgaggcgttc gacgacgacg actggctgtt
     1261 cgaggtcaag tgggacggtc accgctgcct ggccaacctg ggcacctcga cccgcctgac
     1321 ctcgcggacg acccgcgaca tgacggccca gttccccgag ctgatcgaca tgcaccgcca
     1381 gctggcggcc cgcaacgcgg tcgtcgacgg cgagatcgtg gccctcgacc ggaacggccg
     1441 gccatcgttc gagcgcatgc aggaccggtt ccaccgcacc cccgacgagc tggcccggaa
     1501 caggggccgg gtgccggtcc agttcctcgc cttcgacctg ctgtggctgg acggccagtc
     1561 cctgctcgac ctgccgctgg cggagcggcg gacccggctg gtcgaggtgc tggtcgagac
     1621 cagggacatc cgcctctccc aggtcgtcga gggcgccggc aaggcgttct tcgaccaggt
     1681 caaggcgctg cagctggagg ggatcgtggc caagcgggcg gccagcccct accggccggg
     1741 cacccgctcg cacgactggc gcaagatcaa ggccctctgc ctccaggact gcgtgatcgt
     1801 cggctggacg cccggcaagg gcggccgggc cgccaccctg ggctcgctgc tgctggcggt
     1861 gtacgacggc gggcggctgc gctacgccgg caacgtcggc accggcttca cccacgcctt
     1921 cctggccgac ctgctggcca ggctgcagga gctccgggtc gacaagcccc agttcgaggg
     1981 cttcgagggc acgttgcggc cgcgcggggc ccgcttcgtc cggcctgagc tggtctgcga
     2041 ggtcgagtac ctgaagtgga cccaggacga caagctgcgg gcgtcgtcgt tcaagggcct
     2101 ccggcccgac aagccgatca ccgacgcgcg ccgcgagcac cccgtggaca gcccaagcta
     2161 ggcagttgcg ccgtcgagca ggcagaatgg cggcaaggca gcacgcgacg atcgccgccg
     2221 gacggcggcc ggttccacgg cggtaggtgc atgggcgcac acatcctggt cgtcgacgac
     2281 gatcccctcc tggccgcggc ccttcgccga ccgctggcct acgaggggtt cgaggtcgag
     2341 gtggccgcct ccggtgagga ggccctcacc cgggccctcg accacgcgcc cgacctggtc
     2401 atcctggacg tgctgctgcc cggcatcgac ggcctggagg tgtgccggcg gctgcgccag
     2461 gccggtgagg tggccgtgct catgctgacc gcccgctccg aggtgcccga gcgggtggcc
     2521 gggttcgagg ccggcgccga cgactacctg gtcaagccgt tcgccttcga ggagctgctg
     2581 gtccgggtca aggccctgct gcggcggggc cgcaacggcg ccggcacggc cgagctgcgc
     2641 ttcggcgacc tgcgcatgga ccgctccacc cacgaggtct ggcggggcga gcggaccatc
     2701 tcgctgaccc gccaggagtt cgacctgctg tccctgttcc tggagcaccc gcgccaggtc
     2761 ctgacccgct cgaccatcct ggagcgggta tgggactacg acttcggccg cgactccaac
     2821 gtgctggagg tctacgtcgg ctacctgcgc cgcaagctcg aggccgaggg cgagcagcgg
     2881 gtcatccaca ccgtccgcgg ggtcggctac gtcctccgcg aggacccgga gggcggccgg
     2941 tgaccttccg gacccgcctc atcctggcct acctggtcct gctcaccacc gccgtgtccg
     3001 ggttcgggct gtgggtctac gcctacgtcg accgcaacct ccaccaggag ttctacggct
     3061 cggtgcagcg ccagagcaac cagctggccc aggtgctggc cagcgactac gacaactccg
     3121 cctcccgggc cggcgacatc ctcgaccagt ggaccaacga gcagcagcgc gacacctaca
     3181 tcgtggtcga gacccgcaag gacccggagc tgcgcctgga gatcgaggcc aagcggccca
     3241 aggacgccct cggcggcgtc gacctgccgg tcgtgacgcc cggccgggtc gtcaacgtcc
     3301 ggccgtcggc caacgacctc ggcctgcccc tggccctgta cgccgagtgg ttcgaggccc
     3361 gcagggccgt cccccggcgc agcgcgaccg tggccgacca gcccagcgag atcgtcaagc
     3421 tccagggcca ggtgaccgtg gcccgctccc tggccggggt cgagcggtcc ctgcggctgc
     3481 tgcggaccat cctgatcgcc ggcgggctgg ccgtgctgct ggtcgccgcc ctcgtcggcc
     3541 tcggcctggc cgcggccctg ctgcggcccc tggcccgcat gcgggccacc gcccagcgga
     3601 tcggcgacga gcgcgacttc tcccaccgca tgccggtcga cggcgacccg cacgacgagc
     3661 tgggccgcct gtcgctgtcg ttcaaccaga tgctggccga gctggagcag gccaacctgg
     3721 ggctgaagac gacgctcgac tcccagcgcc gcttcgtcgc cgacgcctcc cacgagctgc
     3781 gcacccccct gaccgcgatc cgcaccaacg tcgagttcct ggcccgggtc cccggcgccc
     3841 ggagcgagga ccgggtcgag gccctgcgcg acgtgctcac cgagctgcgg cggatggagt
     3901 cgctggtcgg cgacctgctg gccctggccc ggctggaggc ggcggcgcgg ccggccaggc
     3961 ggaccttccg gttcgaccac atgctggccg acgtgcaccg cgacgccctg cgggcggcc
//
LOCUS       DASSWR010000532         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_980188,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000532 DASSWR010000000
VERSION     DASSWR010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980188"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFO47_08935"
     CDS             <1..304
                     /locus_tag="VFO47_08935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET8788860.1"
                     /translation="DVEDWLAVAATARRMARWVVGAFKAGGVDLVQANSDGSVGNQTV
                     FHLHVHVLPRYHDDQLGSWWSPQTADPAEITEAARRLVEHGRSDPERIPPLASTST"
     gene            378..974
                     /locus_tag="VFO47_08940"
     CDS             378..974
                     /locus_tag="VFO47_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788861.1"
                     /translation="MDTGLLVAILVVLVLLALLAFFAGRQRRSRKLQDTFGPEYQRTV
                     EETGDRKAAEAELVERTERRAGFEIVPLEPEARARYVEAWRHTQAQFVDEPAEATREA
                     DRLITSVMRDRGYPVDDFDQRAADLSVDHPQVVDDYRAAHAIAARNERSEASTEDLRQ
                     ALVHYRSLFEELLDDRHPDGSVPEPDRHPDRSTTEEAR"
     gene            971..1783
                     /locus_tag="VFO47_08945"
     CDS             971..1783
                     /locus_tag="VFO47_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788862.1"
                     /translation="MTERYGSGYGDEGAQRPSGRGAVTERLARRDQDAREIDLTDEAA
                     KAEADDDTVVSPAARPDGDQADRESSGAFEPAGPVAPATDPPAEAGRTGSSIFDVDER
                     AKQEELNRTGYEPPVADTADPDGSRGETTVTEPGEPRREPADAEASDATAAPVADPPA
                     EADADTPADAAPAPGGGAAAEAPEALLGSLNAGEIRGRFLDIQAGFIDEPRQAVEEAG
                     RFVDDLVRQVADALQQQRGQLAGASDDASTEDLRLTLRAYRRFVDRLLGLGG"
     regulatory      1905..2019
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2010..>2370
                     /locus_tag="VFO47_08950"
     CDS             2010..>2370
                     /locus_tag="VFO47_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HET8788863.1"
                     /translation="MTAGQRSFSIQQPPEWGIEPVPPGQRRLGFLDQAVLWGNLGVSL
                     LVLVAGALLVPALGLWQALAATVVGAVVGNALLGLAAVPAAATGVPAMVLYRAPLGIR
                     GSLLPTACNVVQNLGWAT"
BASE COUNT          322 a          914 c          865 g          269 t
ORIGIN      
        1 cgacgtcgag gactggctgg ccgtggcggc cacggccagg cggatggccc gctgggtggt
       61 cggggccttc aaggccggcg gggtcgacct ggtgcaggcg aactccgacg gctcggtcgg
      121 aaaccagacc gtgttccacc tgcacgtcca cgtgctcccg cgctaccacg acgaccagct
      181 cgggtcctgg tggtcgccgc agacggccga cccggccgag atcaccgagg ccgcccgccg
      241 cctggtcgag cacggccgct ccgaccccga gcgcattccg ccgctggcct ccacctccac
      301 ctgagcgtcg gtcgcaggtt tgcgtcccgg cttgaacggg atagcgaaac aagagacgct
      361 ggggaaaggg gacacccatg gacacaggtc tgctcgtcgc catactcgtc gtgctcgtgc
      421 tgctggcgct gctcgccttc ttcgccggcc gccagcgccg cagccgtaag ctgcaggaca
      481 cgttcggccc ggagtaccag cgcaccgtgg aggagaccgg cgaccgcaag gcggccgagg
      541 ccgagctggt cgagcggacc gagcgccgcg ccgggttcga gatcgtcccc ctggagcccg
      601 aggcccgggc ccgctacgtc gaggcctggc gccacaccca ggcccagttc gtggacgagc
      661 ccgccgaggc cacccgcgag gccgaccggc tgatcacctc ggtgatgcgc gatcgcggtt
      721 acccggtcga cgacttcgac cagcgggccg ccgacctgtc ggtcgaccat ccccaggtgg
      781 ttgacgacta ccgggccgcc cacgccatcg ccgcccgcaa cgagcgttcc gaggccagca
      841 ccgaggacct gcgccaggcc ctggtccact accggtcgct gttcgaggag ctgctggacg
      901 accgccaccc ggacgggtcc gtgcccgagc cggatcgtca tcccgaccgg tccacgaccg
      961 aggaggcacg atgacggagc gctacgggtc cggctacggc gacgagggcg cccagcggcc
     1021 gtcgggccgc ggggccgtga ccgagcggct ggcccggcgg gaccaggacg cccgcgagat
     1081 cgacctgacc gacgaggcgg ccaaggccga ggcggacgac gacaccgtgg tcagcccggc
     1141 cgcccggccc gacggcgacc aggccgaccg cgagtcgtcc ggcgccttcg agccggccgg
     1201 gccggtggca ccggccaccg acccgccggc cgaggccggc cggacgggct cgtccatctt
     1261 cgacgtcgac gagcgggcca agcaggagga gctcaaccgg accggctacg agcccccggt
     1321 ggccgacacc gccgatccgg acggttcgcg cggcgaaacc accgtcaccg agcccggcga
     1381 gccccgccgc gagcccgccg acgccgaggc cagcgacgcc accgcggccc cggtcgccga
     1441 ccctcccgcc gaggccgacg ccgacacgcc agccgacgcc gcacccgccc cgggcggtgg
     1501 ggccgccgcc gaggcgccgg aggcgctgct cgggtcgctg aacgcgggcg agatccgcgg
     1561 ccggttcctc gacatccagg ccgggttcat cgacgagccc aggcaggcgg tcgaggaggc
     1621 cggccgcttc gtcgacgacc tcgtccggca ggtggccgac gccctccagc agcagcgcgg
     1681 ccagctcgcc ggcgcgagcg acgacgcctc gaccgaggac ctgcgcctga ccctgcgcgc
     1741 ctaccggcgg ttcgtcgacc ggctcctggg cctgggcggc tgagcgagac gctcagcgtc
     1801 ggggtcgcgg aggagtacca gctcgtcgac ccggcgaccc gcgggaggcg gtggtggatc
     1861 tggtggccga ggcgaccgcc cgctaaggtg gccgcgggcc ttcgacggag gtgccagggg
     1921 agcccggcgg aaccgggctg agaggccgac tgcagcggtc ggcgaccctt cgaacctgat
     1981 ccggctagca ccggcgaagg gagcgcgaga tgacggctgg ccagcggtcc ttctccatcc
     2041 agcagccccc cgagtggggc atcgagccgg tcccgcccgg ccagcggcgg ctcggcttcc
     2101 tcgaccaggc ggtgctctgg ggcaacctcg gcgtgagcct gctcgtgctc gtcgccgggg
     2161 ccctgctcgt ccccgccctg ggcctgtggc aggccctggc cgccaccgtg gtcggggccg
     2221 tggtcggcaa cgccctgctc gggctggccg ccgtgccggc cgccgccacc ggcgtgccgg
     2281 ccatggtcct gtaccgggcc ccgctcggga tccgcggctc gctgctcccc accgcctgca
     2341 acgtcgtcca gaacctgggc tgggccacct
//
LOCUS       DASSWR010000533         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_613172,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000533 DASSWR010000000
VERSION     DASSWR010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613172"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..759)
                     /locus_tag="VFO47_08955"
     CDS             complement(130..759)
                     /locus_tag="VFO47_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8788864.1"
                     /translation="MGTASTPMSGRRAEAARNDQRILDAARAVFVADPGAPIAAVAEH
                     AGVGISALYRRYASKEDLLRRLCADGLRTYIAAAEEALADDGDPWEAFARFMRRIVDA
                     DTHSLTSRLAGTFTPTEELNRDAAIAQELNERLFERTMASGTIRPDLDVNDLSMVFEQ
                     VASIRMGDQARTSQLRHRYLALLLEAMHGPGDGPLPGPPPTWRELVGRW"
     gene            complement(957..>2025)
                     /locus_tag="VFO47_08960"
     CDS             complement(957..>2025)
                     /locus_tag="VFO47_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET8788865.1"
                     /translation="VVTCHGATAIAEGETVLVDGTEGTVDVGPDDATVQQALDAEQRK
                     LAVLQAVSGPGRTSDGHAVQLLVNIAGDKDLEPAAAADSEGVGLFRTEFLFLDRPNAP
                     TLEEQQAVYQRVFDAFPGRKVVVRTLDAGADKPLEFVTVPDEPNPALGVRGLRTSRRH
                     PELLEEQLTAVAAAASASQAQVWVMAPMVSTTGEAAAFAEQVRGHGLSSGGVMVEVPA
                     AAIRARHVAAACDFMSIGTNDLSQYTYAADRMAGELADLLDAWQPALLDLVRMTAEAG
                     KALGKPVGVCGEAASDPLLALVFVGLGITSLSMAPVSIPAVRAALAAHTLAECRGLAT
                     LALDAEDGRAARTAVRNATRS"
BASE COUNT          250 a          753 c          764 g          258 t
ORIGIN      
        1 ccaccagggg accagcccgg ccacgacgcc cggcgccagc gcgaagaaca ccgcgctgcc
       61 gagggcggca accgccttcc gcatcgcccc agcctacccg ggccgggcgc cggggccgtg
      121 gggcggcagc taccagcggc cgacgagctc ccgccaggtc ggcgggggac cggggagcgg
      181 accgtccccg gggccgtgca tggcctccag gagcagggcc aagtagcggt ggcggagctg
      241 gctggtgcgg gcctgatcac ccatgcggat ggacgccacc tgctcgaaca ccatggacag
      301 gtcgttgacg tcgaggtcgg gccggatggt ccccgacgcc atggtccgct cgaacagccg
      361 ctcgttgagc tcctgggcga tggcggcgtc gcggttcagc tcctcggtgg gggtgaaggt
      421 gccggccagg cgcgaggtca gggagtgggt gtcggcgtcg acgatccggc gcatgaagcg
      481 ggcgaaggcc tcccacgggt caccgtcgtc ggccagggcc tcctcggcgg cggcgatgta
      541 ggtccgcagc ccgtccgcgc agaggcgccg cagcaggtcc tccttgctcg cgtagcgccg
      601 gtacagggcg ctgatgccga ccccggcgtg ctcggccacg gcggcgatcg gcgcccccgg
      661 gtcggccacg aacaccgccc tggcggcgtc gaggatccgc tggtcgttcc gggccgcctc
      721 ggcccggcgc ccgctcatcg gcgtggatgc ggtgcccatg ggagcagctt accactggaa
      781 cgaaccgttc cgttctgctc agtcggccgg cgacgcctcg atgacgctca gtccacgcag
      841 cacccgcgtg agcagctccg gggtccaccc cgaccgcctc ggcggccctc cgcccccggc
      901 gcgggccccc gtgccggtgt cggtgccggt gtcggggcgt cccgtgggcc cgacgctcag
      961 ctccgggtgg cgttgcggac ggcggtgcgg gcggcccggc cgtcctcggc gtcgagggcg
     1021 agggtggcga gcccgcggca ctcggccagg gtgtgggcgg cgagggcggc gcggaccgcc
     1081 gggatgctga ccggggccat ggacaggctg gtgatgccca gcccgacgaa caccagggcg
     1141 agcagcgggt cgctggccgc ctcaccgcac accccgaccg gcttgccgag ggccttcccg
     1201 gcctccgcgg tcatccggac caggtcgagc agggccggct gccaggcgtc gagcaggtcg
     1261 gccagctccc cggccatccg gtcggccgcg taggtgtact gggacaggtc gttggtcccg
     1321 atcgacatga agtcacaggc cgccgccacg tgcctcgccc tgatggccgc cgcgggcacc
     1381 tcgaccatca cgccgcccga ggacagcccg tggccgcgca cctgctcggc gaaggcggcc
     1441 gcctcgcccg tggtcgagac catcggcgcc atcacccaga cctgggcctg gctggccgac
     1501 gcggccgcgg ccacggcggt cagctgctcc tccagcagct cgggatggcg ccgcgaggtc
     1561 cgcagtccgc gcacgcccag ggcggggttg ggctcgtcgg gcacggtgac gaactccagc
     1621 ggcttgtcgg cgccggcgtc cagcgtccgc accaccacct tgcgccccgg gaaggcgtcg
     1681 aacacccgct ggtagacggc ctgctgctcc tcgagagtgg gcgcgttggg ccggtcgagg
     1741 aacaggaact cggtccggaa cagcccgacg ccctcggagt cggccgcagc cgccggctcc
     1801 aggtccttgt cgccggcgat gttgaccagc agctgcactg cgtggccgtc ggaggtgcgg
     1861 ccgggaccgc tgaccgcctg gaggacggcc agcttcctct gctcggcgtc gagcgcctgc
     1921 tggacggtgg cgtcgtcggg gccgacgtcc accgtcccct cggtcccgtc gaccaggacc
     1981 gtctcgccct cggcgatggc cgtggcccca tggcaggtga ccacc
//
LOCUS       DASSWR010000534         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1348059, whole genome shotgun sequence.
ACCESSION   DASSWR010000534 DASSWR010000000
VERSION     DASSWR010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348059"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1349
                     /locus_tag="VFO47_08965"
     CDS             267..1349
                     /locus_tag="VFO47_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788866.1"
                     /translation="MGGGEIRLVELVKRFEDVTAVDGVDLDVPGGEFFALLGPSGCGK
                     TTTLRLVAGFEQPTGGRILLDGVDMARTPPHKRKVNTVFQSYALFPFLNVADNVAFGL
                     RFQDLDKGEARRRVAEALALVQLQGLERRRPAQLSGGQAQRVALARALVLNPSVLLLD
                     EPLGALDAKLRKTLQVELKALQEAVGITFVYVTHDQEEALTMSDRLAVMAGGRIEQVG
                     PPAELYEAPATTYVAGFLGVANLLGATAAGGDGGGPCRVRLGEFELAAAGGDTGASGD
                     VKVVIRPERVVLEPYGTTGPNRVPGMVERLVYLGPSTQLVVRLANGEPLQALVQNQGQ
                     PIAWQQGTAIAVHLPADALRVLPASA"
     gene            complement(1341..2003)
                     /locus_tag="VFO47_08970"
     CDS             complement(1341..2003)
                     /locus_tag="VFO47_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788867.1"
                     /translation="MSPRRKLSDARRHQILKAAVRVIAEKGLCDTGIKDVADQAGTSP
                     ALVIYYFGTKDTLLAEALAFAEDRFYAKTAEAVAGMSSARDRLVELVRCSCSVGEAED
                     DFDEWVLWLDLWARAAHKPDVARDRLTMDRRWRTTIGEIVRQGQAAGEFAPVDVETFA
                     LRLAALIDGLAILVVLQDPEVSRERMFDLCLEICARELGFDWTAEDRASLVPSPERAA
                     SG"
     gene            2119..3735
                     /locus_tag="VFO47_08975"
     CDS             2119..3735
                     /locus_tag="VFO47_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8788868.1"
                     /translation="MAATWDAVVIGGGHNGLVAGAYLARAGARTVVLEARSKVGGAAA
                     TDAPWPEAPEINVTRLSYVMSLMPPRIIRDLDLERYGYKLFPMGPAYQAWPDGRSLTL
                     YDDARRNHEQVSRFSKRDADAMVRWDAWLEGLAAVLGPLLTQTPPRLGSRRPSDLRDL
                     LRLAWRHRGLDVRTVGDVTRLMTMSVRDLLDDWFESEEVKASLAINGVIGTWGGPDSP
                     GTAYVMAHHSIGDVGDGHLGSWGFPEGGMGAVSDAIHRSALANGASVRTGARVERVLV
                     SGGRATGVALTGGEVLHAPVVVTAIHPRITFLEQVDRAELPAEFVTDMERWKSRSGVV
                     KINLALAELPDFTADPGTEVAEHHTGAIAMALSVDYIEQAFQDAKQGRGATRPFSDGC
                     IPTVFDKTLAPEGIHVMSLFTQWCPHEWSEAPHADELEAYADRMIDCYAELAPNLKGA
                     ILHRDVVGPWQMEQEWGLVGGNIFHGELSVEQLFHMRPAPGYADYRSPITGLYQASSA
                     THGGGGVCGIPGWQAFRAIQADRKRAIRRR"
BASE COUNT          511 a         1369 c         1360 g          513 t
ORIGIN      
        1 cgaccgctgg agccactgcc actggctcta ccagcacaac ggcggcgtgt tcctgccgcc
       61 ctggggcaag gccgagcagt ggctgctgtc cgcccagcac ggcgacgagg acgtcgaccg
      121 gttcgtggcc aacttcgccg ccttcgccgg cgacgtcgcc ggcggctgag gtgctgaccg
      181 tgccacccgg cacggccctg agctgatccg gcggccacgg ccggttacga tgccggcagc
      241 ggagcgtggc ggagaggggt ggccgcatgg gcggaggcga gatccggctg gtcgagctgg
      301 tcaagcggtt cgaggacgtc accgccgtcg acggcgtcga cctggacgtg cccgggggcg
      361 agttcttcgc cctgctcggc ccttcgggct gcggcaagac gaccaccctg cggctggtcg
      421 ccgggttcga gcagcccacc ggcgggcgca tcctcctcga cggggtcgac atggcccgta
      481 ccccgcccca caagcgcaag gtcaacaccg tgttccagag ctacgccctg ttcccgttcc
      541 tcaacgtggc cgacaacgtc gccttcgggc tgcggttcca ggacctggac aagggcgagg
      601 ccaggcggcg ggtggccgag gccctggccc tggtccagct ccaggggctg gagcggcggc
      661 ggccggccca gctgtcaggc ggccaggccc agcgggtcgc gctcgcccgg gcgctggtcc
      721 tgaacccgtc ggtgctgctg ctggacgagc cgctgggggc cctggacgcc aagctgcgca
      781 agaccctcca ggtggagctg aaggcgctcc aggaggcggt cgggatcacc ttcgtgtacg
      841 tgacccacga ccaggaggag gccctcacca tgtcggaccg cctggccgtg atggccggcg
      901 gccgcatcga gcaggtcggt cccccggccg agctgtacga ggcgccggcg accacgtacg
      961 tggccgggtt cctgggggtg gccaacctcc tgggggcgac ggccgccggt ggcgacggcg
     1021 gggggccctg ccgggtccgg ctgggcgagt tcgagctggc cgcggccggc ggcgacaccg
     1081 gcgccagcgg cgacgtcaag gtggtcatcc ggcccgagcg ggtggtcctg gagccttacg
     1141 gcacgaccgg ccccaaccgg gtcccgggca tggtcgagcg gctggtctac ctggggccgt
     1201 ccacccagct ggtggtccgc ctggccaacg gcgagcccct ccaggcgctg gtccagaacc
     1261 agggccagcc gatcgcctgg cagcagggca cggccatcgc cgtccacctc cccgccgacg
     1321 ccctgcgggt cctgcccgcc tcagcctgag gcggcccgct cgggcgaggg caccaggctg
     1381 gcgcggtcct cggccgtcca gtcgaagccg agttccctgg cgcagatctc caggcacagg
     1441 tcgaacatgc gctcgcggga cacctccggg tcctggagga cgaccaggat ggccaggccg
     1501 tcgatcaggg cggccagccg cagggcgaac gtctccacgt cgaccggggc gaactcgccc
     1561 gccgcctgcc cctggcggac gatctcgccg atggtcgtcc gccagcgccg gtccatggtc
     1621 aggcggtccc tggccacgtc cggcttgtgg gccgccctgg cccacaggtc gagccacagc
     1681 acccactcgt cgaagtcgtc ctcggcctcc ccgaccgagc acgagcagcg caccagctcg
     1741 accagccggt cgcgggcgct ggacatgccg gccacggcct cggccgtctt ggcgtagaag
     1801 cgatcctcgg cgaaggccag cgcctcggcg agcagggtgt ccttggtgcc gaagtagtag
     1861 atgaccaggg ccgggctggt gccggcctgg tcggccacgt ccttgatccc ggtgtcgcag
     1921 aggcccttct ccgcgatcac ccgcacggcc gccttgagga tctggtgccg ccgtgcgtcg
     1981 gacagcttgc gtcgtgggct catgcctgct ccaaggatgt cgacgtcgcc gtgcagtgta
     2041 gggcgcttca ggcttgtcgc gcgctccccg gccatcttag actgaacgtt cagtcaataa
     2101 cagtcttttg gaggggacat ggccgcgacc tgggacgccg tcgtcatcgg gggcggccac
     2161 aacgggctgg tggccggcgc ctacctggcc cgggcggggg cgcgcacggt cgtcctggag
     2221 gccaggtcca aggtcggggg cgcggccgcc accgacgccc cctggcccga ggcccccgag
     2281 atcaacgtga cccggctgtc gtacgtgatg agcctgatgc cgccgcggat catccgcgac
     2341 ctcgacctgg agcggtacgg ctacaagctg ttccccatgg gcccggccta ccaggcctgg
     2401 ccggacggcc gctcgctgac cctgtacgac gacgcccgcc gcaaccacga gcaggtctcg
     2461 agattttcca agcgggacgc cgacgccatg gtccgctggg acgcctggct ggaggggctg
     2521 gcggcggtgc tggggccgct gctcacccag accccgccca ggctcggctc gcgccggccg
     2581 tcggacctgc gcgacctgct ccggctggcc tggcgccacc gcggcctgga cgtgcgcacc
     2641 gtcggcgacg tgacccggct catgaccatg agcgtccgcg acctgctgga cgactggttc
     2701 gagtccgagg aggtcaaggc gagcctggcc atcaacgggg tgatcggcac ctggggcggg
     2761 cccgactcgc ccggcaccgc gtacgtgatg gcccaccact cgatcggcga cgtcggcgac
     2821 ggccacctcg gcagctgggg cttccccgag ggcggcatgg gggcggtctc ggacgccatc
     2881 caccgctcgg ccctggccaa cggggccagc gtgcgcaccg gcgcccgggt cgaacgggtg
     2941 ctggtgtcgg gcgggcgggc caccggggtg gccctgaccg gcggggaggt gctgcacgcc
     3001 ccggtggtgg tgacggccat ccacccccgg atcacgttcc tggagcaggt cgaccgggcc
     3061 gagctgccgg ccgagttcgt caccgacatg gagcgctgga aatcgcgcag cggggtggtc
     3121 aagatcaacc tggccctggc cgagctgccc gacttcaccg ccgaccccgg caccgaggtg
     3181 gccgagcacc acaccggggc catcgccatg gccctgtcgg tcgactacat cgagcaggcg
     3241 ttccaggacg ccaagcaggg ccggggcgcg acccggccgt tctcggacgg ctgcatcccg
     3301 acggtgttcg acaagaccct ggcccccgag ggcatccacg tcatgtcgct gttcacccag
     3361 tggtgcccgc acgagtggtc ggaggctccc cacgccgacg agctggaggc ctacgccgac
     3421 cggatgatcg actgctacgc ggagctggcc cccaacctca agggcgccat cctgcaccgc
     3481 gacgtcgtcg ggccctggca gatggagcag gagtgggggc tggtcggggg caacatcttc
     3541 cacggcgagc tctcggtcga gcagctgttc cacatgcggc ccgcccccgg ctacgccgac
     3601 taccgcagcc cgatcaccgg cctctaccag gccagctcgg ccacccacgg cggcggcggg
     3661 gtctgcggca tccccggctg gcaggcgttc cgggccatcc aggccgaccg caagcgggcc
     3721 atccggcgca ggtgaacctc accgctctgc tgg
//
LOCUS       DASSWR010000535         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1403692, whole genome shotgun sequence.
ACCESSION   DASSWR010000535 DASSWR010000000
VERSION     DASSWR010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403692"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..785
                     /locus_tag="VFO47_08980"
     CDS             120..785
                     /locus_tag="VFO47_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8788869.1"
                     /translation="MTEAGSEPAGSAPTRRAVLDLLKRNGSQTVAQLGAALNLSGVAV
                     RRHLETLEHVGLVRQTTRPQGRGRPAYVYALTEMGHDLFPRNYHQLVAQLLEAATSEL
                     GPEAVERLFDHRQRELAELYAGRTSGRPLPELARALAAIQDENGYMADCASDGDGRFV
                     VSEHNCAIARVAGAWPTACAHELALFRQLAGPDVEVERVAHIQAGDPVCAYVLRAAQL
                     NGR"
     gene            792..1874
                     /locus_tag="VFO47_08985"
     CDS             792..1874
                     /locus_tag="VFO47_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018811728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HET8788870.1"
                     /translation="MVRIRNGRVTAIGDRRPGATELRVALDGEDGEVPAISYDRLTGP
                     VEAGDEVVLNTTAVALGLGTGGFHVVMARVGGPVDDPGPGHVMKARYTPSQVRVLAVE
                     EEDSPHRAAVAACTDLGGLPVVCAELHSMVPVVAAAARAVAADLRVGYVMTDGGALPL
                     AFSRLAAELRAGGLVAGTITAGQAFGGDLEAISLYSALAAARAVLAADVVVVAQGPGG
                     MGSGTALGFSGTQVAEAVNAAAALGGRPVACLRLSAADRRPRHRGVSHHSLTALGRLA
                     LARSAVAVPELADPDLAGLVDRQLAEAGVAGRHDLHRVAIPDPAKLLAGWGLQVTSMG
                     RGPEDDPVFFAAAAAAGTLAGRLARP"
     gene            1871..>2881
                     /locus_tag="VFO47_08990"
     CDS             1871..>2881
                     /locus_tag="VFO47_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788871.1"
                     /translation="MSGGVRGTAGVIPRWIGPVSRLSDRAVFTACLGGLVAGIGLFLV
                     LPLAPQFLELHRLPTNRDFDVELRPQGQVTAFWVVSMASFALAVWQWRRGRRPGWALL
                     LAGVAVLHLLALLVPPVASKDVYAYSFYGKVQTTYGENPYLSFPDQHPTDPWHKFWSW
                     RLFGPVYGPPFLLLLRLLATAAGPSLLTWVVLTKLVLVAAELAAIWVLVRALRAAPSL
                     GEDPRWPLLLLGWNPMVLQSIAMSAHVDALLLLVLALAILAHRRGRHLLAFLALVGCF
                     LIKVYMGPLAALYAIWLATGRPRLRDRVAVVAALGLLGTVLTVLVYLPYASAGAKLFT
                     SAV"
BASE COUNT          296 a         1120 c         1074 g          391 t
ORIGIN      
        1 agcagggcga tcaggttgac gagccgctcc accttgcgca tgccccgatg ctagtggaat
       61 cgcgcccagc ccggggggcg gcgttgcccc ggctggaacg acagcgctag cctgggccga
      121 tgaccgaggc aggaagcgag ccagcgggga gcgccccgac ccgacgggcc gtgctcgatc
      181 tgctcaagcg caacggctcc cagacggtcg cgcagctcgg cgccgccctg aacctgtccg
      241 gggtggcggt ccgccgccac ctggagacgc tggagcacgt cgggctggtc cgccagacga
      301 cccggcccca gggccgcggc cggccggcct acgtgtacgc cctcaccgag atgggccacg
      361 acctgttccc ccgcaactac caccagctgg tcgcccagct gctggaggcg gccacctcgg
      421 agctggggcc ggaggcggtg gagcggctgt tcgaccaccg ccagcgggag ctggccgagc
      481 tgtacgccgg ccgcacctcc ggccgccccc tgcccgagct ggcccgggcc ctggccgcca
      541 tccaggacga gaacggctac atggccgact gcgccagcga cggcgacggc cgcttcgtgg
      601 tcagcgagca caactgcgcc atcgcccggg tggccggcgc ctggccgacc gcctgcgccc
      661 acgagctggc cctgttccgg cagctcgccg ggccggacgt cgaggtcgag cgggtcgccc
      721 acatccaggc cggcgacccc gtctgcgcct acgtcctccg ggcggcccag ctgaacgggc
      781 gctgagccgc cgtggtccgc atccgcaacg ggcgggtcac ggccatcggc gaccgccgcc
      841 cgggcgcgac cgagctgcgg gtcgccctcg acggcgagga cggcgaggtc ccggcgatca
      901 gctacgaccg gctcaccggg ccggtcgagg ccggggacga ggtggtgctg aacaccaccg
      961 cggtcgccct ggggctcggc accggcggct tccacgtggt catggcccgg gtcggcgggc
     1021 cggtcgacga ccccggcccc gggcacgtca tgaaggcccg ctacaccccg tcgcaggtcc
     1081 gggtcctggc cgtcgaggag gaggacagcc cccaccgggc ggcggtggcc gcctgcaccg
     1141 acctgggcgg cctgccggtg gtctgcgccg agctgcactc gatggtgccg gtggtggcgg
     1201 cggccgcccg ggccgtcgcc gccgacctcc gggtgggtta cgtgatgacc gacggcgggg
     1261 ccctgccgct ggccttctcg cggctggccg ccgagctgcg cgccggcggg ctggtcgccg
     1321 ggaccatcac cgccgggcag gcgttcgggg gcgacctgga ggcgatctcg ctgtactcgg
     1381 ccctggccgc cgcccgggcg gtgctggccg ccgacgtggt cgtggtcgcc caggggccgg
     1441 gcgggatggg gtcgggcacg gccctcgggt tctccggcac ccaggtggcc gaggcggtca
     1501 acgccgccgc cgccctcggc ggccggccgg tggcctgcct gcggctctcg gccgccgacc
     1561 ggcggccccg gcaccgcggg gtcagccacc acagcctgac cgcgctgggc cgcctcgccc
     1621 tggcccgctc ggccgtggcc gtgcccgagc tggccgaccc cgacctggcc gggctggtcg
     1681 accgccagct ggccgaggcc ggggtggccg gccgccacga cctgcaccgg gtggccatcc
     1741 ccgacccggc caagctgctg gccggctggg gcctccaggt gacctccatg ggccgcgggc
     1801 ccgaggacga cccggtgttc ttcgccgccg cggccgccgc cggcaccctg gccgggcgcc
     1861 tggcccggcc atgagcgggg gggtccgggg gaccgccggg gtgatccccc ggtggatcgg
     1921 gcccgtgtcg cggctgagcg accgggcggt gttcaccgcc tgcctgggcg ggctggtcgc
     1981 cgggatcggg ctgttcctgg tcctgcccct ggccccgcag ttcctggagc tgcaccggct
     2041 gccgaccaac cgggacttcg acgtcgagct gcggccccag gggcaggtga cggcgttctg
     2101 ggtcgtgtcc atggccagct tcgccctggc cgtctggcag tggcgccgag gccggcggcc
     2161 cggctgggcg ctgctgctgg ccggggtggc cgtgctccac ctgctcgccc tgctggtccc
     2221 gccggtggcc tccaaggacg tctacgccta ctccttctac ggcaaggtcc agaccaccta
     2281 cggcgaaaac ccgtacctgt cgttccccga ccagcacccc accgacccct ggcacaagtt
     2341 ctggtcgtgg cggctgttcg gccccgtcta cgggccgccg ttcctgctgc tgctgcggct
     2401 gctggccacg gccgccgggc cgagcctgct cacctgggtg gtgctgacca agctggtcct
     2461 ggtcgcggcc gagctggccg ccatctgggt gctggtccgc gccctgcggg ccgccccctc
     2521 gctcggcgag gacccgcgct ggccgctgct gctgctcggc tggaacccga tggtgctcca
     2581 gtcgatcgcc atgtcggccc acgtcgacgc cctgctgctg ctggtcctgg ccctggccat
     2641 cctggcccac cggcggggcc ggcacctgct ggcgttcctg gccctggtcg gctgcttcct
     2701 gatcaaggtc tacatggggc cgctggccgc cctgtacgcc atctggctgg ccaccggccg
     2761 gccccgcctc cgggaccggg tggcggtggt ggccgccctt gggctgctgg gcacggtcct
     2821 gaccgtgctc gtctacctcc cgtacgccag cgccggcgcc aagctgttca ccagcgccgt
     2881 c
//
LOCUS       DASSWR010000536         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1178862, whole genome shotgun sequence.
ACCESSION   DASSWR010000536 DASSWR010000000
VERSION     DASSWR010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178862"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..370)
                     /locus_tag="VFO47_08995"
     CDS             complement(152..370)
                     /locus_tag="VFO47_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788872.1"
                     /translation="MLVKALHAAGLRSTYLQAASLGSIALCLGLWVRAKTVDQDHRDN
                     AERRALFVGLWPPMFWLIGDSLQELEEG"
     gene            436..1155
                     /locus_tag="VFO47_09000"
     CDS             436..1155
                     /locus_tag="VFO47_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HET8788873.1"
                     /translation="MGAETGPARRAGTVARWPLGMGLAFWRYLWRTMPLHRSDEDGSP
                     ERDLPPPLPGGLADGRLQRLEDGSGPLFRRRYRVRIEGGRLTPEELMGELLREPNRAA
                     PIEVAVFRKTRGGEGPLAVGDEFVVRMPGPWDGPVVVADRTPTSFRFATLQGHLEAGQ
                     IEFRTSADGDAVRFEIESWARSGDRLSSLLFDRVKLAKEMQLHMWTHFCERAAKLAGG
                     RIRGGIHIDTRRVEVAGARGR"
     gene            1145..1816
                     /locus_tag="VFO47_09005"
     CDS             1145..1816
                     /locus_tag="VFO47_09005"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HET8788874.1"
                     /translation="MAADRPRAGRRAYRALAALAGKDVNFDPGQSEHFTTENGWKIDD
                     YLQELPPEAPGPPVPGGSFEVAQRLMRDYEFADPAIVRAVYAEDSPFETRDMLLEGRF
                     YGLRFHFGVRVGGLVDEEVAVGGRPVRRWGWNYRTLQGHLEMGQMDYEVRKWLDSGEV
                     DFRIHAFSRPAHIPNPVIRLGFRVFGRWVQRRFARHACQRMARLTAARVAPTAPAGDP
                     PATSR"
     gene            2001..>2616
                     /locus_tag="VFO47_09010"
     CDS             2001..>2616
                     /locus_tag="VFO47_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET8788875.1"
                     /translation="MPGSFAHLHVHTEYSMLDGAARVDDLFAEAARMGMPALAMTDHG
                     QLFGAVDFYLAGQRHGVKPVIGCLLAGQEIITADGVKNVEDVRVGDMVLTHNGRFRRV
                     LRTMRRPYEGQAYTVSLGGRYGRTLTLTEEHPILVRTRDGLVDWTKPADIDAGQASKH
                     GGVNCWKSWACLPRLRTEWTEIRSSDLLPDDFSAPLTGGIARTYR"
BASE COUNT          385 a          926 c          927 g          378 t
ORIGIN      
        1 gaccgggccg cctacaccga cgccaagacc ccgctcatcg acgctttgct ggcgggatcg
       61 gcttgtccat caggatcgac tccgactcga gcttgtcgca gcgccagggg cggacctcgc
      121 cggtctcggt gaagcccagg cggcgccaga atcagccctc ctcgagctcc tggagggagt
      181 cgccgatcag ccagaacatc gggggccaga ggccgacgaa cagggcccgg cgctcggcgt
      241 tgtcgcggtg gtcctggtcg accgtcttgg cccggaccca gaggccgagg cagagggcga
      301 tcgagccgag cgacgccgcc tgcaggtagg tgctgcgcag gccggcggcg tggagtgcct
      361 tgaccagcat cgtggctcct tctcgatggt ctatggggca accgttgggg tagacgacca
      421 cgaaaaggga gaaacatggg agcggagacg gggccggcca ggcgggccgg gacggtggcc
      481 aggtggccgc tcgggatggg gctggcgttc tggcgctacc tgtggcggac gatgccgctg
      541 caccgcagcg acgaggacgg gtcgccggag cgggacctgc cccctcccct gcccggcggg
      601 ctggccgacg ggcggctcca gcggctcgag gacgggtccg ggccgctgtt ccggcgccgc
      661 taccgggtcc gcatcgaggg cggccggctc acccccgagg agctgatggg cgagctgctg
      721 cgcgagccca accgggccgc cccgatcgag gtggccgtgt tccgcaagac cagagggggg
      781 gaggggccgc tggccgtggg ggacgagttc gtggtccgca tgcccggccc ctgggacggc
      841 ccggtggtgg tggccgaccg gacgccgacc tcgttccggt tcgccaccct ccagggccac
      901 ctggaggccg ggcagatcga gttccggacc tcggccgacg gcgacgccgt ccgcttcgag
      961 atcgagtcgt gggcccgcag cggcgaccgc ctgtcgagcc tgctgttcga ccgggtcaag
     1021 ctggccaagg agatgcagct gcacatgtgg acccacttct gcgagcgggc ggccaagctg
     1081 gccggcggcc gcatccgcgg cggcatccac atcgacaccc gccgggtcga ggtcgcgggg
     1141 gcccgtggcc gctgaccgcc cccgggccgg ccgccgggcc taccgggccc ttgccgcgct
     1201 ggccggcaag gacgtcaact tcgaccccgg ccagagcgag cacttcacca ccgagaacgg
     1261 ctggaagatc gacgactacc tccaggagct gccgcccgag gcgcccggcc cgccggtgcc
     1321 cgggggcagc ttcgaggtcg cccagcggct gatgcgcgac tacgagttcg ccgacccggc
     1381 catcgtgcgg gccgtctacg ccgaggacag ccccttcgag acccgcgaca tgctgctgga
     1441 gggccgcttc tacggcctcc ggttccactt cggcgtccgc gtcggcggtc tggtcgacga
     1501 ggaggtggcc gtcggcggcc gcccggtccg gcgctggggc tggaactacc gcaccctcca
     1561 ggggcacctg gagatggggc agatggacta cgaggtccgc aagtggctcg acagcggcga
     1621 ggtcgacttc cgcatccacg ccttctcccg cccggcccac atccccaacc cggtcatccg
     1681 gctcggcttc cgggtgttcg ggcgctgggt ccagcgccgc ttcgccaggc acgcctgcca
     1741 gcgcatggcc cgcctgaccg ccgcccgggt ggcgcccacg gcccccgcgg gagaccctcc
     1801 ggcgacaagc cgttaacctg gcggttccct ccgaaaccgt tgtgccgcaa cgatttcgga
     1861 cagccgaggg tcgttgcctc cggcgttccc tgggtgtagg ctcctggccc ggaattacat
     1921 ccgtgtcatt tgtccacagg ctgtgggaaa ctccggtcga gcggccttcg caccaacggt
     1981 aggggagagc aacagcagcc atgccgggca gcttcgcgca cctccacgtg cacaccgagt
     2041 actcgatgct cgacggagcc gcccgggtcg acgacctgtt cgccgaggcc gcccgcatgg
     2101 gcatgcccgc cctggccatg accgaccacg gccagctgtt cggcgccgtc gacttctacc
     2161 tggccggcca gaggcacggg gtcaagcccg tcatcgggtg cttgctggct ggccaggaga
     2221 tcatcaccgc cgacggtgtc aagaacgtcg aggatgtccg tgtcggcgac atggtgctca
     2281 cccacaatgg gcggttccgt cgcgtccttc gcaccatgcg ccggccttat gagggacagg
     2341 cgtacaccgt ctcgctcggc ggacggtatg gccggaccct gaccctcacc gaagagcatc
     2401 cgatcctcgt ccgtacgcgt gacggcctgg tggactggac caaaccagcc gacatcgatg
     2461 cgggccaagc gagcaagcat ggaggtgtga actgctggaa gtcctgggcc tgcctgcccc
     2521 ggttgcgcac cgagtggaca gagattcgtt cctccgatct cctaccagac gacttctcgg
     2581 cccccctgac aggcgggatc gctcgcacct acaggt
//
LOCUS       DASSWR010000537         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_811443,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000537 DASSWR010000000
VERSION     DASSWR010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811443"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..552
                     /locus_tag="VFO47_09015"
                     /pseudo
     CDS             116..552
                     /locus_tag="VFO47_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(585..1793)
                     /locus_tag="VFO47_09020"
     CDS             complement(585..1793)
                     /locus_tag="VFO47_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HET8788876.1"
                     /translation="MTEDRMALVELLQKSGEGDFLRAVAEAVLQMLMEADAEGLIGAG
                     RHERSPERLNWRNGYRERTLDTRLGSLQLRIPKLRQGSYFPPFLEPRKMAEKALVAVI
                     QEAWIGGVSTRRVDELVQAMGLAGISKSQVSKLCKEIDERVGGFLDRPLEGEWPYLWL
                     DATYLRVREGGRIVSVAAIIAVAVDTEGRREIVGLGLGPSEAEPFWSTFLKGLVRRGL
                     KGVKLVISDAHEGLKQAAAKVLGATWQRCRVHWMRNALAHVPKGQQTVVAAALRQAFL
                     QADQAGARQVWRRLADQLRPRWPKLAALLDEGEDDVLAYMAFPAQHRAKLHSTNPLER
                     LNKEVKRRADVVGIFPNEPSIVRLIGAVLLEQNDEWQVRHRYMQIEAMAGLASPDPDA
                     ETLGLPPLAA"
BASE COUNT          308 a          815 c          766 g          325 t
ORIGIN      
        1 gtccttggcg ctgccgttcc tggtcttccg cgcggcgccg gtcgccgcca cctgagccgc
       61 ccccacggga gtggtccacc ctgaggtatg gctttggccc ccaaggagga ccgcgatgcc
      121 gcgcaagaga cacacgcccg aggagatcgt cgccaagctg cgccaggtgg acgtcctggc
      181 cgcgcagggc cggagcgtgg ccgacgccgt tcgttcgatc ggcgtcaccg aggtgaccta
      241 ttaccgctgg cggcaggagt tcggtgggct caagaccgag cagatcaggc gttcgaagga
      301 gctggaagcc gagaacgcgc gcctgcgccg ggcggtggcc gacctcacgc tggacaagct
      361 gatcctgaag gaggccgtct cgggaatcac cgagccccgc gcggcgccgc gtccgcatcg
      421 agcacgcccg acgcccgaga caagctccgg gtctccgagc gccgcgtgcg ccgagcgctc
      481 gggcagcacc gctcgacgcg gcgcaaacgc ccgcggggcc gggacgacgg ggttgcgtcc
      541 gtcaacgtgg tggaatttcg atccggctct gaggtggcca ccagtcaagc cgccaggggt
      601 ggaagcccca gggtctcggc gtcgggatcg ggcgaggcca gcccggccat cgcctcgatc
      661 tgcatgtagc ggtgtcggac ctgccactcg tcgttctgct cgagcagcac ggcgccgatc
      721 agcctgacga tcgagggctc gttggggaag atgccgacca cgtcggcccg ccgcttgacc
      781 tctttgttca gccgctccag cgggttcgtg gagtgcagct tggcgcggtg ctgggccggg
      841 aaggccatgt aggccaggac gtcatcctcg ccctcgtcca ggagggcggc gagcttcggc
      901 cagcgcggcc ggagctggtc ggcgagccgc cgccagacct ggcgcgcgcc ggcctggtcc
      961 gcttgcagga aggcctggcg gagggcggcc gcgaccaccg tctgctgccc tttgggcacg
     1021 tgcgccagcg cgttcctcat ccagtgcacc cggcagcgct gccaggtggc gcccagcacc
     1081 ttggcggccg cctgcttgag gccctcgtgg gcgtccgaga tgacgagctt gacgcccttc
     1141 aggcctcggc ggacgagacc cttgaggaag gtcgaccaga acggctccgc ctccgacggg
     1201 ccgaggccca ggccgacgat ctcgcgccgg ccctcggtgt cgaccgcgac ggcgattatc
     1261 gccgcgacgg agacgatccg gccgccctcg cggacccgga ggtacgtcgc atcgagccac
     1321 aggtagggcc attcgccctc gagtggccgg tcgaggaagc cgccgacccg ctcgtcgatc
     1381 tctttgcaga gcttggagac ctggctcttg ctgatgccgg cgaggcccat cgcttgcacc
     1441 agctcgtcca ctcgccgggt cgagaccccg ccgatccagg cctcctggat cacggcgacc
     1501 agcgccttct cggccatctt ccgcggctcg aggaaaggcg ggaagtagga gccctgccgg
     1561 agcttgggga tgcggagctg caaggaccca agccgggtgt cgagcgtccg ctcccggtag
     1621 ccgttgcgcc agttcagccg ctccggcgag cgctcgtggc gccccgcgcc gatcaggccc
     1681 tcggcgtcgg cctccatgag catctgcagc acggcctccg ccaccgcccg caggaagtcg
     1741 ccctcaccgc tcttctggag cagctcgacg agtgccatcc tgtcctcggt catcgggttc
     1801 tccgttcagg tgaagctcgc aactccaccc tagccggcag gcccgatggc cacctcggcc
     1861 gctcaccgaa tttccaccac gaccgtggac gctaccctgg catatgctga cagtccgcgg
     1921 gcggcccgct accctctcgg tcccgccgcc ctcgggagga ccctcccatg gaccgccgca
     1981 ccttgaacat cgcctctgcc gtcgccaccg cggtggctgt cctgatcgtc ctagcctacg
     2041 ccctcggctg gttcggcagc ggcgtagagg ccccggcgcc gcccccggtg ccggccacga
     2101 ccgccccggc tcccgcccca ggggcaggcg ggaccacgca gtaggcgagt cttggcctgg
     2161 cgcgccggcg gcggccggag cggccttgag cgccgccccg gtgacgacga gcgt
//
LOCUS       DASSWR010000538         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_246173,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000538 DASSWR010000000
VERSION     DASSWR010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246173"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1432)
                     /locus_tag="VFO47_09025"
     CDS             complement(110..1432)
                     /locus_tag="VFO47_09025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET8788877.1"
                     /translation="MAEREFKLPDLGEGLTEGEVVRWLVAEGDAITLNQPIVEVETAK
                     AVVEIPAPYAGTVVKLHASEGDTLDVGAPLISVDTGGGPAGAASPAAASAPVAAEPEQ
                     ELQATLVGPGERQQARRRRIGGHARSANGPRTAAQGQDLAPTGPAQLRPRATPPVRRY
                     AKNRGVDLTALVGTGRDGRITREDVDGALGTAEPAAGAAGAVARQPRERSEERIPVRG
                     TRRQIAAAMTASAFSIPHVTEFLTVDATRLMELRERLRALPAAADLKLTPLAVVAKAL
                     CVALRSFPLMNSSWDEDAGDIVVKGWVNLGIATDTPTGLLVPNIKDADTLGILDLSRE
                     LTRLTALARERKAAPTDLTGGTITITNVGGFGVETGTPIINRPECAIVATGLIAPRPW
                     VVDGQLAVRQLMTTSVSFDHRIVDGAYAAQFLAHLRDLLEDPALLSAF"
     gene            complement(1453..>2039)
                     /locus_tag="VFO47_09030"
     CDS             complement(1453..>2039)
                     /locus_tag="VFO47_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET8788878.1"
                     /translation="SPEAYFAHTAGLRVVSPSNPQDAYTMLQQAIADDDPVIFLEPKR
                     RYWAKQEVDLPVATEPIGQARVLRQGSDLTLVAYGPMVKVAMDAAEAAQEEGVGSLEV
                     IDLRSLVPLDEETLVASAARTGRMVVVHEAPQFLGMGAEVAARVTEAAFFHLEAPVLR
                     CTGLDIPYPPARLEEAHLPGVDRVLWTVQRSLDF"
BASE COUNT          239 a          689 c          834 g          277 t
ORIGIN      
        1 ccgggcaggt ccagtaccgg gcgcccggcg ggtagcggtt cccgccacgg tcccgcgccg
       61 ctaggagggg cgaccaccgc gccgggtggg tgcctggggc ccgacgggcc tagaacgcgc
      121 tcagcagggc ggggtcttcg agcaggtcgc ggaggtgggc gaggaactgg gcggcgtagg
      181 cgccgtcgac gatgcggtgg tcgaaggaga ccgaggtcgt catgagctgg cggacggcga
      241 gctggccgtc gaccacccag gggcgggggg cgatcaggcc ggtggcgacg atggcgcact
      301 cgggcctgtt gatgatgggg gtgccggtct cgaccccgaa gccgccgacg ttggtgatgg
      361 tgatggtgcc gccggtgagg tcggtggggg cggccttgcg ctcgcgggcc agggcggtca
      421 ggcgggtgag ctcgcgtgac aggtcgagga tgccgagggt gtcggcgtcc ttgatgtttg
      481 ggaccagcag gccggtcggg gtgtcggtgg cgatgccgag gttgacccag cccttgacga
      541 cgatgtcgcc ggcgtcctcg tcccaggacg agttcatcag cgggaacgaa cggagggcga
      601 cgcacagcgc cttggcgacc acggcgagcg gggtgagctt gaggtcggcc gccgccggga
      661 gggcgcggag gcgctcccgc agctccatca ggcgggtcgc gtcgacggtc aggaactcgg
      721 tgacgtgcgg gatcgagaac gccgaggcgg tcatggcggc ggcgatctgg cgccgggtgc
      781 cgcgaaccgg gatccgctcc tcgctgcgct cgcgcggttg ccgggccacg gccccggcgg
      841 ccccggcggc gggctcggcg gtcccgaggg cgccgtcgac gtcctcgcgg gtgatgcggc
      901 cgtcgcggcc ggtgccgacc agcgccgtca ggtcgacccc gcggttcttg gcgtagcggc
      961 gcaccggcgg ggtcgcccgg gggcgcagct gggccggccc ggtcggggcc aggtcctggc
     1021 cctgggcggc ggtacgggga ccgttggcgc tgcgggcgtg gccgccgatg cggcggcggc
     1081 gggcctgctg gcgctcccct ggcccgacca gggtggcctg gagctcctgc tcgggctcgg
     1141 ccgccaccgg ggccgaggcg gccgccgggg acgcggcccc ggccggcccc ccgccggtgt
     1201 cgacgctgat caggggggcg cccacgtcca gggtgtcgcc ctcgctggcg tggagcttga
     1261 ccactgtccc ggcgtacggg gccgggatct cgaccaccgc cttggccgtc tccacctcga
     1321 cgatcggctg gttgagggtg atggcgtcgc cctcggcgac cagccagcgc accacctcgc
     1381 cctcggtgag cccctcgccc aggtcgggga gcttgaactc gcgctcggcc atcgggacct
     1441 tctcctcctg agctagaagt ccaggctccg ctggacggtc cacaggaccc ggtcgacccc
     1501 ggggaggtgg gcctcctcga gccgggccgg cgggtacggg atgtcgagcc cggtgcagcg
     1561 cagcacgggc gcctccaggt ggaagaaggc ggcctcggtg acacgggccg cgacctcggc
     1621 gcccatgccg aggaactgcg gggcctcgtg caccaccacc atccggccgg tccgggcggc
     1681 ggaggcgacc agcgtctcct cgtccagcgg caccaggctg cgcaggtcga tgacctcgag
     1741 cgagccgacg ccctcctcct gggcggcctc ggccgcgtcc atggcgacct tgaccatggg
     1801 gccgtaggcg accagggtca ggtcgctgcc ctggcgcagc acgcgggcct ggccgatcgg
     1861 ctcggtggcg accggcaggt cgacctcctg cttggcccag tagcggcgct tgggctccag
     1921 gaagatcacc gggtcgtcgt ccgcgatggc ctgctgcaac atcgtgtagg cgtcctgcgg
     1981 gtttgacggg gagaccacgc gcaacccggc ggtgtgggcg aagtaggcct cgggcgatt
//
LOCUS       DASSWR010000539         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_18303,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000539 DASSWR010000000
VERSION     DASSWR010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18303"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1057
                     /locus_tag="VFO47_09035"
     CDS             170..1057
                     /locus_tag="VFO47_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8788879.1"
                     /translation="MTHLRTIRRPRLRWIVTAALLASLAGLVLSQVGAADAAGSTTAG
                     AATAGAATSHGGSKPTVVLVHGAWADSGSWDQVVARLQRQGYPVIAFPTPLRSLPDDS
                     AYLAAFLSSVPGPIVLVGHSYGGAVITNAATGIANVKALVYVDGFLPDEGETLGELLG
                     TPPGSCVLGPPTEIFDLRPIPGAPPEVVDAYVKQSLFPSCFANDLPAKQAAVLAATQR
                     PGSTLTLGQPSGPPAWAEIPSWALVGTVDRVILPATQLFMAERAGARIVKVKASHLSM
                     ISQPGAVTRLILAAARSTA"
     gene            1207..2472
                     /locus_tag="VFO47_09040"
     CDS             1207..2472
                     /locus_tag="VFO47_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HET8788880.1"
                     /translation="MLSVVTDAQAHDELRLDLDAIVREGARRMLAAALEAEVDDYLAA
                     HAVERDEGGRRLVVRNGHARQREVTTAAGSIPVRAPRVDDRRTDLVSGGRVRFRSVIL
                     PPWCRKSPKVAEVLPLLYLHGLSTGDFVPALEAFFGSSAGLSAAAVGRLLASWQADYQ
                     VFCRRDLADRDYVYVWADGVHFRVRLEQARLCCLVIVGVRADGTKELVAVADGEREST
                     DSWAELLRDLRRRGMQAPVVAVGDGALGLWAALRDVFPATRHQRDWVHKLANVLGCLP
                     ASVQAGARTALAEIRDAPDRDHAQRAIEAFARDYGTKWPKAVAKITDDAEELLCFFDF
                     PAEHWLHLKTSNPIESTFSTVRLRTRVTKGPGSKAAGLAMAFKLLEAAQDRWRAVNGP
                     HLVALVRAGARFDKGVMIKRHNEDEEVAA"
BASE COUNT          388 a          953 c          914 g          405 t
ORIGIN      
        1 tcgtgatcaa cggcccgagt cgatatcttc ctcggtcctt acttcgcagg caccgggtcg
       61 ccagccactg gtacgacctc catacgccag caccgacccc gatcgtgtga cgaacgatgg
      121 agcacctccg gatgagcaac cccggcagcc gggaatggaa ggaagtgcca tgacccacct
      181 ccgaacgatc cgccggccac ggctgcgctg gatcgtgacc gccgcgctcc tggccagcct
      241 ggccggtctg gtcctgagcc aggtcggggc cgccgacgcc gccggctcta ccaccgccgg
      301 cgcagccacg gctggtgcag caacctcaca tggagggtcc aagccgaccg tggtgctggt
      361 gcacggcgcc tgggccgaca gcggcagctg ggaccaggtg gtggcccgcc tgcaacgcca
      421 gggctacccc gtcatcgcct tccccacccc gctgcgctcg ctccccgacg acagcgcgta
      481 tctggccgcc ttcctgagca gcgtccccgg ccccatcgtc ctggtcggcc actcctacgg
      541 cggggcggtc atcaccaacg ccgccaccgg cattgccaac gtcaaggcgc tggtctatgt
      601 cgacggcttc ctccccgacg agggcgagac cctcggcgag ctcctcggga ccccgccggg
      661 ctcctgtgtg ctcggtcccc ccaccgagat cttcgacctg cggcccatcc ccggcgcccc
      721 gccagaggtc gtggacgcct atgtcaagca gagcctgttc ccgtcctgtt tcgccaacga
      781 cctgcccgcc aagcaggcgg ccgtcctggc cgccacccag cggcccggct cgaccctcac
      841 cctcggccag ccgtccgggc caccggcctg ggccgagatt ccgtcctggg cgctggtcgg
      901 cacggttgac cgggtgatcc tgccggcgac ccagctgttc atggccgagc gggccggtgc
      961 ccgcattgtc aaggtcaagg cctcgcacct gtcgatgatc tcccaacccg gcgcggtgac
     1021 ccgcctgatc ctcgccgcgg cccgcagcac cgcctgagcc agggctgttg gatcaccggc
     1081 cggtgtcccg cgaggtatga cctgaagttg tggatcgggt ctggagatgg gagcgcggcg
     1141 taccttcccg gatgacaagt cctggaagcg ccggcgcggt gaacgccggc ggggaaggaa
     1201 cgccgcatgc tgagtgtagt caccgatgcc caggcgcatg acgagctgcg cctggacctg
     1261 gatgcgatcg tgcgcgaggg tgctcggcgg atgctggccg ccgccttgga ggccgaggtc
     1321 gacgactacc tggccgcgca cgcggtcgag cgggacgagg gtggccggcg gctggtggtg
     1381 cgcaacggcc acgcccgcca gcgtgaggtc acgaccgcag ccgggagcat cccggtgcgt
     1441 gccccgcggg tggacgaccg ccgcaccgac ctggtgagcg gtggtcgggt ccggttccgc
     1501 tcggtgatcc tgccgccctg gtgccgcaag agccccaagg tcgctgaggt actgccgctg
     1561 ctgtacctgc acggcctgtc cacgggggac ttcgtgcccg ccctggaggc cttcttcggc
     1621 tcatcagctg ggctgtcggc ggcggccgtc ggtcggctgc tggccagctg gcaggccgac
     1681 taccaggtgt tctgccgccg cgacctggcc gaccgcgact acgtctatgt ctgggccgac
     1741 ggtgtgcatt tccgggtgcg gctggagcag gcccgcctgt gctgcctggt gatcgtgggc
     1801 gtgcgcgccg acggcaccaa ggagctggtg gccgtggccg acggcgagcg tgagtccacc
     1861 gacagctggg cggagctgct gcgggacctt cggcggcgcg gtatgcaggc accagtcgtc
     1921 gccgtgggcg acggcgcgct tgggttgtgg gcggcgctcc gcgacgtctt cccggccacc
     1981 cgccaccagc gcgactgggt ccacaagctc gccaacgtgc tgggatgcct gcccgccagc
     2041 gtgcaggctg gcgcgcgcac ggcgttggct gagatccggg atgcgcccga ccgtgaccac
     2101 gcccagcggg cgatcgaggc gttcgctcga gactacggca ccaagtggcc caaggcagtg
     2161 gccaagatca ctgacgatgc cgaggaactg ctctgcttct tcgacttccc ggccgagcat
     2221 tggctgcacc tcaagaccag caacccgatc gagtcgacct tctccaccgt ccggctcaga
     2281 acccgggtga ccaagggtcc cggctccaag gccgcgggcc tggcgatggc gttcaagctg
     2341 ctggaggccg cccaggaccg ctggcgggcg gtcaacggcc cccatctggt cgcgctggta
     2401 cgcgccggcg cccgcttcga caagggggtg atgatcaagc gtcacaacga ggatgaagag
     2461 gtcgccgcgt aatcatccca gacccggcca tccacaactc ttgaccattc ctcggtcgcc
     2521 cggatcgggc ggcacaagca gataggcatg ccgttcaggc tcgggagttg tacgagctgg
     2581 ccctggtgga gcagacggag caggcacagc aggtcctgtc gccacggggt agcactcacc
     2641 gggggagcaa cgcactcggt
//
LOCUS       DASSWR010000540         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_471476,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000540 DASSWR010000000
VERSION     DASSWR010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471476"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /gene="murC"
                     /locus_tag="VFO47_09045"
     CDS             complement(<1..1190)
                     /gene="murC"
                     /locus_tag="VFO47_09045"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET8788881.1"
                     /translation="MSGPLPVGGTVHLVGVGGAGMSGLARLLLAAGHRVTGSDRSESA
                     TLAALRALGAEVWAGHDGARLGRPDLVVASTAIRPTNPELAAARILDIPVLGRAQLLA
                     RLMAGRTGIAVAGTHGKTTTTGMVVAILEAAGLDPSFAVGGDFKASGVNAAAGSGPHF
                     VAEADESDGSFLELAPAMAVVSNVEADHLDHWGDLDAVRGAFRAFVGRLPADGTAVLC
                     ADDRGALELAGAAPCPVVTYGFAAGADLRGEVLAIDGRGARFAAVAADQPLGEVALVV
                     PGRHNVANALGAIAASRAAGAPFEAAVAGLAGFTGAARRFHLRAEAGGVTVVDDYAHH
                     PTEVAASLAAARLGGWKRVVAVFQPHLYSRTRLFADEFGRALAAADLVVVADVYAARE
                     DPEPG"
     gene            complement(1187..>2625)
                     /gene="murG"
                     /locus_tag="VFO47_09050"
     CDS             complement(1187..>2625)
                     /gene="murG"
                     /locus_tag="VFO47_09050"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET8788882.1"
                     /translation="PAPGPGGLPAPGDRPGLSLLVAAGGSGGHVFPGLALARTVVRHE
                     PEATVRFAGTTRGIETRAVPEAGFALDLLPILPLSRRLAKETLLAPFAAVNGTFAARR
                     LLREHHFDVVCGMGGYVTLPVAVAARLEGVPVVLHEQNAVPGVANRLAARVASRIAVG
                     VAAAAEAFPPERTTVVGNPVRPELARLDRAALHDEAVADFGLDPGRRTLFVFGGSQGA
                     RHINQAVIAATAHWPDPGAVQVLHACGRRDEADVRAAWAAADPDGRGLLVKIVPFVDR
                     MDLAYAAADLAMTRAGAITVAELTAAGMPAVMVPLPHATADHQAANAWAVAAGGGAVV
                     VDDAALDGPAVAAAAGPLLADPERLAEMAAAMRAQAHPAAAEELAALVVEASGRRTRE
                     EFLADVAAAATPVDREATGWFESAAAPAGGPSVQRRVDHRSKRMEAYPPVDRKAHPPR
                     EDPPAGDASTNGRDARAARPPTDRDAGP"
BASE COUNT          292 a         1005 c         1066 g          262 t
ORIGIN      
        1 ccgggttcgg gatcctccct ggccgcgtag acgtcggcca cgaccaccag gtcggcggcg
       61 gcgagggccc ggccgaactc gtcggcgaac agccgggtgc gcgagtacag gtggggctgg
      121 aacacggcca ccacccgctt ccagccgccc aggcgggccg cggccaggct ggccgcgacc
      181 tcggtgggat ggtgggcgta gtcgtcgacc accgtgaccc ccccggcctc ggcccgcagg
      241 tggaaacgcc gggccgcgcc cgtgaacccg gccaggccgg ccacggcggc ctcgaacggc
      301 gccccggcgg cccgggaggc cgcgatggcc cccagcgcgt tggccacgtt gtggcggccg
      361 gggacgacca gggcgacctc gcccagcggc tggtcggcgg cgaccgcggc gaagcgggcg
      421 ccccggccgt cgatggccag cacctcgccc cgcaggtcgg ccccggcggc gaagccgtag
      481 gtgaccaccg ggcagggggc ggccccggcc agctcgaggg cgccccggtc gtcggcgcag
      541 agcacggccg tgccgtcggc gggcagccgg ccgacgaagg cccggaaggc gccccggacg
      601 gcgtccaggt cgccccagtg gtcgaggtgg tcggcctcga cgttggagac cacggccatg
      661 gccggggcca gctccaggaa cgagccgtcg gactcgtcgg cctcggccac gaagtggggg
      721 ccgctcccgg cggcggcgtt gacgccgctg gccttgaagt cgccgccgac ggcgaacgac
      781 gggtcaagcc cggccgcctc caggatggcc accaccatcc cggtggtggt cgtcttgccg
      841 tgggtgccgg cgacggcgat gccggtcctg ccggccatga gcctggccag gagctgggcc
      901 cggccgagga cggggatgtc gaggatcctg gccgcggcca gctccgggtt ggtcggccgg
      961 atggcggtcg aggcgaccac caggtcgggc cggcccagcc gggcgccgtc gtggccggcc
     1021 cacacctcgg cgccgagcgc ccgcagcgcc gcaagggtgg ccgactcgct gcggtccgac
     1081 ccggtgaccc ggtggcccgc ggccagcagc aggcgcgcca gcccgctcat cccggccccg
     1141 cccaccccca ccaggtgcac ggtccccccg accgggagcg ggccgctcat ggcccggcgt
     1201 cccggtcggt gggtggccgg gcggcccggg cgtcgcggcc gttcgtggag gcgtccccgg
     1261 ccggggggtc ctctcgaggg gggtgcgcct tgcggtcgac cggcgggtag gcctccatgc
     1321 gcttgctgcg gtggtcgacc cggcgctgca ccgacgggcc gccggcgggg gcggcggccg
     1381 actcgaacca gccggtggcc tcccggtcga ccggggtcgc ggccgcggcc acgtcggcca
     1441 ggaactcctc ccgggtgcgc cggcccgagg cctcgacgac cagggcggcc agctcctcgg
     1501 cggcggccgg gtgggcctgg gcgcgcatcg cggccgccat ctcggccagg cgctcggggt
     1561 cggccagcag cggcccggcg gcggcggcca ccgccgggcc gtcaagggcg gcgtcgtcga
     1621 ccaccacggc cccgccgccg gcggcgaccg cccaggcgtt ggccgcctgg tggtcggcgg
     1681 tggcgtgggg cagcgggacc atcacggccg gcatcccggc ggccgtcagc tcggccacgg
     1741 tgatggcgcc ggccctggtc atggccaggt cggcggcggc gtaggccagg tccatgcggt
     1801 cgacgaacgg gacgatcttg accagcagcc cccggccgtc ggggtcggcc gcggcccagg
     1861 cggcccggac gtcggcctcg tcccgccgcc cgcaggcgtg caggacctgg acggcgccgg
     1921 ggtcgggcca gtgggcggtg gccgcgatca ccgcctggtt gatgtggcgc gccccctggc
     1981 tgccgccgaa cacgaacagg gtgcgccggc cggggtcgag gccgaagtcg gccaccgcct
     2041 cgtcgtgcag ggccgcccgg tcgaggcggg ccagctcggg ccggaccggg ttgccgacca
     2101 ccgtggtccg ctcgggcggg aaggcctcgg ccgccgccgc caccccgacc gcgatccggc
     2161 tggccacccg ggcggccagg cggttggcga ccccgggcac ggcgttctgc tcgtggagca
     2221 cgaccggcac gccctcgagc ctggccgcca ccgccaccgg gagggtgacg tagccgccca
     2281 tgccgcagac cacgtcgaag tggtgctcgc ggagcaaccg gcgggcggcg aaggtgccgt
     2341 tgacggcggc gaacggggcc agcagggtct ctttggccag ccggcgcgac aggggcagga
     2401 tcggcagcag gtcgagggcg aagccggcct cgggcaccgc ccgggtctcg atccccctgg
     2461 tcgtgccggc gaagcggacg gtcgcctcgg gctcgtggcg gacgacggtg cgggccagcg
     2521 ccagcccggg gaacacgtgc ccgcccgacc cgccggccgc gaccagcagc gacaggccgg
     2581 gccggtcccc gggcgccggc aggccccctg gccccggagc cggag
//
LOCUS       DASSWR010000541         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_387638,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000541 DASSWR010000000
VERSION     DASSWR010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387638"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..847
                     /locus_tag="VFO47_09055"
     CDS             188..847
                     /locus_tag="VFO47_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET8788883.1"
                     /translation="MSVYQQDTAGQGNGEGPPPFRMVRRGYDRDEVDAYIQQLRARLE
                     EAVDLYAKTEGARAELERETRNLREGSPSFEQLGGEAAALLQEAGRSGEQLIENARRR
                     ADTIIEKAQRQAEQTRADVESEAQKALEQAREVADHIRREVEEERAALFSETEQVREF
                     RDSLLDDLGRVHGEISGLLERTRKQREGALTIGGLAPPSQVADPKPETPAEPAKVSER
                     Q"
     gene            complement(869..1216)
                     /locus_tag="VFO47_09060"
     CDS             complement(869..1216)
                     /locus_tag="VFO47_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788884.1"
                     /translation="MLGGRRLPMRQFFTVTGLILIVFAAGLVSRTILWLQAAGELGTV
                     WNNVYDLTAYRWLTVSTESGRFLGAMFGWDPRPSIEQVAAYLLFLVTVAGLFLRTPRA
                     AQAQAAAPGHGSR"
     gene            1170..1754
                     /locus_tag="VFO47_09065"
     CDS             1170..1754
                     /locus_tag="VFO47_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788885.1"
                     /translation="MTVKNWRMGRRRPPSTTRAPRTDAAARRKAAGQDGPAVLGGGGG
                     DQEQGRLQALAGHGQEGHRHDGQAGVAVAGDGRVDAALELALDAVGLAPHPEDHPGED
                     GHGQQRHGREGEQPGLAPEVLDGEVDDDPGGDGQGGGRGHALPHGPDQVAAADPPDVG
                     VDDGHDQRGLEALAEHDQQRGQHRPGPPATRERS"
     gene            complement(1761..>2005)
                     /locus_tag="VFO47_09070"
     CDS             complement(1761..>2005)
                     /locus_tag="VFO47_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788886.1"
                     /translation="HPSVPGPVVFAHTSPVWVEVGGRPVRRPASARWLLDWLDRFEAL
                     LDEHGRFAGDDQRADLATVVDQARSWYRAIAGDPVC"
BASE COUNT          316 a          687 c          798 g          204 t
ORIGIN      
        1 ctccggtcgc gggacgggtg agcaggatgg tggtgtcctc caggctgtct ggagcaggag
       61 gtcccgcgct gagaaggtcc cgcgtggttg gctgggtcgc tggggtttgc acctcggggg
      121 cggaggtggg atcttcctgc atcggcgggc cttgcgacgg gttcggcgcg ccgtgacgga
      181 ggttgccatg agcgtgtacc agcaggacac agccgggcag ggcaacgggg aggggccgcc
      241 gccgttccgg atggtccggc gcgggtacga ccgcgacgag gtggacgcct acatccagca
      301 gctcagggcg cgcctcgagg aggcggtcga cctctacgcc aagaccgaag gggcgcgggc
      361 cgagctggag cgggagaccc ggaacctccg ggagggctcg ccgtcgttcg agcagctcgg
      421 cggggaggcg gccgcgctgc tccaggaggc gggccgttcg ggcgagcagc tgatcgagaa
      481 cgcccggcgg cgcgccgaca cgatcatcga gaaggcccag cggcaggccg agcagacccg
      541 ggccgacgtc gagagcgagg cccagaaggc gctggagcag gcccgcgagg tcgccgacca
      601 catccgccgg gaggtcgagg aggagcgggc ggcgctgttc agcgagaccg agcaggtgcg
      661 cgagttccgc gacagcctgc tggacgacct cggccgggtc cacggcgaga tcagcggcct
      721 cctggagcgc acccgcaagc agcgcgaggg cgccctgacc atcggcggcc tcgctccccc
      781 gtcgcaggtg gccgacccca agccggagac cccggccgag cccgccaagg tctccgagcg
      841 ccagtagcgc acggcgcccc gccgccgctc aacgcgaccc gtgacccggg gccgcggcct
      901 gggcctgggc ggcccgcggg gtccgcagga acagcccggc caccgtcacc aggaacagca
      961 ggtaggcggc gacctgctcg atcgaggggc gagggtccca gccgaacatg gccccgagga
     1021 agcggccgga ctcggtgctg acggtcagcc agcggtaggc ggtcaggtcg tagacgttgt
     1081 tccagacggt gccgagctcg ccggcggcct ggagccagag gatcgtgcgc gagaccaggc
     1141 cggcggcgaa cacgatcagg atcaggccgg tgacggtgaa gaactggcgc atgggcaggc
     1201 gccggccgcc gagcacgacc agggcgccga ggaccgacgc cgccgccagg cgcaaggccg
     1261 ccggccagga cggcccagcc gtcctcggtg gtggcggcgg cgatcaggaa cagggccgcc
     1321 tccaggccct cgcgggccac ggccaggaag gccaccgtca tgacggccag gcgggcgtcg
     1381 ccgttgccgg cgatggccgc gtcgacgctg ccctcgagct cgcccttgat gcggtgggcc
     1441 tggcgccgca tccagaagat catccaggtg aggacggcca cggccagcag cgacacggcc
     1501 gcgaaggcga gcagccgggg ctcgccccgg aggttctcga cggtgaggtg gacgacgatc
     1561 ccggcggcga cggacagggc ggcggccgcg gccatgccct gccacatggg cccgatcagg
     1621 tcgcggcggc cgatccgccg gatgtaggcg tagacgatgg ccacgaccag cgcggcctcg
     1681 aagccctcgc ggagcatgat cagcagcgcg gtcagcacag gcccggccct ccagcgacgc
     1741 gggaaaggag ttaggccagc ctaacagacg gggtcgccgg cgatcgcccg gtaccaggag
     1801 cgggcctggt cgaccacggt ggcgaggtcg gcccgctggt cgtcgccggc gaagcggccg
     1861 tgctcgtcca gcagcgcctc gaagcggtcc agccagtcaa gcagccagcg ggcgctggcc
     1921 gggcggcgga ccggccggcc gccgacctcg acccagacgg ggctggtgtg ggcgaacacc
     1981 accggacccg gcaccgacgg gtggc
//
LOCUS       DASSWR010000542         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_77336,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000542 DASSWR010000000
VERSION     DASSWR010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77336"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFO47_09075"
     CDS             complement(<1..926)
                     /locus_tag="VFO47_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET8788887.1"
                     /translation="MRAVTISDGRLVVAERPDPEPGRGELLVRVAAAGLNGADLQQAR
                     GRYPAPPGSPADIPGLELAGEVAAIGPDVFRFEPGDRVMAVVGGGGQAELAVIHERVA
                     IPVPDGLGWPEAGGFPEVVTTAHDALFTQCGLGLGDRLLVHGAAGGVGTAAVQLAAAA
                     GAEVVASVRNPGHHQAVADLGAAVVAEPAQAFGHGPFDVILELVGAPNVPADVDSLAT
                     GGRLMVIGFGAGATAEIDLRKLWARQARVLASNLRLRPLELKADAARRVEHQTLPLVA
                     GGRLRIPVAATFPLDAAQEAYDRFAAGGKLGKL"
     gene            complement(936..1571)
                     /locus_tag="VFO47_09080"
     CDS             complement(936..1571)
                     /locus_tag="VFO47_09080"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788888.1"
                     /translation="MVGSVQVEPGRRRRRRRQDSEQEILEAAGRLLRERPFRELTVDD
                     LMAATSQSRTAFYRHFTDRQDLLVHLIRDLNEELWEMSAGWLRGRGDPLAEGRESLER
                     LADVYAAHGPLLRAIAEAASHDADVEAVYRGLVQGFVDATAARIRRDVAAGRTSLAHP
                     DHVAAALVWMTERHLAATFGRVGATDADRSAALETLFTIWMRTLYSPDPQT"
     gene            1654..>2394
                     /locus_tag="VFO47_09085"
     CDS             1654..>2394
                     /locus_tag="VFO47_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788889.1"
                     /translation="MPPIPAADAPQLFVLDEPAAEEAAAREARQRPGPALEGRRRQGR
                     GSRRPVPVLLAVDGNGLAHRAFHAVGGVEGPAGEARQRVLAMLARIGAATRPSACVVG
                     FDDPIRSLRRDRHPGYKSARPPKDDALVDLLGELPGLLSELGICVVVPAGLEADDVLG
                     SAAALARRRGVAAALATGDQDAFALVAPTVQVFYLVTGGRMEQVSPSWLRARYGVGPA
                     AYPALAALRGDASDCLPGVKGIGAVTAAR"
BASE COUNT          270 a          892 c          959 g          273 t
ORIGIN      
        1 agcttgccca gcttgccgcc ggcggcgaag cggtcgtaag cctcctgggc ggcgtccagg
       61 gggaaggtgg cggccaccgg gatgcgcagc cggccgccgg cgaccagcgg cagggtctgg
      121 tgctcgaccc ggcgagcggc gtcggccttc agctccagcg ggcgcaagcg caggttggag
      181 gcgagcacgc gggcctggcg ggcccacagc ttgcgcaggt cgatctcggc ggtggcgccg
      241 gcgccgaacc cgatgaccat gaggcggccg ccggtggcca gcgagtccac gtcggcgggg
      301 acgttggggg cgccgaccag ctccaggatg acgtcgaagg ggccgtggcc gaacgcctgg
      361 gcgggctcgg cgaccacggc ggcgccgagg tcggccacgg cctggtggtg gccggggttg
      421 cggacgctgg ccaccacctc ggccccggcg gcggcggcca gctggacggc ggccgtgccg
      481 accccgccgg cggcgccgtg gaccagcagc cggtcgccga ggccgaggcc gcactgggtg
      541 aagagggcgt cgtgggcggt ggtgacgacc tcggggaagc cgccggcctc gggccagccg
      601 agcccgtcgg ggacggggat ggccacccgc tcgtggatca cggccagctc ggcctggccg
      661 cccccgccga ccaccgccat cacccggtcg ccgggctcga agcggaagac gtcggggccg
      721 atcgcggcaa cctcgccggc cagctccagc ccggggatgt cggccgggct gcccggcggg
      781 gccgggtagc ggcccctggc ctgctgcagg tcggcgccgt tgagcccggc ggccgcgacc
      841 cggaccagca gctcgccccg gcccggctcc gggtcggggc gctcggccac caccagccgc
      901 ccgtcggaga tcgtgaccgc ccgcatgcgg cgagcctacg tttggggatc cgggctgtac
      961 agcgtccgca tccagatggt gaacagcgtc tccagcgcgg ccgagcggtc ggcgtcggtc
     1021 gccccgaccc ggccgaaggt ggcggccagg tggcgctcgg tcatccagac cagggcggcg
     1081 gccacgtggt cgggatgggc caggctggtc cgaccggcgg ccacgtcgcg gcggatgcgg
     1141 gccgcggtgg cgtccacgaa cccctggacc aggccgcggt agacggcctc gacgtcggcg
     1201 tcgtggctgg ccgcctcggc gatggcccgc agcaggggac cgtgggcggc gtacacgtcg
     1261 gccaggcgct ccagcgactc gcggccctcg gccagcgggt cgccgcggcc ccggagccag
     1321 ccggcgctca tctcccacag ctcctcgttg aggtcgcgga tgagatggac gagcaggtcc
     1381 tggcggtcgg tgaagtggcg gtagaaggcg gtgcgggact ggctggtggc ggccatcagg
     1441 tcgtcgacgg tcagctcccg gaacggccgc tcgcgcagca gccgcccggc cgcctccagg
     1501 atctcctgct ccgagtcctg gcggcgacgc cggcgacgcc ggcccggctc cacctggacg
     1561 ctgcccacca tcgccacaat ctaccaaggc cggccgccag gaccgaagaa ccggcacctt
     1621 gcgcgcttgg cgtccgaggg tgatcgttgt cgcatgcctc ccatacccgc cgccgacgcc
     1681 ccccagctgt tcgtgctcga cgagccggcg gccgaggagg cggcggcccg ggaggcgcgg
     1741 cagcggccag ggcccgcgct cgaggggcgg cggcggcagg gccgcgggtc gcggcggccg
     1801 gtgccggtgc tgctggccgt cgacggcaac gggctggccc accgggcctt ccacgccgtc
     1861 ggcggggtcg aggggccggc gggggaggcg cgccagcggg tcctggccat gctggcccgc
     1921 atcggggccg cgacccgtcc cagcgcctgc gtggtcggct tcgacgaccc gatccgctcg
     1981 ctgcggcgcg accgccatcc cggctacaag tcggcccgcc cgcccaagga cgacgccctg
     2041 gtcgacctgc tcggcgagct gcccgggctg ctgtcggagc tcggcatctg cgtggtcgtc
     2101 ccggcggggc tggaggccga cgacgtgctc ggctcggccg ccgccctggc ccggcggcgc
     2161 ggagtggcgg ccgccctggc caccggcgac caggacgcgt tcgcgctcgt cgcccccacc
     2221 gtccaggtct tctacctggt cacgggcggg cgcatggagc aggtctcgcc gagctggctg
     2281 cgggcccgct acggcgtcgg gccggccgcc tacccggcgt tggcggccct gcggggcgac
     2341 gccagcgact gcctgcccgg ggtcaagggc atcggcgcgg tgaccgccgc ccgg
//
LOCUS       DASSWR010000543         8451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1319993, whole genome shotgun sequence.
ACCESSION   DASSWR010000543 DASSWR010000000
VERSION     DASSWR010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319993"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..617)
                     /locus_tag="VFO47_09090"
     CDS             complement(60..617)
                     /locus_tag="VFO47_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET8788890.1"
                     /translation="MALEIEFAGRRPVVDAAAFVAHTAVLVGDVTVAAGASVWYGAVL
                     RGDFGPIVVGAGSNVQDNCVLHAAEGLPTVLEDEVTVGHLATMEGCVIERGALIGMGA
                     VVLQRARVGRGAVIAAGSVVAEDAQIPPGVLAAGVPAQVKRELSGRASQWGRTAAGEY
                     QRLVPRYLAEARVIRPARSGRATGR"
     gene            complement(624..1718)
                     /locus_tag="VFO47_09095"
     CDS             complement(624..1718)
                     /locus_tag="VFO47_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8788891.1"
                     /translation="MTAEPDATVESSDGAPLGVSAPPPRSLPRAFLPIAVTGALLALV
                     PLWLGDSRLMMGLAVDALVFGAYAVAFNVIFGSTNQLFLCVGALAGVGGYTAAILSEE
                     AGLPLAFGLVAATVLATAVGGLLSWVAVRRSLGVIFTGIVTLAFSLSFANLVLGQREL
                     TGGETGLVVAAGEGTFLQERVAAYYLFLGMLVGFLVVFRALERSHVGWAFRALRDNEL
                     AAELTGVDVARYRVYAGLIGSAMLGITGAVFTYSDGFISPSTFEFGHVDVRVLVMLAF
                     GGLGTLLGPVLGTVVFAVVDELLVDAGQLRTVLYGLLIIGLFLGFKRGAVPAVADFLR
                     VLVRQARSSAGGRRVRAPARRRPDHPDEEG"
     gene            complement(1721..2572)
                     /locus_tag="VFO47_09100"
     CDS             complement(1721..2572)
                     /locus_tag="VFO47_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8788892.1"
                     /translation="MRAVVLDGIVLGLQFGLLGVGMTLVYGLGGVLNLSYGQAAVLAA
                     MVVALSLAAGMPALVAAGAGLLVAAAGGVVLDRTLMQPVYRQRGEHRVLLGLLLTLGV
                     AFVIDGLLTWRYPSGALRLEVGGEAVPILGVRMRVGSLWASGISLAVGAALLLFFRRT
                     TLGRATRSVIQDEVGARLVGINPAAVRTLIFALSGALAGLVAETRSMTSPVAVTAGFD
                     FTILALIVAVVGGLGSVAGAFLAGLLLGIVSTVSAFYIGQYVTTIVLLAAAALTIVVR
                     PRGLLGR"
     gene            complement(2569..3267)
                     /locus_tag="VFO47_09105"
     CDS             complement(2569..3267)
                     /locus_tag="VFO47_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788893.1"
                     /translation="MLEASGLEAGYLGENVVEGVDLRVAAGEAVAVIGSNGAGKTTLF
                     RAICGMLPVSAGRVRFDGRDITGWPAHRVARAGLAYVPAERHLFPGMSVRENLLLGAY
                     PGRPDPSVFELVFDLFPRLKERPRQPAGSLSGGEQQMLAVGRALMSRPRLLMLDEPTT
                     GLAPKLARSAYQALQALKETGVTLLVAEQQVPLALSLADRGYVLENGRVRLEGASEDL
                     ERNPEVRRAYLGVA"
     gene            complement(3260..3997)
                     /locus_tag="VFO47_09110"
     CDS             complement(3260..3997)
                     /locus_tag="VFO47_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8788894.1"
                     /translation="MALLAVDAVEKRFGGLPAVDGVTFGVDEGEILAVVGPNGAGKST
                     LLKTIGGLERPSAGTVTLAGERVDGLAPHRVRQRGIAMVMQTPLTFGSMTVLENVALG
                     AMFGGGDGVVAEPEAIRRAQEALAFVGLAGRADQPVASLNLHQQRFLEMAKALAGRPR
                     LLLVDEVMAGLNDAELAASVQIVRTARDTLGITIIWVEHVMQAVMRLAERVIVLNFGR
                     MLAEGEPEAVMRHPEVIDAYLGEGHDA"
     gene            complement(4005..5231)
                     /locus_tag="VFO47_09115"
     CDS             complement(4005..5231)
                     /locus_tag="VFO47_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8788895.1"
                     /translation="MRHRSRVGLFSLWLVAALLLAACGDGDEGGGQEGGGEPIRIGAL
                     TSLTGNFAPWGVQVRDGMQLAVNEINQRGGVDGRQLELVVADDQSKAEEGTRALERLI
                     ERDGVVAVGGIISSDVGLATARIAEQSKVPLFLVKAGAGGILNANSRHTFRTCLPAAP
                     MVAGPILEYVQSKNLTRVGAIVADYAWGQAIKGALEDAFAAAPDIKLQIEVAPVPEQD
                     FTTYLRSLERLDPQMIVATGHPPGAGAITVQSADLGFDVPVTGAYTPRSLVMSGAKGA
                     ALDRYADFGCADVQSNEYQDLARRFLAFSQNNKFFEDDALAGYGIVTTVAQAVGAAGD
                     DPAAIASHLHENTFDIPGYSFELRWTEWGELADARIAFDLIGRGPAPEGVNEAGAWYP
                     ERLLVSEPLEPFRPPS"
     gene            complement(5585..6355)
                     /locus_tag="VFO47_09120"
     CDS             complement(5585..6355)
                     /locus_tag="VFO47_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET8788896.1"
                     /translation="MGELGAGNATAWVTDDDEALAALVNLLVVLADNKYWLGRHLSEW
                     AVGAPSLEVAVASAAIAQGELGQARVLYPLLDELPFPGPVDPTERERAYQVSLLDRPF
                     PTWPHAVSALLLVDSAVTTMLEAMIGGPYEALARRVPRMLEEEAFHWDFAEGRVRELA
                     RLPGGLPQLQARVDESLEELLCWFGPPGEQGVERLRGHGLLGRGNTELRQAWLDRVGP
                     LLEEVGVRLRPERSEDSRLPWERWNRLQRRLDPEPATA"
     gene            complement(6348..6566)
                     /locus_tag="VFO47_09125"
     CDS             complement(6348..6566)
                     /locus_tag="VFO47_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788897.1"
                     /translation="MERYRLFGRTSYPEPLEFQETLTAADDDAAARAALERHGPRWVE
                     LVLIPERAIHWILGPQAPVPSPEGSTDG"
     gene            complement(6576..6779)
                     /locus_tag="VFO47_09130"
     CDS             complement(6576..6779)
                     /locus_tag="VFO47_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788898.1"
                     /translation="MGDDPRRQVWEVYARHDREERAHHVGTVRAVGPKDARVFAFMMY
                     DERRWQEMFVVPRAAVVPVVAPE"
     gene            complement(6784..7089)
                     /locus_tag="VFO47_09135"
     CDS             complement(6784..7089)
                     /locus_tag="VFO47_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET8788899.1"
                     /translation="MVSVDDVRAALAEVEDPELPVGIVDLGLVRSVVVDGTTVQVGIT
                     FTSIACPCTDVLREDVEARLRRLDGVAHVEVEEVFEAWSREDMTEDGRLALLALGVT"
     gene            complement(7092..8024)
                     /locus_tag="VFO47_09140"
     CDS             complement(7092..8024)
                     /locus_tag="VFO47_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET8788900.1"
                     /translation="MLKTEAELLERLRTGRLIEGLDTATEAYVEGLKRTLIVSADTEL
                     ISAPAYLRAARDAPSINSYMSAVSIIQDELGHAHIAYRMLRDLGVDTDQLVYEREPKA
                     FKYPYAFDVPLDRWEELVVANAFYDRAGFVLLSDIYQNTSYGPWKRALVKVDREETFH
                     LRHGERWMKILADDPDRKAAVQRAVDWMFVLTLEWFGLPDDLKQHKEQVGYQLKGQSN
                     DQLRQTWMAATVPLCERLGFSVPAHLDQASGQYVIDCPFPMRFDEDAKRWDLGRGAIS
                     WDDVLVRWRRRGPCNEEFVGMLQRGHRQLRRGPA"
     gene            complement(8080..>8451)
                     /locus_tag="VFO47_09145"
     CDS             complement(8080..>8451)
                     /locus_tag="VFO47_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8788901.1"
                     /translation="NRYGDPTPIDEVLQHVPDLKVVLAHGGRGWWYEAAAFLALAREN
                     VWIELSGLPPQRLPDYYARPGLRRLARKFIFGSDWPGVPGIAANARAVAKLDIDEQDL
                     PGVLAGNARMVYRGLDPMEVD"
BASE COUNT         1240 a         3174 c         2958 g         1079 t
ORIGIN      
        1 cgaggccggg ccggcgtcga ggacctcgac gcggtcacca cccacaaccc gttcgcccgt
       61 caacgacctg tggcccgacc cgaccgagcc ggccggatga ccctggcctc ggccaggtat
      121 cgcgggacca gccgctggta ctcgccggcg gcggtgcggc cccactggct cgcccggccg
      181 gacagctcgc gcttgacctg ggcgggaacg ccggcggcga gcacgccggg cgggatctgc
      241 gcgtcctcgg cgaccacgct gccggcggcg atcaccgccc ccctgccgac acgggcccgc
      301 tggaggacca ccgcgcccat cccgatcagg gcgccgcgct cgatcacgca gccctccatg
      361 gtggccaggt gaccgacggt cacctcatcc tccaggacgg tcggcaggcc ctcggcggcg
      421 tgcaggacgc agttgtcctg cacgttgctg ccggcgccga ccacgatcgg accgaaatcg
      481 ccgcgcagca ccgccccgta ccagacgctc gcgccggcgg ccacggtcac gtcgccgacc
      541 agcaccgcgg tgtgggcgac gaaggcggcc gcgtccacca ccgggcggcg tcccgcgaac
      601 tcgatctcca gcgccatctg gtctcagcct tcctcgtcgg ggtggtcggg ccggcggcgg
      661 gcaggcgccc ggacccgccg gccgccggcg ctggagcggg cctgccggac gagcacgcgg
      721 aggaagtccg ccacggccgg gaccgcgccc cgcttgaagc ccaggaagag cccgatgatc
      781 agcaggccgt agagcacggt tcgcagctgc ccggcgtcga ccaggagctc gtccaccacc
      841 gcgaacacga ccgtccccag cacgggtccg aggagggtcc ccaggccgcc gaaggcgagc
      901 atcaccagga cgcggacgtc gacgtgtccg aactcgaagg tcgaggggct gatgaacccg
      961 tcgctgtagg tgaagacggc tcccgtgatc cccagcatgg cggagccgat caggccggcg
     1021 tagacgcggt atcgggccac gtcgaccccg gtcagctcgg cggcgagctc gttgtcgcgc
     1081 agcgcgcgga aggcccagcc gacgtgggac cgctcgagcg cgcggaacac caccaggaag
     1141 cccaccagca tgcccaggaa caggtagtag gcggccactc gctcctgcag gaaggtgccc
     1201 tcgccggcgg cgacgaccag gccggtctcg ccgcccgtca gctcccgctg gcccagcacg
     1261 aggttggcga aggaaaggga gaaggcaagc gtcacgatcc cggtgaagat cacgcccagc
     1321 gagcgccgca ccgccaccca gctgaggagc ccgccaacgg ccgttgccag caccgtggcc
     1381 gccaccagcc caaaggccag ggggagcccg gcctcctcgg agaggatggc ggcggtgtag
     1441 ccgcccaccc cggccagcgc gccgacgcac aggaagagct ggttggtgct gccgaagatc
     1501 acgttgaagg cgacggcgta ggccccgaag acgagagcgt ccacggccag ccccatcatc
     1561 agccgcgagt cgcccagcca cagggggacc agggcgagga gggcgccggt gacggcgatc
     1621 ggcaggaacg cccggggcag cgagcgcggc ggcggggcgc tcacccccag cggcgcgccg
     1681 tcggacgact cgaccgtcgc gtcgggctcg gccgtcacgg ctacctcccc agcaggccgc
     1741 gtggccgcac gacgatggtg agcgccgccg ccgccaacag cacgatggtg gtgacgtact
     1801 ggccgatgta gaaggcgctc accgtgctga cgataccgag gagcaggccg gccaggaagg
     1861 ctccggcgac gctgcccagg ccgccgacga cggcgacgat cagggccagg atggtgaagt
     1921 cgaacccggc cgtgacggcg accggcgagg tcatcgaccg ggtctcggca accagtcccg
     1981 ccagggcgcc gctgagcgcg aagatgaggg tgcggaccgc cgccgggttg atcccgacca
     2041 gccgcgcgcc cacctcgtcc tggatcaccg agcgggtggc ccgccccaag gtggtgcggc
     2101 ggaagaacag cagcagggcg gcgcccaccg ccagcgagat gcccgaggcc cacaggcttc
     2161 ccacccgcat gcggacgccg aggatcggca cggcctcgcc gcccacctcc aggcgcaggg
     2221 ccccgctcgg atagcgccag gtcagcagcc cgtcgatgac gaaggccaca cccagggtga
     2281 ggagcaggcc caggagcacc cggtgctcgc ctcgctgccg gtacaccggc tgcatcaggg
     2341 tcctgtccag gacgacgccg ccggccgcgg ccaccagcag cccggccccg gccgccacca
     2401 gggccggcat gccggcggcg aggctgagcg ccaccaccat cgccgccagc acggccgcct
     2461 ggccgtacga caggttgagc acacccccca ggccgtagac cagggtcatg cccacgccca
     2521 gcagcccgaa ctggaggccg aggacgatcc cgtcgaggac gacggccctc atgccacccc
     2581 caggtaggcc cgccggacct cggggttgcg ctccaggtcc tcggaggcgc cctcgaggcg
     2641 gacgcggccg ttctccagca cgtagccccg gtcggccagg gacagcgcca gcggcacctg
     2701 ctgctcggcg accagcagcg tcacgcccgt ctccttcagc gcctgcagcg cctggtacgc
     2761 gctccgggcc agcttggggg ccaggcccgt ggtgggctcg tcgagcatca gcaggcgggg
     2821 gcgggacatg agcgcccggc ccaccgccag catctgctgc tcgccgccgc tgaggctgcc
     2881 cgccggctgc ctggggcgct ccttgaggcg ggggaacagg tcgaagacga gctcgaagac
     2941 ggacgggtcg ggccggcccg gataggcgcc gagcaacagg ttctcccgca cgctcatgcc
     3001 ggggaagagg tgccgctcgg ccggcacgta ggccaggccg gcccgcgcga cgcggtgggc
     3061 cggccagccg gtgatgtccc tgccgtcgaa gcgcacccgg cccgcggaga ccggcagcat
     3121 cccgcagatg gcccggaaca gcgtcgtctt cccggcgccg ttggagccga tgacggcgac
     3181 ggcctcgccc gcggcgaccc gcaggtcgac gccttccacc acgttctccc ccaggtagcc
     3241 ggcctccagg ccggacgcct caagcatcgt ggccctcgcc gaggtaggcg tcgatcacct
     3301 cggggtggcg catcacggcc tccggctcgc cctcggccag catcctgccg aagttgagga
     3361 cgatcacccg ctcggccagg cgcatcaccg cctgcatcac gtgctccacc cagatgatgg
     3421 tgatcccgag ggtgtcgcgg gcggtgcgca cgatctgcac ggaggcggcc agctccgcgt
     3481 cgttgagccc cgccatcacc tcgtcgacca gcagcagccg cggccggccg gccagcgcct
     3541 tggccatctc caggaaccgc tgctggtgga gattgaggct ggcgacgggc tggtcggcgc
     3601 ggcccgcgag gccgacgaag gccagcgcct cctgcgcgcg ccggatcgcc tccggctccg
     3661 ccacgacgcc gtcgccgccg ccgaacatgg cgcccagcgc gacgttctcc aggactgtca
     3721 tggacccaaa ggtcagcggg gtctgcatca ccatcgcgat cccccgctgc cggacccggt
     3781 gaggggccag cccgtccacc cgctcgccgg ccagcgtcac cgtcccggcg gaggggcgct
     3841 cgaggccgcc gatggtcttc agcagggtgc tcttgccggc gccgttgggg ccgacgacgg
     3901 cgaggatctc gccctcgtcg accccgaacg tgacgccgtc gaccgccggg agaccgccga
     3961 agcgcttctc caccgcgtcg accgcgagca gcgccatgtc cccgctacga cggcggccgg
     4021 aacggctcga gcggctcgga gaccagcagc cgctccgggt accaggcgcc tgcctcgttc
     4081 accccctccg gggccgggcc gcggccgatc aggtcgaacg cgatccgggc gtcggccagc
     4141 tcgccccact ccgtccagcg cagctcgaag gagtagccgg ggatgtcgaa ggtgttctcg
     4201 tgcaggtggc tggcgatggc ggcgggatcg tcgcccgccg cgccgaccgc ctgggccact
     4261 gtcgtgacga tcccgtagcc ggccagggcg tcgtcctcga agaacttgtt gttctgggag
     4321 aacgccagga agcgtcgggc caggtcctgg tactcgttgc tctggacgtc ggcgcaaccg
     4381 aagtcggcgt agcggtcgag ggccgcgccc ttggccccgc tcatcaccag cgagcgcggg
     4441 gtgtaggcgc cggtgaccgg cacgtcgaag ccgaggtcgg ccgactggac ggtgatcgcc
     4501 ccggcgccgg gcggatgccc ggtggccacg atcatctgcg ggtcgaggcg ctccagcgaa
     4561 cggaggtagg tggtgaagtc ctgctcgggc accggggcaa cctcgatctg gagcttgatg
     4621 tcgggggccg ccgcgaacgc gtcctccagc gcccccttga tcgcctgtcc ccaggcgtag
     4681 tcggcgacga tcgcacccac cctggtcagg ttcttgctct gcacgtactc gaggatgggg
     4741 ccggcgacca tgggcgcggc gggcaggcag gtgcggaagg tgtgccggct gttggcgttc
     4801 aggatgccgc ccgctccggc cttcaccagg aacagcggga ccttgctctg ctccgcgatc
     4861 cgagccgtgg ccagcccgac gtcgctcgag atgatcccgc cgaccgccac cacgccgtcc
     4921 cgctcgatca gccgctcgag cgcgcgggtc ccttcctcgg ccttggactg gtcgtcggcc
     4981 accaccagct ccagctggcg gccgtcgacg ccgccgcgct ggttgatctc gttcaccgcg
     5041 agttgcatcc cgtcgcgcac ctgcacgccc catggcgcga agttgcccgt cagcgatgtg
     5101 agcgcgccga tgcggatcgg ctcgccgccg ccctcctggc ctcctccctc gtcgccgtca
     5161 ccgcacgccg ccagcagcag ggccgccacc agccagagcg agaacagccc gaccctggaa
     5221 cgatgcctca tttcctcctc ctgtctccgg gcttccgctg cgacgggtca gaacctccga
     5281 ctgactggtc agacttgccg gaccatatca gcagcctgac cagccagtcg aagagtctgt
     5341 gctcctgccg ccggcatggc cccgggcggc aggcgcgcga catcttcgcc gaggtccaca
     5401 acgggctggg ctcgctcgcc gagatgcgcc gtggcggacg agcggcgcct cggtcacgac
     5461 cggccgcgcc tgcggatccg ctcgaagggg ctcttgcagg cggtgcagat ccattgggag
     5521 gtcatcaccg tcggcccgac ctcgccgacc cgctcgaccg cctcggagcc gcaccacggg
     5581 cagctcacgc ggtcgccggc tccgggtcca gccggcgttg caggcggttc catcgctccc
     5641 atggcagccg agaatcctcc gatcgttcgg gacgcagccg gacgccgacc tcctcgagca
     5701 gggggccgac ccggtcgagc caggcctggc gcagctcggt gttgccccga ccgagcagcc
     5761 cgtggccgcg cagcctctcc actccctgct cgccgggggg accgaaccag cacagcagct
     5821 cctccaggga ctcgtccacg cgcgcctgga gctgcgggag gccgccgggc agccgggcca
     5881 gctcgcggac gcggccttcg gcgaagtccc agtggaaggc ctcctcctcc agcatccggg
     5941 gcacgcgtct ggccagcgcc tcgtagggcc cgccgatcat cgcctccagc atggtggtga
     6001 cggcgctgtc gaccagcagc agcgccgaca ccgcatgcgg ccaggtcggg aacggcctgt
     6061 cgagcagcga gacctggtag gcgcgctcgc gttcggtcgg gtcgaccggg ccgggaaacg
     6121 gcagctcgtc cagcagcggg tacagcaccc gggcctggcc gagctcgccc tgggcgatgg
     6181 cggcgctagc caccgccacc tccaggctcg gcgcccccac cgcccactcc gacaggtgcc
     6241 ggccgagcca gtacttgttg tcggccagga cgaccagcag gttcaccagg gcggccaggg
     6301 cctcgtcgtc gtcggtcacc caggcggtcg cgttgcccgc ccccagctca cccatcggtg
     6361 gacccctccg gcgacggcac cggggcctgc gggccgagga tccagtggat cgcccgctcg
     6421 gggatcagca ccagctccac ccaccggggg ccgtggcgct ccagcgccgc cctggctgcg
     6481 gcgtcgtcgt cggccgcggt cagcgtctcc tggaactcca gcggctccgg gtagctggtg
     6541 cggccgaaca ggcgataccg ctccaccggc cgcacctact cgggggcgac gacggggacg
     6601 acggccgcgc gcggcaccac gaacatctcc tgccagcggc gctcgtcgta catcatgaag
     6661 gcgaagacgc gggcgtcctt gggcccgacc gcccggaccg tgcccacgtg gtgggcccgc
     6721 tcctcacggt cgtggcgcgc gtagacctcc cagacctgtc gcctcgggtc gtcccccatc
     6781 cgcctacgtc acccccaggg cgagcagggc caggcggccg tcctcggtca tgtcctcacg
     6841 gctccaggcc tcgaagacct cctccacctc gacatgggcg acgccgtcca gccggcgcag
     6901 gcgcgcctcg acgtcctcgc ggagcacgtc ggtgcagggg caggcgatcg aggtgaacgt
     6961 gatcccgacc tgcacggtgg tgccgtcgac gaccaccgac cggaccagcc ccaggtcgac
     7021 gatgccgacc ggcagctcgg ggtcctcaac ctcggcgagg gcggcgcgga cgtcgtcgac
     7081 cgagaccatg gtcacgcggg gccccgccgc agctggcgat gcccacgctg gagcatgccg
     7141 acgaactcct cgttgcacgg cccgcgccgc cgccagcgca ccagcacgtc gtcccaggag
     7201 atggcgcccc gcccgaggtc ccaacgcttg gcgtcctcgt cgaagcgcat cgggaaggga
     7261 cagtcgatca cgtactggcc gcttgcctgg tccaggtggg cggggaccga gaagcccagc
     7321 cgctcgcaca gcggcaccgt ggccgccatc cacgtctgcc gcagctggtc gttgctctgg
     7381 cccttgagct ggtagccgac ctgctccttg tgctgcttga ggtcgtcggg gagcccgaac
     7441 cactccaggg tgagcacgaa catccagtcg accgcccgct gcaccgccgc cttgcggtcc
     7501 gggtcatcgg cgaggatctt catccagcgc tcgccgtggc gcaggtggaa ggtctcctcc
     7561 cggtcgacct tgaccagggc ccgcttccac ggcccgtagc tggtgttctg gtagatgtcg
     7621 ctgagcagca cgaagccggc gcggtcgtag aaggcgttgg cgaccaccag ctcctcccag
     7681 cggtccagcg gcacgtcgaa ggcgtatggg tacttgaacg ccttgggctc gcgctcgtag
     7741 accagctggt cggtgtcgac gcccaggtcg cgcagcatcc ggtaggcgat gtgggcgtgg
     7801 ccgagctcgt cctggatgat gctcacggcc gacatgtagg agttgatcga gggggcgtcc
     7861 ctggccgccc gcaggtaggc gggggcgctg atcagctcgg tgtcggccga cacgatcagg
     7921 gtgcgcttga ggccctccac gtaggcctcg gtggcggtgt ccaggccttc gatcagcctg
     7981 cccgtcctca ggcgctcgag cagctcagcc tcggtcttca gcatctcgct cctccggccg
     8041 gccctgcgac gcccctcatc atggcctcca tctgaccggt cagtcaacct ccatggggtc
     8101 gaggccgcgg tagaccatgc gggcgttgcc ggccagcacg ccgggcaggt cctgctcgtc
     8161 gatgtcgagc ttggcgacgg cccgggcgtt ggcggcgata ccgggcacgc cgggccagtc
     8221 gctgccgaag atgaacttgc gggccaggcg gcgcagaccg ggacgggcgt agtagtcggg
     8281 cagccgttgc ggcggcaggc ccgacagctc gatccagacg ttctcgcggg ccagggccag
     8341 aaacgccgcc gcctcgtacc accagccccg gccaccgtgg gccagcacca ccttgaggtc
     8401 ggggacgtgc tgcagcacct cgtcgatcgg ggtcgggtcg ccgtagcggt t
//
LOCUS       DASSWR010000544         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_923904,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000544 DASSWR010000000
VERSION     DASSWR010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923904"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..>2013)
                     /gene="valS"
                     /locus_tag="VFO47_09150"
     CDS             complement(29..>2013)
                     /gene="valS"
                     /locus_tag="VFO47_09150"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET8788902.1"
                     /translation="TAVAQAEVEDREVPGVAVQVRFRRDGAGPEGSGEPRGGAPVDRD
                     LVVETTRPELLAACVALVAHPSDDRYRDLVGTTATSPLFGVPVPVVAHRLADPAKGTG
                     LAMICTFGDLTDVLWWRELRLPVRSVVTRDGRLRETPPEGVPAGPAWAELAGRTTTQA
                     RRRVVELLEDAGDLVAGPRPVTHPVKFYENGDRPLEIVTSRQWFIRLLDHRQRLLERG
                     RELDWQPPFMRARYEHWVTGLHSDWLVSRQRFFGVPIPLWYPLTEAGEVDHDRPLVPG
                     EARLPVDPSTDVPDGYREADRGRPGGFVADPDVFDTWATSSLTPQIAGGWEEDPERFA
                     RVFPMDLRPQSHEIIRTWLFYTVTRSELEHGVLPWSRAAISGWVVDPDRRKMSKSKGN
                     AVAPTAVLERYGADALRYWAAKVRPGTDTTADEGQMRVGRRLAIKLLNSARFILGLPG
                     DGPDTGDPAEALDLAMLAGLDATVAEATAALEGADWTAALEATERCFWSFCDDYLELV
                     KDRAYRPVGEPGGASARAALRAALDAVARLLAPYLPYACEEVWSWWHDGSVHLASWPA
                     PELAPAGADPTPLAAASLVIAAVRRAKSQAKLPLRAPVALAVVEGPPGLLAAARAAEA
                     DLAAAGRVAAFEYRERPQAATLEVRVELAASEEATT"
BASE COUNT          247 a          773 c          766 g          227 t
ORIGIN      
        1 accccttcga cctcccgcaa tccctggctc atgtggtcgc ctcctcggac gcggccagct
       61 ccacccgcac ctccagcgtc gccgcctggg gacgctcacg gtactcgaac gccgccaccc
      121 gcccggccgc ggccaggtcg gcctcggccg cccgggcggc cgccagcagc cccggcgggc
      181 cctcgaccac ggccagggcg accggggccc gcaggggcag cttggcctgg ctcttagccc
      241 gccggacggc cgcgatgacc aggctggccg cggccagcgg ggtcgggtcg gcgccggccg
      301 gggccagctc cggcgccggc caggacgcca ggtggacgga gccgtcatgc caccacgacc
      361 agacctcctc gcaggcgtag gggaggtagg gggcgagcag gcgcgccacg gcatcaaggg
      421 cggcgcgcag ggccgcccgg gccgaggcgc cgccgggctc ccccacgggc cggtaggcgc
      481 ggtccttgac cagctccagg tagtcgtcgc agaaggacca gaagcagcgc tcggtcgcct
      541 ccagggcggc cgtccagtcg gccccctcca gcgcggcggt ggcctcggcc acggtggcgt
      601 ccagcccggc cagcatggcc aggtcgagcg cctcggccgg gtccccggtg tccggcccgt
      661 cgccggggag gccgaggatg aaccgggccg agttgagcag cttgatggcc aggcggcggc
      721 cgacccgcat ctggccctcg tcggcggtgg tgtcggtgcc cggccggacc ttggccgccc
      781 agtagcgcag ggcgtcggcc ccgtagcgct ccagcacggc cgtgggggcg acggcgttcc
      841 ccttggactt ggacatcttg cgccggtcgg ggtcgaccac ccagccggag atggccgccc
      901 gcgaccaggg cagcacgccg tgctcgagct cggaccgcgt caccgtgtag aacagccagg
      961 tccggatgat ctcgtgggac tggggccgca ggtccatggg gaacacccgg gcgaaccgct
     1021 ccgggtcctc ctcccagccg ccggcgatct gcggggtgag cgaggaggtg gcccaggtgt
     1081 cgaacacgtc ggggtcggcc acgaacccgc ccggccggcc ccggtcggcc tcccggtagc
     1141 cgtcgggcac gtcggtggac gggtccaccg gcagccgggc ctcgccgggg accagcggcc
     1201 ggtcgtggtc gacctcgccg gcctcggtca gcgggtacca cagcgggatg ggcacgccga
     1261 agaaccgctg gcggctgacc agccagtcgc tgtgcaggcc ggtcacccag tgctcgtagc
     1321 gggcgcgcat gaacggcggc tgccagtcca gctcgcggcc ccgctccagc agccgctggc
     1381 ggtggtcgag caggcggatg aaccactggc ggctggtcac gatctccagg ggccggtcgc
     1441 cgttctcgta gaacttcacc gggtgggtga ccggccgggg gccggcgacc aggtcgccgg
     1501 cgtcctcgag cagctccacc acccgccgcc gggcctgggt ggtggtcctg ccggccagct
     1561 cggcccaggc cgggccggcg ggcacgccct cgggcggggt ctcccggagc cggccgtcgc
     1621 gggtgacgac cgagcgcacc ggcagccgca gctcccgcca ccagagcacg tcggtgaggt
     1681 cgccgaaggt gcagatcatg gccagcccgg tccccttggc ggggtcagcc agccggtggg
     1741 cgaccaccgg gaccggcacc ccgaacagcg gcgaggtggc ggtcgtgccg accaggtcgc
     1801 ggtagcggtc gtcgctgggg tgggcgacca gggccacgca ggccgccagc agctccggcc
     1861 gggtcgtctc caccaccagg tcccgatcca ccggggcacc ccctcggggt tccccggacc
     1921 cctccggccc ggccccgtcg cgccggaacc ggacctggac ggccacgccg gggacctccc
     1981 ggtcctcgac ctcggcctgg gccacggcgg tgc
//
LOCUS       DASSWR010000545         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_218115,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000545 DASSWR010000000
VERSION     DASSWR010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218115"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFO47_09155"
     CDS             complement(<1..861)
                     /locus_tag="VFO47_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8788903.1"
                     /translation="MLTAEQNAELTRVGPGTPMGELLRRYWYPVAFTRQLEEFPVKRV
                     RLLGEDWALYKTRDGSYGVVEEHCPHRRASLVYGVVETEGIRCGYHGWLFGRDGACLD
                     QPAELDKTSFKDRVRARAGRAQELGGMVWVYVGPEPAPLLPRYDVYVMDGVRDFGHAL
                     LPCNWLQIMENAVDPHHVEWLHGRYFEFLGNRMGFEAPKSFQRRHVKVAFDEFEHDII
                     KRRLLEGHSEDDDDWAIGHPMVFPYCMRVGGAGIEQMQIRVPVDDTTTWFVLYTVHAP
                     DGAPAPAQAAI"
     gene            complement(874..2691)
                     /locus_tag="VFO47_09160"
     CDS             complement(874..2691)
                     /locus_tag="VFO47_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET8788904.1"
                     /translation="MADRFPSPFEIETPAGAEGWQELYTYSLPFSTDRREYEDSMFWF
                     QDGVHWPEVMSPWDATFYEFALASLSQYNTRHYLIPPALGVDYRVLNGYAYLSPVGVA
                     DPAEIEARVPHFLERAGFYFQNWASLYEQWMGKIRGLIGEMEALSFEPLPDKEELAVV
                     TEGHGLGSGFELQRSYHRLVDLALQLWQYHFEFLNLGYAAYLDFFGFCKQAFPSIPDL
                     AIAKMVAGVEVDLFQPDEELKKLARLAVDSRVDDAFQRSSAAEVIEALRGSDAGQVWL
                     SQWEASAQPWFNFSSGSGFYHTDEVWLEHLDIPFGFVRDYTAKLREGVDIGRPIEAIR
                     AERDRIVDEYGELLATDEDRAAFEQKLGLARVVFPYVENHNFYVEHWSHSVLWRKMRQ
                     LGGVLAKEGFFGADDDIFLLKRDEVPEALWDYYSAWAVGTPPRGPAYWGRELERRRGI
                     LTALRSWSPPPALGRPPEVVTEPFTIMLWGITGESVKQWLGAGDGSGDGGLKGFAASP
                     GVAEGPARVIFSADQIGEVQAGEILVAPLTAPSWAPIFGKIGATVTDVGGMMAHAAIV
                     CREYGLPAVTGTAFGTKTIKTGQRIRVDGNQGTVTILEA"
BASE COUNT          412 a          971 c         1006 g          411 t
ORIGIN      
        1 gatggcggcc tgggccgggg ccggggcgcc gtcgggggcg tgcaccgtgt agagcacgaa
       61 ccaggtggtg gtgtcgtcga ccgggacccg gatctgcatc tgctcgatcc cggcgccccc
      121 gacccgcatg cagtagggga acaccatcgg gtggccgatg gcccagtcgt cgtcgtcctc
      181 gctgtggccc tccagcagcc ggcgcttgat gatgtcgtgc tcgaactcgt cgaaggcgac
      241 cttgacgtgg cggcgctgga acgacttggg ggcctcgaac cccatccggt tgccgaggaa
      301 ctcgaagtag cggccatgca gccactcgac atggtggggg tcgacggcgt tctccatgat
      361 ctggagccag ttgcagggca gcagggcgtg gccgaagtcg cgcaccccgt ccatcacgta
      421 cacgtcgtag cggggcagca gcggcgccgg ctccgggccc acgtacaccc agaccatgcc
      481 gcccagctcc tgggcccgcc cggcccgggc ccggacccgg tccttgaacg aggtcttgtc
      541 gagctcggcc ggctggtcca ggcaggcgcc gtcgcggccg aacagccagc cgtggtagcc
      601 gcagcggatg ccctcggtct cgaccacccc gtagacgagc gacgcccgcc ggtgggggca
      661 gtgctcctcg accaccccgt aggacccgtc ccgggtcttg tacagggccc agtcctcgcc
      721 gagcagccgc acccgcttca ccgggaactc ctccagctgg cgggtgaagg cgaccgggta
      781 ccagtagcgg cgcaggagct cgcccatggg cgtgcccggc ccgacccggg tcagctcggc
      841 gttctgctcg gcggtcagca tgcgacggcc tccttacgct tccaggatgg tgacggtgcc
      901 ctggttgccg tccacgcgga tgcgctggcc ggtcttgatg gtcttggtcc cgaaggcggt
      961 gccggtcacg gccggcaggc cgtactcgcg gcagacgatg gcggcgtggg ccatcatccc
     1021 gccgacgtcg gtgacggtgg ccccgatctt gccgaagatc ggcgcccagc tgggggcggt
     1081 caggggggcg accaggatct cgcccgcctg gacctcgccg atctggtcgg ccgagaagat
     1141 cacccgggcc gggccctcgg ccacccccgg ggaggcggcg aagcccttga ggccgccgtc
     1201 gccggacccg tcaccggcgc ccagccactg cttgacgctc tcgccggtga tgccccagag
     1261 catgatcgtg aacggctcgg tcacgacctc gggcgggcgg ccaagggcgg gcggcggcga
     1321 ccaggaccgc agcgcggtca ggatgccccg gcggcgctcc agctcccggc cccagtaggc
     1381 ggggccgcgg ggcggggtcc cgaccgccca ggcgctgtag tagtcccaca gggcctcggg
     1441 gacctcgtcc cgcttgagca ggaagatgtc gtcatcagcg ccgaagaacc cctccttggc
     1501 caggacgccg ccgagctggc gcatcttgcg ccacagcacc gagtggctcc agtgctcgac
     1561 atagaagttg tggttctcga cgtaggggaa cacgacccgg gccaggccga gcttctgctc
     1621 gaaggcggcc cggtcctcgt cggtggccag cagctcgccg tactcgtcga cgatccggtc
     1681 gcgctcggcc cggatggcct cgatgggccg gccgatgtcg accccctcgc gcagcttggc
     1741 ggtgtagtcg cgcacgaacc cgaacgggat gtccaggtgc tccagccaga cctcgtcggt
     1801 gtggtagaag ccgctgccgg acgagaagtt gaaccacggc tgggccgacg cctcccactg
     1861 cgacagccac acctggccgg cgtcgctgcc ccgcagggcc tcgatcacct cggccgccga
     1921 ggaccgctgg aaggcgtcgt cgaccctgga gtcgacggcc aggcgggcca gcttcttcag
     1981 ctcctcgtcg ggctggaaca ggtcgacctc gaccccggcg accatcttgg cgatggccag
     2041 gtcggggatg ctggggaagg cctgcttgca gaagccgaag aagtccaggt aggcggcgta
     2101 gccgaggttg aggaactcga agtggtactg ccacagctgc agggccaggt cgaccaggcg
     2161 gtggtagctg cgctgcagct cgaaccccga gcccaggccg tggccctcgg tgacgacggc
     2221 cagctcctcc ttgtcgggca gcggctcgaa gctgagcgcc tccatctcgc cgatgagccc
     2281 gcggatcttg cccatccact gctcgtacag ggaggcccag ttctggaagt agaagccggc
     2341 ccgctccagg aagtggggga cccgggcctc gatctcggcc gggtcggcga ccccgaccgg
     2401 ggacaggtag gcgtagccgt tcaggacccg gtagtcgacg ccgagggccg gcgggatcag
     2461 gtagtggcgg gtgttgtact gcgacagcga ggccagggcg aactcgtaga aggtggcgtc
     2521 ccaggggctc atgacctccg gccagtggac cccgtcctgg aaccagaaca tcgagtcctc
     2581 gtactcgcgc cggtcggtcg agaacggcag cgagtaggtg tacagctcct gccacccctc
     2641 ggcccccgcc ggggtctcga tctcgaaggg gctcgggaac cggtcggcca tctccttgtc
     2701 cttccgtcga ggcccgggtc acggcccggg cgcgtccgaa ccttcacgtg cgtggtcgcg
     2761 tgcgtgcagg ggcgacagca gggtgctgac gatgctggcc
//
LOCUS       DASSWR010000546         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_133837,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000546 DASSWR010000000
VERSION     DASSWR010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133837"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..174
                     /locus_tag="VFO47_09165"
     CDS             10..174
                     /locus_tag="VFO47_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HET8788905.1"
                     /translation="MRNQYGPDHGRTQVTVLSPRHAHLAVGCASTYSRLLLETLADPD
                     APWRRAAASP"
     gene            complement(716..1705)
                     /locus_tag="VFO47_09170"
     CDS             complement(716..1705)
                     /locus_tag="VFO47_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HET8788906.1"
                     /translation="MQDDLLYRFRLRTFALAGELGNVRAACRAMGIHPSTYYRWKRQL
                     DRYGPEILRPRERRVPRMANATSPLIEQRVIAFALGHPGFGPARIAAELARPKWGGIQ
                     LSTNGVWRVLRRHGLSTRAKRYGLVAGYAAPPAPERPQPAPERHLDVDHPGELVQLDC
                     FCIGRLSGTKGTVWQYTAIDVASAYTWATLQVTRRNPSATWTSQLARTVAADLAARGW
                     KLERVMSDNASEFRSATFQATIATLKARQTFIRAGRPQTNGCVERVQQTILDECWKPA
                     FARYLIPKQTGLRLDLERYLRYYNTERAHTGRWTRGRTPDQVLGKAKLWHKRR"
     gene            1825..2142
                     /locus_tag="VFO47_09175"
     CDS             1825..2142
                     /locus_tag="VFO47_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET8788907.1"
                     /translation="MIDELYAPELAGAARRWIMPFRASFPDVHMEIVELIAEGDKVVG
                     RFTCSATHLGEWLGHGPTGRRFERVNEVTILRLREGRIVHAWSLEDTLSRLRQLRLTS
                     QHG"
BASE COUNT          374 a          791 c          813 g          368 t
ORIGIN      
        1 gtcgccgagt tgcgcaacca gtacgggccc gaccacggcc gcacccaagt cacggtgctg
       61 tccccgcgtc acgcccacct ggcggtcggc tgcgccagca cctatagccg cctcctgctg
      121 gagaccctgg ctgaccctga cgccccatgg aggcgtgccg ccgcctcacc ctagcttcct
      181 gaccccacaa gagaaggctc gggcgattcc gcaggaacgt ccggactgac agactgccga
      241 aggtacgcag atgaatggtc gacgacggtg gatggctgcc atgatcacga cgagctgcac
      301 cactcacagg atcgacctgc gatgaccaac cgtgacctgg cgaacgcaac cgggctggcc
      361 gcatggatcg tcgccaacga ttaccgcccc catatgaccc gcaagatggt cctgcgactg
      421 gcgcagaccg acccgaaggc acccaagccg gtgccaagcg ccggctccga gaccgtctac
      481 tcgctgtccc agtggcactg ctacctcgac cccaccgccg atcccagcca ggcccaccgg
      541 tatctcggca ccgaagctca cccggacttg gtggacgcag acgagatggc gcgacgcacg
      601 gtcgccgacg ggtacgcgcc gaccatgagc cgacagcggg tgctgtggct ggccaggact
      661 gaccccgact tccccgacac cctaacctgt cctgtccccg agttgtggcg acgcatcagc
      721 gcctcttgtg ccacagcttg gccttcccaa ggacctggtc tggggtgcgg ccgcgggtcc
      781 agcggccggt gtgcgcccgc tcggtgttgt agtaccggag ataccgctcg aggtcgagcc
      841 tgaggccggt ctgcttggga atcagatagc gggcaaaggc gggcttccag cactcgtcca
      901 ggatggtctg ctggacgcgt tccacacacc cgttcgtctg cggccggccg gcccggatga
      961 aggtctgtcg ggccttgagg gtggcgatgg tggcctggaa ggtagccgag cggaactctg
     1021 aggcgttgtc gctcatgacc cgctccagtt tccagccgcg ggcggccagg tcagccgcga
     1081 ccgtgcgggc cagctggctg gtccaggtgg ctgaggggtt gcggcgagtc acctggaggg
     1141 tggcccaggt gtaggccgag gccacgtcga tggcggtgta ctgccacacg gtgcccttgg
     1201 tgccactgag gcggccgatg cagaagcagt cgagctggac cagttcaccg ggatggtcaa
     1261 cgtccaggtg ccgctcaggt gccggttgcg gccgttcagg agccggtggg gcggcatagc
     1321 cggcgaccag cccgtagcgc ttggcgcggg tgctcaggcc gtggcggcgc aggacccgcc
     1381 acaccccatt ggttgacagc tggatgccgc cccacttggg ccgggcgagc tcggcggcga
     1441 tccgggccgg gccaaagccg ggatggccga gggcgaaggc gatcactcgc tgctcgatga
     1501 gtgggctggt ggcattggcc atgcgcggca cccggcgctc acgagggcgc aggatctcgg
     1561 ggccatagcg gtccagctgc cgcttccacc ggtagtaggt ggagggatgg atgcccatgg
     1621 cccggcacgc cgcccgcacg ttccccagct cgccggcgag ggcgaaggtt cgtagacgaa
     1681 accggtacag caggtcatcc tgcatcacgg cgacgcctcc ttacggcttg acttggtcgt
     1741 cagcgcgaag gatgcgtcgc cgctcagcct ctgtggggga cctgccccgg cgtcgctaca
     1801 tctcggggac aggacaacct agaggtgatc gacgagctgt acgcgcccga gctcgccggg
     1861 gcggccaggc gctggatcat gccgttccgg gccagcttcc ccgacgtcca catggagatc
     1921 gtcgagctga tcgccgaagg cgacaaggtc gtcggccgct tcacctgctc ggccacccac
     1981 ctgggggaat ggcttgggca tgggccgact gggcgccgct tcgagcgggt caatgaggtc
     2041 accatcttgc ggctgcgcga gggcaggatc gtccatgcct ggagccttga ggacaccctg
     2101 tcgcggctgc ggcaactccg actcacctcc cagcacgggt agcacgccgg cgtcagtcct
     2161 accgagcgcg acctgctggg ccgttgccgt gccagtgcac gctccggctt gatcactccg
     2221 agctggtggg gtgtccgtca ggggccggcg tctctggcgc attgacgact ggccacgtcc
     2281 agccgggcca tactcgggga tcgatcccgc tgccgagggc cagcgatgtt ggaccggagc
     2341 gaggag
//
LOCUS       DASSWR010000547         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_471682,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000547 DASSWR010000000
VERSION     DASSWR010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471682"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFO47_09180"
     CDS             complement(<1..256)
                     /locus_tag="VFO47_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HET8788908.1"
                     /translation="MRRAQLIGIVLVAVLVLAGCGSLTPVTEEGVEIRDLYNLLFVAA
                     AVVFVLVEAAIVFAVLRYRRRGDMLPPQFHGNNLLEVVWTV"
     gene            complement(310..2088)
                     /locus_tag="VFO47_09185"
     CDS             complement(310..2088)
                     /locus_tag="VFO47_09185"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET8788909.1"
                     /translation="MSLFRRLAVVTIATTFVLIAVGGLVRATDSGLGCPDWPRCFGKL
                     VPPAELHAWIEHSHRLVASVVMVLVAWLVVAAWRTGQDRVVRQAAVAALALVLAQALI
                     GALVVWWKLRADSVTLHLATALALVALLIFIDFRARPGGGRRQGQDRRFVRLVAAGAG
                     LLYLQMLVGSTVTGHHAGLAYPLDVLWPDLGPSVARIQLAHRTLAVVVGTLVVAIWLV
                     ARRSQRDHPTVTRLAGYAAGLVAVQIGLGVANVANRLSAVTVVPHLAVGALLWGTLFA
                     LLLHADRFAGLPGRDPAEPEPAPARGARQSATAYFLLTKPRIIELLLVTTVPTMFIAA
                     RGVPSPWLMAATLFGGALSAASANVLNCYLDRDIDALMRRTARRPLPAHRVDPADALR
                     FGLVLGVAGFVWLWTFVNLLSAVLATSAILFYVFVYTIGLKRRSTQNIVIGGAAGAVP
                     VLVGWSAVTGRVDLPALVLFAIIFYWTPPHFWALSLRFKDDYAAAGVPMLPVVRGARE
                     TSTQILYYTVLLVAVTLLLYPAGRMGAVYLAAAVALGGAFIWRALELRRDLTGRRAIR
                     LFSFSNTYLALLFCAMAVDAVVRGGA"
     gene            2131..>2704
                     /locus_tag="VFO47_09190"
     CDS             2131..>2704
                     /locus_tag="VFO47_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788910.1"
                     /translation="METRTADGRLAAWIFDLPVVELRSGYRAAVYFDRSRRILAAEGD
                     DTRVTMQVFQRSDAMVACGIDEALAVLALGAGSFVDPDAADRAFGTYLTARDAARRAR
                     SRGGDAWVEGVRAQVEAEAAFERHWRRADPDDLAVSALRDGDRIDAWEPVIELTGPYR
                     LVAHLESVYLGVLARRTLVASNVRQVVDAAG"
BASE COUNT          431 a          955 c         1030 g          288 t
ORIGIN      
        1 ccaccgtcca gacgacctcg agcaggttgt tgccgtggaa ctggggcggg agcatgtcgc
       61 cgcggcggcg gtagcggagc acggcgaaca cgatcgccgc ctcgaccagc acgaagacga
      121 ccgcggcggc gacgaagagc aggttgtaga ggtcacggat ctcgaccccc tcctcggtga
      181 ccggggtgag cgagccgcat cccgccagca ccagcaccgc caccagcacg atgccgatca
      241 gttgcgcgcg tcgcatcgga gtccggtcgc ctcgcttcct gggatgttcg agtcgggaac
      301 gactgtacct catgcccctc cccgcaccac cgcatccacg gccatcgcac agaacagcag
      361 ggcaaggtag gtgttggaga agctgaacag ccggatggcc cgccggccgg tgaggtcgcg
      421 gcgcagctcg agggcccgcc agatgaacgc ccccccgagg gccaccgccg cggccaggta
      481 caccgccccc atgcgcccgg ccgggtacag cagcagggtc acggcgacca gcaggaccgt
      541 gtagtagagg atctgggtcg aggtctcgcg cgccccccgg accaccggca gcatcggcac
      601 cccggcggcc gcgtagtcgt ccttgaagcg cagcgacagc gcccagaagt gcggcggcgt
      661 ccagtagaag atgatcgcga acagcaccag cgccggcagg tcgacccggc cggtcacggc
      721 cgaccagccg accagcaccg gcacggcccc ggcggccccg ccgatgacga tgttctgggt
      781 cgagcggcgc ttgaggccga tggtgtagac gaacacgtag aacaggatgg ccgaggtggc
      841 cagcacggcc gagagcaggt tcacgaacgt ccacagccac acgaacccgg ccacccccag
      901 gaccagcccg aaccgcaggg cgtcggccgg gtcgacccgg tgggcgggca ggggccggcg
      961 ggcggtgcgc cgcatcaggg cgtcgatgtc ccggtccagg tagcagttga ggacgttggc
     1021 gctggccgcc gagagggcgc cgccgaacag ggtggccgcc atcagccagg gcgacggcac
     1081 cccgcgggcg gcgatgaaca tggtcgggac ggtggtgacc aacagcagct cgatgatgcg
     1141 gggcttggtc agcaggaagt aggcggtggc cgactggcgg gcgccgcggg ccggggccgg
     1201 ctcgggctcg gccgggtcgc ggccgggcag gcccgcgaac cggtcggcgt gcagcagcag
     1261 ggcgaacagg gtcccccaga gcagcgcccc aacggccagg tgggggacca ccgtcacggc
     1321 cgacaggcgg ttggccacgt tggccacgcc caggccgatc tggacggcca ccaggccggc
     1381 ggcgtagccg gccagccggg tcaccgtggg atggtcccgc tggctgcgcc gggccaccag
     1441 ccagatggcc acgaccaggg tcccgacgac cacggccagg gtccggtggg cgagctggat
     1501 gcgggccacg ctggggccga ggtcgggcca gagcacgtcc agggggtagg ccagcccggc
     1561 gtggtggccg gtgacggtcg agccgaccag catctggagg tagagcaggc cggcgccggc
     1621 cgcgactagg cgcacgaagc gccggtcctg gccctggcgg cggccgccgc cggggcgagc
     1681 ccggaagtcg atgaagatga gcagggcgac caaggccagg gcggtggcca ggtgcagggt
     1741 gacggagtcg gcccgcagct tccaccagac caccaacgcc ccgatcaggg cctgggccag
     1801 caccagcgcc agggcggcga ccgcggcctg gcggaccacc cggtcctggc cggtccgcca
     1861 ggccgccacc accagccagg cgaccagcac catcaccacc gaggcgacca gccggtggga
     1921 gtgctcgatc caggcgtgca gctcggccgg ggggacgagc ttgccgaagc agcgcggcca
     1981 gtcggggcag cccagcccgg agtcggtcgc ccgcaccagg cccccgacgg cgatcagcac
     2041 gaaggtcgtc gcgatcgtga cgaccgccag gcggcggaac aagctcaacc actcctccgt
     2101 gtagccatgg acggagcata gtgttgcacc atggagacac gcacggccga cggccgactc
     2161 gccgcctgga tcttcgacct gccggtggtc gagctgcgtt ccgggtaccg ggccgcggtc
     2221 tacttcgacc ggtcccggag gatcctggcc gccgagggcg acgacacccg ggtgaccatg
     2281 caggtgttcc agcgctcgga cgccatggtg gcctgcggca tcgacgaggc cctggccgtg
     2341 ctcgccctgg gggccggctc gttcgtcgac cccgacgccg ccgaccgggc gttcggcacc
     2401 tacctgaccg cccgggacgc cgcccgccgg gcccgctcgc ggggcgggga cgcctgggtc
     2461 gagggggtgc gggcccaggt ggaggcggag gcggccttcg agcggcactg gcgccgggcc
     2521 gacccggacg acctggcggt gtcggcgctg cgcgacggcg accggatcga cgcctgggag
     2581 ccggtgatcg agctcaccgg cccctaccgg ctggtcgccc acctggagag tgtctacctg
     2641 ggggtgctgg cccgccggac cctggtcgcc agcaacgtgc gccaggtggt ggacgcggcc
     2701 gggg
//
LOCUS       DASSWR010000548         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_924049,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000548 DASSWR010000000
VERSION     DASSWR010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924049"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFO47_09195"
     CDS             complement(<1..290)
                     /locus_tag="VFO47_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET8788911.1"
                     /translation="MAEAGFERTELPGGLRVLTERMPAVRSVTLGIWAGVGSRDETPR
                     LAGASHYLEHLLFKGTRRRSARDIAEAMDAVGGETNAFTSKEYTCFYARTLDR"
     gene            complement(293..2485)
                     /locus_tag="VFO47_09200"
     CDS             complement(293..2485)
                     /locus_tag="VFO47_09200"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017575059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET8788912.1"
                     /translation="MQGAVGDRDLYLETGKLAKQAGGAVLIGLGETQLLVTATAAKHP
                     KEQLDFMPLTVDVEERMYAAGKIPGGFFRREGRPSETAILTARLVDRPLRPSFPKGMR
                     NEIHVVATIMSTDMENLSDVLAINGASAAVQLAGIPFSGPVGAVRLGRKAGDWIVNPT
                     YSELEECTFDMVVAGRQNEAGGVDILMVEAEATEQATHLMEETGAPKPTEEALGLALE
                     LAKTHIATACQLQVELVRQAGAQPREYPLFAEYGEDVAGAVRAAVEAQVREAITIADK
                     AEREVRLDELKAAAAQAATEQLGEALAGREKEASAAFRSLTKELVRQRVVKDAKRIDG
                     RGPRDIRPLEAEVGILPRAHGSGLFQRGETQVLNITTLGMVRMEQIVDTLDPEDRKRY
                     MHHYNFPPFSTGEVGFMRGPKRREIGHGALAERALLPVIPDKEEFPYALRLVSEVLSS
                     NGSTSMASVCASTLSLMDAGVPIKAPVGGIAMGLIAEGGEYVTLTDILGAEDAFGDMD
                     FKVAGTREFVTALQLDTKLTGIPSEVLIGALTQARDARMVILDVIQAAIDRPREQLNR
                     LAPRVHVEQIPVAKIGELIGPKGKRINEIQSTSGAEISIEDDGRVYIGATDGDQLDIA
                     LKMIREQMNPVMPQVGERYLGTVVKTTTFGAFISLTPGKDGLLHISRLGKKGQRVRAV
                     EDVVNVGDKLLVEVMEVDRQNRISLKLVDEDEAQSTSAPAADGGGEEG"
BASE COUNT          333 a          876 c          959 g          415 t
ORIGIN      
        1 cggtcgaggg tcctggcgta gaagcaggtg tactccttgg aggtgaaggc gttggtctcg
       61 ccgccgaccg cgtccatcgc ctcggcgatg tcgcgggccg agcgccgccg ggtccccttg
      121 aacagcaggt gctccaggta gtgggaggcg ccggccaggc gtggggtctc gtcacgggac
      181 cccacgccgg cccagatgcc gagggtgacc gagcgcacgg ccggcatgcg ctcggtcagg
      241 acccgcaggc cccccggcag ctcggtgcgc tcgaacccgg cctcggccac ggtcagcctt
      301 cctcgccgcc gccgtcggcg gccggggcgg aggtggactg cgcctcgtcc tcgtcgacca
      361 gcttcaggga gatgcggttc tgacggtcga cctccatcac ctcgacgagc agcttgtcgc
      421 cgacgttgac gacgtcctcg accgcccgca cccgctgccc cttcttgccg aggcgcgaga
      481 tgtgcagcag gccgtccttg cccggggtga gcgagatgaa ggccccgaag gtggtggtct
      541 tgaccaccgt gcccaggtag cgctcgccga cctggggcat gaccgggttc atctgctcgc
      601 ggatcatctt gagggcgatg tcgagctggt cgccgtcggt ggccccgatg tagacccggc
      661 cgtcgtcctc gatcgagatc tcggcgcccg aggtggactg gatctcgttg atccgcttgc
      721 ccttgggacc gatcagctcg ccgatcttgg ccaccgggat ctgctcgaca tggacccgag
      781 gggcgagccg gttgagctgc tcgcgcggac ggtcgatggc cgcctggatg acgtcgagga
      841 tgaccatgcg ggcgtcgcgg gcctgggtca gggcgccgat caggacctcg ctggggatgc
      901 cggtgagctt ggtgtcgagc tgcagggcgg tgacgaactc gcgcgtgccc gcgaccttga
      961 agtccatgtc gccgaaggcg tcctcggcgc cgaggatgtc ggtcagggtg acgtattcgc
     1021 cgccctcggc gatcaggccc atggcgatgc cgccgaccgg ggccttgatg ggcacgccgg
     1081 cgtccatcag cgacagggtg gaggcgcaca ccgaggccat cgaggtcgag ccgttggagc
     1141 tcaggacctc cgagaccagc cgcagcgcgt aggggaactc ctccttgtcc gggatcaccg
     1201 gcagcagggc ccgctcggcc agcgcgccgt ggccgatctc gcggcgcttg ggcccgcgca
     1261 tgaagccgac ctcaccggtg gagaagggcg ggaagttgta gtggtgcatg tagcgcttgc
     1321 ggtcctcggg gtcgagggtg tcgacgatct gctccatgcg gaccatgccg agggtggtga
     1381 tgttcagcac ctgggtctcg ccgcgctgga acagcccgga gccgtgggcc cggggcagga
     1441 tgccgacctc ggcctcgagc ggccggatgt cgcgcggccc gcggccgtcg atccgcttgg
     1501 cgtccttgac gacccgctgg cggaccagct ccttggtcag gctgcggaag gcggccgagg
     1561 cctccttctc gcggccggcc agggcctcgc cgagctgctc ggtcgcggcc tgggcggcgg
     1621 cggccttgag ctcgtcgagg cggacctccc gctcagcctt gtcggcgatg gtgatggcct
     1681 cgcgcacctg ggcctcgacc gcggcccgga cggccccggc cacgtcctcg ccgtactcgg
     1741 cgaacagcgg gtactcgcgg ggctgggcgc cggcctggcg gaccagctcg acctggagct
     1801 ggcaggcggt ggcgatgtgg gtcttggcca gctcgagggc gagcccgagg gcctcctcgg
     1861 tcggcttggg ggcgccggtc tcctccatca ggtgcgtggc ctgctcggtc gcctcggcct
     1921 cgaccatgag gatgtcgacc ccgccggcct cgttctggcg gccggccacg accatgtcga
     1981 aggtgcactc ctcgagctcg gagtaggtcg ggttgacgat ccagtcgccg gccttgcggc
     2041 cgagccggac ggccccgacc gggcccgaga acgggatccc ggccagctgc acggccgccg
     2101 aggcgccgtt gatggcgagc acgtccgaga ggttctccat gtcggtcgac atgatggtgg
     2161 cgaccacgtg gatctcgttg cgcatcccct tggggaacga ggggcgcagc ggccggtcga
     2221 ccaggcgggc ggtgaggatg gcggtctcgc tcgggcgccc ctcacgacgg aagaagccgc
     2281 cggggatctt gccggcggcg tacatgcgct cctccacgtc cacggtcagg ggcatgaagt
     2341 cgagctgctc tttgggatgc ttggccgcgg tggctgtgac caggagctgg gtctcgccca
     2401 gcccgatgag aacggcgccg cccgcctgct tggccagctt gccggtctcc agataaaggt
     2461 cacggtcgcc gacggcgcct tgcacacgat gaactgccat agatgtggat ctctccctct
     2521 tcctctgccg ccccgcgcct ggcggggcga acccatgccc ggccgcggtc gcggccggag
     2581 cgg
//
LOCUS       DASSWR010000549         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1150281, whole genome shotgun sequence.
ACCESSION   DASSWR010000549 DASSWR010000000
VERSION     DASSWR010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150281"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFO47_09205"
     CDS             complement(<1..456)
                     /locus_tag="VFO47_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788913.1"
                     /translation="MAPERKRSARDYEQALRRQELAAGRALVQAVGDQASPGARELAA
                     ARRVLTQALFDLRRLARDLDQEIEGLRAKGPRAARAAQGPGPGELDRLEALRGTVEEV
                     AERWEGRRDQLSAELDRRQPRQRQGQGRPADQRQPRGARDRGEVRNRRGA"
     gene            612..>2771
                     /locus_tag="VFO47_09210"
     CDS             612..>2771
                     /locus_tag="VFO47_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8788914.1"
                     /translation="MATCPNCETDLVEGARFCPGCGRPVGRLSGQEFRIVTVVFCDVV
                     KSTELERELDPQPMQRLLARYGEAVRRALGGRGASVGKRHGDGFMAAFGVPELHEDDA
                     LRAVRAAAELRAVLDELAKEVRQQRGLEFHVRLGINTGNVLVVRDADTLEEELTGTAV
                     NLAKRFEEAAAAGEILLGEETYRLVADAVKAEPAGPLTVKGADEPQEVWRLLEVLPDR
                     LGRARRAMAPMVGRGREQELLLRLFQRAAAEGTCHLVTVLGSAGVGKSRLVDEFVGGL
                     GDQAAVLLGHCPAFGDSVTMWPMVEVVRQAAGIASDESPEDARAKLAELVGAEERATL
                     VTERIAQLLGFGRQDAGLPEGTFWALQRLLETLARRRPLVLVVDDLQWADPILLDAVE
                     HIAEHADAPLVLLCMARPDELFTHRQHWPGGKANALSFLLSPLPEREGEQLVVHLLGG
                     RVDQAVQAHINEWAQGFPLLVEELVSNLRDEGWLRTADGRWTLQVESEEAADRRARSV
                     PTSIHTLLLARLDRLGPRGRMVIEPAAVVGQQFHFGDIKALHPDASAADLAAGLQELV
                     RLDLIRADHGPTSAPLPPNSGPGYRFRHATIKTVAYERLPDDRRAELHERYADWLERE
                     TEDRRSQFDEIVGHHFYEAYRYARRLDPGGEQMRELARRAGERYAVAGQRAAVRGDTR
                     LIQAWLGRAVRLLPAEHPLRLSALPPLAEAQQSSGKLT"
BASE COUNT          334 a         1073 c         1007 g          357 t
ORIGIN      
        1 agcgccccgt cggttcctga cctccccacg gtcccgggcc ccgcgcggct gccgctggtc
       61 ggcgggccga ccctggccct ggcgctggcg cggctggcgg cggtcgagct cggcgctcag
      121 ctggtcacgg cggccctccc agcgttcggc gacctcctcg acggtgccgc gcagcgcctc
      181 gagccggtcc agctcccccg gtcccgggcc ctgggcggcc cgggcggcgc gggggccctt
      241 ggcccggagg ccctcgatct cctggtcgag gtcgcgggcc agccgccgta ggtcgaacag
      301 ggcctgggtg agcacccgcc tggccgcggc cagctcgcgc gcccccggcg aggcctggtc
      361 gccgacggcc tggaccagcg cccgcccggc cgccagctcc tggcgccgca gcgcctgctc
      421 gtagtcccgc gcactccgct tgcgctccgg cgccaccgct ccactcctcg cttcctccga
      481 ccggctctcg ccagcgacag cgtagccagc cggggaccgt tcgagtaccc gaattcctga
      541 cgatcacgcc gctcgctgga actgtcagca acaggacggt tgccgtgccc gatcgtccat
      601 atactcctgc catggcgaca tgtcccaact gcgagaccga cctggtcgag ggggcccggt
      661 tctgtcccgg gtgcggtcgg ccggtcggcc ggctctccgg ccaggagttc cggatcgtca
      721 cggtcgtctt ctgcgacgtg gtcaagtcga ccgagctgga gcgtgagctc gacccgcagc
      781 ccatgcagcg gctcctggcc cgctacggcg aggctgtccg ccgggccctc ggcggccggg
      841 gggccagcgt cggcaagcgc cacggcgacg gcttcatggc cgccttcggc gtccccgagc
      901 tgcacgagga cgacgccctg cgggccgtgc gggcggccgc cgagctccgc gccgtcctcg
      961 acgagctggc caaggaggtg cgccagcagc gcggcctgga gttccacgtc cggctgggca
     1021 tcaacaccgg caacgtgctc gtcgtccgcg atgccgacac cctggaggag gagctcaccg
     1081 gcaccgccgt gaacctggcc aagcggttcg aggaggcggc cgcggccggg gagatcctgc
     1141 tcggcgagga gacctaccgg ctggtcgccg acgcggtcaa ggccgagccg gccgggcccc
     1201 tgacggtcaa gggcgccgac gagccgcagg aggtctggcg gctgctggag gtcctgcccg
     1261 accgcctcgg ccgggcccgg cgggcgatgg cacccatggt cggccgcggt cgcgagcagg
     1321 agctgctgct gcggctgttc cagcgggcgg ccgccgaggg cacctgccac ctggtcaccg
     1381 tcctcggctc ggccggggtc gggaagtcgc ggctggtcga cgagttcgtg ggcggcctgg
     1441 gcgaccaggc ggccgtgctg ctcggccact gtcccgcctt cggggacagc gtgaccatgt
     1501 ggccgatggt cgaggtggtc cgccaggcgg ccgggatcgc ctccgacgag tcgcccgagg
     1561 acgcccgggc caagctggcc gagctggtcg gcgccgagga gcgggccacg ctcgtcaccg
     1621 agcggatcgc ccagctgctc ggcttcggcc gccaggacgc cgggctgccc gagggcacct
     1681 tctgggcgct gcagcggctg ctggagaccc tggcccgccg ccggccgctg gtgctggtcg
     1741 tcgacgacct ccagtgggcc gacccgatcc tgctcgacgc cgtcgagcac atcgccgagc
     1801 acgccgacgc gccgttggtg ctgctctgca tggcccggcc ggacgagctg ttcacccacc
     1861 gccagcactg gccggggggc aaggcgaacg cgctgtcgtt cctgctctcc cccctgcccg
     1921 agcgggaggg cgagcagctg gtcgtgcacc tgctcggcgg ccgggtcgac caggccgtcc
     1981 aggcccacat caacgaatgg gcccagggct tccccctgct ggtcgaggag ctggtcagca
     2041 acctccggga cgaaggctgg ctgcggacgg ccgacggccg ctggaccctc caggtggagt
     2101 ccgaggaggc ggccgaccgg cgggccaggt cggtgccgac ctccatccac accctgctgc
     2161 tggcccggct cgaccggctc ggcccccgcg gccggatggt gatcgagccg gcggccgtgg
     2221 tcggccagca gttccacttc ggcgacatca aggccctcca ccccgacgcc tcggcggccg
     2281 acctcgccgc cgggctccag gagctggtcc gcctcgacct catccgagcc gaccacggcc
     2341 ccacctcggc gcccctgcct ccaaacagcg gtcccggcta ccggttccgg cacgccacca
     2401 tcaagaccgt tgcctacgag cggctgcccg acgaccggcg ggccgagctg cacgagcgct
     2461 acgccgactg gctggagcgc gagaccgagg accggcgtag ccagttcgac gagatcgtcg
     2521 gccaccactt ctacgaggcc taccgctacg cgagacggct cgacccgggc ggtgagcaga
     2581 tgcgggagct ggccaggcgg gccggggagc gctacgcggt cgccggccag cgggccgccg
     2641 tccgcggcga cacccggctc atccaggcct ggctgggccg ggcggtccgc ctgctcccgg
     2701 ccgagcaccc gctgcggctg tccgccctgc ccccgctggc cgaggcccag cagagcagcg
     2761 gcaagctcac c
//
LOCUS       DASSWR010000550         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_331844,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000550 DASSWR010000000
VERSION     DASSWR010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331844"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFO47_09215"
     CDS             complement(<1..796)
                     /locus_tag="VFO47_09215"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET8788915.1"
                     /translation="MEEIMQQHTRPDRPTPAESFPSDPSGLAEAGRPELLELAAGDTL
                     ELRVGPVAKRLGESRVRMLGYNGSVPGPTLKVAQGSEIVVHVINQGDLDTTVHWHGLR
                     LENRYDGVPHETQAPIPVGGEFSYRIQFPDAGLYWYHPHIREDYTQELGLYGNIVVVP
                     AEPGYWPPADRDFILTVDDLLVEEGRIAPFSPTETSYAAMGRFGNVFLIGGDPELRLS
                     AGVGEVVRLWLTNTANTRVFNLTLPGVRLKLVGGDSGRVEHEELVES"
     gene            complement(1118..>2935)
                     /locus_tag="VFO47_09220"
     CDS             complement(1118..>2935)
                     /locus_tag="VFO47_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET8788916.1"
                     /translation="LADRRVLFGLYHPTAARVFVAGSFNDWQHPGHQRPDPDAFVELE
                     LFRGWFGAANTWLAVADRAGAGDEYRFWVQGGTPPEDAAGGRLVVDPYARQLGPGDQR
                     SNAVVVDPTTFAWSDRGWRTPDPADLILYEVSVHGFTDGDPDIRPDQQGRFDGITERI
                     RTGYFDSLGVTALSLMPLAEVPSMQGPQSLGYDPSLYHAVERDFGGPDDLRRLVDAAH
                     GRGLALLVDMVFNHTSNDFNPLWDLILEQPDDAGGGLYFNGATPWGNRVATEKADVQN
                     LLIDSCRMLVGEYHVDGFRLDATHTDYMDHGFLHRLAAELKTGKPDVLLVAENLPNQG
                     DLNLEGWNGYGQWSELFHDKLKALLREGQFEGQSAGTDNLGDTFYFSRSRFAAHTNNV
                     VNYCESHDEQSVPYELQFTPWLDNPAAKERKGRLGMFATLVALGQPMIYMGQEFNLER
                     PRNVVAVDWPASLDQHGFYQWCRRLVRLRRRYPGLRLRGFDPAAAGQFEWILGPWLDE
                     SHGGGRRTVGWRAHPSDRPTDTLAVLLNFEGFDVQVDLELGRPGAWVKLADIDRVDDL
                     PPDGTNDPRDPTALHSTDGRFASFTLPGSSGFIYKWTTP"
BASE COUNT          444 a          982 c         1048 g          461 t
ORIGIN      
        1 ccgactcgac cagctcctca tgctcgaccc ggccgctgtc ccccccgacc agcttcagcc
       61 gcaccccggg cagggtcagg ttgaacaccc gagtgttggc ggtgttggtc agccacagtc
      121 gcaccacctc ccccaccccg gctgacagcc gcagctccgg atccccgccg atcaaaaaca
      181 cgttgccgaa gcggcccatg gccgcatagc tggtctcggt cggactgaac ggagcgatcc
      241 ggccctcctc gaccagcaga tcatccacgg tgaggatgaa gtcccggtcg gccggcggcc
      301 aatagccggg ctcggccggg acaaccacga tgttgccgta caggcccagc tcctgggtgt
      361 agtcctcccg gatgtggggg tggtaccagt acaacccggc atcggggaac tggatccgat
      421 agctgaactc cccccccacc gggatcgggg cctgggtctc atgggggacc ccgtcatagc
      481 ggttctccag ccgcaaccca tgccagtgca cggtcgtgtc cagatccccc tggttgatga
      541 catgaaccac aatctcgctg ccctgggcga ccttgagggt cggacccggg accgaaccgt
      601 tgtagcccaa catccgcacc ctggactcgc ccagccgctt ggccaccggc cccaccctca
      661 gctccagggt gtcgccagcg gccaactcca acagctcggg ccgcccagcc tcagccaacc
      721 ccgacggatc agacgggaag gactcggctg gcgtcggtcg gtcgggtcga gtgtgctgct
      781 gcatgatctc ctccatgtcg gcgcgctggc gccgcggggt tctgagcagg gcccgattcg
      841 cctccatgcg gtgcgtttcg tctcctgtgc tgcggttccc tgggatgggg gcaccgtgtc
      901 accgacctac cggcgacgca agaaaagcaa gcggtgccgg ccgctgacct cggcgttggt
      961 acgcaccgca gccttgagcg agctcacgtg gcatacgatg cgccggttcg cggcggcctt
     1021 cggcaccggg ttccgagagg taaaggtcgg cgacgagatc cgcatccacg gccgccccta
     1081 cgaccaccaa cgccacccac caatcgacat cagtgatcta gggcgttgtc catttgtaga
     1141 tgaagccgct cgagccgggg agggtgaagc tggcgaagcg gccgtcggtg ctgtgcaggg
     1201 cggtcgggtc gcgcgggtcg ttggtgccat cgggaggcag gtcgtcgacc cggtcgatgt
     1261 cggccagctt gacccaggcc cccggccggc cgagttccag gtcgacctgt acgtcgaaac
     1321 cctcgaagtt gagcaggaca gccagggtgt cggtgggccg atcgctgggg tgcgcgcgcc
     1381 agccgacggt gcggcggccg ccgccgtggg attcgtccag ccaggggcca aggatccact
     1441 cgaactggcc ggcggcggcc gggtcgaagc cgcgcaggcg caggcccggg tagcggcggc
     1501 gcaggcggac cagccggcgg caccactggt agaagccgtg ctggtcgagg ctggccggcc
     1561 agtcgacggc gaccacgttg cgtggccgtt ccagattgaa ctcctggccc atgtagatca
     1621 tcggctggcc cagggcgacc agggtggcga acatgccgag gcggcccttg cgttccttgg
     1681 ccgccgggtt gtccagccag ggtgtgaact gcagctcgta ggggacgctc tgctcgtcgt
     1741 ggctctcaca gtagttgacg acgttgttgg tgtgggcggc gaagcgggag cggctgaagt
     1801 agaaggtgtc gcccaggttg tcggtgccgg ccgactggcc ctcgaactgg ccctcgcgca
     1861 gcagcgcctt gagcttgtcg tggaacagct ccgaccactg cccgtagccg ttccagcctt
     1921 ccaggttgag gtcgccctgg ttgggcaggt tctcggccac cagcagcaca tcgggcttgc
     1981 cggtcttgag ctccgcggcc aggcggtgca ggaagccgtg gtccatgtag tcggtatggg
     2041 tcgcgtccag ccggaagccg tcgacgtggt actcgccgac cagcatccga caggagtcga
     2101 tgagtaggtt ctggacgtcg gccttctcgg tggcgacccg gttgccccac ggggtggcgc
     2161 cgttgaaata caggccgccg ccggcgtcat cgggctgctc gaggatcagg tcccacagcg
     2221 ggttgaagtc gttgctggtg tggttgaaga ccatatcgac caggagcgcg aggccgcggc
     2281 cgtgggcggc gtcgacgagc cggcggaggt cgtcgggacc gccgaagtcg cgctcgacgg
     2341 cgtggtacag cgacgggtcg tagccgagtg actgggggcc ctgcatgctc ggcacctcgg
     2401 cgagcggcat gagcgacagg gccgtcaccc cgagcgagtc gaagtagccg gtgcggatgc
     2461 gctcggtgat gccgtcgaag cggccctgct ggtcgggccg gatgtcgggg tcgccgtcgg
     2521 tgaagccgtg caccgatacc tcgtagagga tgaggtcggc cgggtcgggc gtgcgccagc
     2581 cgcggtcgct ccaggcgaag gtggtcgggt cgaccaccac ggcgttgctg cgctggtcgc
     2641 cgggccccag ctggcgggcg taggggtcga ccaccagccg gccgccggcg gcgtcctcgg
     2701 gtggggtgcc gccctgcacc cagaaccggt actcgtcgcc ggcgccggcc cggtcggcga
     2761 ccgccagcca ggtgttcgcc gccccgaacc agccccggaa cagctcgagt tcgacgaacg
     2821 cgtcggggtc cggccgctgg tggccgggat gctgccagtc gttgaagctg ccggccacga
     2881 acacgcgggc cgcggtcggg tggtagagcc cgaacagcac ccgcctgtcg gccag
//
LOCUS       DASSWR010000551         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1009665, whole genome shotgun sequence.
ACCESSION   DASSWR010000551 DASSWR010000000
VERSION     DASSWR010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009665"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..642
                     /locus_tag="VFO47_09225"
     CDS             25..642
                     /locus_tag="VFO47_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET8788917.1"
                     /translation="MRKIVANLFISLDGVVEEPGDWHFPYFNDEMGAAVDAGLRSADT
                     LLLGRKTYDDHAGAWPEREEAGDEDAEFAKVLGDMRKIVVSRQRLELAWRNSEQLEGD
                     LVEAVTALKNQPGDGNIAMSGSVSVVRQLLAAGLLDELHLLVHPIAVRKGMRLFDEGE
                     PPVPLRLLSSEAFSTGVLNLVYGPAEPAGDVTYEDAKAHLSQPEQ"
     gene            complement(679..855)
                     /locus_tag="VFO47_09230"
     CDS             complement(679..855)
                     /locus_tag="VFO47_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788918.1"
                     /translation="MHAATATTAGWIEAAADGGPECFFCGDPAKRYKPLNEGTLTSLR
                     KLWWCRPCETTWVA"
     gene            1082..1285
                     /locus_tag="VFO47_09235"
     CDS             1082..1285
                     /locus_tag="VFO47_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HET8788919.1"
                     /translation="MEDQVRVEWRKSTLSTTNGCVEVAVVGDRIAVRDSKDQGQGPVL
                     EFTAVEWDAFLGGVRGGEFDLEP"
     gene            complement(1289..2146)
                     /locus_tag="VFO47_09240"
     CDS             complement(1289..2146)
                     /locus_tag="VFO47_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8788920.1"
                     /translation="MTTRTPTGRRRRLGAELRRLREETGLTIDQVAEVLECSQSKVSR
                     IETGQVSATPRDVRDMLALYRVSDAQREAMVQIAREARQPGWWQKFVDVPDGVPAYVG
                     LETAATSIDIYMSVIVPALLQTADYARAVIGAVRPDLPGAEIDRRVELRLRRQALLDQ
                     DSPPALRVLLDDTVLRRPVGGEKVMAAQRRRLLEDAARPAVTLQVLPVEAGAHAGMDG
                     PFTIFGFPAPAERDVVALDSAADALYLEGAEDVARYRRVFGLLLPAALTPEASAGRIA
                     AAGVAAEAV"
     gene            2295..>2782
                     /locus_tag="VFO47_09245"
     CDS             2295..>2782
                     /locus_tag="VFO47_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8788921.1"
                     /translation="MATTRPRERILDAATRLFADEGINATSVDRVIAEADVAPMTVYR
                     KFGGKDELVAATVEQWSVRWLAWLRDESWTDTGDPGARLEGLWDALEKWFADEGFRGS
                     YVDNVATELRAKPGHPAQAAIAAHRAALRELIHKLATAAGVPSPEGAALQLHVLIDGA
                     TAV"
BASE COUNT          380 a          964 c         1045 g          393 t
ORIGIN      
        1 ggctggtcgt gatggaggag gatgatgcgg aagatcgtcg ccaacctgtt catctcgctc
       61 gacggggtcg tcgaggagcc gggggactgg cacttcccgt acttcaacga cgagatgggc
      121 gcggccgtcg acgctggcct ccgctcggcc gacacgctgc tgctgggccg caagacctac
      181 gacgaccacg ccggggcgtg gccggagcgg gaggaggccg gcgacgagga cgccgagttc
      241 gccaaggtgc tcggcgacat gcgcaagatc gtcgtgtcgc gccagcggct ggagctggcc
      301 tggcggaact ccgagcagct ggagggcgac ctcgtcgagg ccgtgaccgc cctgaagaac
      361 cagccgggcg acgggaacat cgccatgagc gggtcggtct cggtggttcg ccagctgctg
      421 gccgcggggc tgctggacga gctgcacctg ctggtgcacc cgatcgccgt gcgcaagggc
      481 atgcggctgt tcgacgaggg cgagcccccg gtcccgctgc ggctgctctc gtccgaggcg
      541 ttcagcacgg gcgtgctgaa cctcgtctac gggcctgccg agccggccgg cgacgtcacc
      601 tatgaggacg ccaaggcgca cctgtcccag cccgagcagt agccgccagg cgcgggccgg
      661 cgttggggaa gcccgcggtc aggccaccca ggtggtctcg caggggcggc accaccacag
      721 cttcctgagg ctggtgagcg tcccctcgtt cagcggcttg tagcgcttgg ccgggtcccc
      781 gcagaagaag cactccggcc ctccgtcagc cgccgcctcg atccatcccg cggttgtggc
      841 ggtggcggcg tgcattccct ccaatcgcgg cgtggtgttc acgttcctcg ctccgtcgtc
      901 ggccgtccgg cagcggaccg gtgtcgtcca cggccgggat cgagcctagt tgtcacgtgt
      961 tgcgccctgg ttgtcgcttg gtgacgtctg cgttgcgtcg ctggtcgcca caggggttag
     1021 catctgcgac cgacgaccgg ccgccggggc cggcacggac ggggaggggg ctgggcgaca
     1081 ggtggaggac caggtgcggg tcgagtggcg caagagcacc ctcagcacca ccaacgggtg
     1141 cgtcgaggtg gcggttgtcg gcgaccggat cgcggtccgc gactccaagg accagggcca
     1201 gggcccggtg ctggagttca cggcggtcga gtgggacgcc ttcctgggcg gggtccgcgg
     1261 aggcgagttc gacctggagc cctgaccgct agaccgcctc ggccgccacg ccggcggcgg
     1321 cgatgcgccc ggccgacgcc tcgggcgtga gcgccgccgg caggagcagc ccgaacaccc
     1381 gccggtagcg ggccacgtcc tcggcaccct ccaggtagag ggcgtcggcg gcgctgtcga
     1441 gggcgaccac gtcgcgctcg gccggggcgg ggaagccgaa gatggtgaac ggcccgtcca
     1501 tcccggcgtg ggccccggcc tcgacgggca gcacctgaag ggtgaccgcc ggccgggccg
     1561 cgtcctcaag cagccgccgc cgctgggcgg ccatgacctt ctcgccgccg accgggcgcc
     1621 ggagcaccgt gtcgtccagg aggacccgca gcgccggggg gctgtcctgg tcgagcaggg
     1681 cctggcggcg caggcggagc tcgacccgcc ggtcgatctc ggctccgggc aggtcggggc
     1741 ggaccgcgcc gatcaccgcc cgggcgtagt cggcggtctg gagcagggcg ggcacgatca
     1801 ccgacatgta gatgtcgatc gaggtcgcgg ccgtctccag cccgacatag gcgggtacgc
     1861 cgtcgggcac gtcgacgaac ttctgccacc agcccggctg gcgggcctcc ctggcgatct
     1921 ggaccatggc ctcgcgctgg gcgtcggata cccggtacag cgccagcatg tcccggacgt
     1981 cgcgcggggt cgcgctgacc tgccccgtct cgatgcggga gaccttggac tgggagcact
     2041 cgagcacctc ggcgacctgg tcgatggtca gcccggtctc ctcccggagc cggcgcagct
     2101 cggccccgag gcgccgccgg cggccggtcg gggtccgcgt ggtcatctgg gctccccgtg
     2161 gctcaggggc tggctcgagt ggtgcggcaa ggttgtcatg ggcagggatg cattctgcat
     2221 agttgcagag tctgcccggt tcgttcagac tcctgcaaga gagaccattc tctaccttcc
     2281 ggcgggaggc caccatggcg accaccaggc cgcgggagcg gattcttgac gcggcgaccc
     2341 gcctcttcgc cgacgagggc atcaacgcga ccagcgtcga ccgggtcatc gccgaggccg
     2401 acgtcgcccc catgaccgtc taccgcaagt tcggcggcaa ggacgagctg gtggcggcca
     2461 ccgtggaaca gtggagcgtg cgctggctgg cctggctgcg ggacgagtcg tggacggaca
     2521 ccggcgaccc cggagcgcgg ctcgagggcc tctgggacgc gctcgagaag tggttcgccg
     2581 acgagggctt ccgcgggtcg tacgtggaca acgtcgccac cgagctccgg gccaagcccg
     2641 gccaccccgc ccaggcggcc atcgccgccc accgggccgc cctgcgggag ctgatacaca
     2701 aactcgccac cgcggccggc gtgccctcgc ccgagggagc tgccctccag ctccacgttc
     2761 tgatcgacgg tgccactgcg gt
//
LOCUS       DASSWR010000552         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_868080,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000552 DASSWR010000000
VERSION     DASSWR010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868080"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFO47_09250"
     CDS             complement(<1..654)
                     /locus_tag="VFO47_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET8788922.1"
                     /translation="MRALVVEGPGVAAVTDVPEPVPGPGEVVVAVDAAGICGSDVELL
                     DGRRPAAYVSYPVVPGHEWAGRVAAVGPEVAGLAPGDPVVAEGLRSCGICDRCAEGRT
                     NLCGAGYAETGFTLPGALAERLVVPAGLVHRLPADRPLEAAALLEPAACVASGLLETG
                     MPLPGQRVAVVGDGPLGLLALLLLRTASPAELVLVGGRPGRSALGPGCGATRVVAAGD
                     "
     gene            complement(654..1463)
                     /locus_tag="VFO47_09255"
     CDS             complement(654..1463)
                     /locus_tag="VFO47_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788923.1"
                     /translation="MATGGNGEALRVEHVAKRFGGVTALADVNLHLGRGEVLGLIGDN
                     GAGKSTLIKIICGFHQPDAGRIFVGGEEVVLKSVDHARTLGIDTVYQDLALVNELSVY
                     HNMFLNREMVRWPLLSNRRMRRLAREHLEDMGVNIPSVEDEVAKLSGGQRQAIAVARS
                     VYSDARILLLDEPLAAMGAKEGALILDLVRDLKDRGEVSIIIIAHNYGQILEVCDRIN
                     LLQHGTITFDKPTSQTSVQELTDLVVAEYRAARTSRLRPDPSQNARPTPRA"
     gene            complement(1463..2500)
                     /locus_tag="VFO47_09260"
     CDS             complement(1463..2500)
                     /locus_tag="VFO47_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788924.1"
                     /translation="MSDTVDVAERVEPAPAGRPSRSLPRRVGEAFLRRREASVLVVAV
                     ALGVYFASASEAFLTQPNLVNIAQATAPAAIIAAGMVLVLVSGEIDLSVAAVYALAPF
                     NMHYLTDYYGVPILLAIVLALAISALIGFINGFITVFFRVPSFVTTLGTANIVIGIVL
                     VTSHAYPAPIPDAATPTIRAWFGDSDWAHLTWCLLIVAGFHVLLTRTRWGLHTIAVGG
                     NPLGATEAGIRAGRIKIGNFMMVATLGGFAGILDAFRINTIDPNLGGGFGIMFTAVSA
                     AVIGGTSLAGGSGTVIGAFLGALVLAELQDGMNLIGISANPYFLILGVAIVVSMIANV
                     YLSRLRRAGRT"
     gene            complement(2513..3655)
                     /locus_tag="VFO47_09265"
     CDS             complement(2513..3655)
                     /locus_tag="VFO47_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET8788925.1"
                     /translation="MSKVDPVVEQAVDTLGLNRVTRRRLLGGVGLTSASLAASALLAA
                     CTGNDDNQSGDAAAATPGDFPSTPKWKFVFVNHVTTNPFFTPTQYGAQDACALLNCEF
                     QWTGSKDSIVAEMVNATNTAISAKADGIALAVVDKDAFKGPVDQALAAGIPVVSYNAD
                     GAVDDPGTNRLSYIGQGLYDSGFQLGQRAIASGLDSGEVVGFIATPGALNIQPRIDGA
                     SDAIKQSGKSLKFTPVGTNADVTKGLSIIDAYAQGHPDLAGMLAVDAGSTQSVGQVVQ
                     KYSMREKNLKVAGGFDLVPETLNAIKGGSLDYTIDQQPYLQGFLPVLYLYMYKLSGGL
                     VFPSETNTGLLFVTKDNVDPYLQTKTRFEGSSTEQQVVERSGAIAG"
     gene            complement(3687..>4120)
                     /locus_tag="VFO47_09270"
     CDS             complement(3687..>4120)
                     /locus_tag="VFO47_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HET8788926.1"
                     /translation="ETLIPTGEIAPVAGTPLDFTRSTRIGKRIRDNHPQLVIGRGYDH
                     NWVLDRRGSGLELAARLKDPRSGRVLEVLTEEPGIQFYSGNFLDGTLYGTSGRAYRQG
                     DGLALETQHYPDSPNHPNFPSTVLRPGDRYDTTTIYRFSTD"
BASE COUNT          588 a         1388 c         1555 g          589 t
ORIGIN      
        1 gtcgccggcg gccaccaccc gggtggcgcc gcagccgggg ccgagggcgg accggcctgg
       61 ccggccgccc accagcacca gctcggccgg gctggccgtc ctcagcagga gcagcgcgag
      121 caggccgagg gggccgtcgc cgacgaccgc gacccgctgg ccgggcaggg gcatgcccgt
      181 ctccagcagc ccgctggcca cgcaggcggc cggctccagc agggcggccg cctccagcgg
      241 ccggtcggcc ggcaggcggt ggacgaggcc ggcggggacg accagccgct cggccagggc
      301 cccggggagg gtgaagccgg tctcggcgta gccggcgccg cagaggttgg tccggccctc
      361 ggcgcagcgg tcgcagatcc cgcaggaccg cagcccctcg gccaccaccg ggtcgcccgg
      421 ggcgaggccg gccacctccg ggcccacggc cgcgacccgg ccggcccact cgtgcccggg
      481 caccaccggg tagctgacgt aggcggccgg ccgccggccg tcgagcagct cgacgtcgga
      541 gccgcagatc ccggcggcgt cgaccgccac caccacctcg cccggccccg ggacgggctc
      601 gggcacgtcg gtgacggcgg ccaccccggg cccctccacc accagcgcgc gcactaggcc
      661 cgcggcgtcg gccgggcgtt ctgggagggg tcgggccgca gccgggaggt ccgggcggcg
      721 cggtactcgg ccacgaccag gtcggtcagc tcctgcaccg acgtctggga ggtcggcttg
      781 tcgaaggtga tcgtgccgtg ctggagcagg ttgatccggt cgcagacctc gaggatctgg
      841 ccgtagttgt gggcgatgat gatgatcgag acctcgcccc ggtccttgag gtcgcgcacc
      901 aggtcgagga tgagggcgcc ctccttggcc cccatggccg ccagcggctc gtccagcagc
      961 aggatcctgg cgtcgctgta gaccgagcgg gcgaccgcga tcgcctggcg ctggccgccc
     1021 gacagcttgg ccacctcgtc ctcgaccgac gggatgttca cccccatgtc ctccaggtgc
     1081 tcccgggcca gccgccgcat gcggcgattg ctgagcagcg gccagcgcac catctcccgg
     1141 ttgaggaaca tgttgtggta gacgctcagc tcgttgacca gggccaggtc ctggtagacg
     1201 gtgtcgatcc cgagcgtgcg ggcatggtcg acggacttga gcacgacctc ctcgccgccg
     1261 acgaagatcc ggccggcgtc gggctggtgg aagccgcaga tgatcttgat cagggtcgac
     1321 ttgccggcgc cgttgtcgcc gatgaggccg agcacctcgc cccggcccag gtgcaggttg
     1381 acgtcggcca gggccgtgac cccaccgaag cgcttggcca cgtgctcgac ccggagggcc
     1441 tcgccgttgc cgccggtggc catcaggtcc tgcctgcccg gcggagcctg gagaggtaca
     1501 cgttggcgat catcgagacg acgatggcca cgcccaggat caggaagtac gggttggcgc
     1561 tgatgccgat gaggttcatg ccgtcctgca gctcggccag gacgagtgcg cccaggaagg
     1621 ccccgatgac ggtgccggag ccgccggcca gggaggtgcc cccgatcacc gcggccgaga
     1681 cggcggtgaa catgatcccg aagcccccgc cgaggttggg gtcgatcgtg ttgatgcgga
     1741 aggcgtcgag gatcccggcg aacccgccga gggtggccac catcatgaag ttgccgatct
     1801 tgatgcggcc ggcccggatc cccgcctcgg tggccccgag cgggttgccg ccgacggcga
     1861 tcgtgtgcag cccccagcgg gtcctggtca gcagcacgtg gaagccggcc acgatcagca
     1921 ggcaccaggt gaggtgggcc cagtccgagt cgccgaacca ggcccggatg gtcggggtgg
     1981 cggcgtcggg gatcggcgcc gggtaggcgt gcgaggtgac cagcacgatg ccgatgacga
     2041 tgttggcggt gcccagggtg gtcacgaacg acggcaccct gaagaacacg gtgatgaagc
     2101 cgttgatgaa gccgatcagg gccgagatgg ccagggccag gacgatcgcc agcaggatcg
     2161 gcacgccgta gtagtcggtc aggtagtgca tgttgaacgg ggccagcgcg tacaccgccg
     2221 ccaccgacag gtcgatctcg ccgctgacca gcaccagcac catgccagcg gcgatgatcg
     2281 ccgccggcgc ggtcgcctgg gcgatgttga ccaggttggg ctgggtgagg aacgcctccg
     2341 aggccgaggc gaagtagacg ccgagggcga ccgcgaccac caggacgctg gcctcgcgcc
     2401 ggcgcaggaa cgcctcgccg acccgccggg gcaggctgcg ggagggccgg ccggccggag
     2461 ccggctcgac cctctcggcg acgtcgacgg tgtcgctcaa cctctccctc gcctagccgg
     2521 cgatcgctcc ggaacgctcc accacctgct gctcggtgct ggagccctcg aagcgggtct
     2581 tggtctgcag gtaggggtcg acgttgtcct tggtcacgaa cagcaggccg gtgttggtct
     2641 cggacgggaa gaccaggcct cccgagagct tgtacatgta gaggtatagc accggcagga
     2701 acccctgcaa gtacggctgc tggtcgatgg tgtagtcgag gctgccgccc ttgatcgcgt
     2761 tcagcgtctc cggcaccagg tcgaacccgc cggcgacctt caggttcttc tcccgcatcg
     2821 agtacttctg caccacctgg ccgaccgact gggtcgagcc ggcgtcgacc gccagcatgc
     2881 cggccaggtc cgggtggccc tgagcgtagg cgtcgatgat cgacaggccc ttggtcacgt
     2941 cggcgttggt gccgaccggg gtgaacttga gcgacttgcc ggactgcttg atggcgtcgg
     3001 aggcgccgtc gatccggggc tggatgttga gcgcgcccgg ggtggcgatg aagccgacca
     3061 cctcgccgga gtccaggccc gaggcgatcg cccgctggcc gagctggaac ccggagtcgt
     3121 agaggccctg gccgatgtag gagaggcggt tggtgccggg gtcgtccacc gcgccgtcgg
     3181 cgttgtagga gacgaccggg atgccggcgg ccagcgcctg gtcgaccggg cccttgaagg
     3241 cgtccttgtc gaccaccgcc agcgcgatcc cgtcggcctt ggccgagatg gcggtgttgg
     3301 tggcgttcac catctcggcc acgatcgagt ccttggaccc cgtccactgg aactcgcagt
     3361 tgagcagggc gcaggcgtcc tgggcgccgt actgggtggg cgtgaagaac gggttggtgg
     3421 tgacgtggtt gacgaacacg aacttccact tcggggtcga ggggaagtcg ccgggcgtcg
     3481 cggccgcggc gtcgccgctc tggttgtcgt cgttgccggt acaggcggcc agcagcgccg
     3541 aggcggccag cgacgcgctg gtcaggccga cgccacccag cagccggcgc cgggtgacgc
     3601 ggttcagccc cagggtgtcg acggcctgct cgacgacggg atcgaccttg gacattgcaa
     3661 ccctcctcgg gcgggtcagg gcccgttcag tcagtggaga agcggtagat ggtggtggtg
     3721 tcgtaccggt caccgggccg cagcacggtc gacgggaagt tcgggtggtt gggcgagtcg
     3781 gggtagtgct gggtctcgag ggcgaggccg tcgccctggc ggtaggcccg gccgctggtg
     3841 ccgtacagcg tcccgtcaag gaagttgccg gagtagaact ggatcccggg ctcctcggtg
     3901 aggacctcca gcacccgccc gctgcgcggg tccttgagcc gcgccgccag ctccaggccg
     3961 gagccgcgcc ggtccagcac ccagttgtgg tcgtagccgc ggccgatgac gagctgcggg
     4021 tggttgtcgc ggatgcgctt gccgatcctg gtcgagcggg tgaagtccag cggcgtgccg
     4081 gcgaccgggg cgatctcccc ggtggggatc agggtctcgt
//
LOCUS       DASSWR010000553         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_303115,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000553 DASSWR010000000
VERSION     DASSWR010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303115"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..157
                     /locus_tag="VFO47_09275"
                     /pseudo
     CDS             86..157
                     /locus_tag="VFO47_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(196..504)
                     /locus_tag="VFO47_09280"
     CDS             complement(196..504)
                     /locus_tag="VFO47_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788927.1"
                     /translation="MTLDELRRIAEARGIATRFTDAANQTYEVSQATLEAVLDAMGPP
                     PGRAVWPPVVVARSGQRAGWSPPAGEPVTLVLEGGQERPVPVGLPGDLPPGWHRLEGL
                     "
     gene            555..>2458
                     /gene="glgX"
                     /locus_tag="VFO47_09285"
     CDS             555..>2458
                     /gene="glgX"
                     /locus_tag="VFO47_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET8788928.1"
                     /translation="MTAAPEPVWPGQPRPLGATWDGEGTNFAVFAEGADAVDLALFDQ
                     TGVERRLRLPEVTAHTWHGYVPEVGPGQRYGFRVHGAYDPERGERRNPDKLLLDPYAR
                     AIEGVVDWDDSVFGYRVGGDDLHRDERDSSPHVPRSVVVHDPFPWGDDRRPGVPWADT
                     IIYEAHVRGFTMRHPDVPPHQRGSYAAMGSDPVVAHLQRLGVTTLELLPVHQFVSEHH
                     LVSMGLTNYWGYNSIGFFAPEARYSSTGTRGQQVSEFKAMVRNLHAAGIEVILDVVYN
                     HTAEGNHMGPTLSFRGLDNNSYYRTVDSNRRFYMDYTGTGNTLNARHPQVLALIMDSL
                     RYWVEEMHVDGFRFDLASALARSFHEVDRLSAFFDLIHQDPVVSRVKLIAEPWDVGEG
                     GYQVGNFPVLWTEWNGRYRDTMRDFWRGARTGVADIAYRLTGSSDLYQSDGRRPYASI
                     NFVTCHDGFTLNDLVSYNDKHNEANGEGNRDGTSDNRSWNCGEEGPADDLEVRHLRER
                     QKRNLLASLLLSAGVPMLLAGDEFSRTQQGNNNAYCQDNEISWVDWELGQDQEALVEF
                     TRAVIALRRDHPVFRRRKFFQGQAIHGSGVKDIGWFTPDGAEMDETHWRAPDISTLGV
                     FLNGEGLPDRG"
BASE COUNT          366 a          924 c          845 g          323 t
ORIGIN      
        1 cctgccgcag gcgctggacg acgggggccg tgcctggggc tgggccgccc agctctacgc
       61 cgtccgctcg cgccgcagct ggggcatggg cgacctcggc gacctggccg ggctgctggc
      121 ggccacggcg cccttgggcg ccgggttcgc gctgctcaat cccttccacg ccgccctgcc
      181 gggtgagccg agcccctaca acccctccag ccggtgccag cccgggggca ggtcgccggg
      241 cagcccgacc gggaccggcc gctcctggcc gccctccagc accagcgtga ccggctcgcc
      301 cgccgggggg ctccagccgg cccgctggcc gctgcgggcg accaccaccg ggggccagac
      361 cgcccggccc ggcggggggc ccatggcgtc gagcacggcc tcgagggtgg cctgcgagac
      421 ctcgtacgtc tggttggcgg cgtcggtgaa ccgggtggcg attccgcgtg cttcggcgat
      481 tcggcggagc tcgtcgagcg tcatgtccgg aggttacggc acactgaccc gaccatgacc
      541 acctgccgcg ggcgatgacg gccgctccag agcccgtgtg gccggggcag ccccgtccgc
      601 tgggcgcgac atgggacggc gagggcacca acttcgccgt cttcgccgag ggggccgacg
      661 ccgtcgacct ggccctgttc gaccagaccg gggtcgagcg gcgcctgcgc ctgcccgagg
      721 tcaccgccca cacctggcac ggctacgtgc ccgaggtcgg ccccggccag cgctacggct
      781 tccgggtcca cggcgcctac gacccggagc ggggggagcg ccgcaacccg gacaagctgc
      841 tgctcgaccc ctacgcccgg gccatcgagg gggtggtcga ctgggacgac tcggtgttcg
      901 gctaccgggt cggcggcgac gacctgcacc gcgacgagcg cgacagcagc ccgcacgtgc
      961 ccaggtcggt ggtggtccac gacccgttcc catgggggga cgaccgccgc ccaggagtgc
     1021 cctgggcgga cacgatcatc tacgaggccc acgtccgcgg gttcaccatg cgccaccccg
     1081 acgtcccacc gcaccagcgc ggcagctacg ccgccatggg ctcggacccg gtcgtcgccc
     1141 acctccagcg tctcggggtg acgaccctgg agctgctccc ggtccaccag ttcgtgtccg
     1201 agcaccacct ggtcagcatg gggctgacca actactgggg ctacaactcg atcggcttct
     1261 tcgcccccga ggcccgctac tcgtccaccg gcacccgcgg ccagcaggtg agcgagttca
     1321 aggcgatggt ccgcaacctg cacgcggccg ggatcgaggt gatcctcgac gtcgtctaca
     1381 accacaccgc cgagggcaac cacatgggcc cgacgctgtc gttccggggc ctggacaaca
     1441 actcctacta ccggacggtg gactccaacc ggcgcttcta catggactac accggcaccg
     1501 gcaacacctt gaacgcccgc catccccagg tgctggccct gatcatggac tcgctgcgct
     1561 actgggtcga ggagatgcac gtcgacgggt tccgcttcga cctggccagc gccctggccc
     1621 gcagcttcca cgaggtcgac cgcctgtcgg ccttcttcga cctgatccac caggacccgg
     1681 tggtgagccg ggtcaagctg atcgccgagc cctgggacgt gggcgagggc ggctaccagg
     1741 tgggcaactt cccggtgctg tggaccgagt ggaacggccg ctaccgcgac accatgcgcg
     1801 acttctggcg cggcgcccgc accggggtgg ccgacatcgc ctaccggctc accggctcca
     1861 gcgacctgta ccagtccgac ggccgccggc cgtacgccag catcaacttc gtcacctgcc
     1921 acgacggctt caccctgaac gacctggtca gctacaacga caagcacaac gaggccaacg
     1981 gcgagggcaa ccgggacggc accagcgaca accgctcctg gaactgcggc gaggagggcc
     2041 cggccgacga cctggaggtc cggcacctgc gcgagcgcca gaagcggaac ctcctggcca
     2101 gcctgctgct gtcggccggg gtgccgatgc tgctggccgg cgacgagttc agccgcaccc
     2161 agcagggcaa caacaacgcc tactgccagg acaacgagat ctcctgggtc gactgggagc
     2221 tgggccagga ccaggaggcg ctggtcgagt tcacccgggc ggtgatcgcc ctgcgccgcg
     2281 accacccggt gttccggcgg cgcaagttct tccagggcca ggccatccac ggctccgggg
     2341 tgaaggacat cggctggttc accccggacg gggccgagat ggacgagacc cactggcggg
     2401 cccccgacat ctccaccctg ggggtgttcc tcaacggcga ggggctgccc gaccgggg
//
LOCUS       DASSWR010000554         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1348947, whole genome shotgun sequence.
ACCESSION   DASSWR010000554 DASSWR010000000
VERSION     DASSWR010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348947"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VFO47_09290"
     CDS             complement(<1..1433)
                     /locus_tag="VFO47_09290"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8788929.1"
                     /translation="MSKLRGRNGSPHVVVVGGGFAGLAVVKTLAKVTPPVRVTLLEQH
                     NYHLFQPLLYQLATGVVQPADIAHPVRGIVRRYRRTSVRMGTASGVDLDARQVLTEEG
                     GRIGYDYLVLAAGATTATFGIPGVEEHSFPLKTMPDALRLRAHLLHQFELAETDPAMI
                     DKGALTVVVVGGGPTGVEMAGALHELFKHVLVHDFPELDINQSRVVLLEATDHLLAPF
                     HPSSRRHALDILEKRGVEVRLGQAMSNATPDEVVLKDGTVIPTRTLIWGAGVRANPLA
                     DVLGLEQTRGGRILVGDLAGAGDGKGGLLPQVAQPAIQEGKHAALQIGRTLNGEPRTG
                     FEYKDKGIMATIGRNAAVTELPNGARFRGVLAWYMWLALHLAYIIGFRSRIAVLVNWI
                     WSYLTYDRHARIIVAVEPSRRALPGQPPKPASPEAAPSERPAVRERLTLWFGGRGWVA
                     PIPAAPCRGGRRPWRPRRPWPRPGPGRL"
     gene            1514..1951
                     /locus_tag="VFO47_09295"
     CDS             1514..1951
                     /locus_tag="VFO47_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788930.1"
                     /translation="MTGRADESFGALAQAWSDSLQRLLTDQAESGRAALESLSSTLAA
                     TEEALASQEETNRALRKSLAAFREVIEQAGATQERSTRLIQAALESFAATTQAQLELT
                     KAFMAPMGAQPEAFGNLVQEWNDAFLRLLEAAPGVQPRRPEPE"
     gene            1972..2874
                     /locus_tag="VFO47_09300"
     CDS             1972..2874
                     /locus_tag="VFO47_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET8788931.1"
                     /translation="MEWVAGGRFLMGSEDFYPEERPVHEVAVGGFWVDRHPVTNAQFR
                     RFVKATGYVTVAEQPPDPADYPDADPDLLVPGALVFQPTTGPVDLRDWRNWWAWVPGA
                     SWRHPEGPGSTLHGRDLHPVVQVCHADAQAYADWAGKALPSEAEWEYAARGGLEGKVY
                     TWGDEPTPKGRQMANTWQGEFPWRNLLLDRYERTSPVGSFPPNGYGLSDMAGNVWEWT
                     SDRFTASHDNDACCAPHDPRETIPRNVIKGGSHLCAPSYCLRYRPAARQGEAVDTATS
                     HIGFRCILRSAPGGPGDQAGGGKG"
     gene            complement(2827..>3283)
                     /locus_tag="VFO47_09305"
     CDS             complement(2827..>3283)
                     /locus_tag="VFO47_09305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET8788932.1"
                     /translation="AALPATRRWFMDERGTTGGPGYALYHTLVRIPFGTAVTEELLFR
                     GVLCGLLLQRHSRVRAAAVSSALFGCWHVLPTLDTIGLNRAGAAVRDDPARTTAAVAA
                     SVAVTAVAGLGFSWLRFRADSVLAPAVVHAALNSSAFAAARLVARAARR"
BASE COUNT          464 a         1201 c         1215 g          403 t
ORIGIN      
        1 aggcgaccgg gcccgggccg cggccatgga cgccgcggcc gccacggccg ccggccgcct
       61 cggcatggag ctgccgggat gggggcgacc cagcctcggc ccccgaacca caacgtgagg
      121 cgctcgcgca ccgccggccg ctcgctgggc gccgcctcgg gggacgccgg cttcggcggc
      181 tgaccgggga gcgcccggcg cgacggctcc acggcgacga tgatgcgggc gtggcggtcg
      241 taggtgaggt agctccagat ccagttgacc agcacggcga tccggctgcg gaaaccgatg
      301 atgtaggcca ggtgcaacgc cagccacatg taccaggcca gcacaccccg gaacctggcc
      361 ccgttgggca gctcggtcac ggcggcgttg cggccgatgg tggccatgat ccccttgtcc
      421 ttgtactcga agccggtccg gggctcgccg ttgagggtcc ggccgatctg gagggcggcg
      481 tgcttgccct cctggatggc cggctgggcg acctggggca gcagcccgcc cttgccgtcg
      541 cccgccccgg ccaggtcgcc gaccaggatc cggccgccgc gggtctgctc cagcccgaga
      601 acgtcggcca gcgggttggc ccgcaccccg gccccccaga tcagcgtcct ggtcgggatc
      661 acggtgccgt ccttgagcac gacctcgtcg ggggtggcgt tggacatggc ctgcccgagc
      721 cgcacctcca ccccgcgctt ctccaggatg tcgagggcgt gcctgcggga gctggggtgg
      781 aagggcgcca gcaggtggtc ggtggcctcc agcagcacca ccctggactg gttgatgtcc
      841 agctcgggga agtcgtgcac gagcacgtgc ttgaacagct cgtggagggc gccggccatc
      901 tccaccccgg tggggccgcc gccgaccacc accacggtga gggcgccctt gtcgatcatg
      961 gccggatcgg tctcggccag ctcgaactgg tgcagcaggt gcgcccggag ccgcagggcg
     1021 tcgggcatgg tcttgagcgg gaagctgtgc tcctcgaccc ccgggatccc gaaggtggcc
     1081 gtggtcgcgc cggcggccag gaccaggtag tcgtagccga tgcggccgcc ctcctcggtc
     1141 aggacctgcc tggcgtcgag gtccaccccc gacgccgtgc ccatccgcac cgaggtgcgg
     1201 cggtagcggc gtacgatgcc ccgcaccggg tgggcgatgt cggccggctg gaccacgccg
     1261 gtggccagct ggtacagcag cggctggaac aggtggtagt tgtgctgctc caggagggtg
     1321 acccgcaccg ggggcgtgac cttggcgagg gtcttgacca ccgccagccc ggcgaacccg
     1381 ccgccgacga cgacgacgtg gggcgacccg ttccggcccc tgagcttcga catcgctccg
     1441 ccttcctccg cctgatcgtt caatcccgag tatgcccggt atggtcgtgg ccggaacaag
     1501 tgggaggtga cccatgaccg gaagagcgga cgagagcttc ggcgccctcg cccaggcgtg
     1561 gtccgactcc ctccagcggc tgctgaccga ccaggcggag agcggccggg ccgccctgga
     1621 gtcgctgtcg tccacactgg ccgcgaccga ggaggccctg gccagccagg aggagaccaa
     1681 ccgggccctg cgcaagagcc tggccgcctt ccgggaggtg atcgagcagg ccggcgccac
     1741 ccaggagcgc agcacccggc tcatccaggc cgccctggag agcttcgccg ccaccaccca
     1801 ggcgcagctg gagctcacca aggccttcat ggcgcccatg ggcgcccagc cggaggcctt
     1861 cggcaacctg gtccaggaat ggaacgacgc cttcctgcgg ctgctggagg ccgcccccgg
     1921 cgtccagccg aggcggccgg agccggaatg agcccggctg cgccgaagga catggagtgg
     1981 gtggccggcg gccgcttcct gatgggctcc gaggatttct atcccgagga gcggcccgtc
     2041 cacgaagtgg cggtcggcgg gttctgggtc gaccgccacc cggtcaccaa cgcccagttc
     2101 cggcgcttcg tcaaggccac cgggtacgtg accgtggccg agcagccccc cgacccggcc
     2161 gactaccccg acgccgaccc cgacctgctg gtccccggcg ccctggtctt ccagcccacc
     2221 accgggccgg tcgacctgcg cgactggcga aactggtggg cgtgggtgcc gggggcgagc
     2281 tggcgccatc cggaggggcc ggggtcgacc ctccacggcc gcgacctcca ccccgtggtg
     2341 caggtctgcc acgccgacgc ccaggcgtac gccgactggg ccggcaaggc gctgcccagc
     2401 gaggccgagt gggagtacgc ggcccgcggc gggctcgagg gcaaggtgta cacctggggg
     2461 gacgagccca cccccaaggg ccgccagatg gccaacacct ggcagggcga gttcccctgg
     2521 cggaacctgc tgctcgaccg gtacgagcgg acctcgccgg tggggtcgtt cccgcccaac
     2581 ggctacggcc tgtccgacat ggccggcaac gtctgggagt ggaccagcga ccgcttcacg
     2641 gccagccacg acaacgacgc ctgctgcgcc ccgcacgacc cccgggagac gatcccccgc
     2701 aacgtcatca agggcggcag ccacctctgc gcccccagct actgcctgcg ctaccggccg
     2761 gcggcccgcc agggggaggc ggtcgacacc gccaccagcc acatcggctt ccgctgcatc
     2821 ctgcggtcag cgccgggcgg cccgggcgac caggcgggcg gcggcaaagg ctgagctgtt
     2881 cagggcggcg tggaccaccg ccggggcgag cacgctgtcg gcccggaagc gcagccagga
     2941 gaagccgagg ccggccacgg cggtgacggc gaccgaggcg gccacggcgg cggtggtgcg
     3001 ggccgggtcg tcgcggacgg ccgcgccggc gcggttgagc ccgatggtgt ccagggtcgg
     3061 cagcacgtgc cagcagccga acagggccga gctcacggcg gcggcccgca cccgggagtg
     3121 gcgctggagg agcaggccgc acaggacccc ccggaacagc agctcctcgg tcacggccgt
     3181 gccgaagggg atccggacca gggtgtggta cagcgcgtag ccgggacccc cggtcgtccc
     3241 ccgctcgtcc atgaaccagc gccgggttgc cggcagggcg gcg
//
LOCUS       DASSWR010000555         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_924484,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000555 DASSWR010000000
VERSION     DASSWR010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924484"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFO47_09310"
     CDS             <1..992
                     /locus_tag="VFO47_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788933.1"
                     /translation="GALAQSVGVTYDAADAARFEQLWKGYTDHLAAYAEGAATGNDAA
                     VQEVRSALLDHCDEWGAWLAEASGGRVEAGQATKNAQARVEQLMEQVDAYADRDYEQA
                     YKLERQAYQSTYGAGTTLASAGLTAKEAADVAKAPENLRSAFAMLLGEHMELIVDAQR
                     ATFAGSPEFEAAAGQVNANTATLAKAMSGIVGPEKAAEFQTAWANHVEGLMAYTAAVA
                     GKDEAAKTVARENLDGFAERLALYFSDVVRNPLATDPLTDAITAHDEHLINQVDAYAA
                     KDYARAQQVQDNGYQQMVGVANVLVDAIEKVMAAGMPAGGSKTGGGGTAHVHP"
     gene            1008..1667
                     /locus_tag="VFO47_09315"
     CDS             1008..1667
                     /locus_tag="VFO47_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET8788934.1"
                     /translation="MTRGRAWPGAAVGAVVLLALGVALAGCTGPRARYAGVSSTVSTE
                     AQSSPEEGAAAVRDFRSVRGYRPTPVPVRLEIARIGVDTALQRLGQDRGGAVEVPTGP
                     RQWEDAGWYAGEGGTRPGDPGSAVILGHVDSKRGPAVFYRLRELRAGDPVVVVRADGS
                     RVRFVVERVERYPKRRFPTEDVYYPTLSPKLRLVTCGGAFDPAANHYRDNVIAFASLS
                     G"
     gene            1692..2897
                     /locus_tag="VFO47_09320"
     CDS             1692..2897
                     /locus_tag="VFO47_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET8788935.1"
                     /translation="MRAEEVDGAGSPTATGAWYRPMVARATDEEHRSATVLELFFDLC
                     FVVAVASAASALHHEVAEDHVRDGVVGYTSVFFAIWWAWMNFTWFASAYDTDDVAYRL
                     TTLVQIAGALILAAGVPDAMDGADFTAITIGYVVMRLAMVTQWLRAAAADPPHRRSAL
                     RFAAGIVAVQVGWVLRLFLPEGPGYASFLVLAAAELAIPIWAERAAPTTWHPRHIAER
                     YGLFTLIVLGECVLASTLAIQAALEEDAVLADLATTAVGGLLTVFGMWWLYFAKESHR
                     FLTSLRAGIVWGYGHYLIFASAAAVGAGLAVNVDYLTHQAAIGPRAAAAAFTVPVALF
                     LVAVWALQIRPLQLHHWHTSLVPTTAVLALVSTLGPEPILVTGLLVAAMIGLSLVALH
                     RRPAVTGAP"
     gene            complement(2899..>3400)
                     /locus_tag="VFO47_09325"
     CDS             complement(2899..>3400)
                     /locus_tag="VFO47_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET8788936.1"
                     /translation="TYVAARLAIDTWRWAGVPFYIRAGKHLPVTDTEVLVEFRRPPLE
                     LFGELIPARSNHLRLHLGPDVRIELGLRVKVPGDHLIGEDVELVAAHHPALGRPPYER
                     LLGDAMEGDTELFAREDAVEAEWQVVDPVLGHDATPLYEYEPGTWGPVEADQLIAGDG
                     TWAVPS"
BASE COUNT          446 a         1256 c         1250 g          448 t
ORIGIN      
        1 ccggcgccct ggcccagtcg gtcggggtga cctacgacgc cgccgacgcc gccaggttcg
       61 agcagctctg gaagggctac accgaccacc tggccgccta cgccgagggc gcggccaccg
      121 ggaacgacgc cgccgtccag gaggtgcggt cggccctgct cgaccactgc gacgagtggg
      181 gcgcgtggct ggccgaggcc agcggcggcc gggtcgaggc cggccaggcc accaagaacg
      241 cccaggcgcg ggtcgagcag ctgatggagc aggtcgacgc ctacgccgac cgggactacg
      301 agcaggccta caagctggag cgccaggcct accagagcac ctacggcgcc gggaccacgc
      361 tggccagcgc cggcctgacc gccaaggagg cggccgacgt ggccaaggcc ccggagaacc
      421 tgcggtcggc cttcgccatg ctgctgggcg agcacatgga gctgatcgtg gacgcccagc
      481 gggccacctt cgccgggtcg cccgagttcg aggccgcggc cggccaggtc aacgccaaca
      541 ccgccaccct ggccaaggcg atgagcggga tcgtcggccc ggagaaggcg gccgagttcc
      601 agaccgcctg ggccaaccac gtcgaggggc tgatggccta cacggccgcg gtcgccggca
      661 aggacgaggc cgccaagacg gtggccaggg agaaccttga cgggttcgcc gagcggctgg
      721 ccctctactt cagcgacgtg gtccgcaacc cgctggccac cgacccgctc accgacgcca
      781 tcaccgccca cgacgagcac ctgatcaacc aggtcgacgc ctacgcggcc aaggactacg
      841 ctcgggccca gcaggtccag gacaacggct accagcagat ggtcggggtg gccaacgtcc
      901 tggtcgacgc catcgagaag gtgatggcgg ccggcatgcc ggccggcggc tccaagaccg
      961 gcggcggcgg gaccgcccac gtccacccgt gaggtgagcg ccaggagatg accaggggca
     1021 gggcctggcc gggagccgcg gtgggtgccg tggtcctgct cgccctgggt gtggcgctgg
     1081 ccggctgcac cgggccgcgg gcccgctacg ccggggtcag cagcacggtg tcgaccgaag
     1141 cccagtcctc gcccgaggaa ggggcggcgg ccgtccgcga cttccgctcg gtccgcggct
     1201 accggcccac gccggtcccg gtgcggctgg agatcgccag gatcggcgtg gacacggcgc
     1261 tgcagcggct cggccaggac cgcggcgggg ccgtcgaggt gccgaccggg ccccgccagt
     1321 gggaggacgc cggctggtac gcgggcgagg gcggcacccg gccgggcgac cccggctcgg
     1381 cggtcatcct cggccacgtc gactccaagc gggggccggc ggtgttctac cggttgcgcg
     1441 agctgcgggc cggcgacccg gtggtggtcg tccgggccga cggctcgcgg gtccggttcg
     1501 tggtcgagcg ggtcgagcgg tacccgaagc ggcgcttccc caccgaggac gtctactacc
     1561 cgacgctgag cccgaagctg cggctggtca cctgcggcgg cgccttcgac ccggccgcca
     1621 accactaccg cgacaacgtg atcgcgttcg ccagcctcag cggctgagcg accaggccgt
     1681 atcatcggcc gatgcgagcg gaagaggtgg acggggccgg gtccccgacg gcgaccggtg
     1741 cgtggtaccg gccgatggtg gcgcgggcga ccgacgagga gcaccgctcg gcgaccgtgc
     1801 tggagctgtt cttcgacctg tgcttcgtgg tcgccgtggc ctcggccgcg tccgcgctcc
     1861 accacgaggt ggccgaggac cacgtccgcg acggcgtggt cggctacacc tcggtgttct
     1921 tcgccatctg gtgggcgtgg atgaacttca cctggttcgc ctcggcctac gacaccgacg
     1981 acgtcgccta ccggctcacc accctggtcc agatcgccgg ggccctgatc ctggccgccg
     2041 gggtgcccga cgccatggac ggcgccgact tcaccgccat cacgatcggc tacgtggtca
     2101 tgcgcctggc catggtcacc cagtggctgc gggccgccgc cgccgacccg ccgcaccggc
     2161 gcagcgcgct gcgcttcgcc gccggcatcg tggccgtgca ggtcggctgg gtgctgcgcc
     2221 tgttcctgcc cgagggaccg ggctacgcca gcttcctggt gctggccgcg gccgagctgg
     2281 ccatccccat ctgggccgag cgggccgctc ccaccacctg gcacccccgc cacatcgccg
     2341 agcgctacgg cctgttcacc ctgatcgtgc tgggcgagtg cgtgctcgcc tccaccctgg
     2401 ccatccaggc ggcgctggag gaggacgccg tcctggccga cctggcgacg acggcggtcg
     2461 gcgggctgct gaccgtgttc ggcatgtggt ggctgtactt cgccaaggag tcccacaggt
     2521 tcctgacctc gctgcgggcc gggatcgtct gggggtacgg ccactacctg atcttcgcct
     2581 cggccgcggc cgtcggggcc ggcctggccg tcaacgtcga ctacctgacc caccaggcgg
     2641 ccatcggccc ccgggcggcg gccgccgcct tcaccgtccc ggtggccctg ttcctggtgg
     2701 ccgtctgggc gctccagatc cgccccctcc agctgcacca ctggcacacc tcgctcgtgc
     2761 ccaccaccgc cgtgctggcc ctggtctcga ccctcggccc ggagcccatc ctggtcaccg
     2821 gcctgctggt ggcggccatg atcgggctgt ccctggtcgc cctgcatcgc cggccggcgg
     2881 tcaccggagc gccctagctc agctggggac cgcccaggtg ccgtcgccgg cgatcagctg
     2941 gtcggcctcc acggggcccc aggtgcccgg ctcgtactcg tacagcgggg tggcgtcgtg
     3001 gcccagcacc gggtcgacca cctgccactc ggcctccacg gcgtcctcgc gggcgaacag
     3061 ctcggtgtcg ccctccatgg cgtcgcccag caggcgctcg tagggtggcc ggccgagggc
     3121 ggggtggtgg gcggcgacca gctccacgtc ctcgccgatc aggtggtcgc ccgggacctt
     3181 gacccgcagg ccgagctcga tgcggacgtc ggggccgagg tggaggcgca ggtggttgga
     3241 gcgggccggg atcagctctc cgaacagctc caggggcggg cgccggaact cgaccaggac
     3301 ctcggtgtcg gtcaccggca ggtgcttgcc ggcgcggata tagaagggca ccccggccca
     3361 gcgccaggtg tcgatggcca ggcgggcggc cacgtaggtc
//
LOCUS       DASSWR010000556         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1236535, whole genome shotgun sequence.
ACCESSION   DASSWR010000556 DASSWR010000000
VERSION     DASSWR010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236535"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFO47_09330"
     CDS             <1..802
                     /locus_tag="VFO47_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET8788937.1"
                     /translation="LAERKELLATATYRVGPFLLPEIKYFMPLLLAWAASLFVLFFEK
                     DLGTSLLFFGVFVVLLYVATGRFSYVAAGLGLFVTGAVIAYNLFGHVQTRVATWLDVW
                     EYYDGRGYQLAQGLFALATGGILGQGWNQGRPDFIPFASTDFIFAAFGEELGLLGVTA
                     MLLVYLLMVFRGLRISLAAPDDFGKLLAVGLVTSFALQVFVIVGGVTRLIPLTGITLP
                     FVSFGGSSLLANYALIALLCRISDEGTPAPAGGHPATPADTGEVTVVR"
     gene            799..2262
                     /locus_tag="VFO47_09335"
     CDS             799..2262
                     /locus_tag="VFO47_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET8788938.1"
                     /translation="MNRQIRRVAVVVLVAFMALLAAPLYWQVLAADRLANDSRNTRVL
                     IKEYSIERGPIVLADQTPVAESRRSRQRGDPLEFVRVYPNGPRYGMVTGFYSLVFGRT
                     LAEQEFNSFLLGRAPEQFGQNVTDLLTARETPGGTLVLTLDKATQAAAEGALGGRKGA
                     VVALDPRTGAVLAMTTYPRYDPNQLSSHNPEAIRRNWERLVNDENGPLLNRAAGALYP
                     PGSTFKVVTAAAALENGVTMDQELPSPPVYDVPQTSADIRNFGGASCRDGSLTLTEAL
                     EISCNTTFAALGVELGSEKLADEAEKFGFNKPSPYQLPAATSVIPRDQDVPATAQSAI
                     GQRDVRVSPLQMASVAATIANGGRRMGPFVARQVVSDRGRVVKRFEGEDFGEVIPEDV
                     AANLRQMMRGVVANGTGQAARIPGEEVAGKTGTAQHARGRNPHAWFIGFAGSGDRQIA
                     VAVVVEEGGDAGSEATGGRTAAPIARDVMAAYMRGQG"
BASE COUNT          333 a          887 c          850 g          351 t
ORIGIN      
        1 cctggccgaa cgcaaggagc tgctggccac cgccacctac cgggtggggc cgttcctgct
       61 gccggagatc aagtacttca tgcccctgct gctggcctgg gcggccagcc tgttcgtgct
      121 cttcttcgag aaggacctcg gtaccagcct gctgttcttc ggggtgttcg tggtcctgct
      181 gtacgtggcc acggggcgct tctcctatgt ggccgcgggg ctgggcctgt tcgtcaccgg
      241 cgcggtgatc gcctacaacc tgttcggcca cgtccagacc cgagtcgcga cctggctgga
      301 cgtctgggag tactacgacg gccgcggcta ccagctcgcc caggggctgt tcgccctggc
      361 caccgggggg atcctcggcc agggctggaa ccagggccgg cccgacttca tcccgttcgc
      421 ctccaccgac ttcatcttcg ccgccttcgg ggaggagctg ggcctgctcg gggtcacggc
      481 catgctgctc gtctacctgc tgatggtgtt ccgggggctg cggatctccc tggccgcccc
      541 cgacgacttc ggcaagctgc tggccgtcgg cctggtgacc agcttcgccc tgcaggtgtt
      601 cgtgatcgtc ggcggggtca cccggctgat cccgctcacc ggcatcaccc tgccgttcgt
      661 gagcttcggc gggtcgagcc tgctggccaa ctacgccctg atcgcgctcc tgtgccggat
      721 ctcggacgag ggcaccccgg ccccggccgg cggccacccg gccactccgg ccgacaccgg
      781 ggaggtgacg gtcgtccgat gaaccggcag atccggcggg tggccgtagt cgtcctggtc
      841 gccttcatgg ccctgctcgc cgcccccctc tactggcagg tgctggccgc cgaccggctg
      901 gccaacgact cccgcaacac ccgggtgctg atcaaggagt actcgatcga gcgggggccg
      961 atcgtgctcg ccgaccagac cccggtggcc gagtccaggc gctcccgcca gcgcggcgac
     1021 ccgctcgagt tcgtccgcgt ctacccgaac ggcccccgct acggcatggt caccggcttc
     1081 tactcgctgg tctttggccg caccctggcc gagcaggaat tcaactcgtt cctgctgggg
     1141 cgggcgcccg agcagttcgg ccagaacgtg accgacctgc tcaccgcccg cgagaccccc
     1201 ggcggcacgc tcgtcctgac cctggacaag gccacccagg cggcggccga gggcgcgctg
     1261 ggcgggcgca agggggcggt ggtggcgctc gaccccagga ccggggcggt gctggccatg
     1321 accacctacc cccgctacga ccccaaccag ctgtccagcc acaaccccga ggccatccgg
     1381 cgcaactggg agcggctggt caacgacgag aacgggccgc tgctgaaccg ggccgccggg
     1441 gcgctgtacc cgcccgggtc gaccttcaag gtggtcacgg cggcggcggc gctggagaac
     1501 ggcgtcacca tggaccagga gctgccctcc ccgccggtct acgacgtgcc ccagacctcg
     1561 gccgacatcc gcaacttcgg cggtgcgagc tgccgcgacg gcagcctgac cctgaccgag
     1621 gcgctggaga tctcctgcaa caccaccttc gccgccctcg gggtcgagct gggcagcgag
     1681 aagctggccg acgaggcgga gaagttcggc ttcaacaagc ccagccccta ccagctcccg
     1741 gcggccacca gcgtcatccc gcgcgaccag gacgtgccgg ccaccgccca gagcgccatc
     1801 ggccagcgcg acgtgcgcgt ctcgccgctg cagatggcca gcgtggccgc gaccatcgcc
     1861 aacggcgggc ggcggatggg gccgttcgtg gcccgccagg tcgtctccga caggggccgg
     1921 gtggtcaagc ggttcgaggg cgaggacttc ggcgaggtga tccccgagga cgtggccgcc
     1981 aacctccgcc agatgatgcg cggggtggtc gcgaacggca ccggccaggc cgcccggatc
     2041 cccggggagg aggtggccgg caagaccggt accgcccagc acgcccgcgg ccggaacccc
     2101 cacgcctggt tcatcggctt cgccgggtcg ggcgaccggc agatcgccgt cgccgtggtc
     2161 gtcgaggagg gcggcgacgc cggcagcgag gccaccggcg gccgcaccgc cgcccctatc
     2221 gcccgggacg tcatggccgc ctacatgaga ggacaagggt gatcgcggag ggggttcccc
     2281 aatgagtcac ctactgggga accggtaccg gctgggggag cggatcgccg acggcggcat
     2341 ggggtcggtg taccgcgcgg tcgacgacaa cctcggccgg ccggtcgcgg tcaaggtgct
     2401 gcggcgggag ctggtcaacg a
//
LOCUS       DASSWR010000557         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_78053,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000557 DASSWR010000000
VERSION     DASSWR010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78053"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1192)
                     /locus_tag="VFO47_09340"
     CDS             complement(386..1192)
                     /locus_tag="VFO47_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET8788939.1"
                     /translation="MSADVFAAMEEARRAGRTVIMGTTVKTEGDPPSAPGNRALFTPD
                     GALVAGTIGCNGLDRQAGRDGAGLLAAGERSGIRAYTSFEGEPGEGVVEVFLEAFAGP
                     PRLVVGGGGPVAEALATIGGVVGLAVRAEPPGSAGFKEATQGLGPDDAVVFVDHDDPA
                     LVPALLGLLGGGAGYVGVMGSRRHTPGFVERLRQARVDLSALHSPTGLDLGARRPPEI
                     ALSILAEMMAVTHGRPGGSLSIVQRPSRQPEPEARRGAAPGRSGAEESPE"
     gene            complement(1189..>3104)
                     /locus_tag="VFO47_09345"
     CDS             complement(1189..>3104)
                     /locus_tag="VFO47_09345"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET8788940.1"
                     /translation="LEQHLVSGRLEAGEEVALRIDQTLLQDATGTMACMEFEALGLDR
                     VRVDLAVQYVDHNMLGFDHRNADDHLFLQGCAARYGLVYSRAGNGICHQVHTERFARP
                     GATLLGADSHTPTSGACGSIAIGAGGLEVALAMAGHPFQTPTPIVVGVRLEGELPPWV
                     ASKDLILWLIREYTVKGGLNRIFEFTGPGVAGLAVTQRATVCNMITELGGTTAIFPSD
                     EQTRRFLARQRREDQWVELGPDPDAEYDEEVHLDLATLEPLIARPHQPDNVVPVEEVA
                     GTPVFQVCFGSSVNSWYEDLAIPAAMLRGQHLPPTTVGTVSPGSRQILTTITTSGVLE
                     DLERAGLRILEPACGPCVGIGQAPPTGKASVRTFNRNFKGRSGTVDDQVYLASPATTA
                     ATGLHGRITDPRSLGDPPEVDDPDPVVDDFMLLAPPPPEEAERIEIVRGPNIKEPPIP
                     PPLPEAFGGRVLIVLEDNISTGSMAPDGAIVMADRSNVPAIAEYTFMKEDPEFVQRAK
                     DWEGGFIVAGDNYGQGSSREHAAMAPLALGVRCVMAQGFARIHRRNLIAQGLLPLVID
                     EETHDRLEVGDELAVADLRQAVEGAAEQVEVRVEGKDGFTAALDLSPREREVVLAGGV
                     LNQLREQEEREEA"
BASE COUNT          395 a         1113 c         1167 g          429 t
ORIGIN      
        1 gtcagagcgg acccagatcc gctcctccgc cctggtcacc gggacgggaa cgcggccagg
       61 tcggccacgg cctcgtcgaa ggtctcgtag cagcgcagcg gggtgctgcg gatgatctgc
      121 gcacggcaca ccgggaaagc agggcaaact ggggattgac cgaatccggg tcgtctggca
      181 gagtccggag cgacggcacg gtggcctttc gtatgtcacc gtgctgttga acggtaggtc
      241 tggcggtcac gccgggccgt gcgccctggt ctcttcccgt gactgggacg tgagccgacc
      301 cgccccccga ggcgggctcg gcgatggagg tgccgttcgg gggatggaag gccccgagga
      361 gaaccgcggg ctccgtgccc gaggcctact cggggctttc ctctgcccct gaccgaccag
      421 gggctgcacc gcggcgcgcc tctggctctg gctgccggct gggccgttgc acgatgctga
      481 ggctgccccc gggacggccg tgggtgacgg ccatcatctc ggccaggatc gacagggcga
      541 tctccggtgg ccggcgggcc cccaggtcca ggccggtcgg gctgtgcagg gcgctcaggt
      601 cgaccctggc ctggcgcagc cgctccacga accccggggt gtgccggcgg ctgcccatca
      661 cgccgacgta gccggccccg ccgccgagca ggccgagcag ggccgggacc agggccgggt
      721 cgtcgtggtc gacgaacacc acggcgtcgt ccgggcccag gccctgggtg gcctccttga
      781 agccggccga gccgggcggc tcggcccgca cggccaggcc gacgaccccg ccgatggtgg
      841 ccagggcctc ggcgaccggg ccgccgcccc cgacgaccag ccgcggcggg ccggcgaagg
      901 cctccaggaa cacctcgacc acgccctcgc cgggctcgcc ctcgaagctg gtgtaggcgc
      961 ggatgcccga ccgctccccc gccgccagca gcccggcccc gtcccggccg gcctggcggt
     1021 cgaggccgtt gcagccgatc gtgcccgcga ccagcgcccc gtcgggcgtg aacagggccc
     1081 ggttcccggg cgcggacggc gggtcgccct cggtcttcac ggtggtgccc atgatcaccg
     1141 tgcgcccggc ccgccgggcc tcctccatgg ccgcgaacac gtccgcgctc atgcttcctc
     1201 cctctcctcc tgctcgcgga gctggttgag gacgccgccg gccaggacga cctcgcgctc
     1261 gcggggcgac aggtcgaggg cggcggtgaa gccgtccttg ccctcgaccc gcacctcgac
     1321 ctgctccgcc gcgccctcca cggcctggcg gaggtcggcg acggccagct cgtcgccgac
     1381 ctcgagccgg tcgtgggtct cctcgtcgat gaccagcggg agcaggccct gggcgatcag
     1441 gttgcgccgg tggatgcggg cgaagccctg ggccatcacg cagcgcaccc cgagggcgag
     1501 gggggccatg gcggcgtgct cgcggctgga cccctggccg tagttgtcgc cggcgacgat
     1561 gaagccgccc tcccagtcct tggcccgctg gacgaactcc gggtcctcct tcatgaaggt
     1621 gtactcggcg atggcgggca cgttggagcg gtcggccatg acgatcgccc cgtcgggggc
     1681 catcgagccg gtggagatgt tgtcctccag gacgatcagg acccggccac cgaacgcctc
     1741 gggcagcggc ggcgggatcg gcggctcctt gatgttgggg ccgcggacga tctcgatccg
     1801 ctccgcctcc tcgggcggcg gcggggccag cagcatgaag tcgtcgacca ccgggtcggg
     1861 gtcgtcgacc tccggcgggt cgcccaggct acgggggtcg gtgatccggc cgtgcagccc
     1921 ggtggccgcg gtggtggccg gcgaggccag gtagacctgg tcgtcgacgg tgcccgagcg
     1981 gcctttgaaa ttgcggttga aggtccggac gctggccttg ccggtcggcg gagcctggcc
     2041 gatgccgacg cagggcccgc aggccggctc caggatgcgc agcccggccc gctccaggtc
     2101 ctccagcacc cccgaggtgg tgatggtggt gaggatctgg cggctgcccg gcgacacggt
     2161 cccgacggtg gtcggcggca ggtgctggcc gcgcagcatc gccgccggga tggccaggtc
     2221 ctcgtaccag gagttcacgc tcgacccgaa gcagacctgg aacaccgggg tgccggcgac
     2281 ctcctccacc gggaccacgt tgtcgggctg gtggggcctg gcgatcagcg gctccagggt
     2341 ggccaggtcg aggtggacct cctcgtcgta ctcggcatcc ggatccgggc cgagctcgac
     2401 ccactggtcc tcgcgccgct ggcgggccag gaaccggcgg gtctgctcat ccgacgggaa
     2461 gatggccgtg gtgccgccca gctcggtgat catgttgcac accgtggccc gctgggtgac
     2521 ggccaggccg gccacgcccg ggccggtgaa ctcgaagatg cggttgaggc cgcccttgac
     2581 cgtgtactcg cggatcagcc acaggatcag gtccttggag gcgacccagg gcggcagctc
     2641 gccctccagg cgcacgccga ccacgatcgg ggtcggggtc tggaacgggt ggccggccat
     2701 ggccagggcg acctcgagcc cgccggcgcc gatggcgatc gacccacacg cccccgaggt
     2761 cggggtatgg gagtcggccc cgagcagggt cgccccggga cgggcgaacc gctcggtgtg
     2821 gacctggtgg cagatgccgt tgccggcccg cgagtagacg aggccgtagc gggccgcgca
     2881 gccctgcagg aacaggtggt cgtcggcgtt gcggtggtcg aagccgagca tgttgtggtc
     2941 gacgtactgc acggccaggt ccacccggac ccggtccagc cccagggcct cgaactccat
     3001 gcaggccatc gtcccggtgg cgtcctggag cagcgtctgg tcgatgcgca gggcgacctc
     3061 ctcgcccgcc tccagccgcc ccgagaccag gtgttgctcg agga
//
LOCUS       DASSWR010000558         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_218708,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000558 DASSWR010000000
VERSION     DASSWR010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218708"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2670)
                     /gene="cphA"
                     /locus_tag="VFO47_09350"
     CDS             complement(<1..2670)
                     /gene="cphA"
                     /locus_tag="VFO47_09350"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET8788941.1"
                     /translation="MRILEARVYRGPTFWSYDKAIHLLVDLGSLEDFPSNTIPGFTDD
                     LLELLPGLVDHTCGVGRRGGFVERLREGTWLGHVAEHVALQLQREAGGEQRRGKTRSA
                     RTRGQYNVIYGYGDDQVGLAAGRLAVRLVNHLVEREPGFDFEAELERFILRAERTAFG
                     PSTQAILDEAAARDIPWLRLNEYSLVQLGQGIYQQRIRATMTSKTSSLAVDVAGDKEL
                     TTRLLAAAGLPVPRSYPVRSAEDALAAARRLGFPLVVKPLDGNHGRGVHLDLRSEEEV
                     AAAFPAAQAEARRGTVLVETYVSGNDYRVLVVGGRMVAIAERVPAHVVGDGEHTVAEL
                     VGITNADPRRGVGHEKVLTRIRVDQAAVELVRDQGFELDDVPPEGAMVKLTLTGNMST
                     GGISIDRTDEAHPDNVEIAEEAARVVGLDVAGIDFIAPDLAEPVRETGGAIVEVNAAP
                     GFRMHTHPTVGEPQYVAKPVVDLLFPPGTPSRIPIVAVTGTNGKTTTARMIAHIFKGM
                     GRQVGLTSTDGILIDERLVVTADASGPRSARMVLQNPRVDFAVFEVARGGILREGLGY
                     ERNDVGVVLNIGADHLGLRGIDTLKQLAAVKQVVIEAVPRDGHGVLNADDPLVAGMRR
                     HCSGELVYFSMQDGNELVESHCRRGGRAIVLEDGRLGEGIVLRQGRRSTLIAPTGAVP
                     ATFGGRARMNVANAMAAAAAAMAAGAHVHDIRAGLRSFTTSYEVAPGRLNLFEVDGYR
                     VIVDYCHNAAGMRMLGDFVDRLATPPKGGDIMARRRTGVIASPGDRRDKDIRELGEVA
                     ARHFDALIVREDRNLRGREPGEVAELVAAGARAGAADGGRCRSLELVLDELEASRLAL
                     DRANPGDLVVLCADRVESIVDELQSRTRASGAAG"
BASE COUNT          342 a         1026 c         1026 g          377 t
ORIGIN      
        1 gccagcggct ccggacgccc tagtccggga ctggagctcg tccacgatgc tctcgacccg
       61 gtcggcgcag agcaccacca ggtcgcccgg gttggcccgg tcgagggcga ggcgggaggc
      121 ctccagctcg tccagcacca gctccaggct gcggcagcgg cccccgtcgg cggcccccgc
      181 ccgggcgccg gcggccacca gctcggccac ctcgcccggc tcgcggccac gcaggttgcg
      241 gtcctcgcgc acgatcaggg cgtcgaagtg gcgggccgcc acctcgccca gctcgcggat
      301 gtccttgtcg cggcggtcgc cggggctggc gatcaccccg gtccgccgcc tggccatgat
      361 gtcgcccccc ttgggcgggg tggccaggcg gtcgacgaag tcgccgagca tgcgcatgcc
      421 ggccgcgttg tggcagtagt cgacgatcac ccggtagccg tccacctcga acaggttgag
      481 ccgccccggg gccacctcgt aggaggtggt gaacgagcgc aggccggccc ggatgtcgtg
      541 gacgtgggcg cctgcggcca tggcggccgc ggccgcggcc atggcgttgg ccacgttcat
      601 gcgggcccgg cccccgaagg tcgccgggac cgccccggtg ggcgcgatca gggtcgagcg
      661 ccggccctgg cgcagcacga tcccctcgcc caggcggccg tcctcgagca cgatcgcccg
      721 cccgccccgg cggcagtggc tctcgaccag ctcgttgccg tcctgcatgg agaagtagac
      781 cagctcgccc gagcagtgcc gtcgcatccc ggcgaccagc gggtcgtcgg cgttgaggac
      841 cccgtggccg tcgcggggga cggcctcgat caccacctgc ttgaccgcgg ccagctgctt
      901 gagggtgtcg atgccgcgca gcccgagatg gtcggcgccg atgttgagga ccaccccgac
      961 gtcgttgcgc tcgtacccga ggccctcgcg caggatcccg cccctggcca cctcgaacac
     1021 agcgaagtcg acccgcgggt tctggagcac catccgggcc gagcgcggcc ccgaggcgtc
     1081 ggccgtgacc accagccgct cgtcgatcag gatgccgtcg gtcgaggtca ggccgacctg
     1141 gcggcccatc cccttgaaga tgtgggcgat catccgggcc gtggtcgtct tgccgttggt
     1201 gccggtcacg gccacgatcg ggatgcgcga cggggtgccg ggcgggaaca ggagatctac
     1261 caccggcttg gccacgtact ggggctcgcc gaccgtcggg tgggtgtgca tgcggaaccc
     1321 gggggccgcg ttgacctcga cgatcgcccc gccggtctcg cgcaccggct cggccaggtc
     1381 gggggcgatg aagtcgatcc cggccacgtc caggccgacc acccgggccg cctcctcggc
     1441 gatctccacg ttgtcggggt gggcctcgtc ggtccggtcg atcgagatgc cgccggtcga
     1501 catgttgccg gtcagggtca gcttgaccat ggcgccctcg ggcggcacgt cgtccagctc
     1561 gaagccctgg tcgcggacca gctcgaccgc ggcctggtcg acccggatgc gggtcaggac
     1621 cttctcgtgg ccgacgccgc ggcgggggtc ggcgttggtg atcccgacca gctcggccac
     1681 ggtgtgctcg ccgtcgccga ccacatgggc cgggacccgc tcggcgatgg ccaccatcct
     1741 gccgcccacc accagcaccc ggtagtcgtt gccggaaaca taggtctcga ccaggaccgt
     1801 gccccgccgg gcctcggcct gggcggccgg gaaggcggcg gccacctcct cctcgctgcg
     1861 caggtcgagg tgcacgccgc ggccgtggtt gccgtccagc ggcttcacca ccagcgggaa
     1921 cccgaggcgc cgggccgcgg ccagggcgtc ctcggccgac cggaccgggt aggagcgggg
     1981 cacgggcagc ccggccgcgg ccagcagccg ggtcgtcagc tccttgtcgc cggccacgtc
     2041 gaccgccagc gagctcgtct tggaggtcat ggtggcccgg atgcgctgct ggtagatgcc
     2101 ctggccgagc tggacgagcg agtactcgtt cagccgcagc caggggatgt cgcgggccgc
     2161 cgcctcgtcg aggatggcct gggtcgaggg gccgaaggcg gtccgctcgg cccgcaggat
     2221 gaaccgctcc agctcggcct cgaagtcgaa gcccggctcc cgctcgacca ggtggttgac
     2281 cagccgcacg gccaggcggc cggcggccag cccgacctgg tcgtcgccgt agccgtagat
     2341 cacgttgtac tggccgcgcg tgcgggcgct gcgggtcttg ccccggcgct gctccccgcc
     2401 ggcctcccgc tggagctgga gggcgacgtg ctcggccacg tgccccagcc aggtgccctc
     2461 acggagccgc tccacgaacc cgccccgccg gccgaccccg caggtgtggt cgaccagacc
     2521 gggcagcagc tccagcaggt cgtcggtgaa gcccgggatc gtgttcgagg ggaagtcctc
     2581 cagcgagccg aggtcgacca gcaggtggat ggccttgtcg tacgaccaga aggtgggccc
     2641 ccggtagacg cgggcctcca ggatccgcag atcgggccga gggccgccgg gcccggggct
     2701 gtcgggctgg gggctgtcgg gcccggggct gtggacagcc ggtcgcgcgc cttcccgccg
     2761 gcggctaccc t
//
LOCUS       DASSWR010000559         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_332330,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000559 DASSWR010000000
VERSION     DASSWR010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332330"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1383
                     /gene="murA"
                     /locus_tag="VFO47_09355"
     CDS             106..1383
                     /gene="murA"
                     /locus_tag="VFO47_09355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET8788942.1"
                     /translation="MESFVIKGGQPLSGVIRPAGNKNAALPIVAASLLADEPVVLRNV
                     PHILDVETMLELVASTGATVERTGPNEVRIDPRGLRRASLDPVLCTRIRASVLLAAPL
                     VARTGHCLLPPPGGDVIGRRRLDTHLLAFRSLGVHASFDRALELEVGRLRGASMFLDE
                     ASVTATENAIMAAVLAEGTTVIGNAACEPHVQDLCRFLVGLGARISGIGSNLLTIEGV
                     ERLRGGEHTIGTDYIEVASFAALAAVTGGELTIEGVVEDDLRPVEVGFYRLGLTWELD
                     GDRLRVPRDQALEVEDDLGQAIPKLDDGPWPAFPADLTSIAVAVATQARGTVLIFEKM
                     FENRLVFVDKLVGMGARIIVCDPHRVVVNGPTRLYGERLESPDIRAGMALLLAALAAD
                     GESVIGNVGQIDRGYERVDERLRTLGANIERTG"
     gene            1402..1953
                     /locus_tag="VFO47_09360"
     CDS             1402..1953
                     /locus_tag="VFO47_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788943.1"
                     /translation="MALGAGDCERLHDGLIAQPVNTASALAFVAVGAWLAGRGLGAGA
                     GPARPASVGFGLVVAAAGVGSVAYHGPGSPAARLLHDGTLYVVVGFVVWQEVARRVGR
                     SRRARAGMPWVGWTPRRRTAYRAALAAAAAGAACWWLGRTASPVCDPDSLLQGHAAWH
                     LLAAAALACWAVAVLDPPAQARS"
     gene            complement(1938..>2457)
                     /locus_tag="VFO47_09365"
     CDS             complement(1938..>2457)
                     /locus_tag="VFO47_09365"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET8788944.1"
                     /translation="LDGFLEAALGYLVREKDLRSWVPGPGWANAVGHTADLLMMLARS
                     PHLGAAELQRILDAIADRLLTPVPEVFVHHEDERLAYAVLNALRRNLIDRSWLVAWLD
                     RFTAAPGQESWRAAYASEAESAARANVTAFLRSLYFQLILTESPPPDAQRSSDAVLVT
                     IRRSDIGFYDLA"
BASE COUNT          298 a          895 c          936 g          328 t
ORIGIN      
        1 acggacccgg tacgacaggc gtcgtggccg gagcttgacc aggtcgagat gcggcagggg
       61 tagagtaggc ggccgtgccc cgaacggccg cgagctggga gcgtcatgga gagcttcgtc
      121 atcaagggcg ggcagccgct gagcggcgtc atccgcccgg ccgggaacaa gaacgccgcg
      181 ctgcccattg tggccgcctc cctgctggcc gacgagccgg tggtgctgcg caacgtcccc
      241 cacatcctgg acgttgagac gatgctggag ctggtcgcct cgaccggcgc cacggtggag
      301 cggaccggcc ccaacgaggt ccgcatcgac ccgcgcggcc tgcgccgggc atcactcgac
      361 ccggtgctgt gcacccgcat ccgggcgtcg gtgctgctgg cggcgccgct ggtggccagg
      421 accggccact gcctgctgcc gccccccggc ggcgacgtga tcggccggcg gcggctcgac
      481 acccatctgc tggccttccg cagcctcggc gtccatgcca gcttcgaccg ggccctggag
      541 ctggaggtgg ggcggctccg cggggcgtcg atgttcctcg acgaggcgtc ggtgaccgcc
      601 accgagaacg ccatcatggc cgccgtcctg gccgagggca cgaccgtcat cggcaacgcc
      661 gcctgcgagc cccacgtcca ggacctgtgc cgcttcctgg tcggcctggg cgcccgcatc
      721 tccggcatcg ggtcgaacct cctgaccatc gagggggtgg agcggctccg ggggggcgag
      781 cacaccatcg ggaccgacta catcgaggtg gccagcttcg ccgccctggc cgccgtgacc
      841 gggggtgagc tgacgatcga gggcgtggtc gaggacgacc tgcgcccggt cgaggtcggc
      901 ttctaccgcc tggggctgac ctgggagctg gacggggacc gcctgcgggt gccgcgcgac
      961 caggccctgg aggtcgagga cgacctcggc caggccatcc ccaagctcga cgacgggccc
     1021 tggccggcgt tcccggccga cctgacctcg atcgcggtcg cggtcgccac ccaggccagg
     1081 ggcaccgtgc tgatcttcga gaagatgttc gagaaccggc tggtgttcgt ggacaagctg
     1141 gtcggcatgg gggcccggat catcgtctgc gacccccacc gggtggtcgt caacggcccc
     1201 acccgcctgt acggggagcg gctggagtcg cccgacatcc gcgccggcat ggccctgctg
     1261 ctggccgccc tggccgccga cggcgagtcg gtcatcggca acgtcggcca gatcgaccgg
     1321 ggctacgagc gcgtcgacga gcggctccgc accctcgggg ccaacatcga gcgcaccggc
     1381 tgacgtgctg ggagggcggg attggcccta ggcgccggcg actgcgagcg gctccacgac
     1441 ggcctgatcg cccagccggt caacaccgcc tcggccctgg ccttcgtcgc cgtcggcgcc
     1501 tggctggccg gccggggcct aggtgccggc gccggtcccg cccggccggc atcggtcgga
     1561 ttcgggctgg tcgtggccgc cgccggcgtc ggcagcgtcg cctaccacgg ccccggctcc
     1621 cccgcggccc ggctgctgca cgacggcacc ctgtacgtgg tggtcgggtt cgtcgtctgg
     1681 caggaggtcg cccggcgggt cgggcggtcc cgccgagccc gggccgggat gccctgggtc
     1741 ggctggaccc cgcgccgccg caccgcctac cgggccgccc tggccgcggc ggccgccggc
     1801 gccgcctgct ggtggctggg ccgcaccgcc agccccgtgt gcgaccccga cagcctcctc
     1861 cagggccacg ccgcctggca cctgctggcc gcggccgccc tggcctgctg ggcggtggcc
     1921 gtcctcgacc cgccggctca ggccaggtcg tagaagccga tgtcggaacg cctgatcgtg
     1981 accaggaccg cgtccgagct gcgctgggcg tcggggggcg ggctctcggt caggatcagc
     2041 tggaagtaga ggctgcgcag gaaggcggtg acgttggccc gggccgccga ctcggcctcg
     2101 ctggcgtagg cggcccgcca ggactcctgg ccgggggcgg cggtgaagcg gtccagccag
     2161 gcgaccagcc agctgcggtc gatcaggttg cgccggagcg cgttgaggac ggcgtaggcc
     2221 aggcgctcgt cctcgtggtg gacgaagacc tccgggacgg gggtgagcag ccggtcggcg
     2281 atcgcgtcca ggatccgctg gagctcggcc gcgcccaggt gggggctgcg ggccagcatc
     2341 atcagcaggt cggcggtgtg gccgacggcg ttggcccagc ccggccccgg gacccagctg
     2401 cgcaggtcct tctcgcgtac caggtagccg agggccgcct ccaggaagcc gtccagg
//
LOCUS       DASSWR010000560         4705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_981353,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000560 DASSWR010000000
VERSION     DASSWR010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981353"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFO47_09370"
     CDS             <1..1033
                     /locus_tag="VFO47_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET8788945.1"
                     /translation="VLYLSMTVMFVYVYNLPRSAVPARRIILAMTLFWATVICFGFAA
                     LASPSFRLTTPASLLLPETLAANPFAQDLFSARLAQVQTFIGYALPRPAAPFTYTNEW
                     GGAVGLLTPMALAGLGMLRSYLTRNVVRLLLVASLVPIVISANRGLWVGLGVGLVYAA
                     VRVALRGNARALVAILAFLAVVAALVAFTPLERYVSDRLAHPHSNERRESLSSQALRG
                     AQERPLLGYGGPRESPDLVNAPEIGTHGQLYLVVFSQGIVGLAFFLGWWLWVLWSSHR
                     AATGPPLWCHVMLLIGVLEFAYYDMMPTQLHLMMVVAAVAWRERGAAQAAAAERRSLT
                     QTRESLQTT"
     gene            1030..1899
                     /locus_tag="VFO47_09375"
     CDS             1030..1899
                     /locus_tag="VFO47_09375"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET8788946.1"
                     /translation="MTQANGATKLARRAARWSAQAYARPTAGLRLLPDYLIIGAQRAG
                     TTSLHRYLIQHPGVRTTLRTKGVHFFDVAYGRGMGWYASRFPTRLTAWWVARRHRVEL
                     RTGEASPYYLFHPLVPERVAGHLPQVKLIALLRDPVGRAYSHYQHEVARGFETLSFEE
                     AIEAEPARLAGEAERMLAEPLYNSFAHQHHSYLARGRYLEQLERWRARFDDRQLLVLS
                     SERFFAEPERTFRQVLDFLELPAFTPDAFEKHNGYDYRKLGTDVHRRLADHFREPNQR
                     LYASLGDDFGWSA"
     gene            1906..3498
                     /locus_tag="VFO47_09380"
     CDS             1906..3498
                     /locus_tag="VFO47_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET8788947.1"
                     /translation="METAAAAPAAGSETRQDLTTLARGGALNLAGAVATGLLHFVLLV
                     VVTRGLGAQGTGAFYEAVALFLILSSAAALGSDVGLSRMVPRYRALGRVRDLRRGLAV
                     GLWPVAAAGCLLGLLTWWYAAELADVFSRHGEAETAQLAGFIRTFALFVPVAALSLAV
                     FAATRGFATMRPTVYLDKIVRPALQPLLMLPVILAGAGSTVVALAYLGPYLPALAAGL
                     VWMGLLLRRAERRREAEGAPSRQRPRSMGQLWGEYWRFTGPRGLAAVFQTTSLWLNTL
                     LIGALRSIKEAGVYAASSRYLAMAAMAAVAIRQVLAPKLSELLARRSTERAAAAYQTT
                     TSWMIALNWPVYLALLTFGPALLQVFGRDFAGGEVVLVVLAATMLAATAVGPVDVVLL
                     MGGRSSWNLVNTVISLGANLALNFALTPRYGLAGAAVAFAAGILLNNLLPLVQVWRSM
                     GLHPFGPGTGVAAALSAVSFGLVGLVLRALAGPTVAGFVAYAVAGCGLYAVLLWRFRD
                     RLEWEALSGILRRRRSRSLVEP"
     gene            3498..>4705
                     /locus_tag="VFO47_09385"
     CDS             3498..>4705
                     /locus_tag="VFO47_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET8788948.1"
                     /translation="MGGRTLVAGLVVAVLVLGGVAFAAVRGSGGPGGAAGPGGAGRRP
                     NVLVLVTDDARAETLQAMPKTRRWLADGGVTYSQGFATTPSCCPSRASILSGRYVHNH
                     GVLRQRLGERLDQRTTLARHLKDAGYGTAMAGKFLNRWSLRRPPPHFDRYAQANGGYY
                     DQLWQVDGTLRRVPTYSTTFIGDQALAYLEEFEADDDRPWFLYLAPFAPHDPRVPEPS
                     YAEATFPELDGVGGTGAEVAGAPAYLRQRPPADSREVADLRTGQLRTLLSVDDLVDRV
                     LGRLAGTGELDDTLVFYLSDNGYSWGEHRHVGKFVPYTESIKVPFLVRWPGRLPAGTV
                     DDRLAATVDIKPTVLAAAGIRPADGDTVDGRSLLDGHRRERLLAEYFQDQANAPGIRD
                     WAALRTAGWQ"
BASE COUNT          534 a         1831 c         1651 g          689 t
ORIGIN      
        1 cgtgctgtac ctgtccatga cggtgatgtt cgtctacgtc tacaacctgc cccgctcggc
       61 ggtcccggcc cggcggatca tcctggccat gacgctgttc tgggcgacgg tgatctgctt
      121 cggcttcgcc gccctggcct cgcccagctt ccggctcacc accccggcgt cgctgctgct
      181 gcccgagacg ctggcggcca acccgttcgc ccaggacctg ttcagcgccc gcctggccca
      241 ggtccagacc ttcatcggct acgccctgcc ccggccggcg gccccgttca cctacaccaa
      301 cgagtggggc ggggcggtgg ggctgctcac ccccatggcc ctggccgggc tgggcatgct
      361 ccgctcctac ctgacccgca acgtggtccg gctgctgctg gtcgcctccc tggtcccgat
      421 cgtgatctcg gccaaccgcg gcctgtgggt cgggctcgga gtgggcctgg tgtacgcggc
      481 cgtccgggtg gccctgcgcg gcaacgcccg ggccctggtg gccatcctgg ccttcctggc
      541 cgtggtcgcc gccctggtcg ccttcacccc gctggagcgc tacgtcagcg accgcctggc
      601 ccacccgcac agcaacgagc gccgcgagag cctcagctcc caggccctac ggggcgcgca
      661 ggagcggccg ctgctcggct acggcgggcc gcgggagtcg cccgacctgg tcaacgcccc
      721 cgagatcggc acccacggcc agctctacct ggtcgtgttc tcccagggca tcgtcggcct
      781 ggccttcttc ctgggctggt ggctgtgggt gctgtggagc tcccaccggg cggccaccgg
      841 gccgcccctg tggtgccacg tgatgctgct gatcggggtc ctcgagttcg cctactacga
      901 catgatgccc acccagctgc acctgatgat ggtggtggcg gccgtggcct ggcgggagcg
      961 gggggccgct caagccgccg ccgccgaacg ccgatccttg acccagaccc gggagagcct
     1021 gcagacgaca tgacccaagc caacggcgcc accaagctgg ccaggagagc ggcgcgctgg
     1081 agcgcccagg cgtacgcacg cccaacggcc gggctgcggc tgctgcccga ctacctcatc
     1141 atcggcgccc agcgggccgg cacgacctcg ctgcaccgct acctgatcca gcatcccggg
     1201 gtccggacca cactgcgcac caaaggggtg cacttcttcg acgtcgccta cggccgcggg
     1261 atgggctggt acgcctcgcg cttcccgacc cggctgaccg cctggtgggt ggccaggcgc
     1321 caccgggtcg agctgcgcac cggcgaggcc agcccctact acctgttcca cccgctggtg
     1381 cccgagcggg tggccgggca cctgccccag gtcaagctga tcgccctgct acgcgacccg
     1441 gtcgggcggg cctactcgca ctaccagcac gaggtcgccc gcggcttcga gacgctgtcg
     1501 ttcgaggagg cgatcgaggc cgagccggcc cggctggccg gcgaggccga gcgcatgctg
     1561 gccgagccgc tctacaacag cttcgcccac cagcaccact cctacctggc caggggccgc
     1621 tacctcgagc agctggagcg gtggcgggcc cggttcgacg accggcagct gctggtgctg
     1681 tccagcgagc gcttcttcgc cgagccggag cgcaccttcc gccaggtcct cgacttcctc
     1741 gagctgccgg cgttcacccc cgacgccttc gagaagcaca acggctacga ctaccggaag
     1801 ctgggcaccg acgtccaccg gcgcctggcc gaccacttcc gggagccgaa ccagcggctg
     1861 tacgcgtcgc tcggcgacga cttcggctgg agcgcctagc ctccgatgga gaccgccgct
     1921 gccgcgccgg ccgccggctc cgagacccgg caggacctga ccaccctggc cagggggggc
     1981 gccctcaacc tggccggcgc cgtggccacc gggctgctcc acttcgtgct gctggtggtc
     2041 gtcaccaggg gcctgggggc ccagggcacc ggcgccttct acgaggcggt ggccctgttc
     2101 ctgatcctgt ccagcgcggc cgcgctcggc tccgacgtcg ggctgtcacg gatggtgccg
     2161 cgctaccggg ccctggggcg ggtgcgcgac ctgcgccgcg gcctggccgt cggcctctgg
     2221 ccggtggccg ccgccggctg cctgctcggg ctgctgacct ggtggtacgc ggccgagctg
     2281 gccgacgtct tctcccgcca cggcgaggcc gagacggccc agctggccgg cttcatccgc
     2341 accttcgccc tgttcgtgcc cgtggcggcg ctgtcgctgg ccgtgttcgc ggccacccgc
     2401 ggcttcgcca ccatgcgccc gacggtgtac ctggacaaga tcgtccgccc cgccctccag
     2461 cccctgctga tgctgccggt gatcctggcc ggggcgggca gcaccgtggt cgccctcgcc
     2521 tacctcgggc cgtacctgcc ggcgctggcc gcgggcctgg tctggatggg gctgctgctg
     2581 cgccgggccg aacgccgccg cgaggccgag ggcgctccgt cgcggcagcg gccccggtcg
     2641 atgggccagc tgtgggggga gtactggcgc ttcaccggcc cccgcgggct ggccgcggtc
     2701 ttccagacca cctcgctgtg gctcaacacc ctgctgatcg gggcgctgcg ttccatcaag
     2761 gaagccgggg tgtacgcggc cagctcgcgc tacctggcca tggcggccat ggccgcggtg
     2821 gccatccgcc aggtcctcgc ccccaagctg agcgagctgc tggcccgccg cagcaccgag
     2881 cgggccgcgg ccgcctacca gacgaccacc agctggatga tcgccctcaa ctggcccgtc
     2941 tacctggccc tgctcacctt cggcccggcc ctgctccagg tgttcggccg cgacttcgcc
     3001 ggcggcgagg tggtcctggt cgtgctggcg gcgaccatgc tggccgccac cgccgtcggc
     3061 ccggtcgacg tggtcctgct gatgggtggc cgcagctcct ggaacctggt caacacggtg
     3121 atctcgctgg gggccaacct ggccctcaac ttcgccctca ccccccgcta cggcctggcc
     3181 ggggcggcgg tcgcgttcgc cgccggcatc ctgctcaaca acctgctccc gctggtccag
     3241 gtgtggcggt cgatgggcct gcacccgttc gggccgggca cgggggtggc cgcggccctg
     3301 tcggcggtca gcttcgggct ggtcggcctg gtgctgcggg ccctggccgg gccgaccgtg
     3361 gccgggttcg tggcctacgc cgtggccggc tgcgggctgt acgcggtcct gctgtggcgc
     3421 ttccgcgacc gcctggagtg ggaggcgctc agcggcatcc tgcgccgccg ccgctcccgg
     3481 tccctagtgg aaccgtgatg ggcgggcgca cgctggtggc ggggctggtc gtggccgtgc
     3541 tcgtccttgg cggggtggcg ttcgccgccg tccgcgggtc gggcgggccg ggcggcgccg
     3601 ccggcccggg tggggcgggg cggcggccga acgtgctcgt gctggtgacc gacgacgccc
     3661 gggccgagac cctccaggcg atgccgaaga cgcggcgctg gctggccgac ggcggggtca
     3721 cctactccca ggggttcgcg accacgccca gctgctgccc gtcgcgggcg tcgatcctga
     3781 gcggccgcta cgtccacaac cacggggtgc tccgccagcg gctgggggag cggctcgacc
     3841 agcggaccac cctggcccgc cacctcaagg acgccggcta cggcacggcc atggccggca
     3901 agttcctcaa ccgctggtcg ctgcggcggc cgccgcccca cttcgaccgc tacgcccagg
     3961 ccaacggcgg ctactacgac cagctgtggc aggtcgacgg caccctccgc cgggtcccca
     4021 cctactcgac gaccttcatc ggcgaccagg ccctcgccta cctggaggag ttcgaggccg
     4081 acgacgaccg cccctggttc ctctacctgg cgccgttcgc gccccacgac ccgcgcgtgc
     4141 ccgagcccag ctacgccgag gccaccttcc cggagctgga cggggtcggc ggcaccgggg
     4201 cggaggtggc cggcgccccc gcgtacctgc ggcagcggcc gccggccgac agccgggagg
     4261 tggccgacct gcgcaccggc cagctccgca ccctgctgtc ggtcgacgac ctggtcgacc
     4321 gggtcctggg ccggctcgcc ggcaccggcg agctggacga cacgctggtc ttctacctgt
     4381 ccgacaacgg ctactcctgg ggggagcacc gccacgtggg caagttcgtg ccctacaccg
     4441 agtccatcaa ggtcccgttc ctggtccgct ggccggggcg gctgcccgcc ggcacggtcg
     4501 acgaccgcct ggccgccacc gtcgacatca agccgacggt cctggccgcg gccggcatcc
     4561 ggcccgccga cggcgacacc gtcgacggcc gctccctgct cgacggccac cgccgggagc
     4621 ggctgctggc cgagtacttc caggaccagg ccaacgcccc cggcatccgg gactgggcgg
     4681 ccctgcgcac cgccggctgg cagta
//
LOCUS       DASSWR010000561         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_134637,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000561 DASSWR010000000
VERSION     DASSWR010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134637"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..540
                     /locus_tag="VFO47_09390"
     CDS             187..540
                     /locus_tag="VFO47_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8788949.1"
                     /translation="MIDCSDFNAMSGFWQEALGYVPREPAEDGWVVLRDPGGTEVNVS
                     LQQVPEPRSGKNRLHFDLYTDDQEGEGEVERLLAIGATRHPRTSGPDEDFVVLEDPEG
                     NLFCVVDTRPPGLRP"
     gene            complement(681..1898)
                     /locus_tag="VFO47_09395"
     CDS             complement(681..1898)
                     /locus_tag="VFO47_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788950.1"
                     /translation="MRTAARVLPVVLAILLLVGLLSPPAGAVPSPTPATTLQFYGGEV
                     RAVAQIGNTIYVGGSFTSVGYGSGTIARAHLVALDATTGLHVPGFDPRADDDVNALLR
                     SPDGTRLYAGGIFNNVRGCTRCDRLAMLDPATGVANTAFGPQPNAAVLKLALAGNTLV
                     AGGAFAAVGGRRRVRLAAVNATTGAVGAMALHANGAVRDLAVNPAGTTLYLAGSFTTV
                     GGATRYNFASVAIASRTLTAWNPNIHASGWGVSLSPDGATAYLTTADGNETICGAAHE
                     SVIAMPAGTAGTPPVLWHNGGAPGCPFNSGDINAVEATASAVYIGGHLTNLCTVRNTS
                     YTVRCPGALTVRSHVAALDPATGVPLAWNPGAGGTRGVLTAEALPAGLAIGGDFQTAG
                     ATHRGFALFPGTP"
     gene            complement(2030..2476)
                     /locus_tag="VFO47_09400"
     CDS             complement(2030..2476)
                     /locus_tag="VFO47_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET8788951.1"
                     /translation="MAMADRQAHTLWEGPLIGGKGDTEFASSGIGRYPVTWAARTESP
                     DGKTSPEELLAAAHATCYAMAFSHFLAQAGNPPERLHVTSTVSLDPKEGGGVVVTRSA
                     LEVNGTVPGYDQDGFQQAAEEAEKGCPISNAIRGNLEITVKATLDA"
     gene            complement(2521..3723)
                     /locus_tag="VFO47_09405"
     CDS             complement(2521..3723)
                     /locus_tag="VFO47_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8788952.1"
                     /translation="MGRRVAGEGRRVRLGTIYAAHGASHGFVLVLPAVLVTLRAEFGA
                     SFTTLGTVATVSSMLYGLGALPGGLLADRFGAPVLLRVFAGLSAVCCGLAAVAPGIWW
                     LAAALALLGAAGALYHPSGLAEVTLNAPGGGRELGVHGGFGNGGTALAPLVAGAVAAA
                     WTWRASYGLAAVAALVLLVVLVRDVPLDRRLPPQEQAASGAVGRGALAVVMFLAAAEG
                     FVFQGFVTFLPAFLAEVGGVGQAAAAKGGVLAAAVLLLGVPGQLVGGRLAETDPRRLA
                     LRYAGLYGAAVLAGLSVRAAGATPLGVALAGLFSLLIFLGQPITNQLVARSTRAGRRG
                     AAYGTYFSLSFGIGALAGVAGGVVADRSGLAAVFGFLGLVAVVNSLGGLAVRGLLGPG
                     STGRPPAS"
BASE COUNT          535 a         1363 c         1482 g          440 t
ORIGIN      
        1 taccggacct ggatcgagca ggccggcctc tcgatcgtca cccaggacgt cgttcccgag
       61 ggcgacgccg ctcacaccct gttctgggcc cgtcaggtta cggcgcggta cgacggttca
      121 tcatgacccg gtcgccgccg gcggcgtcct gagagaaagg gtggcgtagt gaggatcggc
      181 tcggtcgtga tcgactgcag cgatttcaac gcgatgtcgg ggttctggca ggaggccctc
      241 gggtacgtcc cgagggaacc cgccgaggac ggctgggtcg tcctccggga ccccggcggg
      301 accgaggtga acgtgtccct ccagcaggtc ccggagccac gttccggcaa gaaccggctg
      361 cacttcgacc tctacaccga cgaccaggag ggggaggggg aggtggagcg gctgctcgcc
      421 atcggagcga cccgccaccc ccggacctcc gggccggacg aggacttcgt cgtcctcgaa
      481 gacccggagg ggaacctgtt ctgcgtggtc gacacgcgcc cgcctggact ccggccatga
      541 cgaccggacc cgacctggcc atccggcggc tggtggccgc cgacgctccc gggtgtgacg
      601 agatcgtccg gagcctctac cacttcggcg accccggccg gcgaggacgc cgccttccag
      661 gggcgaggcc cggtaaccgc ctacggcgta cccgggaaca acgcgaaccc gcggtgggtt
      721 gcgccggcgg tttggaagtc gccgccgatg gcgaggccgg cggggagggc ttcggcggtg
      781 aggacgccgc gggtgccgcc ggcgccgggg ttccaggcga gggggacgcc ggtggccggg
      841 tcgagggcgg cgacgtggct gcggacggtg agggcgcctg ggcagcggac ggtgtaggag
      901 gtgttcctga ccgtgcagag gttggtcagg tggccgccga tgtaaacggc gctcgccgtg
      961 gcctcgacgg cgttgatgtc gccggagttg aaggggcagc caggggcgcc gccgttgtgc
     1021 cacaggaccg gcggggtgcc ggcggtgccg gccggcatgg cgatcaccga ctcgtgtgcg
     1081 gccccgcaga tcgtctcgtt gccgtcggcg gtggtcaggt aggcggtggc gccgtcgggt
     1141 gagagcgaca ccccccagcc ggaggcgtgg atgttggggt tccaggcggt cagggttcgc
     1201 gaggcgatgg ccaccgaggc gaagttgtag cgggtggcgc cgccgacggt ggtgaacgat
     1261 ccggccaggt agagcgtcgt cccggccggg ttgacggcca ggtcgcggac ggccccgttg
     1321 gcgtgcaggg ccatggcgcc gacggcgccg gtggtggcgt tgaccgcggc caggcgcacc
     1381 cggcggcggc cgccgacggc ggcgaaggcg cccccggcga ccagggtgtt gccggcaagg
     1441 gcgagcttga gcacggccgc gttgggctgc ggcccgaagg cggtgttggc cacgccggtc
     1501 gccgggtcga gcatggccag gcggtcgcag cgggtgcagc cgcggacgtt gttgaagatc
     1561 ccgccggcgt acaggcgagt gccgtcgggg ctgcgcagca gggcgttgac gtcgtcgtcg
     1621 gcccgcgggt cgaacccggg gacgtgcagc ccggtggtcg cgtccagggc gaccagatgg
     1681 gcgcgggcga tcgtgccgct gccgtagccg accgaggtga agctgccgcc gacgtagatg
     1741 gtgttgccga tctgggcgac ggcgcgcacc tcgccgccgt agaactggag ggtggtcgcc
     1801 ggggtcgggg acggcacggc gccggccggc ggggacagca gcccgacgag cagcaggatg
     1861 gcgaggacga cgggcaggac gcgggcggcg gtacgcattg atggccccac tccagacgca
     1921 ggccagacag accggcccca ggcgcagcag ggctaaacgc cgcctggacg tcggaggatg
     1981 cgcaatatga cgtagttctg gcttattctc cggtcctgcc gggccggggt caggcgtcga
     2041 gcgtggcctt gaccgtgatc tcgaggttgc cgcggatggc gttggagatc gggcagccct
     2101 tctcggcctc ctcggcggcc tgctggaagc cgtcctggtc gtacccgggg acggtgccgt
     2161 tgacctccag ggccgagcgg gtgacgacga caccgccacc ctccttgggg tcgagggaga
     2221 cggtggaggt gacgtgcagg cgctcgggcg ggttgccggc ctgggcgagg aagtgcgaga
     2281 acgccatcgc gtagcaggtg gcgtgggcgg ccgcgagcag ctcctcgggg ctggtcttgc
     2341 cgtcggggct ctcggtccgg gccgcccagg tcaccgggta gcgcccgatg ccgctggagg
     2401 cgaactcggt gtcgcccttg ccgccgatca gcggcccctc ccagagcgtg tgcgcctggc
     2461 ggtcagccat ggccatgtcg acatccctcc cgatcgatcc gtcgtccagt ccacgaaacg
     2521 ctaggacgcc gggggccggc ctgtcgaccc gggaccaagc aggccccgca cggccagccc
     2581 gccgagggag ttgaccacgg cgaccagccc caggaagccg aacacggcgg ccagccccga
     2641 ccggtcggcc accaccccgc cggccacccc ggccagggcg ccgatcccga acgacagcga
     2701 gaagtaggtg ccgtaggcgg ccccgcgccg cccggcccgg gtcgagcggg ccaccagctg
     2761 gttggtgatc ggctggccga ggaagatgag caggctgaac agcccggcca gggccacgcc
     2821 gagcggggtc gcgccggccg ccctgaccga cagcccggcc agcacggcgg ccccgtacag
     2881 gccggcgtag cggagcgcca ggcggcgcgg gtcggtctcg gccagccggc cgccgaccag
     2941 ctggccgggg acgcccagca gcagcaccgc cgccgccagc acgccgccct tggccgcggc
     3001 cgcctgcccg accccgccca cctcggccag gaacgccggc aggaaggtga cgaacccctg
     3061 gaacacgaac ccttcggccg cggccaggaa catcaccacg gccagcgccc cccggcccac
     3121 cgccccggac gccgcctgct cctgtggcgg caggcgccgg tcgagcggca cgtcccggac
     3181 gagcacgacc agcaggacca gggcggccac ggcggccagc ccgtagctcg cccgccaggt
     3241 ccaggcggcg gccaccgccc cggcgaccag cggcgccagg gcggtgccgc cgttgccgaa
     3301 gccgccgtgg acgccgagct cgcggccgcc tccgggggcg ttcagggtga cctcggccag
     3361 cccggagggg tggtagaggg ccccggccgc acccagcagg gcgagcgcag cggccagcca
     3421 ccagatcccg ggggcgaccg cggccagccc gcagcagacg gccgacaggc ccgcgaacac
     3481 ccgcagcagc accggcgccc cgaaccggtc ggccagcagc cccccgggca gggccccgag
     3541 cccgtacagc atgctgctca ccgtggccac cgtgcccagc gtggtgaacg aggccccgaa
     3601 ctcggcccgc agggtcacca gcaccgccgg cagcaccagc acgaacccgt ggctggcccc
     3661 gtgggcggcg tagatcgtcc ccagccgcac ccgccggccc tcgcccgcca ccctccgccc
     3721 catggcccga gcatgccaga cccgcccccg ggtcgtgccc gcctgggtag gttcagaaca
     3781 gggccgggcg ggccgtgacc ggccgccctg ccccgggccg
//
LOCUS       DASSWR010000562         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1092647, whole genome shotgun sequence.
ACCESSION   DASSWR010000562 DASSWR010000000
VERSION     DASSWR010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092647"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..>2371
                     /locus_tag="VFO47_09410"
     CDS             322..>2371
                     /locus_tag="VFO47_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET8788953.1"
                     /translation="MVAARRPPRRWTLAVLLLAFLALAAVGSVSRFYTDLLWFREIDK
                     AGLFWGMLRTKAFIGLLAGLGTAAIVGVNLWMVERLAPRYGLTVVARPQVERARAVLS
                     PYLRPLRIGIAAFLGLVVGLQASGLWQTFLLWSNRVAFGQRDALFGRDVGFYMFELPF
                     LRSVFGWLFTTLVLTTLLVAAGHYILGGIRPQAETNRIAAQAQSHLCVLLGLIVALKA
                     WGYWLDKYQLLFSTRGVVAGASYTDVKAQLPALEVLFWVALICAAMFFWGARRGGLLI
                     PTISIVLLAGVSLIMGGIIPAIFQRFRVEPQELARERPYIERNIGATRQSFGLSDVDT
                     DSFPAAGTLTAKVVDENRQTVENIRLWDPQVLRPAVRNLQAITQYYTFSDVDVDRYKV
                     DNELRQVMISVREVDPNGLAESARTWQNLHLAYTHGYGMVAVQVNTAAEGGQPDFIVS
                     GFNPTDPPIDVKEPRVYFGEPPPNSPEFVVANTAQGEYDAPSLTGEATSLFHYDGTGG
                     VQLSDMGRRLAFAVRFRDINLLISGNIKNESRLMFNRDIRDRVEKAAPFLQWDGDPYA
                     VVVDGRIKYVRDGYTTSSNYPYAQRIDLADAARRNELGSRGVESIGNYIRNSVNAVVD
                     AYDGTVSLYAYDEQDPILKSWGKAFPGLLKPKSSIPAELESHLRYPEDLFAVQADRYK
                     S"
BASE COUNT          343 a          889 c          767 g          372 t
ORIGIN      
        1 ggagtcgatc gagtgtcggc cgatggccga ccccacgcct tggttggacc ggtcctcccg
       61 gagccaaccc cgggaccggt cggggttggc tccgggagcc gaccggcgcc ggacggcgcc
      121 gaccggacgg agcccctcgc cgacagcggc cagcagaggt gccttcctgg tagcaggcgc
      181 gcgtggcccg gaaatgggta cgggcagcct cgccgccacc ccggcgacgg ggcacccgtc
      241 ccagtccgaa cacccgtgct agggtctgga aggaagcatc ggagtgcccg tcgccgggcg
      301 cccgtcgaaa ggtccgagct cgtggtcgca gcccgccgtc ctcctcgtcg atggacgctg
      361 gccgtcctgc tgctggcgtt cctcgccctg gccgcggtcg gcagcgtctc gcgcttctac
      421 accgacctcc tgtggttccg ggagatcgac aaggccgggc tgttctgggg catgctgcgg
      481 accaaggcct tcatcggcct gctggccggg ctggggaccg cggccatcgt cggcgtcaac
      541 ctgtggatgg tggagcggct ggccccgcgc tacggcctca ccgtggtcgc ccgcccccag
      601 gtcgagcggg cccgggccgt cctctccccg tacctgcggc cgctgcgcat cggcatcgcc
      661 gccttcctcg ggctggtcgt cggcctgcag gcgtccggcc tgtggcagac cttcctgctg
      721 tggagcaacc gggtcgcctt cggccagcgc gacgccctgt tcggccgcga cgtcggcttc
      781 tacatgttcg agctgccgtt cctgcggtcc gtcttcggct ggctgttcac caccctggtg
      841 ctcaccaccc tgctggtggc ggccgggcac tacatcctgg gcgggatccg cccccaggcc
      901 gagaccaacc gcatcgccgc ccaggcccag tcgcacctgt gcgtgctcct cggcctgatc
      961 gtcgccctca aggcctgggg ctactggctc gacaagtacc agctgctgtt ctccacccgc
     1021 ggagtggtcg ccggcgcctc ctacaccgac gtcaaggccc agctgccggc cctcgaggtg
     1081 ctgttctggg tcgctctgat ctgcgcggcg atgttcttct ggggggcgcg gcgaggcggc
     1141 ctgctcatcc ccacgatcag catcgtcctg ctcgccgggg tgtccctgat catgggcggc
     1201 atcatcccgg ccatcttcca gcgcttccgg gtcgagcccc aggagctggc ccgcgagcgg
     1261 ccctacatcg agcgcaacat cggcgccacc cgccagtcgt tcggtctcag cgacgtcgac
     1321 accgattcgt tcccggccgc cggcaccctc accgccaagg tcgtcgacga gaaccggcag
     1381 acggtcgaga acatccgcct gtgggacccg caggtcctcc ggccggcggt gcggaacctc
     1441 caggcgatca cccagtacta caccttcagc gacgtcgacg tggaccgtta caaggtcgat
     1501 aacgagctgc gccaggtgat gatctcggtc cgcgaggtcg accccaacgg cctggccgag
     1561 tcggcccgga cctggcagaa cctgcacctg gcctacaccc acggctacgg catggtggcg
     1621 gtgcaggtca acacggccgc cgagggtggc cagcccgact tcatcgtctc cgggttcaac
     1681 cccacggacc ccccgatcga tgtcaaagag ccgcgggtct acttcggcga gccgccgccc
     1741 aactcgcccg agttcgtggt cgccaacacc gcccagggcg agtatgacgc cccctcgctc
     1801 accggcgagg cgaccagtct gttccactac gacggcaccg ggggcgtgca gctgtccgac
     1861 atgggccgcc ggctcgcctt cgccgtccgc ttccgcgaca tcaacctgct gatctcgggc
     1921 aacatcaaga acgagtcccg cctgatgttc aaccgcgaca tccgcgaccg ggtcgagaag
     1981 gcggccccgt tcctgcagtg ggacggcgac ccctacgcgg tggtggtcga cggccgcatc
     2041 aagtacgtcc gcgacggcta caccacctcc agcaactacc cgtacgccca gcgcatcgac
     2101 ctggccgacg ccgcccgccg caacgagctg ggcagccgcg gggtcgaaag catcggcaac
     2161 tacatccgca actcggtcaa tgccgtggtc gacgcctacg acgggaccgt ctcgctgtac
     2221 gcctacgacg agcaggaccc gatcctcaag tcgtggggca aggcgttccc cggcctgctc
     2281 aagccgaagt cgagcatccc ggccgagctg gagtcgcacc tgcgctaccc ggaggacctg
     2341 ttcgcggtcc aggccgaccg gtacaagagc t
//
LOCUS       DASSWR010000563         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1376863, whole genome shotgun sequence.
ACCESSION   DASSWR010000563 DASSWR010000000
VERSION     DASSWR010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376863"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFO47_09415"
     CDS             complement(<1..1116)
                     /locus_tag="VFO47_09415"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET8788954.1"
                     /translation="MSGGVRAVDLIARKRDGGEVAGDELRDFVLAYAREELPDYQMAA
                     FLMAGYLRGFTRSETEALTGAMVASGDQLDLSRLSGPTVDKHSTGGVADGTTLVVGPL
                     AAALGMQVMKLSGRGLGHTGGTLDKLESIPGFRVGLGADELLDQVERIGLAVAAQTAD
                     LVPADKSIYALRDVTATVGNVALIASSVMSKKLAGGAASILLDVKTGSGAFMQETSEA
                     VELARLCVDIGRDAGRGTAALVTDMDQPLGEMVGNAVEVREAVEALRGERPGRFLDLC
                     LTLTGYMAALAGTAADPEAGRRDAERALERGDGLERFREFVAAQGGDPRVADDLSILP
                     QAPLTTEVEAPRDGWLEAVDAEAVGQVAAALGAGRRRK"
     gene            complement(1360..1602)
                     /locus_tag="VFO47_09420"
                     /pseudo
     CDS             complement(1360..1602)
                     /locus_tag="VFO47_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1704..2465)
                     /locus_tag="VFO47_09425"
     CDS             complement(1704..2465)
                     /locus_tag="VFO47_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8788955.1"
                     /translation="MGKVIALANQKGGVGKTTTAINLGAALAEQGRQVLLVDMDPQGA
                     LSVGLGLNPLALERTVYNLLMDPKADPGACIEATKQERLDLLPSNIDLAAAEVLLVGE
                     VAREQALSRVLRVLRKRYDYVLVDCPPSLGLLTVNALTAADGVLIPLECEYFALRGMA
                     LLMDTIEKVRERLNPDLEIVGILATMYDARTVHGREVLGRVEDAFGGRVFRTVIAKTI
                     RFAEAPVAGESILTYAGSSSGAVAYRELAKEVLAS"
BASE COUNT          318 a          946 c          934 g          372 t
ORIGIN      
        1 cttgcgccgc cgcccggcgc cgagggcggc cgcgacctgc cccaccgcct cggcgtcgac
       61 cgcttccagc cagccgtccc gcggcgcctc gacctcggtg gtgagcgggg cctggggcag
      121 gatcgagagg tcgtcggcga cccgcgggtc gccgccctgg gcggccacga actcgcggaa
      181 ccgctccagc ccgtccccgc gctccagggc ccgctcggcg tcgcggcggc ccgcctcggg
      241 gtcggcggcc gtgccggcga gggcggccat gtacccggta agggtcaagc agaggtcgag
      301 gaaccggccg gggcgctcgc ctcgcagggc ctcgaccgcc tcgcgcacct cgacggcgtt
      361 gccgaccatc tcccccagcg gctggtccat gtcggtgacc agggcggcgg tgccgcgccc
      421 ggcgtcccgg ccgatgtcga cgcagagccg ggccagctcg accgcctcgg aggtctcctg
      481 catgaacgcg cccgagccgg tcttgacgtc cagcaggatg ctggcggcgc cgccggcgag
      541 cttcttgctc atcacgctgg aggcgatcag ggcgacgttg ccgacggtgg ccgtcacgtc
      601 gcggagggcg tagatcgact tgtcggccgg gaccaggtcg gcggtctggg cggccacggc
      661 cagcccgatc cgctccacct ggtcgagcag ctcgtcggcg ccgaggccga cccggaagcc
      721 ggggatcgac tcgagcttgt cgagcgtgcc gccggtgtgg cccaggccgc gtccggacag
      781 cttcatcacc tgcatgccga gggcggcggc gagcgggccg acgacgaggg tcgtgccgtc
      841 ggcgacgccg ccggtggagt gcttgtcgac cgtcggcccg ctgaggcggc tcaggtcgag
      901 ctggtcaccg gaggcgacca tggccccggt cagggcctcg gtctcggacc gggtgaaccc
      961 gcgcaggtag ccggccatga ggaacgccgc catctggtag tcgggcagct cctcccgggc
     1021 gtaggccagg acgaaatcgc gcagctcgtc gccggcgacc tccccgccgt cccgcttgcg
     1081 ggcgatcagg tcgaccgccc gcaccccgcc gctcaccggc gcaccctccg atccaccaac
     1141 gtctcgtctg cgcccgccct gctcactgcg gcaccgtccg ggccccgggc gacccccctg
     1201 tggacaggcg cgggcacgtc gtccgcccgc cggctaccct cccgatccgg gggccccggc
     1261 aaccagggcc ggtgagctcg cgcgccccgc tcatctcgcg cgccccgctc gtctggcgcg
     1321 ttccgctcgt caggtgaggc gttcgaccag ggaggcgtct catctggtga ggcgcttgac
     1381 cagcggggcg tcggcgccgt cgcgttccag ctcggacagg acctgggcca gggcctcgcg
     1441 gacgatgcgg ccccgatcga cggcgacgcc gtgctcgcgg cggagcagga gacgcgcgtt
     1501 ctcgaggtcc atcagctcgt cggcggtgca gtagaaggtg accttctcgt cgtgccgctg
     1561 gcgcccgctc gggacccgct cggccggttc ccggtcgcgc cgctgggcct cctcggacgg
     1621 ctgcttggca tcccggcccc ggcccttctc gatcgaggtg acgcggaaca gctcgtcggc
     1681 cccgggcagg ctcgcacgac ccttcatgac gccagtacct ccttggccag ctcgcggtac
     1741 gcgaccgccc ccgacgacga gccggcatag gtcaggatgc tctcgcccgc caccggcgcc
     1801 tcggcgaacc ggatggtctt ggcgatgacg gtccggaaga cgcgaccccc gaaggcgtct
     1861 tcgaccctgc cgagcacttc gcgcccgtgg accgtccggg catcgtacat ggtggcgagg
     1921 atcccgacga tctccaggtc ggggttgagg cgctcccgca ccttctcgat cgtgtccatc
     1981 agcagggcca tgccgcgcag ggcgaagtac tcgcactcga gcgggatcag cacgccgtcg
     2041 gcggcggtga gggcgttgac ggtgagcagg ccgaggctgg gcgggcagtc gaccaggacg
     2101 tagtcgtagc gcttgcgcag gacccgcagg acccgcgaga gcgcctgctc gcgggccacc
     2161 tcccccacca gcaggacctc ggccgcggcc aggtcgatgt tggaggggag caggtcgagc
     2221 cgttcctgct tggtggcctc gatgcaggcg ccggggtcgg ccttggggtc catcagcagg
     2281 ttgtagacgg tgcgctcgag ggcgagaggg ttcaggccga gcccgaccga gagcgccccc
     2341 tgcgggtcca tgtcgacgag gagcacctgg cggccctgct cggccagggc ggcgccgagg
     2401 ttgatggcgg tggtggtctt gcccaccccg cccttctggt tcgccagggc gatgaccttc
     2461 cccatctccg gcacctccgg accgcccgcg gagcgctttg tcgtcacatt gctttgtctc
     2521 ttcggcgtcg aagcgtgaag tcgcgttggg tcttggttga tggaacgaca
//
LOCUS       DASSWR010000564         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_783907,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000564 DASSWR010000000
VERSION     DASSWR010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783907"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VFO47_09430"
     CDS             complement(<1..1384)
                     /locus_tag="VFO47_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788956.1"
                     /translation="MGEGLRAGMVVAVLVVASFLAPASSGSQGQEQAPTTVGTEVVGH
                     LAPPQRGGYGDVWAHKDVAYLGNLRQADCQPPNGVWAISLKDPAKPRALASFAKFPGS
                     DGEDVWVGSVRTKAFKGDLAAVGVQRCSRQVQGFTGLALYDVTNPARPKELGRLPSGV
                     VSGVHEVGVVQRPDGRVLALAAVPYSFNLSQGRLGDLRIIDITNPRRPREVADWDVRR
                     DGPAETRGQLAARRDVFDHSAWPFDRGNKVFASFWSAGVQFLDISDPASPRLIGQTPY
                     RPEDGYRGAHSGWFNEDETLFVQNDEAMMAVGSGSRRTWTFQRVFDTSELSRPRLLST
                     FATEAAVPGKDGRVGLDGVYSVHNAVVKGGLSYASWYSDGVRVVDLSDPARPREIASF
                     VPPPTSPRQTAATAQGGRRDMPVVWGVFPWKDLVMASDMNSGLWVIRVTADAKAANGG
                     GSGSQSGSGAA"
     gene            complement(1682..>2040)
                     /locus_tag="VFO47_09435"
     CDS             complement(1682..>2040)
                     /locus_tag="VFO47_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788957.1"
                     /translation="VEATLAAGVGATVILPDVLAAGGEGRAADDGSGAADPPEGQAAL
                     AALVSESATRVLLAAPPEAAEELEAAAIQAGIPSTRLGATGGDRLIVPGVLDLPLSRV
                     RDAYEGALPRALGEPA"
BASE COUNT          245 a          791 c          746 g          258 t
ORIGIN      
        1 gcgccgcccc tgacccgctc tgggagcccg acccgccgcc gttggccgcc ttggcgtcgg
       61 cggtgacccg gatgacccac aggccgctgt tcatgtcgct ggccatgacc aggtccttcc
      121 aggggaagac gccccagacc acgggcatgt cgcggcggcc gccctgggcg gtggccgccg
      181 tctggcgggg cgaggtcggc ggcggcacga acgaggcgat ctcgcgcggc ctggcggggt
      241 cggacaggtc gaccacccgg accccgtcgg agtaccagga ggcgtaggaa aggccgccct
      301 tgacgacggc gttgtggacc gagtagacgc cgtcgaggcc cacccgtccg tccttgccgg
      361 gcacggccgc ctcggtggcg aaggtcgaca gcagcctggg ccgggacagc tcgctggtgt
      421 cgaacacccg ctggaaggtc caggtgcgcc gggacccgct gccgaccgcc atcatcgcct
      481 cgtcgttctg gacgaacagg gtctcgtcct cgttgaacca gcccgagtgg gcgccgcggt
      541 agccgtcctc ggggcggtag ggggtctggc cgatcagccg gggcgaggcc gggtcgctga
      601 tgtccaggaa ctgcacgccc gccgaccaga aggaggcgaa caccttgttg cccctgtcga
      661 acggccaggc gctgtggtcg aagacgtctc gccgggcggc gagctggccc ctggtctcgg
      721 ccgggccgtc gcggcggacg tcccagtcgg cgacttcccg tgggcggcgc gggttggtga
      781 tgtcgatgat gcgcaggtcc ccgagccggc cctggctcag gttgaacgag tagggcacgg
      841 ccgccagggc cagcacccgc ccgtcgggcc gctgcaccac cccgacctcg tgcacgcccg
      901 agacgacccc gctggggagc cggcccagct ccttcggcct ggccgggttg gtcacgtcgt
      961 acagggccag cccggtgaac ccctgcacct gccgggagca gcgctgcacg ccgacggcgg
     1021 ccaggtcgcc cttgaacgcc ttggtcctga ccgaccccac ccagacgtcc tccccgtcgc
     1081 tcccggggaa cttggcgaac gaggccagcg cccgcggctt ggccgggtcc ttgaggctga
     1141 tcgcccacac cccgttgggc ggctggcagt ccgcctggcg caggttcccg agataggcca
     1201 cgtccttgtg cgcccagacg tccccgtagc ccccgcgctg cggcggcgcc agatgcccca
     1261 cgacctcggt gccgacggtg gtcggggcct gctcctgccc ctggctcccg gacgacgccg
     1321 gcgcgaggaa cgacgccacc accagcaccg ccacgaccat gccggcccgc agcccctcac
     1381 ccatgcggtc ctccggcagc cgacgtcctc aacccactgt cgcacgaccc gctgagattt
     1441 cgctgagcgc ccgccgggtt cgggcaggcc ggaaccccga cccgcggctg ccggtccccc
     1501 gccccgcggt ccccgccctc gcggtccccg tcccgcggtc cccgtcccgc ggtcacgccg
     1561 gaacccgacc cgccgttgtc gggtcccccg aggctactcc cggcgggttc gtcggtgcgt
     1621 cgggctgtcc acagcccccg tcaggcgggt cccccggccc gtcaggcaag ctcgccggcc
     1681 gtcaggcggg ctcgccgagg gcgcggggga gggcgccctc gtaggcgtcg cggacgcggg
     1741 acagggggag gtcgaggacg ccggggacga tcaggcggtc gccgccggtc gcgcccaggc
     1801 gggtgctggg gatgccggcc tggatggccg ccgcctcgag ctcctcggcc gcctcgggag
     1861 gggcggcgag gaggacccgg gtggccgact cgctgaccag cgcggccagc gccgcctggc
     1921 cctccggcgg gtcggcagct ccgctcccgt cgtccgcggc ccggccctcg cctccggccg
     1981 caaggacgtc cgggaggatc acggtggcgc cgaccccggc ggcgagggtc gcctcgacca
//
LOCUS       DASSWR010000565         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_614538,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000565 DASSWR010000000
VERSION     DASSWR010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614538"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFO47_09440"
     CDS             complement(<1..1472)
                     /locus_tag="VFO47_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8788958.1"
                     /translation="MGWGSGSAARCSAGSGPAAGCATTPSRVLGGGSGINGLVDGRPA
                     DDPAFQAEIGDAVDDLEAIDGVERVTGPWIDGEPVPGLVARDGQAVLIRVAMVTDLRG
                     AEHERAVERVVERLQAVDAPRVLVGGEERAREEFQHQAQEDLERGETLALPVMVVVLF
                     LVFRGFVAAVTPLLVALVAVAGALLILLGVSEVADISAYSVNVVTMLGLGLAVDYSLL
                     VVSRFREERGAGFELPEAIERTLATAGRTVAFSGLTVAAALCGLLAFAEPFLRSLAWG
                     GIGVVLVAMVAAVTLVPALLGLWGRRIRPAPAHLQSDHGTFYRLSRLVQRYAPAIVVL
                     VAALLVLLALPFRHARLENSGLESLPRSSESRQLFETVEARFQDGGTDPVVVVVESVP
                     GSPLVADFLRRVEELPGVARVEPRPVPPPLTVLDVIPEGTSEGPVATGLVERIRGLER
                     PAAVGVTGPAAFLVDYRDSLTGRLAYALGLIGLATFALLFL"
     gene            2034..>2662
                     /locus_tag="VFO47_09445"
     CDS             2034..>2662
                     /locus_tag="VFO47_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788959.1"
                     /translation="MPVSAEEAARRVDELRARLRDALYRYHVLSDPDISDAEYDELFR
                     ELERLEQDYPDLRTDDSPTQTVGAPQTGAFAPVRHRVPMLSLDNAFSTEELAAWGRRT
                     ERIVGDVPAYVCELKIDGVAVSLQYERGRLVRAATRGDGMVGDDITPNVRTIANVPQR
                     LTLDDPPPAFEVRGEIFFPSSGFEALNKQMTDEGKQVYANPRNAASGSLR"
BASE COUNT          403 a         1027 c          955 g          277 t
ORIGIN      
        1 aggaacagca gggcgaaggt ggccagcccg atcagcccga gcgcatacgc cagccgcccg
       61 gtcagcgagt cgcggtagtc gaccaggaag gcggcggggc cggtgacccc gacggcggcc
      121 ggccgctcca gcccgcggat ccgctcgacc agcccggtcg ccaccggccc ctcgctggtg
      181 ccctcgggga tgacgtcgag cacggtgagc ggcggcggga ccgggcgggg ctcgaccctg
      241 gccaccccgg gcagctcctc gacccggcgc aggaagtcgg cgaccagcgg ggagccgggg
      301 accgactcga ccacgaccac caccgggtcg gtgccgccgt cctggaagcg ggcctcgacc
      361 gtctcgaaca gctggcgcga ctccgaggag cggggcagcg actccaggcc cgagttctcc
      421 agccgggcgt ggcggaaggg cagggccagc agcaccagca gggcggcgac caggaccacg
      481 atcgccgggg cgtagcgctg caccagccgc gacagccggt agaacgtccc atggtcggac
      541 tggaggtggg ccggggccgg ccggatccgc cgcccccaga ggccgagcag ggccgggacc
      601 agggtgacgg cggccaccat ggccaccagc accaccccga tcccgcccca ggccagcgag
      661 cgcaggaacg gctcggcgaa ggcgagcagc ccgcacaggg cggcggccac ggtcagcccg
      721 gagaaggcca ccgtccggcc ggccgtggcc agggtgcgct cgatcgcctc ggggagctcg
      781 aacccggccc cccgctcctc gcggaagcgg ctgaccacca gcagggagta gtcgacggcc
      841 aggcccaggc cgagcatggt gacgacgttg accgagtagg cggagatgtc ggcgacctcg
      901 ctcaccccga gcaggatcag cagcgccccg gccacggcca ccagcgccac cagcaggggg
      961 gtgacggcgg ccacgaaccc ccggaacacg aggaacagca ccaccaccat caccggcagg
     1021 gccagggtct cgccccgttc caggtcctcc tgggcctggt gctggaactc ctcgcgggcc
     1081 cgttcctcgc cgccgaccag cacccggggc gcgtcgacgg cctggagccg ctccaccacc
     1141 cgctccacgg cccgctcgtg ctcggccccg cgcaggtcgg tcaccatcgc cacccggatc
     1201 agcaccgcct gcccgtcccg cgcgaccagg ccgggcaccg gctctccgtc gatccagggg
     1261 ccggtgaccc gctcgacccc gtcgatggcc tccaggtcgt cgacggcgtc gccgatctcg
     1321 gcctggaacg ccgggtcgtc ggccgggcgc ccgtcgacca gcccgttgat ccccgacccg
     1381 ccgcccagga cccggctcgg cgtcgtcgcg cagcccgctg ccggtcccga gccggccgaa
     1441 cacctcgccg ccgaccccga gccccacccc aaggaccagc acccaggcga gcgccaccgg
     1501 cacccgccgc cggtatacga accgacccaa ggacgcgaac acgacgccca gcatatggcg
     1561 acccgcgcgg gttcgccccc ggtggaacac cattcccttg ggggagggcc ggcgccgggg
     1621 agctgggatc agccctggtc gagcgcgccg ggcgttgccg acgccgtaga gcctgccgtc
     1681 ctggacgcgg tagtcgacgc cgaccagcga ctggtcgccg gccaggccct tgaccttgcc
     1741 gatgccccgg gtccggccgg gcgtggcggc gtcgaaccgg accagccggc cgtcggtggt
     1801 cagcccgacc acctgcaggc tccggcggtc gtcgcgccgt acggcgtcgt cggagcggta
     1861 caggtcgtcg agctggcggc cattggcgac ggtggggacc gcgagcaggc tcacggcgca
     1921 cagcagcagc aggccccgtc ggatcacccg ggctgtgggc ggcggggggc caatcgtggc
     1981 gcgggtgggc gatactggat gcccaggatg agcgccgagg cgaaggggga cgggtgccag
     2041 tttcggccga ggaggctgcc aggcgggtcg acgaactgcg ggcgcgcctg cgggacgcgc
     2101 tgtaccgcta ccacgtgctc agcgaccccg acatctccga cgccgagtac gacgagctgt
     2161 tccgcgagct ggagcgcctg gagcaggact accccgacct gcgcaccgac gactcgccca
     2221 cccagacggt gggagcgccc cagacggggg cgttcgcgcc cgtccgtcac cgggtgccga
     2281 tgctgtcgct ggacaacgcc ttctcgaccg aggagctggc cgcctggggc cggcgcaccg
     2341 agcgcatcgt cggcgacgtc cccgcctatg tctgcgagct caagatcgac ggggtggccg
     2401 tctccctcca gtacgagcgg ggccggctgg tccgggccgc cacccggggc gacggcatgg
     2461 tcggggacga catcaccccc aacgtgcgca ccatcgccaa cgtgccccag cgcctgaccc
     2521 tcgacgaccc gccgcccgcc ttcgaggtcc ggggagagat cttcttcccc tcctcggggt
     2581 tcgaggcgct caacaagcag atgaccgacg agggcaagca ggtctacgcc aacccccgca
     2641 acgccgcctc gggctccctg cg
//
LOCUS       DASSWR010000566         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1377004, whole genome shotgun sequence.
ACCESSION   DASSWR010000566 DASSWR010000000
VERSION     DASSWR010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377004"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFO47_09450"
     CDS             complement(<1..1284)
                     /locus_tag="VFO47_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8788960.1"
                     /translation="MDVRPRPDARDDAELVRAATAGDRGAFAAIYDRYADRLHDFCWS
                     LLRDRDEAADATQDAFLVAAERLGQLRDPERLRPWLYAVARSQAFRRARARSRVAPEE
                     EMTELPDAASGPEQAAERSDLRELVWNAAAGLSERDRALLDLHLRHGLEGAELGEAIG
                     VEPGHAYVLLNRLRAQVERSLGALLVARLGRDDCPDLATILTGWDGRFSPLIRKRVAR
                     HVDTCEICAERRRTVVSPLALLAAVPPMPAPADLRGRVLERIQLTGGSGSGGDGSGPG
                     GDGGASPGGPGSGSPGGPGSGSPGGPGPAAPGVSPAVAGSPGVSPAAAGAGAAGLGPP
                     GVGQPDPPLAGPPEGERSGPGGSGASGIHSPRRDSSRRRWAVVGLVAALLVAVATGVG
                     LGWERAPGPSSPADDQGPIVAGAATSVATSTPPTTV"
     gene            1390..1929
                     /locus_tag="VFO47_09455"
     CDS             1390..1929
                     /locus_tag="VFO47_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET8788961.1"
                     /translation="MDGIDPGDELLDVVDEHDRVIGQATRREVRRRRLLHRFSSVLCR
                     DPAGRVYVHRRTDGKDVYPGMYDMTAGGVLAAGETYLEGARRELAEELGVVGPEPRFL
                     FRHRYRGQENPSWSALFEVTWDGPVHPQASEIAWGAFLTLDELAARLEEWTFCPDGLE
                     IFRRWQARTGVATSRPKRR"
     gene            complement(1904..>2160)
                     /locus_tag="VFO47_09460"
     CDS             complement(1904..>2160)
                     /locus_tag="VFO47_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788962.1"
                     /translation="RGLMADAGFRRSDARAHSETLPLPSPEEFLWQYVSSTPLATVVT
                     DLDDRARSAVARDVVASWRPFTRQGRLLLALGFSVASGEK"
BASE COUNT          268 a          812 c          843 g          237 t
ORIGIN      
        1 gacggtcgtc gggggcgtgc tggtcgcgac cgacgtggcc gccccggcca cgatcgggcc
       61 ctggtcgtcg gcagggctgg aggggcccgg ggcccgctcc catcccagcc cgaccccggt
      121 cgcgaccgcg accagcagcg cggccaccag cccgacgacc gcccagcgac gccgggagct
      181 gtcgcgccgg gggctgtgga tacccgacgc ccccgacccg ccgggtccgc tacgctcccc
      241 ctccgggggg cccgccaacg gcgggtcggg ttgccccacg cccggagggc ccagccccgc
      301 cgcccctgcg cccgcggccg ccgggctcac gcccggggaa cccgcgaccg ccgggctcac
      361 gcccggtgcc gcgggacccg ggccgcccgg cgaccccgaa cccgggccgc ccggcgaccc
      421 cgaccccggg ccgcccggag acgcaccccc gtcaccgccc ggccccgacc cgtcgccgcc
      481 cgatcccgac ccaccggtga gctgaatgcg ctccaggacc ctcccccgga ggtcggccgg
      541 ggcgggcatg ggcgggacgg cggccaggag ggccaggggg ctgacgacgg tgcggcgacg
      601 ttcggcgcag atctcgcagg tgtcgacgtg gcgggcgacc cgcttgcgga tgagggggga
      661 gaagcggccg tcccagccgg tcaggatggt ggccaggtcg gggcagtcgt cgcggcccag
      721 gcgggccacg aggagggcgc cgagggaacg ctcgacctgg gcgcggaggc ggttgaggag
      781 gacgtaggcg tggccgggct cgacgccgat ggcctcgccg agctcggcgc cctcgaggcc
      841 gtggcggagg tggaggtcga gcagggcgcg gtcgcgctcg gacaggccgg cggcggcgtt
      901 ccagaccagc tcgcgcaggt ccgaccgctc ggcggcctgc tcgggaccgc tggcggcgtc
      961 gggcagctcg gtcatctcct cctcgggcgc gacgcgcgat cgggcccggg cccggcggaa
     1021 ggcctggctg cgcgccaccg cgtacagcca gggacgcagc cgctccgggt cgcggagctg
     1081 gccgaggcgc tcggccgcga ccaggaacgc gtcctgggtg gcgtcggccg cctcgtcccg
     1141 gtcgcgcagg agcgaccagc agaagtcgtg cagccggtcg gcgtagcggt cgtagatcgc
     1201 ggcgaaggcg ccgcggtcgc cggccgtcgc cgcccgcacg agctcggcgt cgtcgcgcgc
     1261 gtcgggacga ggtcggacgt ccattcccct ccataccatg gcccgggcag ggccgttcgg
     1321 caccatgagc ccggcggccg ccgcagcctt acgcttttcc ggaggtatct tccctcacga
     1381 cccgagaagg tggacggcat cgaccctggc gacgagctgc tggacgtggt cgacgagcat
     1441 gaccgggtga tcgggcaggc gacccggcgc gaggtccggc gccggcggct gctgcaccgg
     1501 ttctcctcgg tgctctgccg cgaccccgcc gggcgggtgt acgtgcaccg gcgcaccgac
     1561 ggcaaggacg tctacccggg gatgtacgac atgacggccg gcggggtcct ggccgccggc
     1621 gagacctacc tggagggggc ccggcgcgag ctggccgagg agctgggcgt ggtcgggccg
     1681 gagccgcggt tcctgttccg ccaccgctac cgcggccagg agaaccccag ctggagcgcc
     1741 ctgttcgagg tcacctggga cggccccgtc caccctcagg cgagcgagat cgcctggggc
     1801 gccttcctca ccctcgacga gctggcggcc cgcctggagg aatggacgtt ctgccccgac
     1861 ggcctggaga tcttccgccg ctggcaggcc cggacggggg tcgctacttc tcgcccgaag
     1921 cgacgctgaa gccgagcgcc aggagcaacc tgccttgccg cgtgaacggt cgccaggagg
     1981 cgacgacgtc ccgcgcgacc gcgctgcgag cgcggtcgtc gaggtccgtg accacggtcg
     2041 ccagcggcgt cgaagacacg tactgccaca ggaactcctc cggcgacggc agcgggagcg
     2101 tctcgctgtg ggcgcgcgcg tcggatcggc ggaagccggc gtcggccatg aggccccgaa
//
LOCUS       DASSWR010000567         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1092785, whole genome shotgun sequence.
ACCESSION   DASSWR010000567 DASSWR010000000
VERSION     DASSWR010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092785"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..696
                     /locus_tag="VFO47_09465"
     CDS             283..696
                     /locus_tag="VFO47_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788963.1"
                     /translation="MTEEVRMARGAALATAAVAPVALGIAWWAAGPKGLLGAAFGLAL
                     AVAFFSATLLAVGAAGRVQPELMLPAAVITFIFKMTVIGLLLFLLRDTTAFDREAFAL
                     SLVAGTVVYLAAEIRFAMRAKIPYVVTDENKAGSR"
     gene            712..927
                     /locus_tag="VFO47_09470"
     CDS             712..927
                     /locus_tag="VFO47_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET8788964.1"
                     /translation="MSEPEPPREPPGRGAEMVWSIIGTLIAGVLAWGGIGWLLDRWLG
                     TSYLVAVGILVGMAGAFYLIIKRYGSS"
     gene            938..1741
                     /gene="atpB"
                     /locus_tag="VFO47_09475"
     CDS             938..1741
                     /gene="atpB"
                     /locus_tag="VFO47_09475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET8788965.1"
                     /translation="MTAATLPLAQREGFHAPGIEIFEWPDIVHLEVAGIDLSINRVVL
                     QMFLVVLLVWGLFFFAFRKPQLVPSGLQNVMESIVDFVRNQIVLEVIGREGLRFLPYL
                     TTLFVFVFFGNLGGITPPVFFPLNGRMALPAIMAICSLLLFISVGIASQGFGHYFRNS
                     LFPPRIPWPVYVLLTPIEFISTFVVRPVTLAIRLLANMIAGHLILSVFFVATGYMLTS
                     MNISAVFGVGALIFSVALIAFELFVAGLQAYVFTILSAVYIAGALEPEH"
     gene            1775..2011
                     /gene="atpE"
                     /locus_tag="VFO47_09480"
     CDS             1775..2011
                     /gene="atpE"
                     /locus_tag="VFO47_09480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET8788966.1"
                     /translation="MNNILAAEETVSGSVSIIGYGLGTLGPGIGIGFLVGKTVEGISR
                     QPEAAGQVRTLMFIGLALVEALALFGFALAFVVR"
     gene            2024..2617
                     /gene="atpF"
                     /locus_tag="VFO47_09485"
     CDS             2024..2617
                     /gene="atpF"
                     /locus_tag="VFO47_09485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET8788967.1"
                     /translation="MHLAYLLAQEAEPAHEPNPILPAANELIWGTIAFFILLFVMYRT
                     VWPSVDKAFKDRRANIEGKLEKAEKDREEAEGLLEQYRRRLRDAEDETQRILEEARAS
                     ADRVRRALRDTAEAAAGRELDRARQAIRGERDQAIRQLRNEVGTLAVELATRVVGDSL
                     DRDRQLRLVDEYIDELGNQAQAGALTGRREEPDGDRG"
     gene            2601..3419
                     /gene="atpH"
                     /locus_tag="VFO47_09490"
     CDS             2601..3419
                     /gene="atpH"
                     /locus_tag="VFO47_09490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET8788968.1"
                     /translation="MATEVNQALATHAQGLFDQAAGRGQRAIDQVEADLEELARLLVH
                     QLRLRKALADPGLPPEPKRALLAELGQGRLDEASVELLATVATRQRVRLRDFPGLLAA
                     LAAMAAFTAADKAGQLEQLESELFGLATLVEREHGVRSALTNPGLPTENKRALVADLL
                     GGASKRTAGLADLLVELYEGHELDTVAKEWAEAAAARRNRVVAEVRSAVELDGRRRTR
                     LAEALARVIGRPVVLQVTVDEAILGSVVVRVGDELFDGSVRSRLEQAREALGVA"
BASE COUNT          469 a         1237 c         1211 g          537 t
ORIGIN      
        1 gcggtgacct tctggacggc gtcgccgagg gtgatggtgc aggcgatggc gccgccgatc
       61 tcgaccacgg gaccttggcg gtgccgcagc ggacctctgc gccgtggtct cacgtcctcg
      121 ttgtcggcgg cggtccggga ccgctgaagc ggggccgccg cgcttggcgc gacggcggtt
      181 gtggctggta gattcaccgt ccacttccac gccggggacg gtgccgcgac cagcggcggg
      241 tgcgccctgc gtggacgacc tgtctcggga ggcgggtggg cgatgaccga agaggtacgc
      301 atggcccgcg gggccgcgct ggcgaccgcg gccgtcgcgc cggtcgcgct cggcatcgcc
      361 tggtgggccg ccggccccaa gggcctgctc ggggccgcct tcgggctggc cctggccgtg
      421 gccttcttct cggccaccct gctcgccgtg ggggcggccg gacgggtcca gccggagctg
      481 atgctgccgg cggccgtgat caccttcatc ttcaagatga ccgtcatcgg cctgctgctg
      541 ttcctgctca gggacacgac cgccttcgac cgggaggcgt tcgccctgtc cctggtcgcc
      601 gggaccgtgg tctacctggc ggccgagatc cgcttcgcca tgcgggccaa gatcccctac
      661 gtcgtcaccg acgagaacaa ggcggggtcg cgatgataga ttcgcccgcc catgagcgaa
      721 cccgagccac ccagggagcc gcccggccgc ggcgccgaga tggtctggtc gatcatcggc
      781 acgctgatcg cgggcgtgct ggcctggggt ggcatcggct ggctgctcga ccgctggctc
      841 ggcaccagct acctggtcgc ggtcgggatc ctcgtcggca tggccggcgc cttctacctg
      901 atcatcaagc gctacggcag cagctgacgg gacgaccatg acggcagcga cgcttcccct
      961 cgcgcagcgg gagggattcc acgcccccgg catcgagatc ttcgagtggc cggacatcgt
     1021 ccacctcgag gtcgccggca tcgacctcag catcaaccgc gtggtcctgc agatgttcct
     1081 ggtcgtgctg ctggtctggg ggctgttctt cttcgccttc cgcaagcccc agctggtccc
     1141 gtccgggctc cagaacgtga tggagtcgat cgtcgacttt gttcggaacc agatcgtgct
     1201 cgaggtcatc ggccgcgagg ggctgcgctt cctgccctac ctgacgaccc tgttcgtgtt
     1261 cgtcttcttc ggcaacctgg gcggcatcac cccgccggtc ttcttcccgc tgaacggccg
     1321 catggccctg ccggccatca tggccatctg cagcctgctg ctgttcatca gcgtcgggat
     1381 cgccagccag ggcttcggcc actacttcag gaactcgctg ttcccgcccc gcatcccgtg
     1441 gccggtctac gtcctgctga cgccgatcga gttcatctcg accttcgtcg tccgcccggt
     1501 caccctggcc atccggctgc tggccaacat gatcgccggc cacctgatcc tgtcggtgtt
     1561 cttcgtggcc accggctaca tgctgacctc gatgaacatc agcgcggtgt tcggggtggg
     1621 ggcgctgatc ttctcggtcg ccctgatcgc cttcgagctg ttcgtggccg gcctgcaggc
     1681 ctacgtcttc acgatcctgt ccgccgtgta catcgccggg gcgttggaac ccgagcacta
     1741 gcaaaggagc aaccgcgaga tgagcctggc actgatgaac aacatcctgg ccgccgagga
     1801 gaccgtcagc ggcagtgtct cgatcatcgg ctacggcctg ggcacgctcg ggccgggcat
     1861 cggcatcggc ttcctggtcg gcaagaccgt cgagggcatc tcacgtcagc ccgaggcggc
     1921 cggccaggtc cggacgctga tgttcatcgg cctcgccctg gtcgaggccc tggccctgtt
     1981 cgggttcgcc ctggccttcg tcgtccggta acagggaggg cacgtgcatc tcgcctatct
     2041 cctggcccag gaagcggagc cggcccacga gccgaacccg atcctgccgg ccgccaacga
     2101 gctgatctgg ggcacgatcg ccttcttcat cctgctgttc gtcatgtacc ggacggtctg
     2161 gccgtcggtc gacaaggcgt tcaaggaccg ccgggccaac atcgagggca agctcgagaa
     2221 ggccgagaag gaccgcgagg aggccgaggg gctgctggag cagtaccgtc ggcggctgcg
     2281 cgacgccgag gacgagaccc agcggatcct tgaggaggcc cgggccagcg ccgaccgggt
     2341 ccgccgcgcg ctgcgggaca cggccgaggc cgcggccggc cgcgagctgg accgggcccg
     2401 ccaggccatc cggggcgagc gcgaccaggc catccgccag ctccgcaacg aggtcggcac
     2461 cctggccgtc gagctggcca cccgggtggt cggcgactcc cttgaccggg accgccagct
     2521 ccggctggtc gacgagtaca tcgacgagct cggcaaccag gcccaggccg gcgccctgac
     2581 cggccggcgc gaggaacccg atggcgaccg aggttaacca ggccctggcc acccacgccc
     2641 aggggctgtt cgaccaggcc gccgggcgcg gccagcgggc catcgaccag gtcgaggccg
     2701 acctcgagga gctcgcccgg ctgctcgtcc accagctgcg gctgcgcaag gccctggccg
     2761 accccgggct cccgcccgag cccaagcggg ccctgctggc cgagctcggc cagggccgcc
     2821 tggacgaggc cagcgtggag ctgctggcca ccgtggccac ccgccagcgg gtccggctgc
     2881 gggacttccc cgggctgctg gccgccctcg ccgccatggc cgccttcacc gccgccgaca
     2941 aggccggcca gctggagcag ctcgagagtg agctgttcgg cctggccacc ctggttgagc
     3001 gcgagcacgg ggtgcgctcg gccctgacca accccggcct gcccacagag aacaagcggg
     3061 ccctggtcgc cgacctgctc ggcggggcca gcaagcgcac cgccggcctg gccgacctgc
     3121 tggtcgagct gtacgagggc cacgagctgg acaccgtggc caaggagtgg gcggaggcgg
     3181 cggcggcccg ccgcaaccgg gtggtggccg aggtccgcag cgccgtcgag ctcgacggcc
     3241 gccgccggac ccggctggcc gaggcgctgg cccgcgtgat cggcaggccg gtcgtgctgc
     3301 aggtcaccgt ggacgaggct attttgggct cagtggtcgt ccgggtcgga gacgagctgt
     3361 tcgacggcag tgtccgcagc cgcctcgagc aggcccgcga ggccctcggg gtcgcctaag
     3421 tccaggcctg agaccagagc gaaaggcaca aacc
//
LOCUS       DASSWR010000568         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1038565, whole genome shotgun sequence.
ACCESSION   DASSWR010000568 DASSWR010000000
VERSION     DASSWR010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038565"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1079)
                     /locus_tag="VFO47_09495"
     CDS             complement(129..1079)
                     /locus_tag="VFO47_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET8788969.1"
                     /translation="MTGPLLAVGETMALLTAAEVGRLRHASSLTLGVAGAESNVAIGA
                     RRLGCPAAWVGRVGDDELGELVVSRIRAEGVDVGGVVRDPGAPTSLMLKERRTAAMVR
                     VHYYRRHGPGSRLQPADLDPAQVAAAAVLHVTGITPALSDSARATVDHAVELARSAGV
                     PVSFDLNYRSALWPPDQAAAVFRDLAARADLVFAGDDEAELLGLAGDPADLARGLADL
                     GAGHAVVKLGERGAVAFVAGQVHAVEPVPVPAVDPVGAGDAFVAGYLAETLAGRPVPE
                     RLATAAACGAFAVTVPGDWEGLPTRDELAALGARPGTVRR"
     gene            complement(1076..1714)
                     /locus_tag="VFO47_09500"
     CDS             complement(1076..1714)
                     /locus_tag="VFO47_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET8788970.1"
                     /translation="MSAFLERLAAVPVVAILRAAGAGRFLEVGRVLYEGGVRAIEVTL
                     TSEGALEAFGRLRAELPGDAVLGVGTVRSVGDGERAEAAGATYLVAPDFRPEVVEWAG
                     RRGLPVVPGALTPSEVAAAWAAGATAVKVFPVSAVGGPAYLKAIKAPLPEVPLVPTGG
                     VGIDDIGAYLAAGAVAVGIGSPLLGDAGDPGGDLGALADRARRAVAAAGDRR"
     gene            complement(1711..2676)
                     /locus_tag="VFO47_09505"
     CDS             complement(1711..2676)
                     /locus_tag="VFO47_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HET8788971.1"
                     /translation="MTAVVVITDCDHGGVDPERAALDGHDVELRVLDCRTPEEVAAQA
                     GDAEVLINQYVPITGAVLDALPRCRLVVRYGVGVDNVDVEAATERGVWVANVPDYGRD
                     EVADHTLALALSLLRGVVVLDRSVRDGTWDLEAARPLRRLSVLTWGVVGCGAIGRGVA
                     GRAAGLGMRVLGHDLPGVPSEPPIERVALEALLEGADVVSLHAALTPESHHLIGAAAL
                     ARMRPTAFLVNTARGGLVDAAALLAALDAGELAGAALDVLEGEPPDELGWRLARHPRV
                     VATPHAAWYSEEAFHTLKSEVAREALRVLEGGEPRSPVNRPAARR"
     gene            complement(2681..>2958)
                     /locus_tag="VFO47_09510"
     CDS             complement(2681..>2958)
                     /locus_tag="VFO47_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HET8788972.1"
                     /translation="TVPWTEEDPDADWRIEPDETTQDVLALYEGECARSREIVAAASS
                     LDEAVVHPRWKEELSRRWILTHMLEETARHVGHADILREQLDGATGE"
BASE COUNT          354 a         1208 c         1102 g          294 t
ORIGIN      
        1 aggagcccga acagcgccca ggcgagcagg accggccccc cggggccggc aaccccatct
       61 ccgatccgtc ccgccgtccc tggaccggcg tcaggtcccg gccctccgtg agggggccgg
      121 gacgatcctc agcgccgcac ggtgccgggg cgggcgccga gggcggccag ctcgtcgcgg
      181 gtggggaggc cctcccagtc gccggggacg gtgacggcga aggcgccgca ggcggcggcg
      241 gtggccaggc gctcggggac ggggcggccg gccagggtct cggccaggta gccggcgacg
      301 aaggcgtccc cggccccgac cgggtcgacg gccgggaccg ggaccggctc gacggcgtgg
      361 acctggccgg cgacgaaggc cacggcgccg cgctcgccca gcttgaccac ggcgtggccg
      421 gcgcccaggt cggccaggcc gcgggccagg tcggccgggt cgccggccag gccgagcagc
      481 tcggcctcgt cgtcgccggc gaacacgagg tcggcccggg cggccaggtc gcggaagacg
      541 gcggcggcct ggtcgggcgg ccacagggcc gagcggtagt tgaggtcgaa ggagaccggg
      601 accccggccg accgggccag ctcgacggcg tggtcgacgg tcgccctggc cgagtcgctc
      661 agggccgggg tgatgccggt cacgtgcagg accgcggcgg cggcgacctg ggccgggtcg
      721 aggtcggcgg gctgcaggcg cgacccgggg ccgtggcggc ggtagtagtg gacccggacc
      781 atggcggcgg tgcggcgctc cttgagcatc aggctggtcg gggcccccgg gtcgcggacc
      841 accccgccga cgtcgacgcc ctcggcccgg atcctggaca cgaccagctc gccgagctcg
      901 tcgtccccga cccgccccac ccaggcggcc gggcagccga gccgccgggc cccgatggcc
      961 acgttcgact ccgcccccgc caccccgagg gtcagggagc tggcgtgccg cagccgcccg
     1021 acctcggccg cggtcagcag ggccatggtc tcccccacgg ccagcagcgg cccggtcacc
     1081 gccggtcccc ggcggcggcg accgcccggc gggcccggtc ggccagggcg ccgaggtcgc
     1141 ccccggggtc gccggcgtcg ccgagcaggg gcgacccgat cccgaccgcg accgccccgg
     1201 cggccaggta ggcgccgatg tcgtcgatcc cgaccccgcc ggtgggcacc agcggcacct
     1261 cgggcagggg ggccttgatc gccttgaggt aggccggccc gcccaccgcc gacacgggga
     1321 acaccttgac cgcggtcgcc ccggccgccc acgccgccgc cacctcgctc ggcgtcagcg
     1381 ccccgggcac caccggcagc ccccgccgcc ccgcccactc caccacctcg ggccggaagt
     1441 ccggcgccac caggtaggtc gccccggcgg cctcggcccg ctccccatcg cccaccgacc
     1501 ggaccgtccc caccccgagc accgcgtccc cgggcagctc cgcccgcaac cttccgaagg
     1561 cctccagcgc cccctccgag gtgagcgtga cctcgatcgc ccgcacccca ccctcgtaca
     1621 gcacccgccc gacctccagg aaccgccccg ccccggccgc ccgcaggatc gccaccaccg
     1681 gcaccgcggc caggcgttcg aggaacgcgc tcatcggcgg gccgccgggc ggttgaccgg
     1741 cgagcggggc tcgccaccct ccaggacccg cagcgcctcc cgggccacct ccgacttcaa
     1801 ggtgtggaac gcctcctccg agtaccaggc ggcatggggg gtggccacga cccgcgggtg
     1861 ccgggccagc cgccagccga gctcgtcggg cggctccccc tcgagcacgt ccagggcggc
     1921 tccggccagc tccccggcgt ccagggcggc cagcagggcg gccgcgtcga ccagcccgcc
     1981 ccgggccgtg ttgaccagga acgccgtcgg ccgcatccgg gccagcgcgg ccgccccgat
     2041 caggtggtgc gactcggggg tgagggcggc gtgcagggag accacgtcgg ccccctccag
     2101 cagcgcctcc agcgccaccc gctcgatcgg cggctccgac ggcaccccgg gcaggtcgtg
     2161 gccgaggacc cgcatgccca gcccggccgc ccgtccggcg accccgcggc cgatggcccc
     2221 gcagccgacc accccccagg tgaggaccga caggcgccgc aggggccggg ccgcctccag
     2281 gtcccaggtg ccgtcccgga ccgaccggtc cagcacgacc accccgcgca gcagggacag
     2341 ggcgagggcc agggtgtggt cggcgacctc gtcgcggccg tagtcgggca cgttggccac
     2401 ccagacgccg cgctcggtgg ccgcctcgac gtcgacgttg tcgaccccga ccccgtagcg
     2461 gaccaccagc cggcaccgcg gcagcgcgtc cagcaccgcg ccggtgatcg gcacgtactg
     2521 gttgatgagg acctcggcgt cgccggcctg ggcggccacc tcctcggggg tgcggcagtc
     2581 cagcacccgc agctcgacgt cgtggccgtc cagcgccgcc cgctcggggt cgacaccgcc
     2641 gtggtcgcag tcggtgatca cgacgacggc ggtcatgtcc tcactccccg gtggcgccgt
     2701 cgagctgctc ccggaggatg tcggcgtggc cgacgtggcg ggccgtctcc tccagcatgt
     2761 gggtcaggat ccagcgccgg ctgagctcct ccttccagcg cgggtggacc acggcctcgt
     2821 ccagcgagga ggcggcggcc acgatctcgc ggctgcgggc gcactcgccc tcgtacaggg
     2881 ccaggacgtc ctgggtggtc tcgtccggct cgatccgcca gtcggcgtcc gggtcctcct
     2941 cggtccaggg gacggtga
//
LOCUS       DASSWR010000569         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_49546,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000569 DASSWR010000000
VERSION     DASSWR010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49546"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1233
                     /locus_tag="VFO47_09515"
     CDS             28..1233
                     /locus_tag="VFO47_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET8788973.1"
                     /translation="MPERPPTVGTPSLLRDINARTILEHIQRTGPVSRAQVARDSGLS
                     KPTVSLGLTALLDAGLVREVGRSSGRPGPSAVLYELNPSAGWVVGIDVGRRRVRAALA
                     DITGAVVARREERAQASSAHTLIGQIGAIAHGLAAEAGIGWDRVHHVTVGSPGVFEPT
                     RGTVTLAPNLPGWGRQGLVAALRDELGSRIGVENDVNLAAEGERWRGLGGDVRNFGFL
                     SVGTGVGMGLVLDGKLYRGASAAAGEVGYLPIGADPYDPHVRRRGAFEEAVNGAAVVR
                     AAGQAGLTGSLSAKKVFALARKGDPLAGRVVEDEARRLALGLAVVMAVVDLELVILGG
                     GVGGNADLLLPPIERELRTLSPLRPRLAVSALGENAVLQGAVATALEVAQSRLFDPAT
                     MLDRKELVV"
     gene            1281..>2414
                     /locus_tag="VFO47_09520"
     CDS             1281..>2414
                     /locus_tag="VFO47_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8788974.1"
                     /translation="MRRRVIGLLAIALLAAACTAGGGDDSKPAATLDPNAQHDPMTLT
                     VWSNFSGREGKVTTAVLESVKQKYPWMTVKHVQGKDDDAIVRAINGGTPPDVAISFTP
                     DNSARFCQTRAFQDLNPNLQADKIDKNAVWPAAIFSYTQYNNIQCALPMLTDAYGLYY
                     NVDLLQKAGFSEPPKTLSELTAMAKKLTEYNPDGSIKVAGFMPLFGVYQNTVNTLGHA
                     YAAKWYDSSGKPSLSTDPNWTKMLQWQKSLVDAIGYEKLQKFFGKLGGGDSEWSAQHG
                     FETGKIAMMIDGEWRNAFIAADKSKVSYATAPFPVDDAQPDLYGSGQVGGTIMGIPKG
                     VEHPAESWLLVKYMTTETSALVTLAKGLRNVPSTVESLKDPELN"
BASE COUNT          388 a          898 c          808 g          320 t
ORIGIN      
        1 gtttcctaac tataacaggg agaccccgtg cccgagcggc cgccaacggt gggaacgccc
       61 agcctgctgc gggacatcaa cgcccgcacg atcctggagc acatccagcg caccgggccg
      121 gtctcccggg cccaggtggc cagggacagc ggcctgtcca agccgacggt ctcgctcggg
      181 ctcaccgccc tgctcgacgc cggcctggtc cgggaggttg gccgctccag cggccggccc
      241 gggcccagcg ccgtgctgta cgagctcaac ccctcggccg gctgggtggt cggcatcgac
      301 gtcggccgcc ggcgggtccg cgccgccctg gccgacatca ccggcgccgt ggtcgcccgc
      361 cgtgaggagc gggcccaggc ctcaagcgcc cacaccctga tcggccagat cggcgcgatc
      421 gcccatgggc tggccgccga ggccggcatc ggctgggacc gggtgcacca tgtcaccgtc
      481 ggcagcccgg gcgtgttcga gccgactcgc ggcacggtca ccctggcccc caacctgccc
      541 ggctggggcc gccagggcct ggtggcggcg ctgcgggatg agctcggcag ccgcatcggg
      601 gtcgagaacg acgtgaacct ggccgccgaa ggcgagcgct ggcgcggcct tggcggggat
      661 gtccgcaact tcggcttcct ctcggtcggg accggcgtcg gcatgggcct ggtactggac
      721 ggcaagctgt accgcggggc cagcgccgcc gccggcgagg tcggctacct gcccatcggc
      781 gccgaccctt acgaccccca tgtccggcgc cgcggcgcgt tcgaggaggc ggtcaacggc
      841 gccgcggtgg tccgcgccgc cggccaggcc ggcctgaccg ggtcgctcag cgcgaagaag
      901 gtcttcgccc tggcccgtaa gggcgacccg ctggccgggc gggtggtcga ggacgaggcg
      961 cggcgcctcg cgctcggcct cgccgtggtg atggcggtgg tcgacctgga gctggtcatc
     1021 ctcggcggcg gcgtcggcgg caacgccgac ctgctgctcc cccccatcga acgggagctg
     1081 cgtacgctct ctccgctccg gccccgcctg gccgtctcgg cgctgggcga gaacgcggtg
     1141 ctgcagggcg ccgtggccac cgccctcgag gtcgcccaga gccggctgtt cgatcccgcg
     1201 accatgctcg accggaagga gctcgtggtc tgaagcccag caagtggcag caccgtccgc
     1261 cctcctgaag ggagcgcccc atgcgacgac gggtcatcgg tctcctggca atcgcactgc
     1321 tcgccgccgc ctgcaccgcc ggcggcggcg acgactccaa gcccgcggcc accctcgacc
     1381 ccaacgccca gcacgacccg atgaccctca ccgtctggag caacttctcc ggccgcgagg
     1441 gcaaggtgac caccgccgtc ctggagagcg tcaagcagaa gtacccgtgg atgacggtca
     1501 agcacgtcca gggcaaggac gacgacgcca tcgtgcgggc gatcaacggc ggcaccccgc
     1561 cggacgtcgc catctccttc acgcccgaca actcggccag gttctgccag acccgcgcct
     1621 tccaggacct caaccccaac ctgcaggccg acaagatcga caagaacgcc gtctggccgg
     1681 cggccatctt cagctacacc cagtacaaca acatccagtg cgccctgccg atgctcaccg
     1741 acgcctatgg cctctactac aacgtcgacc tgctccagaa ggccgggttc agtgagccgc
     1801 ccaagaccct gagcgagctc acggccatgg ccaagaagct gaccgaatac aaccccgacg
     1861 gcagcatcaa ggtggccggg ttcatgccgc tgttcggggt ctaccagaac accgtgaaca
     1921 ccctcggtca cgcctacgcc gccaagtggt acgacagctc cggcaagccc tcgctcagca
     1981 ccgaccccaa ctggaccaag atgctccagt ggcagaagtc gctggtcgac gccatcggct
     2041 acgagaagct gcagaagttc ttcgggaagc tcggcggcgg cgactcggag tggtctgccc
     2101 agcacggctt cgagaccggc aagatcgcca tgatgatcga cggcgagtgg cgcaacgcct
     2161 tcatcgcggc cgacaagtcg aaggtgagct acgccaccgc gcccttcccg gtcgacgacg
     2221 cccagcccga cctgtacggc tcggggcagg tcggcgggac catcatgggg attcccaagg
     2281 gcgtcgagca cccggccgag tcgtggctgc tggtcaagta catgacgacc gagacctcgg
     2341 ccctcgtgac cctggccaag gggctgcgga acgtgccgtc gacggtcgag tcgctcaaag
     2401 acccggagct caac
//
LOCUS       DASSWR010000570         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_247403,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000570 DASSWR010000000
VERSION     DASSWR010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247403"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFO47_09525"
     CDS             complement(<1..335)
                     /locus_tag="VFO47_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8788975.1"
                     /translation="MSAPAPPLPRYGEASLADLLPSLLAALDVPGFANPLGVEPLRRA
                     CLLVVDGLGWELLGANRRVAPVLAAAAERGRPVTTGFPSTTATSLASIGTGLPPGRHG
                     LVGYTIALPG"
     gene            413..811
                     /gene="msrB"
                     /locus_tag="VFO47_09530"
     CDS             413..811
                     /gene="msrB"
                     /locus_tag="VFO47_09530"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET8788976.1"
                     /translation="MAEVTRTEQEWRERLSPDQYRVLREKGTEPPFTGKYTYSKEEGV
                     YRCAACGNELFRSDAKFDSGTGWPSFTEPANATGVELHPDHSHGMVRTEVTCARCGSH
                     LGHVFPDGPAPTGQRYCINSLSLDLDAGGS"
     gene            874..2112
                     /locus_tag="VFO47_09535"
     CDS             874..2112
                     /locus_tag="VFO47_09535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8788977.1"
                     /translation="MAEDRPEQQQPTYNVLKTWAFDDWGLYGRGDELLARALARHPRV
                     GRVFHVQPPIDPAELRTRFRDPRWEQDMEGQLRRLKGITDGGVYLFTPTSEPDRREAA
                     LDATVAMLEREQAFAQPLVMLHGLAHPFAIDLASRLGGRGVTRVAQVRRDARAPYPVD
                     ADERQALEHVYKTQLSGADLVWAGTRELREEFTALNPRSVLHPVGADQGLAGGTPAAE
                     LAGLPRPLIIYAGSNRAYLNVGLIRGTADRLREATFVFVGPAADFLQPLVSGLRNVHV
                     IGPRPYRLLPDYLAAADVAIAPHQVAPATRAVVPEKLFGYLAAGLPVVTTEVAGAEEL
                     RRLVWIARDPSEFARAIQGALKRDRERRRALRQAAVAPLSWDAITAHTVASVDAVRAG
                     AELPAAPELKRPAIPGFLAR"
BASE COUNT          319 a          905 c          826 g          258 t
ORIGIN      
        1 ccgggcaggg cgatggtgta gccgaccagg ccgtggcggc ccggcggcag cccggtgccg
       61 atggaggcca ggctggtcgc cgtggtcgag gggaacccgg tggtgaccgg ccggccccgc
      121 tcggccgcgg cggccagcac gggggcgacc cggcggttgg cacccagcag ctcccagccg
      181 aggccgtcca ccaccagcag gcaggcccgg cgcagcggct ccaccccgag cgggttggcg
      241 aacccgggca cgtccagggc ggccagcagc gacggcagca ggtcggcgag ggaggcctcg
      301 ccgtagcggg gcagcggcgg ggccggggcg ctcatagcca gccagcctac taaggcccag
      361 ccggcgacgt ctctctagac tggagggaca actcgcggaa ggcggagatg acatggcaga
      421 ggtgacgagg accgagcagg agtggcggga gcggctcagc cccgaccagt accgggtgct
      481 gcgcgagaag ggcaccgagc cgccgttcac cggcaagtac acctactcca aggaggaggg
      541 cgtctaccgc tgcgcggcct gcggcaacga gctgttccgc tccgacgcca aattcgactc
      601 cggcaccggc tggccgagct tcaccgagcc ggccaacgcc accggggtgg agctccaccc
      661 cgaccacagc cacggcatgg tccgcaccga ggtcacctgc gcccgctgcg gctcccacct
      721 gggccacgtg ttccccgacg gccccgcccc gaccggccag cgctactgca tcaacagcct
      781 gtcgctggac ctggacgccg gcgggtcgta acgccgacgc tccctccggg gtcggttagg
      841 atcgccgtcc ccggagcaag ggaggaaccc aggttggcgg aggaccgccc cgagcagcag
      901 cagccgacct acaacgtgct caagacctgg gcgttcgacg actggggctt gtacggccgc
      961 ggggacgagc tgctggccag ggcgctggcc cgccacccgc gggtggggcg ggtgttccac
     1021 gtgcaaccgc cgatcgaccc ggccgagctg cgcacccgct tccgcgaccc ccgctgggag
     1081 caggacatgg agggccagct gcgccggctc aaggggatca ccgacggcgg ggtgtacctg
     1141 ttcaccccga ccagcgagcc cgaccggcgc gaggccgccc tggacgcgac cgtggccatg
     1201 ctggaacggg agcaggcctt cgcccagccg ctggtgatgc tgcacggcct ggcccatccc
     1261 ttcgccatcg acctggccag ccgcctcggc ggccgcggcg tgacccgggt ggcccaggtc
     1321 cgccgcgacg cccgcgcccc ctacccggtc gacgccgacg agcgccaggc cctcgagcac
     1381 gtctacaaga cccagctgtc cggggccgac ctggtctggg ccggcacccg tgagctgcgc
     1441 gaggagttca ccgccctcaa cccgcggtcc gtgctccacc cggtcggggc cgaccagggg
     1501 ctggccgggg gcacgcccgc ggccgagctg gccgggctgc cccggccgct gatcatctac
     1561 gccggctcga accgcgccta cctcaacgtc ggcctgatcc ggggcaccgc cgaccggctg
     1621 cgggaggcga ccttcgtgtt cgtgggcccg gccgccgact tcctccagcc gctggtctcg
     1681 ggcctccgca acgtgcacgt gatcggcccc cggccctacc ggctgctgcc cgactacctg
     1741 gccgcggccg acgtcgccat cgccccccac caggtggccc cggcgacccg ggcggtcgtg
     1801 cccgagaagc tgttcggcta cctggccgcc ggcctgccgg tggtcaccac cgaggtcgcc
     1861 ggggccgagg agctgcgccg cctggtctgg atcgccaggg acccctccga gttcgcccgg
     1921 gccatccagg gcgccctcaa gcgcgaccgg gagcgccgcc gtgccctgcg ccaggccgcc
     1981 gtcgcccccc tgtcgtggga cgcgatcacc gcccacaccg tggccagcgt cgacgccgtc
     2041 cgggcgggcg ccgagctgcc ggccgccccc gagctgaagc gccccgccat ccccgggttc
     2101 ctggcccgct gacccggccc cggagccctg gcgtccctgc ccaaccgggg cggagatggc
     2161 atgctggagc ttcgcgacca aggagcgcag cgtgcccgac gaccaggggc cttccgaccg
     2221 gccggagtgg ctgaccaagg ccaccacccc gaccacctgc taccggcatc cggaccggca
     2281 gaccgggctg cgctgctcgc gctgcgac
//
LOCUS       DASSWR010000571         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_981714,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000571 DASSWR010000000
VERSION     DASSWR010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981714"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFO47_09540"
     CDS             <1..526
                     /locus_tag="VFO47_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788978.1"
                     /translation="AATDPAALAGLVAGRLRQPPAVAPVRSGEVLAALDELLGRPEPV
                     VVPLPEPTLLPVESPEVVPDFVLLAADVMTWLAGLGETDLATWVVGGTWAEATARMAA
                     VVEAWSRWGPPGDGSMAAELEPRPRLDVVGHDEVGVISWTGVRAPAAELAAERPVGID
                     LAGDPEGDRQEVAT"
     gene            523..1134
                     /locus_tag="VFO47_09545"
     CDS             523..1134
                     /locus_tag="VFO47_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788979.1"
                     /translation="MNATELCRVLLVGSVAATDVPELGDPGTREEVRRRLGEAGCELV
                     YSAASERWLTRLDGPVPALEGHDPVLALGTAELAVLATCWLHLRFLPGEDGAGDGQDW
                     LDPDDLAELLGARLDGTGLPGVLERLARAGYLTLREGRVEAGPLLDTLDEPGTADQAR
                     ALVVRHQRLAHLRRRAAELDGGGGGSEGLKPGPPEDRGDDATD"
     gene            1121..>2099
                     /locus_tag="VFO47_09550"
     CDS             1121..>2099
                     /locus_tag="VFO47_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788980.1"
                     /translation="MPRIEEVQLVHWGSLRPDPIPLLVDGINVATGPNGSGKTCFLDG
                     IKLLLGVTSFAPGRSSHRYIFDGGPGGEPAQRAFLRATFSNPVLPGRGERLFAAADER
                     LRDADRVSVICLVTHDSRRYLVLPGLHRWGFEQPLADDLEAFLAAHPEQEWLGPRRYD
                     ELLDRTGITRALREVLALPQGAIDRTMDERPAGLLAKLLELTGERVLLDEVEAQRERA
                     GDARAAYLEAVEAARAEQDRLGALAGQAARHLEWAGLRERLDLLRDLARPAAEHRDVA
                     VRVEAARSERDAAAERIAADRRDLEELTGRLPALEARTAALDDQAKALVD"
BASE COUNT          228 a          797 c          836 g          238 t
ORIGIN      
        1 cgccgccacc gacccggcgg cgctggccgg gctggtcgcc ggccgcctgc ggcagccgcc
       61 ggccgtcgcg ccggtgcgga gcggcgaggt cctggccgcc ctcgacgagc tcctcggccg
      121 gcccgagccg gtggtggtgc cgctgcccga gccgaccctg cttccggtcg agtcgccgga
      181 ggtcgtgccc gacttcgtgc tgctggccgc cgacgtgatg acctggctgg ccgggctggg
      241 cgagaccgac ctggccacgt gggtggtcgg cgggacctgg gccgaggcca cggcccgcat
      301 ggccgcggtg gtcgaggcct ggtcgcgctg gggcccgccc ggggacggca gcatggccgc
      361 cgagctcgaa ccccggcccc ggctggacgt cgtcgggcac gacgaggtcg gggtcatctc
      421 ctggaccggt gtgcgcgccc ccgccgccga gctggccgcc gagcgcccgg tcgggatcga
      481 cctcgccggc gacccggagg gtgaccgcca ggaggtggcc acgtgaacgc gaccgagctc
      541 tgccgggtgc tgctggtggg gtcggtggcc gccaccgacg tgcccgagct gggcgacccc
      601 gggacccgcg aggaggtgcg gcggcgcctg ggcgaggccg gctgcgagct cgtctactcg
      661 gccgcctcgg agcgctggct gacccggctc gacggcccgg tgccggcgct ggagggccac
      721 gacccggtgc tcgccctcgg cacggccgag ctcgccgtgc tggccacctg ctggctccac
      781 ctgcgcttcc tgcccggcga ggacggggcg ggggatggcc aggactggct cgacccggac
      841 gacctggccg agctgctggg ggcgaggctg gacggcaccg gcctgccggg cgtgctcgag
      901 cggctcgccc gggccggcta cctgaccctg cgcgagggcc gggtggaggc cgggccgctg
      961 ctggacacgc tggacgagcc gggcaccgcc gaccaggccc gggccctggt cgtccgccac
     1021 cagcgcctgg cccacctgcg gcgccgggcc gcggagctcg acggtggggg cggcgggtct
     1081 gaggggctca agcctggccc cccagaggat cggggggacg atgccacgga ttgaggaggt
     1141 ccagctcgtc cactgggggt cgctgcggcc cgacccgatc cccctgctgg tcgacggcat
     1201 caacgtggcc accgggccca acggctcggg caagacctgc ttcctcgacg gcatcaagct
     1261 gctgctcggg gtgaccagct tcgcccccgg ccgcagctcc caccggtaca tcttcgacgg
     1321 cggccccggg ggcgagcccg cccagcgggc cttcctgcgg gccaccttca gcaacccggt
     1381 cctgcccggg cggggcgagc gcctgttcgc ggccgccgac gagcggctcc gcgacgccga
     1441 ccgggtgtcg gtgatctgcc tggtcaccca cgacagccgc cgctacctgg tcctgcccgg
     1501 cctgcaccgc tggggcttcg agcagccgct ggccgacgac ctggaggcgt tcctggccgc
     1561 ccacccggag caggaatggc tggggccgcg gcgctacgac gagctgctcg accggaccgg
     1621 catcacccgg gccctgcgcg aggtgctggc cctgccccag ggggcgatcg accggacgat
     1681 ggacgagcgg ccggccggcc tgctggccaa gctgctggag ctgaccggcg agcgggtcct
     1741 gctcgacgag gtcgaggccc agcgggaacg ggccggcgac gcccgcgccg cctacctgga
     1801 ggcggtcgag gcggcccggg ccgagcagga tcggctcggc gccctggccg gccaggccgc
     1861 ccgccacctg gaatgggccg ggctgcggga gcggctcgac ctcctccgcg acctggcccg
     1921 cccggcggcc gagcaccgcg acgtggccgt cagggtcgag gcggcccggt ccgagcggga
     1981 cgcggccgcc gagcggatcg ccgccgaccg gcgcgacctg gaggagctga ccggccggct
     2041 gccggcgctt gaggcgagga ccgccgccct ggacgaccag gccaaggcgc tggtcgacc
//
LOCUS       DASSWR010000572         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_219143,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000572 DASSWR010000000
VERSION     DASSWR010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219143"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..708
                     /locus_tag="VFO47_09555"
     CDS             196..708
                     /locus_tag="VFO47_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET8788981.1"
                     /translation="MMLRTQWYPFVDLRSAQDERAQDQLDRLFAQALGLHGQWQSATG
                     ASTRAWAPALDISEGKDAYRVTVELPGVKLDDLEITLEDGLLTIQGERHFANDSSEEQ
                     FHRVERSSGAFRRSITLPAHVEPDAVEALMEDGVLRILVPKAEEAKAKRIQVTPGGQM
                     AAIAASSATS"
     gene            839..1258
                     /locus_tag="VFO47_09560"
     CDS             839..1258
                     /locus_tag="VFO47_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8788982.1"
                     /translation="MADDDADIVRFVEIYLRLEGFEVVTARDGPDVLAKAVAVHPDLV
                     LLDVLMPGLDGYAVCAQIRADATLAAVPVIMVTGNYVLADVEAARRVGADDYLVKPFD
                     PAVLLDKAKTLLGRATVSPTLPGGRCGDGRRSRLPIR"
     gene            2004..2492
                     /locus_tag="VFO47_09565"
     CDS             2004..2492
                     /locus_tag="VFO47_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET8788983.1"
                     /translation="MSVVTEHLEQRGCVFEVIPHRQAYTSVDAARALGIEVGEVLKTL
                     ALRTGSGYALMVIPASHRLDLHLAREALGDNRARLASEEELGRDFPDYELGALPPLGV
                     LLDAQVYVDPEVLGHDTVTFAAGTQTESVKMQTQELFGSGGFTTMSLVKQPERGSDDR
                     IW"
BASE COUNT          426 a          864 c          886 g          422 t
ORIGIN      
        1 cgaccagcga cgggacccgc ccctccgggt accgattcaa cggtcgcgtg gcgagtatat
       61 gaacatcgtt cttgacagtt gaacagtgtt caatgtatgg tgggggtact cggcaagtgc
      121 acccgctgca cgcacccgga ctgcgttgtc cccgagccgg cggccgccga gcggcccaag
      181 gacgagggag atcagatgat gctcagaacc cagtggtacc cgttcgtgga cctgcgcagc
      241 gcgcaggacg agcgggccca ggaccagctg gacaggctgt tcgcccaggc acttggcctg
      301 cacggccagt ggcagagcgc gaccggggcc agcacccggg cctgggcgcc agcgctggac
      361 atctccgaag gcaaggacgc ctatcgggtg accgtggagc tgcccggggt caagctcgac
      421 gacctggaga tcacccttga ggacggcctg ttgaccatcc agggcgagcg gcacttcgcc
      481 aatgactcct ccgaggagca gttccaccga gtcgagcgct cctccggcgc cttccgccgc
      541 tcgatcaccc tgcccgccca tgtcgagcct gacgcggtcg aggctttgat ggaagacggc
      601 gtgctgcgca tcctggtgcc caaggccgaa gaagccaagg ccaagcgcat ccaggtcacc
      661 cccggcggcc agatggcggc catcgcagcc agctcggcca ccagctgacc atccagagtt
      721 gctcgcggaa catccaacgc atccgcctgg caacggcccc cggcggtacc gccgggggcc
      781 cccaccaagc tgccgaaccc gctcatgaga ggaccgcgcg ttggctgcta ccgtgctcgt
      841 ggccgacgat gacgccgaca tcgttcgctt cgtggagatt tatctccggc tggaaggctt
      901 tgaggtcgtc accgcccgcg acggccccga tgtgctcgcc aaggccgttg ccgtccaccc
      961 cgacctggtg ctgctcgatg tcctgatgcc cggcctcgac ggctacgcgg tctgcgccca
     1021 gatccgtgcc gatgcgaccc tggccgccgt cccggtgatc atggtcaccg gcaactacgt
     1081 cttggccgat gtagaggcgg cgcgccgggt tggggcggac gactacttgg tcaagccgtt
     1141 cgaccccgca gtcctgctcg acaaggccaa gaccttgctg gggcgggcga ccgtctcacc
     1201 cacccttcct ggtggccgct gcggcgacgg tcgccgcagc cgcttaccca tccgctgagg
     1261 acccggctac ggagcgggcg acagcgcccg cggcggacgc agcccacagc gacgcccggg
     1321 tcggctcgat ccgaatcgtt ctggagagag cgttgcatcg ggggtgcgca gttagcttcg
     1381 gcctgaccgg gatgtgtgaa gggacgggat gcccaacacg caacctcacc ccgccctacg
     1441 acacctccct ccagagcgcc tctgacctgg gtggttgtgc gggaacgtga ggcgctgcac
     1501 ggcgttgtga accacccgcg catcgatggt aaggatgggg tcgccggttc gattccggcg
     1561 gggggctcca caccaaggct gaccagcgga aaggctggcc agcgtccttc ccttcctggt
     1621 tggggccagc acgtagcagt cgggatgtgg cccaaggcca taacctctct cgatcagtca
     1681 tcggccctct gagcagcacc tttgctacca ggtagtctgg ctagcactca gtggactggg
     1741 gcacagcggt tgaagagcac atccctgtgt catggcatgt cctaaccggt agccaccggc
     1801 atcaccgatg cggatgtcta gagagaacgt cgctgagggt tcgatctttg cgccgtcaag
     1861 gtcgggcaac ggggttacga tcggcagtgc cgtcgatgcc gggtacggga tgccgggtac
     1921 ggtaggacca cagccgcgtg ggcggcgccg ggaaggcgtc gacctggccg ccgcgggacc
     1981 tcgcggccta ggaggtggca gcgatgtcgg tggtcaccga gcacctcgag cagcgaggct
     2041 gcgtcttcga ggtaatcccg catcgacagg cgtacacgtc cgtcgatgcg gcacgggccc
     2101 tcggcattga ggtcggcgag gtgctcaaga cgttggcgct gcggaccggg tccggttacg
     2161 cgctgatggt cattcccgcc tcgcatcggc tcgacctcca cctggcccgc gaggcgctcg
     2221 gtgacaatcg ggctcgtctg gccagcgagg aggagcttgg tcgtgacttc cccgactacg
     2281 agctgggtgc cctaccaccg ctcggggtgc tgctcgacgc gcaggtctat gtcgatccgg
     2341 aggtgctggg ccacgacacc gtgacgttcg cggccggcac ccagaccgag tcggtcaaga
     2401 tgcagaccca ggaactgttc gggtccgggg ggttcacgac catgtcgctg gtcaagcaac
     2461 cggaacgggg cagcgacgat cggatctggt aggcggcggg cggcccagca ggccgtgaca
     2521 gacgggggtc ggccagcgtc gccatggctc cttccgcacc ctggcttggc cggtacgacg
     2581 gccggctcgg gtgtttgt
//
LOCUS       DASSWR010000573         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_163307,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000573 DASSWR010000000
VERSION     DASSWR010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163307"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..494
                     /gene="raiA"
                     /locus_tag="VFO47_09570"
     CDS             78..494
                     /gene="raiA"
                     /locus_tag="VFO47_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET8788984.1"
                     /translation="MQVTVKGKNTGVPEKLRARAERKLAKLQRFDDHILSMDVEFSEE
                     RNPRVADPHRVEVTLTTKAGLIRAHANAVDPAAAVDQVVDRLERQVKKLKGRRVDRTQ
                     HADGVKALPVDDNETDQANVVYRRPDGDSGPGEQEP"
     gene            625..1278
                     /locus_tag="VFO47_09575"
                     /pseudo
     CDS             625..1278
                     /locus_tag="VFO47_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009996343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            1288..>2143
                     /locus_tag="VFO47_09580"
     CDS             1288..>2143
                     /locus_tag="VFO47_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8788985.1"
                     /translation="MATVLALDVGGTKLAAAVVDDAGRILGRGRVPSPTGTDPELLYE
                     AQLACAAAALRGADVTPGDLDGIGVAAAGPMVWPSGEVSPLNMPAWRGFPLRKRLAEE
                     FASDRVLIHNDAVGLTVGEHWKGAGAGTSNLLGITVSTGVGGGLILDGRLYHGFSGNA
                     GHVGHVVVELDGPPCACGGRGCLEAIASGPNSVRHALDDGWRPRPGVIADGVALAAAA
                     VAGDEIALRQVARAGRAVGTAIASCANLLDLEAAAIVGGFSQSGPAFWEPLQQAFSTH
                     AGFPFAAAC"
BASE COUNT          347 a          772 c          755 g          269 t
ORIGIN      
        1 tcccgttcgg gttgcaagac ccgatggggg gtatggccgg ctcgagatcg tatccggcaa
       61 ccgtatcggg agtgcccatg caggtgacgg tgaaggggaa gaacaccgga gtgcccgaga
      121 agctccgcgc tcgggccgag cgcaagctgg ccaagctcca gcggttcgac gaccacatcc
      181 tctcgatgga cgtggagttc tcggaggagc ggaacccgcg ggtcgccgac ccgcaccgcg
      241 tcgaggtcac cctgaccacc aaggccggcc tcatccgggc ccacgccaac gccgtcgacc
      301 ccgcggccgc cgtcgaccag gtcgtcgatc gcctcgagcg gcaggtgaag aagctcaagg
      361 gccggcgggt cgaccgcacc cagcacgccg acggggtcaa ggccctgcct gtggacgaca
      421 acgagactga ccaggccaac gtcgtgtacc gtcgccccga cggcgactcc gggcccggcg
      481 agcaggaacc gtgagcagcc agcaaccagc cgggggggac cgggggctca tgccgcagcc
      541 ggccgccccc ggcgggatca gggagtgaga ggggagagcg tggccgacga ccggcaggca
      601 cgaccgcggg gggcaggtga cgccatccgc gtgctgatcg tggacgacca cgcgttgttc
      661 cgccgtgggc tcgagatggt gctcgagcag gaggacgacg tcgaggttgt gggggaggcg
      721 ggtgacggcg ccgaggccat cgagaaggcc caggagctca tgcccgacgt ggtcctgatg
      781 gacgtccgca tgccgcggcg cagcggcatc gaggcgaccc agaccatcaa ggacctcatc
      841 ccgcacgcca agatcctgat gctgaccatc tcggatgagg aagccgacct gtacgacgcc
      901 atcaaggccg gcgccaacgg ctacctcctc aaggagatct cgatcgagga ggtcgccaac
      961 gcgatccgct cggtcgccca gggccagtcc ctgatctcgc cctccatggc ctccaagctc
     1021 ctcaacgaat tcgcgaccat ggtcaagcgg gccgacgaga agcagcaggt ccccaccccg
     1081 cggctcaccg agcgggagat ggaggtcctc aagctcgtcg ccaagggcca caacaaccgc
     1141 gacatcgcca aagagctcta catctccgag aacaccgtca agaaccacat ccgcaacatc
     1201 ctcgagaagc tgcacctgca ctcgaggatg gaggcggtgg tgtacgcggt ccgcgagaag
     1261 ctgctggaga tcaagtagcg gacgcggatg gccaccgtcc tggcacttga cgtcggcggg
     1321 accaagctgg ccgccgcggt ggttgatgac gccggccgga tcctggggcg gggccgggtc
     1381 ccctcgccga ccggcaccga ccccgagctg ctgtacgagg cgcagctggc ctgcgcggcc
     1441 gcggccctcc ggggcgccga cgtcaccccg ggcgacctgg acggcatcgg cgtggccgcc
     1501 gccgggccga tggtatggcc ctcgggcgag gtctcgccgc tcaacatgcc cgcctggcga
     1561 ggcttcccgc tgcgcaagcg cctggccgag gagttcgcca gcgaccgcgt gctcatccac
     1621 aacgacgcgg tcgggctgac ggtcggcgag cactggaagg gcgccggggc cggcacctcc
     1681 aacctgctcg gcatcaccgt ctccaccggc gtcggcggtg gcctcatcct ggacggccgc
     1741 ctctaccacg gcttcagcgg caacgccggc catgtcggcc acgttgtcgt cgagctcgac
     1801 ggcccgccct gcgcctgcgg cggccgcggc tgcctcgagg ccatcgcgtc cggccccaac
     1861 agcgtgcgtc acgccctcga cgacggctgg cggccgcggc ccggggtcat cgccgacggg
     1921 gtcgccctgg ccgcggcggc ggtggccggg gacgagatcg ccctgcgcca ggtggccagg
     1981 gccggccggg cggtggggac ggccatcgcc tcctgcgcca acctgctcga cctcgaggcg
     2041 gcggccatcg tcggcggctt ctcccagtcc ggcccggcct tctgggagcc gctccagcag
     2101 gcgttcagca cccacgccgg cttcccgttc gccgcggcct gcc
//
LOCUS       DASSWR010000574         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1151336, whole genome shotgun sequence.
ACCESSION   DASSWR010000574 DASSWR010000000
VERSION     DASSWR010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151336"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFO47_09585"
     CDS             complement(<1..714)
                     /locus_tag="VFO47_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET8788986.1"
                     /translation="MGGRLVLIVALITGVALVASVAALPLVVPAGGLARDTANKLVDV
                     PPLQETLPDPAQQSIIYAADGKTALATIWLDENRKVVGLKNIPKWVRNAVIAIEDDRF
                     YEHDGVDFRGIARAAVTDLRSRRIAQGGSTLTQQLVKLTITGNSRTLDRKLREAIYAV
                     ELERRYSKDEILQYYLNQAYFGEGVYGIATAAQHYFGNSSIKFVTLGQAASLAATIAA
                     PERYKPTGKKANKDRRNLVL"
     gene            860..1684
                     /locus_tag="VFO47_09590"
     CDS             860..1684
                     /locus_tag="VFO47_09590"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET8788987.1"
                     /translation="MQEAFRQFAYAFVDQLPNLLGALIVLVLTVVVARLVRRGMERAL
                     VRADAERHVRVVVATLAFYATVAIGLVVALSIGGVNLGVLIGSLGLATVALGFALQDI
                     VSNFSAGIVLLLEHPFTEGDHIAIADAEGEVEEIRVRATRLRAPDGQLVLVPNKLLFT
                     QVLTNATATMRRRVMVPLEVPYGQDVARARELVLAAAAGVEGVSDDPAPRLLTQNLGQ
                     DGRALRLTFWVDTRSDDPGRVRSEVLDEVERILEGFGGEDSPPNEPGTASGKEVRA"
     gene            complement(1661..>2792)
                     /locus_tag="VFO47_09595"
     CDS             complement(1661..>2792)
                     /locus_tag="VFO47_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET8788988.1"
                     /translation="AVVPGPGSPLGELAREARIIVCCGSGGVGKTSTAAAVALWAAET
                     GRRTCVLTIDPARRLAQALGLDLLSNTPRPVRARGLPARGPGSLDAMMLDMRRTFDEV
                     IERYARDTEQADRILANRFYQRLSSTLAGTQEYMAMEKLYELHESQRYDLLVVDTPPT
                     RSALDFLDAPDNLNELLDARAFRLLIAPAQRIGRGYLRGLNLATTAMTKMVRRVTGSE
                     LLAEVGEFFAAFEGMYDGFKERAGLVYDQLKHPSTAFVVVATPDGAALREAAYFAGRL
                     AADRMPLGALVVNRIHRAGPVPEVPAAARRRLAADGADGRLLADLLELHDGLEALAAA
                     EQRRVRDLLDRVPNLGVVQVPLLADDVHDLPGLRRLGAHLFA"
BASE COUNT          373 a          978 c         1039 g          402 t
ORIGIN      
        1 caggaccagg ttccgccgat ccttgttggc cttcttgccg gtgggcttgt accgctcggg
       61 ggcggcgatg gtcgccgcca gcgaggccgc ctggccgagg gtgacgaact tgatcgagct
      121 gttcccaaag tagtgctggg cggcggtggc gatgccgtag accccctcgc cgaagtaggc
      181 ctggttcagg tagtactgga ggatctcgtc cttgctgtag cgccgctcca gctcgaccgc
      241 gtagatggcc tcacgcagct tgcggtcgag ggtccgcgag ttgccggtga tggtgagctt
      301 gaccagctgc tgggtcaggg tgctgccgcc ctgggcgatc cggcgcgagc gcaggtcggt
      361 cacggccgcc cgggcgatgc cgcggaagtc gacgccgtcg tgctcgtaga accggtcgtc
      421 ctcgatcgcg atgacggcgt tgcggaccca cttggggatg ttcttgaggc cgaccacctt
      481 gcggttctcg tccagccaga tggtggccag ggcggtcttg ccgtcggcgg cgtagatgat
      541 cgactgctgg gccgggtcgg gcagagtctc ctggagcggc ggcacgtcga ccagcttgtt
      601 ggccgtgtcc ctggccaggc cgcccgccgg gacgaccagg gggagcgcgg ccacgctggc
      661 gacgagggcg acgccggtga tcagggcgac gatcagcacc agccgaccgc ccaccacgga
      721 ggctgcacga ccgcgggaga gacgggctgg cggcgcaggg atcggggcgt cggccatgct
      781 catcggatga tatagggtgg tcgatcggca ggggcaattc gacgcccgaa cgttatgagg
      841 aggccgcccg ctggaacgct tgcaggaggc gttccgccag ttcgcctacg ccttcgtcga
      901 tcagctgccg aacctgctgg gcgcgctgat cgtcctcgtg ctcaccgtgg tggtggcccg
      961 gctggtgcgc cgggggatgg agcgggcgct ggtccgcgcc gacgccgagc gccacgtccg
     1021 ggtggtggtg gccaccctcg ccttctacgc cacggtggcg atcgggctgg tcgtcgccct
     1081 ctccatcggc ggggtcaacc tcggcgtcct catcggcagc ctggggctgg ccaccgtcgc
     1141 cctcggcttc gccctccagg acatcgtcag caacttcagc gccggcatcg tgctgctgct
     1201 ggagcacccc ttcaccgagg gcgaccacat cgccatcgcc gacgccgagg gcgaggtgga
     1261 ggagatccgg gtccgggcga cccggctgcg cgcccccgac ggccagctcg tcctggtccc
     1321 caacaagctg ctgttcaccc aggtcctgac caacgccacc gcgaccatgc gccgccgggt
     1381 catggtgccg ctggaggtcc cctacggcca ggacgtggcc agggctcggg agctggtgct
     1441 ggccgcggcg gccggggtcg agggggtcag cgacgacccc gcgccccgcc tgctcaccca
     1501 gaacctgggc caggacggcc gtgccctgcg cctcaccttc tgggtcgaca cccgcagcga
     1561 cgacccgggc cgggtccgca gcgaggtgct ggacgaggtg gagcggatcc tggaggggtt
     1621 cgggggggag gactcccccc cgaatgaacc agggacggcg tcaggcaaag aggtgcgcgc
     1681 ctaatctgcg cagccctggc aggtcatgca cgtcgtcggc gagcaggggg acctggacca
     1741 cgcccaggtt cgggaccctg tccagcaggt cgcggacgcg gcgttgctcg gccgcggcca
     1801 gcgcctccag gccgtcgtgc agctccagca ggtcggccag caggcggccg tcggccccat
     1861 cggcggccag gcggcggcgg gcggccgccg ggacctcggg gaccggcccc gcccggtgga
     1921 tgcggttcac gaccagcgcg cccagcggca tgcggtcggc ggccaggcgg ccggcgaagt
     1981 aggcggcctc gcgcagcgcc gccccgtcgg gcgtggccac caccacgaac gccgtgctcg
     2041 ggtgcttgag ctggtcgtag acgagcccgg cgcgctcctt gaagccgtcg tacatgccct
     2101 cgaaggcggc gaagaactcg ccgacctcgg ccagcagctc cgacccggtg acccgccgga
     2161 ccatcttggt catggccgtg gtggccaggt tcagcccgcg caggtagccg cgcccgatcc
     2221 gctgggccgg ggcgatcagc agccggaagg cgcgggcgtc cagcagctcg ttcaggttgt
     2281 cgggggcgtc gaggaagtcg agcgccgagc gggtcggcgg ggtgtcgacc accagcaggt
     2341 cgtaacgctg ggactcgtgc agctcgtaca gcttctccat ggccatgtac tcctgggtgc
     2401 cggccagggt ggaggacagc cgctggtaga accggttggc caggatgcgg tcggcctgct
     2461 cggtgtcgcg ggcgtagcgc tcgatcacct cgtcgaaggt gcggcgcatg tccagcatca
     2521 tcgcgtccag gctccccggc ccccgggccg gcagccccct ggcccggacc ggcctaggcg
     2581 tgttggagag caggtccagc ccgagggcct gggcgagccg ccgcgccggg tcgatggtga
     2641 gcacgcaggt ccgccgcccc gtctccgccg cccacagcgc caccgcggcc gcggtcgagg
     2701 tcttgcccac gccccccgac ccgcagcaga cgatgatccg cgcctcccgc gccagctccc
     2761 ccaacggcga cccgggcccg ggaacgaccg cc
//
LOCUS       DASSWR010000575         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_840447,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000575 DASSWR010000000
VERSION     DASSWR010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840447"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..991)
                     /locus_tag="VFO47_09600"
     CDS             complement(359..991)
                     /locus_tag="VFO47_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788989.1"
                     /translation="MTRVFLVDDHHFFLSGLRAELGGEFEIVGEAGEVDAAVDGIRRT
                     RPDVVLLDVHLPGGGGRAVLEAVRQTDQDIRFLALSVSDAAEDVIGVIRAGARGYVTK
                     TISPHDLAAAIRRVQSGDAVFSPRLAGFVLDAFHGDLTGVVDPELDQLTAREREVLRH
                     IARGFTYKEVARRLHLSVKTVETHVSSVLRKLQLSSRHELTRWASDRRLV"
     gene            complement(988..>2011)
                     /locus_tag="VFO47_09605"
     CDS             complement(988..>2011)
                     /locus_tag="VFO47_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8788990.1"
                     /translation="VEPDDDRARLHPVRRPAARQALALGLVVLGTMLLLRESGLWFGD
                     RLVWPIALAAAGSAVIWARSDDRDRARWARIPGNPIEAMFTGRVGMIRVVVGGLLVAS
                     GLGLGFANTGADLAAAGDVLLGVAITLIGLGLIFGPWATRLASQLAEERRGRIRSEAR
                     AEMAAHLHDSVLHTLALIQRADASPEVIGLARRQERELRAWLYRAPVPPEGRLRPAVE
                     EIATRVEQRHNVPVDVVVVGDAPLDEAARALVDACQEAAVNAARHSGAPLVSVYVEVE
                     PDELTGFVRDEGKGFDPEEVPTDRRGIADSIRGRIRRHGGTVTILSSPGEGTEVQLRL
                     PRSQSS"
BASE COUNT          286 a          728 c          755 g          242 t
ORIGIN      
        1 gcccagctcg gccgcgcgct tggtcgccgc ccacaggccg cggccccggc cgagcgccga
       61 gcggtcggcc gggacgtggt ggacggcggg gaacagggtc cggatgtccg ggacgtggag
      121 cgggatcgag gccgtcagct ccgcgatccc ggcgggccgc gaaaccacca cacgcagcgg
      181 taggggagcg accgggaggt cggccagtgc cagctcggcc tgctgctcca tcgcagttct
      241 ccgggctcgg gttcccacca cgggttcggt cgacggaccc gtctcagaca agcatgccga
      301 tcaacgctga atcaggcaat ctcgtacaag aaaatatgta gaaaccgtaa cgaacgcgct
      361 agaccaggcg gcggtcgctg gcccagcggg tcagctcgtg gcggctggac agctgcagct
      421 tgcgcagcac cgacgacacg tgggtctcga ccgtcttcac cgacaggtgc aggcggcgcg
      481 cgacctcctt gtaggtgaag ccccgggcga tgtggcgcag gacctcacgt tcgcgggcgg
      541 tgagctggtc cagctccggg tcgacgacgc cggtgaggtc gccgtggaag gcgtcgagca
      601 cgaacccggc caggcggggg gagaagacgg cgtcgccgct ctggacccgg cggatggcgg
      661 cggccaggtc gtgcggggag atggtcttgg tgacgtagcc gcgggcgccg gcccggatca
      721 ccccgatcac gtcctcggcc gcgtccgaca ccgacagggc caggaagcgg atgtcctggt
      781 cggtctggcg gaccgcctcc aggacggccc ggccgccgcc gccgggcagg tgcacgtcca
      841 gcagcaccac gtctggccgg gtgcgccgga tgccgtcgac ggccgcgtcc acctcgccgg
      901 cctcgcccac gatctcgaac tcgcctccca gctcggcccg gagccccgag aggaagaagt
      961 ggtggtcgtc gaccaaaaag acgcgtgtca cgatgactgg ctcctcggca ggcgcagctg
     1021 gacctcggtg ccctcgccgg ggctggagag gatggtgacg gtgccgccgt ggcggcggat
     1081 gcggccgcgg atcgagtcgg cgatgccgcg ccggtcggtg ggcacctcct cggggtcgaa
     1141 ccccttgccc tcgtcgcgca cgaacccggt gagctcgtcc ggctcgacct cgacgtagac
     1201 cgacaccagc ggcgctcccg agtgccgggc ggcgttgacg gccgcctcct ggcaggcgtc
     1261 caccagcgcc ctggccgcct cgtcgagtgg ggcgtcgccg acgaccacga cgtccacggg
     1321 cacgttgtgg cgctgctcga cccgggtggc gatctcctcg acggccggcc gcagccggcc
     1381 ctcgggcggc accggcgccc ggtacagcca ggcccgcagc tcccgctcct gccggcgggc
     1441 caggccgatg acctcgggag aggcgtcggc ccgctggatc agggccaggg tgtggagcac
     1501 cgagtcatgg aggtgggcgg ccatctcggc ccgggcctcg gagcggatcc ggccccggcg
     1561 ctcctcggcc agctggctgg ccagccgggt cgcccagggg ccgaagatca ggcccagccc
     1621 gatcagggtg atggccaccc ccagcaggac gtcgccggcc gcggccaggt cggccccggt
     1681 gttggcgaac ccgaggccga ggccgctggc caccagcagc ccgccgacca caacccggat
     1741 catgccgacc cggccggtga acatggcctc gatcgggttg ccggggatcc gggcccaccg
     1801 agcccggtcg cggtcgtcgc tgcgggccca gatgaccgcc gagccggccg cggccagggc
     1861 gatcggccag acgagccggt cgccgaacca caggccgctc tcgcgcagca gcagcatggt
     1921 gccgagcacg accagcccga gggcgagagc ctgtctggcc gccggccgcc ggaccgggtg
     1981 gagccgggcc cggtcgtcgt ccggctcgac c
//
LOCUS       DASSWR010000576         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_19599,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000576 DASSWR010000000
VERSION     DASSWR010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19599"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1450
                     /locus_tag="VFO47_09610"
     CDS             80..1450
                     /locus_tag="VFO47_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8788991.1"
                     /translation="MSLLVAGLLLAAAGCGGGGDEGGEGGDNAITFWLAEDVAQRVAA
                     IETIVKGFQDQSGVQVKVVAIAEDQLATQIQSASAGGTLPDVMGPLSLGFVQNLATDD
                     LADPDAANEVIDSLGRETFSERALSLVQADGKPMGVPSDTWTQLLVYRKDLFEKAGLE
                     APDTFDKILAAATKLKQPGQNGIVAATKAGESFTEQTFEYFALANGCQLTDDQGAIQL
                     TSPQCVATFKFYDDLIKGGSARGGQDVDTTRAAYLAGKAGMIVWSSFLLDELAGLRND
                     ALPTCEQCRSDKRFLSDNSGVVTAISGPDGQPTQFGEISNYVIIKDGNVDPAKQFVEY
                     MLSDGYVDWLAVTPEGRFPARSGTKDNPEEYNEAWSKLEVGVDSKAPLSELYGPEVLE
                     ALTKSTDSMNRWGFEQGQGRIVGALAAEQPIPKALAAMLDGQLTPDAAAKQAQADVEE
                     IAQSIE"
     gene            1492..2439
                     /locus_tag="VFO47_09615"
     CDS             1492..2439
                     /locus_tag="VFO47_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018468177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8788992.1"
                     /translation="MPRRGTLAQQEARAGYALLSPTLIVVLFMVVLPILWTIVIAFQS
                     LRLRNLRTRLFDFQFTLDNFSTVLTSPGFLDSLKVTLIYSVVGTFLSIALGLVAALVV
                     RKPFKGRTLVRASMLLPYVAPVVAVTFVWQIMLNPELGIVNAIGTDLLGWKESIPFLE
                     QRTGTIGIFGLELRVPLALTLLILYQAWRYFPFSFLFILARLQALPGELDEAARVDGA
                     TPLQRFWRITLPQLQGVIALLTVLRFIWTFNEFDDIYLLTQGGAGTEVISVRVYHYLT
                     GRGDIGAAAALSLVLALLLVVLLFLYFRFFVHREEAKAI"
     gene            2436..>2656
                     /locus_tag="VFO47_09620"
     CDS             2436..>2656
                     /locus_tag="VFO47_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8788993.1"
                     /translation="MSKTETGTGVAERSQAPKATARRPSRPRPPLSREVVETRILGVL
                     KWITIAFFLVATLFPFYYMVTLAVRTIEDL"
BASE COUNT          410 a          961 c          875 g          410 t
ORIGIN      
        1 cggctcgtgg tccggtctgg tgggaagcag gttgctggtc gaaagctggg agggaaacgc
       61 catgaagccc cgacgcctga tgagtctgct ggttgccggc ctgctgctgg cggccgccgg
      121 atgcggcggt ggtggcgacg aggggggcga gggcggggac aacgccatca ccttctggct
      181 cgccgaggac gtcgcccaac gggtcgccgc catcgagacg atcgtcaagg ggttccagga
      241 ccagagcggc gtccaggtca aggtcgtcgc catcgccgag gaccagctcg ccacccagat
      301 ccagtcggcc agcgccggcg gcaccctgcc cgacgtgatg ggccccttgt cgctcggctt
      361 cgtgcagaac ctggccaccg acgacctggc cgaccccgac gcggccaacg aggtgatcga
      421 cagcctgggc cgcgagacct tctcggagcg ggcgctcagc ctggtccagg ccgacggcaa
      481 gccgatgggg gtccccagcg acacctggac ccagctcctg gtctaccgca aggacctgtt
      541 cgagaaggcc gggctggagg ccccggacac cttcgacaag atcctggccg cggcgaccaa
      601 gctcaagcag ccgggccaga acgggatcgt cgccgccacc aaggccggcg agtccttcac
      661 cgagcagacc ttcgagtact tcgcgctggc caacggctgc cagctcaccg acgaccaggg
      721 cgcgatccag ctgacctcgc cccagtgcgt ggccaccttc aagttctacg acgacctgat
      781 caagggcggc tccgccaggg gcggccagga cgtcgacacc acccgcgccg cctacctggc
      841 cggcaaggcg gggatgatcg tgtggtcctc gttcctgctc gacgagctgg ccgggctgcg
      901 caacgacgcc ctcccgacct gcgagcagtg ccgctccgac aagcggttcc tgtccgacaa
      961 cagcggcgtg gtcaccgcca tctccggccc cgacggccag ccgacccagt tcggagagat
     1021 ctccaactac gtcatcatca aggacggcaa cgtcgacccg gccaagcagt tcgtcgagta
     1081 catgctgagc gacggctacg tcgactggct cgccgtcacc cccgagggcc ggttcccggc
     1141 ccgctccggc accaaggaca accccgagga gtacaacgag gcctggagca agctggaggt
     1201 cggggtggac agcaaggcgc cgctgtcgga gctgtacggg cccgaggtgc tggaggccct
     1261 gaccaagagc accgactcca tgaaccgctg gggcttcgag cagggccagg gcaggatcgt
     1321 cggggccctg gcggccgagc agccgatccc caaggcgctg gccgccatgc tcgacgggca
     1381 gctgaccccc gacgcggcgg ccaagcaggc ccaggccgac gtggaggaga tcgcccagtc
     1441 gatcgagtga cgcccaggcg gtgacgtgcc gggtcgggaa gggagaggcc catgccgagg
     1501 cgggggactc tggcccagca ggaggcgcgg gccgggtacg cgctgctgtc gccgaccctg
     1561 atcgtggtgc tgttcatggt cgtcctgccg atcctgtgga cgatcgtgat cgccttccag
     1621 agcctgcgcc tccgcaacct ccgcacccgg ctgttcgact tccagttcac cctggacaac
     1681 ttctccaccg tgctgacctc gcccgggttc ctggactcgc tcaaggtcac cctgatctac
     1741 agcgtcgtcg ggaccttcct gtcgatcgcc ctggggctgg tcgccgccct ggtcgtgcgc
     1801 aagccgttca agggccgcac cctcgtgcgg gcgtcgatgc tgctgccgta cgtggccccg
     1861 gtggtggcgg tcaccttcgt ctggcagatc atgctcaacc ccgagctcgg catcgtgaac
     1921 gccatcggca ccgacctgct cggctggaag gagagcatcc cgttcctgga gcaacgcacg
     1981 ggcacgatcg gcatcttcgg cctcgagctg cgggtgccgc tcgccctcac cctgctcatc
     2041 ctgtaccagg cctggcgcta cttccccttc tcgttcctgt tcatcctggc ccggctccag
     2101 gccctgcccg gcgagctcga cgaggcggcc cgggtcgacg gggccacccc gctgcagcgg
     2161 ttctggcgca tcaccctgcc ccagctccag ggcgtcatcg ccctgctcac ggtgctccgg
     2221 ttcatctgga ccttcaacga gttcgacgac atctacctgc tcacccaggg cggcgccggc
     2281 accgaggtaa tctcggtgcg ggtgtaccac tacctcaccg gccgcggcga catcggcgcc
     2341 gccgccgccc tctcgctggt gctggccctg ctgctggtgg tgctgctgtt cctctacttc
     2401 cgcttcttcg tccaccgcga ggaggcgaag gcgatatgag caagaccgag accggcaccg
     2461 gggtcgccga gcggtcccag gccccgaagg ccacggccag gcggccgtcc cggccccggc
     2521 cgccgctcag ccgcgaggtc gtcgagaccc gcatcctcgg ggtgctgaaa tggatcacga
     2581 tcgccttctt cctggtcgcg accctgttcc ccttctacta catggtcacg ctggccgtcc
     2641 gcacgatcga ggacct
//
LOCUS       DASSWR010000577         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1122863, whole genome shotgun sequence.
ACCESSION   DASSWR010000577 DASSWR010000000
VERSION     DASSWR010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122863"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFO47_09625"
     CDS             complement(<1..185)
                     /locus_tag="VFO47_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8788994.1"
                     /translation="MSTVASRPLPRRRLWWTVSDAAAITKRNLYRYLRVPPLLLFSTI
                     QPIMFVLLFTYVFGGAI"
     gene            complement(182..1198)
                     /locus_tag="VFO47_09630"
     CDS             complement(182..1198)
                     /locus_tag="VFO47_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8788995.1"
                     /translation="MSDAAIVTEGLRRSFGPIKAVDGVDLAAPAGTVLGLLGPNGAGK
                     TTIVRILTTLLTPDGGSARVAGFDVVRDAGALREVIGLAGQYAAVDENLTGRENLDLV
                     GRLYHLGRDEARRRADEMLERFDLSGAANRTVKTYSGGMRRRLDLGASLVGRPQVLFL
                     DEPTTGLDPRSRFSLWDVIRELVSDGTTLLLTTQYLEEADRLADHIAVIDGGRVIAEG
                     TTDQLKSRVGGDLLAVRVVDRARVAEAAEAVADLGSGAPQVVAEAGEVSLPVREGTAI
                     LAEVVRRLDAAGLDISDLALRRPTLDDVFLALTGHVAEVAPDGDEPEPPARGHGRRPA
                     RSSA"
     gene            complement(1320..1988)
                     /locus_tag="VFO47_09635"
     CDS             complement(1320..1988)
                     /locus_tag="VFO47_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788996.1"
                     /translation="MIGLVLADDHRVFAEGLGVMLDAQDDLAVLGVAHDSHQAIELAA
                     RHEPTVLLLDAHMPGPDLATTLRAVRASSPGTKVLMLSADTRRETIAEALEAGADGFL
                     AKDASSRQVAGAIRTLVEGKGSVVAAAEPASPPVRDPSVDLRVRTLSGREREILGLLA
                     NGWSNRRIAEECFLSLNTVRTHVQNVLVKLGVHSKLEAVAFALEHQVVAAGAGAPAWA
                     SSAR"
     gene            2403..3074
                     /locus_tag="VFO47_09640"
     CDS             2403..3074
                     /locus_tag="VFO47_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8788997.1"
                     /translation="MFLADCQPLFNEALEALITRDGTNEVVGQASAVQQIVASVPQLK
                     PDLYVIDAGLALSNRPTTVETILAELPDTKVILLAQDTDIDLLLHAIRAGAVGVVGKK
                     SGTQTVLRAVQAVLDGEGVVPRAMLPQLFRRLLDLGDRASDAPLNRLSPREREVLALL
                     GRGWSNAQIGRELYISPHTVRTHVQNILQKLEMHSKLEAATFAMQHELATRRPSATEV
                     GRQRG"
     gene            complement(3071..>4159)
                     /locus_tag="VFO47_09645"
     CDS             complement(3071..>4159)
                     /locus_tag="VFO47_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8788998.1"
                     /translation="RARYDSGDYEGALDRVLEAAGYGELRAEQARRRERGDVRQLGIG
                     LAVYVEVTGFGSEYGAVEVEPDGRVTARTGVSPHGQGHETAFAQLVAATLGVAADQVT
                     VVHSDTAALPRGDGTMGSRSLQVGGSAVAEAAGQVLEQARELAAHLLEAPAEDVAVFA
                     GEGIGVAGAPSTALTWAELATAAADPARRPPGMDEGLAAVVDFKMERSTFPFGAHLAV
                     CEVDTETGLARLARHLAVDDSGRILNAMLAEGQVHGGIAQGVAQALFEEVRYDEDGNC
                     LNGNLASYAMPSAADLPSFETQRTQTPTPLNPLGAKGIGESGTIGATPAVQNAVVDAL
                     AHLGVTHIDMPLTPERVWRAICAARGEP"
BASE COUNT          561 a         1489 c         1544 g          565 t
ORIGIN      
        1 tggatggcgc cgccgaacac gtaggtgaac agcagcacga acatgatcgg ctggatggtc
       61 gagaacagca gcaggggcgg cacccgcagg tagcggtaga ggttccgctt ggtgatggcg
      121 gcggcgtcgg acaccgtcca ccagaggcgg cggcggggca gggggcggct ggcgacggtg
      181 ctcatgcgct gctcctcgcc ggccgacggc catggccgcg ggccgggggc tcgggctcgt
      241 cgccgtcggg ggccacctcg gcgacgtggc cggtcagggc caggaacacg tcgtcgaggg
      301 tgggacggcg cagggccagg tcggagatgt ccagcccggc cgcgtccagg cgccggacca
      361 cctcggccag gatggcggtg ccctcccgga ccggcaggga gacctcgccc gcctcggcca
      421 cgacctgggg cgcgcccgag ccgaggtcgg ccacggcctc ggccgcctcg gccacccgcg
      481 cccggtcgac cacccgcacc gccagcaggt cgccaccgac ccgcgacttg agctggtcgg
      541 tggtgccctc ggcgatgacc cggccgccgt cgatcacggc gatgtggtcg gccagccggt
      601 cggcctcctc caggtactgg gtggtcagca gcagggtggt gccgtcgctg accagctcgc
      661 ggatcacgtc ccagagcgag aagcggctgc gcgggtcgag gccggtggtc ggctcgtcca
      721 ggaacagcac ctggggccgg ccgaccaggc tggcccccag gtcgaggcgg cggcgcatcc
      781 cgcccgagta ggtcttgacg gtgcggttgg cggcgccgga gaggtcgaac cgctccagca
      841 tctcgtcggc ccgccgccgg gcctcgtccc ggcccaggtg gtagaggcgg ccgaccaggt
      901 cgaggttctc gcggccggtg aggttctcgt cgacggccgc gtactggccg gccaggccga
      961 tgacctcgcg gagggcgccg gcgtcgcgga cgacgtcgaa gccggccacc cgggccgagc
     1021 cgccgtcagg ggtgagcagg gtggtgagga tccggacgat ggtggtcttg ccggcgccgt
     1081 tggggccgag caggccgagg acggtcccgg ccggggcggc caggtcgacc ccgtccaccg
     1141 ccttgatggg cccgaaggac ctgcgcaggc cctcggtgac gatcgctgcg tcgctcaacg
     1201 cgcctcctgt tgctgtcggc cacggtccat gacagcacaa aaggttgaac acttcaagct
     1261 taggcgcatg cgccgtgacc cggggggtgg tcacccgggt cacgggacgt gggtcaggcc
     1321 tacctggccg agctggccca ggcgggggcg ccggcgccgg cggccaccac ctggtgctcc
     1381 agggcgaacg cgaccgcctc cagcttggag tggacgccca gcttgacgag gacgttctgc
     1441 acgtgggtgc ggacggtgtt gagcgagagg aagcactctt cggcgatccg gcggttggac
     1501 cagccgttgg ccagcaggcc gaggatctcg cgctcgcgac ccgacagggt ccgcacccgc
     1561 aggtcgacgc tggggtcgcg gaccggcggg gaggccggct cggccgcggc gacgacgctg
     1621 cccttgccct cgaccagggt gcggatggcc ccggcgacct gacggctgga ggcgtccttg
     1681 gccaggaagc cgtcggctcc ggcctcgagc gcctcggcga tcgtctcccg ccgggtgtcg
     1741 gccgacagca tcagcacctt cgtcccgggc gaggacgccc tgacggcccg caaggtggtg
     1801 gccaggtcgg gacctggcat gtgggcgtcg agcagcagca cggtcggctc gtgcctggcc
     1861 gccagctcga tcgcctggtg gctgtcgtgc gccaccccga ggacggccag gtcgtcctgg
     1921 gcgtccaaca tcactccaag gccctcggcg aagaccctgt ggtcgtccgc cagaaccaac
     1981 ccgatcatgt tcgtcacgtt acggcctccc ccggcgcgcc tcagtcgctt cggcccgttc
     2041 tgcgaccgtc ttgtaaggcg tcgccggagc cgcaaaaggt acggccgaca cctcgtacgt
     2101 gaccgacccg tgatcggtac gtgatgatcc gcggcgggga ccggtccccc ggtctcgcgg
     2161 tgcgtcaggc ctacgacgct catcgcatgg ttctcatccg gagaactgag tacctgctgt
     2221 caccgagtcg ccgatggtcc ggccaaggct gtgacgtagg tgaagttgcg ccggttaccg
     2281 taatatccgg tactgcatcc ctcgtatccg ccgaaagggg cggtgcgaca agctgttggc
     2341 cacgacaagg acgtaggtca tggtggccgt tcgccggacg ccggaggaag tcgatggcac
     2401 gcgtgttcct cgctgactgc cagccgctgt tcaacgaggc gctggaagcg ctcatcaccc
     2461 gtgacggcac caacgaggtg gtcggccagg catcggccgt gcagcagatc gtcgcctcgg
     2521 tgccccagct caagcccgac ctgtacgtga tcgacgccgg cctggccctg agcaaccggc
     2581 cgaccaccgt cgagacgatc ctggccgagc tgcccgacac caaggtcatc ctgctcgccc
     2641 aggacaccga catcgacctg ctgctgcacg ccatccgggc cggcgcggtc ggggtggtcg
     2701 gcaagaagtc cggcacccag acggtgctgc gggccgtcca ggcggtgctg gacggggagg
     2761 gcgtggtccc gagggcgatg ctgccccagc tgttccgccg gctcctggac ttgggcgacc
     2821 gggcctccga cgcccccctg aaccggctgt ccccgcggga gcgggaggtg ctggccctgc
     2881 tcggccgcgg ctggagcaac gcccagatcg gccgcgagct ctacatcagc ccgcacacgg
     2941 tccgcaccca cgtgcagaac atcctccaga agctggagat gcactccaag ctggaggcgg
     3001 ccaccttcgc catgcagcac gagctggcca cccgccgccc gtcggcgacc gaggtcggcc
     3061 gccagcgcgg ctaaggctca ccccgggcgg cgcagatcgc ccgccagacc cgttcgggcg
     3121 tgagcggcat gtcgatgtgg gtcacgccca ggtgggccag ggcgtcgacc accgcgttct
     3181 ggacggccgg ggtggcgccg atcgtccccg actcgccgat ccccttggcg cccaacgggt
     3241 tgagcggggt cggggtctgg gtgcgctggg tctcgaacga gggcaggtcg gccgcgctcg
     3301 gcatggcgta gctggccagg ttgccgttca ggcagttgcc gtcctcgtcg tagcggacct
     3361 cctcgaacag ggcctgggcg accccctggg cgatcccgcc gtgcacctgc ccctcggcca
     3421 gcatggcgtt gaggatccgg ccggagtcgt cgacggccag atggcgcgcc agccgggcca
     3481 ggccggtctc ggtgtcgacc tcgcagacgg ccaggtgggc gccgaacggg aaggtgctgc
     3541 gctccatctt gaagtcgacg accgcggcca ggccctcgtc catgccgggc ggccggcggg
     3601 ccgggtcggc ggccgccgtg gccagctcgg cccaggtcag cgccgtcgac ggggcgcccg
     3661 cgaccccgat gccctcgccg gcgaacacgg ccacgtcctc ggccggcgcc tccagcaggt
     3721 gggcggccag ctcccgggcc tgctccagga cctggccggc cgcctcggcc acggcgctgc
     3781 cgccgacctg gagcgagcgc gagcccatgg tgccgtcgcc ccggggcagc gcggcggtgt
     3841 cggagtggac cacggtgacc tggtcggcgg cgaccccgag ggtggccgcg accagctggg
     3901 cgaaggcggt ctcgtggccc tggccgtgcg gggagacgcc cgtcctggcc gtgacccgcc
     3961 cgtcgggctc gacctcgacc gcgccgtact cggagccgaa cccggtcacc tcgacgtaca
     4021 ccgccaggcc gatgccgagc tggcggacgt cgccgcgctc gcgccgcctg gcctgctcgg
     4081 cccggagctc gccgtacccg gccgcctcca ggacccggtc gagggcgccc tcgtagtcgc
     4141 ccgagtcgta gcgggccct
//
LOCUS       DASSWR010000578         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_559228,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000578 DASSWR010000000
VERSION     DASSWR010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559228"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFO47_09650"
     CDS             complement(<1..374)
                     /locus_tag="VFO47_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET8788999.1"
                     /translation="MTQPPPAATIAIVGGRVVPVAGPPAEDGVVLVRDGRIEAVGRDV
                     RVPPGAARVDAGGKVVLPALVDAHVHLGVHEEAEGWAGQDTNELTDPVTPHVRALDAI
                     NPADLGFADALAGGVLTVNVNPG"
     gene            complement(371..868)
                     /locus_tag="VFO47_09655"
     CDS             complement(371..868)
                     /locus_tag="VFO47_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789000.1"
                     /translation="MEPNTPFYRRWSYLLAGVIGLAVVVALLGWRGFTEAGTSATTST
                     ATPVTTVAPPTTTQPETTVGPTSKPYDILWEELASEASTLRSEGFEAPPVWRIEWAFD
                     CSNFGDYGGGNFKITGDGDFQQEIQIEKRAVRYNGTTPVRRGGFGHLYIETVCEQWTV
                     RALSG"
     gene            1200..>2188
                     /locus_tag="VFO47_09660"
     CDS             1200..>2188
                     /locus_tag="VFO47_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8789001.1"
                     /translation="MTTTEYRQFIGGEWTGASGGATFEDLDPYSGEVVATAAAGTRED
                     ARRAVEAAQAAFPAWSQTPPAERQRIFLAAADILERRADEVVGLLARETGCVTYGFGM
                     FQMGFVPGLLRQAAALAYSPIGQILPADLPGAMAMGLRRPVGVVGAVFPWNAALILSA
                     RGIAAPLALGNTVVAKPSEESPFVGGLLWGEIFAEAGLPDGVLNIVTHAPGEAGPIGE
                     ELVEHPAVRRLNFTGSTATGRLLAEAAGRHLKRVVLELGGVNPLLVLDDADLDYAVDA
                     SAFGAYLHQGQICMSARRLLVDRRVADEVLDRLAAKTAGLPVGDPAQPGTVIGP"
BASE COUNT          270 a          821 c          810 g          287 t
ORIGIN      
        1 ccggggttga cgttgacggt caggaccccg ccggccagcg cgtcggcgaa gccgaggtcg
       61 gccgggttga tggcgtcaag ggcccgcacg tgcggggtca cggggtcggt gagctcgttg
      121 gtgtcctggc cggcccaccc ctcggcctcc tcgtggacgc ccaggtgcac gtgggcgtcg
      181 accagcgcgg ggaggacgac cttgccgccg gcgtccaccc gcgcggcacc ggggggtacg
      241 cgcacgtcgc ggccgacggc ctcgatgcgc ccgtcgcgga cgaggacgac cccgtcctcg
      301 gcgggcggcc cggccaccgg gacgacccgt ccgcccacga tggcgatcgt ggccgccggg
      361 ggtggctggg tcatccggac agggcccgga cggtccattg ctcgcagacg gtctcgatgt
      421 acaggtggcc gaagccgccc cggcggacgg gcgtggtgcc gttgtagcgg acggctctct
      481 tctcgatctg gatctcctgc tggaagtcgc cgtcgccggt gatcttgaag ttcccgccgc
      541 cgtagtcgcc gaagttgctg cagtcgaagg cccactcgat gcgccagacc gggggcgcct
      601 cgaagccctc gctccggagc gtgctcgcct cgctggcgag ctcctcccag aggatgtcgt
      661 agggcttgct ggtgggcccg accgtcgtct cgggctgggt ggtggtcggg ggggccacgg
      721 tggtgacggg cgtggcggtg ctggtggtgg cggacgtgcc cgcctcggtg aacccccgcc
      781 agccgagcag ggccacgacc acggccagcc cgatgacgcc ggccagaagg tacgaccagc
      841 ggcgatagaa gggggtgttg ggctccatct ccgccgagga tgctaaacga tatctcgggc
      901 aatcccgcaa ccgtgggtca ccacgacccg ccaccccgcc accggcaccg ccgccgaccc
      961 cgccaccgga gccgccggag gacgcccgca cccggccgac gagtccgtca gcagcaccgg
     1021 gacgatcacc ggacaccggg tcccacagct cgcggccggc ggcagggccc ggcggtggcg
     1081 aacagggccg ccccctcgcc gtcgtccagg tcgtcggccg tcgccgcctg atctccatgg
     1141 ggcgggaccg tggcacgcgg taggatccgc gatgtcgagg ccgaccaggg aggtcgtgga
     1201 tgaccaccac cgagtaccga cagttcatcg gcggcgagtg gaccggggcg agcggcgggg
     1261 cgaccttcga ggacctcgac ccgtactcgg gcgaggtcgt ggccacggcc gccgccggca
     1321 cccgcgagga cgcccgccgg gccgtggagg ccgcccaggc cgccttcccg gcctggtcgc
     1381 agacgccgcc ggccgagcgc cagcggatct tcctggccgc cgccgacatc ctggagcgcc
     1441 gcgccgacga ggttgtcggc ctgctggccc gcgagaccgg ctgcgtcacc tacgggttcg
     1501 gcatgttcca gatgggcttc gtgcccggcc tgctgcgcca ggcggccgcc ctggcctaca
     1561 gcccgatcgg ccagatcctc cccgccgacc tgcccggggc catggccatg gggctgcgcc
     1621 ggccggtcgg ggtggtcggc gccgtcttcc cctggaacgc cgccctgatc ctgtccgccc
     1681 gcggcatcgc cgccccgctg gcccttggca acacggtcgt ggccaagccg tcggaggagt
     1741 cgccgttcgt gggcgggctg ctctggggcg agatcttcgc cgaggccggg ctgcccgacg
     1801 gcgtcctcaa catcgtcacc cacgcccccg gcgaggccgg acccatcggc gaggagctgg
     1861 tcgagcaccc ggcggtccgg cggctcaact tcaccggctc caccgccacc ggccgcctcc
     1921 tggccgaggc cgccggccgg cacctcaagc gggtcgtgct cgagctcggc ggcgtcaacc
     1981 cgctgctggt gctggacgac gccgacctgg actacgccgt cgacgccagc gccttcgggg
     2041 cctacctgca ccaggggcag atctgcatgt cggccaggcg gctgctggtc gaccggcggg
     2101 tcgccgacga ggtcctggac cgcctggccg ccaagacggc cgggctcccg gtcggcgacc
     2161 cggcccagcc cggcaccgtc atcgggcc
//
LOCUS       DASSWR010000579         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_726739,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000579 DASSWR010000000
VERSION     DASSWR010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726739"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFO47_09665"
     CDS             complement(<1..262)
                     /locus_tag="VFO47_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8789002.1"
                     /translation="MIVLDTSGLLAALDADQRHHASAVQVLASDDGPFLLSPFVLAEL
                     DYLLLERVGVQAERALLDEVASGAYDLVPFSVAEVAAAAALVG"
     gene            complement(259..477)
                     /locus_tag="VFO47_09670"
     CDS             complement(259..477)
                     /locus_tag="VFO47_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET8789003.1"
                     /translation="MQKTTVYLPDALKRRLAQAAHRRGGPEAALIRAAIERLLEEEAR
                     PRPTLPLFRSGDPTLAERVDEALAGFGE"
     gene            complement(492..1205)
                     /locus_tag="VFO47_09675"
     CDS             complement(492..1205)
                     /locus_tag="VFO47_09675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8789004.1"
                     /translation="MSWEWQAGSWLRWARTPGHDSYWFFRDAFFDAIVPAAGRRTLEV
                     GCGEGRVTRDLVQRGHQVVALDISETLIASAAAANPFGDHVRGDAAALPFADASFDLV
                     VAYNSLMDVDDMPAAVHEASRVLEPGGRLGVCVTHPVADAGKFESKAPDAPFVIGGTY
                     LGRHRFEDTLERDGLQITFTGWRYSLEEYTRALEEAGLLIERLREPPAPPAALDARPG
                     YERWRRLPNFLHLRAVKPA"
     gene            complement(1216..2601)
                     /locus_tag="VFO47_09680"
     CDS             complement(1216..2601)
                     /locus_tag="VFO47_09680"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HET8789005.1"
                     /translation="MEVYDSILEVVGGTPLVRLSRLGRGLAPTLLAKVEYLNPGGSVK
                     DRIGLAMVERAERAGQLKPGGTIVEPTSGNTGAGLAIVAALKGYRCVFVMPDKMSQEK
                     IALLRGYGAEVVITPTAVPPDSPESYYSVANRLTAEIPGAVQPNQYANPANPEGHYAS
                     TGPELWDQTGGAIDVFVAGIGTGGTISGAGRYLKERKPDLVVVGADPEGSIYTSDDVH
                     PYLLEGVGEDFWPETFDRSVVDRYVTVPDRDSFLMTRRLAREEGLLVGGSCGLAMVAA
                     VEVAKEYGRDTTIVVLLPDGGRGYLSKVYNDDWMRDHGFLPRRGAATVVDVLGGKAHH
                     DTPPLVTVASHERVKEAIDRLHAYSVSQLPVVHGEDPADLSSVVGSVQEGTLLERLFR
                     KPEVLDAQVVDVMDPPFPTIEQHEQAEAAFELLSKGRSTAVLVTDHGRAVGVMTRSDL
                     LDHLATRTAAR"
     gene            complement(2629..3510)
                     /gene="mca"
                     /locus_tag="VFO47_09685"
     CDS             complement(2629..3510)
                     /gene="mca"
                     /locus_tag="VFO47_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HET8789006.1"
                     /translation="MPRTLLAVHAHPDDESSKGAGTMARYADEGERVVVVTCTGGEAG
                     EILNPAMDRPGVLERMPELRRQELAKALEIIDVTAHHWLGYRDSGMAESETNGHPDAF
                     ANADLDEATGRLVALIRAERPQVVLTYDERGGYPHPDHVRTHEVSVAAFEAAGDPDRF
                     PEAGPPYQPLKLYYHATFSRARMETIHEGAVARGIESPFAEWLERWEEEEESFPEPVI
                     TTQVDVAAWLGRRRDALIAHATQIDPNSFWFAIPDEVVAEVYPWEDYTRVRSLVPVPG
                     HETDLFEGIDPDGNPIR"
BASE COUNT          458 a         1201 c         1399 g          488 t
ORIGIN      
        1 gcccgaccag cgccgccgcc gcggcgacct cggcgacgct gaacgggacc aggtcgtagg
       61 cgccggaggc gacctcgtcg agtagggccc gctcggcctg cacgcccacc cgttcgagca
      121 gcaggtagtc gagctcggcg aggacgaacg gcgacagcag gaaggggccg tcgtcggacg
      181 ccagcacctg gaccgcggac gcatggtgac gctggtcggc gtcgagggcc gcgagcaggc
      241 cgctggtgtc gaggacgatc actcgccgaa gccggccagt gcctcgtcga cccgctcggc
      301 aagggtcggg tcgccgctcc ggaacagcgg cagcgtcggc cggggccgcg cctcctcctc
      361 gagcagccgc tcgatggcgg cgcggatcag cgccgcctcc ggcccgccgc ggcggtgggc
      421 ggcctgggcc agccggcgct tgagcgcgtc ggggaggtag accgtcgtct tctgcatgcg
      481 acgtatggca cctaggctgg cttgacggcc cggaggtgga ggaagttggg gaggcgccgc
      541 cagcgctcgt agccggggcg ggcgtcgagg gcggccggtg gggccggggg ctcgcgcagg
      601 cgctcgatca ggaggccggc ctcctcgagg gcgcgggtgt actcctccag ggagtagcgc
      661 cagccggtga aggtgatctg gaggccgtcg cgctccaggg tgtcctcgaa gcggtggcgg
      721 ccgaggtagg tgccgccgat gacgaacggg gcgtcgggcg ccttggactc gaacttgccg
      781 gcgtcggcga ccgggtgggt gacgcagacg ccgaggcggc cgcccggctc caggacgcgg
      841 gacgcctcgt gcaccgcggc cggcatgtcg tccacgtcca tcagggagtt gtaggcgacc
      901 accaggtcga aggaggcgtc ggcgaagggc agggcggcgg cgtcgccgcg cacgtggtcg
      961 ccgaacgggt tggccgcggc cgcgctggcg atcagggtct cggagatgtc gagggcgacg
     1021 acctggtggc cgcgctggac gaggtcgcgg gtgacccggc cctcgccgca gccgacctcc
     1081 agggtgcggc ggcccgcggc ggggacgatg gcgtcgaaga aggcgtcgcg gaagaaccag
     1141 taggagtcgt ggccgggggt gcgggcccag cggagccagg acccggcctg ccactcccac
     1201 gacatcggcg gcctgctacc gggcggcggt gcgggtggcc aggtggtcga ggaggtcgga
     1261 gcgggtcatg acgccgacgg cgcggccgtg gtcggtgacc aggacggcgg tggagcggcc
     1321 cttggagagg agctcgaagg cggcctcggc ctgctcgtgc tgctcgatgg tggggaacgg
     1381 cgggtccatg acgtcgacga cctgggcgtc gaggacctcg ggcttgcgga acaggcgctc
     1441 caggagggtg ccctcctgga cggagccgac cacgctggag aggtcggccg ggtcctcgcc
     1501 gtggacgacc gggagctggc tgaccgagta ggcgtggagc cggtcgatcg cctccttgac
     1561 ccgctcgtgg gaggcgacgg tgaccagcgg cggggtgtcg tggtgggcct tgccgccgag
     1621 cacgtcgacc acggtggcgg cgccgcggcg gggcaggaac ccgtggtcgc gcatccagtc
     1681 gtcgttgtag accttggaca ggtagccgcg gcccccgtcg ggcagcagga ccacgatggt
     1741 ggtgtcgcgg ccgtactcct tggccacctc gacggcggcg accatggcca gcccgcacga
     1801 cccgccgacc agcagcccct cctcgcgggc caggcggcgg gtcatcagga acgagtcccg
     1861 gtcggggacg gtgacatagc ggtcgaccac cgagcggtcg aaggtctcgg gccagaagtc
     1921 ctcccccacc ccctcgagca ggtaggggtg gacgtcgtcg gaggtgtaga tcgacccctc
     1981 ggggtcggcc ccgaccacca ccaggtcggg cttgcgctcc ttgaggtagc ggccggcccc
     2041 ggagatggtg ccgccggtgc cgatcccggc cacgaacacg tcgatcgccc cgccggtctg
     2101 gtcccacagc tcgggcccgg tgctggcgta gtggccctcg gggttggccg ggttggcgta
     2161 ctggttgggc tggacggcgc cggggatctc ggcggtgagc cggttggcca ccgagtagta
     2221 ggactccggc gagtcgggcg gcaccgccgt cggggtgatc acgacctcgg ccccgtagcc
     2281 ccggagcagg gcgatcttct cctggctcat cttgtccggc atcacgaaca cgcaccggta
     2341 gcccttgagg gcggcgacga tggccagccc ggccccggtg ttgccgctgg tcggctccac
     2401 gatcgtgccg cccggcttga gctggccggc ccgctcggcc cgctcgacca tggccagccc
     2461 gatccggtcc ttgaccgagc cgcccgggtt caggtactcg accttggcca gcagggtcgg
     2521 ggccagtccc cggccaagcc gcgacagccg gaccagcggg gtgcccccga ccacctcgag
     2581 gatcgagtcg tacacctcca tgccgggaag catagcccgt gcggggcgct agcggatggg
     2641 gttgccgtcg gggtcgatcc cctcgaacag gtcggtctcg tgcccgggga ccggcaccag
     2701 ggagcggacc cgggtgtagt cctcccaggg gtacacctcg gccacgacct cgtccgggat
     2761 ggcgaaccag aagctgttgg ggtcgatctg ggtggcgtgg gcgatcaggg cgtcgcgccg
     2821 ccggcccagc caggcggcca cgtcgacctg ggtggtgatg accggctcgg ggaagctctc
     2881 ctcctcttcc tcccagcgct ccagccactc ggcgaacggg ctctcgatcc cgcgggcgac
     2941 cgcgccctcg tggatcgtct ccatccgggc ccggctgaag gtggcgtggt agtagagctt
     3001 gagcggctgg taggggggtc cggcctcggg gaaccgatcg gggtcgccgg cggcctcgaa
     3061 ggcggccacc gagacctcgt gggtgcggac gtggtcgggg tgcgggtagc cgccgcgctc
     3121 gtcgtaggtc agcaccacct ggggccgctc ggcgcggatg agggcgacca gccggccggt
     3181 cgcctcgtcc aggtcggcgt tggcgaaggc gtccgggtgg ccgttggtct cgctctcggc
     3241 catgcccgag tcgcggtagc cgagccagtg gtgggcggtg acgtcgatga tctccagcgc
     3301 cttggccagc tcctggcggc gcagctccgg catccgctcc agcacccccg gccggtccat
     3361 ggccgggttg aggatctcgc cggcctcccc gccggtgcag gtgaccacca cgacccgctc
     3421 gccctcgtcg gcgtagcggg ccatcgtccc ggcgcccttg gacgactcgt cgtcggggtg
     3481 cgcatgcacg gcgagcaggg ttctgggcac gacctcgacg ctcctttcct gggcgggaca
     3541 gccacc
//
LOCUS       DASSWR010000580         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1067121, whole genome shotgun sequence.
ACCESSION   DASSWR010000580 DASSWR010000000
VERSION     DASSWR010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067121"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFO47_09690"
     CDS             complement(<1..271)
                     /locus_tag="VFO47_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8789007.1"
                     /translation="MLRCCAGNRQGKHGAIASRLTDPESGLGLSESQVGLAASIYVLG
                     ACLGALFFGYLTDRFGRKKLFIITLVVYLVATLLTALSFSAWWFFL"
     gene            297..1583
                     /locus_tag="VFO47_09695"
     CDS             297..1583
                     /locus_tag="VFO47_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8789008.1"
                     /translation="MTGTGPVRVAIACQGGGSHTAFTAGVLKRLLGAEELAGIEVVGL
                     SGTSGGAVCALLAWSALLDDDPAAAGKLLEAFWADNSATTPLKQLVNAWVLWAARLEH
                     LVVLPAVSPYDTPTSVAALEEFRAMLERRVDFGRFAVPAGESRPMLLIGAVDVLSGEF
                     RAFNSRRDPITADTILASAAIPNLFRSVRVGDGTYWDGLFSQNPPVRELVDTRPDELW
                     VIQINPRRWDGEPRTMVEIADRRNELAGNLSLHQELGFIEKIDQLLAEGRLAADGRYK
                     QITVRVLELSRSRLSRSLGTASKLNRDPGFIRDLIAHGEAMAEEFLAALAFERAWRRR
                     DPGAVLACFADDAELVSAPPFPDRGSLRGRQRIGRFVREHLTADLHVDPTRKQVARDR
                     VTWTVQAYRDDPAAAVRGRAEAELRTGRITALRLGG"
     gene            complement(1584..2312)
                     /locus_tag="VFO47_09700"
     CDS             complement(1584..2312)
                     /locus_tag="VFO47_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8789009.1"
                     /translation="MIRVAAVGDVHVGVDSAGRLAPRLAGLADHADLFLLAGDLTHRG
                     RPEEAKVLAEELRGVGVPTVAVLGNHDYHSDEQDAVTEVLEEAGIRVLEGEAAVLEVG
                     GRRLGIAGSKGFGGGFAGASASDFGEPEMKAFVGHTKTLAGRLERALGGLRADRRIAL
                     LHYSPVAETLAGEPREIHAFLGSYLLAEAVDRAGADLVLHGHAHRGSRTGTTPGGVPV
                     RNVAAPVIGRAYEVFRFDAEDAPA"
     gene            complement(2309..>2694)
                     /locus_tag="VFO47_09705"
     CDS             complement(2309..>2694)
                     /locus_tag="VFO47_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET8789010.1"
                     /translation="LLRRAAPGPRRVASLLLYAQTDGLRVPDWVIESLLEQAREQETG
                     ADVPDEYLVAHANEALAGDPQTAELELDVAIDGREVVVTGTVATPERQAAVAEVIAKA
                     LPGREVRNLTDVEDAAADPFVETLP"
BASE COUNT          344 a         1017 c          984 g          349 t
ORIGIN      
        1 acaggaagaa ccaccacgcg ctgaaggaga gcgcggtcag cagcgtcgcc accagataga
       61 cgaccagcgt gatgatgaac agcttcttgc ggccgaaccg gtcggtcagg tagccgaaga
      121 acaacgcccc caggcaggcc ccgagcacgt agatggaggc cgccaggccg acctggctct
      181 cgctcagccc gaggccgctc tcggggtcgg tcagccggct ggcgatggcc ccatgcttgc
      241 cctgcctgtt gccagcgcag cagcggagca aacaagcggt cccgtagcat ggacggatga
      301 ccggaacggg acctgtccgg gtggcgatcg cctgccaggg cggcggcagc cacaccgcct
      361 tcacggccgg ggtgctcaag cgccttctcg gcgccgagga gctggccggg atcgaggtcg
      421 tcggcctcag cggcacctcc ggcggggcgg tctgcgccct gctcgcctgg tcggccctgc
      481 tcgacgacga cccggccgcc gccggcaagc tgctggaggc gttctgggcc gacaactcgg
      541 ccacgacccc gctgaagcag ctggtcaacg cctgggtgct gtgggcggcc aggctggagc
      601 acctggtcgt cctgccggcg gtcagcccgt acgacacgcc gacgtcggtg gccgcccttg
      661 aggagttccg ggcgatgctg gagcggcggg tcgacttcgg gcgcttcgcc gtcccggccg
      721 gcgagtcgcg tcccatgctg ctgatcgggg ccgtggacgt cctctcgggc gagttccggg
      781 cgttcaacag ccgtcgcgac ccgatcaccg ccgacacgat cctggcctcg gcggccatcc
      841 ccaacctgtt ccgctccgtc cgggtcggcg acggcaccta ctgggacggc ctgttctccc
      901 agaacccgcc cgtccgggag ctggtcgaca cccgccccga cgagctctgg gtgatccaga
      961 tcaaccccag gcgctgggac ggggagccgc ggacgatggt cgagatcgcc gaccgccgca
     1021 acgagctggc cggcaacctc tcgctgcacc aggagctcgg cttcatcgag aagatcgacc
     1081 agctgctggc cgagggccgg ctggccgccg acggccgcta caagcagatc acggtgcggg
     1141 tgctggagct gtcccggtcg cggctgtccc ggtcgctcgg caccgcctcc aagctcaacc
     1201 gcgaccccgg cttcatccgc gacctgatcg cccacggcga ggccatggcc gaggagttcc
     1261 tggccgcgct ggccttcgag cgcgcctggc ggcgccgtga ccccggggcg gtgctggcct
     1321 gcttcgccga cgacgccgag ctggtctcgg cgccgccctt cccggaccgg ggcagcctcc
     1381 gcggccgcca gcggatcggc cgcttcgtgc gcgagcacct gactgccgac ctgcacgtcg
     1441 acccgacccg caagcaggtg gcccgcgacc gggtgacctg gaccgtgcag gcctaccgcg
     1501 acgacccggc ggcggcggtc cggggccggg ccgaggccga gcttcggacg ggccggatca
     1561 ccgccctgcg cctgggcggc tgatcaggcc ggggcgtcct cggcgtcgaa gcggaacacc
     1621 tcgtaggcgc ggccgatcac gggggcggcg acgttgcgca ccggcacccc gccgggggtg
     1681 gtgccggtgc ggctgccccg gtgggcgtgg ccgtgcagga ccaggtcggc cccggcccgg
     1741 tcgacggcct cggccagcag gtagctgccg aggaaggcgt ggatctcgcg gggctcgccg
     1801 gccagggtct cggccaccgg cgagtagtgc agcagggcga tccggcggtc cgcccggagc
     1861 cccccgaggg cccgttccag ccggccggcc agggtcttgg tgtggccgac gaacgccttc
     1921 atctccggtt cgccgaagtc gctggcgctg gccccggcga agccgccgcc gaaccccttg
     1981 ctgccggcga tcccgagccg gcggccgccc acctccagca ccgccgcctc accctccagg
     2041 acgcggatgc cggcctcctc gaggacctcg gtgacggcgt cctgctcgtc ggagtggtag
     2101 tcgtggttgc cgaggacggc caccgtcggc accccgacgc cgcgcagctc ctcggccagc
     2161 accttggcct cctcgggccg gccccggtgg gtgaggtcgc cggccagcag gaacaggtcg
     2221 gcgtggtcgg ccaggccggc caggcgcggg gccaggcggc ccgccgagtc gaccccgacg
     2281 tggacgtcgc cgacggccgc cacccggatc acggcagcgt ctccacgaac gggtcggccg
     2341 ccgcgtcctc gacgtcggtc aggttgcgga cctcccggcc gggcagggcc ttggcgatga
     2401 cctcggccac ggcggcctgc cgctcgggcg tggccaccgt cccggtcacc accacctcgc
     2461 gcccgtcgat ggcgacgtcc agctccagct cggccgtctg ggggtcgccg gccagggcct
     2521 cgttggcgtg ggcgaccagg tactcgtcgg gcacgtcggc gccggtctcc tgctcgcggg
     2581 cctgctccag cagcgactcg atcacccagt cgggcacccg cagcccgtcg gtctgggcgt
     2641 acagcagcag gctggccacc cggcgcgggc ccggggcggc ccggcgcagc aggt
//
LOCUS       DASSWR010000581         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1237241, whole genome shotgun sequence.
ACCESSION   DASSWR010000581 DASSWR010000000
VERSION     DASSWR010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237241"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1125
                     /locus_tag="VFO47_09710"
     CDS             355..1125
                     /locus_tag="VFO47_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8789011.1"
                     /translation="MAVTAEVEDRVGWITLDRPPDNRFDAAFLEELDRCVGEVAEAEV
                     VVAVIRSASDKFFSAGADVAGLLERSPDENLARCRRAHQVLGRFADESPLFVAAIAGH
                     ALGNGYELALACDIRIAAEGRYRIGLPDVALGLLPSSGGAHRLSRLVGLGRALDLVAT
                     GRTVAPAEAERIGMVDRLVPAAEFDTAVAELAAGLAAGAPLAVAAITRVVNDGIERSL
                     ADALDRDLEELADLLASEDAREGMTAFTEQRPPSFNGR"
     gene            complement(1164..2063)
                     /locus_tag="VFO47_09715"
     CDS             complement(1164..2063)
                     /locus_tag="VFO47_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8789012.1"
                     /translation="MPTSRRAVMKKSTIVRSFALLERIAPAVGARWAESLWFTVPNGR
                     GGHDRLAPPGRPFQVEVHGRTVAGEVWGERPAVGAPDPAGPPVVYLVHGWGGWRWQLD
                     AFAAPLVEAGFRVVAFDAPSHGASDPGPEGPGRSSLLEFADALAAVVAANGPAHAVIA
                     HSLGATAAAYAVRAGLPVGRLAFVAPMADPLPYTRTFAGRLGFGEQVRTRMVARVERR
                     VGTSMSAFDVPAMARQVATPPLLLVHDRQDAETGWSDSAAIAQSWPDTRLITTTGLGH
                     RRILRAPAVVAEVTGFVGDRVRT"
     gene            complement(2074..2667)
                     /locus_tag="VFO47_09720"
     CDS             complement(2074..2667)
                     /locus_tag="VFO47_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8789013.1"
                     /translation="MSKGTATRQAVLDEAARVASRVGLSGLTIGSLAASTQLSKSGLF
                     AHFQSKESLQLQLLDHTRVRFIDMVIRPALAAPRGEPRVRELFRRWLAWDAEALPGGC
                     LFVAASAEFDGQPGPVRDRLAQDQRDWLDTIAQVFRTGVAEGHFRPDADPVQFAYDLH
                     GTMLAYHHASRLLDDPDAAARATRAFEALVAAARPAS"
     gene            complement(2901..3413)
                     /locus_tag="VFO47_09725"
                     /pseudo
     CDS             complement(2901..3413)
                     /locus_tag="VFO47_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          430 a         1192 c         1382 g          409 t
ORIGIN      
        1 agtagggccg gggtcgggcc cattcagtca tcggacgggg cgaacctcac ccctgacccg
       61 ggcgagcctc acccaccccg gccggcgtgg ctcctcggag gccgggcccc attcacgcac
      121 ctggcgcggg gcgcgcccca ccccgccggg tcggcgctcg gccccccgga gggggagggt
      181 agccgatcgc cgggagcggg ggatctggtc gtccacagcc cccaggcgcc gcactccatc
      241 aatggccgat tcagggatcg gggcgccgcc ctcgaccaac cggtcaggca ggtgggacga
      301 taccgtccgc cgatttctgg cgacaggccc gtacgcgacc aggggggaca gccgatggcg
      361 gtgaccgccg aggtcgaaga ccgggtcggc tggatcaccc tggaccgccc gccggacaac
      421 cgcttcgacg cggccttcct ggaggagctg gaccgctgcg tcggcgaggt cgccgaggcc
      481 gaggtggtgg tggccgtgat ccggtcggcc agcgacaagt tcttctcggc cggggccgac
      541 gtggccgggc tcctggagcg gtcgcccgac gagaacctgg ccaggtgccg ccgcgcccac
      601 caggtgctcg gccggttcgc ggacgagtcg ccgctgttcg tcgccgccat cgccgggcac
      661 gccctcggca atggctacga gctcgcgctc gcctgcgaca tccgcatcgc cgccgagggg
      721 cgctaccgga tcgggctgcc cgacgtcgcc cttggcctgc tgccctcctc cggcggcgcc
      781 catcggctgt cccgcctggt cggcctcggc cgggccctgg acctggtggc caccggccgg
      841 accgtggccc cggccgaggc cgagcgcatc ggcatggtcg accggctggt cccggcggcc
      901 gagttcgaca cggccgtggc cgagctggcc gccggcctgg ccgccggcgc ccccctggcc
      961 gtggcggcca tcacccgggt ggtcaacgac gggatcgagc gctccctggc cgacgccctc
     1021 gaccgggacc tggaggagct ggccgacctg ctcgcctccg aggacgcccg cgagggcatg
     1081 accgccttca ccgagcagcg cccgcccagc ttcaacggcc gctgacgcac cctgctcggc
     1141 gccggccagg ccggccgccg cggtcaggta cggacgcggt cgcccacgaa cccggtgacc
     1201 tcggcgacca cggcgggggc gcggaggatg cggcggtgac cgaggccggt ggtggtgatc
     1261 aggcgggtgt ccggccagga ctgggcgatg gcggcgctgt ccgaccagcc ggtctcggcg
     1321 tcctggcggt cgtggacgag cagcagcggc ggggtggcga cctggcgggc catggcgggc
     1381 acgtcaaagg ccgacatgga ggtgccgacc cggcgctcca ccctggccac catgcgggtc
     1441 cggacctgct cgccgaaccc gaggcggccg gcgaaggtcc gggtgtaggg cagggggtcg
     1501 gccatggggg cgacgaaggc caggcggccg acgggcaggc cggcgcgcac cgcgtaggcg
     1561 gcggccgtgg ctccgaggga gtgggcgatc accgcgtggg ccgggccgtt ggcggccacc
     1621 accgcggcca gggcgtcggc gaactcgagg agggagctgc gcccggggcc ttcggggccg
     1681 gggtcggagg cgccgtggct gggggcgtcg aaggcgacca cgcggaagcc ggcctcgacc
     1741 agcggggcgg cgaaggcgtc gagctgccag cgccacccgc cccagccgtg gaccaggtag
     1801 acgaccgggg ggccggccgg gtcgggcgcg cccacggccg gtcgttcgcc ccagacctcg
     1861 ccggcgaccg tgcggccgtg gacctcgacc tggaaggggc ggcccggggg cgccagccgg
     1921 tcgtggccgc cgcgcccgtt ggggacggtg aaccagagcg actccgccca gcgggccccg
     1981 acggcgggag cgatccgctc gagcagggcg aatgaccgaa cgatcgtgct tttcttcatc
     2041 actgcgcgac ggctggtcgg catggaggtc tcctcaggag gcggggcggg cggccgccac
     2101 cagcgcctcg aaggcgcggg tggcgcgggc ggcggcgtcg gggtcgtcga gcagccggct
     2161 ggcgtggtgg taggccagca tcgtcccgtg caggtcgtag gcgaactgga cggggtcggc
     2221 gtcggggcgg aagtggccct cggcgacgcc ggtgcggaac acctgggcga tcgtgtccag
     2281 ccagtcgcgc tggtcctggg ccagccggtc gcggacgggg ccgggctggc cgtcgaactc
     2341 ggcgctggcg gcgacgaaca ggcagccgcc gggcagggcc tcggcgtccc aggcgagcca
     2401 gcgccggaac agctcgcgca cccggggctc gccgcggggc gcggccaggg cggggcggat
     2461 caccatgtcg atgaagcgga cccgggtgtg gtcgagcagc tggagctgga gcgactcctt
     2521 ggactggaag tgggcgaaca ggccgctctt ggagagctgg gtcgaggcgg ccagcgaccc
     2581 gatggtcagc ccgctcagcc cgacccggct ggccaccctg gccgcctcgt cgagtacggc
     2641 ctgcctggtc gccgttccct tgctcatggg ttcactaaaa gcacgaacgt tcgtctacat
     2701 caagaggggg agggcgagtc agcttcggcg gggggctccg aggggaccgg gggcgcatcg
     2761 gcgtcgggcg gatccgcggc gggcgcatcg gcgtcgggcg catcgacgtc gggcgcatcc
     2821 gcggcagggg cggccgcggc cgggcgggag cgggtggccc aggtgctcgg gtcgtccctg
     2881 aggccttcga ggacccagca ggcgatgggg tcgcgcttgc ccttgacctc gatggggtcg
     2941 agggggcgga cgatggcgag gtgcttgatg gcggcctggg tggtggggcc gaccacgacc
     3001 tggccggcct cggcgacctc ctgctcgagg cgggcggcca ggttgacggt gtcgccgatg
     3061 gcggtgaagt tgcggaactc gtcgctgccg atgttgccga tcagggcggg cccggtgttg
     3121 accccgatcc ggaaccgggg ccagtcgggc tcgcgctcgg cgacggcctc gatggccgcc
     3181 tgcatgccaa gggccgcccg ggccgcccgc agggcgtggt cgggctggcg ggccggggcg
     3241 ttgaacagga ccatgacggc gtcgccgacg aactggacca ccgtgccccc gttggccagc
     3301 agcaccggca cggcccggcc gaagtactgg ttgagcatgg ccacgacccg ctccggggag
     3361 gtccgctcgg agaacggcgt gaacccgcgc aggtcggcga acaggaccgt cac
//
LOCUS       DASSWR010000582         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_333048,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000582 DASSWR010000000
VERSION     DASSWR010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333048"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..2034)
                     /locus_tag="VFO47_09730"
     CDS             complement(184..2034)
                     /locus_tag="VFO47_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8789014.1"
                     /translation="MSEDRVEIERREIEQEIRGQTLCDLLADHAAGHGDAPAMSWKRD
                     GAWQTLSWRDYRERVAEVAMGLAGLGVGKGDFVAIMATNRPEHVLADQAAIHAGATPS
                     SFYFTLAPEQIQYVAGHCEAKVAVLEDRDMLKRWQELRDNLPALRYVVLLDGAADDDG
                     PGVLGWDELVRRGREALAADRSGFEELRGRVGPEDNATLLYTSGTTGPPKGVLLTHRN
                     LLFECTALRRLTELDFEGSSVSYLPLAHIAERVLSIYVPLTLRTHVWFCPEPTQAVEY
                     VREARPTLFFGVPRVWEKVRAGIDAKLAAEENERKRKIAQAALAAGRQVVRRQQKGEP
                     VPFGLRARHAVLERLVLAKVRHAIGLDRCIFASSAASPLAVDVAEYFAALGLPLVEVY
                     GMTETTGGATGNRPGTVKIGTVGPALAGVEVRLADDGEVLVRGPVNTPGYFRQPEATA
                     ELIDAEGWLHTGDVGELDGDGYLKIVDRKKELIITSSGKNLSPANIEGLLKEHPLVGQ
                     ALVYGDDRPYVVALIVLDHELAPGWAARNGLGTADLATLAGHEQVLTEIQLAVDGANQ
                     HLARIEQVKRFEVLPVEWTAESEELTPTLKLRRRIIHAKYAERIDALYQG"
BASE COUNT          286 a          821 c          876 g          329 t
ORIGIN      
        1 gacccgggag gagccggtcc ggccggaggc ggcgtgggcg gtgctctggg ccgacctggg
       61 cgagagcgtg cggcgacccg ggaacgggac cggggcgggc ggcgacggcg acgacctgat
      121 ggcggtggcc aacgccgtcc aggcccgccg ccgggtcagg acgctgcgcc gctgagcgcg
      181 gggtcagccc tggtacaggg cgtcgatgcg ctcggcgtac ttggcgtgga tgatgcggcg
      241 gcggagcttg agggtggggg tgagctcctc gctctcggcg gtccactcga ccgggaggac
      301 ctcgaaccgc ttgacctgct cgatccgggc caggtgctgg ttggcgccgt cgacggccag
      361 ctggatctcg gtgagcacct gctcgtggcc ggcgagggtg gccaggtcgg cggtgccgag
      421 gccgttgcgg gcggcccacc cgggggcgag ctcatggtcg agcacgatca gtgcgaccac
      481 gtacggccgg tcgtcgccgt agaccagcgc ctggccgacc agcgggtgct ccttgagcag
      541 gccctcgatg ttggccgggg acaggttctt gccgctggag gtgatgatca gctctttctt
      601 gcggtcgacg atcttgaggt agccgtcgcc gtccagttcc ccgacgtcgc cggtgtgcag
      661 ccagccctcg gcgtcgatca gctcggccgt cgcctccggc tgccggaagt acccgggggt
      721 gttgaccggc ccccggacca gcacctcgcc gtcgtcggcc agccgcacct cgaccccggc
      781 cagggccggg ccgacggtgc cgatcttgac cgtgccgggc cggttgccgg tggccccccc
      841 cgtggtctcg gtcatgccgt acacctcgac caggggcagg ccgagggccg cgaagtactc
      901 ggccacgtcc acggccaggg gcgaggccgc gctggaggcg aagatgcagc ggtccagccc
      961 gatggcgtgg cggaccttgg ccagcaccag ccgctcgagc acggcgtgcc gggcccgcag
     1021 cccgaagggc accggctcgc ccttctgctg gcggcgcacg acctggcgcc ccgcggccag
     1081 ggccgcctgg gcgatcttgc gcttgcgctc gttctcctcg gcggccagct tggcgtcgat
     1141 gccggcccgg accttctccc acaccctggg cacgccgaag aacaacgtcg gccgggcctc
     1201 gcggacgtac tcgaccgcct gggtcggctc ggggcagaac cagacgtggg tccgcagggt
     1261 cagcggcacg tagatcgaca gcacccgctc ggcgatgtgg gccaggggca ggtaggagac
     1321 cgagctgccc tcgaagtcga gctcggtcag gcgccgcagc gccgtgcact cgaagagcag
     1381 gttgcggtgg gtcaggagca cacccttggg cgggccggtg gtgccggagg tgtagagcag
     1441 ggtggcgttg tcctcggggc cgacccggcc gcgcagctcc tcgaagccgg agcggtcggc
     1501 ggccagcgcc tcgcggcccc ggcggaccag ctcgtcccag ccgagcacgc ccggcccgtc
     1561 gtcgtcggcg gcgccgtcca gcaggaccac gtagcgcagg gcgggcaggt tgtcgcggag
     1621 ctcctgccag cgcttgagca tgtcccggtc ctcgagcacg gccaccttgg cctcgcagtg
     1681 cccggccacg tactggatct gctcgggggc gagggtgaag tagaagctcg acggggtggc
     1741 cccggcgtgg atggcggcct ggtcggcgag gacgtgctcg ggccggttgg tggccatgat
     1801 ggccacgaag tcgcccttgc cgacccccag gccggccagg cccatggcca cctcggcgac
     1861 ccgctcgcgg tagtcgcgcc acgacagcgt ctgccaggcg ccgtcgcgct tccaggacat
     1921 ggccggcgcg tcgccgtgac cggccgcgtg gtcggcgagc aggtcgcaca gggtctggcc
     1981 cctgatctcc tgctcgatct cccggcgctc gatctcgacc cggtcttcgc tcatcgcgac
     2041 ccaccctcgg ctcggagagg ctgcacgctg gcgagcatag gtagcgggac cgggcaggta
     2101 aagggcctgg cccccgtgag cgactcgttg gcgggccccg gcaggcttgt cctgtccagc
     2161 gccgagcgcg aaaggatggt ggcgatgtcc cggttccccc aggccggcct gcgctacctg
     2221 gccgccctgg actacgcccg ccgcggcctg cccgtcgtcc ccctgcacta cgtggtgcgc
     2281 acccggcgga ccaccgccgt cacctgctcg tg
//
LOCUS       DASSWR010000583         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_473136,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000583 DASSWR010000000
VERSION     DASSWR010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473136"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VFO47_09735"
     CDS             <1..1978
                     /locus_tag="VFO47_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8789015.1"
                     /translation="PGREVVLDVLKKEELTDVDGSDSQHPNRSGDVVVIARPPYQFDA
                     ATPGVAIAPSEFFGQHGYRPDMVNIARNLNMHGTFVAAGPGIRNRGPVAGIRAIDVAP
                     TLAFLMGIPGPQTAQGRILFEILKGGSRYFDAHLLGINDLHGNLTGAGQIYTDPYSGF
                     RGAAGGAAVLARYLLERKRANPGTTVLVHSGDMVGASPPESGLLQDEPTIRVLNEIGF
                     DVGAPGNHEFDEGLEELLRLLNGGPSQFPPGSTFEGQDFPLVSANIVDADTKEPIFDP
                     YLIKRIKGVPMAFIGATTITTPTVVEQGAVDGLEFLDEAEAVNSYVPELKAKGVEAMV
                     LLIHEGGTQDRFPFGTISPRISDVTRALDPEVDVVMAGHSHTALNSRVDGRLVVQASS
                     FGRAFEDVRITLDRKTKDVAAASATLQGVWTYNPPDIADPAHLVEGDPGVQAIVDDAV
                     EQVAPLVNRVVNVAATDLLAGRDGGANAAGESPLGNLIADAQRAEMGTQFAFMNPGGI
                     RARIQAGEVTWGELFTVQPFANDLVKMDLTGAQIWTLLGQQFQTPSNRILEISGLHYR
                     YHLTSPTTGVIDAVFVGPPGDDSTPVPNDASVTYTVTVNSFLAGGGDGFTVLREGTNR
                     VVGPVDLDALVEYVRGLRPPPFDSQIEGRIQLTQ"
     gene            complement(2016..>2335)
                     /locus_tag="VFO47_09740"
     CDS             complement(2016..>2335)
                     /locus_tag="VFO47_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8789016.1"
                     /translation="TDGESRSGTPPLTAAQQAREEGVPVFTVAIAPQPPQELQEIAEV
                     SGGQAFSAPTSAELEAVYRDLGTRLTYVEEKRELTSYFMGGAALFLLLGAAASITWFL
                     RLP"
BASE COUNT          358 a          871 c          802 g          304 t
ORIGIN      
        1 acccggccgc gaggtcgtcc tcgacgtgct caagaaggag gagctgaccg acgtcgacgg
       61 cagcgactcc cagcacccga accgctcggg cgacgtggtc gtgatcgccc ggccgcccta
      121 ccagttcgac gcggccaccc cgggcgtggc catcgcgccc tcggagttct tcggccagca
      181 cggctaccgg ccggacatgg tgaacatcgc ccgcaacctc aacatgcacg gcaccttcgt
      241 ggccgccggg cccgggatcc gcaaccgcgg gccggtggcc ggcatccggg cgatcgacgt
      301 cgcgccgacg ctcgccttcc tcatgggcat ccccggcccg cagacggcac agggccggat
      361 cctgttcgag atcctcaagg gcggcagccg ctacttcgac gcccacctgc tggggatcaa
      421 cgacctgcac ggcaacctca ccggggccgg gcagatctac accgacccct acagcgggtt
      481 ccgcggggcg gccgggggcg cggccgtgct cgcccgctac ctgctggagc gcaagcgggc
      541 caaccccggc accaccgtgc tggtccactc cggcgacatg gtcggggcca gcccgcccga
      601 gtcggggctg ctccaggacg agccgaccat ccgcgtcctc aacgagatcg gcttcgacgt
      661 cggcgccccc ggcaaccacg agttcgacga gggcctggag gagctgctgc ggctgctcaa
      721 cggcggcccg tcccagttcc cgccgggcag cacgttcgag ggccaggact tcccgctggt
      781 gtcggccaac atcgtcgacg ccgacaccaa ggagccgatc ttcgacccct acctgatcaa
      841 gcggatcaag ggcgtcccga tggccttcat cggcgccacc accatcacca ccccgaccgt
      901 cgtcgagcag ggggcggtcg acggcctcga gttcctggac gaggccgagg ccgtcaacag
      961 ctacgtgccc gagctcaagg ccaagggcgt cgaggcgatg gtgctgctga tccacgaggg
     1021 cggcacccag gaccggttcc ccttcgggac catctcgccc cggatcagcg acgtcacccg
     1081 ggccctcgac cccgaggtcg acgtggtcat ggccgggcac tcccacaccg ccctgaactc
     1141 gcgggtcgac gggcgcctgg tcgtgcaggc gtcgtcgttc gggcgcgcgt tcgaggacgt
     1201 ccgcatcacc ctcgaccgca agaccaagga cgtcgcggcg gcctcggcga ccctgcaggg
     1261 ggtctggacc tacaacccgc ccgacatcgc cgacccggcc cacctggtcg aaggggaccc
     1321 gggcgtccag gcgatcgtcg acgacgccgt cgagcaggtc gcgccgctgg tcaaccgggt
     1381 cgtcaacgtc gccgccaccg acctgctggc cgggcgggac ggcggggcca acgcggccgg
     1441 cgagtcgccg ctcggcaacc tgatcgccga cgcccagcgg gccgagatgg gcacccagtt
     1501 cgccttcatg aacccgggcg gcatccgggc ccggatccag gccggcgagg tcacctgggg
     1561 ggagctgttc acggtccagc cgttcgccaa cgacctggtc aagatggacc tgaccggggc
     1621 ccagatctgg accctgctgg gccagcagtt ccagacgccc tcgaaccgga tcctggagat
     1681 ctcgggcctc cactaccgct accacctgac ctccccgacc accggggtga tcgacgccgt
     1741 cttcgtcggc cccccgggtg acgactccac cccggtgccc aacgacgcca gcgtcaccta
     1801 cacggtcacg gtcaactcgt tcctggccgg cggcggcgac gggttcacgg tgctccgcga
     1861 gggcaccaac cgcgtggtcg gcccggtcga cctcgacgcc ctggtcgagt acgtcagggg
     1921 cctcagacca cccccgttcg acagccagat cgaggggagg atccagctca cccagtaggc
     1981 cggccagctc cgcggaccac cctgatccgg ccgggtcagg gcaggcggag gaaccaggtg
     2041 atcgaggcgg cggcgccgag gaggaggaac agggccgccc cgcccatgaa gtacgaggtg
     2101 agctcgcgct tctcctcgac gtaggtgagg cgggtgccga ggtcgcggta gacggcctcg
     2161 agctcggcgc tggtcggggc ggagaaggct tggccgccgc tgacctcggc gatctcctgg
     2221 agctcctggg gcggctgggg ggcaatggcg accgtgaaga ccggcacccc ctcctcgcgg
     2281 gcctgctggg cggcggtcag gggcggggtg ccgctgcggc tctcaccgtc ggtga
//
LOCUS       DASSWR010000584         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_304353,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000584 DASSWR010000000
VERSION     DASSWR010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304353"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VFO47_09745"
     CDS             <1..151
                     /locus_tag="VFO47_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HET8789017.1"
                     /translation="AHRRARTEASGGITLERARAVAETGVDFLAVGALTHSATALDIA
                     LDWEG"
     gene            155..940
                     /locus_tag="VFO47_09750"
     CDS             155..940
                     /locus_tag="VFO47_09750"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016645308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET8789018.1"
                     /translation="MLLAVDVGNTEITIGVFQGEELAQHWRASTVAERTADEHALLLD
                     GFLAQEGLGLGDASGVVVSSVVPRLTQALREMVRRYCQVEPLVIEPGVRTGLPILTDN
                     PREVGADRVVNAIAAYAAYGGPVVVVDFGTATTFDAVSERGEHLGAAIAPGIQISMNA
                     LVERTAQLRRVELVEPRSVIGKNTVESLQAGAIWGFAGQVDGIVRRMVAELGGEATVV
                     ATGGLAGAVLEACETVQRHEPWLTLHGLRIIWDRNRGSGGLKQ"
     gene            937..1563
                     /locus_tag="VFO47_09755"
     CDS             937..1563
                     /locus_tag="VFO47_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789019.1"
                     /translation="MSDRLPRQDRVEGRALSPRAFRPFGIEAVVLGPPTTQEGLVRRE
                     GVAVWRRMAEHAPVFDRMAATATVDRDGLLPVRVVVAATPARDRAMRVPALGERAARV
                     QAATDPPPAAGWLLLVDEGPGETWLYLAEEDHPAAVLDGFLGRRGRTDFARARRRSGV
                     GSLASLGLLAGLFLAIYGSALGLGPPGQFAGAGIIVASLAALIAARPR"
     gene            2104..>3468
                     /locus_tag="VFO47_09760"
     CDS             2104..>3468
                     /locus_tag="VFO47_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET8789020.1"
                     /translation="MTDRIDPEPDDRPPPGAPGHVTSIEDPEETRLNRVITRPMLTFF
                     ILGDILGAGIYALTGQVAGDVGGAIWASFLVSFLLALVTAFAYLELVTKYPRAAGAAL
                     YVHRAFHISILSFMVTIAVMASGVTSASFAATRVGGRYWTGVFGEENPPLKLIAVLVI
                     LLVAAVNYRGVGESIKVNIAITLIELSGLLFIILVGVKVLFSGDGDPGQAFEFKDSGF
                     GALTGITAGAATAFYAFIGFEDSVNMAEEVRDPINTFPPALLTGIVAASIIYLLVGFT
                     AAMTVPLDVLTESTGPLLEVVRIGLPDYDAGTLFSVIAMIAVSNTMLINMLMASRLLY
                     GMANQGVLPAPFAWVSPRKTPWFGILFTTALSILLILTVNDLSGLSDTTVLLLTSVFL
                     LVNICVLVLRRDPVGHSHFVAPTIMPIIGALVALVFLLPINRTGEVYRTAIYLLIGGL
                     VLWGI"
BASE COUNT          440 a         1271 c         1223 g          534 t
ORIGIN      
        1 cgcccaccgg cgggcccgca ccgaggcgtc gggcggcatc accctggaac gggcccgggc
       61 ggtcgccgag accggggtcg acttcctggc tgtcggcgcc ctcacccact cggccacggc
      121 cctggacatc gccctcgact gggagggatg accggtgctg ctcgctgtcg acgtcggcaa
      181 caccgagatc accatcggcg tgttccaggg cgaggagctg gcccagcact ggcgggcgtc
      241 gaccgtggcc gagcgcactg ccgacgagca cgccctgctg ctggacggct tcctggccca
      301 ggaggggctg gggctgggcg acgcctccgg ggtggtggtc tcctcggtgg tgccgcggct
      361 cacccaggcc ctgcgcgaga tggtccggcg ctactgccag gtcgagccgc tggtgatcga
      421 gcccggcgtc cgcaccgggc tgccgatcct gaccgacaac ccgcgcgagg tcggggccga
      481 ccgggtggtg aacgccatcg ccgcctacgc cgcctacggc gggccggtgg tggtggtcga
      541 cttcggtacc gccaccacct tcgacgccgt ctccgagcgg ggcgagcacc tgggcgcggc
      601 catcgccccc ggcatccaga tctccatgaa cgccctggtc gagcggaccg cccagctgcg
      661 ccgggtcgag ctggtggagc cccggtcggt gatcggcaag aacaccgtgg agagcctcca
      721 ggcgggggcc atctggggct tcgccggcca ggtcgacggc atcgtccggc gcatggtggc
      781 cgagctgggc ggcgaggcca ccgtggtcgc caccggcggc ctggccgggg ccgtgctgga
      841 ggcgtgcgag accgtccagc gccacgagcc ctggctcacc ctccacggtc tgcggatcat
      901 ctgggacagg aacagggggt ccggggggct caagcaatga gtgaccggct gccgcgccag
      961 gaccgggtcg agggccgggc cctgtcgccg cgggcgttcc ggccgttcgg gatcgaggcg
     1021 gtcgtgctcg gcccgccgac cacccaggag gggctggtcc gccgcgaggg cgtggccgtg
     1081 tggcggcgca tggccgagca cgcgccggtg ttcgaccgga tggccgcaac ggccaccgtc
     1141 gaccgcgacg ggctgctgcc ggtgcgggtg gtggtggccg ccaccccggc cagggaccgg
     1201 gccatgcgcg tcccggccct cggcgagcgc gcggccaggg tccaggccgc caccgacccg
     1261 ccgccggcgg ccggctggct cctgctggtc gacgagggcc cgggcgagac ctggctctac
     1321 ctggccgagg aggaccaccc ggccgccgtc ctcgacggct tcctcggccg ccgcggccgc
     1381 accgacttcg cccgggcccg ccgccgctcc ggcgtcggca gcctggccag cctcggcctg
     1441 ctggccgggt tgttcctggc catctacggc agcgccctcg gcctcggccc ccccggccag
     1501 ttcgccggcg ccgggatcat cgtcgcctcc ctggccgcgc tgatcgcggc aaggccccgc
     1561 tgagaagtcg ggcacgcagc tgcgcagcac ggacggccga gatcttgggg gggtcggagg
     1621 gggaggactc cccccgaatg aaccgaggac tacaggcagc ggtccctatg cgaggcttcg
     1681 acgttgcccg tgcgcggctt gcgccggtcc aaccccggcc cctgggggat gggggggcgg
     1741 gggccggggt tggactggga ggtcgctcca cacgccctgg cgggtaccgg ggcggccggt
     1801 ccgggtagcg acctcgacga cccgacgccg aggcggaggt cagatgccct cggcgcggcg
     1861 gcgacccagc tccgggtctg cctgccaggg aacgcggtcg tcgtcatcgg cgcggctccg
     1921 gcgggggtag gtatcccgat cgcgagggtc cgcctggagc ctggacaaac gatgtcgtga
     1981 cgacctaacc gggcggtaac gacagcacgg gcgaatcgcg cctctaggtc gcgtcctgcg
     2041 ttcttgttat ggttctactg cgtgttccca tccggtgggt agcctcccca ggaggtggtc
     2101 agcttgaccg acaggatcga ccccgagccg gacgaccgtc cgcctcccgg agcacccggc
     2161 cacgtcacca gcatcgagga ccccgaggag accaggctca accgggtgat cacccggccg
     2221 atgctgacct tcttcatcct cggcgacatc ctcggggccg gcatctacgc cctcaccggg
     2281 caggtcgcgg gcgacgtcgg cggggccatc tgggcctcgt tcctggtctc gttcctgctc
     2341 gccctggtca ccgccttcgc ctacctggag ctggtgacca agtacccgag ggccgccggc
     2401 gccgccctgt acgtccaccg ggcgttccac atctcgatcc ttagcttcat ggtgaccatc
     2461 gccgtgatgg cctcgggggt cacctcggcc tcgttcgcgg ccacccgggt cgggggccgc
     2521 tactggacgg gcgtgttcgg ggaagagaac ccgcccctga agctgatcgc cgtcctggtc
     2581 atcctgctgg tggcggccgt gaactaccgc ggggtgggag agtcgatcaa ggtcaacatc
     2641 gccatcaccc tgatcgagct gtccggcctg ctgttcatca tcctggtcgg cgtcaaggtg
     2701 ctgttcagcg gcgacggcga ccccggacag gcgttcgagt tcaaggactc cgggttcggg
     2761 gccctgaccg gcatcaccgc cggcgcggcc accgccttct acgccttcat cggcttcgag
     2821 gactcggtga acatggccga ggaggtccgc gaccccatca acaccttccc gccggccctg
     2881 ctcaccggca tcgtcgccgc cagcatcatc tacctgctgg tcggcttcac cgcggccatg
     2941 accgtgcccc tggacgtgct caccgagtcc accgggccgc tgctcgaggt ggtgcggatc
     3001 ggcctgcccg actacgacgc cggcaccctg ttctcggtca tcgccatgat cgcagtctcc
     3061 aacaccatgc tgatcaacat gctgatggcc tcgcggctgc tgtacggcat ggccaaccag
     3121 ggggtgctgc cggccccgtt cgcctgggtc tcgccccgca agacgccgtg gttcgggatc
     3181 ctgttcacca ccgccctgtc gatcctgctg atcctcaccg tcaacgacct ctccggcctg
     3241 tccgacacca ccgtgctcct gctgacctcg gtgttcctcc tggtcaacat ctgcgtgctg
     3301 gtgctgcggc gcgacccggt gggccacagc cacttcgtgg cgcccacgat catgccgatc
     3361 atcggggcgc tggtcgccct ggtcttcctg ctgcccatca accgcaccgg cgaggtctac
     3421 cgcaccgcca tctacctgct gatcggcggg ctggtcctgt gggggatc
//
LOCUS       DASSWR010000585         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1010872, whole genome shotgun sequence.
ACCESSION   DASSWR010000585 DASSWR010000000
VERSION     DASSWR010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010872"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFO47_09765"
     CDS             complement(<1..318)
                     /locus_tag="VFO47_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET8789021.1"
                     /translation="MDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVTELVTHDKAGERILESLLAAAEARLHEEGI
                     AGKI"
     gene            complement(328..2625)
                     /locus_tag="VFO47_09770"
     CDS             complement(328..2625)
                     /locus_tag="VFO47_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789022.1"
                     /translation="MTAAPPPPAAWPLVLAGPMPRRVEPGAVSVFVACRAPRTVELSI
                     YRGTRPGPERLVHAERGRTVPLGRWLHVAVVTAHPPAPLVPGTVYGYDLRFLREAGPA
                     DGDDGPAEAGLGSLGLLERLGYRPGELPSFVPPPERLEQVRIVHGSCRKPHGQRRDAL
                     ATLDTILAASHDDPEARPQQLFLTGDQIYADDVAPGLLDLCRRVGEAALGWERPEPLP
                     DTAGAGDDRLAPTRRDHLVRHHAGFTGPVLDCHLMPLAEFYGMYLLVWSEELWPRDPA
                     GLATLPAAEGEAGRSRAAVLELARTLPRVRRALANIATYMIFDDHDVTDDWNLHRRWH
                     DHVHGRPLGRRLVQNALSAYAVFQGWGNEPDQFAADRPGGQLLAALAAWDGQEGETCE
                     TIRARLGLPSAQAATPVRWDYQVDTPSFQAIVLDTRTQRGFRPGGNGLAAPALLGPEA
                     MARQLGDRLAARPGEVPVTVLVSAAPVFGHPLVEAKIQLKRIKAIEWHEQGPAAVDRE
                     AWSLDPAAFEALLAALVPFRRVVILSGDVHYGFAGSVAYWDRRDGVERQARFVQCTST
                     PLKNEDHRTRLLGGVPTVVQVPHLLGKRIERLLGRLTTPPSVSYLGWSSDPHPRGRRR
                     WRLGRRRFLPVPYVLPAPLAHAHRLAAPPEWGYQVDFQADAGTEPLVPGRSGAFHHRA
                     LALRAENRWSFMHCIVGRNNLGDIEFLPGVGGAPGMEPDLVRQSLWFDKTSIRSGSEP
                     ERLPYTIYDLPLAPPPPQGQPGRVR"
     gene            complement(2631..>3221)
                     /locus_tag="VFO47_09775"
     CDS             complement(2631..>3221)
                     /locus_tag="VFO47_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HET8789023.1"
                     /translation="ALLGVAVGGFGLVLRSDRAAARICRLAERAANPPRRLLRRPPAR
                     GWAEATTKFRRRARGLLRTRWPAITAAALISHLSLYLVLLVTLRHVGVGDAEIGWAQV
                     LFVFACGRLVTAVRFTPGGAGVVEVLLIGGLVVFGGDTDQVTAAVLVFRALTWLLPVP
                     LGALAYLAWRWRQHRRAAVGPEAAPVVPADPRARSG"
BASE COUNT          410 a         1174 c         1291 g          346 t
ORIGIN      
        1 gatcttgccg gcgatgccct cctcgtggag gcgggcctcg gcggcggcga gcaggctctc
       61 caggatccgc tcccccgcct tgtcgtgggt gaccagctcg gtcaccgagt cgcactcggg
      121 ggtggcgtac tcggggtggg agccgacgtc caggtacagc cgggccccgt tctccaggaa
      181 cacgttggag gagcgccccc aggagacgac ccgccggaac aggtagcggg ccacctcgtc
      241 cggtgacagc cggcgctggc cccggaacgt gcaggtgacg ccgtactcgt tctcgagccc
      301 gaagatccgc cggtccatct ggccatgcta ccggacgcgg cccggctggc cctggggcgg
      361 cggcggcgcc agggggaggt cgtagatggt gtagggcagc cgctcgggct cgctgcccga
      421 ccggatcgag gtcttgtcga accacagcga ctggcggacc aggtcgggct ccatgccggg
      481 ggcgccgccg accccgggca ggaactcgat gtcgccgagg ttgttgcgcc cgacgatgca
      541 gtgcatgaac gaccagcggt tctcggcccg cagggccagg gcccggtggt ggaacgcgcc
      601 cgaacggccc ggcaccagcg gctcggtgcc ggcgtcggcc tggaagtcga cctggtagcc
      661 ccactcgggc ggggcggcca gccggtgggc gtgggccagc ggcgccggca ggacgtacgg
      721 gaccgggagg aaccgccgcc ggcccagccg ccagcgccgc cggccccgcg ggtgggggtc
      781 gctcgaccag ccaaggtacg acaccgacgg gggcgtggtc aggcggccca gcagccgctc
      841 gatccgcttg ccgagcaggt gcgggacctg cacgacggtc ggcaccccgc ccagcagccg
      901 ggtgcggtgg tcctcgttct tcagcggggt cgaggtgcac tggacgaagc gggcctgccg
      961 ctccaccccg tcgcgccggt cccagtaggc gaccgacccg gcgaagccgt agtggacgtc
     1021 gccggacagg atcaccaccc gccggaacgg gaccagggcg gccagcaggg cctcgaacgc
     1081 ggccgggtcc agcgaccacg cctcccggtc gaccgcggcc ggcccctgct cgtgccactc
     1141 gatggccttg atccgcttga gctggatctt ggcctcgacc agagggtggc cgaacacggg
     1201 ggcggccgac accagcaccg tcaccgggac ctcgccgggg cgggcggcca gccggtcgcc
     1261 gagctggcgg gccatggcct cggggccgag cagggccggg gcggccagcc cgttgccgcc
     1321 cggccggaac ccccgctggg tccgggtgtc gagcacgatc gcctggaagc tgggggtgtc
     1381 gacctggtag tcccagcgga ccggggtcgc cgcctgggcc gatggcagcc ccagccgggc
     1441 ccggatcgtc tcgcaggtct cgccctcctg gccgtcccag gcggccaggg cggccagcag
     1501 ttggccgccc ggccggtcgg cggcgaactg gtccggctcg ttgccccagc cctggaacac
     1561 cgcgtaggcg ctgagggcgt tctggaccag gcgccggccc agcggccggc cgtggacgtg
     1621 gtcgtgccag cggcggtgca ggttccagtc gtcggtgacg tcgtggtcgt cgaagatcat
     1681 gtaggtggcg atgttggcca gggcccggcg cacccggggc agggtgcggg cgagctcgag
     1741 cacggccgcc cgggagcggc cggcctcccc ctcggccgcg ggcagggtgg ccagcccggc
     1801 ggggtcgcgg ggccacagct cctccgacca gaccagcagg tacatgccgt agaactcggc
     1861 cagcggcatc aggtggcagt ccagcaccgg gccggtgaag ccggcgtggt ggcgcaccag
     1921 gtggtcgcgg cgggtcgggg ccagccggtc gtcgccggcg ccggcggtgt cggggagcgg
     1981 ctcgggccgc tcccagccga gcgcggcctc cccgacccgc cggcagaggt cgagcagccc
     2041 gggggcgacg tcgtcggcgt agatctggtc gccggtcagg aacagctgct gcggcctggc
     2101 ctcggggtcg tcgtggctgg cggccaggat ggtgtcgagg gtggccaggg cgtcgcggcg
     2161 ttggccgtgc ggcttacggc aggacccgtg gacgatccgg acctgctcga ggcgctcggg
     2221 tggggggacg aagctgggca gctcgccggg ccggtagccg agccgctcga gcaggccgag
     2281 cgacccgagg ccggcctcgg cggggccgtc gtcgccgtcc gccgggccgg cctcgcggag
     2341 gaagcgcagg tcgtagccgt agaccgtgcc cgggacgagg ggcgccggcg ggtgggcggt
     2401 gaccacggcg acgtgcagcc agcggcccag cgggacggtc ctcccccgct cggcgtggac
     2461 cagccgctcc gggccgggcc gggtcccgcg gtagatcgac agctcgaccg tccgcggcgc
     2521 ccggcaggcc acgaacacgc tgaccgcccc cggttcgacc cggcgcggca tcggcccggc
     2581 cagcaccagt ggccaggcgg cggggggcgg gggcgcggcc gtcaccgggc tcaccccgac
     2641 cgggcgcgcg ggtcggcggg cacgaccggc gcggcctcgg ggccaacggc ggcccgccgg
     2701 tgctggcgcc agcgccaggc gaggtaggcg agggcgccca gggggaccgg cagcagccag
     2761 gtcagggccc ggaagaccag caccgcggcc gtcacctggt ccgtgtcgcc cccgaacacg
     2821 accagcccgc cgatgagcag cacctcgacc acgccggcgc cgccgggggt gaaccggacc
     2881 gcggtgacca gccggccgca ggcgaacacg aacagcacct gggcccagcc gatctcggcg
     2941 tcgccgacgc cgacgtggcg cagggtgacc agcagcacca ggtacagcga caggtggctg
     3001 atcagcgcgg ccgcggtgat ggccggccag cgcgtccgca gcaggccgcg ggcccggcgg
     3061 cggaacttgg tggtcgcctc cgcccagccg cgcgccgggg gccgccgcag cagccggcgg
     3121 ggcgggttgg cggcccgctc ggccagccgg cagatccggg ccgccgcccg gtcgctgcgc
     3181 agcaccagcc cgaagccgcc gacggccacc cccagcagag c
//
LOCUS       DASSWR010000586         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_530683,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000586 DASSWR010000000
VERSION     DASSWR010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530683"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFO47_09780"
     CDS             complement(<1..204)
                     /locus_tag="VFO47_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789024.1"
                     /translation="MGADRASPRTEEGNLSVTDRLVIRGAREHNLKNVHLDLPRNALI
                     VFTGLSGSGKSSLAFDTIYAEGQR"
     gene            267..923
                     /locus_tag="VFO47_09785"
     CDS             267..923
                     /locus_tag="VFO47_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8789025.1"
                     /translation="MPDFDPAHVDRGGPPHRYPGGPLEVTKLHVGPYENNAYLLRDPE
                     TNDALLVDAANEAERLLELLEGVTLAGIVTTHRHPDHIQALAGVLKVHDVWNGAHGAD
                     ADAIADQVGVTPDRVLEHGDTLTVGRYAVTVLHTPGHTEGSISFKLPSSQVLTGDALF
                     PGGVGKTEGPDAFAEAIRSAERHLLSLPGETRISPGHGDDTLVEREVPQLDEWKARGW
                     "
     gene            920..1336
                     /locus_tag="VFO47_09790"
     CDS             920..1336
                     /locus_tag="VFO47_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789026.1"
                     /translation="MTGARGLRAAAVALVLAVPVLAGCEAAQQAQEVGQGVDKAADCA
                     GLVGELTGINWSDVQQAPAEAEQAAQRLDERLREVNDADVKAAGEALRDRVRQLAEAA
                     QNADAADAQQALSDVEAAARRLANACGVNVDQVGGG"
     gene            complement(1342..>2328)
                     /locus_tag="VFO47_09795"
     CDS             complement(1342..>2328)
                     /locus_tag="VFO47_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET8789027.1"
                     /translation="EVRLVAKDGQGGEDGEATKVLYEFAGTDGEAVETTLDPQAQRAA
                     EAALDPVSKPAALVAVRPSTGELVAVVNTPFEGYNRALLGRYPPGSTFKVVTAGALLE
                     SGLRPTDPVDCPKEAKVGGRTFGNFEDEVLGRIPFSSAFAHSCNTAFVQQAAKRLDGD
                     ELVAAADRFGFGLDPSPGIPAVTSRVPAPSDRADLAAEAFGQGRVVASPLQMALVAAT
                     VADGRWRQPTLVAGEAAEADPPEPLDPKVATTLRTLMRQVVTEGTAAPAGLPGKTAGK
                     TGTAEFGTGDPLPTHAWFIGFRGDLAFSVVVEDGGVGGRVAAPVAARFLRGL"
BASE COUNT          316 a          864 c          877 g          271 t
ORIGIN      
        1 gcgctggccc tcggcgtaga tggtgtcgaa ggcaagcgag gacttgcccg acccggacag
       61 gcccgtgaac acgatcaggg cgttgcgggg caggtccagg tggacgttct tgaggttgtg
      121 ctcgcgtgcg ccccggatga cgaggcggtc ggtgacgctc aggttccctt cctcggttcg
      181 gggggaagct cgatcggccc ccacattgta tggacgcctg tgacaacaat gtcccctgac
      241 gctccgacat tccacaggag gcgcacatgc cggacttcga ccccgcccac gtcgaccggg
      301 gcggcccgcc gcaccgctac cccggcgggc ccctggaagt gaccaagctg cacgtcggcc
      361 cctacgagaa caacgcctac ctgctgcgcg accccgagac caacgacgcc ctgctggtcg
      421 acgcggccaa cgaggccgag cggctgctgg agctgctgga gggcgtcacc ctggccggga
      481 tcgtcaccac ccaccgccac cccgaccaca tccaggccct ggccggggtg ctcaaggtcc
      541 acgacgtctg gaacggcgcc cacggggccg acgccgacgc catcgccgac caggtcggcg
      601 tcacccccga ccgggtgctg gagcacggcg acaccctgac cgtcggccgc tacgcggtca
      661 ccgtcctgca cacccccggc cacaccgagg gcagcatctc gttcaagctg ccgtccagcc
      721 aggtcctgac cggcgacgcc ctgttcccgg gcggggtcgg caagaccgag gggcccgacg
      781 ccttcgccga ggcgatccgc tcggctgagc ggcacctgct gtcgctgccc ggcgagaccc
      841 gcatctcccc cgggcacggc gacgacaccc tggtcgagcg ggaggtcccc cagctcgacg
      901 agtggaaggc gaggggctgg tgaccggagc ccgcggcctc agggccgccg ccgtcgccct
      961 agtcctggcc gtgcccgtgc tggccggctg cgaggccgcc cagcaggccc aggaggtcgg
     1021 ccagggggtc gacaaggcgg ccgactgcgc cggcctggtc ggggagctga cggggatcaa
     1081 ctggagcgac gtgcagcagg ccccggccga ggccgaacag gccgcccagc ggctcgacga
     1141 gcggctccgc gaggtgaacg acgccgacgt caaggcggcc ggcgaggccc tgcgggaccg
     1201 ggtccgccag ctggccgagg ccgcccagaa cgccgacgcg gccgacgccc agcaggccct
     1261 gtccgacgtc gaggcggccg cccggcggct ggccaacgcc tgcggggtga acgtcgacca
     1321 ggtcggcggc ggctagggcg gctacaggcc gcggaggaac cgggccgcga ccggggcggc
     1381 gacgcggccg ccgacgccgc cgtcctcgac caccaccgag aaggccaggt cgccgcggaa
     1441 gccgatgaac caggcgtggg tgggcagcgg gtcgccggtg ccgaactcgg ccgtgccggt
     1501 cttgccggcg gtcttgccgg gcaggccggc cggggcggcc gtgccctcgg tgaccacctg
     1561 gcgcatcagg gtccgcaggg tggtggcgac cttggggtcc agcggctcgg gggggtcggc
     1621 ctcggccgcc tcgccggcga ccagggtggg ctggcgccag cggccgtcgg ccacggtggc
     1681 cgcgaccagg gccatctgca gggggctggc caccacccgg ccctggccga aggcctcggc
     1741 ggccaggtcg gcccggtccg agggggccgg cacccggctg gtcacggccg ggatgccggg
     1801 ggacgggtcg agcccgaacc cgaaccggtc cgcggccgcg accagctcgt cgccgtccag
     1861 ccgcttggcc gcctgctgca cgaaggcggt gttgcaggag tgggcgaagg cgctggagaa
     1921 cgggatccgg cccagcacct cgtcctcgaa gttgccgaag gtgcggccgc cgaccttggc
     1981 ctccttgggg cagtcgaccg ggtcggtcgg gcgcagaccg gactccagca gggccccggc
     2041 ggtcaccacc ttgaaggtcg acccgggcgg gtagcggccg agcagggccc ggttgtagcc
     2101 ctcgaacggg gtgttgacga ccgccaccag ctcgccggtg gacggccgca ccgccaccag
     2161 ggcggccggc ttgctgaccg ggtccagggc cgcctcggcg gcccgctggg cctgcgggtc
     2221 cagggtcgtc tccacggcct cgccgtcggt gccggcgaac tcgtagagga ccttggtcgc
     2281 ctcgccgtcc tcgccgccct ggccgtcctt ggcgaccagg cggacctc
//
LOCUS       DASSWR010000587         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_813334,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000587 DASSWR010000000
VERSION     DASSWR010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813334"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VFO47_09800"
     CDS             <1..1597
                     /locus_tag="VFO47_09800"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8789028.1"
                     /translation="VELGMADRLAARAVAEAAGAPVEQVLAGARDTGDLGLTAEQLLE
                     ELEPGRPATRAVERVFEGLHEIAEAQGTGSQGRKLAGMVGLLRQATPLEARYLLRTVT
                     ANLRLGIGTATILDALAEVHAGGRKQRSILERAYNICSDLGLVAATLVTEGLAAVEAM
                     EVRAGNPVRPMLAQRLGEPAEILAKLGGSCAAEYKYDGIRVQAHRTADGALELYTRRL
                     DRVSTQFPEVVQLLSESLKPREVILEGEVVAFDPASGELRPFQDVMFRRRKYGIAEAT
                     KDFPVSMFCFELLYADGTDLTRLPYLERRARLAEAITPSARLRLATAEQVGDEAAMER
                     VFEQAVAEGCEGVICKSVAPTAQYQAGARGWQWIKLKRDYRSELTDTLDLVVVGGLAG
                     RGRRAGMYGALLLGVYDPAGDRFQTICKCGTGFSDAELAALPARMAPLVRAQRPARVD
                     SRWHADVWFEPTLVVEVLAAEITLSPHHTAGWGLLKEDAGLALRFPRFTGRWRDDKAP
                     TDATTVDEVVTMYRAARRAPADA"
     gene            complement(1616..>2027)
                     /locus_tag="VFO47_09805"
     CDS             complement(1616..>2027)
                     /locus_tag="VFO47_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789029.1"
                     /translation="SQPAEPLGRGPASGAADGQTGSQSGGAEQQEEPTGIEHQPHGVM
                     QSLAGEVSGSAQLLGAEQNAKYCQDGSQHPAVTVSNQGATQPEQSRITRHARTLLALA
                     ALNELWPPLLLRRVILQESRTGRATDLTSGDRPA"
BASE COUNT          268 a          733 c          749 g          277 t
ORIGIN      
        1 ggtcgagctg gggatggccg accggctggc cgcccgggcg gtcgccgagg ccgccggcgc
       61 gcccgtcgag caggtcctgg ccggcgcccg ggacaccggc gacctgggcc tgaccgccga
      121 acagctgctg gaggagctgg agcccggccg gccggccacc cgtgcggtgg agcgggtgtt
      181 cgagggcctg cacgagatcg ccgaggccca gggcaccggc tcccagggcc gcaagctggc
      241 cgggatggtc ggcctgctcc ggcaggccac gccgctggag gcccgctacc tgctgcgcac
      301 ggtcacggcc aacctgcggc tggggatcgg gaccgccacc atcctggacg ccctggccga
      361 ggtccacgcc ggcggccgca agcagcgatc gatcctggag cgggcctaca acatctgctc
      421 cgacctcggc ctggtcgcgg ccacgctcgt caccgagggc ctggccgcgg tcgaggcgat
      481 ggaggtgcgg gccgggaacc cggtgcggcc gatgctggcc cagcgcctcg gcgaaccggc
      541 cgagatcctg gccaagctgg gcgggtcgtg cgcggccgag tacaagtacg acgggatccg
      601 ggtccaggcc caccgcaccg ccgacggcgc ccttgagctg tacacgcggc ggctggaccg
      661 ggtctcgacc cagttccccg aggtggtgca gctgctctcc gagagcctga agccgcgcga
      721 ggtgatcctg gagggcgagg tggtggcctt cgacccggcc tccggcgaac tgcgcccctt
      781 ccaggacgtg atgttccgcc gccgcaagta cggcatcgcc gaggcgacca aggacttccc
      841 ggtgagcatg ttctgcttcg agctgctgta cgccgacggg accgacctga cccgcctgcc
      901 ctacctggag cggcgcgccc ggctggccga ggcgatcacg ccgtcggcgc ggctgcggct
      961 ggccaccgcc gagcaggtcg gcgacgaggc ggccatggag cgggtgttcg agcaggcggt
     1021 ggccgagggc tgcgaggggg tgatctgcaa gtcggtcgcc ccgacggcgc agtaccaggc
     1081 cggggcccgg ggctggcagt ggatcaagct caagcgcgac taccgcagcg agctcaccga
     1141 cacccttgac ctggtcgtgg tcggcggcct ggccggacgg ggccgccgcg ccgggatgta
     1201 cggggcgctg ctgctcggcg tctacgaccc ggccggcgac cggttccaga cgatctgcaa
     1261 gtgcggcacc ggcttctccg acgccgagct ggccgcgctg ccggcccgga tggcgccgct
     1321 ggtgcgggcg cagcggccgg ccagggtgga ctcacgctgg cacgccgacg tgtggttcga
     1381 gcccaccctg gtggtcgagg tgctggccgc cgagatcacc ctgtcgcccc accacaccgc
     1441 cgggtggggg ctgctcaagg aggacgccgg cctggccctg cggttccccc gcttcaccgg
     1501 ccgctggcgc gacgacaagg cccccaccga cgccaccacc gtggacgagg tcgtaaccat
     1561 gtaccgcgcc gcccggcggg cccccgccga cgcctaaccg cggcaatatc cacggtcaag
     1621 caggccggtc cccagaggtg aggtcggttg ctcgtcccgt ccttgattcc tgcagaatca
     1681 cccgacggag cagaagcggc ggccagagtt cgttcaacgc tgccaatgca aggagggtcc
     1741 tggcatggcg cgtgatcctc gactgttcgg gctgcgtcgc gccttggtta ctcaccgtaa
     1801 cggctgggtg ctggctgccg tcctggcaat acttggcatt ctgctcggcg cccagcagct
     1861 gagccgagcc gctgacctcg ccggccaggc tctgcatgac gccatgcggc tggtgctcga
     1921 tgccggttgg ctcctcctgt tgctcggcgc cgccggactg gctgcccgtc tggccatcag
     1981 ccgcccccga ggccgggcca cggcccagag gctccgctgg ctgcgag
//
LOCUS       DASSWR010000588         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_530836,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000588 DASSWR010000000
VERSION     DASSWR010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530836"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..677)
                     /gene="def"
                     /locus_tag="VFO47_09810"
     CDS             complement(99..677)
                     /gene="def"
                     /locus_tag="VFO47_09810"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET8789030.1"
                     /translation="MAVLEIRLYGDPVLTQRSDRVTRFDAELARFGDDLLETLRAAHG
                     AGLAAPQVGVLKRMFAYDIPPDEETGEHPFGVLVNPVITRWEGEQDGEEGCLSFPGLY
                     YACKRAMVVTASAVDVRGRPLELTGEGLLARCIQHEIDHLDGVLFIDRMSRGDRKRAL
                     KQWREREFDLELDGIAPHLHRSDLTIKPDKTL"
     gene            complement(695..>2110)
                     /locus_tag="VFO47_09815"
     CDS             complement(695..>2110)
                     /locus_tag="VFO47_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789031.1"
                     /translation="HPDLDAADPLVAMVSGGRAGAVYWRPLPGEDRGARVIALIEAAL
                     AGGRGAIVVTPEVAAGSAVGDAVRKAFPDAADLASDLSDRRRYRAWAELRRGRRLVVI
                     GGRSSVLAPLPALGCVVVDDEANFAHKEQRTPRFHARDVALRRAAAARALCVLTGTVP
                     SAEAVAAMQAGQCRLLAPDRAAERAARPLVEVVDPDDEGPAAARLHPRGLAAIRAALA
                     RDEPAYVLVPRRGGADPAAPGARTAGQVAAELARILPGVPVWRLDREVVDPGMVPPWA
                     GQRPGVVVGTVAGVKDHPPLTGCRTVVVVGADTALGQAEIRSAEEAYRTWSRAATWCG
                     PRGGAGRLVLQTRHGGHHAVQALVRWDPEFFWRHELPRRVELGFPPARRLVLVEGPEP
                     EEADAAMAALAAAFGPKVELLGPAAMGRGWRIIAKVEDAESAARALRPLLTEASRAGG
                     PRMSVDVEPLEVLAAPRQHPG"
BASE COUNT          248 a          831 c          805 g          226 t
ORIGIN      
        1 ccaccgccac cacctcgtgg gcggacccga gcagcgcctg cagcggcggc acggccgccg
       61 ccggcgtgcc caggtagacg acgcgcaccg tccgacccct agagggtctt gtcgggcttg
      121 atggtgaggt ccgaccggtg caggtgcggg gcaatgccgt cgagctcgag gtcgaactcg
      181 cgctcgcgcc actgcttgag ggcccgcttg cggtcgcccc gcgacatccg gtcgatgaac
      241 agcaccccgt ccaggtggtc gatctcgtgc tggatgcagc gggccagcag cccctccccg
      301 gtcagctcca gcggccggcc gcggacgtcg acggccgacg ccgtgacgac catggcccgc
      361 ttgcaggcgt agtagaggcc ggggaacgac aggcagccct cctcgccgtc ctgctcgccc
      421 tcccagcggg tgatgaccgg gttgaccagc accccgaacg ggtgctcgcc ggtctcctcg
      481 tcgggcggga tgtcgtaggc gaacatgcgc ttgagcaccc cgacctgtgg cgcggccagg
      541 ccggcgccgt gggcggcccg gagggtctcc agcaggtcgt cgccgaagcg ggccagctcg
      601 gcgtcgaacc gggtcacccg gtccgaccgc tgggtcagga ccgggtcgcc gtacagccgg
      661 atctcgagca ctgccacgtg acgcgtctcc tcgttcagcc agggtgctgg cggggggcgg
      721 cgaggacttc gaggggctcg acgtccaccg acatgcgcgg gccgccggcc cgggatgcct
      781 cggtgagcag gggccggagg gccctggccg ccgactcggc gtcctcgacc ttggcgatga
      841 tacgccagcc cctgcccatg gccgccggcc cgagcagctc gaccttgggc ccgaaggcgg
      901 cggccagggc ggccatggcc gcgtcggcct cctccggctc cggcccctcg accaggacca
      961 gccgccgggc cggggggaag ccgagctcga cccggcgggg cagctcgtgg cgccagaaga
     1021 actccgggtc ccagcggacc agggcctgga cggcgtggtg gccgccgtgg cgggtctgga
     1081 ggacgaggcg gccggcgccg ccccgggggc cgcaccaggt ggcggcccgg ctccaggtcc
     1141 ggtaggcctc ctcggccgag cggatctcgg cctggccgag ggcggtgtcg gcgccgacca
     1201 cgaccacggt gcggcagccg gtcagcggcg ggtggtcctt gaccccggcc acggtgccga
     1261 cgaccacccc gggccgctgc ccggcccagg ggggcaccat gcccgggtcg accacctccc
     1321 ggtccagccg ccacaccggc accccgggca ggatgcgggc cagctcggcc gcgacctgcc
     1381 cggcggtgcg ggccccggga gcggccgggt cggccccgcc gcgccgcggc accagcacgt
     1441 aggcgggctc gtcgcgggcc agggcggccc ggatggcggc caggccgcgg gggtggaggc
     1501 gggccgcggc cgggccctcg tcgtcggggt cgaccacctc gaccaggggg cgggcggccc
     1561 gctcggccgc ccggtcgggg gcgagcagcc ggcactggcc ggcctgcatg gcggccacgg
     1621 cctcggccga gggcacggtg ccggtgagca cgcacagggc ccgggcggcg gcggcccggc
     1681 gcagggcgac gtcgcgggcg tggaagcggg gggtgcgctg ctccttgtgg gcgaagttgg
     1741 cctcgtcgtc cacgaccacg cacccgagcg cgggcagcgg ggcgagcacg ctggagcgcc
     1801 ccccgatcac gaccagccgc cgcccccgcc gcagctcggc ccaggcccgg taccgccgcc
     1861 ggtccgacag gtcgctggcc aggtcggccg cgtccgggaa cgccttgcgc acggcgtccc
     1921 ccaccgccga ccccgccgcc acctccggcg tcaccacgat cgctccccgc cccccggcca
     1981 gcgccgcctc aatcagtgcg atcaccctcg ccccccggtc ctcccccggc agcggccgcc
     2041 agtacaccgc ccccgcccgc cccccgctga ccatcgcaac cagcgggtcg gcggcgtcca
     2101 gatcggggtg
//
LOCUS       DASSWR010000589         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1293978, whole genome shotgun sequence.
ACCESSION   DASSWR010000589 DASSWR010000000
VERSION     DASSWR010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293978"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFO47_09820"
     CDS             complement(<1..970)
                     /locus_tag="VFO47_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET8789032.1"
                     /translation="MAQNRPSPGLAREAIGLREVLFQSITHMAPAAAVAFSIIVGANF
                     AAGALPLSVIFALVGCLLVAVSIGQLARRLPSAGGFTTYAARGLHPAVGFLVGWGYAF
                     VEPLVAPLLFLIFGNVVASTLNQEFGWSYDTWWVVSAVFAAVVVFVLGWFGVRLSTGA
                     GTLLGLFEILVFAALAVTLIFQAGDANTLSVFGTGFADVEGFTGLSGVVAGSVYTILA
                     FIGFEAAAPLAEEAKDPRRTIRQAVIYSCLAIGVFYVLTTYAATVSFGPDRFAEFPAS
                     GDGNPWDALARGAWGAGWVLVFLAIANSAIANANASANAATRTWFAM"
     gene            1154..1921
                     /locus_tag="VFO47_09825"
     CDS             1154..1921
                     /locus_tag="VFO47_09825"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET8789033.1"
                     /translation="MGRLEGKVAVITGAASGIGRESARRFAAEGALVCVADLADEAGE
                     AVADEVGGLYLHADVTDEDDVVAMYAKAAERYGGIDVLFNNAGIAPPDDVSVLETSLE
                     AWQRVQDVNLKAVFLCCKHGIPHLLARGGGSVVNTASFVAVMGAATSQISYTASKGGV
                     LALSRELGVEFGRRGVRVNALCPGPVNTPLLQELFAKDPEKAARRLVHLPMGRFAEAA
                     EIAAAACFLASDDASYITASTFLVDGGLSGAYVTPLE"
BASE COUNT          343 a          677 c          763 g          241 t
ORIGIN      
        1 ccatggcgaa ccaggtccgg gtggccgcgt tggccgaggc gttggcgttg gcgatggccg
       61 agttggcgat ggccaggaac accagcaccc agccagcgcc ccaggcccca cgggccaggg
      121 cgtcccaggg attgccgtcc cccgaggccg ggaactcggc gaaccggtcc gggccgaagg
      181 acacggtggc cgcataggtg gtcagcacgt agaacacgcc gatggccaga caggagtaga
      241 tgaccgcctg ccggatggtg cgccgcgggt ccttggcctc ctcggccagg ggggccgcgg
      301 cctcgaagcc gatgaaggcc aggatcgtgt agacggagcc ggcgacgacc ccggagaggc
      361 cggtgaagcc ctcgacgtcg gcgaagccgg tgccgaacac ggaaagggtg ttggcgtcgc
      421 cggcctggaa gatcagggtg acagccaggg ccgcgaacac gaggatctcg aacagcccca
      481 gcagcgtgcc cgcgccggtg ctcagccgca ccccgaacca cccgaggacg aagaccacca
      541 cggccgcgaa caccgccgac accacccacc aggtgtcgta gctccagccg aactcctggt
      601 tcagggtgct ggccacgacg ttgccgaaga tgaggaacag cagcggcgcc accaggggct
      661 ccacgaacgc gtagccccag ccgaccagga agccgacggc cgggtgcagg ccgcgggcgg
      721 cgtaggtggt gaagccgccg gccgacggca ggcgccgggc cagctgcccg atcgagaccg
      781 ccaccagcag gcagccgacc agggcgaaga tcactgacag gggcagggcc ccggcggcga
      841 agttggcccc gacgatgatc gagaaggcga ccgcggccgc cggggccatg tgggtgatcg
      901 actggaacag gacctcgcgg agaccgattg cttcccgggc gaggccgggg gacggacggt
      961 tctgcgccat ggcgtgcctc cttcggagac ctcccaccac caggacaggc acgccgaacg
     1021 gtgaaccgac gccatcttcc ttgcaagtgg ctcgaaagcg acgagcagcg ccatcgggcc
     1081 gagggtgcag gcggaaccgc ggccacggcg acatactggg ccggctcatc ggacacagag
     1141 gaggtcggtc gagatcggtc ggctcgaggg gaaggtggcc gtcatcacgg gggcggccag
     1201 cgggatcggg cgggagagcg cgcggcggtt cgcggccgag ggggcgctgg tgtgcgtggc
     1261 cgacctggcg gatgaggccg gggaggcggt ggcggacgag gtcggcgggc tgtacctgca
     1321 cgccgacgtg accgacgagg acgacgtcgt cgccatgtac gccaaggcgg cggagcgcta
     1381 cggcgggatc gacgtgctgt tcaacaacgc cggcatcgcc ccgccggacg acgtgtcggt
     1441 gctcgagacc agcctggagg cgtggcagcg ggtccaggac gtgaacctca aggcggtgtt
     1501 cctctgctgc aagcacggca tcccccacct gctggcccgc gggggcgggt cggtggtcaa
     1561 cacggcctcg ttcgtggccg tgatgggggc ggcgacctcg cagatctcct acaccgcgtc
     1621 caagggcggg gtgctggccc tctcacgcga gctgggtgtc gagttcgggc ggcggggagt
     1681 gcgggtcaac gccctctgcc ccggcccggt caacaccccg ctgctccagg agctgttcgc
     1741 caaggacccg gagaaggcgg cccgccgcct cgtccacctg cccatgggcc gcttcgccga
     1801 ggcggcggag atcgccgccg ccgcctgctt cctggcctcc gacgacgcca gctacatcac
     1861 cgccagcacg ttcctggtcg acggcggact gtccggcgcg tacgtgaccc cgctcgaata
     1921 ggaggagtcc ggtgaaggaa ggcaccatgg tcgagagggt ccaggaggcc cttgcccagc
     1981 tcgggatcga cgacgaggtg gtcgccgccg ggatgttctt cccc
//
LOCUS       DASSWR010000590         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_925663,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000590 DASSWR010000000
VERSION     DASSWR010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925663"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VFO47_09830"
     CDS             complement(<1..1955)
                     /locus_tag="VFO47_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET8789034.1"
                     /translation="MALATVTTLRSRTPASKILPPALPAAWVDRPELLRRLDDVGARR
                     LVTVVAGAGFGKSTLLAAWVARVHAAWYSLQPEDASLPNFLRGLVDALRLRLPNLPDE
                     LGTALASSRGPDAEKLAPADGFAAALGQALELQLSRDLVLVLDDVHELPTGGPSARLV
                     EGLARHAPARFHLVLSSRAEPPFPIQRLRGRGQVLEVDGGLLALGMEETGELLAAALG
                     PDGRELSEPVHRMTGGWPAAICLAVESLRHQRPPERAASLAALRRPGGLLFGYLAEEV
                     LAREDPVLRELVRWVAPLRRFTAELCRAIGVDADAGMLARLARRGLFVQPRSTVEGWF
                     VLNDLVREVVLASMPLEPGELRVLHRRAAAWLATGGHLEEALASVVACDDPPATAAFL
                     VEHGPAMLASGATDGVVAAAAGLPAELRSAAVEQVEGEARQVKGDWQGALECFRRAAG
                     EKRLLAPGLAWRTGLIHYMRSELDQALEAFRRARLDGALDADQALLLAWTATVHFLWN
                     QVDACREAAERALAAATACGDDRALAAAHTAMAMLAALEGDRAANDAHYLRALQAAER
                     AGDVLHLARIRANLGSRSTEEGSYREALVELEAAVRLAELSGYAAFLALAQTNRGEAQ
                     LGLGRLDEAIAELEAAKVTYQRMGSHKV"
BASE COUNT          312 a          833 c          787 g          254 t
ORIGIN      
        1 accttgtggc tgcccatccg ctggtaggtc accttggccg cctccaactc ggcgatggcc
       61 tcgtcgaggc ggccgagccc gagctgggcc tcgccccggt tggtctgggc cagggccagg
      121 aaggcggcat agccggacaa ctcggccagt cgcaccgcag cctccagctc gaccagcgcc
      181 tcccggtagg aaccctcctc ggtgctgcgc gagcccaggt tggcgcggat gcgggccagg
      241 tgcaggacgt cgccggcccg ctcagccgcc tgtagcgcac gcaggtagtg ggcgtcgttg
      301 gcggcccggt cgccctccag cgccgccagc atcgccatgg cggtgtgggc ggcggcaagc
      361 gcgcggtcgt caccgcaggc cgtggccgcg gccagcgccc gctccgccgc ctcgcggcag
      421 gcgtcgacct ggttccacag gaagtgaacc gtggccgtcc aggccagcag cagcgcctgg
      481 tcggcgtcga gcgcgccgtc cagccgcgcc cgccggaagg cctccagggc ctggtcgagc
      541 tcggagcgca tgtagtggat caggccggtg cgccacgcca gccccggggc cagcaaccgc
      601 ttttcgccgg cggcccggcg gaagcactcc agggcacctt gccagtcgcc cttgacctgg
      661 cgggcctcgc cctcgacctg ctcgaccgcg gcgctgcgca gctcggccgg caggccggcc
      721 gcggccgcca ccaccccgtc ggtcgcgccg gaggcgagca tggcggggcc gtgctcgacc
      781 aggaaggcgg ccgtggccgg tgggtcgtcg caggccacca ccgacgccaa tgcttcctcc
      841 aggtggccgc cggtggccag ccaggccgcc gcccgccggt gcagcacccg cagctcgccc
      901 ggctcgagcg gcatcgaggc cagcaccact tcgcggacca ggtcgttgag gacgaaccag
      961 ccctcgacgg tggagcgggg ctgcacgaac aggccgcgcc gggccagccg ggctagcatc
     1021 ccggcgtcgg cgtccacgcc gatggcgcgg cacagctcgg cggtgaagcg gcgcagcggc
     1081 gcaacccagc ggaccagctc ccgcaggacc gggtcttcac gcgccagcac ctcctcggcc
     1141 agatagccaa agagcagtcc gccgggccgg cgcagagccg ccaggctggc cgcccgctcc
     1201 ggtgggcgct ggtgacgcag cgactcgacc gccaggcaga tcgccgccgg ccagccgccg
     1261 gtcatccggt gcacaggctc gctcagctcg cgcccatccg ggccgagcgc ggccgccagc
     1321 agctcgccgg tctcctccat gccgagcgca agcaggccgc cgtcgacctc cagcacctgg
     1381 ccgcggccac gcagtcgctg gatcgggaac ggcggctcgg ccctcgacga cagcacgagg
     1441 tggaagcgag cgggcgcgtg tctggccagc ccctcgacca gccgagcgga cggcccgccg
     1501 gtaggcagct cgtggacgtc gtcgaggacc aggaccagat ctctgctcaa ctgaagctcc
     1561 agggcctggc cgagcgcagc cgcgaagccg tcggcaggag ccagcttctc ggcgtcgggc
     1621 ccgcgcgagc tcgccagcgc cgtgcccagc tcatccggga ggttcggcag gcgcagacgc
     1681 agcgcgtcaa ccagcccacg taggaagttg ggcaggctgg cgtcctccgg ctgcagcgag
     1741 taccaggccg cgtggacacg cgccacccag gcagccagca gggtcgactt gccgaagccg
     1801 gccccggcga cgacggtcac aaggcggcgg gcgccgacat cgtcgaggcg gcgcagcagc
     1861 tcaggccggt cgacccaggc ggccggcagc gccggcggca ggatcttgct cgccggcgtg
     1921 cgggaccgca acgtggtcac cgtggccagc gccaccctcc tcgtgccgag tctggatgga
     1981 cgagtctctc ccagcggcgt ccctacgcca agaggttgcc gtcttgtcca gccggcgaac
     2041 aggtcagccc gcctagggct ttatccgcag aagacgtcgt cgaagcttca tcctggaccg
     2101 ctacggcacc tgtacccgga agcggacttt caggcggccc gtgcagcgag caggcggcgc
     2161 atcgcgacga tgccggcgag cggccc
//
LOCUS       DASSWR010000591         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1207809, whole genome shotgun sequence.
ACCESSION   DASSWR010000591 DASSWR010000000
VERSION     DASSWR010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207809"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2739
                     /locus_tag="VFO47_09835"
     CDS             <1..2739
                     /locus_tag="VFO47_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8789035.1"
                     /translation="IIVGSLVVGDTLDHSFKQNAYRYLGVVDEVVSSPDPAQGEAAAR
                     RLETLRGDPAIDGLLTVRRHGAAVTKGGKGEPQATVLELDFAAAARFEGPGSPLAGPG
                     PGPGRVVLSDALAGALDARAGDRLTFHLYGRPLELEVARVVPARGLAGFGERGALVEP
                     GTIGRVAGPAGPRAGPETLTLVSNAGGVEGGAAASDQVKGKLQAALGPLARQGTAVAT
                     PKRELLDEAELIGAEFGALFLFVGSFAIIAGVLLLVNVFVMLAEERKSELGMLRAVGM
                     RRGRLVRGFVIEGTVYALVAAALGLVAGLGVGWAVVEVTARIFAGFTEEGVPELAFTV
                     TSTSLVNGFAAGFLIAFLTVALTSVRISRVNIIAAIRDLPPGTGRPLKRRWVLASTLA
                     AAGFGAAAVVAVAGSDGVGTYLYPALAVAALCPLLVRLLPRRAVYTGASLAVLGWGLV
                     ANTVRPELLDNSSTATFVVLGVVLTFSAVLLVSQNQELLLRLGRPLLARPSASGLSAR
                     LAVAYPVARRFRTGATLSMYGLVVFTLVLISVLGTVINAGVDRAVATASGGYALRVDW
                     NSQAPVTDPAARLAGGRFAGQVAGVAPLETAEAEVAGVPGAAKAVPAAAVGATAALAT
                     HGLFPLDARLDRLGDDRAVWRAVLADPRYVVVDQYLGYEESGMEAETWHPGDTLTLTD
                     PGTGRSERKTVAGVLADSTAFEGGGELPASPLVLAAGGLREQFGARARPSGALLALAP
                     GVSDRAVAADLQGEFLPQGLVATRMRHVVEQDYAANRSFFQLLQGFLALGLVVGVAGL
                     GVVMVRAVRERRRTVGVLRALGFPARVVRRAFLLESTFVALEGILLGTTLSIVTSYLL
                     FRNDDELQATGVAFPIPWMSISVLVLVTAAASLAATAWPARQAARISPAVALRIAD"
     gene            complement(2736..>2849)
                     /locus_tag="VFO47_09840"
     CDS             complement(2736..>2849)
                     /locus_tag="VFO47_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET8789036.1"
                     /translation="PVTGPVLRLITCGGAFDRSSGHYLDNVVVTARPAGAS"
BASE COUNT          284 a         1104 c         1066 g          395 t
ORIGIN      
        1 atcatcgtcg gcagcctggt cgtcggcgac accctcgacc actccttcaa gcagaacgcc
       61 taccgctacc tgggcgtggt cgacgaggtg gtcagctcgc ccgacccggc ccagggcgag
      121 gcggcggccc gtcggctcga gaccctgcgc ggcgaccccg ccatcgacgg cctcctcacc
      181 gtccgccgcc acggcgccgc cgtgaccaag ggcggcaagg gcgagccgca ggccaccgtg
      241 ctcgagctcg acttcgcggc cgcggcccgc ttcgagggcc cggggtcgcc cctggccggg
      301 cccggccccg ggccaggccg ggtggtcctg agcgacgccc tggccggcgc cctcgacgcc
      361 cgcgccggcg accggctcac cttccacctg tacggccgcc cgctggagct ggaggtggcc
      421 agggtcgtgc cggcgcgggg gctggccggg ttcggcgagc gcggcgccct ggtcgagccg
      481 ggcaccatcg ggcgggtggc cgggcccgcc ggcccccggg ccgggcccga gaccctcacg
      541 ctggtctcca acgccggcgg ggtcgagggc ggcgccgccg ccagcgacca ggtcaagggc
      601 aagctccagg ccgccctggg gccgctggcc cgccagggca cggccgtcgc cacccccaag
      661 cgggagctgc tggacgaggc cgagctgatc ggagccgagt tcggcgccct gttcctgttc
      721 gtcggcagct tcgccatcat cgccggcgtg ctcctgctgg tgaacgtgtt cgtgatgctg
      781 gccgaggagc gcaagagcga gctcggcatg ctgcgggcgg tcggcatgcg ccggggccgg
      841 ctggtccgcg gcttcgtgat cgagggcacc gtgtacgcgc tcgtcgccgc cgccctcggc
      901 ctggtcgccg ggctgggcgt cggctgggcc gtggtcgagg tcacggcccg gatcttcgcc
      961 ggcttcaccg aggagggcgt gcccgagctg gccttcaccg tcacctcgac cagcctggtc
     1021 aacgggttcg cggccggctt cctgatcgcg ttcctgaccg tcgccctgac cagcgtccgc
     1081 atcagccggg tcaacatcat cgccgccatc cgcgacctgc cgcccgggac cggccgcccg
     1141 ctcaagcggc gctgggtgct ggcctcgacc ctggccgcgg ccgggttcgg ggcggccgcg
     1201 gtggtcgccg tcgccggcag cgatggggtc ggcacctacc tgtacccggc cctggccgtg
     1261 gccgcgctgt gcccgctgct ggtccggctg ctgccccggc gggccgtcta caccggcgcg
     1321 tcgctggccg tgctcggctg ggggctggtc gccaacaccg tccggcccga gctgctcgac
     1381 aacagctcca ccgccacctt cgtggtcctt ggggtggtgc tgaccttctc ggccgtgctg
     1441 ctggtcagcc agaaccagga gctgctgctg cgcctggggc ggccgctgct ggcccgcccg
     1501 tcggcctccg gcctgtcggc ccgcctggcc gtcgcctacc cggtggccag gcgcttccgg
     1561 accggggcca ccctcagcat gtacggcctg gtggtgttca ccctggtgct gatctcggtg
     1621 ctggggacgg tgatcaacgc gggcgtggac cgggcggtgg cgaccgcctc cggcggctac
     1681 gccctccggg tcgactggaa cagccaggcg ccggtcaccg acccggcggc ccgcctggcc
     1741 gggggccgct tcgccggcca ggtggccggg gtcgcgcccc tggagacggc cgaggccgag
     1801 gtggccggcg tgcccggcgc ggccaaggcc gtcccggcgg cggccgtcgg ggccaccgcg
     1861 gccctggcca cccacggcct gttccccctg gacgcccggc tggaccgtct cggcgacgac
     1921 cgggccgtct ggcgcgcggt cctggccgac ccgcgctatg tggtcgtcga ccagtacctg
     1981 ggctacgagg agagcgggat ggaggccgag acctggcacc cgggcgacac cctgaccctg
     2041 accgacccgg gcaccggccg ctccgagcgc aagaccgtcg ccggcgtcct ggccgactcg
     2101 accgcgttcg agggcggcgg cgagctgccc gccagcccgc tggtgctggc cgccggcggg
     2161 ctccgggagc agttcggggc cagggcgcgg ccgtcggggg cgctgctggc gctcgccccc
     2221 ggcgtctccg accgggccgt tgccgccgac ctccagggcg agttcctgcc ccagggcctg
     2281 gtcgcgaccc gcatgcggca cgtggtcgag caggactacg ccgccaaccg ctcgttcttc
     2341 cagctcctcc agggcttcct ggccctcggc ctggtcgtcg gcgttgccgg cctgggcgtg
     2401 gtcatggtcc gggccgtgcg cgagcgccgc cgcaccgtcg gcgtgctgcg ggccctcggc
     2461 ttcccggccc gggtcgtccg gcgggcgttc ctgctggaga gcaccttcgt cgccctcgag
     2521 ggcatcctgc tcggcaccac cctgtcgatc gtcaccagct acctgctgtt ccgcaacgac
     2581 gacgagctgc aggcgaccgg ggtcgcgttc ccgatcccct ggatgtcgat ctcggtgctg
     2641 gtcctggtca cggcggccgc ctccctggcc gccaccgcct ggccggcccg ccaggcggcc
     2701 aggatcagcc cggcggtggc cctgcggatc gccgactagg aggcaccggc cggccgggcg
     2761 gtcacgacca cgttgtccag gtagtggccg ctcgaccggt cgaaggcgcc gccgcaggtg
     2821 atcaggcgca ggaccgggcc ggtgaccgg
//
LOCUS       DASSWR010000592         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_925701,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000592 DASSWR010000000
VERSION     DASSWR010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925701"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFO47_09845"
     CDS             <1..958
                     /locus_tag="VFO47_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789037.1"
                     /translation="AMSGPMNQVRTVLGDVPSERLGMCFAHEHLVIDGGVAKLVNPEI
                     SLQRVDDAVAELRPCVAAGLRAVVDAMPADAGRNVVKLAAISRRAGVHVVAATGLHHA
                     RFYGERHWGELLAPGELAELFVAELAEGIDANDLNGPVVRRTPHRAGVVKVAGSLGGP
                     SDRDRRAFEAAAMATARVGAPLLTHCEQGTGGVAQLRLLEELGVPLDRVILSHTDKVA
                     DRGYHRELLTSGAYLVYDQGIRTPEATARLVRWMVSDGHGDRLLLGTDGARRSLWSVL
                     GGTPGLAALRTHLGKRLADELGQAAMDRIWVANPAAALALRP"
     gene            955..1728
                     /locus_tag="VFO47_09850"
     CDS             955..1728
                     /locus_tag="VFO47_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8789038.1"
                     /translation="MSGAGRLAGKVCVVTGASGMAADAARRFAREGAQVWVVARDRDQ
                     CEALGLPYAVADLRDEGETGAAFAALRRAHPRVDALYAVAGASGRPLGDGPAHELTLA
                     AWEGTLALNATPAFLSAREALRAMLDQPPGPSATRGSLVLMSSVLAGHPMAPLFATHA
                     YAAAKAATIGLVRAMAAYYGEHAIRVNALAPSLVRTPMSARAAADPASVAAAERRQPL
                     TGGFLEAADVTEAAVYLCSDESRAVTGQVLAVDGGWTVT"
     gene            1768..2472
                     /gene="deoC"
                     /locus_tag="VFO47_09855"
     CDS             1768..2472
                     /gene="deoC"
                     /locus_tag="VFO47_09855"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET8789039.1"
                     /translation="MPDRWTVETITEEKVAKTIDHSLLRPELTIGEVLAGCELAAAYD
                     VASVCCRPLDVGRCRDALAGSAVAVGTVVGFPHGSSTTAVKAAEAARALDDGATELDM
                     VLPIGWLRSGEDAYVHDDIAAVVAVARPRGAVVKVILENAYLTDEEKVRGCELTEKAG
                     ADYVKTSTGYAPSGSTMDDLRLMRASVSERVKVKAAGGIRTLDLLIEGLNAGMDRCGA
                     TATAAIIDDLRARQGS"
     gene            2469..>2811
                     /locus_tag="VFO47_09860"
     CDS             2469..>2811
                     /locus_tag="VFO47_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789040.1"
                     /translation="MSPRYLGIDVGGTNSKLAVVEASDGDRPELLATVTLPTGAGDPG
                     EVIGRLAEAGARLVAGHGPVAAAGVGVPGLFDEASGRAVFLPNLPPAWAGREGLDAAT
                     VAAAARRAGRVD"
BASE COUNT          345 a         1095 c         1048 g          323 t
ORIGIN      
        1 cgccatgagc ggtcccatga accaggtccg gaccgtcctc ggcgacgtcc cctccgagcg
       61 gctcggcatg tgcttcgccc acgagcatct ggtcatcgac ggcggcgtgg ccaagctggt
      121 caacccggag atctccctgc agcgggttga tgacgcggtg gccgagctcc gcccgtgcgt
      181 ggccgccggg ctgcgggcgg tggtcgacgc catgccggcc gacgccggcc gcaacgtcgt
      241 caagctggcc gccatcagcc gccgggccgg ggtgcacgtc gtggccgcca ccggcctgca
      301 ccacgcccgc ttctacggcg agcggcactg gggcgagctg ctggcgcccg gggagctggc
      361 cgagctgttc gtggccgagc tggccgaggg catcgacgcc aacgacctca acgggccggt
      421 cgtccgccgc acgccgcacc gcgccggcgt ggtcaaggtg gccgggagcc ttggcgggcc
      481 cagcgaccgc gaccggcgcg ccttcgaggc cgcggccatg gccacggccc gggtcggggc
      541 cccgctgctg acccactgcg agcaagggac cggcggggtg gcccagctcc ggctcctgga
      601 ggagctcggc gtgccccttg accgggtgat cctgtcccac accgacaagg tggccgaccg
      661 cggctaccac cgcgagctcc tcaccagcgg cgcctacctg gtctacgacc agggcatccg
      721 caccccggag gccaccgccc gcctggtccg ctggatggtc tccgacggcc acggcgaccg
      781 gctcctgctc ggcaccgacg gcgcccggcg gtcgctgtgg tcggtgctcg gcggcacgcc
      841 cggcctggcc gccctccgca cccacctggg caagcgcctg gccgacgagc tcggccaggc
      901 cgccatggac cgcatctggg tcgccaaccc ggccgccgcc ctggccctgc ggccatgagc
      961 ggggcgggcc ggctggccgg gaaggtgtgc gtcgtcaccg gggccagcgg catggccgcc
     1021 gacgcggccc gccggttcgc ccgcgagggc gcccaggtct gggtggtggc ccgcgaccgc
     1081 gaccagtgcg aggccctcgg cctgccctac gccgtcgccg acctgcgcga cgagggcgag
     1141 accggggccg ccttcgcggc cctgcggcgt gcccatcccc gggtcgacgc cctctacgcc
     1201 gtggccgggg ccagcgggcg gccgctcggc gacgggccgg cccacgagct caccctggcc
     1261 gcctgggagg gcaccttggc gctcaacgcg accccggcgt tcctgtccgc ccgggaggcg
     1321 ctgcgggcca tgctcgacca gccgccgggc ccctcggcca cccgcggctc gctggtgctg
     1381 atgtcgagcg tcctggccgg ccacccgatg gcgccgctgt tcgccaccca cgcctacgcg
     1441 gccgccaagg cggccacgat cgggctggtc agggccatgg ccgcctacta cggcgagcat
     1501 gccatccggg tcaacgccct ggcgccttcg ctggtccgca cgccgatgag cgcccgggcg
     1561 gccgccgacc cggccagcgt ggccgccgcc gagcgccgcc agccgctgac cggcgggttc
     1621 ctggaggccg cggacgtgac cgaggccgcc gtctacctct gcagcgacga gagccgggcc
     1681 gtcaccgggc aggtgctggc ggtcgacggc ggctggaccg tgacctgagc cccgaaccaa
     1741 ccgacccgac cgaggagagg agacgccatg cccgaccgct ggaccgtcga gaccatcacc
     1801 gaggagaagg tcgccaagac gatcgaccac tcgctgctcc gccccgagct caccatcggg
     1861 gaggtgctgg ccggctgcga gctggccgcg gcctacgacg tggcctcggt ctgctgccgg
     1921 ccgctcgacg tcggccgctg ccgcgacgcc ctggcgggaa gcgccgtggc cgtcggcacg
     1981 gtcgtcggct tcccccacgg cagcagcacc acggccgtca aggcggccga ggcggcccgg
     2041 gcgctggacg acggggccac cgagctggac atggtcctgc ccatcggctg gctgcggtcg
     2101 ggggaggacg cctacgtcca cgacgacatc gccgccgtgg tggccgtggc ccgcccccgc
     2161 ggggccgtcg tcaaggtgat cctcgagaac gcctacctga ccgacgagga gaaggtgcgg
     2221 ggctgcgagc tgaccgagaa ggccggcgcc gactacgtga agacctcgac cggctacgcc
     2281 ccctccggct cgaccatgga cgacctccgc ctgatgcggg ccagcgtgtc cgagcgggtc
     2341 aaggtcaagg cggccggcgg catccgcacc ctcgacctgc tgatcgaggg cctcaacgcc
     2401 gggatggacc gctgcggggc cacggccacg gccgccatca tcgacgacct gcgagcgcgc
     2461 cagggctcgt gagcccccgc tacctcggca tcgacgtcgg ggggacgaac agcaagctcg
     2521 cggtcgtgga ggccagcgac ggcgaccggc ccgaactgct ggccacggtc accctcccga
     2581 ccggcgccgg cgaccccggc gaggtcatcg gccggctggc cgaggccggg gcgcggctgg
     2641 tggccgggca cgggccggtg gcggcggccg gggtgggcgt ccccgggctg ttcgacgagg
     2701 cgtcgggccg ggccgtgttc ctgcccaacc tcccgcccgc ctgggccggc cgcgaaggcc
     2761 tcgacgcagc cacggttgcc gcagccgcac ggcgggccgg ccgggtcgat g
//
LOCUS       DASSWR010000593         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_417712,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000593 DASSWR010000000
VERSION     DASSWR010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417712"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFO47_09865"
     CDS             complement(<1..262)
                     /locus_tag="VFO47_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HET8789041.1"
                     /translation="MTQAPDPGRVAGRRLDEVVAELAGTSRAQAARWASDGLVEVDGR
                     PRPKSYRLRGGERLAWTPPPAPPDGDPVAEDRPLTVRHEDDHL"
     gene            complement(259..780)
                     /gene="lspA"
                     /locus_tag="VFO47_09870"
     CDS             complement(259..780)
                     /gene="lspA"
                     /locus_tag="VFO47_09870"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET8789042.1"
                     /translation="MAEPAAAPRSRRMLALYGTAAAVLALDQLTKHLVVVNLAGRPPV
                     DVIGDLVQLRYTTNSGGAFSLLTGAPLFFGIMAMVIIGGIVYASRRAQPLSMLVVLGL
                     ILGGALGNLTDRLLRGEAFLRGEVVDFVKVGIWPVFNVADSCVVVGGILLALMLGRAE
                     REPEQAPADTERS"
     gene            complement(782..1228)
                     /locus_tag="VFO47_09875"
     CDS             complement(782..1228)
                     /locus_tag="VFO47_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET8789043.1"
                     /translation="MARKKTAEAAAGADDPVPRPRGRRSVLTKRDLQAYRRRLEAELA
                     ELQGQRADLEESTEASLADATGEVGFDEEFADTGSFTFERERDLSLVGNARDLIDKVE
                     HALARIDAGGFGRCEACGDAIEAERLDALPYATLCLADARQRARPR"
     gene            1570..>4151
                     /gene="ileS"
                     /locus_tag="VFO47_09880"
     CDS             1570..>4151
                     /gene="ileS"
                     /locus_tag="VFO47_09880"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET8789044.1"
                     /translation="MGQFPEVDPNLDLPALDARVLQLWRDRKVFERSVDQRVGAEPFV
                     FYEGPPTANGKPGVHHIEARVFKDLFPRFKTMRGHRVDRKGGWDCHGLPVELEIEKEL
                     GLKDKQAIERYGVEAFNARCRESVLRYVDVWEQLTERVGFWIDTGQAYRTMDASYVES
                     VWWALAELHRRGLLVEDYKVTPWCPRCQTTLSDAEVAMGYKEVEDLTAYVALPATTGT
                     LADEGAALLVWTTQPWTLVPNVSVVVGPEIDYVLVEATGEDGRARRLVVARDAVARAV
                     GEDARVLRPVPLEELLGTRYQRAFDLIPVTEEEAARGWTVVADEYVTTGDGSGLVQTS
                     PAYGAEDLAVGQRYGTPVLHPVGPDGKFGPETGWLAGRFVKAADADLVEDLRRRGLLW
                     RAETHSHAYPFCWRCGTPLIYYALTSWYARTTAKKDRLLAENARIGWRPEHIRDGRFG
                     DWLANNVDWALSRARYWGTPLPLWRCADGHVTAVESLADLSSRAGHDLSGLDPHRPFV
                     DEVAFACPDCGQEARRVPDVLDAWFDSGSMPFAQWGYPHSPEGRAAFQAQFPADFISE
                     AIDQTRGWFYSLLAVSTLLFDQTSYRNVVCLGHIVDREGRKMSKSLGNVLDPFELLDR
                     YGADPLRWFMLATGSPWVSRRLSPQAIEDITRSFFLTLWNTYSFFCLYARLEGFDPTR
                     PGSGAAAGRTPLDRWVLAELADTVEQVTQSLDDYDATGAARRLAAFVDDLSNWYVRLS
                     RRRFWRGTGADADAAFATLWTCLRTVTLLLAPYAPFTAEELWQGLVVSVDPESPDSVH
                     LVDWPEADRAALDPELSAAMAEVRRLVGLGRQARTEARIKVRQPLARALIGVPDRVRA
                     GVAGL"
BASE COUNT          540 a         1516 c         1546 g          549 t
ORIGIN      
        1 gcaggtggtc gtcctcgtgg cggacggtca ggggccggtc ctcggcgacc gggtcgccgt
       61 cgggtggggc cggcggcggg gtccaggcga gccgctcgcc gccccggagc cggtacgact
      121 tggggcgggg acggccgtcc acctcgacca ggccgtcgct ggcccagcgg gccgcctggg
      181 cccgggaggt gccggccagc tcggccacca cctcgtccag acggcgcccg gcgacccggc
      241 ccgggtcggg cgcctgggtc aagagcgctc ggtgtcggcc ggcgcctgct cgggctcgcg
      301 ctcggcccgg ccgagcatca gggcgagcag gatcccgccg acgaccacgc aggagtcggc
      361 cacgttgaac accggccaga tgccgacctt gacgaagtcg accacctcgc cccggaggaa
      421 ggcctcgccc cgcagcaggc ggtcggtcag gttccccagc gccccgccga ggatcaggcc
      481 gaggacgacc agcatggaca gcggctgggc gcggcgcgag gcgtagacga tcccgccgat
      541 gatgaccatg gccatgatcc cgaagaacag cggggccccg gtcagcagcg agaacgcccc
      601 accggagttg gtggtgtagc ggagctggac gaggtcgccg atcacgtcca ccggggggcg
      661 gccggccagg ttgacgacca ccaggtgctt ggtcagctgg tcgagggcga gcacggcggc
      721 ggcggtgccg tacagggcca gcatccgtcg ggaccgcggc gccgcggccg gctcggccat
      781 gtcagcgcgg ccgggcccgc tgacgggcgt cggccaggca cagggtggcg tagggcaggg
      841 cgtcgagccg ctcggcctcg atggcgtcgc cgcaggcctc gcagcgcccg aacccgccgg
      901 cgtcgatccg ggccagggcg tgctcgacct tgtcgatcag gtccctggcg ttgccgacca
      961 gcgacaggtc gcgctcgcgc tcgaaggtga agctgccggt gtcggcgaac tcctcgtcga
     1021 agccgacctc gccggtggcg tcggccaggc tggcctcggt cgactcctcc aggtcggccc
     1081 gctggccctg cagctcggcc agctcggcct ccagccgccg gcggtaggcc tgcaggtccc
     1141 gcttggtgag gaccgagcgg cggccgcgcg gtcgcggcac ggggtcgtcg gccccggcgg
     1201 cggcctcagc ggtcttcttg cgtgccatgg ctcctccgac caatccgatt gcctgtccgg
     1261 ggtagactga cgatacaggc aatgacggga acgagtaggg ggcggcacgg ccgccagcga
     1321 gccggggatg gtggaagccc ggcggcacgg cgcctctcga agatcctccc cgagccgctg
     1381 cccgaacggc cccagcccag taggcgcagc cgggtcagcc cgtgacagct ggagaccgtc
     1441 cggcggcgcc gggcggccgg tgagtggccg gtggccgtga ggccgccggc acgaggggtg
     1501 gtaccgcggg gccctcgcct cgtcccttcg ccaggtcgaa cacgacgacg cgaaggagac
     1561 gatcaccaga tgggccagtt ccccgaggtc gaccccaacc tcgacctccc cgcgctcgac
     1621 gcccgcgtgc tccagctgtg gcgcgaccgc aaggtgttcg agcgctcggt cgaccagcgg
     1681 gtgggggccg agccgttcgt cttctacgag gggccgccga ccgccaacgg caagcccggc
     1741 gtccaccaca tcgaggcccg ggtcttcaag gacctgttcc cccgcttcaa gaccatgcgc
     1801 ggccaccggg tcgaccgcaa gggcggctgg gactgccacg ggctgccggt cgagctggag
     1861 atcgagaagg agctggggct caaggacaag caggccatcg agcgctacgg ggtggaggcg
     1921 ttcaacgccc gctgccgcga gtcggtgctg cgctacgtcg acgtctggga gcagctcacc
     1981 gagcgggtcg gcttctggat cgacaccggc caggcctacc ggacgatgga cgcctcgtac
     2041 gtggagagcg tctggtgggc cctggccgag ctgcaccgcc gcggcctgct ggtcgaggac
     2101 tacaaggtca ccccctggtg cccccggtgc cagacgaccc tgtccgacgc cgaggtggcc
     2161 atgggctaca aggaggtcga ggacctcacc gcctacgtgg ccctgcccgc gaccaccggg
     2221 accctggccg acgagggggc ggccctgctc gtctggacga cccagccgtg gacgctggtc
     2281 cccaacgtct cggtggtggt cggccccgag atcgactacg tgctggtgga ggcgaccggg
     2341 gaggacggcc gggcgcgccg gctggtggtg gcccgggacg cggtcgccag ggcggtcggc
     2401 gaggacgcgc gggtgctgcg cccggtgccc ctggaggagc tgctcggcac ccgctaccag
     2461 cgggccttcg acctgatccc ggtgaccgag gaggaggcgg cccggggctg gacggtggtg
     2521 gccgacgagt acgtcaccac cggggacggg tcgggcctgg tccagacctc ccccgcctac
     2581 ggggccgagg acctggccgt gggccagcgc tacggcaccc cggtgctcca cccggtcggc
     2641 ccggacggga agttcgggcc ggagacgggc tggctggccg gccgcttcgt caaggccgcc
     2701 gacgccgacc tggtcgagga cctgcgccgg cgggggctgc tgtggcgggc cgagacccac
     2761 agccacgcct acccgttctg ctggcgctgc ggcaccccgc tgatctacta cgcgctgacc
     2821 tcctggtacg cccggaccac ggccaagaag gaccggctgc tggccgagaa cgcccgcatc
     2881 ggctggcgcc ccgagcacat ccgcgacggc cgcttcggcg actggctggc caacaacgtc
     2941 gactgggcgc tgtcgcgcgc ccgctactgg ggcaccccgc tgccgctgtg gcgctgtgcg
     3001 gacgggcacg tgacggccgt cgagagcctg gccgacctgt cgtccagggc cggccacgac
     3061 ctgtccgggc tcgacccgca ccggccgttc gtggacgagg tcgccttcgc ctgcccggac
     3121 tgcgggcagg aagcccgccg ggtccccgac gtgctcgacg cctggttcga ctccgggtcg
     3181 atgccgttcg cccagtgggg ctacccgcac agccccgagg gccgggccgc cttccaggcg
     3241 cagttcccgg ccgacttcat ctccgaggcc atcgaccaga cccgcgggtg gttctactcg
     3301 ctgctggccg tgagcacgct gctgttcgac cagacctcgt accgcaacgt ggtttgcctt
     3361 gggcacatcg tggaccgcga gggccgcaag atgtccaaga gccttggcaa cgtgctcgac
     3421 ccgttcgagc tccttgaccg ctacggggct gacccactgc gctggttcat gctggccacc
     3481 gggtcgccgt gggtgtcgcg gcggctgtcc ccccaggcga tcgaggacat cacccggtcg
     3541 ttcttcctca ccttgtggaa tacgtacagc ttcttctgcc tctacgcccg gctcgagggg
     3601 ttcgacccga cccgcccggg gtcgggggcg gccgccggcc gcaccccgct cgaccgctgg
     3661 gtcctggccg agctggccga caccgtggag caggtcaccc agagcctgga cgactacgac
     3721 gccaccggcg ccgcccggcg gctggccgcc ttcgtcgacg acctgtccaa ctggtacgtg
     3781 cggctgtcgc ggcggcgctt ctggcgcggc accggcgccg acgccgacgc cgccttcgcc
     3841 accctctgga cctgcctgcg cacggtgacg ctgctgctgg ccccgtacgc gccgttcacg
     3901 gccgaggagc tgtggcaggg gctggtcgtc tcggtcgacc ccgagtcccc ggactcggtc
     3961 cacctggtcg actggccgga ggccgaccgg gccgcgctcg accccgagct gagcgcggcc
     4021 atggccgagg tccggcggct ggtcggtctc ggccgccagg cccgcaccga ggccaggatc
     4081 aaggtccgcc agccgctcgc ccgggcgctg atcggcgtgc ccgaccgggt cagggccggc
     4141 gtggccgggc t
//
LOCUS       DASSWR010000594         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_304686,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000594 DASSWR010000000
VERSION     DASSWR010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304686"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFO47_09885"
     CDS             <1..264
                     /locus_tag="VFO47_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8789045.1"
                     /translation="DPSNQAHAKAAERLRAEPVAWLTTVRADGQAQSTPVWFLWDGDT
                     FLLYSQPDAQKVRNVTALGYEPGPFARIYSTPIRVRPTRVRVW"
     gene            269..1219
                     /locus_tag="VFO47_09890"
     CDS             269..1219
                     /locus_tag="VFO47_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8789046.1"
                     /translation="MAVAPEIQELLDRLAASGRPPLHRQSVAQARAFHVQDAPAQNGP
                     AVPVAAVADETVPGPGGELPVRVYTPEGTGPFPIVVWFHGGGWVVGTLDTYDPLCRAL
                     AASVPAVVVSVGYRLAPEHRWPAAAEDAYAATLWASRNAAALGGAQHRLAVAGDSAGG
                     NLAAAVALGARDRGGPAIAFQLLVYPVLDAAGDTASWREHAEGFYLTAAGMRWYWDHY
                     LGGADGAAPDASPLRAAFFGGLPPALVVGAEHDILRDEGEAYVARLRDAGVAATASRY
                     EGMVHGFFRWRAVTGAADAALQEAATALRTALAPPGAGPG"
     gene            complement(1216..2049)
                     /locus_tag="VFO47_09895"
     CDS             complement(1216..2049)
                     /locus_tag="VFO47_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET8789047.1"
                     /translation="MVSEPPPEPDYPRFRVEPGTRARLADIDPDESEGYKRKKDVADE
                     LKRHRDRISDLQARLYGEQKRGLLIVLQAMDTGGKDGTIKGVFQGVNPQGCQVWSFKA
                     PSNEEAAHDFLWRYHQKAPPKGMIHIFNRSHYEDVLIVRVKDLVPEPVWRPRYEAINQ
                     FEYALTADGVTVLKFFLHISKDEQKRRLESRLADPDKRWKFSANDLKERAWWDDYQAA
                     FEDAVNECSTAYAPWYVVPANNKWYRNLVVARTIADTLEAMSPKFPAAEKGLDKVKIP
                     D"
BASE COUNT          235 a          813 c          804 g          333 t
ORIGIN      
        1 gacccgtcca accaggccca cgccaaggcc gccgagcgcc tgcgcgccga gccggtcgcc
       61 tggctgacca ccgtccgggc cgacgggcag gcccagagca cgcccgtgtg gttcctgtgg
      121 gacggggaca cgttcctgct ctacagccag ccggacgccc agaaggtccg caacgtcacc
      181 gccctgggct acgagccggg cccgttcgcc cgcatctact cgacgcccat ccgggtccgc
      241 ccgacccggg tccgggtctg gtagcgccat ggccgtggcg ccggagatcc aggagctgct
      301 cgatcggctg gccgcctcgg gccggccgcc cctgcaccgc cagtcggtgg cccaggcgcg
      361 ggccttccac gtccaggacg ccccggccca gaacgggccg gccgtgccgg tggccgccgt
      421 ggccgacgag acggtgcccg ggccgggcgg ggagctgccg gtgcgggtct acacccccga
      481 gggcaccggg ccgttcccga tcgtggtctg gttccacggc ggcggctggg tggtcggcac
      541 cctcgacacc tacgacccgc tgtgccgggc cctggccgcc tcggtgccgg cggtggtggt
      601 gtcggtcggc taccggctgg cccccgagca ccgctggccg gccgcggccg aggacgccta
      661 tgcggccacc ctgtgggcgt cgcgcaacgc cgccgccctc ggcggcgccc agcaccggct
      721 ggccgtggcc ggcgacagcg ccggcggcaa cctggccgcg gccgtcgccc tgggggcccg
      781 cgaccggggc gggcccgcga tcgccttcca gctgctcgtc tacccggtgc tggacgcggc
      841 cggcgacacc gcctcctggc gcgagcacgc cgagggcttc tacctgaccg cggccgggat
      901 gcgctggtac tgggaccact acctgggcgg ggccgacggg gcggcgccgg acgcctcgcc
      961 gctgcgggcg gccttcttcg gcgggctgcc gccggccctg gtggtcgggg ccgagcacga
     1021 catcctccgc gacgagggcg aggcctacgt ggcccggctg cgcgacgccg gggtggccgc
     1081 caccgccagc cgctatgagg gcatggtcca cggcttcttc cgctggcggg ccgtgaccgg
     1141 cgccgccgac gccgccctcc aggaggccgc caccgccctc cggacggccc tggccccgcc
     1201 cggcgccggc cctggctagt cggggatctt gaccttgtcg aggcccttct cggccgccgg
     1261 gaacttgggg ctcatggcct ccagggtgtc ggcgatggtc cgggccacga ccaggttgcg
     1321 gtaccacttg ttgttggccg ggaccacgta ccagggggcg taggcggtcg agcactcgtt
     1381 gaccgcgtcc tcgaacgccg cctggtagtc gtcccaccag gcgcgttcct tgaggtcgtt
     1441 ggccgagaac ttccagcgct tgtccgggtc ggccaggcgg ctctccaggc ggcgcttctg
     1501 ctcgtccttg gagatgtgga ggaagaactt gagcaccgtg accccgtcgg cggtgagggc
     1561 gtactcgaac tggttgatgg cctcgtacct gggccgccag accggctcgg ggacgaggtc
     1621 cttgacccgg acgatgagca cgtcctcgta gtgggagcgg ttgaagatgt ggatcatgcc
     1681 cttgggcggc gccttctggt ggtagcgcca gaggaagtcg tgggccgcct cctcgttcga
     1741 gggcgccttg aacgaccaca cctggcagcc ctgcgggttg acgccctgga acacgccctt
     1801 gatggtgccg tccttgccgc cggtgtccat cgcctggagc acgatcagca ggccgcgctt
     1861 ctgctcgccg tagagcctgg cctggaggtc gctgatccgg tcgcggtgcc gcttgagctc
     1921 gtcggccacg tccttcttgc gcttgtagcc ttcggactcg tcggggtcga tgtcggccag
     1981 ccgcgcccgc gtccccggct cgacccggaa ccgcgggtag tccggctccg gcggcggctc
     2041 ggacaccacc gaccgctcgg gacgggtgac agtgctggcc cggtcgacca cctcggccgt
     2101 ggccgtggcg gccttggccg acgctccctt gtcccgggtc gcgctgctgt gggccgtgct
     2161 gctctggctg tccctcctgg tcgtg
//
LOCUS       DASSWR010000595         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_50534,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000595 DASSWR010000000
VERSION     DASSWR010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50534"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFO47_09900"
     CDS             complement(<1..340)
                     /locus_tag="VFO47_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET8789048.1"
                     /translation="MSAEWPGLLWMLLLVPAALAAYLLAQRRRSRYTVRYTNLDLLAN
                     VVSAKPGWRRHLPPALYLLALAALVVSMARPQALTLVPKEQATIVLVMDVSGSMNATD
                     VQPTRLVSSQR"
     gene            complement(357..1523)
                     /locus_tag="VFO47_09905"
     CDS             complement(357..1523)
                     /locus_tag="VFO47_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8789049.1"
                     /translation="MNDPLGIERTPDRPGPGPTPEALLRALDVVVRRRIEAMLTGDHR
                     SSTIGIGTELAQIRLYQPGDDVRRIDWNVTARTGDIHVRDQIAERALVSWLVLDTSPS
                     MTFGTADRRKADVAEGVALAVGHVGTRHGNRLGLITFGDSRPVTIPSRQGRAGLLGML
                     ATVRREPQAEGGGATSLGEALARTGRLATSRSLIFVVSDFRGPRDWRRELIELAGRHD
                     VVAVEIRDPREQELPDVGELWLVDPETGRQLRVDTSRRRLRERFAAAAANERADLAGL
                     LQSSGADHVVLWTAGDWLRSFAGFLRHRVTGPGSARGPAPVQWGPEAAHGGGSADVVA
                     HPPQAVGAPVGGGAPPPRPWSPFPGGGPPGPGGRPGPGWSPGPGGPPGPGRPPR"
     gene            complement(1529..2521)
                     /locus_tag="VFO47_09910"
                     /pseudo
     CDS             complement(1529..2521)
                     /locus_tag="VFO47_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515971.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          354 a          888 c          971 g          339 t
ORIGIN      
        1 cccgctggga cgacaccagc cgggtcggct ggacgtcggt ggcgttcatc gaccccgaca
       61 cgtccatcac caggacgatg gtcgcctgct ccttgggcac cagggtcagc gcctggggcc
      121 gggccatgct gacgaccagg gcggcgaggg cgagcaggta gagcgccggg gggaggtggc
      181 ggcgccatcc cggcttggcg ctgaccacgt tggccagcag gtcgaggttg gtgtagcgga
      241 cggtgtaacg ggagcggcgt cgctgggcga gcaggtaggc ggcgagcgcg gccgggacga
      301 gcagcagcat ccagagcagg cccggccact ccgcgctcac cggacgccgc cccgtttcac
      361 ctcggtggcc tcccggggcc gggcgggccc ccgggtcccg gcgaccaccc ggggccgggc
      421 cggcctccgg ggccgggtgg gcctccgccg gggaaggggg accaggggcg cggcggcggg
      481 gcgccgcccc cgacgggcgc tcccacggcc tgcggcgggt gggcgacgac gtcggccgag
      541 ccgccgccgt gggcggcctc cggaccccac tggaccgggg ccgggccgcg ggcgctgccc
      601 gggccggtga cgcggtggcg caggaacccg gcgaagctgc gcagccagtc cccggccgtc
      661 cagagcacga cgtggtcggc gccggacgac tgcagcaggc cggccaggtc ggcccgctcg
      721 ttcgcggcgg cggccgcgaa ccgctcccgc agccggcgcc ggctcgtgtc gacccggagc
      781 tggcggcccg tctcggggtc gaccagccac agctcgccca cgtcgggcag ctcctgctca
      841 cgcgggtcgc ggatctcgac cgcgaccacg tcgtgccggc cggccagctc gatcagctcc
      901 cggcgccagt cgcgggggcc gcggaagtcg ctgaccacga agatcaggga ccggctggtg
      961 gccagccgcc cggtccgggc cagggcctcg cccagcgagg tggccccgcc accctcggcc
     1021 tgcggctcgc ggcggaccgt ggccagcatc cccagcagcc cggcccggcc ctggcgcgac
     1081 gggatcgtca ccggccggct gtcgccgaag gtgatcagcc ccaggcggtt gccgtgccgg
     1141 gtcccgacgt ggccgacggc cagggcgacc ccctcggcca cgtcggcctt gcgccggtcg
     1201 gccgtgccga aggtcatcga cggcgaggtg tccaggacca gccagctgac cagggcccgc
     1261 tcggcgatct ggtcacgcac gtggatgtcg ccggtccggg cggtgacgtt ccagtcgatg
     1321 cggcggacgt cgtcgcccgg ctggtacagc cggatctggg ccagctcggt cccgatgccg
     1381 atcgtgctcg agcggtggtc tccggtgagc atggcctcga tccggcggcg cacgaccacg
     1441 tcgagggcgc gcagcagcgc ctccggggtc ggtccgggac ctggccggtc gggggtgcgt
     1501 tcgatgccga ggggatcgtt catgctggtt gttctctggc gaggtcgatg cggggtgggg
     1561 ggatggcggc caggaccgcg gtcaacaagc tgtccgggct gacctgctcg gccaatgcct
     1621 gataggtcaa gaccaaccga tggcgcagga catcgcgggc cagttcctgg acatcctggg
     1681 gcaggcagta gctgcggccc cggagcacgg ccaaggcccg ggcggccgcg accaggttga
     1741 tcgagccccg cgggctcgcc ccataggcga tatagccgcc aagatcagcc aggttgtagg
     1801 tcgctggctg gcgggtcgcg tcacacaacg ccaccgcgta ctcggccacc agccgatcca
     1861 catacaccgt ggccgtgtgg gcctggaggg cctggagctg ctccagggtc aagatcttgg
     1921 cgatctcggg gggctcggtc aggctccgag ccacgatcgt catctcttcg gagaagtttg
     1981 gatagtcgac cacgaccttc atcaagaacc ggtcgacctg ggcctcgggc aacgggtagg
     2041 tgccgtcgga ctcgatcggg ttctgggtgg ccaggaccag gaacggggcc ggcacggcat
     2101 aggactgttt gccgatggtc acctggtgtt cctgcatcac ctccaacaac gccgactgga
     2161 ccttggccgg ggcccggttg atctcatcgg ccaacaaaaa gttgcagaac accgggccca
     2221 gttcggtttc gaagccgccc tggtcgggcc gccagatgcg ggtgccgacc aggtcgctgg
     2281 ggaccaggtc gggggtgaac tggatgcgct ggaagctccc gcccaggact tgggccaggg
     2341 tggcgatggt gcgggtcttg gccagcccgg gcaccccttc cagcagcaga tggccaccgg
     2401 acaacaacgc caccaggacc cgctccagca tgccctcctg gccgacaatg acccgcttga
     2461 tctcaaacaa cgccgcctcg agctgctggc gggcgacggg ggcctggcct acggaggcca
     2521 ggcgggaggg ttggtcggtc acctggcgag ct
//
LOCUS       DASSWR010000596         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_164097,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000596 DASSWR010000000
VERSION     DASSWR010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164097"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFO47_09915"
     CDS             <1..494
                     /locus_tag="VFO47_09915"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HET8789050.1"
                     /translation="LSGTVLTPEHGTPLDVRYRVEAGPDGLTRRVELELDGGAVRRVL
                     LADGAGRWRWGDGPELPEVAGALDVDLTVTPATNTLPIRRLARHLETGQAADLQMAWV
                     QFPDLEVILSAQRYERLAPDRWRFSTGDFSAELRVDPDGLVLDYGGLFRSLAYRPDTR
                     EIR"
     gene            complement(487..1887)
                     /locus_tag="VFO47_09920"
     CDS             complement(487..1887)
                     /locus_tag="VFO47_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HET8789051.1"
                     /translation="MAQTTNPGPFFSRVPRAHAASQPFTYPLQRDWAEPDWTRLPGFK
                     NVTRDEWETAQWQRAHTVKNLRQLLAVYGDLFPADLLASVERDQAERATMSMLLPPQM
                     LNTMDERDLWADPVRRYMLPAFDDRDPEWPSHPLSTRDSLHEADMWAVEGLTHRYPTK
                     VLAELLPTCPQYCGHCTRMDLVGNSTPQVAKYKFEIKQPARHEQILDYLRRTPSVRDV
                     VVSGGDVANLPIRVLEQFVSALLDIDNIRDIRLASKGLMGLPQHFLQDDVLAGLARLA
                     TKANDRGVDLALHTHVNHANQLTPLVAKAAGALHDMGFRDVRNQGVLLRGVNATPKDL
                     LDLCFTLLDQAKVMPYYFYVCDMIPNSEHWRVSIAEAQELQHAIMGYLPGFATPRVVA
                     DIPFVGKRWVHQVEAYDREKGISYWTKNYRTGIEFDDPDALSRRYEYYDPIRTLPESG
                     KEYWRRFGVPDTVPVT"
BASE COUNT          291 a          747 c          827 g          333 t
ORIGIN      
        1 ggctgtcggg gaccgtgctc acccccgagc acggcactcc actggacgtc cgctaccggg
       61 tcgaggccgg cccggacggc ctgacccgcc gggtcgagct ggagctggac ggcggggcgg
      121 tccgccgggt cctgctggcc gacggcgccg gccgctggcg ctggggggac ggccccgagc
      181 tgcccgaggt cgccggcgcc ctcgacgtcg acctcaccgt caccccggcc accaacaccc
      241 tccccatccg ccgcctggct cgccatctcg agaccggcca ggcggccgac ctccagatgg
      301 cctgggtcca gttccccgac ctcgaggtca ttctctcggc ccagcgctat gagcgcctgg
      361 cccccgaccg ctggcggttc tccaccggcg acttctccgc cgagctccgg gtcgacccgg
      421 acggcctcgt cctcgactac ggcggcctgt tccgctccct ggcctaccgc cccgacacca
      481 gggagatcag gtgaccggca cggtgtccgg gacgccgaac ctgcgccagt actccttgcc
      541 ggactcaggg agggtccgga tcgggtcgta gtactcgtag cggcggctga gggcgtcggg
      601 gtcgtcgaac tcgatgccgg tgcggtagtt cttggtccag taggagatgc ccttctcgcg
      661 gtcgtaggcc tcgacctggt ggacccagcg cttgccgacg aaggggatgt cggccaccac
      721 ccggggggtg gcgaagccgg gcaggtagcc catgatcgcg tgctgcagct cctgggcctc
      781 ggcgatcgag acccgccagt gctcggagtt ggggatcatg tcgcagacgt agaagtagta
      841 cggcatgacc ttggcctggt cgagcagggt gaagcagagg tcgagcaggt ccttgggggt
      901 ggcgttgacc cctcgcagca gcactccctg gttgcggacg tcacggaagc ccatgtcgtg
      961 cagggccccg gcggccttgg cgaccagcgg ggtgagctgg ttggcgtggt tgacgtgggt
     1021 gtgcagggcc aggtcgacgc cgcggtcgtt ggccttggtg gccaggcggg ccaggccggc
     1081 caggacgtcg tcctggagga agtgctgggg caggcccatc agccccttgg aggccagccg
     1141 gatgtcacgg atgttgtcga tgtcgagcag ggccgacacg aactgctcca ggacccggat
     1201 gggcaggttg gccacgtcgc cgccggacac gaccacgtcg cgcacgctcg gggtgcggcg
     1261 caggtagtcg aggatctgct cgtgacgggc cggctgcttg atctcgaact tgtacttggc
     1321 cacctgcggg gtcgagttgc cgaccaggtc catgcgggtg cagtggccgc agtactgggg
     1381 gcaggtgggc agcagctcgg ccaggacctt ggtggggtag cggtgggtca ggccctccac
     1441 cgcccacatg tcggcctcgt gcaggctgtc gcgggtcgac agcgggtggc tgggccattc
     1501 cgggtcgcgg tcgtcgaagg ccgggagcat gtagcggcgc accgggtcgg cccacaggtc
     1561 ccgctcgtcc atggtgttga gcatctgggg cgggagcagc atcgacatgg tggcccgctc
     1621 ggcctggtcg cgctcgaccg aggcgagcag gtcggccggg aacaggtcgc cgtacaccgc
     1681 cagcagctgg cggaggttct tgacggtgtg ggcccgctgc cactgggccg tctcccactc
     1741 gtcccgggtc acgttcttga agccggggag ccgggtccag tccggctcgg cccagtcgcg
     1801 ctggaggggg taggtgaacg gctgggaggc cgcgtgggcc ctcgggacac gggagaagaa
     1861 gggcccggga ttggtggtct gcgccatcgg tggtgccttt gcgtccgcag gtgcctgctc
     1921 ggcctaccgg cagggtcctt cgcaacctgt ggcccgctgt ggggcgcggc gccacgatag
     1981 cagaccccca ggtgcgcaga aacacccgct tgtactacct gcctacgacc cgccgggcca
     2041 gtgggcggcc gcctcggcca gcgacccgta gcgcccctcc agccgccagc cggccatcac
     2101 cgccggggcg acgaagcggg ttcgcggcgg ggccagcggc cggccggccc gcagcccgag
     2161 cacactggcc cggaacagcg cccggcgggc aggcccgc
//
LOCUS       DASSWR010000597         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1039570, whole genome shotgun sequence.
ACCESSION   DASSWR010000597 DASSWR010000000
VERSION     DASSWR010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039570"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFO47_09925"
     CDS             <1..270
                     /locus_tag="VFO47_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8789052.1"
                     /translation="APDVILLDLSMPVLNGLDVTRLLREVAPATAVVIFSAFDSPELK
                     RQAFEAGAVAYLSKGCSNERLRATLEAAVTVAGSNLRRAGTALGR"
     gene            360..983
                     /locus_tag="VFO47_09930"
     CDS             360..983
                     /locus_tag="VFO47_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET8789053.1"
                     /translation="MSNLVPVVIEQTSRGERSFDLYSRLLSGRIVFLGTPIDDTSANL
                     IMAQLIHLESEDPDKDIQLYINSPGGSVTALLGIYDTMRYIRCDVATTCLGWAASAAA
                     VLLAAGTPGKRYALPHSTVLLHQPHGGAQGQSVDIDIQAREILRQRVLVDRILAEHTG
                     QDVEKIARDTDRDFILDAGQALEYGLVDEILTGRRSVPELEPAGARG"
     gene            988..1299
                     /locus_tag="VFO47_09935"
     CDS             988..1299
                     /locus_tag="VFO47_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789054.1"
                     /translation="MLGPRRPAPDRPAMPALTDAECSFVDHYLAVVDLTARINPARSS
                     GHAYACLRAAQSLATEARALLAALELMWERGEREIHVPTLTQALRALDGERRASRVTI
                     P"
     gene            1414..2472
                     /locus_tag="VFO47_09940"
     CDS             1414..2472
                     /locus_tag="VFO47_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789055.1"
                     /translation="MLAWTVWVLTVLGLAGTLLIDRLLHQAGRADLTALTASAVPLAV
                     AAISAVTVGAVVAARRPRHPVGWLLLAMGLSITVHELTYSYTRYGLVVRPGAVPGAAY
                     LAGLNNGVVVQWIACAGFVLLLTPTGRLPSPRWRWWARIAAAAAVLWLLGSIVDPVPL
                     RPEYPDIANPLGVPALSGALSLLMFAALVVLVSLIVGAVALLLRYRRARGVERLQLRW
                     LIWGAAVASLALVAAIASLILNDDFTVLNFALGVSAAMFPLATGAAILRYRLYDLDQI
                     ISRTVAYALLTILLGLGYAAVVLGLGRLLPDSSSLGVAAATLAVAAVFQPARRRVQHA
                     VDRRFNRRRSDAARTIEA"
BASE COUNT          337 a         1087 c          947 g          383 t
ORIGIN      
        1 gcccccgacg tgatcctgtt ggacctgtcc atgcccgtcc tcaacggcct ggacgtgacc
       61 cggctgctgc gcgaggtggc gccggccacg gccgtcgtga tcttcagcgc cttcgacagc
      121 cccgagctca agcgccaggc cttcgaggcc ggggcggtcg cctacctctc caagggctgc
      181 agcaacgagc gcctgcgggc caccctggag gcggccgtca ccgtcgccgg cagcaacctc
      241 cgccgggccg gcacggcgct cggccggtag cggaatccgg ccccggcagg ccggcgctgt
      301 ggcacggttg gccgcgaagg accggcgccg ccggtgccgc gcatgtcgct ggaggccaca
      361 tgtcgaacct cgtgcccgtc gtcatcgagc agaccagccg gggcgagcgg tcgttcgacc
      421 tctactcgag gctgctgagc ggccggatcg tcttcctcgg gaccccgatc gacgacacca
      481 gcgccaacct gatcatggcc cagctcatcc acctggagag cgaggacccc gacaaggaca
      541 tccagctcta catcaactcc cccggcgggt cggtgaccgc cctgctcggc atctacgaca
      601 ccatgcgcta catccgctgc gacgtcgcca ccacctgcct gggctgggcc gcgtcggcgg
      661 ccgcggtgct gctggccgcc gggacgcccg gcaagcgcta cgccctcccc cactccaccg
      721 tcctgctcca ccagccccac ggcggcgccc agggccagtc ggtcgacatc gacatccagg
      781 cccgcgagat cctgcgccag cgggtcctgg tcgaccggat cctggccgag cacaccggcc
      841 aggacgtgga gaagatcgcc cgcgacaccg accgcgactt catcctcgac gccggccagg
      901 ccctggagta cgggctggtc gacgagatcc tcaccggccg ccggagcgtc cccgagctcg
      961 aaccggccgg cgcccgcggg tgaccggatg ctcggccccc ggcgccccgc ccccgaccgg
     1021 ccggcgatgc cggccctgac cgacgccgaa tgctcgttcg tcgaccacta cctggccgtg
     1081 gtcgacttga cggcccgcat caacccggcc cgcagcagcg gccacgccta cgcctgcctg
     1141 cgggccgccc agtccctggc caccgaggcc agggccctgc tggccgcgct cgagctgatg
     1201 tgggagcggg gcgaacgcga gatccacgtc cccaccctca cccaggccct ccgcgccctc
     1261 gacggcgagc gccgcgccag cagggtcacc atcccgtaac gctcccggga cggaagcccg
     1321 gcacactgcc actatcccgc gcggagatcg agacgggagt cggtgcggat ggatgctgcg
     1381 accgggtcgg cggggccgcg aaggtggccg gccatgctgg cctggacggt ctgggtgctg
     1441 accgtgctcg ggctggccgg gacccttctg atcgaccgcc tgcttcacca ggccggacgg
     1501 gccgacctga cggcgctgac ggccagcgcc gtcccgctgg ccgtggcggc catcagcgcc
     1561 gtgaccgtcg gggcggtggt ggccgcccgc cggccccgcc acccggttgg ctggctgctg
     1621 ctcgccatgg ggctgtccat caccgtccac gagctcacct acagctacac ccgctacggg
     1681 ctggtcgtcc ggcccggcgc cgtccccggc gccgcctacc tggccggact caacaacggc
     1741 gtggtcgtgc agtggatcgc ctgcgccggc ttcgtgctcc tgctcacccc gaccgggcgg
     1801 ctgccgtcgc cccgctggcg ctggtgggcc aggatcgcgg ccgccgcggc cgtgctgtgg
     1861 ctgctgggct cgatcgtcga cccggttccg ctgcgccccg agtaccccga catcgccaac
     1921 ccgttgggcg tcccggccct gtccggagcg ctgagcctgc tcatgttcgc cgccctggtc
     1981 gtcctcgtct cgctgatcgt cggggcggtg gcgttgctgc tgcggtaccg ccgcgcccgc
     2041 ggggtcgagc ggctgcagct gcgctggctg atctgggggg cggcggtggc ctccctggcg
     2101 ctggtggccg ccatcgcctc actgatcctc aacgacgact tcaccgtgct gaacttcgcc
     2161 ctcggcgtca gcgcggccat gttcccgctg gccaccggcg cggccatcct gcgctaccgg
     2221 ctgtacgacc tggaccagat catcagccgc acggtggcct acgcgctgct cacgatcctg
     2281 ctcggcctcg ggtacgcggc cgtggtcctg ggcctcggcc gcctcctgcc cgacagctcc
     2341 agcctgggcg tggccgccgc caccctggcc gtggccgcgg tgttccagcc ggcccgccgc
     2401 cgggttcagc acgccgtcga ccgccgcttc aaccgccgcc gctccgacgc cgcccggacc
     2461 atcgaggcgt agccgaggcc gcctccggaa cgaggtcgac ctggacaccc tgacggccga
     2521 gctgctggcc gtgaccgacc agacggtgca gcccaccgcc gcatcgctgt ggctgcgccc
     2581 ggcccctagg cgctgagggc ggcggccagg aaccggagct gggccggggc ctgctcctgc
     2641 cagtagcggc cgtcgtggca gcccttggtc acggcgatct gggcgacggg gagggtggtg
     2701 gccagggcgc gagcgccggg ggcgaacggg tcgttggcac cgcagtcgac cttg
//
LOCUS       DASSWR010000598         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_954399,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000598 DASSWR010000000
VERSION     DASSWR010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954399"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="rsgA"
                     /locus_tag="VFO47_09945"
     CDS             <1..938
                     /gene="rsgA"
                     /locus_tag="VFO47_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET8789056.1"
                     /translation="ELLTRPTGRLRHLAGPGVAGLPAVGDWVAVRDGRTSGVLPRRTA
                     VTRRAPGSGAGEQVLAANVDLVLVVVAPGRDANPRRVERLLALAWESGARPVVVLGRA
                     DLCPDWGTDVATELAALAAVAPGVQVLALSCYTGEGVDEIAAMLDPGRTAVLLGSSGV
                     GKSTLVNRLAGRDLLATGELRDDGKGRHTTTTRQLVPLAGGGLIIDTPGLRELGLWTG
                     ASGTAAAFDDIETLAAECRFDDCRHRTEPGCAVLQALEDGRLPAERFTAWEKLQRELA
                     WAERRADPLAAANRRRQIRALSRSVRAGYLQRGRD"
     gene            complement(948..>2036)
                     /locus_tag="VFO47_09950"
     CDS             complement(948..>2036)
                     /locus_tag="VFO47_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET8789057.1"
                     /translation="VVLFGATGYTGRLTAEALAAAGDRPVLAGRDRGRLDALAGRLGD
                     LPVAGADTARPDSVRDLVGPGDVLVSTVGPFLTRGEPAVRAAVDAGAAYLDATGEPPF
                     LRRVFEEHGPRAAGRCGLVPAFGYDFVPGNLAAALALAEAGAGRATRVAVGYFVTGRG
                     GFSSGTMASGAGLLLEPGFAWRDGRLRRERMARRVRAFPIGGREWLGVSYGGSEHFAL
                     PRIAPGLREVEVYLGWFGRRSRAVRAASALGAPLTRLPGTRSLAQAAAGRLARRTGQG
                     PDEAARRRCGSLIVALASDAEGRELAQVRLRGPDPYTLTARLLAWGARRAAGHGLAAT
                     GALGPVDAFGLEALQAGAAEAGLERDAP"
BASE COUNT          222 a          821 c          802 g          191 t
ORIGIN      
        1 gcgagctgct cacccggccc accggccgcc tgcgccatct ggccggcccc ggcgtggccg
       61 ggctgccggc ggtcggcgac tgggtggcgg tccgcgacgg gcgcacctcc ggggtgctcc
      121 cccgccgcac ggcggtgacc cgccgggcgc ccggatcggg ggccggcgag caggtcctgg
      181 ccgccaacgt cgacctggtg ctggtggtgg tggccccggg acgtgacgcc aacccgcgcc
      241 gggtggagcg gctgctcgcc ctggcctggg agagcggcgc ccggccggtg gtcgtgctcg
      301 gccgggccga cctgtgcccc gactggggca ccgacgtggc caccgagctg gccgccctgg
      361 ccgcggtcgc cccgggcgtc caggtgctgg ccctgagctg ctacaccggc gagggagtgg
      421 acgagatcgc ggccatgctg gaccctggcc gcaccgcggt cctgctcggc tcctccggcg
      481 tcggcaagtc gaccctggtc aaccgtctgg ccggccggga cctgctggcc accggcgagc
      541 tcagggacga cggcaagggc cgccacacga ccaccacccg ccagctggtc ccgctggccg
      601 gcggtggcct gatcatcgac acccccggcc tgcgcgagct cggtctgtgg accggcgcct
      661 cggggaccgc ggccgccttc gacgacatcg agaccctggc cgccgaatgc cgcttcgacg
      721 actgccgcca ccggaccgag cccggctgcg cggtcctcca ggcgctggag gacggccggc
      781 tgccggcgga gcggttcacc gcctgggaga agctgcagcg cgagctggcc tgggccgagc
      841 ggcgcgccga ccccctggcc gccgccaacc ggcgccgcca gatccgcgcc ctgagcaggt
      901 cggtccgggc cggctacctg cagcgcgggc gggactgacc gacccggtca gggggcgtcg
      961 cgctccaggc cggcctcggc cgcgccggcc tggagggcct cgaggccgaa ggcgtcgacc
     1021 gggccgaggg cgccggtggc ggccaggccg tggccggccg cccggcgggc gccccaggcg
     1081 agcagccggg ccgtgagggt gtaggggtcg gggccgcgca ggcggacctg ggccagctcg
     1141 cggccctcgg cgtcgctggc cagggccacg atcagggagc cgcagcgccg gcgggccgcc
     1201 tcgtccgggc cctggccggt gcggcgggcc agccggccgg cggcggcctg ggccagggac
     1261 cgggttccgg gcaggcgggt cagcggcgcc cccagcgccg aggcggcccg cacggcccgg
     1321 gagcggcggc cgaaccagcc caggtacacc tcgacctcgc gcaggccggg cgcgatccgg
     1381 ggcagggcga agtgctcgct gccgccgtag gacacgccca gccactcgcg gcccccgatc
     1441 gggaacgccc gcacccgccg ggccatccgc tcccgccgca gccgcccgtc ccgccaggcg
     1501 aaccccggct ccagcagcag cccggccccc gaggccatgg tcccgctgct gaacccgccc
     1561 cggccggtca cgaagtagcc gacggcgacc cgggtcgccc gccccgcgcc cgcctcggcc
     1621 agagcgagag cggcggccag gttgccgggg acgaagtcgt agccgaaggc cgggaccagg
     1681 ccgcagcggc cggccgccct cgggccgtgc tcctcgaaca cgcggcgcag gaacggcggc
     1741 tccccggtgg cgtccaggta ggccgcgccg gcgtcgacgg ccgccctgac cgctggctcg
     1801 ccccgggtca ggaaggggcc gacggtgctg acgagcacgt caccggggcc gacgaggtcg
     1861 cggacgctgt ccggccgggc cgtgtcggcc ccggccaccg gcaggtcccc gagccggccg
     1921 gccagggcgt ctagccggcc ccggtcgcgg ccggccagca ccggccggtc gcccgccgcg
     1981 gccagggcct cggccgtcag ccggcccgtg tacccggtgg ccccgaacag caccac
//
LOCUS       DASSWR010000599         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1039617, whole genome shotgun sequence.
ACCESSION   DASSWR010000599 DASSWR010000000
VERSION     DASSWR010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039617"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFO47_09955"
     CDS             <1..710
                     /locus_tag="VFO47_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8789058.1"
                     /translation="TGRPPLVPPTLPVLVLSGGLDTLTTWTDGAMVAEQLGPSARWVK
                     VENTIHVTALADPYGCASGLVREFIRHPERLHAMDVSCASRIPEVRVVGEFPRRLSGA
                     TPATPGKGNDAGATGLRLAAVGAAAVGDAIAQWWYLPGSRGHGLRGGWFTVEGDTSVK
                     FDLHKVRFVADTVVDGQATWNTGSGQVRARVVVKGPGGATATLRMRWDDLARHPRATV
                     TGHIGSGTRLAATLPAP"
     gene            1007..1714
                     /locus_tag="VFO47_09960"
     CDS             1007..1714
                     /locus_tag="VFO47_09960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8789059.1"
                     /translation="MELYALLPPMGEPEVVHAAIPAGASILELGAGAGRMTHRLVELG
                     HPVVAVDESPEMLERIHGAAPGTPAEARPTGAAEAGVETVQARIQDLDLNRRFDAVLL
                     ASFLVNTPDDDLRHRFLAACRNHLGDGGCVLVQRHRPGWFDEATEEERTSGGVTFRLR
                     DLRRPGPGLLAATAEYRVGERVWTHAFTTRRLDDGELAAALAEAGLAVDAYLTGDGSW
                     VRAVPASRGDALGRWPR"
     gene            complement(1636..2847)
                     /locus_tag="VFO47_09965"
     CDS             complement(1636..2847)
                     /locus_tag="VFO47_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET8789060.1"
                     /translation="MLDATAPPFDPADLEPTLRPFGQSRMLPRDAYVSPRVFAFEQER
                     FFAGSWTCVGREGDLAGTGAQRAVKVGGAGVLLARGTDGRMRAFANTCRHRGHELLGV
                     GERTNRRTVLCPYHAWTYDLDGALRAAPGFRDHGDFRPAEHGLVELPLESWHGFLFVN
                     GSGDAPPFAEHVGALDQLVAPYRPERLVPLATHEYDLACNWKVILENYHECYHCPLIH
                     PELCQVSPPASGDNFELDGAWVGGTMDLKDHAVTMSLDGHSDGVPMPGLDDERLRTVA
                     YLGLFPNLLLSLHPDYLMTHLVEPLGPDLSHVACTWYFPPEAAERPGFDPAYAVDFWD
                     RTNRQDWAACESVQRGMASPHFQPGPLAPAEDAVYHVVTMIARAYLGQRPSASPRDAG
                     TALTQEPSPVR"
     gene            2940..3554
                     /locus_tag="VFO47_09970"
     CDS             2940..3554
                     /locus_tag="VFO47_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005308850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET8789061.1"
                     /translation="MARIKADVRREEILKATCREVIARGFASTRVGDVAAALGVSTGL
                     VFYHFSKKETLLGEAFRHAAGEDLDRLSATAAGDGSAAQRLDRILRLYSPAGSSEAWL
                     LWIDAWGQALRSPELAEVSRQLDLRWKQTLAAVIREGVAAGEFTCPDPDAAAWRLTAL
                     LDGLGVQVTVHGSVPDARLVDWVRAAAAAELAVDPSVLAGDGRP"
     gene            3621..>3902
                     /locus_tag="VFO47_09975"
     CDS             3621..>3902
                     /locus_tag="VFO47_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8789062.1"
                     /translation="MAVGVQVTFDAADPQALAAFWGETLGYVEQDPPAGFDSWEAWAV
                     ANDVPREKWGDYDARVDPDGAGPRLYFQRVPEPKTAKNRVHLDLEVSGRG"
BASE COUNT          512 a         1394 c         1516 g          480 t
ORIGIN      
        1 tcaccggacg gccgccgctg gtcccgccga ccctgccggt gctggtgctg tcgggcgggc
       61 tcgacaccct gaccacctgg accgacggcg ccatggtggc cgagcagctg ggcccgtcgg
      121 cccgctgggt caaggtcgag aacaccatcc acgtgaccgc gctggccgac ccctacggct
      181 gcgccagcgg gctcgtccgc gagttcatcc gccacccgga gcggctgcac gccatggacg
      241 tgtcctgcgc cagccgcatc cccgaggtcc gcgtggtcgg cgagttcccg cggcggctgt
      301 ccggggccac cccggcgaca cccggcaagg gcaacgacgc cggggcgacc gggctgcggc
      361 tggccgccgt cggggcggcc gcggtcggcg acgccatcgc ccagtggtgg tacctgcccg
      421 ggtcgcgcgg ccacgggctc cgcggcggct ggttcaccgt cgagggggac accagcgtca
      481 agttcgacct gcacaaggtc cgcttcgtcg ccgacacggt cgtcgacggc caggccacct
      541 ggaacaccgg cagcggccag gtccgcgccc gggtcgtcgt caagggcccg ggcggcgcca
      601 ccgccaccct ccgcatgcgc tgggacgacc tggcccgcca cccccgcgcc accgtcaccg
      661 gccacatcgg gtcgggcacc cgcctggccg ccacccttcc ggccccctga ccgccgccag
      721 acgacggtcg agcgggccgg gtaagggcgc cggggttccg gttggggtag ggcgtgggtc
      781 gggcaaggca gggaggggtt gtgggcgagc cgaagcaccc gcagctccgg gtcggctacg
      841 ctcccccacc gggggacccg gcacaggacc gggtgggtga agcgtggctc ccggtgggga
      901 aggcgcgggc cgcgccggcg aggggttcga gaccggggcg atgacgtacg gggcgagctc
      961 ggaacgcggg tccggacccg gacccatcac gccggacggg tgtgcggtgg agctgtacgc
     1021 gctgctgccg ccgatgggcg aacccgaggt cgtgcatgcc gcgatccccg ccggagcctc
     1081 gatcctggag ctgggggccg gggccggccg gatgacccac cggctggtgg agctcggcca
     1141 tccggtggtg gcggtggacg agtcgccgga gatgctggag cgcatccatg gagccgcgcc
     1201 gggaaccccg gcggaagcgc gaccgaccgg ggcggccgag gccggggtgg agaccgtcca
     1261 ggcccggatc caggacctcg acctcaatcg ccgcttcgac gccgtgctgc tggcctcgtt
     1321 cctggtcaac acgcccgacg acgatctgcg gcaccggttc ctggcggcgt gccggaacca
     1381 cctgggagac ggcggctgcg tgctcgtgca gcgccaccgg ccggggtggt ttgacgaggc
     1441 gaccgaggag gagcgcacca gcggcggggt caccttccgg ctgcgcgacc ttcgccggcc
     1501 gggacccggg ctgctcgcgg ccaccgccga gtaccgggtc ggggagcggg tgtggaccca
     1561 cgcgttcacc acccggcggc tcgacgacgg cgagctggcg gccgccctgg ccgaggccgg
     1621 gctggccgtc gacgcctacc tgaccggcga cggctcctgg gtcagggcgg tcccggcgtc
     1681 gcgcggggac gcgctcgggc gctggcccag gtaggcccgg gcgatcatcg tcaccacgtg
     1741 gtagacggcg tcctcggccg gggcgagcgg acccggctgg aaatggggtg aggccatgcc
     1801 ccgctgcacc gactcgcatg ccgcccagtc ctggcggttg gtccggtccc agaagtcgac
     1861 cgcgtacgcc gggtcgaagc cgggccgctc ggccgcctcg ggcgggaagt accaggtgca
     1921 ggccacgtgg ctgaggtcgg ggccgagcgg ctccaccagg tgggtcatca ggtagtcggg
     1981 gtggagactg agcaggaggt tcgggaacag cccgaggtag gcgacggtcc gcagccgctc
     2041 gtcgtccagc cccggcatgg ggacgccgtc gctgtggccg tccagcgaca tggtgacggc
     2101 atggtccttc aggtccatgg tgccgccgac ccaggcgccg tcgagctcga agttgtcgcc
     2161 gctggcgggc gggctgacct ggcagagctc ggggtggatg agcgggcagt ggtagcactc
     2221 gtggtagttc tcgaggatga ccttccagtt gcaggccagg tcgtactcat gggtggccag
     2281 ggggaccagc cgctccgggc ggtagggggc gaccagctgg tcaagggccc cgacatgctc
     2341 ggcgaacggc ggggcgtcgc ccgacccgtt gacgaacagg aacccgtgcc agctctccag
     2401 cggcagctcg accagcccgt gctcggccgg gcggaagtcg ccgtggtcgc ggaacccggg
     2461 ggcggcccgc agggccccgt ccaggtcgta ggtccaggcg tggtaggggc agaggacggt
     2521 ccgcctgttg gtccgttcgc caacgccgag cagctcgtgg ccccggtggc ggcaggtgtt
     2581 ggcgaaggcc cgcatccgcc cgtcggtccc gcgggccagc agcaccccgg ccccgccgac
     2641 cttgaccgcc cgctgggccc ccgtgcccgc gaggtcgccc tcgcggccca cgcaggtcca
     2701 ggacccggcg aagaagcgct cctgctcgaa ggcgaacacc cgcggcgaga cataggcgtc
     2761 ccgcgggagc atgcggctct ggccgaacgg ccgcagggtc ggctcgaggt cggccgggtc
     2821 gaagggcgga gcggtggcgt ccagcatggt cggacctcca acctacttca actgactctt
     2881 cagtcagtat aagccggggc ggacctcggg aaccagccgg aggcggatac actgcggcgg
     2941 tggcgcggat caaggcggac gtccgccggg aggagatcct caaggcgacc tgccgtgagg
     3001 tgatcgcccg cgggttcgcg agcacccggg tgggggacgt ggcggccgcc ctcggggtgt
     3061 cgaccgggct ggtgttctac cacttctcca agaaggagac cctgctcggg gaggcgttcc
     3121 gccacgccgc cggcgaggac ctggaccggc tgtcggcaac ggccgccggt gacggcagcg
     3181 ccgcccagcg gctggaccgg atcctgcggc tgtactcccc cgccggctcc tcggaggcgt
     3241 ggctgctctg gatcgacgcc tggggccagg ccctgcgcag ccccgagctg gccgaggtgt
     3301 cgcgccagct cgacctgcgc tggaagcaga ccctggccgc ggtgatccgc gagggggtgg
     3361 cggccggcga gttcacctgc cccgaccccg acgccgccgc ctggcggctc accgccctgc
     3421 tcgacgggct cggggtccag gtcacggtcc acggcagcgt ccccgacgcc cgcctggtcg
     3481 actgggtccg ggcggccgcc gccgccgagc tggccgtcga cccgtcggtc ctggccggcg
     3541 acggccgccc atagacggcg cctgtcgcac cggcgcggga ggatcggacg ggaggcaacc
     3601 cgacccgagg aggtccgccc atggccgtcg gagtccaggt caccttcgac gcggccgacc
     3661 cgcaggccct ggccgccttc tggggggaga cgctcggcta cgtcgagcaa gaccctcccg
     3721 cggggttcga cagctgggag gcgtgggccg tggccaacga cgtgccccgc gagaagtggg
     3781 gcgactacga cgcccgggtc gaccccgacg gcgccggccc gcggctgtac ttccagcggg
     3841 tgcccgagcc caagacggcc aagaaccggg tccacctgga cctggaggtc agcgggcggg
     3901 gc
//
LOCUS       DASSWR010000600         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_869907,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000600 DASSWR010000000
VERSION     DASSWR010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869907"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFO47_09980"
     CDS             complement(<1..499)
                     /locus_tag="VFO47_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET8789063.1"
                     /translation="MTAPADVEPRFNLRRFLLSGDGWPYLLVPFIPLAIILEVVHAGA
                     TPIFLAAALGVIPTAALMGRSTEELAARSGPGIGGFLNVTFGNFPELVIALFALNEGR
                     QEVVKASVVGSILGNILLVMGAAMLAGGRRRDRQYFDRTAAGVQSLMLLLAAVALVMP
                     AIFELV"
     gene            complement(512..1780)
                     /locus_tag="VFO47_09985"
     CDS             complement(512..1780)
                     /locus_tag="VFO47_09985"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET8789064.1"
                     /translation="MLTFGAAISTAPDHQQALDEAIPAALDQLGGAPDLVICFFSMEH
                     AGAAAGIALSLSERTGTSAILGCTAQGVIGDGRELEGEPGLAVWMARLPGVDVRPFAL
                     RVLPLEDGVGIGGWPDLPEDERASVLLLADPFTFPADSFLERLNAEQPGLPVVGGMVS
                     GAMEPGRHRLLCGTEVLDGGAAGVALVGPVDIRAVVSQGCRPVGSPFAVTRGEGNIVH
                     ELGGQPAVDRLRQLLASLDQHEQELLRGGGLQVGQVIDEHKASFDRGDFLVRGLLGAD
                     PETGSIAVGDSVEVGQTLQFHVRDAAAADEDLELLLTPVSRWRPRGVLLFSCNGRGRH
                     FFGEPDHDAARVAAATDAAPMAGFFAQGEMGPIGGRNFLHTFTASMAVFCEPRDPVPA
                     LDRAAEAAPASEQPPVPSQPPQAPEAPEPV"
     gene            complement(1809..>2905)
                     /locus_tag="VFO47_09990"
     CDS             complement(1809..>2905)
                     /locus_tag="VFO47_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET8789065.1"
                     /translation="AVFVAGWVVFVAAWSGPAGAGPDGRGARVAGARAADLDAFRNEL
                     LVWAPGGFSEREVGRVRDSTRVAAISAVRTGTLPAASGRRGYRVVPVEAMAVDTNAYT
                     AAAGRPGRKLATLLPKGAVLSKTGAKLRKVKSGGKLKLSGNRTVAVSGVVDDALLGGY
                     EVALERTLARRYGIQRAAYLLVRPRGSVDGLQTELGKLLKGRAVRFVTPGDRPYVRGG
                     DGLLPLAQVKARFGEFALPSLHRGRPDPKWTRANLVTRKLPVLGRVRCHRLVVDDLGK
                     AMADLQRQGLRGQVDVGAFRRQGGCWSPRLLRDARGGKLSRHAWGIAVALKVTGDERL
                     IRTMAQHGFAWGGGFARPDATHFEWVGRGS"
BASE COUNT          396 a         1064 c         1113 g          332 t
ORIGIN      
        1 ccacgagctc gaagatcgcc ggcatgacca gggccacggc ggccagcagc agcatcagcg
       61 actggacgcc ggcggcggtg cggtcgaagt actggcggtc gcggcgccgc cccccggcca
      121 gcatcgccgc ccccatgacg agcaggatgt tgccgaggat ggagccgacg accgacgcct
      181 tgacgacctc ctggcggccc tcgttcagcg cgaacagggc gatgacgagc tcggggaagt
      241 tgccgaaggt gacgttgagg aagccgccga tgccgggtcc ggagcgggcg gccagctcct
      301 cggtggaccg ccccatgagg gcggcggtcg ggatcacccc gagggcggcc gcgaggaaga
      361 tcggcgtggc cccggcgtgg acgacctcga ggatgatggc cagggggatg aacggcacca
      421 gcaggtacgg ccagccgtcc ccgctgagca ggaagcgacg gaggttgaag cggggctcga
      481 cgtcggcggg ggcggtcatc ggcggctgct cctagacggg ctccggggcc tccggggcct
      541 gcggcggctg ggacgggacc ggtggctgtt cgctggccgg ggcggcttcc gcggcacggt
      601 cgagggccgg caccgggtcg cggggctcgc agaacaccgc catgctggcg gtgaaggtgt
      661 gcaggaagtt gcggccgccg atcggtccca tctcgccctg ggcgaagaag ccggccatcg
      721 gcgcggcgtc ggtggcggcg gccacccggg cggcgtcgtg gtcgggttcg ccgaagaagt
      781 gccggccccg gccgttgcag gagaacagca gcacgccccg cggccgccag cgggacacgg
      841 gggtcagcag cagctcgagg tcctcgtcgg cggcggcggc gtcgcggacg tggaactgca
      901 gggtctggcc cacctcgacg ctgtccccga cggcgatcga gccggtctcc gggtcggccc
      961 cgagcaggcc cctgaccagg aagtcgcccc ggtcgaagct ggccttgtgc tcgtcgatga
     1021 cctggccgac ctggaggccg ccgccccgca gcagctcctg ctcgtgctgg tcgaggctgg
     1081 ccagcagctg gcgcagccgg tcgaccgctg gctggccgcc cagctcgtgg acgatgttgc
     1141 cctcgccccg ggtgacggcg aacggcgagc ccaccgggcg gcagccctgg ctgaccacgg
     1201 cccggatgtc gacgggcccg accagggcga ccccggcggc gccgccgtcg agcacctcgg
     1261 tgccgcagag cagccggtgc ctccccggct ccatcgctcc cgagaccatc ccgccgacca
     1321 ccggcaggcc gggctgctcg gcgttgaggc gctccaggaa gctgtcggcc gggaaggtga
     1381 aggggtcggc cagcagcagc acgctggccc gttcgtcctc gggcaggtcg ggccagccgc
     1441 cgatgccgac gccgtcctca aggggcagca cccgcagggc gaacggccgg acgtcgaccc
     1501 cgggcagcct ggccatccag acggccaggc ccggctcccc ctccagctcg cggccgtcgc
     1561 cgatgacccc ctgggcggtg cagccgagga tggccgaggt gccggtgcgc tccgacaggc
     1621 tcagggcgat gccggcggcg gcgccggcgt gctccatcga gaagaagcag atcaccaggt
     1681 cgggagcgcc gccgagctgg tcgagggcgg ccgggatggc ctcatcgagg gcctgctggt
     1741 ggtcgggtgc ggtggagatg gcggcgccga aggtgagcat ggcgcaaggc tacccggttt
     1801 cgaccccgct aggatccccg gccgacccac tcgaaatggg tcgcgtcggg acgggcgaaa
     1861 ccgccgcccc aggcgaagcc gtgctgggcc atggtccgga tcaggcgctc gtccccggtg
     1921 accttgaggg ccacggcgat gccccaggcg tggcgggaca gcttgccccc gcgggcgtcg
     1981 cgcagcagcc gggggctcca gcagccgccc tggcgccgga aggcgcccac gtcgacctgc
     2041 ccgcggaggc cctggcgctg caggtcggcc atggccttgc ccaggtcgtc gacgaccagg
     2101 cggtggcagc gcacccgccc gagcacgggc agcttcctgg tcaccaggtt ggcccgcgtc
     2161 cacttcgggt cgggccggcc acggtgcagg ctcggcaggg cgaactcgcc gaagcgggcc
     2221 ttgacctggg ccaggggcag gaggccgtcg cccccgcgga cgtacgggcg gtcgccgggg
     2281 gtgacgaacc ggaccgcccg gcccttgagc agcttgccga gctcggtctg gaggccgtcg
     2341 accgacccgc gcggcctgac cagcaggtag gcggcgcgct ggatgccgta gcgccgggcc
     2401 agggtgcgct caagggccac ctcgtagccg cccagcaggg cgtcgtcgac caccccgctg
     2461 acggccacgg tgcggttgcc ggacagcttc agcttgccgc ccgacttgac cttgcggagc
     2521 ttggccccgg tcttggagag gaccgccccc ttgggcagca gggtggcgag cttgcgcccc
     2581 ggccggccgg cggcggcggt ataggcgttg gtgtcgaccg ccatcgcctc caccgggacc
     2641 acccggtagc cgcgccgccc gctggctgcc ggcagcgtgc cggtccgaac ggcgctgatg
     2701 gcggccaccc gggtcgagtc gcgcacccgc ccgacctcac gctcggagaa cccgcccggc
     2761 gcccagacca gcaactcgtt ccggaacgcg tccagatcgg cggcccgggc cccggccacc
     2821 cgcgcccccc gcccgtcagg cccggccccg gccggcccgc tccaggcagc aacgaacacg
     2881 acccacccgg ccacgaacac ggcca
//
LOCUS       DASSWR010000601         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_841444,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000601 DASSWR010000000
VERSION     DASSWR010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841444"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFO47_09995"
     CDS             <1..791
                     /locus_tag="VFO47_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8789066.1"
                     /translation="EAHRALGQLEAAEAAYQLASRWGMEPQPGLALLRLAQGRTEAAE
                     AAIRRVLGETGDRLHRSRLLPAATEILLAAGNTGAAADAAAELAGIAGDYGTPALLAA
                     AGHARGAVLLATGDAGAAVATLRGAWRAWRDLGVPYEAARVRVLVGLACRALGDTDAA
                     AMELDAARTVLAGLGAVPDLDRLDRLAGAAGPGASPGAGGLTGRELEVLRLVAAGKTN
                     HAIASALHLADKTVHRHVSNIYSKLGVSSRAAATAYAYEHDLVR"
     gene            911..2008
                     /locus_tag="VFO47_10000"
     CDS             911..2008
                     /locus_tag="VFO47_10000"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8789067.1"
                     /translation="MDPERFETVIVGGGQAGLATGWHLARRGRPFVILDAGERVGDQW
                     RRRWDSLRLFTPARYSGLPGMGFPAPAWHYPTKDEMAGYLEAYAARFQLPVRGGVRVD
                     GLTRLGDRFLVTAGERRFEAANVVVASGAYHTPRVPSFAVELDPAIVQLHSSGYRHPA
                     QLREGGVLVVGAANSGAEIALEASRAHPVWLSGRHPGSEPTRAGSRLDRLFTPPFWFA
                     INHVLSVGTPIGRKLRPKLMGVGTPLARVKPRDLKAAGVERVPRTAGVRDGLPLLEDG
                     RVLEVANVVWGTGFGHDFSWIDLPVFDAGGEPRHDRGVVEDQPGLYFVGLFFLSSVAS
                     SLVGGVGRDAAHIAERIAARVPSEEGLPAAR"
     gene            2041..>2829
                     /locus_tag="VFO47_10005"
     CDS             2041..>2829
                     /locus_tag="VFO47_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8789068.1"
                     /translation="MHESESLLLALMAAVAGLSVLARVVGVPYPILLVLGGLVLGFVP
                     GVPLVELPPELVLVVFLPPLLYWAGFFSSPRDLRAEARPISLAAVGLVLATMVAVAAA
                     AHALVDGMSWPAAFALGAIVSPTDPLAATAIGRRLGVPRRLITVLEGESLVNDATALV
                     AYRVAVAAAVSGTFVAWQAGLRFVVGVAGGVVIGLLVGWLVAELRRRLEDPVVEIVLS
                     VVTGYVAYLPADQLGVSGVLAAVAGLSVLARVVGVPYPILLVLGG"
BASE COUNT          278 a         1092 c         1071 g          388 t
ORIGIN      
        1 ccgaggccca ccgggccctc ggccagctcg aggcggccga ggccgcctac cagctggcca
       61 gccggtgggg catggagccg cagcccgggc tggccctgct gcgcctggcc cagggccgga
      121 ccgaggccgc cgaggcggcg atccgccgcg tgctcggcga gaccggcgac cggctgcacc
      181 gctcccggct gcttccggcg gccaccgaga tcctgctcgc ggccggcaac accggggcgg
      241 ccgccgacgc cgccgccgag ctggccggga tcgccggcga ctacgggacg ccggccctgc
      301 tggcggccgc cgggcacgcc cggggggcgg tgctgctggc caccggcgac gctggggccg
      361 ccgtggccac cctgcggggt gcctggcggg cctggcgcga cctgggggtc ccgtacgagg
      421 cggcgcgggt ccgggtgctg gtcggcctgg cctgccgggc cctcggcgac accgacgccg
      481 ccgccatgga gctcgacgcc gcccggaccg tgctggccgg gctgggcgcc gtccccgacc
      541 tggaccggct cgaccggctg gctggggccg ccggcccggg cgcatccccc ggggccggcg
      601 gactgaccgg acgcgagctg gaggtgctgc ggctggtggc cgcgggcaag accaaccacg
      661 ccatcgcctc cgccctgcac ctggccgaca agacggtcca ccgccacgtc agcaacatct
      721 acagcaagct gggcgtctcc tcccgggccg ccgccaccgc ctacgcctac gagcacgacc
      781 tggtccgctg acggggagaa gtacccatgg cagcgcggtc cgggtcgagg gcttctcccg
      841 atgccgcccc tgggtcccca ccctaggctc gggccaaccc cacgaggagg cggcgatgcg
      901 cagccacggc atggacccgg agcggttcga gacggtgatc gtcggcggcg gccaggccgg
      961 gctggccacc ggctggcacc tggccaggcg gggccggccg ttcgtgatcc tggacgcggg
     1021 cgagcgggtc ggcgaccagt ggcgccggcg ctgggactcg ctgcggctgt tcaccccggc
     1081 ccgctacagc ggcctgcccg gcatgggctt ccccgccccg gcctggcact acccgaccaa
     1141 ggacgagatg gccggctacc tggaggccta cgcggcccgg ttccagctcc cggtccgcgg
     1201 cggcgtccgg gtcgacgggc tcacccgcct cggcgaccgg ttcctggtca ccgccggcga
     1261 gcgccgcttc gaggcggcca acgtggtcgt ggcctcgggg gcctaccaca ccccccgggt
     1321 cccgtccttc gccgtcgagc tcgacccggc catcgtgcag ctccactcca gcggctaccg
     1381 gcacccggcc cagctccgcg agggcggcgt cctggtcgtc ggcgcggcca actcgggggc
     1441 cgagatcgcc ctggaggcct cccgggccca tccggtgtgg ctgtccggcc ggcacccggg
     1501 cagcgagccg acccgagccg gctcccggct ggaccggctg ttcaccccgc cgttctggtt
     1561 cgccatcaac cacgtgctga gtgtggggac gccgatcggc cgcaagctcc gccccaagct
     1621 gatgggggtg ggcacgcccc tggcccgggt caagccccgc gacctcaagg ccgccggggt
     1681 cgagcgggtg ccccgcacgg ccggggtccg cgacggcctc ccgctgctgg aggacgggcg
     1741 ggtcctggag gtggccaacg tcgtgtgggg caccggcttc gggcacgact tctcctggat
     1801 cgaccttccg gtgttcgacg ccggcggcga accccggcac gaccgcggcg tggtcgagga
     1861 ccagcccggg ctgtacttcg tgggcctgtt cttcctctcg tcggtcgcct cgtccctggt
     1921 cggcggggtc ggccgcgacg ccgcccacat cgccgaacgg atcgccgccc gcgtcccttc
     1981 ggaggaaggc cttccggcgg cccgctaagg tggcaacgag cacccgtcca ggagggctcg
     2041 ttgcacgaga gcgaatcgct gctgctggcg ttgatggccg ccgtggccgg gctgagcgtc
     2101 ctggcccggg tggtcggcgt gccctacccg atcctgctcg tcctcggcgg cctggtgctc
     2161 gggttcgtgc ccggcgtgcc gctcgtcgag ctgccgcccg agctggtcct ggtggtgttc
     2221 ctgccgccgc tgctgtactg ggccggcttc ttctcctcgc cccgcgacct gcgggccgag
     2281 gcccggccga tctcgctggc cgcggtcggg ctggtcctgg cgaccatggt cgcggtggcc
     2341 gcggccgccc acgcgctggt cgacgggatg agctggccgg cggcgttcgc gctgggggcg
     2401 atcgtctcgc cgaccgaccc gctggccgcc accgccatcg gccgccgcct gggcgtgccc
     2461 cgccggctca tcaccgtgct cgagggtgag agcctggtca acgacgccac cgccctggtc
     2521 gcctaccggg tcgcggtggc cgccgcggtc agcgggacct tcgtcgcctg gcaggccggg
     2581 ctgcggttcg tggtcggcgt ggccggcggc gtggtcatcg ggctgctggt cggctggctg
     2641 gtcgccgagc tgcggcgccg gctcgaggac ccggtggtcg agatcgtgct ctcggtggtc
     2701 accgggtacg tcgcctacct gccggccgac cagctcggcg tttccggggt cctggccgcc
     2761 gtggccgggc tgagcgtcct ggcccgggtg gtcggcgtgc cctacccgat cctgctcgtc
     2821 ctcggcggc
//
LOCUS       DASSWR010000602         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1094034, whole genome shotgun sequence.
ACCESSION   DASSWR010000602 DASSWR010000000
VERSION     DASSWR010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094034"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..732)
                     /locus_tag="VFO47_10010"
     CDS             complement(91..732)
                     /locus_tag="VFO47_10010"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET8789069.1"
                     /translation="MNPASVVEWLEGLQGPWAYLGVFVVAFAEHALFVGTFLPGEVIL
                     PLTGVAAAATGVVAWPVAAACATLGGFLADQLTYWLGRWLGRRRGERAGPPGRFARPA
                     AVVRELYRRHGALALVLGRFVAGVAPFVAPGAGWAGMPYRRFLPANALANALWGPVLV
                     GVGYLFGVQAARLDGPWRWAGLAVTGTVLALAFLRLRSIGADLAKATDRSSGQ"
     gene            complement(729..2528)
                     /locus_tag="VFO47_10015"
     CDS             complement(729..2528)
                     /locus_tag="VFO47_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HET8789070.1"
                     /translation="MSGACAVAADERLAAYDFGPDRPFQAGRLDAGLSLLRAAKVLDT
                     GDLLAFDPAGDADLELIHDRDYLDALARFSAPGEAAADPAEAARWGLVGDNRPFPGMD
                     AAARLVAGATTAALDAVAGGELAHVFAPVAGLHHAQRRRAVGFCLVNDVAVAIARCTR
                     AWPLRVLYVDLDAHHGDGVQAAFYDDPRVCTVSLHETGRYLFPGSGEVHELGRPPGVG
                     RSVNLPLEPRTGDDGFLAAFDAVVEPLAAAFRPDVLVTQNGCDGHADDPLSDLDLSLR
                     GLRALARRLHRLAHRHCQGRWVATGGGGYDLDRVVPRAWALLWSELSGRRLPARMTAD
                     WLESRRRQRRRAPAPGSREREERNQRTVRALRRLVLPPAVRVVYPLAGRGGGAGRLPR
                     WEPRAGEHLAPGGALVLRDRSPASLLRRLEVDPGIHAFVRDPAQEREVLARIAEEPNG
                     EVVVAHTPEGVVVGYVAVLDPEPGSRFASLPGLLEVGAVEVATGWRRGGVAGALLRFA
                     VEPDPFEDLILIASGLAWHWDLEQSGLSATAYRRVLRRVFEVAGFEALQTDEPEIAYH
                     PANLFVARVGSRVPPARRVAFTSRLVRGHHAMP"
     gene            complement(2525..>2867)
                     /locus_tag="VFO47_10020"
     CDS             complement(2525..>2867)
                     /locus_tag="VFO47_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8789071.1"
                     /translation="IGCLPVVGDDRLVGILTESDLFTVLLRLLGGGEPSSRITLVLPD
                     VPGALGRAMTVVGELGVNLLTVVTEPGPEPGTRGVVLRAGTINAAPLVAALVAAGFPA
                     TGPGRPELDDR"
BASE COUNT          387 a         1108 c         1106 g          266 t
ORIGIN      
        1 gcgccatgtc ctccccggtc cgcatcacag agcgggtatg aaacaggttc ggagggtgat
       61 gcctcaacta ccggtcaagc atcacgcacg tcactggccc gacgaacggt ccgtcgcctt
      121 ggcgaggtcg gcgccgatcg agcgcaggcg caggaaggcc agggccagga cggtcccggt
      181 gacggccaga ccggcccagc gccagggtcc gtcgagccgg gccgcctgca cgccgaagag
      241 gtagccgacg cccaccagga ccggccccca gagggcgttg gccagggcgt tggcgggcag
      301 gaagcggcgg tacggcatgc cggcccagcc ggccccgggg gccacgaacg gcgccacccc
      361 ggcgacgaac cgccccagga ccagggccag ggcgccgtgg cggcggtaca gctcgcgcac
      421 gaccgcggcc ggcctggcga agcggcccgg ggggccggcc cgctccccgc gccggcgtcc
      481 cagccaccgg cccagccagt aggtgagctg gtcggccagg aagccgccga gggtggcgca
      541 ggccgcggcc accggccagg ccaccacccc ggtggcggcg gcggccaccc cggtgagggg
      601 caggatcacc tccccgggca ggaaggtccc gacgaacaac gcgtgctcgg cgaacgcgac
      661 cacgaacacg cccaggtacg cccatgggcc ctggagcccc tccagccact cgacgaccga
      721 ggccgggttc acggcatggc gtggtggccc cggaccagcc ggctggtgaa ggcgacccgg
      781 cgggccggcg gcacccggct gccgacgcgg gccacgaaca ggttggccgg gtggtaggcg
      841 atctccggct cgtcggtctg gagcgcctcg aaccccgcga cctcgaacac ccgccgcagg
      901 acccgccggt aggcggtcgc cgacaggccg ctctgctcca ggtcccagtg ccaggccagg
      961 ccggaggcga tcaggatgag gtcctcgaag gggtcgggct cgacggcgaa gcgcagcagg
     1021 gcgccggcca ccccgccccg ccgccagccg gtggccacct cgacggcgcc gacctcgagc
     1081 aggccgggga ggctggcgaa gcggctgccg ggctcggggt cgagcacggc cacgtacccg
     1141 accaccaccc cctcgggcgt gtgggcgacc accacctcgc cgttgggttc ctcggcgatc
     1201 cgggccagca cctcccgctc ctgggcgggg tcgcggacga aggcgtggat gcccgggtcg
     1261 acctcgagcc ggcgcagcag cgaggccggg ctgcggtcgc ggagcaccag agcgccgccc
     1321 ggggccaggt gctcgccggc ccgcggctcc cacctcggca gccgcccggc cccgccgccc
     1381 cgcccggcca gcgggtagac gacccgcacg gccgggggca ggaccagccg ccgcagcgcc
     1441 cggacggtcc gctggttgcg ctcctcccgc tcccgcgacc cgggcgcggg ggcccgacgc
     1501 cgctggcgcc gccgcgactc cagccagtcg gccgtcatcc tcgccggcag gcggcggccg
     1561 ctcagctccg accagagcag cgcccaggcc ctcgggacca cccggtccag gtcgtagccg
     1621 ccacccccgg tggccaccca gcggccctgg cagtggcggt gggcgagccg gtgcagccgc
     1681 ctggccagcg cccggaggcc gcgcaggctc aggtcgaggt cgctgagcgg gtcgtcggcg
     1741 tggccgtcgc agccgttctg ggtgaccagc acgtcgggcc ggaacgcggc ggccagcggc
     1801 tcgaccacgg cgtcgaaggc ggccaggaag ccgtcgtcgc cggtccgcgg ctccaggggc
     1861 aggttgacgc tgcgccccac cccgggcggg cggcccagct cgtgcacctc gccggacccg
     1921 gggaacaggt agcggccggt ctcgtgcagg ctgaccgtgc acacccgcgg gtcgtcgtag
     1981 aaggcggcct ggacgccgtc gccgtggtgg gcgtccaggt cgacgtagag cacccgcagc
     2041 ggccaggccc gggtgcagcg ggcgatggcc acggcgacgt cgttgaccag gcagaacccg
     2101 acggcgcggc gccgctgggc gtggtggagg ccggccacgg gcgcgaacac gtgcgccagc
     2161 tcacccccgg cgaccgcgtc aagggcggcc gtggtcgccc ccgcgaccag ccgggccgcc
     2221 gcgtccatgc ccgggaacgg ccggttgtca ccgaccagcc cccagcgggc cgcctcggcc
     2281 gggtcggccg cggcctcccc cggcgccgag aagcgggcca gggcgtccag gtagtcgcgg
     2341 tcgtggatca gctccaggtc ggcgtcgccg gccgggtcga aggccagtag gtcgccggtg
     2401 tccagcacct tggccgcccg cagcagcgac aggccggcgt ccagccggcc ggcctggaag
     2461 gggcggtcgg gcccgaagtc gtaggcggcc agccgctcgt cggcggcgac ggcacaggcg
     2521 ccgctcaccg gtcgtccagc tcgggccggc cagggccggt ggcagggaag ccggcggcca
     2581 ccagggcggc caccagcggg gcggcgttga tggtcccggc ccgcaggacg accccgcggg
     2641 tgccgggctc cgggccgggc tcggtgacca ccgtgagcag gttgaccccg agctcgccga
     2701 ccaccgtcat ggcccgcccc agggcaccgg gcacgtccgg caggaccagg gtgatgcggc
     2761 tcgacggctc gcccccgccg agcagccgca gcagcaccgt gaacaggtcg gactcggtga
     2821 ggatgccgac cagccggtcg tcgccgacca ccggcaggca gccgatg
//
LOCUS       DASSWR010000603         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_615932,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000603 DASSWR010000000
VERSION     DASSWR010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615932"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFO47_10025"
     CDS             complement(<1..328)
                     /locus_tag="VFO47_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8789072.1"
                     /translation="MNVRSIFRPWVVRVDGDDCLADAAERMQNEQVGSVVVTVGGRFV
                     GILTERDLTRAIGDGADPRDATAADFMTAAPAAVHLDADIHEVASTMFEFECRHLPVM
                     SEDDSQV"
     gene            complement(371..>2535)
                     /locus_tag="VFO47_10030"
     CDS             complement(371..>2535)
                     /locus_tag="VFO47_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET8789073.1"
                     /translation="TGAATRLGGIARLTGEVVRRPTPLRIQLNRVVRVISVFAVVTGA
                     TFFAVMVGMGDSPGDGFLFSVGVIVALVPEGLLPTLTLSLAMSATRMARRGALVRHLE
                     AVETLGSTTVICSDKTGTLTANQMTARAVAVAGAHYRASGIGYDPSGALLAGERPLTP
                     RERAEVQDLLRAAVLCNDAHLERRDGRWRCVGDPTEGALLALAAKGGLTRQPTERAAP
                     RVREYPFESARRRMSTAHRLATGRLELFTKGSPEAVLEACTSVRVGAHRVELDPGREA
                     AVLADVEALAAEGLRVLALARRELDGQAPSIAAAAEQDLELLGLVGMADPVRPEVPEA
                     IARCRRAGIRVVMVTGDHPATAAAVAAKAGLANGRVVLGADLPAGDAALADLLEGGVS
                     VLARIAPEQKLRIAQALQSRGEVVAMTGDGVNDAPALRRADIGVAMGVVGTDVARSAA
                     DIVLLDDNFAHIVEAVEEGRAAFDNIKRFLTYHLTDNVAELAPFALWALTAGRVPLAL
                     SVLQILALDIGTDLLPALALGAEPPEPGVMERQPRTRSARSLDRAVLARAFGFLGPVE
                     AVASLALLPVGAALFFGWHPGTPLPAGGTALASLSTMVFAAIVLAQMANAFECRSSRM
                     SLRTLGLATNRLLVGAVAVEGLALLGFVYVGPVRALLGHQPLTLAQWLPVLVAPVLLF
                     AAEEARKAVVRRHRRVVAGPTGPQPTGRKALRRRTGPG"
BASE COUNT          361 a          953 c          919 g          302 t
ORIGIN      
        1 tcacctggct gtcgtcctcg gacatgaccg gcaggtggcg gcattcgaac tcgaacatgg
       61 tcgaggccac ctcgtggatg tcggcgtcga ggtggacggc ggcgggcgcg gccgtcatga
      121 agtcggcggc ggtggcgtcc cgggggtcgg ccccgtcgcc gatcgccctg gtgaggtccc
      181 gctcggtcag gatgcccacg aaccggccgc cgacggtgac gaccacggaa ccaacctgct
      241 cgttctgcat ccgctcggcg gcgtcggcaa ggcagtcatc gccatcgacg cgaaccaccc
      301 agggacggaa gatcgacctg acgttcatct cgcctcacct catctggacc tgcgtcctca
      361 tctgccaacg ctagccgggg ccggtccgcc gcctcagggc cttccggccc gttggctgcg
      421 ggccggtcgg tccagccacc acccgacggt gccggcgaac gaccgccttg cgggcctcct
      481 cggccgcgaa cagcaggacc ggggcgacca gcacggggag ccactgggcc agggtcagcg
      541 gctggtggcc gagcagcgct cgtaccggcc ccacgtagac gaacccgagc agggccaggc
      601 cctccacggc gaccgccccc accagcaggc ggttggtcgc caggccgagg gtgcgcagtg
      661 acatccggga gctgcggcac tcgaaggcgt tggccatctg ggccagcacg atggcggcga
      721 acaccatggt cgataaggag gccagcgccg ttccgccggc cggcaggggg gtccccggat
      781 gccagccgaa gaacagcgcc gccccgaccg gcagcagggc cagcgaggcg accgcctcca
      841 ccggaccgag gaacccgaag gcgcgggcga gcacggcgcg gtcgagcgac cgggccgacc
      901 gtgtccgcgg ttgccgctcc atcaccccgg gttcgggtgg ttcggcgccg agggccaggg
      961 ccggcagcag gtcggtgccg atgtccaggg cgaggatctg cagcaccgac agggccaagg
     1021 ggacgcggcc ggcggtcagg gcccagagcg cgaacggggc cagctcggcg acgttgtcgg
     1081 tgaggtggta ggtgagaaag cgcttgatgt tgtcgaaggc tgcccgccct tcctccaccg
     1141 cctcgacgat gtgggcgaaa ttgtcgtcca gcaggacgat gtcggcggcc gagcgggcca
     1201 cgtcggtccc gaccacgccc atcgccacac cgatatcggc ccggcgcagc gccggggcgt
     1261 cgttgacacc gtcgccggtc atggccacga cctcgccgcg ggactggagc gcctgggcga
     1321 tgcggagctt ctgctcggga gcgatgcggg ccagcaccga cacgccgccc tcgaggaggt
     1381 cggcgagggc ggcgtcgccg gccgggaggt cggcgccgag caccacccgg ccgttggcca
     1441 gcccggcctt ggcggccacg gcggccgccg tggccgggtg gtcgccggtg accatgacca
     1501 cccggatccc ggcccgccgg cagcgggcga tcgcctcggg cacctcgggc cgcaccgggt
     1561 cggccatccc gaccagcccg agcagctcca ggtcctgctc ggcggccgcc gcgatcgacg
     1621 gcgcctggcc gtcgagctct cgccgggcca gggccaggac ccgcagcccc tcggcggcca
     1681 gggcctccac gtcggccagc acggccgcct cccggccggg atcgagctcg acccggtgcg
     1741 cgccgacccg gacggacgtg cacgcctcca ggaccgcctc cggcgacccc ttggtgaaca
     1801 gctccagtct ccctgtggcc agccggtgcg ccgtgctcat ccgccggcgg gccgactcga
     1861 aggggtactc ccgcacccgg ggagccgccc gctcggtcgg ctgcctggtc aggccgccct
     1921 tggcggccag ggcgagcagc gccccctcgg tcgggtcgcc gacacaacgc cagcggccgt
     1981 cgcgccgctc caggtgggcg tcgttgcaga gcacggccgc cctgagcagg tcctgtacct
     2041 cagcccgttc ccggggtgtc agcggccgtt ccccggccag cagcgccccc gacgggtcat
     2101 agccgatccc tgaggcgcgg tagtgcgcgc cggccacggc gaccgcccgg gcggtcatct
     2161 ggttggcggt cagggtgccg gtcttgtccg agcagatcac cgtggtcgaa cccagcgtct
     2221 cgaccgcctc caggtggcgg accagcgccc cccgccgggc catgcgggtc gcgctcatgg
     2281 ccagcgacag ggtcagggtg ggcagcagcc cctcggggac cagggcgacg atcacgccca
     2341 ccgagaacag gaacccgtca ccgggggagt cgcccatccc aaccatcacg gcgaagaacg
     2401 tcgcccccgt gaccacggcg aacaccgaga tgacgcgcac cactcggttg agctggatcc
     2461 gcagcggggt cggccgccga accacctcgc ccgtcaaccg ggcgatccct ccgagccggg
     2521 tcgccgctcc cgtga
//
LOCUS       DASSWR010000604         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1322275, whole genome shotgun sequence.
ACCESSION   DASSWR010000604 DASSWR010000000
VERSION     DASSWR010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322275"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VFO47_10035"
     CDS             <1..1075
                     /locus_tag="VFO47_10035"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789074.1"
                     /translation="PAGAIVSPTPSPGAGACETNGRVNAVAYLGGTIYLGGSFTSVDG
                     STRNRLAACDAATGTLLSWNPDANGVVRALKVSPAGTRVYVGGDFTAVGGATRARVAA
                     LNPTSGAAFSWDPYVNDSVKAITTSTTGSTVYVGGDFSSAEGAGRRRLAAFNATSGNI
                     STTFKPNVSNGTGNFATVLALAVHPNNQTLYFSGDFALVNGTSRRNAAAVSTGVATLR
                     AWSPASTADVAGDLVVSASGNTVFVGGRATGGYVQAYGPTAGGAPVWNVRTNGDVEAL
                     AVSSSILYVGGHFTTVGGTGRGHLAALSTSGGRLQGWAPTADGVFGAFGAAITSSRVA
                     FGGEFTTVAGEAHQGIVQFSGTP"
     gene            1095..1823
                     /locus_tag="VFO47_10040"
     CDS             1095..1823
                     /locus_tag="VFO47_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8789075.1"
                     /translation="MGTRLVNLVVDAASPAASARWWADALGWRVTLEQPDEVVVEPPE
                     PDGLGIPLVFVPVPEPKTGPNRVHLDLASPSAADQAALVARLTALGATPADVGQGEVP
                     WVVLADKDGNELCVLEPRDRYRGTGRLAAVVVAVADPPALARFWTEAAAWPVTEVNDR
                     FAALRHPSGTGPFLELIRSPAPKLGKNRLHLDLAPYPDDDPATEVARLRSLGATPADI
                     GQGEVTWTVLADPEGNELCVLSPR"
     gene            complement(1879..2676)
                     /locus_tag="VFO47_10045"
     CDS             complement(1879..2676)
                     /locus_tag="VFO47_10045"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET8789076.1"
                     /translation="MTDHRLVFVGGLHRSGTTPLARCLAAHRQISGFANTGAEEDEGQ
                     HLQTVYPPARAYGGPGRFARHPAAHLTESSPLLTPDTAARLLDQWRPHWDLARPVLVE
                     KSPPNLLMTRFLQGAFPDARFVMVVRHPAIVSLSTRKWARLSSLGALLDHWFAAHRRF
                     EDDAPFIHRLLVVKYEHLVADPERTLAEIAAFLELDGDIPAGGIDTRRSATYERQWAD
                     LAAGGPWRRERFRALCRRHEPAANHFGYSLLDLERADPFPVGSPRTP"
     gene            complement(2673..>3343)
                     /locus_tag="VFO47_10050"
     CDS             complement(2673..>3343)
                     /locus_tag="VFO47_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789077.1"
                     /translation="DVTISSALPHRLWRRGRRYALPPVAVSREIAALGRVEDTTLGES
                     RWWARLRERLGGAVGAGGATLDGTLERLDGHASTRLAFGTWHGDWGPWNLRATPGRLL
                     VWDWERSADGVPLGFDLLHFGYQTALQGLGQPPAVAATTGRDRAAPHLAPLGQRPGVE
                     ELLGDLYLLERLCRAAEAEVSAVTGRPDSVGAALLGVLDGRLVPGGSGGPPGWSPGRS
                     GGTG"
BASE COUNT          409 a         1320 c         1218 g          396 t
ORIGIN      
        1 cccggcgggg gcgatcgtca gccccacccc gagtcccggg gccggcgcct gcgagaccaa
       61 cggccgggtc aacgccgtcg cctacctggg cggcaccatc tacctcggcg gcagcttcac
      121 ctcggtcgac gggtcgaccc gcaaccggct ggccgcctgc gacgccgcca ccgggaccct
      181 gctctcctgg aaccccgacg ccaacggcgt ggtcagggcc ctcaaggtct cccccgccgg
      241 gacccgggtg tacgtgggcg gggacttcac cgcggtcggc ggggcgaccc gggcccgggt
      301 cgccgccctc aacccgacca gcggggccgc cttctcctgg gacccgtacg tcaacgacag
      361 cgtcaaggcg atcaccacct cgaccaccgg ctccaccgtc tacgtcggcg gcgacttcag
      421 ctcggccgag ggggcgggcc ggcggcgcct ggccgccttc aacgccacca gcggcaacat
      481 ctccaccacc ttcaagccga acgtctccaa cggcaccggc aacttcgcca ccgtgctggc
      541 cctggccgtc caccccaaca accagaccct ctacttcagc ggcgacttcg ccctggtcaa
      601 cggcacctcg cgccgcaacg cggccgccgt gtcgaccggc gtggccaccc tgcgggcctg
      661 gagccccgcc tccaccgccg acgtcgccgg cgacctggtg gtctcggcct cgggcaacac
      721 cgtgttcgtg ggcggccggg caaccggcgg ctacgtgcag gcctacggcc ccacggccgg
      781 cggtgccccg gtgtggaacg tccggaccaa cggcgacgtc gaggccctgg ccgtgtcctc
      841 ctcgatcctg tacgtgggtg gccacttcac caccgtcggc gggaccggcc gcggccacct
      901 ggccgccctg agcacctccg gcggccgcct ccagggctgg gcgcccaccg ccgacggcgt
      961 cttcggcgcc ttcggcgcgg ccatcaccag ctcccgggtc gccttcggcg gcgagttcac
     1021 caccgtcgcc ggcgaagccc accagggcat cgtgcagttc tccgggaccc cgtagggcgg
     1081 tcccggccgc cgccatgggc acccggctgg tcaacctcgt ggtcgacgcc gcctctccgg
     1141 cggcgtcggc ccgctggtgg gccgacgccc tcggctggcg ggtgaccctg gagcagcccg
     1201 acgaggtcgt ggtcgagccg ccggagcccg acggtcttgg catcccgctg gtgttcgtcc
     1261 ccgtgcccga gcccaagacc ggccccaacc gggtccacct cgacctggcc agcccgtccg
     1321 ccgccgacca ggcggccctg gtcgcgcgcc tgaccgcgct cggggccacc ccggccgacg
     1381 tcggccaggg cgaggtcccc tgggtggtcc tggccgacaa ggacggcaac gagctctgcg
     1441 tcctggagcc ccgcgaccgc taccgcggca ccggccgcct cgccgccgtg gtcgtcgccg
     1501 tcgccgaccc gccggccctg gcccgcttct ggaccgaggc cgccgcctgg cccgtgaccg
     1561 aggtcaacga ccgcttcgcc gccctccgcc acccgtcggg caccggcccg ttcctggagc
     1621 tgatccgctc gcccgccccc aagctgggca agaaccgcct ccacctcgac ctcgccccct
     1681 atccggacga cgaccccgcc accgaggtcg cccgcctccg ctccctcggc gccaccccgg
     1741 ccgacatcgg ccagggcgag gtcacctgga ccgtcctcgc cgaccccgag ggcaacgagc
     1801 tctgcgtgct ctccccgcgt tgatgacggc caaggtggag accgtcaacc gcgcccggca
     1861 ccatggccgc gccggccctc agggggtgcg gggcgagccc accgggaacg ggtcggcccg
     1921 ctccaggtcg aggaggctgt agccgaagtg gttggccgcc ggctcgtggc ggcggcagag
     1981 ggcgcggaag cgttcgcggc gccaggggcc gccggcggcc aggtcggccc actggcgctc
     2041 gtaggtggcg ctgcggcggg tgtcgatgcc gccggcgggg atgtcgccgt cgagctcgag
     2101 gaaggcggcg atctcggcca gggtgcgctc ggggtcggcg accaggtgct cgtacttgac
     2161 cacgagcagg cggtggatga agggggcgtc gtcctcgaac cggcggtggg cggcgaacca
     2221 gtggtcgagc agggcgccca gcgagctgag ccgggcccac ttgcgggtgg agaggctgac
     2281 gatggccggg tgcctgacga ccatgacgaa gcgggcgtcg gggaaggcgc cctggaggaa
     2341 ccgggtcatg agcaggttgg gcggggactt ctcgaccagg accggcctgg ccaggtccca
     2401 gtggggccgc cactggtcga gcaggcgggc ggccgtgtcc ggggtgagca ggggcgagct
     2461 ctcggtgagg tgggcggccg ggtggcgcgc gaaccggccc gggccgccgt aggccctggc
     2521 cggggggtag acggtctgga ggtgctggcc ctcgtcctcc tccgccccgg tgttcgcgaa
     2581 gccgctgatc tgtcgatggg cggccaggca gcgggccagc ggggtggtcc cgctgcggtg
     2641 cagcccgccc acgaacacca gccgatggtc ggtcatccgg tcccccccga tcgaccgggg
     2701 gaccaccctg gcggtccccc ggaccccccc ggtacgaggc ggccgtcgag gacgccgagc
     2761 agggcggccc cgaccgagtc gggtcgcccg gtcacggccg acacctcggc ctcggcggcc
     2821 cggcagagcc gctccagcag gtacaggtcg cccagcagct cctccacccc cggccgctgg
     2881 cccagcgggg ccaggtgcgg ggccgcccgg tcgcggccgg tggtggccgc caccgccggc
     2941 ggctggccga ggccctggag ggcggtctgg tagccgaaat ggagcaggtc gaagccgagc
     3001 ggcacgccgt cggcgctgcg ctcccagtcc cagaccagca gccgccccgg agtggcgcgc
     3061 aggttccacg gcccccagtc gccatgccag gtgccgaagg cgagccgggt cgaggcgtgc
     3121 ccgtccagcc gttccagggt cccgtcgagg gtcgcccccc cggccccgac ggccccgccg
     3181 agccgctccc gcagccgggc ccaccagcgg ctctccccca gcgtcgtgtc ctcgacccgg
     3241 ccgagggcgg cgatctcgcg ggagacggcc accgggggca gggcgtagcg gcggccgcgg
     3301 cgccacagcc ggtgcggcag ggccgagctg atggtgacgt cca
//
LOCUS       DASSWR010000605         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1266250, whole genome shotgun sequence.
ACCESSION   DASSWR010000605 DASSWR010000000
VERSION     DASSWR010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266250"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFO47_10055"
     CDS             complement(<1..391)
                     /locus_tag="VFO47_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET8789078.1"
                     /translation="MSIPYQSSGYRAKKLLLGPPLKTSQLAHERISKRVALAVFSSDP
                     ISSTAYATEEMLLVLVVAGTAAIGLALPLAAGVAVLLLILVLSYRQVIAAYPQGGGAY
                     MVTRDNLGAPFAATCAAALLVDYVLTVA"
     gene            506..1204
                     /locus_tag="VFO47_10060"
     CDS             506..1204
                     /locus_tag="VFO47_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET8789079.1"
                     /translation="MHVVIMGCGRVGSELAARLESGDHTVAVIDKNPAAFRMLRYQFK
                     GKKIQGYGFDEDILREAGIERAEAFAAVSNGDNSNIVAARLAREKFEVPLVVARIYDP
                     RRAEIYQRLGIPTVATVKWTTDQVMRVLIPDQIASDWRDPNAEIALITLVLPAEWAGW
                     KIEELEAEGHRRVVGVTRTGHARIVRPEMTLQEGDQVHLAVDDDGLQELRPRVLAARR
                     ERSDEPAAAREAAR"
     gene            1201..1953
                     /locus_tag="VFO47_10065"
     CDS             1201..1953
                     /locus_tag="VFO47_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018253518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET8789080.1"
                     /translation="MRVAIAGAGNVGQFIAADLLAAGHQVTMIEKDLHVLEVARPDLK
                     EAEWFPFDACEVLSLVMAGIAHADVMVAATGDDEDNLVISLLAKQEFAVPRVVARVNN
                     PKNHGLFTEAWGVDVAVSTPHILRSLVEEAVTVGSMIKVMQFERGHVSLHAVTLADRS
                     AAVGRTLRDLALPVDAAAVAVVREGHVVVPKEDTVFAAGDEVLVVTSEGSEGQVRELL
                     VGETEGGSPDQDDEDQEQPADEERPAPAERLQ"
     gene            complement(1945..2217)
                     /locus_tag="VFO47_10070"
     CDS             complement(1945..2217)
                     /locus_tag="VFO47_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789081.1"
                     /translation="MNDEITPMGNARARVRGRFQGQIRRIRIQPQAAVPTLEIMIEDD
                     TGRVAALFMGRRGIAGMECGGRLAIEGTPVATERGLTLYNPAYDLL"
     gene            complement(2214..2648)
                     /locus_tag="VFO47_10075"
     CDS             complement(2214..2648)
                     /locus_tag="VFO47_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET8789082.1"
                     /translation="MGETRRRGTRPDFKPDWEPFSLPEDEVLRRGGALFDDGMYWEAH
                     EVWEEVWRMRKGRPDRDFLKGIIQGAAGMWHATQGNYKGAVSVMSRAVEYLAPYRPAK
                     DGIDVERLSAAFEEAVAACRAAMDGGPAPRLRMVKLDLGGAR"
BASE COUNT          420 a          990 c         1037 g          377 t
ORIGIN      
        1 cggcgacggt cagcacgtag tcgaccagca gggcggccgc gcaggtcgcg gcgaacggcg
       61 cgcccaggtt gtcgcgggtc accatgtagg cgccgccgcc ctgggggtag gcggcgatca
      121 cctgccggta ggacaggacc aggatcagca gcagcaccgc caccccggcg gccagcggca
      181 gggccagccc gatggcggcc gtccccgcga ccaccagcac cagcagcatc tcctcggtgg
      241 cataggcggt cgaggagatc gggtcggagg agaacacggc cagcgcgacc cgcttggaga
      301 tccgctcgtg ggccagctgc gaggtcttca gcggcgggcc gagcaggagc ttcttggccc
      361 ggtagccgct ggactggtag gggatggaca cgacctcgat gctgcaggcc cggcgcaaag
      421 aacccgttaa ggccggccgg ccccggcgca aagagcccgt caagacccga tacgatggct
      481 cggtcgctca acaggaaggt gcctggtgca cgttgtgatc atgggctgtg gccgcgtcgg
      541 gtcggagctg gccgcccggt tggagtctgg cgaccacacg gtggcggtga tcgacaagaa
      601 ccccgccgcc ttccggatgc tgcgctatca gttcaagggc aagaagatcc agggctacgg
      661 cttcgacgag gacatcctcc gcgaggccgg gatcgagcgg gcggaggcgt tcgccgccgt
      721 gtccaacggc gacaactcca acatcgtggc cgcccgcctg gcccgggaga agttcgaggt
      781 cccgctggtg gtggcccgca tctacgaccc ccgccgggcc gagatctacc agcggctggg
      841 gatcccgacg gtggccaccg tgaagtggac caccgaccag gtgatgcggg tgctgatccc
      901 cgaccagatc gcttcggact ggcgcgaccc caacgccgag atcgccctga tcaccctggt
      961 cctgcccgcg gagtgggccg gctggaagat cgaggagctg gaggccgaag gccaccggcg
     1021 ggtggtcggg gtgacccgca ccgggcacgc ccggatcgtc aggccggaga tgaccctcca
     1081 ggagggcgac caggtccatc tggccgtcga cgacgacggc ctccaggagc tgcgcccgcg
     1141 ggtcctggcc gcccgccgcg agcgctcgga cgagccggcc gcggcccggg aggccgcccg
     1201 gtgagggtgg ccatcgccgg ggcgggcaac gtcggccagt tcatcgccgc cgacctgctg
     1261 gccgccggcc accaggtcac gatgatcgag aaggacctgc acgtcctgga ggtggcccgg
     1321 ccggacctga aggaggccga gtggttcccg ttcgacgcct gcgaggtcct gtcgctggtc
     1381 atggccggga tcgcccatgc cgacgtgatg gtcgcggcca ccggcgacga cgaggacaac
     1441 ctggtcatct cgctgctggc caagcaggag ttcgccgtgc cccgggtggt ggcccgggtc
     1501 aacaacccca agaaccacgg gctgttcacc gaggcctggg gggtcgacgt ggccgtgtcc
     1561 accccccaca tcctgcgctc cctggtcgag gaggccgtga ccgtcggcag catgatcaag
     1621 gtcatgcagt tcgagcgggg gcacgtgtcg ctgcacgccg tgaccctggc cgaccggtcg
     1681 gcggccgtcg gccggaccct gcgtgacctg gccctgccgg tggacgcggc cgcggtcgcg
     1741 gtggtcaggg agggccacgt ggtcgtgccc aaggaggaca cggtgttcgc cgccggcgac
     1801 gaggtcctgg tggtcacctc cgagggcagc gagggccagg tccgcgagct gctggtcggg
     1861 gagaccgagg gcgggtcgcc ggaccaggac gacgaggacc aggagcagcc cgcggacgag
     1921 gagcggccgg ctcccgccga gcggctacag taggtcgtag gcggggttgt agagggtcag
     1981 gccgcgctcg gtggccaccg gcgtcccctc gatggccagc cggcccccgc actccatccc
     2041 ggcgatgccg cgcctgccca tgaacaatgc cgccacccgg ccggtgtcgt cctcgatcat
     2101 gatctccagg gtggggacgg ccgcctgagg ctggatgcgg atccgccgga tctggccctg
     2161 gaaccgcccc cgcaccctgg cccgggcgtt ccccatgggg gtgatctcgt cgttcaccgg
     2221 gcgcctccga ggtcgagctt gaccatgcgc agccgtggcg ccgggccgcc gtccatggcc
     2281 gcccggcagg cggccacggc ctcctcgaag gcggccgaca ggcgctcgac gtcgatgccg
     2341 tccttggccg ggcggtaggg cgccaggtac tcgacggccc gcgacatcac gctcaccgcg
     2401 cccttgtagt tgccctgggt ggcgtgccac atcccggccg cgccctggat gatgcccttg
     2461 aggaaatcgc ggtcgggacg gcccttgcgc atccgccaga cctcctccca gacctcgtgc
     2521 gcctcccagt acatgccgtc gtcgaacagg gcgccgcccc ggcgcaggac ctcgtcctcg
     2581 ggcaggctga acggctccca gtccggcttg aagtccggcc gggtcccccg gcgtcgggtc
     2641 tcgcccatac gcagatggta cgccccgggc catggctcag gcgctcctgg ggggccctcg
     2701 ccagcgcgtg cgccgctgca gcaatcgcga gccgcgtcgt tcgaagcatc gggcggtaag
     2761 gaacggccac cgggcgcaaa gaagtcgtca aggtccggga tggcccggcg caacggccta
     2821 tcct
//
LOCUS       DASSWR010000606         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_361637,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000606 DASSWR010000000
VERSION     DASSWR010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361637"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1164
                     /locus_tag="VFO47_10080"
     CDS             4..1164
                     /locus_tag="VFO47_10080"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET8789083.1"
                     /translation="MTGVAVHFGAGHIGRGFVGLILHRAGYQVVFADVVDELIDALNT
                     TPSYLVKEVGLDSSEERVDNYRAINSRRDEPAVTDEIASADIVTTAVGPTVLKFVAPV
                     IAEGLRRRPAGAAPLAVMACENAINATDALKEHIRSAVPDAEWPAVAARAVFANTAVD
                     RIVPAQSPDAGLDVTVETYFEWAIERPPFGGHEPSIPDATWVDDLAPYIERKLFTVNT
                     GHATTAYHGFVRGIGKLSDALDDDVVRAAVEGVLSETKRLLVAKHGFSDEAQQAYVDK
                     ILQRFANPFLPDTVDRVGRQPLRKLSRTERLIAPAAELAERGVRPGHLLATVEAATRF
                     DVPDDPQSVELQQLLRTLPAAEATERITGLTPADPLYDDVVAVLARRVGVGS"
     gene            1176..3401
                     /locus_tag="VFO47_10085"
     CDS             1176..3401
                     /locus_tag="VFO47_10085"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET8789084.1"
                     /translation="MGPSSARLAVLTSGGDAQGMNPAVRAVVRTALNHGAEIFAVYEG
                     WQGLVDGGDHIRPVTWDDVSGIVNRGGTVIGTARSAAFRQREGRRRAARHLVQHGIDR
                     LVVIGGDGSLSGADLLRQEWPSLLEELLAEGAIDREAAERHPALMIAGLVGSIDNDML
                     GTDMTIGADSALHRIMEAMDAIGSTAESHQRSFVVEVMGRRCGYLALMSAIAGSAAYV
                     LIPEWPPDPGWEEDLCEIIESGRAAGRRHSIVVVAEGAHDSANQPITSEYVRQLLEER
                     LGGDVRLTTLGHVQRGGVPSAFDRWMSSILGHAAVEEVLAATPESVPQLIGLQDHRVA
                     KVPLMECVARTRELAERIEAKDYDTALLMRGDRYTEMIHVFRSISRALPSTARTRERA
                     SRIALLHVGGLAPGMNAVAGAAVRLGLDRGHTMLGVNGSFRGLMDGDVQELTWGDVEG
                     WTSLGGAELGISRYVPTVSDLYAIGRGLEDQRVDGLLIIGGWDAFEAACTMQRERARY
                     PAFRVPTIYLPATIDNNIPGSELSVGADSALGLIVDSIDRVRQAGIATRRCFVVETMG
                     GQCGYLALMGGLSGGAVRVYLHEEGITLQELAQDVERMVASFRVGQRLFLAVMNEKAS
                     PMYTSDFLCRLFNQESQGLFDAREVVLGQTQQGGAPSPFDRILGTRLAAHSIDWLSDQ
                     IESAGTGGAVIGLSQGKVRVAPLREAEELADWEQRRPLTQWWMGLRPLIDVLADRLAL
                     R"
     gene            complement(3532..4038)
                     /locus_tag="VFO47_10090"
     CDS             complement(3532..4038)
                     /locus_tag="VFO47_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8789085.1"
                     /translation="MADKLFPVVPDTGTMKAAQLSSEVTGDPGRWVRLDCLHVRHVQQ
                     AVEPGQALECPTCPPSSGGVLSSHTVLRRGPERRRRAWLDAGPQAPGKAGAFAAEAVT
                     LTGPFSLVDDAGRMASELVANALRHTDAPIELTVDAGDDVVRIEVRDDDATVPGTGKV
                     VSFELREV"
     gene            4271..>4355
                     /locus_tag="VFO47_10095"
     CDS             4271..>4355
                     /locus_tag="VFO47_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET8789086.1"
                     /translation="MDVDLDGRTAIVTGGASGIGEACARALA"
BASE COUNT          624 a         1551 c         1624 g          556 t
ORIGIN      
        1 agaatgaccg gcgtcgccgt ccacttcggc gccgggcaca tcggccgcgg gttcgtcggg
       61 ctgatcctgc accgggccgg ctaccaggtg gtgttcgccg acgtcgtcga cgagctcatc
      121 gacgccctca acaccacgcc gagctacctg gtcaaggagg tcgggctcga ctcgtccgag
      181 gagcgggtcg acaactaccg ggcgatcaac agccgccgcg acgagccggc cgtgaccgac
      241 gagatcgcgt cggccgacat cgtgaccacg gcggtcggcc cgacggtcct gaagttcgtc
      301 gcaccagtga tcgccgaagg gctgcggagg cggcccgccg gagccgcccc cctggccgtc
      361 atggcgtgcg agaacgccat caacgcgacc gacgcgctga aggagcacat ccgctcggcc
      421 gtgccggacg ccgagtggcc ggcggtggcg gccagggccg tcttcgccaa caccgcggtc
      481 gaccggatcg tgcccgccca gtcgccggac gccggcctgg acgtcaccgt cgagacctac
      541 ttcgagtggg ccatcgagcg gcccccgttc ggcggccatg agccgtcgat cccggacgcc
      601 acctgggttg acgacctggc gccgtacatc gagcggaagc tgttcaccgt caacaccggc
      661 cacgccacca ccgcctacca cggcttcgtc cggggcatcg gcaagctcag cgacgccctg
      721 gacgacgacg tggtccgggc cgccgtggag ggggtgctgt ccgagaccaa gcggctgctg
      781 gtggccaagc acgggttcag cgacgaggcc cagcaggcgt acgtcgacaa gatcctgcag
      841 cggttcgcga acccgttcct gccggacacc gtcgaccggg tcggccggca gccgctgcgc
      901 aagctgtcgc gcaccgagcg gctcatcgcc ccggccgccg agctggccga gcggggcgtc
      961 cggcccgggc acctgctggc cacggtggag gcggcgacgc ggttcgacgt ccccgacgac
     1021 ccgcagagcg ttgagctgca gcagctgctc aggaccctgc cggcggccga ggcgaccgag
     1081 cggatcaccg gcctgacgcc ggccgacccg ctgtacgacg acgtggtcgc cgtgctggcg
     1141 cgcagggtcg gcgtcgggag ctgagcacgg ccgccatggg cccgtcgagc gcacggctgg
     1201 ccgtgctgac cagtggtggc gacgcgcagg gcatgaaccc ggcggtgcgg gcggtggtcc
     1261 gcaccgcgct caaccacggc gccgagatct tcgccgtgta cgagggctgg caggggctgg
     1321 tcgacggcgg cgaccacatc cgcccggtga cctgggacga cgtctccggc atcgtcaacc
     1381 ggggcggcac cgtgatcggc accgcccgat cggccgcctt ccgccagcgc gagggccgcc
     1441 gccgcgccgc ccgccacctc gtccagcacg gcatcgaccg gctggtcgtc atcggcggcg
     1501 acggcagcct gtccggggcc gatctcctcc gccaggagtg gccgtcgctg ctggaggagc
     1561 tgctggccga gggcgccatc gaccgggagg cggccgagcg gcacccggcg ctgatgatcg
     1621 ccgggctggt cggctcgatc gacaacgaca tgctcggcac cgacatgacc atcggggccg
     1681 actcggccct gcaccggatc atggaggcga tggacgccat cggctcgacc gccgagagcc
     1741 accagcgcag cttcgtggtc gaggtgatgg gccggcgctg cggctacctg gccctgatga
     1801 gcgcgatcgc cgggagcgcc gcctacgtgc tgatcccgga gtggccgccc gaccccggct
     1861 gggaagagga cctgtgcgag atcatcgaga gcggccgcgc ggccggccgg cgccacagca
     1921 tcgtggtcgt cgccgagggc gcccacgact ccgccaacca gccgatcacc agtgagtacg
     1981 tgcgccagct gctggaggag cggctgggcg gggacgtccg gctgaccacc ctcgggcacg
     2041 tccagcgcgg cggcgtgccc agcgccttcg accggtggat gagctccatc ctcggccacg
     2101 cggccgtcga ggaggtgctc gcggccaccc cggagagcgt cccccagctg atcgggctgc
     2161 aggaccaccg tgtggccaag gtgccgctga tggagtgcgt cgcccggacg cgggagctgg
     2221 ccgagcggat cgaggccaag gactacgaca ccgccctgct gatgcgcggc gaccgctaca
     2281 ccgagatgat ccacgtcttc cgctccatct cccgggccct gcccagcacg gccaggacca
     2341 gggagcgggc cagccggatc gccctgctcc acgtgggcgg gctggccccg ggcatgaacg
     2401 ccgtcgccgg cgccgcggtc cggctcgggc tggaccgcgg gcacaccatg ctcggcgtca
     2461 acggcagctt ccggggcctg atggacgggg acgtgcagga gctgacctgg ggagacgtcg
     2521 agggctggac cagtctcggg ggcgccgagc tgggcatcag ccggtacgtg ccgaccgtca
     2581 gcgacctgta cgccatcggc cgcggcctgg aggaccagcg cgtcgacggg ctgctgatca
     2641 tcggcggctg ggacgcgttc gaggcggcct gcaccatgca gcgcgagcgc gcccgctacc
     2701 cggccttccg ggtgccgacg atctacctgc cggccacgat cgacaacaac atccccggct
     2761 cggagctgtc ggtgggcgcc gacagcgccc tcggcctgat cgtcgactcc atcgaccggg
     2821 tgcggcaggc cggcatcgcc acccgccgct gcttcgtggt cgagacgatg ggcggccagt
     2881 gcggctacct ggccctgatg ggcgggctgt ccgggggcgc ggtccgcgtc tacctgcacg
     2941 aggagggcat caccctccag gagctggccc aggacgtcga gcggatggtg gcgagcttcc
     3001 gggtcggcca gcggctcttc ctcgccgtca tgaacgagaa ggccagcccc atgtacacca
     3061 gcgacttcct gtgccggctg ttcaaccagg agagccaggg gctgttcgac gcccgcgagg
     3121 tggtcctggg ccagacccag cagggcggcg cgccgtcccc gttcgaccgg atcctcggca
     3181 cccggctggc cgcgcactcg atcgactggc tcagcgacca gatcgagagc gccggcaccg
     3241 gcggggcggt gatcgggctg tcccagggca aggtccgggt ggccccgctg cgggaggccg
     3301 aggagctggc cgactgggag cagcggcgcc cgctgacgca gtggtggatg gggctgcggc
     3361 cgctgatcga cgtgctggcc gaccggctcg ccctccgtta gaacagcggt cgaggatccg
     3421 gcgccgcatg cagtgctgac ccatccgggc agcgctggag cttgcgcgcc gcccccggcg
     3481 gccacccaat tccctcaaac ccggatactc gagcggtgtt ccaggcctcc gtcagacctc
     3541 gcggagctcg aaggagacga ccttgccggt cccggggacg gtggcgtcgt cgtcgcggac
     3601 ctcgatccgg accacgtcgt cgccggcgtc gacggtcagc tcgatcgggg cgtcggtgtg
     3661 gcggagggcg ttggccacca gctcgctggc catccggccg gcgtcgtcga ccaggctgaa
     3721 ggggccggtc agggtcaccg cctcggcggc gaacgcgccc gccttgcccg gggcctgcgg
     3781 gccggcgtcc agccaggccc ggcggcggcg ctccgggccc cggcgcagca ccgtgtggga
     3841 ggagaggacg ccgcccgacg acggcgggca ggtcgggcac tccagcgcct gcccgggctc
     3901 gaccgcctgc tggacgtggc gcacgtgcag gcagtcgagg cgcacccagc ggccggggtc
     3961 cccggtgacc tcggacgaca actgggccgc cttcatggtt ccggtgtcgg gcacgacggg
     4021 gaagagcttg tcagccattg gcgatccccc aggggaagtg gcggtacggc ggccatggcg
     4081 ggaagtgcct gacgggtacc atacggccgg atcggggccc gggagaagac ctgatcgggg
     4141 gattcggggt gccggaaaca aagagttcac aagcatcgat ccacggaggg ctaggcttga
     4201 gccccggctg gcctcggcaa ggtcatgagg cacgagccgc ggccgtgagc gctctggagg
     4261 tggtggggtg atggacgtcg acctggacgg caggaccgcc atcgtcaccg gcggggccag
     4321 cggcatcggc gaggcctgcg cccgcgccct ggccg
//
LOCUS       DASSWR010000607         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_277220,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000607 DASSWR010000000
VERSION     DASSWR010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277220"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFO47_10100"
     CDS             <1..653
                     /locus_tag="VFO47_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HET8789087.1"
                     /translation="DPSVNTAYGVLKGMQAVAEELWKTHDLAGRHVVVQGVGKVGTHL
                     CHLLHQAGARLTVADVDVDNVARAVREFGADTVEPGKAHAVACDIFAPCALGAVVNDD
                     TLPELKCAAIAGSANNVLARDEHGYALRQLGILYAPDYVVNAGGLINVADELQGYSPE
                     RAKAKVEGVFGSVRDIFHRAKAEGIPTSEAADRIAKDRMRDISRLRLIRVLPGVSG"
     gene            complement(643..1686)
                     /gene="purM"
                     /locus_tag="VFO47_10105"
     CDS             complement(643..1686)
                     /gene="purM"
                     /locus_tag="VFO47_10105"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET8789088.1"
                     /translation="MDREPLTYAQAGVDVEAGDRAVALMRAAVEATHGPRVLGGIGGF
                     AGLWRLAGSSGRAGAAGLPGDPVLAAASDGVGTKLLVAQALDRHDTVGIDLVAMVVDD
                     VVVTGAEPLFVLDYLAVGRVDPERVAAIVSGVAEGCRLARCALLGGETAEHPDAMAPD
                     AYDLAGFAVGMVERDRLLGPERVAAGDVLLGLAASGLHANGFSLARRVLARAGVGYDQ
                     RLPELGVPVGEALLTPTRIYAPHLVDLLAAGVEVHALCHVTGGGLPGNLPRCLPPGLT
                     ARVDRASWQVPSLFGLLARLGPVADEELARATNLGVGMVVVLPPGEADRAVAFLADRE
                     VPAWVMGEVVASR"
     gene            complement(1687..>2028)
                     /locus_tag="VFO47_10110"
     CDS             complement(1687..>2028)
                     /locus_tag="VFO47_10110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544228.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET8789089.1"
                     /translation="TTKAIVAMLREAGAREVHVRITSPPVRWPCFYGIDMPTRAELVA
                     SDLLSDEVASYVGADSLGYLSLEALTETAADPGEGPGGLCTACVTGSYPIPVESQVQG
                     KELLEGLAPRR"
BASE COUNT          278 a          757 c          772 g          221 t
ORIGIN      
        1 gcgacccgtc ggtcaacacc gcctacgggg tgctgaaggg catgcaggcg gtggccgagg
       61 agctgtggaa gacgcacgac ctggccggcc gccacgtcgt ggtccagggc gtcggcaagg
      121 tcggcaccca cctctgccac ctgctgcacc aggccggcgc ccggctgacc gtggccgacg
      181 tggacgtcga caacgtggcc cgggcggtca gggagttcgg cgccgacacc gtggagccgg
      241 gcaaggccca cgcggtcgcg tgcgacatct tcgccccctg cgccctgggg gcggtcgtca
      301 acgacgacac cctgcccgag ctgaagtgcg ccgccatcgc cgggtcggcc aacaacgtgc
      361 tcgcccgcga cgagcacggc tacgccctgc gccagctcgg catcctgtac gcgcccgact
      421 acgtggtcaa cgccggcggc ctgatcaacg tggccgacga gctccagggc tactcccccg
      481 agcgggccaa ggccaaggtc gagggcgtgt tcggctccgt ccgggacatc ttccatcgcg
      541 ccaaggccga ggggatcccg accagcgagg ccgccgaccg catcgccaag gaccgcatgc
      601 gcgacatctc ccgcctccgc ctgatccggg tcctcccggg ggtcagcggc tagccaccac
      661 ctcgcccatc acccaggcgg ggacctcgcg gtcggcgagg aaggcgacgg cccggtcggc
      721 ctcgccgggc gggaggacga ccaccatccc cacgccgagg ttggtggccc gggccagctc
      781 ctcgtcggcg acggggccga ggcgggccag cagcccgaac agggacggca cctgccagga
      841 ggcgcggtcg accctggccg tcaggccggg gggaaggcag cgggggaggt tgccggggag
      901 gccgccgccg gtgacgtggc agagggcgtg gacctcgacc ccggcggcga gcaggtcgac
      961 caggtggggg gcgtagatgc gggtcggggt gagcagcgcc tccccgaccg ggacgccgag
     1021 ctcgggcagg cgctggtcgt agccgacccc ggccctggcc aggacccggc gggccaggct
     1081 gaagccgttg gcgtgcagcc ccgaggcggc gagcccgagg agcacgtcgc cggcggcgac
     1141 ccgttcgggg ccgagcagcc ggtcgcgctc caccatcccg acggcgaacc cggcgaggtc
     1201 gtaggcgtcc ggggccatcg cgtcggggtg ctcggccgtc tccccgccga gcagggcgca
     1261 gcgggccagc cggcagccct cggcgacccc ggagacgatg gcggccaccc gctccgggtc
     1321 gacccggccc acggccaggt agtcgaggac gaacagcggc tcggccccgg tcacgaccac
     1381 gtcgtcgacc accatggcga ccaggtcgat gccgaccgtg tcgtggcggt cgagggcctg
     1441 ggcgaccagc agcttggtgc cgacgccgtc gctggccgcg gccaggaccg ggtcgccggg
     1501 caggccggcc gcgccggccc ggccggagga accggccagg cgccacagcc ccgcgaaccc
     1561 gccgatgccg cccagcaccc tgggcccgtg ggtcgcctcc accgcggccc gcatcagggc
     1621 cacggcgcgg tcaccggcct cgacgtcgac cccggcctgg gcgtaggtga gcggctcccg
     1681 gtccatctat ctccggggcg ccaggccctc gagcagctcc ttgccctgga cctgggactc
     1741 caccgggatc gggtagctcc cggtgacgca ggccgtgcag agcccgcccg gcccctcgcc
     1801 ggggtcggcc gcggtctcgg tgagggcctc aagggacagg tagccgagcg agtcggcgcc
     1861 gacgtagctg gccacctcgt cgctgagcag gtcgctggcg accagctcgg cccgggtggg
     1921 catgtcgatg ccgtagaagc acggccagcg caccgggggc gaggtgatgc ggacgtggac
     1981 ctcgcgggcc ccggcctccc ggagcatggc gacgatcgcc ttggtggt
//
LOCUS       DASSWR010000608         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_305508,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000608 DASSWR010000000
VERSION     DASSWR010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305508"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1507)
                     /locus_tag="VFO47_10115"
     CDS             complement(260..1507)
                     /locus_tag="VFO47_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789090.1"
                     /translation="MTQLHDDVNGLGDDWRPGRDPAPPAEGHGRPRLVIAGGRDVADQ
                     NAPGQSDDSQWNSDQWDAGDPWDSGQWDASQLDSSGQWDARDPWDRDGLDATQAAPAG
                     GEPLFEQLRSAYLHRDDTNDWARDTDTGGWAADDAGRWEAGVATDLDDDRYRWVDSPA
                     AWESGSEADWDDDADEFDEMDEEEEFDPEPRARGSRRSRSSAGRAGLRRTSTLLNATT
                     VTLFVVGAIAAAGLTFWILSARLLTTRQLFFLTHSTFGIIIVHAFGGGLGTLLTSGDS
                     RMKDRIRKLSTASLAVVAWLASAIGTWFGYAGYRAKVPPGGDITMYPREYLLNSPQLA
                     FWETFAMEWKVHIGWTTPFLATAVAFTALRYRRRLVADRLVRKVMTIMLLLAFAGALV
                     AAGLGAVVNVTAPNDFMHRGWSP"
     gene            complement(2254..2703)
                     /locus_tag="VFO47_10120"
     CDS             complement(2254..2703)
                     /locus_tag="VFO47_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET8789091.1"
                     /translation="MSRRAEESGQALVLLVGLVVLVLMVLALGWDTSNWFLGHRALGN
                     LADGAAVAAANDVDTRTWYLSGGRSVRVAQERARATVTAYLAESVGDSGITGVRLRSV
                     KVVPGEGGPEVTVRLAAPARVGLLRLLRLVPPEMEGGAAATARLVPP"
     gene            complement(2700..3149)
                     /locus_tag="VFO47_10125"
     CDS             complement(2700..3149)
                     /locus_tag="VFO47_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789092.1"
                     /translation="MELLVVFLTLLVPLVYVMVVMADVQRALLATSSAAREAGRVYAT
                     GSDRLDAERRAALAYREVLATYGMRPGDPGAGMRLRAACPAGDGSGPGVGSGCAGGFG
                     PGAEVEVVVTYRVPVARVPFLGAVAGPGVTVGATHHTRVDRYRGLGG"
BASE COUNT          494 a         1358 c         1083 g          430 t
ORIGIN      
        1 cgcgcggcga accggccggc ccgctcgacc caaatcgccc acccggcggg tgccgcatgc
       61 cgcgaccccc gggtcgcccg gatgctctcg gtccgcccag cccgggtcgc ccggcgggcc
      121 ggggtggcct gacctccgct cacctcgacc cggtgggggc gccgctcgtg ccggccgacc
      181 gtctgtcctt gatgtgccgc cacggcagcg cggcctgctc gatcgcgcat cagcgcatca
      241 ccgtctctca tcgcggccgt cagggactcc agccccggtg catgaagtcg ttgggagcgg
      301 tcacgttgac caccgccccc aggcccgctg cgaccagcgc tccggcgaag gcgagcagca
      361 gcatgatcgt catgaccttg cggaccagcc ggtcggcgac cagccggcgc cggtagcgca
      421 gcgccgtgaa ggccaccgcc gtcgccagga agggcgtggt ccagccgatg tggaccttcc
      481 actccatggc gaaggtctcc cagaacgcga gctgcggact gttcagcagg tactcgcgcg
      541 ggtacatggt gatgtcgccg cccgggggca ccttggcccg gtagccggcg tacccgaacc
      601 aggtcccgat ggccgaggcc agccaggcca ccaccgccag cgaggccgtc gagagcttgc
      661 ggatccggtc cttcatccgc gagtccccgc tggtcagcag cgtccccaac ccgccaccga
      721 aggcgtggac gatgatgatc ccgaaggtcg agtgggtgag gaagaacagc tggcgggtgg
      781 tcagcagccg cgccgagagg atccagaacg tgagcccggc cgcggcgatg gccccgacca
      841 cgaacagcgt gacggtggtg gcgttcagca gcgtgctcgt gcgccgcagc cccgcccggc
      901 ccgccgagga gcgcgaccgc cgcgacccgc gggcccgcgg ctccgggtcg aactcctctt
      961 cctcgtccat ctcgtcgaac tcgtcggcgt cgtcgtccca gtcggcctcg gagcccgact
     1021 cccacgccgc cggcgagtcc acccagcggt agcggtcgtc gtcgaggtcg gtcgcgaccc
     1081 cggcctccca gcggccggcg tcgtcggcgg cccagccgcc ggtgtcggtg tcgcgcgccc
     1141 agtcgttggt gtcgtcgcgg tggaggtagg cgctgcggag ctgctcgaac agcggctccc
     1201 cgccggccgg cgccgcctgg gtggcgtcca ggccgtcacg gtcccagggg tcgcgggcgt
     1261 cccattggcc actggaatcc agctggctgg catcccactg accgctgtcc caagggtcgc
     1321 cggcgtccca ctggtcggag ttccactggg agtcatccga ctggccggga gcgttctggt
     1381 cggcgacgtc ccgcccgccg gcgatcacca gccgaggccg gccgtggccc tccgccggtg
     1441 gcgccgggtc ccgtccaggg cgccagtcgt cgcccaggcc gttgacgtcg tcgtggagct
     1501 gcgtcacgag ggccccccct ccatagcagt ggtcacgggc acgcaagcag cgcggaagcc
     1561 ttcgggaagg cacagggaga accaggccgc cattgcagcc acctcatttg tttcatagcg
     1621 cggccaaccg gccacgcggc cgacgggggg atcacgaacc agccgggaaa ttcctagcca
     1681 ttcctggcga gccgtgcgtc agtcttgcga ctcgtaattt cacaagcccg cggtgagcgc
     1741 tggggggagc gcctcgagca gtccctgtcg tgggggcgac aggaaagggg aagctcggac
     1801 cgatccgcga acggcgaaat cagagcggtg cttcttgatg tcgctgagcc gggggggagg
     1861 ctctttggtg cagtgccatg ggggtagcgc gtcggactgt acggcaccac cctgttggag
     1921 tcaaatcgta tacacgtcga gatcatcctg tgatctacgt agatgtctcc ctgcagatcg
     1981 tgggaatccg ccggacatcc ggggagttcc cccacaaact acagcctgca accgttctcc
     2041 gccggctcca ccatccaggc gagccggccc gaccgcggac ccgggaagga ggtgatccct
     2101 ggcacctgcc atgggagacg cccggtccct tgaccgaggc gttagcgcgt agcgaacgag
     2161 cggccgggcg gaggaccctc ccccgcccgg ccgctcgatt catgcccagc ccgggtcgag
     2221 ccccgctccc cgccggaact ccgggccctt cgctcaagga ggaaccagac gcgccgtggc
     2281 cgccgcgcca ccctccatct ccggagggac cagccgcagc agccggagca atcccacccg
     2341 cgccggcgcc gcgagccgaa ccgtcacctc cggcccgccc tctcccggaa ccaccttcac
     2401 cgaccgcaac cgcaccccgg tgatcccgga gtcccccacc gactccgcca gataggccgt
     2461 caccgtcgcc cgcgcccgct cctgcgcaac ccggacgctc cgccccccac tcaggtacca
     2521 ggtcctggtg tcgacgtcgt tcgcggccgc caccgccgcc ccgtccgcca ggttccccaa
     2581 cgcccgatgc cccaggaacc agttcgacgt gtcccatccc aacgccagca ccatcagcac
     2641 cagcaccacc aacccgacca gcagcaccag cgcctgcccg ctctcctccg cccgccggct
     2701 catccgccca gcccccggta ccggtcgacc cgggtgtggt gcgtggcccc gaccgtcacc
     2761 cccggcccgg caacagcacc aaggaacggc acccgagcca ccggcacccg ataagtcacc
     2821 accacctcca cctccgcccc gggcccgaac ccacctgcac accccgaccc caccccaggc
     2881 ccggatccat ccccggccgg gcaggcagcc cgcagtcgca tcccggcgcc ggggtccccg
     2941 gggcgcatcc cgtaggtggc cagcacctcc cggtacgcca gcgccgcccg ccgctccgcg
     3001 tccagccggt ccgacccggt cgcgtacacc cgaccggcct cccgcgccgc gctggaggtg
     3061 gccagcaacg cccgctggac gtcggccatc accaccatca cgtacaccag cggcaccagc
     3121 agggtcagga acaccaccag cagctccacc accgccgccc cccgctccgc tttcgccgtc
     3181 cccgctcccg ccgcgtccgc tcccgccgga cccaggccgg acccgaacgg cgggccgcga
     3241 accgcaccca ccgtcgccgg ctcggccgcc gcccggccct cgggtccccg acgggcccgg
     3301 cgcagtcggt cgcgtggcgg atctcgttgt cgccgctgcg cacgtccggc accggtcaca
     3361 gcgac
//
LOCUS       DASSWR010000609         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_20969,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000609 DASSWR010000000
VERSION     DASSWR010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20969"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFO47_10130"
     CDS             <1..905
                     /locus_tag="VFO47_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8789093.1"
                     /translation="LDLEQLLAALADALASYSAGRASVPPRVAASATDGILGAMPVHL
                     PGVALGAKLVSVFPANHERGRPSHQALIALFDPDDGTPVAVMDGTRITAARTGAAAAV
                     AAGALARPDAAVLAILGAGVQGRSHLEAFPLVREFREIRVASRTREHADTLAAGHPVA
                     RAVGSFEEAVAGADVVCCCTDAREPVLEATWLAPGTHVSSVGGTFGPELDPATMHAGR
                     VFVEWRGAAANPPPAGAHELQGLDPATLTEVGEVLAGTRPGRTSPDELTIYKSTGHAV
                     EDAAAADLVYRRALESGAGVTVSI"
     gene            complement(909..2984)
                     /gene="lpdA"
                     /locus_tag="VFO47_10135"
     CDS             complement(909..2984)
                     /gene="lpdA"
                     /locus_tag="VFO47_10135"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET8789094.1"
                     /translation="MEIRIPDIGDFADVPVIEVHVSPGSVVAAEDPLVTLESDKATMD
                     VPSPVAGTIAEVRIAVGDQVSEGDVVATLEGGEQPAAPAKERVHESAHPEAPEPAGYG
                     SPAGVYERLEVRVPDLGDFENVPVIEVLVTPGDRISAEDPLLTLESDKATMDVPAPAG
                     GTVAEVRVAVGDTVSEGDVIVELQTGQEAAPAHADPVAAHTAPAQVQTAPVASASAAG
                     IEADVQADVLVLGAGPGGYSAAFRAADLGKSVVLVDRSPILGGVCLNIGCIPSKALLH
                     AARVIAETKEMAEHGLSFGEPAVDLDKLRGWKESVVKRLTNGLAGLAKQRKVKVVTGF
                     GTFASPNHLEVRADDGSTTMVGFEQAIIAAGSEPVTLPFIPHDDPRVIDSTGALELAS
                     VPERLLVVGGGIIGLEMATVYSELGAKVTIVELLDQLIPGADKDLVNPLFKRISKRYE
                     AIYLTTKLTQVDATADGLVVHFEGAKAPETATFDQVLVAVGRRPNGRAVGAEKAGVAV
                     DERGFIGVDKQLRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEVAAGKPSYFDVRGI
                     PSVAYTDPEVAWVGVTETEAKAAGVKYGKGTFPWAASGRSLSLGRDEGMTKLLFDEAT
                     HRLIGAAAVGPNAGELIAETALAVEMGADATDIALTVHPHPTLSETVGMSAEAFEGTI
                     TDLYLPKRK"
     gene            complement(2998..>3985)
                     /locus_tag="VFO47_10140"
     CDS             complement(2998..>3985)
                     /locus_tag="VFO47_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET8789095.1"
                     /translation="APAPAVQAPPAPAAAPAAGPAGAVADFTGAHAGPSVRRLARELG
                     VDLNTVNGTGEKNRITKDDLLAAVRGPAQPAPAAAAPAGAGIPEIPAQDFSKFGPVET
                     VPLPRIKKLSGPFLHRSWLNVPHVTHNDEADITELDPYRRELDTAAKAEGYRVTLLAF
                     LMKAAVSALRQFPEVNSSLSPEKDALILKSYYHIGIAVDTPGGLVVPVIHDVDRKGVT
                     ALSKELGEVSKRARDGKLSPDDMSGGSFTISSLGGIGGTSFTPIVNAPEVAILGVVRS
                     RMSPVWNGSEFVPRMMLPLSLSYDHRVIDGALAARFTRTLATVLEDVRRLLL"
BASE COUNT          489 a         1423 c         1515 g          558 t
ORIGIN      
        1 tgctcgacct cgagcagctg ctcgccgccc tcgccgacgc cctggccagc tacagcgccg
       61 ggcgggcgtc cgtgccgccc cgggtggcgg cgtcggccac cgacgggatc ctcggggcca
      121 tgccggtgca cctgcccggg gtcgccctcg gcgccaagct ggtcagcgtc ttccccgcca
      181 accacgagcg gggccgcccc tcccaccagg cgctgatcgc gctgttcgac ccggacgacg
      241 gcaccccggt cgcggtcatg gacggcaccc gcatcaccgc cgcacgcacc ggcgccgcgg
      301 ccgcggtggc ggccggcgcc ctggcccggc ccgacgcggc cgtcctggcc atcctcggcg
      361 cgggcgtcca gggccgctcc cacctggagg cgttcccgct ggtccgcgag ttccgcgaga
      421 tccgggtggc ctcgcggacc cgcgagcacg ccgacaccct cgccgcgggc cacccggtgg
      481 cccgggcggt tggctcgttc gaggaggcgg tcgccggggc cgacgtggtc tgctgctgca
      541 ccgacgctcg cgagcccgtc ctggaggcga cctggctggc ccctggcacc cacgtcagct
      601 cggtcggcgg caccttcggc cccgagctcg acccggcgac catgcatgcc ggccgggtct
      661 tcgtcgagtg gcgcggcgcc gccgccaacc cgcccccggc cggcgcccac gagctccagg
      721 gcctcgaccc cgccaccctc accgaggtcg gcgaggtcct cgccggcacc cgccccggcc
      781 gcacctcccc cgacgagctc accatctaca agtcgaccgg ccatgccgtc gaggacgccg
      841 ccgccgccga cctggtctac cgccgcgccc tggagtcggg cgcgggcgtg acggtctcga
      901 tctgaccgct acttccgctt gggcaggtag aggtcggtga tggtgccctc gaacgcctcg
      961 gcggacatgc cgacggtctc cgagagggtg gggtgggggt ggacggtgag ggcgatgtcg
     1021 gtggcgtcgg cgcccatctc gacggccagg gcggtctcgg cgatcagctc gccggcgttg
     1081 gggccgacgg cggcggcgcc gatcaggcgg tgggtggcct cgtcgaacag gagcttggtc
     1141 atgccctcgt cgcggcccag ggacagggaa cggccgctgg ccgcccacgg gaaggtgccc
     1201 ttgccgtact tgacgccggc ggccttggcc tcggtctcgg tgacgccgac ccaggcgacc
     1261 tcggggtcgg tgtaggcgac cgaggggatg ccgcggacgt cgaagtagct cggcttgccg
     1321 gcggcgacct cggccgcgac gtggccctcg tgcaccgcct tgtgggccag catcggctgg
     1381 ccgacgacgt cgccgatggc gaagatgtgg ggcacgttgg tgcgcagctg cttgtccacg
     1441 ccgatgaagc cgcgctcgtc aacggccacg ccggccttct cggccccgac cgcgcggccg
     1501 ttggggcggc ggccgacggc caccaggacc tggtcgaagg tggcggtctc gggcgccttg
     1561 gccccctcga agtggacgac cagcccgtcg gcggtggcgt cgacctgggt gagcttggtg
     1621 gtcaggtaga tggcctcgta ccgcttggag atccgcttga acagcgggtt gaccagatcc
     1681 ttatcggcgc ccgggatgag ctggtcgagc agctcgacga tggtgacctt ggcccccagc
     1741 tcggagtaga cggtggccat ctccaggccg atgatcccgc cgccgacgac cagcagccgc
     1801 tcggggacgc tggccagctc cagggccccg gtggagtcga tcacccgtgg gtcgtcgtgg
     1861 gggatgaacg gcagggtcac cggctccgag ccggctgcga tgatcgcctg ctcgaacccc
     1921 accatcgtcg tggagccgtc gtcggcacgg acctccagat ggtttgggga ggcgaaggtg
     1981 ccgaagccgg tgaccacctt gaccttgcgc tgcttggcca ggccggccag gccgttcgtg
     2041 agccgcttga cgaccgactc cttccagccc cggagcttgt ccaggtcgac ggccggctcg
     2101 ccgaaggaca ggccgtgctc ggccatctcc ttggtctcgg cgatcacccg ggccgcgtgc
     2161 agcagggcct tggaggggat gcagccgatg ttgagacaga ccccgccgag gatcggggag
     2221 cggtccacca gcacgaccga cttgccgagg tcggcggccc ggaaggcggc gctgtagccg
     2281 ccgggcccgg ccccgagcac cagcacgtcg gcctggacgt cggcctcgat gccggccgcc
     2341 gaggcggagg cgaccggggc cgtctggacc tgggccgggg cggtgtgggc ggcgaccggg
     2401 tccgcgtggg ccggggccgc ctcctggccg gtctggagct cgacgatcac gtcgccctcg
     2461 gacaccgtgt cgccgacggc gacccggacc tcggccacgg tcccgccggc gggggcgggg
     2521 acgtccatgg tcgccttgtc ggactccagg gtgagtagcg ggtcctcggc cgagatcctg
     2581 tcgccagggg tgacgagaac ctcgatcacg ggcacgttct cgaagtcgcc gaggtcgggc
     2641 acccggacct cgaggcgctc gtagaccccg gccggcgacc cgtagccggc cggctcgggc
     2701 gcctccgggt gggcgctctc gtgcacccgc tccttcgctg gggccgccgg ctgctcccct
     2761 ccttcgagcg tggccaccac gtcgccctcc gagacctggt cgccgaccgc gatgcggacc
     2821 tcggcgatgg tgccggccac cggcgacggc acgtccatgg ttgccttgtc ggactccagc
     2881 gtgaccagcg ggtcctcggc cgccacgacg ctgcccgggc tgacgtgcac ctcgatcacg
     2941 ggcacgtcgg cgaagtcgcc gatgtccggg atacggatct ccatgggtcc tccccgacta
     3001 gagcagcagg cggcggacgt cctcgagcac ggtggccagg gtccgggtga accgggcggc
     3061 cagggcgccg tcgatcaccc ggtggtcgta ggagagcgac agcggcagca tcatgcgggg
     3121 aacgaactcc gagccgttcc agaccggcga catcctggag cggacgacgc cgaggatggc
     3181 gacctcgggg gcgttgacga tcggggtgaa gctggtcccc ccgatgccgc cgaggctgga
     3241 gatggtgaag ctgcccccgg acatgtcgtc gggcgacagc ttgccgtcgc gggcccgctt
     3301 ggagacctcg cccagctcct tgctcagggc ggtgacgccc ttgcggtcga catcgtggat
     3361 gaccggcacg accaggccgc cgggcgtgtc gacggcaatc ccgatgtggt agtagctctt
     3421 gaggatcagc gcgtccttct cgggcgacag cgagctgttg acctcgggga actggcgcag
     3481 ggcggacacc gcggccttca tcaggaaggc gagcagcgtg acccggtagc cctcggcctt
     3541 ggcggcggtg tcgagctcgc gccggtaggg gtcgagctcg gtgatgtcgg cctcgtcgtt
     3601 gtgggtgaca tgggggacgt tcagccacga ccggtgcagg aacggcccgg acagcttctt
     3661 gatccggggc agcgggaccg tctcgacggg accgaacttg gagaagtcct gggccgggat
     3721 ctcggggatg ccggcgccgg ccggggcggc ggcggccggg gccggctggg ccgggccgcg
     3781 gacggcggcg agcaggtcgt ccttggtgat ccggttcttc tcgccggtgc cgttgacggt
     3841 gttgaggtcc acgccgagct cccgggccag ccggcgcacg ctggggccgg cgtgggcgcc
     3901 ggtgaagtcg gcgacggcgc cggcggggcc ggccgccggg gcggccgccg gcgccggggg
     3961 cgcctggacc gccggggcgg gcgcg
//
LOCUS       DASSWR010000610         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_51342,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000610 DASSWR010000000
VERSION     DASSWR010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51342"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFO47_10145"
     CDS             <1..701
                     /locus_tag="VFO47_10145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET8789096.1"
                     /translation="WMGNPGVGSLHAIRRRVEHHDPAPLELEEYSHFGMVGRYAAGAA
                     NLPFWPLRSYFETDLPKVNPNIRPVTSPFGGEPVFAVPPLNPDVAVVHAQRADAAGNT
                     QIWGLTGCQKEAAFAASRVIVVVEELVAEDVVRADPNRTLIPGIKVDAVVVCPRGAHP
                     SYAQGYYDRDNRFYLEWDKISRDPEALEGWLDEWVHGTATHEEYVEKLGTERWAELTP
                     APALSGSVDYGDYR"
     gene            701..1468
                     /locus_tag="VFO47_10150"
     CDS             701..1468
                     /locus_tag="VFO47_10150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET8789097.1"
                     /translation="MGDFSKNEMLVVAGARALAGKRVCFVGIGLPNIAVALAQRTVAP
                     AIELIYESGVYGARPARLPLSIGDPCLVTGATAAMSMVELFSYYLQGGLVDVGFLGAA
                     QLDRFGNINTTVIGGYDRPKVRLPGSGGACEIAINAREVFVLMRQSARSFVERIDFRT
                     SPGNLGGAALRAAQGWQGRGPSVVVTDLGRYGFDPDTGEMVLEAVHPGVTEADVRAAT
                     GWDLRVAGDLEVTPDPTDEELRLIREELDPDGVYVKG"
     gene            1465..>2593
                     /locus_tag="VFO47_10155"
     CDS             1465..>2593
                     /locus_tag="VFO47_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8789098.1"
                     /translation="MSHVFARGTGLPTVARAEGCQLWDTEGRRYLDGAGGAIVVGIGH
                     GDASVAEAIAGQAARVAYAHGTMFASEELEAYAGELAPLLPVDDPRVYPVSGGSEAIE
                     TALKMARAYHLARGEDRARVIGRRGSYHGNTRGALDVGGREPLRRPYLPWLGLADHTL
                     AAYEYRCPFPGTHPAGCGARYADALEREILASPPGTVAAFVAEPVAGAGLGAAVPPDD
                     YWPAVTEVCRRHGVLVIADEVMTGFGRTGRWFACDHWGVRPDILVLAKGAASGYWPLG
                     LAVASGAVHDTIGEGGLTHGLTYSHHVVGAAAGRAVLRVLRECRLVEAAETQGKRLRA
                     GLEARLGGHPAAGDIRGLGLMVAVELVADRPTRTPFPRPARV"
BASE COUNT          329 a          977 c          959 g          328 t
ORIGIN      
        1 cctggatggg gaacccgggg gtggggtcgc tgcacgccat ccgccgccgg gtcgagcacc
       61 acgacccggc tccgctggag ctggaggagt acagccactt cgggatggtc gggcgctacg
      121 ccgccggggc cgccaacctg cccttctggc ccctgcggtc ctacttcgag accgacctgc
      181 cgaaggtgaa ccccaacatc cgcccggtca cctccccgtt cgggggcgag ccggtgttcg
      241 cggtgccacc cctgaacccg gacgtggccg tcgtccacgc ccagcgggcc gacgccgccg
      301 ggaacaccca gatctggggc ctgaccggct gccagaagga ggcggcgttc gcggccagcc
      361 gggtgatcgt ggtcgtcgag gagctggtgg ccgaggacgt cgtgcgggcc gaccccaacc
      421 ggaccctgat cccggggatc aaggtggacg cggtggtggt ctgcccgcgc ggcgcccacc
      481 cctcctacgc ccagggctac tacgaccgcg acaaccgctt ctacctggag tgggacaaga
      541 tcagccgcga ccccgaagcg ctcgaaggat ggctggacga gtgggtccac gggacggcga
      601 cccacgagga gtacgtggag aagctcggga cggagcgatg ggccgagctg accccggcgc
      661 cggccctttc cgggtcggtc gactacgggg actaccgctg atgggcgact tctccaagaa
      721 cgagatgctg gtggtggccg gggcgcgggc cctggccggc aagcgggtct gcttcgtcgg
      781 catcggcctg cccaacatcg ccgtcgccct ggcccagcgg accgtcgccc cggcgatcga
      841 gctgatctac gagtcggggg tgtacggggc ccggccggcc cggctgcccc tctccatcgg
      901 cgacccgtgc ctggtcacgg gggcgacggc cgccatgagc atggtcgagc tgttcagcta
      961 ctacctgcag ggcgggctgg tcgacgtcgg cttcctcggc gccgcccagc tcgaccggtt
     1021 cggcaacatc aacaccaccg tgatcggcgg ctacgaccgg ccgaaggtgc gcctgcccgg
     1081 gtccggaggg gcatgcgaga tcgccatcaa cgcccgcgag gtgttcgtgc tcatgcgcca
     1141 gtcggcccgc tcgttcgtgg agcggatcga cttccggacc agcccgggca acctcggcgg
     1201 ggccgccctg cgggccgccc agggctggca gggccggggc ccgtcggtgg tcgtcaccga
     1261 cctcggccgc tacggcttcg accccgacac cggcgagatg gtcctggagg ccgtccaccc
     1321 cggcgtcacc gaggccgacg tccgcgccgc caccggctgg gacctccggg tcgccggcga
     1381 cctcgaggtc acgcccgacc ccaccgacga ggagctgcgg ctgatccgcg aggagctcga
     1441 ccccgacggg gtgtacgtca agggatgagc cacgtcttcg cgagagggac cggcctgccg
     1501 acggtggcca gggccgaagg ctgccagctg tgggacaccg aggggcggcg ctacctggac
     1561 ggggccggcg gggcgatcgt ggtcgggatc ggccacggcg acgcctcggt ggccgaggcc
     1621 atcgccgggc aggcggccag ggtcgcctac gcccacggca ccatgttcgc ctccgaggag
     1681 ctggaggcct acgccggcga gctggccccg ctgctgccgg tggacgaccc gcgcgtctat
     1741 ccggtgtcgg ggggcagcga ggcgatcgag accgccctga agatggcccg cgcctaccac
     1801 ctggcccggg gcgaggaccg cgcccgggtg atcggccgaa ggggcagcta ccacggcaac
     1861 acccgggggg cgctggacgt gggcggccgc gagccgctgc gccgccccta cctgccctgg
     1921 ctcggcctgg ccgaccacac cctggccgcc tacgagtacc gctgcccgtt ccccggcacc
     1981 cacccggccg gctgcggggc ccgctacgcc gacgccctgg agcgggagat cctggcctcg
     2041 cccccgggca cggtggccgc gttcgtggcc gagccggtcg ccggggccgg ccttggcgcg
     2101 gccgtcccgc ccgacgacta ctggccggcg gtgaccgagg tctgccgccg ccacggggtg
     2161 ctggtcatcg ccgacgaggt catgaccggg ttcgggcgca ccggccgctg gttcgcctgc
     2221 gaccactggg gggtccggcc cgacatcctc gtcctggcca agggggccgc ctccggctac
     2281 tggccgctcg ggctggccgt cgcctccggg gccgtccacg acaccatcgg cgagggcggc
     2341 ctcacccacg gcctcaccta ctcccaccac gtggtcgggg ccgccgccgg ccgggccgtg
     2401 ctccgggtgc tgcgcgagtg tcgcctggtc gaggccgccg agacccaggg caagcgcctc
     2461 cgggccggcc tcgaggcccg cctgggcggc cacccggccg ccggcgacat ccgcggcctt
     2521 ggcctgatgg tcgccgtcga gctggtcgcc gaccgcccca cccggacccc gttcccccgg
     2581 ccagcgcggg tca
//
LOCUS       DASSWR010000611         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_220598,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000611 DASSWR010000000
VERSION     DASSWR010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220598"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFO47_10160"
     CDS             <1..485
                     /locus_tag="VFO47_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET8789099.1"
                     /translation="IGTEGTIGSGRYREAIEETHENVTLVAQACPRFVEYVERGETTG
                     PEILELARGYLAPLLAAEVDTLILGCTHYPLLTGVIGYLVGPDVFLVNSAEWTARSVF
                     AELTRADLHAPYGARPAHRFLASGDPDEFRRVGARFLGPEVGVVERRSAAVPVEAEAS
                     "
     gene            482..1249
                     /locus_tag="VFO47_10165"
     CDS             482..1249
                     /locus_tag="VFO47_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8789100.1"
                     /translation="MIRLTVLGSSGTYPAAGRACSGYLLEAREGDQATRVWVDTGPGT
                     LSNLLRVCDLPTLDAIWVSHLHVDHVGDLLAANYALRYGDFQPPPVPVPVFGPTDWVS
                     HVRPFLSTGESEPIDGTFEVHDLHDGEQVDLGPMRLEAVATVHSVETYAVRVTAGGHT
                     LAYSADSGSCDALGRLADQADLFVCEAAWPEWPEGAPPIHLTPQLAGEWAAKAKARRL
                     LLTHLRPGSDVGVATARAAEAYGGPVGVATELEVHHI"
     gene            1249..2052
                     /gene="rph"
                     /locus_tag="VFO47_10170"
     CDS             1249..2052
                     /gene="rph"
                     /locus_tag="VFO47_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET8789101.1"
                     /translation="MGRKDGRTPDQLRPVKITLGWQETAFASALVELGRTKVVCAVSY
                     EDRVPPFLRGSGKGWVTSEYAMLPGATGERSSREVSKGRPGGRTHEIQRLIGRSLRAV
                     TDTEAFGESTLWVDCDVLQADGGTRCASITGAWVALFAASHRLMQDKRIKSFPLRDHV
                     AAVSVGMIDGTPCLDLHYEEDVRAETDMNVVMTGLGGLVEVQGTAEREPFSRAELDAM
                     LDLAGTGIAALVKAQKQALGPMLEQAAIPPLRRLALGPSSLDLADGRAG"
     gene            2093..2716
                     /gene="rdgB"
                     /locus_tag="VFO47_10175"
     CDS             2093..2716
                     /gene="rdgB"
                     /locus_tag="VFO47_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET8789102.1"
                     /translation="MKPVLLLASANQGKLRELRTILEGLPMELVGLGDVGPGEPPEVD
                     ETGDTFLENALLKARAYAAWSGLAAVADDSGLEVDALGGAPGVRSARYAGPGAGDQAN
                     LDKLLAALAGVPPERRTARFRCAAVLVDPGAEGREWHAEAAWEGRVLDTPRGEGGFGY
                     DPVFLPDGWELTSAEVDQATKDAASHRGRAFRALRPAIEAWARSAAG"
     gene            2764..>2982
                     /locus_tag="VFO47_10180"
     CDS             2764..>2982
                     /locus_tag="VFO47_10180"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8789103.1"
                     /translation="MDGVRLVASDLDGTLLRPDETVSERTQRAVAAARDAGITVVLVS
                     GRPPRNIGWLAERVGVGGIAICANGAVIW"
BASE COUNT          373 a         1126 c         1116 g          367 t
ORIGIN      
        1 tgatcggcac cgaggggacg atcggctccg gccgctaccg cgaggccatc gaggagaccc
       61 acgagaacgt caccctggtc gcccaggcct gcccccggtt cgtcgagtac gtggagcggg
      121 gcgagaccac cgggccggag atcctggagc tggcccgcgg ctacctggcc ccgctgctgg
      181 ccgccgaggt cgacaccctg atcctcggct gcacccacta cccgctgctc accggggtca
      241 tcggctacct ggtgggcccg gacgtgttcc tggtcaactc ggccgagtgg accgcccgca
      301 gcgtcttcgc cgagctgacc cgggccgacc tccacgcccc ctacggggcc cgcccggccc
      361 accggttcct ggcctccggc gaccccgacg agttccgccg cgtcggcgcc cgcttcctcg
      421 gcccggaggt cggcgtcgtg gagcggcgca gcgccgccgt gccggtcgag gccgaggcct
      481 cgtgatccgg ctcaccgtgc tcggcagctc cgggacctac ccggccgccg gccgcgcctg
      541 ctccgggtac ctgctggagg cgcgggaggg cgaccaggcc acccgggtct gggtcgacac
      601 cggccccggc accctgtcca acctgctccg ggtgtgcgac ctgcccaccc tggacgccat
      661 ctgggtcagc cacctgcacg tcgaccacgt cggtgacctg ctggcggcca actacgccct
      721 gcgctacggc gacttccagc cgccgccggt gcccgtgccg gtgttcggcc ccaccgactg
      781 ggtcagccac gtgcggccgt tcctgtccac cggcgagagc gagcccatcg acggcacctt
      841 cgaggtccac gacctgcacg acggcgagca ggtcgacctg gggccgatgc ggctggaggc
      901 ggtggccacc gtccactcgg tcgagaccta cgcggtgcgg gtcacggccg gcggccacac
      961 cctcgcctac tcggccgact cggggtcgtg cgacgccctc gggcgcctcg ccgaccaggc
     1021 cgacctgttc gtctgcgagg ccgcctggcc ggagtggccg gagggcgcgc ccccgatcca
     1081 cctgaccccg cagctggccg gcgagtgggc ggccaaggcc aaggcgaggc gactgctcct
     1141 cacccacctg cgccccggca gcgacgtcgg ggtggccacg gcccgcgccg ccgaggccta
     1201 cggcggcccg gtgggcgtcg ccaccgaact ggaggtacac cacatctgat gggcaggaaa
     1261 gacgggcgga ccccggacca gctgcggccg gtcaagatca ccctcggctg gcaggagacg
     1321 gccttcgcct ccgcgctggt cgagctgggc cggaccaagg tggtgtgcgc ggtcagctac
     1381 gaggaccggg tgccgccgtt cctgcggggc agcggcaagg gctgggtgac ctccgagtac
     1441 gccatgctgc cgggggcgac cggggagcgg tcgtcccgcg aggtgtccaa gggccgcccc
     1501 ggcggccgca cccacgagat ccagcgcctg atcggccggt ccctgcgggc ggtcaccgac
     1561 accgaggcct tcggcgagtc gaccctgtgg gtcgactgcg acgtcctcca ggccgacggc
     1621 ggcacccgct gtgcctccat caccggcgcc tgggtggccc tgttcgcggc cagccaccgg
     1681 ctgatgcagg acaagcggat caagtcgttc ccgctccgcg accatgtggc cgcggtcagc
     1741 gtcgggatga tcgacgggac cccctgcctc gacctccact acgaggagga cgtgcgcgcc
     1801 gagaccgaca tgaacgtggt catgaccggg ctcggcggcc tggtcgaggt ccagggcacg
     1861 gccgagcggg agccgttcag ccgggccgag ctggacgcca tgctcgacct ggccggcacc
     1921 ggcatcgccg ccctggtcaa ggcccagaag caggccctgg ggccgatgct ggagcaggcg
     1981 gccatcccgc ccctgcgccg cctcgccctc ggcccgtcct ccctcgacct cgccgacggc
     2041 cgcgccgggt gagccggcgg aggggcccgg ggaaccccag gggggtgccc cggtgaaacc
     2101 ggttctcctg ctggccagcg ccaaccaggg caagctgcgc gagctgcgga cgatcctgga
     2161 ggggctgccg atggagctgg tcgggctcgg tgacgtcggc ccgggcgagc cacccgaggt
     2221 cgacgagacc ggcgacacgt tcctggagaa cgccctgctc aaggcgcggg cctatgccgc
     2281 ctggtcgggg ctggccgcgg tcgcggacga ctccggcctg gaggtggacg ccctcggcgg
     2341 cgcccccggg gtccgctcgg cccgctacgc cggcccgggc gccggcgacc aggccaacct
     2401 ggacaagctc ctggccgccc tggccggggt cccccccgag cggcgcacgg cccggttccg
     2461 ctgcgccgcc gtcctggtcg atcccggggc ggagggaagg gagtggcacg ccgaggcggc
     2521 ctgggagggg cgggttctgg acacccccag aggggaaggc gggttcggct acgacccggt
     2581 gttcctgccc gacggctggg agctgaccag cgccgaggtc gaccaggcga ccaaggacgc
     2641 cgcctcccac cgtggccggg cgttccgggc gctgcgcccg gccatcgagg cctgggcgcg
     2701 gtcggccgcg ggctagccgg ccggtcgcgt ccgggcgccg gaccgacccg ctaccgtgag
     2761 ggcatggacg gtgtccggct ggtggccagc gacctcgacg ggaccctgct gcggcccgac
     2821 gagacggtgt cggagcggac ccagcgggcc gtggccgcgg ccagggacgc cgggatcacg
     2881 gtggtgctgg tcagcgggcg gccgccccgg aacatcgggt ggctggccga gcgggtcggg
     2941 gtcgggggca tcgccatctg cgccaacggc gccgtgatct gg
//
LOCUS       DASSWR010000612         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_446913,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000612 DASSWR010000000
VERSION     DASSWR010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446913"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFO47_10185"
     CDS             <1..834
                     /locus_tag="VFO47_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8789104.1"
                     /translation="KGPELLNKFVGETERHIRLIFQRAREKASEGTPVIVFFDEMDSI
                     FRTRGSGVSSDVENTIVPQLLSEIDGVEQLKNVIVIGASNREDMIDPAILRPGRLDVK
                     IKIERPDASAARDIFSKYLLPSLPLAEDVVASAGGDREAALSRIIDEGVKRMYSDADE
                     NRFLEVTYANGDKEVLYFKDFNSGAMIENIVSRAKKMAIKRFLETGEKGIKAADLFAA
                     VTDEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLIEGGNSKDLGRTIEQVNAGQY
                     L"
     gene            875..2359
                     /gene="dop"
                     /locus_tag="VFO47_10190"
     CDS             875..2359
                     /gene="dop"
                     /locus_tag="VFO47_10190"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HET8789105.1"
                     /translation="MAITKVMGIETEYGITVPGAPDSNPVLASSLLVNAFSGRGRKVR
                     WDYEEESPLRDARGFEAAREPETPAEDDLGLANVILTNGARYYVDHAHPEYSSPECLD
                     PRTLVTYDKAGERILAESARRAAELAPGGRRIQVYKNNTDGKGTSYGTHENFLVDRAT
                     PFARIVRDLTPFLVSRQVFAGAGSLVRDHHDGSVAFQIAQRSQFFEVEVGLETTLKRP
                     IVNTRDEPHADPERYRRLHVIIGDANMNEVATFLKTGSLAIVLSMIEDGVIPGDEWQL
                     ANPVQALRAIAKDPTLRTVVERKDGRRLTACELQWHYLDAAKTYLKDRETSHWQIEVL
                     ERWEAVLALLEDDPLKASRTVDWATKYQLMQQMADRHGLEWEDAKLAVVDIQYHDVRM
                     DKGLYYRLAASGRVERLVAEQEITTAVSEPPADTRAYFRGKCLARYAPQIAAAGWDAI
                     IFDIGRETLQRVPMLEPTRGTAAHTAAVLDASPTAEDLIRNLQS"
     gene            2446..2658
                     /locus_tag="VFO47_10195"
     CDS             2446..2658
                     /locus_tag="VFO47_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HET8789106.1"
                     /translation="MARDSGGAEQKRRTPGKQEAEQEEAQASPDIQEKGEELKEELDA
                     LLDEIDDVLEENAEEFVKQYVQKGGE"
BASE COUNT          505 a          963 c          879 g          348 t
ORIGIN      
        1 aaaggccccg aactgctgaa caagttcgtc ggcgaaaccg agcgccacat tcggttgatc
       61 ttccagcgcg cccgggagaa ggcttccgag ggcactccgg tgatcgtgtt cttcgacgag
      121 atggactcga tcttccgcac ccgcgggtcg ggcgtgtcct ccgacgtcga gaacaccatc
      181 gtgccccagc tgctgtccga gatcgacggg gtcgagcagc tcaagaacgt gatcgtgatc
      241 ggcgcctcca accgcgagga catgatcgac ccggcgatcc tgcggcccgg ccgcctggac
      301 gtgaagatca agatcgagcg gcccgacgcc tccgcggcca gggacatctt ctccaagtac
      361 ctgctgccgt cgctgcccct ggccgaggac gtggtcgcct cggccggcgg cgaccgcgag
      421 gccgccctgt cccggatcat cgacgagggc gtcaagcgga tgtactccga cgccgacgag
      481 aaccggttcc tcgaggtcac ctatgccaac ggcgacaagg aggtcctgta cttcaaggac
      541 ttcaactccg gggcgatgat cgagaacatc gtcagccggg ccaagaagat ggccatcaag
      601 cggttcctgg agacggggga gaagggcatc aaggcggccg acctgttcgc cgccgtgacc
      661 gacgagttca aggagaacga ggacctgccc aacaccacca acccggacga ctgggcccgc
      721 atctcgggca agaagggcga gcgcatcgtg tacgtgcgga ccctgatcga gggcggcaac
      781 tccaaggacc tcgggcgcac catcgagcag gtcaacgccg ggcagtacct gtaggctgcg
      841 gcctggggga gtgctcggag aggggaacga acggatggcc atcaccaagg tcatgggcat
      901 cgagaccgag tacggcatca ccgtgcccgg cgcgcccgac tccaacccgg tgctggcctc
      961 cagcctcctg gtcaacgcct tctcgggccg cggccgcaag gtccgctggg actacgagga
     1021 ggagtcgccg ctgcgcgacg cccgcgggtt cgaggcggcc cgcgagcccg agaccccggc
     1081 cgaggacgac ctcgggctgg ccaacgtcat cctgaccaac ggggcccgct actacgtcga
     1141 ccacgcccac ccggagtact cctcgcccga gtgcctggac ccccgcaccc tggtcaccta
     1201 cgacaaggcg ggggagcgga tcctggccga gtcggcccgg cgggcggccg agctggcccc
     1261 gggcggccgc cgcatccagg tctacaagaa caacaccgac ggcaagggca cctcctacgg
     1321 cacccacgag aacttcctgg tcgaccgggc gaccccgttc gcccggatcg tccgcgacct
     1381 gaccccgttc ctggtcagcc ggcaggtctt cgccggcgcg ggcagcctgg tccgcgacca
     1441 ccacgacggc tcggtcgcct tccagatcgc ccagcggtcc cagttcttcg aggtcgaggt
     1501 cggcctggag acgaccctca agcgcccgat cgtcaacacc cgcgacgagc cccacgccga
     1561 ccccgagcgc tatcgccgcc tgcacgtgat catcggcgac gccaacatga acgaggtggc
     1621 caccttcctc aagacggggt cgctggccat cgtcctcagc atgatcgagg acggcgtcat
     1681 ccccggcgac gagtggcagc tggccaaccc ggtccaggcc ctgcgggcca tcgccaagga
     1741 cccgaccctg cgcaccgtcg tggaacgcaa ggacggccgc cgcctgaccg cctgcgagct
     1801 ccagtggcac tacctggacg cggccaagac ctacctcaag gaccgcgaga ccagccactg
     1861 gcagatcgag gtcctggagc gctgggaggc ggtcctggcc ctgctcgagg acgaccccct
     1921 gaaggcgtcc cggaccgtcg actgggcgac caagtaccag ctcatgcagc agatggccga
     1981 ccgccacggc ctggagtggg aggacgccaa gctggccgtg gtcgacatcc agtaccacga
     2041 cgtccgcatg gacaagggcc tctactaccg gctggccgcc tccgggcggg tcgagcgcct
     2101 ggtcgccgag caggagatca ccaccgccgt gtccgagccc ccggccgaca ccagggcgta
     2161 tttccgcggc aagtgcctcg cccgctacgc cccccagatc gccgccgccg gctgggacgc
     2221 gatcatcttc gacatcggcc gcgagaccct ccagcgggtc cccatgctcg agccgacccg
     2281 gggcaccgcc gcccacaccg cagccgtcct cgacgccagc ccgaccgccg aggacctgat
     2341 ccgcaacctc cagagctgat cccatcctcc acagggctca ggcaccagcc ccgccggtgg
     2401 cctggtcgtg gaacaatcgc ctcaacgccc gttcagggag gcacgatggc cagagacagc
     2461 ggcggcgccg agcagaagcg gcgcacgccc ggcaagcagg aagccgagca ggaagaggct
     2521 caggcgtccc ctgacatcca ggagaagggc gaggagctga aggaggagct cgacgccctc
     2581 ctggacgaga tcgacgacgt gctcgaggag aacgccgagg agttcgtgaa gcagtatgtc
     2641 cagaaaggcg gagagtagag ctttgtccgt ttaccatagg aactcggcgt gagga
//
LOCUS       DASSWR010000613         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_757069,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000613 DASSWR010000000
VERSION     DASSWR010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757069"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFO47_10200"
     CDS             complement(<1..903)
                     /locus_tag="VFO47_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET8789107.1"
                     /translation="MTEGPGGSGDFFGRIPLFREFARLMATASGPVNWELARQVAVAT
                     AAGAEDLGMMPQLPRPAAAPDPGEARSWDDRLRLAELWIDPVTSLPGPGVALAARPVN
                     RPDWAEAVLGGFAPLVEPGARRVAEALGSGAGMGPPGVGDMISRVGGLVAGLQVGTLV
                     GQLARSVLGQYELAMPGPDPAKVLLVPENVAAFERSADLPSDQLRLWLACHQVAGQRL
                     LAGVPWLGDQLRRVVDDVALQTEPDASQMMDRLQSLDMANPEALQELLAGGGEGLLGP
                     PSAALQAATGRLEVLLALVAGYTLV"
     gene            1021..2106
                     /locus_tag="VFO47_10205"
     CDS             1021..2106
                     /locus_tag="VFO47_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789108.1"
                     /translation="MGALHPAAGALALALEDDPGVERVLGLSRHEPPLLGPKFEFVQV
                     GPGDPTFAGTLAGADTVVLFPLVDAADRNEEGRRNRVLEGTRHALEAAAGAATVVLWS
                     SGVVYGAHPDNPVPITEDQPLRPNPEFPAAGVLADSERLALEASLGEGTAVVVLRGAG
                     VWTPAWGTFLSRSLMAPAMVGVQGQDPPLQCLAPADAVSALVLATSGRLRGVYNVAPD
                     DWIGARDAARAAGAGRRRLQVPQRIAQVTAERLQRAGVSAATPGELAYHLHPWVLDNA
                     RLRQAGWAPTRSSAEAFTEAGGQVPDGVLVGGVQVRRSDIYRGAVAGLALAAAAVAVA
                     RRQARASRSARPATRRPASRRPPSSPM"
     gene            2113..3123
                     /locus_tag="VFO47_10210"
     CDS             2113..3123
                     /locus_tag="VFO47_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8789109.1"
                     /translation="MARHLAIDLGTANTLVYAKGRGIALDQPTVIAINERSGDILAMG
                     EEAYAMIGRTPGHIVAVRPMRGGAISDFDTTARLIRMLLDRVGTGRLGKPRVLVCVPS
                     AITEVERRAVQEATLQAGARAAWLVEEPMAAAIGAGLPVHEPTGHLVVDVGGGTTEVA
                     CIALGGIVAAKAIRVGGFDMDAAIATHIRREYAVVVGERTAEQVKLSIGSAYPQPGEG
                     KAEVTGRELATGLPKTVPVSAAEVREALREPVGQIAQAVVSTLAEIPPELAGDVLDKG
                     IYLTGGASMLRGLHARLRHEAQIAVHQAEAPLECVVLGAGRIIEDIDDLAPLFTGDPA
                     RR"
     gene            3177..>3805
                     /locus_tag="VFO47_10215"
     CDS             3177..>3805
                     /locus_tag="VFO47_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET8789110.1"
                     /translation="MRQRVIAVVAVLGLIGLVFALNFYRLPVVALSPGPMEDVLARLK
                     VEGSRVYDSEGKLYLTSVGIDDNVRFYEALLDLANRDVELVPRSDLYPDEQDSDEIDE
                     ENTVLMDMSKETATVVALREVGYKIEPVGVEITQVVDGTPADGKLRARDRILEIDGRA
                     VASTDEVRKAITRHQVGERVAFRVERDEAEKKVSVEVGESEGQPRVGIAL"
BASE COUNT          495 a         1429 c         1438 g          443 t
ORIGIN      
        1 caccagggtg tagccggcca cgagggcgag cagcacctcc aggcgcccgg tggccgcctg
       61 gagggcggcg ctcggcgggc ccagcagacc ctccccgccg ccggcgagca gctcctggag
      121 ggcctcgggg ttggccatgt ccagcgactg gagccggtcc atcatctggg aggcgtcggg
      181 ctcggtctgg agggcgacgt cgtccaccac ccggcggagc tggtcgccga gccacggcac
      241 cccggcgagc aggcgctggc cagcgacctg atggcaggcc agccacagcc gcagctggtc
      301 gctgggcagg tcggccgacc gctcgaaggc ggccacgttc tcgggcacca gcagcacctt
      361 ggccgggtcg ggccctggca tggccagctc gtactggccg agcacggagc gggcgagctg
      421 gccgacgagc gtgcccacct ggaggccggc gaccaggccc ccgacccggc tgatcatgtc
      481 cccgaccccg ggcgggccca tgccggcccc gctgccgagg gcctcggcga cccggcgggc
      541 gcccggctcg accagggggg cgaagccgcc gaggacggcc tcggcccagt cggggcggtt
      601 caccggccgg gccgccaggg ccaccccggg gccgggcagg ctggtcaccg ggtcgatcca
      661 gagctcggcc aggcgcagcc ggtcgtccca ggagcgcgcc tcgcccgggt cgggggcggc
      721 cgccggccgc ggcagctgcg gcatcatgcc gaggtcctcg gccccggccg cggtggccac
      781 cgccacctgc cgggccagct cccagttgac cggcccggac gcggtcgcca tcagcctggc
      841 gaactcccgg aacaacggga tccgcccgaa gaaatcgccg gagccgcccg gcccttcggt
      901 catgggtctc atgctacccg cggcgtcacg acaagacggc tagaacccgc cccctccggg
      961 tagcctgggc gcttctggac ggcgacgaaa ggcaccacgg tgggtcggac ggtggcaatg
     1021 gtgggcgcgc tgcaccccgc ggcgggggcg ctggcgctgg ccctggagga cgacccgggg
     1081 gtcgagcggg tgctggggct gtcccggcac gagccgccgc tgctggggcc caagttcgag
     1141 ttcgtccagg tcggtcccgg cgacccgacc ttcgccggaa cgctggccgg ggcggacacg
     1201 gtggtgctgt tcccgctggt cgacgccgcc gaccgcaacg aggaggggcg ccggaaccgg
     1261 gtgctggagg ggaccaggca cgccctggag gccgccgccg gcgcggccac ggtggtgctg
     1321 tggtcgagcg gggtcgtcta cggcgcccac cccgacaacc cggtcccgat caccgaggac
     1381 cagccgctgc ggcccaaccc cgagttcccg gcggccggcg tgctcgccga cagcgaacgg
     1441 ctggcgctgg aggcgtcgct gggagagggc acggcggtgg tggtgctgcg cggcgccggg
     1501 gtgtggacac cggcctgggg gaccttcctg tcccggagcc tgatggcgcc ggccatggtc
     1561 ggggtccagg gccaggaccc gccgctgcag tgcctggccc ccgccgacgc cgtctcggcc
     1621 cttgtcctgg ccacctcggg ccggctgcgc ggcgtctaca acgtcgcccc cgacgactgg
     1681 atcggggcca gggacgccgc ccgggcggcc ggggccggcc ggcgccgcct ccaggtgccg
     1741 cagcggatcg cccaggtcac cgccgaacgg ctccagcggg ccggcgtctc ggccgcgacc
     1801 ccgggcgagc tcgcctacca cctccacccg tgggtgctcg acaacgcccg gctgcgccag
     1861 gccggctggg ccccgacccg ctccagcgcc gaggcgttca ccgaggcggg cgggcaggtg
     1921 cccgacgggg tcctggtcgg gggggtccag gtccgccgaa gcgacatcta ccggggcgcc
     1981 gtggccgggc tcgccctggc cgccgccgcc gtggctgtcg cccgccgcca ggcccgggcc
     2041 tcgcgctcgg cccgtccggc gacacgccgg ccggcctcca gacgcccccc ttccagcccc
     2101 atgtagccgg ccgtggcccg ccacctcgcc atcgacctgg gcacggccaa caccctggtc
     2161 tacgccaagg gcagggggat cgccctcgac cagccgaccg tgatcgcgat caacgagcgc
     2221 agcggcgaca tcctggccat gggcgaggag gcgtacgcca tgatcgggcg cacgcccggc
     2281 cacatcgtgg ccgtccggcc gatgcggggt ggagccatct ccgacttcga caccaccgcc
     2341 cggctcatcc ggatgctgct ggaccgggtc ggcaccggcc gcctgggcaa gccgcgggtg
     2401 ctggtctgcg tcccctcggc catcaccgag gtggagcggc gggccgtgca ggaggcgacc
     2461 ctccaggccg gggcccgggc cgcctggctg gtcgaggagc cgatggcggc ggccatcggg
     2521 gccgggctgc cggtgcacga gccgaccggc cacctggtgg tcgacgtcgg cggcggcacc
     2581 accgaggtgg cctgcatcgc cctcggcggc atcgtcgccg ccaaggcgat ccgggtgggc
     2641 ggcttcgaca tggacgcggc catcgccacc cacatccggc gcgagtacgc cgtcgtcgtc
     2701 ggcgagcgca ccgccgagca ggtcaagctg tcgatcgggt cggcctaccc gcagccgggg
     2761 gagggcaagg ccgaggtcac cggccgggag ctggccaccg ggctgcccaa gacggtcccg
     2821 gtctccgccg ccgaggtgcg cgaggcgctc cgggaaccgg tcggccagat cgcccaggcg
     2881 gtcgtctcga ccctcgccga gatccccccg gagctggccg gcgacgtgct cgacaagggc
     2941 atctacctga ccggcggggc cagcatgctg cgcggcctcc acgcccgcct gcgccacgag
     3001 gcgcagatcg ccgtccacca ggccgaggcg ccgctcgagt gcgtcgtgct cggcgccggc
     3061 cgcatcatcg aggacatcga cgacctggcg ccgctgttca ccggcgatcc cgcgcgccgc
     3121 tgaacggccc cggcggtgcg acaatcgagg atggttcccg actcccggag gtgccggtgc
     3181 ggcagcgcgt gatcgccgtc gtcgccgtgc tgggcctgat cggcctggtc ttcgccctca
     3241 acttctaccg gctcccggtg gtcgcgctca gccccggccc catggaggac gtgctggccc
     3301 gactcaaggt cgagggctcc cgggtctacg actccgaggg caagctctac ctcacctcgg
     3361 tcgggatcga cgacaacgtc cgcttctacg aggccctgct cgacctggcc aaccgggacg
     3421 tggagctggt cccgcggtcc gacctgtacc ccgacgagca ggactcggac gagatcgacg
     3481 aggagaacac cgtcctgatg gacatgtcca aggagacggc cacggtggtc gccctgcgcg
     3541 aggtcggcta caagatcgag ccggtcgggg tcgagatcac ccaggtcgtc gacggcaccc
     3601 cggccgacgg caagctgcgg gccagggacc gcatcctcga gatcgatggc cgcgccgtgg
     3661 ccagcaccga cgaggtccgg aaggcgatca cgcgccacca ggtcggcgag cgggtcgcct
     3721 tccgggtcga acgggacgag gccgagaaga aggtgtcggt ggaggtggga gagtcggagg
     3781 gccagccccg ggtcgggatc gcgct
//
LOCUS       DASSWR010000614         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_21045,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000614 DASSWR010000000
VERSION     DASSWR010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21045"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VFO47_10220"
     CDS             complement(<1..1021)
                     /locus_tag="VFO47_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8789111.1"
                     /translation="MTAELAAALRKAGVAEVDRSARRRAEYSTDASLYRVPPELVVFP
                     RDADEVVAVVATCLELGVPVTARGAGTSIAGNAVGPGVVMDLSRHLGRVLALDPEAAT
                     ARVEPGVVLDDLQAAARPHGLRFGPDPSTHSRCTLGGMLGTNACGSRALAYGRTADNV
                     LELDVVCGTGRRLRAGPGTVGEVPGLAAAVQAGLGPIRTQFGRFARQGSGYALEHLLP
                     ERGGDLARALVGSEGTLAVVLGATLRLVRAPAATTMVVLGYPDMATAADAVAGVLPAA
                     PVALEGIDARIVEVVRRRGRAVPELPEGGGWLLAELPGATAAEAEAAGRRLAASAGGD
                     ALAARV"
     gene            complement(1030..>2173)
                     /locus_tag="VFO47_10225"
     CDS             complement(1030..>2173)
                     /locus_tag="VFO47_10225"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HET8789112.1"
                     /translation="IAATGLPARVADQMKRFDIEAHVFDGVHVEPTDASLQEAIDHAA
                     EHGPWDAYVAVGGGSSIDTAKAVDLLATNPGELLDYVNRPVGEGRAPSRPLDPLVAVP
                     TTTGTGAESTTICVLDVLADKLKTGISHPRLRPTLAVVDPALTVTQPAAVTAASGMDV
                     LCHALESWTAKPYTAYERKRPEQRVPYCGANPISDLWSEQALRLLAGSFRDAVRDGDD
                     LEARGRMALAATMAGLGFGNAGVHIPHANAYPIAGRVKDFRPGGYPDGEPIVPHGMAV
                     SLTAPEAFRFTFEACPERHLRAAELLAPGLERPGDDAEYLPAALTRLMRDVGIPNGIG
                     GVGYDEADVPDLAEGAMKQQRLLDTAPRPVTEDDVAAILTRSLSNW"
BASE COUNT          241 a          819 c          857 g          256 t
ORIGIN      
        1 ggacccgggc ggcgagcgcg tccccgccgg ccgaggcggc cagccgccgc ccggccgcct
       61 cggcctcggc cgccgtggcc ccggggagct cggccaggag ccagccgcca ccctcgggga
      121 gctccggcac ggcccggccc cggcgccgta cgacctcgac gatgcgggcg tcgatgcctt
      181 cgagggccac cggcgccgcg ggcaggaccc cggccacggc gtcggccgcg gtggccatgt
      241 ccgggtagcc gagcacgacc atcgtggtcg ccgccggcgc ccggaccagg cggagggtgg
      301 ccccgaggac cacggccagg gtgccctcgc tgccgaccag ggcccgggcc aggtcgccgc
      361 cccgctcggg cagcaggtgc tccagcgcgt agccggagcc ctggcgggcg aagcgcccga
      421 actgggtccg gatcggcccc aggccggcct ggaccgccgc cgccagcccc ggtacctccc
      481 ccaccgtgcc cggcccggcc cgcaggcgcc ggcccgtgcc gcagaccacg tcgagctcca
      541 ggacgttgtc ggccgtgcgg ccgtaggcca gggcccgcga cccgcaggcg ttggtgccga
      601 gcatcccgcc cagggtgcag cggctgtggg tcgaggggtc ggggccgaag cgcagcccgt
      661 ggggccgggc cgcggcctgg aggtcgtcga ggaccacgcc cggctcgacc cgggccgtgg
      721 ccgcctccgg gtcgagggcc agcacccgcc ccaggtgccg ggacaggtcc atgaccaccc
      781 cgggcccgac ggcgttgccg gcgatcgagg tcccggcgcc cctggccgtc accggcacgc
      841 ccagctccag gcaggtcgcg accacggcca cgacctcgtc ggcgtcgcgc gggaagacga
      901 ccagctccgg cggcacccgg tacagcgagg cgtcggtcga gtactcggcc cgccgccgcg
      961 cggacctgtc gacctcggcc accccggcct tgcgcagggc ggcggccagc tcggcggtca
     1021 ggtcgcctcc taccagttcg acagcgagcg ggtcaggatc gcggcaacgt cgtcctcggt
     1081 caccggccgg ggggcggtgt cgagcagccg ctgctgcttc atggccccct cggccaggtc
     1141 gggcacgtcg gcctcgtcgt agccgacccc gccgatcccg ttggggatgc cgacgtcgcg
     1201 catcagccgg gtcagggcgg ccgggaggta ctcggcgtcg tcgccgggcc gctccaggcc
     1261 gggggcgagc agctcggccg cgcgcaggtg ccgctcgggg caggcctcga aggtgaaccg
     1321 gaacgcctcg ggcgcggtca gcgacacggc catgccgtgg ggcacgatcg gctcgccgtc
     1381 ggggtagccg ccgggccgga agtccttgac ccgcccggcg atcgggtagg cgttggcgtg
     1441 cgggatgtgg acgccggcgt tgccgaagcc gagcccggcc atggtcgcgg ccagggccat
     1501 ccgtccccgc gcctccaggt cgtcgccgtc gcgcacggcg tcgcggaacg agccggccag
     1561 cagccgcagc gcctgctccg accacaggtc ggagatcggg ttggccccgc agtagggcac
     1621 ccgctgctcc ggccgcttgc gctcgtaggc ggtgtagggc ttggccgtcc agctctccag
     1681 ggcgtggcag aggacgtcca tgccgctggc cgcggtcacc gcggccggct gggtcacggt
     1741 cagggccggg tcgaccacgg ccagggtcgg gcgcaggcgc gggtggctga tgccggtctt
     1801 gagcttgtcg gcgagcacgt cgagcacgca gatggtggtg ctctcggcgc ccgtgccggt
     1861 ggtcgtgggc acggccacca gcgggtcgag cggccggctg ggggcccggc cctcgccgac
     1921 gggccggttg acgtagtcga gcagctcgcc ggggttggtg gcgagcaggt cgaccgcctt
     1981 ggcggtgtcg atgctggagc cgccgccgac ggccacgtac gcgtcccatg gcccgtgctc
     2041 ggcggcgtgg tcgatcgcct cctggaggct ggcgtcggtc ggctcgacgt ggacgccgtc
     2101 gaacacgtgg gcctcgatgt cgaagcgctt catctggtcg gcgaccctgg ccggcaggcc
     2161 ggtggccgcg atg
//
LOCUS       DASSWR010000615         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_474484,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000615 DASSWR010000000
VERSION     DASSWR010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474484"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="hrcA"
                     /locus_tag="VFO47_10230"
     CDS             <1..911
                     /gene="hrcA"
                     /locus_tag="VFO47_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET8789113.1"
                     /translation="ATIRNELAVLEEQGYLAHPHTSAGRIPTDRGYRFYVDALSGVGE
                     LARAQEETIARFFEGAADLEETLQRTSLLLSSLTHYTAMVAPPALDQSRLRHIEVVGL
                     GRHVIMLVLIVDSGRVEKRLVETAEEISLEDLEALRRQLNERLANERLSRAELILDAM
                     AGEVPPERRALFQTLAAAIGQFVGDQTSERIWLGGQAHIAGPGAFDGIETVRQVYEAL
                     EQQVLVLRMLQAALGRDERVSVVIGSENTVEGMEACSLVTSAYLAGDASGSIGVLGPT
                     RMDYLRAMAAVQAVARYLGDAFDGNG"
     gene            915..2045
                     /gene="dnaJ"
                     /locus_tag="VFO47_10235"
     CDS             915..2045
                     /gene="dnaJ"
                     /locus_tag="VFO47_10235"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET8789114.1"
                     /translation="MSDYYQLLEVDRGAGAEEIKRAYRRLARQYHPDVNGNDPASAER
                     FKEINKAYSVLSDPDKRQRYDTYGEAGVEGFTGQGGDPFSTFGASFDDLLGQFFGGGD
                     PFGGGRSRTRSRARRGETIGMRLRLPFTEAVFGTSKDVRVRAATTCSRCSGSGAKPGT
                     EPTVCSRCNGSGQQRTVRQSLLGQLVTATTCPVCSGTGQEIRSPCPECQGQGLLMEDS
                     TLTIDIPPGLEDGSQIRYQGRGHAGQFGGPPGDLIVELAVDPHPVFDRRGDDLVCSVD
                     VPMTVAALGGSVPLETLDGNETVEIEPGTQSGAVKRFRKRGVPHPDGRGRGDLLVQIA
                     VVTPTHLGEQQRALIRQLAQLRGEEVDPQSGGLFARLKGSGR"
     gene            2042..>2497
                     /locus_tag="VFO47_10240"
     CDS             2042..>2497
                     /locus_tag="VFO47_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8789115.1"
                     /translation="MTAAAEAGGGLRYRSPTGRWVLLATVLGSGVAFLDATVVNIALP
                     AIGEDLDAGIASLQWVVNAYTLTLSGFLLLGGSLGDHYGRRRVFVLGVVWFALASLLC
                     GLAPTDEALIAARALQGVGGALLTPGSLAIIEASFRREDRGPAIGAWSGL"
BASE COUNT          328 a          962 c          880 g          327 t
ORIGIN      
        1 cggccaccat ccgcaacgag ctggccgtgc tcgaggagca gggctacctc gcccatcccc
       61 acaccagcgc cgggcggatc ccgaccgacc ggggctaccg gttctacgtg gacgccctgt
      121 ccggggtggg ggagctggcc cgggcccagg aggagaccat cgccaggttc ttcgagggcg
      181 ccgccgacct ggaggagacg ctgcagcgga cctcgctgct gctgtccagc ctcacccact
      241 acaccgccat ggtggccccg ccggccctcg accagtcgcg gctgcgccac atcgaggtgg
      301 tcgggctcgg gcgccacgtg atcatgctgg tgctgatcgt cgactccggg cgggtcgaga
      361 agcggctggt cgagacggcc gaggagatct cgctggagga cctggaggca ctgcgccgcc
      421 agctcaacga gcggctggcc aacgagcgcc tgtcgcgggc cgagctgatc cttgacgcca
      481 tggccggcga ggtcccgccc gagcgccggg ccctgttcca gaccctggcc gccgccatcg
      541 gccagttcgt cggcgaccag accagcgagc ggatctggct gggcggccag gcccacatcg
      601 ccgggccggg ggccttcgac gggatcgaga ccgtccgcca ggtctacgag gcgctggagc
      661 agcaggtcct ggtgctgcgc atgctccagg ccgccctggg ccgcgacgaa cgggtgtcgg
      721 tggtgatcgg ctcggagaac acggtcgagg ggatggaggc ctgctcgctg gtcacctccg
      781 cctacctggc cggcgacgcc agcggctcca tcggcgtcct cggcccgacc cgcatggact
      841 acctgcgggc catggccgcc gtgcaggcgg tggcccgcta cctcggtgac gccttcgacg
      901 gaaacgggta acgggtgagc gactactacc agctgctgga ggtcgaccgg ggtgccgggg
      961 ccgaggagat caagcgcgcc taccggcgcc tcgcccgcca gtaccacccg gacgtgaacg
     1021 gcaacgaccc ggcgtcggcc gagcgcttca aggagatcaa caaggcctac agcgtcctgt
     1081 ccgacccgga caagcgccag cgctacgaca cctacggcga ggccggggtc gagggcttca
     1141 ccggccaggg cggcgacccg ttctcgacct tcggggcctc cttcgacgac ctgctgggcc
     1201 agttcttcgg cggcggcgac ccgttcggcg ggggccgctc ccggacccgc tcgcgggccc
     1261 gccggggcga gaccatcggc atgcgcctgc gcctgccgtt caccgaggcg gtcttcggca
     1321 cctccaagga cgtccgcgtc cgggccgcca ccacctgctc acgctgcagc gggtcggggg
     1381 ccaagccggg caccgagccg accgtctgct cgcgctgcaa cggctccggc cagcagcgca
     1441 ccgtccgcca gtcgctcctc ggccagctgg tcacggccac cacctgcccg gtctgctccg
     1501 gcaccggcca ggagatccgc tcgccctgcc cggagtgcca gggccagggg ctgctgatgg
     1561 aggactccac cctcaccatc gacatccccc cgggcctgga ggacggcagc cagatccgct
     1621 accagggccg cggccacgcc ggccagttcg gcgggccccc cggcgacctg atcgtcgagc
     1681 tggccgtcga cccgcacccg gtcttcgacc gccgcggcga cgacctggtc tgcagcgtcg
     1741 acgtgcccat gacggtggcc gccctcggcg gcagcgtccc ccttgagacc ctggacggca
     1801 acgagacggt cgagatcgag cccggcaccc agtccggcgc ggtcaagcgc ttccgcaagc
     1861 ggggcgtgcc ccaccccgac ggccgcggcc gcggcgacct gctcgtccag atcgccgtcg
     1921 tcaccccgac ccacctcggc gagcagcagc gggccctgat ccgccagctc gcccagctcc
     1981 gcggcgagga ggtcgacccg cagagcggcg gcctgttcgc gcggctcaaa ggcagcggcc
     2041 gctgacggcg gcggccgagg ccggcggcgg gctgcgctac cgcagcccaa cgggccgctg
     2101 ggtgctgctg gccacggtgc tcgggtcggg cgtggccttc ctcgacgcca ccgtcgtcaa
     2161 catcgccctg ccggccatcg gcgaggacct ggacgccggc atcgccagcc tccagtgggt
     2221 ggtcaacgcc tacaccctca ccctgtcggg gttcctgctg ctcggcgggt ccctcggcga
     2281 tcactacggg cgccggcggg tgttcgtcct cggggtcgtc tggttcgcgc tggcgtcgct
     2341 gctctgcggc ctcgccccca ccgacgaggc cctgatcgcg gcccgcgccc tccagggcgt
     2401 cggcggcgcc ctgctcaccc cgggcagcct ggccatcatc gaggccagct tccggcgcga
     2461 ggaccgcggc ccggccatcg gcgcctggtc cggcctt
//
LOCUS       DASSWR010000616         7440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_616557,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000616 DASSWR010000000
VERSION     DASSWR010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616557"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VFO47_10245"
     CDS             <1..1388
                     /locus_tag="VFO47_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8789116.1"
                     /translation="AVRASRVAPLAALRAVAVDRSATSRRRAVAGAVVTGAGIALTIV
                     GATGEGLLPTGLGALATLTGVVVLGPVVARPAAAVLGAPQATWRGMSGVLAQRNAMRN
                     PRRTASTAAALLIGVAVVSLFAVVGASLKQSIDDAVDKQFAGDLVIIGEGAGGLSTDL
                     APAVAELPEVAAASPLGGGPVRIDGEGRLAPTFDPATIESVEHLDVLNGSLRELRPDQ
                     VAISADYAEQHGLALGDPVTVDYPDGVTERPTVGAIYAENNLSEGGGIMLHRDAYLPH
                     TSRPADVNLLIALADGVPLAEGEAAVQRVADRFGAPDVQTNQEFTESIAGEINLYLTV
                     VYLLLILAIVIAVMGIANTLSLSIHERTRELGLLRAVGQTRRQTRTMVRGEAVIVGLF
                     GTVGGLGLGLFLGWALVSSLASEGFGSFAVPKLSLAVVLALGALAGVLAAVRPAHRAA
                     RMNMLSAIATE"
     gene            1570..2223
                     /locus_tag="VFO47_10250"
     CDS             1570..2223
                     /locus_tag="VFO47_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET8789117.1"
                     /translation="MTDEPGQRGPSGEVRASDAEREAVVERLRVATVEGRLTLGELTE
                     RTEAAYTATTRGELVRITTDLPEVPGSTAVQARPAGGDREWVVAVMGDSKRQGRWRVE
                     RPLAALAVMGDVVLDLRGAEVPQGHVDITATAVMGDVKIYVPDGVDVQLSGIAVMGDK
                     KVAVREAPAGRTAPRVVVRATVVMGDVKVIGDSYAEPVRRTLRAWLTRDDRDRRAIE"
     gene            complement(2273..2449)
                     /locus_tag="VFO47_10255"
     CDS             complement(2273..2449)
                     /locus_tag="VFO47_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET8789118.1"
                     /translation="MDKEGFRDKADEALTQGEQEVDERTGGRFDEQTDTAAQEARERG
                     EGLLGDQQADEPKP"
     gene            complement(2533..3354)
                     /locus_tag="VFO47_10260"
     CDS             complement(2533..3354)
                     /locus_tag="VFO47_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET8789119.1"
                     /translation="MSRTVLITGASSGIGRATALELLRAGHTVYGGARRVESLDAVTA
                     AGGHALAMDVTSDEDRRRAVRTVLGQQGRIDVLVNNAGFGLYGAAEDVPLDQARYQLE
                     VNLFGPARLIQLVLPHLRRQRSGTIVNVSSMGGEITFPLGAWYHASKHALEAYSDALR
                     QEVRRFGVDVVLVQPGLIRTEFGDVTAGEVRELSGHGPYHDLAEGLATSTEALYREGG
                     RASDPSVVAATIRKAVESSSPKPRYPVGYMARPLLALNRFLPARLFDRVASSQLS"
     gene            complement(3364..4200)
                     /locus_tag="VFO47_10265"
     CDS             complement(3364..4200)
                     /locus_tag="VFO47_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET8789120.1"
                     /translation="MDKPSDPAEARRHREAVEAADEARHVVRAIEDFQDPSQEWRRLF
                     SELFGSFLLVLVAAGGGMVGQAFPGTIGRAAAVVAPALMVTAIILFMGKVSGAHLNPA
                     VSVAFSLRRDFPWRRVPGYVLVQLAGAVLAAWFLQAVVGVSSAYGATYPAAGWPAGDA
                     FLLEAVLTLGLVSVILGTASGAQNLGPIAAIGVGAYIALAGLWASPISGASMNPARSF
                     GPAAVAADLRSYWVYVAGPLVGAVAAVGIAFVLRGGGGGRSASAAAQGGLFTRARRPD
                     RS"
     gene            complement(4251..4634)
                     /locus_tag="VFO47_10270"
     CDS             complement(4251..4634)
                     /locus_tag="VFO47_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8789121.1"
                     /translation="MDLKLEVVPIPVSDVDAAKAFYTEQVGFNLDHDARPNEHMRVVQ
                     MTPPGSACSVVIGEGLPLGAPGSVKGVQLVVEDIDAVREALAGRGVGVSDVQQLGPEG
                     SPGSRFLFFDDPDGNTWAVQELKRS"
     gene            4719..5906
                     /gene="dinB"
                     /locus_tag="VFO47_10275"
     CDS             4719..5906
                     /gene="dinB"
                     /locus_tag="VFO47_10275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET8789122.1"
                     /translation="MKRATILHADLDAFYASVEQRDDPRLRGRPVIVGGGVVLAASYE
                     AKAHGVRTAMGGVQARRLCPRAVVVPPRMGVYAKASKAVFEVFRQTTPLVEGLSIDEA
                     FLDVGGLWRISGTPAEIAVRLRHEVRERVGLPITVGVARTKFLAKVASGVAKPDGLLV
                     VPPDGELAFLHPLPVERLWGVGPVTAGKLRALGVTTVGQVVRLGEPTLVSLVGQAAGR
                     HLHALAHNRDPRPVDTGRRRRSIGSQRALGRSPKSPDDLDAALMGLVERVTRRMRDAG
                     RTGRTVVLRLRFDDFSRATRSHTLPWPTSHTHTILAAARDLLAAAMPMIRRQGITLIG
                     IAVANLDDSGAIQLTLPFDRQSGTALDAALDEVRERFGSGAVTRAALLGRDPGLTVPL
                     LPD"
     gene            5969..6844
                     /locus_tag="VFO47_10280"
     CDS             5969..6844
                     /locus_tag="VFO47_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007461387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8789123.1"
                     /translation="MKLGLHYWNYSTPSDPAAIAGTLAETARVADQGGFSVLTVMDHY
                     FQMDQPGMCSADEPMLEAYTTLSYVAGLTERIALGVLVSGVMYRHPGLLAKIVTTLDV
                     LSGGRARLGIGASWYEREQAGLGVPVVPVAERFERLEETLQICLQMWSDDNGPYAGRH
                     YQLAETICVPPPISQPHPPIQVGGGGEKKTLLLVARYADAGNVFGSSPADVAGKLDVL
                     RSHCDAEGRDYDRIQKTVLAVRPALADVDGFLEAAGEYAALGVTEIEVMPDRHPVEFA
                     TQVAEQILPRLPDGP"
     gene            complement(6861..>7440)
                     /locus_tag="VFO47_10285"
     CDS             complement(6861..>7440)
                     /locus_tag="VFO47_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789124.1"
                     /translation="GEAGMNLDRRLRATDMNLDRRLRAAGRALREGSATQVDAAVGLR
                     EIVHAGRPQPPDPTAAPAGQPAPPAPAGELLRRTQRLALAVNLLLVLALGVTLVVVAG
                     GARRGLDPGASTQSTARTSPPASFPPATTQVTRTVVQVPEACLESAELADEIISALTR
                     NKRDNRLATALRDYSIANQACREAAAAPTAGG"
BASE COUNT          967 a         2692 c         2787 g          994 t
ORIGIN      
        1 cggcggtccg ggcctcccgc gtcgccccgc tggccgcgct gcgtgccgtc gccgtcgacc
       61 ggtccgcgac ctcgcggcga cgggccgtcg ccggtgccgt cgtcaccggg gcgggcatcg
      121 ccctgaccat cgtcggggcc accggcgagg gcctgctgcc caccggcctg ggcgcgctgg
      181 cgaccctgac cggtgtcgtg gtgctgggac ctgtcgtggc ccgccccgcc gccgccgtgc
      241 tcggcgcccc acaggcgacc tggcgtggca tgagcggggt gctggcccag cgcaacgcga
      301 tgcgcaaccc gcggcgcacc gccagcaccg ccgccgcgct gctgatcggc gtggccgtcg
      361 tgtcgctgtt cgccgtcgtc ggcgcgtcgc tcaagcagtc gatcgacgac gcggtcgaca
      421 agcagttcgc cggcgacctg gtgatcatcg gcgaaggcgc aggcggcctc agcaccgacc
      481 tggcccccgc ggtcgccgag ctgcccgagg tcgcggcggc ctcgcccctc ggcggcggac
      541 ctgtacggat cgacggtgag ggcaggctcg cccccacctt cgacccggcc accatcgagt
      601 cggtcgagca cctcgacgtg ctgaacggat cgctacgaga gctccgcccc gaccaggtgg
      661 ccatctcggc cgactacgcc gagcagcatg ggctggcgct cggcgacccg gtgaccgtcg
      721 actaccccga cggcgtgacc gagcggccga cggtcggggc gatctacgcc gagaacaacc
      781 tgagcgaggg tggcgggatc atgctgcacc gcgacgcgta cctgccccac acatcccgtc
      841 ccgccgacgt caacctgctg atcgccctgg ccgacggcgt gcccctcgca gagggcgaag
      901 cggccgtgca gcgggtggcc gaccggttcg gcgcccccga cgtgcagacc aaccaggagt
      961 tcaccgagtc gatcgccggc gagatcaacc tgtacctcac cgtcgtctac ctgctgttga
     1021 tcctggcgat cgtgatcgcc gtcatgggca tcgccaacac cctgtcgttg tcgatccacg
     1081 aacgcacccg cgagcttggc ctgctccggg ccgtgggcca gacccgccgc cagacccgga
     1141 cgatggtccg aggcgaggcc gtcatcgtcg gcctgttcgg caccgtcggc ggcctgggcc
     1201 tgggcctgtt cctcggctgg gcgttggtgt cgtcactggc cagcgagggc ttcggctcgt
     1261 tcgccgtccc gaagctgtcg ctggccgtgg tgctcgcgct cggtgccctg gccggggtgc
     1321 tggccgccgt ccgccccgcc caccgagccg cccgcatgaa catgttgtcc gccatcgcca
     1381 cggagtgacg gtcgcaactg agctgcggcg tcccttccat caacaggggg ccccagcgtg
     1441 cccgccgaca aggtggacga cccacccccg gctggtgcgg tgagcagccc gttacggatg
     1501 gcgggtcggc aggaggcgac ggtatgccgg gggtgtcatg gacgacctgt tgccacgtat
     1561 tgtcggggca tgaccgacga acctggccag cgcggcccgt ccggcgaggt ccgcgcctcc
     1621 gatgcggagc gcgaagcggt ggtggagcga ctgcgggtgg ccaccgtcga gggccggctc
     1681 acgctcggcg agctgaccga acggaccgag gccgcgtaca ccgccaccac ccggggcgag
     1741 ctggtccgca tcacaaccga cctgcccgag gtgcccggct ccacggcggt ccaggcgcgg
     1801 ccggctggcg gcgaccgcga gtgggtcgtc gcggtcatgg gtgacagcaa gcggcaggga
     1861 cgatggcggg tcgagcggcc gctcgcggcg ctcgccgtca tgggtgacgt ggtgctcgac
     1921 ctgcgcggcg ccgaggtgcc acaaggccac gtcgacatca ccgcgaccgc cgtcatgggc
     1981 gacgtcaaga tctacgtgcc cgacggcgtc gacgtacagc tgagcgggat cgcggtgatg
     2041 ggcgacaaga aggttgcggt gcgggaggcg ccggctgggc ggacggcccc cagggtggtg
     2101 gtcagggcca cggtcgtcat gggagatgtc aaggtcattg gcgactccta cgccgagccg
     2161 gtccgccgga cattgcgtgc ctggctgacg cgcgacgacc gcgaccgccg cgccatcgaa
     2221 tagcgccggg tcgcggtcgt cgttccgcgg ctgatgactc ccgactgccg aactagggct
     2281 tcggctcgtc tgcctgctgg tcgccgagca ggccctcgcc tcgttcacgg gcctcctgag
     2341 cggcggtgtc ggtctgctcg tcgaagcggc cgccggtgcg ctcgtcgacc tcttgctcgc
     2401 cctgggtgag ggcctcgtcg gccttgtcgc ggaacccttc cttgtccatg ccggaccacc
     2461 tccttggagg gaatggctgt accacgctca gcggattggg aaaccgcagc gctagtccga
     2521 caggcgcgcc ggctagctga gctggctgga ggcgacccgg tcgaacaggc gggccggcag
     2581 gaaccggttg agggcgagca ggggcctcgc catgtagccg accgggtagc ggggcttcgg
     2641 gctggaggac tccaccgctt tgcggatggt cgcggccacg accgacgggt ccgacgccct
     2701 gccgccctcc cggtagagcg cctcggtgct cgtcgccagc ccctcggcga ggtcgtggta
     2761 ggggccgtgg ccggacagct cgcggacctc gccggcggtg acgtcgccga actcggtcct
     2821 gatcaggccg ggctggacga ggacgacgtc gacgccgaac cggcgcacct cctgacggag
     2881 ggcgtcggag taggcctcga gggcgtgctt ggaggcgtgg taccaggcgc ccagggggaa
     2941 ggtgatctcc ccgcccatcg aggacacgtt gacgatcgtg cccgagcgct gccggcgcag
     3001 gtgggggagc accagctgga tcagccgggc cgggccgaac aggttgacct cgagctggta
     3061 gcgggcctgg tcgaggggga cgtcctcggc ggcgccgtag agcccgaacc cggcgttgtt
     3121 gaccaggacg tcgatcctgc cctgctggcc gaggacggtc cgcacggccc gccggcggtc
     3181 ctcgtcgctg gtgacgtcca tggccagggc gtggccgccg gcggcggtga cggcgtcgag
     3241 gctctcgacg cggcgggcgc cgccgtagac ggtgtggccg gcacgcagca gctccagcgc
     3301 ggtggcgcgc ccgatgcccg acgaggcccc ggtgatgagc acggtcctgg acatggtcgc
     3361 ccctcagctt cggtcgggcc gtctggcccg ggtgaacagg ccgccctggg cggcggccga
     3421 ggcggagcgg ccgccgccgc ctccgcggag gacgaaggcg atcccgacgg ccgcgacggc
     3481 gccgaccagg ggtccggcga cgtacaccca gtaggagcgg aggtcggcgg cgacggcggc
     3541 gggcccgaag ctccgggccg ggttcatcga ggcgccggag atcgggctgg cccagaggcc
     3601 ggccagggcg atgtaggcgc cgacgccgat ggccgcgatg gggccgaggt tctgggcgcc
     3661 cgaggcggtg ccgaggatga cgctgaccag gccgagggtg agcacggcct ccagcaggaa
     3721 cgcgtcgccg gccggccagc ccgcggccgg gtaggtcgcg ccgtaggccg acgacacgcc
     3781 gacgacggcc tgcaggaacc aggcggccag caccgcgccg gccagctgga cgagcacgta
     3841 gccggggacg cgccgccacg ggaagtcgcg gcgcagcgag aacgcgacgc tgacggcggg
     3901 gttgaggtgg gcgccggaca ccttgcccat gaacaggatg atcgccgtca ccatcagcgc
     3961 cggcgcgacc accgccgccg cccggccgat cgtgcccggg aacgcctggc cgaccatgcc
     4021 gccgccggcg gcgaccagca ccagcaggaa gctgccgaac agctcggaga acagccggcg
     4081 ccactcctgc gacgggtcct ggaagtcctc gatcgcccgc acgacgtgcc tggcctcgtc
     4141 ggccgcctcc acggcctcgc ggtgacgcct cgcctcagcg ggatcggatg gcttgtccat
     4201 gcggccgcca gcgtatccgg gtcgaccggc cgcccgcagc cggggcgagt ctaggagcgc
     4261 ttcagctcct ggacggccca ggtgttgccg tcggggtcgt cgaagaacag gaagcgcgag
     4321 cccgggctgc cctcgggacc cagctgctgg acgtcgctga cgccgacgcc gcggccggcc
     4381 agggcctcgc ggacggcgtc gatgtcctcg accacgagct ggacgccctt gaccgagccg
     4441 ggggcgccga gggggaggcc ctcgccgatc accaccgagc aggccgagcc ggggggcgtc
     4501 atctggacga cccgcatgtg ctcgttgggg cgggcgtcgt ggtcgagatt gaagccgacc
     4561 tgctcggtgt agaaggcctt ggcggcgtcg acgtcggaga cggggatggg gacgacctcg
     4621 agcttcagat ccatctgcgt ccttccggtt cggtggtgag cccgcattcc atcacggcgg
     4681 ctcgacagcc gctgcccggc ggtgggaaca tacgttcgat gaagcgggcg accatcctgc
     4741 acgccgacct ggacgcgttc tacgcctcgg tcgagcagcg ggacgacccc cggctgcggg
     4801 gccggccggt gatcgtcggc ggcggggtgg tgctggcggc cagctacgag gccaaggcgc
     4861 acggggtgcg gacggccatg ggcggggtgc aggcgcggcg gctgtgcccg cgggcggtgg
     4921 tggtgccgcc ccggatgggg gtgtacgcca aggccagcaa ggcggtgttc gaggtcttcc
     4981 ggcagacgac gccgctggtc gaggggctct ccatcgacga ggcgttcctg gacgtcggcg
     5041 ggctctggcg catctcgggc acgcccgccg agatcgccgt gcggctgcgg cacgaggtcc
     5101 gcgagcgggt ggggctgccg atcaccgtcg gcgtggccag gaccaagttc ctggccaagg
     5161 tggccagcgg cgtggccaag cccgacggcc tgctggtggt gccgcccgac ggcgagctgg
     5221 ccttcctcca tccactgccg gtcgagcggc tctggggggt cgggccggtc accgccggca
     5281 agctccgcgc cctcggcgtc accaccgtcg gccaggtggt gcggctgggg gagccgacgc
     5341 tggtgtcgct ggtcggccag gcggccggcc ggcacctgca cgccctggcc cacaaccgcg
     5401 acccacggcc ggtcgacacc ggccggcggc ggcgctcgat cgggtcccag cgggcccttg
     5461 gccgctcccc caagtcgccc gacgacctcg acgccgccct gatggggctg gtcgagcggg
     5521 tcaccaggcg gatgcgggac gccgggcgga ccggccggac ggtggtgctg cggctgcgct
     5581 tcgacgactt ctcgcgggcg acccgctccc acaccctgcc ctggccgacc agccacaccc
     5641 acaccatcct ggccgcggcc cgcgacctgc tggccgcggc catgccgatg atccgccggc
     5701 agggcatcac cctcatcggc atcgccgtgg ccaacctcga cgacagcggc gccatccagc
     5761 tcaccctgcc gttcgaccgc cagagcggca ccgccctcga cgccgccctc gacgaggtcc
     5821 gcgagcgctt cgggtcgggc gccgtcaccc gcgccgccct gctcggccgc gacccgggcc
     5881 tgaccgttcc gctgctgccc gactgaccgg cggcggcggc cccgggtcgc gatagggtgg
     5941 gcgtccagcg cagacgagag gagccgccgt gaagcttggc ctccactact ggaactactc
     6001 gaccccgtcc gacccggcgg cgatcgccgg gaccctggcc gagaccgcgc gggtcgcgga
     6061 ccagggcggg ttctcggtgc tcaccgtcat ggaccactac ttccagatgg accagccggg
     6121 catgtgctcg gccgacgagc cgatgcttga ggcctacacc accctcagtt atgtcgccgg
     6181 cctgaccgag cgcatcgccc ttggagtgct ggtctccggc gtgatgtacc gccacccggg
     6241 gctcctggcc aagatcgtca ccaccctgga cgtgctgtcc gggggccggg cgcgcctcgg
     6301 catcggcgcc tcctggtacg agcgcgagca ggccggcctc ggggtgccgg tcgtgccggt
     6361 ggcggagcgg ttcgagcgcc tggaggagac gctccagatc tgcctgcaga tgtggagcga
     6421 cgacaacggc ccctatgccg gccgccacta ccagctggcc gagacgatct gcgtcccccc
     6481 gccgatcagc cagccccacc cgccgatcca ggtcggcggg ggcggggaga agaagaccct
     6541 gctgctggtg gcccgctacg ccgacgccgg caacgtgttc gggagcagcc cggccgacgt
     6601 ggccggcaag ctcgacgtgc tccgctccca ctgcgacgcc gagggccgcg actacgaccg
     6661 catccagaag accgtcctgg ccgtgcgacc ggccctggcc gacgtcgacg gcttcctgga
     6721 ggcggccggc gagtacgccg ctctcggcgt gaccgagatc gaggtcatgc ccgaccgcca
     6781 cccggtcgag ttcgccaccc aggtcgccga gcagatcctg ccccggctcc ccgacgggcc
     6841 ttgaccgttc cgggtccggc ctagcctccg gccgtcggcg ccgccgccgc ctcacggcag
     6901 gcctggttcg cgatcgagta gtcgcgtaag gccgtggcca gccggttgtc gcgcttgttc
     6961 ctggtcagcg ccgagatgat ctcgtcggcc agctcggcgc tctcgaggca tgcctccggg
     7021 acctggacca cggtccgggt gacctgggtg gtggcgggcg ggaacgaggc gggcgggctc
     7081 gtcctggcgg tgctctgggt ggacgcgccg gggtcgaggc cgcgtcgtgc tccgcccgcc
     7141 accacgacca gggtgacgcc gagggccagc accagcagca ggttgacggc gagggccagc
     7201 cgctgggtcc tgcggagcag ctcgccggcc ggagcgggag gcgccggctg accggccggc
     7261 gccgcggtcg ggtccggcgg ctgcgggcgg ccggcatgga cgatctcccg caggccgacg
     7321 gcggcgtcga cctgggtggc gctcccctcc ctgagcgccc tgccggccgc ccgtagccgc
     7381 cggtcgaggt tcatgtccgt cgcccgtagc cgccggtcga ggttcatgcc ggcctccccc
//
LOCUS       DASSWR010000617         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_165051,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000617 DASSWR010000000
VERSION     DASSWR010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165051"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1175)
                     /locus_tag="VFO47_10290"
     CDS             complement(195..1175)
                     /locus_tag="VFO47_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8789125.1"
                     /translation="MTWLVWRQHRQQVLAAGIAMLAVALFLVPTGRQMHAAFDDLGLR
                     SCLPGASPSEFLTSDDGCASLSEQFSGRFQGLATTSLLLLFLPVVAGMFWGAPLVARE
                     VEHGTHRLVWTQGVTRLRWATVKLALVGGGVVLVAVAYALLVGWWVAPLNDATGYRFT
                     WLFFDQQGPVLVGYALFAFALGVLAGTLTRKVLPAMAITLGGFLATRVAVALLLRPRF
                     LPYQRRAYPTVSGTAAEPNQLAGDWVISRAVYSAQGVKLTPGSGSQALCDQATAASCV
                     AKYGAGSYNQQLFQPADRFWLFQGIETALFVLLAVALLLLAVRLVRRRVA"
     gene            complement(1172..2089)
                     /locus_tag="VFO47_10295"
     CDS             complement(1172..2089)
                     /locus_tag="VFO47_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8789126.1"
                     /translation="MTGSSALETTGLGKRYRRTWALRDCTLALPQGRIAALVGPNGAG
                     KTTLLHLAVGLLAPTAGAIRVLGGTPGRDPALLARVGFVAQDAPLYGDFNVADHLTMG
                     AKLNPRFDQALARDRLGRLGVPLDRRVGTLSGGQRAQVALAMALAKRPELLLLDEPVA
                     SLDPLARREFLQTLMGSVAEQGLTVLLSSHLLADLERVCDYLIVLQAARVQVLGPVDD
                     LLERHKLLVGPRRDPDRIAGVAAVVRATHTDRQSTLLVRTDGPVRDPALAVADVTLED
                     LVLAYLADPSAGTLPGPAAPAPPRLEART"
     gene            complement(2086..2478)
                     /locus_tag="VFO47_10300"
     CDS             complement(2086..2478)
                     /locus_tag="VFO47_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8789127.1"
                     /translation="MIEFHLDGRSRVSTYMQLVQQVRQALRVGLLEPGDQLPRVREVA
                     ESLAINPNTVLKAYRELEHDGLVEGRPGQGTFVLRSLAGPALASQVELRRELLGWLGR
                     ARAAGLDTEDVVALFETTLRASSEEGVA"
     gene            complement(2582..3499)
                     /locus_tag="VFO47_10305"
     CDS             complement(2582..3499)
                     /locus_tag="VFO47_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8789128.1"
                     /translation="MRKLVLFGMVGVLAQLIDGALGMAYGLTSTTLLVAVGTAPATAS
                     ATVHLAELGTTAVSGVSHWRFGNVDWRTVRWMTFPGFLGAFLGAVVLSSISGDTAKPW
                     VAGILSVLGVYVLFRFLGKVRGPRPRAGRPLPSALLAPLGLGAGFLDAVGGGGWGPVG
                     TPTLLASGRMEPRKVIGSIDTGEFLVALGASLGFLVSLSWSQVPLTLVMLLLAGGLVA
                     APLAAWLVKHLAPRKLGVIVGATILLTNARTLASTFGVEGAWQAAMYLAIVAVVGALA
                     AWRLTQSRQRRRAKALAAAAQPLPEPVGA"
     gene            3624..>3905
                     /locus_tag="VFO47_10310"
     CDS             3624..>3905
                     /locus_tag="VFO47_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018215876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET8789129.1"
                     /translation="MAQQGLSGAQVAERVARGQVNDVPSAPTRTIGQIVRANVLTRFN
                     LLLGSLLAVILVVGPLQDALFGLVIVANTAVGIVQEVRAKRTLDRLEVVN"
BASE COUNT          585 a         1353 c         1517 g          450 t
ORIGIN      
        1 cgttcgacgg ccagcgcgtt cgcgaggctg attcgggcga tgatccccgg ctcacgcatg
       61 cgggtcgctc ctcacctgca gggccgaact gctgttcggc ctctgaggag actctaccat
      121 ggcccaacgt tggaggtccc aggaggctac tgccccgaca acggcaccgg cgtgagctgc
      181 ccggtcggcc tcgtctaggc gacccggcga cggaccaggc ggacggcgag cagcagcagg
      241 gccacggcca ggaggacgaa cagggcggtc tcgatgccct ggaacagcca gaagcggtcg
      301 gcgggctgga acagctgctg gttgtaggag ccggcgccgt acttggccac gcacgaggcc
      361 gccgtcgcct ggtcgcagag cgcctgggac ccgctgccgg gggtgagctt gaccccctgg
      421 gcgctgtaga cggcccggct gatgacccag tcgccggcca gctggttggg ctcggccgcg
      481 gtgccgctga ccgtcgggta ggcgcgccgc tggtagggca ggaagcgggg gcggagcagc
      541 agggccacgg ccaccctggt ggccaggaac ccgccgaggg tgatggccat cgccggcagg
      601 accttgcggg tcagggtgcc ggccagcacg cccagggcga aggcgaacag ggcgtagccg
      661 accagcaccg gcccctgctg gtcgaagaac agccaggtga agcggtagcc ggtggcgtcg
      721 ttgagcggcg ccacccacca tccgacgagc agggcgtagg cgaccgcgac cagcacgacc
      781 ccgccgccga cgagggcgag cttgaccgtg gcccagcgca gccgggtcac gccctgggtc
      841 cagaccagcc ggtgggtgcc gtgctccacc tcgcgggcga ccagcggcgc cccccagaac
      901 atcccggcca cgacgggcag gaacagcagc agcaggctgg tcgtggccag cccctggaag
      961 cggccgctga actgctcgct gagcgaggcg cagccgtcgt cggaggtcag gaactcggat
     1021 gggctggcgc ctggcaggca gctccgcagg ccgaggtcgt cgaaggcggc gtgcatctgc
     1081 cggccggtcg ggaccaggaa cagcgccacc gccagcatcg cgatgccggc agccagaacc
     1141 tgctggcggt gctggcgcca gacgagccag gtcatgtccg cgcctccagc cggggagggg
     1201 ccggcgcggc cgggccgggc agagtgcccg ccgacgggtc ggccaggtag gccaggacca
     1261 ggtcctcgag ggtgacgtcg gcgacggcca gggccgggtc gcgcaccggg ccgtcggtgc
     1321 ggaccagcag ggtgctctgc cggtcggtat gggtggcccg gacgaccgcg gccaccccgg
     1381 cgatccggtc cgggtcgcgg cgggggccga ccagcagctt gtgccgctcg agcaggtcgt
     1441 cgaccgggcc gagcacctgc acccgcgccg cctggagcac gatcaggtag tcgcagaccc
     1501 gctccaggtc ggccagcagg tgcgacgaca gcagcacggt caggccctgc tcggccacgc
     1561 tgcccatcag ggtctggagg aactcgcgcc gggccagcgg gtcgaggctg gccaccggct
     1621 cgtccagcag cagcagctcg ggccgcttgg ccagggccat ggccagcgcc acctgggccc
     1681 gctggccgcc cgacagcgtg ccgacgcggc ggtcgagggg gaccccgagc cggcccagcc
     1741 ggtcacgggc cagggcctgg tcgaagcggg ggttgagctt ggcgcccatg gtcaggtggt
     1801 cggccacgtt gaagtcgccg tacagggggg cgtcctgggc gacgaagccg acccgggcca
     1861 gcagggccgg gtcccggccg ggcgtcccgc ccagtacccg gatggcgccc gcggtcgggg
     1921 ccagcagccc gacggccagg tgcagcaggg tggtcttgcc ggcgccgttg ggaccgacca
     1981 gggcggcgat ccggccctgg ggcagggcca gggtgcagtc ccgcagcgcc caggtgcgcc
     2041 ggtagcgctt gcccagcccg gtggtctcca aggcggagga gccggtcatg ccacgccctc
     2101 ctcggaggag gcccgcaggg tggtctcgaa cagggccacc acgtcctcgg tgtcgaggcc
     2161 ggcggcgcgg gcccggccca gccagcccag cagctcccgg cggagctcga cctggctcgc
     2221 cagggcgggg ccggccagcg agcggagcac gaaggtgccc tgcccgggcc ggccctcgac
     2281 caggccgtcg tgctccagct cgcggtaggc cttgagcacc gtgttggggt tgatggccag
     2341 ggactcggcc acctcccgga cccgggggag ctggtcgccc ggctcgagca gcccgacccg
     2401 cagggcctgg cggacctgct ggaccagctg catgtaggtc gagacccggg agcggccgtc
     2461 aaggtggaac tcgatcatcg ggcaccccta tctcattgtc gtaggacaat agatagagtg
     2521 ggccgcggcc ggccgcctgt caaccgccgg tcaggtcagg tgccggtcag aacgaccttc
     2581 ctcaggcgcc gacgggctcg ggcagcggct gggcggcggc ggccagggcc ttggcccggc
     2641 gccgctggcg ggactgggtc aggcgccagg cggccagggc gccgaccacg gccacgatgg
     2701 ccaggtacat ggcggcctgc caggccccct cgacgccgaa ggtcgaggcc agagtgcggg
     2761 cgttggtgag caggatggtg gcgccgacga tcaccccgag cttgcggggg gccaggtgct
     2821 tgaccagcca ggcggccagg ggggcggcga ccaggccgcc ggccagcagc agcatgacca
     2881 gggtcagcgg cacctggctc cagctgagcg agacgaggaa gccgaggctg gccccgaggg
     2941 cgaccaggaa ctcgccggtg tcgatcgagc cgatgacctt gcggggctcc atgcggccgg
     3001 aggcgagcag ggtgggcgtg cccaccgggc cccagccgcc gccgccgacg gcatccagga
     3061 agccggcgcc gaggccgagg ggggccagca gggccgaggg cagcggccgg ccggcgcggg
     3121 gccgggggcc gcggaccttg ccgaggaagc ggaacagcac gtagacgccg agcacgctga
     3181 ggatgcccgc cacccagggc ttggccgtgt cgccgctgat cgaggacagc accaccgccc
     3241 caaggaaggc gccgaggaag ccggggaagg tcatccagcg caccgtgcgc cagtcgacgt
     3301 tgccgaagcg ccagtgggag acgcccgaca ccgcggtggt gcccagctcg gccaggtgca
     3361 cggtggccga ggccgtcgcc ggggcggtgc cgaccgcgac cagcagggtg gtcgaggtca
     3421 ggccgtaggc catgcccagg gcgccgtcga tcagctgggc cagcaccccg accatcccga
     3481 acagcaccag cttgcgcatc gtccctccgg cgttcccgac caagttgatg ttgtctacca
     3541 gcttgctgga gatggtaagc atggcatgag gtagcaccat catgcgttgg cagtagtccg
     3601 ccctaccgca ataggagtgc gttgtggccc aacaggggct gagcggggcc caggtggccg
     3661 agcgggtggc ccgggggcag gtcaacgacg tgcccagcgc cccgacccgg accatcggcc
     3721 agatcgtccg ggccaacgtg ctgacccgct tcaacctgct gctggggtcg ctgctggccg
     3781 tcatcctggt cgtcgggccg ctccaggacg ccctgttcgg cctcgtgatc gtggccaaca
     3841 cggcggtcgg gatcgttcag gaggtgcggg ccaagcggac cctcgaccgc ctcgaggtgg
     3901 tcaac
//
LOCUS       DASSWR010000618         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_105957,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000618 DASSWR010000000
VERSION     DASSWR010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105957"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFO47_10315"
     CDS             <1..265
                     /locus_tag="VFO47_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789130.1"
                     /translation="QIPLWSDSRDAFIQATREFAPPGDYRFVATSANRQPAVAIYHRP
                     PGEPVFRLTALEVLRIEGDRVAEIVDFDVPDLYPAFGLEPTLP"
     gene            371..832
                     /locus_tag="VFO47_10320"
     CDS             371..832
                     /locus_tag="VFO47_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789131.1"
                     /translation="MTDASTSYDSPETPTPAPDIKALGERLLGAWRVSGGAEGVVRYE
                     WMEGGFFLVQHVELEQYGQQVKGMEIIGHLRPFGEPPSPDIHSRFFDSTGNTLDYVYE
                     LEGDTLTIWGGEKGSPAYYRGTFSPDGTSHSGDWVYPGGGGYASTSTRIQP"
     gene            complement(876..1310)
                     /locus_tag="VFO47_10325"
     CDS             complement(876..1310)
                     /locus_tag="VFO47_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789132.1"
                     /translation="MAEMARVLAPGGRLAAMVWRSIDHSPGFAALADALDRHIGPAAG
                     ATMRAPFTLNDEATLHDLLAGGGFEDVEVHPQAGTVRFGSVQEFLIAQGTGSPLAAPI
                     AAADPAARAALLADAETTLAPWQGPDGFAFPIGALLLSGRVP"
     gene            complement(1701..2081)
                     /locus_tag="VFO47_10330"
     CDS             complement(1701..2081)
                     /locus_tag="VFO47_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8789133.1"
                     /translation="MTMKLELVPIPVTDVDRAKAFYTDQVGFHADLDERFGDEFRVVQ
                     LTPPGSACSICIGVGIVQTPPGSVQALHLVVPDIHATRAELLERGVEISEVEDMTAPG
                     KPTVSYAYFKDPDGNGWALQQLPC"
     gene            2167..2760
                     /locus_tag="VFO47_10335"
     CDS             2167..2760
                     /locus_tag="VFO47_10335"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET8789134.1"
                     /translation="MSEIGTGTGGGQPLDGLREQVREAARAAAAHDLAPKPPRASEVT
                     LSQHMDLSNANFMGNVHGGEIMKLIDTAAGIAASRHAGGPVVTASLDQMSFLHPVHVG
                     DVVLVRASVNDVGRTSIEVGVRVEAEEILSGRRTHTSSAYLVFVALDERGQPRPVPGL
                     VPENDVERHRQAEAKIRREHRLARAEAIRRRRDQLDT"
     gene            2757..>3915
                     /locus_tag="VFO47_10340"
     CDS             2757..>3915
                     /locus_tag="VFO47_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789135.1"
                     /translation="MSGATRPGDDGAVTPAQVLAGLLLAIIVLWLLVPFAGVDPPRGL
                     TASNAPWTDEGFNLANARERVLTGQFATGDIDRSLTNGAYSGLAAAMFAVTGPSLAAG
                     RALSMVAVAAAVLLLALGLARPLGRPAALLAAAALGGANLMLEHGRLALVEATVVALL
                     AGAFVLATRAAWRPSPAAGIGLGLLVAAAVSVKAIALLPGMVMLAVVLVAAAVRRDRG
                     ALGMGLAGLAALVAAGGAWLAAVALPNWDRFRTSLRIWPSVEYLDGPAAIAGRLGRYL
                     ADSDQALGRSLPLLLAAALGVAALARRWPRLAPEARDALVMALVWGTGLWLAVAVADY
                     LPGNYGAPNRYVVPALPGLAVLAGFGLADLAALAGRALGRERAGWAAGAVGV"
BASE COUNT          489 a         1396 c         1528 g          502 t
ORIGIN      
        1 gcagatcccc ctgtggtccg actcgcggga tgccttcatc caggccacca gggaattcgc
       61 acctcccggt gactaccggt tcgtcgccac cagcgccaac cggcaaccgg cggtggcgat
      121 ctaccaccgg ccgccggggg agccggtgtt ccggctcacc gcccttgagg tgctgcgcat
      181 cgagggcgac agggtggccg agatcgtcga cttcgacgtc cccgacctgt acccggcgtt
      241 tggcctggag ccgaccctcc cctgaccgac gcgccggtcg cgccgttggg ggagcgattc
      301 ctttcggccc gcttcctcgt agcaactggt gaaggccgcc gacggcggcc ccgcacagga
      361 ggaaccggcc atgaccgatg cgtcgaccag ctacgacagc cccgagaccc cgaccccggc
      421 gccggacatc aaggccctcg gcgagcggct cctcggcgcc tggcgggtga gcggcggcgc
      481 cgagggcgtc gtccgctacg agtggatgga aggagggttc ttcctggtcc agcacgtcga
      541 gctcgagcag tacgggcagc aggtcaaggg catggagatc atcgggcacc tgcggccctt
      601 cggggagccg ccgagcccgg acatccactc ccgcttcttc gacagcacag gcaacaccct
      661 cgactacgtc tacgagctag agggcgacac cctcacgatc tggggcggcg agaaaggctc
      721 ccccgcctac taccgcggca ccttcagccc cgacggcacc agccactcgg gcgactgggt
      781 gtacccggga ggcggcggct acgcgtcgac cagcaccagg atccagccct gagctccggc
      841 acccacaggg aggcgtcggg cataggccgt gtggctcagg gaacacgccc gctgagcagg
      901 agggccccga tcgggaaggc gaacccgtcg gggccctgcc acggggccag ggtggtctcg
      961 gcgtcggcga gcagagccgc tcgggcggcg gggtcggcgg cggcgatagg ggcggccagg
     1021 ggcgacccgg tgccctgggc gatcaggaat tcctgaaccg agccgaaccg gaccgtgccc
     1081 gcctgggggt gcacctccac gtcctcgaac ccgcctccgg cgagcaggtc atgcagcgtc
     1141 gcctcgtcgt tgagggtgaa cggcgcccgc atggtcgcgc cggcggccgg gccgatgtgg
     1201 cggtcgaggg cgtcggccag ggcggcgaac cccgggctgt ggtcgatcga gcgccacacc
     1261 atggccgcca ggcggccacc cggggccagc accctggcca tctcggccac ggcggcgggg
     1321 cggtcgggga agtactggag gccgagctgg caggcgacga cctcgaagct gccgtcggcg
     1381 aacggcaggt ggtcggtgct gcctggaccc aggcgatcgg ggtcaccccg gcggggaggg
     1441 acaaggcgac ggccagcatg cccgggttga ggtcctggcc gaccacctgg ccggtgaggc
     1501 cgacccggtc ggcggccagc cgggcgacga ccccggtgcc gcaggccgtg tccaggagcc
     1561 ggtcgcccgc ggccggggtg gcgaggtcga ggacccgggg ggcccagggg gcgaagatgg
     1621 ccgggacgag ctggccctgg tagacctcgg cggcgctgcc ggtgacctgc cagcgttcgt
     1681 cgcccatggc ggcgccgctc ctagcacggg agctgctgga gggcccagcc gttgccgtcg
     1741 gggtccttga agtaggcgta ggagacggtc ggcttgccgg gggcggtcat gtcctcgacc
     1801 tcgctgatct cgaccccccg ctccagcagc tcggcccggg tggcgtggat gtcggggacg
     1861 accaggtgga gggcctggac cgagccgggg ggtgtctgga cgatcccgac cccgatgcag
     1921 atggagcagg cggagccggg cggggtcagc tgcacgaccc ggaactcgtc gccgaagcgc
     1981 tcgtcgaggt cggcgtggaa gccgacctgg tcggtgtaga aggccttggc ccggtcgacg
     2041 tcggtcaccg ggatcggcac cagctcgagc ttcatggtca tggccggatg gtcctttgcg
     2101 aggtcgcggt cggggaaggt tatcctctcc ggtggccggg acagcgacaa ggagagccgg
     2161 cggggcgtga gcgagatcgg gaccgggacg ggcggggggc aacccctgga cgggctgcgg
     2221 gagcaggtgc gcgaggcggc cagggcggcg gccgcgcacg acctggcgcc caagccgccg
     2281 cgggcctcgg aggtcaccct gtcccagcac atggacctgt cgaacgccaa cttcatgggc
     2341 aacgtccacg gtggcgagat catgaagctg atcgacaccg ccgccgggat cgcggccagc
     2401 cgccacgccg gggggccggt ggtgacggcc tccctggacc agatgagctt cctgcacccg
     2461 gtgcacgtcg gcgacgtggt gctggtgcgg gcctcggtga acgacgtcgg gcggacctcg
     2521 atcgaggtcg gggtgcgggt cgaggccgag gagatcctca gcggccggcg cacccacacc
     2581 tcctcggcct acctggtgtt cgtggccctg gacgaacgcg ggcagccgcg gccggtgccc
     2641 gggctggtcc ccgagaacga cgtcgagcgc caccgccagg ccgaggccaa gatccgccgc
     2701 gagcaccgcc tggcccgggc cgaggccatc cggcgccggc gcgaccagct cgacacgtga
     2761 gcggggcgac ccggccgggc gacgacgggg cggtgacgcc ggcgcaggtg ctggccgggc
     2821 tgctgctggc catcatcgtg ctgtggctgc tggtcccgtt cgccggagtc gacccgccga
     2881 gggggctgac ggccagcaac gccccctgga ccgacgaggg cttcaacctg gccaacgccc
     2941 gcgagcgggt cctgaccggc cagttcgcca ccggcgacat cgaccgcagc ctgaccaacg
     3001 gcgcctacag cggcctggcg gcggccatgt tcgccgtgac cgggccgagc ctggccgccg
     3061 gccgggccct gtccatggtc gccgtcgccg cggccgtgct gctgctggcc ctcgggctgg
     3121 cccggccgct gggccgcccg gccgccctgc tcgccgccgc cgccctcggc ggggccaacc
     3181 tcatgctcga gcacggccgg ctcgccctgg tcgaggcgac cgtggtggcg ctgctggccg
     3241 gggcgttcgt gctcgccacc cgggcggcct ggcggccgtc gcccgccgcc gggatcgggc
     3301 tcggtctgct ggtcgcggcc gccgtgtcgg tcaaggcgat cgcgctcctg cccgggatgg
     3361 tgatgctggc cgtggtgctg gtcgcggccg ccgtccgccg ggaccggggg gcgctgggca
     3421 tgggcctggc cgggctggcg gccctggtcg cggccggcgg ggcctggctg gccgcggtgg
     3481 ccctgcccaa ctgggaccgg ttccggacgt cgctgcggat ctggccctcg gtcgagtacc
     3541 tggacgggcc ggccgcgatc gccggccgtc tcggccgcta cctggccgac tccgaccagg
     3601 cccttgggcg gagcctgccg ctgctgctgg ccgccgccct cggggtggcc gcgctggccc
     3661 gccgctggcc ccgcctcgct ccggaggcca gggacgcgct ggtcatggcc ctggtgtggg
     3721 ggaccgggct gtggctggcg gtggcggtgg ccgactacct gcccggcaac tacggcgccc
     3781 ccaaccgcta cgtcgtcccg gccctgcccg ggctggccgt cctggccggg ttcgggctgg
     3841 ccgacctggc cgccctcgcc ggccgggccc tgggccgaga gcgggccggg tgggcggccg
     3901 gggcggtcgg ggtcg
//
LOCUS       DASSWR010000619         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1153185, whole genome shotgun sequence.
ACCESSION   DASSWR010000619 DASSWR010000000
VERSION     DASSWR010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153185"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFO47_10345"
     CDS             <1..484
                     /locus_tag="VFO47_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8789136.1"
                     /translation="KAQPTAPVAGWTRFTSMQNHYNLIYREDEREMIPLCLDQGVGCI
                     PWSPLARGLLAGNRERGGAQRTVRAGTDPLADELYNNDDFDVVDVVRAVAAERGLPPA
                     RIALAWLLGKPAVTAPIVGATKLRHLEDAIAAVDVTLSEPEVARLEAAYRPHPVLGHH
                     "
     gene            637..1413
                     /locus_tag="VFO47_10350"
     CDS             637..1413
                     /locus_tag="VFO47_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8789137.1"
                     /translation="MDAVNPFPAERTAVITGAASPRGIGRATADRLARDGWSVAILDL
                     DGEAAKRTAAEIGGDRSVASLGVAADVADPDSVEVAIAQVEASLPPVIGLANVAGVSS
                     PVDFLDLTLAEWDRVFDINMRGAFLVTRRVVPAMLAAGVGRIVSVSSVSAQRGGGTYS
                     KVPYSASKAALIGFSRALARELSPHGITVNCVAPGPIDTDIMGGTLTQERKAELGADS
                     MVGRVGTVAEVAALMAFLLGEDAGYITAATYDINGGLQVS"
     gene            1418..>2251
                     /locus_tag="VFO47_10355"
     CDS             1418..>2251
                     /locus_tag="VFO47_10355"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET8789138.1"
                     /translation="MTPADPTANLPPARVEPDARRRIRRALADAGRRIAVLDDDPTGS
                     QTVHGVEVVTVVEPGELAAGLAEAGSTCFILTNTRSLAEADAVELNTRIGRLLFELAD
                     RLDAPIDVVSRSDSTLRGHVIAEVRALDAVRREATGRGFDGVLLAPAYFEAGRFTAGD
                     VHWARVGTDVLPVGETEFARDATFGYAASDLRDFVAERSGGTIGPDQVTSITLDDLRL
                     GGPARVAEVLAGVTGGGFVVVNAVEYADLEVVVGGLLEAEAAGRSFLCRTGPSFVQVL
                     AG"
BASE COUNT          297 a          837 c          822 g          295 t
ORIGIN      
        1 aaaggcgcag cccaccgccc cggtggccgg ctggacccgg ttcacgtcga tgcagaacca
       61 ctacaacctg atctaccgcg aggacgagcg ggaaatgatc ccgctctgcc tcgaccaggg
      121 cgtcggctgc atcccctgga gcccgctggc gcgcgggctg ctcgccggca accgggagcg
      181 aggcggcgcc cagcgcaccg tccgggccgg caccgacccc ctcgccgacg agctgtacaa
      241 caacgacgac ttcgacgtcg tcgacgtggt ccgcgcggtc gccgccgagc gtggcctgcc
      301 cccggcccgg atcgcgcttg cctggctgct cggcaagccc gccgtgaccg caccgatcgt
      361 cggcgcgacc aagctgcgcc acctcgagga cgcgatcgcc gcggtcgacg tgaccctgag
      421 cgagcccgag gtcgcccgcc tcgaggccgc ctaccgcccc cacccggtcc tgggccacca
      481 ctgaccgtca gacgagcgcc tgagtacagg ccgacgccga acggggaagc cacgacgtcg
      541 tcttgatggg aattggcctc ggctcgccgc tggctggtgg gtccgatcct cgggagaatg
      601 acggtcgcct gacctcgact ggaaggcagc aggcccatgg acgccgtcaa tccgttcccc
      661 gccgagcgca ccgcggtcat caccggggcc gcttccccgc gtggcatcgg gcgcgccacc
      721 gccgaccggc tggcccgcga cggctggtcg gtcgccatcc tcgacctcga cggcgaggcg
      781 gccaagcgga cagccgccga gatcggcggc gaccggtcgg tggcctcgct cggcgtcgcc
      841 gccgacgtcg ccgacccgga ctcggtcgag gtggccatcg cccaggtcga ggccagcctg
      901 ccgccggtca tcggcctggc caacgtggcc ggggtgagct ctccggtcga cttcctggac
      961 ctgaccctgg ccgagtggga ccgcgtcttc gacatcaaca tgcgcggcgc gttcctggtg
     1021 accaggcggg tcgtgcccgc catgctggcc gccggggtgg gccgcatcgt gagcgtctcg
     1081 tcggtctcgg cccagcgggg cggcggcacc tactccaagg tgccctacag cgcctccaag
     1141 gcggcgctca tcggcttcag ccgggccctg gcccgcgagc tgagcccgca cgggatcacg
     1201 gtgaactgcg tggcgccggg gccgatcgac accgacatca tgggcgggac cctgacccag
     1261 gaacgcaagg ccgagctggg cgccgacagc atggtcggcc gggtgggcac ggtggccgag
     1321 gtggccgccc tgatggcctt cctgctcggc gaggatgccg gctacatcac cgccgcgacc
     1381 tacgacatca acggcggcct ccaggtctcc taggtcgatg acaccggcgg acccgacggc
     1441 caacctgccc ccggcgcggg tggagccgga cgcccggcgg cggatccgcc gggccctggc
     1501 cgacgcgggc cggcggatcg cggtcctgga cgacgacccc acggggtccc agacggtcca
     1561 cggcgtcgag gtggtcaccg tggtcgagcc gggcgagctg gcggccggtc tcgccgaggc
     1621 gggctcgacc tgcttcatcc tgaccaacac ccgcagcctg gctgaggccg acgcggtgga
     1681 gctgaacacc cgcatcggac ggctgctgtt cgagctggcc gaccggctgg acgcgccgat
     1741 cgacgtggtc agccgcagcg actcgaccct gcgcgggcac gtcatcgccg aggtgcgcgc
     1801 gctcgacgcc gtgcgccggg aggcgaccgg ccgaggcttc gacggcgtgc tgctggcccc
     1861 ggcgtacttc gaggccggca ggttcaccgc cggggacgtg cactgggccc gggtgggcac
     1921 cgacgtcctg ccggtcggcg agaccgagtt cgcccgcgac gccaccttcg gctatgcggc
     1981 ctccgacctg cgcgacttcg tcgccgagag gagcggcggc acgatcgggc cggaccaggt
     2041 caccagcatc accctggacg acctccgcct gggcgggccg gcgcgggtgg ctgaggtcct
     2101 ggccggcgtg accggcggtg ggttcgtggt cgtgaacgcc gtggagtacg ccgacctgga
     2161 ggtcgtcgtc ggtgggctgc tcgaggccga ggcggccggc cggtccttcc tgtgccgcac
     2221 cgggccgtcg ttcgtgcagg tcctggccgg g
//
LOCUS       DASSWR010000620         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_757235,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000620 DASSWR010000000
VERSION     DASSWR010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757235"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /gene="glp"
                     /locus_tag="VFO47_10360"
     CDS             complement(<1..775)
                     /gene="glp"
                     /locus_tag="VFO47_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET8789139.1"
                     /translation="MAAVFPPDRPNPPDLVAFAEARAGVLERLGPLPVREAGLTDALG
                     CVLAADVAAAEDLPPFANSAMDGFAVRGADLAGAGEATPVPLRITGEVFAGTGQLPAV
                     EPGGAVRIMTGGALPPGADTVVPVEQTSVEGDTVLVRLDPGPGRFVREAGEDVRQGTV
                     VLERGRVLDPAAIGMLASVGRKVVPVHPRPRVTVVSTGDELVDPGDPLGPGQIRDSNS
                     WLLAAQARSAGAEAFRCGRLRDDPAVLRRGFALAAADGDL"
     gene            complement(785..1699)
                     /gene="galU"
                     /locus_tag="VFO47_10365"
     CDS             complement(785..1699)
                     /gene="galU"
                     /locus_tag="VFO47_10365"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET8789140.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAQPKEMLPLVDKPSIQYVIEEA
                     VAAGIEDILIVTGRGKQAIEDHFDRSVELEQLLTDKGDEDKLELVRHISDMAQVHYIR
                     QKAPLGLGHAVSVAEQHVGDEPFAVLLGDDVIDEGEKLLADLVTSFQRHGSSVVAAME
                     VERSQIKAYGCVAPADELEPGLFRMADIVEKPAPEEAPSNLAVIGRYVLHPEIFDALR
                     ATEPGAGNEIQITDALRHLVQSSERGVLAYAFTGTRYDAGDKNDYLRATVELAARRDD
                     IGPAFVAFLRRFVADLDRQAEARGPARH"
     gene            complement(1753..1875)
                     /locus_tag="VFO47_10370"
     CDS             complement(1753..1875)
                     /locus_tag="VFO47_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789141.1"
                     /translation="MNRLGETLSSTHDLPKLPGAHLLPLTARPGQVNGRAARVE"
BASE COUNT          316 a          905 c          889 g          310 t
ORIGIN      
        1 ccaggtcgcc gtcggcggcg gccagggcga agccgcggcg caggaccgcc gggtcgtcgc
       61 ggagccggcc gcagcggaac gcctcggccc cggccgagcg ggcctgggcg gccagcagcc
      121 aggagttcga gtcacggatc tggccgggac cgagcgggtc gccggggtcg accagctcgt
      181 cgccggtgga cacgaccgtg acccggggtc ggggatggac gggcacgacc ttgcggccga
      241 ccgaggcgag catgccgatg gccgccgggt ccaggacccg tccccgctcc agcaccaccg
      301 tgccctggcg gacgtcctcg ccggcctccc gcacgaaccg gcccgggccg gggtcgaggc
      361 ggaccagcac ggtgtcgccc tccaccgagg tctgctcgac cggcaccacc gtgtccgccc
      421 ccggcggcag cgccccgccg gtcatgatgc ggacggcgcc gcccggctcg acggcgggca
      481 gctggccggt cccggcgaag acctcgccgg tgatccgcag gggcaccggg gtcgcctcgc
      541 cggcgccggc caggtcggcc ccgcgcacgg cgaagccgtc catggccgag ttggcgaacg
      601 gtggcaggtc ctcggcggcg gccacgtcgg cggccaggac gcagccgagg gcgtcggtca
      661 gcccggcctc gcggaccggc agcgggccca accgctccag cacgcccgct cgcgcctcgg
      721 cgaacgccac caggtcgggc ggattcggcc ggtcgggcgg gaagacggct gccatggagt
      781 gatgctagtg ccttgccggc ccccgcgcct ccgcctggcg gtccaggtcg gccacgaacc
      841 ggcgcaggaa ggccacgaac gccgggccga tgtcgtcgcg gcgggcggcc agctcgaccg
      901 tggcccgcag gtagtcgttc ttgtcgccgg cgtcgtagcg ggtgccggtg aaggcgtagg
      961 cgagcacgcc ccgctccgag gactgcacga ggtgccggag ggcgtcggtg atctggatct
     1021 cgttgccggc cccgggctcg gtggcccgca gggcgtcgaa gatctccggg tggagcacgt
     1081 agcgcccgat cacggccagg ttcgaggggg cctcctcggg ggccggcttc tccacgatgt
     1141 cggccatccg gaacagcccg ggctcgagct cgtcggccgg ggcgacgcag ccgtaggcct
     1201 tgatctggga gcgctcgacc tccatggccg caaccacgct ggagccgtgc cgctggaagc
     1261 tggtcacgag gtcggccagc agcttctcgc cctcgtcgat gacgtcgtcg cccagcagga
     1321 cggcgaacgg ctcgtcgccg acgtgctgct cggcgacgct gacggcgtgg ccgaggccga
     1381 gcggcgcctt ctggcggatg tagtggacct gggccatgtc ggagatgtgg cggaccagct
     1441 ccagcttgtc ctcgtcgccc ttgtcggtga gcagctgctc cagctcgacg gagcggtcga
     1501 agtggtcctc gatggcctgc ttgccccggc cggtgacgat caggatgtcc tcgatgccgg
     1561 ccgcgaccgc ctcctcgatc acgtactgga tcgagggctt gtcgaccagc ggcagcatct
     1621 ccttcggctg ggccttggtc gccgggagga aacgggtccc cagcccggcc gctgggatga
     1681 cggccttgcg gaccttcatg atggctccac gatggcgatt ccccccgaag ttcgctgttc
     1741 agccggtaga ttctactcga cccgagcagc ccgtccgttg acctgccctg gccgcgcggt
     1801 cagcggcagc aggtgcgccc cgggcagctt gggcaggtcg tgggtcgagg acagggtctc
     1861 gcccaggcgg ttcatggacc tgggggcagc tcgtcgcggc tggatcgcag ggcgctcagg
     1921 tactcgccca gctcgacccg cagaagcggg ccgcgtcagg cggtcgagtc cacaaatcgg
     1981 caatctacgg gtgcgggcgg cgggcgcctg gttcatgatg gattggtacg ttcttcgccg
     2041 gcgggcagct ctgcctgaga caccatgcga agcgaggggg tgtcgtgccg tgcctggtga
     2101 ccggaaggcg tcgaagcgga aggcccgccc cgaacgcgaa cgcatcgagt acgcatccga
     2161 cgccatcggc tggctgcgcg agcagctcga gccgccgccc cagcagcccg ccgaccccaa
     2221 gcccatccaa ccggggccca aggcccccga gcgaccgagg caggccatgg ctcgcagaga
     2281 cgtccccccg cccgagccca ccccccctcc ccgggccgag ccaccggcgc ccgcgccgcc
     2341 tccgtcccca ccgcccgcgc ccgcgccgcc accaccggcc gcgccccctc ccccggccgc
     2401 gccgccgcct ccgccgccac
//
LOCUS       DASSWR010000621         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_21260,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000621 DASSWR010000000
VERSION     DASSWR010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21260"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..558
                     /locus_tag="VFO47_10375"
     CDS             316..558
                     /locus_tag="VFO47_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789142.1"
                     /translation="MLVRRLALDLRNLADKTLALRGVVEDYRHDLVRTLEDDWCDPDE
                     LQALHRQIQELWESMDRAEAKLRSGTRRMSPLLWLE"
     gene            complement(629..1786)
                     /locus_tag="VFO47_10380"
     CDS             complement(629..1786)
                     /locus_tag="VFO47_10380"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET8789143.1"
                     /translation="MTRIGVLTGGGDVPGLNPCIKTLVYAAADRGYEVVGLRRGWAGL
                     LDYNLDADTPQDHCVQPLTRHQVRTIDRTGGTFLHTSRTNPAKVKPTDVPAFLAGQGQ
                     FASPGPFDFTSHCLQVIERLGLDWLVAIGGDDTLSYAERLSREGVRTLAVPKTMDNDV
                     FGTDYCIGFSTAVTRSVQFIHQLRTSTGSHERIAVVELFGRNCGETSLLSAYLAGVDR
                     AIISEVPFDPELLAKYVLEDKASNPSRYAMVTISEGARMIHGAIEERGEADAYGHRKL
                     GGVGLVTGELLKQLTGQDIIYQQVGYLMRSGAPDALDLMVAVNFANMAINLIDQDAPG
                     SMVALRRGTYTSVPIDTMMSGTKRVDVDALYDDKRYRPLIRQVDGKPMFLY"
     gene            complement(1783..2193)
                     /locus_tag="VFO47_10385"
     CDS             complement(1783..2193)
                     /locus_tag="VFO47_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8789144.1"
                     /translation="MTRDVVLLASDMSAEIALRRLDQRAVSGAPVVDHGKVVGVITRR
                     DLLVPTLLDDPTGSLAAIAGHQHAPRLARLRVGELMSDEPVTAQPDWSVVLAVRTMID
                     HGVNRLPVVDQAGRPIGVLTRDDVLRAVARGVQA"
     gene            complement(2341..>3972)
                     /locus_tag="VFO47_10390"
     CDS             complement(2341..>3972)
                     /locus_tag="VFO47_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET8789145.1"
                     /translation="GTYRVSGDGYSPVGAVAPEGAAGPAGDALLERLARVAAACNDAT
                     LQPAGDPGGSWTVTGDPTEAALLALAGKLGVERDALDRAWPRVAEVAFDPTRRRMTTL
                     HQGPGQVWVAVKGAVEALVPLLAPADAALAGQVRAVANRWASDGYRILALAERDLPAV
                     PSRAEAAESGLRLLGLVAIADPPRAETAAAVAACREAGVIPVMITGDDARTATAIARR
                     VGILDEAGEILTGAELGYLDEETLAERVTGVGVYARTSPEQKLRLVGAWKDRGAVVAM
                     TGDGVNDAPALRQADIGVAMGVTGTDVSKEAADMVLADDNFATIVSAIAEGRRIYDNI
                     RRFVRYLLTTNSGEIWVMFLASALALPVPLLPVQILWINLVTDGLPAIALGLEPVERD
                     AMRRPPRSPTESIFARGLWQHALWVGLTMAAVCLALLVGARAAGWPWQTMVFATLALL
                     QLGHALAVRSERESFFTLGARSNPFLLAAVLGAVAVQLAILYLPALQRLFDTHPLSPF
                     QLALVLVTSTAAFVAVELEKWIIRRRSRRSRGSPA"
BASE COUNT          584 a         1414 c         1425 g          549 t
ORIGIN      
        1 ccccggcacc ctcccggccg agccctggcc cgagggccgc ctaccacacc gccatcgccg
       61 cctgctcgga cttccgggcc cgcctcgacg cggccccgcg gtccctgctc gagcgggccg
      121 ccgaggcggg gtcaccggcg cccgcctcgg cctgaatcgc cgttcaggca agggattggc
      181 ttgccaaggt cgccggcgaa taacatttct gtgattcctt ccccaacctt acgcaacgtg
      241 ctcccttcca gacggtatgc tccgttcgca ccatggctcg gaacggttgt ggagggtgat
      301 gcgatgacgg acccgatgct cgtccgacgg ctggccctcg acctccgaaa cctcgcggac
      361 aagaccctgg cgctgcgggg agtcgtcgag gactaccgcc acgatctcgt ccgcaccctc
      421 gaggacgact ggtgcgaccc ggacgagctt caggccctgc accgccagat ccaggagctg
      481 tgggagtcca tggaccgggc cgaggccaag ctgcgcagcg gcacccgccg catgtcgccg
      541 ctgctctggc tcgagtagcg cccggctcgg ccctccggcc agcacctcta cctcgtgccc
      601 agccccgacc cgggcagcta gggactgcct agtagaggaa catgggtttg ccgtcgacct
      661 ggcggatgag gggccggtag cgcttgtcgt cgtacagggc gtcgacgtcg acgcgcttgg
      721 tgcccgacat catggtgtcg atcgggaccg aggtgtaggt gccccggcgc agggcgacca
      781 tgctccccgg ggcgtcctgg tcgatcaggt tgatggccat gttggcgaag ttgaccgcga
      841 ccatcaggtc cagggcgtcg ggggcgcccg agcgcatcag gtagccgacc tgctggtaga
      901 tgatgtcctg gccggtgagc tgcttgagca gctcgccggt gaccaggccg accccgccca
      961 gcttgcggtg gccgtaggcg tcggcctcgc cccgctcctc gatggcgccg tggatcatcc
     1021 gggcaccctc gctaatggtc accatggcgt agcgggacgg attcgaggcc ttgtcctcca
     1081 ggacgtactt cgccaggagc tccggatcga acggcacttc ggagatgatg gctcggtcga
     1141 cgccggccag gtaggccgag agcagcgagg tctcgccgca gttgcggccg aacagctcga
     1201 ccacggcgat gcgctcatgg gagccggtcg aggtccgcag ctggtggatg aactgcacgc
     1261 tgcgggtgac ggcggtcgag aacccgatgc agtagtcggt gccaaagacg tcgttgtcca
     1321 tcgtcttggg cacggccagg gtccgcaccc cctcccggga cagccgctcg gcatacgaca
     1381 aggtgtcgtc gccgccgatg gcgaccagcc agtccagccc cagacgctcg atcacctgga
     1441 ggcagtggct ggtgaagtcg aaggggcccg gggaggcgaa ctggccctgg ccggccagaa
     1501 acgccggcac gtcggtgggt ttgaccttgg ccgggttggt ccggctggtg tgcaggaagg
     1561 tcccaccggt ccggtcgatg gtgcggacct ggtggcgggt gagcggctgg acgcagtggt
     1621 cctggggggt gtcggcgtcc aggttgtagt cgagcagccc ggcccagccg cgccgcagcc
     1681 caaccacctc atagccccgg tcggcggcgg cgtagaccag ggtcttgatg caggggttga
     1741 gaccggggac gtcgcccccg ccggtgagca caccgatcct ggtcatgcct ggaccccccg
     1801 cgccaccgcc ctcagcacgt cgtcgcgggt caggaccccg atcgggcgtc cggcctggtc
     1861 gacgaccggc agccggttga cgccatggtc gatcatcgtc cgcaccgcca gcaccaccga
     1921 ccagtccggc tgggcggtca ccggctcgtc gctcatcagc tcgccgaccc tgagacgggc
     1981 caggcggggc gcgtgctggt gcccggctat cgcggccagg gagccggtgg ggtcgtccag
     2041 cagcgtcggc acgagcaggt cacggcgggt gatcaccccc acgaccttgc cgtggtcgac
     2101 gaccggcgcc ccggacacgg ccctctggtc gaggcgtcgc agggcgatct cggccgacat
     2161 atccgatgcc agcagcacca cgtcccgggt catggcctcg cccacggtac ccaactgccg
     2221 ccccagaatg gtcgattcct ccgcggtcgc ggtcacggcc atcacctccc aagtggcggg
     2281 cgtacctcac ccgcccagga gatcagcctg cccggtcaat gagcgaccat aggagggcca
     2341 tcaggcaggt gaaccacggg accttcggga ccgccggcgg atgatccact tctcgagctc
     2401 taccgcgacg aaggcggcgg tcgaggtcac caggacgagg gcgagctgga acggcgagag
     2461 ggggtgggtg tcgaacagcc gctgcagggc cggcaggtag aggatggcga gctggaccgc
     2521 gaccgcccca agaaccgccg ccagcaggaa cgggttggac cgggcgccca gggtgaagaa
     2581 gctctcgcgc tcggaccgca ccgccagggc gtggcccagc tgcagcaggg ccagggtggc
     2641 gaacaccatc gtctgccacg gccagccggc cgccctggcg ccgaccagga gagccaggca
     2701 gacggcggcc atggtcagcc cgacccacag ggcgtgctgc cacaggccgc gggcgaagat
     2761 cgactcggtc ggcgaccggg gtgggcggcg catggcgtcg cgttccaccg gctccagccc
     2821 gagcgcgatg gcgggcagac cgtcggtgac caggttgatc cagaggatct gcaccggcaa
     2881 cagcgggacg ggcagggcaa gcgcggaggc caggaacatg acccagatct cgcccgagtt
     2941 ggtggtgagc aggtagcgca cgaagcggcg gatgttgtcg tagatccgcc gcccctcggc
     3001 gatggcggag acgatggtgg cgaagttgtc gtcggccaga accatgtcgg ccgcctcctt
     3061 ggagacgtcg gtgccggtca cgcccatggc caccccaatg tcggcctggc gcagggccgg
     3121 ggcgtcgttg acgccgtcgc cggtcatcgc caccaccgca ccccggtcct tccaggcacc
     3181 gaccagccgg agcttctgct ccgggctggt ccgggcgtac acgcccacgc cggttactcg
     3241 ctcggccagg gtctcctcgt ccaggtagcc cagctcggcc ccggtgagga tctcgccggc
     3301 ctcgtcgagg atgccgacgc ggcgggcgat ggcagtggcc gtgcgggcat catctccggt
     3361 gatcatcacc gggatgacgc cggcctcccg gcaggcggcc acggcggcgg ccgtctcggc
     3421 ccgtggcggg tcggcgatgg ccaccagccc gaggaggcgc aggccgctct cggccgcctc
     3481 ggcccggctg ggcaccgcgg gcaggtcccg ctcggccagg gccaggatgc ggtaaccgtc
     3541 ggacgcccac cggttggcca ccgcccgcac ctgcccggcc agcgcggcgt cggccggggc
     3601 gagcagcggg accagggcct cgaccgcccc cttgaccgcc acccacacct ggcctggccc
     3661 ctggtgcagc gtggtcatgc gccgccgggt ggggtcgaag gcgacctcgg ccacccgggg
     3721 ccaggcgcgg tccagcgcat cacgctcgac gcccagcttg ccggccaggg ccagcagagc
     3781 ggcctcggtc gggtcgccgg tcacggtcca ggagccgccc gggtcgccgg ccggctgcag
     3841 cgttgcgtcg ttgcaggccg ccgccaccct ggccaggcgc tccagcagcg cgtcgccggc
     3901 cggtccggcg gcgccctcgg gcgcgacggc accaaccggc gagtatccgt cgccgctgac
     3961 ccgataggtc cc
//
LOCUS       DASSWR010000622         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_165465,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000622 DASSWR010000000
VERSION     DASSWR010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165465"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFO47_10395"
     CDS             <1..681
                     /locus_tag="VFO47_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET8789146.1"
                     /translation="CANGLLHAAADAAGPGALQLGDGVRGRHVLVTGAGVVGLLVALL
                     ARHHGAAEVVVADATPVRLAAARALGLGTVDDADGTAWRTVKERWRRDPTDCGADVVF
                     QCRGRTAALAGALRSLRPQGTVVDLAFYQGGAPDLALGEEFHHNGLTIRCAQIGRVPR
                     GLGHLWDRRRLAAETVDLLRASGPAMREHLVTDLVPLGDAPALLADLASRRRHVLQAV
                     FACPAATA"
     gene            705..1751
                     /locus_tag="VFO47_10400"
     CDS             705..1751
                     /locus_tag="VFO47_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8789147.1"
                     /translation="METSDWVVVGIDNGGTSNNATILDASGRFLVDRLVEVPSRVTEG
                     PEVAIDAMAGAMDNVLDLTGVPLSAVRGVGLDTPGPASADGVISSKGATNFAQPAWRG
                     FDVRGGLEARLGLPVVYNNDANAAALYAHHVRFRDKAAGHSSVSAIVGTGLGGGVVES
                     GRVVRGAAGMAGELGHVQIPMHGLLAEGQPRPTCNCGFVGDAESIASLTGIERNLLPY
                     WLTRFENHELGRIADRREAAKLVRGYGEREDPLALAIFEQQAMAIGRLFTIAANFTDP
                     DTYFIGGGVVETIPKFRQWFLAKVREHTALREEQARVATLALVPDLDMAGARGAAIAA
                     LDALQAPVRARTSL"
     gene            complement(1718..2860)
                     /locus_tag="VFO47_10405"
     CDS             complement(1718..2860)
                     /locus_tag="VFO47_10405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8789148.1"
                     /translation="MSLTVLVNAGPWLPVPPPDYGGIENVLATLIPELRFRGVEVVLC
                     TVEESTVEVDDRRCAFPDGQFGRLAGPYAQVMGITHAHMATVLETLRDRPEIDLVHDH
                     LEVVGPSVLGAIDGGGPPVLQTLHWDLRKHPEFYGSFDGKGRVFFNGVSNAQLATAPA
                     NLREQSLGAVHLGVDLDAHHFRADKGEDFLVLGRVTPFKGQDVAARICKELGVPLVMA
                     GPVAGVPTPKELSAALEDPASPLHGYGDVRHYLDAVQPFEDGERIRWVGTVGGAAKDE
                     LVGRARAVLMPISWEEPGATAAIEALACGTPVIATRRGALPEIIEHGHNGFLADDEAD
                     FAQLMLRAGEIDPAACRRSVEERFSAGTMAEGYLRLYREVLARTGA"
     gene            complement(2932..3300)
                     /locus_tag="VFO47_10410"
                     /pseudo
     CDS             complement(2932..3300)
                     /locus_tag="VFO47_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
BASE COUNT          437 a         1279 c         1264 g          442 t
ORIGIN      
        1 tgtgccaacg gcctgctgca cgccgccgcc gacgccgccg ggccgggcgc gctccagctc
       61 ggcgacggcg tccggggccg ccacgtgctg gtcaccgggg ccggcgtggt cggcctgctg
      121 gtcgccctcc tggcccgcca ccacggcgcc gccgaggtgg tggtggccga cgccaccccg
      181 gtccggctgg ccgcagcgag ggccctgggg ctggggacgg tcgacgacgc cgacgggacc
      241 gcctggcgga cggtcaagga gcgctggcgc cgcgacccca ccgactgcgg tgccgacgtc
      301 gtcttccagt gccggggccg gaccgccgcc ctggccggcg ccctgcgcag cctccggccc
      361 cagggcacgg tcgtcgacct ggccttctac cagggcgggg cgcccgacct ggccctgggc
      421 gaggagttcc accacaacgg gctgaccatc cgctgcgctc agatcggccg ggtgccccgg
      481 ggcctgggcc acctgtggga ccggcgccgc ctggccgccg agacggtcga cctgctgcgt
      541 gcatccgggc cggcgatgcg cgagcatctc gtcaccgacc tggtcccgct cggcgacgcc
      601 ccggccctgc tggccgacct ggccagccgc cgtcgccacg tcctccaggc ggtgttcgcc
      661 tgcccggcgg cgacggccta gaccagccgg aggagggagc ggagatggag accagcgact
      721 gggtcgtcgt cggcatcgac aacggcggca ccagcaacaa cgccacgatc ctcgacgcgt
      781 cggggcgttt cctggtcgac cgcctggtcg aggtgccgag ccgggtcacc gagggccccg
      841 aggtggccat cgacgccatg gccggcgcca tggacaacgt gctcgacctc accggcgtgc
      901 cgctgtcggc ggtccgcggg gtggggctgg acacgcccgg cccggcctcg gccgacgggg
      961 tcatctcctc caagggcgcg accaacttcg cccagccggc ctggcggggc ttcgacgtcc
     1021 ggggcggcct ggaggcgcgg ctggggctgc cggtggtcta caacaacgac gccaacgcgg
     1081 ccgccctgta cgcccaccac gtccgcttcc gggacaaggc ggccggccac tcgtcggtgt
     1141 cggcgatcgt cggcaccggc ctgggcggcg gggtggtcga gagcgggcgg gtggtccgcg
     1201 gggccgccgg catggccggc gagctcggcc acgtccagat ccccatgcac ggcctgctgg
     1261 ccgagggcca gccgcggccg acctgcaact gcggctttgt cggcgacgcc gagagcatcg
     1321 cctccctgac cgggatcgag cgcaacctcc tcccctactg gctgaccagg ttcgagaacc
     1381 acgagctggg ccggatcgcc gaccggcggg aggcggccaa gctggtccgc ggctacggcg
     1441 agcgggagga cccgctggcc ctggccatct tcgagcagca ggcgatggcc atcggccggc
     1501 tgttcaccat cgccgccaac ttcaccgacc ccgacaccta cttcatcggc ggcggggtgg
     1561 tcgagaccat cccgaagttc cgccagtggt tcctggccaa ggtgcgcgag cacaccgccc
     1621 tgcgcgagga gcaggcccgg gtggccaccc tggccctggt ccccgacctg gacatggccg
     1681 gcgcccgcgg ggccgccatc gccgccctgg acgcgcttca ggcgccggtc cgggccagga
     1741 cctccctgta gaggcgcagg tagccctcgg ccatcgtccc ggccgagaac cgctcctcca
     1801 ccgaccgccg gcaggcggcc gggtcgatct cgccggcgcg cagcatgagc tgggcgaagt
     1861 cggcctcgtc gtcggccagg aagccgttgt ggccgtgctc gatgatctcg ggcagggcgc
     1921 cccggcgggt cgcgatcacc ggggtgccgc aggccagcgc ctcgatcgcc gccgtcgccc
     1981 ccggctcctc ccagctgatc ggcatgagca cggccctggc ccggccgacc agctcgtcct
     2041 tggccgcccc gccgaccgtg cccacccagc ggatccgctc gccgtcctcg aacggctgga
     2101 cggcgtccag gtagtggcgg acgtcgccgt agccgtgcag cgggctggcc gggtcctcga
     2161 gggccgcgga cagctccttc ggggtgggga cgccggcgac cgggccggcc atgaccagcg
     2221 gcaccccgag ctccttgcag atgcgggcgg ccacgtcctg gcccttgaac ggggtgaccc
     2281 ggccgaggac caggaagtcc tcgcccttgt cggcccggaa atggtgggcg tccaggtcga
     2341 cgccgaggtg gacggcgccg aggctctgct cccggaggtt ggccggggcg gtggcgagct
     2401 gggcgttgga gacgccgttg aagaacaccc ggcccttgcc gtcgaaggag ccgtagaact
     2461 cgggatgctt gcgcaggtcc cagtggaggg tctggagcac cggcgggccg ccgccgtcga
     2521 tcgccccgag cacgctcggc cccaccacct ccaggtggtc gtggaccagg tcgatctcgg
     2581 gccggtcgcg cagcgtctcc aggacggtgg ccatgtgggc gtgggtgatg cccatgacct
     2641 gggcgtacgg gccggccagg cgcccgaact ggccgtcggg gaaggcgcag cggcggtcgt
     2701 cgacctccac cgtgctctcc tcgacggtgc acagcaccac ctcgaccccc cggaagcgca
     2761 gctccgggat cagggtggcg agcacgttct cgatcccgcc gtagtccggc ggcggcaccg
     2821 gcagccaggg accggcgttg acgagcacgg tgagcgacat gggatcccgc aatttcggtt
     2881 gttccggacc cgccccatgt tgccaccgtc cggctgcccc aaacctatcg ttcaggcgcg
     2941 ggggaaccgg agcacgcggt cgtcgtcggc ggcggggtcg ccgcggccgt cgcggttgct
     3001 ggtcaggacc cagagggcgc cgtcggcggc cacggcggcg tggcgcagcc ggccgtagga
     3061 cccggccagc acggccgccg gctcgccggt cccgccgtcg ccgtccaggg gcaccgtcca
     3121 cagccgctcg ccccgcaggg cggccacgta cagggtgccg ccggcgatcg cggccccgct
     3181 gggcgacgcc tgcgaggtcg gccaggtgac cagcgggtcg cggaaccgtc ctccgccgcc
     3241 ggtcccctcg acctccggcc agccgtagtt gccgccccgc tcgatccggt tgatcttatc
     3301 caaccggcgg tattatgact gaaggtagca acgggcgacg aagggcacgc gatgaccggg
     3361 aaccgaattc gagccgcgat ctatgttcgg atttccaagg atcgaaccgg cgaagcgctt
     3421 gg
//
LOCUS       DASSWR010000623         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_249706,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000623 DASSWR010000000
VERSION     DASSWR010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249706"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..852
                     /locus_tag="VFO47_10415"
     CDS             61..852
                     /locus_tag="VFO47_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET8789149.1"
                     /translation="MTDRSPALTYSSYLALDELLAAQRPRSDEHDEMLFIVVHQVYEL
                     WFKQLLHELAHLQDRLAAGDTAHALHTLKRVLTVLKVAVAQVDVLETMTPRQFLGFRD
                     RLDRASGFQSAQFRELEAVLGRRDRSVLDAYAPDSLERARIEAAMERPSLFDSFLRYL
                     HVRGYDVPRAVLDRHVGRRWRPDPEVQEVLLQVHRDDGGPAMVAERLVDLDEGLQEWR
                     YRHVKMVERTIGTRGGTGGSSGADYLRSTLWQPMFPDLWAIRSRL"
     gene            849..2066
                     /locus_tag="VFO47_10420"
     CDS             849..2066
                     /locus_tag="VFO47_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET8789150.1"
                     /translation="MITAEDLAASPNPLARHYRRFRVEERVLLTGHSHQAWPDVAAEG
                     QLAALEDAAALVDDKWDRALAVADEVRAGFRRLLADPGAEIALGASTHELVVRLLSAL
                     DLGGTRPRLVSTDGEFHSLRRQLARLAEGGVEVVLVPAEPAGTLAERLAAAVDDRTAA
                     VLVSAVLFASARIVPHLDGLAAACERRGAELVVDAYHALGPVPLPVHELGLGSAWVTG
                     GGYKYLQLGEGNCFLRLPPQADRFRPVVTGWFAEFGELAAHHDPGRVAYPRGAGRFAG
                     ATYDPTSHYRAARVLRFFAEQGLTPELLRRSYQRQLAVLAAGFDALGVDDRLATRDRD
                     APLDALGGFLALRTPHAARLQQALRDRGVATDSRGDVLRLGPAPYLSDAQLEAGIAAL
                     GQALADPALPPAT"
     gene            2095..2508
                     /locus_tag="VFO47_10425"
     CDS             2095..2508
                     /locus_tag="VFO47_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HET8789151.1"
                     /translation="MTDAAPPPSPVFLYDGDCSFCTSCATFIERRIPTSARVVPWQFA
                     DLDALGVTQAQAEEAVVWVAPGEPTRHGPEAIARMLVDAGSYWRPLGRLLGLRPVLWV
                     AWPVYRWVARNRHRMPGGTAACSRPQAEREARRPA"
     gene            complement(2412..>2756)
                     /locus_tag="VFO47_10430"
     CDS             complement(2412..>2756)
                     /locus_tag="VFO47_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HET8789152.1"
                     /translation="QAQAEEAVVWVAPGEPTRHGPEAIARMLVDAGSYWRPLGRLLGL
                     RPVLWVAWPVYRWVARNRHRMPGGTSEPGPRATGNRSRAQAGRRASRSAWGREQAAVP
                     PGIRCRLRATHR"
BASE COUNT          293 a         1102 c         1019 g          342 t
ORIGIN      
        1 gctccggccg gcgcccgtcg acggccggcg gccgccggcc ggaccccggg aggtgaggcc
       61 atgaccgacc gctcgcccgc cctcacctac tcgtcgtacc tcgcgctcga cgagctgctc
      121 gccgcccagc gcccccgctc ggacgagcac gacgagatgc tgttcatcgt cgtccaccag
      181 gtctacgagc tgtggttcaa gcagctcctg cacgagctgg cccacctgca ggaccggctg
      241 gcggcggggg acacggcgca cgcgctccac acgctgaagc gggtgctgac ggtgctgaag
      301 gtcgccgtcg cccaggtcga cgtcctggag acgatgacgc cccgccagtt cctcggcttc
      361 cgcgaccggc tcgaccgggc gagcgggttc cagtcggccc agttccgcga gctcgaggcc
      421 gtgctcgggc ggcgggaccg cagcgtgctc gacgcctacg ccccggactc gctcgagcgg
      481 gcccggatcg aggcggcgat ggagcggccg tcgctgttcg actcgttcct ccgctacctg
      541 cacgtccggg gctacgacgt gccccgggcg gtgctcgacc gccacgtcgg ccggcgctgg
      601 cgccccgacc ccgaggtcca ggaggtgctg ctgcaggtcc accgggacga cggcgggccg
      661 gcgatggtgg ccgagcggct ggtcgacctc gacgagggcc tgcaggagtg gcgctaccgc
      721 cacgtgaaga tggtcgagcg gaccatcggc acccgcggcg gcaccggcgg ctcctccggc
      781 gccgactacc tccgctcgac cctgtggcag ccgatgttcc ccgacctgtg ggccatccgg
      841 agccggctgt gatcaccgcc gaggacctcg cggcgtcgcc caacccgctg gcccggcact
      901 accgccggtt ccgggtggag gaacgggtgc tgctcaccgg ccactcccac caggcgtggc
      961 cggacgtggc cgccgagggc cagctcgccg ccctggagga cgccgccgcg ctggtcgacg
     1021 acaagtggga ccgggccctc gcggtcgccg acgaggtgcg cgcggggttc cggcggctgc
     1081 tcgccgaccc cggggcggag atcgccctgg gggccagcac gcacgagctg gtcgtgcgcc
     1141 tgctctccgc cctcgacctc ggcggcaccc gcccccggct cgtgtcgacc gacggcgagt
     1201 tccacagcct gcgccgccag ctcgcccggc tggccgaggg cggcgtcgag gtcgtgctcg
     1261 tgcccgccga gccggccggc acgctggccg agcggctggc cgccgcggtc gacgaccgca
     1321 ccgccgccgt gctggtctcc gccgtgctgt tcgcctcggc ccgcatcgtg ccccacctcg
     1381 acgggctggc cgcggcgtgc gagcggcggg gcgccgagct ggtcgtcgac gcgtaccacg
     1441 cgctggggcc ggtcccgctg ccggtgcacg agctgggcct gggctcggcg tgggtcaccg
     1501 gcggcggcta caagtacctg cagctcggcg agggcaactg cttcctgcgc ctgccgccgc
     1561 aggccgaccg gttccggccc gtcgtcaccg gctggttcgc cgagttcggt gagctggccg
     1621 cccaccacga ccccggccgg gtcgcctacc cgcgcggcgc cggccgcttc gccggcgcca
     1681 cctacgaccc gaccagccac taccgggcgg cccgggtgct gcggttcttc gccgagcagg
     1741 ggctgacccc cgagctgctg cgccgctcgt accagcgcca gctcgccgtg ctcgcggccg
     1801 gcttcgacgc gctgggggtg gacgaccggc tggccacccg cgaccgcgac gcaccgctgg
     1861 acgcgctcgg cggcttcctc gcgctgcgga cgccgcacgc ggcccgcctg cagcaggcgc
     1921 tgcgggaccg gggcgtcgcc accgacagcc gtggcgacgt gctgcggctg ggacccgcgc
     1981 cctacctgtc ggacgcccag ctcgaggcgg gcatcgccgc cctcggccag gcgctggccg
     2041 acccggccct gccgccggcg acctgatccg gccgccggcc gccacaatgg cggcgtgacc
     2101 gacgccgcgc ccccgccgtc cccggtgttc ctctacgacg gcgactgctc cttctgcacc
     2161 agctgcgcga ccttcatcga gcgccgcatc cccacctccg cccgggtggt gccctggcag
     2221 ttcgccgacc tcgacgcgct cggggtcacg caggcccagg ccgaggaggc ggtcgtgtgg
     2281 gtggcgcccg gcgagcccac ccgccacggc cccgaggcga tcgcccggat gctggtcgac
     2341 gccggctcgt actggcgccc cctcggccgg ctgctggggc tgcggccggt gctgtgggtg
     2401 gcgtggcccg tctaccggtg ggtggcccgc aaccggcacc ggatgccggg gggcacggcg
     2461 gcctgctcgc ggccccaggc cgagcgcgag gcccggcgcc ccgcctgagc gcgcgaccgg
     2521 ttgcccgtcg cgcgcgggcc tggttcactg gtgccgccgg gcatccggtg ccggttgcgg
     2581 gccacccacc ggtagacggg ccaggccacc cacagcaccg gccgcaggcc cagcagccgg
     2641 ccgagggggc gccagtacga gccggcgtcg accagcatgc gggcgatcgc ctcggggccg
     2701 tggcgggtgg gctcgccggg cgccacccac acgaccgcct cctcggcctg ggcctg
//
LOCUS       DASSWR010000624         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1125208, whole genome shotgun sequence.
ACCESSION   DASSWR010000624 DASSWR010000000
VERSION     DASSWR010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125208"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFO47_10435"
     CDS             complement(<1..303)
                     /locus_tag="VFO47_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8789153.1"
                     /translation="MEPSDWVVVGIDNGGTSNNATILDASGRFLVDRLVEMPSRVTEG
                     PEVAIDAMAGALDNVLDLTGVPLSAVRGVGLDTPGPASADGVISSKGATNFAQPAWR"
     gene            complement(327..1442)
                     /locus_tag="VFO47_10440"
     CDS             complement(327..1442)
                     /locus_tag="VFO47_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET8789154.1"
                     /translation="MADRRPVRLLVIERPGVARLVDEDEPAPAGGQFRLETLYTGLSA
                     GTELTFFKGTNPYLHAAWDENLGVFRRDRPATGFPVRAPGYMEVGRVAETSGDAVAVG
                     DLVAMAYGHKTGHTVDPATDRFVPLPPDLDPVLGIYVAHMGPICANGLLHAAADAAGP
                     GALQLGDGVRGRHVLVTGAGVVGLLVALLARHHGAAEVMVADATPERLTAARALGLDA
                     VDDADGTAWRTVKERWRRDPTDCGADVVFQCRGRTAALAGALRSLRPQGTVVDLAFYQ
                     GGAPDLALGEEFHHNGLTIRCAQIGRVPRGLGHLWDRRRLAAETVDLLRASGPAMREH
                     LVTDLVPLGDAPALLADLASRRRHVLQAVFACPAATA"
     gene            complement(1435..2436)
                     /locus_tag="VFO47_10445"
     CDS             complement(1435..2436)
                     /locus_tag="VFO47_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8789155.1"
                     /translation="MLPTVRIGMVGAGAVAARHVRTLLAMDGVEVAAVADPALERAKE
                     LAGEAGAATYPNHMELLASERLDAVYICVPPFAHGAPELAVIDAGLPMFVEKPVAIDQ
                     ETAAAIAARLAGRPLVTCTGYHWRWLDIFDRAADLLADRPARLVHCSWLDKIPPPPWW
                     RRRDGSGGQTIEQTTHVLDTARALAGDVAEVHAFGSRAAATQDQGSEAALPAADIDEV
                     SVASLRFASGAVGSVTSTCLLPRLHRAGVHVVADGLALELSETALVVEEDGRRDAWDA
                     DADARPRPDRDFVAAVRGGEDRIRVPWPEAYRTHLLACAITRSAEEGRPLAVEQHDG"
BASE COUNT          327 a         1007 c         1027 g          319 t
ORIGIN      
        1 ccgccaggcc ggctgggcga agttggtcgc gcccttggag gagatgaccc cgtcggccga
       61 ggccgggccg ggcgtgtcca gccccacccc gcggaccgcc gacagcggca cgccggtgag
      121 gtcgagcacg ttgtccaggg cgccggccat ggcgtcgatg gccacctcgg ggccctcggt
      181 gacccggctc ggcatctcga ccaggcggtc gaccaggaac cgcccggacg cgtccaggat
      241 cgtggcgttg ttgctggtgc cgccattgtc gatgccgacg acgacccagt cgctgggctc
      301 catctccgct ccctcctccg gctggtctag gccgtcgccg ccgggcaggc gaacaccgcc
      361 tggaggacgt ggcgacggcg gctggccagg tcggccagca gggccggcgc gtcgccgagc
      421 gggaccaggt cggtgacgag atgctcgcgc atcgccggcc cggatgcacg cagcaggtcg
      481 accgtctcgg cggccaggcg gcgccggtcc cacaggtggc ccaggccccg gggcacccgg
      541 ccgatctggg cgcagcggat ggtcagcccg ttgtggtgga actcctcgcc cagggccagg
      601 tcgggcgccc cgccctggta gaaggccagg tcgaccacgg tcccctgggg ccggaggctg
      661 cgcagggcac cggccagggc ggcggtccgg ccacggcact ggaagacgac gtcggcgccg
      721 cagtcggtgg ggtcgcggcg ccagcgctcc ttgaccgtcc gccaggcggt cccgtcggcg
      781 tcgtcgaccg cgtccaggcc gagggcccgg gcagcggtca gccgctccgg ggtggcgtcg
      841 gcgaccatca cctcggcggc gccgtggtgg cgggccagga gggcgaccag caggccgacc
      901 accccggccc cggtgaccag cacgtggcgg ccccggaccc cgtcgccgag ctgcagcgcc
      961 cccggcccgg cggcgtcggc ggcggcgtgc agcagcccgt tggcgcagat cgggcccatg
     1021 tgggcgacgt agatgccgag caccgggtcc aggtcgggcg gcaggggcac gaaccggtcg
     1081 gtggccgggt cgacggtgtg gccggtcttg tggccgtagg ccatggccac caggtcgccc
     1141 acggccacgg cgtcgccgct ggtctcggcg acccggccga cctccatgta gccgggggcg
     1201 cggaccggga agccggtcgc cggccggtcg cgccggaaca cccccaggtt ctcgtcccag
     1261 gcggcgtgca ggtaggggtt ggtgcccttg aagaaggtca gctcggtccc ggccgacagc
     1321 ccggtgtaga gggtctccag gcggaactgg ccgccggccg gggccggctc gtcctcgtcc
     1381 accagccggg ccaccccggg ccgctcgatc accagcagcc gcaccggccg gcggtcagcc
     1441 atcgtgctgc tccacggcca gcggccggcc ctcctcggcc gagcgggtga tggcgcaggc
     1501 cagcaggtgg gtccggtagg cctcgggcca cgggacccgg atccggtcct ccccgccccg
     1561 gaccgcggcc acgaagtccc ggtccggccg gggccgcgcg tcggcgtcgg cgtcccaggc
     1621 gtcgcgccgg ccgtcctcct ccaccaccag cgcggtctcg gacagctcca gggcgaggcc
     1681 gtcggcgacc acgtgcaccc ccgcccggtg cagccgcggc agcaggcagg tcgaggtcac
     1741 gctccccacc gcccccgagg cgaaccggag gctggccacc gacacctcgt cgatgtccgc
     1801 agccggcagc gccgcctccg agccctggtc ctgggtggcg gctgcccggg aaccgaaggc
     1861 gtggacctcg gccacgtcgc cggcgagggc gcgggcggtg tcgaggacgt gggtggtctg
     1921 ctcgatcgtc tggccgcccg acccgtcgcg gcgccgccac caggggggtg gggggatctt
     1981 gtccagccag gagcagtgga cgaggcgggc cggccggtcg gccagcaggt cggccgcgcg
     2041 atcgaagatg tccagccagc gccagtggta gccggtgcag gtgaccagcg gccggccggc
     2101 caggcgggcg gcgatggccg cggccgtctc ctggtcgatc gcgaccggct tctccacgaa
     2161 catgggcagg ccggcgtcga tcacggccag ctcgggggcc ccgtgggcga acggcgggac
     2221 gcagatgtag acggcgtcga ggcgctcgga ggccagcagc tccatgtggt tggggtaggt
     2281 ggccgcgccg gcctcaccgg ccagctcctt ggcccgctcc agggccgggt cggcgaccgc
     2341 ggccacctcg accccgtcca tggccagcag cgtccgcacg tggcgggccg cgaccgcgcc
     2401 cgccccgacc atgccgatcc gtaccgtagg cagcatccca gcaggatagg gccaaccgga
     2461 cggaccggca gcgacccgac ccggaccggg gtgcctctgg tagcctcctg tcgccgtctg
     2521 gtccacttca gcgtgcttcc ccaggagatc cgtgtggctg acatcgacgc cttcgtccag
     2581 gccgaggcgg acgggctcgt gcatgagctg tccgagtggt gcgccatccc ctccatctcc
     2641 agcgaccccg agcgcgccgg cgacgtccgc cgctcggccg
//
LOCUS       DASSWR010000625         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1323440, whole genome shotgun sequence.
ACCESSION   DASSWR010000625 DASSWR010000000
VERSION     DASSWR010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323440"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFO47_10450"
     CDS             <1..627
                     /locus_tag="VFO47_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET8789156.1"
                     /translation="EIDLGKGRVRRLLKTVRTPRGLWPTADGRRLYVAGFENGDLQRI
                     DLASGKAKTLLRTGGAMRHLVGDPQGRRLYADDMGTNQAFVVDLANERVRRLAGTDNV
                     PNTIDLTPDGRVLYVSNRGRNGASYYNPGPEWGSVLAIDTASGKVLDAMVGGNQTTGL
                     DVSADGRLLAFSDFLDNRIHIYAVPPYAALAAGGGGRAAAHRAELAKR"
     gene            633..1361
                     /locus_tag="VFO47_10455"
     CDS             633..1361
                     /locus_tag="VFO47_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8789157.1"
                     /translation="MRRLVLWDIDGTLVQAGEVGRDIFTEAFQAVVGRAPDQVAAKAL
                     AMAGRTDPEIALEFLAAHEIAEGATHLPAFSEALVTALAAKAAVIRERGRALPGARET
                     LAALERTDGVVQSLLTGNVQPNALLKLASFELDGYLDFDVGGFGSDHHHRPSLVEVAR
                     AKAEAKYGTAFEGTATVLVGDTPLDVAAGRAGGARVVAVATGPYRVEELEAADADAVL
                     ADLRDTGAALAAILGGDVDERAAG"
     gene            1478..1870
                     /locus_tag="VFO47_10460"
     CDS             1478..1870
                     /locus_tag="VFO47_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HET8789158.1"
                     /translation="MANDDRRQPSRSPTTGRMPAEEAVTATQAVGKDPEMVELERRHS
                     RFVWPATAFFLIYYMALNVLAGTSPDLMGRKLFGEFTFGYLFALSQFVMAFVVAWVYA
                     RWAARRMDPLATDLREKLHDQQIKEAAK"
     gene            1867..>3399
                     /locus_tag="VFO47_10465"
     CDS             1867..>3399
                     /locus_tag="VFO47_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET8789159.1"
                     /translation="MSDQALSLTLFVVFVVITLGITIWASRQTKTAADFYSAGRRITG
                     WQNGVAISGDYMSAASFLGIAGLISLFGYDGFLYSVGWLVAYLTVLILIAELLRNSGK
                     YTMADVLAYRMRQRPVRSAAALSTVGVSIVYLIAQMVGAGALVKLLLGLEGDAAAILA
                     IAGVGVLMIVYVTFGGMIGTTWVQIVKAVLLMAGTVLLSFLVLTKFGFSMNKMFADAA
                     TASGKGDAFLAPGLRFTSTIELVSLGLALVLGTAGLPHILIRFYTVPTAQAARRSVIW
                     AIGIIGSFYILTTFLGFGAAALVGQERIKEADAAGNMAGPLLAQTVGGGEGSFGGDLF
                     LAFIAAVAFATILAVVAGLTITASSSFAHDFYANVMRRGQERDEAAEVRVARITALVI
                     GAIAIVLASFARGQNVAFLVGLAFAVAASANLPTILFSLYWKRFTTSGAVVGILVGLI
                     GSLLLVAVGPNVMGPKGLIFTDSEPLFPLENPGIVSIPLGFAAAWLGTMLSREPASEE
                     MYE"
BASE COUNT          453 a         1229 c         1198 g          519 t
ORIGIN      
        1 gagatcgacc tgggcaaggg ccgggtccgg cggctgctga agaccgtgcg cacgccgcgc
       61 ggcctctggc cgaccgccga cggccggcgg ctgtacgtgg ccgggttcga gaacggcgac
      121 ctccagcgga tcgacctggc cagcgggaag gccaagacgc tgctgcggac cggcggggcc
      181 atgcgccacc tggtcggcga cccgcagggc cgacgcctgt acgctgacga catggggacc
      241 aaccaggcgt tcgtggtcga cctggccaac gagcgggtgc gcaggctggc cggcaccgac
      301 aacgtgccca acaccatcga cctgaccccc gacgggcgag tcctgtacgt gtccaaccgc
      361 gggcgcaacg gggccagcta ctacaatccc gggccggagt gggggtcggt gctggccatc
      421 gacacggcca gcggcaaggt gctcgacgcc atggtcggcg gcaaccagac caccgggctg
      481 gacgtgtcgg ccgacggccg gctgctggcc ttctcggact tcctcgacaa ccgcatccac
      541 atctacgcgg tcccgccgta cgcggcgctg gccgccggcg gaggcggccg ggccgccgcc
      601 caccgggccg agctggccaa gcggtaaccc gggtgcgccg cctggtcctc tgggacatcg
      661 acggcaccct ggtccaggcc ggggaggtcg gccgcgacat cttcaccgag gccttccagg
      721 cggtcgtcgg gcgggcaccg gaccaggtcg ctgccaaggc gttggccatg gccgggcgga
      781 ccgacccgga gatcgcgctc gagttcctgg ccgcccacga gatcgccgag ggcgctacgc
      841 acctgccggc gttcagcgag gccctggtga cggccctggc ggccaaggcg gcggtgatcc
      901 gggagcgggg ccgggccctg cccggggccc gggagaccct ggccgcgctg gagcgcaccg
      961 acggcgtggt ccagtccctg ctgaccggca acgtgcagcc caacgccctg ctcaagctgg
     1021 ccagcttcga gctcgacggc tacctcgact tcgacgtcgg cgggttcggc tccgaccacc
     1081 accaccggcc cagcctggtc gaggtggccc gggccaaggc cgaggccaag tacgggacgg
     1141 ccttcgaggg cacggccacc gtgctggtcg gcgacacccc ccttgacgtc gccgccggcc
     1201 gcgccggcgg ggccagggtg gtcgcggtcg ccaccggccc ctaccgggtc gaggagctgg
     1261 aggcggccga cgccgacgcc gtcttggccg acctgcgcga caccggcgcc gccctggccg
     1321 ccatcctcgg cggcgacgtg gacgagcggg cggccggcta gcccagttct ccgccggagc
     1381 gacgcatatt tcgcatcgtc gccgggtagg tcgccagtgc tcgatttccg gtgctcacgc
     1441 gcccgacggc gggcgctccg gagcaaggag ggctcgcatg gccaacgacg accgtcgtca
     1501 accgtcgagg tcgcccacca ccggccggat gccggccgag gaggcggtga ccgccaccca
     1561 agccgtcggc aaggacccgg agatggtcga gctggagcgc cgccattcgc ggttcgtctg
     1621 gccggcgacc gccttcttcc tgatctacta catggccctc aacgtcctgg ccgggacctc
     1681 ccccgacctg atgggcagga agctgttcgg ggagttcacc ttcggctacc tgttcgccct
     1741 ctcgcagttc gtgatggcgt tcgtggtcgc ctgggtgtat gcgcgctggg ccgcccgccg
     1801 gatggacccg ctggccaccg acctgcgcga gaagctgcac gaccagcaga tcaaggaggc
     1861 ggccaagtga gcgaccaggc gctctccctg accctgttcg tggtcttcgt cgtcatcacc
     1921 ctcggcatca ccatctgggc gtcccggcag accaagaccg cggccgactt ctactcggcc
     1981 ggacgtcgca tcaccggctg gcagaacggc gtggccatct ccggcgacta catgtcggcg
     2041 gccagcttcc tcggcatcgc cgggctgatc tcgctgttcg gctacgacgg cttcctgtac
     2101 tcggtgggct ggctggtcgc ctacctcacc gtgctcatcc tgatcgccga gctgctgcgc
     2161 aactccggca agtacacgat ggccgacgtg ctcgcctacc ggatgcgcca gcggccggtg
     2221 cggtcggcgg ccgcgctgtc cacggtcggg gtctcgatcg tctacctgat cgcccagatg
     2281 gtcggggccg gcgccctggt caagctgttg ctcggcctcg agggggacgc ggccgccatc
     2341 ctggccatcg ccggggtcgg ggtgctgatg atcgtgtacg tcaccttcgg ggggatgatc
     2401 gggaccacct gggtccagat cgtcaaggcg gtcctgctga tggccgggac ggtgctgctg
     2461 tcgttcctgg tgctgaccaa gttcggcttc tcgatgaaca agatgttcgc cgacgcggcc
     2521 acggccagcg gcaagggcga cgccttcctg gcccccgggc tgcgcttcac ctcgaccatc
     2581 gagctggtct cgctggggct ggccctggtg ctgggcacgg ccgggctgcc ccacatcctg
     2641 atccggttct acaccgtgcc caccgcccag gcggcccgca ggagcgtcat ctgggccatc
     2701 ggcatcatcg gcagcttcta catcctgacc accttcctcg gcttcggcgc ggccgccctg
     2761 gtcggccagg agcgcatcaa ggaggcggac gcggccggca acatggccgg gccgctgctg
     2821 gcccagacgg tcggcggggg cgagggcagc ttcgggggcg acctgttcct ggccttcatc
     2881 gcggccgtgg ccttcgccac catcctggcc gtggtggccg ggctgaccat caccgcctcc
     2941 tccagcttcg cccacgactt ctacgccaac gtgatgcgcc gcggccagga gcgggacgag
     3001 gcggccgagg tccgggtggc gcggatcacc gccctggtca tcggggccat cgcgatcgtg
     3061 ctggcctcct tcgcccgcgg gcagaacgtc gccttcctgg ttgggctggc cttcgccgtg
     3121 gccgccagcg ccaacctgcc gacgatcctg ttctcgctct actggaagcg gttcaccacc
     3181 agcggggcgg tggtcggcat cctggtcggg ctgatcggct cgctgctgct ggtggccgtc
     3241 ggtcccaacg tgatgggccc caagggcctg atcttcacgg actccgagcc gctgttcccg
     3301 ctggagaatc cgggcatcgt ctcgatcccg ctcggcttcg cggccgcctg gctcggcacc
     3361 atgctgtcca gggagccggc ctcggaggag atgtacgag
//
LOCUS       DASSWR010000626         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_899809,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000626 DASSWR010000000
VERSION     DASSWR010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899809"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="pcaH"
                     /locus_tag="VFO47_10470"
     CDS             <1..663
                     /gene="pcaH"
                     /locus_tag="VFO47_10470"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET8789160.1"
                     /translation="DFPGYRSTRLRYPHQPLVPLPHRLTEVTGPLLGEGRVGETDHDL
                     TVQHAGEPLGERITVTGRVLDGDGRPVPGTLVEVWQANAGGRYRHDGDRHPAPLDPNF
                     SGAGRCLTDAEGRFRFVTVKPGAYPWTNHPNAWRPAHIHFSLFGRAFVQRLITQMYFP
                     GDPLFAYDPILQSVPEAARERLVSRLDMDLTTPEWALGYRWDIVLRGRQATPLEDEDD
                     DR"
     gene            660..1220
                     /gene="pcaG"
                     /locus_tag="VFO47_10475"
     CDS             660..1220
                     /gene="pcaG"
                     /locus_tag="VFO47_10475"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET8789161.1"
                     /translation="MSLPETPSQTIGPFFAVGLPWPDGPDVVPAGTTGAVQIGGQVVD
                     GAGDPVPDALVETWQADPDGRFAHPDDPSGPATSDFRGFGRCATDADGRWAVRTLKPG
                     PLPVPGGGVEAPHLNLSVFARGLLNRLVTRVYFPDEADANAADPLLASIADPAVRARL
                     VAVADGDGLRFDIHLQGARETPFLAI"
     gene            1275..1760
                     /locus_tag="VFO47_10480"
     CDS             1275..1760
                     /locus_tag="VFO47_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789162.1"
                     /translation="MTEIDVRADQMARCVTCGSELHPERAERYSYCPKRECREQNARP
                     LTIAAVAVSKAADEYLVLDERTTQDLAAGRYQDQRKSSAVTDGPRPERARVPAAGSRP
                     THRAPARQRPRRSWTKAQEELALIYSARGMRPDEIAERLGLSRHTVTQLLLSAKDRGR
                     R"
     gene            complement(1817..>2633)
                     /locus_tag="VFO47_10485"
     CDS             complement(1817..>2633)
                     /locus_tag="VFO47_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8789163.1"
                     /translation="DDSVDGVIVASPTPTHVDLLTGAVKAGKAVLCEKPIDFDIARVD
                     ACWAEIASLSPVVMIGFNRRFDRSFREVRDRVRAGDIGRLEQVAITSRDPAPPPREYI
                     PASGGLFRDMTIHDFDMARFLLGDVAEVQAMGANLIEPFIEEAGDIDSAVVVLRSADG
                     ALAHITNSRRCSFGYDQRVEAFGSTGMLTAHNQLPTSVRHASADHSEAANPYLTFIRE
                     RYGASYPAELDHFITSVETGTQPDPTFADGREALVLADAANESLRTGGTVRVS"
BASE COUNT          362 a          981 c          959 g          331 t
ORIGIN      
        1 gacttccccg gctaccgctc gacccggctg cgctaccccc accagccgct ggtgccgctg
       61 ccgcaccggc tcaccgaggt caccgggccg ctgctgggcg aagggcgggt cggcgagacc
      121 gaccacgacc tgacggtcca gcacgccggg gagcccctgg gcgagcggat caccgtcacc
      181 gggcgggtcc tggacggcga cggccgcccg gtccccggca ccctggtcga ggtctggcag
      241 gccaatgccg gcggccgcta ccgccacgac ggcgaccgcc accccgcccc cctggacccc
      301 aacttcagcg gcgccggccg ctgtctgacc gacgccgagg gccgcttccg gttcgtgacc
      361 gtcaagccgg gcgcctaccc gtggaccaac caccccaacg cctggcggcc cgcccacatc
      421 cacttctcgc tgttcgggcg ggcctttgtc cagcggctga tcacccagat gtacttcccc
      481 ggcgacccgc tgttcgccta cgacccgatc ctgcagtcgg tgcccgaggc ggcccgggag
      541 cggctggtct cccggctcga catggacctg acgaccccgg aatgggcgct cgggtaccgc
      601 tgggacatcg tgctccgcgg ccgccaggcc actcccctgg aggacgagga cgacgaccga
      661 tgagcctgcc cgagaccccc tcccagacga tcgggccgtt cttcgccgtt gggctgccct
      721 ggcccgacgg gcccgacgtg gtccccgccg gcacgaccgg ggcggtccag atcggcggcc
      781 aggtggtcga cggcgccggc gaccccgtac ccgacgcgct ggtcgagacc tggcaggccg
      841 acccggacgg ccgcttcgcc caccccgacg acccctccgg gccggccacc tccgacttcc
      901 gggggttcgg ccgctgcgcc accgacgccg acggccgctg ggcggtccgg accctcaagc
      961 cgggaccgct cccggtcccc gggggcgggg tcgaagcccc gcacctgaac ctgtcggtgt
     1021 tcgcccgcgg cctgctcaac cgcctggtca cccgggtcta cttccccgac gaggccgacg
     1081 ccaacgccgc cgaccccctc ctcgcctcga tcgccgaccc ggccgtgcgg gcgaggctgg
     1141 tcgcggtcgc cgacggcgac gggctgcgct tcgacatcca cctccagggc gcccgggaga
     1201 cccccttcct cgccatctga cccggagcca ggacatggtg gaacacctgc cagcgacaac
     1261 gcgttgaacc gccgatgact gagatcgatg ttcgcgccga ccagatggct cggtgcgtca
     1321 cgtgcgggag cgagctgcat cccgaacggg cggagcggta cagctactgc ccgaagcggg
     1381 agtgccggga gcagaacgcc cggccgctga cgatcgcggc cgtggccgtg agcaaggccg
     1441 ccgacgagta cctcgtgctg gacgagcgga ccacccagga cctggccgcc ggccgctacc
     1501 aggaccagcg gaagtcctcg gcggtcaccg acgggcccag gcccgagcga gcccgggtgc
     1561 cggcggccgg ctccagaccc acccaccggg cgccggcccg gcaacgacca cggcgctcct
     1621 ggaccaaggc ccaggaagag ctggcgctga tctacagcgc cagggggatg cggccggacg
     1681 agatcgccga gcggctcggc ctgagccgcc acaccgtcac ccagctgctc ctgtcggcca
     1741 aggaccgcgg gcggcgataa ctgccccgga ccgggacacc cgaccggcgg tcgagcggag
     1801 gggtgctgcg ccgcggctag gacacccgga cggtgccgcc ggtgcggagg ctctcgttgg
     1861 cggcgtcggc caggaccagg gcctcgcggc cgtcggcgaa ggtggggtcg ggctgggtgc
     1921 cggtctcgac cgaggtgatg aagtggtcga gctcggcggg gtaggaggcg ccgtagcgct
     1981 cgcggatgaa ggtcaggtag gggttcgcgg cctcgctgtg gtcggcggac gcgtggcgga
     2041 cgctggtcgg gagctggttg tgggcggtga gcatcccggt gctgccgaag gcctccacgc
     2101 gctggtcgta gccgaagctg cagcggcggc tgttggtgat gtgggccagg gcgccgtcgg
     2161 ccgagcggag gacgacgacg gcgctgtcga tgtcgccggc ctcctcgatg aagggctcga
     2221 tcaggttggc tcccatggcc tgcacctcgg ccacgtcgcc gagcaggaag cgggccatgt
     2281 cgaagtcgtg gatggtcatg tcgcggaaca ggcccccgga ggccgggatg tactcccggg
     2341 gcggcggcgc cgggtcccgg ctggtgatcg cgacctgctc caggcggccg atgtcgccgg
     2401 cccggacgcg gtcccggacc tcccggaacg agcggtcgaa gcggcggttg aagccgatca
     2461 tcaccaccgg cgacagcgag gcgatctcgg cccagcaggc gtcgacccgg gcgatgtcga
     2521 agtcgatcgg cttctcgcac agcaccgcct tgcccgcctt gacggcgccg gtcagcaggt
     2581 cgacgtgggt cggggtcggg gaggcgacga tcacgccgtc gacgctgtcg tcg
//
LOCUS       DASSWR010000627         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_221481,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000627 DASSWR010000000
VERSION     DASSWR010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221481"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..2010
                     /locus_tag="VFO47_10490"
     CDS             511..2010
                     /locus_tag="VFO47_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET8789164.1"
                     /translation="MSMSQVGSGLPRLLAERYELSEQVAAGGMTSVWRGHDRVLGRDV
                     VVKVLHPELAADPSFRARFHEEAVNAARLTHPNIVALYDTGELGDVAYIVMELVNGPT
                     LREALGRHGPLPPSRAARLSMEVAAALDYAHQAGVCHGSLKPGNILLADDGTVKVADF
                     SIARAATEEDPGRTGEVLGADGHLAPEVVNGDDADGRADVFGLGACLYEMLTGRPPGA
                     DPSTTIPPRALRAGVSRDLDTIVRQAMAPDPADRFQTVQAMAAALSRSAAGADSVPPS
                     DFLPVPQAPTGPEVTPARGFLRHEGRLLGWVLALVALAAVLVAVGLTLAKDDLSGLFG
                     EDPPARPRGSQTTTAATTPIRGVTPAAYDPLGDGGEHDNEVRNAIDRNPATTWETDRY
                     KSAALGDLKAGVGFVLSLDTPRKARQLQLDLTAAGSDFTVYSTADDVLPGAIDGGGWR
                     PVSSRKDAADQLTVKLSGEPHRHYLVWFTKLPPDGSGYRIGIAEAVLRS"
     gene            2007..2735
                     /locus_tag="VFO47_10495"
     CDS             2007..2735
                     /locus_tag="VFO47_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8789165.1"
                     /translation="MSDPSDEALLAAHLRGDPRAFGELVARHERRIYGLCLRILGNRE
                     DAEDATQEAFLAALRKAASFRKAAAFSTWLYRIAVNAATDQARRRGRARLATLDPEAA
                     GLAVAPGSELGEVVASAVAVQTALTQVPEEFRVAVVLCDLYRVPYSDAAQILEVPVGT
                     VKSRVFRGRLALAECLGAPPDTDPGHPPLPVPGPQGTGTAPPASEPTGPDSTRSSSSV
                     PNLHDHQRQGQPRLPDSHDHERQG"
BASE COUNT          383 a         1159 c          936 g          333 t
ORIGIN      
        1 gctccgggtc gaggagatca gcctcctgct cgacatcgtc cgccgccggg gccggagggc
       61 ccggggggtc cgggggacac ggtccaacgc ttcctgacga atcttcccgg cagtcccgga
      121 cgacctcggg cggggccgca acggaatggc gccgccatct gtctgcggcg gacacgccgt
      181 gagggtcccc gcccgccgcc tcgaccctcg gccagctcct cgccgccgac agcgtccacc
      241 aggcgctggt gaccaccaag taccaggagc aggtggtcgg ggggccgacc gcgccgccct
      301 cgaccgggtc gatcgcctgc gccgggaggt cgaggcgctc gccgccaggc tggctggagc
      361 gcgtccggcc ccgcccccat ccaccatgtc ggcatgtaca tcggcgccgt ccggccgacc
      421 gggtgaccgt tccgagcggt cggaaaggat ccttcttgtt agacttcggg ggtcgcgatc
      481 cccggggtcc aacgccccgc tcttcgtaat atgagcatgt cgcaggtcgg ctccggtctg
      541 ccccgactgc tcgccgagcg gtacgagctc agcgagcagg tcgcagcggg cggcatgaca
      601 agcgtctggc gcgggcacga ccgtgtcctc ggacgggacg tggtcgtcaa ggtgctccac
      661 cccgagctgg ccgccgaccc cagcttccgg gcccgcttcc acgaggaggc ggtgaacgcc
      721 gcccgcctga cccaccccaa catcgtcgcc ctctacgaca cgggcgagct gggcgacgtc
      781 gcctacatcg tgatggagct ggtcaacggg ccgaccctgc gcgaggcgct cggccgccac
      841 ggcccgctgc cgccgtcgcg ggcggccagg ctctcgatgg aggtcgccgc cgccctcgac
      901 tacgcccacc aggccggggt ctgccacggc agcctcaagc cgggcaacat cctgctcgcc
      961 gacgacggca ccgtgaaggt ggccgacttc tcgatcgcgc gggcagcgac cgaggaggac
     1021 cccggccgca ccggggaggt cctcggggcc gacggccacc tggcccccga ggtggtgaac
     1081 ggcgacgacg ccgacggccg ggccgacgtg ttcgggctcg gggcctgcct ctacgagatg
     1141 ctgaccggcc ggcctcccgg tgccgacccc tccaccacga tcccgcccag ggccctgcgg
     1201 gccggcgtct cgcgcgacct ggacacgatc gtccgccagg cgatggcccc cgaccccgcc
     1261 gaccgcttcc agaccgtcca ggcgatggcc gcggccctgt cccggtcggc cgcgggcgcc
     1321 gacagcgtcc cgccgagcga cttcctcccg gtcccccagg ccccgaccgg ccccgaggtc
     1381 acccccgccc gcggcttcct gcgccacgag ggccggctcc tcggctgggt cctcgccctg
     1441 gtcgccctgg ccgccgtcct ggtcgcggtc ggcctcaccc tggccaagga cgacctcagc
     1501 ggcctgttcg gcgaggatcc gcccgcgaga ccgcgcggga gccagacgac caccgccgcg
     1561 accaccccca tccggggcgt gaccccggcc gcctacgacc ccctgggcga cggcggcgag
     1621 cacgacaacg aggtacgcaa cgcgatcgac cggaacccgg ccaccacctg ggagaccgac
     1681 cgctacaagt ccgccgccct gggcgacctc aaggccgggg tggggttcgt gctcagcctg
     1741 gacacgccca ggaaggcccg ccagctccag cttgacctga ccgcggccgg ctccgacttc
     1801 accgtctact ccaccgccga cgacgtcctc cccggcgcca tcgacggcgg cggctggcgg
     1861 cctgtcagca gccgcaagga cgcggccgac cagctcacgg tgaagctctc cggcgagccg
     1921 caccggcact acctcgtctg gttcaccaag ctgccgccgg atgggtccgg gtaccgcatc
     1981 gggatcgccg aagccgtcct tcgatcgtga gcgacccgag cgacgaggcc ctgctcgccg
     2041 cccacctgcg aggcgacccc cgggccttcg gcgagctggt cgcgcgccac gagcggcgca
     2101 tctacgggct ctgcctgcgg atcctcggca accgcgagga cgccgaggac gccacccagg
     2161 aggccttcct ggccgccctg cgcaaggcgg ccagcttccg caaggcggcc gccttctcga
     2221 cctggctcta ccggatcgcc gtgaacgccg ccaccgacca ggcccgccgc cggggccggg
     2281 cccggctggc caccctcgac ccggaggccg cgggcctggc cgtcgccccg gggagcgagc
     2341 tcggcgaggt ggtcgcctcc gccgtggccg tccagaccgc cctcacccag gtccccgagg
     2401 agttccgggt cgcggtcgtc ctctgcgacc tgtaccgggt tccctactcc gacgccgccc
     2461 agatcctcga ggtcccggta ggcaccgtga agagccgcgt gttccgcggc cgcctcgccc
     2521 tcgccgaatg cctcggcgcc ccaccggaca ccgatcctgg ccacccacca ctccccgtac
     2581 ccggccccca gggaaccggg accgccccgc cagcgtccga acctacaggg cccgactcga
     2641 ccaggagcag ctccagcgtg cccaacctcc acgaccacca gcggcagggc cagccccgcc
     2701 tgcccgactc ccacgaccac gagcggcagg ggtagccgac cgtgcccgac cgtcccgacc
     2761 accccgaccg cgagcagctc gccgccttcc aggccggcga cggcgaacgc c
//
LOCUS       DASSWR010000628         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_391380,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000628 DASSWR010000000
VERSION     DASSWR010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391380"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFO47_10500"
     CDS             <1..789
                     /locus_tag="VFO47_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789166.1"
                     /translation="TAPEPGANQRKLGWTARAGGLTVTAVEASRVATEQRETVASISF
                     RVQGVPAGQRVLALRGLRLLDSGGGVYASVEQRQFSSEGGVPVRPVDGDPGLYRVVTG
                     PAPRLSSLARIDLCGGVAVRARDRTIALDTSGAWPAGPQMRTIDPGQDDAVRPDLDSA
                     RVLGTDLQLRVTSAFVRDGRAVVVVDASSGFRGVPGQYLPLGAELRAGDRVLCTRTSL
                     LGEDMGQQLVQGIVLSCPTRPVPRLTVAVGVGARIIPFDATLRP"
     gene            939..>2256
                     /locus_tag="VFO47_10505"
     CDS             939..>2256
                     /locus_tag="VFO47_10505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET8789167.1"
                     /translation="MDPDERERIGFDPSPEAPIPVVVELNLRHRNGLQGAADQFIAQY
                     RAALPGAPEPDRVADTYYRCELTEAALLDLVEEDQRQEDPARRAIYRVWPDFPVRPLI
                     DLSCATVKADAARRSFDATGRGITWAVLDSGIDEHHHHFRRYNTLKGEVAGLHRDFTM
                     SGEPALGGALVDKYGHGTHVAGIIAGGLPASMPRGSDLKVVERVFDPARPETPKVQDR
                     EVKPPEMLAGIARATKLVSLKVLDERGDGRSMNVVRALEYVRKDINGYGKLLRVHGVN
                     LSLGYEFDPKWFACGQSPICAEVDRLVRSGVVVVVAAGNTGYGSLTALARHTNVGLTM
                     TINDPGNAALAITVGATHRDMPHTYGVSYFSSKGPTGDGRLKPELVAPGERITSCAAG
                     KALASAFPGRQPPSPEKTAVYVEDSGTSMAAPHVSGAVANFLSIRRE"
BASE COUNT          299 a          908 c          783 g          266 t
ORIGIN      
        1 accgcccccg agccgggcgc caaccagcgc aagctgggct ggacggcccg ggccggcggc
       61 ctgaccgtga ccgccgtcga ggccagccgg gtggccaccg agcagcgcga gacggtcgcc
      121 tcgatcagct tccgcgtcca gggcgtgccc gccggccagc gggtcctcgc cctgcggggg
      181 ctgcggctgc tcgactccgg cggcggggtg tacgccagcg tggagcagcg gcagttcagc
      241 agcgagggcg gcgtcccggt ccggcccgtc gacggcgacc ccggcctcta ccgggtcgtc
      301 accgggcccg ccccccggct gtcgtcgctg gcccggatcg acctgtgcgg cggggtggcc
      361 gtccgggccc gcgaccggac gatcgccctg gacacctccg gggcctggcc ggccggcccg
      421 cagatgcgga ccatcgaccc cggccaggac gacgccgtcc ggccggacct cgactcggcc
      481 cgggtcctcg ggaccgacct ccagctccgg gtgacctcgg cgttcgtgcg cgacgggcgg
      541 gcggtggtgg tggtcgacgc ctcctcgggc ttccggggcg tccccggcca gtacctgccc
      601 ctcggcgccg agctgcgcgc cggcgaccgg gtcctgtgca cgcgcacgtc gctgctgggc
      661 gaggacatgg gccagcagct cgtccagggg atcgtgctca gctgcccgac ccggccggtg
      721 ccgcggctca ccgtcgccgt cggggtcggt gccagaatca tccccttcga cgccaccctg
      781 cggccctgac catcgctgga ggacccatgc ccgacgagcc cacagatgcg gggggcggag
      841 ccccccgcac accccccgaa cccgaaccgc cggcgccgca gccgccgcgc cggcgggacc
      901 agccgctggt ccagcgggcg attgtctccg acgccctgat ggaccccgac gagcgggagc
      961 ggatcggctt cgaccccagc cccgaggccc ccatcccggt ggtggtcgag ctcaacctgc
     1021 gccaccgcaa cgggctccag ggcgccgccg accagttcat cgcccagtac cgggccgccc
     1081 tccccggcgc ccccgagccc gaccgggtgg ccgacaccta ctaccgctgc gagctgaccg
     1141 aggcggcgct gctggacctg gtcgaggagg accagcgcca ggaggacccc gcgcggcggg
     1201 ccatctaccg ggtctggccc gacttcccgg tccgccccct gatcgacctc tcctgcgcca
     1261 ccgtcaaggc ggacgcggcc cggcgctcct tcgacgccac cggccgcggc atcacctggg
     1321 cggtgctgga ctccggcatc gacgagcacc accaccactt ccgccgctac aacaccctca
     1381 agggggaggt cgccggcctc caccgcgact tcaccatgag cggcgagccg gccctcggcg
     1441 gcgccctggt cgacaagtac ggccacggca cccacgtcgc cggcatcatc gccggcggcc
     1501 tgccggccag catgccgcgg ggcagcgacc tcaaggtggt cgagcgggtc ttcgacccgg
     1561 cccgccccga gacccccaag gtccaggacc gcgaggtcaa gccgcccgag atgctggccg
     1621 ggatcgcccg ggcgaccaag ctggtcagcc tcaaggtcct ggacgagcgg ggcgacggcc
     1681 ggtccatgaa cgtggtccgg gcgctggagt acgtgcgcaa ggacatcaac ggctacggca
     1741 agctgctgcg ggtccacggg gtcaacctca gcctcggcta cgagttcgac cccaagtggt
     1801 tcgcctgcgg ccagagcccc atctgcgccg aggtcgaccg cctcgtccgc tccggggtgg
     1861 tggtggtggt ggccgccggc aacaccggct acggcagcct caccgccctg gcccgccaca
     1921 ccaacgtcgg gctgaccatg acgatcaacg accccggcaa cgccgccctg gccatcaccg
     1981 tcggcgccac ccaccgggac atgccccaca cctacggggt gtcgtacttc tcctccaagg
     2041 gccccaccgg cgacggccgg ctcaagccgg agctggtcgc ccccggcgag cgcatcacct
     2101 cgtgcgccgc cggcaaggcg ctggccagtg ccttccccgg ccggcagccg ccctcgcccg
     2161 agaagacggc ggtgtacgtc gaggactccg ggaccagcat ggccgccccc cacgtgtcgg
     2221 gggcggtggc caacttcctg tccatccggc gggagt
//
LOCUS       DASSWR010000629         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_221553,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000629 DASSWR010000000
VERSION     DASSWR010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221553"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..673)
                     /locus_tag="VFO47_10510"
     CDS             complement(185..673)
                     /locus_tag="VFO47_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789168.1"
                     /translation="MTEDGRFIALARFESEQAARRNSDRPEQDRWWAETSKLFTGEAS
                     FKDSSDVTVDVTGDPDAAGFVQVMQGRGSDPERARELMSQDSAAWAAFRPDVVGSVAV
                     GHEGGAYTMAMYFTSEAEAREGERKEPPPELKAQMEEMQQLSIGEPEFFDLKQPWLYA
                     PR"
     gene            1441..2649
                     /locus_tag="VFO47_10515"
     CDS             1441..2649
                     /locus_tag="VFO47_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HET8789169.1"
                     /translation="MTPAELARVRRRLVAFAEDIFQPLGRVDQRRWGEVYLRGLMLDG
                     KRKSIQPMAERLPDGNEQALQQFVSQSPWDWRPVRQRLATQMTAVVDPDAWIIDDTGF
                     PKFGNASVGVARQYSGTLGKVANCQVGVSIHAATDQASCPIDWRLFLPASWDADGERR
                     RAAHVPEAVGHRPKWQLALDMVDELAGWGLVPPVVVADAGYGESGELRLGLEARGLAY
                     VVQVKATTSAYPEAIRLEPAPYAGRGRRPRARYRAKRSSLRALTLVAGPGTAKTVAWR
                     EGVGGKLRSRFVVLGVRPAGVKLRRAAAGGELPVRWLLAEWPSDQPEPVKYWLANLPD
                     DPALVELVRLAKLRWRIEHDYRELKDGLGLDHFEGRSFQGWHHHVTLVSTAHAFVTQQ
                     RLDPKPAAPA"
     gene            complement(2790..3326)
                     /locus_tag="VFO47_10520"
     CDS             complement(2790..3326)
                     /locus_tag="VFO47_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8789170.1"
                     /translation="MIPRSFPPAPGWTPDGHRVKALLDYGRGPEKTWVYGALRVGDGQ
                     EVTMTAPSRNSIGYQQLLAAVEMANPAGTIMVITDNLSSHTSASTRAWLADHPRIQHA
                     FIPKGACWLNLQEGWWRLFRREAFAGQSFATPDEIALATRVATCQLNAYARPWVWGRP
                     PPSPRHRRPTFTYRISGT"
     gene            complement(3350..>3465)
                     /locus_tag="VFO47_10525"
     CDS             complement(3350..>3465)
                     /locus_tag="VFO47_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8789171.1"
                     /translation="RVRWRRTRSWATSTDPDFAPKEPASSPSTPPRPRRRR"
BASE COUNT          515 a         1186 c         1191 g          573 t
ORIGIN      
        1 tcctcgaagc ggtcacgctc aactgtctcg gtcagaacac ctccgtgcga gtaagtggca
       61 gcccaccgct gtgcagaccc gcgtttccgc aggtcgccgc ggaccgtcag gggcgaagtt
      121 aggcgttctc ctgccgccgc ccaagcccaa caagagatgc cagccccagg ccggtgagcc
      181 cccctcagcg tggggcgtac agccagggtt gcttgaggtc gaagaactcc ggctcgccga
      241 tgctcagctg ctgcatctcc tccatctggg ccttcagctc cggtggcggc tccttgcgct
      301 cgccttcgcg ggcctccgcc tcagaggtga aatacatcgc catggtgtag gccccaccct
      361 catgacccac cgccacactg ccgacgacat cgggacggaa ggcagcccac gccgccgagt
      421 cctgactcat gagctcccgc gcccgctccg ggtcgctgcc ccggccctgc atgacctgga
      481 caaagccggc cgcatccggg tcgccggtca cgtccacggt gacgtcgctg ctgtccttga
      541 agctggcctc cccggtgaac aacttggagg tctcggccca ccatcggtcc tgttcgggtc
      601 ggtcgctgtt acgccgggca gcctgctcgg actcaaagcg ggccagggcg atgaagcggc
      661 catcctcggt cacccccgcg gtcgagccca gccagccggt ggccccgggg gcgagctcct
      721 gggcccaccg gtcgagcgct tcccggacct tcccggcgtc ggtcacctgt ccctgaatca
      781 cctgaacaaa caccacgacc ccctctgctg ggcggaccac tccgctatgg cctcgagtgc
      841 gcctcagacc ctacgacctc ccggtgccga ggtcgagacc gccaacgacc aaggacggtc
      901 cggtccccgg cgtgggttcc tgcagctcta aggcattgac ctcaggcagc tggttaggag
      961 atacctgcca atcttctgtt ctctacggtt cagttgggtc cagccaggtc aggttagtcg
     1021 gcgattctgg tgagtccggt ctggttgggt gcagttgtgg cctgtggaat gaccgcgaga
     1081 atgaccacca gagcaagcaa tgaggctcgg tccggtgcgt ctgagcctcg cccaaggccc
     1141 ggtccctcat cccgaccgcc gagccgaggt tgcgctgcgc caagtgcagg ctgggcagct
     1201 aggcgcagcg gtcgtccgcg acaacgtctg gcttgcaacc gaccgcctcc cgaccctagg
     1261 actgcaggtt gcggcagttg cagagatgag gagcgattct gcgacttgtc atgtcatcga
     1321 atcggtgtca gcgacctagt agtgctccgt taagtttgct ctggggtggt ctgctacggg
     1381 tgaatctcct cgacaatgtt gtcgcatcgt cgaggagggc agcgtgctgg tcaggacccg
     1441 atgaccccgg ccgagctggc ccgggtgcgc aggcgcctgg tcgccttcgc cgaggacatc
     1501 ttccagccct tgggccgcgt cgatcagcgc cgctggggtg aggtctatct gcgtgggttg
     1561 atgctggacg gcaagcgcaa gtcgatccag cccatggccg agcgcctgcc ggacggcaac
     1621 gagcaggcgc tgcaacagtt cgtcagccag agcccctggg actggcggcc ggtccgccag
     1681 cggctcgcca cccagatgac ggcggtggtc gacccggacg cctggatcat cgacgacacc
     1741 ggcttcccca agttcggcaa cgcctcggtc ggggtcgccc gccagtactc gggcacgctc
     1801 ggcaaggtgg ccaactgcca ggtcggggtg tccatccacg cggccactga ccaggcgagc
     1861 tgcccgatcg actggcggct gttcctgccc gcgagctggg acgccgatgg ggagcggcga
     1921 cgcgccgccc atgtgcccga ggcggtgggg catcgaccca agtggcagct ggccctggac
     1981 atggtggatg agcttgccgg ctgggggctt gtgccgccgg tggtggtcgc cgatgccggc
     2041 tatggggagt cgggcgagct gcgcctgggg ctggaggcgc gcgggctggc ctatgtggtg
     2101 caggtcaagg ccaccaccag cgcctatccg gaggcgatcc gcctcgaacc cgcgccctat
     2161 gcgggtcggg gccgccgacc ccgagcacgc taccgggcca agcggtccag cctgcgagcg
     2221 ctgaccctgg tcgccgggcc aggcacggcc aagaccgtcg cctggcgtga gggtgtcggt
     2281 ggcaagctgc gctcgcggtt tgtggtcctt ggggtccgcc cggccggggt gaagctgcgt
     2341 cgcgccgccg ccggtgggga gctgccagtg cgctggctgc tggccgagtg gcccagcgac
     2401 cagcccgagc cggtcaagta ctggctagcg aacctgcccg acgaccctgc cctggtggag
     2461 ctggtccggc tggccaagct ccgctggcgc atcgagcatg actaccgcga gctcaaagac
     2521 ggccttggcc tcgaccactt cgagggccgc agctttcaag gctggcacca tcacgtcacc
     2581 ctggtctcga ccgcccacgc cttcgtcacc cagcagcggc tggacccaaa accagccgcg
     2641 ccggcctgac cacctaccag gtattgcgtg agctgcaaac cctgctcgcc tgttgggccg
     2701 gcgccggccc ctctgccagc gccccctgcc acgctccacc ccctggctgc acccatcagc
     2761 ccacccgacc taacggagca ctactagtgc tacgttcctg agatccggta ggtaaaggtg
     2821 gggcgtcggt ggcgtgggga tggtggtgga cgaccccaca cccatggacg ggcgtaggcg
     2881 ttgagctggc aggtcgccac cctggtggcc agggcgattt cgtcgggggt ggcaaacgac
     2941 tggccggcga aggcttcgcg gcgaaacagc cgccaccagc cctcctggag gttgagccag
     3001 caggcccctt tggggatgaa ggcatgctgg atgcgggggt ggtcggccag ccaagcacgg
     3061 gtcgaggcgc tggtgtggct ggagaggttg tcggtgatca ccatgatggt cccggccggg
     3121 ttggccatct cgacggcggc cagcagctgc tggtagccga tcgagttgcg ggagggtgcg
     3181 gtcatcgtca cctcctgccc gtcgccgacc cgcagcgcgc cgtacaccca ggtcttctct
     3241 ggcccgcggc cgtagtccaa cagcgccttg acccggtggc cgtcaggggt ccagccgggc
     3301 gcgggcggga agctgcgagg gatcaccggg ccgagttcgt cggcgcagat caccgtcgtc
     3361 gcctcgggcg gggtggtgta gagggcgatg acgcgggctc ttttggggcg aagtccgggt
     3421 cggtgctggt cgcccacgag cgggtccgcc gccagcgcac ccgtt
//
LOCUS       DASSWR010000630         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1182988, whole genome shotgun sequence.
ACCESSION   DASSWR010000630 DASSWR010000000
VERSION     DASSWR010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182988"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFO47_10530"
     CDS             <1..274
                     /locus_tag="VFO47_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET8789172.1"
                     /translation="EVVITGVGLADAQRVTFGGVEATNLRQVSASELRVTVPDGAVSG
                     LVTVHTPAGIADSPRSFTVGEPAEAPPAKAEQDQENQEEGRPQATR"
     gene            complement(298..1227)
                     /locus_tag="VFO47_10535"
     CDS             complement(298..1227)
                     /locus_tag="VFO47_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HET8789173.1"
                     /translation="MHVFFLHAPRDLGHPDLRHSHARIGHAVTRDLHSWEVLDTALSP
                     GPPGAFDDQATWTGSVLEAGGRWHMFYSGISTRDEGRVQRVGLATSTDLVTWERQPLV
                     LEADPRWYETSRDHPRGEETWRDPWVFADPGGDRFHMVVCARVNHGPPDGRGVLGHAW
                     SADLRSWEVGPPLSAPGEFSQLEVPQLVRVGGAWRAVFSAWWSDHSATRLARPGVVAE
                     GGTHYLVSPGPLGPYVLDRDAFLLGDPLISFYAGRVLHHRDAWWLFAWRHVDGHGRFF
                     GELSDPMPLTVDEAGSLWVRDSPLDGRQAEAAP"
     gene            complement(1316..2251)
                     /locus_tag="VFO47_10540"
     CDS             complement(1316..2251)
                     /locus_tag="VFO47_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8789174.1"
                     /translation="MTTTGTTPPPTPVPGEVKPRRAIPRREGGAAPGAGQANWVVRLA
                     LVILCFLWIVPTLGIVITAFRTADAANTSGWWTVLTSPLDFTQYTLANYSQAWNEGMG
                     NAFINSFAVTLPATVIPIMIAAFAAYAFTFMQFPFRDVLFVVIVALLVVPNQVALVPL
                     LRFYGDVGLTGQFAAVYLAHIGFGMPLAVYILRNYMSTLPKAVIESAKIDGASHFKTF
                     WRLIVPMSVPALASFAIFQFLWVWNDLLIALLFLGPGDREVVTVTVAGLVGAQNQGWQ
                     LLTAGAIISMIVPVLVFISLQRFFVRGLTAGSVKG"
BASE COUNT          367 a          779 c          847 g          269 t
ORIGIN      
        1 cgaggtcgtc atcaccgggg tcggcctggc cgacgcccag cgggtcacct tcggaggggt
       61 cgaggccacc aacctgcgcc aggtctcggc gagcgagctg cgggtgaccg tccccgacgg
      121 cgcggtcagc ggcctggtca ccgtccacac ccccgccggg atagccgaca gcccgcggtc
      181 cttcacggtc ggcgagcccg cggaggcacc ccccgccaag gccgagcagg accaggagaa
      241 ccaggaggag gggcggccac aagccaccag gtgaccggga tgcgcaggat cgccgcgcta
      301 gggcgccgcc tcggcctgcc ggccgtccag ggggctgtcc cggacccaca gcgacccggc
      361 ctcgtcgacg gtcaggggca tcgggtcgct gagctcgccg aagaaccggc catggccgtc
      421 gacgtggcgc caggcgaaca gccaccatgc gtcgcggtgg tgcagcaccc ggccggcgta
      481 gaagctgatc agggggtcgc cgaggaggaa ggcgtcgcgg tccaggacgt aggggccgag
      541 ggggccgggc gagaccaggt agtgggtgcc gccctcggcg accaccccgg ggcgggccag
      601 gcgggtggcg ctgtggtcgc tccaccaggc cgagaacacg gcccgccagg cgccgccgac
      661 ccgcaccagc tggggcacct ccagctggct gaactcaccc ggggccgaca gcggtgggcc
      721 gacctcccag gagcgcaggt cggccgacca ggcgtggccg aggacgccgc gcccgtcggg
      781 cgggccgtgg ttgacccggg cgcagacgac catgtggaac cggtcgccgc ccgggtcggc
      841 gaacacccac gggtcccgcc aggtctcctc gccccgcggg tggtcgcggc tggtctcgta
      901 ccagcgcggg tcggcctcca gcaccagcgg ctggcgctcc caggtgacca ggtcggtgga
      961 ggtggccagc ccgacccgct gcacgcggcc ctcgtcccgg gtggagatgc ccgagtagaa
     1021 catgtgccag cgcccaccgg cctccaggac actcccggtc caggtggcct ggtcgtcgaa
     1081 ggcccccggc ggccccgggc tgagggcggt gtcgagcacc tcccagctgt ggaggtcccg
     1141 cgtcacagca tggccgatcc gggcgtggct gtggcgcagg tccgggtgcc cgaggtcgcg
     1201 gggagcgtgc aggaagaaga cgtgcacgtc cccgccgtcg acggcgaacc agaagtccca
     1261 gatccagtgt ccgggaagcc gcaggctcag cgccggccct cgctcaccgc cctttctatc
     1321 ccttgaccga cccggcggtg aggccgcgga cgaagaagcg ctggagcgag atgaagacca
     1381 gcaccgggac gatcatgctg atgatggccc cggccgtcag cagctgccag ccctggttct
     1441 gcgccccgac cagcccggcc acggtcacgg tgacgacctc gcggtcgccc gggccgagga
     1501 acagcagggc gatcagcagg tcgttccaga cccacaggaa ctggaagatg gcgaacgagg
     1561 ccagcgccgg caccgacatc gggacgatca gccgccagaa ggtcttgaag tggctggccc
     1621 cgtcgatctt ggccgactcg atcaccgcct tgggcagcgt ggacatgtag ttgcggagga
     1681 tgtagacggc cagcggcatg ccgaagccga tgtgggccag gtagacggcg gcgaactgcc
     1741 ccgtcagccc gacgtcgccg tagaagcgca gcagcggcac cagcgcgacc tggttgggca
     1801 ccaccaggag ggcgacgatg accacgaaca gcacgtcacg gaacgggaac tgcatgaagg
     1861 tgaacgcgta ggccgcgaag gcggcgatca tgatcgggat caccgtcgcc ggcagggtca
     1921 ccgcgaagct gttgatgaag gcgttgccca tcccctcgtt ccaggcctgg ctgtagttgg
     1981 cgagggtgta ctgggtgaag tcgagcggcg aggtcaagac cgtccaccag cccgaggtgt
     2041 tggccgcgtc cgcggtgcgg aaggcggtga tgacgatccc cagcgtcggc acgatccaca
     2101 ggaagcagag gatgaccagg gcgagcctga ccacccagtt ggcctgcccg gcgccgggag
     2161 cggccccgcc ctcgcggcga gggatggccc ggcgcggctt gacctcgccg gggaccgggg
     2221 tcggaggagg agtggttccg gtggtcgtca tcgtgctgcc tc
//
LOCUS       DASSWR010000631         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_617436,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000631 DASSWR010000000
VERSION     DASSWR010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617436"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFO47_10545"
     CDS             complement(<1..410)
                     /locus_tag="VFO47_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8789175.1"
                     /translation="MPIDPAMLAKSIATLTDLDPERDLAATLDQAVVAAKQLFAVDAA
                     GIMLADADGKLRWASASDLLAQTLEDNQETFAAGPCLEAFTTGRPAVMREATLEPRWG
                     EITLAFVELQIRSGLSVPVELGGGPIGTLDVYNAA"
     gene            1172..1636
                     /locus_tag="VFO47_10550"
     CDS             1172..1636
                     /locus_tag="VFO47_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789176.1"
                     /translation="MRAPVPVRLLLFAMVCLAAACGGSGDDTSDSAPATTTTAASGTT
                     AASQACADAAALKASMAELDQLDPPTAGKAGIQAALDEARTNLAALKTSANSQWGSQI
                     TELDGAVEALKTTIAGINGDSLLQDLPTIVSDLGRIDTAWTALQQQIDQDCG"
     gene            complement(1873..>2432)
                     /locus_tag="VFO47_10555"
     CDS             complement(1873..>2432)
                     /locus_tag="VFO47_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789177.1"
                     /translation="EAALPAAGWGPDQPVRLVVATTDPATLPKLTTWYLVSNLPRPGQ
                     RACGPFPPAGLAELVRLYGLRNWVEQGYKQVKGELGWADFQVRSDRAIRRHWALVCCA
                     FSFCWQAFLAEHPDRPAPADPQAVPTAARGAQATGHRGGTGRRSVVAEGVASGAGVAD
                     PLERAGALLAVVVTSAAAPAAATAA"
BASE COUNT          381 a          872 c          823 g          356 t
ORIGIN      
        1 gcggcgttgt acacatctaa ggtcccgatc gggccaccgc ccagctccac cggaacactg
       61 aggcccgacc ggatctgcag ctcgacaaac gccagggtga tctcgcccca gcgcggctcc
      121 agggtggcct cgcgcatgac cgccggccgg ccggtggtga acgcctccag gcaggggcca
      181 gcggcgaatg tctcctggtt gtcttccagg gtctgggcga gcaggtcgga ggcgctggcc
      241 caccgtagct tgccgtcggc gtcggcgagc atgatcccgg cggcgtcaac cgcgaacagc
      301 tgcttggccg cgaccaccgc ctggtccagg gtggcggcca ggtcccgctc ggggtccagg
      361 tcggtcaggg tggcgatgct cttggccagc atggccgggt cgatcggcat gggcgtgtgc
      421 ctttggttcc ggtcgccgaa ctggcgaacc cggtcaacag gccaggcgaa cccatcctgg
      481 ggtgccgatc aggcccaaca gccccacggt gtcgtcatgt gggcttcggc tgggtcctgt
      541 gacggcaaca acaagaacca gccctgtgag catacgcctt tggggcggcg gccgtcctcg
      601 gccggacggg atcccatccg ggtgatgcca caggagtgaa tgtggagtga gatgaccgcg
      661 gcggtcgcca cggccaccgt cgttggtgcc gctgctgctg tccggcaaca gctatgagcg
      721 ttctgtacga acgcaaaggg gatgcggcca tgtccgtcat ctggatcgca atcgcgttct
      781 ggcccgcccg tgtcgctagc cgaaagggcc acagcttcat cgggtacttc atcttcagcc
      841 tcgtcttctt cccggccgcg ctcctcgtcg cgtacctcgt cccggaccgc acccaagtcg
      901 accggaccca actcccaggc gggagtacga cagctgtcag ccaaaggggg cgaccagacg
      961 ttgtgcctgt aatcgagtga aggtcgctga ttgcagtagc ctcgcgggtc gcggaagcct
     1021 tccacagcac caccgacccg acgaggccgc cgccagtctg ggttccccaa cgtgttcgcc
     1081 aaccccacct gtccagccgg ctgtcccggc gacctgctgc cggcactgca cggcccaccg
     1141 cgtcgccgtc cgcttggtca tggaggacat catgcgtgcc cccgtccccg tcaggctcct
     1201 gctcttcgcc atggtttgtc tcgccgccgc ctgcgggggc tccggtgacg acaccagcga
     1261 cagcgctcca gccaccacca ccaccgcggc gagcgggacc acggcggcgt cccaggcctg
     1321 tgccgacgcg gccgcgctga aggcgtcgat ggccgagctc gaccagctcg acccccccac
     1381 ggccggcaag gccgggatcc aggccgccct ggacgaggcc aggaccaacc tggcggcgct
     1441 caagaccagc gccaacagcc agtggggcag ccagatcacc gagctggacg gtgccgtgga
     1501 agccctcaag acgaccatcg ccgggatcaa cggcgacagc ctgctccagg acctgccgac
     1561 aatcgtcagc gacctggggc ggatcgacac cgcctggacg gcgctgcagc agcagatcga
     1621 ccaggactgc ggctagaaga ccgtggccag tgcctgacct ccagcgtgag attgctcccc
     1681 tcggctggct ggccacccgg ctcgccggaa ggctccggca cgaacgtcag cggtgctacc
     1741 tgctcgcccc agccagacct gtcactcgga ccgctgctgg ggctgtccca ggcggcagtc
     1801 ctacgaggct atcggtagtt tgttgactat ggtggtaggt agaggtgcaa tggctggccg
     1861 gcggcagcgg cgtcaagcag ccgttgcagc tgccggggcg gcggcgctgg tgaccacgac
     1921 cgccagcagc gcgccagcac gctccagggg atcagccacg cctgcaccgc tcgcaacgcc
     1981 ctcggccacg acagacctgc ggccggttcc gccccgatga ccggtcgcct gcgccccccg
     2041 cgcggccgtc ggaacggcct gggggtcggc tggtgctggc cggtcgggat gctcggcgag
     2101 gaaggcctgc cagcagaagc tgaacgcaca gcacaccagc gcccagtgcc gccggatcgc
     2161 ccggtcggat cggacctgga agtcggccca gcccaactcg cccttgacct gcttgtagcc
     2221 ctgctcgacc cagttgcgca gcccgtacag gcgcaccagc tcggccaggc cggcgggcgg
     2281 gaatggaccg catgcccgct ggccggggcg aggcaggttg ctgaccaggt accaggtggt
     2341 cagcttcggg agcgtggccg ggtcggtggt cgccaccacc agccgaacgg gctggtccgg
     2401 accccagccg gccgccggca gcgccgcctc gg
//
LOCUS       DASSWR010000632         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1323628, whole genome shotgun sequence.
ACCESSION   DASSWR010000632 DASSWR010000000
VERSION     DASSWR010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323628"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..802)
                     /locus_tag="VFO47_10560"
     CDS             complement(143..802)
                     /locus_tag="VFO47_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HET8789178.1"
                     /translation="MNDKQRFPPAAVGSLAGGGEAPLVAAAAHALAASVDPRTVVLVE
                     GISDQVALQALAARRGRELAAEGVAVVPMGGARNIRRFLELFGSQGLNRGLAGLCDAG
                     AEGDFRRGLERAGLGSSLGRADMEALGFYVCVADLEDELIRCLGAAGVEQVVEAQGEL
                     GSFRTFQQQPAWQGRSRQQQLRRFIGTHSGRKIRYARLLVDALELTSVPRPLDRVLAH
                     S"
     gene            complement(807..2021)
                     /locus_tag="VFO47_10565"
     CDS             complement(807..2021)
                     /locus_tag="VFO47_10565"
                     /inference="COORDINATES: protein motif:HMM:NF037017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET8789179.1"
                     /translation="MTRSRRPGAPRPLLPLVAAAVAVVALAAGGCTAGDRPAPAGTAR
                     EAGGGEVAAPVKVTVTPRDRATRVGLDTRARVVADAGRLRAVRVTTSGGRKLAGTLAD
                     DGRTWVSTGPLAPATRYRVVAEAVNDAGAPTRARTSFTTLRPRAELRAAIMPLDGETV
                     GVGLPIGVWFNQPVADRAAVERRLEVTSSKPVTGAWHWFADNEVHYRPKSYWPSGSRV
                     TLRARLAGADAGRGVWGVADRTIRFRIGERRVSIVDVRTHRMKVVSGGRTLRVLPVST
                     GRERYPTTNGVHFVLEKTPVKLMDSSTVGIPRNSPGGYYQRVAWSVRISNSGEFVHAA
                     PWSTGSQGRANVSHGCVNLSTANAAWFYRLTRRGDVVEVRGSPKRPGNSFGVADWNMS
                     WSRWLAGSALPG"
     gene            2182..>2416
                     /locus_tag="VFO47_10570"
     CDS             2182..>2416
                     /locus_tag="VFO47_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET8789180.1"
                     /translation="MRRAIIVVLALALLLPAGVLARGAGADPVDLPGLEGPASVDRDA
                     NGIAHIRAGSRHDLFFLQGWVHAQDRLFQMDVRR"
BASE COUNT          313 a          914 c          884 g          305 t
ORIGIN      
        1 ggtcaggtcc atcgtgaggg cattgtaggc ggcgaccgcg ccgccaaccc ggcatgatca
       61 ggcgtcctag gcgcaccaga cagtcctatg cgcacgcaac cccgcgcccg ctcggaagca
      121 aggcggaggc cttgttcgag cgtcagctat gggccaggac gcgatcgagc ggccgaggca
      181 cgctcgtgag ctccagcgcg tcgacgagca accgcgcgta gcggatcttg cggccgctgt
      241 gggtgccgat gaagcgccga agctgctgct ggcggctccg tccctgccag gcgggctgct
      301 gctggaaggt gcggaacgat ccaagctcgc cctgggcctc gacgacctgt tccacaccgg
      361 cggcacccag gcagcggatc agctcgtcct ccaggtcggc gacgcacacg tagaacccga
      421 gcgcttccat gtcggcgcga ccgaggctgg agccgaggcc ggcccgctcc agaccgcgcc
      481 ggaagtcgcc ctccgcaccg gcgtcacaca ggcccgccag tccgcggttc aagccctggg
      541 aaccgaacag ctccaggaag cgtcggatgt tcctggcccc gcccatcggc accacggcaa
      601 cgccctcggc ggcaagctct cgaccacggc gcgcagccag cgcctggagc gcgacctggt
      661 cgctgatccc ctcaaccagc acgaccgtcc gtgggtcgac gctggcggcc agggcgtgtg
      721 ctgccgcggc gaccagcgga gcctctccgc cgccggccag cgacccgacg gctgccggcg
      781 ggaatcgctg cttgtcgttc acctgcctag ccgggaaggg cgctgccggc cagccagcgg
      841 ctccaggaca tgttccagtc ggccacgccg aagctgttgc cggggcgctt gggcgagccc
      901 cggacctcga ccacgtcgcc gcggcgggtc aggcggtaga accaggccgc gttggcggtg
      961 gagaggttga cgcagccgtg ggagacgttg gcccggccct gggagccggt cgaccagggg
     1021 gcggcgtgga cgaactcgcc ggagttggag atccgcaccg accaggcgac ccgctggtag
     1081 tagccgcccg gggagttccg ggggatgcct acggtcgagg agtccatcag cttcaccggg
     1141 gtcttctcga ggacgaagtg gaccccgttg gtggtcgggt accgctcccg gccggtgctg
     1201 accggcagga ccctgagggt gcgcccgccc gagacgacct tcatgcggtg ggtccggacg
     1261 tcgacgatgc tgacccggcg ctcgccgatg cggaagcgga tcgtccggtc ggccacaccc
     1321 cagaccccgc ggccggcgtc ggccccggcc agccgggccc gcagggtgac ccgggacccg
     1381 ctcggccagt agctcttcgg ccggtagtgg acctcgttgt cggcgaacca gtgccaggcc
     1441 ccggtcaccg gcttggacga ggtcacctcc aggcggcgct cgaccgcggc ccggtcggcc
     1501 accggctggt tgaaccagac gccgatgggc aggccgaccc cgaccgtctc gccgtccagg
     1561 ggcatgatcg ccgcccgcag ctcggcccgg ggccgcaggg tggtgaagct ggtccgcgcg
     1621 cgggtcgggg ccccggcgtc gttgacggcc tcggccacca cccggtagcg ggtcgccggg
     1681 gccagggggc cggtgctcac ccaggtgcgg ccgtcgtcgg ccagggtgcc ggccagcttc
     1741 cgcccgccgc tggtcgtgac ccggacggcc cgcagccgcc cggcgtcggc caccacccgg
     1801 gcccgggtgt cgagcccgac ccgggtcgcc cggtccctgg gggtcacggt gaccttgacc
     1861 ggggccgcca cctcgccccc gccggcctcc ctggccgtcc cggccggggc cggccggtcg
     1921 cccgcggtgc agccgccggc ggccagggcg accacggcga ccgcggccgc gaccagcggc
     1981 agcagcggcc gcggtgctcc gggacggcgc gaacgagtca tcgccctccg agtctacccg
     2041 gccctccggt cccggcccgg gtgcgagcgc gtcccggact tgaacagacg caccgctttg
     2101 gtagtcttcg ccgggttcgg acgacgcccg gggcagcggt cgctccggga gctgggagcc
     2161 gtcccaggag agatcacccc gatgcggcgc gccatcatcg tcgtcctcgc cctcgccctg
     2221 ctgctgccgg ccggggtgct ggcccgcggg gccggcgccg acccggtcga cctgcccggg
     2281 ctggaggggc cggcctcggt cgaccgcgac gccaacggca tcgcccacat ccgggccggc
     2341 tcccgccacg acctgttctt cctccagggg tgggtccacg cccaggaccg gctgttccag
     2401 atggacgtgc gccgcc
//
LOCUS       DASSWR010000633         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1154016, whole genome shotgun sequence.
ACCESSION   DASSWR010000633 DASSWR010000000
VERSION     DASSWR010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154016"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="hutH"
                     /locus_tag="VFO47_10575"
     CDS             complement(<1..1260)
                     /gene="hutH"
                     /locus_tag="VFO47_10575"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET8789181.1"
                     /translation="MTRRGEEGGPPGRVVLTGGPLQVAEVVAVARGQVPVELGEEGRK
                     RMAAARNVVERAVAEGRRVYGVSTGFGLLANTAVDPADLEELQRRIVLSHATATGDPL
                     DVEVVRGMQLLRARTLTQGFSGVRPAVVEALVALLAAGVTPVIPEHGSVGASGDLAQL
                     AHLALPLLGEGEVMVDGRRVPAAEGLRLAGLAPLRLSFKEGLSLVNGTEGMLALACLA
                     VADAEALAAMADVACAMSIEGLLGTDRPFQARLHELRPHPGQVASAANLRTMLAGSPI
                     LKSHRHSDHAIQDAYSLRCAPQVHGACRDVVAFARQVVERELGSVTDNPVVFAETDEV
                     ISVGNFHGEPLAFVLDFLAIALAELADISERRVDRLLDPALNHDLPPFLARDPGLNSG
                     LMLTQYTAAALVADCKVLAHPASVDSIP"
     gene            1512..1739
                     /locus_tag="VFO47_10580"
     CDS             1512..1739
                     /locus_tag="VFO47_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789182.1"
                     /translation="MGTDDSGTATPTLQREVCDKCGSRMELERSAYGFERWKCPKCQQ
                     IVGIDRDPEVGSRFQIARGQPWNYSPDAFKN"
     gene            complement(1773..2486)
                     /locus_tag="VFO47_10585"
     CDS             complement(1773..2486)
                     /locus_tag="VFO47_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET8789183.1"
                     /translation="MPKLVHHPSDHPPPEAPNRPGWVPRRPGPDGGARPAWALPAALV
                     AMALVYPAAFFFQVVPFWSAMLAFSAFSWWAAWHPYLRTRLRPTRRLVVMGLISGVAL
                     YLCFCAGALVVRATPLWPRIQEVVDLTRTTAPGAAAALVIVFATSPSEEVLWRGAVFA
                     RLTRRYGPGWRPLVATTVLYALFVGLSGSLVLPLAALICGAVWTRQRQVTGSIVPSLV
                     SHALWSLLIFLYIPGLGGL"
     gene            2590..3279
                     /locus_tag="VFO47_10590"
     CDS             2590..3279
                     /locus_tag="VFO47_10590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8789184.1"
                     /translation="MPETVIHLVRHGQVENPRRVLYGRLPGYHLSSRGQAQADLLAGH
                     FAGAPVAAVVASPLERAQQTAAPIAATHGLEVRTDLRLIETSNIFEGVAGNVAWYIVR
                     HPRLWWKLRDLRAPSWGERNVDMVERVHAVVDAVREEFAGRQVVLVSHQAPIWVARLA
                     FERRRLTHWPGFRRCTLASVTTLTFDGDQLAGVSYAEPAASALLGPAPGATGTPRPGP
                     EDGLRPEREAQ"
     gene            complement(3253..4377)
                     /locus_tag="VFO47_10595"
     CDS             complement(3253..4377)
                     /locus_tag="VFO47_10595"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HET8789185.1"
                     /translation="MHQAEIAQVFQADGPFTSVYMHTEGDVEQAADRIALRWKNLRGA
                     MLAAGVPERTVAAIDPLVEGSHMAGATLAVIAAVDGPRYSANLPDPPPRETLLRHGAL
                     PDVLPLLEAGQAAVAHVAVLTDRTGADMAARVAEDVARTERVSGRVTPHLHKPQAGGW
                     SQPRFHHRAEAIWESNAQEVADALTRLVDQVRPRFVAAAGDVRALQLLQEQAPKRVRE
                     LLTVVGGEHGSLEAVFAEADKLVAATVEADNRALLDRFAEELGQAAAGAGNRAVEGAA
                     ATLAALGRGQVETLLLTGLFLDDERTAWFGPAPTDVGADRDALAGLGVPGPVEGRLVD
                     VAVRAALGTGAGVRVLEADDRSRHAPGEGLGALLRFPLGP"
     gene            complement(4400..>4859)
                     /locus_tag="VFO47_10600"
     CDS             complement(4400..>4859)
                     /locus_tag="VFO47_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET8789186.1"
                     /translation="EVVNEQVADRLGLGEADLRRAAEAEGRELARRERSYREGRPPPD
                     LGGRVVILVDDGLATGSTMRAAVAAVRRLGPARVVVAVPTAPASTCQRLAEEADEVIC
                     ATTPRPFRAVGYSYRSFPQTSDEEVRTLLQAAWAGAPSPDAPAGGGRTPT"
BASE COUNT          652 a         1826 c         1824 g          557 t
ORIGIN      
        1 cgggatcgag tccaccgagg ccgggtgggc cagcaccttg cagtccgcga ccagggcggc
       61 cgccgtgtac tgggtcagca tcaggcccga gttgaggccc gggtcgcggg ccaggaacgg
      121 gggcaggtcg tggttgaggg cggggtcgag cagccggtcg acccggcgct cggagatgtc
      181 ggccagctcg gccagggcga tggccaggaa gtcgagcacg aaggcgagcg gctcgccgtg
      241 gaagttgccg acggagatga cctcgtcggt ctcggcgaac accaccgggt tgtcggtgac
      301 cgagcccagc tcccgctcga ccacctggcg ggcgaaggcg accacgtccc ggcaggcccc
      361 gtggacctgg ggggcgcagc gcagcgagta ggcgtcctgg atggcgtggt cggagtggcg
      421 gtggctcttc aggatgggcg acccggccag catggtccgg aggttggccg cgctggccac
      481 ctggccggga tgggggcgca gctcgtgcag ccgggcctgg aagggccggt cggtgccgag
      541 cagcccctcg atcgacatgg cacaggccac gtcggccatg gcggccaggg cctcggcgtc
      601 ggccacggcc aggcaggcca gggcgagcat cccctcggtg ccgttgacca gcgacaggcc
      661 ctccttgaag gacagccgca gcggggccag cccggccagg cgcagcccct cggcggcggg
      721 gacgcggcgg ccgtcgacca tgacctcgcc ctcgccgagg agcggcaggg ccaggtgggc
      781 gagctgggcc aggtcgccgg aggcgccgac cgacccgtgc tcggggatga ccggcgtgac
      841 cccggcggcg agcagagcga ccagcgcctc caccaccgcc ggccgcaccc ccgagaaccc
      901 ctgggtgagg gtgcgggccc gcagcagctg catgcccctg accacctcga cgtcgagtgg
      961 gtcgccggtg gcggtggcgt gcgacaggac gatccggcgc tgcagctcct ccaggtcggc
     1021 ggggtcgacg gcggtgttgg cgagcaggcc gaacccggtg gagacgccgt agacccgccg
     1081 gccctcggcc accgcccgct cgaccacgtt ccgggccgcg gccatccgct tgcggccctc
     1141 ctcgcccagc tccaccggga cctggccgcg ggccacggcc accacctcgg cgacctgcag
     1201 cggcccgccg gtcaggacca cccgccccgg cggcccgccc tcctcgcctc gtcgcgtcat
     1261 gcccgcatcg taggtcggcc ccggacgcgc cgtcacgcct ccgtgatagt tctggacccg
     1321 gattgcccag gcgaccatcc gggtatccag ggaagacacg acgaagcttg cctaggtagc
     1381 cgccgtagcc agccggcgga taccagatcg gtatgcggaa gttgcccgcg aagggatgca
     1441 agatcgtctg agttaggcca tgctggtggg gcgtcccggc gtcgaccggg gggagcaagg
     1501 agacgacgac catgggcacc gacgacagcg gcaccgccac cccgaccctg cagcgcgagg
     1561 tctgcgacaa gtgcggctcg cgcatggagc tggagcgctc ggcctacggg ttcgagcgct
     1621 ggaagtgccc gaagtgccag cagatcgtcg gtatcgaccg cgaccccgag gtcggcagcc
     1681 gcttccagat cgcccgcggc cagccctgga actactcccc ggacgccttc aagaactgac
     1741 gatcgcccag cgggcaggtt gatcccgggt cgtcagaggc caccgagccc tgggatgtac
     1801 aggaatatca gcagcgacca cagggcgtgc gacaccaggc tgggcacgat gctccccgtg
     1861 acctgccgct gcctcgtcca cacggcgccg cagatcaggg cggccagggg caggaccagg
     1921 ctgcccgaca ggccgacgaa cagcgcgtac agcacggtgg tggcgaccag cggccgccat
     1981 cccggcccgt agcgcctggt caggcgggcg aacaccgctc ctcgccagag cacctcctcg
     2041 ctcggcgagg tcgcgaacac gatcaccagg gcggcggccg cgcccggggc ggtcgtccgg
     2101 gtcaggtcga cgacctcctg gatgcggggc cacagcgggg tcgccctgac caccagcgcc
     2161 ccggcgcaga agcacaggta gagggcgacg cccgagatca ggcccatgac gaccagccgc
     2221 ctggtcggcc gcagccgggt gcggaggtac gggtgccagg ccgcccacca ggagaaggcc
     2281 gagaaggcca gcatggccga ccagaacggc accacctgga agaagaaggc ggccgggtag
     2341 accagggcca tggcgaccag ggcggccggc agcgcccagg ccggccgggc cccgccgtcg
     2401 ggaccgggtc gccgcggcac ccagcccggc cggttcggcg cctccggggg cgggtggtcg
     2461 ctcgggtggt gcacgagctt cggcatagcg gcagcatagg cgggccccgg ccacaccgcc
     2521 cctcgaggcc gcggccggag ccgctgctac catctgcccg ccgtcccaga gagctgtgag
     2581 gatcagcgcg tgcccgagac cgtcattcac ctggtccgtc acgggcaggt ggagaatccc
     2641 cgccgggtgc tctacggccg gctgcccggc taccacctgt cgagccgcgg ccaggcccag
     2701 gccgacctgc tggccggcca cttcgccggc gcgccggtgg cggcggtggt ggccagcccc
     2761 ctggagcggg cccagcagac ggccgccccg atcgccgcca cccacggcct cgaggtccgc
     2821 accgacctgc gcctcatcga gacgagcaac atcttcgagg gggtcgccgg gaacgtcgcc
     2881 tggtacatcg tccgccaccc caggctgtgg tggaagctgc gcgacctgcg cgccccctcc
     2941 tggggcgagc gcaacgtcga catggtcgag cgggtccacg ccgtggtcga cgccgtccgg
     3001 gaggagttcg ccggccgcca ggtcgtgctc gtctcccacc aggccccgat ctgggtggcc
     3061 aggctggcct tcgagcgccg ccgcctcacc cactggcccg gcttccgccg ctgcacgctg
     3121 gccagcgtga ccaccctcac cttcgacggc gaccagctgg ccggcgtcag ctacgccgag
     3181 ccggccgcgt ccgccctcct gggccctgcc cccggcgcca ccgggacgcc gcggcccggc
     3241 cccgaggacg gcctacggcc cgagcgggaa gcgcagtagg gcgcccaggc cctcgccggg
     3301 ggcatggcgc gaccggtcgt ccgcctccag cacgcgcacg ccggcgccgg tccccagggc
     3361 ggcccggacg gccacgtcga ccagccggcc ctccaccggg ccgggcacgc cgagcccggc
     3421 cagggcgtcg cggtcggcgc cgacgtcggt cggggccggc ccgaaccagg ccgtccgctc
     3481 gtcgtcgagg aacaggccgg tgagcagcag cgtctcgacc tggccgcggc cgagggcggc
     3541 cagggtcgcg gcggcgccct cgaccgcccg gttgccggcc ccggcggcgg cctggcccag
     3601 ctcctcggcg aaccggtcaa ggagcgcccg gttgtcggcc tcgacggtgg ccgcgaccag
     3661 cttgtcggcc tcggcgaaga ccgcctccag ggagccgtgc tcgcccccga ccaccgtgag
     3721 cagctcccgc acccgcttgg gcgcctgctc ctggaggagc tggagggcgc ggacgtcccc
     3781 ggcggcggcc acgaacctgg gccgtacctg gtcgaccagc cgggtcaggg cgtcggccac
     3841 ctcctgggcg ttggactccc agatggcctc ggcccggtgg tggaaccgag gctgcgacca
     3901 cccgccggcc tgcggcttgt ggaggtgggg ggtgacccgc ccggagaccc gctcggtcct
     3961 ggccacgtcc tcggcgaccc gggccgccat gtcggccccg gtccggtcgg tcagcacggc
     4021 gacgtgcgcg accgccgcct gcccggcctc cagcagcggg agcacgtccg gcagggcgcc
     4081 gtggcgcagc agcgtctcgc ggggcggcgg gtccgggagg ttggccgagt agcgcgggcc
     4141 gtcgacggcg gcgatcacgg ccagggtcgc tccggccatg tggctcccct cgaccagcgg
     4201 gtcgatcgcg gcgacggtcc tctcggggac cccggcggcc agcatcgccc cgcgaaggtt
     4261 cttccagcgc agggcgatcc ggtcggcggc ctgctcgacg tcgccctcgg tgtgcatgta
     4321 cacggaggtg aacggcccgt cggcctggaa cacctgggcg atctcggcct ggtgcatggt
     4381 cctcctcagc tcgatcggtt caggtcgggg ttcgcccgcc gccggcgggg gcatcggggc
     4441 tcggggcgcc ggcccacgcc gcctggagga gcgtcctgac ctcctcgtcg ctggtctggg
     4501 ggaacgaccg gtaggagtag ccgaccgccc ggaacgggcg cggggtggtg gcgcagatca
     4561 cctcgtcggc ctcctccgcc aggcgctggc aggtggaggc gggcgcggtc gggaccgcga
     4621 ccaccacccg ggccgggccc aggcggcgga cggcggccac cgccgcccgc atggtcgagc
     4681 cggtggccag gccgtcgtcg accaggatca cgacccggcc gccgaggtcg ggcgggggcc
     4741 ggccctcccg gtagctacgc tcgcgcctgg ccagctcgcg gccctcggcc tcggccgccc
     4801 ggcgcaggtc ggcctcaccc agcccgaggc ggtcggccac ctgctcgttg accacctcg
//
LOCUS       DASSWR010000634         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_673893,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000634 DASSWR010000000
VERSION     DASSWR010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673893"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..377)
                     /locus_tag="VFO47_10605"
     CDS             complement(132..377)
                     /locus_tag="VFO47_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HET8789187.1"
                     /translation="MLARLATAALVGLTAYPVGVEVDIGRGLPAVTVVGLGGTAVLEA
                     RDRLRAAFGNTGYEWPDRRITVSLPRPTCPSRAPGST"
     gene            complement(380..751)
                     /locus_tag="VFO47_10610"
     CDS             complement(380..751)
                     /locus_tag="VFO47_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET8789188.1"
                     /translation="MPVRHPADRGELGRRGEALACARLTAAGLRIVARNWRCRTGEID
                     VVAEGPGLLVFCEVKTRRGDGYGTPAAAVNAAKQAQLRRLAGAYLRATPHRPCKVRFD
                     VVTVVWPRSGQPRVEHLKGVL"
     gene            1681..2064
                     /locus_tag="VFO47_10615"
     CDS             1681..2064
                     /locus_tag="VFO47_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789189.1"
                     /translation="MENMGALSSRRDVFLDERGAGLRVTWHPERDLVVLSVWQGDSCV
                     GTFRMLVQDVPRLSGLLAAALGDWVGETGPGASMGPGAGVGPGAGVSPEPGAGGARLS
                     VQQRLRLSRPVLKLRGLRHRGRGSA"
     gene            complement(2114..>3288)
                     /locus_tag="VFO47_10620"
     CDS             complement(2114..>3288)
                     /locus_tag="VFO47_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET8789190.1"
                     /translation="YGDVGGQRNDTTLVVRVEPARKRVSMLSIPRDLVVPIAGTGVEN
                     RINAAFTEGPGQLVKTIEESFGIPIHHYVLIDFDGFRAIVDALGGIDVRFPYPSRDAK
                     SGLDIREAGCRHLDGGRALALARSRYFAYQLDGVWHSDPWADLGRIRRQQVFLQAVTK
                     AALDKGLTNPVRANAFVGSLVHEVTKDSGLRVSDVIRTGAAFRSFSPSKLATYTVPVT
                     VPSDHPLGDVLLLKQPDTDTVVGRFLGRATPAPSATPGAAAPASTQVTVLNALGTTGL
                     AATTATKLRTSGYQIATVGNAPSADLARSQISYAPGRLADAKTLAGTLLGGARLIEDP
                     TLAPSHLTLILGPGFTGIRPLTKGGPATTTGSDTKAKKKKAEKALPDLRPYDPRPC"
BASE COUNT          394 a         1071 c         1380 g          443 t
ORIGIN      
        1 cggcagtcgg tctcctgccg ctccacgccg agcccggcgc cggtccgatc ctgggagatc
       61 cgggtgtaga tggccgctcg gcggcgcgag gtctccatcc ccgaatctta gcatggctca
      121 ggttgacttc gctaggtcga acccggagcc ctgcttgggc aggtcggccg ggggagggac
      181 acggtgatcc gccggtccgg ccactcgtag ccggtgttgc cgaacgcggc ccggagccgg
      241 tcgcgcgcct ccagcacggc cgtcccgccg agcccgacga cggtcacggc gggcaggccg
      301 cggccgatgt cgacctcgac cccgaccggg taggcggtga ggccgaccag cgcggccgtg
      361 gccagccggg ccagcatcgt cagagcaccc ccttcaggtg ctcgacccgc ggctggcccg
      421 agcgcggcca gacaacggtc acgacgtcga agcggacctt gcagggccgg tgcggggtgg
      481 cacggaggta ggccccggcg agcctgcgga gctgggcctg cttggcggcg ttgacggcgg
      541 ccgccggggt cccgtagccg tcgccacgcc tggtcttgac ctcacagaac accagcagcc
      601 cgggaccctc ggcgaccacg tcgatctcgc ccgtccggca gcgccagttg cgggccacga
      661 tccgcagccc ggccgcggtc agccgggcgc aggcgagcgc ctcgccgcgc cgccccagtt
      721 cgccccgatc ggcggggtgc ctgacaggca tggccgcacc ctagccgccg gcccccgcga
      781 attggacgac tccgccaaga atgtggaaaa ccccgcgcaa atgcatccgg cggctgggga
      841 tgcgtggacc cgatgcgtcg gcgggtgccg gccgtgtgcc tgctcctggc cggcgccgcc
      901 gtggccggtg acgacggtgg tcggccgggc cgccatcgga gacgacggca cggggcgcgg
      961 tgacgaccac gccaaggggg cgagctgccg gcgggctccc tcgacgacac gctcgacctg
     1021 gtccggcagc aggcgctcta cgggtcgact ggccggccgt gcgggtcgag gcccggcggc
     1081 gggccggcgc ctcggcgtcc gggacggcga cctacgacgc cgtccgctgg gtgcgcttcg
     1141 agctcggcga ccgccacagc ttcctgctca ctcggaggag gcgcggacgc tgtcggccgg
     1201 acagggggct cggcttcggc tcgtcgcgct gttccccgag cgggtggtcg tggacgtcga
     1261 gccgggtggc ggggcggctc gggcgggggt gcgggtcgag ggcgacgagg tcgtcaggcc
     1321 gccgccggcc gaggtgggcg gccagccggc gcgggtggtg ctcgccctgc ggcgcggagc
     1381 cggcgaccgg tcccgccggc tccgggtcac ggtcgaggcg gccgaggcgc cggccgtacg
     1441 gccggtgtcc ccaccgggaa cgcccagcat cgcctgccgg acggggtcga gctgtacctc
     1501 accgagggga tcggggtcga cggcacgggt cgcgcccccg gcccgaccag ccggtggcga
     1561 ccgactggac ccggtacgcc acctcggccg acccggtgct ggaggccgcg accgagtggc
     1621 tggccgggcg ctgcggccgg aggtcttgaa accggggccg ggagcgccca cgctaggcct
     1681 gtggagaaca tgggagcgtt gtcgtcccgc cgggatgtgt tcctcgacga acgcggggcc
     1741 ggattgcgcg tcacctggca tccggagcgg gacctggtgg tgctgagcgt ctggcagggc
     1801 gacagctgcg tcgggacgtt ccggatgctg gtccaggacg tcccccggct cagcggcctg
     1861 ctggcggccg ccctcggcga ctgggtcggc gagaccggcc ccggcgccag catgggcccg
     1921 ggtgcgggtg tgggtccggg ggcgggtgtg agtccggaac ccggggccgg tggggccagg
     1981 ctgtcggtgc agcagcggct caggctcagc aggccggtgc tgaagctgcg cgggctgcga
     2041 catcggggcc gagggtccgc ctagttcccc cggaactcca gccgccaggc ccccgacccc
     2101 gccgtgaccg gtctcagcag ggtcgtgggt cgtagggccg caggtccgga agggccttct
     2161 ccgccttctt cttcttggcc tttgtgtccg aaccggtggt tgtggccggc ccacccttgg
     2221 tcagggggcg gatgccggtg aagccggggc cgaggatgag ggtgaggtgg gaaggggcga
     2281 gggtggggtc ctcgatgagg cgggcgccgc cgaggagggt gccggcgagg gtcttggcgt
     2341 cggcgaggcg gccgggggcg tagctgatct gggatcgggc gaggtcggcg gagggggcgt
     2401 tgccgacggt ggcgatctgg tagccggagg tgcggagctt ggtggcggtg gtggcggcca
     2461 ggccggtggt gccgagggcg ttgaggacgg tgacctgggt gctggcgggg gcggcagcgc
     2521 cgggggtggc gctgggggcc ggggtggcgc ggccgaggaa gcggccgacc acggtgtcgg
     2581 tgtcgggctg cttgaggagc aggacgtcgc cgaggggatg gtcgctgggg acggtgaccg
     2641 gcacggtgta ggtggccagc ttggaggggc tgaaggagcg gaaggcggcg ccggtgcgga
     2701 tgacgtcgct gacccgcagg ccgctgtcct tggtgacctc gtggaccagg gagccgacga
     2761 aggcgttggc ccggaccggg ttggtcaggc ccttgtcgag ggcggccttg gtcacggcct
     2821 ggaggaacac ctgctggcgg cggatgcggc ccaggtcggc ccacgggtcg ctgtgccaca
     2881 ccccgtcgag ctggtaggcg aagtagcggc tgcgggccag ggcgagcgcc cggccgccgt
     2941 cgaggtggcg gcagccggcc tcgcggatgt cgaggccgga cttggcgtca cgcgaggggt
     3001 aggggaagcg gacgtcgatg ccgccgaggg cgtcgacgat ggcccggaag ccgtcgaagt
     3061 cgatcagcac gtagtggtgg atggggatgc cgaagctctc ctcgatggtc ttgaccagct
     3121 ggccggggcc ttcggtgaag gcggcgttga tgcggttctc gacgccggtg ccggcgatcg
     3181 ggacgaccag gtcgcggggg atggacagca tggagacgcg cttgcgggcc ggttcgaccc
     3241 tgaccaccag ggtggtgtcg ttgcgctggc cgccgacgtc gccgtagc
//
LOCUS       DASSWR010000635         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_81405,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000635 DASSWR010000000
VERSION     DASSWR010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81405"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1099
                     /locus_tag="VFO47_10625"
     CDS             185..1099
                     /locus_tag="VFO47_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HET8789191.1"
                     /translation="MDADLDTLATALYVKIDELLQQAPQLAPWRPTVGIAPKLSDAEL
                     VTLAVMQALLGFTSEARWLRHADRHLRHLFPYLPRQAGYNKRLRGAAGLLRHAIRAVA
                     TDTSLWTDDIWIVDSTPVECGRSRETTKRSALAGWAQYGYCASHSRYFWGLRLHLVCT
                     LHGLPVAFALTGAKADERQVLVDLLAVEPELVAARPGQTLLADKHYYGREFQAILAGL
                     GVRLLRPARKGEPQRAGAHLFCPLRQLIESVNQTFKGQLDLERHGGRTCGGVAVRVLQ
                     RILALAVAIWHNHKTGQPTLRSLIAYDH"
     gene            complement(1132..2604)
                     /locus_tag="VFO47_10630"
     CDS             complement(1132..2604)
                     /locus_tag="VFO47_10630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET8789192.1"
                     /translation="MAIDFHAIAPELVLAGTALLVLVVDLFVERAKVVASWLSIAGTV
                     AAGIALASLVGGGTRSTFGGSFVVDNYALLFKGLFLGSLLLVLLMSQRYVDAGTAYQG
                     EFYFLVLSSFVGMLLMPSSRDLLMLFLSLEIVSATGFIAAGFRKGDARGNEGALKFLL
                     IGVLSTAVMLFGMSLVYGFTGSLQLGPIAERLAQLGDEPAAVMSVFLVITGFAFKVSA
                     APFHFWAPDTYEGSPVPVAAYLSVASKAAGFAGLLQVTFVAFPSYARYWAPAFAVLAA
                     FTMLLGNVIALAQTNMVRLLAYSSVAQAGYMLVPFAAGASANPEVREEAFQAVLVYLL
                     IYAFTNLGAFAVVTLVSQRQPSNLIADYAGLARRAPALGAAMTLFLFSRAGIPPAAGW
                     YAKFVVFKSAINGDTLTLQLLVVFMAVMTVVALFYYAGVARSLWLGSPPEGAPEPALQ
                     PVAPGVGAAVALAAAGVLVLGVLPNLLVQYAPVSTLLAGP"
     gene            complement(2621..>3020)
                     /locus_tag="VFO47_10635"
     CDS             complement(2621..>3020)
                     /locus_tag="VFO47_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET8789193.1"
                     /translation="TLMPRLAGVFTLACIASLGLPGLAGFWGEILALLAAWNPAPGLS
                     VPLFRSLALVGLVGTLLTAGYFLWLLQRVNLGRVPDRWEGGTLGDIAPIEIAAWSPLI
                     LLTVALGFAPGLVLWLADPSVTGWFTELFA"
BASE COUNT          530 a         1000 c         1126 g          364 t
ORIGIN      
        1 gaccgaggcg gccgaggccg atccccgcct gggagtacgg gaacgtcgga tccttgccgc
       61 cgtgtatcgg gagcttgtcc accagcgaga gttggactag atgagtgatt ccaagggatg
      121 cgtgaggtga ggagggtgcc catgatcggc gttgctaccc aacgtcgacc tcgtgggagg
      181 cctcgtggac gccgacctgg acaccctcgc gacggcactg tacgtcaaga tcgacgagct
      241 gctgcagcaa gcaccgcagc tggcgccatg gcgacccacg gtcggcatcg cacccaagct
      301 cagcgacgcc gaactggtca ccttggccgt catgcaagcc ctgctcggct tcacctccga
      361 ggcccgctgg ctgcgtcacg ccgaccgcca cctgcgccat ctgttcccct acctgccccg
      421 gcaagctggc tacaacaagc gcctgcgggg cgcggctggg ctgctgcgcc acgccatccg
      481 ggccgtcgcc accgacacca gcctgtggac cgacgacatc tggatcgtcg actccacccc
      541 ggtggagtgc ggccgttccc gggagaccac caagcgttcg gcgctggccg gctgggccca
      601 atacggctac tgcgccagcc actcgcgcta cttctggggg ctgcggctgc acctggtctg
      661 caccctgcat ggcctcccgg tcgccttcgc gttgaccggc gccaaggccg acgagcgcca
      721 ggtgctggtg gacctgctgg cggtcgagcc ggaactggtg gccgcccggc ctgggcagac
      781 gctgctggcc gacaagcact actacggccg cgagttccag gcgatcctgg ccgggctggg
      841 cgtgcggctg ctgcggccgg cccgcaaggg cgagccccag cgggccggtg cccacctgtt
      901 ctgcccgctg cggcagctca tcgagtcggt caaccagacc ttcaaaggcc agctcgacct
      961 ggagcgccac ggtggccgta cctgtggcgg ggtcgcggtc cgggtcttgc agcgcatcct
     1021 cgccctggcc gtcgccatct ggcacaacca caagaccgga caaccgaccc tacggtccct
     1081 gatcgcctac gaccattgac cttggaatca atcgtctagc ggcgatggcg cctacggacc
     1141 cgcgagcagg gtcgagacgg gggcgtactg gaccaggagg ttggggagga cgccgaggac
     1201 caggacgccg gccgcggcca gggcgacggc ggcgccgacg cccggagcga cgggctggag
     1261 ggcgggttcg ggggcgccct cgggtgggct gccgagccag aggctgcggg cgaccccggc
     1321 gtagtagaac aaggcgacca cggtcatgac ggccatgaac acgaccagca gctgcagggt
     1381 cagggtgtcg ccgttgatgg ccgatttgaa gaccacgaac ttggcgtacc agccggcggc
     1441 cgggggaatg ccggcccgcg agaagaggaa gagggtcatg gccgccccga gggccggggc
     1501 gcggcgggcc aggccggcgt agtcggcgat caggttggac ggctggcgct ggctgaccag
     1561 ggtgacgacg gcgaacgcac ccaggttggt gaaggcgtag atcagcagat agacgagcac
     1621 ggcctggaag gcctcctcgc ggacctcggg gttggccgag gcgccggccg cgaacgggac
     1681 cagcatgtag ccggcctggg cgaccgagga gtaggcgagc agccggacca tgttggtctg
     1741 ggccagggcg atgacgttgc cgagcagcat ggtgaaggcg gccagcacgg cgaaggccgg
     1801 cgcccagtag cgggcgtagg acgggaaggc cacgaacgtc acctggagga ggccggcgaa
     1861 cccggccgcc ttggaggcga ccgacaggta ggcggccacc gggaccgggg agccctcgta
     1921 ggtgtcgggg gcccagaagt ggaacggggc cgccgacacc ttgaaggcga agccggtgat
     1981 gaccaggaac acgctcatca cggcggccgg ctcgtcgccc agctgggcca ggcgctcggc
     2041 gatggggccg agctggagcg agccggtgaa gccgtagacc aggctcatgc cgaacagcat
     2101 gaccgcggtc gacaggaccc cgatgagcag gaacttgagc gcgccctcgt tgccgcgggc
     2161 gtcgcccttg cggaagccgg cggcgatgaa gccggtggcc gagacgatct cgagcgacag
     2221 gaacagcatg aggagatctc gcgaggacgg catcagcagc atgccgacga agctggacag
     2281 gaccaggaag tagaactcgc cctggtaggc ggtgccggcg tcgacgtagc gctggctcat
     2341 cagcaggacc agcagcagcg agcccaggaa caggcccttg aacagcaggg cgtagttgtc
     2401 gaccacgaac gacccgccga aggtgctccg cgtgcccccg ccgaccaggc tggccagggc
     2461 gatcccggcg gccacggtgc cggcgatgga cagccagctg gcgaccacct tggcccgctc
     2521 cacgaacagg tcgaccacca gcaccagcag ggcggtgccg gccaggacca gctcgggggc
     2581 gatggcgtgg aagtcgatgg ccacggtcag ggtctcctcg tcatgcgaac agttccgtga
     2641 accagccggt gaccgacggg tcggccagcc acaggaccag gccgggggcg aacccgaggg
     2701 ccaccgtgag caggatcagg ggcgaccagg cggcgatctc gatgggggcg atgtcgccaa
     2761 gggtgccgcc ctcccagcgg tcggggaccc ggccgaggtt gacccgctgg aggagccaga
     2821 ggaagtagcc ggcggtcagc agcgtcccca ccaggccgac cagggccagg ctgcggaaca
     2881 gcggcaccga caggcccggg gccgggttcc aggcggccag cagggccagg atctcccccc
     2941 agaagccggc cagcccgggc aggccgaggg aggcgatgca ggccagggtg aacaccccgg
     3001 ccagccgggg catgagggtg
//
LOCUS       DASSWR010000636         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_927978,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000636 DASSWR010000000
VERSION     DASSWR010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927978"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..617)
                     /locus_tag="VFO47_10640"
                     /pseudo
     CDS             complement(1..617)
                     /locus_tag="VFO47_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(519..625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(781..1572)
                     /locus_tag="VFO47_10645"
     CDS             complement(781..1572)
                     /locus_tag="VFO47_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8789194.1"
                     /translation="MVGAVVAGAVGVGGLSAPHPPAGTGPAGRRSAGGAGADPANGCP
                     RRGGRHPGKSDPIDAEAVALAALRHPDLPVAELDGPTREVKLLVDYRRDLVRQRTRVA
                     NRLRWHLHELDPALQIPSRGLRRYKVIDELAVRLAGMEGTVARLARRLLARCRELTAE
                     INALEAELRPLVRQLAPTLLAVPGCGVLGAAMILGETAGVGRFRSKDAYARFTGTAPI
                     PVWSGDRRGKVRLNRGGNRTVNTALHMIAVTQVRGLDLAKRMSSG"
     gene            complement(1494..1724)
                     /locus_tag="VFO47_10650"
                     /pseudo
     CDS             complement(1494..1724)
                     /locus_tag="VFO47_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1913..2193)
                     /locus_tag="VFO47_10655"
                     /pseudo
     CDS             complement(1913..2193)
                     /locus_tag="VFO47_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          340 a          802 c          751 g          300 t
ORIGIN      
        1 ccgccctggc agccgtggtc gctgtggtgc accagaccag gggtgggatt gcgctgggtg
       61 atggccatgt ccagggcgtc cagggccagg tcggcgcgaa gcgaccgcga ggcctgccag
      121 cccacgatga accgggagaa cgcgtcgatg accaaggcca catagacaaa gccggaccag
      181 ctggcgacgt aggtcaggtc ggagacccac agctggttcg gcctgctcgc tgagaagtcc
      241 cgctccacca ggtcggcagg tcgggcggcg gccgcctcgg gcgtggtggt cttgtggggc
      301 ttgccgcggc ggacgccttc caggcccagt tcgcgcatga ggcgctcgac ggtgcagcga
      361 gccaccgcga tcccttcacg gtgcagctgc cgccacacct tgcgggcgcc gtagacgccg
      421 aactgctcgg cgtggacccg acggatctcg acctgcaagg cggcatcccg gaccgcccgc
      481 gctgatggcc gacgcttgcg atgctggtag taggtggacg gggcgatcgg cagcagctgg
      541 cagatcggct cgaccccgaa ccggtccttg tgacggtcga tgtaggcgat catctggtcg
      601 gtcggcggtc gagctccgcg atgctcctat gtcaagccgc ccgagcgctc gccgcggcgt
      661 ggctggtctg ggtgctggtg gagcgggcct gctcgtcggc gcgcagcgcg gtgaagacca
      721 cgtcagagag tcgccggcgc agaagccgga cagcctcggt gggggtcttg gcccacaaca
      781 tcagccgctc gacatacgct tggccaggtc cagcccgcgg acctgggtga ccgcgatcat
      841 gtgcagcgcg gtgttgaccg ttcggttgcc gccgcggttc agccgaacct tgccgcgccg
      901 gtctccggac cacactggga tcggggcggt cccggtaaag cgggcatagg catccttgga
      961 gcggaaccga ccgaccccgg cggtctcgcc caggatcatc gccgcgccca acacgccaca
     1021 gccgggcacg gccaacagcg tcggcgcgag ttgccgcacc agcggccgca gctcggcctc
     1081 caaggcgttg atctcggcgg tcagctcccg gcagcgggcc agcaaccggc gggccagccg
     1141 ggccaccgtc ccctccatgc cggccagccg gaccgccagc tcgtcgatca ccttgtagcg
     1201 gcgcagaccc cgcgagggga tctgcagggc cgggtcaagc tcgtgcaggt gccagcgcag
     1261 ccggttcgcc acccgggtcc gctggcggac caggtcacgg cggtagtcga ccagcagctt
     1321 gacctcccgg gtcggcccat ccagctcggc gaccggcagg tcggggtggc gcagcgcggc
     1381 cagcgcgacc gcctcggcgt cgatcgggtc cgacttgccg gggtggcggc cgccgcggcg
     1441 agggcagcca ttagccgggt cggcacccgc accacccgct gaccggcggc cagcaggtcc
     1501 tgttccagcc ggcgggtgag gtgccgacag tcctccaacg ccaaccgcac ctgcgaccac
     1561 tgcgccgacc accgcagcag ctccaggtgg ccatcgctgg tcgccctgac ggtccgctca
     1621 cccagccagc ggcccacctg gtcgaccgcc accatcgtat gggtgcgctt gtgcgagtcg
     1681 gccccgatga cgatcacggc atctccctgc gctcggctga ccatgagtca tccggcccgg
     1741 cagggcgggc acgcctcaat tgggcacgag gcaagctcct atcaagccac acacgctgct
     1801 gggccgaaca gcccggcggg gccgcacatc gctggacaag ccaccagcta cagccggggc
     1861 agcaaagaaa cgagcgaacc ccaccggacg ggcctcatcc tggcattgac ccgaagaaag
     1921 ccgccgcgga cttcaggatc tcattggccc gccgcaactc ccagacctcc cgctccagcg
     1981 tcttcagccg ctggcgcacg tcgctagtca gccccggccg cagcccctca tcggtctcgg
     2041 ccctccggac ccagttgcac agcgtctcgg ccgacacccc gaacttgtgg gcgagcgagc
     2101 agatcgccgc ccactgcgag gggtgctcgc cctggtgctc caacaccatg cgcgacgccc
     2161 gctcccgcaa ctcctgggga tacctgccgg gac
//
LOCUS       DASSWR010000637         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_729455,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000637 DASSWR010000000
VERSION     DASSWR010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729455"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFO47_10660"
     CDS             <1..1101
                     /locus_tag="VFO47_10660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET8789195.1"
                     /translation="LLVVFIALEILSLSLYVLAGQTRRLAAQEAAMKYFLLGAFSSAF
                     LLYGIALCYGATGTTNLLGIARASGDPTGDGTLLLAGVGLLAVGFCFKVSAVPFHMWT
                     PDVYQGTPTPVAAFMAAATKVAGFAVFLRVFAGALGGLVVTWRPAIAAIAVVTMLVGA
                     ALAVVQTDLKRMLAYSSISHAGYLLVGVAAAATVPAGVSASMFYLLVYAFMVMGAFMV
                     VQYVSRLGPADDEAIRERSSLEDYRGLARRHPWAAGLLAVFMLALTGIPPLSGFWAKY
                     YVFLAGIEAGLTWLVVVAVISSVISAFFYLRVLVVAYLQEPVGDAEAETSPGPTRALG
                     LALGLAGLLTVAIGLAPEALISAARSAERILG"
     gene            1104..>2038
                     /locus_tag="VFO47_10665"
     CDS             1104..>2038
                     /locus_tag="VFO47_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8789196.1"
                     /translation="MSRARSDATDDLAGIQADVKAGLGRVEEALGEAVTTADPFVHEA
                     ASYLVTAGGKRLRPTLVLIAGHTGDPAEPRLVGAAVAIELTHLSSLYHDDVMDEAELR
                     RGKRSANARFDNKVAVLTGDYLFARASEVTASLGTEATRVLARAIARLTQGQIREVRG
                     PGPDDDPVEHYLAVLADKTGALIAAASRLGGYLAGADPRVVGALTEFGERVGRAFQLG
                     DDLLDITADAGVAGKVPGSDLRAGVRTLPILYLLRDGGPDADRLTAVLDGDHDNGAVS
                     EALDLLRHSPALAEARRTAQAEVDAARAALEGVPAG"
BASE COUNT          233 a          776 c          727 g          302 t
ORIGIN      
        1 ctgctggtgg tgttcatcgc cctggagatc ctgtcgctca gcctctatgt cctggccggc
       61 cagacccgcc gcctggccgc tcaggaggcg gccatgaagt acttcctcct gggggcgttc
      121 tcctcggcgt tcctgctgta cgggattgcg ctctgctacg gggccaccgg caccaccaac
      181 ctgctcggca tcgcccgggc cagcggcgac ccgaccggcg acggcaccct gctgctggcc
      241 ggggtcggcc tgctggcggt cgggttctgc ttcaaggtgt cggcggtgcc gttccacatg
      301 tggacccccg acgtctacca gggcaccccg accccggtgg ccgcgttcat ggccgcggcc
      361 accaaggtgg ccggcttcgc cgtgttcctg cgggtgttcg ccggggccct cggcgggctg
      421 gtggtgacct ggcggccggc catcgccgcc atcgccgtgg tcaccatgct ggtcggggcg
      481 gccctggccg tggtccagac cgacctcaag cggatgctcg cctactcctc catcagccac
      541 gccggctacc tgctggtcgg ggtggccgcg gccgccaccg tgcccgctgg ggtgtcggcg
      601 tccatgttct acctgctggt ctacgccttc atggtgatgg gggcgttcat ggtcgtgcag
      661 tacgtgtcac ggctcggacc ggccgacgac gaggcgatcc gggagcggtc gtcgctggag
      721 gactaccggg ggctggcccg ccgccacccg tgggcggccg ggctgctggc cgtgttcatg
      781 ctggccctca ccgggatccc gccgctgtcg ggcttctggg ccaagtacta cgtgttcctg
      841 gccggcatcg aggctggcct gacctggctg gtggtggtgg ccgtgatcag ctcggtcatc
      901 tccgccttct tctacctgcg ggtgctggtc gtcgcctact tgcaggagcc ggtgggcgac
      961 gccgaggccg agacgtcacc cgggccgacc cgggccctgg ggctcgccct cggcctggcc
     1021 ggcctgctga ccgtggccat cggcctggcc cccgaggcgc tgatctcggc ggcgaggtcg
     1081 gccgagagga tcctggggta ggcgtgtcgc gggccagatc cgacgccacc gacgatctcg
     1141 ccggcatcca ggccgacgtc aaggccgggc tcggccgggt cgaggaggcg ctgggcgagg
     1201 cggtgaccac cgccgacccg ttcgtccacg aggcggccag ctacctggtc accgccggcg
     1261 gcaagcggct gcgccccacc ctggtcctga tcgccggcca caccggcgac ccggccgagc
     1321 cccgcctggt cggggcggcc gtggccatcg agctcaccca cctgtccagc ctctaccacg
     1381 acgacgtcat ggacgaggcc gagctgcgcc ggggcaagcg ctcggccaac gcccgcttcg
     1441 acaacaaggt ggccgtgctc accggcgact acctgttcgc cagggcctcg gaggtcacgg
     1501 ccagcctcgg caccgaggcc acccgggtgc tggccagggc gatcgcgcgg ctcacccagg
     1561 gccagatccg cgaggtccgc ggccccggcc ccgacgacga cccggtcgag cactacctgg
     1621 ccgtgctcgc cgacaagacc ggcgccctga tcgcggccgc cagccggctc ggcggctacc
     1681 tggccggggc cgacccgcgg gtggtgggcg ccctgaccga gttcggcgag cgggtcgggc
     1741 gggcgttcca gctcggcgac gacctgctcg acatcaccgc cgacgccggc gtcgccggca
     1801 aggtccccgg cagcgacctc cgggccgggg tccgcaccct gcccatcctc tatctgctgc
     1861 gcgacggcgg ccccgacgcc gacaggctca ccgcggtgct ggacggtgac cacgacaacg
     1921 gcgccgtcag cgaagccctc gacctgctgc gccactcacc ggccctggcc gaggcccgcc
     1981 gcaccgccca ggccgaggtc gacgccgccc gcgccgccct ggagggagtc cccgccgg
//
LOCUS       DASSWR010000638         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1210014, whole genome shotgun sequence.
ACCESSION   DASSWR010000638 DASSWR010000000
VERSION     DASSWR010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210014"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="rpsA"
                     /locus_tag="VFO47_10670"
     CDS             complement(<1..1152)
                     /gene="rpsA"
                     /locus_tag="VFO47_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET8789197.1"
                     /translation="MTESTERPTATEQDVSGVTTQVTQNDVGSAEDLAAAYEESLKAF
                     EEGDIVDGYVVKVDRDEVLLDIGYKSEGVIPSRELSIKHDVDPNEVVSLGDHIEALVM
                     QKEDKDGRLILSKKRAQYERAWGTIEKKKENDEIVEGTVIEVVKGGLILDIGLRGFLP
                     ASLVEMRRVRDLHPYVGRTLEAKIIELDKNRNNVVLSRRAWLEETQSATRRAFLHTLR
                     KGEVRKGVVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGQEVEVEVLDV
                     DLERERVSLSLKATQEDPWRQFARSHQIGELIPGRVTKLVPFGAFVRVDDGIEGLVHI
                     SELAERHVDIPEQVVSVGEEITVKVIDIDLDRRRISLSLKQAAQDQAQLT"
     gene            1355..2092
                     /locus_tag="VFO47_10675"
     CDS             1355..2092
                     /locus_tag="VFO47_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET8789198.1"
                     /translation="MTEPLYQHDAYLAAFDAQVTGVEPDGVVLDRSAFFPGGGGQPAD
                     IGILETEGVELAVTGAQRREGRVVLLVDGEPPPAGAKVRGRLDWQRRHALMRTHTALH
                     MLSGVIGRDYGALVTGGNFEEQKARMDFELSSMSAEFAEAVERALRVEVDADRPVHVS
                     FLSPEEFASRPELIRTKANLVPQGLDVVRVIDIEGLDRQADGGTHVASTGEVGTIYVV
                     GHQSKGKGNKRLRIALDPVPAPPSRSE"
     gene            2100..2732
                     /locus_tag="VFO47_10680"
     CDS             2100..2732
                     /locus_tag="VFO47_10680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8789199.1"
                     /translation="MTEASWVGWVARLQAIAQAGLELTDGPYDRERYAALRALATEIA
                     AAHLELPSPAERMALDELLAGDGGYPTPKVDVRALVRRDREVLLVRERADGRWSLPGG
                     WADVGWSPAAMVEREVAEESGFPVRARRLLARWDRSRHNPGLYPHSVYKVAVACDLLG
                     GEARPSIETLAAGWFPPGALPELSTGRITAAQIARLIELDDHPELPPDLD"
     gene            complement(2729..>3073)
                     /locus_tag="VFO47_10685"
     CDS             complement(2729..>3073)
                     /locus_tag="VFO47_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789200.1"
                     /translation="PGGGWGWDGRIGDRTTVRLDGAGTYPFFCYAHPGMVGVVVVGDG
                     QGTGSSVVEVADSGTPPGGAAPAGAASAGAAAAAEPAAARFPTAWLVLVAFCALVGAV
                     AGTRLAVRQPKG"
BASE COUNT          422 a         1085 c         1117 g          449 t
ORIGIN      
        1 ggtgagctgg gcctggtcct gggcggcctg cttgagcgac agggagatgc ggcggcggtc
       61 caggtcgatg tcgatcacct tgacggtgat ctcctcgccg acgctgacca cctgctcggg
      121 gatgtcgacg tggcgctcgg ccagctccga gatgtggacc aggccctcga tgccgtcgtc
      181 gacccggacg aaggcgccga agggcaccag cttggtcacc cggcccggga tcagctcgcc
      241 gatctggtgg gagcgggcga actggcgcca cgggtcctcc tgggtcgcct tgagcgacag
      301 cgagacccgc tcgcgctcca ggtcgacgtc caggacctcg acctcgacct cctggccgac
      361 ctcgaccacc tcggaggggt ggtcgatgtg cttccaggac agctcggaca cgtgcaccag
      421 gccgtccacg ccgcccaggt ccacgaaggc gccgaagttg acgatgctgg agacgacgcc
      481 cttgcggacc tcgcccttgc gcagggtgtg caggaacgcc cggcgggtgg ccgactgggt
      541 ctcctccagc caggcccggc gggacaggac cacgttgttg cggttcttgt cgagctcgat
      601 gatcttggcc tcgagggtgc gcccgacata ggggtgcagg tcgcgcaccc ggcgcatctc
      661 gaccagggag gcgggcagga agccgcgcag gccgatgtcg aggatcaggc cgcccttgac
      721 gacctcgatg acggtcccct cgacgatctc gtcgttctcc ttcttcttct cgatcgtgcc
      781 ccaggcccgc tcgtactggg cccgcttctt ggacaggatc aggcggccgt ccttgtcctc
      841 cttctgcatg accagggcct cgatgtggtc accgagactg acgacctcgt tggggtcgac
      901 gtcatgcttg atggacagct cgcgggaggg gatgacgccc tcggacttgt agccgatgtc
      961 gagcaggacc tcgtcgcggt cgaccttcac cacgtagccg tcgacgatgt caccttcctc
     1021 gaaggccttc agcgactcct cgtaggcggc cgcgaggtct tcggcggagc cgacatcgtt
     1081 ctgtgtcacc tgggtggtta ccccagagac gtcctgctcg gttgcggtgg gtcgctcggt
     1141 ggactcggtc aactgctcgt ctcctagact gtcggacaga aggctccccc tgcagacgtt
     1201 ttcgtggcgg tgaggcgacg cgatgagcgt cagaacacac tggcagaaaa gcccacagtg
     1261 gttcggcaga tggtagccgt cgcagcttcg tcgcgtcaac ggtccatgcg cgtatgacct
     1321 gcgagtttgt ggtccttcga gaaaggatgg tcaagtgacc gagccgctct accagcacga
     1381 cgcctacctt gccgccttcg atgcgcaggt caccggggtc gaacccgatg gggtcgtcct
     1441 cgaccggagc gcgttctttc ccggcggggg cgggcagccg gccgacatcg gcatcctcga
     1501 aaccgagggg gtcgagctgg ccgtgaccgg cgcccagcgc cgcgagggcc gggtcgtgct
     1561 gctggtcgac ggcgagccgc cgccggccgg ggccaaggtc cggggccggc tcgactggca
     1621 gcgccgccac gccctgatgc ggacccacac cgccctgcac atgctgtcgg gggtgatcgg
     1681 ccgcgactac ggggcgctgg tcaccggggg caacttcgag gagcagaagg cccggatgga
     1741 tttcgagctg tcctcgatga gcgccgagtt cgccgaggcg gtcgagcggg cgctgcgggt
     1801 cgaggtcgac gccgaccggc cggtgcacgt gtcgttcctg tcccccgagg agttcgcctc
     1861 gcggcccgag ctgatccgga ccaaggccaa cctggtcccc cagggcctgg acgtggtccg
     1921 ggtgatcgac atcgaggggc tggaccgcca ggccgacggc ggcacccatg tggcctcgac
     1981 cggcgaggtc ggcaccatct atgtggtcgg ccaccagtcc aagggcaagg gcaacaagcg
     2041 cctgcgcatc gccctcgacc cggtgccggc cccgccgtcc cgctcggagt gaccggcgag
     2101 tgaccgaggc gagctgggtc ggctgggtcg cccggctcca ggcgatcgcc caggcggggc
     2161 tggagctgac cgacgggccg tacgaccggg agcgctacgc ggccctgcgc gccctggcca
     2221 ccgagatcgc cgccgcccac ctggagctgc cgtcgccggc cgagcggatg gccctggacg
     2281 agctgctggc cggggacggc ggctacccga cgcccaaggt cgacgtgcgc gccctggtcc
     2341 ggcgggaccg ggaggtgctg ctggtgcggg agcgggccga cggccgctgg tcgctgccgg
     2401 gcggctgggc cgacgtcggc tggtcgccgg cggcgatggt cgagcgcgag gtcgccgagg
     2461 agtccggctt cccggtccgg gcccggcggc tgctggcccg gtgggaccgg tcacgccaca
     2521 acccggggct gtacccccac tcggtctaca aggtggcggt cgcctgcgac ctgctcggcg
     2581 gcgaggcccg gcccagcatc gagacgctgg ccgccggctg gttcccgccc ggcgccctgc
     2641 ccgagctgtc caccggccgc atcaccgccg cccagatcgc ccggctgatc gagctcgacg
     2701 accacccgga gctgccgccc gacctggact agcccttcgg ctggcggacg gccaggcggg
     2761 taccggcgac ggccccgacc agggcgcaga aggcgaccag gaccagccag gcggtcggga
     2821 accgggccgc ggccggctcg gccgcggcgg ctgccccggc cgacgcggcc ccggccggcg
     2881 cggccccgcc gggcggggtg ccggagtcgg cgacctccac caccgacgag cccgtgccct
     2941 ggccgtcgcc gacgaccacc accccgacca tgcccgggtg ggcgtagcag aagaaggggt
     3001 aggtgccggc gccgtccagg cggacggtgg tgcggtcccc gatccggcca tcccagcccc
     3061 acccgccccc ggg
//
LOCUS       DASSWR010000639         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_52522,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000639 DASSWR010000000
VERSION     DASSWR010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52522"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFO47_10690"
     CDS             <1..847
                     /locus_tag="VFO47_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET8789201.1"
                     /translation="AKLAVERTRNSISIMQQQLDGVRSQLTDARASIAARAAVAYVQG
                     PANDLASLLAAGDPTDALERAQLLDLLATHDADQVLAARAIERSAQARTNELATVERK
                     QAAILDQMAARKARIEQLVVQTEQTLAELRAAERRRAAAVNRPAATAPSAGSPASPPP
                     PKAVSGSVGAVIRYAYAQLGKPYQWGATGPGAFDCSGLTLMAWAQAGVSLPHSSRAQI
                     GIGRQVTRSELQPGDLIFRYSPISHVSLYVGNGQQISATHTGSTVKLQSAFQGEIVGF
                     SRPTG"
     gene            932..>2255
                     /locus_tag="VFO47_10695"
     CDS             932..>2255
                     /locus_tag="VFO47_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HET8789202.1"
                     /translation="MMLVAVGVLALGVAVGAALLWPHGEVSGPAAAGQQDPTRLVSAT
                     LTRVQPLECEEADPGVPTSTCIRVEARLADGKQVRFDTTDLTGNTFRAGQRVRLSVLE
                     REGQPPFYNIRDLERTRPMLILAAVFMLAVLAFGRWQGIRSLIALALSFAVIVGFVVP
                     AILRGHSPVAVALVGAMAIMLISLYLSHGIGRKTTAAVVGTGVALVLTAALTAGFVAA
                     TSLTGLASEDALNASFQAGGLSFRGLLLAGIIIGGLGVLDDVTMSQASLVFQLRRADS
                     TASFGKLVTGALAVGRDHIAATVNTLFLAYAGASLPLLVLFTTSGDSLGTVATAEAVS
                     VEVVRALCGSIGLIAAVPLTTLLAAALTGRGAEADQPLATERRPAGRSSYALRGRLAP
                     EQDQQVFDTVLGLHAPRLSHAAVGVNSTSLPPDELPAGASQAAVTLRQL"
BASE COUNT          321 a          825 c          752 g          357 t
ORIGIN      
        1 ggcgaagctg gcggttgagc ggacacgcaa ctccatcagc atcatgcaac agcagctgga
       61 cggcgttcgc agccagctga cggacgcccg ggccagcatc gccgcccgcg cagcggttgc
      121 ctatgtccag ggcccggcca acgacctcgc ctcgctgctc gccgccggtg atcccaccga
      181 tgccctggag cgggcacaac tgctcgatct gctggcgacg catgacgccg atcaggtcct
      241 ggccgcacgg gccatcgagc gttcggcgca ggcccggacg aacgagctgg ccacagtcga
      301 gcgcaagcag gcggcgatcc tcgatcagat ggcggcgcgg aaggccagga tcgagcagct
      361 ggtcgtccag accgagcaga ccctggcgga gctacgagcg gccgagcgcc gccgggccgc
      421 ggcagttaac cggccggcgg ccacggcgcc ttcagcgggc agccccgcct cccctcctcc
      481 acccaaggca gtcagcggca gcgtgggggc ggtgatccgc tatgcctacg cccagctcgg
      541 gaagccctac caatggggcg cgaccggccc tggcgccttt gactgctccg ggttgacctt
      601 gatggcgtgg gcgcaggcgg gcgtgtcgct gccccactcc agccgagccc agatcggcat
      661 cggccgtcag gtcacacgaa gcgagctgca acccggtgat ctgatcttcc ggtacagccc
      721 catctcccac gtgtcgctgt atgtgggcaa cggccagcag atctccgcca cccacaccgg
      781 cagtacggtg aagctgcagt cggccttcca gggcgaaatc gtcggtttca gccgaccgac
      841 tggctgaagc ccgccgcctg gcatccggtt gccacggccc agcatgtaag gagcgccaac
      901 caatatggat gcagcggttg gccgccaccg gatgatgctg gtggcggtcg gagtgttggc
      961 gctcggcgtt gccgtgggtg ctgccttgct ctggccccac ggtgaggtca gcgggccggc
     1021 tgcggccggc cagcaggatc caacccggct ggtcagcgcc accctcaccc gtgtccagcc
     1081 gctggagtgt gaagaggccg atccgggcgt gcccacctcc acctgcatca gggtcgaggc
     1141 gcgattggcc gatggcaagc aggtccgttt cgacaccacc gacctgaccg gcaacacctt
     1201 ccgggccggc cagcgggtca ggctgtcggt gctcgagcgg gaaggccagc cgcccttcta
     1261 caacatccgc gacctggagc ggacccgacc aatgctcata ctggctgcgg tgttcatgct
     1321 ggccgtattg gccttcggtc gctggcaggg aatccgctcc ctgatcgctc tggcgctgtc
     1381 gttcgccgtc atcgtgggat tcgttgttcc ggccatcctg cgcggccaca gccccgtggc
     1441 ggtggccctg gtcggggcga tggccatcat gctcatctcg ctgtacctga gccatgggat
     1501 cggccgcaag accaccgccg cggtggtggg caccggcgtt gccctggtat tgaccgccgc
     1561 cctgacggct gggtttgtcg ccgcgacctc cctcaccggc ctggccagcg aggacgccct
     1621 gaacgccagc ttccaggccg gtggcctgtc ctttcgcggg ctgctgctgg ccggcatcat
     1681 catcggcggc ctcggtgtgc tcgacgacgt gaccatgtcg caagcctcgc tggtgttcca
     1741 gctacgacgg gccgattcaa cggccagctt cggcaagctg gtcaccggtg ccctcgcggt
     1801 cggccgcgac cacatcgccg ccaccgtcaa caccctcttc ctcgcctatg ccggcgcgtc
     1861 gctgcccctg ctggttctgt tcaccaccag cggggactcg ctcggcaccg tggccaccgc
     1921 cgaggcggtg tccgtggagg tcgtccgggc cctctgcggg tcgatcgggc tcatcgcggc
     1981 ggtacccctg accacgctgc tggctgccgc cctcaccggg cggggcgcag aggccgacca
     2041 gccccttgcc accgagcgac gcccggccgg tcgcagttcc tatgcgctgc gcggccggct
     2101 cgccccggaa caggaccagc aggtgttcga caccgtgctc ggcctgcatg ccccgcggct
     2161 gtcgcatgcc gccgtcgggg tgaacagtac ctcgcttccc cccgacgagc tgccggccgg
     2221 cgccagccag gccgccgtca cactccggca gctgg
//
LOCUS       DASSWR010000640         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_137763,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000640 DASSWR010000000
VERSION     DASSWR010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137763"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFO47_10700"
     CDS             complement(<1..316)
                     /locus_tag="VFO47_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789203.1"
                     /translation="MRRLALLALACLLLAGCVRPGGADFAQAREPACRYQGRQGTALV
                     VLMAQAVPSASQLPCVELLPAGWSVSDIFVRNGRVRFALDSDRVGMHAVQVVLERFCS
                     IGP"
     gene            complement(313..2022)
                     /locus_tag="VFO47_10705"
     CDS             complement(313..2022)
                     /locus_tag="VFO47_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HET8789204.1"
                     /translation="MEPTAGRPPLGVGEPAVPSRPQRVSRAGRRRRPSGEAPPLPRTL
                     NRSGRLWLTGAGAVLVLWAGVVANERTAVRLLELDHAVVAWFAGMRTEVVTDVMMALH
                     ALGSRWTSRVLFWSTVAALIVLRRFRHLFVFLGATLAVTALTGLFSFLYVWPRPLGVE
                     ILGDWSGYANPSRPVAALTALLMGMLYSLAPAGRLRRLGKPVVAVLVVALSVALIYLA
                     VNSPSDVLIAVVLGVTVPLVAFRMLTPNEVFPVTYHRGSSAHLDVGGRRGQAIHRALE
                     DQLGVQVAEIKPFGLSGSAGSTPLRVKVKGDPETWLFAKLYAGSHLRSDRWYKLGRTL
                     LYGRLEDEKPFNTVRRLVQQEDYALRLMHGAGVRSPEPFGIVEITPEREYLVVTEFFD
                     DAKELGDLEEVDDGIIDDGLRIIRRLWDAGLAHRDIKPANLLVRDGRVHLIDVFFAEV
                     RPSPWRQAVDLANMMLVLATRSSPEQVYRRARLQFNDDEIAEAFAATSGITMPSQLRR
                     MMRVQGRDLHAEFLRLLPELPPPIAIQRWSIRRIGLMAGVAALTLLVLLMALGTFESA
                     GLL"
     gene            2162..>2832
                     /locus_tag="VFO47_10710"
     CDS             2162..>2832
                     /locus_tag="VFO47_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8789205.1"
                     /translation="MVLVLLIAGCDRAPDPPPPEDPRRPVIQLASFDFPESELLGELY
                     GQALRQHGFPVELVVQLGAREVVAPALQQGKVDMVPEYLGSALNFLNDRDRVATADPG
                     LTHARLEQVFGPRGVSVLAYAPAEDRNGFVVSGDLARRHGLEKLSDLAPLASRLVFGG
                     PPECPQRPLCLKGLQDLYKLHFARFEAMASRPTTALALDTGEIDVGMLDTTNPNLAKI
                     GSDLV"
BASE COUNT          432 a         1045 c         1020 g          335 t
ORIGIN      
        1 cggggccgat gctgcagaac cgctccagca ccacctgcac ggcgtgcatg ccgacccggt
       61 ccgagtccag cgcgaaccgc acccgcccgt tgcggacgaa gatgtcgctc acgctccagc
      121 cggccgggag cagctcgacg cagggcagct ggctggccga cggcaccgcc tgggccatca
      181 ggaccaccag ggccgtcccc tgccgaccct ggtagcggca ggccggctcg cgggcctggg
      241 cgaagtcggc gccgcccggc cggacgcagc cggccagcag caggcaggcc agagccagca
      301 gcgccaggcg cctcacagca gccccgccga ctcgaaggtg cccagggcca tgagcagcac
      361 cagcagggtg agcgcggcca ccccggccat cagcccgatc cgccggatgc tccagcgctg
      421 gatggcgatc gggggtggca gctcgggtag cagccggagg aactcggcgt ggaggtcgcg
      481 gccctggacg cgcatcatcc ggcgcagctg ggagggcatg gtgatgccgc tggtggcggc
      541 gaaggcctcg gcgatctcgt cgtcgttgaa ctgcagccgg gcccgccggt agacctgctc
      601 ggggctggag cgggtggcca ggaccagcat catgttggcc aggtcgaccg cctggcgcca
      661 ggggctgggc cggacctcgg cgaagaacac gtcgatcagg tggacccggc cgtcgcggac
      721 cagcaggttg gccggcttga tgtcgcggtg ggccaggccg gcgtcccaca gccggcggat
      781 gatccgcagc ccgtcgtcga tgatcccgtc gtccacctcc tccaggtcgc cgagctcctt
      841 ggcgtcgtcg aagaactcgg tgaccaccag gtactcgcgc tcgggggtga tctccacgat
      901 cccgaacggc tccgggctgc ggaccccggc gccgtgcatc agccgcaggg cgtagtcctc
      961 ctgctggacc agccggcgga cggtgttgaa cggcttctcg tcctccaggc ggccgtacag
     1021 cagcgtccgg cccagcttgt accagcggtc cgagcgcagg tggctgccgg cgtacagctt
     1081 ggcgaacagc caggtctccg ggtcgccctt gaccttgacc cgcagcgggg tggagccggc
     1141 cgaccccgac aggccgaacg gcttgatctc ggccacctgg acacccagct ggtcctccag
     1201 ggcccggtgg atggcctgac cgcggcggcc cccgacatcc agatgggccg agctgccccg
     1261 gtggtaggtg accgggaaca cctcgttggg cgtgagcatg cggaacgcca ccaaggggac
     1321 ggtgacgcca aggacgaccg cgatgaggac atcgctgggc gagttcacgg ccaggtagat
     1381 gagcgccacc gacaaggcca cgaccagcac ggccaccacc ggtttgccca gccggcgcag
     1441 ccgcccggcc ggggccagcg aatacagcat gcccatcagc agcgcggtca gggcggcgac
     1501 cgggcgcgag gggttggcgt agccgctcca gtcgccgagg atctcgacgc cgagcgggcg
     1561 cggccacacg tacaggaagg agaacaggcc ggtcagggcg gtcacggcca gggtggcgcc
     1621 gaggaacacg aacaggtgcc ggaagcggcg caggacgatc agggccgcca ccgtcgacca
     1681 gaacaggacc cggctggtcc agcgcgagcc gagcgcgtgc agggccatca tgacgtcggt
     1741 gaccacttcg gtgcgcatcc cggcgaacca tgcgaccacg gcgtggtcga gctccaggag
     1801 gcgcacggcc gtccgctcgt tggcgaccac cccggcccac agcacgagca cggcgccggc
     1861 gccggtcagc cacagccgcc cgctgcggtt cagggtccgc ggcagcggcg gggcctcgcc
     1921 cgagggtcgc cggcgccgcc cagcgcgcga cacccgctgg ggccggctgg gcaccgccgg
     1981 ctcgccaacg cccaggggtg gccgccccgc cgtcggttcc atgcacagga gggtaacctg
     2041 agagaacacc ctgcaacagg agaggggaat ggtctgcggc cggccacggc gtggagtcgg
     2101 cctccggccg acggccaaca gaccccgacc ggcgagctcg ccggtcgggc tggtcgggct
     2161 cgtggtcctc gtcctgctga tcgccggctg cgaccgggcg ccggacccac cgccgccgga
     2221 ggacccacgg cggcctgtca tccagctcgc ctccttcgac ttccccgaga gcgagctcct
     2281 cggcgagctg tacggccagg ccctgcgcca gcacggcttc ccggtcgagc tggtggtgca
     2341 gctgggcgcc cgcgaggtgg tcgcgcccgc cctccagcag ggcaaggtcg acatggtgcc
     2401 cgagtacctg gggtcggccc tgaacttcct caacgaccgc gaccgggtgg ccaccgccga
     2461 ccccggcctg acccacgccc gcctggagca ggtgttcggg ccccgcgggg tcagcgtgct
     2521 cgcctacgcc cccgccgagg accgcaacgg gttcgtggtc agcggcgacc tggcccgccg
     2581 gcacggcctg gagaagctca gcgacctggc cccgctggcc agccggctcg tgttcggcgg
     2641 gccgcccgag tgcccgcagc gccccctctg cctcaagggc ctccaggacc tctacaagct
     2701 ccacttcgcc cgcttcgagg cgatggcgtc gcggccgacc acggcgctgg ccctggacac
     2761 gggcgagatc gacgtcggca tgctcgacac caccaacccc aacctggcca agatcggcag
     2821 cgacctggtc ca
//
LOCUS       DASSWR010000641         7337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_815696,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000641 DASSWR010000000
VERSION     DASSWR010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815696"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..2425
                     /locus_tag="VFO47_10715"
     CDS             224..2425
                     /locus_tag="VFO47_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET8789206.1"
                     /translation="MTSQSVLTLEPKLQPHLGPHAQVPRTRLLDLLEASSRTPVAAIL
                     APPGYGKTTLLAQWAGHDPRAFGWLTIDRRDNDPAVLLRDLAAAFERIAPGRALLEGA
                     GAARPEAVDALLPQLGSALSTAELPSVVVLDDVHLLRNEDCLDAVTRLIDYLPEGSQL
                     AIASRGEPPLPLARLRAEGRVVEVGPEELAMDHGEASSLLEGADVDLATADVDELVRR
                     TEGWPVALHLAVRAATGRRRADRVVTLTGDDRFLADYLQFELLSRLPERLVTFLTRCS
                     VLERMSGPLCDAVLATTGSADLLESLARSNLLTTPLDRRRHWYRLHRLFRELLRSQLE
                     RSDPGLAAELTRRAAEWCAANDLPEPALEYAMAAGDADRAAGLVVDAAPAVHADGRLD
                     ELERWFGWFEANGEVEGRAGLAVLGAWISALTGHPVAAERWAGAARRASAGAGRSEGP
                     EALLRAALCRGGVEQMGTDAELALVLAPAGGAWQAAAQLLLGISHLLAGRQDEAERHL
                     ADAAEVGEDARVDAGMLALAERSILAMARGEWRQAEALAGQASAGAHGAWRERYGSGA
                     LLHAVTARLAIHRGDVAQARDDLARAERLRPLLTWALPHLAVQVRLEMARTYLVLTDA
                     VAARTMLWEVDDLLSRRPRLGLLRDQAAELHAKLDTMRMDAVGASALTTAEVRLLRLL
                     GTHYSFREIGVQLYLSQHTVKSQAMSIYRKFGVSSRSEAIRHARDLGLLVE"
     gene            2442..2816
                     /locus_tag="VFO47_10720"
     CDS             2442..2816
                     /locus_tag="VFO47_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8789207.1"
                     /translation="MAAHEEQLRRLALHDEAFIESVLAVNLNTSLGDDDEALRLSPKT
                     HALVRLAALLALGAAPVSYQWNVGAALDAGATTDEILGTMIAVAPISGIARVVQATPD
                     VALPIGYDLDVAFEELDPDPPA"
     gene            complement(2765..4750)
                     /locus_tag="VFO47_10725"
     CDS             complement(2765..4750)
                     /locus_tag="VFO47_10725"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HET8789208.1"
                     /translation="MRSPNAGIGGRTQQAGTLAAATIMPITFQPTLMPRSALDQALVT
                     GISTSLNYAFAALIQDSIEAAALRGAGRTGTDPATVDRRTWRRASVVLDLVAIGAGLA
                     FQSALRPRPDERLPRGGARTAAWWLSATGLSGAVVGVVQEVTARPSDTQDHSFPAATF
                     AGAAMAAINDYRRRRWERQFPGVGTDDDAPVSAAKAFAMSLVVTAALTGLAAGERLFA
                     AGVGRAAERVLPGHERLYRPLGHLAALSVLGGALYGMLRRVDHRIERGAEEIEAAYPI
                     PPTSPLVSGGPGSGVAWDTLSRQGRRNVATKVTADQIEQVMGEPALAEPIRVYIGLES
                     APTEAERVALAIAELERTGAFDRSLLMVISPTGTGYVNYVAVESAEYLTRGDMASVTM
                     QYSLRPSPLSLDRVAEGRHHYRMLIDAIHNALAERPPEKRPRVVLFGESLGAWTSQDA
                     FAHRGTQGLLNAGVDRALWIGTPYMSKWKEEVLHGDRPDVDRSLVGRFNDFGEVEALD
                     PEARRRLRYVMITHANDAVGHFGLDLLVRAPDWLGPAATRAATVPGAEQWRSPTTFVQ
                     TLIDMKNAANVIPGEFEAKGHDYRADLARFVREVYALPATDEQLAAVEQALRAAELER
                     KRLLDAAKAAAKAAVKDGDDGQAGGSGSSSSKATSRS"
     gene            complement(4902..5438)
                     /locus_tag="VFO47_10730"
     CDS             complement(4902..5438)
                     /locus_tag="VFO47_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789209.1"
                     /translation="MAIGPVQLLVLGFKHPDFTGEIREELDRLRDNDLIRVIDALGVR
                     KDADGNVQTLHASQLSGDEQAAFGALIGGLIGLGAAGEEGFELGAERGAEAVAERGGV
                     FDEEEAWDVVEEIPPDTAGLLVLLEHRWAIGLRDAIARAGGFRLASEFISPLDLVAIG
                     LVSAEEAEALAAADSGAV"
     gene            5599..5868
                     /locus_tag="VFO47_10735"
     CDS             5599..5868
                     /locus_tag="VFO47_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789210.1"
                     /translation="MDAEILAAEVAESLDEFLDHMSVAEGIGAKLNRQLLGFLKTEVL
                     PQANRAAELGVDPTPLFAMVIRVLRTYADSLERPPVADDRPDRPA"
     gene            complement(5865..>7337)
                     /locus_tag="VFO47_10740"
     CDS             complement(5865..>7337)
                     /locus_tag="VFO47_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET8789211.1"
                     /translation="QQAGRPAAGRRPAGLLSRLSEPTMRFLTRTAVLEQLSGPLCDAV
                     LGVSGSQRVLESLAGSNQLLVAVDHHRWYRYHQLLRELLLAELERREPELVPELHLRA
                     ADWYEGERLPELAVDHAQAAGDADRVARLVAALAVPAYAGGRVETARRWFRWFEDQDL
                     VERYPRVAVLGAQMEALLGDAPSAGRWAAAAERGQKAAPDRTLDASIALLRTFLCRDG
                     MAGMRADAATASAGLAPDDPWRATALLMEGIAHLLDGQAELADPILARVAGVAGPLGA
                     TPAASAALAERAIVALERRRWEEAGRLAEEAVAALRAGHLDDYAMAAFVRAVAARAAL
                     HLGDAPEAHQHLAQAVRLRPLLTGAMPHRSVQTLLELGRAYLALDDAAGARAVVRQAG
                     EILRERPGLGVLPEQAAELTATLERIGQGAVGASSLTAAELRLLPLLSTHLTLLEIGE
                     RLYLSRNTVKTQAVSIYRKLGVSSRSEAMQRVRELGLLGD"
BASE COUNT          888 a         2778 c         2729 g          942 t
ORIGIN      
        1 accgccgccg agctcgggct cccgccagcc aagatcctcg gggtcttcgg ggcgatcgcg
       61 ccgctcgtgg gcagcgcacg ggtcctgtcc gccgcctcca agctggtcgt ggccgggctc
      121 ctccccgccg aaccgcgtcg aatcacccca tctggatgat gatcgcggcc gggcgatcgc
      181 gcatggttgc tctggtgccg gcgagagaag cggggcggta gcgatgacca gccagtccgt
      241 cctgacgctc gagccgaagc tccagccgca tctcggaccc cacgcccagg ttccacggac
      301 ccgtctcctc gacctcctcg aagcctccag ccgcaccccg gtggccgcga tcctcgcccc
      361 cccgggctac gggaagacga ccctcctggc ccagtgggcc gggcatgacc cgcgggcgtt
      421 cgggtggctc acgatcgacc ggcgcgacaa cgaccccgcc gtcctgctcc gggacctggc
      481 cgccgccttc gagcggatcg cgccgggccg cgccctgctg gagggggccg gcgccgcccg
      541 gcccgaggcc gtcgacgccc tgctcccgca gctcgggtcg gccctgtcca cggccgagct
      601 gccgtccgtg gtcgtgctcg acgacgtcca cctgctacgg aacgaggact gccttgacgc
      661 cgtgacccgg ctcatcgact acctcccaga ggggtcgcag ctggccatcg ccagccgggg
      721 cgaaccgccc ctgccgctgg cccgcctgcg ggccgagggc cgggtggtgg aggttggccc
      781 cgaggagctg gccatggacc acggggaggc cagctccctg ctcgagggcg ccgacgtcga
      841 cctggccacg gccgacgtgg acgagctggt ccggcggacc gagggctggc cggtggcgct
      901 gcacctcgcg gtccgcgccg ccaccgggcg ccgccgcgcc gaccgggtgg tcaccctgac
      961 cggggacgac cggttcctgg ccgactacct ccagttcgag ctgctctccc gcctgccgga
     1021 gcggctggtc acgttcctga cccgctgctc ggtgctggag cgcatgtccg gccccctctg
     1081 cgacgccgtg ctggccacca ccgggtcggc cgacctgctc gagtccctgg cgcgatcgaa
     1141 cctcctgacc acgcccctgg accggcgccg ccactggtac cggctgcacc ggctgttccg
     1201 ggagctgctc cgcagccagc tcgagcgcag cgaccctggc ctggccgcgg agctgacgcg
     1261 gcgggcggcc gagtggtgcg ccgccaacga ccttcccgag ccggccctcg agtacgcgat
     1321 ggccgccggc gacgccgacc gggccgccgg cctggtcgtg gacgccgccc ctgccgtcca
     1381 tgccgacggg cgcctggacg agctggagcg gtggttcggt tggttcgagg ccaacggcga
     1441 ggtcgagggg cgcgccggcc tggccgtgct cggggcctgg atctccgccc tcaccgggca
     1501 cccggtcgcg gccgagcgct gggccggcgc ggccagacgg gcctcggccg gggccggtcg
     1561 gagcgaggga ccggaggcgc tgctccgcgc cgccctctgc cgcggcgggg tggagcagat
     1621 gggcaccgac gccgagctcg ccctggtgct ggcgccggcg ggcggcgcct ggcaggccgc
     1681 ggcccagctg ctcctcggga tctcgcacct gctggccggc cgccaggacg aggccgagcg
     1741 ccacctggcc gacgccgccg aggtcgggga ggacgccagg gtcgacgcgg gcatgctcgc
     1801 cctggccgag cggtcgatcc tggccatggc caggggggag tggcgccagg ccgaggccct
     1861 ggccgggcag gcctccgccg gcgcccacgg cgcctggcgg gagcggtacg ggagcggcgc
     1921 cctgctgcac gcggtgacgg cccggctcgc catccaccgc ggcgacgtcg cccaggccag
     1981 ggacgacctg gcccgggccg agcggctgcg cccgctgctg acctgggccc tgccccacct
     2041 ggccgtccag gtgcgcctgg agatggcccg gacctacctg gtcctcaccg acgcggtcgc
     2101 ggccaggacg atgctgtggg aggtggacga cctcctgtcg cggcggcccc ggctcgggct
     2161 gctgcgggac caggcggccg agctgcacgc caagctcgac accatgcgca tggacgccgt
     2221 cggggcgtcg gcgctgacca cggccgaggt gcggctcctg cggctgctgg ggacccacta
     2281 ctcgttccgg gagatcgggg tccagctgta cctgtcccag cacaccgtga agtcgcaagc
     2341 catgtccatc taccggaagt tcggcgtgtc gtcgcgcagc gaggccatcc gccacgcccg
     2401 cgacctgggc ctgctggtgg agtagggagg tgcgatggcc gatggcggca catgaggagc
     2461 agcttcgccg tctggcgctg cacgacgagg cgttcatcga gtccgtcctc gccgtcaacc
     2521 tcaacaccag cctgggcgac gacgacgagg cgctccggct gagccccaag acccacgccc
     2581 tggtccgcct ggccgccctg ctcgccctgg gggcggcacc cgtctcctac cagtggaacg
     2641 tcggcgccgc cctcgacgcc ggcgccacca ccgacgagat cctgggcacc atgatcgcgg
     2701 tggcgccgat cagcgggatc gcccgggtgg tgcaggcgac gcccgacgtc gcgctcccga
     2761 tcggctacga cctcgacgtc gccttcgagg agcttgaccc cgacccgccg gcctgaccgt
     2821 cgtcgccgtc cttgaccgct gccttggccg ccgccttggc cgcgtcgagc aggcgctttc
     2881 gttcgagctc ggcggcgcgc agggcctgct cgacggcggc gagctgctcg tcggtggccg
     2941 ggagcgcgta gacctcgcgg acgaagcggg ccaggtcggc gcggtagtcg tggcccttgg
     3001 cctcgaactc cccggggatg acgttggcgg cgttcttcat gtcgatcagc gtctggacaa
     3061 aggtggtcgg gctgcgccac tgctcggcgc cgggcacggt ggccgcgcgg gtggcggccg
     3121 gccccagcca gtcgggcgcc cggacgagca ggtcgagccc gaagtggccg acggcgtcgt
     3181 tggcgtgggt gatcatgacg tagcgcagcc ggcggcgggc ctcggggtcc agggcctcga
     3241 cctcgccgaa gtcgttgaac cggccgacca gggagcggtc gacgtcgggc cgatcgccgt
     3301 gaagcacctc ctccttccac ttgctcatgt agggggtgcc gatccagagg gcccgatcaa
     3361 cgccggcgtt cagcagcccc tgggtgccgc ggtgggcgaa ggcatcctgg ctggtccagg
     3421 cgcccaggct ctcgccgaac agcaccaccc ggggccgctt ctccgggggc cgctcggcca
     3481 gggcgttgtg gatggcgtcg atcagcatgc ggtagtggtg gcggccctcg gccacccggt
     3541 ccagcgacag cggggagggc cgcagcgagt actgcatggt caccgaggcc atgtcgccgc
     3601 gggtcaggta ctcggcgctc tccacggcca cgtagttgac gtacccggtg ccggtggggg
     3661 agatcaccat cagcagtgac cggtcgaagg cgccggtgcg ctccagctcg gcgatggcca
     3721 gggcgacccg ctcggcctcg gtgggggcgc tctccaggcc gatgtagacg cggatgggct
     3781 cggccagcgc cggctcgccc atgacctgtt cgatctggtc ggcggtgacc ttggtggcca
     3841 cgttgcgccg gccctggcgg ctgagggtgt cccaggccac gccgctgccg gggccgccgc
     3901 tgaccagcgg cgaggtcggc gggatggggt aggcggcctc gatctcctcg gccccccgct
     3961 cgatcctgtg gtcgacccgc cgcagcatcc cgtacagcgc ccccccgaga accgacaggg
     4021 cggcgaggtg gccgagtggc cggtagagcc gctcgtggcc gggcaggacc cgctcggccg
     4081 cacgcccgac gccggcggcg aacaggcgct cgccggcggc cagcccggtc agggcggcgg
     4141 tcaccacaag gctcatggcg aaggccttgg ccgccgacac cggcgcgtcg tcgtccgtcc
     4201 ccaccccagg gaactggcgc tcccagcgcc ggcgccggta gtcgttgatc gcggccatgg
     4261 ccgcgccggc gaacgtcgcc gccgggaacg agtggtcctg ggtgtcgctc gggcgcgcgg
     4321 tcacctcctg caccacgccg acgaccgcgc ccgacagccc ggtggccgac agccaccagg
     4381 cggccgtccg cgcccccccg cgcggcagcc gctcgtccgg ccggggccgc agggcgctct
     4441 ggaaagccag gccggcgccg atggcgacca ggtcgagcac gacgctggcg cgccgccagg
     4501 tccggcggtc gaccgtggcc gggtcggtcc cggtccggcc ggccccgcgc agcgccgccg
     4561 cctcgatgga gtcctggatg agggcggcga aggcgtagtt gaggctggtg ctgatgccgg
     4621 tgaccaacgc ctggtcgagc gcgctgcgcg gcatcagcgt cggctggaag gtgatgggca
     4681 tgatggtcgc cgccgccagc gtccctgcct gctgggtgcg gcccccgatg ccggcgttcg
     4741 gcgacctcat gcccatcccg ctcctcctcg ttgttcccgg ccggctcaga accggcggcg
     4801 gaccacgcgg cgggccgacc gccgccccgc gcggcgcccg ctgcggcgcg ccacccggcg
     4861 atttgccccg agcatatggc tcctcctttg tcctgtctgc ctcagacggc gccgctgtcg
     4921 gccgccgcca gcgcctcggc ctcctcggcc gacaccagcc cgatcgcgac caggtcaagc
     4981 gggctgatga actccgaggc caggcggaac ccgccggcgc gggcgatcgc gtcccgcagc
     5041 ccgatcgccc agcggtgctc cagcagcacc agcaggccgg cggtgtcggg agggatctcc
     5101 tcgacgacgt cccaggcctc ctcctcgtcg aacaccccgc cccgttcggc gacggcctcg
     5161 gcgccgcgct cggcgcccag ctcgaagcct tcctcgccgg cggccccgag gccgatcagc
     5221 ccgccgatca gggcgccgaa ggcggcctgc tcgtcgccgc tgagctggct cgcgtgcagg
     5281 gtctggacgt tgccgtcggc gtccttgcgg acgccaaggg cgtcgatcac ccggatcagg
     5341 tcgttgtctc ggagccggtc cagctcctcg cggatctcgc cggtgaagtc tgggtgcttg
     5401 aaccccagca ccagcagctg cacgggcccg atggccatgg tgtctcctcc ctgcgccgcg
     5461 gcgttccaag ggccacaggg tggcatcggg cctggaggcg ggcatcaccc gatcgggatg
     5521 agaatgggca ggtccggtta gccgcaagca cggaaggggt agcctccggt catgcctcct
     5581 ggtgacaccg gcccaccggt ggatgccgag atcctggccg cggaggttgc cgagagcctg
     5641 gacgagttcc tcgaccacat gtcggtggcc gaggggatcg gcgccaagct gaaccgccag
     5701 ctgctgggct tcctcaagac cgaggtcctg ccgcaggcca accgggccgc ggagctgggc
     5761 gtcgacccga cgccgctgtt cgccatggtc atccgggtgc tgcggaccta cgccgactcc
     5821 ctggagcggc cgcccgtcgc cgacgaccgg cccgaccggc cggcctagtc gccgagcaac
     5881 cccagctcgc ggacccgctg catcgcctcg ctgcgtgagg acacgccgag cttgcggtag
     5941 atcgagacgg cctgggtctt gaccgtgttg cgggagaggt acagccgctc gccgatctcg
     6001 agcagggtga ggtgggtcga gagcagcggc agcagccgca gctcggccgc ggtcagcgag
     6061 gacgccccga ccgctccctg cccgatccgt tccagcgtcg ccgtcagctc ggccgcctgc
     6121 tcgggcagga cgcccaggcc gggtcgttcc cggaggatct cgccggcctg gcgcacgacc
     6181 gccctggccc cggcggcgtc gtcaagggcc agataggcgc ggcccagctc cagcagggtc
     6241 tgcaccgagc ggtggggcat ggccccggtg agcagcgggc gcaggcggac ggcctgggcc
     6301 agatgttggt gggcctcggg ggcgtcgccc aggtgcagcg ccgcccgcgc ggccaccgcg
     6361 cgcacgaagg cggccatggc gtagtcgtcc aggtggccgg cccgcagggc ggcgacggcc
     6421 tcctcggcga ggcggcccgc ctcctcccag cggcggcgct ccagggcgac gatggcccgc
     6481 tcggccaggg cggccgaggc cgcgggcgtc gccccgagcg gcccggcgac gccggccacc
     6541 cgggccagga tcgggtcggc cagctcggcc tggccgtcga gcaggtgggc gatcccctcc
     6601 atcagcagcg ccgtcgcccg ccaggggtca tccggcgcca gcccggccga ggcggtggcg
     6661 gcgtcggccc gcatgccggc catgccgtcg cggcacagga aggtccgtag cagcgcgatc
     6721 gaggcgtcca gggtacggtc cggcgccgcc ttctgcccgc gctcggccgc ggccgcccag
     6781 cgtccggcgc tcggggcgtc gccgagcagc gcctccatct gcgcgcccag gaccgcgacc
     6841 cgcgggtagc gctcgaccag gtcctggtcc tcgaaccacc ggaaccaccg ccgggcggtc
     6901 tcgacccgcc cgccggcata ggccgggacg gccagggcgg cgaccagccg ggcgacccgg
     6961 tcggcgtccc cagcggcctg ggcgtggtcg acggccagct ccggcagccg ttcgccctcg
     7021 taccagtcgg cggcccgcag gtggagctcg gggaccagct cgggctcgcg gcgctccagc
     7081 tcggccagga gcagctcccg gagcagctgg tggtagcggt accagcggtg gtggtccacg
     7141 gccaccagca gctggttcga gccggccagc gactccagca cccgctggga cccgctgaca
     7201 ccgaggaccg cgtcgcacag gggcccgctc agctgctcca gcacggccgt gcgggtgagg
     7261 aagcgcatgg tcggctccga gaggcgcgac agcaggccgg ccggccggcg gccggccgcc
     7321 ggccggccgg cctgctg
//
LOCUS       DASSWR010000642         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1183373, whole genome shotgun sequence.
ACCESSION   DASSWR010000642 DASSWR010000000
VERSION     DASSWR010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183373"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFO47_10745"
     CDS             complement(<1..734)
                     /locus_tag="VFO47_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET8789212.1"
                     /translation="MAVNAEAAGRSRTGSARVAVQKFGTFLSGMIMPNIAAFIAWGFI
                     TAFFIETGWTPVGGIGGFGNDANGDPNIGLVGPMIRFMLPLLIAIQGGRMVYGIRGSV
                     VAAVATMGVIVSTPDPNFLGALIMGPLAAWCMKHVDKLWEGKIKPGFEMLVNNFSAGI
                     LAAALAVAGFFIFGPPIEAIATALGDGVDWLVDNSLLPLASLIIEPAKVLFLNNAINH
                     GVLTPLGIQQAAEEGKSILFLLEANPG"
     gene            complement(974..1924)
                     /locus_tag="VFO47_10750"
     CDS             complement(974..1924)
                     /locus_tag="VFO47_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8789213.1"
                     /translation="MEFRQLRYFIAVAEELHFGRAAKRLQISRPPLSQQILALERELG
                     VELLIRGRSIELTEAGRVLLEHGRSASEAVTYAVQAAREAGEGMTRLRVGYPAGAASA
                     SVPLAVRIFRERFPSVALDTVVGQAGAHLAALAARQLDVAFVRLAGANGDNLAFRPLH
                     REPVHVAIPELHPLARLRAVRAGHLANEPIVLFPRALDPPLHDHLVHDVLAGCGVAPS
                     VVLEATTLESSLVAVAGGLGIAFAAESAAGQFTVRGVEFRPLVTVAPPLQFGVSWRRD
                     TISRAVRQFLAVVFELAWVTRPATRNDAAMSGSLPHVGVG"
     gene            2089..3042
                     /locus_tag="VFO47_10755"
     CDS             2089..3042
                     /locus_tag="VFO47_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HET8789214.1"
                     /translation="MRGPVSYYDPDEVEPMVTAAVMYYQAHRSQAQIARHLGVSRPTV
                     SRLLARARQLGIVRIEIVPPTADPGLGKNLQERLGLRSIHIAAGLADPDDPAPVLAGR
                     LNDALAGIGLHPGDVLVVGWGRAIYSLGRYELAPLPGVVVVPALGGGAEDRPWFQANE
                     ITRRWAVTLHGTPRYLHAPAFVSSALKRSLVHEEGVHSTLDLWDRAKVALVGIGAYPK
                     PDSSLVAAGFSDGDPAIADATGDVVGRFFTDDGTLIHYPDEPRLLAITPDRMRRIPHV
                     VGISVGADKARAIVGAARARLVNTLVTDAGTAREVAALLDA"
     gene            complement(3060..3341)
                     /locus_tag="VFO47_10760"
     CDS             complement(3060..3341)
                     /locus_tag="VFO47_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789215.1"
                     /translation="MASRRRRPSASPGLVAFAVEAVALGGPVSLVDDAGRIASELVAS
                     TTRHTEAPVELTVDVGDDMVRIEVRDDDASLPGSGRVVSFELREASAAV"
BASE COUNT          536 a         1280 c         1247 g          493 t
ORIGIN      
        1 cccgggttgg cctccagcag gaacaggatc gacttgccct cctcggcggc ctgctggatg
       61 ccgagcgggg tcaggacgcc gtggttgatg gcgttgttca ggaacagcac cttggcgggc
      121 tcgatgatga ggctggccag cgggagcagg ctgttgtcga ccagccagtc gacgccgtca
      181 ccgagtgccg tcgcgatggc ctcgatgggc ggcccgaaga tgaagaaccc ggcgacggcc
      241 agcgccgcgg ccaggatgcc ggccgagaag ttgttgacca gcatctcgaa gccgggcttg
      301 atcttgcctt cccacagctt gtcgacgtgc ttcatacacc aggcggcgag cggccccatg
      361 atcagggcgc cgaggaagtt cggatctggg gtgctgacga tcacgcccat ggtggccacc
      421 gccgccacca cgctgccgcg gatgccgtag accatccggc cgccctggat ggcgatgagc
      481 agcggcagca tgaaccggat catcgggccg accaggccga tgttggggtc gccgttggcg
      541 tcgttcccga agccgccgat cccgccgacc ggggtccagc ccgtctcgat gaagaacgcg
      601 gtgatgaagc cccaggcgat gaaggcggcg atgttcggca tgatcatgcc ggacaggaag
      661 gtcccgaact tctgcaccgc gacccgggcg ctgccggtcc tgctgcggcc tgctgcttcg
      721 gcgtttacgg ccatgctgtg cctccgagcc gggcggcgcg cacgcctggc gacgtgcatc
      781 acgccgcgct ccgtcgcgac aggctgcctg ccgcggtaat cgtagggttg cctgcgcttc
      841 cgggttcgcg tcaggcccga tcgcctatgc tgccgccgac atcgccatcg cgtgcccttt
      901 ctcgcatccc tggagtggaa gccaccgcag cacgaacaac gatattgccc aggtcaggcc
      961 gagcgcccga ctgctaacca actccgacat gcggtaggga acccgacatg gccgcatcgt
     1021 tgcgggtggc cggcctggtc acccaggcca gctcgaacac cacggcgagg aactgccgga
     1081 cggccctcga gatggtgtca cgccgccagc tcacgccgaa ctgcagcggc ggggccaccg
     1141 taaccagggg gcggaactcg accccgcgga cggtgaactg tccggccgcc gactcggccg
     1201 cgaaagcgat ccccagcccg ccggcgaccg cgaccaggct gctctccagc gtggtcgcct
     1261 cgagtacgac cgacggcgcg acaccgcagc cggccaggac gtcgtgcacc aggtggtcgt
     1321 ggagcggcgg gtcgagcgcc cgcgggaaca ggacgatcgg ctcgttggcg agatgcccag
     1381 cccggacggc gcgcagcctg gccagcgggt gcagctcggg gatggccacg tggacgggct
     1441 cgcggtgcag gggccggaag gccaggttgt cgccgttggc gccggccagg cgcacgaagg
     1501 ccacgtccag ctgacgggcg gccagggccg cgaggtgcgc gccggcctgc ccgaccaccg
     1561 tatcgagggc gactgagggg aaccgctcgc ggaagatccg gacggccagc ggcaccgacg
     1621 ccgaggcagc cccggccgga taccctaccc gcagcctggt catgccttcg ccggcctccc
     1681 gcgccgcctg gacggcgtag gtgaccgcct cgctggcgct gcggccgtgc tcgagcagga
     1741 cccgtcccgc ctcggtcagc tcgatgctgc ggccgcggat cagcaactcg accccgagct
     1801 cgcgctccag ggcgaggatc tgctggctga gcggcggccg gctgatctgg aggcgcttgg
     1861 ccgcccggcc gaagtgcagc tcctcggcca cggcgatgaa gtagcgaagt tgccggaact
     1921 ccatccacgg ctcctcatgc ttgccgactt gaacgaagcc caaccttaga tgggaggtga
     1981 acaaatgtca agaacaaatg tgcaacatct gttcagagca cttgtgcatc ggccgggatg
     2041 gcgctatggt gaagctgcct cagcggaaca gtcgtgaggg gggacgccgt gcgcggaccg
     2101 gtcagctact acgatcccga cgaggtcgag ccgatggtca cggcggcggt catgtactac
     2161 caggcgcacc ggtcccaggc gcagatcgcc cggcacctcg gcgtcagccg cccgaccgtg
     2221 agccggctgc tggcccgggc ccgccagctc ggcatcgtcc ggatcgagat cgtccccccg
     2281 accgccgacc cggggctggg caagaacctc caggagcggc tcgggctccg cagcattcac
     2341 atcgccgccg ggctggccga ccccgacgac cccgcgccgg tgctagctgg ccggctcaac
     2401 gacgcgctcg ccggcatcgg cctccacccc ggtgacgtac tcgtcgtcgg ttggggccgg
     2461 gccatctaca gcctgggccg ctacgagctc gcccccctgc ccggggtggt ggtcgtgccg
     2521 gcgctgggcg gcggcgcaga agaccggccc tggttccagg cgaacgagat caccaggcgc
     2581 tgggcagtga ccctccacgg caccccccgc tacctccacg ccccggcgtt cgtctcgtcg
     2641 gcgctcaagc ggtccctggt ccacgaggag ggcgtccact ccaccctcga cctctgggac
     2701 cgggccaagg tcgccctggt gggcatcggc gcctacccca agcccgactc cagcctcgtc
     2761 gccgccggct tctccgacgg cgaccccgcg atcgctgacg ccacggggga cgtggtcggc
     2821 cgcttcttca ccgacgatgg gacgctgatc cactacccgg acgagccgcg gctgctggcc
     2881 atcacccccg accggatgcg gcgcatcccg cacgtggtcg ggatctccgt cggtgccgac
     2941 aaggccaggg cgatcgtcgg ggcggcccgg gcccggctgg tcaacaccct ggtcaccgac
     3001 gccgggacgg cccgcgaggt ggccgccctg ctcgacgcat aggcgcggcg ccggcccggt
     3061 cagacggcgg ccgaggcctc gcgcagctcg aaggagacga ccctgccgct gccggggagg
     3121 ctcgcgtcgt cgtcgcggac ctcgatgcgg accatgtcgt cgccgacgtc gacggtcagc
     3181 tcgaccgggg cctcggtgtg ccgggtggtg ctggccacca gctcgctggc tatccggccg
     3241 gcgtcgtcga ccaggctgac cgggccgccc agggccaccg cctcgacggc gaacgcgacc
     3301 aggccggggc tcgcgctcgg gcgccggcgt cttgatgcca tgaaggagcg gggcctccgc
     3361 tccccgtgac ggtacccggg tgccggacac tcgggccgtt cacaccagcc acgacgaggg
     3421 aggccccatg ggtgaacagt acgtcgggat cgacctgcac cgccgccgtt cggtgatcgt
     3481 ccgcatgacc cctgggggcg aggtgctaca gaccatccgt gtcgacaacg acccggtcgc
     3541 gctgtcgctg gagctc
//
LOCUS       DASSWR010000643         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_391919,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000643 DASSWR010000000
VERSION     DASSWR010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391919"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..673
                     /locus_tag="VFO47_10765"
     CDS             116..673
                     /locus_tag="VFO47_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET8789216.1"
                     /translation="MSTNRRKQQARQRAVRERAARQARRERAAELFAQGRTQAEVARE
                     LDVSGQSASRWHSRWRADGTAALRTRGPTGRQPKVADDQLEGIEQALLEGALAHGFAT
                     DVWTLDRIAMVIQGLTGVGLSNPSVWRLLRGRLGWTVQRPQRQAKERDEEAIQHWVAH
                     EWPRIKKGPAQNRPGSSSSTSRASR"
     gene            598..1191
                     /locus_tag="VFO47_10770"
     CDS             598..1191
                     /locus_tag="VFO47_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8789217.1"
                     /translation="MAPDQKRARAESAWIVFFDESGISLIPPVRRTWSRRGRTPILRH
                     RMAWKRASMAAALGYHPEGTSARLCFHLQADSYDTDSLIGVLEQLAAFYTGHRVVLLW
                     DGLSSHWSHKMRAHLAAQRHWLTVERLPAYAPELNPVEYLWANLKGGALANCTGDTIA
                     EVADATEQGIQRICDSDSLVVGFLAHTGLSLDTEPSP"
     gene            complement(1231..2059)
                     /locus_tag="VFO47_10775"
     CDS             complement(join(1231..1720,1719..2059))
                     /locus_tag="VFO47_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912324.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET8789218.1"
                     /translation="MADRLLPDALWQRLLPLLPPPPSRARGGAPRTVSDRACMAAVLF
                     MARTSTPWALLPVGEFGCGSVTTCWRRFAEWARAGVFERLQELLLDELGAAGALDWSR
                     VTVDSFSLRAVRGGDLTGANPVDRARPGSKLHLAAEGTGLPLSLLVTAANTPDAAVFQ
                     ALLDDIPRVRTPVGGRRCRPEKVHADKAYDQRRCRGYLTQRGIKVRIARRGIESSQRL
                     GRHRWKAERSIAWLAGCRRLRIRYDRDSGRFFAFAMLGCARLGYNRLPDTAEAHARSP
                     "
BASE COUNT          388 a          751 c          780 g          317 t
ORIGIN      
        1 agaggctatc aagcaagaaa agcttggtag attgcatata tgtcagtggt agtccttatt
       61 tgattgtttc gctttgaggt gacgatgggg tggctggtgt ggaagcatgc ccgctatgtc
      121 gaccaaccgc cgcaagcaac aagcacgaca gcgggccgtg agagagcggg cggcccgcca
      181 ggcccgtcgg gaacgggccg ctgagttgtt cgcccagggc cgtacccagg ccgaggtggc
      241 ccgtgagctg gacgtctcgg gccagagcgc cagccgctgg catagccgct ggcgagccga
      301 cggcacggcc gcgttgcgga ctcgtgggcc gaccggccga cagcccaagg tcgccgacga
      361 ccagctggag gggatcgagc aggcgctgct ggagggcgcg ctcgcccacg gcttcgccac
      421 cgacgtgtgg acccttgacc ggatcgccat ggtgatccag ggcctgaccg gggtggggtt
      481 gtcgaaccca tcggtgtggc ggctattgcg agggcggctg ggctggactg tgcagcggcc
      541 ccagcggcag gcgaaggagc gcgacgagga agcgatccag cactgggtgg cccatgagtg
      601 gccccggatc aaaaaagggc ccgcgcagaa tcggcctgga tcgtcttctt cgacgagtcg
      661 ggcatctcgc tgatcccacc ggtccggcgg acctggtcgc ggcgcggcag gacgccgatc
      721 ctgcgccacc gcatggcctg gaagcgggcc tccatggccg ccgcgctcgg ctaccaccca
      781 gaaggtacca gtgcgaggtt gtgcttccac ctccaggctg acagctacga caccgacagc
      841 ctcatcggcg tcctggagca gctcgcagcg ttctacaccg gccaccgggt ggtactgctg
      901 tgggacgggc tgagttcgca ttggagccac aagatgcgcg cccacctggc cgcccagcgc
      961 cactggctca ccgtcgagcg gctccccgcc tacgcccccg agctgaatcc agtcgagtat
     1021 ttgtgggcca acctcaaggg cggagcgctg gccaactgca ccggcgacac catcgccgag
     1081 gtcgccgacg ccaccgaaca aggcatccag cgcatctgcg acagtgacag cctggtggtc
     1141 gggttcctcg cccacaccgg cctgtccctc gatactgagc cgtcacccta acgtgaaagg
     1201 atcaaataaa aggacattcc ggaacctcga tcagggtgac cgtgcgtgtg cctcggcggt
     1261 atcgggaagc cggttgtagc ccagccgtgc gcagcccagc atggcgaagg cgaagaaccg
     1321 cccggagtcc cggtcatagc ggatccgcag ccgccgacac ccagccagcc aggcgatcga
     1381 gcgttcggcc ttccagcggt ggcgacccag ccgctgcgac gactcgatcc cacggcgggc
     1441 gatgcggacc ttgatccccc gctgggtaag gtagccgcgg cagcggcgct gatcgtaggc
     1501 cttgtcggcg tgaaccttct cggggcggca gcgccgaccg ccgacagggg tccgcaccct
     1561 ggggatgtcg tccagcagcg cctggaacac tgccgcatcg ggggtgttgg ccgcggttac
     1621 cagcagcgac agcggcagcc cggtgccctc agcggccagg tggagcttgg acccaggcct
     1681 ggcccggtcg accgggtttg cgcccgtcag gtcccccccg aaccgcgcgc aggctgaagg
     1741 agtccacggt gacgcgcgac cagtccagcg ctccggctgc gccgagctcg tccagcagca
     1801 gctcctggag ccgctcgaag accccggcgc gggcccattc agcgaaccgc cgccagcagg
     1861 tcgtcacgct gccgcagccg aactcgccga ccgggagcag cgcccagggg gtggaggtgc
     1921 gcgccatgaa caggaccgcg gccatgcagg cccggtccga gacggttcgg ggtgcgccgc
     1981 cacgcgcccg ggagggtggg ggtggcaaca gcggcaggag gcgctgccac agcgcatcgg
     2041 gcagcagccg gtcggccaga gttgtcatgg cccagatgat tgcccaccac cgacccaacc
     2101 cgctcctgcc gggccaagta gggcacttgc gaccgggtgg gttaccttcc aagacatggt
     2161 gagcaggagc agaacgatcg ccaggtgata cacctgacca agacggtgac ctggtggcac
     2221 gtcgtcgccg gcgttc
//
LOCUS       DASSWR010000644         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_391950,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000644 DASSWR010000000
VERSION     DASSWR010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391950"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1527
                     /locus_tag="VFO47_10780"
     CDS             205..1527
                     /locus_tag="VFO47_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET8789219.1"
                     /translation="MCWSRGGSERGSVAVTVALAMTVLLGFAALVVDIGLNWAARTSA
                     QTAADSAALAGASRLVADGGAAALLTVEQGLTDNGITLPGAVNWATDGNEGNGEVVCW
                     TLPAGAPGPGANCPDGSNALQVTTPAIEVQYAFAPLLGEATRSIKARAAAAAGPAAPN
                     NCVLCVLDEDDAAALASTAVGGIDVDGGGIVVNSDDAAALLLSGVGDVSADQIRVVGG
                     VDLSGGGALLPPAEEGGPPVPDPLADLLTPVELPFAPTPDLTPVRTIDVDTTLTPGVY
                     ERITVNGGATLTLEEGVYVVTDFDPVHTGFRVLDGGRVEGDGVTIYLACGDYPAPCDG
                     DAGTRFRLDPLSEFDVSPPASGGEYAGLSVFADPGNTRSQQLRGAVTLDGAVYAASAR
                     LAVFPTANVQVNSLVVVDRLTAASPFPLGVDYDPSLDIFGYGVPVLIS"
     gene            complement(1673..>2193)
                     /locus_tag="VFO47_10785"
     CDS             complement(1673..>2193)
                     /locus_tag="VFO47_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET8789220.1"
                     /translation="LVAERLGDSFVGWRRLAAAHAAGDDDLAGLDPWAARHLRRLADL
                     EAGWPQAARGPTLLHADLRADNLLLTPTRVVAVDWPWACVGAAWVDLLLLLPSVAMQG
                     GPDPEPTFTAHPVAAGADPSAVTTTLTAWAGFLIAGARLPPPPGLPTLRAFQHGQALV
                     ALDWLRRRTGWS"
BASE COUNT          273 a          763 c          869 g          288 t
ORIGIN      
        1 cgttcggccc ggcgatgtag ccgtccagtg aggtcgtgat gcccgcggcc accttcgtca
       61 tcgggtcctc ctttggggac gtccggctcg gacccggaca ggatcgccga cgcgagtgac
      121 agtcgcacct tgaccttgcg cgggttgtga gcagttcgtc gtgaactggc tgacgacacc
      181 ccgatcgggg gacgatctgc cgctatgtgt tggagtcggg ggggttcgga gcgggggagc
      241 gtcgcggtca cggtggcgct ggccatgacg gtgctgctcg ggttcgcggc cctggtggtc
      301 gatatcgggt tgaactgggc ggcgcggacc tcggcgcaga cggcggcgga ctcggccgcc
      361 ctcgccgggg cgtctcggct ggtggcggat gggggggcgg cggccctgct caccgtggag
      421 caggggctga ccgacaacgg catcaccttg ccgggggccg tgaattgggc gaccgacggg
      481 aacgagggca acggcgaggt cgtctgctgg accctccccg ccggcgcgcc cggccccggg
      541 gccaactgcc ccgacgggtc gaacgcgctg caggtgacca cgccggcgat cgaggtccag
      601 tacgcgttcg cgccgctgct gggcgaggcc accaggagca tcaaggcgcg ggcggcggcc
      661 gcggccgggc cggcggcccc caacaactgc gtgctctgcg tgctcgatga ggacgacgcc
      721 gccgccctgg cgtcgaccgc cgtgggcggg atcgatgtcg acggcggcgg gatcgtggtg
      781 aactccgacg acgccgccgc gctgctcctg tccggggtgg gcgacgtcag cgccgaccag
      841 atccgggtgg tcgggggggt cgacctgtcc ggcggcgggg cgctgctccc gccggccgag
      901 gagggggggc caccggttcc cgacccgctg gccgacctgc tgaccccggt cgagctgccc
      961 ttcgcgccga ccccggactt gaccccggtg cggaccatcg acgtggacac gaccctcacg
     1021 ccgggggtgt acgagcgcat caccgtgaac ggcggtgcca ccctgaccct cgaggaaggg
     1081 gtgtacgtgg tcacggactt cgaccccgtc cacaccgggt tcagggtgct ggacggcggc
     1141 cgggtggagg gtgacggcgt caccatctac ctggcctgcg gggactaccc ggcgccgtgc
     1201 gacggcgacg ccgggacccg gttccggctc gaccccctga gcgagttcga tgtcagcccg
     1261 ccggccagcg ggggggagta tgcggggttg tcggtcttcg ccgaccccgg caacacccgg
     1321 tcgcagcagc tccgcggcgc cgtcaccctg gacggggcgg tgtacgcggc cagcgcccgc
     1381 ctggccgtct tcccgaccgc caacgtccag gtcaactccc tggtggtcgt cgaccgcctc
     1441 accgccgcca gcccgttccc gctgggggtc gactacgacc ccagcctcga catcttcggg
     1501 tacggggttc ccgtcctgat cagctagatc gcggcgcctg ccggtacgtg agcggttcgt
     1561 gaggaccggg tggttcgatg ggcgccgagc ttgcggcagg cgctgagggg ccggcacccc
     1621 ggctggagaa gttgggccgg cccctcatcc aattcccggg atccccggca ggtcaggacc
     1681 agccggtccg gcgccgcagc cagtcgaggg cgaccagcgc ctggccgtgc tggaaggccc
     1741 ggagggtggg caggccgggc ggcggaggca ggcgggcgcc ggcgatcagg aagccggccc
     1801 aggcggtcag ggtggtggtg acggccgagg ggtcggcgcc ggcggccacc gggtgggcgg
     1861 tgaaggtcgg ctccgggtcg gggccgccct gcatggccac gctgggaagc agcaggagca
     1921 ggtcgaccca ggcggccccg acacacgccc acggccagtc gacggccacc acccgggtcg
     1981 gggtcagcag caggttgtcg gcccgcaggt cggcgtggag cagggtcggg ccccgggcgg
     2041 cctgcggcca gccggcctcc aggtcggcca gccgccgcag gtggcgggcc gcccacgggt
     2101 ccagaccggc caggtcgtcg tcgccggcgg cgtgggcggc ggccagccgg cgccagccca
     2161 cgaagctgtc cccgagccgc tcggcgacca agg
//
LOCUS       DASSWR010000645         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_729686,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000645 DASSWR010000000
VERSION     DASSWR010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729686"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..917
                     /locus_tag="VFO47_10790"
     CDS             126..917
                     /locus_tag="VFO47_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HET8789221.1"
                     /translation="MLLAHLSDTHLTTGVLAGQAAGRAHQALARVQALDPRPDCLVVT
                     GDLADHGDPAEYEAALALLGGLDLPVHVVPGNHDHAGRMLRALAGTGYVRAAAEEPDR
                     CYYRVDYPGLRLLCCDSSLPGRVDGELGAVQLAWLDRELGRDPEVPAVVALHHHPVPS
                     GIAAMDRAMLTDAGRLAAVLAGHPPPVRVLTGHLHRSTAAMFAGSLVTSAPSTNRQVL
                     LDLGPGRPLAFVDEPPGLLLHRLEGGAAMTHLVPISHSGPPIGVL"
     gene            complement(927..>2131)
                     /locus_tag="VFO47_10795"
     CDS             complement(927..>2131)
                     /locus_tag="VFO47_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8789222.1"
                     /translation="TDIGVTTEPCPGGNPDRDCIYLGVLSDLTEGPFRALAVPITEGQ
                     KAFWKRVNDQGGIGGKYEVNIETYTRDNKYNPQEHVAKYREIEPKILALAQTLGTPPT
                     LAGLPLYKEADIIAAPASWWSGWEFEDVILESGYSYCTEAMNGLDWASEEFGKPGTVM
                     AVHYPGDYGGDSAAGVAEWAKANGVDFQTEAHAVQTTPNAQAGNQDAAVERILRSDPD
                     VVMVATGPAEMAEIVGKAAAQGYKGRFIGSVPTYNPALLKSEAAPAIKALYHFVSPWG
                     PWGSDTPAHRAMQEAAGGKAPANDGYTFGWIWSYPLKAVLERAVENGDLTRAGVRAAV
                     TQVNVDYEGALPDKSYNGEPNQTAVRQALIAKPDESAPLGSSVLKDFFVGPTAEKFDF
                     TKPCVAAG"
BASE COUNT          246 a          753 c          799 g          333 t
ORIGIN      
        1 gggttcccac cgtatgcgca accattcgcc cctgcccgcc ttgcctggtt cgaaggtggt
       61 cctggccagg cagtgatcgg tggcagcatc gatgatcccc ggataccgcg accatcggag
      121 gcgccatgct gctggcccac ctgtccgaca cccatctcac caccggggtg ctcgccggcc
      181 aggcggccgg gcgggcccac caggcgctgg cccgggtcca ggcgctggac ccgcggccag
      241 actgcctggt cgtcaccggc gacctggccg accacggcga cccggccgag tacgaggcgg
      301 cgctggccct gctgggcggg ctggacctgc ccgtccatgt cgtgcccggc aaccacgacc
      361 acgccgggcg gatgctccgg gcgctggccg ggaccgggta cgtgcgggcg gcggccgagg
      421 agccggaccg ctgctactac cgggtcgact acccggggct gcggctgctc tgctgcgaca
      481 gcagcctgcc cgggcgtgtc gacggggagc tgggggccgt ccagctggcc tggctcgacc
      541 gcgagctggg ccgcgacccg gaggtgcccg ccgtggtggc cctgcaccac cacccggtcc
      601 cgagcggcat cgccgccatg gaccgggcga tgctgaccga cgccggccgg ctggcggccg
      661 tcctggccgg gcacccgccg ccggtccgcg tcctgaccgg gcacctgcac cggtccacgg
      721 cggccatgtt cgccggctcg ctggtcacct ccgcccccag caccaaccgg caggtcctcc
      781 tcgacctcgg ccctgggcgg cccctggcct tcgtcgacga gccgcccggt ctgctcctgc
      841 accggctgga gggcggcgcc gccatgaccc acctggtccc gatctcacat tccgggcctc
      901 cgatcggcgt gctctgacgg gccgggtcag ccggcggcga cgcagggctt ggtgaagtcg
      961 aacttctcgg ccgtcggccc gacgaagaag tccttgagga ccgaggagcc gagcggggcg
     1021 ctctcgtcgg gcttggcgat cagggcctgg cggacggcgg tctggttggg ctcgccgttg
     1081 taggacttgt cgggcagggc gccctcgtag tcgacgttga cctgggtgac ggcggcgcgg
     1141 acgccggcgc gggtcaggtc gccgttctcg acggcccgct ccagcacggc cttgaggggg
     1201 taggaccaga tccagccgaa ggtgtagccg tcgttggccg gggccttgcc gcccgcggcc
     1261 tcctgcatgg cccggtgggc gggggtgtcg gagccccacg ggccccaggg cgagacgaag
     1321 tggtagaggg ccttgatggc cggggcggcc tcgctcttga gcagggccgg gttgtaggtc
     1381 gggaccgacc cgatgaagcg gcccttgtag ccctgggcgg cggccttgcc gacgatctcg
     1441 gccatctcgg ccgggccggt ggcgaccatg accacgtcgg ggtcggatcg caggatccgc
     1501 tcgacggcgg cgtcctggtt gccggcctgg gcgttggggg tggtctggac ggcgtgggcc
     1561 tcggtctgga agtcgacccc gttggccttg gcccactcgg ccaccccggc ggccgagtcg
     1621 ccgccgtagt cgcccgggta gtggacggcc atgaccgtgc ccggcttgcc gaactcctcc
     1681 gacgcccagt cgagcccgtt catggcctcg gtgcagtagc tgtagccgct ctccaggatg
     1741 acgtcctcga actcccagcc cgaccaccag gaggcggggg cggcgatgat gtcggcctct
     1801 ttgtacaggg gcaggccggc cagggtcggc ggggtgccga gcgtctgggc aagggcgagg
     1861 atcttgggct cgatctcccg gtacttggcc acgtgctcct gggggttgta cttgttgtcg
     1921 cgggtgtagg tctcgatgtt gacctcgtac ttgccgccga tcccgccctg gtcgttgacc
     1981 cgcttccaga acgccttctg gccctcggtg atgggcacgg ccagggcccg gaacggcccc
     2041 tcggtgaggt cggacagcac cccgaggtag atgcagtcgc ggtcggggtt gccgcccggg
     2101 cagggctcgg tggtcacgcc gatgtcggtc t
//
LOCUS       DASSWR010000646         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_674220,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000646 DASSWR010000000
VERSION     DASSWR010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674220"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFO47_10800"
     CDS             complement(<1..488)
                     /locus_tag="VFO47_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789223.1"
                     /translation="MGGGRQAWCPRCDEVREARPGTSCVACGRELLAVPAWPGQPQPG
                     RADQVARRLRALRPAAGAAGVGLLVLAVVAGAFAAGRVTRTTPSTAAATASTGPGLAD
                     DGPEAGRRDFDGWEARAGGITVALRSLTVGTGFSRLELHVDGVPRGREVSALERLRIL
                     DAA"
     gene            complement(495..1610)
                     /locus_tag="VFO47_10805"
     CDS             complement(495..1610)
                     /locus_tag="VFO47_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET8789224.1"
                     /translation="MRTSAAARAERLARARKEQAERGVDALLLGPSADLAYLTGYHPP
                     HLERLTLLVVSAAGEARLVVPALEAPLAHGQLGDLEVEVAAWQETDDPVALVVDTLAS
                     AGATGGRLGVGDQLWSMFLLRLQEALPSARFTSASAVTGELRMRKDPEELAALARVAA
                     AIDGVVEGLGELRWAGRSEQDLARDVEEAIRATHDMFCFGIVASGPNAASPHHGATGR
                     VIQPGDPVVVDIGGRLDGYCSDTTRTLVVGEPPPGFEELYGLLRAAQEAGCAAVRPGI
                     TAAQLDAACRDPIRDGGHGDAFLHRTGHGIGLDEHEAPYIVAGNELPLEPGMAFSVEP
                     GIYLEGRYGARIEDIVVCTEDGGRRLNTTSRDLKIVS"
     gene            complement(1631..2572)
                     /locus_tag="VFO47_10810"
     CDS             complement(1631..2572)
                     /locus_tag="VFO47_10810"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789225.1"
                     /translation="MTAPLPSPAHQRERLPFGAPFLIADPAAARRDLTLLRRALAAAG
                     VVAEAAVARPVERAGAVAAQALEDGHRLLVAVGSDRLVHALLRVLVDPGGPRWPDAVL
                     GLAGLGRQDLAATFGLPDDPRKLAHHLLGPNQFLADVGRARWRGPDGTERVGVFANTA
                     ELGYPAEVSARGGGEATGRVARLAAALGGLAASRSTPARREVDHTAVDLRLAGLVVAN
                     GQFSMGRMKVAPRALPDDGRLSVMAFQGEPLGIYARSKDLYYGLHVPHPSVREYQSRR
                     VAVDTAEPLALVLDGWRAAGGPPVRFDVLERVLRIKV"
     gene            2629..>4068
                     /locus_tag="VFO47_10815"
     CDS             2629..>4068
                     /locus_tag="VFO47_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET8789226.1"
                     /translation="MDEGLAGLAAGYAFDVPAAGLGRAVKGEEVNTGFEVAVPLAMLN
                     RHGLVAGATGTGKTKTIQGLAGRLSDAGVPCFVADMKGDVSGMAEPGEASDKVAARIE
                     QLQAPWDPHPSPVELLSLSGDPAKGVPVRASVSGFGAQLLSKVLDLNDVQTSVLSLVF
                     HYADSGGLLLVDLKDLREVLKFLGTDEGQAAVADLGGVAKPTLGVLLRKLVELEEQGV
                     AGLFGEPAFDVDDLLRTDPSGRGMVTLLELADVADRPRVFSTFMMWLLAELFEALPEV
                     GDLDKPKLVFFFDEAHLLFDGASKAFVEQVQRTVRLIRSKGVGVFFITQLPQDLPDEV
                     LAQLGNRVQHALRAITPKDAAAIDKTVQTYPMTDVYDLASALTSLGIGEAVVTVLSPD
                     GVPTPVAWTRLYPPQSRMAPADPATVQSAVAGSAIHARYGQTVDPDSAYERLTARLQQ
                     AEADKPIPAPAPAPAPSAPERSGKAPGPGG"
BASE COUNT          503 a         1546 c         1539 g          480 t
ORIGIN      
        1 gcggcgtcga ggatccggag ccgttcaagg gcgctgacct cgcggccccg gggcaccccg
       61 tcgacgtgca gctccaggcg gctgaagccg gtgccgacgg tgaggctgcg gagcgcgacc
      121 gtgatcccgc cggcccgcgc ctcccagccg tcgaagtcgc ggcggccggc ctcgggaccg
      181 tcgtcggcca ggccgggccc ggtgctggcc gtcgccgccg cggtcgaggg ggtggtccgg
      241 gtcacccgcc cggcggcgaa cgcgccggcg accacggcca ggaccagcag gccgaccccg
      301 gccgcgccgg ccgccggccg cagcgcccgc agcctcctgg cgacctggtc ggcccgcccg
      361 ggctggggct ggccgggcca ggccggcacg gccagcagct cgcggccgca ggccacacag
      421 gacgtccccg gccgggcctc ccggacctcg tcgcagcgcg ggcaccacgc ctgcctgccc
      481 ccgcccatgg cgatctagct cacgatcttg aggtcgcgcg aggtggtgtt gaggcggcgg
      541 ccgccgtcct cggtgcagac gacgatgtcc tcgatcctgg ccccgtagcg gccctccagg
      601 tagatcccag gctcgaccga gaaggccatg ccgggctcca gcggcagctc gttgccggcg
      661 acgatgtagg gggcctcgtg ctcgtccagg ccgatgccgt gcccggtgcg gtgcaggaag
      721 gcgtcgccgt ggccgccgtc gcggatcggg tcgcggcagg cggcgtcgag ctgggcggcc
      781 gtgatcccgg ggcggacggc ggcgcagccg gcctcctggg cggcccgcag cagcccgtac
      841 agctcctcga acccgggggg cggctcgccc acgaccaggg tgcgggtggt gtccgagcag
      901 tagccgtcga ggcgcccgcc gatgtccacc accaccgggt cgccgggctg gatgacccgt
      961 ccggtggccc cgtggtgggg gctggccgcg ttggggccgg aggcgacgat gccgaagcag
     1021 aacatgtcgt gggtggcgcg gatcgcctcc tcgacgtccc tggccaggtc ctgctcgctg
     1081 cgcccggccc agcgcagctc gcccaggccc tcgaccaccc cgtcgatggc cgccgccacc
     1141 ctggccaggg cggccagctc ctcggggtcc ttgcgcatcc gcagctcgcc ggtgacggcc
     1201 gaggcggagg tgaaccgggc cgagggaagg gcctcctgga gccgcagcag gaacatgctc
     1261 cacagctggt caccgacgcc cagccgtccg ccggtggcgc ccgccgaggc cagcgtgtcg
     1321 accaccagcg cgaccgggtc gtcggtctcc tgccaggcgg ccacctcgac ctcgaggtcg
     1381 cccagctggc cgtgggccag gggcgcctca agggccggca ccaccagccg ggcctcgccg
     1441 gccgcggaca cgaccagcag ggtcaggcgc tccaggtgcg gcgggtggta gccggtcagg
     1501 taggcgaggt cggccgacgg ccccagcagc aacgcgtcga cgccccgttc ggcctgctcc
     1561 ttgcgggccc tggccagccg ttcggcacgg gctgcggcgc ttgtcctcat agcggcaatc
     1621 ctacctccgg tcagaccttg atccgcagca cccgttccag gacgtcgaag cggaccgggg
     1681 ggccgccggc ggcccgccag ccgtccagca ccagggcgag cggctcggcg gtgtcgaccg
     1741 cgacccggcg cgactggtac tcgcgcaccg acgggtgggg cacgtgcagg ccgtagtaca
     1801 ggtccttgga gcgggcgtag atgccgagcg gctcgccctg gaaggccatc accgacaggc
     1861 ggccatcgtc gggcagggcc cggggggcga ccttcatccg ccccatggag aactggccgt
     1921 tggccaccac cagcccggcc aggcgcaggt cgacggcggt gtggtccacc tcccgccgag
     1981 ccggggtgga gcggctggcg gccagtccgc ccagggcggc ggccaggcgg gcgacccgcc
     2041 cggtggcctc ccccccgccc cgggccgaca cctcggccgg gtagccgagc tcggcggtgt
     2101 tggcgaacac cccgacccgc tcggtgccgt cggggccgcg ccagcgggcc cggccgacgt
     2161 cggccaggaa ctggttgggc ccgagcaggt ggtgggccag cttcctgggg tcgtcgggca
     2221 ggccgaaggt ggcggccagg tcctggcggc ccaggccggc cagcccgagg acggcgtccg
     2281 gccagcgcgg cccgcccggg tcgaccagga cccggagcag ggcgtggacg agccggtcgg
     2341 agccgaccgc gaccagcagc cggtggccgt cctccagggc ctgggcggcg accgccccgg
     2401 cccgctcgac cggccgggcc accgccgcct cggcgaccac cccggcggcg gccagggcgc
     2461 ggcgcagcag ggtcaggtcg cggcgggcgg cggccgggtc ggcgatcagg aacggcgccc
     2521 cgaacggcag ccgctcccgc tggtgcgccg gcgagggcag aggtgcggtc acgccgctca
     2581 tcctatagaa cagcgcccgg agtaccggcg cgaggcgagg aggggcggat ggacgagggg
     2641 cttgccgggc tggccgccgg ctacgcgttc gacgtgccgg cggccgggct gggccgggcg
     2701 gtcaagggcg aggaggtcaa caccggcttc gaggtcgcgg tgccgctcgc catgctgaac
     2761 cggcacggcc tggtcgccgg ggccaccggc accggcaaga ccaagaccat ccagggcctg
     2821 gccgggcgcc tgtcggacgc cggggtgccc tgcttcgtgg ccgacatgaa gggcgacgtg
     2881 tcgggcatgg ccgagccggg cgaggcctcc gacaaggtgg ccgcccggat cgagcagctc
     2941 caggccccct gggacccgca tccctccccc gtcgagctgc tgagcctgtc cggcgacccg
     3001 gccaagggcg tgccggtgcg ggcctcggtg tcggggttcg gcgcccagct gctgagcaag
     3061 gtgctcgacc tcaacgacgt ccagaccagc gtgctcagcc tggtcttcca ctacgccgac
     3121 tcgggcggcc tgctcctggt cgacctcaag gacctgcgcg aggtgctgaa gttcctcggc
     3181 accgacgagg gccaggcggc cgtggccgac ctgggcgggg tggccaagcc gaccctcggg
     3241 gtgctgctgc gcaagctggt cgagctggag gagcagggcg tggccgggct cttcggcgag
     3301 cccgccttcg acgtcgacga cctgctgcgg accgacccct cgggcagggg gatggtgacg
     3361 ctgctggagc tggccgacgt ggccgaccgg ccgcgggtgt tctcgacctt catgatgtgg
     3421 ctgctggccg agctgttcga ggccctgccc gaggtcggcg acctcgacaa gcccaagctg
     3481 gtgttcttct tcgacgaggc ccacctgctg ttcgacggcg cctccaaggc gttcgtcgag
     3541 caggtgcagc ggacggtgcg gctgatccgc tccaaggggg tcggggtgtt cttcatcacc
     3601 cagctccccc aggacctccc cgacgaggtc ctggcccagc tcggcaaccg ggtccagcac
     3661 gccctgcggg cgatcacccc caaggacgcg gccgccatcg acaagacggt ccagacctac
     3721 ccgatgaccg acgtctacga cctggcctcg gccctgacct cgctgggcat tggcgaggcg
     3781 gtggtgaccg tgctgtcccc cgacggtgtc cccaccccgg tcgcctggac ccgcctgtac
     3841 ccgccccagt cccgcatggc cccggcggac ccggccaccg tccagtcggc cgtcgccggc
     3901 tcggccatcc acgcccgcta cggccagacc gtcgatcccg acagcgccta cgagcgcctc
     3961 accgcccgcc tccagcaggc cgaggccgac aagccgatcc cggcccccgc gccggcgccg
     4021 gccccttcgg cccccgagcg gtccggcaag gccccgggcc cgggcggc
//
LOCUS       DASSWR010000647         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_504774,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000647 DASSWR010000000
VERSION     DASSWR010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504774"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1122
                     /locus_tag="VFO47_10820"
     CDS             169..1122
                     /locus_tag="VFO47_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8789227.1"
                     /translation="MDGDTRYSIGDLARRTGLSVRTIRFYSDRGLLPPTSRSPAGYRR
                     YDLDALARLDLVRTLRDLGVDLATIGRVLDREISVPEVAAAHAEALDAQIRTLRLRRA
                     VLRAVAKRGSSPQEMELMHKLAKLSDAERRRIIHQFIDEAFGDLDANPDLVAMLRSAM
                     PELSDDPTPEQVDAWVELAELVQDPDFKASVRRAAEHQAADRADGDQSGLHHELTNQI
                     RERVEAARADGIDPASEQARLVLADLVGRYAQTFGTADTPDYRSKLLLRLQIASDPRV
                     ERYWHLLATINGWPIPPTLAPVFEWFIQALQHHPDPTTTPA"
     gene            complement(1190..1747)
                     /locus_tag="VFO47_10825"
     CDS             complement(1190..1747)
                     /locus_tag="VFO47_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET8789228.1"
                     /translation="MAKLIYSAITSLDGYVADAEGRFDWAAPDEEVHAFVNDLERTVG
                     TYLYGRRMYEVMRAWETLPLAGQPPSMRDYAEIWRAADKIVYSRTLEDVASARTRIER
                     DFDPGAVRELKASAGADLSVGGPHLAARALEAGQVDEVHLFLNPVVVGGGNPAFPGGL
                     RLELELLGERRFGNGVVHLHHRVSG"
     gene            complement(1934..>2599)
                     /locus_tag="VFO47_10830"
     CDS             complement(1934..>2599)
                     /locus_tag="VFO47_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HET8789229.1"
                     /translation="SYLVDQKRAREAGRELFDAVYDRWSRLPADDRPKLFVFGLSLGS
                     FGGETAFSGEYDLRNRTAGALFAGPPNFNLLYREFTDDRDPGSREVEPVYRDGRSVRF
                     TNDVAAGVGPDGPWQGTRVLYLQHPSDPIVWWSPRLVWDRPDWLGEPPGRDVLDAMLW
                     IPFVTFWQVTADMPLAGAGPAGHGHAYEAEFVDGWEEVIQPSGLTPDKVERLRDIVTE
                     ESG"
BASE COUNT          397 a          943 c          914 g          345 t
ORIGIN      
        1 cttgccgtcg aagtccttgc cgtcgaagtc ggcgaaccct gatgtcagac agggcacgat
       61 gcggcgggac agcttggacc agatgaccag ctgggctggc cgaccagggt gaccggagtc
      121 gggaaatagc cttgcgtctc cagctggtgg aggctggaag atgcgggcat ggacggcgac
      181 acgcgctact cgatcgggga tctggccaga cggaccgggc tgtcggtgcg cacgatccgg
      241 ttctactccg accgcggtct gctgccgcca accagccgta gccccgccgg ctaccgccgc
      301 tacgacctgg acgcactggc ccgtctggac ctggtccgca cgctgcggga tctcggtgtg
      361 gatctcgcca ccatcgggcg ggtgctggac cgcgagatct cggtgcccga ggtcgctgcc
      421 gcacatgccg aggcgctgga cgcgcagatc cgtaccttgc ggctgcgccg agctgtcctg
      481 cgtgcggtgg ccaagcgcgg gtccagtccc caggagatgg aactcatgca caagctggcc
      541 aagctctcgg acgccgagcg gcgccggatc atccaccagt tcatcgacga ggccttcgga
      601 gacctcgacg ccaaccccga cctggtggcc atgttgcgct cggcgatgcc cgagctttcc
      661 gacgacccca cccccgagca ggtcgacgcc tgggtcgagc tggccgaact ggtccaggac
      721 ccggacttca aggcgagcgt ccggcgggcg gccgaacacc aggcggccga ccgggccgac
      781 ggcgaccagt cggggctgca ccacgagctg accaaccaga tccgagaacg ggtcgaggcg
      841 gcgcgagctg acgggatcga cccagcgtcc gagcaggccc gcctggtcct ggccgatctg
      901 gtcggccgct acgcccaaac cttcggcacg gccgacaccc ccgactaccg cagcaagctg
      961 ctgctccgac tgcagatcgc cagtgaccca cgggtcgagc ggtactggca cctgctggcc
     1021 accatcaacg gctggcccat tccaccgacc ctggctccgg tgttcgagtg gttcatccaa
     1081 gcgctccagc accaccccga cccgacgaca accccagcat gagcccgcct ggccccgccc
     1141 gaccacggac ggggccagac gccggccagg cccgcgcccg ccggagccgc tagcccgaca
     1201 cgcggtggtg gaggtggacg acgccgttgc cgaagcggcg ctcgccgagc agctccagct
     1261 ccaggcggag gccgcccggg aaggccgggt tgccgccgcc gacgacgacg gggttgagga
     1321 acaggtggac ctcgtcgacc tgcccggcct cgagcgcccg ggcggccagg tggggaccgc
     1381 ccacgctgag gtcggcgccg gccgacgcct tcagctcgcg gaccgctccg gggtcgaagt
     1441 cccgctcgat ccgggtccgg gcgctggcca cgtcctccag ggtcctggag tagacgatct
     1501 tgtcggccgc ccgccagatc tccgcgtagt cccgcatgga gggtggctgg ccggccaggg
     1561 gcagggtctc ccaggcgcgc atcacctcgt acatgcggcg cccgtagagg taggtcccca
     1621 ccgtccgctc caggtcgttg acgaaggcgt gcacctcctc gtccggcgcg gcccagtcga
     1681 acctgccctc ggcgtccgcc acgtagccgt cgagcgacgt gatcgcggag tagatcagct
     1741 tggccatggc cagaagtatg cgcgattcac cccggcaggt gaggacggcc cacccggcgc
     1801 ccgcagaatc gacgacgccg gcccgacccc ggcgaacggg aacagggcca ccaccgctcg
     1861 cggtcccgcg cacagccgtt cgctgaccgg ggccggtcgt cgtcaccctc accgggtgat
     1921 ttcgcgacgg cggctacccg ctctcctcgg tgacgatgtc gcgcaggcgc tcaaccttgt
     1981 cgggggtgag gccggacggc tggatgacct cctcccagcc gtcgacgaac tcggcctcgt
     2041 aggcgtgccc gtggccggcg gggccggcgc cggccagggg catgtcggcg gtgacctgcc
     2101 agaaggtcac gaacgggatc cacagcatcg cgtccagcac gtcgcggccg ggcggctcgc
     2161 ccagccagtc ggggcggtcc cagaccaggc gggggctcca ccagacgatc gggtccgaag
     2221 ggtgctggag gtacagcacc cgcgtcccct gccaggggcc gtccggcccg accccggcgg
     2281 cgacgtcgtt ggtgaagcgg accgagcggc cgtcccggta gaccggctcg acctcccggc
     2341 tccccgggtc gcggtcgtcg gtgaactcgc ggtagagcag gttgaagttg ggcggcccgg
     2401 cgaacagcgc cccggcggtc cggttgcgca ggtcgtactc gccgctgaag gcggtctcac
     2461 ccccgaagga gccgaggctg aggccgaaca cgaacagctt ggggcggtcg tcggccggca
     2521 gccgggacca gcggtcgtag acggcgtcga acagctcccg gcctgcctcc cgggcccgct
     2581 tctggtcgac gagatagga
//
LOCUS       DASSWR010000648         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1183532, whole genome shotgun sequence.
ACCESSION   DASSWR010000648 DASSWR010000000
VERSION     DASSWR010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183532"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..645)
                     /locus_tag="VFO47_10835"
     CDS             complement(28..645)
                     /locus_tag="VFO47_10835"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET8789230.1"
                     /translation="MTLADPVGAQPGRGLRILVVDNYDSFVFNLVQYLGELGAETVVW
                     RNDAGTVDDVRALDPDGVVISPGPGHPRDAGLSVDIVRDLGAQLPVLGVCLGHQVIGH
                     VYGGRVSRAPELVHGKTSPVHHQGAGVLAGLPAPFAATRYHSLVVERDGLPEVLEVTG
                     ETAQGLVMALRHRRHPVEGVQFHPESILTDAGHSLLGNFLGVVRA"
     gene            complement(642..2357)
                     /locus_tag="VFO47_10840"
     CDS             complement(642..2357)
                     /locus_tag="VFO47_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET8789231.1"
                     /translation="MPETTTPWLDNYPPGVPEHVELPKASLAGLLEDAARDFPHAPAL
                     HFEGRTISYAQLAQQARRFSGVLAGLGVGRGTRVGLILPNCPQAVVALFGALRLGATV
                     VQNNPLYTERELGHQLADAGVEVLVCLDLVYDRVKALRPRTAIREVIVTSVLDELPGF
                     KRLLAPYTKKGKAASASIGKDEPVRRWRDVLGSAPAAPGLVEVDADRDLALLQYTGGT
                     TGVSKGVMLTHGNLRANVEQVRAWFPDADPGREVMMAVLPFFHVYGLTVCLLFGLRIG
                     AALVLLPRFDLDGVLAAVDKYRPTLFPGVPTMYVAINNAVEKGGHDLSSIKACLSGAA
                     ALPLEVAERFERFSGGRLVEGYGLSESSPVAIANPIYGKRKAGTIGMPLPDTLARVSD
                     PDDPSRTMPVGEPGELALAGPQVMIGYWNRPDETAQVLRDGWLLTGDMAVMDEEGYFS
                     IVDRKKDLIIAGGYNIYPREVEEVLYEHPKILEVCVAGVPDNYRGEVVKAFVVLRSGE
                     QATSDEIREFAKARLAAYKVPRSVEFRDELPKTLIGKVLRRALVEEEQARQEKQKAES
                     GAETP"
     gene            complement(2463..>3416)
                     /locus_tag="VFO47_10845"
     CDS             complement(2463..>3416)
                     /locus_tag="VFO47_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789232.1"
                     /translation="QQDPIWEAPPAAEPLAEQGPPDPQPASWPFEETTQSWEPSDGSF
                     VWPADELPSTSAEWNTPAPPSWLDDPGAGAAPPPAAPSAHPSTASPSEPDLDQTAAWS
                     SPWADQPDAGNGLPPAPAGPPFASPGPAVVPPGPPPMVPPGPPPADWASAIPTDVPAA
                     RRAADPASTRAWRPDEGVEGEPGVPLGPGPRPPAGEGAASRMARLRGPGEEGAASHAS
                     RLQAAAGQAPASPMADPGFASRDPVSRAFGTRAADRAVPVPEIEPDEEPRERRRDRQP
                     GERQARAWPRIVAVISWIVLVMVVCWFYVFPWLERVLPENF"
BASE COUNT          453 a         1229 c         1299 g          435 t
ORIGIN      
        1 gcgcggccgg gcccgcctgc cctgagctca ggctcggacg acacccagga agttgccgag
       61 gagcgagtgg ccggcgtcgg tgaggatcga ctcggggtgg aactggacgc cctcgaccgg
      121 gtggcggcgg tggcgcaggg ccatgaccag gccctgggcg gtctccccgg tcacctccag
      181 cacctcgggc aggccgtcgc gctcgaccac cagggagtgg tagcgggtgg ccgcgaacgg
      241 cgccggcagc ccggccagga ccccggcccc ctggtggtgg acgggcgagg tcttgccgtg
      301 gaccagctcg ggagcgcggc tgacccgtcc gccgtagacg tggccgatga cctggtggcc
      361 gaggcagact ccaaggacgg gcagctgggc ccccaggtcg cggacgatgt cgaccgaaag
      421 gccggcgtcg cgcgggtgcc ccggccccgg ggagatcacc acaccgtccg ggtcgagggc
      481 gcgaacgtcg tcgaccgtgc cggcgtcgtt gcgccacacc accgtctccg cccccagctc
      541 ccccaggtac tggaccaggt tgaagacgaa cgagtcgtag ttgtccacca ccaggatccg
      601 cagcccccgg cctggctgcg cgccgacagg gtcggcgagg gtcatggcgt ctcggctccg
      661 ctctcggcct tctgcttctc ctgccgggcc tgctcctcct cgaccagggc acggcgcagg
      721 accttgccga tcagggtctt gggcagctcg tcgcggaact cgaccgaccg ggggaccttg
      781 taggcggcca ggcgggcctt ggcgaactcg cggatctcgt cgctggtcgc ctgctcgccc
      841 gagcgcagga ccacgaacgc cttgacgacc tcgccccggt agttgtcggg caccccggcc
      901 acgcacacct cgaggatctt ggggtgctcg tagaggacct cctcgacctc gcgcgggtag
      961 atgttgtagc cgccggcgat gatcaggtcc ttcttgcggt cgacgatcga gaagtagccc
     1021 tcctcgtcca tcaccgccat gtccccggtg agcagccagc cgtccctgag cacctgggcc
     1081 gtctcgtcgg gacggttcca gtagccaatc atgacctgcg ggccggccag ggccagctcg
     1141 cccggctcgc ccaccggcat ggtccgagac gggtcgtcgg ggtcggacac ccgggccagg
     1201 gtgtcgggca gcggcatgcc gatggtgccg gccttgcgct tgccgtagat cgggttggcg
     1261 atggccaccg gcgaggactc cgacaggccg tagccctcga ccaggcgccc cccggagaag
     1321 cgctcgaacc gctcggcgac ctccagcggc agggcggccg cgccggacag gcaggccttg
     1381 atgctggaca ggtcgtggcc gcccttctcg acggcgttgt tgatggccac gtacatggtg
     1441 ggcacgcccg ggaacagcgt cggccggtac ttgtcgaccg ccgccagcac gccgtcgagg
     1501 tcgaagcggg gcagcaggac cagggcggcc ccgatgcgca ggccgaacag caggcagacg
     1561 gtgagcccgt acacgtggaa gaagggcagc acggccatca tgacctcgcg gccggggtcg
     1621 gcgtcgggaa accaggcccg gacctgctcg acgttggccc ggaggttgcc gtgggtgagc
     1681 atcacgccct tggagacgcc ggtggtgccg ccggtgtact gcagcagggc caggtcgcgg
     1741 tcggcgtcga cctcgaccag accgggggcg gccggagccg acccgagcac gtcgcgccag
     1801 cgcctgaccg gctcgtcctt gccgatgctg gcgctggccg ccttgccctt cttggtgtag
     1861 ggggcgagca gccgcttgaa ccccggcagc tcgtcaagga ccgaggtgac gatcacctcg
     1921 cggatggcgg tcctggggcg cagcgccttg acccggtcgt agaccaggtc aaggcagacc
     1981 agcacctcga ccccggcgtc ggcgagctgg tgaccgagct cgcgctcggt gtagaggggg
     2041 ttgttctgga ccacggtcgc ccccaggcgc agcgccccga acagggccac caccgcctgc
     2101 gggcagttgg gcaggatcag cccgaccctg gtgccgcggc caaccccgag cccggcgagc
     2161 acgccggaga agcgccgggc ctgctgggcc agctgggcgt aggagatggt gcggccctcg
     2221 aagtggaggg ccggcgcgtg ggggaagtcc cgggcggcgt cctcgagcag gccggccagg
     2281 ctggccttgg gcagctccac gtgctcgggg acgcccggcg ggtagttgtc gagccaaggc
     2341 gtcgtcgtct cgggcatcgt gggtcccacc tcgtttcggc agccggcttg gcaagcagta
     2401 tcggcgtcgc tcgccgtcag gacaatctac ctgcgctggc aagacaagct cggcgtccgg
     2461 gatcagaagt tctctggaag cacccgctcc agccacggga acacgtagaa ccagcagacc
     2521 accatgacca gcacgatcca ggagatcacg gccacgatcc gcggccaggc cctggcctgg
     2581 cgctcgccgg gctgccggtc ccgccggcgc tcgcgcggct cctcgtccgg ctcgatctcc
     2641 ggcaccggga cggcccggtc ggcggcccgg gtcccgaacg cccgcgacac cgggtcccgc
     2701 gacgcgaacc cgggatcggc catgggcgac gctggcgcct gaccggccgc cgcctggagg
     2761 cgggaagcgt gcgacgccgc cccctcctcg cccggcccgc gcagcctggc catgcgcgac
     2821 gccgctccct cgccggccgg cggccgggga ccggggccga ggggcacgcc cggctcgccc
     2881 tcgaccccct cgtcgggccg ccaggcccgg gtggacgccg ggtcggccgc ccgccgggcc
     2941 gccggcacgt cggtcgggat ggccgaagcc cagtccgccg gcggcggccc cggcggcacc
     3001 atcggcggcg gcccgggcgg cacgacggcc ggccccggcg aggcgaaggg cggtccggcg
     3061 ggggccgggg gcaggccgtt gccggcgtcc ggctggtcgg cccacggcga ggaccaggcg
     3121 gccgtctggt ccaggtccgg ctcggacggg gaagcggtcg aggggtgagc ggagggggcg
     3181 gcgggcggcg gggccgcgcc ggcgcccggg tcgtccagcc aggacggcgg ggcgggtgtg
     3241 ttccactcgg ccgaggtcga gggcagctcg tcggccggcc agacgaagga gccgtcgctc
     3301 ggctcccagc tctgggtggt ctcctcgaac ggccaggagg ccggctgcgg gtcgggcggc
     3361 ccctgctcgg ccagcggctc ggcagccggc ggcgcctccc agatcgggtc ctgctg
//
LOCUS       DASSWR010000649         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1240336, whole genome shotgun sequence.
ACCESSION   DASSWR010000649 DASSWR010000000
VERSION     DASSWR010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240336"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..466
                     /locus_tag="VFO47_10850"
     CDS             272..466
                     /locus_tag="VFO47_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789233.1"
                     /translation="MAALLPPDPTDRPATTAQVLALLAAAMPADAAEEDRLWPTWATG
                     RLAEWQASTMARPPLVPRPA"
     gene            695..>2027
                     /locus_tag="VFO47_10855"
     CDS             695..>2027
                     /locus_tag="VFO47_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HET8789234.1"
                     /translation="MIGRIVIEDIRPQVGCGQFTARAVAGETVPVSATIFRDGHNEVV
                     AAAVRLTGPGDRKGQLVPLVEVNHGLHQWQGEITLPRTGAWSMVVEAWTDEVATWRIG
                     TQRKLDAGRDIGLELEEGARLVEAARAWVPASGREVLTAAAAGLRNEGADPAERLAAA
                     SDSTVVELLERHPPKEATRSKRLGIWVDRPRALYGAWYEFFPRSEGATATTSGTFKQA
                     AERLPAIAAMGFDVVYLPPIHPIGHTHRKGPNTPLEPGPDDPGSPWAIGSEAGGHDAI
                     HPDLGTIDDFDAFVARAGELGMEVALDYALQCSPDHPWVSDHPDWFRIRPDGSIAYAE
                     NPPKVYQDIYPLNFDSPDAPALYEACKGVLDHWIAHGVRIFRVDNPHTKPVRFWEWLI
                     GQVKAEHPDVIFLAEAFTRPPMMRVLAKVGFTQSYTYFTWRNAKEELEEYCN"
BASE COUNT          276 a          803 c          708 g          240 t
ORIGIN      
        1 gcctggcccg gccggtgggc gaggcgcccg ccggcggaca ccgccgcggc tcgccgccgt
       61 ggatggcccc cgagcaggtc cgccgccgtc cggccagccc gggcatggac ctgttcgcgc
      121 tcggagcggt cctgtacgag ctggccacgg gcacccaggc gttcgacccg gccgaccacg
      181 gcccgcccgg gcgccgctgg ccccagctgg ccggcccccc acccccggcg agcagccaca
      241 acccggaggt gccggcggcg tcgaccgggc cgtggccgcc ctgctgcccc ccgacccgac
      301 cgaccggccg gccaccaccg cccaggtgct cgccctgctg gccgcggcca tgccggccga
      361 cgccgccgag gaggaccggc tgtggcccac ctgggctacc gggcgcctcg cggagtggca
      421 agcgtccacc atggcgcgtc ccccgctggt tccacgacct gcgtaggcat tgcaggtggc
      481 ccagagatgc tccttgtcag gatggttgcg acgggaaaga ctgcggctcg ccctggggag
      541 cgatcccaag ggcgggccct cgtgggggcc agggggggcc gcgggcgggt gcgcgcgctc
      601 acccggcgcc gggcccccac gggggcgctg aactcccagc gccctgagcc gttccgccgc
      661 tgaaccgggc gccccgcagt ggtagcgtgc tcacatgatc gggcgcatcg tgatcgagga
      721 catccgtcct caggtcggct gcgggcagtt caccgcccgg gcggtcgccg gggagacggt
      781 cccggtctcc gcgaccatct tccgcgacgg ccacaacgag gtcgtggccg cggccgtgcg
      841 cctgaccggc ccgggcgacc gcaagggcca gctcgtcccc ctggtcgagg tcaaccacgg
      901 cctgcaccag tggcagggcg agatcaccct gccccgcacc ggcgcctggt cgatggtggt
      961 cgaggcctgg accgacgagg tcgccacctg gcgcatcggc acccagagga agctcgacgc
     1021 cggccgcgac atcggcctcg agctcgagga gggcgcccgc ctggtggagg cggcccgggc
     1081 ctgggtgccg gcgtccggtc gcgaggtgct cacggcggcc gcggccgggc tgcgcaacga
     1141 gggcgccgac ccggccgagc ggctggccgc cgcctccgac tcgaccgtgg tcgagctgct
     1201 ggagcggcac ccgcccaagg aggccacccg ctccaagcgc ctcggcatct gggtcgaccg
     1261 cccccgcgcc ctctacggcg cctggtacga gttcttcccc cgctccgagg gggccacggc
     1321 caccaccagc ggaacgttca agcaggcggc cgagcggctc ccggccatcg ccgccatggg
     1381 cttcgacgtg gtctacctgc cgccgatcca ccccatcggc cacacccacc gcaagggccc
     1441 caacaccccc ctggagcccg gcccggacga ccccgggagc ccatgggcga tcggctcgga
     1501 ggccggcggc cacgacgcca tccaccccga cctgggcacc atcgacgact tcgacgcctt
     1561 cgtggccagg gccggcgagc tgggcatgga ggtcgcgctc gactacgccc tgcagtgctc
     1621 gcccgaccac ccctgggtca gcgaccaccc ggactggttc cgcatccggc ccgacggctc
     1681 gatcgcctac gccgagaacc cgcccaaggt ctaccaggac atctacccgc tgaacttcga
     1741 cagcccggac gcccccgccc tgtacgaggc ctgcaagggc gtgctggacc actggatcgc
     1801 ccacggggtg cggatcttcc gggtcgacaa cccccacacc aagccggtgc ggttctggga
     1861 gtggctgatc gggcaggtca aggccgagca cccggacgtg atcttcctgg ccgaggcgtt
     1921 cacccgcccg ccgatgatgc gggtcctggc caaggtcggc ttcacccagt cctacaccta
     1981 cttcacctgg cgcaacgcca aggaggagct ggaggagtac tgcaacg
//
LOCUS       DASSWR010000650         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_392136,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000650 DASSWR010000000
VERSION     DASSWR010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392136"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFO47_10860"
     CDS             complement(<1..113)
                     /locus_tag="VFO47_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET8789235.1"
                     /translation="MIASLRGRLLEVLADGAVIEVGGVGYRVHLTPKAAAGL"
     gene            complement(110..691)
                     /gene="ruvC"
                     /locus_tag="VFO47_10865"
     CDS             complement(110..691)
                     /gene="ruvC"
                     /locus_tag="VFO47_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET8789236.1"
                     /translation="MRVLGVDPGLGRCGWAVLEGRRGRVTAAGYGTITTDGEQVAPRL
                     AALAARLREVLAAHRPEALAIERLFFNANVRTAMTVGQASGVVLLLAAEHGLEVTAYT
                     PPQVKQAVTGSGSAPKEQVGYMVKALLGLTSVPAPADTADALAVALCHLNHAGLAGAG
                     PGDGGGSIGAASTADWLARAAPTSPGLAKGSGR"
     gene            complement(1001..1747)
                     /locus_tag="VFO47_10870"
     CDS             complement(1001..1747)
                     /locus_tag="VFO47_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8789237.1"
                     /translation="MSGHSKWSTIKHQKGAKDARRGKLFARLIRAIEVAAREGGSNPD
                     GNPTLADAIQKAKDNSVPNDTIDRAVKRGAGELGGQRLELATYEAYAPGGVALMVEAL
                     TDNRNRTAADVRHVVTKGGGSLADAGSVAYLFHRRGQLVVLGDDVDEDRVMEVALEAG
                     AEDVEQGDQGFTVTTAPGDLRAVRAAFDAAGIAYDSAEVTMVPSMSVPLDRDVALRVL
                     RLLDALEDLDDVQDVYANFDIPDEVMAEVS"
     gene            complement(1744..2334)
                     /gene="pdxT"
                     /locus_tag="VFO47_10875"
     CDS             complement(1744..2334)
                     /gene="pdxT"
                     /locus_tag="VFO47_10875"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET8789238.1"
                     /translation="MPAPRVGVLAFQGDVREHLAALAAVGADPVEVRTMADLDSVEGL
                     VVPGGESTVIGKLAARYGLLAPLRARVADGLPVLGTCAGMIFLAREVEGPPQDLLGVL
                     DVRVRRNAFGRQVASFEAGVDVKGVDGGPVSGAFIRAPWVAEAAPEVEVLAEVDGKVV
                     AVRQGNLLATAFHPELTGEVRLHRWLVDLVAERSSS"
     gene            complement(2343..>2569)
                     /locus_tag="VFO47_10880"
     CDS             complement(2343..>2569)
                     /locus_tag="VFO47_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949218.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET8789239.1"
                     /translation="ADAAMMMQLGADGVFVGSGIFKSGDPARRARAIVEATTFSRDPE
                     IVAKVSRGLGEPMVGIAAVPEGERLADRGW"
BASE COUNT          313 a          972 c          947 g          337 t
ORIGIN      
        1 agcccggcgg ccgccttggg ggtcaggtgc acccggtacc cgaccccacc gacctcgatc
       61 acggccccgt cggccaggac ctcgagcagc cgcccgcgca ggctggcgat catcgcccgc
      121 tccccttggc cagccccggc gaggtcgggg cggccctggc cagccagtcg gcggtcgagg
      181 cggcgccgat cgacccgccc ccgtcccccg ggccggcgcc ggccaggccg gcgtggttga
      241 ggtggcagag ggcgacggcc agggcgtcgg cggtgtcggc cggggccggg accgaggtca
      301 gcccgaggag cgccttgacc atgtacccga cctgctcctt gggcgcgctc ccgctgccgg
      361 tgacggcctg cttgacctgc ggcggggtgt aggcggtgac ctcaaggccg tgctcggcgg
      421 ccagcagcag caccaccccg gaggcctggc cgacggtcat ggccgtgcgc acgttggcgt
      481 tgaagaagag ccgctcgatg gccagcgcct ccggccggtg ggcggccagc acctcccgca
      541 gccgcgccgc cagggcggcc agccggggcg cgacctgctc gccgtcggtg gtgatggtcc
      601 cgtacccggc ggccgtcacc cggcccctgc gcccctcgag cacggcccac ccgcagcgac
      661 cgagcccggg atcgaccccg agcacccgca tggccggccc tccgcttctc gaacaggtgt
      721 ttggcggcag cctaccccct gcccccgtca ggctcaacca tccccgcccg gtttggttgg
      781 ttagcctccg ccccggacgg gtaggcggcg ctcgacccga agggaggcga gatgcggctc
      841 ggtaccctgc tcttgatcat catcatcgtg ctgctggtgc tgttcctgtt cagccgccgt
      901 cgcggccgcc tgtaacccgg tcccgcgccc ggccggcggg gagcagcgcg ccggccgggg
      961 ctggcaagga ggccggcttc cgccccctca tccgtgacgg tcagctgacc tcggccatga
     1021 cctcgtcggg gatgtcgaag ttggcgtaga cgtcctggac gtcgtcgagg tcctcgaggg
     1081 cgtcgaggag gcgcaggacc cggagggcca cgtcgcggtc caggggcacc gacatgctgg
     1141 ggaccatggt gacctcggcc gagtcgtagg cgatgccggc ggcgtcgaag gcggcccgga
     1201 cggcgcgcag gtcgccgggg gcggtggtga cggtgaagcc ctggtcaccc tgctcgacgt
     1261 cctcggcgcc ggcctccagg gcgacctcca tgacccggtc ctcgtcgacg tcgtcgccga
     1321 gcacgaccag ctggccgcgg cggtggaaga ggtaggccac gctgccggcg tcggccaggc
     1381 tgccgccgcc cttggtgacc acgtggcgga cgtcggcggc ggtgcggttg cggttgtcgg
     1441 tcagggcctc gaccatcagc gccaccccgc cgggggcgta cgcctcgtag gtggccagct
     1501 cgaggcgctg gccgcccagc tcgccggcgc cccgcttgac ggcccggtcg atggtgtcgt
     1561 tgggcaccga gttgtccttg gccttctgga tggcgtcggc cagggtcggg ttgccgtcgg
     1621 ggttgctgcc gccctcgcgg gccgcgacct cgatggcccg gatcagcctg gcgaacagct
     1681 tgccccggcg ggcgtccttg gcgcccttct ggtgcttgat ggtcgaccac ttcgaatgcc
     1741 cgctcatgac gaactcctct ccgcgaccag gtcgaccagc cagcggtgca ggcgcacctc
     1801 gccggtcagc tcgggatgga aggcggtggc gagcaggttg ccctggcgca cggccaccac
     1861 cttgccgtcg acctcggcca gcacctcgac ctcgggggcg gcctcggcca cccagggggc
     1921 gcggatgaac gcgcccgaca ccgggccgcc gtccacgccc ttgacgtcca ccccggcctc
     1981 gaaggaggcc acctggcggc cgaaggcgtt gcggcggacc cgcacgtcca ggacacccag
     2041 caggtcctgg ggcggcccct cgacctcgcg ggccaggaag atcatgccgg cgcaggtccc
     2101 gagcaccggc aggccgtccg cgacccgcgc ccgcagcggc gccagcagcc cgtagcgggc
     2161 ggccagcttg ccgatcacgg tcgactcgcc cccgggcacg accaggccct ccacggaatc
     2221 gaggtcggcc atggtgcgga cctcgaccgg gtcggccccg acggcggcca gggcggccag
     2281 gtgctcgcgg acgtcgccct ggaaggccag gacgccgacc ctgggtgcgg gcatcgggct
     2341 ccctaccagc cgcggtcggc cagccgctcg ccctcgggca cggcggcgat gccgaccatc
     2401 ggctcgccga gcccgcgcga caccttggcc acgatctcgg ggtcgcggga gaaggtggtc
     2461 gcctcgacga tggcccgggc ccggcgggcc gggtcgccgg acttgaagat gccggagccg
     2521 acgaacaccc cgtcggcgcc gagctgcatc atcatggccg cgtcagccg
//
LOCUS       DASSWR010000651         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_307131,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000651 DASSWR010000000
VERSION     DASSWR010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307131"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..417
                     /locus_tag="VFO47_10885"
                     /pseudo
     CDS             139..417
                     /locus_tag="VFO47_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001189.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(436..1884)
                     /locus_tag="VFO47_10890"
                     /pseudo
     CDS             complement(436..1884)
                     /locus_tag="VFO47_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947897.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            1978..>3407
                     /locus_tag="VFO47_10895"
     CDS             1978..>3407
                     /locus_tag="VFO47_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET8789240.1"
                     /translation="MTGAAPSGGADRPVRLSAAELIDAVVDPGSWRSWDEPAVAPHGL
                     DPSYAADLARARERTGLDEAVLTGTGRLRGRQVAVVVSEFGFLAGSIGVAAGERLVRA
                     VERAGRERLPLVAAPASGGTRLQEGTVAFLQMVKVAAAIADHRRAGLPYLVYLRHPTT
                     GGVLASWGSLGHVTAAEPGALIGFLGPRVYEALHDQPFPPDVQVAENLYAKGLVDAVV
                     APGEVAEVAARVLDVLCAPREVPQAAEPPADDQAGETPVAESIRRSRRPDRPGVRALL
                     RVAARNVTPLSGTAAGEMDPGVLLVLARFGAVPCILLGQDRHRQRRQQPLGPAGLRAA
                     RRGIRIATELDLPLVTIVDTAGADLSKEAEEGGLAGEIARCLADLVTLPAPTLCLLLG
                     EGAGGGALAFLPADRVVAAEHAWLSPLPPEGASAVLYRTTERAVEVAERQGIGVAALR
                     AHRIVDRVVPELPDAADEGEAFLQRVG"
BASE COUNT          411 a         1236 c         1286 g          474 t
ORIGIN      
        1 cccagcctcg actccctcac cggcgccggt ggtgtcggtg ttggccgccc ggtcctgccg
       61 gtcaatatcg gggggctggc gctcgcggcg gccttcctgg tcggcaccct ggtcgcaggc
      121 cagtccaccg acgacatcat cgggttcgtg gccatcacca tcggcctggt cctcaccctg
      181 atctcgccac agctgcagca gcgcgcggtc gggcaggtca cctggccgga gatcctgctg
      241 atcaccgggt cggcacctac gtcggcgtgc tgcaggagat gggcaccatc gatggccaac
      301 gccccccaga gtgtcgaccg ggaccgcttc ttccggcagc tggtggccta cggcgccctg
      361 gttaccgtgc tcgccccgat cgtgctctgg ttggtgttcg tggtcgtcct gggctagggg
      421 cggagctgag tccttgaggt cgcgcttcat gaccttgccc atgtcgttgc ggggcagctg
      481 gtccaggtag cggaccaccc gggggcgttt gtgcgggccg agcagccgcg caacgtggcc
      541 gatcagctcc tgctcgggcg gccgcgcctg ggccgggacg atccaggcga cgatgtgctc
      601 ccccaggtcg ggatcgggct ccccggtcac cgccgcctcg gcgacccccg ggtgctcgcc
      661 cagagcgctc tcgatctcgc cggcgccgat cttgtacccg ccgctcttga tcaggtcgac
      721 cgagcggcgc ccgacgatcc ggatgtagcc gtcgggggcg cgcacggcca catccccggt
      781 gtggaaccag ccgtcgcgga ccgcctcggc ggtggcgtcc gggcggttga ggtactccag
      841 gaacagattc ggcccccgca ccaggatctc gccgacggtc tggtcgtcgc tggcttggat
      901 ggtcgcgccg tcgtcatcga tcagccgcac ctccaccccg tcgaggggcg gtccgacggt
      961 gccgggacgg cggtcgccgg cggcacgcac ggcacagttc atcagcgtct cggtcatgcc
     1021 gtagcgctcg acgacgcgct ggccggtggc ggccgcgatc cgctcgtggt cggtggccgg
     1081 cagcgccgcc gatccggaga ccagcagtcg tgcccgcgag atcccgccgg cggtctccgg
     1141 atcgcgttcg gcgtcggcag ccaaccggtg gtacatggtg gggacgccga acatcatcgt
     1201 cgccggcccg gtcagctcgg cggccaccgc cgccgacgag aagcgctcca ggtggtggac
     1261 ggctccgccg aggcggaggg gtccgagcac gccaaggatc agcccgtgga cgtggaacag
     1321 cggcagggcg tgggcgagga cgtcgtcgcc cgtccattcc caggcggcgg ccagcgcgtc
     1381 caggttggac gagatcgccc gcctgggcaa cgcgacaccc ttgggcggcc cggtcgtccc
     1441 cgaggtgtag acgatcaccg ccggagcctc gggcgagggc tcggcgggca gggcaccgcc
     1501 ccgggccgac agatcgacgt cgacccgggg aacgccggcc agctgtgggg gaagctcgac
     1561 gccctcggcg gtcaacagca gcgccggtgc gctgtcggcg acgatgtggg ccagctcgcg
     1621 ctcgcccgcc ttggggttga tcggcaccac cggtacgcca gcgaccaggg cgccgaccac
     1681 cgccgcgcag gtctccagag ccggcaccgc ccacacggcg acgcggggcc tggactggag
     1741 cagaccggca aggtgggtcg ccacgccggt cagctcccgg tacgtcagga cccgctgccc
     1801 gaagcggatc gcttcctggt cgctggggtc ggccagcgga gggagcaggg tgccgcctgc
     1861 cgtcgaggtg gtcgaagcgc tcatcgggtg cctcccgccc gtcgcgagac ctcgcgcccg
     1921 tcggccgcga cgccgatcat ggatgctgtc tgaaggctag accagccgag ggaccccatg
     1981 acaggagcag caccatcggg cggggcggac cggccggtcc gcctcagcgc cgccgagctg
     2041 atcgacgcgg tcgtcgatcc cggctcctgg cggagctggg acgagccggc ggtggcgccg
     2101 cacggcctcg atcccagcta cgccgccgac ctggccaggg cgcgggaacg gacgggcttg
     2161 gacgaggccg tcctcaccgg caccggccgg ctacgcggcc gccaggtggc ggtggtcgtg
     2221 tccgaattcg ggtttctcgc cggttcgatc ggcgtcgccg ccggcgagcg gctggtccgc
     2281 gcagtcgagc gggcgggcag ggagcgcctg ccgctggtcg cggcgccggc ctcgggcggg
     2341 acccgcctgc aggagggcac ggtcgcgttc ctgcagatgg tcaaggtggc ggcggcgatc
     2401 gccgaccacc gcagggccgg gctgccctac ctggtctacc ttcggcaccc caccaccggg
     2461 ggcgtgctcg cctcctgggg gtcgctcggg catgtgaccg cggccgagcc gggggcgctg
     2521 atcggtttcc tggggccgcg ggtctatgag gccctgcacg accagccgtt cccgcccgac
     2581 gtgcaggtgg ccgagaacct ctacgccaag ggcctggtcg acgcggtggt ggcgcctggg
     2641 gaggtcgccg aggtggctgc ccgcgtcctg gatgtgctgt gcgccccccg cgaggtccca
     2701 caggccgccg agccgcccgc cgacgaccag gcgggcgaga ccccggtcgc tgagtcgatc
     2761 cgccgctcgc ggcgcccgga ccggccgggg gtgcgggccc tgctcagggt ggccgcgcgg
     2821 aacgtgacgc ccctgagcgg gaccgccgcc ggggagatgg accccggggt gctcctggtc
     2881 cttgcccgct tcggcgccgt cccctgcatc ctcctggggc aggaccggca ccggcagcgg
     2941 cgccagcagc cgctgggacc agccgggctg cgcgcggccc ggcgtggcat ccgcatcgcc
     3001 accgaactgg acctgccgtt ggtgacgatc gtggacacgg ccggggcgga cctgtccaaa
     3061 gaggccgagg agggcgggct ggccggggag atcgcccggt gcctggccga cctggtgacc
     3121 ctgccggccc cgaccctgtg cctgctcctc ggcgaggggg ctggcggcgg cgcgcttgcg
     3181 ttcctgccgg ccgaccgcgt cgtcgcggcc gagcacgcct ggctctcccc gctaccgccc
     3241 gagggggcat cggctgtcct gtaccgcacc accgagcggg cggtcgaggt cgccgagcgg
     3301 caaggcatcg gcgtggccgc ccttcgtgcc catcgcatcg tcgaccgggt ggtcccggag
     3361 cttcccgacg ccgccgacga gggtgaggcg ttcctgcaac gggtggg
//
LOCUS       DASSWR010000652         8170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_702993,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000652 DASSWR010000000
VERSION     DASSWR010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702993"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFO47_10900"
     CDS             complement(<1..557)
                     /locus_tag="VFO47_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET8789241.1"
                     /translation="MAQTGLLTLDELRAEVEAGRVDTVLLAMADMQGRLQGKRLAATH
                     FLDDVLHHNAEGCNYLLAVDVDMNTVSGYAMSSWDKGYGDFVFKPDLSTLRMVPWQEG
                     TALVMCDLEWEDGSPVLASPRGVLKRQLERLAERGWVAYAGTELEFIVFRDSYEEAWA
                     KAYRDLRPANDYNVDYSLLGTARIEP"
     gene            660..1646
                     /locus_tag="VFO47_10905"
     CDS             660..1646
                     /locus_tag="VFO47_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789242.1"
                     /translation="MPASTELSEAVLSQRRLLERLLVHGRRETGEEPSLRSPHAGDAE
                     FVKNLQIFLRLVVDADALYLRSAHGLSGDPAQAAALEHREEELRDRITLPTEVPLTLE
                     LLLGRRFELERLLIELGDRTYLAGRLADLYDEAPGTVATWRSLYGDELPVLLPGGVTR
                     HPEAAQPVGADEVEATRERLARLMHAKESQAQAVKARWALKQRVTAPTILVLLLAVAA
                     FGVAVAGMVADDRALWSAAAAGAAGAALGGLIRLRDEVTLGTQVRQFWLFFCGQVLVG
                     AAAGVLTFLIDDGGLVSVAGGPAGVAAVAFAVGFSEAAFVGLLTRLGGSVTG"
     gene            complement(1643..2410)
                     /locus_tag="VFO47_10910"
     CDS             complement(1643..2410)
                     /locus_tag="VFO47_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET8789243.1"
                     /translation="MTTYDVNLRLWRGDQAGGELRDYTVACEDGEVVLDAIHRVQATQ
                     AGDLAVRWNCKAGKCGSCSAEVNGRPRLLCMTRLNIFERGEPITVTPLRTFPVVRDLV
                     TDVSFNFEMAKRVPAFAPRPREADGTYRMAQEDIDRVQEFHKCIECFLCQNVCHVIRD
                     HEENKPSFAGPRMFIRYAELEMHPLETGDRRELLRSQMGLGLCNITKCCTEVCPEHIN
                     ITDNAIIPLKERVADVRYDPITWLGSKIRRRREGDRP"
     gene            complement(2407..4212)
                     /locus_tag="VFO47_10915"
     CDS             complement(2407..4212)
                     /locus_tag="VFO47_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET8789244.1"
                     /translation="MAEHETHEFDVVVIGAGGAGLRAAIEAHDRGARTALVCKSLLGK
                     AHTVMAEGGIAAAMGNVYPDDNWRVHFRDTMRGGKMLNSWRMAQLHAQEAPDRVYELE
                     EWGALFDRTDDGRILQRDFGGHRFARLAHVGDRTGLEMIRTLQHRAVSQGVEVFMECT
                     VMRLLADGDRIAGAVAYWRESGRLVVFRAKAVVLATGGIGKAWKVTSNSWEYTGDGHA
                     LALWAGADLVDMEFVQFHPTGMVWPPSVRGILVTEGVRGDGGVLRNSEGKRFMFDYIP
                     EMFRAETAETEEEADRWYDDRQNNRRTPDLLPRDEVARAINTEVKEGRGSPHGGVFLD
                     IASRKPADYIQRRLPSMYHQFKELADVDITREPMEVGPTCHYVMGGVRVDPDTQAATA
                     PGLFAAGEAAGGMHGANRLGGNSLSDLLVFGLRAGRYAAEHAASAGDPPAVDPAQVEA
                     AEREALAPFERAGAGENPYTVQQDLQDCMQELVGIIRTEGELKSALERITALKERAAR
                     VGVEGHRQYNPGWHLALDLRSLLTVSECVALAALERRESRGGHTRDDYPRPDPELGKV
                     KMVVRAREGKVAVSREPLDDLPDDLRQLFEEKPRS"
     gene            complement(4217..4996)
                     /locus_tag="VFO47_10920"
     CDS             complement(4217..4996)
                     /locus_tag="VFO47_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789245.1"
                     /translation="MASRTVNGDRARIGARTLRRDRWWLAPLVTVAVLGGFVIYGTWV
                     AFANQDYYADPYLSPFYSPCLAASCEEPTWAIVGSWWPLSPALLVLPFPLGFRLTCYY
                     YRKAYYRSFWWAPPACAVPDARPRYTGETRFPLILQNIHRYFFYLALPFPVILAWDAL
                     RAFHFPDGWGIGLGTVILLVNAVFLALYTLSCHSCRHLCGGGLNSFSKAPARYWMWRQ
                     VSALNARHMLLAWVSLVFVALADLYVRLVATGVISDPRLVF"
     gene            complement(5028..5567)
                     /locus_tag="VFO47_10925"
     CDS             complement(5028..5567)
                     /locus_tag="VFO47_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789246.1"
                     /translation="MRRYLVVANQTLGGEELWAAIRERVAAGDCHFHLLVPATPAGQL
                     DPGSTAEVAEAGGPWEAGPEGLDRKAARELLEDELDDADIRERGEDLGRRLARQRMRY
                     ELDRMRELGASVSGEIGVADPVDAVRVVLHRWEFDEVILSTLPGRRSRWLAADLPTKI
                     RRGFKLPVTQVTGTTAATR"
     gene            5754..6653
                     /locus_tag="VFO47_10930"
     CDS             5754..6653
                     /locus_tag="VFO47_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8789247.1"
                     /translation="MAGDQGRAHEVAFVLGGGGVLGAHEVGMLQALAEAGVRPDLVLG
                     TSVGALNGVFVAAHRDPGAAVPELAAVWREGVAAEAFGGSLFGRVRTLARSGTHLHPN
                     EPLRDLLKALPVRNIEELALPFQCVAASIERAAAHWFTTGPIVPAVLASAAVPGLLPP
                     VRVGDEHFFDGGLVHSIPIGRALELGARTVYVLHVGRIERPLQVPSRPWEVGLVAFEI
                     ARRHRFAEDMAAVPPGVTVHVLPAGADAQAGVDLSQLRYRDTSRIDERIQRAYEASAT
                     YLAEASAGGPGGSGLAPRSTGTG"
     gene            6657..7727
                     /locus_tag="VFO47_10935"
     CDS             6657..7727
                     /locus_tag="VFO47_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET8789248.1"
                     /translation="MLPPRIVRRLVLAPLVLVITVGLLVLLPGLVILAAAASPLLPGR
                     WRALRLLWFALIWLAMESVALFACLALWVASGFGGRLSSEAYLDRHYALMRWWLAVVF
                     QVATRTFRLRIEIEEPELTPEERRARLTRPVIVLSRHAGPGDSFLLVHHLLTRYRRKP
                     RIVMKAALQYDPSLDVVINRLPNAFVPARRGAGELVIREIERLATGLGPTGALVIFPE
                     GGNFTPRRRLRAIRLLEERRLAEEAARARAMEHLLPPKPGGALAAIRAAPTADVIFVA
                     HTGLEHVITVGDVWRALPMEQVLKARWWRVPAAEVPTGHEAQVDWLYDWWARIDLWIG
                     DNRPTALPRQDRQVGVAVQPRS"
     gene            7793..>8170
                     /locus_tag="VFO47_10940"
     CDS             7793..>8170
                     /locus_tag="VFO47_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HET8789249.1"
                     /translation="MAPPTLRLAQDPDADALLARDPLALLMGMLLDQQFPLEWAFRAP
                     WRLAQRMERTSLDAAELADWDPDAFVKLMAGPPALHRYPASMAGRVQALCRIVVDEYG
                     GDAAAIWRTAATGKELFARLRALP"
BASE COUNT         1007 a         3021 c         2968 g         1174 t
ORIGIN      
        1 ggctcgatcc gggccgtgcc cagcagcgag tagtcgacgt tgtagtcgtt ggccgggcgc
       61 aggtcgcggt aggccttggc ccaggcctcc tcgtagctgt cgcggaacac gatgaactcg
      121 agctcggtgc cggcgtaggc gacccagccg cgctcggcca gccgctccag ctgccgcttc
      181 agcacccccc tgggcgaggc caggaccggg ctgccgtcct cccattccag gtcgcacatg
      241 accagggcgg tgccctcctg ccaggggacc atccgcaggg tcgacaggtc gggcttgaag
      301 acgaagtcgc cgtaaccctt gtcccaggag ctcatcgcgt acccggagac ggtgttcatg
      361 tcgacgtcga cggccagcag gtagttgcag ccctcggcgt tgtggtggag cacgtcgtcg
      421 aggaagtggg tggcggccag ccgcttgccc tggagccggc cctgcatgtc ggccatggcc
      481 agcagcaccg tgtcgacccg tccggcctcg acctcggccc gcagctcgtc gagggtcagg
      541 agccccgtct gcgccatcgc acaccccttt cgctcgctgg atcttgttgg caggcaagcc
      601 aggctgtcaa cgccggttta tgctgttcag ccgaccaata ccgaaggagg aggttgcccg
      661 tgccggcgag caccgagctc agcgaggccg tgctctccca gcggcggctg ctggagcggc
      721 tgctggtcca cgggcgccgc gagacgggcg aggagcccag cctgcgcagc ccccacgccg
      781 gcgacgccga gttcgtcaag aacctccaga tcttcctgcg gctggtggtc gacgccgacg
      841 ccctgtacct gcggtccgcc cacggcctca gcggggaccc ggcccaggcc gcggccctcg
      901 agcaccggga ggaggagctg cgcgaccgca tcaccctccc gaccgaggtc ccgctcaccc
      961 tcgagctgct gctcggccgc cgcttcgagc tggagcggct gctgatcgag ctgggcgacc
     1021 gtacctacct ggccggccgg ctggccgacc tctacgacga ggcgccgggc acggtcgcca
     1081 cctggcggtc gctgtacggc gacgagctgc cggtgctgct ccccggcggg gtcacccgcc
     1141 atcccgaggc ggcccagccg gtgggggccg acgaggtcga ggcgacccgc gagcggctgg
     1201 cccggctgat gcacgccaag gagtcgcagg cccaggccgt caaggcccgc tgggccctca
     1261 agcagcgggt cacggccccg accatcctgg tcctgctgct ggccgtggcc gccttcggcg
     1321 tggccgtggc cgggatggtg gccgacgacc gggcgctgtg gtcggcggcg gcggccgggg
     1381 ccgccggggc ggccctcggc ggcctcatcc gcctccgcga cgaggtcacc ctcggcaccc
     1441 aggtccgcca gttctggctg ttcttctgcg gccaggtgct ggtcggcgcc gccgccggcg
     1501 tgctcacgtt cctgatcgac gacggcggcc tggtcagcgt ggccggcggc ccagccgggg
     1561 tggccgcggt cgcgttcgcg gtcggcttct ccgaggccgc gttcgtcggc ctcctcaccc
     1621 gcctcggcgg ctcggtgacc ggctagggcc ggtcgccctc ccggcggcgc cggatcttgc
     1681 tgcccagcca ggtgatcggg tcgtagcgga cgtcggcgac ccgctccttg agggggatga
     1741 tggcgttgtc ggtgatgttg atgtgctcgg ggcagacctc ggtgcagcac ttggtgatgt
     1801 tgcacaggcc caggcccatc tgggagcgga gcagctcgcg ccggtcgccg gtctccagcg
     1861 ggtgcatctc cagctcggcg tagcggatga acatgcgcgg tccggcgaag ctgggcttgt
     1921 tctcctcgtg gtcgcggatg acgtggcaga cgttctggca caggaagcac tcgatgcact
     1981 tgtggaattc ctgcacccgg tcgatgtcct cctgggccat ccggtaggtg ccgtcggcct
     2041 cgcggggccg gggggcgaac gccggcaccc gcttggccat ctcgaagttg aaggacacgt
     2101 cggtgaccag gtcgcgcacg accgggaagg tgcgcagggg cgtgacggtg atcggctcgc
     2161 cccgctcgaa gatgttcagg cgggtcatgc agagcagccg cgggcggccg ttgacctcgg
     2221 ccgagcagga gccgcacttg ccggccttgc agttccagcg gacggccagg tcgccggcct
     2281 gggtcgcctg gacgcgatgg atggcgtcga gcacgacctc gccgtcctcg caggcgacgg
     2341 tgtagtcgcg cagctcgccg ccggcctggt cgccgcgcca caggcgcagg ttgacgtcat
     2401 aggtggtcat gaacggggct tctcctcgaa cagctgcctg aggtcgtcgg gcaggtcgtc
     2461 cagcggctcc cggctcacgg ccaccttgcc ctcccgggcc cggacgacca tcttgacctt
     2521 gcccagctcc gggtcgggcc tggggtagtc gtcgcgggtg tggccgccgc ggctctcgcg
     2581 gcgctccagc gcggccaagg ccacgcactc ggacaccgtg agcagcgagc gcaggtcgag
     2641 ggccaggtgc cagcccgggt tgtactgccg gtgcccctcg accccgaccc gggccgcccg
     2701 ctccttgagg gccgtgatcc gttccagggc gctcttcagc tcgccctcgg tgcggatgat
     2761 gccgaccagc tcctgcatgc agtcctgcag gtcctgctgg acggtgtagg ggttctcgcc
     2821 ggcgccggcc cgctcgaacg gggccagggc ctcgcgctcg gccgcctcga cctgggccgg
     2881 gtcgacggcc ggggggtcgc cggccgaggc cgcgtgctcg gcggcgtagc ggccggcccg
     2941 caggccgaag accagcaggt cggacaggga gttgccgccg aggcggttgg ccccgtgcat
     3001 gcccccggcg gcctcgccgg ccgcgaacag cccgggcgcg gtggccgcct gggtgtcggg
     3061 gtcgacccgg accccgccca tcacgtagtg gcaggtcggg ccgacctcca tcggctccct
     3121 ggtgatgtcg acgtcggcca gctccttgaa ctggtggtac atcgagggca ggcggcgctg
     3181 gatgtagtcg gccggcttgc gcgaggcgat gtccaggaag accccgccgt gggggctgcc
     3241 ccggccctcc ttgacctcgg tgttgatggc ccgggcgacc tcgtcgcggg ggagcaggtc
     3301 gggggtgcgg cggttgttct ggcggtcgtc gtaccagcgg tcggcctcct cctcggtctc
     3361 cgccgtctcc gcccggaaca tctcggggat gtagtcgaac atgaaccgct tgccctcgct
     3421 gttgcgcagc accccgccgt cgccgcgcac cccctcggtg accaggatgc cgcggaccga
     3481 cggcggccag accatgccgg tggggtggaa ctggacgaac tccatgtcga ccaggtcggc
     3541 cccggcccac agggccaggg cgtggccgtc gccggtgtac tcccaggagt tcgaggtgac
     3601 cttccaggcc ttgccgatgc cgccggtggc cagcaccacc gccttggccc ggaacaccac
     3661 cagccggccc gactcgcgcc agtaggcgac cgccccggcg atccggtcgc cgtcggcgag
     3721 caggcgcatc acggtgcact ccatgaacac ctcgacgccc tgggacacgg cccggtgctg
     3781 gagggtccgg atcatctcca ggccggtgcg gtcgccgacg tgggccaggc gggcgaagcg
     3841 gtggccgccg aagtcgcgct ggaggatgcg gccgtcgtcg gtgcggtcga acagcgcccc
     3901 ccactcctcc agctcgtaca cccggtcggg ggcctcctgg gcgtggagct gggccatccg
     3961 ccagctgttg agcatcttgc cgccgcgcat ggtgtcgcgg aagtgcaccc gccagttgtc
     4021 gtcggggtag acgttgccca tggcggcggc gatgccgccc tcggccatga ccgtgtgcgc
     4081 cttgcccagc agcgacttgc agacaagggc ggtgcgggct ccccggtcgt gggcctcgat
     4141 ggcggcccgc aggccggccc cgccggcccc gatgacgacg acgtcgaact cgtgggtctc
     4201 gtgctcggcc atggttctag aacaccaacc tcgggtcgct gatgacgccg gtcgccacca
     4261 gccggacgta caggtcggcc agggccacga agaccaggct gacccaggcc agcagcatgt
     4321 gccgggcgtt gagggccgac acctggcgcc acatccagta gcgggccggg gccttggaga
     4381 agctgttcag gccgccgccg cagaggtgcc ggcaggagtg gcagctgagc gtgtagaggg
     4441 ccaggaacac ggcgttgacc agcaggatca cggtgccgag gccgatgccc cacccgtcgg
     4501 ggaagtggaa cgcccgcagc gcgtcccagg ccaggatcac cgggaagggc agggccaggt
     4561 agaagaagta gcggtggatg ttctggagga tcagggggaa gcgggtctcg ccggtgtagc
     4621 gggggcgggc gtcggggacg gcgcaggccg gcggcgccca ccagaacgag cggtagtagg
     4681 ccttgcggta gtagtagcag gtgagccgga acccgagcgg gaacggcagc accagcaggg
     4741 cgggggacag cggccaccag ctgcccacga tcgcccaggt cggctcctcg cagctggcgg
     4801 ccagacacgg cgagtagaac ggcgacaggt acgggtcggc gtagtagtcc tggttcgcga
     4861 atgccaccca ggtgccgtag atgacgaagc cgccgagcac ggccaccgtg accagcggtg
     4921 ccagccacca ccggtccctg cgcagggttc gggcgccgat cctggcccgg tcgccgttga
     4981 ccgtcctgct cgccatgcgg ttccttcctc gtcgtcctgt tcgtcggtca gcgcgtcgcc
     5041 gcggtcgtcc cggtgacctg ggtcaccggg agcttgaacc cgcggcggat cttggtcggc
     5101 aggtcggcgg ccagccagcg ggaccgccgc ccgggcaggg tggacaggat cacctcgtcg
     5161 aactcccagc ggtgcagcac caccctaacc gcgtccaccg gatcggccac cccgatctcg
     5221 ccgctgacgc tggcccccag ctcccgcatc cggtcgagct cgtaccgcat ccgctgccgg
     5281 gccagccgcc gccccaggtc ctcgccccgc tcccggatgt cggcgtcgtc cagctcgtcc
     5341 tccagcagct cccgcgccgc cttccggtcc agcccctccg gccccgcctc ccacggccca
     5401 ccggcctcgg ccacctcggc ggtggagccc gggtcgagct ggcccgccgg cgtcgccggc
     5461 accagcaggt ggaagtggca gtccccggcc gcgacccgct cccggatcgc cgcccacagc
     5521 tcctcccccc cgagggtctg gttggcaacc accagatacc gccgcacgac acaccacctt
     5581 cccgagcggg acatgctccc tgcatcctat gacgggcagg ctgcgaccgg gagcctgccc
     5641 cctcgcccga cgttggccgc ccctccggcc aggtcgcccc agtccccgga cgtcacgagt
     5701 ggccgtcccg gctgggccgt tatgggatca tcgggccgac ggagggagga acggtggcgg
     5761 gggaccaggg ccgggcgcac gaggtcgcct tcgtgctcgg cgggggcggg gtgctcggcg
     5821 cccacgaggt cggcatgctc caggcgctgg ccgaggccgg ggtccggccc gacctggtgc
     5881 tgggcacctc ggtgggggcg ctcaacggcg tgttcgtggc cgcccaccgc gacccgggtg
     5941 ccgccgtgcc cgagctggcc gcggtgtggc gcgagggcgt ggccgccgag gcgttcgggg
     6001 gctcgctgtt cgggcgggtc cggaccctgg cccggtccgg cacccacctg cacccgaacg
     6061 agccgctgcg cgacctcctg aaggccctgc cggtgcgcaa catcgaggag ctggccctgc
     6121 ccttccagtg cgtggccgcc agcatcgagc gggccgccgc ccactggttc accaccgggc
     6181 cgatcgtccc ggcggtgctg gcctcggcgg ccgtgcccgg cctgctgccg ccggtccggg
     6241 tgggcgacga gcacttcttc gacggcggcc tggtccacag catccccatc ggccgcgccc
     6301 tcgagctggg cgcccggacc gtgtacgtgc tccacgtcgg ccgcatcgag cggcccctgc
     6361 aggtgccgtc ccgcccctgg gaggtcgggc tggtggcgtt cgagatcgcc cgccgccacc
     6421 ggttcgccga ggacatggcc gcggtcccgc cgggggtgac cgtccacgtc ctgcccgccg
     6481 gggccgacgc ccaggccggg gtcgacctgt cccagctccg ctaccgcgac acctcccgga
     6541 tcgacgagcg catccagcgg gcgtacgagg cctcggccac ctacctggcc gaggcctccg
     6601 ctgggggtcc tgggggctca ggcctggccc cccggtcgac ggggacgggg tgacgggtgc
     6661 tgccaccgcg gatcgtgcgc cggctcgtgc tggccccgct ggtgctggtc atcaccgtcg
     6721 ggctcctggt cctgctgccc ggcctggtca tcctggccgc ggccgcctcg ccgctgctgc
     6781 ccggccgctg gcgggcgctg cggctgctct ggttcgccct gatctggctg gccatggagt
     6841 cggtcgccct gttcgcctgc ctggccctct gggtggccag cggctttggt ggccgcctca
     6901 gctccgaggc ctacctggac cgccactacg ccctgatgcg gtggtggctg gcggtggtgt
     6961 tccaggtcgc gacccgtacc ttccggctcc gcatcgagat cgaggagccc gagctgaccc
     7021 ccgaggagcg ccgggcccgc ctgacccgcc cggtgatcgt gctcagccgc cacgccggcc
     7081 ccggcgactc gttcctgctc gttcaccacc tgctcacccg ctaccggcgc aagccccgga
     7141 tcgtgatgaa ggccgccctc cagtacgacc ccagcctgga cgtggtcatc aaccgcctgc
     7201 ccaacgcctt cgtcccggcc cggcgcggcg ccggcgagct ggtcatcagg gagatcgagc
     7261 ggctggcgac cgggctgggc ccgaccgggg ccctggtgat cttccccgag ggcggcaact
     7321 tcaccccgcg gcgccggcta cgggccatcc gcctcctgga ggagcgccgc ctggccgagg
     7381 aggcggccag ggccagggcc atggagcacc tgctcccgcc caagcccggc ggcgccctgg
     7441 ccgcgatccg ggccgccccc accgccgacg tgatcttcgt cgcccacacc ggcctcgagc
     7501 acgtcatcac cgtgggcgac gtctggcggg ccctgcccat ggagcaggtc ctcaaggccc
     7561 gctggtggcg ggtcccggcc gccgaggtcc cgaccggcca cgaggcccag gtcgactggc
     7621 tgtacgactg gtgggcccgg atcgacctgt ggatcggcga caaccgcccg accgccctgc
     7681 cccgccagga ccgccaggtc ggggtggccg tccagccccg gtcctgaccg gcccgccggt
     7741 gggcgagctg cggtagcctc ctggcggcgg agatctcacg ggaggacgac gcatggcacc
     7801 accgaccctg cggctcgccc aggacccgga cgccgacgcg ctgctggcca gggacccgct
     7861 ggccctgctg atgggcatgc tgctcgacca gcagttcccg ctcgagtggg cgttccgggc
     7921 gccctggcgc ctggcccagc ggatggagcg gacgtcgctg gacgcggccg agctcgccga
     7981 ctgggacccc gacgcgttcg tcaagctgat ggccgggccg ccggccctcc accgctaccc
     8041 ggcgtcgatg gccgggcggg tccaggcgct gtgccgcatc gtcgtcgacg agtacggcgg
     8101 cgacgccgcc gccatctggc ggaccgccgc caccggcaag gagctgttcg cccggctgcg
     8161 ggccctgccc
//
LOCUS       DASSWR010000653         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_81796,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000653 DASSWR010000000
VERSION     DASSWR010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81796"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..876
                     /locus_tag="VFO47_10945"
     CDS             97..876
                     /locus_tag="VFO47_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8789250.1"
                     /translation="MVWSSPGGRSRLRAYLLAEAGAIGLGYGVAVLRDGAPVSGRGWA
                     LVAAGAVAVAVPLWVKSRRELRQETDTRSAETLAIETDAKLRLIVGDVVTPIAEVVGR
                     VHQASGKGERESLRGQLEQLVVEAAASLCDGERARAVFFQLERGEMRAAAWFGRGDPP
                     TTVFTDHPDDRRGHEALLLVRFHDYVVIDDVRAEALPRGTEPRPGAGYRSFISVSVFC
                     GEQNLGMLSVDAPEPGAFDETDLNVMRAMAQLLGAGLVDRR"
     gene            920..1099
                     /locus_tag="VFO47_10950"
     CDS             920..1099
                     /locus_tag="VFO47_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789251.1"
                     /translation="MMRGQVVPEEARAWTAGLLLATDYFAHARREAVAKASAAIRGRL
                     HRARRASSEDRGTSR"
     gene            complement(1068..1790)
                     /locus_tag="VFO47_10955"
     CDS             complement(1068..1790)
                     /locus_tag="VFO47_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HET8789252.1"
                     /translation="MDTQRQWGQPPGGMGPPGPGWRPPRRRLPAGAIAAGVVLLVVLL
                     AVSSLGNLRWWPSLPNPFGAREVDRSQPVLLKAIEDLEVFKAATGNFQVVVDLEKGTG
                     RIPLLIKGERTLFVAGGSVDAEVDFSGIGEGAIKVSADGERVEVTLPRARLTQARIDP
                     AQSQVFSRERGLLDRLGSVLSDNPTSERELYQLAQARMQAAAGQSDLLARAEQNTRAM
                     LESMLRSLGYTDVTVTFRDPPS"
     gene            complement(1800..2390)
                     /locus_tag="VFO47_10960"
     CDS             complement(1800..2390)
                     /locus_tag="VFO47_10960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET8789253.1"
                     /translation="MDPSVFVHPLALCESDDVGPRTRIWAFAHVLDGARIGADCSVGG
                     HAFVEGGAVLGDGVTVKNGVLLWSGVTVEDEVFLGPGAVFTNDASPRAPYPKGPAGWR
                     ATLVRRGATVGANATVLSGLTIGPWAMVGAGGVVTGDVPGHAIVVGNPARQAGWACRC
                     GRTLPGELACAACGRSYRLVDGGTLEVRSSATGHGG"
     gene            complement(2494..2709)
                     /locus_tag="VFO47_10965"
     CDS             complement(2494..2709)
                     /locus_tag="VFO47_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789254.1"
                     /translation="MSTNVDGPRPSWIPGHARRHQRTTDLDVRWLPGCQCGWSFYSSV
                     RSLRIAHRTHGFHLDRVHLARTTSSAS"
     gene            3389..>3645
                     /locus_tag="VFO47_10970"
     CDS             3389..>3645
                     /locus_tag="VFO47_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HET8789255.1"
                     /translation="MELGEVIGRIFGRHKKLIAALVLAGLLAGFAIHLDDAPRYSASA
                     RLVLDTSDPEDQAQSGVIADTTRAIASGPSLVREALEKIGVR"
BASE COUNT          500 a         1237 c         1390 g          518 t
ORIGIN      
        1 tcgacctgtt cctgggggac tgaccggcca cactgtggcc gatgagtgtc gtgggcgtga
       61 cgtcgggcgt cggcgtgctt tgggatactt cgccccatgg tgtggagctc gccgggaggg
      121 cggagtcggc tgcgggccta cctgctggcc gaggcggggg cgattgggct cgggtacggg
      181 gtggcggtgc ttcgtgacgg ggcgccggtg tcggggcggg ggtgggcgct ggtggcggcc
      241 ggggcggtgg cggtggcggt gccgctgtgg gtcaagtccc ggcgggagct gcgccaggag
      301 accgacaccc gctcggccga gaccctggcc atcgagaccg acgccaagct gcggctgatc
      361 gtgggcgatg tggtcacccc gatcgccgag gtcgtggggc gggtccacca ggcctccggc
      421 aagggcgagc gcgagtcgct ccgcgggcag ctcgagcagc tggtggtgga ggcggcggcc
      481 agcctctgcg acggcgagcg ggcccgggcg gtgttcttcc agctggagcg gggagagatg
      541 cgggccgcgg cctggttcgg gcgcggcgac ccgccgacca cggtgttcac cgaccatccg
      601 gacgaccggc ggggccacga ggcgctgctg ctggtccggt tccacgacta cgtggtgatc
      661 gacgacgtcc gggccgaggc gctgccgcgg gggaccgagc cgcggcccgg ggccggctac
      721 cggtcgttca tcagcgtgtc ggtgttctgc ggcgagcaga acctgggcat gctgtcggtc
      781 gacgcgcccg agccgggcgc cttcgacgag accgacctca acgtcatgcg cgccatggcc
      841 cagctgctgg gagccggcct tgtcgaccgc cgctgaagct ggcaacctga accggcgcga
      901 ccgacgagga ggcggggcca tgatgcgggg ccaggtggtg cccgaggagg cccgtgcctg
      961 gacggccggg ctgctgctgg ccaccgacta cttcgcccac gcccgccggg aggccgtggc
     1021 caaggccagc gccgccatcc gcggccgcct gcaccgggcc cgccgggcta gctcggagga
     1081 tcgcggaacg tcacggtgac gtcggtgtag ccgagcgagc ggagcatgct ctccagcatc
     1141 gccctggtgt tctgctcggc ccgggccagc aggtcgctct ggccggccgc ggcctgcatc
     1201 ctggcctggg cgagctggta gagctcgcgc tcgctggtcg ggttgtcgga cagcacagag
     1261 ccgagccggt cgagcagccc ccgctcccgg gagaacacct ggctctgcgc cgggtcgatc
     1321 ctggcctggg tgagccgggc ccgcgggagg gtgacctcga cccgctcgcc gtcggccgac
     1381 accttgatcg cgccctcgcc gatgccggag aagtcgacct cggcgtcgac gctgccgccg
     1441 gccacgaaca aggtccgctc gcccttgatg agcagcggga tcctgccggt gcccttctcc
     1501 aggtcgacca ccacctggaa gttgccggtg gcggccttga acacctccag gtcctcgatg
     1561 gccttgagca gcaccggctg gctgcggtcg acctcgcggg ccccgaaggg gttgggcaac
     1621 gacggccacc agcgcaggtt cccgaggctg gagacggcca gcagcaccac cagcaggacc
     1681 acgccggcgg cgatggcccc ggcggggagg cgccggcgcg gcggccgcca cccaggcccg
     1741 ggcggtccca tgccgcccgg cggctggccc cactgccgct gggtgtccat gcccaccgcc
     1801 tacccgccgt gaccggttgc tgaacttcgt acctcgaggg taccgccatc gaccagccgg
     1861 taggagcgcc cgcaggcggc gcaggccagc tcgccgggca gggtgcggcc gcagcggcag
     1921 gcccagccgg cctggcgggc cgggttgccg accacaatgg cgtggcccgg cacgtccccg
     1981 gtgaccacgc cgccggcccc caccatggcc caggggccga tggtcagccc cgacaggacg
     2041 gtggcgttgg cgcccaccgt ggccccgcgc cggaccaggg tcgcgcgcca gccggccggg
     2101 cccttgggat agggggcgcg ggggctggcg tcgttggtga agaccgcccc gggcccgagg
     2161 aacacctcgt cctcgaccgt cacccccgac cacagcagca cgccgttctt gacggtgacg
     2221 ccgtcgccga gcaccgcccc gccctcgacg aaggcgtggc cgccgacgct gcagtcggcg
     2281 ccgatcctgg ccccgtccag cacgtgcgcg aacgcccaga tgcgggtgcg gggcccgacg
     2341 tcgtcgctct cgcagagcgc gaggggatgc acgaacaccg acgggtccat cccggcaggc
     2401 tactccacgg tctcgctgcg gccaccgccg tcccgagggc caggggaacc agtcggaacg
     2461 gcggtggctg cggcgagcac ggccgggccg tgctcaggat gcggagctcg tggtacgggc
     2521 caggtggacc cggtccaggt ggaacccgtg ggtccggtgg gcgatgcgca ggctgcggac
     2581 gctggagtag aagctccagc cgcattggca gcccggcagc cagcggacgt ccaggtcggt
     2641 ggtccgctgg tgccgccggg cgtgaccggg gatccaggac ggacgcgggc cgtcgacgtt
     2701 ggtggacatc ggatgcactc cccccttggc cgatcagccc ttgccccgcg tgcgggaacg
     2761 ggtggatcac agggcttcga cgagtgtcgt agggggcttc gggcaggacc agcgccaatt
     2821 gccgtaggac gccgtgcccg gaatagcgta ggtcctcccg agctgccggg accgtcctct
     2881 ggctgggcgt gggcgaggga acagcgccgg tgcgacgacg gcccccaagc ggttcggcag
     2941 aaaactgcgc tgattgcccg gccgcgtcct ctgctatttg tacaggctcc tttgctgtaa
     3001 tccagcatcc ccctgatgag ctacgtaggt tggcagtagc ccatctgggc cagccgagag
     3061 gttgcctgcc tgctggggct acggcaaccc gacgatggca cctacgtcga agtggggtta
     3121 gttccaccgg caaacgtctc ctaagatgca atcgcaagat gcaatcgcag cggatatgca
     3181 ggctaggcgc tgaccggccg acgatcgcga gcgggaggcc aggggtcttt gcagcaaggg
     3241 ggggcatggt cagaaggccg cctcccgtaa tcgagatcgt ttccgccagc gatctcctgc
     3301 gatatgcaat tcgtattgag gacgctccca gggggctgcg tgacggccgt gcgcgggccg
     3361 gactcgcggt gcaggaggta gggctccgtt ggaactcggt gaggtgatcg gtcggatctt
     3421 cggccggcac aagaagctaa ttgcggcact ggtattggcg gggcttctgg ctggcttcgc
     3481 gatccatttg gacgacgccc cgcggtactc ggcgtcggct cggctggtgc tggacacctc
     3541 cgacccggag gaccaggcgc agtcaggcgt gatcgcggac accacccggg ccatcgcgtc
     3601 cggcccttcg ctcgtccggg aggcgctgga gaagatcggg gtccg
//
LOCUS       DASSWR010000654         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_335998,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000654 DASSWR010000000
VERSION     DASSWR010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335998"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..914)
                     /gene="era"
                     /locus_tag="VFO47_10975"
     CDS             complement(33..914)
                     /gene="era"
                     /locus_tag="VFO47_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET8789256.1"
                     /translation="MKSGLVALVGRPNVGKSTLLNALLGQKLAIVSDKPQTTRSAIRG
                     VLHRPEGQAVLVDTPGLHKPRTLLSQRLNDLVRGTLAEVDLVLQLVDAAAGVGAGDRF
                     LAGELAKVDTPRMCVVNKVDAASRAKTAAALQAAADLADFAEIVPVSARKGTQLDLLV
                     ELILRHLPEGRPLYPEGHTSDEPEQHLVAELIREKALALVRDELPHSVAVLVEEMGPD
                     PEREDLLVIRAFLFVERASQKPIVLGKGGSVLREIGTRAREELEALFGTHVYLDLRVK
                     LAKEWQRDPRQLSRLGF"
     gene            complement(911..1297)
                     /gene="cdd"
                     /locus_tag="VFO47_10980"
     CDS             complement(911..1297)
                     /gene="cdd"
                     /locus_tag="VFO47_10980"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET8789257.1"
                     /translation="MVAAARAARDRAYAPYSGFRVGAALLTEQGDVITAANVENASYG
                     LAICAERSAVVAAVAAGSRSFLAVAVAGNGPDPVTPCGACRQVLREFPRGAELEVLCA
                     GESGDALVTTTLGALLPDSFGPEALP"
     gene            complement(1369..2676)
                     /locus_tag="VFO47_10985"
     CDS             complement(1369..2676)
                     /locus_tag="VFO47_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET8789258.1"
                     /translation="MSPLTATDIWSLLAVVALTAVAAFLAAAETGLTRMSRARAMHLH
                     EEQRRGSTQLLALVEQPARFLNLVLLLVLVVQFFATALFTSVTNRVIGGGLGVVVAAT
                     VMTIVTFICAEVAPKTYAVQHTDRAALFVAPFVYLLTRLPGLGPLTRLLIAIGNVVTP
                     GKGLKSGPFVSEDEIKAMVDEAERDDVIEEEEREMIHSVFEFGDTILREVMVPRPDMV
                     AVPLQTSLQEVLELILRSGFSRIPVYDGTIDEVVGLAYAKDVLRRLHDGQADKPLADI
                     LRQAPFMPESMRAAECLREMRRNKSHMVIVIDEYGGTSGLVTLEDLLEEIVGEIADEY
                     DREEPNVEPLPDGDYRVNARLGIDEVNELLDVELPSTEWDSIGGLLFNLVGGVPREGQ
                     EVEFQGLRLRAERVQGRRIGRVRIHRLSPPEEPEDITAQAHRL"
     gene            complement(2673..3215)
                     /gene="ybeY"
                     /locus_tag="VFO47_10990"
     CDS             complement(2673..3215)
                     /gene="ybeY"
                     /locus_tag="VFO47_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET8789259.1"
                     /translation="MSVFLANEQADYRVDEPALTRLARLVMDAEGVEESEISLLLVDR
                     SVMTDLNERYMGEDRPTDVLAFPIDGPSPASGPPEGPTGPRGFAADRLGDADDEDAAD
                     DEDDDDAPWLLGDIVLCPAYAAEQATRNGQSLAAELELLTVHGLLHLCGFDHAEPDEE
                     RAMQARTNELLARWRAGERP"
     gene            complement(3271..>3951)
                     /locus_tag="VFO47_10995"
     CDS             complement(3271..>3951)
                     /locus_tag="VFO47_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET8789260.1"
                     /translation="GPVTQEGRTCRIQYGTSSPHLRDNVVFLVRSLGGVAYWRTRPAA
                     GRKPGHAQGRDVHYRSDAYVLDIRLPAGIEPFRLARKREIYSRSGGGRPMRFIDRIEP
                     AGEAETVCIQVAAADSLYVTDDFLVTHNTLNDSFIVLDEAQNTTPEQMKMFLTRLGFN
                     SKAVVTGDITQIDLPSGQHSGLNVVREILTGIDDLAFVYLSSRDVVRHRIVQDIVEAY
                     RRYDEARS"
BASE COUNT          538 a         1397 c         1445 g          571 t
ORIGIN      
        1 atccgcaccc tccttgcttt cagaacacca ggtcagaacc cgaggcggga gagctggcgg
       61 gggtcgcgct gccactcctt ggccagcttg acccgcaggt cgaggtagac gtgggtgccg
      121 aacagggcct ccagctcctc ccgggccctg gtgccgatct cgcgcaggac cgacccgccc
      181 ttgccgagga cgatcggctt ctggctggcc cgctccacga acaggaacgc ccggatgacc
      241 agcaggtcct cgcgctcggg gtcggggccc atctcctcga ccagcacggc caccgagtgg
      301 ggcagctcgt cgcggaccag ggccagcgcc ttctcgcgga tcagctcggc gaccaggtgc
      361 tgctcgggct cgtcgctggt gtggccctcg gggtacaggg ggcggccctc ggggaggtgg
      421 cgcaggatca gctcgaccag caggtcgagc tgggtgccct tgcgggccga caccggcacg
      481 atctcggcga agtcggccag gtcggcggcc gcctggaggg cggcggcggt cttggcccgg
      541 gaggcggcgt ccaccttgtt gaccacgcac atcctgggcg tgtccacctt ggccagctcg
      601 ccggccagga agcggtcgcc ggccccgacc ccggcggccg cgtcgaccag ctggaggacc
      661 aggtcgacct cggccagcgt gccccggacc aggtcgttca gccgctggct gagcagggtg
      721 cggggcttgt ggagcccggg ggtgtcgacc agcacggcct ggccctcggg ccggtgcagc
      781 accccccgga tggccgaccg ggtggtctgg ggcttgtccg agacgatcgc cagcttctgg
      841 ccgagcaggg cgttgagcag ggtcgacttg ccgacgttgg gccgcccgac cagcgccacc
      901 agccccgact tcatgggagc gcttccggcc cgaaactgtc ggggagcagc gcccccaggg
      961 tggtggtgac gagcgcgtcg ccggactcgc cggcgcacag cacctccagc tcggcgcccc
     1021 tggggaactc gcgcaggacc tggcggcagg ccccgcaggg ggtgaccggg tcgggcccgt
     1081 tgccggcgac ggcgacggcc aggaagctgc gggacccggc ggccacggcc gccaccaccg
     1141 ccgaccgctc ggcgcagatg gccaggccgt agctggcgtt ctcgacgttg gcggcggtga
     1201 tgacgtcgcc ctgctcggtg agcagcgccg cccccacccg gaagcccgag tagggcgcgt
     1261 aggcgcggtc gcgggcggcc cgggcggcgg cgaccagctc ggcgggcgac ggccgggagg
     1321 tggggttcgg gggggatgcg gggggcggag ccccccgcag tctgcggttc acagccggtg
     1381 ggcctgggcg gtgatgtcct cgggctcctc cggcggcgac agccggtgga tccggacccg
     1441 gccgatgcgc cggccctgga cccgctcggc ccgcaggcgc agcccctgga actcgacctc
     1501 ctggccctcc cgcggcaccc cgccgaccag gttgaacagc aggccgccga tcgagtccca
     1561 ctcggtcgag ggcagctcca cgtcgagcag ctcgttgacc tcgtcgatgc cgaggcgggc
     1621 gttgacccgg tagtcgccgt cgggcagcgg ctcgacgttg ggctcctccc ggtcgtactc
     1681 gtcggcgatc tccccgacga tctcctccag caggtcctcc agggtgacca ggccggaggt
     1741 gccgccgtac tcgtcgatca cgatcaccat gtggctcttg ttccggcgca tctcgcgcag
     1801 gcactcggcg gcccgcatcg actcgggcat gaacggggcc tggcgcagga tgtcggccag
     1861 gggcttgtcg gcctggccgt cgtggaggcg gcggagcacg tccttggcgt aggccaggcc
     1921 gaccacctcg tcgatggtgc cgtcgtagac ggggatgcgc gagaagccgg agcgcaggat
     1981 cagctccagc acctcctgga gcgaggtctg gagggggacg gcgaccatgt ccgggcgggg
     2041 caccatgacc tcgcgcagga tggtgtcgcc gaactcgaag accgagtgga tcatctcccg
     2101 ctcttcctct tcgatgacgt cgtcgcgctc ggcctcgtcg accatcgcct tgatctcgtc
     2161 ctcggacacg aacggcccgc tcttcaggcc cttgccgggg gtgaccacgt tgccgatggc
     2221 gatcagcagc cgggtcagcg gccccagccc gggcaggcgg gtgaggaggt acacgaacgg
     2281 ggccacgaac agcgccgccc ggtcggtgtg ctggacggcg taggtcttgg gcgcgacctc
     2341 ggcgcagatg aaggtgacga tcgtcatgac ggtggccgcg accaccaccc cgagcccccc
     2401 gccgatgacc cggttcgtca ccgaggtgaa cagggcggtg gcgaagaact ggaccaccag
     2461 gaccagcagc aggaccaggt tgaggaagcg ggccggctgc tcgaccaggg ccagcagctg
     2521 ggtggagccg cggcgctgct cctcgtgcag gtgcatggcc ctggcccggc tcatgcgggt
     2581 caggccggtc tcggccgcgg ccaggaaggc ggccaccgcg gtcagggcga ccaccgccag
     2641 caggctccag atgtcggtgg cggtgagcgg gctcacggcc gctcaccggc gcgccagcgt
     2701 gccagcagct cgttggtgcg ggcctgcatg gcccgctcct cgtcgggttc ggcgtggtcg
     2761 aacccgcaca ggtggaggag gccgtgcacg gtcagcagct ccagctcggc ggccaggctc
     2821 tggccgttgc gggtggcctg ctcggccgcg tacgccgggc agagcacgat gtcgcccagc
     2881 agccagggcg cgtcgtcgtc atcctcgtcg tcggcggcgt cctcgtcgtc ggcgtcgccc
     2941 aggcggtcgg ccgcgaaccc gcggggcccg gtcggcccct cgggcggccc cgacgccggc
     3001 gagggcccgt cgatcgggaa cgccagcacg tcggtgggcc ggtcctcgcc catgtagcgc
     3061 tcgttcaggt cggtcatcac cgaccggtcc accagcagca atgagatctc ggactcctcg
     3121 accccttcgg cgtccatcac cagacgggcc agccgggtca gggccggctc gtccacccga
     3181 tagtcggcct gttcgttggc caggaacaca ctcaatcccg gcctcccggc acctgagggc
     3241 tcgggcgggg atgcggaggt tcggggccca ctaggacctg gcctcgtcgt agcgccggta
     3301 ggcctcgacg atgtcctgca cgatcctgtg cctgaccacg tccctgctcg aaaggtacac
     3361 gaacgccagg tcgtcgatcc cggtcaggat ctcccggacc acgttcagcc ccgagtgctg
     3421 ccctgacggc aggtcgatct gggtgatgtc ccccgtcacc accgccttgg agttgaaccc
     3481 cagccgggtc aggaacatct tcatctgctc tggcgtggtg ttctgcgcct cgtccagcac
     3541 gatgaacgag tcgttgaggg tgttgtgggt gaccaggaag tcgtcggtca cgtacagcga
     3601 gtcggccgcc gccacctgga tgcagaccgt ctccgcctcc cccgcgggct cgatccggtc
     3661 gatgaaccgc atcggccgcc ctcccccgga tctggagtag atctcgcgct tgcgcgccag
     3721 ccggaacggc tcgatacccg cgggcagccg gatgtcgagg acgtacgcgt ccgagcggta
     3781 gtgcacatct ctgccctggg catggccggg cttgcggccg gcggccgggc gggtccgcca
     3841 gtaggcgacc ccgccgaggg agcgcaccaa gaacaccacg ttgtcccgga ggtgcggcga
     3901 ggacgtcccg tactggatgc ggcaggttcg cccctcctgc gtcaccgggc c
//
LOCUS       DASSWR010000655         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1352865, whole genome shotgun sequence.
ACCESSION   DASSWR010000655 DASSWR010000000
VERSION     DASSWR010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352865"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="VFO47_11000"
     CDS             complement(<1..1231)
                     /locus_tag="VFO47_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8789261.1"
                     /translation="MTERLGAADRPLRIAIIGAGPAGLYAADSLLRKRDSSLTIDVFN
                     RFPTPFGLVRDGVAPDHQSIKAVARVLERILADPRVRYFGNVTYGVDIHHRELKRHYD
                     QLVYAVGAQADRRMSIPGEDLPNSHPAISFVGWYNGHPDYRELPVDLSVERAVVVGNG
                     NVAMDVSRILATSPDQLARTDIADHALERLRQSRIREVVVLGRRGPAQASFTTPELRE
                     LGRLEGVDAIVDPRDLELDAVSQADMEEDRTATANLRILREYAARTRHPGPRRILLRF
                     LASPVEIIGTDGRITALKAERNELVVDANGALRSRGTGQFETIETGLVLRSIGYRTVP
                     IEGVPFDPATSTINNVAGQIVHPNTGEVLHGEYVVGWAKRGPTGLIGNNNPDSAATVD
                     AMLADLPALQRLRDNHRD"
     gene            complement(1239..1670)
                     /locus_tag="VFO47_11005"
     CDS             complement(1239..1670)
                     /locus_tag="VFO47_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8789262.1"
                     /translation="MPKPHMEELDEDECRKLLAERHLGRLAIPDFGGPVIFPVNYVFD
                     QDLVIFRTDPGSKLDAATERESVAFEVDATDEATRTGWSVVIRGTLADITDPDHLERL
                     RGLPLYPWAPGDKSRYVRVRPRSVTGRRIRIPDDLPFTWWG"
     gene            complement(1928..>2421)
                     /locus_tag="VFO47_11010"
     CDS             complement(1928..>2421)
                     /locus_tag="VFO47_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET8789263.1"
                     /translation="MRALPAGPTVVDMLANAAFLLGLTLALAPGAAATTRRFAFQQAH
                     HNFYRAAQFGLDAELAWPLAPSGRPRILAAPDLVRHLLPTARQGLEDAGVLAEEAGEL
                     LSVIEARAASGHTGSAWQRLALASLEPKLGRERALAAMLEQYLEHQRTGQPVHTWPLP
                     QPR"
BASE COUNT          337 a          778 c          935 g          371 t
ORIGIN      
        1 ggtcacggtg gttgtcgcgg agtcgctgca gagcaggcag gtcagccagc atggcatcga
       61 cggtggcggc agagtcgggg ttgttgttgc cgatcagacc cgttgggccg cgcttggccc
      121 agccgacgac atattcgcca tgcagcactt cgccggtatt aggatggacg atctgcccgg
      181 cgacgttgtt gatcgtcgag gtcgcaggat cgaagggcac gccttcgatc ggcacggtcc
      241 ggtagccgat ggaacgcagc accaggccgg tctcgatggt ctcgaactgg ccggttcccc
      301 ttgaccggag cgcgccgttg gcgtccacga cgagctcgtt ccgctcggcc ttcaacgccg
      361 tgatccgccc gtcggtgccg atgatctcca ctggcgaggc gaggaagcgc aggagaatac
      421 ggcgaggtcc gggatgccgg gtgcgggcgg cgtactcgcg gagaattcgc aggttcgccg
      481 tggcggtgcg gtcctcctcc atgtcggcct ggctgaccgc gtcaagctcc aggtcccggg
      541 gatcgacgat cgcgtccacc ccttcgagcc gccccagctc cctgagctcg ggggtggtga
      601 aggacgcctg ggccgggccc cgccgcccca ggaccacgac ctcgcggatt ctgctctggc
      661 gcagcctctc cagggcgtgg tcggcgatgt cggtcctggc cagttggtcg ggggaggtgg
      721 ccaggatgcg ggacacgtcc atggccacgt tgccgttgcc gaccaccacc gcccgctcga
      781 ccgagaggtc gacgggcagc tcgcggtagt cgggatggcc gttgtaccag ccgacgaagg
      841 agatggcggg gtggctgttg ggcaggtcct cgccggggat gctcatgcgc cggtcggcct
      901 gggccccgac ggcgtagacg agctggtcgt agtggcgctt cagctcccga tggtggatgt
      961 caacgccgta ggtgacgttg ccgaagtacc gcacccgcgg gtcggccagg atccgctcca
     1021 gcacccgggc caccgccttg atcgactggt ggtcgggcgc gaccccgtcg cggaccagcc
     1081 cgaacggggt ggggaagcgg ttgaagacgt cgatggtcag ggaactgtcg cgcttgcgga
     1141 gcaggctgtc ggcggcatag agcccggctg gcccagcccc gatgatggcg atccgtagcg
     1201 gccgatctgc cgcgccaagc cgttctgtca tggcacggtc agccccacca ggtgaagggg
     1261 aggtcgtcgg ggattctgat gcggcggccg gtgaccgagc gcggccgcac gcggacgtac
     1321 cggctcttgt cgccgggggc ccacgggtac aggggcaggc cgcggagccg ctccaggtgg
     1381 tcagggtcgg tgatgtcggc cagggtcccc cggatgacca cgctccagcc ggtgcgggtg
     1441 gcctcgtcgg tggcgtcgac ctcgaaggcg accgactccc gctcagtggc ggcatccagc
     1501 ttggatccgg ggtcggttcg gaagatgacc aggtcctggt cgaacacgta gttgaccggg
     1561 aagatcaccg gcccgccgaa gtccgggatg gccaggcggc cgagatgccg ctcggccagc
     1621 agcttgcggc actcgtcctc gtccagttcc tccatgtggg gcttgggcat gctggcacct
     1681 ccttgatggc gagtcacagc cccagagggt agaaggtggc gcggcgtctg ggtcccggcg
     1741 aagggcccgc gccatctggg ccgttggacc ctgcggccgc cgtgaccgga gcctgacact
     1801 ggccgccagg ctcctcacgc tgaccgtggt cgcgaccggg caggcacggg cggaatcgag
     1861 ccgggaggga tcgcacatgg ccagtatgga aggtcaggtc gtgctcgtca ccggtggcgt
     1921 agccgggtta ccggggttgc gggaggggcc aggtgtggac gggctggccg gtccgctggt
     1981 gctccaggta ttgctcgagc atggcggcca gggcccgctc gcggcccagc ttcggctcca
     2041 gtgaggcaag ggccaggcgc tgccaggccg acccggtgtg gccgctggcc gccctggcct
     2101 cgatcaccga gaggagctcg cccgcctcct cagcgagcac gccggcgtcc tccaggccct
     2161 ggcgggcggt cgggaggagg tggcggacca ggtcgggggc ggccaggatc ctggggcgac
     2221 cgctcggggc caggggccag gccagctcgg cgtccagccc gaactgggcg gcccggtaga
     2281 agttgtggtg ggcctgctgg aaggcgaacc ggcgggtcgt ggcggccgcc ccgggggcca
     2341 gggccagggt caggccgagc aggaaggcgg cgttggccag catgtcgacc acggtcggcc
     2401 cggccggcag ggcccgcatc t
//
LOCUS       DASSWR010000656         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_81847,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000656 DASSWR010000000
VERSION     DASSWR010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81847"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFO47_11015"
     CDS             <1..473
                     /locus_tag="VFO47_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8789264.1"
                     /translation="ILGNQGQSNYGAAKAGVAAFTVICALELARYGIRVNAIAPGART
                     AMTEGAFGQLDFEGDFDPLDPANVAPVVVWLLSDAAAEVSGQVVGITGGLVELYEGWH
                     VVATEELPRRWTPEELAGAAPRLFGDRPTRAPWRPSSLRRIIDDARSRPPADDR"
     gene            632..874
                     /locus_tag="VFO47_11020"
     CDS             632..874
                     /locus_tag="VFO47_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET8789265.1"
                     /translation="MDDKQVLDRIGALVEEEHALERQATGEGLDEHEQDRLHEVEVQL
                     DQCWDLLRQRRARRNAGQDPDTAQVRPEGTVEGYLQ"
     gene            complement(1059..>2377)
                     /gene="lon"
                     /locus_tag="VFO47_11025"
     CDS             complement(1059..>2377)
                     /gene="lon"
                     /locus_tag="VFO47_11025"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET8789266.1"
                     /translation="GAIIALVGPPGVGKTSLGESVARAMGRRFVRVALGGVRDEAEIR
                     GHRRTYVGAQPGRIARAIKEAGTMNPVFLLDEVDKVGADWRGDPSSALLEVLDPAQNH
                     SFRDHYLEVELDLSDVLFIATANVVETIPGPLLDRMEVVRLDGYTEDEKLAIARDHLL
                     ARQLDQTGLRADEVIVTEAALRTVVGDYTREAGVRNLEREIGKLLRKAATRIASGEVA
                     TPLVVDEGDVRGYLGRQKFFFEAAERTAVPGVATGLAVTGTGGDVLFIEASVMDGEPG
                     LTLTGQLGDVMKESASIALSYVRAHAGELGIDGDVAGRRYHVHVPAGAVPKDGPSAGV
                     TMTTALASLLTGRPVRSEVGMTGEVTLQGRVLPIGGVKQKVLAAHRAGLKEVILPERN
                     GADLEDVPESVRAEMTFHLASEIDQVLIHALAPEGPEAAEVPTQAA"
BASE COUNT          320 a          876 c          874 g          307 t
ORIGIN      
        1 gcatcctcgg caaccagggc cagtccaact acggcgcggc caaggccggg gtggccgcct
       61 tcaccgtcat ctgcgccctc gagctcgccc gctacggcat ccgggtgaac gcgatcgccc
      121 caggggcgcg gacggcgatg accgaggggg ccttcggcca gctcgacttc gagggcgact
      181 tcgacccgct cgacccggcg aatgtcgccc cggtggtggt gtggctgctg tcggacgccg
      241 ccgccgaggt ctccggccag gtcgttggca tcaccggcgg cctggtcgag ctgtacgagg
      301 gctggcacgt ggtcgccacc gaggagcttc cccgccgctg gacccccgag gagctggccg
      361 gcgccgcccc ccggctgttc ggcgaccgcc ccacccgggc gccgtggcgg ccgtccagcc
      421 tgcgccggat catcgacgat gcgcggagtc ggcctccggc cgacgatcgg tagacgccga
      481 ccggcagaat cgccgctcgg ctagcgggcg gcgctgttcg catgccccga tcctcggcca
      541 tccggcccgg tgccacatca gcagatgcag gagggcgagc cccccggcat catcaccggg
      601 agccagccca gatccaccct cggaggagct catggacgac aagcaggtcc tcgaccgcat
      661 cggcgccctg gtcgaggagg agcacgccct cgaacgccag gccaccggcg aaggcctcga
      721 cgagcacgag caggaccgcc tccacgaggt cgaggtccag ctcgaccagt gctgggacct
      781 cctccgccag cgccgcgccc gccgcaacgc cggccaggac cccgacaccg cccaggtccg
      841 ccccgaaggc acggtcgagg gttacctgca gtaggcacga gccccggtca ccgcgcctga
      901 cggcgcgccg gcagacgctc gacgatccgc accgtctcgg ggagcgtgtc ccggtcgagc
      961 gcgaaggcca ccgcgtcccg cgcgagccgc cgggcctcct ccaggtccca gtgccaggta
     1021 cgcgcatggg gcccgaacgt cggcggctcg tgccggtgtc aggcggcctg ggtggggacc
     1081 tccgcggcct cgggaccctc gggggcgagg gcgtggatga ggacctggtc gatctcgctg
     1141 gccaggtgga aggtcatctc ggcccggacc gactcgggga cgtcctcgag gtcggcgccg
     1201 ttgcgctcgg ggaggatgac ctccttgagc ccggcgcggt gggcggccag gaccttctgc
     1261 ttgaccccgc cgatgggcag gacccgcccc tggagggtga cctcgccggt catgccgacc
     1321 tcggaccgga ccggacggcc ggtgaggagg ctggccaggg cggtggtcat ggtcacgccg
     1381 gccgaggggc cgtccttggg gacggcgccc gcgggcacgt ggacgtggta gcggcggccg
     1441 gccacgtcgc cgtcgatgcc gagctcgccg gcgtgggcgc ggacgtagga gagggcgatc
     1501 gaggccgact ccttcatcac gtcgccgagc tggccggtca gggtcagccc cggctcgccg
     1561 tccatcacgc tggcctcgat gaacagcacg tcgccgccgg tgccggtcac ggccaggccg
     1621 gtcgcgaccc cgggcacggc ggtccgctcg gccgcctcga agaagaactt ctgacggccc
     1681 aggtagccgc gcacgtcgcc ctcgtcgacg accagcgggg tggccacctc gccggaggcg
     1741 atcctggtcg ccgccttgcg cagcagcttg ccgatctcgc gctccaggtt gcggaccccg
     1801 gcctcgcgcg tgtagtcgcc gaccacggtg cgcagggccg cctcggtgac gatcacctcg
     1861 tcggcccgca ggccggtctg gtcgagctgg cgggccagca ggtggtcacg ggcgatggcc
     1921 agcttctcgt cctcggtgta gccgtccagg cgcacgacct ccatgcggtc cagcagcggc
     1981 cccgggatcg tctcgaccac gttggcggtg gcgatgaaca gcacatccga caggtcgagc
     2041 tcgacctcga ggtagtggtc ccggaaggag tggttctggg ccgggtcgag gacctcgagc
     2101 agggccgagg aggggtcgcc gcgccagtcg gcaccgacct tgtccacctc gtcgagcagg
     2161 aacaccgggt tcatggtccc ggcctccttg atggccctgg cgatgcggcc cggctgggcg
     2221 ccaacgtagg tccgccggtg gccgcggatc tcggcctcgt cgcggacgcc gccgagagcg
     2281 acgcggacga acctgcggcc catggcccgg gccaccgact cgccgaggga ggtcttgccg
     2341 acccccggcg ggccgaccag cgcgatgatg gcgccgg
//
LOCUS       DASSWR010000657         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_646990,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000657 DASSWR010000000
VERSION     DASSWR010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646990"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1700
                     /locus_tag="VFO47_11030"
     CDS             30..1700
                     /locus_tag="VFO47_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8789267.1"
                     /translation="MNDRTAPQAGLREWIGLAVLALACLLYSMDLTVLHLAVPALSAD
                     LQPSSAQLLWITDIYGFMVAGFLVTMGTLGDRIGRRRLLLIGAAAFGVLSILAALSTS
                     PGQLIAARALLGIAGATLAPSTLSLIFSLFQDPRQRSVAIGIWITAFSAGGAIGPVLG
                     GVLLERYWWGSVFLLALPVMALLLTLGPRVLPEYRDPNAGRLDLVSAALSLVAVLAVV
                     FGLKLVAQDGLGMLAVSAVLVGLVVGVVFVRRQLTLADPMIDLRLFRIQAFNASLATN
                     LLGIFIAVGYFLFVAQYLQLVLGLSPLEAGLWSLPSAVGFIVGSNLAPRILRRVRPAY
                     VIGTGLGLAAVGLAVLTRVGGSRDADLAILVGASLVVSLGLAPVFTATTDLIVGSAPP
                     ERAGAASGISETGAELGGAFGIAILGSIGAAVYRGVLADTMPAGIPAEAAAVARDTLG
                     GAMGVAAQLPGELGAALVDAAREAFVQGMQLTVALSAVVAAGGAVMATVLLRAVPAGA
                     EAEAAEQPAPEPVAVAQPEPERAIQVIRVGSGCLACPGERQPSDVQRA"
     gene            1807..3147
                     /locus_tag="VFO47_11035"
     CDS             1807..3147
                     /locus_tag="VFO47_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8789268.1"
                     /translation="MTGSEVRYVNSGGVHIAYRVFGDGPLDLILVPAFTSHLEQNDEW
                     PGVVRWNARLVSFSRLIVFDKRSTGLSDHAPGPVVLEDTMQDVLAVLDAAGAERPAVC
                     GDLEGAALSALFAATHPERTRALVLYAPLVKGTGTGDFPWALAPELQGMFGEQLPAAW
                     GSDPMARLLAGVVAPSLADDQQFLQFLAKAMRSSVSPGAAQAWLRMIRDIDVREALPQ
                     IRVPTLIVHRTGDRAVDVGASRYAAGRIPGARLVELPDGDNLPFVGDTEAVAAEIEEF
                     LTGSRTPPMPDRVLATILFTDIAGSTERAAEVGDRRWRELLGRHDTLAREQLERYRGR
                     EVKMTGDGLLATFDGPARAVTCAGAIGREVRALGLEVRAAVHTGEVELRDGDIGGIAV
                     HIASRVMGLARPNEVLVSSTVKDLVAGAEIRFEDRSVQSLRGVPGQWQLFAAFT"
     gene            complement(3193..>3578)
                     /locus_tag="VFO47_11040"
     CDS             complement(3193..>3578)
                     /locus_tag="VFO47_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET8789269.1"
                     /translation="EHLLAHPAVVPFALQQPGLGPHSLRLGEAIYRVLRPAGFSDQAV
                     VGVVYALVTYILGFVALEIPRAGSDPQTSDEFVRRMWAFFAALPPGEFPHTVELAAQL
                     ARISTDDQFHFGIRTFLAGLNVEAT"
BASE COUNT          435 a         1217 c         1351 g          575 t
ORIGIN      
        1 gtctctcgac tcgttagcga aggatgctca tgaacgaccg caccgcacct caggccggac
       61 tccgggagtg gatcgggctc gccgtgctcg cgctggcctg cctgctctac tcgatggacc
      121 tgaccgtgct gcacctggcc gttccggccc tgagcgccga cctccaaccc agcagcgccc
      181 agctgctgtg gatcaccgac atctacgggt tcatggtcgc cgggttcctg gtcaccatgg
      241 ggaccctcgg cgaccggatc ggccgccgcc ggctgctgct gatcggcgcc gccgccttcg
      301 gcgtactctc gatccttgcc gccctgtcga ccagccctgg gcagctgatc gcggccaggg
      361 cgctgctggg gatcgccggg gcgaccctgg cgccgtccac cctgtcgctg atcttcagcc
      421 tgttccagga ccccaggcag cgctcggtcg ccatcgggat ctggatcacc gccttctcgg
      481 ccggcggcgc catcgggcct gtgctgggcg gggtactgct tgagcgctac tggtggggct
      541 cggtgttcct gctggcgctg ccggtgatgg cgctgctgct gacgctgggt ccgcgggtgc
      601 tgccggagta ccgcgacccc aatgccggcc ggctggacct ggtgagcgcg gcgttgtcgc
      661 tggtggcggt gctggcggtg gtcttcgggc tgaagctggt cgcccaggac gggctcggca
      721 tgctcgccgt ctcggccgtc ctggtggggc tggtggtggg cgtggtgttc gtgcgccgac
      781 agctgactct tgccgacccg atgatcgacc ttcggctgtt ccggatccag gcgttcaacg
      841 cgtcgttggc gaccaacctg ctcggcatct tcatcgcggt cgggtacttc ctgttcgtcg
      901 cccagtacct gcagctggtg ctcgggctct cgccgctgga ggcgggcctg tggtcgctgc
      961 catcggcagt cgggttcatc gtcggctcca acctggctcc ccggatcctg cgccgggtcc
     1021 gcccggccta cgtgatcggc accggcctgg ggttggcggc ggtcggcctg gccgtgctca
     1081 cccgggtcgg cggctcgcgc gacgccgacc tggcgatcct ggtgggtgcc tcgctggtgg
     1141 tgtcgctggg cctggcgccg gtgttcaccg ccaccaccga cctgatcgtc gggtcggccc
     1201 cgcccgagcg cgccggggcg gcctcgggga tctcagagac cggcgccgag ctgggcggcg
     1261 cgttcggcat cgcgatcctc ggcagcatcg gggcggcggt ctaccgcggc gtcctggccg
     1321 acacgatgcc ggcggggatc ccggccgagg ctgcggcggt cgcgcgcgac accctcggcg
     1381 gggcgatggg cgtggccgcg cagctgcccg gcgagctcgg cgcggcgctg gtcgacgcgg
     1441 cccgggaggc gttcgtgcag gggatgcagc tcaccgtcgc gctcagcgcc gtggtcgcgg
     1501 ccggcggcgc cgtcatggcg acggtcctgc ttcgggcggt gcccgccggt gccgaggccg
     1561 aggccgccga gcagcccgct cccgagccgg tcgccgtcgc ccagcccgag ccagagcgtg
     1621 ccatccaggt catccgggtg ggctcgggat gcctcgcatg cccgggcgag cgccagccct
     1681 cggacgtcca gcgcgcctag cggtcatccc cacaccaccg acctcgccgc tggatagaca
     1741 gccggccatc caggcgacac gatgcagctg gtgagtccgg cgaatggcgg cgttcgtggt
     1801 ggcccgatga ctggttccga ggttcggtat gtcaacagcg gcggcgtcca catcgcctat
     1861 cgggtgttcg gtgacggacc cctggacctg atcttggttc cagcgttcac ctcccacctg
     1921 gagcagaacg acgagtggcc gggtgtggtg cgctggaatg cgcggctggt gtcgttctcc
     1981 cggctcatcg tgttcgacaa gcgcagtacc gggctgtccg accacgcgcc aggaccggta
     2041 gtcctggagg acaccatgca ggatgttctg gcggtcctgg acgccgctgg ggccgagcgc
     2101 ccagccgtct gcggcgacct cgagggggcc gccctgagcg ccctgttcgc cgccacccat
     2161 cccgaacgca cgcgggccct ggtcctgtac gcgcccctgg tcaagggcac cggcaccggc
     2221 gacttcccct gggcgctggc gcccgagctg cagggcatgt tcggcgaaca gttgccggcc
     2281 gcctggggga gcgaccccat ggccaggctg ctggcgggcg tggtggcccc cagcctcgcc
     2341 gacgaccagc agttcctcca gttcttggcc aaggcgatgc ggagcagcgt cagtccggga
     2401 gctgcccagg cctggttgcg gatgatcagg gacatcgacg tgcgggaggc gctgccccag
     2461 atccgcgtgc cgacgttgat cgtgcatcgg accggcgacc gggccgtgga tgtgggggcg
     2521 agccgctatg ccgccggccg cattcccggc gccaggctcg tcgagctccc agacggcgac
     2581 aacctgccgt tcgtcggcga caccgaggcg gttgccgccg agatcgagga gttcctcacc
     2641 ggttcccgga ccccaccgat gccggatcgg gtcctggcca cgatcttgtt caccgacatc
     2701 gccggctcga ccgaacgcgc cgccgaggtg ggcgatcgcc gatggcggga gctgctcggg
     2761 cgccacgaca ccctggcccg agagcagctg gagcggtacc ggggccggga ggtgaagatg
     2821 accggcgacg ggctgctggc gaccttcgat ggcccggccc gggcggtgac gtgcgccggc
     2881 gcgatcggcc gggaggtgcg cgcgctcggc cttgaggtcc gggcggccgt gcacaccggc
     2941 gaggtcgagc tccgggacgg cgacatcggc gggatcgcgg tccacatcgc ctcgcgggtc
     3001 atgggtctgg cccggccgaa cgaggtgctg gtgtccagta cggtgaagga cctggtcgcg
     3061 ggagccgaga tccggttcga ggaccggagt gtccagtccc tgcggggggt gcccggccag
     3121 tggcagctgt tcgcggcgtt cacgtgaccc gtcctagctc aaccggggtt cctggcttgg
     3181 gctgcgaggc gttcaggttg cctcgacgtt gaggccggcc aggaaggtgc ggatgccgaa
     3241 gtggaactgg tcgtcggtgg agatgcgggc cagttgcgcg gccagctcga cggtgtgggg
     3301 gaactctccc ggtggcaggg cggcgaagaa ggcccacatg cggcggacga actcgtcgct
     3361 ggtctggggg tcggagccgg cccgggggat ctccagggcg acgaacccca ggatgtaggt
     3421 caccagggca tagacgaccc cgaccacggc ctggtcggag aacccggcgg gccgcaggac
     3481 acgatagatg gcctcgccga gccgcaggct gtgggggccg aggccgggtt gttggagggc
     3541 gaaggggaca acggcggggt gggcgagcag atgttcgc
//
LOCUS       DASSWR010000658         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_900639,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000658 DASSWR010000000
VERSION     DASSWR010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900639"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFO47_11045"
     CDS             <1..346
                     /locus_tag="VFO47_11045"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET8789270.1"
                     /translation="HDLEQDRFSMRLAFFRYLRPEARLYQLERRRGYLQERLADLRAS
                     VRTARERMDAYTIELMDHGLDEREREIAWLDRLIARERRSTEDAAEQDPAGPPAQLPQ
                     SPRDPRLPAQTG"
     gene            379..1455
                     /locus_tag="VFO47_11050"
     CDS             379..1455
                     /locus_tag="VFO47_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET8789271.1"
                     /translation="MGKLRVAIVGVGNCASSLVQGVQFYAAAEPGDHVPGLMHVELGG
                     YHVRDIEFVAAFDVDAKKVGRDLSEAIRSGENNTIQFADVPPLGVEVQRGPTLDGLGH
                     YYAETIQESDAAPVDVAVALAASGADVLVCYLPVGSEDAARFYAEQALKAGVAFVNCL
                     PVFIAGRPEWAERFTRAGVPIIGDDIKSQVGATIVHRVLSRLMEDRGIHIDRTYQLNF
                     GGNMDFMNMLERSRLESKKISKTQAVTSQVDHDLGKRNVHVGPSDYVPWLDDRKWCYI
                     RVEGRAFGDVPINLELKLEVWDSPNSAGVVIDAIRCAKLALDRGIGGPLLGPAAYFMK
                     SPPVQYTDDEARRMVEEFAARSEH"
     gene            1477..>2384
                     /locus_tag="VFO47_11055"
     CDS             1477..>2384
                     /locus_tag="VFO47_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET8789272.1"
                     /translation="MGAGDWWRRGVIYQIYPRSFADANGDGVGDLAGITARLDHLNDG
                     TPDSLGVDAIWLSPFYPSPMADFGYDVADYTDVDPVFGTLGDFDRLVAEAHARGIRVV
                     VDLVPNHTSDRHPWFLESRSSRDNPKRDWYVWADSRPDGSPPNNWMSSFPRAGKAWTF
                     DERTGQWYLHSFMPQQPDLNWWNPEVREAIDGVMRFWLDRGVDGFRVDVAHKMAHDPE
                     LLDNPLVELDDEDLGESQEARDRRRAKLKAVPRRDEDWPEVHEILRRFRRSLDAYHDR
                     MAVGEVYLLDLERLARYYGSGKDEFHL"
BASE COUNT          370 a          895 c          790 g          329 t
ORIGIN      
        1 ccacgacctg gagcaggacc ggttctccat gcgactggcc ttcttccgct acctgcggcc
       61 ggaggcccgc ctctaccagc tcgagcggcg gcgcggctac ctccaggagc ggctggccga
      121 cctgcgcgcc tcggtgcgga cagcccgcga gcgcatggac gcctacacca tcgagctgat
      181 ggaccacggg ctggacgagc gcgagcgcga gatcgcctgg ctcgaccgcc tgatcgcccg
      241 cgagcgtcgc tccaccgagg acgccgccga gcaggacccc gctgggcccc cggcccagct
      301 tccccagtcc ccgcgcgacc cccgcctgcc cgcacagacc gggtaggccg cgccgtctca
      361 ggaggagacc agacaccgat gggaaagcta cgcgtcgcga tcgtcggcgt gggcaactgc
      421 gcatcgtcac tggtgcaggg cgtgcagttc tacgccgcgg ccgagcccgg tgaccacgtc
      481 cccggtctga tgcacgtcga gctcggtggc taccacgtcc gcgacatcga gttcgtggcc
      541 gccttcgacg tagacgccaa gaaggtcggg cgcgacctct ccgaggccat ccgttccggc
      601 gagaacaaca ccatccagtt cgccgacgtg ccccccctcg gggtcgaggt gcagcgcggg
      661 ccgaccctcg acgggctcgg ccactactac gccgagacca tccaggagtc cgacgccgcc
      721 ccggtcgacg tggccgtcgc cctggccgcc tccggggccg acgtgctcgt ctgctacctg
      781 ccggtcggct ccgaggacgc cgcccgcttc tacgccgagc aggccctcaa ggccggcgtg
      841 gccttcgtga actgcctgcc ggtgttcatc gccggccgtc ccgagtgggc cgagcgcttc
      901 acccgggccg gggtgccgat catcggcgac gacatcaagt cgcaggtcgg cgccaccatc
      961 gtccaccggg tgctgagccg gctgatggag gaccgcggca tccacatcga ccgcacctac
     1021 cagctgaact tcggcggcaa catggacttc atgaacatgc tggagcgcag ccggctggag
     1081 tccaagaaga tctccaagac ccaggccgtc acctcccagg tcgaccacga cctgggcaag
     1141 cgcaacgtcc acgtcgggcc cagcgactac gtgccctggc tggacgaccg caagtggtgc
     1201 tacatccggg tcgagggccg cgccttcggc gacgtgccca tcaacctgga gctcaagctg
     1261 gaggtctggg actcccccaa ctcggccggc gtggtcatcg acgccatccg ctgcgccaag
     1321 ctcgccctcg accgcggcat cggcgggccg ctgctcggcc ccgccgccta cttcatgaag
     1381 tcgccgccgg tgcagtacac cgacgacgag gcccgccgca tggtcgagga gttcgcagcg
     1441 cggtctgagc actgagagac cagggaaccg gctggcgtgg gagcaggcga ctggtggcgg
     1501 cgcggggtca tctaccagat ctacccccgc agcttcgccg acgcgaacgg tgacggggtg
     1561 ggcgacctgg ccgggatcac ggccaggctc gaccacctga acgacggcac cccggactcg
     1621 ctcggggtcg acgccatctg gctgtcgccg ttctacccgt cgcccatggc cgacttcggc
     1681 tacgacgtcg ccgactacac cgacgtcgac ccggtgttcg gcaccctcgg cgacttcgac
     1741 cggctggtcg ccgaggccca cgcccgcggc atccgggtgg tggtcgacct ggtgcccaac
     1801 cacacctccg accgccaccc ctggttcctg gagtcgcgct cgtcccggga caaccccaag
     1861 cgcgactggt acgtctgggc cgactcccgg ccggacggct cgccgcccaa caactggatg
     1921 agctcgttcc cgcgggcggg gaaggcctgg accttcgacg agcgcaccgg ccagtggtac
     1981 ctgcactcgt tcatgcccca gcagcccgac ctcaactggt ggaacccgga ggtccgggag
     2041 gccatcgacg gggtcatgcg cttctggctg gaccggggcg tcgacggctt ccgggtcgac
     2101 gtcgcccaca agatggccca cgaccccgag ctgctcgaca atccgctggt cgagctggac
     2161 gacgaggacc tgggcgagag ccaggaggca cgggaccgcc ggcgggccaa gctgaaggcg
     2221 gtcccccgcc gggacgagga ctggcccgag gtccacgaga tcctgcgccg cttccggcgg
     2281 tccctggacg cctaccacga ccgcatggcc gtgggcgagg tctacctgct cgacctggag
     2341 cgcctggccc gctactacgg ctccggcaag gacgagttcc acct
//
LOCUS       DASSWR010000659         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1126156, whole genome shotgun sequence.
ACCESSION   DASSWR010000659 DASSWR010000000
VERSION     DASSWR010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126156"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..532
                     /locus_tag="VFO47_11060"
     CDS             203..532
                     /locus_tag="VFO47_11060"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET8789273.1"
                     /translation="MSEELASRHCVACKPGTPPIDEDRAVEHEAQLDPSWAREGTLRL
                     RRELEFPNFRDAFGFVARLALLAEAEGHHPDIELGWGRVAVSLTTHAAGGLTDNDFIL
                     AAKLDRL"
     gene            complement(540..1199)
                     /locus_tag="VFO47_11065"
     CDS             complement(540..1199)
                     /locus_tag="VFO47_11065"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET8789274.1"
                     /translation="MTVRHPRDELPGLLLGELPPGTAIDVDRHLAGCDPCRRDLAAVA
                     VASSALRDASRLPVMPAPDLPPMRLPPASPDPPLGRRRGRWLLGAAAVLLALLLGVGG
                     YTVGRDRDAGRGRTVALAAITGGASAEARMRGAGDDQIMTMTARGLPRLPAGAYYEVW
                     LVDDAGKEFPVGVLAANGEGIWSLPAEVTARYRVIDVTLEPADGDPSRSAESVLRGTY
                     A"
     gene            complement(1203..1736)
                     /locus_tag="VFO47_11070"
     CDS             complement(1203..1736)
                     /locus_tag="VFO47_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8789275.1"
                     /translation="MEQGASSNLGARLAAGEEGAINDVYSALGPMVLGYLRRFVPRDE
                     AEDVLQRVFYEVWRNRDRYDPARSLEAWVLAIARKRAIDQLRRRHANVVPIEELRDIA
                     GDDGRELAERYARANEVRGALERLPREQREALTLAYFGDLTQTEIAERLGVPLGTVKA
                     RTFRGLRRLAGILGTVG"
     gene            complement(1776..2321)
                     /locus_tag="VFO47_11075"
     CDS             complement(1776..2321)
                     /locus_tag="VFO47_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HET8789276.1"
                     /translation="MDWHRLGDGDSEAGRRAGVLDLLRRAQRELGGEPLTGTRFLYRA
                     RDMLAATREIEAAVRGADTTLFVGFQRAGKLDGEARTYQDLTAAGVRVVAFGTGEPER
                     LPGVEWVRLPEDQAALQNQWLLVTERPEPIVFVGFETSEPDRFGLLEVTDPARSFTGF
                     VSGDRRLVRAIAEYLEGISRP"
     gene            2458..>2860
                     /locus_tag="VFO47_11080"
     CDS             2458..>2860
                     /locus_tag="VFO47_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789277.1"
                     /translation="MSRTLPARPAVALAALAGMVLCLLVGLAGPAAAGPWVDRTAGQL
                     RDAPLYVNPSARPTLSLVDRERISARLALAGTPIFVAVLPAQALAEAGGSANDLASQV
                     ATSVGKPGTYLVVAGAEEGAGSNTLDPGAAAN"
BASE COUNT          360 a         1095 c         1060 g          345 t
ORIGIN      
        1 tgggccccag cagggcgacc cgctcgcccc ggcgcacggt caggtcgacg ccgtccacgg
       61 cccggccgcg gcggtacgtc ttgacaagtc cgcgggcgcg gaggacgtcg ctcatgccag
      121 ccagtgtccc agccgccccc aacatctcgc cacgggatat ccccagcttg tgcggcgccc
      181 ttggccggag gaggatgccg ccgtgagcga agagctggcc agccggcact gcgtggcctg
      241 caagccgggc acacccccga tcgacgagga ccgggcggtc gagcacgagg cccagctcga
      301 cccctcgtgg gcccgggagg gcacgctccg cctccggcgc gagctggagt tccccaactt
      361 ccgggacgcc ttcggcttcg tcgcccgcct ggccctgctg gccgaggccg aggggcatca
      421 ccccgacatc gagctcggct ggggccgggt cgcggtcagc ctcaccaccc acgccgccgg
      481 cggcctcacc gacaacgact tcatcctggc cgccaagctc gaccggctct gatcggcggt
      541 caggcgtagg tgccgcgcag gaccgactcg gcggagcggg acgggtcgcc gtcggccggc
      601 tccagggtga cgtcgatcac ccggtagcgg gcggtgacct ccgcgggcag gctccagatg
      661 ccttcgccgt tggcggcgag caccccgacc gggaactcct tgccggcgtc gtccaccagc
      721 cagacctcgt agtaggcgcc ggccgggagc cgcggcaggc cgcgggcggt catggtcatg
      781 atctggtcgt cgccggcgcc ccgcatgcgg gcctcggcgc tggcgccccc ggtgatggcg
      841 gccagggcga ccgtccggcc gcggccggcg tcgcggtcgc ggccgacggt gtagccgccg
      901 acgccgagca ggagggcgag caggacggcg gccgccccca gcagccagcg tccccggcgc
      961 cggcccaggg gcgggtccgg gctggcgggg ggcagccgca tggggggcag gtcgggcgcc
     1021 ggcatgacgg gcaggcggga ggcgtcgcgc agggccgagg aggcgacggc caccgcggcc
     1081 aggtcgcggc ggcacgggtc gcagccggcc aggtgccggt cgacgtcgat cgccgtaccc
     1141 ggcggcagct cgccgaggag caggccggga agctcgtccc gggggtggcg cacggtcacc
     1201 gctcagccca ccgtgccgag aatgccggcc aggcggcgca ggccccggaa ggtccgcgcc
     1261 ttgaccgtcc ccagcggcac cccgagccgc tcggcgatct cggtctgggt gaggtcgccg
     1321 aagtaggcga gggtgagggc ctcgcgctgc tcccgcggca ggcgctcgag cgcgccccgc
     1381 acctcgttgg cgcgggcgta gcgttcggcc agctcccggc cgtcgtcccc cgcgatgtcg
     1441 cgcagctcct cgatcggcac cacgttggca tgcctgcggc ggagctggtc gatggcccgc
     1501 ttgcgggcga tggcgagcac ccacgcctcg aggctgcggg cgggatcgta gcgatcccgg
     1561 ttccgccaga cctcgtagaa cacacgttgc agcacatcct cggcctcgtc gcggggcacg
     1621 aagcggcgca ggtagccgag caccatgggg ccgagggcgg agtagacatc gttgatcgcc
     1681 ccctcctcgc cggcggcgag acgggccccc aggttcgagc tggcgccctg ctccactcgt
     1741 ctccttcctt cgctcgaacc ggccggatgg tttcctcagg gtcggctgat cccttccagg
     1801 tactccgcga tggcccggac caatcgacgg tccccgctca cgaacccggt gaagctccgc
     1861 gccgggtcgg tgacctcgag caggccgaag cggtcgggct cggacgtctc gaagccgacg
     1921 aacacgatcg gctccggccg ctccgtgacc agcagccact ggttctggag ggccgcctgg
     1981 tcctcgggca ggcggaccca ctccaccccg ggcaggcgct ccggctcgcc ggtgccgaag
     2041 gcgaccaccc ggacccccgc cgccgtcagg tcctggtagg tgcgggcctc gccgtcgagc
     2101 ttcccggccc gctggaaccc cacgaacagg gtggtgtcgg cgccgcggac ggcggcctcg
     2161 atctcgcggg tcgcggccag catgtcgcgg gcccggtaca ggaaccgggt gccggtgagc
     2221 ggctccccgc ccagctcgcg ctgggcacgg cggagcaggt cgagcacgcc cgcccgcctg
     2281 cccgcctcgc tgtcgccgtc ccccagacgg tgccagtcca cgccacccac ctcgatcacc
     2341 ctgccggacg gcgccatgat ggccgcaacc accggtggcg cgcgaaccgg gtcgcggcca
     2401 cgcaccctcg acggaagcgg gacaatggtc ggagatcgcc cacgcaagcg aggtgagatg
     2461 tcccgcaccc tgccggcccg gccggccgtg gccctggcgg ccctggccgg gatggtgctg
     2521 tgcctgctgg tcggcctggc cggtccggcc gcggccggac cctgggtcga ccgcacggcc
     2581 gggcagctgc gcgacgcccc gctgtacgtg aacccgtcgg ccaggccgac cctgtcgctg
     2641 gtggaccggg agcggatctc ggcccggctc gccctcgccg gcacgcccat cttcgtggcc
     2701 gtcctgccgg cccaggcgct ggccgaggcc ggcggcagcg ccaacgacct ggcctcccag
     2761 gtcgcgacct cggtcggcaa gcctgggacc tacctggtgg tcgccggggc cgaggaggga
     2821 gcgggcagca acaccctcga cccgggcgcg gccgccaatc
//
LOCUS       DASSWR010000660         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_505106,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000660 DASSWR010000000
VERSION     DASSWR010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505106"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1489
                     /gene="prfB"
                     /locus_tag="VFO47_11085"
     CDS             join(382..450,452..1489)
                     /gene="prfB"
                     /locus_tag="VFO47_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187669.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET8789278.1"
                     /translation="MPTDHGPELEALRKRLGDAKEFLDLAGKERELAGLREQAAAPNL
                     WDDPERARRVMSRLAAVEGDLETVARLQGQLDDLETLNQLALEEDDDSVGGELTQGLA
                     SLGKAVEDLEVLALLGGEFDDRDAIVTIHPGAGGTESQDWAEMLWRMLVRWAERRGFK
                     VTVNDYQEGDEAGIKSVTVTLEGPRAYGLVSAEAGTHRLVRISPYDQAKRRHTSFAAV
                     DAIPVLEDEDTPEIDDADLRIDIYRSSGPGGQGVNTTDSAVRITHLPTGIVVAVQNER
                     SQLANKTIALKILAARLAERARKEREAQLAALRGDRGDIAFGSQIRSYVLHPYQMVKD
                     HRTNVETGNVQAVLDGDLDQFVAAELRRRATDRG"
     gene            1550..>2354
                     /locus_tag="VFO47_11090"
     CDS             1550..>2354
                     /locus_tag="VFO47_11090"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET8789279.1"
                     /translation="MRPRPGLRASTLLALPGAPAVAVGLLIAAYVAIFGWLTWAQHEN
                     FGTFGYDMGLYDQGIWLLSRFQDPFVTIRGLNYFEHHVNLITVLFVPAYWLGAGPQFL
                     YLVETVWMALGAVPLFLLARDRLRSGWLAVAVAGCFLLYPSLEWINWWHFHPDALVIT
                     PLLFAWWLATRRRWGWFAVAVGVALACKEDAALAVFMLGLVLAVRGERRAGLLTAAAG
                     AGWFLLATRVVIPAAGGGDGPFYQELFPGFGDSLGEIVWTMVVHPSRLLE"
BASE COUNT          310 a          833 c          888 g          323 t
ORIGIN      
        1 ggaggggtcg tggccgccct gggcgccggc cggcacggcc gcggccgcgg cggcctggct
       61 cgccggcgcg ggccggcggg cggtgacggc ccaggccgcg gtgccggcca gggccacgac
      121 gagcacggcg acgaccagcc acgggagacg ctgcttggac atggaacctc gctggggggc
      181 gcgagcggat ctggtgggga actcacctgg gtcgcgccgg attcccacga cttcccgggg
      241 agggcgaagg atgcgatccg gggaagccac caccagcgtt gccgagccga ggccggcggg
      301 cagcggcccg atggacgccg ccgcccgtcc gggtcggtac cattgccggg cgccgccgag
      361 ccttgaggag acccgacacc catgcccacc gatcacggac cggagctgga ggcgctgcgc
      421 aagcggctcg gcgacgcaaa ggagtttctt tgacctggcg ggcaaggaac gggagctggc
      481 cgggctgcgg gagcaggcgg ccgcgcccaa cctgtgggac gacccggagc gggcccggcg
      541 ggtgatgagc cggctggccg ccgtcgaggg tgacctcgag acggtggccc ggctccaggg
      601 ccagctcgac gacctcgaga cgctgaacca gctcgccctc gaggaggacg acgactcggt
      661 gggcggcgag ctcacccagg ggctggccag cctcggcaag gcggtcgagg acctcgaggt
      721 gctggccctg ctgggcgggg agttcgacga ccgggacgcg atcgtcacca tccacccggg
      781 ggccgggggg accgagtcgc aggactgggc cgagatgctc tggcggatgc tggtccggtg
      841 ggccgagcgg cgcgggttca aggtgacggt caacgactac caggagggcg acgaggccgg
      901 tatcaagtcg gtcaccgtga cccttgaggg gccgcgggcc tacgggctgg tgtcggccga
      961 ggccggcacc caccgcctgg tccgcatctc gccctacgac caggccaagc ggcggcacac
     1021 ctcgttcgcg gccgtcgacg ccatcccggt gctggaggac gaggacacgc ccgagatcga
     1081 tgacgccgac ctgcggatcg acatctaccg ctcgtcgggg ccgggcgggc agggggtcaa
     1141 caccaccgac tcggcggtgc gcatcaccca cctcccgacc gggatcgtgg tcgcggtcca
     1201 gaacgagcgc tcccagctcg ccaacaagac gatcgccctc aagatcctgg ccgcccggct
     1261 ggccgagcgg gcccgcaagg agcgggaggc ccagctggcc gccctgcgcg gcgaccgggg
     1321 cgacatcgcc ttcggcagcc agatccgctc ctacgtcctc cacccctacc agatggtgaa
     1381 ggaccaccgg acgaacgtgg agaccggcaa cgtccaggcg gtcctggacg gcgacctcga
     1441 ccagttcgtc gccgccgagc tccgccgccg cgccaccgac cgcgggtagc gccggtcagc
     1501 ccgggcggcc gggaggcccc ggctggtgcc gccctcgtag actacggccg tgcgacctcg
     1561 gcctggcctg cgcgcctcaa cgctgctcgc gctgccgggc gccccggcgg tggccgtcgg
     1621 gctgctgatc gccgcctatg tggccatctt cggctggctg acctgggccc agcacgagaa
     1681 cttcgggacc ttcggctacg acatgggcct gtacgaccag gggatctggc tgctgtcgcg
     1741 gttccaggac ccgttcgtga ccatccgggg gctgaactac ttcgagcacc acgtgaacct
     1801 gatcacggtg ctgttcgtgc ccgcctactg gctcggggcc ggcccccagt tcctctacct
     1861 ggtcgagacg gtctggatgg cgctgggggc ggtgccgctg ttcctgctgg cccgcgaccg
     1921 gctgcgcagc ggctggctgg ccgtggccgt ggccggctgc ttcctgctgt atccgtcgct
     1981 ggagtggatc aactggtggc acttccaccc ggacgcgctg gtgatcaccc cgctgctgtt
     2041 cgcctggtgg ctggccacga gacgccgctg gggctggttc gcggtcgcgg tgggggtcgc
     2101 gctggcctgc aaggaggacg ccgccctggc cgtcttcatg ctcggcctgg tcctggccgt
     2161 ccgcggcgag cgccgggccg ggctgctcac cgccgccgcc ggggccggct ggttcctgct
     2221 cgccacccgg gtggtgatcc cggccgccgg cggcggtgac ggccccttct accaggagtt
     2281 gttccctggc ttcggcgact cccttggcga gatcgtctgg accatggtcg tccacccgag
     2341 ccggctgctg gagg
//
LOCUS       DASSWR010000661         5435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_505309,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000661 DASSWR010000000
VERSION     DASSWR010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505309"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..612)
                     /locus_tag="VFO47_11095"
     CDS             complement(25..612)
                     /locus_tag="VFO47_11095"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET8789280.1"
                     /translation="MQPDERPPILPYCRVPVMVRESNAAAPAVARQVADLIAAKRPGA
                     VAEHVGSSAVPGLAGKGTVDLLLPTPPEEIPAVTDDLLELGFQRQQVATAFPPTRPML
                     QGVIRHGDTSFRVHVHVVPASSPEVTALRGFRDALLADPGLRAEYEELKRSIVGAGTV
                     DSVAFSKAKHAWIVAALERLGLTDAVEEVKQGGLP"
     gene            complement(646..1917)
                     /gene="purD"
                     /locus_tag="VFO47_11100"
     CDS             complement(646..1917)
                     /gene="purD"
                     /locus_tag="VFO47_11100"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET8789281.1"
                     /translation="MRIAVVGGGGREHALLATLAQSPDVERLFAVPGNAGTAAVAENV
                     AGLSAIDGPGLAAFAERNRVDLTIVGPEQPLVAGVADEFLDRGLPVFGPTAAAARIEG
                     SKAFAKEVMAAAGVPTARADSFTDPRAAVAALDDFGPPWVVKADGLAAGKGVTVTGDH
                     AAARAAVEAALVGRVHGDAGATILLEEFLEGPEASVFAVTDGRTVLPLAPARDHKRVG
                     DGDTGPNTGGMGAFSPLPDVDDDLVEEVRRTVLEPTVAELARRGARYQGLLYAGLTLT
                     ADGPRVLEFNCRFGDPETQVILPRMTSDLADLAWTAAEGTLSGCKVSWDPRACVTVVL
                     AAGGYPGSYRTGLEIGGLQEAAARPGVHLFHAGTALDQAGRVRTAGGRVLGVTALGDD
                     LADARARAYQAAGLVRFDGVHYRRDIAAVLP"
     gene            complement(1921..3201)
                     /locus_tag="VFO47_11105"
     CDS             complement(1921..3201)
                     /locus_tag="VFO47_11105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET8789282.1"
                     /translation="MPGTILVGSQWGDEGKGKATDLLADKMDVVVRYQGGNNAGHTVI
                     VGGRTFKLHLVPSGILYPHVTPVIGNGMVVDPGTLLAELAALDAQGIATGKLLISANA
                     HLIMPWHKELDKLTERWLGRQRIGTTGRGIGPTYADKVGRLGIRVQDLLDPGILAKKV
                     EAVLRERNPLLAKVYNRLPLDAEAIVEEYATYAEALRPRIADTSLYVWNALREGKQVL
                     FEGAQATLLDVDHGTYPFVTSSSPVAGGALAGAGVGPGWIDRVMGIAKAYVTRVGAGP
                     FPTEAGEAEAEVLRAAGDEFGTTTGRPRRCGWLDTVGLRYAARVNGLTELFVTKLDVL
                     SRLERIPVAVAYRIDGEVTEDWPMTQTEVHHAEPVYELFEGWREDISGVTRLEDLPRA
                     ARVYVDAVEKLGGVPVTAVGVGPGREQTLLRGEE"
     gene            complement(3290..4165)
                     /locus_tag="VFO47_11110"
     CDS             complement(3290..4165)
                     /locus_tag="VFO47_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8789283.1"
                     /translation="MKVASRTSITLWMVVGVLAISTSPILIKVAALPALAMAFWRCLA
                     GAAVLAPFASRGGLERLSRGDLSRLAASGVFLAAHFALWNASLGLTTVAAATTLVSCA
                     PLFVGLGSVFLGEPPSGRAWAGIVLATVGAVVIGAGDASGFGGGPDALLGDVLAFAGA
                     LAMAAYLLLGRVARQRLPVSTYAASVYGVAAATLLPACLLTGSSLAGYPAASWLALAG
                     VVAGPQLLGHTVFNGLLASVSASVVAVVLLLEPVLATLLAWWLFDELPGPALWAGGAM
                     VLAGVWLATTGSRRM"
     gene            complement(4162..4920)
                     /locus_tag="VFO47_11115"
     CDS             complement(4162..4920)
                     /locus_tag="VFO47_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HET8789284.1"
                     /translation="MPRTVGVLEAYPAAVAAGLDTVLGPALVGVYLHGSAALGGWEPS
                     RSDVDLLVVASRPLDRREKQVIGARLQHPSLHCPGGGLELSVVTAEVAADPPRLPPWE
                     LHATTGPEPRVVDGAGHPGDPDLVFYFAVCHRAGIAVAGPPPAEVFAEPPRPWLLERA
                     AEELRWAVAHDASFAYRVLTACRAWRFLDEDMLDSKIGAGEWARPRLPDPGLVDAALA
                     AQRALAPMPASAADIGAADRFVAAVLDRFAEARA"
     gene            complement(4965..5267)
                     /locus_tag="VFO47_11120"
     CDS             complement(4965..5267)
                     /locus_tag="VFO47_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET8789285.1"
                     /translation="MVCRAEQPGLGQVAREFEGTVQFFGVSYNDSVSEGRKYQRQFEV
                     PYPLANDPSGQSWAAYQVPYQPVTVVVDARGRVARRWNGAVEPEQLAPVLEYLVGA"
BASE COUNT          707 a         2021 c         2055 g          652 t
ORIGIN      
        1 ctggtgggcg cattctagaa cgttctaggg aaggcccccc tgtttgacct cctcgacggc
       61 gtcggtgagg ccgaggcgtt ccagggcggc gacgatccag gcgtgcttgg ccttggagaa
      121 ggcgaccgag tcgaccgtcc cggccccgac gatggaccgc ttcagctcct cgtactccgc
      181 gcgcaggccc gggtcggcca ggagggcgtc gcggaagccg cgcagggccg tcacctcggg
      241 gctggacgcc ggcaccacgt ggacgtggac ccggaacgag gtgtccccgt ggcggatgac
      301 gccctggagc atcggccggg tcggggggaa ggcggtggcc acctgctggc gctggaagcc
      361 gagctcgagc aggtcgtcgg tgaccgccgg gatctcctcg ggcggggtcg gcaggagcag
      421 gtcgacggtg cccttgccgg ccaggccggg cacggccgag ctgccgacgt gctcggccac
      481 ggcgccgggg cgcttggcgg cgatcaggtc ggcgacctgc ctggccaccg ccggcgcggc
      541 ggcgttggac tcgcggacca tgacggggac ccggcagtag ggcaggatcg gcggccgctc
      601 gtcaggttgc atatcaccca tctcgtacaa ccgtacgaag agtctctacg gtagcacggc
      661 ggcgatgtcc cgccggtagt ggacgccgtc gaaccggacc agcccggcgg cctggtaggc
      721 gcgggcccgg gcgtcggcca ggtcgtcgcc cagggcggtg accccgagca cccgcccgcc
      781 cgcggtgcgg accctcccgg cctggtcgag ggccgtgccg gcgtggaaca ggtggacgcc
      841 gggccgggcc gcggcctcct ggaggccccc gatctccagg ccggtgcggt aggagcccgg
      901 gtagccgccg gcggccagca ccacggtgac gcaggcccgc gggtcccagg agaccttgca
      961 ccccgacagc gtgccctcgg ccgccgtcca ggccaggtcg gccaggtcgc tggtcatgcg
     1021 gggcaggata acctgggtct cggggtcgcc gaagcggcag ttgaactcca gcaccctcgg
     1081 cccgtcggcg gtcagggtca gccccgcgta gagcaggccc tggtagcggg cgccccgccg
     1141 ggccagctcg gccacggtcg gctcgagcac ggtccggcgg acctcctcga ccaggtcgtc
     1201 gtcgacgtcg ggcaggggcg agaaggcgcc catgccgccc gtgttgggcc cggtgtcgcc
     1261 gtcgccgacc cgcttgtggt cgcgggccgg ggccaggggg agcacggtgc ggccgtcggt
     1321 gaccgcgaac acgctggcct ccggcccctc caggaactcc tcgagcagga tggtggcgcc
     1381 ggcgtcgccg tggacccgcc cgaccagggc ggcctcgacg gcggcgcggg cggccgcgtg
     1441 gtcaccggtg acggtgacgc cctttccggc ggccagcccg tcggccttga ccacccaggg
     1501 ggggccgaag tcgtcaaggg cggccaccgc cgcccggggg tcggtgaagc tgtcggcgcg
     1561 ggccgtcggc accccggcgg cggccatgac ctccttggcg aacgccttgg agccctcgat
     1621 gcgggcggcc gccgccgtcg gcccgaacac cggcaggccg cggtccagga actcgtcggc
     1681 cacgccggcg accaggggct gctcggggcc gacgatcgtc aggtcgaccc ggttccgctc
     1741 ggcgaaggcg gccagcccgg gcccgtcgat ggcgctcagc ccggcgacgt tctcggccac
     1801 cgccgccgtg ccggcgttgc cgggcacggc gaacagccgc tccacgtcgg gactctgggc
     1861 gagggtggcc agcagggcgt gctcgcgccc gcccccgccg acgaccgcga tccgcatctg
     1921 ttactcctct ccccgcagca aggtctgctc gcggccgggg ccgaccccga cggcggtcac
     1981 cgggaccccg cccagcttct cgacggcgtc gacgtacacc ctggccgccc gggggaggtc
     2041 ctccagccgg gtgacgccgc tgatgtcctc gcgccagccc tcgaacagct cgtagaccgg
     2101 ctcggcgtgg tggacctcgg tctgggtcat gggccagtcc tcggtgacct cgccgtcgat
     2161 ccggtaggcg acggccaccg ggatccgctc cagccgcgag agcacgtcca gcttggtcac
     2221 gaacagctcg gtcagcccgt tgacccgggc cgcgtagcgg agcccgacgg tgtcgagcca
     2281 gccgcagcgg cggggccggc cggtggtggt gccgaactcg tcgccggcgg cccgcagcac
     2341 ctcggcctcg gcctcgccgg cctcggtggg gaacgggccg gccccgaccc gggtcacgta
     2401 cgccttggcg atgcccatga cccggtcgat ccagccgggc ccgaccccgg ccccggccag
     2461 ggcgccgccg gccaccggcg aggacgaggt cacgaacggg taggtgccgt ggtcgacgtc
     2521 gagcagggtg gcctgggcgc cctcgaacag cacctgcttg ccttcgcgga gcgcgttcca
     2581 gacgtacagc gaggtgtcgg cgatccgggg ccgcagggcc tcggcgtagg tggcgtactc
     2641 ctcgacgatc gcctcggcgt ccagcggcag ccggttgtag accttggcca gcagcgggtt
     2701 gcgctcgcgc agcacggcct cgaccttctt ggccaggatc cccgggtcga gcaggtcctg
     2761 gacgcggatc ccgagccggc ccaccttgtc ggcgtaggtg gggccgatgc cccggccggt
     2821 ggtgccgatc cgctgccggc ccagccagcg ctcggtgagc ttgtccagct ccttgtgcca
     2881 gggcatgatc aggtgcgcgt tggccgagat gagcagcttg ccggtggcga tgccctgggc
     2941 gtcgagggcg gccagctcgg ccagcagggt ccccgggtcg accaccatgc cgttgccgat
     3001 cacgggggtg acgtgcgggt acaggatgcc gctggggacc aggtggagct tgaaggtgcg
     3061 gcccccgacg atcaccgtgt gcccggcgtt gttgccgccc tggtagcgga cgaccacatc
     3121 catcttgtcg gccagcaggt cggtcgcctt gcccttgccc tcgtctcccc actggctgcc
     3181 gacgaggatg gttcccggca tggtctgcgc tcgcttctcg aggtcggttg gtggtcgctg
     3241 ggggcccggg gggctcaagg ccggcccccg gtggttccct cgcagcagcc tacatccgtc
     3301 gcgagccggt cgttgccagc cacaccccgg ccaggaccat ggcgccgccg gcccacagcg
     3361 ccgggcccgg cagctcgtcg aacagccacc aggcgagcag ggtggcgagc accggctcca
     3421 gcagcaggac cacggccacc accgaggcgc tcacgctggc caggaggccg ttgaacacgg
     3481 tgtggccgag cagctgggga ccggccacca ccccggccag ggccagccag gaggcggccg
     3541 ggtagccggc caggctcgac ccggtgagca ggcaggccgg cagcagggtg gccgcggcaa
     3601 cgccgtagac cgaggcggcg taggtcgaga ccggcagccg ctgcctggcc acccggccca
     3661 gcagcaggta ggcggccatg gccagcgccc cggcgaacgc cagcacgtcc ccgaggagcg
     3721 cgtccggccc gcccccgaac ccgctggcgt ccccggcccc gatgaccacc gccccgacgg
     3781 tggccaggac gatcccggcc caggcgcggc cgctgggcgg ctcgccaagg aacaccgacc
     3841 cgaggccgac gaacagcggc gcgcagctga ccagcgtggt cgcggcggcc acggtggtga
     3901 ggcccaggga ggcgttccag agggcgaagt gcgcggccag gaacaccccc gaggcggcca
     3961 gccgggacag gtccccgcgg ctcaaccgct cgagtcctcc ccgtgaggcg aacggcgcca
     4021 gcacggccgc tccggccagg cagcgccaga acgccatggc cagcgcgggc agggccgcca
     4081 ccttgatcag gatcggcgag gtcgagatgg ccagcacccc gacgaccatc cacagggtga
     4141 tgctggtccg cgacgcgacc ttcacgcacg ggcctcggcg aaccggtcga ggacggcggc
     4201 cacgaaccgg tcggcggcgc cgatgtcggc cgcgctggcc ggcatcgggg ccagggcccg
     4261 ctgggcggcc agggcggcgt cgaccaggcc ggggtcgggc agcctcgggc gcgcccactc
     4321 gccggccccg atcttggagt cgagcatgtc ctcgtccagg aaccgccagg cccggcaggc
     4381 ggtgagcacc cggtaggcga aggaggcgtc gtgggccacc gcccagcgca gctcctccgc
     4441 ggcccgctcc agcagccagg gccggggcgg ctcggcgaac acctcggccg gcggcggccc
     4501 ggccacggcg atccccgccc ggtggcagac ggcgaagtag aagaccaggt cggggtcgcc
     4561 cggatggccg gccccgtcca ccaccctggg ctccggcccg gtggtcgcgt gcagctccca
     4621 cggcggcagc cggggcgggt cggcggccac ctcggccgtg accacgctga gctccaggcc
     4681 gcccccgggg cagtgcaggg acgggtgctg gaggcgggcg ccgatcacct gcttctcgcg
     4741 ccggtcgagg ggccggctgg ccaccaccag caggtcgacg tcgctgcggg acggctccca
     4801 gccgcccagg gcggccgacc cgtggaggta gaccccgacc agcgccggcc ccaggaccgt
     4861 gtcgagcccc gcggccaccg ccgccgggta ggcctcgagc accccgaccg tccgcggcac
     4921 cccctcgggg ttccccggac tcctccggcc ggtcccacgc tccactaggc gccgaccagg
     4981 tactccagca cgggggccag ctgctcgggc tcgaccgcgc cgttccagcg ccgggccacg
     5041 cggccccggg cgtccacgac cacggtgacg ggctggtacg gcacctggta ggcggcccag
     5101 ctctggccgc tggggtcgtt ggccagcggg tacgggacct cgaactggcg ctggtacttg
     5161 cggccctcgc tcacggagtc gttgtaggag acgccgaaga actggaccgt tccctcgaac
     5221 tcccgggcaa cctgcccgag gccgggctgc tcggcccggc agaccactca ccaggactca
     5281 aagaagttca ccaccaccgg ctggccccgg aaccctccca gggtgatcgt gttgccgtcg
     5341 aagtcggtga cctggatgtc gggcgcgggc tggcgctcgc cggccgggac cacgccccgg
     5401 gggacgggcc gggtcgtgtc gctggtcgac ggcgg
//
LOCUS       DASSWR010000662         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1014145, whole genome shotgun sequence.
ACCESSION   DASSWR010000662 DASSWR010000000
VERSION     DASSWR010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014145"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFO47_11125"
     CDS             complement(<1..507)
                     /locus_tag="VFO47_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET8789286.1"
                     /translation="MRTESLVLGGDLGGTSTRVLVVGTDGRVRGRSTAAAGNPVSHPD
                     GAAAAFGDALRAALAAVDPDRVEASVIGVAGVSALRTASVAARFGRVWADAGLRCDPG
                     YAADLEVAFAAGTPEPDGSVLVAGTGATAGAVTDRRLTRTADGHGWLLGDDGSGFWLG
                     REAVRATLR"
     gene            578..1375
                     /locus_tag="VFO47_11130"
     CDS             578..1375
                     /locus_tag="VFO47_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET8789287.1"
                     /translation="MVDARSAAGGTGAPGSAVSAQAYLDALAPIMARVSRQTGGPVQQ
                     AAELVVASLRAGGVVQAFGTGHSEALAMEFAGRAGGLVPTNRISLRDVVIFGGESPEV
                     LGDGLLERDPQVAHRLYDLAAVAAADVFVIGSNSGVNGAIVEFARLVKEHGHRLIAIT
                     SFTQTTGVDSRHPSGAKLIDHADVALDNGAPYGDAVLPLPGGGTACAVSSITAALLAQ
                     LVVAEVVRRLLEAGEAPPVYVSANVPGGYERNSTLEARYAGRIRRTA"
     gene            1406..2815
                     /gene="ngcE"
                     /locus_tag="VFO47_11135"
     CDS             1406..2815
                     /gene="ngcE"
                     /locus_tag="VFO47_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0015764 - N-acetylglucosamine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine/diacetylchitobiose ABC
                     transporter substrate-binding protein"
                     /protein_id="HET8789288.1"
                     /translation="MGTPNQYSRRLFLQRAVLVSVAAAGGGSLLTGCASGGGGGTESG
                     GGETSAENPFGVQADAPLDVVIFKGGYGDDYAKFHEELYKKKFPNAEIKHAGITDIRQ
                     QLQPRFNSGNPPDVIDNSGAEQLPLATLADTGQLADLTPLFDAESIDGGKIRDTLNPV
                     AIEAGDYGGKILVLNYALEVYGLWYDKTLFDQKGWQPAETWDDFLALAEEIKGDGIAP
                     MAHQGKYPYYIQQVTMDMAVKHGGVEVVKAIDSLEPNAWKHPSVKMAAEALLEIKSKG
                     YMLQGTEGLDHIQSQTRWNEHKAAFIPSGSWLENEQKKVAPEDFATTVAPTPLMDGSK
                     LPFQSTRVSAAEAFIVPAKAKNVPGGQEYLRIMLSKEGASKFTELTAAPTVVTGAGEG
                     LDLTPAAASASELVKAGGDNNWNYYFFVWYSPMRPKIETAFGELAAGRVTADEFCAAC
                     QKAADETAKDPQTKKRTRS"
     gene            2854..>3124
                     /locus_tag="VFO47_11140"
     CDS             2854..>3124
                     /locus_tag="VFO47_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8789289.1"
                     /translation="MRHGQYRFIVGFLVLPLALYAIFVISPFIQAFYYSLTNWTGVSP
                     TFEFVGLQNFQTLTQDSLFLGAVRNNLVLLVGMPLAVILLALFYAF"
BASE COUNT          489 a         1147 c         1073 g          415 t
ORIGIN      
        1 gcgcagggtg gcccggacgg cctcgcgtcc gagccagaat cccgaaccgt cgtcaccgag
       61 cagccagcca tggccgtccg cggtccgtgt caaccggcgg tcggtcacgg cgccggcggt
      121 cgccccggtg cccgcgacca ggacggagcc gtcgggctca ggggtgccgg ccgcgaacgc
      181 gacctcgagg tcggcggcgt acccggggtc gcagcgcagg ccggcgtccg cccagacccg
      241 cccgaaccgc gcggccaccg acgccgtccg cagcgccgac acaccggcca cgccgatcac
      301 cgacgcctcc acccggtcgg ggtcgaccgc ggccagcgcc gcgcgcagcg cgtcgccgaa
      361 cgcggccgcc gccccgtcgg ggtggctgac ggggttgccg gcggcggcgg tgctccggcc
      421 gcggacccgc ccgtcggtcc cgaccacgag gacccgcgtc gaggtccccc ccaggtcgcc
      481 gccgaggacc aacgactctg tgcgcatagg ggctccagtc agcgggaatg gacattgtcg
      541 cagcggcagg cagccgatgg acaggaggcc agcttcgatg gtggacgcgc gcagtgcggc
      601 cggcggcacc ggggcccctg ggagcgccgt cagcgcccag gcctacctcg atgccctcgc
      661 gccgatcatg gccagggtca gccggcagac cggggggccg gtccagcagg cggccgagct
      721 ggtggtggcg tcgctgcggg ccggcggcgt cgtccaggcg ttcgggaccg ggcactccga
      781 ggccctggcg atggagttcg ccggtcgcgc gggcgggctg gtgccgacca accggatctc
      841 cctgcgggac gtggtcatct tcggcggcga gtcgcccgag gtgctcggcg acggcctcct
      901 ggagcgcgac ccacaggtcg cccaccgtct gtacgacctc gccgcggtgg cggccgccga
      961 cgtgttcgtc atcggttcga actccggcgt caacggcgcc atcgtcgagt tcgcgcggct
     1021 ggtcaaggag cacgggcacc ggctgatcgc gatcacctcg ttcacccaga cgacgggcgt
     1081 ggactcccgg cacccgtccg gcgccaagct gatcgaccac gccgacgtcg ccctcgacaa
     1141 cggcgcgccc tacggcgacg ccgtgctccc cctgcccggc ggcggcaccg cctgcgcggt
     1201 gtcgtccatc acggcggccc tgctcgccca gctggtcgtc gccgaggtcg tccggcggct
     1261 gctcgaggcc ggcgaggcac cgcccgtcta tgtgtcagcc aacgtccctg gtgggtatga
     1321 acgcaacagc accttggaag cgcgatacgc cggacgcatc cgacgtactg cctgagtccc
     1381 ccgatctctc aaaggagaca ggtccatggg cacccccaac cagtactcgc ggcggctgtt
     1441 cctccagcgg gccgtcctgg tctcggtcgc cgccgccggc ggcggctccc tgctgaccgg
     1501 ctgcgccagc ggcggcggcg ggggcaccga gagcggcggc ggcgagacga gcgccgagaa
     1561 ccccttcggc gtgcaggccg acgcccccct cgacgtcgtc atcttcaagg gcggctacgg
     1621 cgacgactac gccaagttcc acgaggagct gtacaagaag aagttcccca acgccgagat
     1681 caagcacgcc ggcatcaccg acatccgcca gcagctccag ccccgcttca acagcggcaa
     1741 ccccccggac gtgatcgaca actcgggcgc cgagcagctg ccgctggcca cgctcgccga
     1801 caccggccag ctggccgacc tgaccccgct gttcgacgcc gagtcgatcg acggcggcaa
     1861 gatccgggac accctgaacc cggtggcgat cgaggccggc gactacggcg gcaagatcct
     1921 ggtcctgaac tacgccctcg aggtctacgg cctctggtac gacaagaccc tgttcgacca
     1981 gaagggctgg cagccggccg agacctggga cgacttcctc gccctggccg aggagatcaa
     2041 gggcgacggg atcgcgccca tggcccacca gggcaagtac ccgtactaca tccagcaggt
     2101 gacgatggac atggccgtca agcacggcgg cgtggaggtg gtcaaggcga tcgacagcct
     2161 ggagccgaat gcctggaagc acccgtcggt caagatggcc gccgaggccc tgctggagat
     2221 caagtccaag ggctacatgc tccagggcac cgaggggctg gaccacatcc agtcgcagac
     2281 ccgctggaac gagcacaagg cggccttcat cccctcgggc tcgtggctgg agaacgagca
     2341 gaagaaggtc gcgcccgagg acttcgccac cacggtcgcc ccgacccccc tgatggacgg
     2401 ttccaagctg ccgttccagt ccacccgggt cagcgcggcc gaggcgttca tcgtgcccgc
     2461 caaggccaag aacgtccccg gcggccagga gtacctccgg atcatgctgt cgaaggaggg
     2521 ggcgtcgaag ttcaccgagc tcaccgccgc cccgaccgtc gtcaccggcg ccggcgaggg
     2581 cctcgacctc accccggccg cggcgtcggc cagcgagctc gtcaaggccg gcggcgacaa
     2641 caactggaac tactacttct tcgtctggta ctcgccgatg cggccgaaga tcgagacggc
     2701 cttcggtgag ctggcggccg gccgcgtcac cgccgacgag ttctgcgccg cctgccagaa
     2761 ggcggccgac gagaccgcca aggacccgca gaccaagaag cgcacgcgca gctagccggc
     2821 gaggtcctcg acgcggcgcg gagaaggagt gcgatgcgac acggtcagta ccggttcatc
     2881 gtgggcttcc tggtcctgcc cctggcgttg tacgccatct tcgtgatctc gccgttcatc
     2941 caggccttct actactcgct gaccaactgg accggcgtgt caccgacgtt cgagttcgtc
     3001 gggctgcaga acttccagac gctgacgcag gactcgttgt tcctgggggc ggtgcggaac
     3061 aacctggtgc tgctggtggg catgccgctg gcggtgatcc tgctggccct gttctacgcc
     3121 ttcc
//
LOCUS       DASSWR010000663         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1210810, whole genome shotgun sequence.
ACCESSION   DASSWR010000663 DASSWR010000000
VERSION     DASSWR010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210810"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..686
                     /locus_tag="VFO47_11145"
     CDS             9..686
                     /locus_tag="VFO47_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET8789290.1"
                     /translation="MATKGLKYAVDIVFCIDVTGSMSPILDRVKDNALRFYDDVQKNL
                     TDKGKNVDELRVRVIAFRDFKADGAAALQESSFYSLPEEQTGFAEFVRGLVPEGGGDA
                     PESGLEAVALAINSPWTSSGDRRRQVIVVWTDSPAQPLAGEPAPAPYGDKIPRDFSAL
                     TDLWENEQGVMGANAKRLIVFAPDGPGWTDISGVWENVVHHPSKAGDGLSEVDYGTII
                     DSIANSV"
     gene            728..1756
                     /locus_tag="VFO47_11150"
     CDS             728..1756
                     /locus_tag="VFO47_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET8789291.1"
                     /translation="MPSVSYKFCTAKVAGEGEDADPILRVGPDLGLLGVFDGMGGAGG
                     RVYDTPDGRHTGAYIASRFARNVVERLMLELIKPEWNLDGTATAAELQRVLASSLAAR
                     LAELKAPETALRSKLVKALPTTMTLAVLQRTNPASYACHLFWAGDSRAYVVQPAAGLR
                     QLTTDDLRSGGDAMRNLTDDSVMSNCISADTEFHINHRQVELQTPFLLLCATDGCFAY
                     VRSPMHFEHLLLSTLEDARDAEAWRRALEAKVTAITGDDAALALLGLGADLGGFKKLF
                     KNRAAELQRRYIDPLEEVDGQVHRAEQELADLKTRRTDIAGELWGAYRHDYERYLAAP
                     EPAGEEVS"
     gene            1753..2829
                     /locus_tag="VFO47_11155"
     CDS             1753..2829
                     /locus_tag="VFO47_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET8789292.1"
                     /translation="MRAGDVINGYTILEDFRVVGAGLSKWTFAAKNGREYFIKEFLSP
                     TYPDEHAPGSAQTKMRKRRRCAAFERHHRRIQEALAAVSGLGGNLIVTLDFFRWGAKY
                     YKVTEKVEVAGLEPGDVAALTFQDQLVLLKTVAHSLRILHDRGIVHGDLKPSNVLIKR
                     TELGYTTKLIDFDNAYLAGEPPPPEEIVGTMNYYSPELVGYIQETGTTGAELTQKADI
                     FALGLIYTEYLTGAPPPFDAATHQYAGVAVRAGETLRLPPTTLPAPVVELVERMLLGD
                     PAARPSIAETHITLMGIRTPATTTTAVLAEPPPAAASTDPTRLKGRGLRAATGRASAP
                     APERRPTRRLVGKLLERRKDTGAP"
     gene            3030..3272
                     /locus_tag="VFO47_11160"
     CDS             3030..3272
                     /locus_tag="VFO47_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789293.1"
                     /translation="MRELPRRRLEPDRPDDGPYDVYDLFGLTPADIDAAYDGYERVDT
                     RPRVRVYGCCLPVALGMLLAFLATITLLGNLLLAAL"
BASE COUNT          576 a         1249 c         1159 g          487 t
ORIGIN      
        1 aggcgcccat ggcgaccaag ggactgaagt atgccgtcga tatcgtcttc tgcatcgatg
       61 tgaccgggag catgagcccg atcctggacc gggtgaagga caacgcgctg cgcttctacg
      121 acgacgtgca gaagaacctg accgacaagg gcaagaacgt tgacgagctg cgggtgcggg
      181 tgatcgcgtt ccgggacttc aaggccgatg gcgcggccgc gctgcaggag tcgtcgttct
      241 actcgctccc cgaggaacag accggcttcg ccgagttcgt gcgggggctg gtccccgagg
      301 gcggcggcga cgcccccgag tccgggctgg aggcggtggc cctggccatc aactccccgt
      361 ggacgagcag tggggatcgc cgccggcagg tgatcgtggt ctggaccgac tcgccggccc
      421 agccgctggc cggcgagccc gctccggcac cctatggcga caagatcccc agggacttca
      481 gcgccctcac cgatctgtgg gagaacgagc aaggcgtgat gggggcgaac gccaagcggc
      541 tgatcgtgtt cgctccggac ggcccgggct ggaccgacat ctccggggtc tgggagaacg
      601 tcgttcatca cccgtccaag gctggcgacg gcctgtcgga ggtcgactac ggcaccatca
      661 tcgactcgat cgccaactcg gtgtgagcgt cgactcatcc gcagcgacgc cgccggctgc
      721 ctccgccatg cccagcgtct cgtacaagtt ctgcaccgcc aaggtcgccg gcgagggtga
      781 ggacgccgac ccgatcctgc gggtcggtcc cgacctcggc ctcctgggcg tcttcgacgg
      841 gatgggcggg gccgggggcc gggtgtacga cacccctgat ggccggcaca ctggcgccta
      901 catcgcctcc cggttcgccc gtaacgtggt cgagcggttg atgctggagc tgatcaagcc
      961 cgagtggaac ctggacggca cggccaccgc cgccgagctg cagcgggtac tggcgtcctc
     1021 gctggcggcc cgactggccg agctgaaggc accggagact gccctgcggt ccaagctggt
     1081 caaggcgctg ccgaccacca tgacgctggc ggtcctacag cgcacgaacc cagcaagcta
     1141 tgcctgccat ctgttctggg ccggcgactc ccgcgcctac gtggtccagc cggcggcggg
     1201 gctgcgccag ctcacaaccg atgacctacg ttcgggtggg gacgcgatgc ggaacctgac
     1261 cgacgactcg gtgatgagca actgcatctc ggccgacacc gagttccaca tcaaccaccg
     1321 ccaggtcgag ctccagacgc cgttcctgct gctgtgcgcc actgacgggt gcttcgccta
     1381 tgtgcgctcg ccgatgcact tcgaacatct gctgctgtcc accctggagg atgccaggga
     1441 tgccgaggcc tggcggcggg ccctggaagc gaaggtgacc gccattaccg gcgacgacgc
     1501 ggccctggcc ctgctcggat tgggcgccga cctcgggggg ttcaagaagc tgttcaagaa
     1561 ccgggcggcc gagctccagc gccgctacat cgacccgctg gaggaggtgg acggccaggt
     1621 gcaccgggcc gagcaggagc tggcggatct caagacccgg cggaccgaca tcgccggtga
     1681 gctgtggggc gcctaccggc acgactacga gcgctacctg gccgcgcccg agccggccgg
     1741 tgaggaggtg tcgtgagggc gggcgacgtc atcaacggct acaccatcct cgaggacttc
     1801 cgggtggtcg gggccggact gagcaaatgg accttcgccg ccaagaacgg ccgggagtac
     1861 ttcatcaagg agttcctcag cccgacctac cccgatgagc atgcgccggg cagcgcccag
     1921 accaagatgc gcaagcgccg acgctgcgcg gccttcgaac gccaccaccg gcgcatccag
     1981 gaggccctgg cggccgtctc cgggctcggc gggaacctga tcgtcaccct ggacttcttc
     2041 cgctgggggg ccaagtacta caaggtcacc gagaaggtcg aggtcgccgg cctggaacca
     2101 ggcgacgtgg ctgcgctcac cttccaggac cagctggtcc tgctcaagac cgtcgcccat
     2161 agtctgcgga tcctgcacga ccggggcatc gtccacggcg acctcaagcc gagcaacgtc
     2221 ctcatcaagc ggaccgagct cggctacacg accaagctga tcgacttcga caacgcctat
     2281 ctggccggcg agccaccccc acccgaggag atcgtcggca ccatgaacta ctactcgccc
     2341 gaactggtcg gctacatcca ggagaccgga accactggcg ccgagctcac ccagaaggcc
     2401 gacatcttcg ccctcggcct catctacacc gaatacctca ccggcgcgcc gccgcccttt
     2461 gatgcggcca cccaccagta cgccggcgtc gcggttcggg ccggcgagac cctgcggctt
     2521 ccccccacca ccctccccgc ccccgtcgtc gagctggtcg agcggatgct cctgggcgac
     2581 cccgccgcca ggccctccat cgccgagacc cacatcaccc tgatgggcat ccgcaccccg
     2641 gccaccacca ccaccgcggt cctggccgag cctccacccg cggccgcgtc gacggacccc
     2701 acccgcctca agggcagggg gctgcgggcg gccaccgggc gggcatcggc gcccgcaccc
     2761 gagcgccggc caacccggcg gctggtcggc aagctgctgg agcgcagaaa ggataccggc
     2821 gccccatgag catcaaatgg cgctgcgccg tctgcgaggc gatgaacgac ggaggcgaca
     2881 cctgcacggc ctgcggcgcg agggtcaccc agaccgtcgt ccaagctcga ccagccgaga
     2941 cgtcaccggt gccggaagaa acggcggcgc cgcagagacc gcgggcgccg cagccagcgc
     3001 tgggagacga agccgcgacc gaggtccccg tgcgggagct cccacgccgc cggctcgagc
     3061 ccgaccggcc cgacgacggt ccctacgacg tctacgatct cttcggcctg accccagccg
     3121 acatcgatgc cgcctacgac ggctacgagc gggtcgacac acgaccacgg gtccgggtct
     3181 acggctgttg cctacccgtt gcgctcggga tgctgctcgc ctttctggcg accatcacgc
     3241 tcctcggcaa cctgctcctc gcagcgctgt agcgcggagg ccgggcagcc catacccgtg
     3301 gcggccagac cagctcgccg tgcgatcacc gacatgggtc gctgtcgctg gctcaggcga
     3361 gcgctccggg cggccctggc cattccagga acaccatggt ggcgtcgtcg cgtggcgagg
     3421 ttccctggtg ggccaggatg gcgtgggcga gccggcgcat cgtctcggcc g
//
LOCUS       DASSWR010000664         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1380802, whole genome shotgun sequence.
ACCESSION   DASSWR010000664 DASSWR010000000
VERSION     DASSWR010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380802"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..687)
                     /locus_tag="VFO47_11165"
     CDS             complement(118..687)
                     /locus_tag="VFO47_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET8789294.1"
                     /translation="MSIGTFTEAFDRPIDPVDRLLRRRVILLSGPIDADRANDVMARL
                     LYLEGEDDEAPIELRINSPGGEVLSGLAVIDTIAQLACPVATTCAGMAASMASVLLAS
                     GSKGRRRATANARVLIHQPWAGQFQGQATDLERAAEEILRQRARIDQLLADATGQPVD
                     RIHRDTERDTWFSAAEALEYGLVDEVVGR"
     gene            796..1164
                     /locus_tag="VFO47_11170"
     CDS             796..1164
                     /locus_tag="VFO47_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789295.1"
                     /translation="MASFHIIEGFVIVGGWALLFLFGIGLFITKRDANRVYWGLLTVL
                     QVLLGVQLLVGLVLLLSGGRQPLLHYLYGAVFPALVIGVCHVLTRGLEKPPYHMFFTI
                     GSFFVFGLTARALMTGLGIG"
     gene            1283..2017
                     /gene="coxB"
                     /locus_tag="VFO47_11175"
     CDS             1283..2017
                     /gene="coxB"
                     /locus_tag="VFO47_11175"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET8789296.1"
                     /translation="MQPSRRAPARWLAVTALVLAAAGCTPESVTEQGQAIYNLYSLFL
                     YIAAVVFVVVSGLVIWSVIRYRRRDDELPTQTHGNNRLELIWTAIPTVIVLVLFVFTI
                     QAQNKVLDSPGEADVNVTATAFQWSWRFTYEGTGAEVVGGPETIPELVVPVGQRVRVK
                     LVSVDVVHSFYVPQTLFKRQAIPGVTNEFDLTFEKTGLFHGQCTQFCGLQHTDMVFRV
                     RVVTQGEYQSWLAETTRRGAARQPGT"
     gene            2035..>3605
                     /gene="ctaD"
                     /locus_tag="VFO47_11180"
     CDS             2035..>3605
                     /gene="ctaD"
                     /locus_tag="VFO47_11180"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET8789297.1"
                     /translation="MATTELPRPALPEAHHEEPGGIVDYLTTVDHKKIGILYIFTAFG
                     IFLAGGVLALVVRWELAQPGLQAMGENTYNQVFTMHGTLMIFLFAAQVSTGLANYLTP
                     LQIGAADVAFPRANAMSYWLYLFGSLIVFSSFFVAGGPAAVAWTAYPPLSTEYFQGTG
                     MDLWIIGLSVVGIAGILGAVNLVTTIFRMRMPGMTMFRLPLFCWGVLVNQLLILFAFP
                     PLTVAFVLLFMERNFGARFFDVAAGGNQVLYQHVFWFFGHPEVYIIILPIFGIISEVI
                     PVFSRKPLFGYRAMVFAFFGIAALSFGVWAHHMFTTGVVYLPYFSIMSLLIAVPTGIK
                     VFNWIGTMWRGSITFSTAMLMALGFILVFVNGGITGVMIASPPIDFAVNDTYFIVSHF
                     HYIMVGGLLFGMFAGFYFWFPKFTGKLLSERLGRWQFWLFLVGFNLTFFPQYLVGLDG
                     MPRRIADYQIERWTSANLASTVGAFLTGVSVLLFLWNFVVSMRRGAHAGDDPWEGNSL
                     EWVTTSPPPHHNFHEL"
BASE COUNT          494 a         1295 c         1168 g          648 t
ORIGIN      
        1 gctcgacctc cgacaggtag gtgagcgaca ccccggcggc ctcggcgacc tcgcgcagcg
       61 tgcggccctg gtcgaggcgg cgccggcgca gcgcctcgcc aacggcctgg cgcagcctca
      121 cctgcccacg acctcgtcga ccaggccgta ctcgagcgcc tcggcggccg agaaccaggt
      181 gtcgcgctcg gtgtcgcggt ggatgcggtc gaccggctgg ccggtggcgt cggccaggag
      241 ctggtcgatg cgggcccgct gacgcaggat ctcctcggcg gcccgctcca ggtcggtggc
      301 ctggccctgg aactggccgg cccagggctg gtggatgagg acacgggcgt tggccgtggc
      361 ccgccggcgg cccttgctcc ccgaggccag cagcaccgag gccatcgagg cggccatgcc
      421 ggcgcaggtg gtggcgaccg ggcaggcgag ctgggcgatg gtgtcgatga cggccaggcc
      481 gctcaggacc tccccgccgg gcgagttgat ccgcagctcg atcggcgcct cgtcgtcctc
      541 gccctccagg tacagcagcc gggccatcac gtcgttggcc cggtcggcgt cgatcggccc
      601 cgacagcagg atgacccggc ggcgcagcag ccggtcgacc gggtcgatcg ggcggtcgaa
      661 cgcctcggtg aaggtcccga tgctcatggg agcaggctac cggaccgggc ggccgccgac
      721 gacccgttcc gccgagggcg aaccgggata ggatcggatc cggtcccccg atcaagccag
      781 ccgacggagc gcgccatggc cagcttccac atcatcgaag gcttcgtgat cgtgggcggc
      841 tgggccctgc tgttcctgtt cggcatcggc ctgttcatca ccaagcggga cgccaaccgg
      901 gtctactggg gcctgctgac cgtgctccag gtgctgctcg gcgtccagct cctggtcggg
      961 ctcgtcctgc tgctctccgg cggccgccag ccgctgctgc actacctgta cggcgcggtg
     1021 ttcccggccc tggtcatcgg ggtctgccac gtgctcaccc ggggcctgga gaagcccccg
     1081 taccacatgt tcttcaccat cgggtcgttc ttcgtgttcg ggctgacggc ccgggcgctg
     1141 atgaccgggc tggggatcgg ctgacccgtg ggcccgtgac tggctactct atcgttcaac
     1201 cttcctccgc cccgcccgat ttgcaaggat gcggctccgc cccctagata gaggcagcgt
     1261 ccactccgac ccgaggatgg ccatgcagcc cagtcgtcgc gctcccgccc gctggctcgc
     1321 ggtcacggcg ctcgtcctgg ccgccgccgg ctgcaccccc gagagcgtca ccgagcaggg
     1381 ccaggccatc tacaacctgt acagcctgtt cctgtacatc gccgccgtcg tgttcgtggt
     1441 cgtgtcgggg ctggtcatct ggtcggtgat ccgctaccgg cgccgcgacg acgagctgcc
     1501 cacccagacc cacggcaaca accggctgga gctgatctgg acggcgatcc ccacggtgat
     1561 cgtgctggtc ctgttcgtgt tcaccatcca ggcgcagaac aaggtcctgg acagccccgg
     1621 cgaggccgac gtcaacgtga ccgccaccgc cttccagtgg agctggcgct tcacctacga
     1681 aggcaccggc gccgaggtcg tcggcggccc cgagaccatc cccgagctgg tcgtgccggt
     1741 cggccagcgg gtccgggtca agctggtgtc ggtcgacgtg gtgcattcct tctacgtgcc
     1801 ccagaccctg ttcaagcgcc aggccatccc gggcgtgacc aacgagttcg acctcacctt
     1861 cgagaagacc ggcctgttcc acggccagtg cacccagttc tgcggcctgc agcacaccga
     1921 catggtgttc cgggtgcggg tggtcaccca gggcgagtac cagagctggc tggccgagac
     1981 gaccaggcgc ggcgcggcca ggcaaccggg aacctaagga gcgacgagcg acagatggcg
     2041 accaccgagc ttccccggcc ggcgctgccc gaggcccacc acgaggagcc gggcggcatc
     2101 gtcgactacc tgaccaccgt cgaccacaag aagatcggca tcctctacat cttcaccgcc
     2161 ttcgggatct tcctggccgg cggggtgctg gccctggtcg tccgctggga gctggcccag
     2221 ccagggctcc aggccatggg cgagaacacc tacaaccagg tgttcaccat gcacggcacc
     2281 ctgatgatct tcctgttcgc cgcccaggtg tccaccgggc tggccaacta cctgaccccg
     2341 ctgcagatcg gcgccgccga cgtcgccttc ccccgggcca acgccatgtc ctactggctg
     2401 tacctgttcg gcagcctgat cgtgttctcc agcttcttcg tggccggcgg cccggccgcc
     2461 gtcgcctgga ccgcctaccc gcccctgtcg accgagtact tccagggcac cgggatggac
     2521 ctgtggatca tcgggctgtc ggtggtcggc atcgccggca tcctgggcgc ggtcaacctg
     2581 gtcaccacca tcttccggat gcgcatgccg ggcatgacca tgttccggct gccgctgttc
     2641 tgctgggggg tgctggtcaa ccagctgctg atcctgttcg ccttcccgcc cctgacggtg
     2701 gcgttcgtgc tgctgttcat ggaacgcaac ttcggggcca ggttcttcga cgtcgccgcg
     2761 ggcggtaacc aggtcctcta ccagcacgtg ttctggttct tcggccaccc cgaggtgtac
     2821 atcatcatcc tgcccatctt cgggatcatc agcgaggtca tccccgtctt ctcccgcaag
     2881 ccgctgttcg gctaccgggc catggtgttc gccttcttcg gcatcgccgc cctgtcgttc
     2941 ggggtgtggg cccaccacat gttcaccacc ggcgtcgtct acctgcccta cttctcgatc
     3001 atgtcgctgc tgatcgcggt gcccaccggg atcaaggtgt tcaactggat cggcaccatg
     3061 tggcgggggt cgatcacctt ctcgacggcg atgctgatgg cgctggggtt catcctggtg
     3121 ttcgtgaacg gcggcatcac cggggtgatg atcgcctcgc cgccgatcga cttcgccgtc
     3181 aacgacacct acttcatcgt ctcccacttc cactacatca tggtcggcgg gctgctgttc
     3241 gggatgttcg ccggcttcta cttctggttc cccaagttca ccggcaagct gctgtcggag
     3301 cggctgggcc gctggcagtt ctggctcttc ctggtcggct tcaacctcac cttcttcccc
     3361 cagtacctgg tcggcctgga cggcatgccg cggcgcatcg ccgactacca gatcgagcgc
     3421 tggacctcgg ccaacctggc ctcgaccgtg ggggcgttcc tgaccggggt gtcggtgctg
     3481 ctgttcctct ggaacttcgt ggtctcgatg cggcgtggcg cacacgccgg cgacgatccc
     3541 tgggagggca acagcctgga gtgggtgacc acctccccgc caccccacca caacttccac
     3601 gagct
//
LOCUS       DASSWR010000665         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_82469,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000665 DASSWR010000000
VERSION     DASSWR010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82469"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..363)
                     /locus_tag="VFO47_11185"
     CDS             complement(67..363)
                     /locus_tag="VFO47_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HET8789298.1"
                     /translation="MSHDAMFLAGIAWLTVLLGVAVVSIGRARTTAERLVALDTAVLM
                     LIGLLGLVAAQSRRTYALDAALILALLSFVSTLAGARYLGDRRPFDQPDEEGPP"
     gene            complement(360..854)
                     /locus_tag="VFO47_11190"
     CDS             complement(360..854)
                     /locus_tag="VFO47_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HET8789299.1"
                     /translation="MSAGRLGWVVLLTGVYLLSLGSLDPLDAAEGLMLATALSLGLRG
                     RLEGPQGAGPSLADRVAAFPLLVGGLLADVLRGTWDVALRVVGLRPVECSGVVLVPIG
                     ERTHLGVAVTGLLAGLSPGSMLVEVDNERQAMLFHVIDASDPDAVRDHLDRFYQRYQR
                     RVFP"
     gene            complement(851..2215)
                     /locus_tag="VFO47_11195"
     CDS             complement(851..2215)
                     /locus_tag="VFO47_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET8789300.1"
                     /translation="MAPLALLWPAAAVAVLADGRRRATGWGAVAVLAVAVVFLAALAL
                     RVAADGPVEVVAGGWPADVGIRLRADALGVVMALVSVAVVLAALVHGVVSGMSPRLFP
                     ALMLFLSVGLTGLFLTGDVFSFYVFFELAMISAYALSALGGEGRMVGSALIFAVVNLL
                     GSFMFLIATAALYRATGTLEMIAVADRARGIDPNTAVVIAVLYFVAFGVKLGLFPFHF
                     WLPSVYASAPPAVAAVLAGALANIGAYGLLRFQAGLLPRELALGAGVVVGIGTVSIIY
                     GAVQAIGRRDPREVLAYSAIGHAGYVLVALGVGGPVALAAAALFTVVNALGKALLFLA
                     VDLRGPLVSGAFAIGAFSVAGVPPSAGFFGKASLFDAGVQASSAPTVLLLFVGGALTF
                     VYLFQIYQHIYWRPPQDHHRAPRGARVVVAALAVLVLAVGLWPEPLLALSDQAGDAVA
                     RMVP"
BASE COUNT          391 a          817 c          820 g          187 t
ORIGIN      
        1 tgccgtagac ccccagggtc atgacgacca gccccagcac gaccagcacg tcgacgatca
       61 ccgacctcat ggcggcccct cctcgtcggg ctggtcgaag gggcgccggt cgccgaggta
      121 ccgggccccg gccagggtgg acacgaacga cagcagggcc aggatgagcg cggcgtccag
      181 ggcgtaggtg cgccggctct gagcggcgac caggccgagc aggccgatca gcatcaggac
      241 ggcggtgtcc agggcgacca ggcgctcggc ggtggtccgg gcccgcccga tcgacaccac
      301 cgccacgccc agcagcaccg tcagccaggc gatcccggcg aggaacatcg cgtcgtggct
      361 cacgggaaca cccgccgctg gtagcgctgg tagaagcggt cgaggtggtc gcggacggcg
      421 tcggggtcgg aggcgtcgat gacgtggaac agcatcgcct ggcgctcgtt gtcgacctcg
      481 accagcatcg atcccgggga cagcccggcc agcaggccgg tgacggccac gccgaggtgg
      541 gtgcgctcac cgatcgggac cagcacgacg ccggagcatt cgaccgggcg cagacccacc
      601 acccgcaggg cgacgtccca ggtgcccctg aggacatcgg cgaggaggcc gccgacgagg
      661 agcgggaagg cggcgacgcg gtcggccagg gacgggcccg ccccctgcgg cccctcgagc
      721 cggccgcgaa gccccagcga cagcgcggtc gccagcatca ggccctcggc ggcgtccagc
      781 gggtccaggc ttccgagcga gagcaggtag acgccggtga gcagcaccac ccatcccagc
      841 cggccggcgc tcacgggacc atcctcgcca cggcgtcgcc ggcctggtcg ctgagggcga
      901 gcagcggctc cggccacagg ccgaccgcca gcaccaggac ggccagcgcg gccacgacca
      961 cccgggcccc tctgggagcg cgatggtggt cctggggggg ccgccagtag atgtgctggt
     1021 agatctggaa caggtagacg aaggtgaggg cgccgcccac gaacagcagc agcaccgtgg
     1081 gcgcgctgct ggcctggacg ccggcgtcga acagggaggc cttgccgaag aagccggccg
     1141 agggaggcac cccggcgacg ctgaacgcgc cgatggcgaa ggcccccgac acgagcggcc
     1201 cacgcaggtc gacggccagg aacagcagcg ccttgccgag cgcgttgacc accgtgaaca
     1261 gcgcggccgc ggccagcgcc accgggccgc cgacgccgag cgcgaccagg acgtagccgg
     1321 cgtggccgat ggccgagtag gccaggacct cgcgggggtc gcgccggccg atggcctgca
     1381 ccgccccgta gatgatgctg acggtgccga tcccgaccac gacgccggcg ccgagggcca
     1441 gctcccgagg cagcaggccg gcctggaagc ggagcaggcc gtaggcgccg atgttggcca
     1501 gcgccccggc cagcacggcg gccaccgccg ggggggcgct ggcgtagacg ctgggcagcc
     1561 agaagtggaa cgggaacagg cccagcttga ccccgaaggc gacgaagtac aggaccgcga
     1621 tgaccaccgc cgtgttgggg tcgatgcccc gggcccggtc ggccacggcg atcatctcca
     1681 gggtgccggt ggcccggtac agagccgcgg tggcgatcag gaacatgaac gacccgagca
     1741 ggttgaccac ggcgaagatc agcgccgagc cgaccatgcg cccctccccg cccagcgccg
     1801 acagggcgta ggccgagatc atggccagct cgaagaacac gtagaagctg aacacgtcgc
     1861 cggtcaggaa cagcccggtc aagccgacgg acaggaacag catcagggcc gggaacagcc
     1921 ggggcgacat gcccgagacc accccgtgca ccagcgcggc caggaccacg gcgaccgaga
     1981 ccagggccat gaccaccccc agggcgtcgg cgcgcagccg gatgccgaca tcggccggcc
     2041 agcccccggc gaccacctcg accggcccgt cggccgcgac ccgcagggcc agggcggcca
     2101 ggaacacgac cgcgacggcc agcaccgcca ccgcccccca gccggtggcc cgccggcgcc
     2161 cgtcggccag gacggccacg gccgcggccg gccacaacag ggccagcggc gccac
//
LOCUS       DASSWR010000666         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1297338, whole genome shotgun sequence.
ACCESSION   DASSWR010000666 DASSWR010000000
VERSION     DASSWR010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297338"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFO47_11200"
     CDS             complement(<1..378)
                     /locus_tag="VFO47_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789301.1"
                     /translation="MTAAAPFPAFELARKVADAVLYEGYLLYPYRASAAKNQARWQFG
                     VLLPRRWSEHGPEEPWSSQTECLLEPEESTTVRVLVRFLHVQAKTVEVVDLEAGTFDE
                     VPALPVDGAELVPWDEAAEQEVTV"
     gene            complement(375..1016)
                     /locus_tag="VFO47_11205"
     CDS             complement(375..1016)
                     /locus_tag="VFO47_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HET8789302.1"
                     /translation="MTDLVFDCVDAQPDRYAAVPTLQLKLRISETTGEQVHAIALRCQ
                     IRIEPQRRRYSPEEADGLLELFGEPPRWGDTLKPMQFATVSLMVPSFTGSIECDLPVP
                     CTYDFEVAASKYLHALGDGEVPLLLLFSGTVFTKGLSGFSVGQVPWHKETTYRLPVAV
                     WREIMDRFFPGAGWIRVRRDTLDSLQRFKAVRALPTWDDALEALFKEAGEARP"
     gene            complement(1013..1681)
                     /locus_tag="VFO47_11210"
     CDS             complement(1013..1681)
                     /locus_tag="VFO47_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HET8789303.1"
                     /translation="MSSGLRRFVGPQPAGPPVPGAAREKPRAAPGEACEMCAEPIGDG
                     HSHVVNLDSRQILCTCRACYLLFTHQGAAGGRYRAVPDRYLHDPGFRLDDASWDALQI
                     PVRVAFFFLNSDLGRVAGFYPSPAGATESLLPLEAWADLVAANPVMTDLVPDVEALLV
                     QRTGQAFECFLVPIDACYELVGLVRMHWKGFDGGQEAWEAIDGFFDAVRERSRPVGKE
                     TGEP"
     gene            complement(1678..2250)
                     /locus_tag="VFO47_11215"
     CDS             complement(1678..2250)
                     /locus_tag="VFO47_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET8789304.1"
                     /translation="MDETRDLSTVGARIEELIGRIRAAGDPATAETAEEVVRLVVELY
                     GAALERTVELAGPEVLERLVEDELVASLLVLHGLHPKDTETRVVEALDQVRPYLGSHA
                     GGVELLGVGPEGVVRLRLEGSCDGCPSSTMTVKLAIERAIEEAAPEVTAVEVENLTRE
                     REPQLLQIRTMRPEWQEPCPAVETGAGAGV"
     gene            complement(2261..4036)
                     /locus_tag="VFO47_11220"
     CDS             complement(2261..4036)
                     /locus_tag="VFO47_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET8789305.1"
                     /translation="MTTTERAGSGDGLVEMAWDPITRIVGSLGIYTKIDFAQKKVAEC
                     FSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHATCSVYNQNMAYGVRPPHLGE
                     WIINLGESAEYMFDHNIFQENLVGVDYCERMVKETNPGVLERANSTEAPHAADHGYRT
                     IGDIMRSLNPLEGEFYREALQVSRSTREMFCLMEGRHVHPSTLYPGGVGTVATVQLFT
                     DYLTRLMRYVEFMKRVVPMHDDLFDFFYEALPGYEQVGQRRVLLGCWGSLNDPEVCDF
                     RYETMTDWGRKMFVTPGVIVDGKLVTNDLTQINLGIRILLGSSYYEDWEDQPMFVTHD
                     PVGNPVDRRHPWNQHTIPKPQKRDFDNNYSWVMSPRWFDGTDHLALDTGGGPIARLWS
                     TALSGLVDIGYIKATGNSVVINLPRTATKPEKSFEWKIPQWSNAIERNRARTYFQAYA
                     AAAALHFTEKALAEIRAGHTKTWETFKVPDEAVSVGFTEAVRGVLSHHMVIRNGKIAN
                     YHPYPPTPWNGSVRDVYGTPGPYEDAVQNTPIFEENPPERFKGIDIMRAVRSFDPCLP
                     CGVHMYLGNGNVVKKLHSPTMLNQG"
     gene            complement(4049..>4419)
                     /locus_tag="VFO47_11225"
     CDS             complement(4049..>4419)
                     /locus_tag="VFO47_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HET8789306.1"
                     /translation="DRAGYYEQGDFATEYGSPKCIVKLGCWGPVVKCNVPKRGWLNGI
                     GGCPNVGGICIGCTMPGFPDKFMPFMDEPPGSKVSTTATTAYGAVIRTLRSVTARTVD
                     KEPKWRKTGRELTTGYRPSW"
BASE COUNT          622 a         1503 c         1590 g          704 t
ORIGIN      
        1 gaccgtcacc tcctgctcgg ccgcctcgtc ccaggggacc agctcggcgc cgtcgaccgg
       61 cagggccggg acctcgtcga aggtgccggc ctcgaggtcg accacctcga cggtcttggc
      121 ctgcacgtgg aggaagcgga ccaggacccg gacggtggtc gactcctccg gctcgagcag
      181 gcactcggtc tggctcgacc atggctcctc ggggccgtgc tcgctccagc ggcggggcag
      241 caggacgccg aactgccagc gggcctggtt cttggccgcc gaggcccggt aggggtacag
      301 caggtagccc tcgtacagga cggcgtcggc caccttgcgg gccagctcga aggcaggaaa
      361 cggggccgcg gccgtcacgg cctggcctcc ccggcctcct tgaacagcgc ctcaagggcg
      421 tcgtcccagg tcggcagggc ccggacggcc ttgaagcgct gcagcgagtc gagggtgtcg
      481 cgccgcaccc ggatccagcc cgccccgggg aagaagcggt ccatgatctc gcgccacacc
      541 gccaccggca gccggtaggt ggtctccttg tgccagggca cctgcccgac cgagaagccg
      601 ctcagcccct tggtgaacac cgtcccgctg aacagcagca gcagcgggac ctcgccgtcg
      661 ccgagggcgt gcaggtactt ggaggcggcc acctcgaagt cgtaggtgca gggcaccggc
      721 aggtcgcact cgatgctgcc ggtgaagctc ggcaccatca gcgacacggt ggcgaactgc
      781 atcggcttga gggtgtcgcc ccagcggggc ggctcgccga acagctccag cagcccgtcg
      841 gcctcctccg gcgagtagcg gcggcgctgg ggctcgatgc ggatctggca gcgcagggcg
      901 atcgcgtgga cctgctcgcc ggtcgtctcc gagatccgca gcttgagctg gagggtgggg
      961 acggccgcgt agcggtcggg ctgggcgtcg acgcagtcga acacgaggtc ggtcacggtt
     1021 ccccggtctc ctttccgacc gggcggctcc gctcccggac cgcgtcgaag aagccgtcga
     1081 tcgcctccca cgcctcctgg ccgccgtcaa agcccttcca gtgcatccgg accaggccga
     1141 ccagctcgta gcaggcgtcg atcgggacca ggaagcactc gaaggcctgg ccggtccgct
     1201 ggaccagcag cgcctcgacg tcggggacga ggtcggtcat gaccgggttg gcggcgacca
     1261 ggtcggccca ggcctccagg gggagcagcg actcggtggc cccggccggg ctcgggtaga
     1321 agccggccac ccgccccagg tcggagttga ggaagaagaa ggcgacccgc accgggatct
     1381 ggagggcgtc ccagctcgcg tcgtcgagcc ggaaccctgg gtcgtgcagg tagcggtcgg
     1441 gcaccgcccg gtagcgcccg ccggccgccc cctggtgggt gaacagcagg tagcaggccc
     1501 ggcaggtgca caggatctgg cggctgtcca ggttgaccac gtgcgagtgg ccgtcgccga
     1561 tcggctcggc gcacatctcg cacgcctccc ccggcgccgc ccgcggtttc tcccgggccg
     1621 cccccggcac cggcgggccg gccggctggg gccccacgaa ccgccgcagc cccgagctca
     1681 tacgccggca cccgcccccg tctcgacggc cgggcacggc tcctgccact cggggcgcat
     1741 cgtccggatc tggagcagct gcggctcccg ctcccgggtc aggttctcga cctcgacggc
     1801 ggtgacctcg ggggcggcct cctcgatggc ccgctcgatg gccagcttga ccgtcatggt
     1861 ggaggacggg cagccgtcgc agctgccctc caggcgcagg cggaccaccc cttcggggcc
     1921 gaccccgagc agctcgaccc cgccggcgtg ggagccgagg taggggcgga cctggtcgag
     1981 cgcctcgacc acccgggtct cggtgtcctt gggatggagg ccgtggagga cgagcaggct
     2041 ggcgaccagc tcatcctcca cgagccgttc gaggacttcg gggccggcca gctcgaccgt
     2101 ccgctccagc gccgccccgt acagctcgac gaccaggcgc acgacctcct cggcggtctc
     2161 cgcggtagcg gggtcgccgg cggcgcggat ccggccgatc agctcctcga tccgcgcccc
     2221 gaccgtgctg aggtcgcgcg tctcgtccat ggcggcggtt ctatccctgg ttgagcatgg
     2281 tcggcgagtg cagcttcttc accacgttgc cgttgcccag gtacatgtgg accccgcagg
     2341 gcaggcacgg atcaaagctg cggaccgccc gcatgatgtc gatgcccttg aacctctcgg
     2401 gtgggttctc ctcgaagatg ggggtgttct ggacggcgtc ctcgtacggc cccggggtgc
     2461 cgtagacgtc gcgcacgctg ccgttccacg gcgtcggcgg gtaggggtgg tagttggcga
     2521 tcttgccgtt gcggatgacc atgtggtgcg agagcacgcc ccgcacggcc tcggtgaacc
     2581 cgacgctcac ggcctcgtcg ggcaccttga aggtctccca ggtcttggtg tggccggccc
     2641 ggatctcggc cagcgccttc tcggtgaagt gcagggccgc ggcggccgcg tacgcctgga
     2701 agtaggtgcg ggcccggttg cgctcgatcg cgttggacca ctgcgggatc ttccactcga
     2761 aggacttctc gggcttggtg gccgtccggg gcaggttgat gaccacgctg ttgccggtgg
     2821 ccttgatgta gccgatgtcg accaggccgg acagggccgt cgaccacagc cgggcgatcg
     2881 gcccgccgcc ggtgtccagg gccaggtggt cggtgccgtc gaaccagcgc ggcgacatca
     2941 cccacgagta gttgttgtcg aagtcgcgtt tctggggctt ggggatggtg tgctggttcc
     3001 aggggtggcg gcggtcgacc gggttcccca ccgggtcgtg ggtcacgaac atcggctggt
     3061 cctcccagtc ctcgtagtag gaggagccca gcaggatgcg gatgcccagg ttgatctggg
     3121 tcaggtcgtt ggtgacgagc ttgccgtcga cgatcacgcc cggggtcacg aacatcttgc
     3181 ggccccagtc ggtcatggtc tcgtagcgga agtcgcacac ctccgggtcg ttgaggctgc
     3241 cccagcagcc gagcaggacg cggcgctggc cgacctgctc gtagccgggc agggcctcgt
     3301 agaagaagtc gaacaggtcg tcgtgcatgg gcacgacccg cttcatgaac tcgacgtagc
     3361 gcatcaggcg ggtcaggtag tcggtgaaca gctgcacggt ggcgaccgtg ccgacgccgc
     3421 ccgggtacag ggtcgagggg tggacgtggc ggccctccat caggcagaac atctcccggg
     3481 tggagcggct aacctggagc gcctcgcggt agaactcgcc ctcgagcggg ttcagcgagc
     3541 gcatgatgtc gccgatcgtc cggtagccgt ggtcggcggc gtggggcgcc tcggtcgagt
     3601 tggcccgctc cagcaccccc gggttggttt ccttgaccat ccgctcgcag tagtcgaccc
     3661 cgaccaggtt ctcctggaag atgttgtggt cgaacatgta ctcggcagac tcgcccaggt
     3721 tgatgatcca ctcgcccagg tgcggcgggc gcaccccgta ggccatgttc tggttgtaga
     3781 ccgagcaggt ggcgtggttg tccccgcaga tgccgcagat gcggctggtg atgaagtggg
     3841 cgtcgcgcgg gtccttgccc ttcatgaaga tcgagtagcc gcggaagatc gacgaggtcg
     3901 agaagcactc ggcgaccttc ttctgggcga agtcgatctt ggtgtagatg ccgaggctgc
     3961 cgacgatccg ggtgatcggg tcccaggcca tctcgacgag gccgtcaccg ctcccggcgc
     4021 gttcggtcgt ggtcatcgtc gccctcccct accagctcgg acggtagccg gtggtcagct
     4081 cacggccggt cttgcgccac ttcggctcct tgtcgaccgt cctggcggtg acgctgcgca
     4141 aggtgcggat caccgccccg taggcggtgg tcgccgtggt ggacaccttg ctgccgggcg
     4201 gctcgtccat gaacggcatg aacttgtccg ggaagcccgg catggtgcag ccgatgcaga
     4261 tgccgccgac gttggggcag ccgccgatgc cgttgagcca gccccgcttg ggcacgttgc
     4321 acttgaccac cggaccccag cagccaagct tgacgatgca cttgggggag ccgtactccg
     4381 tggcgaagtc gccctgctcg tagtagccgg cccggtcac
//
LOCUS       DASSWR010000667         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_476617,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000667 DASSWR010000000
VERSION     DASSWR010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476617"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFO47_11230"
     CDS             <1..468
                     /locus_tag="VFO47_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789307.1"
                     /translation="HLIGVHARGTDATSRQELRPFRQGSLVLSRYVAEIERLLDTQPR
                     AKILVASDEQATLDHLASAFGVRVIAYDTVRHRDGEAAGQGPTGWIMPAYIARDRDVA
                     ARNGEDAVIEYLLLSRCHQLVHNGSSLARTVLLNAPELPHTNTHRRSVVRDSR"
     gene            455..1330
                     /locus_tag="VFO47_11235"
     CDS             455..1330
                     /locus_tag="VFO47_11235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8789308.1"
                     /translation="MTAVDRRTIERVKAEKRRRRSSSHRYQDRPRLAFVVHSFNRIAN
                     IDQLVGGLRSMGDHELIVCEDGSLDGSREKWLSYLNRPNDFLIHSNDLHEIRVLDRAI
                     RFARADVVCLVQDDDRIPRQTDWLDAALGAFDDHPRLAILGGFQAYGSFDPDPAKARR
                     IWGGDQFRFVHHVNIGPYFIRRRHYELLGGWEYSFSKVGEPGICADNELCLRAWTNGY
                     QVGYRFVPFKGPVGHYPQDGGTVLFSKETRVRNAVRNSDTIFELYGPHARRIDELVAE
                     ANAGLRSDGKLAPRP"
     gene            complement(1327..2181)
                     /locus_tag="VFO47_11240"
     CDS             complement(1327..2181)
                     /locus_tag="VFO47_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET8789309.1"
                     /translation="MPCPVRAFAGVDLASLDPFVHMDQMGEIDYAPGEPKGTPWHPHR
                     GFETVTYMIDGVFRHQDSNGGGGLITDGDTQWMTTGGGILHIEAPPEEVVMRGGLFHG
                     FQLWVNLPARLKMTPPRYQDIRGGAVTLLSSPDGGALLRVIAGELGGHQGPGITYTPI
                     SLLHATLAPGARLELPWRADFNALVYVLAGSGSVGSKRHPVRTGQLAVYGPGDVVTLE
                     AATSQESRHQQLDVLVLGGQPIREPVAAYGPFVMNTRAELIQAFEDYQAGRLGSIPAE
                     PRPGHEVL"
     gene            2183..>2561
                     /locus_tag="VFO47_11245"
     CDS             2183..>2561
                     /locus_tag="VFO47_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014053639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET8789310.1"
                     /translation="MVASDGGLLLSTAPQGYEGEGFPVRRAFAGIDLQHLDPFIMMDQ
                     MGEVEYAPGEPKGTPWHPHRGFETVTYIIDGIFDHQDSNGGGGTITNGDTQWMTAGSG
                     ILHIESPPEWLVESGGLFHGIQLW"
BASE COUNT          396 a          924 c          877 g          364 t
ORIGIN      
        1 cacctcatcg gcgtgcatgc ccgggggacc gacgcgacct ccaggcagga gctccgcccg
       61 ttccgtcagg gttcgctggt gctctcgcgg tacgtcgctg agatcgagcg cctgctggac
      121 acccagccac gggccaagat cctggtggca tcggacgagc aggcgaccct ggaccacctg
      181 gcgtcggcgt tcggcgtccg cgtgatcgcc tacgacaccg tccggcaccg ggacggtgag
      241 gcggccggac agggcccgac cggctggatc atgccggcct acatcgcccg ggaccgcgac
      301 gtggccgccc ggaacgggga ggacgcggtc atcgagtacc tgctcctgag ccggtgccac
      361 cagctcgtcc acaacgggtc gagcctggcc aggaccgtcc tgctgaacgc ccctgagctg
      421 ccgcacacca acacccaccg aaggagcgtc gtccgtgaca gccgttgacc ggaggacgat
      481 cgagcgcgtc aaggcggaga agcggcggcg gcggtcctcc tcccaccgct accaggaccg
      541 gcccaggctg gcgttcgtcg tccattcctt caaccgcatc gccaacatcg accagctggt
      601 cggcggcctc aggagcatgg gcgaccatga gctgatcgtc tgtgaggacg gctcgctcga
      661 cggctcccgc gagaagtggc tgtcctacct caaccggccc aacgacttcc tgatccactc
      721 gaacgacctg cacgagatcc gggtcctcga ccgggcgatc cggttcgccc gcgcggacgt
      781 cgtctgcctg gtccaggacg acgaccgcat ccctcgccag accgactggc tggacgccgc
      841 gctcggggcc ttcgacgacc acccccggct ggccatcctc ggcggcttcc aggcctacgg
      901 gagcttcgac ccggaccccg ccaaggcgag gcgcatctgg ggaggcgacc agttccgctt
      961 cgtccaccac gtgaacatcg gcccctactt catccgcagg cggcactacg agctgctggg
     1021 aggctgggag tactccttct ccaaggtcgg cgaaccaggc atctgcgccg acaacgagct
     1081 ctgcctccga gcctggacga acggctacca ggtcggctac cgcttcgtcc ccttcaaggg
     1141 cccggtgggg cactacccgc aggacggagg caccgtgctg ttctcgaagg agacgagagt
     1201 caggaacgcg gtccggaact ccgacacgat cttcgagctg tacggaccgc acgcccggcg
     1261 gatcgacgag ctcgtcgccg aggccaacgc gggactccgg tccgacggca agctggcccc
     1321 caggccctag agcacctcgt ggccggggcg gggctcggcc gggatggagc cgaggcggcc
     1381 ggcctggtag tcctcgaacg cctggatgag ctcggcgcgg gtgttcatca cgaacggccc
     1441 gtaggcggcc accggctcgc ggatgggctg cccgcccagg accaggacgt cgagctgctg
     1501 gtggcggctc tcctggctcg tggccgcctc cagcgtcacc acgtcgccgg gcccgtagac
     1561 ggccagctgg ccagtgcgca ccgggtggcg cttcgagccg accgaccctg acccggcgag
     1621 cacgtacacc agggcgttga agtcggcccg ccacggcagt tccagcctgg ctccgggggc
     1681 cagggtggcg tgcagcagcg agatcggcgt gtaggtgatg cccgggccct ggtggccgcc
     1741 gagctcgccg gcgatcaccc gcagcagggc gccgccgtcg ggcgagctca gcagggtgac
     1801 cgcgccgccg cggatgtcct ggtagcgggg cggggtcatc ttcagccggg ccggcaggtt
     1861 gacccagagc tggaagccgt ggaacagccc gccccgcatc accacctcct cgggcggcgc
     1921 ctcgatgtgg aggatcccgc cgcccgtggt catccactgc gtgtcgccgt cggtgatcag
     1981 cccgccgccg ccgttggagt cctggtgccg gaagacgccg tcgatcatgt aggtgacggt
     2041 ctcgaacccg cggtgcgggt gccagggggt gcccttgggc tcgcccgggg cgtagtcgat
     2101 ctcgcccatc tggtccatgt gcacgaacgg gtcgagactg gccaggtcca ccccggcgaa
     2161 cgcccggacc gggcacggca ccgtggtcgc cagcgacggc gggctgctgc tcagcacggc
     2221 cccgcagggc tacgagggcg agggcttccc ggtccgccgc gctttcgccg gcatcgacct
     2281 gcagcacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2341 tgagcccaag ggcaccccct ggcatcccca ccggggcttc gagaccgtca cctacatcat
     2401 cgacgggatc ttcgaccacc aggactccaa cggcggtggc ggcaccatca ccaacggcga
     2461 cacccagtgg atgaccgccg gatcggggat cctccacatc gagtcaccgc ccgagtggct
     2521 ggtcgagtca ggcggtctgt tccacggcat ccagctctgg g
//
LOCUS       DASSWR010000668         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_449238,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000668 DASSWR010000000
VERSION     DASSWR010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449238"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VFO47_11250"
     CDS             <1..137
                     /locus_tag="VFO47_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HET8789311.1"
                     /translation="DTARTAAAYGANYQRLQRVKATYDPDNLFRVNRNIPPAGIGSAG
                     "
     gene            513..1412
                     /locus_tag="VFO47_11255"
     CDS             513..1412
                     /locus_tag="VFO47_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HET8789312.1"
                     /translation="MAVDRQMVARTVSELRAALPEADDLLVGLLRVAEATRTVLEVDG
                     AGLTLVHEEGPPRWVAATDAAMELLEQVQQDFGEGPSLEAYAQDQPIRVEDLRTAPAR
                     TRIDAVVGQLHVCAVLSVPIRLADQPVGTLDLYSTQPRAWTAHELEAAREFAAVAGEL
                     VHTSVELASHKLEVAQLRQALTSRVWIEQAKGVLAATHGTDPEVAFQQLRTRARASSR
                     KLAELAREVVQDAQRERLAALALDDVRVREAEVRAQAAEQALQDAQADLAELRAALDR
                     AQVAAEERDRFADERDRTAGHPD"
     gene            complement(1871..>2387)
                     /locus_tag="VFO47_11260"
     CDS             complement(1871..>2387)
                     /locus_tag="VFO47_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789313.1"
                     /translation="PVSATGPLLLIRGLAGIVGVAAGGALVDRSPWAAMLVPVTAQAT
                     ALLGLYALGGTPVAAVGLVALSGLSFAALTTALASRVLQVAPGSADLAAAGASTAVNV
                     GITAGALIGSILLPGLGVRSTALVGGLLSLAALGVALCEPLVAANGRMRVTICPRFLA
                     GGAGGRAAASG"
BASE COUNT          343 a          844 c          904 g          296 t
ORIGIN      
        1 acgacaccgc ccggaccgcc gccgcctacg gggccaacta tcagcgcctc caacgcgtga
       61 aggccaccta cgaccccgac aacctgttcc gcgtcaaccg caacatcccc cctgcgggca
      121 ttgggagcgc tggatgagca gcgagggcga gggcctggcc gccagctcgc cgcgatccgc
      181 ggggtgacgc tggtcctggc cgcgaggctg gtgcacgtcc acggggctgg tggccggcct
      241 gggtcgacct gaagggacat gccggcgggg tctgggccga cgatatcccc tcaggctggt
      301 tcggtcgcgc cggacaggca gttgggcccg accacgacca ggcgccggat agccctccaa
      361 cacccgagac aaccagcggg tcatggcagg agccggccgg gttgtctcgg gtggaaacca
      421 gatcccctgg tcgggggccg cctggcgttg cccaagcggc ctggcgatgg aagcattctc
      481 tggttgtcgc cgtcgccatg gggaacaagg ctgtggcagt tgaccgtcag atggtcgccc
      541 ggaccgtcag cgagctgcgg gcggcgctgc ctgaagccga tgacctgctg gtcgggctgc
      601 tgcgggtcgc cgaggccacc cggaccgtcc tggaggtcga cggggccggg ctcaccctcg
      661 tccatgagga gggcccgcct cggtgggtgg ccgccaccga cgccgccatg gaactgctcg
      721 agcaggtcca gcaggacttt ggcgaaggcc cttccctgga ggcctatgcc caggaccaac
      781 ccatcagggt ggaggatctg cgaaccgcgc cggccaggac ccggatcgac gcggtggtcg
      841 gccagctgca cgtctgcgcg gtgctgagcg tccccatccg cctggctgac cagccggtgg
      901 ggacgctgga tctctacagc acccagccgc gggcctggac cgcccacgag ctggaggcgg
      961 cacgtgagtt cgcggccgtg gccggtgagc tggtccacac cagcgtggag ctggccagcc
     1021 acaagctgga ggtcgcccag ctacgccagg cgttgaccag ccgggtctgg atcgagcagg
     1081 ccaaaggcgt gctcgccgcc acccacggca ccgaccccga ggtggcgttc cagcagttgc
     1141 ggactcgggc tcgggcgagc tcgcgcaagc tggccgagct ggcccgggag gtcgtccagg
     1201 acgcccagcg tgaacggctg gccgccctgg ccctggacga tgtccgggtg cgggaggccg
     1261 aggtccgcgc ccaggccgcc gagcaggccc tccaggacgc ccaggccgac ctggccgagc
     1321 tccgggcggc gcttgaccgg gctcaggtcg ctgccgagga gcgggaccgc ttcgccgacg
     1381 aacgggaccg gacggccggt cacccggact gacatccacg tgctctggag ccgggcgccc
     1441 ggtcggagcc gctcgaaggc ggcagtcggc cccggggtgg atgttggcga tggctgccgg
     1501 cagccgttgg ctgaaggcca ccgcacacgg gccctggggg agaggtcctg accgtcccca
     1561 acattctggg cggtctggtc cgtcgccggc ccatcggagc tggcccttgg cgtcgaccgg
     1621 catgagcccg gcggcgtcgg ccgagaacag ctgcttggcg gcatcggtga tctgctgcgg
     1681 ggtcggcgcc gggccagctt cagggtcagg gatacccagg gcgccgatgc tcggacggtc
     1741 tgccgtccgg cggggcagat tggcagctga cccggctggc tatccggccc gggcagcctc
     1801 gggctggcac cgcttggtgc ggacgcgctc cccgcgggca ggttggccca accctcgctg
     1861 gcctgtaccg tcagcccgag gctgctgctc gcccgccagc accgcctgcc aggaaacgag
     1921 ggcagatggt tacccgcatg cggccgttgg cggcgaccag cggctcgcag agggcgacgc
     1981 cgagggcggc gaggctcagc aggccgccga cgagggcggt gctgcggaca ccgaggccgg
     2041 gcagcaggat gctgccgatc aacgcgccgg cggtgatgcc gacgttgacc gcggtggacg
     2101 cgccggcggc cgccaggtcg gcgctgcccg gcgcgacctg aagcacccgg ctggccaggg
     2161 cggtggtcag ggcggcgaac gacagccctg acagcgcgac cagcccgacg gccgctaccg
     2221 gcgtcccgcc cagagcgtag aggccgagca gcgccgtggc ctgggccgtg accgggacga
     2281 gcatcgccgc ccatgggctg cggtcgacca gggcgccgcc ggcggcgacc ccgacgatgc
     2341 ccgccaggcc acgaatcagc agcagcggcc cggtcgccga caccggg
//
LOCUS       DASSWR010000669         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_308002,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000669 DASSWR010000000
VERSION     DASSWR010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308002"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1247
                     /locus_tag="VFO47_11265"
     CDS             51..1247
                     /locus_tag="VFO47_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8789314.1"
                     /translation="MSYRVREVAERAGVPEAFVRRLIAAGALPGEEQGLGPREVRRAR
                     LLHSWEAAGMSIETIVALVERGALSLAFLDAPVMETPERLDRSYAQLAADRQVPLALV
                     QGIHQATGFAPPEAGDRAGEDDVTMLELAELFRGAGVDDDATLRLLAVYADSVRRIAK
                     AEADYYEANVERRLRATGLDERQLIEFGTRLGGRVVGSLERAIFQLYRRHREHVWTEH
                     AINHVEEALEGSGLRERVPRPPAICFVDLTGFTRLTEERGDEVAARVAGALAALVNDI
                     SRGRGGRPVRWLGDGGMFHFREPGAAVTAGLDMVERAPAAGLPPAHVGIHTGAVVAQD
                     GDVYGMTVNLAARISSYAEAGQVVVSAETAARAAGAGLRFEPLGPVELKNVARPLPLY
                     RALRGP"
     gene            complement(1250..2206)
                     /locus_tag="VFO47_11270"
     CDS             complement(1250..2206)
                     /locus_tag="VFO47_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HET8789315.1"
                     /translation="MTPLVDWRAMDEGDVFQELRPLLFSIAYRMVGSVSEAEDLVQEA
                     FLRLRRAVAGGAEVESPKAYLTKVTTRLAIDHLRSARVRRERYVGAWLPEPLVTGPGA
                     WADPGVADPAGHAETAESVSLAFLHLLERLSPVQRAVFVLHDVLGYGYAEVAGLVGRS
                     EETCRQIAARARRHVEGGRPRFEASRRQRDELARRFFAAVEQGDTEGLVGLLAGDAVL
                     YGDGGGKAPALTAPLLGAERVARILLGLARRGAAVGVGMRPAEINGHPGVLTSGPDGR
                     LLGVLAVDIAGGRVRTVYSIVNPDKLRHLGPVGDLTALLGNG"
     gene            2265..>2431
                     /locus_tag="VFO47_11275"
     CDS             2265..>2431
                     /locus_tag="VFO47_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET8789316.1"
                     /translation="MRIFVAGATGALGRRLVPLLVSGGHQVTGMTRSPGKAAWLRAAG
                     AEPVVADALDR"
BASE COUNT          309 a          877 c          962 g          283 t
ORIGIN      
        1 gctcccgcca ctgatgggga acaatcccgg gcgggagaag ggaggccggg atgtcgtacc
       61 gcgtcaggga ggtcgccgag cgggccggcg tcccggaggc cttcgtccgg cggctgatcg
      121 cggccggcgc cctccccgga gaggagcagg gcctgggccc ccgggaggtc cgccgggccc
      181 ggctgctgca ctcgtgggag gcggcgggga tgtcgatcga gacgatcgtc gccctggtcg
      241 agcggggcgc cctgtcgctc gcgttccttg acgcgcccgt gatggagacg ccggagcggc
      301 tggaccggtc gtatgcgcag ctggccgccg accgccaggt gcccctggcc ctcgtgcagg
      361 ggatccacca ggcgaccggc ttcgcgcccc cggaggccgg cgaccgggcc ggcgaggacg
      421 acgtgaccat gctggagctg gccgagctgt tccggggcgc cggggtcgac gacgacgcca
      481 ccctccggct gctggccgtc tacgccgaca gtgtccgccg gatcgccaag gccgaggccg
      541 actactacga ggccaacgtc gagcggcggc tccgggccac cggcctggac gaacggcagc
      601 tgatcgagtt cggcacgcgg ctcggcggcc gcgtcgtcgg gtcgcttgag cgggccatct
      661 tccagctcta ccggcgccac cgcgagcacg tctggaccga gcacgccatc aaccatgtcg
      721 aggaggccct cgagggatcg gggctgcgcg aacgggtccc gcggccaccc gcgatctgct
      781 tcgtcgacct gaccgggttc acgcggctga ccgaggagcg cggggacgag gtggccgccc
      841 gggtcgccgg ggccctggcc gcgctggtca acgacatctc gcgcggccgg ggaggccggc
      901 ccgtgcgctg gcttggggac ggcgggatgt tccacttccg ggagcccggg gcggccgtca
      961 ccgccgggct ggacatggtg gagcgggccc cggcggccgg gctgccgccg gcccacgtcg
     1021 gcatccacac cggcgcggtg gtcgcccagg acggcgacgt gtacgggatg acggtcaacc
     1081 tggccgccag gatctcctcg tacgccgagg ccggccaggt cgtggtcagc gcggagaccg
     1141 cggcccgcgc cgccggcgcc ggcctgcggt tcgagcccct ggggccggtc gagctgaaga
     1201 acgtcgccag gcccctgccg ctctaccggg cgctccgcgg gccctgagct cagccgttgc
     1261 cgagcagggc ggtcaggtcg ccgaccgggc cgagatggcg cagcttgtcg gggttgacga
     1321 tcgagtagac ggtccggacc cggccgccgg cgatgtcgac ggcgaggacg cccagcaggc
     1381 ggccgtcggg cccggacgtg agcacgcccg ggtggccgtt gatctcggcc gggcgcatgc
     1441 cgacgccgac ggcggcgcct cggcgggcca ggccgaggag gatgcgcgcg acccgctcgg
     1501 cgcccaggag gggtgcggtc agggcggggg ccttgccgcc gccgtcgccg tagaggacgg
     1561 cgtcgccggc cagcaggccg accaggccct cggtgtcgcc ctgctcgacg gcggcgaaga
     1621 agcggcgggc cagctcgtcg cgctggcgcc gggaggcctc gaaccgggga cggccgccct
     1681 cgacgtggcg gcgggcccgg gcggcgatct ggcggcaggt ctcctcggac cggccgacca
     1741 gcccggcgac ctcggcgtag ccgtagccga gcacgtcgtg gagcacgaac acggcccgct
     1801 ggaccggtga caggcgctcc agcaggtgga ggaaggccag cgacacgctc tcggcggtct
     1861 cggcgtggcc ggccgggtcg gccacgcccg ggtccgccca ggcccctggg ccggtcacca
     1921 gcggttccgg cagccaggcg ccgacgtagc gctcgcgccg gacccgggcc gagcgcaggt
     1981 ggtcgatggc caggcgggtc gtgaccttcg tcaggtacgc cttgggcgac tccacctcgg
     2041 cgccgccggc cacggcccgg cgcagccgca ggaacgcctc ctggaccagg tcctcggcct
     2101 cgctgacgct gcccaccatc cggtaggcga tcgagaacag cagcggccgc agctcctgga
     2161 agacatcacc ctcgtccatc gccctccagt caaccagagg tgtcacggcc gggccggcca
     2221 cctcgtctac gaggggaaac gacgacacaa ccaaggagct gaccatgagg atcttcgtcg
     2281 ccggggcgac cggggcgctg ggccgccggc tggtgccgct gctggtgtcg ggcgggcatc
     2341 aggtgacggg catgacccgg tcgccgggca aggccgcctg gctgcgggcg gccggggccg
     2401 agccggtggt ggccgacgcc ctcgaccggg a
//
LOCUS       DASSWR010000670         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_308004,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000670 DASSWR010000000
VERSION     DASSWR010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308004"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1430)
                     /gene="der"
                     /locus_tag="VFO47_11280"
     CDS             complement(120..1430)
                     /gene="der"
                     /locus_tag="VFO47_11280"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET8789317.1"
                     /translation="MVGRPNVGKSTLVNRILGRRAAITQEVPGVTRDRVAYQADWSGR
                     RFELVDTGGWEPKATGLQASVVAQAERAMADADLLLFVVDATVGVTEEDAAVAARLRR
                     GKVPVLLVANKVDSAAAEPQVADLWSLGLGEPLAVSSLHGRGAGDLLDAVIKRLGTEA
                     AVGDEADAPALAIVGRPNVGKSSLLNRLAGQDLAIVDPTPGTTRDTVDSIVERAGRSY
                     RLVDTAGMRRSFQQAEGADYFAFVRSLEAVDRADVALLVLEAPLGVAEQDQKVAVRAV
                     EAGCGLVLLANKWDLMDDEARRQFGVDLERKLYFVDWAPMLRVSARTGRGVEKIWGLA
                     DGVLAERSRRIPTAALNQWLGQATERTPPPPVRGRSVKVRYATQTRVDPPEVVLFTSG
                     QLTPSYRRYLERDLRRTFGFEGTPVRLVVRVRTRERPAGADRRG"
     gene            complement(1495..2160)
                     /locus_tag="VFO47_11285"
     CDS             complement(1495..2160)
                     /locus_tag="VFO47_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8789318.1"
                     /translation="MPQRGNPDRGYLIAGAVVKPLMRVWFRMRVEGREHVPAEGPVIL
                     ASNHRSNMDPVLLASAVKRPVAFMAKAELFVGPLGWIMRWIGQFPVRRGGIDREALRR
                     TDAILARGSTLGLFPEGTRGDGRFSAVHPGLAYIVVRQRCPVLPVAIFGTERVRRRFG
                     WLPFASPVRVVIGPAIDLPPSSGDRAGRRAASEVLRQRLQAFLATASGVPSLPATSSQ
                     VNE"
BASE COUNT          294 a          856 c          798 g          286 t
ORIGIN      
        1 gcgaccccca gtcccccaga ctggtgcgct aaccaagctg cgccacgtcc cgagcgcggg
       61 cccgaaggcc cgaaacggcc gtcaggatat cacggccatt ggcgcctccc gccggccggc
      121 tagccccggc ggtcggcgcc cgcgggccgc tcgcgggtcc ggacccggac caccagccgg
      181 accggcgtgc cctcgaaccc gaaggtccgc cgcaggtccc gctccaggta gcggcggtag
      241 ctgggggtga gctggccgct ggtgaacagg accacctcgg gcgggtcgac cctggtctgg
      301 gtggcgtagc ggaccttgac cgaacggccg cgcaccggcg gcggcggcgt gcgctcggtc
      361 gcctgcccca gccactggtt gagggccgcg gtggggatgc gccgggaccg ctcggccagc
      421 accccgtcgg ccaggcccca gatcttctcg accccccggc cggtgcgggc cgagacgcgc
      481 agcatcggcg cccagtccac gaagtacagc ttgcgctcca ggtcgacgcc gaactggcgc
      541 cgcgcctcgt cgtccatgag gtcccacttg ttggccagca ggaccagccc gcagccggcc
      601 tcgaccgccc ggacggccac cttctggtcc tgctcggcca cgcccagggg cgcctcgagc
      661 acgagcaggg ccacgtcggc ccggtcgacc gcctccaggc tgcgcacgaa ggcgaagtag
      721 tcggccccct cagcctgctg gaatgagcgc cgcatgccgg cggtgtcgac cagccggtag
      781 ctccgtccgg cccgctccac gatcgagtcg acggtgtcgc gggtcgtgcc cggggtcggg
      841 tcgacgatgg ccaggtcctg cccggccagc cggttgagca ggctcgactt gccgacgttg
      901 ggccggccga cgatggccag cgccggcgcg tcggcctcgt cgccgaccgc ggcctcggtg
      961 ccgagccgct tgatgaccgc gtccagcagg tccccggccc cgcggccgtg gaggctggac
     1021 acggccagcg gctcccccag gccgagcgac cacaggtcgg cgacctgcgg ctcggcggcg
     1081 gcgctgtcga ccttgttggc gaccagcagc accggcacct tgccgcggcg caggcgggcg
     1141 gcgacggcgg cgtcctcctc ggtgaccccg accgtcgcgt ccacgacgaa cagcagcagg
     1201 tcggcgtcgg ccatggcccg ctcggcctgg gcgacgacgc tggcctggag gccggtcgcc
     1261 ttcggctccc agccgccggt gtcgaccagc tcgaagcgcc ggccggacca gtcggcctgg
     1321 taggcgaccc ggtccctggt cacgccgggg acctcctggg tgatggccgc ccggcggccc
     1381 aggatccggt tgaccagggt cgacttgccg acgttgggcc ggccgaccac ggcgaccctc
     1441 ggcaggtggc ctgctagccg atcttctcgt tgctccattg acatgtcgct ttgtctactc
     1501 attgacttgg ctacttgtgg ccggcagtga cgggacaccg ctcgccgtgg ccaggaaggc
     1561 ctggaggcgc tggcgcagga cctcgctggc ggctcggcgg ccggcccggt cgccggacga
     1621 gggtggcagg tcgatggccg ggccgatcac gaccctgacc ggggaggcga acggcagcca
     1681 accgaaccgg cgccgcaccc gctcggtgcc gaagattgcc accggcagca ccgggcagcg
     1741 ctggcggacc acgatgtagg cgagcccggg atggacggcc gagaaccgcc cgtcccccct
     1801 ggtcccctcg gggaacaggc ccagcgtcga gccgcgggcc aggatcgcgt ccgtccgccg
     1861 cagggcctcc cggtcgatgc cgccccgccg gaccgggaac tgcccgatcc agcgcatgat
     1921 ccagccgagc ggcccgacga acagctcggc cttggccatg aaggccaccg gccgcttcac
     1981 cgccgaggcg agcagcaccg ggtccatgtt gctgcgatgg ttcgaggcca ggatcaccgg
     2041 cccctcggcc ggcacatgct ccctcccctc gacccgcatc cggaaccaga cccgcatcaa
     2101 cggcttcacc accgccccag cgatcaggta cccccggtcg gggttcccac gctgaggcac
     2161 cttcgggtcg ggctggtcgc cccgacgggg gcttccagac cgctggggtg ctgcgggttc
     2221 ggactcggcc ggtc
//
LOCUS       DASSWR010000671         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_195062,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000671 DASSWR010000000
VERSION     DASSWR010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195062"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFO47_11290"
     CDS             complement(<1..217)
                     /locus_tag="VFO47_11290"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HET8789319.1"
                     /translation="MPDPLDPFEPQDDSPPEPIDEEERAALLDDLADLAEFRSALEQR
                     GFLGVVISCPDCEEDHFFGWSLLRENLE"
     gene            417..1577
                     /locus_tag="VFO47_11295"
     CDS             417..1577
                     /locus_tag="VFO47_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET8789320.1"
                     /translation="MAEVEIGIGKSGRRAYGFDDIAIVPSRRTRDPDDIDISWDLDAY
                     HFELPLLASAMDGVVSPRIAVELGRLGGLGVLNLEGLWTRYEDPDPVYAEIAGLPDAD
                     ATKRMQEIYAEPIKPELIGRRVAEIKEAGVVAAASLTPQRVARYHQFALEAGLDILVI
                     QGTVVSAEHVSSTSEPLNLKTFIAHYDLPVIVGGCASYATALHLMRTGAAGILVGVGP
                     GAACTTRGVLGIGVPMATALADAAGARIRHLEETGRYVQVIADGGMRTGGDVAKAIAC
                     GADAVMIGSPLARAVEAPGRGHHWGMATFHPDLPRGTRVRTTSLGTLKEILLGPAPVN
                     DGSMNLFGGLRTSMATTGYQTVKEFQKAEVMVAPALQTEGKLLQRSQGVGMG"
     gene            complement(1585..1797)
                     /locus_tag="VFO47_11300"
     CDS             complement(1585..1797)
                     /locus_tag="VFO47_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789321.1"
                     /translation="MTKPEEPIPAPTRRRAPGADPAVRSFGQARTCAADGCETRLSRY
                     NPDTFCSVHRGWDRQVVTRRRKGDDE"
     gene            1959..>2522
                     /gene="guaA"
                     /locus_tag="VFO47_11305"
     CDS             1959..>2522
                     /gene="guaA"
                     /locus_tag="VFO47_11305"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET8789322.1"
                     /translation="MTTPVPIEASLAEVEAARPEPGALRARPAPGHDTVLVLDFGAQY
                     AQLIARRIRECHVFSEVVPCDVDPDTVRAHDAKALVLSGGPKSVYEPGAPALDPRLLE
                     LGIPVLGLCYGQQAMARALGGRVEATGVREYGRTTLRAQPEGSLLFGDLPEEQVVWMS
                     HGDTVTAAPPGARVVASTDASPVAGFED"
BASE COUNT          349 a          953 c          882 g          338 t
ORIGIN      
        1 gctccaggtt ctcgcgcagc aggctccagc cgaagaagtg gtcctcctcg cagtcggggc
       61 aggagatgac caccccgagg aagccgcgct gctccagcgc cgagcggaac tcggccaggt
      121 cggcgaggtc gtccaggagc gcggcgcgct cctcttcgtc gatgggttcg gggggcgagt
      181 cgtcttgcgg ctcgaacgga tcgagggggt cgggcacggg cactccgaag tcaggttgat
      241 gcgaagctct ccctggcatg gtagcgcgga tgccgatcgg gctctccttg acctccacag
      301 aaccccgctg ctagcgtcgg acatgcacct tccccgggcc cgccgcgcca gcggacgggt
      361 ccgttcgtct atctgcaccc aaacccacga ggtaccgctg ctgggaggaa ggcaacgtgg
      421 ctgaggtcga gatcggcatc ggcaagagcg gccgccgggc gtacgggttc gacgacatcg
      481 cgatcgtgcc cagccggcgg acgcgcgacc ccgacgacat cgacatctcc tgggacctgg
      541 acgcctacca cttcgagctg cccctgctgg cctcggccat ggacggcgtc gtgtcgcccc
      601 ggatcgcggt cgagctgggc cgcctcggcg ggctcggcgt gctcaacctg gagggtctct
      661 ggacccgcta cgaggacccc gacccggtct acgccgagat cgccggcctg cccgacgccg
      721 acgccaccaa gcggatgcag gagatctacg ccgagccgat caagccagag ctgatcggcc
      781 ggcgggtggc cgagatcaag gaggctgggg tggtggcggc cgcctccctc accccccagc
      841 gggtcgcccg ctaccaccag ttcgccctcg aggcggggct ggacatcctg gtcatccagg
      901 ggacggtggt ctcggccgag catgtctcgt ccaccagcga gccgctcaac ctcaagacct
      961 tcatcgccca ctacgacctg ccggtgatcg tgggcggctg cgcctcctac gccaccgccc
     1021 tgcacctgat gcgcaccggg gcggccggga tcctggtcgg ggtcgggccg ggggcggcct
     1081 gcaccacccg cggcgtgctc ggcatcggcg tgcccatggc caccgccctg gccgacgccg
     1141 ccggggcccg catccggcac ctggaggaga cgggccgcta cgtgcaggtg atcgccgacg
     1201 gcggcatgcg caccggcggc gacgtggcca aggcgatcgc ctgcggggcc gacgccgtga
     1261 tgatcggctc ccccctggcc agggcggtcg aggcccccgg gcgcggccac cactggggca
     1321 tggccacctt ccaccccgac ctgccacggg ggacccgggt gcgcaccacc tccctcggca
     1381 ccctcaagga gatcctgctc ggcccggcgc cggtcaacga cggcagcatg aacctgttcg
     1441 ggggcctgcg gacctcgatg gccaccaccg gctaccagac ggtcaaggag ttccagaagg
     1501 ccgaggtcat ggtcgccccg gcgctccaga ccgagggcaa gctcctgcag cgctcccagg
     1561 gcgttgggat gggctaggcc cggtctactc gtcgtcgccc ttgcggcggc gggtcacgac
     1621 ctggcggtcc cagccccggt ggaccgagca gaaggtgtcg gggttgtagc gcgacagccg
     1681 cgtctcgcag ccgtcggcgg cgcacgtccg ggcctgcccg aacgagcgca cggccgggtc
     1741 ggcgccgggc gcccgccggc gggtcggggc ggggatgggc tcctccggct ttgtcaccgt
     1801 cgcctcctcc accagaaaac aaaagcacca cacacccggc gtccggtgtg tggtgcgagg
     1861 tctgggcacc aaggggcggc gacgcccctc tcgccatcta cggtactcct ccgcaagcgg
     1921 tgcgagcaat ccaccgtcgg agcccgtatc ctgcgcccat gacgaccccg gtcccgatcg
     1981 aggcctcgct ggccgaggtc gaggccgccc gcccggagcc tggtgccctc cgagcgcgtc
     2041 cggcccccgg gcacgacacg gtcctggtgc tcgacttcgg cgcccagtac gcccagctca
     2101 tcgcccgccg catccgcgag tgccacgtct tctccgaggt cgtcccctgc gacgtcgacc
     2161 cggacacggt gcgcgcccac gacgccaagg ccctggtcct ctccggcggg cccaagtcgg
     2221 tctacgagcc gggcgccccc gccctcgacc cgcgcctgct ggagctcggc atccccgtcc
     2281 tcgggctctg ctacggccag caggccatgg ccagggccct gggcggccgg gtcgaggcca
     2341 ccggggtccg ggagtacggc cgcaccaccc tgcgcgccca gcccgagggc agcctcctgt
     2401 tcggcgacct gcccgaagag caggtcgtgt ggatgagcca cggcgacacg gtcaccgccg
     2461 ccccgcccgg ggcccgggtg gtggccagca ccgacgcctc cccggtggcc gggttcgagg
     2521 ac
//
LOCUS       DASSWR010000672         4495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_647900,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000672 DASSWR010000000
VERSION     DASSWR010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647900"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1260
                     /locus_tag="VFO47_11310"
     CDS             556..1260
                     /locus_tag="VFO47_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789323.1"
                     /translation="MRIRLRLRLGAATAVGLGALLLATVLASPALAWHSNVTVNARCL
                     DGQVRVHYTVEAWEKGHEATVDVSYELNGKVVELESDDHFFAPKHNQFSGHFDLPAGT
                     TGTITFTAVAHWKGGEKSSDKGSDDLPPADKCEKSTTTTTSMATTTTMKATTTTEAST
                     TTSTTQPEVAPSSSLATTTTEETAGGAATTTTAGGGQLPFTGASSGPMLLAGLALVGG
                     GAVVLLVSRSKGHATR"
     gene            complement(1330..1749)
                     /locus_tag="VFO47_11315"
     CDS             complement(1330..1749)
                     /locus_tag="VFO47_11315"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET8789324.1"
                     /translation="MKSYDAAAEINASPDAIWAILTDAPGYQAWDSGVERVEGGIAPG
                     EKIKVVSRANPGRAFPVKVTEFAPGRRMTWSGGMLLGLFKGVRTFTLAPGTDGTTRFT
                     MREEYTGPLPPLSWRSMPDLGPSFRQFASGLKERAER"
     gene            complement(1979..3643)
                     /locus_tag="VFO47_11320"
     CDS             complement(1979..3643)
                     /locus_tag="VFO47_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HET8789325.1"
                     /translation="MADGALIVQSDRSVLLEVDHPRAAAARAAIAPFAELEKSPEHVH
                     TYRLTPLALWNARAAGLTPAQVTDALREHSRYPVPPGLLTDLVETMGRYGRLVLRAED
                     GGLVLVSSDPAVLTEVLRSRKVAPHLGERMGPDRVAVPLAERGRLKQALLGLGWPADD
                     QAGYVEGAPLPIELLAAHAGTGERFGLRRYQRESVAAFAAAGSGVIVLPCGAGKTLVG
                     LGVMTAVGARTLILVTSTLAARQWREELLARTDLQPAQVGEYSGARKEVAPVTIATYQ
                     VLVTRRGGRHPHLDLLGREDWGLIVYDEVHLLPAPVFRMTAEIQARRRLGLTATLVRE
                     DGREADVFSLIGPKRYDVPWRDLEAQGWVAAADCVEVRVDLTDEERMDYALAEPEERY
                     RRAATARSKLPVVASLAEAHRADRVLVIGQYLDQLRTLGELLDAPVITGDTPVKEREE
                     LFAAFRTGKLGRLVVSKVANFSIDLPEANVAIQVSGTFGSRQEEAQRLGRILRPKADG
                     GRARFYTVVSRDTNDTEYAQRRQRFLAEQGYAYRLVDATEVLATRA"
     gene            complement(3636..>4495)
                     /locus_tag="VFO47_11325"
     CDS             complement(3636..>4495)
                     /locus_tag="VFO47_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET8789326.1"
                     /translation="TVRAVLDVAAFLGLAVVEGGRAQAGLAVEAWLAGTGAGGLTEQV
                     AEALPDGPDRFLMAGDLTVVAPGGLAPGVETRLAGLADREAAGSGTWRVHDASLRRAF
                     DEGRGADEVLDFLRRHSSTPLPQALEYLVADVARRHGRLRVGGSTTYLRGDPAMVTGA
                     VRSAAGRRLGLRELAPGVAVTGRSQRELLAALRKAGEAPLAEEPDGSPRPEGARPVRH
                     VQRVARDRLDRTAATPNGHGPDRQPDIDPATAVDRLRGRRPAPTGQPIPGDPGSAEAP
                     DPERRVARG"
BASE COUNT          568 a         1670 c         1698 g          559 t
ORIGIN      
        1 gatgcgacgg gcgggtgacc ggtcaggtgg acggtggagt cggtatcccg gtggccggtg
       61 atgtcggcgg agcgcatgcg ggcgtccttt aggtgactgg aacccggcgc tcgaggtggc
      121 cgtgcgacgg cggtgagacc gggcagggaa cggctggttg accggcccag gcgggcggct
      181 cgcctcagcg gaggggcgga gccgggcgcc gggcccgggg acacgcgcag gaccccttca
      241 gcctcggggt gaggcgggga tcgcggtcca gggtggcgtt ccggtgcggc atggcaatgc
      301 gggagggtga cggctccgcc gccggctgtc aagcgcggaa ccgaaccggc cgcctttatc
      361 ggatcgcaac catcccgtgc cgctgtggtt gccggatcaa cctggttgat tggggcgatt
      421 cggctatggt ttcgccacct ttggtgcctg cgtcccctgt cgttcggccc tgccggcacc
      481 cgacaagcgc ccgaccgccc gggtcaccga cgattgccgc cttctcgcgg ttgcatctga
      541 aaggagcacg acagcatgag gatccgtctc cgtctccgtc tcggcgcggc cacggccgtc
      601 ggcctcggag cgctgctcct ggccacggtg ctggcctccc ccgcgctcgc ctggcactcc
      661 aacgtcaccg tcaacgcccg ctgcctcgac ggccaggtcc gtgtccacta cacggtggag
      721 gcctgggaga aggggcacga ggcgaccgtc gacgtctcct acgagctcaa cggcaaggtc
      781 gtcgagctgg agtccgacga ccacttcttc gcgcccaagc acaaccagtt ctccggccac
      841 ttcgatctgc ccgccggcac caccgggacg atcaccttca ccgcggtcgc ccactggaag
      901 ggcggcgaga agtcgagcga caagggcagc gacgacctcc cgccggccga caagtgcgag
      961 aagtcgacca ccaccaccac ctccatggcg accacgacga ccatgaaggc gacgaccacc
     1021 accgaggcgt cgaccacgac cagcaccacc cagcccgagg tcgcgccgtc cagctcgctg
     1081 gccacgacca ccaccgagga gaccgcgggg ggcgcggcca ccaccaccac cgcgggcggg
     1141 ggccagctgc ccttcaccgg ggccagctcc ggtccgatgc tgctggccgg gctcgccctg
     1201 gtcggcggcg gcgccgtggt cctcctggtc agccgctcca agggccacgc gacccggtag
     1261 cccggtcgca gggcttccct gacgaagggc ccccacgatc cgggggccct tcgcgtacgc
     1321 aggcggcggt cagcgctcgg cgcgctcctt gaggccgctg gcgaactgcc ggaaggaggg
     1381 ccccaggtcg ggcatggagc gccagctcag gggcgggagc gggcccgtgt actcctcgcg
     1441 catggtgaac cgggtggtgc cgtcggtccc cggtgccagg gtgaaggtgc gcacaccctt
     1501 gaacaggccc agcagcatgc cgccgctcca ggtcatccgc cggccggggg cgaactcggt
     1561 caccttgacc gggaaggcgc gacccgggtt ggccctggag acgaccttga tcttctcccc
     1621 gggcgcgatg ccgccctcga cccgctcgac gcccgagtcc catgcctggt agccgggagc
     1681 gtcggtcagg atggcccaga tggcgtcggg gctggcgttg atctcggcgg ccgcgtcgta
     1741 cgacttcatc ccgggctgat cccccgccgg cgacgacacc actaccgtca ccatcaccca
     1801 ccgaggctgg gagcgcctcg gcgccgacgg cccgggctgg cgccagcaca acctcggcgg
     1861 ctggggcggc ctgctccccc acgacaagca ggcggcaacc tcacccgccg ctgccgggtc
     1921 ccccggtggg ggggcgtagc cgaccgcggt tgggacctgc gccgggtcgt caacagccct
     1981 aggcccgggt ggccaggacc tcggtggcgt cgaccagccg gtaggcgtag ccctgctcgg
     2041 ccaggaagcg ctggcggcgc tgggcgtact cggtgtcgtt ggtgtcgcgg ctgaccaccg
     2101 tgtagaagcg ggcccggccg ccgtcggcct tggggcggag gatgcggccg aggcgctggg
     2161 cctcctcctg gcggctgccg aaggtgccag agacctggat ggcgacgttg gcctcgggca
     2221 ggtcgatgga gaagttggcc accttggaga cgaccagccg gccgagcttg cccgtccgga
     2281 aggcggcgaa cagctcctcg cgctccttca ccggggtgtc gccggtgatc acgggggcgt
     2341 ccagcagctc gccaagggtc cggagctggt cgaggtactg cccgatgacc agcacccggt
     2401 cggcccggtg ggcctcggcc aggctggcca ccaccgggag cttggagcgg gcggtggccg
     2461 cccggcggta gcgctcctcc ggctcggcca gggcgtagtc catccgctcc tcgtcggtca
     2521 ggtcgacccg gacctcgacg cagtcggcgg ccgccaccca gccctgggcc tccaggtcgc
     2581 gccagggcac gtcgtagcgc ttgggcccga tcaggctgaa cacgtcggcc tcgcggccgt
     2641 cctcgcggac cagggtggcg gtcaggccga gccgccgcct ggcctggatc tcagccgtca
     2701 tgcggaacac gggggccggc agcaggtgga cctcgtcgta gacgatcagc ccccagtcct
     2761 cgcggccgag gaggtccagg tgggggtggc ggccgccgcg ccgggtgacc agcacctggt
     2821 aggtggcgat ggtgaccggg gccacctcct tgcgcgcccc cgagtactcc cccacctggg
     2881 ccggctggag gtcggtgcgg gccagcagct cctcgcgcca ctggcgggcg gccagggtcg
     2941 aggtgaccag gatgagcgtg cgggccccga ccgcggtcat gacaccgagg cccacgaggg
     3001 tcttgccggc accgcagggc agcacgatca cccccgaccc ggcggcggcg aaggcggcca
     3061 ccgactcgcg ctggtagcgg cgcagcccga accgctcgcc ggtgccggcg tgggcggcca
     3121 gcagctcgat cggcagcggc gccccctcga cgtagccggc ctggtcgtcg gcgggccagc
     3181 cgaggccgag cagcgcctgc ttgagccgcc cccgctcggc caggggcacc gcgacccggt
     3241 cgggccccat ccgctcgccc aggtgggggg cgaccttgcg gctgcggagg acctcggtga
     3301 gcacggccgg gtcggaggag acgagcacca ggccgccgtc ctcggcccgc aggaccagcc
     3361 ggccgtagcg ccccatggtc tcgaccaggt cggtgagcag ccccggcggc accgggtagc
     3421 gcgagtgttc gcgcagggcg tcggtgacct gcgccggggt cagcccggcg gccctggcgt
     3481 tccagagggc cagaggggtc agccggtagg tgtggacgtg ctcggggctc ttctccagct
     3541 ccgcgaacgg cgcgatggcc gcccgggccg cggccgcccg tgggtggtcg acctccagca
     3601 gcacgctccg gtcgctctgg acgatcagcg ccccgtcagc cacgcgccac ccgccgttcc
     3661 gggtcggggg cctcagcgga gccggggtcg cccgggatcg gctggccggt cggggccggg
     3721 cggcggccgc ggaggcggtc gacggcggtg gcggggtcga tgtcgggctg gcggtcgggg
     3781 ccgtggccgt tgggggtggc cgccgtccgg tccaggcggt cgcgggcgac ccgctgcaca
     3841 tggcggacgg gccgggcgcc ctcggggcgg gggctgccgt ccggctcctc ggcgaggggg
     3901 gcctctcccg ccttgcgcag ggcggccagc agctcgcgct ggctgcggcc ggtcacggcc
     3961 acgccggggg ccagctcgcg caggcccagg cggcggccgg cggccgagcg gaccgcgccc
     4021 gtgaccatgg ccgggtcgcc gcgcaggtag gtggtggacc cgccgacccg cagccgcccg
     4081 tggcggcggg ccacgtcggc caccaggtac tcgagggcct ggggcagcgg ggtcgaggag
     4141 tggcggcgca ggaagtccag cacctcgtcg gcgccgcggc cctcgtcgaa cgcccgccgc
     4201 aggctggcgt cgtggacgcg ccaggtcccc gacccggccg cctcccggtc ggccagcccg
     4261 gccagccggg tctcgacccc cggcgccagc ccgcccgggg ccaccaccgt caggtccccg
     4321 gccatcagga accggtcggg cccgtcgggc agcgcctccg ccacctgctc ggtcagcccg
     4381 cccgccccgg tcccggcgag ccacgcctcc acggccagcc cggcctgggc ccgtcccccc
     4441 tcgaccaccg cgagcccgag gaaggcggcc acgtccagca ccgccctgac cgtcg
//
LOCUS       DASSWR010000673         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_167571,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000673 DASSWR010000000
VERSION     DASSWR010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167571"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1008
                     /gene="glgB"
                     /locus_tag="VFO47_11330"
                     /pseudo
     CDS             1..1008
                     /gene="glgB"
                     /locus_tag="VFO47_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645077.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
     gene            1005..2720
                     /gene="treZ"
                     /locus_tag="VFO47_11335"
     CDS             1005..2720
                     /gene="treZ"
                     /locus_tag="VFO47_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET8789327.1"
                     /translation="MTPPLGPRLVEGGAEFGVWAPKVRSLAVRVGDRTVALERQERGV
                     WRGRVEGAAAGDDYLLVLDGERERPDPRSRFQPHGVHGPSRLVDLGPPPAPFATPALR
                     ELVFYELHCGTFTPEGTFDAAIGRLGHLAGLGVNAVEVMPVAAFPGSRGWGYDGVALY
                     AVHEAYGGPAAFRRFVDACHEAGLAVVLDVVYNHLGPDGNYLAEFGPYFTDVATTDWG
                     PAVNLDGPGSDFVRGFVLDNAEQWVAGYGVDGLRLDAVQALFDRSSVHILEELSARVH
                     GARPGSVVVAESDLHQPLLVTAYGVDAAWADDLHHALHVALTGETSGWYEGFQDRETL
                     SKALETGWVFTGQYNPYLDRRLGRDPAGLGGERFVVCAQNHDQVGNRPFGDRLAALVG
                     PDLDAVAAVLVACSPFLPLLFQGQEWGSTRPFLYFTDHGPGLAESVRRGRRAEFAAFD
                     WGEEVPDPNDPVTFERSKLDWDRAGGPALELWRALLRLRREVPALGNCRRDLTTARVD
                     PERRLVVLERGDPSGSRAVVVANLGERSQHVPVDTGKLRLRVATRPQSPSGDLSGASA
                     AVWTT"
BASE COUNT          357 a         1013 c          983 g          394 t
ORIGIN      
        1 gccctctact ggctggagga gttccacatc gacggcctgc gggtggacgc ggtcgcctcc
       61 atgctctacc tggactactc gcgcaacgag ggcgagtggg tccccaacga gtacggcggc
      121 aacgaggacc tggacgccgt ccggttcctc aaggacttca acgccgtgac ctaccgggag
      181 cacccggggg cgatgacggt ggccgaggag tcgaccgcct ggccgagcgt gtcacggccc
      241 gtctacctgg gcgggctcgg cttcggcttc aagtggaaca tgggctggat gcacgacacc
      301 ctccagtaca tctccaagga cccgatctaa cgccgcttcc accaccacca gctaaccttc
      361 tcgctgatct acgccttcag cgagaacttc atcctgccca tcagccacga cgaggtcgtc
      421 cacggcaagg gctcgctgct gggcaagatg cccggcgaca cctggcggcg gttcgccaac
      481 ctgcgcgcct acctgggctt catgtgggcc cacccgggca agcagctgat cttcatgggc
      541 tgtgagctgg gccaggaggc cgagtggtcg cacgagcgct cgatcgactg ggagtccctg
      601 gccgacccga accacaaggg cgtccagacc ctggtcggcg acctcaaccg cgtctaccgg
      661 gccaacccgg ccctgtggca gcaggactcc aagccggagg ggttctcctg gatcgacccc
      721 aacgacgtcg acgacaacgc cctcagcttc atccgctggg ccgccgacgg ccggccgctg
      781 gtctgcctct gcaacttctc gccggtgccc cgcagcgagt accggatcgg gctgccgacg
      841 gtcgggcgct ggggcgaggt gctcaacacc gactccgcgg cctacggcgg caccaacgtc
      901 ggcaacatgg gcgtggtcga ggccgaggcg atcgagtggg acggccagcc ggcctcggcc
      961 cgggtcaccc tgccacccct ggccacggtc tggctcgcgc ccgagtgacc cctcccctgg
     1021 ggccccggct ggtcgagggg ggcgccgagt tcggggtgtg ggcgcccaag gtgcgctccc
     1081 tggccgtgcg ggtgggcgac cggaccgtgg cgctggagcg gcaggagcgg ggcgtctggc
     1141 gggggagggt cgaaggggcc gccgccggcg acgactacct gctggtcctc gacggcgagc
     1201 gggagcggcc cgacccgcgc tcgaggttcc agccccacgg cgtccacggc ccgtcgcggc
     1261 tggtcgacct gggcccgccg ccggccccgt tcgccacccc ggccctccgc gagctggtct
     1321 tctacgagct gcactgtggc accttcaccc ccgagggcac cttcgacgcg gccatcgggc
     1381 ggctcggcca cctggccggg ctgggcgtga acgcggtcga ggtcatgccg gtggccgcgt
     1441 tcccggggtc gcggggctgg ggctacgacg gggtcgcgct gtacgccgtg cacgaggcct
     1501 acggcgggcc ggccgccttc cgccgcttcg tcgacgcctg ccacgaggcc gggctggccg
     1561 tggtcctgga cgtcgtctac aaccacctcg gcccggacgg caactacctg gccgagttcg
     1621 gcccctactt caccgacgtg gccaccaccg actggggccc ggcggtcaac ctggacgggc
     1681 ccgggtcgga cttcgtgcgc gggttcgtgc tcgacaacgc cgagcagtgg gtggccggct
     1741 acggcgtcga cggccttcgc ctggacgccg tccaggccct gttcgaccgc agctcggtgc
     1801 acatcctgga ggagctgtcg gcccgggtcc acggcgcccg gcccggctcg gtggtggtcg
     1861 ccgagagcga cctgcaccag ccgctgctgg tcaccgcgta cggggtcgac gccgcctggg
     1921 ccgacgacct ccaccatgcc ctccacgtcg ccctcaccgg cgagacctcc ggctggtacg
     1981 agggcttcca ggaccgcgag acgctctcca aggcgctgga gaccggctgg gtgttcaccg
     2041 gccagtacaa cccctacctg gaccgccgcc tgggccgcga cccggccggg ctgggcgggg
     2101 agcggttcgt ggtctgcgcc cagaaccacg accaggtcgg caaccgcccc ttcggagacc
     2161 ggctggccgc cctggtcggc cccgacctgg atgccgtggc cgccgtgctg gtcgcctgct
     2221 cgccgttcct gcccctgctg ttccagggcc aggagtgggg ctctacgcgc ccgttcctct
     2281 acttcaccga ccatgggccg ggcctggccg agtcggtccg gcggggccgg cgggccgagt
     2341 tcgccgcctt cgactggggc gaggaggtgc ccgatcccaa cgacccggtc accttcgagc
     2401 ggtccaagct cgactgggac cgggccggcg gcccggccct ggagctgtgg cgggccctgc
     2461 tgcggctgcg ccgcgaggtg ccggcgctcg ggaactgccg ccgcgacctg accaccgccc
     2521 gggtcgaccc ggagcggcgg ctggtcgtgc tggagcgggg cgacccgtcg gggtcgcggg
     2581 cggtcgtcgt cgccaacctg ggcgaaaggt cccagcatgt ccccgtcgac acgggtaagc
     2641 tgcggctccg ggtcgcgacc cgcccgcaat cgcccagcgg cgacctatct ggcgcgagcg
     2701 cagcggtatg gacgacttga cgggtttgac gccggcctag cgggccg
//
LOCUS       DASSWR010000674         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1014654, whole genome shotgun sequence.
ACCESSION   DASSWR010000674 DASSWR010000000
VERSION     DASSWR010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014654"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFO47_11340"
     CDS             complement(<1..947)
                     /locus_tag="VFO47_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8789328.1"
                     /translation="MLVAVALLVFAAGRYGHGISLTVVDTWVLARLADVRSPGLTGAM
                     AAVTGLLGSLGTVKAVSLATLLVLVACRRFRHLLVAWASLQVVTLLVLGLSILVRRPR
                     PFGVPIESDWAGWSMPSRPVAYLAAILLVMLYSLVPKGRWRQAGKWIATVLVALLGLA
                     HMYLAVDHPSDVLVAAALGVTIPLLAFRWFTPNEVFPVTYRRGSRAHLDVGGARGAAI
                     RRALSDQLGLEVEEVKPFGLAGSAGSTPLKITVKGDPETTLFGKLYARSHLRSDRWYK
                     LGRELLYGRLEDEKPFHTVRRLVQQEDYALHKLHAAGLPT"
     gene            complement(1457..>2245)
                     /locus_tag="VFO47_11345"
     CDS             complement(1457..>2245)
                     /locus_tag="VFO47_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HET8789329.1"
                     /translation="QAFCHRDLSDRDYVYVWADGIHFRVRLEQARLCCLVIVGVRADG
                     TKELVAVADGERESTDSWAEVLRDLRRRGMRAPVVAVGDGALGLWAALRDVFPATRQQ
                     RDWVHKLANVLGCLPAAVQAGARKALVEIRDAPDRDHAVRAIEVFARDYGVKWPKAVA
                     KITDDAEELLCFYDFPAEHWLHLKTSNPIESTFSSVRLRTRVTKGPGSKAAGLAMTFK
                     LLEAAQDRWRAVNGPHLVALVRAGARFDKGVMIQRPDELQEDAA"
BASE COUNT          368 a          785 c          783 g          309 t
ORIGIN      
        1 gtgggcagcc cggcagcgtg cagcttgtgc agggcgtagt cctcctgctg gaccagccgg
       61 cggacggtgt ggaagggctt ctcgtcctcc aggcggccat agagcagctc ccggcccagc
      121 ttgtaccagc ggtccgagcg caggtggctg cgggcataca gcttgccgaa cagtgtggtc
      181 tcgggatcgc ccttgacggt gatctttagc ggggtggagc cggccgagcc ggccagcccg
      241 aacggcttca cctcctcgac ctccaggccg agctggtccg agagggcccg ccggatggcc
      301 gcccctctgg ccccgccgac gtccaggtgg gcgcggctgc cccgccggta ggtgaccggg
      361 aagacctcgt tgggggtgaa ccagcggaag gccagcaggg ggatggtcac cccgagtgcg
      421 gcggcgacca gcacgtcgct ggggtggtcg acagccagat acatgtgggc caggccgagc
      481 agcgccacca ggacggtggc gatccacttg ccggcctggc gccagcggcc cttggggacc
      541 aggctgtaga gcatgacgag gaggatggcg gccaggtagg cgaccggccg tgacggcatc
      601 gaccagccgg cccagtcact ctcgatgggc accccgaacg gccgcggccg gcggaccagg
      661 atcgacagac caaggaccag cagggtaaca acctgcagtg aggcccaggc gaccagcagg
      721 tgccggaagc gcctgcaggc aaccagcacc agcaaggttg ccaagcttac agccttgacc
      781 gtccccagcg agcccagcag ccccgtcacg gccgccatcg ctccggtcag cccggggctt
      841 cgtacgtcgg ccagccgcgc cagcacccag gtgtcaacaa cggtcaggga gatcccgtgg
      901 ccgtagcggc cagcggcaaa taccagcagc gccaccgcca ccagcaccac cgcggccagg
      961 agccagccga ccccggtgct ctccaggtgg cggggcagtg gtggcggctc cccggtcgga
     1021 cgccgtcgac gccccgactg ccagctgccc ggcggggtcg caatcggggt cgatgccctc
     1081 gcaggcccgg ccgtccctcg cccggtcctg accgcatgga ctggactggt cttcattacc
     1141 acttccaacc ggacgacaca tgcacggcgc gtcgtaacca taggcctgaa cgattgagcg
     1201 caagagggtc caaagtcctg tcgcccacag gacccgcgac ccagccgtcg gcctagcaag
     1261 cttcggtgag gggctggtcg aggccaggag tgcagccctg gtgtgagcgg acggcctgca
     1321 aacgatgggg ccatcggccc taccggttgg tggcgtggcg gtgcttcttg aagcgtgatg
     1381 aggtcgtgag cgttgggggc cgcggagatg tggcaggagg aatcgtcaag agttgtggat
     1441 cgcgcgtgct gcgtgattac gcggcgtcct cctggagctc gtcagggcgc tggatcatta
     1501 cccccttgtc gaagcgggcg ccggcgcgca ccagcgcgac caggtggggg ccgttgacgg
     1561 cgcgccagcg gtcctgggcg gcctccagca gcttgaaggt catcgccagc ccagcggcct
     1621 tggagccggg ccccttggtc acgcgggttc gcagcctgac cgacgagaag gtcgactcga
     1681 tcgggttgct ggtcttcaga tgcagccagt gctcggccgg gaagtcgtaa aagcacagca
     1741 gttcctcggc gtcgtcggtg atcttggcca cggccttggg ccacttgacc ccgtagtcgc
     1801 gggcgaacac ctcaatcgca cgaacggcgt ggtcccggtc gggggcatcc cgaatctcaa
     1861 ccagggcctt acgcgcgccg gcctgcaccg cggcgggcag acagcccagc acgttggcga
     1921 gcttgtggac ccagtcgcgc tgctggcggg tggccgggaa cacgtcccgc agcgccgccc
     1981 acaaccccag cgcgccgtcc ccgaccgcca ccaccggcgc ccgcatcccg cgccgccgta
     2041 ggtcccgcag gacctccgcc cagctgtcgg tcgactcgcg ctccccgtca gccaccgcga
     2101 ccagctcctt ggtgccatcg gcgcgcacgc ccacgatcac caggcagcac agccgagcct
     2161 gctccagccg cacccggaag tggatgccgt cggcccagac gtacacgtag tcacggtcgg
     2221 acaagtcacg gtggcagaac gcctg
//
LOCUS       DASSWR010000675         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1042915, whole genome shotgun sequence.
ACCESSION   DASSWR010000675 DASSWR010000000
VERSION     DASSWR010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042915"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..826)
                     /locus_tag="VFO47_11350"
     CDS             complement(41..826)
                     /locus_tag="VFO47_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8789330.1"
                     /translation="MREQALVTGASSGIGLELARQFVQHGFDVIITAEDDELATAEAA
                     LAGDGAEVRAVKADLSTSDGVERLWAAVAGLGRPLDAVALNAGIGVNGDFTRDIPLED
                     DLRLIAVNVTAVVHLAKRILPGMVDRGEGRVLITSSVAATAPGPYYATYAASKAFLQS
                     FAQAVRHELKDSGVTVTALQPGPTDTEFFDRAGMDDTKVAEAKKDDPAEVAKDGFEAL
                     MAGKDHVIAGSVKNKAQVVGGKVLSEKARAATQAAQTKPQADE"
     gene            957..1598
                     /locus_tag="VFO47_11355"
     CDS             957..1598
                     /locus_tag="VFO47_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8789331.1"
                     /translation="MEAIVTVTRTARANPTYYGGDLRRDLLGAALELIDREGPSAVSL
                     RSLARRLGVSHAAPANHFPDKAALFTAIAVEGFELLSAAITTATREAGPDATPGQRLR
                     AAGRAYTGFAVGHPAHFAVMWQRDLLHQDDPALCAAGEATFALLLAGVRDVQDAGWAA
                     GTDERTVAYLAWSVVHGLAALWLGGSLAQDQRPFDEIAGEVGALLGRALAPDA"
     gene            1619..2050
                     /locus_tag="VFO47_11360"
     CDS             1619..2050
                     /locus_tag="VFO47_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET8789332.1"
                     /translation="MHAHHQHRPARTLPRPADPRLRVGDTERGQVIDQLADHHAAGRL
                     TLEEFEERMASAWTARTGADLEVLVRDLPVPPRPPQPSRRPAAAPRPGLDSPVRTYLA
                     VMALLWLIWLVTGANYPWPIWPMLGWGIGVAGHLGRPRTAR"
BASE COUNT          263 a          863 c          792 g          295 t
ORIGIN      
        1 gtcccccgcg acagcccgcc gcgtcaccgg gcgggttgcc ctactcgtct gcttggggct
       61 tggtctgggc ggcctgcgtt gccgccctgg ccttctcgga caggaccttg cccccgacca
      121 cctgggcctt gttcttgacg ctgccggcga tgacgtggtc cttgccggcc atcagggcct
      181 cgaacccgtc cttggcgacc tcggccgggt cgtccttctt ggcctcggcg accttggtgt
      241 cgtccatgcc ggcccggtcg aagaactcgg tgtcggtcgg ccccggctgg agcgcggtca
      301 cggtcacgcc gctgtccttc agctcgtgcc ggaccgcctg ggcgaacgac tggaggaagg
      361 ccttggaggc ggcgtaggtg gcgtagtagg ggccgggggc ggtcgcggcc accgaggagg
      421 tgatcagcac ccgcccctcg ccccggtcga ccatgccggg caggatccgc ttggccaggt
      481 gcacgaccgc ggtgacgttg acggcgatca gccgcaggtc gtcctccaag gggatgtcgc
      541 gggtgaagtc gccgttgacg ccgatcccgg cgttgagggc caccgcgtcc agcggccgcc
      601 cgagcccggc cacggccgcc cagagccgct ccaccccgtc ggaggtcgac aggtcggcct
      661 tgaccgcccg gacctcggcg ccgtcgccgg ccagggccgc ctcggccgtc gccagctcgt
      721 cgtcctcggc ggtgatgatg acgtcgaagc cgtgctggac gaactggcgc gccagctcca
      781 gcccgatccc gctcgacgcc ccggtgacca gggcctgttc ccgcatgtcg gcaccacgtc
      841 cttgtctcgg gaggtccgtc cgccaggcgt taccacgccc gcccggcccg gtaacgcccg
      901 ccagcaggtt gacactgcca acctgaagga ctagattgac actgccaatc tgaaggatgg
      961 aggcaatcgt gacggtgacc cggacggcca gggcgaaccc gacctactac ggcggcgacc
     1021 tccgccggga cctgctgggc gcggccctgg agctgatcga ccgggagggg ccgtcggcgg
     1081 tgagcctgcg gtcgctggcc cggcggctcg gcgtctcaca tgccgccccg gccaaccact
     1141 tccccgacaa ggcggccctg ttcaccgcca tcgccgtcga gggcttcgag ctgctgtcgg
     1201 cggccatcac caccgccacc cgggaggccg gcccggacgc gaccccgggc cagcggctgc
     1261 gcgccgccgg ccgggcctac accggctttg ccgtcggcca cccggcccac ttcgcggtga
     1321 tgtggcagcg cgacctgctc caccaggacg acccggcgct gtgcgcggcc ggcgaggcca
     1381 ccttcgccct gctgctcgcc ggggtccgcg acgtccagga tgccggctgg gcggccggca
     1441 ccgacgagcg gaccgtcgcc tacctggcct ggtcggtggt gcacgggctg gccgccctgt
     1501 ggctgggcgg atcgctcgcc caggaccagc gcccgttcga cgagatcgcc ggcgaggtcg
     1561 gcgccctgct cggccgcgcc ctcgcccccg acgcctgacc gacccggagg agaccaccat
     1621 gcacgcccac caccagcacc gcccggcccg gacgctgccc cggccggccg acccccggct
     1681 gcgggtcggt gacaccgagc gcggccaggt gatcgaccag ctggccgacc accacgccgc
     1741 cgggcgcctc accctggagg agttcgagga gcggatggcc tcggcctgga ccgcccggac
     1801 cggcgccgac ctggaggtcc tggtccgcga cctgcccgtc ccgccgcggc cgccgcagcc
     1861 gtcgcgccgg ccggcggccg ccccccggcc cggccttgac agcccggtcc ggacctacct
     1921 ggccgtgatg gccctgctgt ggctgatctg gctggtcacc ggggcgaact acccctggcc
     1981 catctggccc atgctcggct ggggcatcgg cgtcgccggc cacctcggcc gcccccggac
     2041 ggcccgctga accggcggct caaccgtcgg cggccaggaa cgcgagcagc tcggcggcca
     2101 gggcgtcggg ggcctcctcg gccatgtggt ggccggaggg gaacggccgg ccccgcaggt
     2161 cggggcccag gggcgccaga cggcgaggac gtcgccgtag aggtcgccca ggt
//
LOCUS       DASSWR010000676         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_223084,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000676 DASSWR010000000
VERSION     DASSWR010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223084"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFO47_11365"
     CDS             <1..818
                     /locus_tag="VFO47_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018783792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-5-chlorophenol 1,6-dioxygenase subunit
                     alpha"
                     /protein_id="HET8789333.1"
                     /translation="VVGAALVPGMPHLLAAEPAPSWKLLADGARQAGRELRAAGPDAF
                     VVLSTQWFTVLGHQFQVDPNPRGGHVDENWYAYDYGTLDYDLRVDVELAQAWACQAED
                     LGFQARRTRYDGFPIDTGTVVAGRLVDPGRTRPIALVSCNLYAAAGDLGRIAEAAARA
                     AGELGRRVAFVAVSGLSSGLIQRWIEPAEDRFEDAGHDAWNRRVLDLLAAGRLDEALA
                     LREHYAREAQADSQFRALAFLAGSGACDRQARVLEYGPVWGTGAAVVTWLDGS"
     gene            842..1135
                     /locus_tag="VFO47_11370"
     CDS             842..1135
                     /locus_tag="VFO47_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018783793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET8789334.1"
                     /translation="MANEAAGFIEAEGFVPIFDAIDTMVKATEVRVNGVMRLGGGLVA
                     VALGGDLATVEEAVEIGEETAKAVSRGKVKSIIFASPSRPVAAIAGNPRLLDG"
     gene            1146..1484
                     /locus_tag="VFO47_11375"
     CDS             1146..1484
                     /locus_tag="VFO47_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET8789335.1"
                     /translation="MPSERAIGLFETRGVVGLTAGIEAMMKTADVECVAVERVSSGYF
                     AAAIQGSIAAVRQAMEAGSEAVRRYGDLRSAQIYPKPHEVASALLANGTAEQLSGRAI
                     ESGKKSRGSA"
     gene            1484..1792
                     /locus_tag="VFO47_11380"
     CDS             1484..1792
                     /locus_tag="VFO47_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET8789336.1"
                     /translation="MLLAEVLGRVWAERQVPDLDGRRMVLVRELSTSAAHVAVDLVDV
                     AAGNTVLVVTDDAAQTATGGAAIDAAVVALVAGTDRLPTPIASPTARRSERRAERTRP
                     "
     gene            1789..3618
                     /locus_tag="VFO47_11385"
     CDS             1789..3618
                     /locus_tag="VFO47_11385"
                     /inference="COORDINATES: protein motif:HMM:NF006150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET8789337.1"
                     /translation="MSQDRFPSPFEVPTPEGAEGWEQLYNWYHLFGEDRRDLDEQRFW
                     FQDRLHHPGVLHPYDEIQCECWWQALGAFNTRIFAMPPAFGVDQRILNGYLYVTPVPA
                     PPESVPERAELFGRRGGHYYEHWDQIYAEWKDKVTAHLEEIKAIRFAPLPDLEDESVV
                     FDHVGYSSGYRMIEDFSKLVLTMYKTYQYHFELLNVGYAAYLTFFMFAKQAFPDITDQ
                     SISRMVGGLHVDLYRPDDELKRLAKEAERLGVGEAVLAAGDPASLFEQLRQGEGGREW
                     VADWERTSDPWFLFNTDPGHPGGYHYYDTWLDDPGIPLASVKEYVRRLRAGEALDRPT
                     DQVLAERDRITGEYRALLAEGDQGAFDEMVALARKVFVYIEEHVLYIEHWIWATFWGK
                     SKELSRALAAMGIFDDPEDMFFLRRYEVMEALYDTVAAWSVGSRARGADYWRPIVTER
                     RRIHEALSAADAVPALGPPPAEVSEPFTVMLWGITTDTVGEWLREGQGDGDGQATLRG
                     VAGSPGVVEGPARVVRHVSQLDEVQEGEILVCPATSPSWSPVFSRIAATVSDVGGIMS
                     HTAIICREYGLPAVIGTGKAVATIKTGQRIRVDGSEGVVTLLG"
BASE COUNT          520 a         1374 c         1363 g          507 t
ORIGIN      
        1 ccgtcgtcgg ggccgccctg gtcccgggga tgccgcacct gctggcggcc gagccggcgc
       61 cgagctggaa gctgctggcc gacggggccc ggcaggccgg ccgggagctg cgggcggccg
      121 gcccggacgc cttcgtggtc ctgtcgaccc agtggttcac cgtcctcggg caccagttcc
      181 aggtcgaccc caacccccgc ggcggccacg tcgacgagaa ctggtacgcc tacgactacg
      241 gcaccctcga ctacgacctg cgggtcgacg tcgagctggc ccaggcctgg gcctgccagg
      301 ccgaggacct gggcttccag gcccgccgga cccgctacga cggcttcccg atcgacaccg
      361 gcacggtggt cgcgggccgg ctggtcgacc ccggccgcac ccgcccgatc gcgctcgtct
      421 cctgcaacct gtacgcggcc gccggcgacc tgggccggat cgccgaggcg gcggcccggg
      481 cggccggcga gctcggccgg cgggtcgcct tcgtggccgt ctccggcctg tcgtcggggc
      541 tgatccagcg ctggatcgag ccggccgagg accgcttcga ggacgccggc cacgacgcct
      601 ggaaccgccg cgtgctcgac ctgctggcgg ccggccgcct cgacgaggcc ctggccctgc
      661 gcgagcacta cgcccgggag gcgcaggccg acagccagtt ccgggccctg gccttcctgg
      721 ccggcagcgg cgcctgcgac cgccaggcca gggtgctcga gtacgggccg gtctggggca
      781 ccggggcggc cgtggtcacc tggctggacg gcagctgagg gacacgagag gggagagcgg
      841 catggccaac gaggcggccg ggttcatcga ggccgagggg ttcgtcccca tcttcgacgc
      901 catcgacacg atggtgaagg cgaccgaggt gcgcgtgaac ggggtgatgc gcctgggcgg
      961 cgggctggtc gcggtcgccc ttgggggtga cctggccacc gtcgaggagg cggtggagat
     1021 cggggaggag acggccaagg ccgtctcccg gggcaaggtc aagtcgatca tcttcgccag
     1081 cccgagccgg ccggtcgcgg ccatcgccgg caacccgcgg ctgctggacg gataggaggg
     1141 cagcgatgcc gtcggagcgt gccatcggcc tgttcgagac ccgcggagtg gtcgggctca
     1201 cggccgggat cgaggccatg atgaagaccg ccgacgtcga gtgcgtcgcc gtcgagcggg
     1261 tgtccagcgg ctacttcgcc gccgccatcc agggcagcat cgccgccgtc cgccaggcca
     1321 tggaggccgg gtcggaggcg gtccgccgct acggcgacct gcgctcggcc cagatctacc
     1381 ccaagccgca cgaggtcgcc tcggccctgc tggccaacgg caccgccgag cagctctcgg
     1441 gccgggcgat cgagtccggc aagaagtccc gcgggagcgc ctgatgctgc tggccgaggt
     1501 cctcggccgc gtctgggccg agcgccaggt gcccgacctg gacggccgcc ggatggtgct
     1561 ggtccgggag ctgtccacct cggccgcgca cgtggccgtc gacctggtcg acgtggccgc
     1621 cggcaacacc gtgctggtcg tcaccgacga cgccgcccag accgccaccg gcggcgcggc
     1681 catcgacgcg gccgtggtcg ccctggtcgc cggcaccgac cggctcccga ccccgatcgc
     1741 ctcccccacg gcccgccgca gcgagcgccg agcggaaagg acacgcccgt gagccaggac
     1801 cgcttcccca gcccgttcga ggtgccgacc ccggagggag ccgagggctg ggagcagctg
     1861 tacaactggt accacctgtt cggcgaggac cggcgcgacc tggacgagca gcggttctgg
     1921 ttccaggacc ggctgcacca cccgggggtg ctccacccct acgacgagat ccagtgcgag
     1981 tgctggtggc aggccctcgg cgcgttcaac acccgcatct tcgccatgcc gcccgccttc
     2041 ggggtcgacc agcgcatcct caacggctac ctgtacgtga ccccggtgcc ggccccgccc
     2101 gagagcgtgc ccgagcgggc cgagctgttc ggccgccgcg gcggccacta ctacgaacac
     2161 tgggaccaga tctacgccga gtggaaggac aaggtcaccg cccacctgga ggagatcaag
     2221 gcgatccggt tcgcgcccct gcccgacctg gaggacgagt cggtggtgtt cgaccacgtc
     2281 ggctactcgt ccgggtaccg gatgatcgag gacttctcca agctcgtcct caccatgtac
     2341 aagacctacc agtaccactt cgagctgctc aacgtcggct acgccgccta cctgaccttc
     2401 ttcatgttcg ccaagcaggc gttccccgac atcaccgacc agtcgatctc acgcatggtg
     2461 ggcggcctcc acgtcgacct gtaccggccc gacgacgagc tcaagcgcct ggccaaggag
     2521 gccgagcgac tcggggtggg cgaggccgtc ctggccgccg gcgacccggc gtcgctgttc
     2581 gagcagctcc ggcagggcga gggcggccgc gagtgggtgg ccgactggga gcggacctcg
     2641 gacccgtggt tcctgttcaa caccgacccc gggcacccgg gtggctacca ctactacgac
     2701 acctggctgg acgaccccgg catcccgctg gcctcggtca aggagtacgt gcgccggctg
     2761 cgggccggcg aggccctcga ccgcccgacc gaccaggtcc tggccgagcg cgaccggatc
     2821 accggcgagt accgggcgct gctggccgag ggcgaccagg gcgccttcga cgagatggtc
     2881 gccctggccc gcaaggtgtt cgtctacatc gaggagcacg tgctctacat cgagcactgg
     2941 atatgggcga cgttctgggg caagtccaag gagctgtcgc gggcgctggc cgccatgggc
     3001 atcttcgacg accccgagga catgttcttc ctgcgccgct acgaggtcat ggaggcgctg
     3061 tacgacaccg tggccgcctg gtcggtgggg agcagggccc gcggggccga ctactggcgg
     3121 ccgatcgtga ccgagcgccg ccgtatccac gaggccctgt cggcggccga cgccgttccc
     3181 gcccttgggc cgccgccggc cgaggtgagc gagccgttca cggtcatgct gtggggcatc
     3241 accaccgaca ccgtcggcga gtggctccgg gaaggccagg gcgacggcga cggccaggcg
     3301 accctgcgcg gggtcgcggg ttcgcccggc gtggtcgagg gcccggcccg ggtcgttcgc
     3361 cacgtgtccc agctcgacga ggtccaggag ggcgagatcc tcgtctgccc ggccacctcc
     3421 ccgtcgtggt cgccggtgtt cagccgcatc gccgccaccg tctccgacgt cggcggcatc
     3481 atgagccaca ccgccatcat ctgccgcgag tacggcctcc cggcggtgat cggcaccggc
     3541 aaggccgtcg ccaccatcaa gaccggccag cgcatccgtg tcgacggcag cgagggcgtc
     3601 gtcacgctgc tcgggtagtt cggcggggcc tgtggatagg gaagggacct ccgcttcccg
     3661 gtcggctacc ctcccggatg gggctcccgc gaccggggtc gagggtgcgt ggtgcgccct
     3721 cgaaccaggc tccgcgccgg cggcgccggt cgatcccgac aact
//
LOCUS       DASSWR010000677         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_421165,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000677 DASSWR010000000
VERSION     DASSWR010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421165"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFO47_11390"
     CDS             complement(<1..827)
                     /locus_tag="VFO47_11390"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HET8789338.1"
                     /translation="MIPNSQAASRRWLAAWTLVRKLPEPVAHALGGVGGRVSLALDRD
                     RREALTANLAQVLGPATGPRELRRAVRQGFASYGRYWVEAFRLEDLTAADLRQRLRIE
                     GREHIDTALAGGRGAIFASPHIGNWDAGGAWLAAVGYPATAVVERLKPAELYERFAVY
                     RRALGMELLPLDDGSETLRGVVRALRAGRLACLVCDRDLTGGGLEVRLFGARAVMPGG
                     PASIALRTGAPLLPCSVYHDRRPGHWRAVVHPPLVPERSGDTRNDTVALTQRLADEF"
     gene            complement(824..1420)
                     /locus_tag="VFO47_11395"
     CDS             complement(824..1420)
                     /locus_tag="VFO47_11395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET8789339.1"
                     /translation="MLSSKARDTFRHVTEPAGHALAKAGITANGLTAIGLAGSLGAGA
                     LIATGQPVIGGAVSLLSGLPDMLDGAVAKASGRVSRKGAFLDSVVDRLSDAAVLSGII
                     WFAAVRDLDAMAVLAALVLGLSLIVSYIKARAESLGFACNVGIAERPERVIVLGLALL
                     LGHAEAGLWVLVAGTAITVVQRLLVVWQQSDVGPDIGR"
     gene            1580..>2346
                     /locus_tag="VFO47_11400"
     CDS             1580..>2346
                     /locus_tag="VFO47_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8789340.1"
                     /translation="MKSYPTDHVRNILIVGHGGAGKTSLVEAVCHATGATNRIGRVED
                     GTTVTDFEPEETRKQISVSLAVAPVEYDGFKINLLDAPGYADFVGDVRSALPAADGVL
                     FVVSAVEGVETQTEVVWTMAEELGLPRAFFINKLDRDRASFARSLDGIQAAFGKACAP
                     LYLPIGEEHDFGGLVGLLSGRAFTYENGKRSEGDVPADLTDAAASLREQLIEAIIQES
                     EDEELMDRYLGGEEIAPTDLIGDLEQAVAAGRLFPVLA"
BASE COUNT          339 a          818 c          905 g          284 t
ORIGIN      
        1 tcgaactcgt cggccagccg ttgggtgagg gcgacggtgt cgttgcgggt gtcgccggag
       61 cgctcgggca ccagcggcgg gtggacgacg gcccgccagt gcccgggacg gcggtcgtgg
      121 tagacgctgc agggcagcag cggcgccccg gtccgcaggg cgatcgaggc cggcccgccc
      181 ggcatcaccg cccgggcccc gaacagccgc acctccaggc cgcccccggt caggtcgcgg
      241 tcgcagacca ggcaggccag gcggccggcg cgcagggccc tgacgacccc gcggagggtc
      301 tcgctgccgt cgtccagcgg cagcagctcc atcccgaggg cccgccggta gacggcgaag
      361 cgctcgtaca gctcggccgg cttcagccgc tcgaccacgg cggtggccgg gtagccgacg
      421 gcggccagcc aggccccgcc ggcgtcccag ttgccgatgt gggggctggc gaagatggcg
      481 ccccggccgc cggccagggc ggtgtcgatg tgctcgcggc cctcgatgcg cagccgctgg
      541 cggaggtcgg cggccgtcag gtcctccagc cggaacgcct ccacccagta gcggccgtag
      601 ctggcgaagc cctgccggac cgcccgccgc agctcgcggg ggccggtggc ggggccgagg
      661 acctgggcca ggttggccgt cagggcctcg cggcggtcac ggtccagggc cagggagacc
      721 cgcccgccga ccccgccgag ggcgtgggcc accggctcgg ggagcttgcg gaccagcgtc
      781 caggcggcca gccagcggcg agaggccgcc tggctgttgg ggatcacctg ccgatgtccg
      841 gcccgacgtc ggattgctgc cagacgacca ggaggcgctg gaccacggtg atggcggtgc
      901 cggcgaccag cacccacagg ccggcctcgg catgcccaag cagcagggcg agcccgagga
      961 cgatgacccg ctcggggcgc tcggcgatgc cgacgttgca ggcgaagccg agcgactcgg
     1021 cccgggcctt gatgtaggag acgatcagcg acaggccgag cacgagggcg gccagcacgg
     1081 ccatggcgtc gaggtcgcgg acggcggcga accagatgat gcccgacagc acggccgcgt
     1141 ccgacagccg gtcgacgacc gagtccagga aggcaccctt gcgcgagacc cggcccgagg
     1201 ccttggccac ggccccgtcg agcatgtcgg ggaggccgga caggaggctg acggcgccgc
     1261 cgatcacggg ctggccggtg gcgatgaggg cgccggcgcc gaggctgccg gcgaggccga
     1321 tggcggtgag gccgttggcg gtgatgccgg ccttggccag ggcgtggccg gccggctcgg
     1381 tgacgtgacg gaaggtgtcg cgggccttgg acgagagcac gagggatacc tgtcgtgacg
     1441 ggggtgttcg tgggggaaca cccaaggttg aacttgggga ggctagcact cctgtagacc
     1501 cggctcaacg gcgaccggtt acggcaatat gcggtggtag catcgccgct ccggcgacat
     1561 cgagggaggc tggccgcctg tgaagagcta ccccaccgac catgtccgca acatcctgat
     1621 cgttggtcac gggggcgccg gcaagacctc gctggtcgag gcggtgtgcc acgccaccgg
     1681 ggccaccaac cgcatcggcc gggtcgagga cgggaccacc gtcaccgact tcgagcccga
     1741 ggagacgcgc aagcagattt cggtctccct ggccgtcgcc ccggtcgagt acgacggctt
     1801 caagatcaac ctgctggacg cccccggcta cgccgacttc gtgggcgacg tgcgctcggc
     1861 cctgcccgcc gccgacgggg tcctgttcgt ggtctcggcc gtcgaggggg tcgagaccca
     1921 gaccgaggtc gtctggacga tggccgagga gctggggctg ccccgggcct ttttcatcaa
     1981 caagctggac cgggaccggg cgtcgttcgc ccgcagcctc gacggcatcc aggccgcctt
     2041 cggcaaggcc tgcgcgccgc tgtacctgcc catcggcgag gagcacgact tcggcggcct
     2101 ggtcggcctg ctgtcgggcc gcgccttcac ctatgagaac ggcaagcgca gcgagggcga
     2161 cgtccccgcc gacctgaccg acgccgcggc cagcctgcgc gagcagctca tcgaggcgat
     2221 catccaggag tccgaggacg aggagctgat ggaccgctac ctcggcgggg aggagatcgc
     2281 ccccaccgac ctgatcggcg acctggagca ggcggtggcc gccgggcggc tgttcccggt
     2341 gctggc
//
LOCUS       DASSWR010000678         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1014729, whole genome shotgun sequence.
ACCESSION   DASSWR010000678 DASSWR010000000
VERSION     DASSWR010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014729"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..857
                     /gene="phbB"
                     /locus_tag="VFO47_11405"
     CDS             123..857
                     /gene="phbB"
                     /locus_tag="VFO47_11405"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HET8789341.1"
                     /translation="MASMEGQVALVTGGARGIGQAISTRLADRGVRVAVGYSRGADTA
                     KAFAAAHEGATIHQGNIGVAADCQRVINEVLDQHGQLDILVNNAGITIDKTVRKMSVE
                     DWDRVVGINLSGAFYLSRAVLQHMLDRGYGRIINISSVIGEKGNIGQANYAAAKSGLF
                     GLTNSLALETARKGITVNSVAPGFIATEMVSAMPEDVLAKVVAQIPVGRLGQPQEIAR
                     VVEFLADPESGYITGEIFDVNGGLYM"
     gene            887..2077
                     /locus_tag="VFO47_11410"
     CDS             887..2077
                     /locus_tag="VFO47_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HET8789342.1"
                     /translation="MAAEREVVVLSGVRTAVGKYGGSLKDMPPADLGAAVVREAVSRS
                     GAEPADVGHVVFGNVIHTETRDMYLSRVAAVNGGLPVETPAFTLNRLCGSGLQAIVSA
                     AQLVLLGDTEVALAGGAESMSRGLYGVPAMRWGARMGETSASDMMVGALTDPFDDCHM
                     GVTAENVAGKWGISREDQDALAVQSHQRAAKATAEGYFKDQILPIELKSRKGTTTFDT
                     DEHIRADATIEGMAKLRPVFDKSGSVTAGNAAGINDAAAAVVLADAGWAERKGLAPMG
                     RLVAYAHAGVEPKYMGIGPVPAVRMVLEKANLKVDDIDVFELNEAFAAQALAVVRDLS
                     LPEDRTNPNGSGVSLGHPIGATGCILTVKALYELERTGGRRALVTMCIGGGQGIAAIF
                     ERAA"
     gene            complement(2428..>2928)
                     /locus_tag="VFO47_11415"
     CDS             complement(2428..>2928)
                     /locus_tag="VFO47_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase"
                     /protein_id="HET8789343.1"
                     /translation="FDILAWNADSTRMPAAMHSFYLRTCYLDNQLARGDMELAGVRLH
                     LDAVTQDLYLVGAANDHITPWQSSYSTTQLVKGDTRFVLSSSGHIAGIVNPPSPKARH
                     WTNDDIPPDPDVWLAGATEHAGSWWDDWVPWAKAHGGQRRKPPPMGNAKHPPIADAPG
                     SYIHEK"
BASE COUNT          431 a          981 c         1105 g          411 t
ORIGIN      
        1 ccacctacaa cgaaaatggt cagccggtcg agcttggcga ccacctctac cgggcaagct
       61 tgtactcgtt cgagatcgtt ctcgtcacgc gctaagtccc cggcaggagg gagggcctcc
      121 ttatggccag tatggaaggt caggtcgcgc tcgtcaccgg cggcgcccgc gggattgggc
      181 aggcgatcag cacccggctc gccgaccgcg gggtccgggt ggcggtcggc tactcccgcg
      241 gggcggacac ggccaaggcg ttcgcggccg cccatgaggg ggccaccatc caccagggca
      301 acatcggtgt cgccgccgac tgccaacggg tcatcaacga ggtcctggac cagcacgggc
      361 agctggacat cctggtcaac aacgccggca tcaccatcga caagacggtc cgcaagatga
      421 gtgtggagga ctgggaccgg gtcgtcggca tcaacctgtc gggggccttc tacctgagcc
      481 gggccgtgct ccagcacatg ctggaccggg gctatggccg gatcatcaac atcagctcgg
      541 tgatcgggga gaagggcaac atcggccagg ccaactacgc cgccgccaag tcgggcctgt
      601 tcgggctgac caacagcctg gccctggaga cggcgcgcaa ggggatcacg gtcaactcgg
      661 tcgcgcccgg gttcatcgcc accgagatgg tctcggccat gcccgaggac gtcctggcca
      721 aggtggtcgc ccagatcccg gttggtcggc ttggccagcc gcaggagatc gcccgggtgg
      781 tcgagttcct ggccgacccc gagtcggggt acatcaccgg ggagatcttc gacgtcaacg
      841 gcgggctgta catgtaggga accgagcaag gctaaggagt tagcacatgg ctgcagagcg
      901 tgaggtcgtc gtcctctccg gggtccgcac ggccgtcggg aagtacgggg ggagcctgaa
      961 ggacatgccg ccggccgacc tgggggcggc ggtggtccgc gaggcggtga gccggtccgg
     1021 ggccgagccg gccgacgtcg ggcacgtggt gttcggcaac gtcatccaca ccgagacccg
     1081 cgacatgtac ctgtccaggg tggccgcggt gaacggcggc ctcccggtcg agacgccggc
     1141 cttcaccctc aaccggctgt gcgggtcggg gctgcaggcg atcgtctcgg ccgcccagct
     1201 ggtcctgctc ggcgacaccg aggtggccct ggccggcggg gccgagtcga tgagccgcgg
     1261 gctgtacggg gtgccggcga tgcgctgggg agcccgcatg ggcgagacca gcgcctcgga
     1321 catgatggtc ggggccctga ccgacccctt cgacgactgc cacatggggg tcacggccga
     1381 gaacgtggcc ggcaagtggg gcatctcccg cgaggaccag gacgcgctgg ccgtgcagtc
     1441 ccaccagcgg gcggccaagg ccaccgccga gggctacttc aaggaccaga tcctccccat
     1501 cgagctcaag agccgcaagg gcaccaccac cttcgacacc gacgagcaca tccgggccga
     1561 cgccacgata gagggcatgg ccaagctgcg cccggtgttc gacaagagcg ggtcggtgac
     1621 cgccggcaac gccgccggga tcaacgacgc cgccgccgcg gtggtcctgg ccgacgccgg
     1681 ctgggccgag cgcaagggcc tggcgccgat gggccggctg gtcgcctacg cccacgccgg
     1741 ggtcgagccc aagtacatgg gcatcggtcc ggtgccggcg gtccggatgg tgctggagaa
     1801 ggccaacctc aaggtcgatg acatcgacgt gttcgagctc aacgaggcct tcgcggccca
     1861 ggccctggcc gtggtccgcg acctcagcct ccccgaggac cgcaccaacc ccaacggctc
     1921 gggcgtctcc ctcggccacc ccatcggggc caccggctgc atcctcaccg tcaaggccct
     1981 gtacgagctg gagcgcaccg gcggccgccg cgccctggtc acgatgtgca tcggcggcgg
     2041 ccagggcatc gccgccatct tcgaacgggc cgcatgacgc cagccgcgtg cggtgagcgt
     2101 ggggcgtacc gcggtgagcg tgggggcgta tgcggtgagc gtgggggggg catgcgtgag
     2161 cgtggggcgc atgcggtgag cgtgggggct gtggacagcc ccggacgcct cgttcccccg
     2221 gtggggtagc ctcccccacc gggggacccg gcatcggcgg gccagggtga aggccgccgg
     2281 ccgcggtgaa ggccgccggg cctccggccg cccgcccgtc ggcaacggtc tcgctggtgg
     2341 gcggccccgg atgggaccgg ctggggcgct gcgcggcccc ggctgcggcc gctggttcgt
     2401 ccagcgccac cgggcggcct ggcgcggcta cttctcgtgg atgtagctgc cgggggcgtc
     2461 ggcgatgggg gggtgcttgg cgttgcccat gggcgggggc ttgcggcgct ggccgccgtg
     2521 ggccttggcc caggggaccc agtcgtccca ccaggagccg gcgtgctcgg tcgcccccgc
     2581 cagccacacg tcggggtcgg gcgggatgtc gtcgttggtc cagtggcgcg ccttggggct
     2641 gggcgggttg acgatcccgg cgatgtggcc cgaggagctg agcacgaacc gggtgtcgcc
     2701 cttgaccagc tgggtggtgc tgtacgacga ctgccacggg gtgatgtggt cgttggccgc
     2761 cccgaccagg tacaggtcct gggtcaccgc gtccaggtgc agccgcaccc cggccagctc
     2821 catgtcgccc cgagccagct ggttgtccag gtagcaggtg cgcaggtaga acgagtgcat
     2881 ggccgcgggc atgcgggtgg agtcggcgtt ccaggccagg atgtcgaa
//
LOCUS       DASSWR010000679         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_421246,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000679 DASSWR010000000
VERSION     DASSWR010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421246"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFO47_11420"
     CDS             <1..910
                     /locus_tag="VFO47_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8789344.1"
                     /translation="ATRREATAALDEHHAVTRASDEARVEARLRAEELGKRIVEEFAI
                     DPDEAMAEYLPGEARLAELPEPDPRRQPSTELRRVATALDRRLTLLGRVNPLALEEFK
                     ALEERYSFLSGQLQDLKESRRDLLKVVRAVDERVREVFGSAFEDVAREFERTFGLLFP
                     GGEGRLVLTDPDDLLASGIEVEARPPGKKVRRLSLLSGGERSLVALAFYFAIFRARPS
                     PFYVLDEVEAALDDVNLHRFLDLLDDAREHAQLLVVSHQRRTMEAADALYGVSMQAEG
                     VSKVVSRRLRSGTLDGNDEAAAVATR"
     gene            928..2091
                     /gene="ftsY"
                     /locus_tag="VFO47_11425"
     CDS             928..2091
                     /gene="ftsY"
                     /locus_tag="VFO47_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET8789345.1"
                     /translation="MQILLVVAATLVIVSLLAVVPLVAGRRRRQDADEVRQVDLDREP
                     GVGDDAGQPPRPGREVADVDLPPDDRGVATAAPPAEEVIAPRRRFRERLGRSRAALSR
                     GMAGLLARGADDDEAWEELEEALLSADVGVATSTELVERLRTRARAERASGSELRELL
                     RAELRDAVGSGDRSLALDLLEEHQRPGIVLVVGVNGTGKTTTVGKLALALAEEGKQPV
                     LAAADTFRAAATEQLVIWGDRVGAPVVRHQQGADPAAVAFDGLASARARGADVLLVDT
                     AGRLHTKTNLMEELRKLRRVLEREGGQVREVLLVLDATTGQNGLAQARQFTEAVGVTG
                     VVLTKLDGTAKGGIVIAVQRELGVPVKLVGLGEGPDDLAPFEPEAFVDALLAE"
BASE COUNT          266 a          839 c          748 g          250 t
ORIGIN      
        1 cgccacccgc cgcgaggcca ccgccgccct cgacgagcac cacgccgtca cccgggcctc
       61 cgacgaggcc agggtcgagg cccgcctgcg cgccgaggag ctgggcaagc gcatcgtcga
      121 ggagttcgcc atcgaccccg acgaggccat ggccgagtac ctgcccggcg aggcccgcct
      181 ggccgagctg cccgagcccg acccgcgccg ccagccctcg accgagctgc gccgggtcgc
      241 caccgccctc gaccgccgcc tcaccctgct gggccgggtc aaccccctgg cccttgagga
      301 gttcaaggcc ctggaggagc gctacagctt cctctccggc cagctccagg acctcaagga
      361 gagccgccgc gacctgctca aggtggtcag ggcggtcgac gagcgggtcc gggaggtgtt
      421 cggctcggcc ttcgaggacg tggcccgcga gttcgagcgc accttcggcc tgctgttccc
      481 cggcggcgag ggccgcctcg tcctcaccga ccccgacgac ctgctggcca gcgggatcga
      541 ggtcgaggcc cggccccccg gcaagaaggt ccgccgcctg tcgctgctgt cgggcggcga
      601 gcgcagcctg gtcgccctgg ccttctactt cgccatcttc cgggcccgcc ccagcccctt
      661 ctacgtcctg gacgaggtcg aggccgccct cgacgacgtc aacctgcacc gcttcctcga
      721 cctgctcgac gacgcccgcg agcacgccca gctgctggtc gtcagccacc agcgccggac
      781 catggaggcg gccgacgccc tctacggcgt ttccatgcag gccgagggtg tcagcaaggt
      841 cgtgagccgg cggctccgct ctggcaccct cgacgggaac gacgaggccg ccgccgtggc
      901 aacgaggtga ggtagggagt ggaagggctg cagatcctgc tcgtggtcgc cgccaccctg
      961 gtgatcgtga gcctgctggc cgtggtgccg ctggtcgccg ggcgccgccg gcgccaggac
     1021 gccgacgagg tccgccaggt cgacctggac cgtgagccgg gcgtcggcga cgacgccggg
     1081 cagccgccac ggccgggccg ggaggtcgcc gacgtcgacc tcccccccga cgaccgcggc
     1141 gtcgccaccg cggctccgcc ggccgaggag gtcatcgccc cccggcggcg cttccgcgag
     1201 cgcctcggcc gctcccgggc cgccctctcc cgcggcatgg ccgggctgct ggccaggggc
     1261 gccgacgacg acgaggcctg ggaggagctg gaggaggcgc tgctctcggc cgacgtcggc
     1321 gtggccacct ccaccgagct ggtcgagcgg ctgcggaccc gggcccgggc cgagcgggcc
     1381 agcggatccg agctgcgcga gctgctccgg gccgagctcc gcgacgccgt cgggtccggc
     1441 gaccggtcgc tcgccctcga cctgctcgag gagcaccagc gccccgggat cgtgctggtc
     1501 gtcggcgtca acggcaccgg caagacgacc accgtcggca agctcgccct ggccctggcc
     1561 gaggagggca agcagccggt gctggccgcc gccgacacct tccgggccgc cgccaccgag
     1621 cagctcgtca tctggggcga ccgggtcggc gccccggtcg tccgccacca gcagggcgcc
     1681 gacccggccg cggtcgcctt cgacggcctc gcctcggccc gggcccgggg cgccgacgtc
     1741 ctcctggtcg acaccgccgg ccgcctccac accaagacca acctgatgga ggagctgcgc
     1801 aagctccgcc gggtcctgga gcgcgagggc ggccaggtcc gcgaggtcct gctggtcctg
     1861 gacgccacca ccggccagaa cggcctcgcc caggcccgcc agttcaccga ggccgtcggc
     1921 gtcaccggcg tggtcctgac caagctcgac ggcaccgcaa agggcggcat cgtcatcgcc
     1981 gtccagcgcg agctcggggt cccggtcaag ctcgtcggcc tcggggaggg cccggacgac
     2041 ctggcaccgt tcgagcccga ggccttcgtc gacgccctcc tcgcggagta gcgcgccccg
     2101 gaa
//
LOCUS       DASSWR010000680         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1127227, whole genome shotgun sequence.
ACCESSION   DASSWR010000680 DASSWR010000000
VERSION     DASSWR010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127227"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..805)
                     /gene="rnc"
                     /locus_tag="VFO47_11430"
     CDS             complement(20..805)
                     /gene="rnc"
                     /locus_tag="VFO47_11430"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017605404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET8789346.1"
                     /translation="MEKALGVRFGGEELYVWAFTHRSFAYENGGLPTNERLEFLGDAV
                     LGLVVTDIIYRAFPDLPEGQLAKLRAATVNMNVLAEVARELGVGDAVRLGRGEELSGG
                     RDKSSILADTLEAVLGAIYLDKGLSRAAALVRRLFEHRVMEAAGRGAALDYKTSLQEL
                     AASSLGGMPSYAIEEEGPDHAKRFTATVSVAGTAYGSGKGRSKKEAEQQAAREAFESL
                     AADRMVSGTGDAGQVDVVPVDRGPRLEAPGTPAESPAPPPAGE"
     gene            complement(816..992)
                     /gene="rpmF"
                     /locus_tag="VFO47_11435"
     CDS             complement(816..992)
                     /gene="rpmF"
                     /locus_tag="VFO47_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET8789347.1"
                     /translation="MPVPKRKTPRAKTRHRRSHWKSVAPTYASCPQCRQAKLPHTVCQ
                     NCGYYGGRQVVEVE"
     gene            complement(973..1536)
                     /locus_tag="VFO47_11440"
     CDS             complement(973..1536)
                     /locus_tag="VFO47_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET8789348.1"
                     /translation="MSKHVTAPALAVDVRELLQKPGAHRHVVVGAPLDDLATPVASVP
                     PDRPVTVDAEVESVVEGLLVTGKVSATAVVRCVRCLRDLDHEVEVEVRELFALEPGED
                     EDEGYAVLPDDRLPLDTMARDALVLAFPAFPLCRPDCAGLCPVCGADRNSTDCGHGGA
                     DDIDPRWAGLADLRTSIQEEDDARPQA"
     gene            complement(1652..>2080)
                     /gene="coaD"
                     /locus_tag="VFO47_11445"
     CDS             complement(1652..>2080)
                     /gene="coaD"
                     /locus_tag="VFO47_11445"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET8789349.1"
                     /translation="IVARAARCFDRVIVAVLINPAKQPMFTLEERLAMLKEATEPMGS
                     VEIDAFSGLLVDYARRREVTAIVKGLRAVSDFDYELQMAQMNYRLAGVDTMFMTTNPT
                     WSYLSSSLVKDVARHGGDVTGLVPDFVVARLRERAQQPPA"
BASE COUNT          292 a          709 c          784 g          295 t
ORIGIN      
        1 agctcgggaa gctcgggcac tactcgcccg ctggcggggg cgcaggagat tcggcggggg
       61 ttccgggggc ctcaagccta ggcccccggt caacggggac cacgtcgacc tggccggcgt
      121 cgccggtgcc gctgaccatg cggtcggcgg ccagggactc gaacgcctca cgggccgcct
      181 gctgctcggc ctccttcttg gagcggccct tgccgctgcc gtaggccgtg ccggcgaccg
      241 agacggtggc cgtgaaccgc ttggcgtggt cggggccctc ctcctcgatg gcgtaggagg
      301 gcatcccgcc gaggctggac gcggccagct cctgcaggga ggtcttgtag tcgagggcgg
      361 ccccgcgccc ggccgcctcc atcacccggt gctcgaacag ccgccgcacc agggcggccg
      421 cccgcgacag gcccttgtcg aggtagatgg ccccgagcac ggcctcgagg gtgtcggcca
      481 ggatggagga cttgtcccgc ccgcccgaga gctcctcgcc ccggcccaga cggacggcgt
      541 cgccgacgcc gagctcgcgg gccacctcgg ccagcacgtt catgttcacc gtggccgccc
      601 gcagcttggc cagctggccc tcgggcaggt cggggaaggc gcggtagatg atgtcggtga
      661 cgaccaggcc gaggacggcg tcgccgagga actcgagccg ctcgttggtg ggcaggccgc
      721 cgttctcgta ggcgaaggag cggtgggtga aggcccagac gtagagctcc tcgccgccga
      781 agcggacgcc gagggccttc tcgatcggct gtcgcttact cgacctcaac gacctgacgc
      841 ccaccgtagt acccgcagtt ctggcagacg gtgtgcggca gcttggcctg ccggcactgg
      901 gggcacgacg cgtaggtcgg ggccaccgac ttccaatggg accggcggtg ccgggtcttg
      961 gcccgcgggg tcttacgctt ggggacgggc atcgtcttcc tcctggatgc ttgtgcgcag
     1021 gtcggccagg ccggcccagc ggggatcgat gtcgtcggcc ccgccgtgcc cgcagtcggt
     1081 gctgttgcgg tcggcgccgc agacggggca gaggccggcg cagtcggggc ggcacagcgg
     1141 gaaggccggg aaggcgagca cgagcgcgtc cctggccatg gtgtcgaggg gcaggcggtc
     1201 gtcgggcagc acggcgtagc cctcgtcctc gtcctcgccg ggctcgaggg cgaacagctc
     1261 gcgcacctcg acctccacct catggtcaag gtcgcgcagg cagcggacgc agcggaccac
     1321 cgcggtggcg ctgaccttgc cggtgaccag cagcccctcg accacgctct cgacctcggc
     1381 gtcgacggtg acgggccggt cgggcgggac cgaggcgacc ggggtcgcca ggtcgtcgag
     1441 gggggcgccc accaccacgt ggcggtgcgc cccgggcttc tggagcagct cgcggacgtc
     1501 gaccgcgagc gccggtgctg tgacgtgctt gctcatgaac atgacaatcc gaggtgatgg
     1561 cgcctggggc gtgtgagggg cacacaaaaa cgacggtggg aacgcaccgc agcgacccag
     1621 ggtagcagaa agccggacgg gcggagcgcg gtcaggcggg tggctgctgg gcgcgttcgc
     1681 gcagcctggc caccacgaag tcgggcacca gcccggtgac gtcgccgccg tgcctggcca
     1741 cgtccttgac caggctggag ctgaggtacg accaggtcgg gttggtggtc atgaacatgg
     1801 tgtcgacccc ggccaggcgg tagttcatct gcgccatctg gagctcgtag tcgaagtccg
     1861 agaccgcccg caggcccttg acgatggcgg tgacctcgcg ccgcctggcg tagtcgacca
     1921 gcaggccgga gaaggcgtcg atctcgaccg agcccatcgg ctcggtcgcc tccttgagca
     1981 tggccagccg ctcctcgagg gtgaacatcg gctgcttggc cgggttgatg agcacggcca
     2041 cgatcacccg gtcgaagcac ctggccgccc gggccacgat
//
LOCUS       DASSWR010000681         4018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_986231,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000681 DASSWR010000000
VERSION     DASSWR010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986231"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..2036
                     /gene="nuoL"
                     /locus_tag="VFO47_11450"
     CDS             138..2036
                     /gene="nuoL"
                     /locus_tag="VFO47_11450"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET8789350.1"
                     /translation="MLFGQRLRALAGWIATLAVAAAFAVAVAACFELLSHPGEERTQI
                     LRGYEWFVSGDLRVAFDLRWDPLSAVMALVVTGVSSLIHLYSIGYMAHDERRETFFSW
                     LNLFVASMLVLVLAQNFLVMFLGWEGVGLCSYLLVGFWFNRRLLVVNPRGRLVEGEPD
                     DTREVWAPPAAKKAFLANRIGDVGFLLAMFLIFATTGSLDFDVVFGEAGALAAGTATA
                     IALLLFLACTGKSAQFPLYVWLPDAMAGPTPVSALIHAATMVTAGVFLVARTHSIFEA
                     SGTAQTVVLLVGAGTALLAALIAIGQDDIKKILAYSTVSQLGYMFIGVALGPIGYAAG
                     MLHLVTHAFFKAQLFLGAGSVMHGNDDDTDIKHFGGLRSAMPITFWTTMAAWAAIVGV
                     PFTSGFYSKDQVLAALFEHGGLGLLAWAIGVFTAGLTAFYMSRYVFLTFFGERRWPEG
                     RHPHESPPVMTIPLIVLGVLALVAGFALTTFTGEGGRFQQFLEPVLGAPEEPESLAAA
                     AGLSAVATGLAAVGAVVAWLLYIRPFDWLALRRRWAGAWRPLAERLYVDQVYEFFTVN
                     LGGAFAAFLARNVDQRGIDGAVNGVAELVGDAARSGRRLQSGLVRSYALGVLGGAVLI
                     VAFLVFRP"
     gene            2062..3540
                     /locus_tag="VFO47_11455"
     CDS             2062..3540
                     /locus_tag="VFO47_11455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET8789351.1"
                     /translation="MLLTATLFAPLVGALLVALVPAARQETARLVALAASLATLALAV
                     VLAARFRTGDPGFQLGMTAEWVPAFGVSYRVGVDGVSLPLVLLTAVLTPLAIAGSWRM
                     ADRPRTYLGLLLVLETAMLGVFVSLDLFLFYVFWEAVLVPGYFLIGSFGGERRVYAAV
                     KFFVYTLLGGLLMLVGIIALFWLHREATGTPTFDYQALVDFDKSPATQRWLFAAFFAA
                     FAIKTPVWPFHTWLPDAYTEAPTTTTVLLAGVMSKLGVYGFLRFALPLFPDGARWAQP
                     LLLVLAVVGILYCALVATMQRDFKTLVAYSSVSHLGFIVLGIFAFNLQGLQGGVFYMV
                     AHGLVIGGLFFVTGMLEERRGTRRIDEFGGLQQAVPRMAGVMLVITLGSVALPGLVGF
                     VGEFLTLLGAFLENRVYAVLATVGVILGAVYLLWAYQRMWHGPLEREENRAMPDLNGR
                     EWAILAPLVAAIIVLGLFPRPVLERVEPSAQRVVEQTQAPSP"
     gene            3554..>4018
                     /locus_tag="VFO47_11460"
     CDS             3554..>4018
                     /locus_tag="VFO47_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789352.1"
                     /translation="MHPLAQANTQPLPVPEVDWRAIAPELALLVAGVVLLLVVAFWPK
                     AGRGVLAGLTLAGLAVAAALTAWNWDLERVAFEGAVSLDGITRYARVILLAVGVLATL
                     MVAQGDTREGEPDDSLPEVYPLLLFALLGMVLLAAASDLLVVFIALEILSLSL"
BASE COUNT          420 a         1553 c         1325 g          720 t
ORIGIN      
        1 catcttccgc aagcgctcgt cgatctcggt cgacgacatc aacctgctca aggggtgacg
       61 gtgccctgaa cgcgaccggc gcgacccgcc tgatctggct gctcccgatc ctgcccctgg
      121 ccggcttcgc ggtcaacgtg ctgttcggcc agcgcctgcg cgccctggcc ggctggatcg
      181 ccaccctggc cgtcgccgcc gccttcgccg tcgccgtggc cgcctgcttc gagctgctgt
      241 cgcaccccgg cgaggagcgc acccagatcc tgcggggcta cgagtggttc gtctccggcg
      301 acctgcgggt cgccttcgac ctccgctggg atcccctgtc ggccgtcatg gccctggtcg
      361 tgaccggcgt ctccagcctc atccacctgt actcgatcgg ctacatggcc catgacgagc
      421 ggcgcgagac cttcttctcc tggctcaacc tgttcgtggc ctcgatgctg gtgctcgtcc
      481 tggcccagaa cttcctggtc atgttcctcg gctgggaggg ggtggggctc tgctcctacc
      541 tgctggtcgg gttctggttc aaccggcggc tgctggtcgt caacccgcgg ggccgcctgg
      601 tcgaaggcga gcccgacgac acccgcgagg tgtgggcgcc gcccgcggcc aagaaggcct
      661 tcctggccaa ccgcatcggc gacgtcggct tcctgctggc catgttcctg atcttcgcca
      721 ccaccggcag cctcgacttc gacgtcgtct tcggcgaggc cggcgccctg gccgcgggca
      781 cggccacggc catcgccctg ctgctgttcc tggcctgcac gggcaagagc gcccagttcc
      841 cgctgtacgt ctggctgccc gacgccatgg ccggcccgac cccggtgtcg gccctcatcc
      901 acgccgccac catggtcacc gccggggtct tcctggtcgc ccggacccac tccatcttcg
      961 aggcctcggg caccgcccag acggtggtgc tgctggtcgg cgccggcacc gccctgctgg
     1021 ccgcgctcat cgccatcggc caggacgaca tcaagaagat cctcgcctac tcgaccgtct
     1081 cccagctcgg ctacatgttc atcggcgtcg ccctggggcc gatcggctac gccgccggca
     1141 tgctccacct ggtcacccac gccttcttca aggcccagct gttcctcggg gccgggtcgg
     1201 tgatgcacgg caacgacgac gacaccgaca tcaagcactt cggcgggctg cgctcggcca
     1261 tgccgatcac cttctggacg accatggccg cctgggcggc catcgtcggg gtgccgttca
     1321 cctccggctt ctactccaag gaccaggtgc tggccgccct gttcgagcac ggcgggctcg
     1381 gcctgctggc ctgggcgatc ggggtgttca cggccggcct gaccgccttc tacatgtccc
     1441 gctacgtgtt cctgaccttc ttcggcgagc ggcgctggcc cgagggccgc cacccgcacg
     1501 agtcgccgcc ggtcatgacc atcccgctga tcgtgctcgg cgtcctggcc ctggtggccg
     1561 ggttcgccct caccaccttc accggcgagg gcggccgctt ccagcagttc ctggagccgg
     1621 tcctcggcgc gcccgaggag cccgagtcgc tggccgccgc ggccggtctg tcagcggtgg
     1681 ccaccgggct ggccgcggtc ggtgccgtcg tcgcctggct gctgtacatc cggccgttcg
     1741 actggctggc cctgcgccgc cgctgggccg gcgcctggcg gcccctggcc gagcggctct
     1801 acgtcgacca ggtctacgag ttcttcaccg tcaacctggg cggcgccttc gccgccttcc
     1861 tggcccgcaa cgtcgaccag cgtggcatcg acggggccgt caacggcgtg gccgagctgg
     1921 tcggggacgc cgcccgcagc ggccgccgcc tccagtccgg cctggtccgc agctacgccc
     1981 tcggcgtcct cgggggcgcc gtgctgatcg tcgccttcct ggtgttccgg ccatgacccc
     2041 ctcgtccctg gagccgccgc cgtgctgctg accgcaaccc tgttcgcccc cctggtcggg
     2101 gcgctgctgg tcgccctcgt cccggcggcc cggcaggaga cggcccggct ggtggcgctg
     2161 gcggccagcc tggccacgct cgccctggcg gtcgtgctgg cggcccgctt ccgcaccggc
     2221 gaccccgggt tccagctcgg catgaccgcc gagtgggtgc cggccttcgg ggtcagctac
     2281 cgggtcgggg tcgacggggt cagcctgccc ctggtcctgc tcaccgccgt gctcaccccg
     2341 ctggccatcg ccggctcctg gcgcatggcc gaccggccgc gcacctacct gggcctgctg
     2401 ctggtgctgg agacggccat gctgggcgtg ttcgtgagcc tggacctgtt cctgttctac
     2461 gtgttctggg aggcggtgct ggtcccgggc tacttcctga tcggctcgtt cgggggcgag
     2521 cggcgggtgt acgccgcggt caagttcttc gtctacaccc tgctcggcgg gctgctcatg
     2581 ctggtcggca tcatcgccct gttctggctg caccgggagg cgaccggcac cccgaccttc
     2641 gactaccagg ccctggtcga cttcgacaag tcgccggcca cccaacgctg gctgttcgcc
     2701 gccttcttcg ccgccttcgc catcaaaacc ccggtgtggc cgttccacac ctggctgccc
     2761 gacgcctaca ccgaggcccc gaccaccacc accgtgctgc tggccggggt catgtccaag
     2821 ctgggcgtgt acgggttcct ccgcttcgcc ctgccgctgt tccccgacgg cgcccgctgg
     2881 gcccagccgc tgctgctcgt cctggccgtg gtcgggatcc tctactgcgc cctggtcgcc
     2941 accatgcagc gcgacttcaa gaccctggtc gcctactcgt ccgtgtccca cctgggcttc
     3001 atcgtgctcg gcatcttcgc cttcaacctc caggggctcc agggcggggt gttctacatg
     3061 gtcgcccacg ggctggtcat cggcgggctg ttcttcgtca ccggcatgct cgaggagcgc
     3121 cgcggcaccc gccggatcga cgagttcggc ggcctccagc aggccgtgcc ccgcatggca
     3181 ggggtgatgc tggtgatcac cctcggctcg gtcgccctgc ccggcctggt cgggttcgtg
     3241 ggcgagttcc tcaccctgct cggggccttc ctggagaatc gcgtctacgc cgtgctggcc
     3301 accgtcgggg tgatcctggg cgccgtctac ctgctctggg cctaccagcg gatgtggcac
     3361 gggccgctgg agcgggagga gaaccgggcc atgcccgacc ttaacggccg ggaatgggcc
     3421 atcctggccc cgctggtcgc cgccatcatc gtcctcggcc tgttcccccg gccggtgctc
     3481 gaacgggtcg agccgtcggc ccagcgcgtc gtcgagcaga cccaggcccc gagtccctga
     3541 ccccggagga gccgtgcacc ccctggccca agccaacacc cagcccctgc ccgtgccgga
     3601 ggtcgactgg cgcgccatcg cccccgagct ggccctgctc gtcgccgggg tggtgctgct
     3661 gctggtggtg gccttctggc cgaaggccgg gcgcggcgtc ctggccggcc tgaccctggc
     3721 cgggctggcc gtcgcggccg ccctcaccgc ctggaactgg gacctggagc gggtcgcctt
     3781 cgagggcgcc gtctccctgg acggcatcac ccgctacgcc agggtcatcc tgctcgccgt
     3841 cggcgtcctg gccaccctga tggtcgccca gggcgacacc cgcgagggcg agcccgacga
     3901 cagcctgccc gaggtctacc cgctgctgct gttcgccctg ctcgggatgg tgctcctggc
     3961 cgccgcctcg gacctgctgg tggtgttcat cgccctggag atcctctcgc tcagcctg
//
LOCUS       DASSWR010000682         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_619072,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000682 DASSWR010000000
VERSION     DASSWR010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619072"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..574
                     /locus_tag="VFO47_11465"
     CDS             116..574
                     /locus_tag="VFO47_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8789353.1"
                     /translation="MRITVVGPADLPELLPLMRTYCDFYQAAPADQALLELSQALLAD
                     PQREGLQLLARDDTGGAVGFATLFWTWQTLAAARVGVMNDLYVAAEARGTGMADGLIA
                     ACLERCREHGATRLVWQTAADNHRAQAVYQRLGATRDERWLDYQLPVDPG"
     gene            943..1251
                     /locus_tag="VFO47_11470"
     CDS             943..1251
                     /locus_tag="VFO47_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789354.1"
                     /translation="MDTEVGRTAGTATTDRRGLARRVSTETKAAYKTTEFITYVVVFA
                     GILVASFLVKTGQDGQRIDYFRADKAWWYITLLTIGYMIARGLAKSGSREPYDNSDRR
                     "
     gene            1359..2000
                     /locus_tag="VFO47_11475"
     CDS             1359..2000
                     /locus_tag="VFO47_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789355.1"
                     /translation="MTDSTFGSTATADRSTTEQVNDQVRGTTGLAQDKARGALGQARS
                     RLRDQIDQRSTQAGEQVQSTAQDMRTMAEQLRGQGKDTPARVAEQVADRAETFGSYLR
                     DADGERLLRDVEDVARRQPWLVAAGGVALGFAASRFLKASSSRRYQTGDDRSSAGHSV
                     DDVTWATGAPSTSTPADNGSEIPFTQADVVAPAVGSNPSATQPITPVTESRRR"
     gene            2013..2627
                     /locus_tag="VFO47_11480"
     CDS             2013..2627
                     /locus_tag="VFO47_11480"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET8789356.1"
                     /translation="MGQDPDAIRDEIAQTRADMSEAVEAIGYKADVPTRAKDKVSQKV
                     EDVKSKVSETATRAKEAVTGTTSRVGDAASGTAARVGEATPSGGEAKQQAKRAVGLAK
                     ENPLGLAVGAVALGFLAGLVVPSTRVENERLGPVADQVKDKVKETGQEALDRGKQVAQ
                     EAASSAADTARRQGREHGQDLAQSVKQNAQDVRDQATQSSTTRS"
     gene            2734..>3000
                     /locus_tag="VFO47_11485"
     CDS             2734..>3000
                     /locus_tag="VFO47_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789357.1"
                     /translation="MPTKTLRGNDPQTPILSAVRESQTAFIDVVRSWTDITEQLTRRL
                     SLPVAGIDVAGALDRTFDLAEQTLAAQRQFALTLIGAVDRQVDTV"
BASE COUNT          511 a         1056 c         1031 g          402 t
ORIGIN      
        1 ttccgcgggg aagaaccccg ctgctgcagc tgaaggttgg aatgacaggg tgtgcgcttc
       61 gaccgggcac cgagccgttc ctgcccgccg gtttgggcgt gaggagggag tgcccgtgcg
      121 gatcaccgtg gtcggccccg ccgacctgcc ggagctgctg ccgctgatgc gcacctactg
      181 tgacttctac caggctgcac ccgccgacca ggctctgctc gagctatcgc aagcgctgct
      241 cgccgacccg caacgggagg ggctgcagct gctggcccgc gacgacaccg gcggggctgt
      301 cggcttcgcc accctcttct ggacctggca gacgctggcc gccgcccgcg tcggggtgat
      361 gaacgacctg tacgtggccg ccgaggcgcg cggcaccggg atggccgacg gactcatcgc
      421 cgcctgcctg gagcgctgcc gcgagcacgg cgcaacccgc cttgtctggc agacggcggc
      481 cgacaaccac cgcgcgcagg ccgtctatca gcggcttggg gcgacccgcg atgagcgctg
      541 gctggactac cagctgccgg ttgatcctgg ctgacttctg gcggcgcagg gacggcccgc
      601 cgaggtgcgg agggccagtc aggaacggtg cctggctggg ccgtcctgtg gccggagggt
      661 cggattccgg cctgagggtt taggcggagt ctcggtggct accctcccga atgtcgggga
      721 ggtgctccgg ctgtcggtgg gaccaggtcc tcgtcccaca cgtgcggagg ttgccaggca
      781 gccggtccgt cccgttcccc gcttcacagc gtgctcgagc gcgctcccga atccaggcga
      841 ggcgttgcca gcctgccggt gagtgtgctc tgtggcacgg cgcacgccca ccatcaccca
      901 gcttgggtca ttcaacccgg gcgatcactg aaaggcagca gcatggacac agaggttggc
      961 cgcactgccg gcacagccac caccgatcgg cgcggcctcg cccgccgggt cagcaccgag
     1021 accaaggccg cctacaagac caccgagttc atcacctatg tggtggtgtt cgccggcatc
     1081 ctggtggcct cgttcctggt caagaccggt caggacggcc agcggatcga ctacttccgg
     1141 gccgacaagg cctggtggta catcaccctg ctcaccattg gctacatgat cgcccggggg
     1201 ctggccaagt cgggtagccg cgagccctac gacaacagcg accgccgcta accgcggctg
     1261 ggctgggccg actctcggcc cggccgtatc gctcagcggc cgccggacgc gctctccgcg
     1321 agctgtccgg ctgccgcggt agagaaagga agcaccgcat gaccgactcc acattcgggt
     1381 cgaccgcgac cgctgaccgg tcgaccaccg agcaggtcaa cgaccaggtc cgcggcacga
     1441 cagggctggc ccaggacaag gccaggggag cgctcggcca ggcccgcagc cggctccgtg
     1501 accagatcga ccaacgttcc acccaggccg gcgagcaggt ccagtcgacc gcgcaggaca
     1561 tgcgcaccat ggctgagcag ctgcggggcc agggcaagga cacgccagcc cgggtggccg
     1621 agcaggttgc cgaccgggcc gagaccttcg gcagctacct gcgcgacgcc gatggcgagc
     1681 ggctgctccg ggatgtggag gacgttgccc gccgtcagcc ctggcttgtc gcggctggcg
     1741 gggtggcgct gggctttgcc gcctcccggt tcctgaaggc ctccagcagc cgtcgctacc
     1801 agaccgggga cgaccgttcg tcggccggcc acagcgtgga cgacgtgacc tgggccaccg
     1861 gagccccgtc cacctccacc ccggccgaca acggctccga gatcccgttt acccaagccg
     1921 acgtggtcgc gccggccgta ggcagcaacc cctcggccac ccagcccatc acgccagtca
     1981 ccgagtccag gagacggtaa ggaggacgtg gcatgggcca agacccagat gcgatccgag
     2041 acgagatagc gcagacacgg gccgatatga gcgaggcggt ggaggcgatc ggctacaagg
     2101 cggacgtgcc cacccgagcc aaggacaagg tgagccagaa ggtggaagac gtgaagtcca
     2161 aggtgagtga gaccgccacc cgggccaagg aggccgtgac cggcacaacc tcccgggtcg
     2221 gggacgcggc aagcgggacc gcagcccggg tgggcgaggc caccccgtcc ggcggtgagg
     2281 ccaagcagca ggccaagcga gcggtcggcc tggccaagga gaacccgctc gggctggccg
     2341 tcggtgcggt cgcgctgggc ttcctggccg gccttgttgt ccccagtact cgggtggaga
     2401 acgagcggct ggggccggtg gccgatcagg tcaaggacaa ggtcaaggag accggccagg
     2461 aggccctgga ccgcggcaag caggtcgccc aggaggcagc cagcagcgcc gccgacaccg
     2521 ccaggcggca gggacgtgag cacggccagg atctcgccca gagcgtcaag cagaacgccc
     2581 aggacgtccg tgaccaggca acgcaatcct ctaccaccag gtcatagacg ctgctcgccg
     2641 tacctgctcg actgcattgc ttgttaaagt aagcgcctgc caactaaact atgcataacc
     2701 ggtatccgct ggtcatgacg agaggaacca cccgtgccga ccaagacctt gcggggcaac
     2761 gacccgcaga cccccatcct gagcgccgta cgcgagagcc agacggcgtt catcgacgtg
     2821 gtccgcagct ggaccgacat caccgagcag ctgacccgac gcctgtccct gccggtggcc
     2881 ggcatcgacg tcgccggcgc cctggaccgc accttcgacc tcgccgagca gaccctcgcc
     2941 gcccagcgcc agttcgccct caccctcatc ggtgcggtcg accgccaggt cgacaccgtc
//
LOCUS       DASSWR010000683         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_534718,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000683 DASSWR010000000
VERSION     DASSWR010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534718"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1212)
                     /locus_tag="VFO47_11490"
     CDS             complement(130..1212)
                     /locus_tag="VFO47_11490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8789358.1"
                     /translation="MTAEGQPGAAETLARRLFADALGALELYTVYVGERLGLYRALAE
                     GGPATSAELAGRTGTVERYVREWLEHHAASGLLAVDDPAADPTARRFHLPAGHVPVLA
                     DPDDVRYAAFKGVELVRAARPLPDVVEAFRHGGAPPPLSWAPEGRAEFNRALFVNLLG
                     REWLPAIGEVDRRLRAEPPARVADLGCGTGWSSIAMATAYPRIRVDGLDLDQDVIAAA
                     NENAREAGLTDRVRFSVLDAADPDLPGRYDLVTIFEALHDMARPVEALAAARGMLAEG
                     GSVLVADEPVADQLTVPASEMERYAYGWSVVSCLPSAMGDPGTAATGAVMRPATLARY
                     AQVAGFAHTEALPIDTDVWRFYRLVP"
     gene            complement(1250..2881)
                     /locus_tag="VFO47_11495"
     CDS             complement(1250..2881)
                     /locus_tag="VFO47_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET8789359.1"
                     /translation="MMDHPLTVRTIYERSRTLFPDKPLVTRRPRGVRRTTYGDWAERV
                     ARLAGGLRELGVGPGDRVATFCWNHDRHLEVYFAAALMGASYHTLNIRLAGDQLAYIV
                     SHARDKVLVCDAELAQAIGPVLGELDTVEHVLVLDAEDGERPSLGFAGERGYEDLVER
                     SAPMTDWPALDEGQTAGICYTSATTGNPKGVAYSHRALYLHSLVVGLADTAAISERDV
                     LLPIVPMFHVNAWGMPFAATWYGAGQVYGGPAPTPADYLRLIREEGVTVSAGVPTVWL
                     GILQLLEKDGGDLGPLQRIICGGSAAPVSMIRAYEERHGVQFIHAYGMTEATPVTHFS
                     RLKSTMDDLPVEARYGYKAKQGLLVPGLEQRVVDADGHEVPADGKSMGELQLRGPTIA
                     SEYLDDPRSAQTFVDGWYHTGDVATVDHDGYLHLVDRTKDLVKSGGEWISSVELENAL
                     MGHPEVAQAAVIAVPDEKWGERPLAVVVRKPGAEVGEAEVVASVAGKFPKWWLPERVE
                     FVDEIPMTATGKFSKRLLRERFVASVQEEDASDDR"
BASE COUNT          384 a         1085 c         1080 g          379 t
ORIGIN      
        1 caccccgagc tgcggaccag caaggggacc atccgcctgc gtcccgccga cgccgccggc
       61 atccccgacg acgagttccg cgacctggcc cgggctgccc tcgacgcctg accgcggggc
      121 acgcggcggc tagggaacca gccggtagaa ccgccagacg tcggtgtcga tcgggagcgc
      181 ctcggtgtgg gcgaaaccgg ccacctgggc gtagcgggcc agggtggccg ggcgcatgac
      241 ggctccggtg gcggccgtcc ctgggtcgcc catggcgctg ggcaggcacg acaccacgct
      301 ccagccgtag gcgtagcgct ccatctcgga ggcggggacg gtgagctggt cggcgacggg
      361 ctcgtcggcc accagcaccg agccgccctc ggccagcatc cccctggccg cggccagcgc
      421 ctcgaccggg cgggccatgt cgtgcagggc ctcgaagatc gtcaccaggt cgtagcggcc
      481 cggcaggtcc gggtcggcgg cgtcgagcac cgagaaccgc acccggtcgg tcagcccggc
      541 ctcgcgcgcg ttctcgttcg cggcggcgat cacgtcctga tcgaggtcga gcccgtccac
      601 cctgatccgc gggtaggcgg tggccatggc gatgctcgac cagccggtgc cgcagcccag
      661 gtcggccacc cgggccggcg gctcggcccg cagccgccgg tcgacctccc cgatggccgg
      721 cagccactcg cggccgagca ggttgacgaa cagcgcccgg ttgaactcgg cccggccctc
      781 cggcgcccag gagagcggcg ggggcgcgcc cccgtgccgg aacgcctcca ccacgtcggg
      841 cagcggccgg gcggcgcgga ccagctcgac ccccttgaag gccgcgtagc ggacgtcgtc
      901 ggggtcggcc aggaccggga cgtgcccggc cgggaggtgg aagcgccggg ccgtcgggtc
      961 ggcggcgggg tcgtcgacgg ccagcaggcc gctggccgcg tggtgctcca gccactcccg
     1021 gacgtagcgc tcgacggtcc cggtgcgccc ggccagctcc gcggaggtcg ccggcccgcc
     1081 ctcggccagg gcccggtaca ggccgagccg ctccccgacg tagacggtgt acagctccag
     1141 cgcccccagg gcgtcggcga acagccgccg ggccagggtc tcggccgcgc cgggctggcc
     1201 ctcagcggtc atcgtgtcag ggtcgtcctc ggacggctga tccgcggcct cagcggtcat
     1261 cggaggcgtc ctcctcctgg acgctggcca cgaagcgctc gcgcagcagg cgcttggaga
     1321 acttgccggt ggcggtcatg gggatctcgt cgacgaactc gacccgctcg ggcagccacc
     1381 acttggggaa cttgccggcg accgaggcga ccacctcggc ctcgccgacc tcggcgccgg
     1441 gcttgcggac gaccacggcc aggggccgct cgccccactt ctcgtcgggg acggcgatga
     1501 ccgccgcctg ggcgacctcg gggtggccca tcagggcgtt ctccagctcg acgctggaga
     1561 tccactcgcc gccggacttg accaggtcct tggtccggtc gaccaggtgc aggtagccgt
     1621 cgtggtcgac agtggccacg tccccggtgt ggtaccagcc gtccacgaag gtctgggccg
     1681 agcgcgggtc gtccaggtac tccgaggcga tggtggggcc gcgcagctgg agctcgccca
     1741 tcgacttgcc gtcggccggc acctcgtggc cgtcggcgtc caccacccgc tgctccaggc
     1801 ccgggacgag caggccctgc ttggccttgt agccgtagcg ggcctccacc ggaaggtcgt
     1861 ccatggtcga cttgagccgc gagaaatggg tgaccggggt cgcctcggtc atcccgtagg
     1921 cgtggatgaa ctggaccccg tggcgctcct cgtaggcccg gatcatcgac accggggccg
     1981 ccgacccgcc gcagatgatc cgctgcaacg ggcccagatc gccgccgtcc ttctccagca
     2041 gctggagaat gccgagccag accgtcggca ccccggccga cacggtcacg ccctcctcgc
     2101 ggatgagccg caggtagtcg gccggggtcg gggccgggcc gccgtagacc tggccggccc
     2161 cgtaccaggt ggccgcgaac ggcatccccc aggcgttgac atggaacatg ggcacgatcg
     2221 gcaggagcac gtcccgctcg gagatggcgg ccgtgtcggc caggccgacc accagggagt
     2281 ggaggtacag ggcccggtgc gaataggcca cccccttggg gttgccggtg gtcgccgagg
     2341 tgtagcagat gccggccgtc tggccctcgt ccagggccgg ccagtcggtc atcggggccg
     2401 accgctcgac caggtcctcg tagccgcgct cgccggcgaa gccgagcgac ggccgctccc
     2461 cgtcctcggc gtccagcacc agcacgtgct cgacggtgtc gagctcgcca agcaccgggc
     2521 cgatcgcctg ggccagctcg gcgtcgcaga ccagcacctt gtcgcgggcg tggctgacga
     2581 tgtaggcgag ctggtcgccg gccaggcgga tgttcagggt gtggtagctg gcccccatca
     2641 gagcggccgc gaagtacacc tccaggtgcc ggtcgtggtt ccagcagaag gtggccaccc
     2701 ggtcgcccgg cccgacgccc agctcgcgca ggccgccggc cagccgggcc acccgctcgg
     2761 cccagtcgcc gtaggtggtc cgccgcacgc cccggggccg ccgggtgacc agcggcttgt
     2821 cggggaacag ggtgcgggac cgctcgtaga tggtccggac ggtgagcggg tggtccatca
     2881 tcgcgcggcc ctcctcactg gcggctgtcc agatgctacc gtcccgag
//
LOCUS       DASSWR010000684         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1269885, whole genome shotgun sequence.
ACCESSION   DASSWR010000684 DASSWR010000000
VERSION     DASSWR010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269885"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFO47_11500"
     CDS             complement(<1..677)
                     /locus_tag="VFO47_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789360.1"
                     /translation="MDTSDLVQSKLRPPLARPGIVSRSAVVDRLLAAPSAPLICVVAP
                     PGYGKSTVLAQWSGHKEPRAGWVSVDRRDNDPVVLLTYIAAALDRVEPVDPEVFQVLA
                     SPGVSVAATAVPRLVAAVSAMTRPVSLVLDHVELLENTECLDVVAELALRLPAGSQLA
                     VASRRAPALPVALLRAQGQVVEVGVTELAMDQGEARALLDATGVGLSDAEVTELAGRT
                     EGWPVGL"
     gene            855..2159
                     /locus_tag="VFO47_11505"
     CDS             855..2159
                     /locus_tag="VFO47_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET8789361.1"
                     /translation="MRSRLWLVLVAAALVLAACGNDSEAPSTAAQAPAATTSCASNGE
                     LTMWERSGGNKQMVDMLVEAWNTKNPNCKIKLTYIPHTEMVGKIAQGIASGEVPDLMG
                     MDLIYAPQFEKAQQLVDLTDRISTWPELKTASKGHMTVATFENRLYGVPLYADVSALF
                     YNKDLFKRAGLDPDKPPTSLAEMRAYADKITALGGDIKGYYLPGNCAGCNIFTVGPLM
                     WASGAKIEAAGPGDEPLVGDGVKQVLQFTRDMVKAGNVHEGDRTENGETFHLQFGSGK
                     VGMMGTGNFNITLARQQNPKMDFGIALLPGMTPNSSASFIGGDLVVVPKGSDRVDDAV
                     NFMKFLLSDEVQVEVYAKALNLTTRADMVENKYFEAEPLVQDVAKALTVGRTPYTLTF
                     FEQINSPQGPWLKMLQRAYYSDDNLDTVIADAKAAMKSIASQ"
     gene            2172..>2948
                     /locus_tag="VFO47_11510"
     CDS             2172..>2948
                     /locus_tag="VFO47_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8789362.1"
                     /translation="MYWGRRSRLTGIAYLAPAVLFVLAFTAYPLARMVWMSFHSWSLI
                     TPPSWVGLGNYRRAFADDQFWVSFAFTLKYTAIITPILIVGGYLLALLCSPGSPLRRL
                     TRTLVFVPVVIGLGVSSLLWYWLFSTDFGLINRALLDLGVISEPVLWLGVDADLSNLA
                     ITTSVVWKVIGFGMILFVGAIQAIPTEIHEASLVDGAGHWQRVTRVILPLTLRTVLVV
                     TLVSVIGSLLAFDQFYIMTAGQPQNETATSVFYVYLNSFPY"
BASE COUNT          486 a         1066 c          962 g          434 t
ORIGIN      
        1 tacagcccga ccggccagcc ctcggtccgg ccggccagct cggtcacctc ggcgtccgac
       61 agcccgacac cggtcgcgtc cagcagcgcc ctggcctcgc cctggtccat ggccagctcg
      121 gtcacgccga cctccaccac ctggccctgg gcgcgcagca gcgccaccgg cagcgccggc
      181 gcgcgccgcg aggcgacggc aagctgggac ccggccggga gccgcagcgc cagctcggcc
      241 accacgtcca ggcactcggt gttctcgagc agctccacgt ggtcgagcac cagggagacc
      301 ggccgggtca tcgccgagac cgccgccacc agccggggta cggccgtggc cgccaccgag
      361 acccctggcg aggcgaggac ctggaacacc tccgggtcga ccggctcgac ccggtccagc
      421 gcggccgcga tgtaggtcag cagcaccacc gggtcgttgt cgcgccggtc caccgagacc
      481 catcccgccc gcggctcctt gtggccgctc cactgggcca gcacggtgct cttgccgtag
      541 ccgggcgggg cgaccacgca gatgagcggc gccgacggcg cggcgagcag ccggtccacg
      601 acggcgctcc gggacacgat ccccgggcgg gccagagggg gccggagctt cgactgcacc
      661 agatcgctcg tgtccacccg acgagcgtag cccgcgtgcc tgccctgctc aacgagaggg
      721 ccagggcgac tcgacatgaa atcgaatgca tgccatcgta tgcataccgc gtatcgtccc
      781 cgaatgccca gagtccggta ggcgtcagcg tgagcccggt cgaaccgggc gagcccgaca
      841 tggaggagcg tgggatgagg agtcgtctgt ggctggtcct ggtggcggcg gcgctggtgc
      901 tcgccgcctg cgggaatgac agcgaagcac cgtcgaccgc cgcccaggcc ccggccgcca
      961 cgaccagctg cgccagcaac ggcgagctca cgatgtggga gcgctcgggc ggcaacaagc
     1021 agatggtcga catgctcgtc gaggcctgga acaccaagaa ccccaactgc aagatcaagc
     1081 tgacctacat cccccacacg gagatggtgg gcaagatcgc ccaggggatc gcctccggtg
     1141 aggtgcccga cctgatgggc atggacctca tctacgcccc gcagttcgag aaggcccagc
     1201 agctggtcga cctgaccgac cggatcagca cctggccgga gctgaagacg gccagcaagg
     1261 ggcacatgac ggtggccacc ttcgagaacc ggctctacgg ggtgccgctg tacgcggacg
     1321 tgtcggccct cttctacaac aaggacctct tcaagcgggc cggcctcgac cccgacaagc
     1381 cgcccacgag cctggccgag atgcgcgcct acgccgacaa gatcaccgcc cttggcggcg
     1441 acatcaaggg ctactacctg ccgggcaact gcgccggctg caacatcttc accgtcgggc
     1501 ccctgatgtg ggcctcgggg gccaagatcg aggccgccgg cccgggcgac gagccgctgg
     1561 tcggcgacgg ggtcaagcag gtcctgcagt tcaccaggga catggtcaag gccggcaacg
     1621 tccacgaggg cgaccggacc gagaacggcg agacgttcca cctccagttc gggtccggca
     1681 aggtgggcat gatgggcacc ggcaacttca acatcaccct ggcccgccag cagaacccga
     1741 agatggactt cggcatcgcc ctgctccccg ggatgacccc gaactcctcg gcctcgttca
     1801 tcggcggcga cctggtcgtg gtgcccaagg gcagcgaccg ggtggacgac gccgtcaact
     1861 tcatgaagtt cctgctctcg gacgaggtgc aggtcgaggt gtacgccaag gccctcaacc
     1921 tgaccacccg cgccgacatg gtcgagaaca agtacttcga ggccgagccg ctggtccagg
     1981 acgttgccaa ggcgctcacc gtcggccgca cgccgtatac gctgaccttc ttcgagcaga
     2041 tcaactcgcc ccagggaccg tggctgaaga tgctccagcg cgcctactac agcgacgaca
     2101 acctcgacac cgtcatcgcc gacgccaagg cggcgatgaa gagcatcgcc agccaatagg
     2161 ccacgatcgc cgtgtactgg ggccgacgga gccggctgac cgggatcgcg tacctggcgc
     2221 cggccgtgct cttcgtgctg gccttcaccg cctacccgct ggcccggatg gtgtggatgt
     2281 cgttccacag ctggtcgctg atcacgccgc cgtcctgggt cgggctcggc aactaccggc
     2341 gggcgttcgc cgacgaccag ttctgggtct cgttcgcctt cacgctcaag tacacggcga
     2401 tcatcacgcc gatcctgatc gtcggcggct acctgctggc cctgctctgc tcgccgggct
     2461 cgccgctgcg gcggctcacc cggacgctcg tgttcgtccc cgtggtcatc ggcctgggcg
     2521 tgtccagcct gctctggtac tggctcttca gcaccgactt cggcctgatc aaccgggccc
     2581 tgctcgacct gggcgtgatc tccgagccgg tgctgtggct gggggtcgac gccgacctgt
     2641 cgaacctggc catcaccacc tcggtcgtct ggaaggtgat cggcttcggg atgatcctgt
     2701 tcgtcggcgc catccaggcc atccccaccg agatccacga ggccagcctg gtcgacgggg
     2761 ccggccactg gcagcgggtg acccgggtga tcctgcctct gaccctacgc acggtgctgg
     2821 tggtgacgct ggtgagcgtc atcgggtcgc tgctcgcctt cgaccagttc tacatcatga
     2881 ccgccgggca gccgcagaac gagaccgcga cctcggtctt ctacgtctac ctgaactcct
     2941 tcccctac
//
LOCUS       DASSWR010000685         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_308524,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000685 DASSWR010000000
VERSION     DASSWR010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308524"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFO47_11515"
     CDS             <1..199
                     /locus_tag="VFO47_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789363.1"
                     /translation="EQTEDRSMSAFIAELNRLGEDGWEAVGITPRTHYDRGGGPGGWD
                     TFTFVVLLKRLRPADDMMPRS"
     gene            204..692
                     /locus_tag="VFO47_11520"
     CDS             204..692
                     /locus_tag="VFO47_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789364.1"
                     /translation="MKGAIRVLVVGATAGLVAGQVMDKATTWYYGRQSDASRQREQEL
                     LPEGAPLAAARKLAGLVGAEPTDDQAATIALAMHRGLGQGYGVAAAALTQRGVPPLAA
                     GVAAGMAGFLVVDELANSLFFTPPPQAYPVESHLRGVVGHLTLGVVTGVLLALARRLR
                     LL"
     gene            complement(689..1993)
                     /gene="glcF"
                     /locus_tag="VFO47_11525"
     CDS             complement(689..1993)
                     /gene="glcF"
                     /locus_tag="VFO47_11525"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HET8789365.1"
                     /translation="MAVLPAFDDHHPPEQELIDDCVHCGFCLPTCPTYVLWGEEMDSP
                     RGRIYLMELGRQGEALNTEMVRHFDQCLGCMACVTACPSGVQYGKLIEATRQQVERRY
                     PRPRRDRAFRALIFRLFPYPERLRLLRGPLRLYQRARVGALVRRAGLLPGRLGAMERL
                     LPDLGRPEPVPEVTPAEGTHRRRVGMLTGCVQRVFFPEVNAATARVLAADGCEVVAPP
                     GQGCCGALSLHAGREAEAQAFARAAIDTFDAAGVDQVVVNAAGCGSAMKEYGHLLRDD
                     PAWAERAQAFSDRVRDLSELLVELGPVAPRHPLPLKVAWHDACHLGHAQGIRAQPRAA
                     LRAIPGLEVREIAEGEICCGSAGIYNLVEPEPAAELGDRKAANVLATGADLVVTSNPG
                     CLMQLRNALERRGQRLPMAHVAEVLDAAVRGAQPGSLLERRS"
BASE COUNT          251 a          738 c          795 g          232 t
ORIGIN      
        1 cgagcagacc gaggaccggt cgatgagcgc gttcatcgcc gagctcaacc ggctcgggga
       61 ggacggctgg gaggcggtcg ggatcacgcc ccggacccac tacgaccgcg ggggcgggcc
      121 gggcggctgg gacaccttca ccttcgtggt actcctgaag cggctgcggc cggccgacga
      181 catgatgccg aggagctgag gccatgaagg gcgcgatccg ggtcctggtc gtgggcgcca
      241 ccgccgggct cgtcgccggc caggtgatgg acaaggcgac gacctggtac tacgggcgcc
      301 agagcgacgc ctccaggcag cgcgagcagg agctgctgcc cgagggcgcc ccgctggcgg
      361 cggcccggaa gctggccggc ctggtcgggg ccgagccgac cgacgaccag gccgccacga
      421 tcgccctggc catgcaccgg gggctggggc agggctacgg ggtggccgcg gccgcgctca
      481 cccagcgcgg cgtcccgccc ctggccgccg gggtcgccgc cggcatggcc gggttcctgg
      541 tggtggacga gctggccaac agcctgttct tcaccccgcc gccccaggcc tacccggtcg
      601 agagccacct gcgcggcgtc gtcggccacc tcaccctcgg agtcgtcacc ggcgtgctgc
      661 tggccctggc ccggcggctg cggctgctct agctccggcg ctccagcagc gagccgggct
      721 gggcgccgcg gacggccgcg tccaggacct cggccacatg cgccatgggc aggcgctggc
      781 cgcggcgctc cagggcgttg cgcagctgca tgaggcagcc cgggttggag gtgaccacca
      841 ggtcggcccc ggtggcgagc acgttggccg ccttgcggtc gcccagctcg gccgccggct
      901 ccggctcgac caggttgtag atgccggccg agccgcagca gatctcgccc tcggcgatct
      961 cgcgcacctc caggcccggg atggcccgca gggccgcccg gggctgggcc cggatgccct
     1021 gggcgtggcc caggtggcag gcgtcgtgcc aggccacctt gagcggcagc gggtgccggg
     1081 gggcgaccgg gccgagctcc accagcagct cggacaggtc gcggacccgg tcggagaacg
     1141 cctgggcccg ctccgcccag gccgggtcgt cgcggagcag gtggccgtac tccttcatgg
     1201 ccgagccgca gccggccgcg ttgaccacca cctggtcgac cccggcggcg tcgaaggtgt
     1261 cgatggccgc cctggcgaac gcctgggcct cggcctcgcg gccggcatgg aggctgaggg
     1321 cgccgcagca gccctggccg ggcggggcca cgacctcgca gccgtcggcg gccaggaccc
     1381 gggccgtggc cgcgttgacc tcggggaaga acacccgctg gacgcagccg gtgagcatcc
     1441 cgacccgccg tctgtgcgtc ccttcggccg gggtgacctc gggcaccggc tccgggcggc
     1501 ccaggtcggg cagcagccgc tccatggccc cgaggcgccc gggcagcagc ccggcccgcc
     1561 ggaccagggc gcccacgcgg gcccgctggt agaggcggag ggggccgcgc agcaggcgca
     1621 gccgctccgg gtaggggaac agccggaaga tcagcgcccg gaaggcgcgg tcgcgccgcg
     1681 ggcgcgggta gcggcgctcg acctgctggc gggtggcctc gatcagcttg ccgtactgga
     1741 ccccggacgg gcaggcggtg acgcaggcca tgcagccgag gcactggtcg aagtggcgga
     1801 ccatctcggt gttcagggcc tcgccctggc gccccagctc catcaggtag atgcggcccc
     1861 ggggcgagtc catctcctcg ccccagagca cgtaggtcgg gcaggtgggc aggcagaagc
     1921 cgcagtggac gcagtcgtcg atcagctcct gctcgggcgg gtggtggtcg tcgaaggcgg
     1981 ggaggacggc caccccggcc tcgggccggc cggccc
//
LOCUS       DASSWR010000686         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_449989,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000686 DASSWR010000000
VERSION     DASSWR010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449989"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..546
                     /locus_tag="VFO47_11530"
     CDS             145..546
                     /locus_tag="VFO47_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018737176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789366.1"
                     /translation="MFFTKLLRALAGLLLAGLLAAACADPGDDGAGAGQGPATTAPTT
                     PPTTAPPTSGTTPAPPAGAVTVSGTVTPGVEGNCLLLEAKGGPYLLIGGDRAKLRPGA
                     RVAVTGRVDRDLLSICQQGVPLVVASIEPAP"
     gene            complement(550..1203)
                     /locus_tag="VFO47_11535"
     CDS             complement(550..1203)
                     /locus_tag="VFO47_11535"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET8789367.1"
                     /translation="MPSTRYPRRGDNDDFGRVVYFTDAVFAIAMTLLVVEIGVPETIA
                     GGADDPGALLDAFADKGPLVFAFFLGCYVIGSYWVAHHRFMSWLSAVDRGFIALTVVY
                     LSFVALLPFPTGVLGEFGENPTSVVAFALNMGAVSTMEAVLFRHARRRRLFRQEWPED
                     VYRWALLSSLSPVLMFTLSVPVAFVDARLAIVMWFLAIPLGMLLGRRRPAEAARYVA"
     gene            1293..1433
                     /locus_tag="VFO47_11540"
     CDS             1293..1433
                     /locus_tag="VFO47_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789368.1"
                     /translation="MGPGSGDLVGATGIAAQMVGCWGGGPTLLASRPAPAGELADPGQ
                     AG"
     gene            1458..1769
                     /locus_tag="VFO47_11545"
     CDS             1458..1769
                     /locus_tag="VFO47_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789369.1"
                     /translation="MATQYPDDDLPREPDPPARATDVDKQDPETPDHIPEPEASEVES
                     ARLLANQARPELGAEGFSDQRIDELASEFIARDIGEGLEEVLRWARAEGPLPQGSGEI
                     F"
     gene            complement(1740..2582)
                     /locus_tag="VFO47_11550"
     CDS             complement(1740..2582)
                     /locus_tag="VFO47_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8789370.1"
                     /translation="MRLAQEVAREVVGHVRRHDLVFYAAGLTFYAGIAIVPLLLVAWW
                     VTSLVLGDDLVRTLTLALAEYAPTSLGFQEGVQALGEVGPRLGIASFAAALIPATSYG
                     DGLVRALDRIAERNRRAKGLRGRLLGLVFVATLPPVVMAELGAVAVLPGALGFTGRFN
                     LLSVAVAFVVGWLSATLLVGVLYRAFSPRPIQAGPLALGAALTGSFLSGMSLTWLFLL
                     RFGVDVGIAFGDSDLLGTVVVAAVYLYLVQVVLLGGYVLSLVLARFAARAGTRSEDLA
                     AALR"
BASE COUNT          450 a         1136 c         1072 g          327 t
ORIGIN      
        1 gtcaggggca tgggcggcca ggtctgggtc gaggacaacc cgggcggcgg cgcccgcttc
       61 gtggtcgagc tgcccgacct ggccccggta tagggtcgcc ccggacgccg ggaacctccc
      121 ggccccctgg ggcgtccaaa cggcatgttc ttcacgaagc tcctgcgcgc gctggccggc
      181 ctgctcctgg ccgggctgct ggccgccgcc tgcgccgacc ccggggacga cggcgccggg
      241 gccggccagg gccccgccac caccgcgccc acaaccccgc ccacgaccgc cccgcccacc
      301 agcggaacga ccccggcccc gcccgccggc gcggtcaccg tctccggcac cgtcaccccc
      361 ggggtcgagg gcaactgcct gctcctggag gccaagggcg ggccctacct gctcatcggc
      421 ggcgaccggg cgaagctgcg gcccggcgcc cgggtcgcgg tcaccggccg ggtcgaccgc
      481 gacctgctca gcatctgcca gcagggcgtg ccgctggtgg tggcgtcgat cgaaccggcg
      541 ccctgaccct caggcgacgt accgggccgc ctccgccggc cggcgccggc ctaggagcat
      601 ccccaacggg atggccagga accacatcac gatcgccagc cgggcgtcga cgaaggcgac
      661 cggcacggac agcgtgaaca tcagcaccgg cgacagcgac gacagcaggg cccagcggta
      721 gacgtcctcc ggccactcct gccggaacag ccgccgacgc ctggcatggc ggaacagcac
      781 cgcttccatg gtgctgaccg cccccatgtt cagggcgaag gcgaccaccg aggtcgggtt
      841 ctccccgaac tcgcccagca cgccggtggg gaagggcagc agggccacga acgacaggta
      901 gacgacggtc agggcgatga agccccggtc gaccgcgctc agccacgaca tgaagcggtg
      961 gtgcgccacc cagtaggagc cgatcacgta gcagccgagg aagaacgcga agaccagcgg
     1021 ccccttgtcg gcgaaggcgt ccagcagggc gccggggtcg tcggcgcccc cggcgatcgt
     1081 ctcgggcacg ccgatctcca ccaccagcag ggtcatggcg atcgcgaaca cggcgtcggt
     1141 gaagtagacg acccgtccga agtcgtcgtt gtcgccacga cgtggatagc gggtcgacgg
     1201 catcggcggc tccctccgcg ctcggtgacg gccacgcccc atcatcgacc cgatgccgcg
     1261 acccgggtgg ggacgcccgc ttcgccgggc tggtgggtcc gggcagcggc gacctggtcg
     1321 gggccaccgg catcgccgcc cagatggtgg ggtgctgggg cggcgggccc accctgctcg
     1381 ccagccggcc cgcgccagcc ggtgagctgg ccgatccggg ccaggccggg taggttcacc
     1441 ccggaggtga gctgaccttg gcgacccagt accccgacga cgacctgcct cgcgagcccg
     1501 acccgccggc gcgcgcgacc gacgtcgaca agcaggaccc ggagaccccc gaccacatcc
     1561 ccgagcccga ggcctcggag gtcgagagcg cgcgcctgct ggccaaccag gcccggcccg
     1621 agctcggcgc cgagggcttc tccgaccagc gcatcgacga gctggcctcg gagttcatcg
     1681 ccagggacat cggcgagggc ctcgaggagg tcctccgctg ggcccgcgcc gaaggcccgc
     1741 taccgcaggg cagcggcgag atcttctgac ctggttccgg ctcgggcggc gaaccgggcc
     1801 aggaccaggc tgaggacgta gccgccgagc aggaccacct ggaccaggta caggtagacg
     1861 gcggccacga ccacggtgcc gagcaggtcg gagtcgccga aggcgatgcc gacgtcgacc
     1921 ccgaagcgga gcaggaacag ccaggtcagc gacatgccgg agaggaacga cccggtcagg
     1981 gccgccccaa gggcgagcgg cccggcctgg atcggccgcg gcgagaaggc ccggtagagg
     2041 acgcccacca gcagggtcgc cgacagccac ccgaccacaa aggccacggc cacgctgagc
     2101 aggttgaagc ggccggtgaa cccgagggcg ccgggcagca ccgccaccgc ccccagctcg
     2161 gccatgacca ccggcggcag cgtggccacg aacaccagcc ccagcagccg cccgcgcagg
     2221 cccttggccc ggcggttccg ctcggcgatc cggtccagcg cccgcaccag cccgtccccg
     2281 tagctggtcg ccgggatcag ggcggccgcg aacgaggcga tccccagccg cggcccgacc
     2341 tcgccaaggg cctgcacccc ctcctgaaac ccgagcgacg tcggcgcgta ctcggccagg
     2401 gcgagggtca gggtccggac caggtcgtcg ccgagcacca gcgaggtgac ccaccaggcc
     2461 accagcagca gcggcacgat cgcgatgccc gcgtagaagg tcagcccggc ggcgtagaac
     2521 accaggtcgt gccgccgcac atggcccacg acctcgcggg ccacctcctg cgcgagccgc
     2581 acgctccgac ccgccacgtc gtcgttccct tcagccggct gtcctgtcac ctggttcttc
     2641 gcggtgcatc ggcccggcgg ttgcggtccg gagcagggtg gtgtcatggg ggcggggggt
     2701 gtggatggct ggttgtccgg gtttctgggc gcggatacaa ctcgttccgg ccagatcgcc
     2761 gatcgtcgcg ccagttccgt tgcgccgtcc cgtcgccgtc gtgccgagag gagccgtccg
     2821 atgccgtacc gtccgccgtc gccggcgacc cttgaccgat gagccgtccg gcgggggagc
     2881 cggccgccct ggccgcgtgt tcccggagca tgggcggtgc cgccgccgcg ctgcgggagg
     2941 tccgcgcgcg gctgggccgc acgacctcgt ccgaggtcgt ggccg
//
LOCUS       DASSWR010000687         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_108913,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000687 DASSWR010000000
VERSION     DASSWR010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108913"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFO47_11555"
     CDS             <1..1136
                     /locus_tag="VFO47_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8789371.1"
                     /translation="YAAAQQELVERQRAELRFRTLVEQLPVMVYSEEFEVGGSRMWVN
                     SQVAMFGASLEDASSKNFWKTRLHPDDRDRVLAEEARCEQTGEPFRMEYRTIHADGHV
                     MWIRDQCVLVRDESGQPLYWQGVVVDLTDVRRALELEREATRRLRALDQMKNTFLDAV
                     SHELRTPLAAIVGIGLTLEHKAGDMAEEDRRDLYTRLVANGRKLDRLLNDLLDLDRLT
                     HGIVAPKRRPTDVAALATRIADDWALLNGRRPQVVAEPVTVSLDPAKVERIIENLLAN
                     AARHTPADTPVWVRVEQPPGGDGVLLAVEDAGAGVPAELRDSVFEPFRQGPGTPAHAP
                     GVGIGLTLVARFAELHGGRAWVEERPGGGSSFRVLLPDAPQEG"
     gene            1186..1701
                     /locus_tag="VFO47_11560"
     CDS             1186..1701
                     /locus_tag="VFO47_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET8789372.1"
                     /translation="MVERALVLNASYQPICVVPVRRAVVLVLKEKADVLVPGSGLVRG
                     ATLEVQAPSVIRLRYFVKVPFRARAGLSRRAVFVRDDHTCQYCGERAENVDHVVPRSR
                     GGLHVWENVVAACRRCNSQKEDRLLHEAGLRLRREPRAPKETLWIVIAVGRIEPGWAP
                     FLGLEADAVPA"
     gene            1620..3227
                     /locus_tag="VFO47_11565"
     CDS             1620..3227
                     /locus_tag="VFO47_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET8789373.1"
                     /translation="MDRDRRRPHRTWLGAVPRPGGRRRPRLTVPPSTRASLAAFARRI
                     APWALPLLAGALGALLAVTFLARQEVTVGPARVRLEAGPAVTGSSRLAAPPFGSVSAR
                     THQGPLALRATIDDIDVQGLGRVLEGSPGPPGPGGPRFAELEATLGPLESQARRAATV
                     FVARIAVLGLAGGLAGVLLFRRRTRQRLLRCSLGGLLATVGLLGPALATYDLTAFREP
                     RYDGALEYAPALIGDVRTGLDRLRTLREEMVRVGRNLDRAYAALANPVGELDGNGTVR
                     VLHVSDLHLNPAGFDLAERLATQFDVAAVVDTGDMGTWGLPTEPAVATNIGRFRVPYL
                     FVKGNHDDADMVEAVAANDNARVLDGGGAEVAGIRFFGVADPTFTPGKGHRVEEFEEF
                     KVERSVGVADAIERQALRPDVLLVHDGRLAAYARGHVATVLEGHLHRFGTEVVNGTRT
                     LMTGTTGAGGPDNFRAADPPPADAQVVYFDPETRRPVAVDRITVRLPGSSFSVERLLL
                     PEGATPFTPDPLEVPPDLVPAPGAQGG"
     gene            3390..>3593
                     /locus_tag="VFO47_11570"
     CDS             3390..>3593
                     /locus_tag="VFO47_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET8789374.1"
                     /translation="MEGTGEGAALFIGTYDHSLDPKGRVILPRKFRDELGQDMVITKG
                     VGKERCLYVFPQAEFEAFAAKLNS"
BASE COUNT          458 a         1374 c         1273 g          488 t
ORIGIN      
        1 tgtacgccgc cgcccagcag gagctggtcg agcgccagcg ggccgagctg cgcttccgca
       61 ccctggtcga gcagctcccg gtgatggtct acagcgagga gttcgaggtc gggggctccc
      121 gcatgtgggt caactcccag gtggcgatgt tcggggcctc gctcgaggac gcctccagca
      181 agaacttctg gaagacccgc ctgcaccccg acgaccgcga ccgggtgctg gccgaggagg
      241 cccgctgcga gcagaccggc gagccgttcc ggatggagta ccggaccatc cacgccgacg
      301 ggcacgtcat gtggatccgc gaccagtgcg tgctcgtccg cgacgagtcc ggccagcccc
      361 tgtactggca gggggtggtg gtcgacctca ccgacgtccg ccgggccctg gagctggagc
      421 gggaggcgac ccggcggctg cgcgccctcg accagatgaa gaacaccttc ctggacgcgg
      481 tgtcgcacga gctgcgcacc cccctggccg cgatcgtcgg catcggcctc accctggagc
      541 acaaggccgg cgacatggcc gaggaggacc gccgcgacct ctacacccgg ctggtggcca
      601 acggccgcaa gctcgaccgg ctgctcaacg acctgctcga cctggaccgg ctgacccacg
      661 ggatcgtcgc ccccaagcgc cgcccgaccg acgtggccgc cctggcgacc cggatcgccg
      721 acgactgggc cctgctcaac ggccgccggc cccaggtcgt ggccgagccc gtcaccgtct
      781 ccctcgaccc ggccaaggtc gagcggatca tcgagaacct gctggccaac gcggccaggc
      841 acaccccggc ggacaccccg gtgtgggtgc gggtggagca gccgcccggc ggcgacggag
      901 tcctgctggc ggtcgaggac gccggcgccg gcgtcccggc cgagctgcgc gacagcgtgt
      961 tcgagccgtt ccgccagggg ccgggcaccc cggcccacgc ccccggggtc ggcatcgggc
     1021 tgaccctggt cgcccggttc gccgagctcc acggggggcg ggcctgggtg gaggagcggc
     1081 cggggggcgg ctcgtcattc cgggtcctgc tcccggacgc gccccaggaa ggctgacctc
     1141 caccgttata ctgctgagcg gccccacgca tcgccacggg agggcatggt ggagcgagcg
     1201 ctcgtcctca acgcgtcgta ccagccgatc tgcgtcgtgc cggtgcggcg ggcggtggtg
     1261 ctggtgctca aggagaaggc cgacgtgctc gtgcccggca gcggcctggt ccgcggggcc
     1321 accctggagg tccaggcccc cagcgtcatc cggctccggt actttgtgaa ggtgccgttc
     1381 cgagcccggg ccgggctctc ccgtcgagcg gtgttcgtgc gcgatgacca cacctgccag
     1441 tactgcggcg agcgagccga gaacgtcgac cacgtggtgc cccgctcccg tggcggcctc
     1501 cacgtctggg agaacgtggt cgccgcctgc cggcgctgca acagccagaa ggaggaccgc
     1561 ctcctccatg aggcaggcct ccgcctgcgg cgcgagcccc gggcgcccaa ggagacgctt
     1621 tggatcgtga tcgccgtcgg ccgcatcgaa cctggctggg cgccgttcct cggcctggag
     1681 gccgacgccg tccccgcctg accgtcccgc cctcgacccg ggcgtccctc gccgccttcg
     1741 ccaggcgcat cgccccatgg gcgctgcccc tgctcgccgg ggcgcttggc gcgctgctgg
     1801 ccgtcacctt tctggcccgg caggaggtca ccgtcgggcc ggcccgggtg cggctggagg
     1861 ccggcccggc cgtgaccggg tcgagccggc tggccgcgcc gccgttcggg tcggtctcgg
     1921 cccggaccca ccagggaccg ctcgccctgc gggccaccat cgacgacatc gacgtccagg
     1981 ggctggggcg ggtgctggag ggcagcccgg gtccccccgg gcccggcggg ccccgcttcg
     2041 ccgagctgga ggcgaccctc ggccccctgg agagccaggc ccggcgggcc gccaccgtgt
     2101 tcgtggcccg gatcgccgtg ctcgggctgg ccggcgggct ggccggggtg ctgctgttcc
     2161 gccgccggac ccgccagcgg ctgctgcgct gcagcctcgg cggcctcctg gccaccgtcg
     2221 ggctgctggg accggccctg gccacctacg acctgaccgc cttccgcgag ccccgctacg
     2281 acggcgccct ggagtacgcc ccggccctga tcggcgacgt ccgcaccggc cttgaccggc
     2341 tgcgcaccct gcgcgaggag atggtccgcg tcggccgcaa cctggaccgg gcctacgccg
     2401 ccctggccaa cccggtcggt gagctcgacg gcaacggcac cgtccgggtc ctgcacgtct
     2461 ccgacctgca cctcaacccg gccgggttcg acctggccga gcggctggcc acccagttcg
     2521 acgtggccgc ggtggtcgac accggcgaca tgggcacctg gggcctgccg acggagccgg
     2581 cggtggccac caacatcggc cgcttccggg tgccgtacct gttcgtcaag ggcaaccacg
     2641 acgacgccga catggtcgag gccgtggccg ccaacgacaa cgcccgggtg ctggacggcg
     2701 gcggcgccga ggtcgccggg atccgcttct tcggggtcgc cgatccgacc ttcaccccgg
     2761 gcaagggcca ccgggtggag gagttcgagg agttcaaggt ggagcggagc gtcggcgtgg
     2821 ccgacgccat cgaacgccag gccctgcgcc ccgacgtcct cctggtccac gacggccgcc
     2881 tggccgccta cgcccgcggg cacgtcgcca ccgtcctcga gggccacctc caccgcttcg
     2941 gcaccgaggt cgtcaacggc acccgcaccc tgatgaccgg gaccaccggg gccggcggcc
     3001 ccgacaactt ccgggccgcc gacccgcccc cggccgacgc ccaggtcgtc tacttcgacc
     3061 ccgagacccg ccgcccggtg gccgtcgacc gcatcaccgt ccgcctcccc ggctcctcct
     3121 tctcggtcga gcgcctgctc ctgcccgagg gcgcgacgcc cttcaccccc gaccccctgg
     3181 aggtcccccc ggacctcgtc ccggcccccg gcgcccaggg cggctgaccc tctctccctc
     3241 gctccagtgg agggaagtgg aggattggcg gtcgttccgg tctggccctg atttcggtcg
     3301 ctgatcccca ttttcccagc tcacctggcc tctctctgtg gctgcatatg cattgacggt
     3361 gggggagcgg tggagtaaag tggcgccaag tggagggaac gggggaggga gcggcgctgt
     3421 tcatcggcac ctacgaccac tccctggatc cgaagggccg ggtgatcctt ccgcgcaaat
     3481 tccgggacga gctggggcag gacatggtga tcaccaaggg cgtcggcaag gagcggtgcc
     3541 tgtacgtgtt cccccaggcc gagttcgagg ccttcgcggc caagctcaac agc
//
LOCUS       DASSWR010000688         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_168078,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000688 DASSWR010000000
VERSION     DASSWR010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168078"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2424)
                     /locus_tag="VFO47_11575"
     CDS             complement(67..2424)
                     /locus_tag="VFO47_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET8789375.1"
                     /translation="MTGDSAPTRAAADSHDLIQVRRARENNLANVSLDIPKRRLTVFT
                     GVSGSGKSSLVFGTIAAESRRLINETYTAFVQSFMPNLARPDVDALHNLSAAIIVDQE
                     RMGANARSTVGTATDAYTMLRIVFSRLGQPYVGPSTAFSFNMPAGQARGEVELERGDG
                     ATVSREVTLAGGMCPECEGLGRVSAIDVDQLVDRERSLNEGAITVPNFGVGTWYWKSL
                     AEAGFVDPDTKLKDYTPTQWEDFLHKPATKIKLAGINVTYEGLVVKVQRLYLAKDRES
                     MQPHIRAFVDRAVTFRPCPACGGTRLNPAALSSKVKGRNIAECSAMQVSDLASFVRDI
                     EDASVGPLVASLRETLDSMVEVGLGYLSLDRESGTLSGGEAQRVKMVRHLGSALTDVT
                     YVFDEPTVGLHPHDVQRMNDLLLRLRDKGNTVLVVEHEPEVIEVADHVVDLGPGAGSA
                     GGTVCFAGDVAGLRRSGTLTGRHLDHRTPLRERVRAPKGQLSITGASLHNLRDVSVDI
                     PLGVLTVVTGVAGSGKSSLVHGSLAGREGVTIVDQSPIRGSRRSNPATYTGVLDPIRS
                     AFAKANGVKASLFSANSEGACPNCNGIGLVYTDLAMMAGVATVCEQCEGRRFTPEVLT
                     YKLDGKDISEVLAMSVTEARDFLTARPARVILDRLADVGLGYLGLGQPLTTLSGGERQ
                     RLKLAIHMAAAATTYVLDEPTTGLHLADVENLLGLLDRIVDSGRSVIVIEHDQAVMAH
                     ADWIIDLGPGAGHDGGRVVFTGTPADLVAGADTLTARHLRAYLRR"
BASE COUNT          345 a          858 c          971 g          377 t
ORIGIN      
        1 caacgtccgg gtccgggccc ccgacgggat gcagctcacc ctgttcaccg tgctcgaggg
       61 gaccggctag cgccgcaggt aggctcgcag gtgccgggcg gtgagggtgt cggcgccggc
      121 gaccaggtcg gcgggggtgc cggtgaagac cacccggccg ccgtcgtggc cggcgcctgg
      181 gccgaggtcg atgatccagt cggcgtgcgc catgaccgcc tggtcgtgct cgatgacgat
      241 gaccgacctg ccggagtcga cgatccggtc gagcaggccg agcaggttct cgacgtcggc
      301 caggtggagg ccggtggtcg gctcgtcgag cacgtaggtg gtggccgcgg cggccatgtg
      361 gatggccagc ttgagccgct gccgctcgcc ccccgacagg gtggtgagcg gctggccgag
      421 gccgaggtag ccgaggccga cgtcggccag gcggtcgagg atgacccggg ccgggcgggc
      481 agtcaggaag tcgcgggcct cggtcaccga catggccagg acctcgctga tgtccttgcc
      541 gtcgagcttg taggtcagca cctcgggggt gaaccgccgg ccctcgcact gctcgcagac
      601 ggtcgccacc ccggccatca tggccaggtc ggtgtagacc aggccgatgc cgttgcagtt
      661 ggggcaggcg ccctcggagt tggcgctgaa cagggacgcc ttgaccccgt tggccttggc
      721 gaaggcgctg cggatggggt cgagcacccc ggtgtaggtg gccgggttgc tgcggcggga
      781 cccgcggatg ggggactggt cgacgatggt caccccctcg cggccggcca gggagccgtg
      841 gaccagcgag ctcttgcccg acccggccac gccggtgacc acggtgagca ccccgagggg
      901 gatgtcgacg ctcacgtccc tgaggttgtg cagggacgcg ccggtgatcg acagctggcc
      961 cttgggggcc cggacccgct cgcgcagggg cgtgcggtgg tccaggtgcc tgccggtcag
     1021 cgtgcccgac cggcgcaggc cggccacgtc gccggcgaag cagaccgtcc ccccggccga
     1081 ccccgccccc gggcccaggt cgaccacgtg gtcggcgacc tcgatgacct cgggctcgtg
     1141 ctcgaccacc aggacggtgt tgcccttgtc gcgcaggcgc agcagcaggt cgttcatgcg
     1201 ctggacgtcg tggggatgga ggccgacggt gggctcgtcg aagacgtagg tgacgtcggt
     1261 cagggccgac ccgaggtggc ggaccatctt gacccgctgg gcctcgcccc cggacagggt
     1321 gccggactcg cggtccaggc tcaggtagcc gaggccgacc tcgaccatcg agtcgagcgt
     1381 ctcccgcagg gaggccacca gcggccccac cgaggcgtcc tcgatgtcgc ggacgaagga
     1441 ggccaggtcg ctgacctgca tggccgagca ctcggcgatg ttgcggccct tgaccttgga
     1501 ggacagggcg gccgggttga gccgggtgcc gccgcaggcc gggcagggcc ggaaggtcac
     1561 ggcccggtcg acgaaggccc ggatgtgggg ctgcatcgac tcccggtcct tggccaggta
     1621 gagccgctgc accttcacga ccaggccctc gtaggtgacg ttgatgccgg ccagcttgat
     1681 cttggtggcc ggcttgtgga ggaagtcctc ccactgggtg ggggtgtagt ccttgagctt
     1741 ggtgtcgggg tcgacgaagc cggcctcggc caggctcttc cagtaccagg tgccgacgcc
     1801 gaagttgggg acggtgatgg cgccctcgtt gagcgagcgc tcccggtcga ccagctggtc
     1861 gacgtcgatc gccgacaccc ggccgagccc ctcgcactcg gggcacatgc cacccgccag
     1921 cgtgacctcc cgcgagaccg tggcaccgtc cccgcgctcc agctcgacct cgccgcgggc
     1981 ctgcccggcg ggcatgttga acgagaaggc ggtcgagggg ccgacgtacg gctggcccag
     2041 ccggctgaag acgatccgca gcatggtgta ggcgtcggtg gcggtgccga ccgtcgagcg
     2101 ggcgttggcg cccatccgct cctggtcgac gatgatcgcc gcgctcaggt tgtgcagggc
     2161 gtcaacgtcg ggccgggcca ggttgggcat gaacgactgg acgaaggccg tgtaggtctc
     2221 gttgatcagc cgccgtgact cggcggcgat ggtcccgaac accagcgagg acttgcccga
     2281 cccggagacg ccggtgaaca ccgtcagccg ccgcttgggg atgtccagcg agacgttggc
     2341 caggttgttc tccctggccc ggcggacctg gatgaggtcg tggctgtcgg cggcggcccg
     2401 ggtgggtgcg ctgtcgcctg tcatgccgat ccctcatcct gttctggccg gggacccgat
     2461 tatcccccgg acccgtgacc cggaggccac catgcccgac cacgccgcca cggccagccg
     2521 gttcctggag ctgcaccacc aggggcggcc g
//
LOCUS       DASSWR010000689         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_251936,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000689 DASSWR010000000
VERSION     DASSWR010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251936"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VFO47_11580"
     CDS             complement(<1..1226)
                     /locus_tag="VFO47_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8789376.1"
                     /translation="MLASAGLMFLKSLTLRGFKSFAEKTTLDFEPGITVIVGPNGSGK
                     SNVVDAVAWVLGEQGPKSLRGGKMEDVIFAGTGSRGALGRAEVALTIDNSAGLLPIEY
                     SEVTVTRLLYRSGESDYAINGTTCRLLDIQELLSDTGVGRELHTVLGQNRLEDVLGGR
                     PEDRRAAIEEAAGVYKHRRRKEKALRKLAGMEQHLVRLSDLVGELRRQLKPLQQQAET
                     AARAAEVAAELREVRLTLRARELAGVRQRSASLDAEEAAFAARLAELEQRHDAEEARE
                     RGYQAELAELEPQTGRATDAVHRLATVRERLRGTVALAEARARHLAESLATEHEGRSV
                     ADLEREAAEVAAELARVEDELAATTATATEAAAVRNRARAALAAFDQAAARAERDRAT
                     AHRREVRLASEVAGLER"
     gene            complement(1317..1502)
                     /locus_tag="VFO47_11585"
     CDS             complement(1317..1502)
                     /locus_tag="VFO47_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789377.1"
                     /translation="MSKALMGYVGQPPAYMILRELNALKARVAELERHLAVAEQELAL
                     REVEDFRPSTTKEAALA"
     gene            complement(1791..2039)
                     /locus_tag="VFO47_11590"
     CDS             complement(1791..2039)
                     /locus_tag="VFO47_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET8789378.1"
                     /translation="MRGEVQGVGFRWAVQRQAGQLGLTGYAENLPDGSVRVEAEGDPD
                     RLDQLESFLREGPRWAEVESLDSQRIPATGEFHYFEAR"
BASE COUNT          261 a          739 c          764 g          296 t
ORIGIN      
        1 cgctccagcc cggcgacctc gctggccagg cggacctcgc gccggtgggc ggtggcccgg
       61 tcccgctcgg cccgggcggc ggcctggtcg aaggcggcca gggcggcccg ggcgcggttg
      121 cggacggccg cggcctcggt cgcggtggcg gtggtcgcgg ccagctcgtc ctcgacccgg
      181 gcgagctccg cggcgacctc ggccgcctcc cgttccaggt cggccacgga gcggccctcg
      241 tgctcggtgg ccagcgactc ggccaggtgg cgggcacggg cctcggccag ggcgaccgtg
      301 ccccggaggc gctcgcgcac ggtggccagg cggtgcacgg cgtcggtggc ccggccggtc
      361 tggggctcca gctcggccag ctcggcctgg tagccgcgct cgcgcgcctc ctcggcgtcg
      421 tggcgctgct ccagctcggc cagccgggcg gcgaaggcgg cctcctcggc gtccagcgac
      481 gccgagcgct ggcggacccc ggccagctcc ctggcccgca gggtgaggcg gacctcgcgc
      541 agctcggcgg cgacctcggc cgccctggcc gccgtctcgg cctgctgctg cagcggcttg
      601 agctggcggc gcagctcccc gacgaggtcg gacagcctga ccaggtgctg ctccatgccg
      661 gccagcttgc gcagggcctt ctccttgcgg cggcggtgct tgtagacgcc ggccgcctcc
      721 tcgatggccg cccggcggtc ctcgggccgg cccccgagca cgtcctcgag gcggttctgc
      781 ccgagcacgg tgtgcagctc gcggccgacg ccggtgtcgg acagcagctc ctggatgtcg
      841 agcagccggc aggtggtgcc gttgatggcg tagtcgctct cgccggagcg gtacagcagc
      901 cgggtgacgg tcacctcgct gtactcgatg ggcagcagcc cggccgagtt gtcgatggtg
      961 agggcgacct cggcccggcc gagggcgccg cggctgcccg tgccggcgaa gatgacgtcc
     1021 tccatcttgc cgccgcgcag cgacttgggg ccctgctcgc ccagcaccca ggcgacggcg
     1081 tcgaccacgt tggacttgcc ggaaccgttg gggccgacga tgaccgtgat ccccggctcg
     1141 aagtccaggg tggtcttctc ggcgaacgac ttgaacccgc gcagggtcag cgacttgagg
     1201 aacatcaggc cggctgatgc tagcacgggg atcggacgat cccggggatg tcggcgccga
     1261 tgggggtgaa cgttcggggc ctcccgcggg aggccccgaa atagacgaaa ccctggctag
     1321 gccagggccg cttccttggt ggtcgagggg cggaagtcct cgacctcgcg aagggcgagc
     1381 tcctgctccg ctaccgcgag gtgccgctcg agctcggcga cacgcgcttt gagggcattc
     1441 agctcccgca agatcatgta ggcgggcggc tgacctacgt agcccatgag cgccttcgac
     1501 atccgggaag ctcctcccac tgcagcggct tcggggttac cgctgcgctc ttgtccgctg
     1561 gactggcagg ccacaagggt cgtccagcgc cgtgcggaac ggccgacgac tacttacggt
     1621 ttgtaccctg ggagaggaag gcccaaaacg tgtgcgtgac cctgcccgat gcagccgggc
     1681 cgagcagtta ccgcgtccgg gcggtcccag aggactgatt tcacaagcgg actataacac
     1741 gtccgtatgg aggcgccaag cgagtccatc gcgacgaaac cgccgtccgg ttaccgcgcc
     1801 tcgaagtagt ggaactcgcc ggtggccggg atccgctggc tgtcgagcga ctcgacctcg
     1861 gcccagcgcg ggccctcgcg caggaacgac tcgagctggt cgagccggtc gggatcgccc
     1921 tcggcctcga cccggaccga cccgtcgggc aggttctcgg cgtagccggt gagcccgagc
     1981 tgcccggcct ggcgctgcac ggcccagcgg aagcccacgc cctggacctc gccccggatg
     2041 acggccttga gccggagcgc
//
LOCUS       DASSWR010000690         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1071949, whole genome shotgun sequence.
ACCESSION   DASSWR010000690 DASSWR010000000
VERSION     DASSWR010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071949"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFO47_11595"
     CDS             complement(<1..440)
                     /locus_tag="VFO47_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8789379.1"
                     /translation="MTAAPVASPRSWAGEPLEGHDPVPLPRFLLEHARDLGDKPALVD
                     GPTGRTLSYRQLAAGVERVAAGLAARGFGHGDVLAVSSPNLPEYALAVYGAMAAGGAV
                     TGANPLLTADELAGQLADSGASVLVTVPPFLQTAREAEARAGVG"
     gene            complement(437..646)
                     /locus_tag="VFO47_11600"
     CDS             complement(437..646)
                     /locus_tag="VFO47_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789380.1"
                     /translation="MDVIACPDPTCQAPAWVYDRWTWPSTDGPVEHVKTGCEADHHFT
                     PMAASLVPFRGPTPVTTRRPAEVVR"
     gene            772..>2138
                     /locus_tag="VFO47_11605"
     CDS             772..>2138
                     /locus_tag="VFO47_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8789381.1"
                     /translation="MEQGLRVRVLGATELAVDGRPVAGLASAKATALLVHLAVTGTAR
                     SRSALAGLLWSDLPEETARANLRLALTKLRRALPEQLLVTRQSVALDPARPVWVDAVE
                     VARLVAARAEGEELLAGARLCRGEFLAGFEVQGAELFDEWVLGRRAAARADQLALLER
                     AVEDARERRDTAAGVEVARRMLELEPLHEEAHRALMWFLATGGQRGAALAQFETCRYL
                     LREELGEEPSAAAVALRDQIAQAGGFADLGPAPAGAGAAAAGAAAAPRPLTSLIGREE
                     DLARLHALLDDPACRLVTLVGPGGIGKTRLALEAGATRRGRYRDGVLTVSFVGTSPAR
                     PEEAADLVVTNLAAALGVSLAVPRDPRELLADHLAGQELLLILDNLEQLRDAAGVLAE
                     LLARAPGVQLLGTSRRRLGLGVEWLVEVPGLPYPPPGADAAGYPAVQLFEDRARLLRP
                     GFRPAA"
BASE COUNT          214 a          774 c          893 g          257 t
ORIGIN      
        1 tccccgaccc cggcccttgc ctccgcctcc ctggccgtct ggaggaacgg cgggacggtc
       61 acgagcacgc tggcgcccga gtcggccagc tggccggcca gctcgtcggc ggtcagcagg
      121 ggattggcgc cggtgacggc gccgccggcc gccatcgccc cgtagaccgc cagggcgtac
      181 tcgggcaggt tggggctgga gacggcgagc acgtcgccgt gaccgaagcc gcgcgcggcc
      241 aggccggccg cgacccgctc gaccccggcg gccagctgcc ggtaggacag cgtccggccg
      301 gtcgggccgt cgaccagggc cggcttgtcg cccaggtccc gggcgtgctc gagcaggaag
      361 cgggggagcg ggaccgggtc gtggccctcg agcggctcgc cggcccagct gcgggggctg
      421 gccacgggcg cggcggtcat cggacgacct ccgccgggcg ccgggtggtg accggggtgg
      481 ggccgcggaa cgggaccagc gaggcggcca tcggggtgaa gtggtggtcg gcctcgcagc
      541 cggtcttgac gtgctcgacc gggccgtcgg tggacggcca ggtccagcgg tcgtacaccc
      601 aggccggggc ctggcaggtc gggtcggggc aggcgatgac gtccacgatg ggctcccctc
      661 ggagtcggtt acctggccgg acgctagccg gccggcgttt cccgaccgtt tgcccgtcgt
      721 gttcgcgccg ccgttccgat aaggtgcgga gaggagaggg caggggcgac gatggagcag
      781 gggcttcgcg tccgtgtgct cggggcgacc gagctggccg tggacgggcg cccggtggcc
      841 gggctggcgt cggccaaggc gacagcgctg ctcgtccacc tggcggtgac cgggacggcg
      901 cggtcgcgct cggccctggc cggcctgctc tggagcgacc ttcccgagga gaccgcccgg
      961 gccaacctgc gactggccct gaccaagctg cgccgggcgc tgccggagca gctgctggtg
     1021 accaggcagt cggtcgccct cgacccggcc cggccggtct gggtcgacgc ggtcgaggtg
     1081 gcgcggctgg tcgcggccag ggcggagggg gaggagctgc tggccggggc gcggctctgc
     1141 cgcggggagt tcctggccgg cttcgaggtc cagggggccg agctgttcga cgagtgggtg
     1201 ctcggccggc gggccgccgc ccgggccgac cagctggcgc tgctggagcg ggccgtcgag
     1261 gacgcccgcg agcgccgcga caccgccgcc ggggtcgagg tcgcccgccg catgctggag
     1321 ctggagccgc tgcacgagga ggcgcaccgg gcgctgatgt ggttcctggc caccggtggc
     1381 cagcgcggcg ccgccctggc ccagttcgag acctgccgct acctgctccg cgaggagctg
     1441 ggcgaggagc cgtcggcggc cgccgtcgcc ctgcgcgacc agatcgccca ggcgggcggg
     1501 ttcgccgacc tgggcccggc cccggccggg gccggagcgg ccgcggccgg ggcggccgcg
     1561 gcgccgcggc cgctgacctc gctgatcggg cgggaggagg acctggcccg gctgcatgcc
     1621 ctgctcgacg accccgcctg ccggctggtg accctggtcg ggccgggtgg catcggcaag
     1681 acccggctgg ccctggaggc cggcgcgacc cggcggggac gctaccgcga cggggtgctg
     1741 acggtgtcgt tcgtcgggac cagcccggcg cggccggagg aggcggctga cctggtggtg
     1801 accaacctgg ccgccgcgct gggcgtgtcc ctggccgtcc cccgcgaccc gcgggagctg
     1861 ctcgccgacc acctggccgg ccaggagctg ctgctgatcc tcgacaacct cgagcagctc
     1921 cgcgacgccg ccggggtgct ggccgagctg ctcgcccggg ccccgggggt gcagctgctg
     1981 ggcacgtccc ggcggcgcct ggggctcggg gtcgagtggc tggtcgaggt gcccggcctg
     2041 ccctacccgc cgcccggggc ggacgcggcc ggctacccgg ccgtgcagct gttcgaggac
     2101 cgggcccggc tgctccggcc cgggttccgc ccggccgc
//
LOCUS       DASSWR010000691         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_591900,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000691 DASSWR010000000
VERSION     DASSWR010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591900"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /gene="cofC"
                     /locus_tag="VFO47_11610"
     CDS             complement(<1..437)
                     /gene="cofC"
                     /locus_tag="VFO47_11610"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET8789382.1"
                     /translation="MKALAEAKGRLAPEVGPLQRRLLAIAMFEDVVAALQSVRGLERP
                     VVVSPDREVWRRADAMGCRVVEEPAGAGDLNGALAAAAATANGSGLLVVAADLPLASA
                     TGLERVLAAATAPVAVVPSHDGGGTNVLAWRDPASFAPSFGPAS"
     gene            487..1188
                     /locus_tag="VFO47_11615"
     CDS             487..1188
                     /locus_tag="VFO47_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8789383.1"
                     /translation="MAGLRYPVYTLAKVVVPPVIRFWVRLDCQGLLNIPREGPVIIAA
                     NHISYFDPLCLGTFVDTAGRQVRFLAKSELYRNPLLGAVLRGAGQIPVYRETRDAHLA
                     LTDAVAAMREGAAVAIYPEGTTTRNADFSPMAAKNGVARLAALTGAPVVPVGQWGAHL
                     LFTRGRLGPFRRGVRVVEKAGPPIDLGLEPDASLAEINLAKDKVMEAITGLVAEAREG
                     WSPPAWYRPKAAGQT"
     gene            1185..1658
                     /locus_tag="VFO47_11620"
     CDS             1185..1658
                     /locus_tag="VFO47_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789384.1"
                     /translation="MTERFQPGGVTHALVSGLVELALASALLAGSLAVSESSPGAATS
                     LSVASLLWAVLAVVSLVVAWLRARVLAAELDDEAVTLHGLGGARRYRYDELSAVEVSG
                     RRTRLVARDGGVRVVRGVRGPAQGNRFRARVLARAKAAVGVDSLPADQREGDSDG"
     gene            1651..>2498
                     /locus_tag="VFO47_11625"
     CDS             1651..>2498
                     /locus_tag="VFO47_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8789385.1"
                     /translation="MADGFATRSIHAGRLPLPAEPPLATPIWQTSTFVFDDAEHYAHT
                     LRQPREGYVYTRYENPTTAALEATVADLEGAAEGLATASGMGAIATVLLSLAGTGDHV
                     VAQRDLYGGTHSLLAHTAPRFGIEVSFVDAGDPEAVRAALRPTTRAIYAETVANPTMT
                     VADLPTLAEVGAAAGVPLVVDNTVATPYLCRPLEHGAAVVVHSATKYLGGHSDVVGGL
                     ALFAEPGRHDAAWRTMIELGASPDPFAAWLTLRGIKTLALRMRAHGANARLLAELLDA
                     HPKVTRV"
BASE COUNT          312 a          990 c          896 g          300 t
ORIGIN      
        1 gacgccgggc cgaagctggg cgcgaacgac gccgggtcgc gccaggcgag cacgttggtg
       61 ccgccgccgt cgtggctcgg gaccaccgcc accggcgccg tcgccgccgc cagcacccgc
      121 tccagcccgg tcgccgaggc cagcggcagg tcggccgcca ccaccagcag ccccgacccg
      181 ttggcggtgg cggccgccgc ggccagggcg ccgttcaggt cgccggcccc cgccggctcc
      241 tcgaccaccc ggcagcccat ggcatcggcc cgccgccaca cctcacggtc gggggagacc
      301 accaccggcc gctccagccc ccggaccgac tggagggccg cgaccacgtc ctcgaacatg
      361 gcgatggcca gcagccgccg ctgcaacggc ccgacctcgg gcgcgaggcg gcccttggcc
      421 tcggccaggg ccttcacggg aatcagcgga acaggcatcg ccgggcaacg gtactatgcg
      481 ccgagcatgg ccggcctccg gtatcccgtc tacaccctgg ccaaggtcgt cgtgccgccg
      541 gtgatccgct tctgggtccg gctcgactgc cagggtctgc tcaacatccc ccgcgaaggc
      601 ccggtgatca tcgccgccaa ccacatctcc tacttcgacc cgctgtgcct tgggaccttc
      661 gtcgacacgg ccgggcgcca ggtccgcttc ctggccaagt cggagctgta ccgcaacccg
      721 ctgctggggg cggtcctgcg cggggccggg cagatcccgg tgtaccgcga gacccgcgac
      781 gcccacctgg ccctgaccga cgccgtggcg gccatgcggg agggggcggc ggtggccatc
      841 tacccggagg gcaccaccac ccgcaacgcc gacttctccc cgatggcggc caagaacggg
      901 gtcgcgcgcc tggccgccct caccggggcg ccggtcgtgc cggtcggcca gtggggcgcc
      961 cacctgctgt tcacccgggg caggctgggc ccgttccggc ggggcgtccg ggtggtcgag
     1021 aaggccgggc cgccgatcga cctcgggctg gagcccgacg cctccctggc cgagatcaac
     1081 ctggccaagg acaaggtgat ggaggccatc accggcctgg tcgccgaggc ccgggagggc
     1141 tggagcccgc ccgcctggta ccggcccaag gcggccggcc agacatgacc gagcggttcc
     1201 agcccggcgg cgtgacccac gcgctggtca gcgggctggt cgagctggcc ctggcctcgg
     1261 ccctgctggc cggctcgctg gcggtgtcgg agtcgagccc gggggcggcc acctccctgt
     1321 cggtggccag cctgctctgg gccgtcctgg ccgtcgtcag cctggtcgtg gcctggctgc
     1381 gggcgcgggt gctggccgcc gagctggacg acgaggcggt gaccctgcac gggctgggcg
     1441 gggcccgccg ctaccgctac gacgaactgt cggccgtgga ggtgtcgggc cggcggaccc
     1501 ggctggtggc ccgcgacggc ggggtccggg tggtccgcgg ggtccgcggc cccgcccagg
     1561 gcaaccgctt ccgggcccgg gtcctggccc gcgccaaggc ggcggtggga gttgatagcc
     1621 tgcccgccga ccagagggaa ggggacagcg atggctgacg gcttcgccac ccgatcgatc
     1681 cacgccggcc gcctgcccct gcccgccgag ccgccgctgg ccaccccgat ctggcagacc
     1741 tcgaccttcg tcttcgacga cgccgagcac tacgcgcaca ccctccgcca gccgcgggag
     1801 ggctacgtct acacccgcta cgagaacccg accacggccg ccctggaggc gaccgtggcc
     1861 gacctggagg gcgcggccga gggcctggcc accgcctcgg gcatgggcgc catcgccacc
     1921 gtgctgctgt cgctggccgg gaccggcgac cacgtggtcg cccagcgcga cctgtacggc
     1981 gggacacact cgctgctggc ccacaccgct ccccgcttcg gcatcgaggt cagcttcgtc
     2041 gacgccggcg acccggaggc cgtccgggcc gccctgcgcc caacgaccag ggccatctac
     2101 gccgagacgg tcgccaaccc gaccatgacc gtggccgacc tgcccaccct ggccgaggta
     2161 ggcgccgccg caggcgtgcc cctggtggtc gacaacaccg tggccacgcc gtacctgtgc
     2221 cggcctctgg agcacggggc ggccgtggtc gtgcactcgg ccaccaagta cctgggcggc
     2281 cacagcgacg tggtcggcgg gctcgccctg ttcgccgagc cggggcggca cgacgccgcc
     2341 tggcggacca tgatcgagct cggcgcctcg cccgacccgt tcgccgcctg gctgaccctg
     2401 cgcgggatca agaccctggc cctgcgcatg cgcgcccacg gcgccaacgc ccggctgctg
     2461 gccgagctcc tggacgccca ccccaaggtg acccgcgt
//
LOCUS       DASSWR010000692         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1185179, whole genome shotgun sequence.
ACCESSION   DASSWR010000692 DASSWR010000000
VERSION     DASSWR010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185179"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFO47_11630"
     CDS             <1..580
                     /locus_tag="VFO47_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HET8789386.1"
                     /translation="CYAGDAEAGERALAPFRALAEPVADMLQPMPYPGLFEPAEDIEV
                     VEESARSMFLDRVDRAAAEAVVEHLQASTAPMAVAQLRVLGGAMARVPAEATAFAHRD
                     RRFMAAVGAVYEHAADRPAHEAWAEAFAAALRGDGGPGVYVNFLSDEGPERVREAYPG
                     PTWDRLAEIKRRYDPGNLFRRNQNIPPAPGPA"
     gene            689..1531
                     /locus_tag="VFO47_11635"
     CDS             689..1531
                     /locus_tag="VFO47_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET8789387.1"
                     /translation="MGNRPEAGVSELNPLAEVRQARMAGIIWDEGFARVTDLADRFGV
                     SAVTVRADLNALEARARVRRVRGGAVPAPGLLREPPPELPLGERPFESSQWEAPVQKS
                     SIGLHAAAMVSDGDTVILDVGTTTTSIARALVLRTDLHDVTVVTNGLNIALELERATP
                     RISVVVTGGTLRPLQHSLVNPLGTTLLERLHASVAFVGCNGVDPEVGITNINLPEAEV
                     KRAMLLAARRRVIVADGSKVGEVELAKVCDIEEVSLLITDPTADPEVVAEIAAAGCRV
                     EYAG"
     gene            1602..2945
                     /locus_tag="VFO47_11640"
     CDS             1602..2945
                     /locus_tag="VFO47_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8789388.1"
                     /translation="MSRPRSESEYLARLLPPSVAATGLSRRGFLKGTLGAGAAVSLSS
                     LLAACGGGDGGEGDSASKEVTFGSNQSDEVPKKAYAEMTSAFGQKSGMTVKTNTVEHE
                     TFQENINNYLQGNPDDVFTWFAGYRMQFFASQGLAGDISDVWGDISGMSEAMKAASTG
                     EDGKQYFVPLTTYPWGLFYRKSVWEEKGYQPPETLDELVTLAKQMEKDGLIPIAFADK
                     DGWPAMGTFDQLNLRINGYQFHVDLMAGKQAWDSPQVKKVFDTWRGLLPYHQPDALGR
                     EWQEAARALQKKQAGMYLLGMFVGEQFPEKDRDDLDFFNFPEIDPAIGTGAIEAPIDG
                     VMMRAEPKNEEGAKQFLRFTGTAEAENVLLKANNTVIGSSTEADSSNYTGLQKKAVEF
                     IGAAESIAQFLDRDTRPDFASTVMIPGLQTFIKNPNDIDGLTKSLESQKKSIFTS"
     gene            2961..>3159
                     /locus_tag="VFO47_11645"
     CDS             2961..>3159
                     /locus_tag="VFO47_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8789389.1"
                     /translation="MTDLPTVPVTPAEAAPRRPARPRRGRRVQTTGTDRLFLVLMVAV
                     PTALVVGLVWLPAVATVVLSFA"
BASE COUNT          498 a         1166 c         1081 g          414 t
ORIGIN      
        1 gtgctacgcg ggcgatgccg aggccggcga gcgggcgctg gcgccgttcc gggccctggc
       61 cgagccggtc gccgacatgc tccagccgat gccctacccg gggctgttcg agccggccga
      121 ggacatcgag gtcgtcgagg agtcggcccg ctcgatgttc ctggaccgcg tcgaccgggc
      181 cgccgccgag gcggtggtcg agcacctcca ggcctccacc gcccccatgg ccgtggccca
      241 gctgcgggtg ctgggcgggg ccatggcccg cgtcccggcc gaggccaccg cgttcgccca
      301 ccgcgaccgg cgcttcatgg ccgcggtcgg cgccgtctac gagcacgccg ccgaccggcc
      361 cgcccacgag gcgtgggcgg aggcgttcgc ggccgcgctg cgcggcgacg gtggcccggg
      421 ggtgtacgtc aacttcctca gcgacgaggg acccgagcgg gtccgcgagg cctacccggg
      481 ccccacctgg gaccggctgg ccgagatcaa gcgccgctac gacccgggca acctgttccg
      541 ccgcaaccag aacatcccgc cggcgccagg gcccgcctga cccggggccc gcgcttgcgg
      601 aatgcgaacg accgcgaaga cttgacatag ctcgcgcaag cgttcgcaat gtgcccctag
      661 gcggaccatg ccccacagga gggcagaggt gggcaaccgg ccagaggcgg gcgtctccga
      721 gctgaacccg ctcgcggagg tgcgccaggc gcgcatggcc gggatcatct gggacgaggg
      781 gttcgcgcgg gtcaccgacc tggccgaccg gttcggggtg tcggcggtca cggtccgggc
      841 cgacctgaac gcgctcgagg cccgggcccg ggtccgccgt gtgcgcgggg gggccgtccc
      901 ggccccgggg ctgctccgtg agccgccccc ggagctgccc ctgggggagc ggcccttcga
      961 gtcgtcgcag tgggaggcgc cggtccagaa gtccagcatc ggcctgcacg cggcggccat
     1021 ggtcagcgac ggggacacgg tcatcctgga cgtgggcacg accacgacgt ccatcgcccg
     1081 cgcgctcgtg ctccgcaccg acctgcacga cgtcaccgtg gtcaccaacg ggctcaacat
     1141 cgccctggag ctggaacggg ccaccccccg catcagcgtc gtcgtcaccg ggggcaccct
     1201 tcgccccctg cagcactcgc tggtcaaccc gctgggcacg accctgctgg agcgcctgca
     1261 cgcctcggtg gccttcgtcg gctgcaacgg cgtcgacccc gaggtcggca tcaccaacat
     1321 caacctgccc gaggccgagg tcaagcgggc catgctgctg gccgcccggc gccgcgtgat
     1381 cgtcgccgac gggtccaagg tgggcgaggt cgagctggcc aaggtgtgcg acatcgagga
     1441 ggtgagcctt ctgatcaccg accccaccgc cgaccccgag gtggtggccg agatcgccgc
     1501 cgccggctgc cgagtggagt acgccggcta ggcccgaacg accgcaccac cgtccgtcgg
     1561 tccaacgtcc acggcgcccg agcgccacgc taggaggcga catgtcccgt ccccggtcgg
     1621 aatccgagta cctggcccgc cttctgccgc cgtcggtggc ggcgactggc ctcagccgcc
     1681 gcggcttcct caagggcacc cttggcgccg gggcggccgt ctcgctctcc agcctgctcg
     1741 ccgcctgcgg cggcggcgac ggcggcgagg gcgacagcgc ctccaaggag gtcaccttcg
     1801 gctccaacca gtccgacgag gtgcccaaga aggcctacgc cgagatgacc tcggccttcg
     1861 ggcagaagtc gggcatgacg gtgaagacca acaccgtcga gcacgagacg ttccaggaga
     1921 acatcaacaa ctacctgcag ggcaaccccg acgacgtgtt cacctggttc gccggctacc
     1981 ggatgcagtt cttcgcctcc cagggcctgg ccggcgacat cagtgacgtc tggggcgaca
     2041 tctccggcat gagcgaggcg atgaaggccg cctccaccgg cgaggacggc aagcagtact
     2101 tcgtgcccct gaccacctac ccgtgggggc tcttctaccg caagtctgtc tgggaggaga
     2161 agggctacca gccccccgag accctggacg agctggtcac cctggccaag cagatggaga
     2221 aggacgggct gatcccgatc gccttcgccg acaaggacgg ctggccggcc atgggcacct
     2281 tcgaccagct caacctgcgc atcaacggct accagttcca cgtcgacctg atggccggca
     2341 agcaggcctg ggactcgccc caggtcaaga aggtgttcga cacctggcgg gggctgctgc
     2401 cctaccacca gcccgacgcc ctggggcgcg agtggcagga ggcggcccgg gccctgcaga
     2461 agaagcaggc ggggatgtac ctgctcggca tgttcgtggg cgagcagttc cccgagaagg
     2521 accgcgacga cctcgacttc ttcaacttcc ccgagatcga cccggccatc ggcactgggg
     2581 cgatagaggc cccgatcgac ggcgtcatga tgcgcgccga gcccaagaac gaggagggcg
     2641 ccaagcagtt cctcaggttc accggcaccg ccgaggccga gaacgtcctc ctcaaggcca
     2701 acaacaccgt catcgggtcc agcaccgagg ccgattccag caactacacc gggctgcaga
     2761 agaaggcggt cgagttcatc ggtgcggccg agtcgatcgc ccagttcctg gaccgcgaca
     2821 cccggcccga cttcgcctcg acggtcatga tccccggcct gcagaccttc atcaagaacc
     2881 cgaacgacat cgacgggctg accaagagcc tggagagcca gaagaagtcg atcttcacct
     2941 cctgagacgg ggaggcgccg atgaccgacc tgcccactgt cccggtcaca cccgccgaag
     3001 cggccccgcg ccgcccggcc cggccccggc gcggccgccg cgtccagacg accggcaccg
     3061 accgactctt cctggtcctc atggtggccg tccccacggc cctggtggtg ggactggtct
     3121 ggctgcccgc ggtcgccacc gtggtgctgt cgttcgcca
//
LOCUS       DASSWR010000693         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1298411, whole genome shotgun sequence.
ACCESSION   DASSWR010000693 DASSWR010000000
VERSION     DASSWR010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298411"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFO47_11650"
     CDS             complement(<1..192)
                     /locus_tag="VFO47_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789390.1"
                     /translation="MTVIEMDGAGKHLAPLGCAAVDADSPPASAPRRSRWRTVAVLAV
                     VAGFFVATLAARWNDVISLK"
     gene            237..776
                     /locus_tag="VFO47_11655"
     CDS             237..776
                     /locus_tag="VFO47_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789391.1"
                     /translation="MAVKVVHVSDISGKEADEQSLGRLVVHEHPEYADLPVTLEVLPE
                     EVETLQGASRFVTVEWIAPGARKGERMVVSLDDFNLLAGAGVMESAVQDALIAKHRNR
                     PRSVAGGGSGAGGRSGRGKVNYATLEHAGEPHRGRITEAEKELVRKNLDQINRRLREA
                     GQREIDPSDQAMRDRYGLE"
     gene            870..1571
                     /locus_tag="VFO47_11660"
     CDS             870..1571
                     /locus_tag="VFO47_11660"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HET8789392.1"
                     /translation="MARLRVLVGAILTAALVLAFAAPASATGRGTTKVRAFHNSPDTP
                     AVDIYVNGAKTLSDVTYGTLSDYLEVPKGTYNVQVKVAPSTGSDPAALEADVRLGRRP
                     TTIAAIGSLTGDGGALQLKVLRDRDTVSRHLTRLRVAHTSPDAPAVDVQAKLAGRWIR
                     VVRNLEFGEATGYLLLPAWKYRLRIVAAGTNTVVKDLGKVRLKGSTSYTAWAVGFLSP
                     TDDYPGFDVFVSVDG"
     gene            1582..2034
                     /locus_tag="VFO47_11665"
     CDS             1582..2034
                     /locus_tag="VFO47_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET8789393.1"
                     /translation="MESVWDYPRPPRVEPLAARVVVELDGEVLADTRRSLRVLETSHP
                     PVYYIPAADIRTDRLRPSARRPTVCEFKGTARYYDAGDREAVAWSYASPSPGYEAIAG
                     CYAFYPGRVDRATVDGEQVEAQPGDFYGGWITSTVTGPFKGGPGTTGW"
     gene            2056..2829
                     /locus_tag="VFO47_11670"
     CDS             2056..2829
                     /locus_tag="VFO47_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET8789394.1"
                     /translation="MPVLVGTSGWQYRDWRGRLYPQGLALARWLEHYAERFATVESNN
                     AFYRLPEPGTFAAWAERTPPDFVMAVKASRFLTHLKRLRDPEEPVGRLMDHARHLGGK
                     LGPVLLQLPPTLKADPAALDRTLRAFPAGTRVAVEPRHDSWWTDEVRDLLAGHGAALC
                     LADSPRRPTPVWRTADWTYLRLHEGTADPRPCYDRPALAAWAARLAELVGPGADAYVY
                     FNNDPGGCAVRDAVWFAGEAARAGLHPTRVPAPDEAPAG"
BASE COUNT          403 a         1104 c          965 g          370 t
ORIGIN      
        1 tttcagggaa atgacgtcat tccagcgcgc ggccagggtg gccacgaaaa agcccgcgac
       61 cacggccagc accgcgaccg tgcgccagcg ggagcgcctc ggagcgctcg caggcggcga
      121 atcggcatcc acagccgcgc atcctagcgg cgccaggtgt ttgcctgccc cgtccatttc
      181 gattaccgtc ataggcccgg cagacctttt tccgtttcga ggaggaatcc cccctgatgg
      241 cagtcaaggt cgtccatgtg tcggacattt ccggcaagga ggcggacgag cagtcgcttg
      301 gtcggctcgt ggtccatgag caccccgagt acgccgacct gccggtgacc ctggaggtcc
      361 tccccgagga ggtcgagacg ctccagggcg ccagccgctt cgtcaccgtc gagtggatcg
      421 cccccggcgc ccgcaagggc gagcggatgg tggtcagcct cgacgacttc aacctgctgg
      481 caggggcggg cgtgatggag tcggccgtcc aggacgccct gatcgccaag caccgcaacc
      541 gcccccgctc ggtcgccggc ggcgggtcgg gggcgggcgg ccgctcgggc cgtggcaagg
      601 tcaactacgc cacccttgag cacgccgggg agccccaccg gggccgcatc accgaggccg
      661 agaaggagct ggtccgcaag aacctggacc agatcaaccg gcgcctccgc gaggccggcc
      721 agcgcgagat cgaccccagc gaccaggcga tgcgcgaccg ctacggcctc gagtagcacc
      781 gctccagctt cgcgattccc tgcaaccggt cgccgggccg gccgcgaaat acaggtcgtg
      841 cccagttcct cgttgcagga ggtcatgtag tggcaagact ccgcgtcctg gtcggcgcga
      901 tcctgaccgc cgccctggtc ctggccttcg cggccccggc ctcggccacc ggccgcggga
      961 ccaccaaggt ccgcgccttc cacaacagcc ccgacacgcc ggcggtcgac atctacgtca
     1021 acggcgccaa gacgctgtcg gacgtcacct acggcaccct cagcgactac ctcgaggtgc
     1081 ccaaggggac ctacaacgtg caggtcaagg tggccccgtc gaccgggagc gaccccgccg
     1141 ccctcgaggc cgacgtgcgc ctcggccgcc gcccgaccac catcgccgcc atcggctcgc
     1201 tgaccggcga cggcggcgcc ctccagctca aggtgctgcg cgaccgcgac accgtctccc
     1261 gccacctcac gcggctgcgg gtggcccaca ccagccccga cgccccggcc gtcgacgtcc
     1321 aggccaagct ggccggccgc tggatccggg tcgtccggaa cctcgagttc ggcgaggcca
     1381 ccggctacct gctgctgccg gcctggaagt accggctgcg gatcgtcgcc gccggcacga
     1441 acaccgtggt caaggacctc ggcaaggtcc ggctcaaggg cagcacctcc tacaccgcct
     1501 gggcggtggg cttcctcagc cctaccgacg actaccctgg cttcgacgtg ttcgtctccg
     1561 tggacggctg atcggaagag catggagtcc gtctgggact acccgcggcc gccgagggtc
     1621 gagcccttgg cggcccgcgt ggtcgtcgag ctggacgggg aggtgctggc cgacacccgc
     1681 cgcagcctgc gggtgctgga gaccagccac ccgcccgtct actacatccc cgccgccgac
     1741 atccgcaccg accggctgcg tccctcggcc aggcggccca cagtctgcga gttcaagggc
     1801 acggccaggt actacgatgc aggcgaccgc gaggcggtcg cctggtccta tgcgtcgccc
     1861 agccccgggt acgaggccat cgccggctgc tacgccttct acccgggccg ggtggaccgg
     1921 gcgaccgtcg acggcgagca ggtcgaggcc cagccgggcg acttctacgg cggctggatc
     1981 acctcgacgg tgaccggccc gttcaagggc gggccgggga cgacgggctg gtgacgggac
     2041 ggaggtgagc ggctggtgcc ggtgctggtc gggacctctg gctggcagta ccgggattgg
     2101 cggggccgcc tctacccgca ggggctggcc ctggcccgct ggctggagca ctacgccgag
     2161 cggttcgcca ccgtcgagtc caacaacgcc ttctaccggc tgcccgagcc gggcaccttc
     2221 gccgcctggg ccgagcggac cccgcccgac ttcgtgatgg ccgtcaaggc cagccgcttc
     2281 ctgacccacc tcaagcgcct gcgtgacccc gaggagccgg tcggccgcct gatggaccac
     2341 gcccgccacc tgggcggcaa actcggcccg gtgctgctcc agctcccccc gaccctcaag
     2401 gccgacccgg ccgccctgga ccggaccctg cgggcgttcc cggccgggac ccgggtcgcg
     2461 gtcgagcccc gccacgacag ctggtggacc gacgaggtcc gcgacctgct ggccggccac
     2521 ggggccgccc tctgcctggc cgacagcccg cgccgcccca cgcccgtgtg gcgcaccgcc
     2581 gactggacct acctgcgcct ccacgagggc accgccgatc cccgcccctg ctacgaccgc
     2641 ccggccctgg ccgcctgggc ggcccgcctg gccgagctgg tcggtcccgg cgccgacgcc
     2701 tacgtctact tcaacaacga ccccggtggc tgcgccgtcc gcgacgccgt ctggttcgcc
     2761 ggggaggcgg cccgggccgg cctccacccg acccgggtcc cggcccccga cgaggccccg
     2821 gcgggctagc ggcggcgcag gt
//
LOCUS       DASSWR010000694         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_139897,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000694 DASSWR010000000
VERSION     DASSWR010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139897"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..464
                     /locus_tag="VFO47_11675"
     CDS             300..464
                     /locus_tag="VFO47_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789395.1"
                     /translation="MLAYLDAGSASLVIQAIVAGAAGAAVFFKLGWRRITSPFRRHTA
                     TAEVGEHDER"
     gene            503..1912
                     /locus_tag="VFO47_11680"
     CDS             503..1912
                     /locus_tag="VFO47_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8789396.1"
                     /translation="MSDLELEPASFRDPASTVAYLDGRVLRGLTKEGAADFDTLAAAP
                     FFAKAMDEGKLVRTTQVPIEDLPQSVRGDAWVRALEHERIPFVSYPYEWTFSMLRDAA
                     LVHLDLLLAALDADLTMKDGYAYNLAFVGSRPTFIDVGSFERVRPGEPWAGYRQFCQT
                     MLYPLLLQAHKDVRFQPWLRGAVQGIQPQDLAKLFSGTDKMKPGVLKHVSLHGAMDQR
                     YSRSRAQDTQKELKAAGFSTELQKATVAAIRKLVAKLEWSRSASEWNDYQRTSTYTDA
                     EREIKAAFVAKALEGKGARLVFDLGGNDGTYSRVAAGHADYVVCADGDDLVLDGLYRS
                     LRQEGNHQILPAYLDLSDPSPGLGWRGRERAGFFDRSRPDAVLALALLHHLAITGNVP
                     LPELVDWLHSLGGRLVVEFVDPTDPMADRLLANKPPGMHGDYRREVFERLLEARFDIA
                     DRQEFPSGTRTLYHATPRR"
     gene            1937..2013
                     /locus_tag="VFO47_11685"
     tRNA            1937..2013
                     /locus_tag="VFO47_11685"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1971..1973,aa:Ile,seq:cat)
BASE COUNT          313 a          783 c          690 g          305 t
ORIGIN      
        1 gggaccaggc gcccggcggc ggagtgcccg gtgggggagc ccacgcgggg ggctcggccg
       61 gcgtccgtgg ccagggctgc tggcccggtg gtgtcggcgg atcggtcggg tcggccacga
      121 ggctccttgc ggacgtcggg aatccccagc gtagcgcagg cggcgcagtt caccgttgtg
      181 taacggctgg ctgagggtcc ggccagcagc cgcccactcg tgatatcgtg ccgtccgatc
      241 tgccccaaac ccacgtcgtc cggccagttg tgacgggcga cgggaaggag acgcgttcta
      301 tgctcgccta cctcgatgcc ggatcggcca gcctggtgat ccaggcgatc gtggccggcg
      361 ccgccggtgc ggccgtgttc ttcaagctgg gctggcgcag gatcacctcc ccgttccgcc
      421 gccataccgc caccgccgag gtcggcgagc acgacgagcg ctgagcggcg ccccttcata
      481 gagcacaaac ggtaggaact ggatgagcga tctggagctg gagcctgcgt cgttccggga
      541 cccggcgagc acggtggcgt acctcgacgg ccgggtcctg cgcggcctga ccaaggaagg
      601 cgccgccgac ttcgacaccc tcgccgccgc cccgttcttc gccaaggcca tggacgaggg
      661 caagctggtc aggaccaccc aggttcccat cgaggacctg ccccagagcg tccgcggcga
      721 cgcctgggtg cgggcgctgg agcacgagcg gatcccgttc gtcagctacc cgtacgagtg
      781 gaccttctcc atgctccgcg acgccgccct ggtccacctg gacctgctgc tggccgccct
      841 cgacgccgac ctgaccatga aggacggcta cgcctacaac ctcgccttcg tgggctcgcg
      901 gccgaccttc atcgacgtcg gctcgttcga gcgggtccgg cccggtgagc cgtgggccgg
      961 ctaccggcag ttctgccaga cgatgctgta tccgctgctg ctccaggccc acaaggacgt
     1021 ccgcttccag ccgtggctgc gcggcgccgt ccagggcatc cagccccagg acctggccaa
     1081 gctgttcagc ggcaccgaca agatgaagcc gggcgtgctc aagcacgtct cgctccacgg
     1141 ggccatggac cagcgctact ccaggtcccg ggcccaggac acccagaagg agctgaaggc
     1201 ggccgggttc tcgaccgagc tccagaaggc caccgtggcc gccatccgca agctggtggc
     1261 caagctggag tggagccgct cggcctcgga gtggaacgac taccagcgga ccagcaccta
     1321 caccgacgcc gagcgcgaga tcaaggccgc cttcgtcgcc aaggcccttg agggcaaggg
     1381 agccaggctg gtcttcgacc tcggcggcaa cgacggcacc tactcgcggg tggccgccgg
     1441 ccacgccgac tacgtggtct gcgccgacgg cgacgacctg gtcctcgacg gcctgtaccg
     1501 ctccctgcgc caggagggca accaccagat cctgcccgcc tacctggacc tgtccgaccc
     1561 ctcgcccggc ctcggctggc ggggccgcga gcgcgccggc ttcttcgacc gctcgcgccc
     1621 cgacgccgtc ctcgccctgg ccctgctcca ccacctggcc atcaccggca acgtcccgct
     1681 tcccgagctg gtcgactggc tgcacagcct cggcggccgc ctggtggtcg agttcgtcga
     1741 ccccaccgac cccatggccg accgcctcct ggccaacaag cccccgggga tgcacggcga
     1801 ctaccgccgc gaggtgttcg agcgtctcct cgaggcccgc ttcgacatcg ccgaccgcca
     1861 ggagttcccc tccggcaccc gcaccctcta tcacgccacc ccgcgccgct agcccccgcg
     1921 gtacgatccg ggcaaagggg ctgtagctca gtcggtcaga gcaggcggct cataaccgct
     1981 cggtcgcagg ttcgaggcct gccagcccca ccaagcaagg caagcaagcc tagcacggct
     2041 tgccgagggg ctggcatccc tggtcattcc gtggtcattc cacttggttg a
//
LOCUS       DASSWR010000695         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1156260, whole genome shotgun sequence.
ACCESSION   DASSWR010000695 DASSWR010000000
VERSION     DASSWR010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156260"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2008
                     /locus_tag="VFO47_11690"
     CDS             <1..>2008
                     /locus_tag="VFO47_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8789397.1"
                     /translation="AGEKRLLAPGLAWRTGLIHYMRSELEQALEAFRRARLDGPLDAD
                     RALLLAWTATVHFLWNEVDACREAAGRALAAAAACGDDHALAAAHTAMAMLAALEGDR
                     AANDAHYLRALQAAERAGDVLHLARIRANLGSRSTEEGFYREALVELEAAMRLAELSG
                     YAAILALAQTNRGEAQLGLGRLDEAIAELEAAKVTYQRMGSHKVAYPLGDLGLVYRER
                     GDLALAKAAYEEAVGFAEQSGDLQGLVPALAGLARVLAGDEPNRAAELADRALGYGPG
                     MAWVEAQLAAGWVALAAGERERASAAAAAAAAAAGTRRDRAGLAEALELRALAAPDPA
                     GELPRLREAASIWAAIGNRLGEARNALAQARLAGSAAGDWGTSSGFPGGPGGVRGVRS
                     PAVEQTERRLRALGVRVGAEVAAGLLRAVAAPPRDPVRIQALGGFRVVRAGEPVPVQE
                     WQSRKARELLKVLVAQRGHAVPREALMEALWPDEDPARLSNRLSVALSTVRTVLDPER
                     RYASDRFVQADKHVVGLAHVPIDVEEFLTAAAAGLDRFTSGERAEALWLLATAETTYT
                     GDFLEDDPYEDWAVSLREEARAVYLAVARTLARSAAAAGEHDLAVRYHLRVLERDAYD
                     EEAHLGLVATLAAAGRHGEARRRYRIYGQKMDELGVEPTPFPSAS"
BASE COUNT          242 a          714 c          795 g          257 t
ORIGIN      
        1 gccggcgaaa agcggttgct ggccccgggg ctggcgtggc gcaccggcct gatccactac
       61 atgcgctccg agctcgagca ggccctggag gccttccgtc gggcgcgctt ggacggcccg
      121 ctcgacgccg accgggcgct gctgctggcc tggacggcca cggttcattt cctgtggaac
      181 gaggtcgacg cctgccgcga ggcggcaggg cgggcgctgg ccgcggcagc ggcctgcggc
      241 gacgaccatg cgcttgccgc cgcccacacc gccatggcga tgctggcggc gctggagggc
      301 gaccgggccg ccaacgacgc ccactacctg cgtgcgctgc aggcggctga gcgggccggc
      361 gacgtcctgc acctggcccg catccgcgcc aacctcggct cgcgcagcac cgaggagggt
      421 ttctaccggg aggcgctggt cgagctggag gctgcgatgc gactggccga gctgtccggc
      481 tacgccgcca tcctggccct ggcgcagacc aaccggggcg aggcccagct tgggctcggc
      541 cgcctcgacg aggccatcgc cgagttggag gcggccaagg tgacctacca gcggatgggc
      601 agccacaagg tcgcctatcc gctcggcgat ctcggcctgg tgtaccgcga gcggggcgac
      661 ctggccctgg cgaaggccgc ctacgaggag gccgtcggct tcgctgagca atccggcgac
      721 ctccagggcc tggtgcccgc cctggcgggc cttgccaggg tgctagccgg cgacgagccg
      781 aaccgggccg ccgagctggc cgaccgggcg ctcggctacg ggccgggcat ggcgtgggtt
      841 gaggctcagc tcgccgccgg ctgggtggcg ctggctgccg gcgagcggga gcgggcgtcg
      901 gccgccgcgg ccgccgcggc cgctgccgcc ggcacccgtc gcgatcgagc cggcttggcc
      961 gaggcgttgg agctgcgcgc cctggccgca cccgacccag ccggggaact gccgcggctg
     1021 cgggaggcgg cgtcgatctg ggcggccatc ggcaaccggc tcggcgaggc gcgcaacgcg
     1081 ctcgcgcagg cccgcctcgc cgggtcggcc gccggggact ggggaacctc ctcggggttc
     1141 cccggtggac cgggaggggt tcggggggtc cgctcccccg ccgtcgagca gaccgagcgg
     1201 cgcctgcggg cgctcggcgt gcgcgtcggc gccgaggtcg cggccgggct gctgcgggcg
     1261 gtcgctgccc cgccgcgcga cccggtgcgc atccaggcgc tcggcggctt ccgggtggtg
     1321 cgggcgggcg agccggtacc ggtgcaggag tggcagtccc gcaaggcccg cgagctgcta
     1381 aaggtcttgg tcgctcagcg agggcatgct gtgccgaggg aggcgctgat ggaggctctg
     1441 tggccagatg aggacccggc ccggctctcc aaccggctct cggtcgccct ctcgaccgtg
     1501 cgcaccgtgc tcgacccgga gcgacgctac gcctccgacc gcttcgtcca ggccgacaag
     1561 cacgtcgtcg gcctggccca cgtgcccatc gacgtggagg agttcctgac cgcggcggcg
     1621 gccggactgg atcggttcac ctcgggcgag cgagccgagg cgctgtggtt gctggctacg
     1681 gcggagacca cgtacaccgg cgacttcctg gaggacgacc cttacgagga ctgggcagtg
     1741 tcgctgcggg aggaggcaag ggcggtctac ctggcggtcg cgaggaccct ggcgcggtcg
     1801 gccgccgcgg cgggcgagca cgatctggcc gtccgctacc acctcagggt gctggagcgc
     1861 gacgcctacg acgaggaggc ccacctgggg ctggtggcca cgctggcggc ggccggccgc
     1921 cacggcgagg cgcggcgtcg ctaccggatc tacggccaga aaatggatga gctgggcgtc
     1981 gagccaacgc cattcccgtc ggcgagtc
//
LOCUS       DASSWR010000696         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1015528, whole genome shotgun sequence.
ACCESSION   DASSWR010000696 DASSWR010000000
VERSION     DASSWR010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015528"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFO47_11695"
     CDS             complement(<1..603)
                     /locus_tag="VFO47_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8789398.1"
                     /translation="MANPVVHFEIGAADDQRLLRFYGELFGWGLQAFPGVNYTLVDTR
                     GGGGINGGIGRSGTGEAWVSFYVEVDDPQAYLDKAEALGGRTVLEVIELPGNATFAMF
                     DDPDGLLVGLVHGEDAPQAPRRSSEGDGAAVDWFEVLGADAGRSQAFYAELFGWTVPP
                     GAYGQVAAGAEHGIGGGVGAGGTARWATVYAAVGDVEATLA"
     gene            complement(820..1977)
                     /locus_tag="VFO47_11700"
     CDS             complement(820..1977)
                     /locus_tag="VFO47_11700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8789399.1"
                     /translation="MDFRETAEQTMLREAVAKIAADYGHDYFLAKAKSGEKTTELWRA
                     VSEAGFVGVNLPEEYGGGGMGISELAIVTEELAAQGCPLMLLMVSPAICGTLLARFGT
                     PSQRDRWLPSIASGERKMVFAITEPDAGSNSHRLDTAATRDGDGWRLSGRKYYISGVD
                     EADAVLVVSRTGVEEGSGRGRLSLFIVDTDAPGLERTPIELHVNAPERQFFLFLDDVR
                     VGGDRLLGDEGDGLRQVFHGLNPERIIGAAAGNGIARYALAKAAAYAGQRQVWGVPIG
                     AHQGLAHPLAEAKIEVELARLMTQKAAWATDAGVPGAGEAANMAKYAAAEAALHAVDV
                     AIQVHGGNGFAAEYGIAGLWWMARVLRTAPVSREMILNYVAEHSLRLPRSY"
     gene            complement(1980..>2452)
                     /locus_tag="VFO47_11705"
     CDS             complement(1980..>2452)
                     /locus_tag="VFO47_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HET8789400.1"
                     /translation="SGDPAVHSPPNHAAATPAAPTTPTPTTSTPPAATPTDPTHRPTI
                     RAPLGRVFGARSGDKGGTANVGVWARTNEGYRFLEGFLTVDRFRELIPEARDLDVRRY
                     ELPNLLALNFVVAGLLGEGVSSSTRIDPQAKGLGEWLRARVVDLPAELLEPQGG"
BASE COUNT          312 a          943 c          848 g          349 t
ORIGIN      
        1 ggccagggtg gcctcgacgt ctccgacggc cgcgtacacc gtcgcccagc gggccgtccc
       61 gccggccccg acgccgcctc cgatgccgtg ctcggccccg gccgccacct gcccgtaggc
      121 gcccggcggc accgtccacc cgaacagctc ggcgtagaac gcctgcgacc gcccggcgtc
      181 ggcccccagc acctcgaacc agtcgaccgc cgccccgtcc ccctccgatg accgccgggg
      241 cgcctggggc gcgtcctcgc cgtgcaccag cccgaccagc agcccgtcgg ggtcgtcgaa
      301 catcgcgaag gtcgcattcc cgggcagctc gatgacctcc agcaccgtcc tccccccgag
      361 cgcctcggcc ttgtcgaggt acgcctgcgg gtcgtccacc tccacgtaga aggacaccca
      421 cgcctccccg gtgccgctcc tcccgatccc tccgttgatc ccgcccccac cccgcgtgtc
      481 caccagcgtg tagttcaccc cgggaaacgc ctgcagcccc cacccgaaca gctccccgta
      541 gaaccgcagc agccgctggt cgtcggccgc cccgatctcg aagtgcacga cagggttcgc
      601 catccccact cctctctgtt ccggggtgat tctctcagga gtccggacac cggcccgccc
      661 gccatccttc cattcgtccg ggtcgagggc cgccgggcgg tcctggcccg cacggccgac
      721 ctcctccctg atggccgagg ccgagacctc cggcgtgaac gccgttgcct ccttcgaccc
      781 ggcgatcgag cgggtcccca ccgtccgccg ggtcgagccc taataggacc gtggtaggcg
      841 cagggagtgc tcggccacgt agttgaggat catctcgcgg ctgaccgggg cggtgcgcag
      901 gacccgggcc atccaccaca ggccggcgat gccgtactcg gcggcgaagc cgttgccgcc
      961 gtggacctgg atggccacgt cgacggcgtg cagggccgcc tcggcggcgg cgtacttggc
     1021 catgttggcg gcctcgccgg cgccgggcac gccggcgtcg gtcgcccagg ccgccttctg
     1081 ggtcatcagg cgggccagct cgacctcgat cttggcctcg gccagggggt gggcgaggcc
     1141 ctggtgggcg ccgatgggca cgccccagac ctggcgctgt ccggcgtagg cggccgcctt
     1201 ggccagggcg tagcgggcga tgccgttgcc ggcggcggcc ccgatgatcc gctcggggtt
     1261 gaggccgtgg aagacctggc gcagcccgtc gccctcgtcc ccgaggagcc ggtcgccgcc
     1321 gacccgcacg tcgtccagga acaggaagaa ctgccgctcg ggggcgttga cgtggagctc
     1381 gatcggggtc cgttccagac cgggggcgtc ggtgtcgacg atgaacagcg acaggcgccc
     1441 ccggccgctg ccctcctcga ccccggtgcg gctgaccacc aggacggcgt cggcctcgtc
     1501 gaccccggaa atgtagtact tgcggccgct gagccgccag ccgtcgccgt cccgggtcgc
     1561 cgccgtgtcg agccggtggg agttcgaacc ggcgtcgggc tcggtgatcg cgaacaccat
     1621 cttgcgttcg cccgaggcga tcgacgggag ccagcgatcg cgctgggagg gcgtgccgaa
     1681 cctggccagg agggtgccgc agatggccgg cgacaccatc agcagcatca gcgggcagcc
     1741 ctgggcggcc agctcctcgg tcacgatggc cagctcgctg atgcccatgc ctccgccgcc
     1801 gtactcctcg ggcaggttga cgccgacgaa cccggcctcg ctcaccgccc gccacagctc
     1861 ggtcgtcttc tcccccgact tggccttggc caggaagtag tcgtgcccgt agtcggcggc
     1921 gatcttggca acggcctcgc gcagcatcgt ctgctcggcc gtctcgcgga agtccatcgt
     1981 caacctcctt gtggttccag cagctcggcg ggcaggtcga cgacccgcgc ccgcagccac
     2041 tccccgaggc ccttggcctg cgggtcgatc cgggtcgagg acgagacccc ctcgccgagg
     2101 aggccggcga ccacgaagtt gagcgcgagc aggttgggca gctcgtaccg ccgcacgtcg
     2161 aggtcgcgcg cctcgggaat cagctcccgg aaccggtcca cggtcaggaa cccctccagg
     2221 aaccggtagc cctcgttggt cctcgcccac accccgacat tggccgttcc ccccttgtcc
     2281 cccgaccgcg ccccgaacac ccgccccagc ggggcccgga tcgtgggccg gtgggtcggg
     2341 tcggtggggg tcgcggcagg gggcgttgag gtggtgggcg ttggggtggt gggcgcggcg
     2401 ggggtcgcgg cggcgtggtt aggggggctg tggacagccg gatcccccga ga
//
LOCUS       DASSWR010000697         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_930196,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000697 DASSWR010000000
VERSION     DASSWR010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930196"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2094
                     /locus_tag="VFO47_11710"
     CDS             <1..2094
                     /locus_tag="VFO47_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET8789401.1"
                     /translation="VEAIVERLEPKQELFARVEKTAGPGTVVTSNTSGLPLASLAEGR
                     SDDFRRRFCGTHFFNPPRYLKLVELIPTADTDPEVLERMRAFVERVLGKGAVVAKDTP
                     NFIANRLGSFAGMHDVRYALEHGYAIEEVDALTGPLVGRPKTATFRLFDQVGLDVMVA
                     VAGNLHELAPEDESRDVLPAPEPLVRMQEAGLLGNKTGGGFYRRGRRDGQTVFDVLDL
                     DTLKYRPAAKPDLPIVPAARDHDDPGTRLRFLLDKAEEDRGARYLRDTLLPSLAYAAR
                     RVPEIADSLVEVDHAVEWGFGYELGPFRAWDAIGVADGVGRMEALGIEVPAWVTEMLA
                     AGNDAFYRDGQVYSPITRAYEPVPADPEVLDLEARKAGGAVVAQNGSASLVDLGDGVL
                     CFELHAPASAIDAAVVELGARALQELEEGDWAGMVIANQARNFCVGANLGEVGMAAYQ
                     GLYDQVGEAVKGLQDVLMGFRHAARPVVAAPHGQTLGGGAEIVLHADRVVAALETYLG
                     LVEVGVGIVPAGGGCKELLRRVVAPAVRAAPDAPVLPFVQRVFETIGLAKVATSAVEA
                     QELGFLEEHDVIVLNADHQLAAAKQEVLDLADAYRPPAREASIYAAGLPVLAALELGV
                     QTLQWAGQASEHDGVVARHLARVLCGGELSLGQWVTEQHILDLEMEAFLALLREPKTM
                     ERIQGFLMTRKVVRN"
     gene            2107..3291
                     /locus_tag="VFO47_11715"
     CDS             2107..3291
                     /locus_tag="VFO47_11715"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET8789402.1"
                     /translation="MREAVIVSGARTAVGRAPRGSLRASRPDDMAGAAIAEALRRAEG
                     LDPAEVEDVLLGCAITEGSQGLNVARIAAQRAGLPETVPAQTVNRFCSSGLQTIASAA
                     ERIMAGFATTMVAGGTEHMSSTPTPTPTFAPNPEVVRGRPEYYMAMGLTGERVAERWG
                     VSREDQDAFALRSHQRAAEAVDSGRFDPEIVPLPVRVDTLDGDGRAVTSEGTFSRDEG
                     PRRDTSAEALAKLKPVFKQGGTVTAGNSSQRSDGAAAVVVMERERAERLGLAPLGRFV
                     GYAVGGVAPEVMGIGPTVAIPKVLELTGLRLDDIDLIELNEAFASQTLAVIREVGLDL
                     DKVNVNGGAIALGHPMGATGTKLTVQLLAELARRGGRYGMVTMCVGGGQGAAGIFENL
                     QR"
BASE COUNT          496 a         1414 c         1388 g          455 t
ORIGIN      
        1 gtcgaggcga tcgtggagcg cctggagccc aagcaggagc tgttcgcccg ggtcgagaag
       61 acggccggcc ccgggacggt ggtcacctcc aacacctccg ggctgccgct ggccagcctg
      121 gccgaggggc gctcggacga cttccggcgc cgcttctgcg gcacccactt cttcaacccg
      181 ccccgctacc tcaagctggt cgagctgatc ccgaccgccg acaccgaccc cgaggtgctg
      241 gagcggatgc gggcgttcgt ggagcgggtc ctcggcaagg gcgcggtcgt ggccaaggac
      301 acccccaact tcatcgccaa ccgcctgggc agcttcgccg gcatgcacga cgtccgctac
      361 gccctcgagc acggctacgc catcgaggag gtcgacgccc tcaccgggcc gctcgtgggc
      421 cggcccaaga cggccacctt ccggctgttc gaccaggtcg gcctggacgt gatggtcgcc
      481 gtggccggca acctgcacga gctggcgccc gaggacgaga gccgcgacgt gctcccagcc
      541 cctgagccgc tggttcggat gcaggaggcg ggcctgctcg gcaacaagac cggcggcggc
      601 ttctaccggc gcggcaggcg cgacggccag accgtcttcg acgtgctcga cctggacacc
      661 ctgaagtacc ggccggcggc caagccggac ctgccgatcg tccccgcggc ccgcgaccac
      721 gacgacccgg gcacgcgcct gcgcttcctg ctcgacaagg ccgaggagga ccgcggcgcc
      781 cgctacctgc gcgacaccct gctgccgtcg ctggcctacg ccgcccgccg ggtgccggag
      841 atcgccgact ccctggtcga ggtcgaccac gccgtcgagt ggggcttcgg ctacgagctg
      901 ggcccgttcc gggcctggga cgccatcggc gtggccgacg gggtgggccg catggaggcc
      961 ctcgggatcg aggtcccggc ctgggtgacc gagatgctgg ccgccgggaa cgacgccttc
     1021 taccgcgacg gccaggtcta cagccccatc acccgcgcct acgagccggt gccggccgac
     1081 ccggaggtcc tggacctgga ggcgcgcaag gccggggggg ccgtggtggc ccagaacggc
     1141 tcggccagcc tggtcgacct gggcgacggg gtgctctgct tcgagctgca cgccccggcc
     1201 agcgccatcg acgccgccgt ggtcgagctc ggggcccggg ccctccagga gctggaggaa
     1261 ggcgactggg ccgggatggt gatcgccaac caggcccgca acttctgcgt cggggccaac
     1321 ctgggcgagg tcggcatggc cgcctaccag ggcctgtatg accaggtggg cgaggccgtc
     1381 aagggcctcc aggacgtgct catgggcttc cggcacgcgg cccggccggt ggtcgccgcc
     1441 ccccacggcc agacgctggg gggaggggcc gagatcgtcc tgcacgccga ccgggtggtg
     1501 gccgccctgg agacctacct cgggctggtc gaggtcgggg tcggcatcgt cccggccggg
     1561 ggcgggtgca aggagctgct gcgccgggtc gtcgccccgg ccgtccgggc cgccccggac
     1621 gcgcccgtgc tgccgttcgt gcaacgggtg ttcgagacca tcggccttgc caaggtcgcg
     1681 acctcggcgg tcgaggccca ggagctgggg ttcctggagg agcacgacgt gattgtgctc
     1741 aacgccgacc accagctggc ggcggccaag caggaggtcc tcgacctggc cgacgcctac
     1801 cgccccccgg cccgggaggc gtccatctac gcggccgggc tgcccgtcct ggccgccctg
     1861 gagctgggcg tgcagaccct ccagtgggcc ggccaggcca gcgagcacga cggcgtcgtc
     1921 gcccgccacc tggcccgggt cctgtgcggg ggcgagctgt cgctcggcca gtgggtgacc
     1981 gagcagcaca tcctcgacct ggagatggag gcgttcctgg ccctgctccg cgagcccaag
     2041 accatggagc ggatccaggg ctttctgatg acccgcaagg tcgtccgcaa ctgaggggat
     2101 agagcgatga gggaagctgt cattgtctcc ggggcccgga ccgcggtcgg ccgggcgccc
     2161 cgggggagcc tgcgggcgag ccggcccgac gacatggccg gggcggccat cgccgaggcc
     2221 ctgcgccggg ccgagggcct cgacccggcc gaggtcgagg acgtcctgct cggctgcgcc
     2281 atcaccgagg gcagccaggg gctgaacgtg gccaggatcg ccgcccagcg ggccggcctg
     2341 cccgagaccg tgcccgccca gacggtcaac cggttctgct cctcgggcct gcagacgatc
     2401 gccagcgcgg ccgagcggat catggccggg ttcgccacca ccatggtggc cggcgggacc
     2461 gagcacatgt cctcgacccc gacgcccacc ccgaccttcg cccccaaccc cgaggtcgtc
     2521 cgcggccgcc ccgagtacta catggccatg ggcctgaccg gcgagcgggt ggccgagcgc
     2581 tggggcgtca gccgcgagga ccaggacgcc ttcgccctcc gctcccacca gcgggcggcc
     2641 gaggcggtcg actcgggccg cttcgacccc gagatcgtcc ccctgcccgt ccgggtggac
     2701 accctggacg gcgacgggcg ggccgtcacc tccgagggca ccttcagccg cgacgagggg
     2761 ccgcgccgcg acacctcggc cgaggccctg gccaagctca agcccgtgtt caagcagggc
     2821 ggcaccgtca ccgccggcaa ctcctcccag cgcagcgacg gcgcggccgc ggtcgtggtc
     2881 atggagcggg aacgggccga gcgcctcggc cttgccccgc tgggccggtt cgtgggctac
     2941 gccgtcggcg gggtcgcccc cgaggtcatg ggcatcggcc ccaccgtggc catcccgaag
     3001 gtgctggagc tgaccggcct gcgcctggac gacatcgacc tgatcgagct caacgaggcg
     3061 ttcgcctccc agaccctggc cgtcatccgc gaggtcggcc tcgacctcga caaggtcaac
     3121 gtcaacgggg gcgccatcgc cctcggccac cccatgggcg ccaccggcac caagctcacc
     3181 gtccagctcc tggccgagct ggcccgccgc ggcggccgct acggcatggt caccatgtgc
     3241 gtcggcggcg gccagggcgc cgccgggatc ttcgagaacc tccagcgcta gccgccgggg
     3301 cggcctgtgg acagcgccgc gccctcattc cgctggcgag gtagcctccc ccaccggggg
     3361 acccgatgcc ggcgggtcgg gctgcaggcc gcccgggggt gcgggacgcc cggggcagct
     3421 cggcacgagc acgacccgtt cggcgtcgtg ccagccgagg ggcccgcagc ccagctccgg
     3481 aaccccgggg agcggtagag gcggaacccg ggctcctcca gctcgccggc cagctcctcg
     3541 gcgaagaacc cggcctcgac cgcggtctcg tggaagggcg cggcccccat ggccgcccgt
     3601 cgacgtcgac cagccgctcg ctgacggcgg ccgtcccggg ccgtccaggc ggtccaggcg
     3661 ggtcacgacg gcggccgggc gtcaccggcc ggggcggggg gacgggcgcc ggcgaactgg
     3721 cgggactggg cgaccaggcg gccggccgag tcc
//
LOCUS       DASSWR010000698         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_281018,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000698 DASSWR010000000
VERSION     DASSWR010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281018"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFO47_11720"
     CDS             complement(<1..239)
                     /locus_tag="VFO47_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HET8789403.1"
                     /translation="MTGPVASARAAFARQAWGDAHALLSAAPDLEPEDLERLAVAAYL
                     VGRDDESTVAWERAHGAWARLGETDRAARCAGWLGL"
     gene            complement(315..1268)
                     /locus_tag="VFO47_11725"
     CDS             complement(315..1268)
                     /locus_tag="VFO47_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8789404.1"
                     /translation="MTADQTVSEGAAAPRPPDAAEPATEPTGSAPPVATAASVGVRWW
                     REVLYVVLVYLAYSAVRNQFGSGGGSIDPDPAFHHGEAVIQLQRNIGLYFEHRLQRWY
                     LDLPANGLIRLWNVYYGVLHFVVTAFALVWMYRRAPARYRLWRNTLAITTLLALVGFA
                     SFSLMPPRLLDDPGIYGGCQVYADATPDELAARGVEAAGDPPCDEFGFVDTIAVYGGW
                     ASFGSDEMAAVSNQYAAMPSMHIGWSMWCSLVLVPMVWRRWLRGLAVAYPALTLFCIV
                     VTANHYWIDGLAGLACLGLGYLVARTATRWWESRRGVAAIA"
     gene            complement(1265..>2066)
                     /locus_tag="VFO47_11730"
     CDS             complement(1265..>2066)
                     /locus_tag="VFO47_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET8789405.1"
                     /translation="GIVLSALRWQKVLDALEIPTRLPRLIRHYLAGQFVSNVLPTTIG
                     GDVLRVSRLSRDTGESPGTFASVVLERLTGWLVLPVITIIGFAVNPGLRHLGTATRVA
                     LALAVGTLLLLVAVLVVAGSRRLGGRFAARPGWQRFVGAVHLGVDRLRRQPVAALNVL
                     VAGFAYQLALVLAAVAAAQALGLRPAGLTALLAFFPAVLIAQVLPISMSGLGVREGAF
                     VLFLGPLGVATDEAVALGLLLYLLNLAVSLLGAPAFAAGGRPRASVAA"
BASE COUNT          318 a          754 c          797 g          197 t
ORIGIN      
        1 aggcccagcc agcccgcgca gcgcgctgcc cggtcggtct cgccgaggcg ggcccaggcg
       61 ccgtgcgccc gctcccaggc cacggtgctc tcgtcgtcgc gcccgaccag gtaggcggcg
      121 acggcgaggc gctcgaggtc ctcgggctcg aggtccgggg cggcggacag cagcgcgtgg
      181 gcgtcacccc acgcctggcg agcgaacgcc gcccgggccg acgccacggg accggtcaca
      241 cggtccagtg tcgcccaccc ggacctggcc cgcaccgccg gcgggtgcgg gcctgcaccg
      301 tcccgagccg ccggtcaggc gatcgcggcg acgccgcgcc gggactccca ccagcgcgtg
      361 gccgtgcggg cgaccaggta gccgaggccc aggcaggcga ggccggccag cccgtcgatc
      421 cagtagtggt tggccgtgac gacgatgcag aagagggtca gtgccgggta ggcgacggcg
      481 agccccctca gccaccgccg ccagaccatc ggcaccagga cgagcgagca ccacatcgac
      541 cagccgatgt gcatgctcgg catggccgcg tactggttgg acaccgccgc catctcgtcg
      601 ctgccgaacg acgcccagcc gccgtagacg gcgatggtgt cgacgaaccc gaactcgtcg
      661 cagggcgggt cgccggccgc ctcgacgccc cgggcggcga gctcgtcagg ggtggcgtcc
      721 gcgtagacct ggcagccgcc gtagatgccg gggtcgtcca gcagccgcgg cggcatgagc
      781 gagaagctcg cgaacccgac gagcgccagc agggtggtga tggccagcgt gttgcgccac
      841 agccggtagc gggccggcgc ccgccggtac atccagacga gcgcgaacgc ggtcacgacg
      901 aagtgcagca ccccgtagta gacgttccag agccggatca gcccgttcgc cggcaggtcg
      961 aggtaccacc gctgcagccg gtgctcgaag tagagcccga tgttgcgctg cagctggatg
     1021 acggcctcgc cgtggtggaa cgccgggtcc gggtcgatgc tgccgccgcc cgagccgaac
     1081 tggttccgca ccgccgagta ggccaggtag acgaggacga cgtagaggac ctcgcgccac
     1141 caacgcacgc cgacggacgc cgcggtggcg accggcggcg cggacccggt cggctcggtc
     1201 gccggctcgg cggcatccgg cggccggggc gctgccgcac cctccgagac ggtctggtcg
     1261 gccgtcatgc cgccacgctc gcacggggcc gcccaccggc ggcgaaggcc ggcgccccga
     1321 gcaggctgac ggccaggttc agcaggtaga gcagcagccc gagggcgacg gcctcgtcgg
     1381 tggccacgcc cagcggcccg aggaacagca cgaaggcgcc ctcgcgcacc ccgaggcccg
     1441 acatcgagat gggcagcacc tgggcgatga gcacggccgg gaagaacgcc agcagggcgg
     1501 tgaggccggc cgggcgcagg ccgagcgcct gggccgccgc cacggcggcg agcacgagcg
     1561 ccagctggta ggcgaacccg gcgacgagca cgttgagggc cgcgaccggc tggcggcgca
     1621 gccggtcgac gccgaggtgg acggccccga cgaaccgctg ccagccgggc cgggcggcga
     1681 agcggccgcc gagccggcgg ctgccggcca cgaccagcac ggccaccagc agcagcagcg
     1741 tgccgacggc cagcgccagc gccacccggg tggcggtgcc gaggtgccgg agccccgggt
     1801 tgacggcgaa gccgatgatc gtgatgaccg gcaggaccag ccagccggtc agccgctcga
     1861 gcacgaccga cgcgaacgtg cccggcgact cgccggtgtc gcgcgacagg cggctgaccc
     1921 gcagcacgtc gccgccgatg gtggtgggga gcacgttgga gacgaactgc ccggccaggt
     1981 agtggcggat gagccggggg agccgggtgg ggatctccag ggcgtcgagc accttctgcc
     2041 agcgcagggc cgacagcacg atgccg
//
LOCUS       DASSWR010000699         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1382395, whole genome shotgun sequence.
ACCESSION   DASSWR010000699 DASSWR010000000
VERSION     DASSWR010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382395"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFO47_11735"
     CDS             <1..825
                     /locus_tag="VFO47_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789406.1"
                     /translation="RGHLAEGRRWLAEALAMPSGQAATAARAKALTGAGGIAWWSDQA
                     AAHSFYDEALAIERELGDPARLAEALYNEAFAVAAEHDQDAAARLFDESLTLFRQVGD
                     EHGVARVLVMRVVPDAMAGAWDRVIARIEEVTVIWRRQGERLNLAFGLIWLAFAHGRA
                     GRRDDARATALEALELFREADNATGVALAFLDLAFLLTWEGRHADAIRMAAVAEHHRD
                     LAGGGPTPGFGSMLEGDPAADARAHLTDEAAQRAWQEGLTMTVPEAIRVVRDDPGA"
     gene            complement(927..1859)
                     /locus_tag="VFO47_11740"
     CDS             complement(927..1859)
                     /locus_tag="VFO47_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET8789407.1"
                     /translation="MSGPVGTADAPPAESRASSVAPACVEVTPSRMADVGGMPVRRAL
                     PRRERRTVGAWCFVDHMGPAPVTETAGLDIGPHPHTGLQTVTWLLSGEVLHRDSLGTE
                     QLIRAGQLNLMTAGHGVAHSEEATGYYRGELHGVQLWVAQPERTRHGPAAFEHHPELP
                     RAEFGHAVATVLVGSFAGVTSPARRDSALVGVDLALRPGTGALALEPSFEHALVVRDG
                     RVLVHDQPVRPGRLAYLGRGRDQLELSADDPARVLLLGGEPFAEPILMWWNFVARTRE
                     EITTAARQWRAADPRFGRVASPLARLRLPPLPWR"
BASE COUNT          247 a          737 c          814 g          234 t
ORIGIN      
        1 cgcggccacc tggccgaggg ccgccgctgg ctcgcggagg ccctggccat gccctccggg
       61 caggccgcca ccgccgccag ggccaaggcc ctgaccgggg cgggcgggat cgcctggtgg
      121 agcgaccagg cggcggcgca cagcttctac gacgaggccc tggccatcga gcgggagctc
      181 ggcgacccgg cccgcctcgc cgaggctctc tacaacgagg ccttcgccgt ggccgccgag
      241 cacgaccagg acgccgcggc ccgcctgttc gacgagagcc tgacgctgtt ccgccaggtc
      301 ggggacgagc acggggtggc cagggtcctg gtgatgcgcg tcgtcccgga cgcgatggcc
      361 ggcgcctggg accgcgtgat cgcccgcatc gaggaggtca cggtgatctg gcgccggcag
      421 ggcgagcgcc tcaacctcgc cttcggcctg atctggctgg cgttcgccca cggccgcgcc
      481 ggccgccgcg acgacgcccg cgccaccgcc ctcgaggccc tggagctgtt ccgcgaggcc
      541 gacaacgcca ccggggtcgc cctcgccttc ctcgacctgg cgttcctgct gacctgggag
      601 ggccgccacg ccgacgccat ccggatggcc gccgtggccg agcaccaccg cgacctggcc
      661 ggcggcggcc ccacccccgg cttcggcagc atgctcgagg gcgacccggc ggccgacgcc
      721 cgcgcccacc tgaccgacga agcggcccag cgggcctggc aggaggggct cacgatgacc
      781 gttcccgagg cgatcagggt ggtccgggac gacccggggg cctgaggcgg cgctgctggc
      841 gatcggcgga ggcgacgtta tggtccccgc gggtatcgcg gtcacagggg acctgcagcc
      901 ggtggacccg atgggtggcg gctcccctag cgccagggca gcgggggaag gcggagtcgg
      961 gccagggggg aggcgacccg gccgaagcgg gggtctgcgg cgcgccactg gcgggcggcg
     1021 gtggtgatct cctcgcgggt tcgggcgacg aagttccacc acatcaggat gggctcggcg
     1081 aacggctcgc cgcccaggag caggaccctg gcggggtcgt cggcggacag ctcgagctgg
     1141 tcgcggccgc ggcccaggta ggccaggcgg cctgggcgga ccggctggtc gtggaccagg
     1201 acgcggccgt cgcgcacgac cagggcgtgc tcgaacgacg gctccagggc cagggcgccc
     1261 gttcccgggc gcagggccag gtcgaccccg accagggcgc tgtcgcgccg ggccggggag
     1321 gtgacgccgg cgaagctgcc gaccaggacg gtggcgacgg cgtggccgaa ctcggcccgg
     1381 ggcagctcgg ggtggtgctc gaaggcggcg gggccgtggc gggtccgctc gggctgggcg
     1441 acccacagct gcacgccgtg gagttcgccg cggtagtagc cggtggcctc ctcggagtgg
     1501 gcgacgccgt gcccggccgt catgaggttc agctggccgg cccggatgag ctgttcggtg
     1561 ccgaggctgt cgcggtgcag cacctcgccc gacagcagcc aggtgacggt ctgcaggccg
     1621 gtgtgcgggt gggggccgat gtccaggccg gccgtctcgg tcaccggcgc cgggcccatg
     1681 tggtccacga agcaccaggc gccgacggtg cgccgctccc gtcgcggcag ggcccggcgc
     1741 accggcatcc cgccgacgtc ggccatgcgg ctgggggtga cctcgacgca ggccggcgcg
     1801 accgagctcg ccctcgactc ggcggggggt gcgtcggcgg tgccgaccgg accgctcatg
     1861 tccctcactg tggccgcggg accccgccgg cgcaaccgca ggcggtaggg tgggcagcca
     1921 actggcgacg aaccgggaga gacaacctat ggacgtgttc cggcgccccc tcggcgcggc
     1981 cggccgggtc gccctggcgt ccatgttcat caccggcggc gccgacgcga tg
//
LOCUS       DASSWR010000700         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_224113,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000700 DASSWR010000000
VERSION     DASSWR010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224113"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..954
                     /locus_tag="VFO47_11745"
     CDS             22..954
                     /locus_tag="VFO47_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8789408.1"
                     /translation="MSDLPTVPVASKAPTRRRGRRVQATGTDRIFLALMVIIPTALVV
                     GLVWLPAVATVVLSFASWTGVGSLDTIKWIGTDNYVNVFTIYPPFKPALQHNLIWLVF
                     LFVIPTVLGIFLAVLLDRELRGTRFYQSALFLPVVLSLALVGFIWQLIYSRDQGLLNA
                     VLHTEIDWYGDSRWNLWAVLVAAGWKHTGYIMLLYLAGLKGVDPSLREAAAVDGASPA
                     RTFFSVVFPVMRPINIIIIVVTVIEALRAFDIVWVINKGTNGLELISTLVTANVVGEA
                     SRIGFGSALATIMLVISLVFITIFLSTVMRGERR"
     gene            951..1868
                     /locus_tag="VFO47_11750"
     CDS             951..1868
                     /locus_tag="VFO47_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8789409.1"
                     /translation="MTSTTVTRGATSEATAAGTRRRRLSPARVLLHVFLVATALAWLF
                     PVVWAVFNSFRDYDYTSEHGYVSLGGFTFQNYLNAWEQGEFTRHFLNSLYITVPAVLL
                     TLFLASCVAFVVARFSFRFNLILLGLFTAANLLPQQALLIPLFRLYREVPLPLWMSDS
                     GQLLDSYWGLILVNTAFQTGFCTFVLSNYMKALPQEMFEAARVDGASVPTQFFRITLP
                     LCRPALAALATLQVTWIYNEFYWATVLMQSGDKFPITSSLTNLRGQFFTDYNLLSAGS
                     VVIAIPVLVVFFLLQKQFVAGLTLGSTKG"
     gene            1865..>2046
                     /locus_tag="VFO47_11755"
     CDS             1865..>2046
                     /locus_tag="VFO47_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789410.1"
                     /translation="MSPPGVLQLRAGGVSLVLDAPAGTGSPLPVVLHWGPDLGENLDG
                     DALAGALVPAAAHSAI"
BASE COUNT          269 a          737 c          655 g          385 t
ORIGIN      
        1 tcctgacacc ggggaggcgc gatgagcgac ctgccgaccg tccccgtcgc ctccaaggcg
       61 ccgacacgcc ggcgcggccg ccgtgtccag gcgaccggca ccgaccggat cttcctggcg
      121 ctcatggtga tcatccccac cgcgctggtg gtcgggctgg tgtggctgcc cgcggtcgcc
      181 accgtggtgc tgtcgttcgc cagctggacc ggcgtcggca gcctggacac catcaagtgg
      241 atcgggaccg acaactacgt caacgtgttc accatctacc cgccgttcaa gccggccctc
      301 cagcacaacc tcatctggct ggtgttcctg ttcgtcatcc ccaccgtgct cgggatcttc
      361 ctggccgtgc tgctggaccg cgagctgcgc gggacccgct tctaccagag cgccctgttc
      421 ctgccggtgg tcctttcgct ggccctggtc gggttcatct ggcagctcat ctactcgcgc
      481 gaccaggggc tcctcaacgc cgtgctccac accgagatcg actggtacgg ggactcccgc
      541 tggaacctgt gggcggtgct ggtcgccgcc ggctggaagc acaccggcta catcatgctg
      601 ctctacctgg ccgggctgaa gggcgtcgac cccagcctgc gggaggcggc cgccgttgac
      661 ggggccagtc cggcccggac cttcttctcg gtcgtgttcc cggtgatgcg tcccatcaac
      721 atcatcatca tcgtggtcac ggtgatcgag gccctgcggg ccttcgatat cgtctgggtg
      781 atcaacaagg gcaccaacgg cctggagctg atctcgaccc tggtcacggc caacgtcgtc
      841 ggggaggcga gccggatcgg cttcggctcg gccctggcca ccatcatgct ggtcatctcg
      901 ctggtgttca tcaccatctt cctgtccacc gtcatgcggg gggagcggcg atgaccagca
      961 ccaccgtcac ccgcggggcc accagcgaag cgactgcggc cgggaccagg cggcggcgcc
     1021 tgtcgccggc gcgggtcctg ctgcacgtgt tcctggtcgc gaccgccctg gcctggctgt
     1081 tcccggtggt gtgggcggtg ttcaactcct tccgcgacta cgactacacc tcggagcacg
     1141 gctatgtctc cctgggcggg ttcacgttcc agaactacct gaacgcctgg gagcagggcg
     1201 agttcacccg ccacttcctc aactcgctgt acatcaccgt gccggcggtg ctactgacgc
     1261 tgttcctcgc ctcctgcgtg gcctttgtcg tcgcccgctt cagcttccgg ttcaacctga
     1321 tcctgctggg gttgttcacc gcggccaacc tgctgccgca gcaggcgctg ctgatcccgc
     1381 tgttccggct ctaccgcgag gtcccgctgc cgctgtggat gagcgactcg gggcagctgc
     1441 tggacagcta ctgggggctg atcctggtca acaccgcctt ccagaccggc ttctgcacct
     1501 tcgtgctcag caactacatg aaggccctgc cgcaggagat gttcgaggcg gcccgggtcg
     1561 acggggccag cgtccccacc cagttcttcc gcatcaccct gccgctgtgc cggccggccc
     1621 tggccgcgct ggccaccctc caggtcacct ggatctacaa cgagttctac tgggccacgg
     1681 tgctgatgca gtcgggcgac aagttcccca tcaccagctc cctcacgaac ctgcggggcc
     1741 agttcttcac cgactacaac ctgctctcgg ccgggtcggt ggtcatcgcc atccccgtcc
     1801 tggtggtgtt cttcctgctc cagaagcagt tcgtggccgg cctgaccctg gggtcgacca
     1861 aggggtgagc ccaccgggag tgctccagct ccgcgccggc ggggtctcgc tggtgctgga
     1921 cgcgccggcc gggacggggt cgcccctgcc ggtggtgctg cactggggcc cggacctggg
     1981 cgagaacctc gacggcgacg ccctggccgg ggcgttggtg ccggcggcgg cgcactcggc
     2041 catcga
//
LOCUS       DASSWR010000701         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_592398,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000701 DASSWR010000000
VERSION     DASSWR010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592398"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFO47_11760"
     CDS             <1..442
                     /locus_tag="VFO47_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789411.1"
                     /translation="FAGQSIPLRHHQEHGGSFETVQRIASVAGGRQGVFLWEQSSSLY
                     DVAYLFGGPVWLQQGQISALLPRRPDPAYVQSFVRGFPGQPVFLVTKGHGRPQAYPGL
                     SLRAVDQITYVMPVWRETYTTRPSDAVGVPLRFTIWQVGSHTAA"
     gene            complement(482..1114)
                     /gene="lexA"
                     /locus_tag="VFO47_11765"
     CDS             complement(482..1114)
                     /gene="lexA"
                     /locus_tag="VFO47_11765"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET8789412.1"
                     /translation="MAEQLTTRQRRILEVIRDAVADRGYPPSIREIGEAVGLTSTSSV
                     HSQLEALQRKGFIRRDPTKPRAIEVHLEEPVAAMPRPLPTYVPLMSAPVAAGTGTLPE
                     DVVEELLPLPRELVGQGQLMMLQVRGESMVGAGVLDRDYVVVRRQDTADNGDMVAALL
                     PNDESEATVKHFSRRGGHIQLLPDNPAFDPIDGDQAQILGKVVAVLRRLR"
     gene            1252..1611
                     /locus_tag="VFO47_11770"
     CDS             1252..1611
                     /locus_tag="VFO47_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET8789413.1"
                     /translation="MIEQAFAQTFAKVGEAGRGEVSRMRLTRRGRMLVRVLAVVLVVA
                     GFLLVAPGLARGDGPDRPAPRVTYVVESGDTLWSIARRVAPGQDPRPVVDGLIEANHL
                     RGGLQAGQELSIPVPER"
     gene            complement(1729..2226)
                     /gene="nrdR"
                     /locus_tag="VFO47_11775"
     CDS             complement(1729..2226)
                     /gene="nrdR"
                     /locus_tag="VFO47_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020609027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET8789414.1"
                     /translation="MRCPFCGSDEDRVVDSRPSEDGGTIRRRRACAVCGRRFTTFERV
                     EEAPLLVLKRDGSREQFELGKLVSGLEKACKNRPIPREEILRIASDIEEAVRARGQRE
                     VESQEVGIELLNALRELDQVAYMRFASVYKDFQDPADFERELEDLGSLRKTTPPKPRP
                     SARVT"
     gene            2503..>3372
                     /locus_tag="VFO47_11780"
     CDS             2503..>3372
                     /locus_tag="VFO47_11780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572347.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET8789415.1"
                     /translation="MAKTRDALSVEHGPARRGLQVRRLFTTKGTHPYDELTWERRDAV
                     ITNWRDGTTAFEQRDLEFPAGWSQNATNIVAQKYFRGPLGTPQREHSVRQMVDRVAGT
                     ITRWGAKDGYFATDGDAEAFQAELTHLLVNQKAAFNSPVWFNVGVEPEPQCSACQPYD
                     ALVSTPTGMVPIGELVERQAIGTVVYDAHGETRVRAVKANGDKRVFRVLLRNGSFVET
                     TGDHLVYAVPERRSAGSWLRVDQLRPGMRLHLYPQRGSADDLIGPLATAERQAEVAGG
                     TATLVRTDPMTSEA"
BASE COUNT          474 a         1202 c         1174 g          522 t
ORIGIN      
        1 cttcgctggc cagtcgattc cgctccgcca ccaccaggag catggcggct cgttcgagac
       61 cgtccagcgg atcgcttcgg tggccggggg ccggcaaggg gtcttcctct gggagcagtc
      121 ctcaagcctc tacgacgtcg cctacctgtt cggcgggccg gtctggctgc agcagggcca
      181 gatcagtgcg ttgctccctc ggcggccaga tcccgcctat gtccagtcct tcgtccgcgg
      241 cttccccggc cagcccgtct tcctcgtcac caagggccac ggtcgccccc aggcctaccc
      301 tggcctgagc ctccgggcgg tcgaccagat cacctacgtc atgccggtgt ggcgggagac
      361 atacacgacc cgcccttctg atgcggtcgg cgtccctcta cggttcacca tctggcaggt
      421 cgggtcgcac accgccgcat agcccgcatc tcccgatgcc gggctcgtct caggtgcaca
      481 cctagcggag gcggcggagg acggcgacca ccttgccgag gatctgggcc tggtcgccgt
      541 cgatggggtc gaaggcgggg ttgtcgggga ggagctggat gtggccgccc cggcgggaga
      601 agtgcttgac cgtggcctcg gactcgtcgt tggggagcag ggcggcgacc atgtcgccgt
      661 tgtcggccgt gtcctggcgg cggacgacca cgtagtcgcg gtccaggacc ccggcgccga
      721 ccatcgattc cccccggacc tgcagcatca tgagctggcc ctgcccgacc agctcgcgcg
      781 gcaggggcag cagctcctcg acgacgtcct cgggcagggt gccggtaccg gcggcgaccg
      841 gggcgctcat caggggcacg taggtgggca ggggccgcgg catggccgcc accggctcct
      901 ccaggtggac ctcgatggcc cgtggcttgg tcgggtcgcg gcggatgaag cccttgcgct
      961 ggagcgcctc gagctgggag tggacgctgg aggtggaggt caggccgacc gcctcgccga
     1021 tctcgcggat ggacggcggg tagccccggt cggcgaccgc gtcgcggatc acctccagga
     1081 tgcgtcgttg cctggtggtg agctgctccg ccatcgctgt accccctcgc ccgaaggacc
     1141 cgttgccgcc tgatgaccgg actctagcag cggcgttggc tcgaagcaaa caccagttcg
     1201 atgtggtggg cgaggttgac agcgaacagg tgttcgggca ggatggaggg catgatcgaa
     1261 caggctttcg cccagacgtt cgccaaggtc ggcgaggcag gacgagggga ggtcagccgg
     1321 atgcgtctca cgaggcgcgg ccggatgctc gtccgggtgc tcgccgtcgt gctggtcgtc
     1381 gccgggttcc tcctggtcgc tcccggcctg gccaggggcg acgggcccga ccggccggcg
     1441 ccgagggtca cctacgtggt cgagtccggc gacaccctct ggtcgatcgc gcggcgggtc
     1501 gccccggggc aggacccgcg gccggtggtg gacggcctga tcgaggccaa ccacctccgc
     1561 ggcgggctcc aggccggcca ggagctgtcg atccccgttc cggagcgcta ggtccgggcg
     1621 gcgagctcca cgaccgtctt ctcccggtca tgggtcccgc cccccggccc gctcccatca
     1681 cgcccacgca ttctcggggc cgcgacagcc cgggccggcc ccgacccgct acgtcaccct
     1741 ggcgctggga cggggcttcg ggggggtggt cttgcggagg gaaccgaggt cctccagctc
     1801 gcgctcgaag tcggccgggt cctggaagtc cttgtagacg ctggcaaagc gcatgtaggc
     1861 gacctggtcc agctcgcgca gggcgttgag gagctcgatc ccgacctcct ggctctcgac
     1921 ctcgcgctgg cccctggccc ggaccgcctc ctcgatgtca ctggcgatgc gtaggatctc
     1981 ctcgcgaggg atcggccggt tcttgcaggc cttctccagg cccgacacga gcttgccgag
     2041 ctcgaactgc tccctggagc cgtcacgctt gaggaccagc agtggcgcct cctcgacccg
     2101 ctcgaaggtg gtgaagcgcc gaccgcagac cgcgcaggca cgccggcgac ggatcgtccc
     2161 gccgtcctcg gacggccggg agtcgaccac gcggtcctcg tcggacccgc agaaggggca
     2221 acgcacggct gatcctccct gggtcggccg gtgccgacca ccacaacata tagtggtata
     2281 atcggtctca ggaggcaagt tttccacaat atcttgtgtc gagggtgctt gacccgggcc
     2341 ccggatcggt gcttaatcac atcgctgtag ttcgtggatc tgtccgttcg tcgtcccgct
     2401 cggctcagct gacccggtcc gcagcaccgt tcagcgccgg cttctccagg gacggaggtg
     2461 cgcggccccg gccagcacgg gggtcgcagg aggtcaatca tcgtggccaa gacccgggat
     2521 gccctgtccg tcgagcacgg tcccgcccgc cggggccttc aggtgcgacg cctgttcacg
     2581 accaagggaa cccaccccta tgacgagctg acctgggagc gcagggacgc cgtcatcacc
     2641 aactggcgcg atggcaccac cgccttcgag cagcgcgacc tcgagttccc cgccggctgg
     2701 tcgcagaacg ccaccaacat cgtcgcccag aagtacttcc gcggccccct cggcaccccc
     2761 cagcgcgagc actccgtccg ccagatggtg gacagggtcg ccggcaccat cacccgctgg
     2821 ggcgccaagg acggctactt cgccaccgac ggcgacgccg aggccttcca ggccgagctg
     2881 acccacctgc tggtcaacca gaaggccgcc ttcaactccc cggtctggtt caacgtcggg
     2941 gtcgagcccg agccccagtg ctcggcctgc cagccctacg acgcgctggt cagcaccccg
     3001 accggcatgg tgccgatcgg tgagctggtc gagcgccagg cgatcgggac cgtcgtctac
     3061 gacgcccacg gcgagacccg ggtgcgggcg gtcaaggcca acggcgacaa gcgcgtgttc
     3121 cgggtcctcc tccgcaacgg ctcgttcgtc gagacgaccg gcgaccacct ggtgtacgcc
     3181 gtgcccgagc ggcggagcgc cggttcgtgg ctgcgggtcg accagctccg gccggggatg
     3241 cgcctgcacc tgtacccgca gcgtgggtcc gccgacgatc tgatcggccc gctcgcgacc
     3301 gccgagcggc aggccgaggt cgcaggcggg acggcgacct tggtccgcac cgatccgatg
     3361 acgagcgagg cg
//
LOCUS       DASSWR010000702         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_24691,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000702 DASSWR010000000
VERSION     DASSWR010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24691"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VFO47_11785"
     CDS             complement(<1..168)
                     /locus_tag="VFO47_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET8789416.1"
                     /translation="MAGSRRARAARRRKRRMDRVDHDLSDAQWTALKAAWGGCAYCGA
                     SNTPLQRDCVQA"
     gene            306..575
                     /locus_tag="VFO47_11790"
     CDS             306..575
                     /locus_tag="VFO47_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET8789417.1"
                     /translation="MDIIGVIIAGIIIGLLGKFFAPGDRDNIPLWLTIVCGVGGVLIG
                     YYLAAALGVEATRGIDWIRWIISIIVAAVLVMIAASVTGRRGRTI"
     gene            847..1125
                     /locus_tag="VFO47_11795"
     CDS             847..1125
                     /locus_tag="VFO47_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789418.1"
                     /translation="MPASHDNLLTPTEHEAAQLLVTLAWRLDGGQLVSTDQMLARLCS
                     IPTNDVAMASIVLLREVAHSVAPALQRPPIEILDHFRVWLAGRAGASG"
     gene            complement(1155..2012)
                     /locus_tag="VFO47_11800"
     CDS             complement(1155..2012)
                     /locus_tag="VFO47_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789419.1"
                     /translation="MGVIVLCIVMVAAGLLAIARWGAMAVQPPPTPAGYEPGPDGRPP
                     VGLVVRRYLWYLTLAVSAGAAAGLLAAGAGGRLVMRLLAVTAGPGAQGRITEADQVVG
                     RISVDGTLGFIIFTGLLFGAASGAAYLVLRRWLPAGRAGGAVFGALLLVLAGVRLEPL
                     RPGNPDFDLVGPGWVSVAAFTALVLFHGMLVAALAGRLSRSIPLLAPTAPAIAAHLPL
                     LLLALGSVALVAAMVGAVAVLASQIPAVVAAWRDQRALTAGRVVLGLVALVALPSFAS
                     AVVDILGRP"
     gene            complement(2003..>2451)
                     /locus_tag="VFO47_11805"
     CDS             complement(2003..>2451)
                     /locus_tag="VFO47_11805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET8789420.1"
                     /translation="ADLREPDALMGHPEVRELIDFNQPIALLLMAILHFVPDDEDPYG
                     IVARFRDALPAGSYLAISHGTRDIPVRPDMSPEEMTEMGTKVERLYQLTTASLVTRTH
                     AEVERFFDGLDLLDPGVVEIQRWRPDGRRPMLPGGFYGGVGRKPWG"
BASE COUNT          388 a          898 c          867 g          298 t
ORIGIN      
        1 cgcctggacg cagtctcgct gcagcggggt gttggaggca ccgcagtagg cgcacccacc
       61 ccacgccgcc ttgagcgcgg tccactgcgc atcgctgagg tcgtggtcga cacgatccat
      121 ccggcgcttg cgccggcgag cggctctcgc cctacgactc ccggccacca ccgtcacccc
      181 agtgcaggca cccagcctcc ggctcctcat cgccgcgctg gaacgagatg gccagggtcc
      241 gcgacctcag ttgtaggatg atctccatgc tggattgagc cctcggctcg gcgaagggag
      301 tcgctgtgga catcatcggt gtgatcatcg caggcatcat catcggcctg ctgggcaagt
      361 tcttcgcgcc aggcgaccgc gacaacattc cgctctggct caccattgtc tgcggggtcg
      421 gcggcgtgct catcggctac tacctcgccg cagcgctcgg tgtcgaggcg acgcgcggca
      481 tcgactggat ccggtggatc atcagcatca tcgtcgcggc cgtcctcgtc atgatcgcgg
      541 cctcggtgac gggaaggcgc ggcaggacga tctaaggagt cctgggaccc gaggaccagg
      601 tcctcggcaa cgcacctgcc gttggccagg gacagcccgt ccatacaggg ggctgtccct
      661 ggcccagacc ccgccagggc tcccccggac aaggcgaccg cccaggcgag gctcgcttgt
      721 cggggcagcc ggccgggctg cagcagcaag ccccggtgca gaacccgata aggaggaggt
      781 cgccgaccgt ggcatagcat gcgcggatcc ggccggacag cggctccagg ccaggaggga
      841 caaggcatgc ccgcgtcgca tgacaacctc ctcaccccaa ccgagcacga ggcagcccag
      901 ctactcgtca ccctcgcctg gcggctggac ggcggacagc tcgtcagcac cgaccagatg
      961 ctggccaggc tctgcagcat cccgaccaac gacgtcgcca tggcgagcat cgtgctgctc
     1021 cgggaagtgg cgcacagcgt cgcacccgcg ctccagcggc cgccgatcga gatcctcgac
     1081 cacttccgtg tgtggctcgc cggccgagcc ggggcgtcgg gctaacgcgg ctcgatccgt
     1141 ccctgcccat caccctacgg ccggcccagg atgtcgacga cggccgaggc gaagctggga
     1201 agggccacca gggcgaccag accaaggacc acccgtccgg ccgtcagcgc gcgctggtcg
     1261 cgccaggcag cgaccacggc cgggatctga ctggccagca cggccacggc gccgaccatg
     1321 gccgccacca gggcgaccga gcccagcgcg agcagcagca gcggcaggtg ggcggcgatg
     1381 gcgggagcgg tgggggcgag cagcgggatc gaccggctga gccggccggc cagggcggcg
     1441 accagcatgc cgtggaagag caccagcgcg gtgaaggcgg ccaccgacac ccatcccggc
     1501 cccaccaggt cgaagtcggg gttgcccggg cgcaggggct ccagccggac gccggccagg
     1561 accaggagca gggcgccgaa gacggcgcca ccggcccgcc cggcgggcag ccagcgccgc
     1621 agcaccaggt aggcggctcc cgaggccgcc ccgaagagca ggccggtgaa gatgatgaac
     1681 ccgagcgtgc cgtcgacgct gatgcgcccc acgacctggt cggcctcggt gatccgtccc
     1741 tgcgcgccgg gcccggccgt caccgccagc agccgcatca ccagccgccc gccggctccg
     1801 gcggccagca ggccggcggc ggcgcccgcg ctgacggcca gggtcaggta ccagaggtag
     1861 cggcgcacga cgaggccgac gggcgggcgg ccgtccgggc cgggctcgta ccctgccggc
     1921 gtgggcggcg gctggaccgc catggcgccc cagcgggcga tcgcgagcag tccggcggcc
     1981 accatcacga tgcagaggac gatcaccccc atggcttgcg gccgaccccg ccgtagaacc
     2041 cgcccggcag catcgggcgc cggccgtcgg gcctccaccg ctggatctcg accacgcccg
     2101 ggtcgagcag gtcgaggccg tcgaagaacc gctcgacctc cgcgtgcgtg cgggtcacca
     2161 gcgacgcggt ggtcagctgg tacagccgct cgaccttcgt ccccatctcg gtcatctcct
     2221 ccggagacat gtccggccgc accgggatgt ccctggtgcc gtgggagatg gccaggtagc
     2281 tgccggccgg cagggcgtcc cggaaccggg ccacgatccc gtaggggtcc tcgtcgtcgg
     2341 ggacgaagtg cagaatcgcc atgagcagca gcgcgatcgg ctggttgaag tcgatgagct
     2401 cccgaacctc gggatgcccc atgagggcgt cgggctcccg gaggtcggcc t
//
LOCUS       DASSWR010000703         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_140410,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000703 DASSWR010000000
VERSION     DASSWR010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140410"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFO47_11810"
     CDS             complement(<1..631)
                     /locus_tag="VFO47_11810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HET8789421.1"
                     /translation="MEARADGEAPDRNLALELVRVTEAAAMAAARKMGFGDKEAVDQA
                     AVDAMRHVLGSVSMTGVVVIGEGEKDNAPMLYNGEEIGDGTPPEVDIAVDPVDGTTLT
                     AKSMPNAISVVALSDRGTMFDPGPCVYMEKLVVGPEAADAVDFEAPLALNLRLVAKAK
                     GGHVADLTVCILDRPRHEEELVGRVRATGARIKFLLDGDVAGAIMAAREE"
     gene            854..1849
                     /locus_tag="VFO47_11815"
     CDS             854..1849
                     /locus_tag="VFO47_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET8789422.1"
                     /translation="MEGTVGGSQGHGHGHSHAPVPLAGSAAGEDEGLRAVKLSAAGLG
                     LTAAVQFAFVAASGSVALLADGLHNLGDVFTTVTLWIAFRVGRRQPDRGYTFGYARAE
                     DVAGVLVVLAIAASAVVAAAESLAKLAGDVPPLRNPGWALAAAFAGVAGNEAVAQYKL
                     RVGRRINSVPLEADGQHSRVDGLASLAAAAGIAGAWAGWPAADPLAGLLLSAVIGWVL
                     VGTTRDVLARLLDRVDPEVIDEVERAAWSVAGVEAVHAARARWVGRSLHVLVHVEVDP
                     DLPLRAAHDLGEQVRHAIFHALPGVAAVDLHLDPAGVDEHESHGHTLHHRDQGSR"
     gene            complement(1881..>2068)
                     /locus_tag="VFO47_11820"
     CDS             complement(1881..>2068)
                     /locus_tag="VFO47_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789423.1"
                     /translation="GSCPVRVAGSGFARPCDGQRWDAEGGPASGLRDGEEAPPPLRRV
                     AAAFAGGDLYVNPAASP"
BASE COUNT          220 a          786 c          786 g          276 t
ORIGIN      
        1 tctcctcgcg ggcggccatg atcgccccgg cgacgtcacc gtcgagcagg aacttgatcc
       61 gcgcgccggt ggcccggacc cggccgacca gctcctcctc gtggcggggg cggtcgagga
      121 tgcagacggt caggtcggcg acgtgcccac ccttggcctt ggcgaccagg cggaggttga
      181 gggccagcgg ggcctcgaag tcgaccgcgt cggccgcctc cggcccgacc accagcttct
      241 ccatgtagac gcacggtccc gggtcgaaca tggtgccgcg gtccgagagg gccaccaccg
      301 agatggcgtt gggcatgctc ttggcggtca gggtggtgcc gtcgaccggg tcgacggcga
      361 tgtcgacctc gggcggggtg ccgtcgccga tctcctcgcc gttgtagagc atgggggcgt
      421 tgtccttctc gccctcgccg atcaccacca ccccggtcat cgacaccgag ccgagcacgt
      481 ggcgcatggc gtcgacggcc gcctggtcga cggcctcctt gtcgccgaag cccatcttgc
      541 gggccgcggc catggcggcc gcctcggtga cccggacgag ctcgagggcc aggttgcggt
      601 cgggggcctc gccgtcggct cgagcttcca tcgcacacct ccgcgtcggt tccgggccgg
      661 cgttggcccc gggggaccat tttccccacc ggagggactg gcatccaggg ccgctccggc
      721 ttaccctgac aacggtggcc ccgcccgctc gggtgtgcgt ggcctccggc agtcgggccc
      781 tggtgccgcc gatcccaccc ggttggcgcc atgggcccgc tgcgtgtccg gcgcccgata
      841 gggtgttgcg gccatggagg gaacggtggg cggcagccag gggcacgggc atgggcacag
      901 tcacgccccg gtgccgctgg ccggctcggc ggccggcgag gacgaggggc tgcgggcggt
      961 caagctgtcg gccgccggcc tcgggctgac cgccgccgtc cagttcgcct tcgtggccgc
     1021 cagcggctcg gtcgccctgc tcgccgacgg cctccacaac ctgggcgacg tgttcaccac
     1081 cgtcaccctc tggatcgcct tccgggtcgg gcggcgccag ccggaccgcg gctacacctt
     1141 cgggtacgcc agggccgagg acgtcgccgg ggtgctggtc gtcctggcca tcgccgcctc
     1201 ggcggtggtc gccgccgccg agtcgctggc caagctggcc ggggacgtgc cgccgctgcg
     1261 caaccccggc tgggcgctgg ccgcggcgtt cgccggggtg gccggcaacg aggccgtggc
     1321 ccagtacaag ctccgggtcg gccggcggat caactcggtc ccgctggagg ccgacggcca
     1381 gcactcgcgg gtcgacgggc tggccagcct ggccgccgcg gccgggatcg ccggggcctg
     1441 ggccggctgg ccggccgccg acccgctggc cggcctgctg ctgtcggccg tgatcggctg
     1501 ggtgctggtc gggaccaccc gcgacgtgct cgcccggctg ctcgaccggg tcgacccgga
     1561 ggtcatcgac gaggtcgagc gggcggcctg gtcggtcgcc ggggtcgagg ccgtgcacgc
     1621 ggcccgggcc cgctgggtgg gccggtccct gcacgtcctg gtccacgtcg aggtcgaccc
     1681 cgacctgccc ctgcgggccg cccacgacct cggcgagcag gtccgccacg ccatcttcca
     1741 cgccctcccc ggcgtggccg cggtcgacct gcacctggac ccggccgggg tcgacgagca
     1801 cgagagccac ggccacaccc tccaccaccg cgatcagggc agccggtagc gtctccggaa
     1861 ggcggccacg ccgagcgggc tcacgggctc gctgccgggt tgacgtacag gtcgccgccc
     1921 gcgaacgcgg ccgcgacccg gcgcagcggg ggcggggcct cctcgccgtc gcgcaggccg
     1981 ctggccgggc cgccctcggc gtcccagcgc tggccgtcgc aggggcgggc gaacccgctg
     2041 ccggcgaccc gcaccgggca ggagccgg
//
LOCUS       DASSWR010000704         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1382623, whole genome shotgun sequence.
ACCESSION   DASSWR010000704 DASSWR010000000
VERSION     DASSWR010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382623"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFO47_11825"
     CDS             complement(<1..342)
                     /locus_tag="VFO47_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET8789424.1"
                     /translation="MSLLAIAWTIVAFLVGALVVVLCVVMANLFRVLTSTKDLIDGVT
                     SQTVPLLGEVNTTVAMVNQELTRVDGILATAEGVTASVGGMVNTVSATISSPLVKLSA
                     FAYGLRKAAGAA"
     gene            complement(480..1886)
                     /locus_tag="VFO47_11830"
     CDS             complement(480..1886)
                     /locus_tag="VFO47_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8789425.1"
                     /translation="MRERRRWTLVVVCLATAVLLLNVAAPNVALPDIGADLHAELSVL
                     QWVISGYSLALAATLLTAGTLADLVGRRRMFLGGLAGFAAASMLCALAPSALVLIAGR
                     AAQGLAGAVLLSSSLALLAQDFTGVARAGAIGVWGATVAAAFAIGPLEGGLLTEAFGW
                     RAIFVLDVAIALACLPVAVRRLRESRDPDAGQVDWWGTVTLCLGLFLGVFALTRGDVL
                     GWGSGVILASAAAAAALLAAFVVVERREPEPMLDLGLFATPTFTGASVVVLLLAASTF
                     GPFLYLTLFLLDVGGASPTAVGLQLMPLSAAAFVVSSLGGRYARVLPVRAALPAGQLL
                     SAAGLLAMRGVEASSPWTFLLPGLLLMGVGIGLSNPAVTYAALGVVPATRSGMASGTN
                     NTFRQVGLAIGIAVLGTLLPAQAEADPAAFAAALDRILLVSAAVAAAGAVLAVTLIRQ
                     RDFVVDPVPAGSQSDRAA"
     gene            complement(1883..2176)
                     /locus_tag="VFO47_11835"
     CDS             complement(1883..2176)
                     /locus_tag="VFO47_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET8789426.1"
                     /translation="MADECTHLDQIRISSVNQPGCQDCLATGGTWVELRLCVGCGRVG
                     CCDNSPNRHATKHFQSTGHPIIRSYEPDERWWWCYVDELAFELEGVEPARASG"
     gene            complement(2252..>2784)
                     /locus_tag="VFO47_11840"
     CDS             complement(2252..>2784)
                     /locus_tag="VFO47_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789427.1"
                     /translation="LAAASLARAAMEGQDPSGRPLFAAHAALPWPEPPHLALWHAATL
                     YREFRGDAHVACLTAEGIDGLEAHVLAAGAGQLPGAVLREYRGWSDEEWAAAVERLGA
                     RGWVDPGGSLTKAGRAARDARERRTDDLAMPPWRRLGPGGCARLLDLLGPPVRLVVDG
                     GGIPFPNPVGLPAPMA"
BASE COUNT          418 a         1011 c         1081 g          274 t
ORIGIN      
        1 ggcggcgccg gccgccttgc ggagcccgta ggcgaacgcg ctcagcttga ccagagggct
       61 ggagatggtg gccgagacgg tgttgaccat gccgcccacc gaggcggtga cgccctcggc
      121 ggtggccagg atgccgtcga cccgggtgag ctcctggttg accatggcca cggtggtgtt
      181 gacctcgccc agcagcggga ccgtctggga ggtgacgccg tcgatcaggt ccttggtcga
      241 ggtcagcacc cggaacaggt tggccatcac gacacacagc acgacgacca gggcgccgac
      301 caggaaggcg acgatcgtcc aggcgatggc cagcaggctc acgggttctg ctcctcgttg
      361 tccgtcgggt ccggaacgct ccagcttagc cggtccaacc cggtccggcg cgccaggttc
      421 gggggagcgg caagctggag ccgccgggag tctaccgcga ccgtggaggg agcaggacct
      481 cacgccgccc ggtcggactg ggagccggcc gggaccgggt cgacgacgaa gtcccgctgg
      541 cggatcaggg tcacggccag gaccgccccg gcggcggcga cggcggcgct gaccagcagg
      601 atccggtcga gcgcggccgc gaaggcggcc gggtcggcct cggcctgggc cgggagcagc
      661 gtccccagga cggcgatgcc gatggccagg ccgacctggc ggaaggtgtt gttggtgccc
      721 gacgccatgc cgctgcgggt cgccgggacc accccgaggg cggcgtaggt cacggccggg
      781 ttggacaggc cgatcccgac ccccatgagc agcaggccgg gcagcagaaa ggtccagggc
      841 gaggacgcct ccaccccccg catggccagc aggccggccg cggacaggag ctggccggcg
      901 ggcagggccg cccgcaccgg caggaccctg gcgtagcgcc cgccgaggga ggagaccacg
      961 aaggcggccg ccgacagggg catgagctgg agcccgaccg cggtcgggga ggccccgccc
     1021 acatccagca ggaacagcgt caggtacagg aacggtccga aggtggaggc ggccaggagc
     1081 agcaccacca ccgaggcccc ggtgaaggtc ggggtggcga acaggccgag gtcgagcatg
     1141 ggctcgggct cgcgccgctc caccaccacg aacgcggcca gcagggccgc cgccgcggcc
     1201 gcgctggcca ggatgacccc gctcccccag ccgagcacgt cgccacgggt gagggcgaac
     1261 accccgagga acagccccag gcagagcgtc accgtgcccc accagtcgac ctggccggcg
     1321 tcggggtcgc gggactcgcg caggcgccgc acggccaccg gcaggcaggc cagggcgatg
     1381 gcgacgtcga gcacgaagat ggcccgccag ccgaacgcct cggtgagcag cccgccctcc
     1441 agcggcccga tggcgaaggc ggccgccacc gtcgcccccc acaccccgat ggccccggcc
     1501 cgcgccaccc cggtgaagtc ctgggccagc agggccagcg acgaggacag cagcaccgcc
     1561 ccggccagcc cctgggcggc ccgcccggcg atcaggacca gcgccgaggg cgccagggcg
     1621 cacagcatcg aggcggccgc gaacccggcc agcccgccga ggaacatccg ccgccgcccc
     1681 accaggtcgg ccagggtccc ggcggtgagc agggtcgcgg ccagggcgag cgagtagccg
     1741 ctgatgaccc actggaggac gctcagctcg gcgtgcaggt ccgctccgat gtcgggcagg
     1801 gcgacgttgg gggcggccac gttcagcagc agcacggcgg tggccaggca caccaccacc
     1861 agggtccagc ggcggcgctc cctcacccgc tggctcgggc cggctcgacg ccctcgagct
     1921 cgaaggccag ctcgtccacg tagcaccacc accagcgctc gtccggctcg tagctccgga
     1981 tgatcgggtg cccggtcgac tggaagtgct tggtggcgtg ccggttgggc gagttgtcgc
     2041 agcaccccac ccgcccgcag cccacgcaca accgcagctc cacccaggtc cccccggtgg
     2101 ccaggcagtc ctggcacccg ggctggttca cgctcgagat gcggatctgg tcgaggtggg
     2161 tgcactcgtc ggccatcacg gcgctccaag gatgtgggga ggactctcaa cggggccaac
     2221 agcgcctcgg ggcgccgcat tccggttccc gtcaggccat gggggcggga aggcccaccg
     2281 ggttggggaa cgggatgccg ccgccgtcga cgaccaggcg gaccggaggg ccgaggaggt
     2341 cgagcaggcg ggcgcagccg ccggggccga ggcggcgcca ggggggcatg gccaggtcgt
     2401 cggtgcgccg ttcgcgggcg tcgcgggcgg cccggccggc cttggtcagc gatccgccgg
     2461 ggtcgaccca gccgcgggcg ccgagccgct ccacggcggc ggcccactcc tcgtcggacc
     2521 agccgcggta ctcgcgcagg accgccccgg gaagctggcc ggcgccggcg gccaggacgt
     2581 gggcctcgag gccgtcgatc ccctcggccg tcaggcaggc cacgtgggcg tcgccgcgga
     2641 actcgcggta cagggtggcg gcgtgccaca gggcgaggtg gggcggctcc ggccagggca
     2701 gggcggcgtg ggcggcgaac agcgggcggc ccgacgggtc ctggccctcc atggccgccc
     2761 gggccagcga cgcggccgca agag
//
LOCUS       DASSWR010000705         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_564721,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000705 DASSWR010000000
VERSION     DASSWR010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564721"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFO47_11845"
     CDS             <1..826
                     /locus_tag="VFO47_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8789428.1"
                     /translation="QDARAAPAERVHASGRLADALVPLGLLQARRGDPEAAATLQEAT
                     EAAFATGELQWTGQVAAARAEDAWLHGDDERVAEEAGRVHEAAVRAGHPWFAGELAFW
                     LWLAGTPRPVTGTVAEPYRLLLAGDWRAAAGAWRELGCSYHRALALACGDRDEAPLEA
                     LALLDGLGARQTAQRLRRQLRRRGDLHVPRGPNRATAANPAGLTGRQVEVLGLLAEGL
                     TDAEIATRLSLSAKTVGHHVSALLAKLGVGSRRQAAAAARRLGLVPARDGDPGGQS"
     gene            975..1781
                     /locus_tag="VFO47_11850"
     CDS             975..1781
                     /locus_tag="VFO47_11850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8789429.1"
                     /translation="MDIATIKQKQQAAWASGDYAAVGTRLLLTAELLCEAVDLRAGEQ
                     VLDVACGHGNAALAAARRFCQVTGVDYVPALLERARQRAAAEGLEVSFQEGDAEDLPF
                     GDASFDVVLSTCGAMFAPDQERTAAELLRVCRPGGRIGMVNWVPDGYIGELFRTIGRY
                     LPPPPGLRPPVEWGSQQRLRELFGPEATISAPRRTFRWRWPSPEHQAEFFATFYGPTN
                     RVVAAIGDDRAADLKADMAKVAAGFNLSDDDTLVLRQEYLEVVVHKPATD"
     gene            1792..2802
                     /locus_tag="VFO47_11855"
     CDS             1792..2802
                     /locus_tag="VFO47_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET8789430.1"
                     /translation="MPASTVKVAAVQAAPVFLDLEASLVKALALIEQAADGGARLLVF
                     PEAFLPAWPAWVDEVLPGEDEAWHLRLLKQSVVVPGPVTERLGAAARDAGVHLVRGVD
                     EREEHGGTVYNTVLYFDDGGRLLGKHRKLVPTHAERLVWGMGDGSDLAVHQTAVGRVG
                     GLICWENYMPLARFALYAQGVDIWVAPTLATHEPWVATMRHIAREAACHVIGVAPAAR
                     FSEVPESVPDRERLWRGVEPRGDWMLDGYSVIVDPAGTVLAGPLVREEGILAADLDLD
                     AARARRRLFDPVGHYNRPDVFRLVVDDRPNPTWWACPPRPGPVHPGPDRLTGPAARPW
                     PP"
BASE COUNT          352 a         1126 c         1054 g          351 t
ORIGIN      
        1 gcaggacgcc cgggcggcgc cggccgagcg ggtgcacgcc agcggccggc tggcggacgc
       61 gctggtgcca ctgggcctgc tgcaggcacg ccggggcgac cccgaggccg cggccaccct
      121 gcaggaggcc accgaggccg ccttcgccac cggcgagctg cagtggaccg ggcaggtggc
      181 cgccgcccgg gccgaggacg cctggctgca cggcgacgac gagcgggtcg ccgaggaggc
      241 cggccgcgtg cacgaggcgg ccgtccgggc cgggcacccg tggttcgcgg gggagctggc
      301 cttctggttg tggctggcgg gcacgccgcg gccggtgacg ggaacggtgg ccgagccgta
      361 ccggctgctg ctggccggcg actggcgcgc cgccgccggc gcctggcggg agctcggctg
      421 ctcctaccac cgggccctgg ccctggcctg cggcgaccgg gacgaggcgc cgctggaggc
      481 gctggcgctg ctggacggcc tcggcgcccg ccagacggcc cagcggctcc ggcgccagct
      541 ccggcggcgc ggcgacctgc acgtcccccg cggcccgaac cgggccacgg ccgccaaccc
      601 ggccggcctc accggccgcc aggtcgaggt gctcggcctg ctggccgagg gcctgaccga
      661 cgccgagatc gccacccggc tgtcgctgtc ggccaagacg gtcggccacc acgtctcggc
      721 gctgctggcc aagctcggcg tcgggtcacg ccgccaggcc gccgcggccg cccgccggct
      781 cggcctcgtc ccggccagag atggggaccc gggcggccag agttagggaa ccgatcccga
      841 tcccccgccc ccggtggcgt cctagcgttc cctggtcaca cgtcggctca cacaccgctg
      901 ccggatgctg cgaccccagg gcggccgcca gccacctcga cccagctcac cgccccgacc
      961 atgggaggta cggcatggac atcgccacca tcaagcagaa gcagcaggcc gcctgggcct
     1021 ccggcgacta cgcggccgtc gggacccggc tgctgctcac cgccgagctg ctgtgcgaag
     1081 ccgtcgacct ccgcgccggc gaacaggtcc tggacgtggc ctgcggccac ggcaacgccg
     1141 ccctggccgc cgcccgccgc ttctgccagg tcaccggcgt cgactacgtc cccgcgctgc
     1201 tggaacgagc ccgccagcgc gccgccgccg aagggctgga ggtcagcttc caagaaggcg
     1261 acgccgagga cctgcccttc ggtgacgcct ccttcgacgt cgtcctgtcg acctgcgggg
     1321 ccatgttcgc ccccgaccag gaacgcaccg cggccgagct gctgcgggtc tgccgccccg
     1381 gcggccgcat cggcatggtc aactgggtcc ccgacggcta catcggcgag ctgttccgga
     1441 ccatcggccg ctacctgccc ccaccgccgg ggctgcggcc cccggtggaa tggggcagcc
     1501 agcagcggct gcgcgagctg ttcggccccg aggccaccat ctcggccccc cgccggacct
     1561 tccgctggcg ctggccttcg cccgaacacc aggccgagtt cttcgcaacc ttctacggcc
     1621 ccaccaaccg ggtcgtggcc gccatcggcg acgaccgcgc cgccgacctc aaggccgaca
     1681 tggccaaggt ggccgccggc ttcaacctct ccgacgacga caccctggtc ctgcgccagg
     1741 agtacctgga ggtcgtcgtc cacaagcccg cgacggacta ggggaacgct catgccggcg
     1801 tcgacggtga aggtggccgc ggtccaggcc gccccggtgt tcctggacct ggaggccagc
     1861 ctggtcaagg cgctggccct gatcgagcag gcggccgacg ggggggcccg cctgctcgtc
     1921 ttccccgagg cgttcctgcc cgcctggccg gcctgggtcg acgaggtcct gcccggcgag
     1981 gacgaggcct ggcacctgcg gctgctaaag cagtcggtgg tggtgcccgg cccggtgacc
     2041 gagcggctgg gcgcggccgc ccgcgacgcc ggcgtccacc tggtcagggg ggtggacgag
     2101 cgcgaggagc acggcggcac cgtctacaac acggtcctgt acttcgacga cggcggccgg
     2161 ctgctgggca agcaccgcaa gctggtcccg acccacgccg agcggctggt ctggggcatg
     2221 ggggacggct ccgacctggc tgtccaccag accgcggtgg gccgggtggg cgggctgatc
     2281 tgctgggaga actacatgcc cctggcccgc ttcgccctct acgcccaggg ggtcgacatc
     2341 tgggtcgcgc ccaccctggc cacccacgag ccctgggtgg ccaccatgcg ccacatcgcc
     2401 cgcgaggccg cctgccacgt catcggggtc gccccggcgg cccggttctc cgaggtgccc
     2461 gagagcgtgc ccgaccggga gcggctgtgg cgaggggtgg agccgcgcgg cgactggatg
     2521 ctggacgggt actcggtgat cgtcgacccc gccgggacgg tgctcgccgg accgctggtc
     2581 cgcgaggagg gcatcctcgc cgccgacctc gacctggacg ccgcccgggc ccgccgccgc
     2641 ctgttcgacc cggtgggcca ctacaaccgc cccgacgtgt tccgcctggt cgtcgacgac
     2701 cgcccaaacc ccacctggtg ggcctgtcca cccaggccgg gcccggtgca ccctggccct
     2761 gaccgcctga ctggacctgc tgctaggccc tggccgccgt gaccagccgt tcgacgtccc
     2821 ggaggtcgcg caggtaggtc aggccgggca cggcgggtgc ctccgcgttg cggtcgagga
     2881 cga
//
LOCUS       DASSWR010000706         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_760902,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000706 DASSWR010000000
VERSION     DASSWR010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760902"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFO47_11860"
     CDS             complement(<1..383)
                     /locus_tag="VFO47_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8789431.1"
                     /translation="MRVGMVLVVVAAVVAATVGLLWVFQRRLIYLPSGSVPPAASVLS
                     GAEDVSFETADGLRLGGWFVPGAGGRGPAVRVCNGNGGNRAMRAALAAALARMGLAVL
                     LFDYRGYGGNPGSPTEEGLAADARAA"
     gene            451..1287
                     /locus_tag="VFO47_11865"
     CDS             451..1287
                     /locus_tag="VFO47_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8789432.1"
                     /translation="MEVGVGLPTTTPGADGRTVLEWARRAEAGPFASLGVLDRMVYRS
                     VEPFAALAAAAAVTTRVRLVTMVVIGPLRNTVLLAKQAASLDLLSAGRLTLGLAIGAR
                     ADDYQALGVDQAGRGRRLGEQLAELRSIWEKGEVGPEPVQPGGPPLLAGGLSGEAFAR
                     MARGADGYVHGGGPPRAFAGAAARAWAAWRDLERPGRPQLWGQGYFALGEPDAGEAYL
                     RDYYAFTGPFAAKIAAGNLTSGRAVKDFVRGYEDAGCDHLVLLPTVSDPAQLDRLADV
                     LG"
     gene            1300..>2951
                     /locus_tag="VFO47_11870"
     CDS             1300..>2951
                     /locus_tag="VFO47_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8789433.1"
                     /translation="MRIGVLGGGPAGLYFALLAKRADPAHEITVVERNAPDATFGWGV
                     VFSEETLGALRDADYPSYVEITDTFARWSAIDVVYGGRTVRSGGHVFSAIARRRLLEI
                     LQRRCREVGVELVFQREVPDLEGFAGRDLVVAADGANSTARRLLEPHLRPTQDVHRSR
                     FIWFGTDLPLDAFTFIFRTTDHGMFQVHAYPFDADTSTFIVECTEGTWRAAGLDQAGE
                     SESIAFCQELFAPELGGHKLLSNRSLWISFVTVRCRSWHHGNVVLLGDAAHTAHFTIG
                     SGTKLAMEDAVALGQAVARHPADLEAAFTDYELERQPVVERFQEAARDSATWFENVRR
                     YDGFDPVQFACNLLTRSGRIGHLELTRRDPAFAAAVDRRFAGRDGLLLAPPPLFAPLE
                     QRAVRLANRVVLAPVGEDDADDGRLPAEAVARLEMAAGAGAGAVVTELVAVAPDGRVT
                     PGTPGLWADDQAGRWADAARAVEGQGSALVLRHGHAGRRGATRPRRAGVDRPLPAGGW
                     PLLAASAVPYTPASQVPAAMTPADMGRVVGEFAAAAGRAAAAG"
BASE COUNT          341 a         1181 c         1077 g          352 t
ORIGIN      
        1 gccgcccggg cgtcggcggc cagcccctcc tcggtcgggc tgcccgggtt cccgccgtac
       61 ccgcggtagt cgaacagcag caccgccagc cccatccggg ccagcgcggc cgccagcgcg
      121 gcccgcatgg cccggttccc cccgttgccg ttgcacaccc gcaccgccgg cccccgccct
      181 ccagcgccgg ggacgaacca gccgccgagg cggaggccgt cggcggtctc gaagctgacg
      241 tcctcggcgc cggagaggac cgaggcggcc gggggcaccg accccgacgg gaggtagatc
      301 aggcggcgct ggaacaccca gagcagcccc accgtggccg ccacgaccgc ggccacgacc
      361 accaggacca tcccgacgcg catccctgag ccgtaccatg atcgtcgctc gggcgcacca
      421 cttctgaacc agcggcgggg aggagcgagc atggaggtcg gggtcgggct gcccacgacc
      481 acgccgggcg ccgacgggcg caccgtcctg gagtgggcca ggcgggccga ggcggggccg
      541 ttcgccagcc tgggcgtgct cgaccggatg gtgtaccgca gcgtcgagcc gttcgcggcc
      601 ctggccgcgg cggccgccgt cacgacccgg gtccggctgg tcaccatggt ggtgatcggg
      661 ccgctgcgca acaccgtgct cctggccaag caggccgcct ccctcgacct gctgtcggcc
      721 ggccggctca ccctcgggct ggccatcggg gcccgggccg acgactacca ggcgctcggc
      781 gtcgaccagg ccggccgcgg ccggcgcctg ggcgagcagc tggccgagct gcgctccatc
      841 tgggagaagg gcgaggtcgg tcccgagccg gtccagccgg gcggcccgcc gctgctggcc
      901 ggcgggctgt caggggaggc gttcgcccgc atggcccgcg gcgccgacgg ctacgtccac
      961 ggcggcggcc cgccccgggc cttcgccggc gccgccgcca gggcgtgggc ggcctggcgg
     1021 gacctggagc ggccgggccg cccccagctc tggggccagg gctacttcgc cctcggcgag
     1081 cccgacgccg gcgaggccta cctgcgcgac tactacgcct tcaccggccc gttcgcggcc
     1141 aagatcgccg ccggcaacct caccagcggc cgggccgtca aggacttcgt ccgcggctac
     1201 gaggacgccg gctgcgacca cctggtcctg ctgcccaccg tctccgaccc ggcccagctc
     1261 gaccgcctgg ccgacgtgct cggctgagag gaggccggca tgcgcatcgg ggtcctgggc
     1321 ggcggcccgg ccgggctgta cttcgccctg ctggccaagc gggccgaccc ggcccatgag
     1381 atcaccgtgg tcgagcgcaa cgcccccgac gctaccttcg ggtggggggt ggtgttctcg
     1441 gaggagaccc ttggggcgct gcgcgacgcc gactacccga gctacgtgga gatcaccgac
     1501 accttcgccc gctggagcgc catcgacgtg gtctacggcg gccgcaccgt ccgctccggc
     1561 gggcacgtgt tctcggccat cgcccgcagg cggctgctgg agatcctgca gcggcgctgc
     1621 cgcgaggtcg gggtcgagct ggtcttccag cgggaggtcc ccgacctgga ggggttcgcc
     1681 ggccgcgacc tggtggtcgc cgccgacggg gccaactcca ccgcccggcg gctgctggag
     1741 ccgcacctgc gcccgaccca ggacgtgcac cggtcgagat tcatctggtt cggcaccgac
     1801 ctgccccttg acgccttcac cttcatcttc cgcacgaccg accacggcat gttccaggtc
     1861 cacgcctacc ccttcgacgc cgacaccagc accttcatcg tcgagtgcac cgagggcacc
     1921 tggcgggcgg ccgggctcga ccaggccggc gagtccgaga gcatcgcgtt ctgccaggag
     1981 ctgttcgccc ccgagctggg cgggcacaag ctgctctcca accggtcgct gtggatcagc
     2041 ttcgtcaccg tccgctgccg gagctggcac cacggcaacg tcgtcctgct cggcgacgcc
     2101 gcccacaccg cccacttcac catcgggtcg ggcaccaagc tggccatgga ggacgcggtc
     2161 gccctcggcc aggccgtggc gcgccacccg gccgacctgg aggccgcctt caccgactac
     2221 gagctggagc gccagccggt ggtcgagcgc ttccaggagg cggcccgcga cagcgccacc
     2281 tggttcgaga acgtgcgccg ctacgacggc ttcgacccgg tccagttcgc ctgcaacctg
     2341 ctgacccgct cgggccgcat cggccacctg gagctgaccc ggcgcgaccc cgccttcgcg
     2401 gccgccgtgg accggcgctt cgccggccgg gacggcctcc tgctggcccc gccgccgctg
     2461 ttcgcgcccc tggagcagcg ggcggtgcgc ctggccaacc gggtcgtcct ggcccccgtc
     2521 ggcgaggacg acgccgacga cggccgcctg cccgccgagg ccgtcgcgcg cctcgagatg
     2581 gcggccgggg ccggggccgg ggcggtggtg accgagctgg tcgcggtcgc ccccgatggc
     2641 cgcgtcaccc ccggcacccc gggcctgtgg gccgacgacc aggccggtcg gtgggccgac
     2701 gcggcccggg cggtcgaggg acagggctcg gccctggtcc ttcgccacgg ccacgccggg
     2761 cgccgcggcg ccacccggcc ccgccgggcc ggggtcgacc ggccgctgcc ggccgggggc
     2821 tggccgctgc tggccgcctc ggccgtcccc tacaccccgg ccagccaggt cccggcggcg
     2881 atgacgccgg ccgacatggg gcgggtcgtc ggcgagttcg ccgccgccgc cggccgggcc
     2941 gccgccgccg g
//
LOCUS       DASSWR010000707         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_224338,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000707 DASSWR010000000
VERSION     DASSWR010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224338"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFO47_11875"
     CDS             <1..182
                     /locus_tag="VFO47_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789434.1"
                     /translation="FTVKELPQIKGIYAFAVAVRDPATNVTYDARRFSEAFLAVGDTG
                     TGVLEVVWTTEQVER"
     gene            199..1554
                     /locus_tag="VFO47_11880"
     CDS             199..1554
                     /locus_tag="VFO47_11880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8789435.1"
                     /translation="MAEPLVTVVVLNWNGAHLLPDCLRGLAAQDLPEGQARTWVVDNA
                     STDGSLELLADEFPWVRAIANGSNDGFAGGNNVALREVSTPFVALVNNDARPEPDWLR
                     RLLEPFSEPGAERLGAVSGKIVFLPRFLAVELATPGFLPGTLDTRELGVRVYRVEVDG
                     ADVTERVLWDRAAYGPEGEGPGRFRWTRPSGLLLIPVDRPEGDGPLRLRFRVAAETTK
                     AVELTWPGGSGAGKAEPEPVELEVEVPAGVPLVDVLNNAGSMVFRDGYGADRAYQELD
                     RGQYQRPEEVFAFCGGSVCFRTEALRQAGVFDDDFFLYYEDTDLSWRLRSLGWRIRYQ
                     PAAVARHIHSASSVEWSPLFVFHTDRNRLLMLTKNARAGLAAREVLRYPLTTASLALR
                     EVARARHTRRRPPVRPTLLRLRVLGSYARLLPAMLARRRRIAARAAVDRRRLERWLVL
                     R"
     gene            1551..2795
                     /locus_tag="VFO47_11885"
     CDS             1551..2795
                     /locus_tag="VFO47_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET8789436.1"
                     /translation="MRVGLDATPLLGPRTGVGRYVAGLAGALATLAGPEPEELALVPF
                     SWRGTRDLPAVASQGTGDVPGVARDGPRVRYGRRRAPARLLQAAWARLPWPPVEWLSG
                     PVEVFHGTNFVAPPTRRAATVVTVHDLTYLRFPEMVTDASARYRDLVPRALRRGAVVC
                     TPTAAVATEVAAEYGLPPDRLVVTPLGVDPSWRQATPPDPSWLATHGLPGRYLLFVGN
                     REPRKNLTGLLTAYRQLLSTRVTSPPRVGGVGTGPTAGEGAGTTTAPAAGGVPPLVLV
                     GPPGWGETLDTAGLPAGAVHTPGYLPELDLVRVVAGAAALVVPSWYEGFGLPALEALA
                     CGVPVVASDLPAHREVLGDQAELIPPGDPAALAAALARVLDDPGGEPVRAARRARAAG
                     FTWENCAQATLSAYRLALESRR"
     gene            2792..3982
                     /locus_tag="VFO47_11890"
     CDS             2792..3982
                     /locus_tag="VFO47_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET8789437.1"
                     /translation="MTATPPGGEPAAAPGERGPGLGGPRVGVVAEQLLRPVPGGVGRY
                     VRALATHLPVEAAVDRGAVEFLVARHPPDRLAAVGLPAGTWQLAWPGRLVTRTWVTLR
                     RPALPGGLLDQLDLVHATSAAVPPTGSRPLVATVHDLAFRHFPDAYPASGRRYHERSA
                     RIVADEAARVLVPSEATARDLAELYGVDRGRVTITPLGVEVPPEPDRAGAERLLRDLG
                     VRGPFLLAVGTLEPRKNLPRLLDAFGEVADELPGHWLVVVGPVGWGPRLRPTWDSVRV
                     KLAGPVGDALLHAIYQAADGLAYPSLYEGFGLPVLEAMANGTPVLTSDRSSLPEVAGD
                     AALLVDPLDRSAIAAGLVRLAGDEDLRRRLVEAGRRRAAGFTWRATAAATWATYREVA
                     ERRP"
BASE COUNT          404 a         1607 c         1476 g          515 t
ORIGIN      
        1 gcttcaccgt caaggagctg ccgcagatca agggcatcta cgccttcgcg gtggccgtcc
       61 gtgaccctgc caccaacgtg acctacgatg cccgtcggtt cagcgaggcc ttcctggccg
      121 tcggcgacac cggcaccggc gtgctcgagg tcgtctggac caccgagcag gtcgagcgtt
      181 aggagaagcc cgttggccgt ggccgagccg ctggtgaccg tggtggtgct gaactggaac
      241 ggcgcccacc tgctccccga ctgcctgcgg gggctggccg cccaggacct gcccgagggg
      301 caggcccgga cctgggtggt cgacaacgcc tccaccgacg ggtcgctgga gctgctcgcc
      361 gacgagttcc cctgggtgcg ggccatcgcc aacggcagca acgacgggtt cgccgggggc
      421 aacaacgtcg ccctgcgcga ggtctcgacc ccgttcgtgg ccctggtcaa caacgacgcc
      481 cggcccgagc cggactggct gcggcggctg ctggagccgt tctccgagcc gggggcggag
      541 cggctcgggg cggtctcggg caagatcgtg ttcctgcccc ggttcctggc cgtcgagctg
      601 gccacccccg ggttcctgcc cggcaccctc gacaccaggg agctgggggt gcgggtgtac
      661 cgggtcgagg tcgacggcgc cgacgtcacc gagcgggtgc tgtgggaccg ggccgcctac
      721 gggccggagg gggaggggcc ggggcgcttc cgctggaccc gcccgtcggg gctgctgctc
      781 atccccgtcg accgcccgga aggtgacggg ccgctgcggc tgcggttccg ggtcgcggcc
      841 gagacgacca aggcggtgga gctgacctgg cccggtgggt cgggggccgg caaggccgag
      901 ccggagccgg tcgagctgga ggtcgaggtt ccggccgggg tgccgctggt cgacgtgctc
      961 aacaacgccg gcagcatggt gttccgcgac ggctacgggg ccgaccgggc ctaccaggag
     1021 ctggaccggg gccagtacca gcggcccgag gaggtgttcg ccttctgcgg cggctcggtc
     1081 tgcttccgca ccgaggcgct gcgccaggcc ggggtgttcg acgacgactt cttcctctac
     1141 tacgaggaca ccgacctgtc gtggcggctg cggtcgctcg gctggcgcat ccgctaccag
     1201 ccggcggcgg tggcccgcca catccactcg gcctcctcgg tcgagtggtc gccgctgttc
     1261 gtgttccaca ccgaccgcaa ccggctgctc atgctgacca agaacgcccg ggccgggctg
     1321 gccgcccgcg aggtcctgcg ctatccgctg accaccgcct ccctggccct gcgggaggtc
     1381 gccagggccc gccacacccg ccgccgccct ccggtccgcc cgaccctgct gcgcctgcgg
     1441 gtcctcgggt cgtatgcgcg cctgctcccg gccatgctgg cccgccgccg ccgcatcgcc
     1501 gcccgggccg cggtcgaccg ccgacgcctg gagcgctggc tggtgctgcg gtgagggtcg
     1561 gcctcgacgc gaccccgctg ctcgggccgc ggacgggcgt cggccgctac gtggccggac
     1621 tggccggggc gctggccacc ctggccgggc cggagccgga ggagctggcc ctggtcccgt
     1681 tctcctggcg gggcacccgc gacctgccgg ccgtggcctc gcagggcacc ggcgacgtgc
     1741 ctggtgtggc ccgggacggc ccgcgcgtcc ggtacgggcg ccggcgggcc ccggcccgcc
     1801 tgctccaggc cgcctgggcc cgcctgccct ggccgcccgt cgagtggctc tcgggcccgg
     1861 tcgaggtgtt ccacggcacc aacttcgtcg ccccgccgac ccggcgggcc gccaccgtcg
     1921 tcaccgtcca cgacctcacg tatctacgct tccccgagat ggtgaccgac gcctcggccc
     1981 gctaccgcga cctcgtcccc cgcgccctcc gccggggcgc ggtcgtctgc acccccaccg
     2041 ccgccgtcgc caccgaggtc gccgccgagt acggcctccc ccccgaccgc ctggtcgtca
     2101 cccccctcgg cgtcgacccc tcctggcgcc aggccacccc gcccgacccg tcctggctcg
     2161 ccacccacgg cctccccggc cgctacctcc tgttcgtcgg caaccgcgaa ccccgcaaga
     2221 acctcacggg cctgctcacc gcctaccgtc aactcctctc gacccgggtg acctcccctc
     2281 cccgggtggg aggggttgga accggcccga cggcaggtga gggggccggg acgaccacgg
     2341 ccccagcggc gggcggcgtg cctcccctcg tcctggtcgg cccccccggc tggggcgaga
     2401 ccctggacac cgcgggactg ccggcggggg cggtgcacac accgggctat ctcccggagc
     2461 tcgacctcgt ccgcgtcgtc gccggggccg ccgcgctggt cgtcccctcc tggtacgagg
     2521 gcttcggcct ccccgccctg gaggcgctgg cctgcggtgt cccggtggtc gcctccgacc
     2581 tccccgccca ccgcgaggtc ctgggcgacc aggccgagct catccctccc ggcgaccccg
     2641 ccgccctggc cgccgccctg gcccgggtcc tggacgaccc cggcggcgag cccgtccggg
     2701 cggcccggcg cgccagggcg gccggcttca cctgggagaa ctgcgcccag gcgaccctgt
     2761 ccgcctaccg cctggccctg gagtcccgcc ggtgaccgcg accccgcccg gtggggagcc
     2821 ggcggccgcg ccgggggagc gggggccggg cctgggcggt ccccgggtcg gggtggtggc
     2881 cgagcagctg ctgcgacccg tccccggcgg ggtcgggcgc tacgtgcggg cgctggccac
     2941 ccacctgccg gtggaggcgg ccgtcgaccg gggcgcggtc gagttcctgg tcgcccgcca
     3001 cccgcccgac cggctggccg cggtcggcct cccggcgggg acctggcagc tggcctggcc
     3061 gggccggctg gtcacccgga cctgggtgac cctgcgccgc ccggccctgc ccggcgggct
     3121 gctcgaccag ctcgacctgg tccacgcgac cagcgccgcc gtgcccccga cggggagcag
     3181 gccgctggtc gcgaccgtcc acgacctggc cttccggcac ttccccgacg cctacccggc
     3241 gtccgggcgc cgctaccacg agcggtcggc ccggatcgtg gccgacgagg cggcccgggt
     3301 gctggtcccg tcggaggcga ccgcccgcga cctggccgag ctgtacggcg tcgaccgggg
     3361 ccgggtcacc atcaccccgc tcggggtcga ggtcccgccc gagcccgacc gggccggggc
     3421 cgagcggctc ctgcgcgacc tgggcgtccg cgggccgttc ctgctggccg tgggcaccct
     3481 ggagccgcgc aagaacctgc cccggctgct ggacgccttc ggcgaggtgg ccgacgagct
     3541 gcccgggcac tggctggtgg tggtgggccc ggtcgggtgg gggccgcggc tgcgcccgac
     3601 ctgggactcg gtgcgggtca agctggcagg cccggtcggc gacgccctcc tgcacgccat
     3661 ctaccaggcc gccgacggcc tggcctaccc gagcctgtac gagggcttcg gcctgcccgt
     3721 gctggaggcc atggccaacg ggaccccggt gctgaccagc gaccgctcca gcctgcccga
     3781 ggtcgccggc gacgccgccc tgctggtcga cccgctcgac cggtcggcca tcgccgccgg
     3841 cctggtccgg ctggccggcg acgaggacct gcgccggcgc ctggtcgagg ccggccgccg
     3901 ccgcgccgcc ggcttcacct ggcgggccac cgccgccgcc acctgggcca cctaccgcga
     3961 ggtcgcggag cgccggccgt gagccagaag gatccccgga gc
//
LOCUS       DASSWR010000708         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1185793, whole genome shotgun sequence.
ACCESSION   DASSWR010000708 DASSWR010000000
VERSION     DASSWR010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185793"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFO47_11895"
     CDS             complement(<1..675)
                     /locus_tag="VFO47_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8789438.1"
                     /translation="MTFDVVVPTVGRDSLRRLLTALAEGTGPAPGRVLVVDDRGDRTG
                     PLPLPEGGGIEVEVVPGRAAGPAAARNLGWRRATAGWVAFLDDDVVPEPGWSEALARD
                     LEGLPGNVAASQGRVRVPMPAGRRPTDWERNVRGLETARWATADMAYRREVLARVGGF
                     DERFPRAYREDADLGLRVVRAGYRIVSGRREVTHPVRAAGRFVSVRLQAGNGDDVLML
                     ALHGRGW"
     gene            complement(672..>2065)
                     /locus_tag="VFO47_11900"
     CDS             complement(672..>2065)
                     /locus_tag="VFO47_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET8789439.1"
                     /translation="GPDVTGDGWEGLRTLYAERAPMFLRTALPGLDPASVRYVPHHVA
                     HAASAHLAAPYRSSSVLVLDGRGEAASHLAGRSVDGDLEILAAQRLPHSLGLLYEEVT
                     DHLGFRRSSDEYKVMALAAYGKPAYLRELREAVRVTGDGGFTVAPIELDTLAKRRAKH
                     EEWTGEHADLAASVQLRLQEVLLELVRWLHAQTGDRDLTMAGGVALNCVANSVLAEQG
                     PFERIWVQPAAGDAGTALGAAMHVAHALGDRVAPMPTAALGRGWTDDELAAWLATARV
                     AYERPPDLADAVAEVIAADGVVAWFQGRSEYGPRALGHRSLLANATRAANLERLNDVK
                     GREQFRPVAPMVLADRAGDIFEGPMPSPYMLFTHRVRPAWGERIPAVVHVDGSARIQT
                     VDRDEEPLVARLLEAVERRTGVPVVVNTSLNTAGRPMVDDPRDALECFGSAPVDVLAL
                     GPFLVRRAAFTRP"
BASE COUNT          250 a          794 c          789 g          232 t
ORIGIN      
        1 ccacccccgc ccatgcaagg ccagcatgag cacgtcgtcg ccgttgcccg cctgcagccg
       61 gacgctgacg aaccgccccg ccgcccggac cggatgagtc acctcccgcc gaccggagac
      121 gatccggtac ccggcccgga ccacccgcag ccccaggtcg gcgtcctccc ggtaggcccg
      181 cgggaaccgc tcgtcgaacc cgccgacgcg ggcgagcacc tcccgccggt aggccatgtc
      241 ggccgtggcc cagcgggccg tctccagccc ccggacgttc cgctcccagt cggtcggccg
      301 ccgcccggcc ggcatgggga cccgcacccg cccctggctc gcggccacgt tcccgggcag
      361 gccttccagg tcgcgggcca gcgcctcgga ccacccgggc tccgggacca cgtcgtcgtc
      421 caggaacgcc acccaccccg ccgtcgcccg ccgccagccg aggttgcgcg ccgccgccgg
      481 cccagccgcc cggcccggca ccacctcgac ctcaatcccg ccaccctcgg ggagcggcag
      541 tggccccgtc cggtcgcccc ggtcgtcgac gaccagcacc cgtcccggcg ccggcccggt
      601 cccctcggcc agggccgtca gcagccgccg gaggctgtcc cgccccaccg tgggaacgac
      661 cacgtcgaag gtcatggccg ggtgaaggcg gcccgccgga ccaggaacgg cccgagggcg
      721 aggacgtcga ccggggccga cccgaagcac tcgagggcgt cgcgggggtc gtcgaccatg
      781 ggccggccgg ccgtgttgag gctggtgttg accaccaccg gcaccccggt gcggcgctcg
      841 accgcctcca gcagccgggc gaccagcggc tcctcgtcgc ggtcgacggt ctggatgcgg
      901 gcgctgccgt cgacgtggac cacggccggg atgcgctcgc cccaggccgg ccgcacccgg
      961 tgggtgaaca gcatgtaggg gctgggcatg gggccctcga agatgtcgcc ggcccggtcg
     1021 gccaggacca tgggcgccac cgggcggaac tgctcgcggc ccttgacgtc gttgagccgc
     1081 tccaggttgg ccgccctggt ggcgttggcg agcaggctgc ggtgcccgag ggcgcgcggc
     1141 ccgtactcgc tgcggccctg gaaccaggcc accaccccgt cggcggcgat cacctcggcc
     1201 acggcgtcgg ccaggtcggg cgggcgctcg taggcgaccc gggccgtggc cagccaggcg
     1261 gccagctcgt cgtcggtcca gccccgcccg agggcggcgg tgggcatggg cgcgacccgg
     1321 tcgccgaggg cgtgggcgac gtgcatggcg gcgccgaggg cggtgccggc gtcgccggcg
     1381 gccggctgga cccagatgcg ctcgaacggc ccctgctcgg ccaggaccga gttggccacg
     1441 cagttgagcg cgaccccgcc ggccatggtc aggtcgcggt cgccggtctg ggcgtgcagc
     1501 cagcggacca gctccagcag cacctcctgg aggcggagct ggacgctggc ggccaggtcg
     1561 gcgtgctcgc cggtccactc ctcgtgcttg gcgcgccgct tggccagggt gtcaagctcg
     1621 atcggggcca cggtgaagcc gccgtcgccg gtgacccgga ccgcctcgcg cagctcccgg
     1681 aggtaggcgg gcttcccgta ggcggccagg gccatgacct tgtactcgtc ggaggagcgc
     1741 cggaacccga ggtggtcggt gacctcctcg tagagcaggc cgagggagtg ggggaggcgc
     1801 tgggcggcca ggatctcgag gtcgccgtcg accgagcgcc cggccaggtg ggaggcggcc
     1861 tcgccgcggc cgtcgagcac gagcacgctc gagctgcggt agggggcggc caggtgggcc
     1921 gatgcggcgt gggccacgtg gtgggggacg tagcggacac tcgccgggtc gaggccgggc
     1981 agggcggtgc gcaggaacat cggggcccgc tcggcgtaga gggtgcgcaa cccctcccag
     2041 ccgtcgccgg tcacgtcggg accgg
//
LOCUS       DASSWR010000709         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_394489,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000709 DASSWR010000000
VERSION     DASSWR010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394489"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFO47_11905"
     CDS             complement(<1..355)
                     /locus_tag="VFO47_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8789440.1"
                     /translation="MAEGGRTRRREPPEVRREQLLDAAAQVFLARGLAQATIADVAEA
                     AGLAKGTVYLYFDSKSALLTALRARYTSQLLARSGRLDAPPGRGGYRQQLRSFLGEMY
                     DFCASNQRLHHMLFHA"
     gene            543..2201
                     /locus_tag="VFO47_11910"
     CDS             543..2201
                     /locus_tag="VFO47_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8789441.1"
                     /translation="MSKPTILTVEDDPQVSAAITRDLAGYYGADYRVVGATSGPEALS
                     VLTRLALRDEPVALIAADQRMPHMTGIELLQQARAHAPGSKFLLLTAYADTDVAIKAI
                     NDIGLDYYLLKPWDPPEDRLYPVVDDLLDDWRQAHPDHTSEVRVVGHRWSERGHEVKL
                     FLARNHVPYRWYDVERDPEALRLLDLARAGPADLPLVLVPEGDTLRSPSALELAGALG
                     LRTSAQQRLYDVCIVGGGPAGLGAAVYAASEGLSTVVVEREAPGGQAGQSAAIENYLG
                     FPKGLSGADLTRRAIAQAARFGAEMVLARDVVGFEARGPVRAVRLDGSGEIEARALVV
                     ATGVSYRRLEAEGLDRLTGRGVYYGVNAGEASQTQGDEVYVVGAANSAGQAALHLSRF
                     ARRVVLVVRAATLDGTMSRYLVERITSTPNVAIRYRSQVVSGRGDGHLEALTLADRDT
                     GATEEVPSSWLFVFIGASPRTRWLGPDVVRDDKGFVVTGHDLLAPEWARRWPLPRAPF
                     VLETSVPGVFAAGDVRLDSMKRVASAVGEGAMSVYLVHRYLATV"
BASE COUNT          292 a          838 c          826 g          306 t
ORIGIN      
        1 cggcgtggaa gagcatatgg tggagccgct ggttgctggc gcagaagtcg tacatctcgc
       61 ccaggaacga tcgcagctgc tgccggtacc cgccgcggcc ggggggtgcg tcgaggcggc
      121 cggaccgggc cagcaactgg ctggtgtagc gggcccgcag ggccgtcagc agcgccgact
      181 tggagtcgaa gtagaggtag acggtgccct tggccaggcc ggcggcctcg gccacgtcgg
      241 cgatggtggc ctgggccagg ccgcgggcca ggaacacctg ggcggccgcg tccaggagct
      301 gctcgcggcg gacctccggc ggttcccgcc gcctcgtcct ccccccctcg gccacggcac
      361 ctcccagctg cctgctgact gaccgtcatt ctatggtcac ttccagcggc ggcgcaggcc
      421 gaggaggttg aagacccggc gacgcttcac cgagacgtag aggcgcctcc gcgccggcag
      481 accggcgacc ggtacggcgg ttcctgaagc cgtacccgtc ccggttgcta tgctcctagc
      541 gcatgagcaa gccgacgatc ctgacggtcg aggacgaccc ccaggtgtcc gcggcgatca
      601 cccgcgacct ggccgggtac tacggcgccg actaccgcgt cgtgggcgcg acctcggggc
      661 cggaggcgct gtccgtgctg acccggctcg ccctccgcga cgagccggtg gcgctgatcg
      721 ccgccgacca gcggatgccc cacatgaccg ggatcgagct gctccagcag gcgcgggccc
      781 acgccccggg ctcgaagttc ctgctgctca cggcgtacgc cgacaccgac gtggccatca
      841 aggcgatcaa cgacatcggc ctcgactact acctgctcaa gccgtgggac cctccggagg
      901 accggctgta cccggtcgtc gacgacctgc tggacgactg gcggcaggct caccccgacc
      961 acacctccga ggtgcgggtg gtggggcacc gctggtcgga gcggggccac gaggtcaagc
     1021 tgttcctggc ccgcaaccac gtgccctacc gctggtacga cgtcgagcgc gacccggagg
     1081 cgctgcggct gctcgacctc gcccgggcgg ggccggccga cctcccgctg gtgctcgtcc
     1141 ccgagggcga cacgctgcgc tcgccgtcgg ccctcgagct ggccggcgcg ctcggcctgc
     1201 gcacatccgc ccagcagcgc ctctacgacg tctgcatcgt cggcggcggg ccggccggcc
     1261 tgggcgccgc cgtgtacgcg gcgtcggagg gcctgagcac cgtggtcgtg gagcgcgagg
     1321 cccccggagg gcaggcggga cagagcgcgg ccatcgagaa ctacctgggg ttccccaagg
     1381 gactgagcgg cgccgacctc acccggcggg ccatcgccca ggcggcccgg ttcggcgccg
     1441 agatggtcct ggcccgcgac gtcgtcggct tcgaggcccg cgggccggtg cgggccgtgc
     1501 gcctcgacgg ctcgggcgag atcgaggcgc gcgcgctcgt cgtggccacc ggcgtctcct
     1561 accggcggct ggaggcggaa gggctcgacc ggctcaccgg ccgcggcgtc tactacggcg
     1621 ttaacgccgg cgaggccagc cagacccagg gcgacgaggt ctacgtcgtc ggggcggcca
     1681 actcggccgg ccaggccgcg ctccacctgt cccgcttcgc caggcgggtg gtgctggtcg
     1741 tccgggccgc gaccctcgac ggcacgatgt cgaggtacct ggtggagcgg atcacctcca
     1801 cgcccaacgt cgccatcagg taccgcagcc aggtggtgtc gggccggggc gacggccacc
     1861 tggaggcgct gacgctcgcc gaccgggaca ccggcgccac cgaggaggtg ccgtcgagct
     1921 ggctgttcgt cttcatcggc gcctcgcccc gcacccggtg gctcggcccc gacgtcgtcc
     1981 gcgacgacaa ggggttcgtc gtcaccggcc acgacctgct ggctcccgaa tgggcgcggc
     2041 ggtggccgct cccacgggcc ccgttcgtgc tggagacgag cgtgcccggc gtgttcgcgg
     2101 ccggcgacgt ccggctcgac tcgatgaagc gggtggcgtc ggccgttggc gagggcgcca
     2161 tgtccgtcta cctcgtgcac cgctacctgg ccaccgtctg atgcgcgccg acgagctccg
     2221 cccgctgtcc atcttcgacg gcctggccga cgagcagcca cc
//
LOCUS       DASSWR010000710         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_252665,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000710 DASSWR010000000
VERSION     DASSWR010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252665"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..886
                     /locus_tag="VFO47_11915"
     CDS             74..886
                     /locus_tag="VFO47_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8789442.1"
                     /translation="MPTLEPAQIALLAGAALLAGAVNAVAGGGSLLSFPALLAVGYPA
                     LTANVTNTVALTPGYFGGTLGYRRELEGQRGRALALGAISAVGAVLGAWLLLISSAEL
                     FERVVPFLIFLACGLLAAQPRLARLVRERREAPLPAADPPLAGARADAGDHHTLPLFG
                     AQFLAAAYGAYFGAGVGIMMLAILGIFVADNLQRLNALKGLLSLLINLIAAVSFVLFA
                     DVSWVAVAVMAVASLLGGQLGVVLARRLNDRALRWLVIAFGVAVGLRLLLAG"
     gene            complement(883..1359)
                     /locus_tag="VFO47_11920"
     CDS             complement(883..1359)
                     /locus_tag="VFO47_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8789443.1"
                     /translation="MLEKLLLAVDQSENSRKAVPAVIELARAGGGTVHVLHVRELYYP
                     VPPTVRGDSAEEAQELVDGIVKELQAAGVTVEGAVRPSSGGSPAGEVLNHAREVDAGM
                     IVVGSHGRSPLGGLLLGSVANKLTQLSTCPVLVVRDQESLEERHHDVLRAKTRFQS"
     gene            1508..2035
                     /locus_tag="VFO47_11925"
     CDS             1508..2035
                     /locus_tag="VFO47_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HET8789444.1"
                     /translation="MRALVVYESMFGNTQTIADAIAGGLAQRMRVDAVEVGSAPATLD
                     DDVTLLVVGGPTHAFGMSRPRTRQDAATQTPDGLVSTGIGLREWLAALRAPADMAAAA
                     FDTKVNKPHLPGSAARAAEKRLRRLGFRIAAPAESFYVGGTLGPLEEGEQDRAMRWGD
                     QLGATTREPAGGARP"
BASE COUNT          266 a          817 c          774 g          301 t
ORIGIN      
        1 gacccagccc cgcgggtcct tcgacggcgc agggcgccaa gcaggctcga cgattgacta
       61 tcgtacgagg cccatgccga ccctcgaacc cgcccagatc gcgctcctcg ccggggcggc
      121 cctgctcgcc ggcgccgtca acgcggtcgc cggcggcggg tcgctgctgt cgttcccggc
      181 cctgctcgcg gtcggctacc cggcgctgac ggccaacgtc accaacacgg tcgcgctcac
      241 ccccggctac ttcgggggga ccctcggcta ccggcgcgag ctcgagggcc agcggggccg
      301 ggccctcgcc cttggtgcca tcagcgccgt cggggccgtg ctgggcgcct ggctgctgct
      361 gatcagctcg gccgagctgt tcgagcgcgt cgtgccgttc ctgatcttcc tggcctgcgg
      421 gctgctcgcc gcccagcccc gcctggcccg gctggtccgg gagcgccgcg aggccccgct
      481 gcctgcagcc gacccgccac tcgccggcgc ccgggccgac gccggagacc atcacacgct
      541 gccgctgttc ggcgcccagt tcctcgccgc cgcctacggc gcctacttcg gcgccggggt
      601 cgggatcatg atgctggcca tcctgggcat cttcgtcgcc gacaacctgc agcggctgaa
      661 cgccctcaag gggctgctgt cgctgctgat caacctgatc gccgccgtct cgttcgtgct
      721 cttcgccgac gtcagctggg tcgcggtggc ggtgatggcg gtggccagcc tgctcggagg
      781 ccagctcggg gtcgtcctgg cccggcgcct caacgaccgg gccctgcgct ggctggtgat
      841 cgccttcggg gtcgccgtcg gcctgcgcct gctgctggcc ggctaggact ggaaccgggt
      901 cttggcgcgg aggacgtcgt ggtggcgctc ctccaggctc tcctggtcgc gcacgaccag
      961 gaccgggcag gtcgagagct gggtcagctt gttggcgacg ctgccgagca gcagcccgcc
     1021 cagcggcgag cggccgtggg agccgaccac gatcatgccg gcgtcgacct cgcgggcgtg
     1081 gttgagcacc tccccggccg gtgagccacc gctgctcggt cggacggcgc cctcgaccgt
     1141 caccccggcc gcctgcagct ccttgacgat gccgtcgacc agctcctggg cctcctcggc
     1201 gctgtcgcct cggacggtcg gcggcacggg gtagtagagc tcgcggacgt gcaggacgtg
     1261 gacggtccca ccgccggcac gggccagctc gatcacggcc gggacggcct tgcggctgtt
     1321 ctccgactgg tccacggcca ggagcagctt ctcgagcatc gggcacctcc agaattcggc
     1381 ggatagatgg cttcccgccc ggcaccgccc gaaaacccga acggcgcggg acgttcgcct
     1441 ctgtcccggc accggggccg gtggtgtgat cgagcaggag gcacccatcg gcgcgaggag
     1501 gaacgagatg cgcgcgctcg tggtctacga atcgatgttc ggcaacaccc agacgatcgc
     1561 cgacgccatc gccggtgggc tggcccagcg gatgcgggtc gacgccgtgg aggtcggcag
     1621 cgccccggcg acgctcgacg acgacgtcac gctgctcgtc gttggcggtc cgacccacgc
     1681 cttcgggatg agccggccga ggacacgcca ggacgccgcc acccagaccc ccgacgggct
     1741 ggtgtccacc gggatcgggc tgcgggagtg gctcgccgcc ctgcgggcgc cagcggacat
     1801 ggccgcggcc gcgttcgaca cgaaggtgaa caagccccac ctgccggggt cggccgcccg
     1861 ggccgcggag aagcggctgc gccgcctcgg cttccgcatc gccgccccgg cggagagctt
     1921 ctacgtcgga ggaaccctcg gccccctcga ggagggcgag caggaccgcg ccatgcgctg
     1981 gggcgaccag ctcggcgcca ccacccgcga gcctgcgggc ggcgcccgac cctgaccggc
     2041 caaggcgggg aggtgtccgg ccgggccagc cggcccggga acccgcctct gagctaacct
     2101 cgagcaccac ccgagtcgcg cgtgggacag tcgtgaccga ggccattgag ctgcccgc
//
LOCUS       DASSWR010000711         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_902941,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000711 DASSWR010000000
VERSION     DASSWR010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902941"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1426)
                     /gene="ahcY"
                     /locus_tag="VFO47_11930"
     CDS             complement(173..1426)
                     /gene="ahcY"
                     /locus_tag="VFO47_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET8789445.1"
                     /translation="MPYDVTDLALADEGRLRIEWADRHMPVLRDIRARFEKERPLEGL
                     KVAACLHVTTETANLMRTLKAGGASVVLCASNPLSTQDDTAAALVEHYGIDTFARRGV
                     DTDGYYAHLEAALDTRPNITMDDGCDLVTLLHTRRRDQLAEVRAGTEETTTGVIRLRQ
                     MEREGALAYPIIAVNDTPTKHLFDNRYGTGQSTIDGILRATNLLLAGTTFVVAGFGYC
                     GKGLTMRARGLGAKVIVTEVDPIRALEAAMEGFKVAPMAQAAREADVIVSVTGDRDVM
                     RREHFEVMKDGVVLANSGHFDVEIDKAALADLATSVVRVRENVEEFTLEDGRRLNLVA
                     EGRLVNLGAAEGHPAAVMDMSFADQSLSLEWLSRQQDLEVRVHDVPAEIDAEVARVKL
                     ATMNVEIDTLTDTQERYLHSWTDGT"
     gene            complement(1466..2326)
                     /locus_tag="VFO47_11935"
     CDS             complement(1466..2326)
                     /locus_tag="VFO47_11935"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET8789446.1"
                     /translation="MSDTPEEVAAGPAGEQPHRPSVPEAVRVAAAALEPRLSERPVVA
                     LVLGSGLGGLADEVEDATRVPAAEVPGMPVPKVPGHAGAVVAGRLAGVPVLVLAGRVH
                     TYEGYSAAEVAFATRVAAEVGCRALVATNAAGGLNPDYDPADLMVLTDHISFLFDNPL
                     RGAPDFVDLAGAYDPELRSAAVEAGQAKGVPVREGVYLAAAGPSYETPAEIAMFRAWG
                     ADAVGMSTVPEVIAARAHGLRVAAISAITNVHRPGGTPTSHAEVLEVAALVRPRFREL
                     VLSLLPVAAA"
     gene            complement(2323..3417)
                     /locus_tag="VFO47_11940"
     CDS             complement(2323..3417)
                     /locus_tag="VFO47_11940"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HET8789447.1"
                     /translation="MSAATGDHSGPSVLDDPAELARRDPGAMLAEVAGAGGQARVALR
                     AAQAAPLVGPLPEVVVVAGMGGSGIAGDVLSAMAFPESPVPVLAVKGDRLPAFVGPGT
                     LLVAVSYSGNTAETLSAVEQGLAAGARLVAVTSGGALAELAQSRGAPLVAIEGGRMPR
                     AALWSLLVPVCLAAEGAGVLPPVTDQVCAAADVLDEEAAALAPEVATAANPAKQAAVA
                     LLDRLPVVWGSGQVGAVAATRFRTQCNENAKVSVVSGPLPEVNHNDVMGLEGGLGPDR
                     ELVLLRDEAGEHDRDGRRIEAVLAALGVADPVGHDAGPGPALARLARLTAFADFTSTY
                     LGVARGVDPTPIRTLDEVKAALSAEARAPR"
     gene            complement(3414..3599)
                     /locus_tag="VFO47_11945"
     CDS             complement(3414..3599)
                     /locus_tag="VFO47_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET8789448.1"
                     /translation="MPLDAKLLEILACPNCHGEVEYKEAEQVIECRGECRYRYPVRDD
                     IPVMLIEEAEKPGTAPA"
     gene            complement(3624..4982)
                     /locus_tag="VFO47_11950"
     CDS             complement(3624..4982)
                     /locus_tag="VFO47_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET8789449.1"
                     /translation="MPAALDQVFKAYDVRGVYPDQLDEELAEGIGRAFVALTGTGTLA
                     IGQDMRPSSKPMADAVAAGAMAQGADVLRVGLCSTDMLYYVSGALDVAGVMLTASHNP
                     ARYNGLKLCRAGAVPVGEESGLADIKRMIAAGEPATPSAHRGRERQADDLLERYAAHV
                     RSFADPSVLRPLKVVADAGNGMAGHVVPAVFRGLPFELVAMYFELDGSFPNHPADPLN
                     PDNLRELQARVVAEGADVGLAFDGDADRVFLVDELGATVPSSLVGALVARLMCEREPG
                     ATVLYNLICSRVVPETIREAGGVPVRTRVGHSFIKGVMAETGAVFGCEHSGHYYFRDN
                     YRADSGLIAALVALDALSRAGRPLSEVLAPYRRYAASGEVNRRVAGDPRQVIQAVAGR
                     YPADRADFADGLTVEGGDWWFNLRPSNTEPLLRLNVEAATEAEMARVRDEVLEAVGGE
                     PA"
BASE COUNT          648 a         1930 c         2041 g          682 t
ORIGIN      
        1 gggccggtgg gtcggccgca gggcccgggc ctgaaggcgg ttctgggggg ctgtgggcaa
       61 ccgccggcgc ccctcccgcg ggtcggcgag gctcccccac cgggggggcc gagtcggcgg
      121 ctccggcgcc ggcgcctgga cccgcgccgg cggccgggtt cgctccgcct ggttaggtgc
      181 cgtcggtcca ggagtggagg tacctctcct gggtgtcggt cagggtgtcg atctcgacgt
      241 tcatggtggc cagcttgacc ctggccacct cggcgtcgat ctcggccggg acgtcgtgga
      301 cccggacctc caggtcctgc tggcgcgaca gccactccag cgacagcgac tggtcggcga
      361 acgacatgtc catgaccgcg gccgggtggc cctcggcggc gcccaggttg accaggcggc
      421 cctcggcgac caggttgagg cggcggccgt cctccagggt gaactcctcg acgttctcgc
      481 ggacccggac cacggaggtg gccaggtcgg ccagggcggc cttgtcgatc tcgacgtcga
      541 agtggccgga gttggccagc acgaccccgt ccttcatgac ctcgaagtgc tcgcgccgca
      601 tgacgtcgcg gtcaccggtg accgacacga tcacgtcggc ctcgcgggcc gcctgggcca
      661 tgggcgcgac cttgaagccc tccatggccg cctcgagggc gcggatcggg tcgacctcgg
      721 tgacgatgac cttggccccg aggccgcggg cgcgcatggt caggcccttg ccgcagtagc
      781 cgaagccggc gaccacgaag gtcgtgccgg ccaggagcag gttggtggcc cgcaggatcc
      841 cgtcgatggt cgactggccg gtgccgtagc ggttgtcgaa caggtgcttg gtcggggtgt
      901 cgttgacggc gatgatcggg taggcgagcg cgccctcccg ctccatctgc cgcagccgga
      961 tgaccccggt ggtggtctcc tcggtgccgg cccggacctc ggccagctgg tcgcgccgcc
     1021 tggtgtgcag cagggtgacc aggtcgcagc cgtcgtccat ggtgatgttg gggcgggtgt
     1081 cgagggccgc ctccaggtgg gcgtagtagc cgtcggtgtc gaccccgcgg cgggcgaagg
     1141 tgtcgatgcc gtagtgctcg accagcgcgg ccgcggtgtc gtcctgggtc gacagcgggt
     1201 tggaggcgca cagcaccacg ctggcgccgc cggccttgag ggtgcgcatc aggttggcgg
     1261 tctcggtggt cacgtgcagg caggccgcga ccttgaggcc ttcgaggggc cgctccttct
     1321 cgaagcgggc gcggatgtcg cgcagcaccg gcatgtgccg gtcggcccac tcgatgcgca
     1381 ggcgcccctc gtcggccagg gcgaggtcgg tcacgtcgta cggcaaggat cctcctccgg
     1441 ttgggtgccg cgtaaggcta ccggatcagg cggcggccac aggcagcagg gacagcacga
     1501 gctcgcggaa ccggggccgg accagggccg cgacctcgag gacctcggcg tggctggtcg
     1561 gggtgccgcc gggccggtgc acgttggtga tggccgagat ggcggccacc cgcaggccgt
     1621 gggcgcgggc ggcgatcacc tcgggcacgg tcgacatgcc gaccgcgtcg gcgccccagg
     1681 cgcggaacat ggcgatctcg gccggggtct cgtaggaggg cccggcggcg gccaggtaca
     1741 cgccctcgcg gaccgggacg cccttggcct ggccggcctc gaccgccgcc gatcgaagtt
     1801 ccgggtcgta ggcgccggcc aggtcgacga agtcgggggc gccgcgcagc gggttgtcga
     1861 acaggaagct gatgtggtcg gtgaggacca tcaggtcggc cgggtcgtag tcggggttga
     1921 ggccgccggc ggcgttggtc gccaccaggg cgcggcagcc gacctcggcc gcgacccggg
     1981 tcgcgaacgc gacctcggcc gcgctgtagc cctcgtaggt gtggacccgg ccggccagga
     2041 cgagcacggg cacgccggcc aggcggcccg cgaccaccgc cccggcgtgc ccggggacct
     2101 tgggcacggg catgccgggc acctcggccg ccgggacccg ggtggcgtcc tcgacctcgt
     2161 cggccagccc gcccagcccc gaccccagca ccagcgccac caccggccgc tcagagagcc
     2221 gaggttccag cgccgccgcc gccacccgca ccgcctccgg caccgacggc cggtgcggct
     2281 gctcccccgc cggcccggcc gccacctcct ccggggtgtc gctcaccggg gggctctggc
     2341 ctcggcggag agggccgcct tgacctcgtc gagggtgcgg atcggggtgg ggtcgacccc
     2401 gcgggcgacg cccaggtagg tggaggtgaa gtcggcgaag gcggtcaggc gggccaggcg
     2461 ggccagggcc gggcccggac cggcgtcgtg gccgaccggg tcggcgaccc ccagggcggc
     2521 gaggacggcc tcgatgcggc ggccgtcgcg gtcgtgctcg ccggcctcgt cgcgcagcag
     2581 gaccagctcg cgatccgggc cgaggccgcc ctccaggccc atgacgtcgt tgtggttgac
     2641 ctccggcagg ggcccggaca cgaccgagac cttggcgttc tcgttgcact gggtgcggaa
     2701 ccgggtggcc gcgaccgccc cgacctggcc gctgccccag acgacgggca gccggtccag
     2761 cagcgccacg gcggcctgct tggccgggtt ggcggccgtg gccacctccg gggcgagggc
     2821 ggccgcctcc tcgtcgagga cgtcggcggc ggcgcagacc tggtcggtca cgggcggcag
     2881 caccccagcc ccctcggccg ccaggcagac cgggaccagc agcgaccaca gggccgcccg
     2941 gggcatgcgg ccgccctcga tcgcgaccag gggcgccccg cgggactggg ccagctcggc
     3001 cagggcgccg cccgaggtga ccgcgaccag gcgggccccg gcggccagcc cctgctcgac
     3061 cgcggacagg gtctcggcgg tgttgcccga gtaggagacg gccaccagca gcgtgcccgg
     3121 ccccacgaac gccggcagcc ggtcgccctt gacggccagc acgggcacgg gcgactccgg
     3181 gaaggccatc gcgctcagca cgtcgccggc gatcccggag ccgcccatgc cggccacgac
     3241 cacgacctcg ggcaggggcc cgaccagcgg cgccgcctgg gcggcgcgca gggcgacccg
     3301 cgcctgcccg ccggcgccgg cgacctcggc gagcatggcg cccgggtcgc gccgggccag
     3361 ctcggccggg tcgtcgagca cggagggccc ggagtggtcg cccgtcgccg cgctcacgcc
     3421 ggcgccgttc ccggcttctc ggcctcctcg atcagcatga ccgggatgtc gtcgcgcacc
     3481 gggtagcggt agcggcactc cccccggcac tcgatgacct gctcggcctc cttgtactcg
     3541 acctcgccgt ggcagttcgg gcaggcgagg atctccagca gcttggcgtc aaggggcatc
     3601 gtcggttcct ctcctcgggc cgttcaggcc ggctcgccgc cgaccgcctc cagcacctcg
     3661 tcgcgcaccc tggccatctc ggcctcggtg gcggcctcga cgttgagccg gagcagcggc
     3721 tcggtgttgc tcggtcgcag attgaaccac caatcgccgc cctccacggt cagcccgtcg
     3781 gcgaagtcgg cccggtccgc cgggtaccgc ccggccacgg cctggatgac ctgccggggg
     3841 tcgccggcga cgcggcggtt gacctctccc gaggcggcgt agcgacggta gggggcgagg
     3901 acctcggaca ggggccgccc ggcccgcgag agggcgtcaa gggcgaccag ggcggcgatc
     3961 agccccgagt cggcccggta gttgtcgcgg aagtagtagt ggcccgagtg ctcgcagccg
     4021 aagacggccc cggtctcggc catgaccccc ttgatgaagc tgtggccgac ccgggtgcgc
     4081 accggcacgc cgccggcctc gcggatcgtc tcggggacga cccgggagca gatgaggttg
     4141 tagaggacgg tcgcgcccgg ctcgcgctcg cacatgaggc gggccaccag ggccccgacc
     4201 aggctggagg ggacggtcgc gcccagctcg tcgaccagga acacccggtc ggcgtcgccg
     4261 tcgaaggcca gcccgacgtc ggccccctcg gccaccaccc gggcctggag ctcccggagg
     4321 ttgtcggggt tgagcgggtc ggccgggtgg ttggggaagc tgccgtcgag ctcgaagtac
     4381 atcgccacca gctcgaacgg caggccccgg aagacggccg ggacgacgtg gccggccatg
     4441 ccgttgccgg cgtcggcgac caccttgagc ggtcgcagga cggaggggtc ggcgaagctc
     4501 ctgacgtggg cggcgtagcg ttccagcagg tcgtcggcct gtcgttcgcg cccgcggtga
     4561 gcgctcgggg tggccggctc gcccgcggcg atcatccgct tgatgtcggc caggcccgac
     4621 tcctccccca ccggcaccgc gccggcccgg cacagcttga ggccgttgta gcgggccggg
     4681 ttgtgggagg cggtcagcat gaccccggcg acgtcgaggg cgccggagac gtagtagagc
     4741 atgtcggtcg agcagagccc gacccggagc acgtcggccc cctgggccat cgccccggcg
     4801 gccaccgcgt cggccatcgg cttggacgac ggccgcatgt cctgcccgat ggccagggtc
     4861 ccggtcccgg tcagggccac gaacgcccgc ccgatcccct cggccagctc ctcgtccagc
     4921 tggtccgggt agaccccgcg gacgtcgtag gccttgaaca cctggtcgag ggcggcgggc
     4981 acggcagctc cttgcggcgg gtccggcgga ggctggggac gcccgcgatt ctagcccccg
     5041 ccggtcacgg cccgccgacc cgccccgggc tcagaaccgg cgttcgccgg ccagcgagag
     5101 gaccgggggt ggctcgcagg ccacccccgg acgggctagc acgttcgcga cgcccgcggt
     5161 aactgtcagg tttcccctac cggttcccgt gagcgccggg gggaaggtac gacggcccgg
     5221 gctgccggtc aagcatgtcg gcgttaaggt ttgggcggcc acgttcgcct cccggaagga
     5281 gggcctcctg ccccgtcccc g
//
LOCUS       DASSWR010000712         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1072782, whole genome shotgun sequence.
ACCESSION   DASSWR010000712 DASSWR010000000
VERSION     DASSWR010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072782"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFO47_11955"
     CDS             complement(<1..621)
                     /locus_tag="VFO47_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789450.1"
                     /translation="MSGPAPGADAPAQPAGRRYQQQLARRLKVAGNMTITLAVIGPAA
                     SVFAIGSVALRQQGSGAFLAFLLAALISGCLAVAWAELGVLYPTAGGVYGIVARVLGR
                     RAGFLALTLQLALFVIVPSAFALAAGQSVAALWPAISPRTGALVLLAAATALAVVGIR
                     FNAAVTATFLALELAIILVVSVLGFANADWSLAGTLLDPRVYAADGS"
     gene            complement(798..>2527)
                     /locus_tag="VFO47_11960"
     CDS             complement(798..>2527)
                     /locus_tag="VFO47_11960"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET8789451.1"
                     /translation="EIRVADLQFTDEEAVALLNGSMRLQLATEDVARLAERTEGWAAG
                     LVLAGLSLRGRQDPSAFITAFHGDNRHVADYLSAEVFERQSGEIRRFLLRTSILERLS
                     GPLCDAVLEAEGSADLLRELERSNLFLVPLDDHREWYRYHHLFAELLRLELASREPAL
                     LATLHRRAAAWHRQAGNLDEAIGHASAAGEFAQAAALIAQHWLSYWRRGQRATVARWL
                     DGLPDEAILADPPVAYVAAWIRGYSGASKQQIEDWLAVVGGDGAEGVLPDGVGSEGGL
                     PDGVSSLAFGANLARASLVFDDLGRSAAAGRRALELAGPDSLQFWWMAQSALGHALYL
                     SGQAAETRPQLEELVGRVPAAAQPVAVVLALAVLSLLAGDQNDDRTAMALARQAAATA
                     DNQGLSAEPMCGIAYAALGRALARQGELAEAEEQLERALAPVGIDSMLGQRAFALLLL
                     APVRRGCGDLPGARALVEEARELIERFADPGVLPALLNQTEQALVTAPRRRPVVEPLT
                     ERELAVLRLLATRLSNREIGRQLYVSVNTVRTHLQAVYRKLGVGTRADAVAHARELGL
                     LPGSMPPSP"
BASE COUNT          378 a          926 c          916 g          307 t
ORIGIN      
        1 gctaccgtcg gcggcataca cgcgcggatc cagcagcgtg cccgccaacg accagtcggc
       61 gttggcgaaa ccgagaaccg agacgaccag gatgatggcc agctccaggg cgaggaaagt
      121 ggcggtgacg gcggcgttga accggatccc gaccaccgcc agcgcggtgg cggcggccag
      181 caggaccagc gcgcccgtcc gggggctgat ggctggccac agggcggcca cggattggcc
      241 ggcggccagc gcgaacgcgc tggggacgat gacgaaaagg gccagctgca gggtcaacgc
      301 caggaagccg gcccggcggc cgaggacccg ggcgacgatc ccgtacacgc caccggcggt
      361 ggggtacagc acgcccaact cggcccaggc caccgccagg cagccgctga tcagggccgc
      421 gagcaggaag gccaggaagg cgccgctgcc ctgctggcgt aacgccaccg agccgatggc
      481 gaacaccgag gctgccgggc cgatcacggc cagggtgatc gtcatgttgc cggccacctt
      541 cagcctgcgg gcaagctgct gctggtagcg gcggccggct ggctgtgcgg gcgcgtccgc
      601 gcccggtgcc ggaccgctca ccgcagcccc acccgtctga cccagttgac cgcctcacgg
      661 cgaccggtgg cgccaaggta gggcatcggc cgtgcctcga gctgacacag cccttccggg
      721 cgccgatcgt ccggccgtcg acacatgact cggggtgcgg tactcatcga tccgctgcag
      781 tcctccgggc gcaatggcta cgggctgggg ggcattgacc ctgggagcag gccgagctcg
      841 cgggcgtggg cgaccgcgtc ggcccgggtg cccacgccga gcttgcggta gaccgcctgg
      901 aggtgggtcc tgacggtgtt gaccgagaca tacagctggc ggccgatctc ccggttcgac
      961 agccgggtgg cgagcagccg caggaccgcc agctcccgct cggtgagcgg ttcgaccaca
     1021 ggccgccggc ggggcgccgt gaccagcgcc tgctcggtct ggttcaggag cgccggcagc
     1081 acgcccgggt cggcgaaccg ttcgatcagc tcccgggcct cctcgaccag cgccctggcc
     1141 cccgggaggt cgccgcagcc ccggcgcacg ggcgccagca gcaacagcgc gaacgcacgt
     1201 tgcccgagca tactgtcgat cccgaccggc gccagcgccc gctccagctg ctcctcggcc
     1261 tcggccagct cgccctggcg ggccagggcc cggcccagcg ccgcataggc gatcccgcac
     1321 atcggctcgg cgctgagccc ttggttgtcg gcggtcgccg ccgcctgacg ggcgagcgcc
     1381 atggcggtgc ggtcgtcgtt ctggtccccg gcgagcagcg acagcacggc cagcgccagg
     1441 acgaccgcga ccggttgtgc ggcggcgggc acccgcccga ccagctcctc cagctgcggc
     1501 cgcgtctcgg cggcctgtcc ggacaggtac agggcatgac cgagcgcgga ctgcgccatc
     1561 caccagaact gtaaggagtc ggggccggcc agctccagcg cacgccggcc tgcagcggcc
     1621 gagcggccca ggtcgtcgaa caccagcgag gcgcgggcca agttggcgcc gaaggccagg
     1681 gagctgacgc cgtccggcag gcctccctcc gagccgacgc cgtccggcag gactccctca
     1741 gcgccgtcac ctcccacaac ggccagccag tcttcgatct gctgcttgga ggcgccgctg
     1801 tacccacgaa tccaggcggc cacgtaggcg accggcgggt cggccaggat ggcctcatca
     1861 ggcagcccgt ccagccagcg ggccacggtc gcccgttgcc ctcggcgcca gtagctgagc
     1921 cagtgctgcg cgatcagcgc agcggcctgg gcgaactccc cggccgcgct cgcgtgaccg
     1981 atggcctcat ccaggttgcc tgcctgccga tgccaggccg cggcccgccg gtgcagggtc
     2041 gccagcagtg caggctcgcg gctggcgagc tccaggcgca gcagctccgc gaacaggtgg
     2101 tggtagcggt accactcccg gtggtcgtcc agcgggacca ggaacaggtt ggaacgctcc
     2161 agctcgcgca ggaggtcggc tgacccctcg gcctccagga cggcgtcgca tagggggccg
     2221 gacaggcgct ccaggatcga ggtccgcagc aggaacctcc tgatctcccc tgattgacgc
     2281 tcgaacacct cggcggagag gtagtcggcg acatggcggt tgtcgccgtg gaacgcggtg
     2341 atgaaggcgc tcgggtcctg ccggccgcga agcgacagcc cggccaggac cagcccggcc
     2401 gcccagccct cggtccgctc ggccaggcgc gccacgtcct cggtggccag ctgcagccgc
     2461 attgagccgt tcagcagtgc gacggcctct tcgtcggtga actgcagatc ggcgacgcgg
     2521 atctcgg
//
LOCUS       DASSWR010000713         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_649404,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000713 DASSWR010000000
VERSION     DASSWR010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649404"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFO47_11965"
     CDS             <1..444
                     /locus_tag="VFO47_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8789452.1"
                     /translation="NAAIHYDTWQRATTAHLRVVREALEVNLLGAWQASLVLLPLLRA
                     SGHGRIVNVSSEAGSLAGMGGGGAPAYNVSKAALNALTRMLAGDLRRDRVLVNAVCPG
                     WVATDMGGPGGRPVAEGAASVLWAVDLPDDGPTGGFYRDGRPVPW"
     gene            complement(670..1977)
                     /locus_tag="VFO47_11970"
     CDS             complement(670..1977)
                     /locus_tag="VFO47_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789453.1"
                     /translation="MNVEQTTATETQALRELAAFPLVEAIFGRRSRRFALGDELPDGP
                     LAYRSRHEPLPLSDLERMLVLSAMGGTTGWHYSITRHARYAPHISNYAAGAAGRTFPS
                     AAGFHTAELFFTDDTGVYFFPTRDAGALVDPATEEVTAELMVERHRDRARQLSGRRLY
                     LPAEEPYLEGHNTWCVNVPGSLLVIPVADIAQHLLAILCFFTQNGYAIYDDVNRRQIP
                     GLEQFSGLVDVEEPFPLTFAEQYALTEATAELASACYAGVLLLQAMGLGGWMFDGIDR
                     FSMLGASGNPEVPGLGFRYDTDERWSTPNPTGREGVYEAYCPPHHRDMAAAVEAFAQR
                     KFGPGGPFNPDTPGAWTDSPGFRGSAQVHDETFKACVALQAQYVFDTFGKFPGTVPTL
                     FIMNYVQAHHLDLEFYDRFFKPGAYLATHADHMANWHGRPEEG"
BASE COUNT          303 a          728 c          781 g          316 t
ORIGIN      
        1 aacgccgcca tccactacga cacctggcag cgggccacca ccgcccacct ccgggtggtc
       61 agggaggcgc tggaggtcaa cctgctcggc gcctggcagg ccagcctcgt cctgctcccg
      121 ctgctgcggg ccagcggcca cgggcgcatc gtcaacgtct ccagcgaggc ggggtcgctg
      181 gccggcatgg gcggcggggg cgcgcccgcc tacaacgtct ccaaggcggc gctcaacgcc
      241 ctcactcgca tgctcgccgg cgacctgcgg cgggaccggg ttctcgtcaa cgccgtctgc
      301 ccgggctggg tggccaccga catggggggt cccggcgggc ggccggtcgc ggagggggcg
      361 gccagcgtgc tgtgggccgt ggatctgccc gacgacgggc ccaccggtgg cttctaccgc
      421 gacggccgtc cggtcccctg gtgacacccc gccacacctg cgacggtgct gttggagcgc
      481 tccgacgcga tgaacgagct ggacgggacg ctggctggga tcgcgtatga cgtcgcccgc
      541 cagtcccgcg gcgcgtggtc agcccttggg gacgaaggcc tgaacccctt cacggaggct
      601 gcgagggcgc ccatacccgg tcgaaccggg agcaacaccc tagttggcgc gcatatgccg
      661 gcccatcgct cagccctcct ccggtcgacc gtgccagttg gccatgtggt cggcgtgggt
      721 ggccaggtag gcgccgggct tgaagaagcg gtcgtagaac tccaggtcca ggtggtgggc
      781 ctggacatag ttcatgatga acagcgtcgg cacggtgcca gggaacttgc cgaaggtgtc
      841 gaagacgtac tgcgcctgca gggccacgca cgccttgaag gtctcgtcgt ggacctgagc
      901 gctgccgcgg aaccccgggc tgtcggtcca ggcgccgggg gtgtcggggt tgaaggggcc
      961 gcccggcccg aacttgcgct gggcgaaggc ctccaccgcc gcggccatgt cgcgatggtg
     1021 cggcgggcag taggcctcgt agaccccctc gcggccggta gggttgggcg tggaccagcg
     1081 ctcgtcggtg tcgtagcgga accccaggcc ggggacctcg gggttgccgg aggcacccag
     1141 catggagaag cggtcgatcc cgtcgaacat ccagccgccc agccccatcg cctgcaacag
     1201 cagcaccccg gcgtagcagg cgctggccag ctcagcggtg gcctcggtca gggcgtactg
     1261 ctcggcgaag gtcagcggga acggctcctc gacatcgacc aggccgctga actgctccag
     1321 tcccgggatc tggcggcggt tgacgtcgtc gtagatcgcg tacccgttct gggtgaagaa
     1381 gcacaagatg gccagcaggt gctgggcgat gtcggccacg gggatgacca gcagcgagcc
     1441 ggggacgttc acgcaccagg tgttgtggcc ctccagatac ggctcctccg ccggcaggta
     1501 cagccgccgc cccgacagct ggcgggcccg gtcgcggtgg cgctcgacca tcagctcagc
     1561 cgtcacctcc tcggtggccg ggtcgaccag ggcgccggcg tcccgggtgg ggaagaagta
     1621 cacccctgtg tcgtcggtga agaacagctc ggcggtgtga aagccagccg ctgagggaaa
     1681 cgtgcgcccg gctgcgccgg cggcgtagtt ggagatgtgg ggggcgtagc gggcgtgccg
     1741 ggtgatcgag tagtgccagc ccgttgtccc gcccattgcg ctcagcacca gcatccgctc
     1801 caggtcgctg agcggcagcg gctcgtgcct ggagcgatag gcgagcgggc cgtcggggag
     1861 ctcatcgccc agggcgaacc ggcgcgaccg gcggccgaag atcgcctcca ccagcgggaa
     1921 ggcggccagc tcacggagcg cctgggtctc ggtggcggtg gtctgctcaa cgttcatccg
     1981 gtccctcctt gctccggtct caggaggccg tcttccgtcg gctcgatgcc tccatgaggt
     2041 cctatgccga taccgattgc ccttccgcag gagggcgtct cggtcgcggg cgacccgctc
     2101 gaggcgttcc cactgcaccg cctcgccc
//
LOCUS       DASSWR010000714         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1326724, whole genome shotgun sequence.
ACCESSION   DASSWR010000714 DASSWR010000000
VERSION     DASSWR010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326724"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFO47_11975"
     CDS             <1..516
                     /locus_tag="VFO47_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8789454.1"
                     /translation="QHCHRADDIATALRLADEFGYRLVIDHGTEAVLLADRLAERQVP
                     VLIGPLLTSRSKVELRNRSLANPGRLAAAGVELGIITDHPVVPVHLLHVQAALAVREG
                     LDPADALRAVTLTPARVLGLEDRLGSLEPGKDATLCVWSGDPLDARSRVESAWIEGRQ
                     VYGADPDDPPF"
     gene            complement(625..1608)
                     /locus_tag="VFO47_11980"
     CDS             complement(625..1608)
                     /locus_tag="VFO47_11980"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET8789455.1"
                     /translation="MADLITYREAVAAGIAQEMRRDETVVCLGEDIGAAEGVFKTAAG
                     LFAEFGPERVWDTPISEQAIVGAAMGAAMTGLRPVAEIMFSDFLACCWDYVANEIPKM
                     RYMTGGQVTVPLVVRTANGGGLGFGAQHSQAVENWPFAVPGLKIAAPSTPADMVGLMA
                     AAIRSDDPVLVFEHKSLFASKGDPPPSGDHVVPLGAAEVRRPGGDVTLVALASTVPTA
                     MAAAEQLAADGVDTEVIDLRCLIPLDARTVLGSVARTSRLVIVEENPYQGGWGGTVAS
                     IVAEEAFYDLDAPIRRVASACVPLPFAANLEQAVVPTADRVATTVREVIDA"
     gene            complement(1596..2594)
                     /locus_tag="VFO47_11985"
     CDS             complement(1596..2594)
                     /locus_tag="VFO47_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET8789456.1"
                     /translation="MTVVAEEPKVEELLELYERMALIRATEKAAFDLFMAGLVKGTTH
                     LANGQEAVAVGASAALEPDDYVFATYRGHHHAIARGASPEACLAELMSRATGLCGAKG
                     GSMHLTVADHNMLGSYAIVGAHLPIACGAAWSAKLRGTSQVAVAFFGDGATNIGAFHE
                     ALNLAAVWKLPVLFVCENNLYMEYTSIKTVTAVAQPAAGRAPAYQLPAEVIDGNDVVA
                     VQRAVAAAAARARAGDGPTVIEALTYRHHGHSRTDPGKYRPQEEVDAWLKRDPLLVLA
                     ERLRDRGVDQPAIDAADTRAREQVAAAVEAAKAAPPPDEATAFTDVWADGGAQWRT"
     gene            complement(2591..>2800)
                     /locus_tag="VFO47_11990"
     CDS             complement(2591..>2800)
                     /locus_tag="VFO47_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET8789457.1"
                     /translation="ALETYWPAADGWSAARIEEEVVVTADGCEVITKFPAEELLVAGQ
                     RYWAVDGPLPATREAESHRNRRVTP"
BASE COUNT          346 a         1057 c         1029 g          368 t
ORIGIN      
        1 cagcactgcc accgggccga cgacatcgcc accgccctgc gcctggccga cgagttcggc
       61 taccggctgg tcatcgacca cggcaccgag gccgtcctgc tcgccgaccg cctggccgag
      121 cgccaggtgc ccgtcctgat cggccccctg ctgacctccc gctccaaggt cgagctgcgc
      181 aaccgctccc tggccaaccc gggccgcctg gccgccgccg gggtcgagct cggcatcatc
      241 accgaccacc cggtcgtgcc cgtccacctc ctccacgtcc aggccgccct ggccgtccgt
      301 gagggcctcg acccggccga cgccctccgc gccgtcaccc tcaccccggc ccgcgtcctc
      361 ggcctcgagg accgcctcgg ctcgctggag ccgggcaagg acgccaccct gtgcgtctgg
      421 tccggcgacc ccctcgacgc ccgctcccgg gtcgagtccg cctggatcga aggccgccag
      481 gtctacggcg cggaccccga cgacccgccg ttctagcctc cgcgggagcg ccacggttgc
      541 cgtcaccggc cggacgccgg acaccgaccc ggcgacccgc ttcggcggcc agaaccccga
      601 gctcgccgag ccggccgtga gcggctaggc gtcaatgacc tcccggacgg tggtcgcgac
      661 ccggtcggcg gtggggacga cggcctgctc caggttggcg gcgaagggca ggggaacgca
      721 ggcgctggcg acccggcgga tgggggcgtc gaggtcgtag aaggcctcct cggccacgat
      781 cgaggcgacg gtgccgcccc aaccgccctg gtaggggttc tcctcgacga tgaccaggcg
      841 gctggtgcgg gcgaccgagc cgagcaccgt gcgggcgtcc agggggatca ggcagcgcag
      901 gtcgatcacc tcggtgtcga ccccgtcggc ggccagctgc tcggccgcgg ccatggccgt
      961 gggcacggtc gaggccaggg cgaccagggt gacgtcgccg ccgggacggc ggacctcggc
     1021 ggcgcccagg ggcacgacgt ggtcgccgga gggcggcggg tcgcccttgg aggcgaacag
     1081 cgacttgtgc tcgaacacca gcaccgggtc gtccgagcgg atggcggcgg ccatcaggcc
     1141 gaccatgtcg gccggggtcg agggggcggc gatcttgagg ccggggacgg cgaagggcca
     1201 gttctcgacc gcctgggagt gctgggcgcc gaagccgagg ccgccgccgt tggccgtgcg
     1261 gacgaccagg gggacggtga cctggccgcc ggtcatgtag cgcatcttgg ggatctcgtt
     1321 ggcgacgtag tcccagcagc aggccaggaa gtcggagaac atgatctcgg ccaccggccg
     1381 cagcccggtc atggccgcgc ccatggccgc gccgacgatc gcctgctcgc tgatcggggt
     1441 gtcccagacc cgctcggggc cgaactcggc gaacagcccc gcggccgtct tgaacacgcc
     1501 ctcggcggcg ccgatgtcct cgcccaggca gaccacggtc tcgtcgcgcc gcatctcctg
     1561 ggcgatcccg gccgcgaccg cctcccggta ggtgatcagg tccgccattg ggcacctccg
     1621 tccgcccaca cgtcggtgaa cgcggtcgcc tcgtcgggcg gcggggccgc cttggccgcc
     1681 tccacggcgg ccgcgacctg ctcgcgggcc cgggtgtcgg cggcgtcgat ggccggctgg
     1741 tcgaccccgc ggtcgcgcag ccgctcggcc aggaccagca gcgggtcgcg cttgagccag
     1801 gcgtcgacct cctcctgggg acggtacttg cccgggtcgg tgcgcgagtg cccgtggtgg
     1861 cggtaggtga gggcctcgat caccgtcggg ccgtccccgg cccgggcccg ggcggccgcc
     1921 gccgccaccg cccgctgcac ggccacgacg tcgttgccgt cgatcacctc ggccgggagc
     1981 tggtaggcgg gagcccggcc ggcggccggc tgggcgaccg cggtcaccgt cttgatcgag
     2041 gtgtactcca tgtagaggtt gttctcgcag acgaacagca ccggcagctt ccacaccgcg
     2101 gccaggttga gggcctcgtg gaaggcgccg atgttggtcg cgccgtcgcc gaagaaggcc
     2161 accgcgacct ggctggtgcc gcgcagcttg gccgaccagg cggccccgca ggcgatcggc
     2221 aggtgggccc cgacgatggc gtacgacccg agcatgttgt ggtcggccac ggtcaggtgc
     2281 atcgagccgc ccttggcgcc gcagaggccg gtggcccggc tcatcagctc ggccaggcag
     2341 gcctcggggc tggccccgcg ggcgatggcg tggtggtggc cgcggtaggt ggcgaacacg
     2401 tagtcgtccg gctccagggc ggcgctggcc cccacggcca ccgcctcctg gccgttggcc
     2461 aggtgggtcg tgcccttgac cagcccggcc atgaacaggt cgaaggcggc cttctcggtg
     2521 gcccggatca gcgccatccg ctcgtacagc tcgagcagct cctcgacctt cggctcctcg
     2581 gcgacgacgg tcatggggtc acccgccggt tgcggtggga ctcggcctcc cgggtggccg
     2641 gcagcggccc gtccacggcc cagtagcgct ggccggcgac cagcagctcc tcggccggga
     2701 acttggtgat cacctcgcag ccgtcggcgg tgaccaccac ctcctcctcg atccgggccg
     2761 ccgaccagcc gtcggccgcc ggccagtagg tctccagggc
//
LOCUS       DASSWR010000715         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1410843, whole genome shotgun sequence.
ACCESSION   DASSWR010000715 DASSWR010000000
VERSION     DASSWR010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410843"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFO47_11995"
     CDS             <1..216
                     /locus_tag="VFO47_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789458.1"
                     /translation="KLAASTGEPLQKVLERAVENYRREQFYAEFNAAFERLRADPVAW
                     EEELAERPELEERSLTGSMICEDHPPR"
     gene            241..447
                     /locus_tag="VFO47_12000"
                     /pseudo
     CDS             241..447
                     /locus_tag="VFO47_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(499..1308)
                     /locus_tag="VFO47_12005"
     CDS             complement(499..1308)
                     /locus_tag="VFO47_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET8789459.1"
                     /translation="MLVDRTGAWWRATRTPAGPATARYSAEADGFRVAAWGPGAEWCL
                     EAAPELLGSRDSLEGFSPTGLVGELHHHFPGLRISRSRAVFEALLPSILEQKVTGREA
                     HNAFSAIVRAWGEPAPGPVRLRLQPPPDLLAVTPYWAFHPLGVERKRTTAIQFAASRA
                     RRVEETVAMAAPDAHRRLQALPGVGPWTSAEVAIVALGDADAVSVGDFHLPHLVSYAL
                     TGERRGTDERMLELLAPWPGHRGRVLRLLTRSGRYPDRRGPRMAVRAFTRI"
     gene            complement(1568..1870)
                     /locus_tag="VFO47_12010"
     CDS             complement(1568..1870)
                     /locus_tag="VFO47_12010"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET8789460.1"
                     /translation="MTELLVIPSELERAAGRLQASADQVRSVVGSLLPAVGGVGAALG
                     DAALADRFQALWSRWASELGVLADDLTGAAGRLQAAAAEYRSTDRSAVPPPSLHAR"
     gene            2097..>2616
                     /locus_tag="VFO47_12015"
     CDS             2097..>2616
                     /locus_tag="VFO47_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8789461.1"
                     /translation="MPGRFGRVLTAMATPFSPDGNLDVDGAQRLASHLLDSGTDTLVV
                     AGTTGESPTLTADEKARLWAAVVEVGGGDRVMAGTSTYSTAESVELTRLAEKAGAGSL
                     LLVTPYYNKPPQSGLVAHFSAIAGATGLPCMLYNIPGRTACTIEVPTMVRLFERLDNV
                     VAVKDAVGDMAAA"
BASE COUNT          362 a          924 c         1007 g          323 t
ORIGIN      
        1 aagctggccg cgtcaaccgg agagccgttg cagaaggtgc tcgaacgggc cgtcgagaac
       61 taccgtcgcg agcagttcta tgccgagttc aatgccgcct ttgagcggct ccgtgcggac
      121 ccggtcgcgt gggaggagga gctcgccgag cggccagagc tggaggaacg ctcgctgacg
      181 ggctcgatga tctgtgagga ccatccgccg cggtgaggcc cagcggatct tgtcgtcgcg
      241 gttcccttca ccacgcgccg gcgggccatc ccaacccacg tcgaggttcg cccaccgcac
      301 ggagggttgc gcgacgtcag cttcgccatg tgcgagcagg tccgctcgct gtctgccgag
      361 cggctcggcg cccgtccgtt cgggagcgtg ccatcggccg tgctgcgttc ggtggaggat
      421 cggctgcggc tcctgctcga actctgaagc ctggccgccc gggcgggacg accgcatcga
      481 cgaggccccg cattcggctc agatccgggt gaaggcgcgg accgccatgc gggggccgcg
      541 gcggtcgggg tagcggccgg agcgggtgag gaggcggagg acgcggccgc ggtggcccgg
      601 ccagggggcg aggagctcga gcatgcgctc gtcggtgccg cgacgctccc cggtgagggc
      661 gtagctgacc aggtggggga gatggaagtc gccgaccgag acggcgtcgg cgtcgccgag
      721 ggcgacgatc gcgacctcgg ccgaggtcca ggggccgacg ccggggaggg cctggaggcg
      781 gcggtgggcg tcgggggcgg ccatggcgac ggtctcctcg acccggcggg cgcggctggc
      841 cgcgaactgg atggcggtgg tgcgcttgcg ctcgaccccg agcgggtgga aggcccagta
      901 gggggtgacg gccagcaggt cggggggtgg ctgcagacgc agccgcaccg ggccaggggc
      961 gggttcgccc caggcgcgga cgatggccga gaaggcgttg tgggcctcgc ggccggtcac
     1021 cttctgctcc aggatcgagg gcagcaatgc ctcgaacacc gcccgggagc gggagatgcg
     1081 caggccgggg aagtggtggt ggagctcccc gaccaggccg gtgggggaga acccctccag
     1141 cgagtcccgc gaccccagca gctccggcgc cgcctccagg caccattccg cccccggccc
     1201 ccaggccgcg acccggaacc cgtccgcctc ggcgctgtac cgggccgtgg ccggccccgc
     1261 cggcgtccgg gtcgcccgcc accaggcccc cgtccggtcg accagcatgg tcgggtcccc
     1321 cggcccccgc cgcaacggcc ccagcgtcag ccggagatcc accggcaggc gcggccggac
     1381 gacagtctcg atcggagaat ccgcggcggt catcgcccgg atcttggcac gcccagccga
     1441 caaccacgag ccctgggtcc accgctcagg ccggtttcgg ggcccaggca ccgggtggag
     1501 ccggcccggg caggggaggg gacccgggcc cggtctggcc ggccgggccc gggtgggtcg
     1561 ccgtgcccta ccgggcgtgg agggagggtg gaggcacggc ggaccggtcg gtggagcggt
     1621 actcggcggc ggcggcctgg aggcggccgg cggcgccggt gaggtcgtcg gcgaggacgc
     1681 cgagctcgct ggcccagcgg ctccacaggg cctggaaccg gtcggcgagg gcggcgtcgc
     1741 cgagggcggc cccgaccccg ccgacggccg ggagcaggct gccgaccacc gaacggacct
     1801 ggtcggcgct ggcctggagc cgtccggcgg ctcgttccag ctcgctcggg atcaccagca
     1861 gctcggtcat ccacttcacc ccttcgccag ccagtggaag ctggcataga cgataacaga
     1921 cagaggcaaa cgaaagcaaa tgaagggtaa tgagcggcta acggctcccg gcccatcagc
     1981 cacgggcggc gaacatcccc ggtccggggt ggatgcccga gtagaatctg gagggctgcc
     2041 ggcggggtgg ggctgccggc cgtcacgccg cgttcccgtc gttcggagga cccgccatgc
     2101 caggtcgctt cggccgtgtg ctcaccgcca tggccacccc gttcagccct gacggcaacc
     2161 tggacgtcga tggcgcccag cgcctggcca gccacctgct cgactccggt accgacaccc
     2221 tggtcgtggc cggcacgacc ggcgagtcgc ccaccctgac cgccgacgag aaggcccgcc
     2281 tgtgggcggc ggtcgtcgag gtcggcgggg gcgaccgggt catggccggc acctcgacct
     2341 actcgaccgc cgagtcggtc gagctcaccc gcctggccga gaaggccggg gcggggtcgc
     2401 tgctgctggt gacgccctac tacaacaagc cgccccagtc cggcctggtg gcccacttct
     2461 cggccatcgc cggcgccacc ggcctgccct gcatgctcta caacatcccc ggccggaccg
     2521 cctgcaccat cgaggtgccg accatggtcc ggctgttcga gcgcctcgac aacgtggtcg
     2581 cggtcaagga cgcggtcggg gacatggccg cggcca
//
LOCUS       DASSWR010000716         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1186077, whole genome shotgun sequence.
ACCESSION   DASSWR010000716 DASSWR010000000
VERSION     DASSWR010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186077"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1981
                     /locus_tag="VFO47_12020"
     CDS             95..1981
                     /locus_tag="VFO47_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789462.1"
                     /translation="MPASSIGSTTEEAVAAPPEVVAPVEEGRETRRQRLRLLARRLRP
                     GPEPVFLLVFCGLAMWLFAPAWSSPTTRTLEGGDGDPAIFMWFLRWVPFALEHGHDLL
                     VTHHLNYPDGVNLMWNTSLPLPGLLLAPVTGAWGPVLSFNLLLVLAYGLSAWCAYLAI
                     RRFAPGHLAAAAGGLVYGFSPAIRGQSHHLHMSLAFLVPLMLLALHEILVRQRRSPWL
                     VGAGLGLMAGAQLLIGEELLAMTALLGFAMFVLLLLANLRRLRARWAYAAKAFAVALV
                     MVSAIVVWPLSVQFGGPQRIHGDIQKTNYSNDLQGFVVPGWAQALTWDGAGELVAGFA
                     GGNSAYLGLPMLLVLAALGVRWRSSPVVRTGLALMAVAAVLSLGPTLLVGGEDTGIPL
                     PWAFFENLPLLPSLIPSRLAQFTALFAGLLLAVFLQAVWRGGGWRRPAAVVVAVVVLL
                     PLWPSATIPAEEVTTPAFFTGPGVRELPRDSVALVLPWPYRRTALAMTWQVEAGLWYR
                     MPGGYFIGPQRDSDQPRFDAIPSPASITFAQIFAGRPPPRLTGPRRRALARDLVRWRV
                     GSVIVGPMPNQAAMVAFLTDLLGREPQLVEGVYLWRDPVVELRDRGQDRREGDRGPAR
                     RPRP"
     gene            complement(1917..>2163)
                     /locus_tag="VFO47_12025"
     CDS             complement(1917..>2163)
                     /locus_tag="VFO47_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789463.1"
                     /translation="IGKRVNLERTDEALATGADVVGTACPYCMVMLDDAIRQRQAEGD
                     GEGVRVLDVAQVLEQSVTAGDAVRAPGRPHAGPDPGP"
BASE COUNT          218 a          804 c          789 g          352 t
ORIGIN      
        1 acagcgggga ccgaccccgt cccggtactg ggagaacttc cgctcgtcct gaccccaacg
       61 ccaggcccgg atcgaggact gccatttgtc gaacttgcca gcatcatcca tcggctcgac
      121 caccgaggag gccgtcgcgg ccccgcccga ggtcgtggcc ccggtggagg agggccgcga
      181 gacccgccgg cagcggctgc gcctgctggc ccggcggctg cggcccggcc ccgagccggt
      241 gttcctgctg gtgttctgtg gcctggccat gtggctgttc gccccggcgt ggtcgtcgcc
      301 gaccaccagg accctggagg gcggggacgg cgacccggcg atcttcatgt ggttcctgcg
      361 ctgggtgccg ttcgccctcg agcacgggca cgacctgctg gtcacccacc acctcaacta
      421 ccccgacggc gtgaacctga tgtggaacac gtcgctgccc ctgcccgggc tgctgctggc
      481 cccggtgacc ggagcctggg ggccggtgct cagcttcaac ctcctgctgg tgctggccta
      541 cggcctctcg gcctggtgcg cgtacctggc catccggcgc ttcgcccccg ggcacctggc
      601 ggcggcggcc ggcgggctgg tctacggctt ctccccggcg atccggggcc agtcgcacca
      661 cctgcacatg tcgctggcct tcctggtccc gctgatgctg ctggccctgc acgagatcct
      721 ggtccgccag cggcgctccc cctggctggt cggggccggc ctcggcctga tggccggcgc
      781 ccagctcctg atcggcgagg agctgctggc catgacggcc ctgctcggct tcgccatgtt
      841 cgtgctgctg ttgctggcca acctgcggcg gctgcgggcg cggtgggcgt acgccgccaa
      901 ggcgttcgcg gtcgccctgg tgatggtgtc ggcgatcgtc gtctggccgc tgtcggtgca
      961 gttcggcggg ccgcagcgga tccacgggga catccagaag accaactact ccaacgacct
     1021 ccagggcttc gtcgtgcccg gctgggccca ggcgctgacc tgggacgggg ccggcgagct
     1081 ggtggccggc ttcgcgggcg gcaactccgc ctacctgggc ctgccgatgc tgctggtgct
     1141 ggccgcgctc ggggtccgct ggcgctccag cccggtggtc aggacggggc tggccctgat
     1201 ggcggtggcg gcggtgcttt cgctggggcc gaccctgctg gtgggtgggg aggacaccgg
     1261 gatcccgctg ccctgggcct tcttcgagaa cctgccgctg ctccccagcc tcatcccgtc
     1321 ccggctggcc cagttcaccg ccctgttcgc cggactcctg ctggccgtgt tcctccaggc
     1381 cgtgtggcgc gggggcggct ggcggcggcc ggccgcggtg gtggtggccg tggtcgtgct
     1441 gctgccgctg tggccgtcgg cgacgatccc ggccgaggag gtgaccaccc cggcgttctt
     1501 caccggcccg ggcgtgcgcg agctgccccg tgacagcgtg gccctggtgc tgccctggcc
     1561 gtaccggcgg accgccctgg ccatgacctg gcaggtcgag gccggcctct ggtaccggat
     1621 gcccggcggc tacttcatcg gcccccagcg cgacagcgac cagccgcgct tcgacgccat
     1681 cccgagcccg gcctcgatca ccttcgccca gatcttcgcc ggccgcccgc cgccgcgcct
     1741 caccgggccg aggcgtcggg ccctggcccg cgacctggtc cgctggcggg tcgggagcgt
     1801 gatcgtgggc ccgatgccca accaggcggc gatggtggcc ttcctgaccg acctgctggg
     1861 gcgcgagccg cagctggtcg agggcgtcta cctgtggcgc gacccggtgg tcgagctcag
     1921 ggaccggggt caggaccggc gtgagggcga ccgggggccc gcacggcgtc cccggccgtg
     1981 accgactgct cgagcacctg ggcgacgtcc aggacccgga cgccctcgcc gtcgccctcg
     2041 gcctggcgct gcctgattgc gtcgtccagc atgaccatgc agtaggggca ggctgtcccg
     2101 accacgtcgg cgccggtggc cagggcctcg tcggttcgct cgaggttgac ccgcttgccg
     2161 atc
//
LOCUS       DASSWR010000717         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_281653,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000717 DASSWR010000000
VERSION     DASSWR010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281653"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFO47_12030"
     CDS             complement(<1..187)
                     /locus_tag="VFO47_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8789464.1"
                     /translation="MTGTGPVRVAIACQGGGSHTAFTAGVLKRLLGAKELAGVEVVGL
                     SGTSGGAVCALLAWSALL"
     gene            complement(289..1221)
                     /locus_tag="VFO47_12035"
     CDS             complement(289..1221)
                     /locus_tag="VFO47_12035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET8789465.1"
                     /translation="MAAIEPPETSRVARLLRRRPPAAALRWAAAPFGPGSRVVAVRPL
                     PSAWLANHAVDVADAAGATHRLVLRRWARPGWDDEDPDFTAAREAAILELLAPTPVPA
                     PPLVAADADAAVCDVPALLLSRLPGGPPDLHDPAALVEGLAAALPPIHAVTVPATRVV
                     PPHHRFYEPATLVPPAWSDRPELWERAFTVAAGPPPDDRACFIHRDYHPGNTLWTGGR
                     LTGVVDWIGGSWGPPSVDLGHMRVNLTADLGLAVADRLLAAHRALTGFDHHPWWDVAA
                     TVDVVPETPPAWRGVEDLVAAALARLGGEVGKAG"
     gene            1263..1775
                     /locus_tag="VFO47_12040"
     CDS             1263..1775
                     /locus_tag="VFO47_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HET8789466.1"
                     /translation="MDRAPSLLPPLPPPVRALLLGFVTGIRSQVPVALLAIEARQGRF
                     DPGAGRLARRFASPQGTGGALVAFAGELVADKLPMTPRRTTWGPFSQRLVTGGTIGAA
                     VQYDAGESRPLGALLGAAGAGAGAYAATKVRALAAERTRLPGPLLGAAEDLFAVALAV
                     AAVTAGRDSG"
     gene            1814..2527
                     /locus_tag="VFO47_12045"
     CDS             1814..2527
                     /locus_tag="VFO47_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8789467.1"
                     /translation="MEVADGIHRFGTRMVNWYLIERGGRITLVDAGMRGYWPQLTDAL
                     TALGGKVEAVEAVVLTHAHADHVGFAHQVKANSDATVWVHERDAEPGLRRFPPFRLYL
                     RPTAWPLLVHGLRNGLLATPDAAEVRTFTDGDVLDVPGRPRVRHLPGHTRGNCALHLP
                     DASVVFSGDTLVTFDPYRRRRGPRLLVKGVNEDNEQARSSLDALAGLNAQLLLPGHGE
                     PWRDGVAAAVADARRRGYW"
     gene            complement(2535..>3534)
                     /locus_tag="VFO47_12050"
     CDS             complement(2535..>3534)
                     /locus_tag="VFO47_12050"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET8789468.1"
                     /translation="LPYVAPEQARDERAGPAADLWALGATLWFAVEGVAPFERAGPAA
                     PLAAVLHDPPGRPARAGPLEPVLLALLTKDPGARPGAAAVRGMLAPTATGSPGPTAPT
                     TAGPRGPAAPTAGTTRLPGDPAAATEVLVGAAPPPGWGEPRPAPLGRRPRGRVQLPGW
                     TAPPRRKRRWGRRLLVAAGVLALLAVASNLGDAERRADSAPAGLGDPVRDGQFEFVVR
                     SVDCGAASVGRGLAPKTAQDQFCLVALEVENTGTEGRTFGGGQQFLFDSDGNRHDPDL
                     DATARHEGDARLWSSHLNPGQRLEGTLVYDVPESATPSRVELHDGPLSGGASVAVA"
BASE COUNT          399 a         1385 c         1365 g          385 t
ORIGIN      
        1 cgagcagcgc cgaccaggcg agcagggcgc agacggcccc gccggaggtg ccgctgagac
       61 cgacgacctc gaccccggcc agctccttgg cgccgaggag gcgcttgagc accccggccg
      121 tgaaggcggt gtggctgccg ccgccctggc aggcgatcgc tacccggaca ggtcccgttc
      181 cggtcatccg tcccatgcta cgggaccgct tgtttgctcc gctgctgcgc gagttacggg
      241 cggggcagtc ccgcagcctg agctgcggga cgacgacggg cagaacgcct agccggcttt
      301 gccgacctcg ccgccgaggc gggccagggc ggcggcgacc aggtcctcca cgccgcgcca
      361 ggccggcggc gtctcgggga cgacgtcgac cgtcgcggcc acgtcccacc aggggtggtg
      421 gtcgaagccg gtcagggcgc ggtgggcggc caggagccgg tcggcgacgg ccaggccgag
      481 gtcggcggtc aggttgaccc gcatgtggcc caggtcgacc gagggcggcc cccacgaccc
      541 gccgatccag tccaccaccc cggtgagccg gccgccggtc cagagggtgt tccccgggtg
      601 gtagtcgcgg tggatgaagc aggcccggtc gtccggcggc ggcccggccg ccaccgtgaa
      661 cgcccgctcc cacaactccg gccggtccga ccaggccggc gggaccagcg tcgctggttc
      721 gtagaagcgg tgatggggag gcacgacgcg ggtcgccgga acggtcacgg catggatggg
      781 cgggagggcc gcggccagac cctcgaccag ggcggccggg tcgtggaggt cgggcgggcc
      841 gccgggaagg cggctgagca gcaacgcggg cacgtcgcag acggcggcgt cggcgtcggc
      901 ggcgaccagc ggcggcgccg gcaccggggt gggggcgagg agctccagga tggccgcctc
      961 gcgggcggcg gtgaagtcgg ggtcctcgtc gtcccagccg ggtcgcgccc agcggcgcag
     1021 caccagccga tgggtcgcgc cggccgcgtc ggccacgtcg acggcgtggt tggccagcca
     1081 ggccgacggc agcggccgca ccgccaccac ccggctcccg gggccgaacg gcgccgccgc
     1141 ccagcgcagg gcggccgccg gcgggcggcg gcggagcagc cgggcgaccc ggctggtttc
     1201 gggaggctcg atcgcggcca tgcggctagc atcatgcacc ccgccgaacc gaaggagccg
     1261 ccgtggaccg ggccccctcc ctcctgccac ccctgccccc gcccgtcagg gcgctgctgc
     1321 tcgggttcgt caccggcatc cgttcccagg tcccggtcgc cctcctggcc atcgaggcca
     1381 ggcagggccg cttcgacccg ggcgcgggcc ggctggcccg ccggttcgcc tcgccccagg
     1441 ggacgggcgg cgcgctggtc gccttcgccg gcgagctggt cgccgacaag ctccccatga
     1501 ccccgcgccg gaccacctgg ggcccgttca gccagcgcct ggtcaccggc gggacgatcg
     1561 gcgccgcggt ccagtacgac gccggcgagt cgcgccccct cggtgccctc ctcggcgctg
     1621 ccggggccgg cgccggcgcg tacgccgcca ccaaggtgcg cgccctggcc gccgagcgca
     1681 cccgcctccc cggccccctg ctcggcgcgg ccgaggacct gttcgccgtc gccctggccg
     1741 tcgccgcggt caccgccggc cgcgactccg gctagaaggg tttgcggcgc cgatctcgtg
     1801 caaggccgtc gccatggagg tagccgacgg catccaccgg ttcggcaccc gcatggtcaa
     1861 ctggtacctg atcgagcgcg ggggccgcat caccctggtc gacgccggca tgcgcggcta
     1921 ctggccgcag ctcaccgacg cgttgaccgc cctcggcggc aaggtcgagg cggtcgaggc
     1981 ggtcgtcctc acccacgccc acgccgacca cgtcggcttc gcccaccagg tgaaggccaa
     2041 ctccgacgcc accgtctggg tccacgagcg cgacgccgag cccgggctgc gccggttccc
     2101 gccgttccgg ctgtacctgc ggccgaccgc ctggccgttg ctcgtccacg gcctccgcaa
     2161 cgggctgctc gccacccccg acgccgccga ggtccgcacc ttcaccgacg gcgacgtcct
     2221 cgacgtcccc gggcggcccc gggtccgcca ccttcccggc cacacccgcg gcaactgcgc
     2281 cctgcacctg ccggacgcca gcgtcgtctt cagcggcgac accctggtca ccttcgaccc
     2341 gtaccggcgg cgtcgcgggc cgcggctgct cgtcaagggc gtcaacgagg acaacgagca
     2401 ggcccggagc tccctggacg cgctggccgg gctgaacgcg cagctgctgc tccccggcca
     2461 cggcgagccc tggcgcgacg gggtggccgc ggccgtggcc gacgcccgcc gccgcggtta
     2521 ctggtgaggg gcgctcaggc caccgcgacg ctggcgccgc cgctgagggg gccgtcgtgg
     2581 agctcgaccc gtgacggggt ggcggactcg gggacgtcgt agacgagcgt gccctccagg
     2641 cgctggcccg ggttgaggtg gctggaccac agccgggcat cgccttcgtg gcgggcggtc
     2701 gcgtccaggt ccgggtcgtg gcggttgccg tcgctgtcga acaggaactg ctggccgccg
     2761 ccgaaggtgc ggccctcggt gccggtgttc tcgacctcga gcgcgaccag gcagaactgg
     2821 tcctgcgcgg tcttgggcgc gaggccccgg cccacggacg cggcaccgca gtcgaccgag
     2881 cggaccacga actcgaactg accgtcccgg accgggtcgc ccagcccggc cggggcactg
     2941 tcggcccgcc gctcggcgtc gccgaggttg ctggccaccg cgagcagggc caggaccccg
     3001 gcggcgacca gcagccgccg cccccagcgc cgcttccgcc gcggcggggc ggtccagccc
     3061 gggagctgca cccggccccg cggccgtcgc cccaagggcg ccgggcgcgg ttcgccccag
     3121 ccgggcgggg gagcggcccc gaccagcacc tcggtggcgg ccgccgggtc gccggggagg
     3181 cgcgtcgtgc cggccgtcgg cgcggccggg ccgcggggac cggcggtcgt cggggcggtc
     3241 gggccggggg acccggtggc cgtcggcgcc agcatgccgc ggacggccgc ggccccgggg
     3301 cgggcgcctg ggtccttggt cagcagggcc agcaggaccg gttccagcgg cccggcccgg
     3361 gccgggcggc cgggcgggtc gtggaggacg gcggcgaggg gggcggccgg gccggcccgc
     3421 tcgaacgggg ccaccccctc gaccgcgaac cacagcgtcg cccccagcgc ccacaggtcg
     3481 gccgccggcc cggcccgctc gtccctggcc tgctccgggg ccacgtacgg cagc
//
LOCUS       DASSWR010000718         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_84504,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000718 DASSWR010000000
VERSION     DASSWR010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84504"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..928
                     /locus_tag="VFO47_12055"
     CDS             344..928
                     /locus_tag="VFO47_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789469.1"
                     /translation="MKRRSHRPTLALLAIATAVALSGCQQGSDPTSANSGTGERPPAS
                     PPAAPTSRAAPAQQAGRASSATTAARVRDFGFIRSWYAKTGTIYLRFDRAVLLTGEAA
                     DDASAAHGGESPVPNDLYIQNDNPRLRDLAIVDQAKVIGSQQLTGSPGPNPSNLKALL
                     TFVHKGGSRVAATPFHLTYNDNGLVVRVQEQYLP"
     gene            1014..1913
                     /locus_tag="VFO47_12060"
     CDS             1014..1913
                     /locus_tag="VFO47_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006901250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8789470.1"
                     /translation="MSVIRIEGLTKRFGAVLAVDNLNFVVAQGTVTGFLGPNGAGKTT
                     TLRTLLGLITPTAGTTRIHGRPYRQLTDPIRHVGALLESSGFHPGRSARNHLQVVATA
                     AGLPAARADEVLAQVGLAGAARRRVSGFSLGMRQRLGLATALLGDPQVLVLDEPANGL
                     DPEGVHWLRGLLRHLADQGRTVLVSSHVLAEIAQTVDQVVIIAAGRLVTQSTLAALTD
                     QAKGAVRVRSADPQALRDLLVRQGVSVELTASDELVASGVSSEQIGRVAAGANIAIYE
                     MRSESANLEKAFLQLTGPQGGTS"
     gene            1910..2653
                     /locus_tag="VFO47_12065"
     CDS             1910..2653
                     /locus_tag="VFO47_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8789471.1"
                     /translation="MTHLIHAELAKLRTTRTTPMLLAAMVGFAIITVIFVVTVSGTQG
                     NPPLDADSLRHLVAAPSRVISGTALLVGILALTGEFRHQTITQTFLITPNRGRVVAAK
                     LAAGILVGLVLGVTSTVAVLTVGLPWLAAKGVPIQLDGGFGQTVAGVLAAAALSGLLG
                     VAVAALVRNQVAAVVGVLLWFLVIEGILPAVLGAPTLPRWLPGGAAAALTDPGGRYLS
                     LWVGGLLLAGYGLVLAAIGSRLTLRRDIT"
     gene            2716..4029
                     /locus_tag="VFO47_12070"
     CDS             2716..4029
                     /locus_tag="VFO47_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8789472.1"
                     /translation="MTRISSWKVAVAALLLAGCAGTGTASTTAGTDSKPPDHQIHYCK
                     NVDAYRIQDCAKAILPATPLGQQLQWVLDQLGGDAATLTETEVREHVSAEFLTLVMPP
                     EAVIGALQGSLAEQGAQRFVGFAYPPREREALAIVQNATGHRGAIPIGVSAGRPALIE
                     ALGGENAPPTIVPRGRFSGWFDVGGRRLFLRCSGHGSPTVVFEGGVTHHWFDLQNQLA
                     RFTRVCTWDHPNGPISRSDPAPTPRTARDFVADLHALLRTARVPGPYVLAGGSNGGLY
                     TQLYASTHPHQVAGLVLIDAVHSAYHKRRLAMFKPLLPPGQWEALRQDAMAVPHRLID
                     PERVDIWTSERQVRLALRRAPLRPMPLVALARGRPANPGAPFVEQDERLWRQLQRELA
                     RLVPGGRLVVATQSGHDVQDEQPELVLDAVRDVVQAVRSGDLVPH"
BASE COUNT          654 a         1725 c         1375 g          612 t
ORIGIN      
        1 ccggctgtcg gaggcggcga gatcccacag cccgaacagc acccccccca ctctggccct
       61 ggactgtcgg tgccggggca ccgccgtcag ccagcatccg ctcggccgcc ccgcgctcac
      121 ccgctgagtg aagcctgcgg aacacgatcc ccggcatggg cggtaagggt agatgcatcg
      181 tctgctccgg tcaggggcga agggctttcc cgcatccttg cgcccggcag ttggcgccgg
      241 cttggcggcc ttgcccccca gcccaccaac tcgctgccaa gtcgccacca agccgccctc
      301 ccatgctggg ctcacgacag ccccgcacgg gaggagagat gcagtgaaac ggcgaagcca
      361 ccgcccaaca ttggcattac ttgccatcgc gacagcggtc gcgctcagtg ggtgccagca
      421 aggatctgac ccgacgagcg ccaacagcgg caccggcgag cggccgccgg ccagcccgcc
      481 ggccgcgcct acgtcgaggg ccgccccggc ccagcaggcc ggtcgggcat cgagcgccac
      541 cacggcggcc cgggtccgcg acttcgggtt catccgcagc tggtatgcca agaccggcac
      601 gatctacttg cggttcgacc gggcggtcct gctgaccggc gaggccgccg acgacgcctc
      661 ggctgcccac ggcggcgagt cgccggtccc caacgacctc tacatccaga acgacaaccc
      721 gcgcctgcgc gacctggcca tcgtcgatca ggccaaggtg atcggcagcc agcagctcac
      781 gggcagccca ggacccaacc cgagcaacct gaaggccctg ctcaccttcg tccacaaggg
      841 cgggtcgcgg gtcgcggcca ccccgtttca cctcacctac aacgacaacg gcctggtcgt
      901 ccgggtccag gagcaatacc tgccctgacc accgggacct cggtcccatc tcgaggactg
      961 cgccaccacc cccggcctcc catgccatcc cgccacccac aaggaggact gccatgtcgg
     1021 tgatcaggat cgaaggactg accaagcgct tcggtgcggt gctcgctgtc gacaacctca
     1081 acttcgtggt cgcccaaggc acggtgacgg gcttcctggg ccccaacggg gccggcaaga
     1141 ccaccaccct gcgcaccctg ctcggcctga tcacccccac cgccggcacc acccgcatcc
     1201 acggccggcc ctaccgacag ctgaccgacc ccatccgcca tgtcggtgcc ctgctggaat
     1261 cctccggctt tcatcctggc cgcagcgccc gcaaccacct gcaggtggtc gccacggccg
     1321 ccggcctgcc agcggcccgg gctgacgagg tcctcgcgca ggtcggcctg gccggtgcgg
     1381 cccggcggcg ggtgagtggc ttctcgctcg gcatgcgcca gcgcctcggg ctggccactg
     1441 ccctgctcgg cgacccccag gtcctggtcc tggacgaacc ggccaacggc ctggaccccg
     1501 aaggcgtcca ctggctgcgc ggcctcctgc gccacctggc cgaccagggc cgcaccgtgc
     1561 tggtctccag ccacgtccta gccgagatcg cccagaccgt cgaccaggtg gtcatcatcg
     1621 ccgccggccg gctggtcacc cagtccaccc tggccgccct gaccgaccag gccaagggcg
     1681 cagtccgggt ccgcagcgcc gacccccagg cgctccgcga cctgctggtg cgccaaggcg
     1741 tcagcgtcga gctgaccgca tccgacgagc tggtggccag cggcgtcagc agcgagcaga
     1801 tcggccgggt agccgccggc gccaacatcg ccatctacga gatgcgctcg gagtccgcca
     1861 acctcgaaaa ggccttcctc cagctcaccg gcccccaagg aggcacgtca tgacccacct
     1921 gatccacgcc gagctggcca agctgcgcac cacccgcacc acccccatgc tgctggccgc
     1981 catggtcggc ttcgcgatca tcaccgtgat cttcgtcgtc accgtctcgg ggacccaggg
     2041 caacccgccc ctggacgctg acagcctgcg ccacctcgtc gccgccccct ccagggtgat
     2101 ctccgggacg gccctgctgg tgggcatcct ggccctgacc ggcgagttcc gccaccaaac
     2161 catcacccag accttcctaa tcacccccaa ccgcggccga gtcgtggccg ccaagctggc
     2221 cgccggcatc ctggtcggcc tggtccttgg tgtcaccagc acggtagccg tgctaacggt
     2281 tgggctgccc tggctggccg ccaagggcgt gcctatccag ctcgatggcg ggttcggcca
     2341 gacggtcgcc ggcgtgctgg ccgccgcggc cctgtccggg ctcctcggcg tcgccgtggc
     2401 cgccctggtc cgcaaccagg tcgccgccgt cgtcggcgtg ctgctgtggt tcctggtcat
     2461 cgaagggatc ctgcccgccg tgctgggcgc ccccaccctg cctaggtggc ttcccggcgg
     2521 cgccgcggcg gcgctcaccg accccggcgg ccgctacctg tcgctgtggg ttggcggcct
     2581 gctgctggcc ggctacggcc tggtcctggc cgccatcggc agccgcctca ccctccgccg
     2641 cgacatcacc taggcacagt ccggatccct gcccgatccg gccggcctca tggaacgaga
     2701 aggagctgca tcgctatgac aagaatcagc agctggaagg tggcagtagc cgcactcctg
     2761 ctggcaggct gcgccggcac tggcaccgca tcgaccaccg cgggcaccga ttccaagccc
     2821 cccgaccacc agatccacta ctgcaagaac gtcgacgcct accggatcca ggactgcgcc
     2881 aaggccatcc tgcccgccac cccgctgggc cagcagctgc aatgggtcct ggaccagctc
     2941 ggcggcgacg ccgcgaccct gaccgagacc gaggtccgcg agcacgtctc ggccgagttc
     3001 ctcaccctgg tcatgccacc ggaggcggtc atcggcgccc tccagggatc cctcgccgag
     3061 cagggagccc agcgcttcgt cggcttcgcc tacccgccca gagaacgcga ggcgctggcc
     3121 atcgtccaga acgccaccgg tcaccggggc gcgatcccga tcggcgtcag cgccggccgg
     3181 ccggccctca tcgaggccct gggcggcgag aacgccccac ccaccatcgt gccacgcggc
     3241 cggttctcgg gctggttcga cgtcggcggg cggcggctgt ttctgcgctg tagcggccat
     3301 ggcagcccca ccgtggtgtt cgaaggcggc gtgacccacc actggttcga cctgcagaac
     3361 cagctggccc gcttcacccg ggtatgcacc tgggatcatc caaatgggcc cataagccgc
     3421 agcgaccccg cccccacccc acggaccgcc cgcgacttcg tggccgacct gcacgccctg
     3481 ctccggaccg cccgggtgcc cggcccttat gtgctggccg gcggctccaa cggcggcctg
     3541 tatacccagc tgtacgccag cacccaccca caccaggtgg ccgggctggt gctgatcgat
     3601 gccgtgcatt ccgcctacca caagcggcgc ctggccatgt tcaagccctt gttgccaccc
     3661 ggccagtggg aggcgctgcg ccaggacgcg atggcggtgc cgcaccggct catcgatccc
     3721 gagcgggtcg acatctggac cagcgaacgg caggttcgcc tggccctgcg gcgcgccccg
     3781 ctgcgaccga tgccgctggt cgcgctggcc cgtggccgcc ccgccaaccc tggcgcgccg
     3841 ttcgtcgagc aggacgagcg gctgtggcgc cagctgcaac gcgagctagc ccggctggtc
     3901 cccggcggca ggttggtcgt cgccacccag agcggccacg acgtccaaga cgagcaaccc
     3961 gagctggtcc tggacgccgt ccgcgacgtc gtccaggccg tgcggtcggg cgacctggtg
     4021 ccccactgac gaggttcgac tccacaaggt gatcgggcag gataccccca gagcgccgac
     4081 gtccgccgtc gccggacgta gctgggtgtc ctgcccatca tccctcaccc gctccgcaac
     4141 cgaccaggta gccagcgcca acccgacccc gtcttggcac tttccgccgg gtccagtcgc
     4201 gtgtcgaaac cggtcgttcg aggggaggag ctggatggtg gtcggcatcg ggtgcacatc
     4261 gccgcctgcg ttatggctgc cgcaacaccc ggagagatcc tggtgtccag gacgtgcacg
     4321 atctggtgac cggctctgca tcgcctgcag gatcggggaa cccatc
//
LOCUS       DASSWR010000719         5539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1186247, whole genome shotgun sequence.
ACCESSION   DASSWR010000719 DASSWR010000000
VERSION     DASSWR010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186247"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFO47_12075"
     CDS             complement(<1..289)
                     /locus_tag="VFO47_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8789473.1"
                     /translation="MTRAPAELRQVADLAAKTLAAAGAPERGDGVAVAVSGGADSLAL
                     LAAAVFEARRLALRVIGVTVDHGLQPGSRTHADHVVAQMAALGADETVAVRV"
     gene            complement(306..1433)
                     /locus_tag="VFO47_12080"
     CDS             complement(306..1433)
                     /locus_tag="VFO47_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET8789474.1"
                     /translation="MVSTALPTDVPELVDWQLAARVGRRVAGGSELPVRAAWSRQFTD
                     LSTTADRAVAEFTGLGGELPPPIAEPLDRGEWVEANLATLRRILRPLAEKVADRKAWK
                     ATGPVPAAMRTSTRAIAGAQAGTLLGYVGQRVLGQYDLPMPGPDQAVSPNGDAPLPVK
                     AHGEGTVWYVVPNIVATERRHRFRPADFRLWIALHETAHRRQFRGVPWLPGHIQGLLD
                     EYLGSVEVDDEALKRLSQRLQGLARRVIAGEKIELLDLLVTPEQKTIVDRVQSTMTVL
                     EGHGEFVMDQLGDRLVPGHKHMHDTLRARRNAPGAAERLIQQLLGFRQKLDQYAMGER
                     FVRRLFERGGMAAVNQVFAEPAALPTMDEVRDPDRYLARAG"
     gene            complement(1508..2134)
                     /locus_tag="VFO47_12085"
     CDS             complement(1508..2134)
                     /locus_tag="VFO47_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET8789475.1"
                     /translation="MHLAVARSLPRLLPLAIAVALLGAGLVLTAGGPAEAQSVPNQVT
                     IEGTAENTWVPADAAVRPGGTVTFEITGGTTHPVFSGDGSDIEKSDGKFDASDCTIAN
                     MTKVGDSCQVKFPKAGTFPYFCQVHVTQGMKGTITVGTTAAAATTTTQGEAGGAVVET
                     PSAAAPPSSGRPAIYWAGWGLFALGALLALVLIALYVRFWPGFSRPKS"
     gene            2328..2627
                     /locus_tag="VFO47_12090"
     CDS             2328..2627
                     /locus_tag="VFO47_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789476.1"
                     /translation="MNRAGGERGATAVAYAVMVAIIALLLVAGVFVLGGSIEQAFIGG
                     ADCIVSPGSCDDGSGGGGGGGGGGGGGGGGGGGGGGPTTTNPPGPTTTTTTTGGP"
     gene            complement(2633..2953)
                     /locus_tag="VFO47_12095"
     CDS             complement(2633..2953)
                     /locus_tag="VFO47_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET8789477.1"
                     /translation="MAPGDLGDHGRFSDPSLLVMSSLADGPKHGYAMTKDIQRFAGVT
                     LGPGTLYGALARLQHQGLIEALPADDRRRPYRLSEKGTKVLAAQLESLQQVATTGLRR
                     LARE"
     gene            3135..3335
                     /locus_tag="VFO47_12100"
     CDS             3135..3335
                     /locus_tag="VFO47_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789478.1"
                     /translation="MKIRKRLAQLGLIMVALPVVGWGLEQLAKRAEERRPGSGKWLRT
                     GSDFAHGFGRGPLSSRLRTPRR"
     gene            3361..4104
                     /locus_tag="VFO47_12105"
     CDS             3361..4104
                     /locus_tag="VFO47_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET8789479.1"
                     /translation="MRTAPRIGSELVGWRRLAVVAALANAVVLFGYGLTRGDREALVL
                     AVVMVVGLGLLRVGTGLLGLLVLAALFVNIEIWMFPAANGNATYRAGLLDLLVPASLV
                     AFSLAGFIGAVAALVHLRDPAAGRGAAGPTGVIAVLFIFISLGLVWAMTDAEPQVVPE
                     GAVEVQTTNVAFVPDTLRAGGGQVTVAVRNRDLFWHTFTIDALGVDIRAPVGGVRSAS
                     FEAEPGTYDFYCRIPGHAALGMRGKLTVG"
     gene            complement(4124..>5539)
                     /locus_tag="VFO47_12110"
     CDS             complement(4124..>5539)
                     /locus_tag="VFO47_12110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8789480.1"
                     /translation="QMAAGAGAAGARVAGTGAGAAVQVGAATKPVIVRGNTWFVRDTL
                     AGGVADHTFTHGQAGDFPLLGDWDGDGEATPGVVRGSSWLLRNSLSAGPADVSFSYGF
                     PGDYPVVGDWDGNGTDTPGVVRGSTWFLKNTNASPGVADVSFAYGFASDLAVAGDWNG
                     DGTSTPGVVRGATWFLRATNDPANTTTVPPFDFGLPSDQRVVGDWDGNGTTTVGVKRR
                     SAWTLRNSNQAGPADLTFSYGLGCDLGFAGPGAITRGRGGKGLPAALRGTELNTVATT
                     QNVVALTFDAGANASAVPSILSTLEANCVPATFFLTGRWVDNFPADTRRIAVRYPVGN
                     HSDTHPHLPALSDAAVRDEINRAERKIQGATKFNARPMFRFPFGDRDARTLAIVNGLR
                     YGSIRWTVDSLGWQGTSGGQSVQSVTNRVLASLRPGEIVLMHVGSHPTDGSTLDADAL
                     PGIIAELKRRGYGFVTIYDSI"
BASE COUNT          671 a         1935 c         2174 g          759 t
ORIGIN      
        1 gcacccgcac cgcaacggtc tcgtccgccc ccagtgcggc catctgcgcg acgacgtggt
       61 ccgcgtgcgt acgcgaaccc ggttgcagcc cgtggtccac ggtcactccg atcacccgga
      121 gcgccagtcg gcgggcctcg aatacggcgg cggcgagcag ggccagggag tcggcgcccc
      181 cggacaccgc caccgcgacc ccgtcgccgc gctccggcgc cccggccgcg gccagggtct
      241 tggcggccag gtcggcgacc tggcggagct cggccggcgc cctggtcatg gccgcgggcc
      301 ggccgctacc cggcccgggc gaggtagcgg tccgggtcgc gcacctcgtc catggtcggc
      361 agggccgccg gctcggcgaa gacctggttg accgcggcca tgccgccccg ctcgaacagc
      421 cggcgcacga accgctcccc catcgcgtac tggtcgagct tctggcggaa gccgaggagc
      481 tgctggatca ggcgctcggc ggccccgggg gcgttgcggc gggcccgcag ggtgtcgtgc
      541 atgtgcttgt ggccggggac gagccggtcc ccgagctggt ccatgacgaa ctcgccgtgg
      601 ccctcgagca cggtcatggt cgactgcacc cggtcgacga tggtcttctg ctcgggggtg
      661 acgagcaggt cgagcagctc gatcttctcg ccggcgatga cccgccgggc caggccctgg
      721 agccgctggg agaggcgctt gagggcctcg tcgtccacct ccaccgaccc gaggtactcg
      781 tccagcaggc cctggatgtg cccgggcagc cagggcacgc cccggaactg gcggcggtgg
      841 gcggtctcgt gcagggcgat ccagaggcgg aagtcggccg ggcggaagcg gtggcggcgc
      901 tcggtggcga cgatgttggg caccacgtac cagaccgtcc cctcgccgtg ggccttcacc
      961 ggcagcgggg cgtcgccgtt gggggagacg gcctggtcgg ggccgggcat gggcaggtcg
     1021 tactggccga ggacgcgctg gccgacgtag ccgagcaggg tgccggcctg ggccccggcg
     1081 atcgccctgg tgctggtgcg catggccgcc ggcaccgggc cggtggcctt ccaggccttg
     1141 cggtcggcca ccttctcggc cagcggccgc aggatccggc ggagggtggc caggttggcc
     1201 tcgacccact cgccgcggtc gagcggctcg gcgatcggcg gcggcagctc cccgccgagg
     1261 ccggtgaact cggccacggc ccggtcggcg gtggtcgaca ggtcggtgaa ctggcgcgac
     1321 caggcggcgc ggacgggcag ctccgaccca ccggccaccc ggcggccgac ccgggccgcc
     1381 agctgccagt cgacgagctc ggggacgtcg gtgggaaggg cggtcgagac cacggtagct
     1441 ccaggaacga ggcggcgtgc cttcccaggc tacgccagca ggcgcggttc ggcggaggcc
     1501 gggggcgtca ggacttgggg cggctgaagc cgggccagaa gcgcacgtac agcgcgatca
     1561 ggaccagggc gaggagcgcc ccgagggcga agagccccca gccggcccag tagatggccg
     1621 ggcggcccga ggacggcggg gcggccgcgc tcggggtctc gaccacggcc ccgccggcct
     1681 ccccctgggt ggtggtggtc gcggccgcgg ccgtggtgcc gaccgtgatc gtgcccttca
     1741 tgccctgggt gacgtggacc tggcagaagt aggggaaggt cccggccttg gggaacttca
     1801 cctggcagga gtcgccgacc ttggtcatgt tggcgatggt gcagtcggag gcgtcgaatt
     1861 tgccgtcgct cttctcgatg tcggacccgt ctccggagaa caccgggtgg gtggtgccgc
     1921 cggtgatctc gaaggtgacc gtgccgcccg gccggacggc ggcgtcggcc ggcacccacg
     1981 tgttctcggc ggtcccctcg atggtgacct ggttcgggac ggactgcgcc tcggccgggc
     2041 ccccggcggt caggaccagc ccggccccga gcagggcgac ggcgatggcc agtgggagga
     2101 ggcggggcag gctgcgggcg acggcgaggt gcatgggctg cgttgcgctc ctgtcagggg
     2161 tccggggcct cgagcctagc ccccggccgg tcgaatcgtt cggctaggcg gccggattgt
     2221 agcaagcgcg acccaggtca ggggcccgtg cccgccgcgc ccgcaacccc ctctgccgct
     2281 gctcctcgcc gctgtcgctc gccggggcgc tggtgcacaa tcctcgcgtg aacagagccg
     2341 gaggggagcg gggggcgacc gccgtcgcct acgcggtgat ggtcgcgatc atcgcgttgc
     2401 tcctggtcgc cggggtgttc gtgctcggcg gcagcatcga gcaggccttc atcggcggag
     2461 ccgactgtat cgtcagcccc gggagctgcg acgacggttc ggggggcggc ggcggtggtg
     2521 gcggtggtgg tggcggcggc gggggcggcg gcggcggggg cggcggcccc acgacgacca
     2581 acccgcccgg accgaccacg accacgacca cgaccggcgg cccctgaccc ggctactccc
     2641 gggccaggcg gcgcaggccg gtggtggcca cctgctggag gctctccagc tgggcggcca
     2701 gaaccttggt ccccttctcg gacagccggt aggggcgccg gcggtcgtcg gccggcaggg
     2761 cctcgatcag cccctggtgc tggagccggg ccagggcgcc gtacagcgtc cccgggccca
     2821 gggtgacccc cgcgaaccgc tggatgtcct tggtcatggc gtagccgtgc ttggggccgt
     2881 cggccaggct cgacatgacg agcaacgacg ggtcagagaa ccgcccgtgg tcaccgaggt
     2941 cgccaggcgc catctaccct cccccatagg gccggcgatc ccagtgtccg ggccccaccg
     3001 accacggtca agcgcccgcg gaagaaccgg cgaatcctgc tacctttccc ggcagggccg
     3061 gggtttgacg gagggccccg gcggtcaatc tggcggcggc aaccgccgtc gacgtctcca
     3121 ggcgaggtag gaccatgaag atcaggaaac ggctggctca gctcgggctc atcatggtgg
     3181 cgctgcccgt ggtcggctgg ggcctcgagc agctggccaa gcgggccgag gagcgccggc
     3241 ccgggtcggg caagtggctg cgcacggggt cggacttcgc ccacgggttc ggccgcggcc
     3301 cgctctcctc ccggctccgc accccccgcc ggtagcccac cggggtgata cggtgcgggc
     3361 atgaggaccg caccacgcat cggctcggaa ctggtggggt ggcgccggct ggcggtcgtc
     3421 gcggcgctgg cgaacgcggt ggtcctgttc ggctacggcc tcacccgggg tgaccgcgag
     3481 gccctcgtcc tcgccgtggt catggtggtc ggcctcggcc tgctgcgggt cggcaccggg
     3541 ctgctcggcc tgctggtgct ggcggcgctg ttcgtgaaca tcgagatctg gatgttcccg
     3601 gcggccaacg gcaacgcgac ctaccgggcc gggctgctcg acctgctggt ccccgcgtcg
     3661 ctggtcgcct tctcgctggc cggcttcatc ggggcggtgg ccgccctggt ccacctgcgc
     3721 gacccggcgg cgggacgggg cgcggccggt cccaccggcg tgatcgccgt gctcttcatc
     3781 ttcatcagcc tcgggctggt gtgggccatg accgacgccg agccccaggt cgtccccgag
     3841 ggggcggtcg aggtccagac gaccaacgtg gccttcgtcc ccgacacgct ccgggcgggt
     3901 ggcggccagg tgacggtggc ggtccgcaac cgcgacctgt tctggcacac cttcaccatc
     3961 gacgccctcg gcgtcgacat cagggcgccg gtcgggggcg tgcgctcggc cagcttcgag
     4021 gccgagccgg gcacctacga cttctactgc cgcatccccg ggcacgccgc cctgggaatg
     4081 cgcggcaagc tcaccgtcgg ctgacccgga cggcggcccg gggtcagatc gagtcgtaga
     4141 tggtcacgaa cccgtagccg cgccgcttga gctcggcgat gatccccggc agggcgtcgg
     4201 cgtccagggt ggagccgtcg gtggggtggg agccgacgtg catgaggacg atctcccccg
     4261 ggcggaggct ggccagcacc cggttcgtca ccgactggac gctctggccg ccggaggtgc
     4321 cctgccagcc gagcgagtcg acggtccagc ggatcgaccc gtagcggagc ccgttgacga
     4381 tcgccagggt ccgggcgtcg cggtcgccga aggggaagcg gaacatcggc cgggcgttga
     4441 acttggtcgc cccctggatc ttgcgctcgg cccggttgat ctcgtcgcgc acggccgcgt
     4501 ccgacagggc gggcaggtgc gggtgggtgt cgctgtggtt gccgaccggg tagcggaccg
     4561 cgatccggcg ggtgtcggcc gggaagttgt cgacccacct gccggtgagg aagaaggtcg
     4621 ccgggacgca gttggcctcg agggtggaga ggatcgaggg cacggcgctg gcgttggcgc
     4681 cggcgtcgaa ggtcagggcg accacgttct gggtggtggc caccgtgttg agctcggtcc
     4741 cccgcagggc ggcgggcagg cccttgccgc cgcgaccccg ggtgatcgcc cccggcccgg
     4801 cgaagccgag gtcgcagccg aggccgtagc tgaaggtgag gtcggccggg ccggcctggt
     4861 tgctgttgcg cagggtccag gcgctgcgcc gcttgacgcc gacggtggtg gtgccgttgc
     4921 cgtcccagtc gccgacgacc cgctggtcgg aggggaggcc gaagtcgaag ggggggacgg
     4981 tggtggtgtt ggccgggtcg ttggtggccc gcaggaacca ggtggcgccg cggaccacgc
     5041 ccggggtcga ggtgccgtcg ccgttccagt ccccggccac ggccaggtcg gaggcgaacc
     5101 cgtaggcgaa gctgacgtcg gccacgccgg ggctggcgtt ggtgttcttc aggaaccagg
     5161 tgctgccccg gaccaccccg ggggtgtcgg tgccgttgcc gtcccagtcg ccgaccaccg
     5221 ggtagtcccc cgggaacccg taggagaagc tgacgtcggc cggcccggcg ctgaggctgt
     5281 tgcgcagcag ccaggacgag ccccggacca cgccgggggt ggcctccccg tcgccgtccc
     5341 agtcaccgag cagggggaag tcgcccgcct ggccgtgggt gaaggtgtgg tcggccaccc
     5401 cgccggcgag ggtgtcgcgc acgaaccagg tgttccccct gacgatcacc ggcttggtcg
     5461 cggcccccac ctgcaccgcg gccccggcgc cggtcccggc gacccgggct ccggcggcgc
     5521 cggccccggc cgccatctg
//
LOCUS       DASSWR010000720         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1411035, whole genome shotgun sequence.
ACCESSION   DASSWR010000720 DASSWR010000000
VERSION     DASSWR010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411035"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1107)
                     /locus_tag="VFO47_12115"
     CDS             complement(127..1107)
                     /locus_tag="VFO47_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8789481.1"
                     /translation="MIRLINALLAIVGGVGAALLLFWILNKLVEMLPLKWEERLKPWV
                     FIGPAVAAIAFFLIYPAIRTFILSFANAQSTAWVGTENYTDLLSSSEFRNTLYNTLLW
                     IAVVPAATVALGLGVAVLADRLGPQSEKLSKSIIFLPMAISGVGQATIWRFIYEARPE
                     GEPQIGLQNAVWTALGFDPVAWLQVSTLHFNSFLLMIILIWAQVGFSMVLLSAAVKGV
                     PVDTLEAARIDGANERQIFFRVIVPQIWATVITVFITVLISVMKIFDVVYVMTNGNFN
                     TDVIAVRFFNELFRNGNNGRASAIVVMLMIAVLPVIVYQVRHFRAEEAAR"
     gene            complement(1199..2518)
                     /locus_tag="VFO47_12120"
     CDS             complement(1199..2518)
                     /locus_tag="VFO47_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8789482.1"
                     /translation="MRWRRLFQGIAVAAVAMLFATACLEEGGGGGGGGGSAAEGDGQV
                     EILFGFGGDQSKGFQDGLNQWASANGVTIKYSEASQSFDTIVRTRVQGNNLPDIALFP
                     QPGVMMDIANSGKMQDLSGVIDKAKVESTLVPGELAAGTSADGKLYGIPMSMNVKSLV
                     FYPKKAFEAKGYTVPTTVAELEALTNQIKSDGTTPWCVGIESAAATGWPATDWIEDFV
                     LRYGGPEKYDQWVKHEIPFNDGLVVQAAQEFEKLALADGNVFGGRKAVVSNAFATAGN
                     PMFQDPPKCFLHRQGNFITQKDFFPDKVRANLDEEVGVFQLPGTDAANKPVLGGGDLA
                     GAFSNDEDTKKVMAHLTSPDFEFADLAASSWISPHKTFDVSKYPDETTKKIAELAYQS
                     TVFRFDGSDQMPGAVGAGSFWKGMTAWISGQQDLDEALTSIEESWPS"
BASE COUNT          415 a          883 c          870 g          460 t
ORIGIN      
        1 cgagcctgac cacccagttg gcctgcccgg cgccgggagc ggccccgccc tcgcggcgag
       61 ggatggcccg gcgcggcttg acctcgccgg ggaccggggt cggaggagga gtggttccgg
      121 tggtcgtcat cgtgctgcct cctcggcccg gaagtgacgc acctggtaga cgataacggg
      181 cagcacggcg atcatcagca tgaccacgat ggccgaggcc cggccgttgt tgccgttgcg
      241 gaacagctcg ttgaagaacc ggaccgcgat cacgtcggtg ttgaagttgc cgttcgtcat
      301 cacgtagacg acgtcgaaga tcttcatgac gctgatgagc acggtgatga acaccgtgat
      361 caccgtggcc cagatctgcg ggacgatcac ccggaagaag atctggcgct cgttggcccc
      421 gtcgatacgg gccgcctcca gggtgtcgac gggcaccccc ttgacggcgg cggagagcag
      481 caccatcgag aacccgacct gggcccagat caggatgatc atcagcagga agctgttgaa
      541 gtgcagcgtg ctgacctgca gccaggccac cgggtcgaac ccaagggcgg tccagacggc
      601 gttctggagg ccgatctggg gctcgccttc ggggcgggcc tcgtagatga accgccagat
      661 ggtggcctgg ccgaccccgc tgatggccat gggcaggaag atgatcgact tgctgagctt
      721 ctccgactgg ggtcccagcc ggtcggccag gaccgccacg ccgagcccca gcgccaccgt
      781 ggccgccggg accaccgcga tccacagcag ggtgttgtac agcgtgttcc ggaactcact
      841 ggaactgagc aggtcggtgt agttctcggt gccgacccag gccgtgctct gggcgttggc
      901 gaagctgagg atgaacgtcc ggatggccgg atagatgagg aagaaggcga tcgccgccac
      961 cgccggcccg atgaacaccc agggcttgag gcgctcctcc cacttcaggg gcagcatctc
     1021 gaccagcttg ttgaggatcc agaacagcag cagggcggcg cccacgccac cgacgatcgc
     1081 cagcaaggcg ttgatcagcc tgatcatcga cttgctcctt tctgctcatg gcgccgccgc
     1141 gccgcccggt tggtgggtgc gcggtccgtg ccggccgcgc acccaccatc agcgatctct
     1201 agctcggcca gctctcctcg atggacgtca gggcctcgtc caggtcctgc tgcccgctga
     1261 tccaggccgt catgcccttc cagaagctgc cggcacccac ggcgcccggc atctggtcgg
     1321 agccgtcgaa ccggaacacg gtcgactggt aggcgagctc ggcgatcttc ttggtggtct
     1381 cgtccgggta cttgctcaca tcgaaggtct tgtgcggcga gatccagctc gacgcggcca
     1441 ggtccgcgaa ctcgaagtcg gggctggtca ggtgcgccat gaccttcttg gtgtcctcgt
     1501 cgttgctgaa ggcgcccgcg aggtcgccac cgccgaggac cggcttgttg gccgcgtccg
     1561 tgcccggcag ctggaacacg ccgacctctt cgtccaagtt ggcccggacc ttgtcaggga
     1621 agaagtcctt ctgggtgatg aagttgccct gccggtgcag gaagcacttg ggcgggtcct
     1681 ggaacatggg gttgcccgcg gtggcgaagg cgttgctcac gaccgccttg cggccgccga
     1741 agacgttgcc gtcggccagg gcgagcttct cgaactcctg agcggcctgg acaaccaacc
     1801 cgtcgttgaa cgggatctcg tgcttcaccc actggtcgta cttctcgggg ccgccgtagc
     1861 ggagcacgaa gtcctcgatc cagtcggtgg ccggccaacc ggtcgccgcg gcggactcga
     1921 tgcccacgca ccacggggtc gtgccgtccg acttgatctg gttggtgagc gcctccagct
     1981 cggcgaccgt ggtcggaacc gtgtagccct tggcctcgaa ggccttcttc gggtagaaga
     2041 ccaggctctt gacgttcatg ctcatgggaa tgccgtagag cttgccgtcc gccgaggtcc
     2101 ccgcggccag ctcaccgggg acgagggtcg actcgacctt ggccttgtcg atcacgccgc
     2161 tcaggtcctg catcttgccg ctgttggcga tgtccatcat cacgccgggc tgcgggaaca
     2221 gggcgatgtc gggaaggttg ttgccctgca cgcgggtccg gacgatggtg tcgaaggact
     2281 gcgaggcctc ggagtacttg atggtgaccc cgttggcgct cgcccactgg ttcagcccgt
     2341 cctggaagcc cttggactgg tcgccgccga agccgaacag gatctcgacc tggccgtcgc
     2401 cctcggccgc cgagccgccg ccaccgccac cgccgccgcc ttcctcgagg caggccgtag
     2461 cgaacagcat cgccaccgcc gccaccgcga tcccctggaa caatctacgc catcgcatct
     2521 tgtctccctt tcgttgacgc cgcagcacct gtcggtagac ggtcttccct cacgggcctg
     2581 cccctcccgg gtctgcaacc gctacctggc gtgctggccc accagctt
//
LOCUS       DASSWR010000721         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_761325,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000721 DASSWR010000000
VERSION     DASSWR010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761325"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFO47_12125"
     CDS             <1..249
                     /locus_tag="VFO47_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8789483.1"
                     /translation="IRTMAAKGIPGFTLLADAWSKRAPLGWDVGDPTVVARVALWLFS
                     DWSRGVCGELIHVDGGFHAMGTDLLSKEEQARLAAEDG"
     gene            342..2018
                     /locus_tag="VFO47_12130"
     CDS             342..2018
                     /locus_tag="VFO47_12130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8789484.1"
                     /translation="MIFVVADDGRLVDALAADLGRRFGGDYRIVAERSPAGALATLER
                     LAGTGELVALVIAARRMEGLDGLALLLRARELHPSAKRVLLEHRGEWTSGQPVVRAMT
                     LGQLDYVLFRPWLPIEQCLYLPVTEFLAAWEKSRGEGAEAIQIVGRRWAARSHELRDT
                     LARVGIPFGFYPDDSPDGRRLLEQAGEDGSRLPVVLFRRGLVLVDPTHAELTRALGMR
                     TSPAGGGCDVLIVGAGPAGLAAAVYAASEGFAAQVLEPAVPGGQAGTSSHIRNYLGFP
                     YGLSGDELAQRAVQQAWLFGADFILAQAATGLRARGRDRLVRLSDGGEVATRAVILAM
                     GVAWRRLGVPALEALNGAGVFYGSTGGEARALRGEDVFVVGAGNSAGQAAIHLSAYAA
                     SVTIVTIDERLGEFMSDYLVRKVEATPNIAVLLHTEVVDGHGRQRLEGLTLRDRHTGA
                     TREVPASAMFVLIGAEPRTDWLEGTVERDERGYLLTGRDLTGADGRSPAGWPLERQPL
                     PLETSLPGVFAVGDVRYRSVKRVASAVGEGSIAVQLIHEYLADTQDPARR"
BASE COUNT          230 a          731 c          810 g          280 t
ORIGIN      
        1 atccgcacca tggccgccaa gggcatcccc gggttcaccc tgctggccga cgcctggtcc
       61 aagcgggcgc cgctcggctg ggacgtcggc gacccgacgg tggtcgcccg ggtcgccctg
      121 tggctgttct cggactggtc ccggggcgtg tgcggcgagc tgatccacgt cgacggcggc
      181 ttccacgcca tgggcaccga cctcctgtcc aaggaggagc aggccaggct ggcggccgag
      241 gacggctaag gaagccaccc ctcgccatgg cagggtcgga ggttctgggg cgccgtgggg
      301 ggagcgggct ccccccacat ggatcaggtt cgcagcggcc ggtgatcttc gtcgtcgccg
      361 atgacggccg cctggtggac gcgctcgccg ccgacctggg gcggcgcttc gggggggact
      421 accggatcgt ggccgagcgc tcccccgccg gcgcgctggc caccctggag cggctcgcgg
      481 gcaccggcga gctggtcgcc ctggtgatcg cggccaggcg catggagggc ctggacgggc
      541 tggcgctgct gctgcgggcc cgggagctgc acccgtcggc caagcgggtc ctgctggagc
      601 accggggcga gtggacctcg ggccagccgg tggtccgggc catgaccctc ggccagctcg
      661 actacgtcct gttccggccc tggctgccca tcgagcagtg cctgtacctg ccggtgaccg
      721 agttcctggc cgcctgggag aagtcgcggg gggagggtgc cgaggcgatc cagatcgtcg
      781 gccggcgctg ggcggcccgc tcccacgagc tgcgcgacac cctggccagg gtcgggatcc
      841 ccttcggttt ctaccccgac gactcgcccg acggccggcg cctgctcgag caggccggcg
      901 aggacggcag ccgcctgccg gtggtgctgt tccgccgcgg cctggtcctg gtcgacccga
      961 cccacgccga gctcacccgg gccctgggca tgcggaccag cccggccggt ggcggctgcg
     1021 acgtgctgat cgtcggggcg gggccggccg ggctggccgc ggccgtctac gccgcctccg
     1081 agggcttcgc ggcccaggtg ctggagccgg ccgtccccgg aggccaggcc ggcaccagct
     1141 cccacatccg caactacctg gggttcccct acgggctgag cggcgacgag ctggcccagc
     1201 gggccgtgca gcaggcctgg ctgttcgggg ccgacttcat cctggcccag gccgcgaccg
     1261 ggctgcgggc caggggacgc gaccggctgg tgcggctgtc ggacggcggc gaggtggcca
     1321 cccgggcggt gatcctggcc atgggcgtgg cctggcggcg gctgggcgtg ccggcgctgg
     1381 aggcgctgaa cggggccggc gtcttctacg ggtccaccgg tggcgaggcc cgggcgctgc
     1441 ggggcgagga cgtgttcgtg gtcggcgccg gcaactcggc cgggcaggcg gcgatccacc
     1501 tgtccgccta cgcggcctcg gtgacgatcg tcaccatcga cgagcggctg ggcgagttca
     1561 tgtccgacta cctggtgcgg aaggtcgagg cgaccccgaa catcgccgtg ctgctccaca
     1621 ccgaggtggt cgacggccac ggacgccagc gcctggaggg cctgaccctg cgggaccggc
     1681 acacgggcgc gacccgggag gtccccgcct cggcgatgtt cgtcctgatc ggggccgagc
     1741 cgcgcaccga ctggctggag gggacggtgg agcgcgacga gcgcggctac ctgctcaccg
     1801 gccgcgacct gaccggtgcg gacggccggt cgccggccgg ctggccgctg gagcgccagc
     1861 ccctgccgct ggagaccagc cttcccgggg tgttcgccgt cggcgacgtc cgctaccgct
     1921 cggtcaagcg ggtcgcctcg gccgtgggcg aggggtcgat cgccgtccag ctgatccacg
     1981 agtacctggc cgacacccag gaccctgccc ggaggtaggt ggtggcgggc ggcttctcgg
     2041 aggcggtgtg g
//
LOCUS       DASSWR010000722         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_959281,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000722 DASSWR010000000
VERSION     DASSWR010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959281"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1816)
                     /locus_tag="VFO47_12135"
     CDS             complement(80..1816)
                     /locus_tag="VFO47_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8789485.1"
                     /translation="MGPYWRRVAVGFGLGVAMLAITSFIPLVTKTIIDEGLTQRVPGV
                     LGREIALLVVLSVVRWVVGGLRRNLSGRVGTDVENDLRNRLARHLLALEPAYHDRAPT
                     GQLLARSTSDVRSLRYFISWGLVFLVLNIVTFLIAATQMWLLAPRLCLVVLVLSPPLV
                     YGALRFNRRLHRVYWQVQQEVGELTTVVEEATAGVRVVKAFGREREQARRLEVAASSI
                     LDHNLEAARIRSFYVPLLAVLPQLSLAAILWYGGRLAIDGQITLGTLVAFNSYLLLLT
                     WPLQGLGMLFGFAQRAAASAERVFEVLDQSPAIADRPGATTLAAGPGRRGARVRFEHV
                     RFGYGEGGRAALEGIDLDIASGSRVAVVGGTGSGKSTLLGLVPRLYAPTEGRVLVDGH
                     DVADVTLDSLRAAVGIVHQEPVLFSASLRDNIAFGRPQATDEEVLGALAAAAALEVVE
                     ALPEGLDTVVGEQGYTLSGGQRQRVALARALLMEPRVLILDDALSHVDVDTEAAILAG
                     LDAAVGGATVLLVANRPASLRLAERVVLLDRGRVVAQGRHEELAAREPAYRAVLAHAG
                     GGVDRLVEEAAS"
BASE COUNT          293 a          773 c          749 g          224 t
ORIGIN      
        1 ccatcggccc cccgcgcagt cccgcgcctc gtcttctact caccggggca ccccctcggg
       61 ttcccggacc ctccggtgct catgacgccg cctcctcgac caggcggtcg acgccgccgc
      121 cggcgtgggc gagcacggcc cggtaggcgg gctcgcgggc ggccagctcc tcgtggcggc
      181 cctgggcgac cacccggccc cggtcgagca gcaccacccg ctcggccagg cggaggctgg
      241 ccggccggtt ggccaccagc agcacggtgg cgccgccaac ggcggcgtcg agcccggcca
      301 ggatggcggc ctcggtgtcg acgtcgacgt gggagagggc gtcgtccagg atcagcaccc
      361 gcggctccat cagcagggcc ctggccaggg cgacccgctg gcgctggccc ccggacagcg
      421 tgtagccctg ctcgccgacc accgtgtcca gcccctcggg cagggcctcg accacctcga
      481 gggcggccgc cgcggccagc gcgcccagca cctcctcgtc ggtcgcctgc gggcgcccga
      541 aggcgatgtt gtcgcggagg ctggccgaga acagcaccgg ctcctggtgg acgatcccca
      601 ccgccgcccg tagcgagtcc agggtcacgt cggcgacgtc gtggccgtcg accaggaccc
      661 gtccctcggt gggcgcgtac agccgcggca ccagcccgag cagggtcgac ttgcccgagc
      721 ccgtgccccc gaccaccgcc acccgcgacc ccgaggcgat gtcgaggtcg atcccctcca
      781 gggcggcccg gcccccctcg ccgtagccga agcggacgtg ctcgaaccgg acccgcgccc
      841 cccgccgccc cggcccggcc gccagggtgg tcgcgccggg ccggtcggcg atggccgggg
      901 actggtcgag cacctcgaag acccgctcgg cgctggccgc cgcccgctgg gcgaagccga
      961 acagcatccc gagcccctgc agcggccagg tgagcagcag caggtagctg ttgaaggcga
     1021 ccagcgtgcc cagggtgatc tggccgtcga tggccagccg gcccccgtac cagaggatcg
     1081 cggccaggct gagctggggc aggacggcca gcagggggac gtagaaggag cggatccgcg
     1141 ccgcctccag gttgtggtcg aggatcgagc tggccgcgac ctccaggcgc cgggcctgct
     1201 cccgctcccg cccgaacgcc ttgaccaccc ggaccccggc cgtggcctcc tcgaccacgg
     1261 tggtcagctc gccgacctcc tgctgcacct gccagtagac gcggtgcagg cggcggttga
     1321 agcgcagcgc cccgtagacc agcggcggcg acagcacgag cacgaccagg cagagccggg
     1381 gcgcgagcag ccacatctgg gtggccgcga tcaggaaggt gacgatgttg aggaccagga
     1441 agaccaggcc ccaggagatg aagtagcgca gcgagcgcac gtccgaggtg gacctggcca
     1501 gcagctgccc ggtcggggcc cggtcgtggt aggcgggctc gagggccagc aggtggcggg
     1561 ccaggcggtt gcgcaggtcg ttctcgacgt cggtgccgac ccggccggag aggttgcgcc
     1621 gcagcccgcc gacgacccag cggacgacgg acaggaccac cagcagggcg atctcccggc
     1681 ccagcacccc cggcacccgc tgggtcagcc cctcgtcgat gatcgtcttg gtcaccaggg
     1741 ggatgaagga ggtgatggcc agcatggcga ccccgagccc gaacccgacg gcgacccggc
     1801 gccagtaggg ccccatcagg cccacgatgc gccgcaggct cccgagggcg aggccgtcgg
     1861 gaggcggttc ggttccgtac cagcgcagcg ggcagctcca gcagttcgag ggcgggtgtc
     1921 ctcggtccaa cgtacgggag cccccagaca atccgcgacc ggccatccac agcccgcccg
     1981 ggggcgggtt cgggtcaggc gggcagctgc aggaccgcgt tcggcgaggt ggtggacac
//
LOCUS       DASSWR010000723         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_224804,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000723 DASSWR010000000
VERSION     DASSWR010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224804"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1716
                     /locus_tag="VFO47_12140"
     CDS             235..1716
                     /locus_tag="VFO47_12140"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HET8789486.1"
                     /translation="MEEIARVLTRHPPSDRLPAAVPAQMTAQPDLLSVQVETLASRPI
                     VTCAPDDTVTQAARRMRDQLVSSLVVDSRPPGLVTATDLRDRVLAAGRDPATPVCQVM
                     TTPLHTIPADSTIGEVVLAMVDRGIHHLPLTRQGRLVGIVSDTDLLRRESHHPLLVRR
                     QLDRAAGPEELAAYAREVTTAAARLVGVGTPPAEVTRFLTSANDALYVRAARDGEAAL
                     GPPPCPYALLVLGSGARRESTLRTDQDHALVLADGAAPGADGWFAALAEHLAATLEQC
                     GLVRCPGNIMATNPARRVPLGAWQEQFIRWIEEPEEDAVLAAATYFDFRQLHGELDAE
                     APLRRIAGQAAGNRRFLGRLAVAALWRRPPLGFLHHLRGDHHGRIDLKAHGTAPIVDL
                     ARLLALDAGDPAVATTARLQAAADHGTAGTAATDLAAAFEYLQQLRLRHQADRLAAGA
                     VPDDAVSLAELTALQRRWLKDTAELLRTCQESIRIAYRTDLIG"
     gene            complement(1740..>2284)
                     /locus_tag="VFO47_12145"
     CDS             complement(1740..>2284)
                     /locus_tag="VFO47_12145"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8789487.1"
                     /translation="TGADRGDFQPWWPPITAPNMTAQGGASAMARIDGELVIDRPVHE
                     VFDFVADERNEPRYNPRICRAEKLSPGPIGRGTRFRAEAVTLGQTTGMTVEYTTYERP
                     RRLASSIHMPAADIAGTLRFDPVANGTRMGWSWVVRPRGPYRLLTPVIVGVGRRQERE
                     NWGGLKRFLEARKELAPAAP"
BASE COUNT          305 a          892 c          770 g          317 t
ORIGIN      
        1 gaccacgacc tcgtcgttgg tgctcatggc cagccgcttg gccagctcga ccgccttctc
       61 ggagtacttg gacccgtcga cggcgagcag gatggtccgg aacatcgggt gcctcctctc
      121 gaggtgtgcc tccagcatcg atgcaggggg cgccgccacc cagtgccgga cggcccctat
      181 ccgacaggcc gtacgaccgg agccaacccg gctagacacg acgatgctgt atccatggag
      241 gagatcgccc gggtgctcac ccgacatccg ccgtccgatc ggctcccggc agccgtgccg
      301 gcgcagatga cggcgcaacc ggacctgctc tcggtccagg tcgagacgct ggccagccgg
      361 ccgatcgtca cctgcgcccc ggacgacacg gtcacccagg cggcccggcg catgcgcgac
      421 cagctggtgt cgtcgctggt cgtcgacagc cggccgcctg gcctggtgac cgccaccgac
      481 cttcgcgacc gcgtgctcgc ggcaggccgt gatcccgcca caccggtatg ccaggtcatg
      541 accacgccgc tgcacaccat cccggccgac agcaccatcg gcgaggtggt cctcgccatg
      601 gtcgaccgcg gcatccacca cctgcccctg acccggcagg gacggctggt cgggatcgtc
      661 tccgacaccg acctgctgcg gcgggagtcc caccatcccc tgctcgtccg ccgccagctc
      721 gaccgggcgg ccggccccga ggagctggct gcctacgccc gtgaggtgac cacggccgcg
      781 gcccgactgg tcggggtcgg cactccaccg gctgaggtca cccggttcct gaccagcgcc
      841 aacgatgccc tctatgtccg cgccgcccgt gacggggagg cggcgctcgg gccgcccccg
      901 tgcccgtacg cgctcctcgt cctgggcagc ggggcccgcc gggagagcac gctccgcacc
      961 gaccaggacc acgccctggt gctggccgat ggcgccgctc cgggggccga tggctggttc
     1021 gccgccctgg ccgagcacct ggcggccacg ctggagcagt gcgggctcgt ccgctgcccg
     1081 ggcaacatca tggccaccaa cccggcccgg cgggtcccgc tgggagcctg gcaggagcag
     1141 ttcatccgct ggatcgagga gccggaggag gacgcggtgc tcgccgcggc cacctacttc
     1201 gacttccgcc agctccatgg cgagctcgac gccgaggcgc cgcttcggcg gatcgccggc
     1261 caggccgccg gcaaccggcg gttcctcggc cgcctggcgg tggcggccct ctggcggcgc
     1321 ccaccgctcg ggttcctgca ccacctgcgc ggcgaccacc atggccggat cgacctcaag
     1381 gcgcacggaa ccgcgccgat cgtcgacctg gcccgcctgc tcgccctgga tgccggcgac
     1441 ccggcggtgg ccaccaccgc tcggctgcag gcggcggccg accacggcac ggccgggacc
     1501 gccgccaccg acctcgccgc cgccttcgag tacctgcagc agctgcgcct gcgccaccag
     1561 gccgatcgac tggccgcggg agcggtcccg gacgatgccg tctccctggc cgagctcacc
     1621 gcgctccagc gccgctggct caaggacacg gctgagcttc ttcgcacctg ccaggagagc
     1681 atccggatcg cctaccggac cgacctgatc ggctgacctc ctgccgtggc ggctgcgagt
     1741 cagggggccg ccggcgccag ttccttccgc gcctccagga accgcttcag gccaccccag
     1801 ttctcccgct cctgacgccg gcccacaccg acgatgaccg gggtcagcag cctgtacggc
     1861 ccacggggcc tgaccaccca cgaccagccc atccgggtgc cgttcgcgac agggtcgaag
     1921 cgcagggtcc cggcgatgtc cgcggccggc atgtggatcg acgacgccag ccgtcgaggc
     1981 cgctcatagg tcgtgtactc gacggtcatc ccggtggtct ggcccagcgt gacggcctcg
     2041 gcccggaacc gggtcccgcg gccgatgggg ccgggcgaga gcttctcggc ccggcagatg
     2101 cgtgggttgt accgcggctc gtttcgttcg tcggcgacga agtcgaacac ctcatggact
     2161 ggccgatcga tgacaagctc gccgtctatc cgtgccatgg cggatgcacc tccctgtgcc
     2221 gtcatgttgg gcgccgtgat gggcggccac cagggctgga agtccccccg gtctgcgccc
     2281 gttc
//
LOCUS       DASSWR010000724         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_732609,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000724 DASSWR010000000
VERSION     DASSWR010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732609"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..778
                     /locus_tag="VFO47_12150"
     CDS             101..778
                     /locus_tag="VFO47_12150"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET8789488.1"
                     /translation="MPTPPATEQPGSPEDLVRALRAEGIRDRRVLAAFRAVPRAGFVP
                     PEAAGQAYLDAPIRIPHGQVTTQPSLIAAMVAALELTGTERVLEVGTGLGFQTAILAM
                     LARQVVSVERFPDLAAQARANLAAAGLAGATVVVGDGTLGVPGHAPYEAIVVAAAAPH
                     VPGPLIDQLAPGGRLVHPVGPGGREQVTAFRQQAGRLVTDAQLVPARFVPLIGVHGVS
                     SRQDRGG"
     gene            778..1737
                     /locus_tag="VFO47_12155"
     CDS             778..1737
                     /locus_tag="VFO47_12155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET8789489.1"
                     /translation="MTVTVALAGDTMLGRGVARALTASPPQALVAPEVRAAMDQADLV
                     VLNLECCISERGRPWDAPGKPFFFRAPPRAVELLVLLGVDCVTLANNHALDYGVDALL
                     DTLVHLDAAGIAVVGAGADLEQARQPAVLGARGLRVAVVGVTDHPPDFSAGPDRPGVA
                     FADLGRAVPDWLLQTVRAADADVLLVSPHWGPNMTTAPVRHVRRAATALVDAGATLVA
                     GHSAHVPHGVAGRVLYDLGDFLDDYRVDPRLRNDLGLLFLVSLDAGGPVRLEALPLKL
                     DFCHTRLAAGEDAAWMRHRFRAACATLGTTVEQATDRLIVSWR"
     gene            2113..2847
                     /locus_tag="VFO47_12160"
     CDS             2113..2847
                     /locus_tag="VFO47_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8789490.1"
                     /translation="MPAAIPAADRLAAQLTTAETAIRDPATPAGRLPALGRAQQRAYR
                     TLVRQPRLVPKVLGQLSPSLRRVVQANVLAGRELRKLNRPAGQLPRWRIVAPAPAGRL
                     LAAYRAAQAKLAVPWEYLAAIHLVETRMGRIRGTSSAGAQGPMQFLPSTWKRYGHGGD
                     IGATGDAIVAAARMLRANGAPADMAAALYAYNPSRRYVRAVSAYASLLRANRRAFLGY
                     YHWQVFYGDTLLPEGYPARPPVPAPG"
     gene            complement(2854..>3257)
                     /locus_tag="VFO47_12165"
     CDS             complement(2854..>3257)
                     /locus_tag="VFO47_12165"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8789491.1"
                     /translation="LVSVLRWPLLAAVLIAGLAVLYRVAPSRKDARWQWLSWGSLAAT
                     GLLLVGSMLSSFYASYAPAQNKTYGAFFGVIVLLFWLFLNAFAVLLGAELNAELELQT
                     RRDTTVGPPQPLGERGAYVADHVAESPDAPA"
BASE COUNT          439 a         1292 c         1095 g          431 t
ORIGIN      
        1 gtcaaccgga tcagccgcca ggggtcgacc gcctagcccc aagcgcgtcc atcgagccca
       61 gtcatcccgg tcggacggcg caactaggcc aggactcgcc atgccaacac cgcctgccac
      121 cgagcagccg ggatcaccgg aggatctggt ccgggcgctg cgcgccgagg ggatccggga
      181 ccgccgggtc ctggccgcgt tccgggccgt cccgcgggcc gggttcgtgc ctcccgaagc
      241 cgccggccag gcgtacctgg atgcaccgat ccggatcccg cacgggcagg tgaccaccca
      301 gcccagcctg atcgcggcca tggtggcggc gctggaactg accggcaccg agcgggtcct
      361 ggaggtcggc accggactcg ggttccagac cgccatcctg gccatgctgg cccgccaggt
      421 ggtttcggtc gagcgcttcc cggacctggc cgcccaggca cgggccaacc tggccgcggc
      481 cgggctggcc ggggccacgg tcgtggtcgg cgacggcacc ctcggcgtcc ccggacacgc
      541 cccgtatgag gcgatcgtgg tcgcggccgc cgccccccac gtcccggggc cgctcatcga
      601 ccagctcgcc ccaggtggcc ggctggtgca tccggtcggt ccgggcgggc gcgagcaggt
      661 caccgccttc cgccagcaag ccggccgtct ggtcaccgac gcccagctcg tccccgcccg
      721 gttcgtcccg ctcatcggag tccatggggt gtccagccga caggaccggg gtgggtgatg
      781 accgtcacgg tggcgctggc cggggacacc atgctaggtc gcggtgtggc tcgggccctg
      841 accgcctcgc cgcctcaggc cctggtcgcg cccgaggtcc gggccgccat ggaccaggcc
      901 gatctggtcg tgctcaacct ggagtgctgc atctccgagc gtggccggcc ctgggacgct
      961 cccggcaagc cgttcttctt ccgcgcccca ccgcgagcgg tcgagctgct ggtcctgctc
     1021 ggggtcgact gcgtcaccct ggccaacaac cacgccctgg actacggcgt cgacgccctc
     1081 ctcgacaccc tggtgcatct ggacgccgcc ggcatcgctg tggttggggc cggtgccgac
     1141 ctcgagcagg cccgccagcc tgccgtgctg ggggccagag ggctccgggt tgcggtggtc
     1201 ggggtcaccg accatccgcc cgacttcagc gccggccccg accgacccgg ggtcgccttc
     1261 gccgacctcg gccgcgcggt gcccgactgg ctgctacaga cggtacgagc ggccgacgcc
     1321 gacgtcctgc tggtctctcc ccactggggg ccgaacatga ccaccgcgcc ggtccggcac
     1381 gtccgccgcg ccgcgaccgc cctggtggac gcgggcgcga cgctggtcgc cggccactcc
     1441 gcgcatgtgc cccatggggt cgccggccgg gtcctgtacg acctaggcga cttcttggac
     1501 gactaccggg tcgacccgcg actgcgcaat gacctcggcc tgctgttcct ggtctccctg
     1561 gatgcgggtg gcccggtccg gctggaggcg ctccccctga agctggactt ctgccacacc
     1621 cgcctggccg cgggcgagga cgccgcctgg atgcggcatc gcttccgggc cgcctgcgca
     1681 actctcggca ccacggtcga gcaggcaacc gaccgcctga tcgtcagctg gcggtagcac
     1741 ggcgtggggc caccgcccca gagccaggtt gctgtccggc agtccggcca gggcggcaga
     1801 ccgagcggac ccgaagcttc aacgccttgg gcacctagat acaacctggt tggggtccct
     1861 gggtaccctc ggagcatgga cctgcacgcc gacgggcgcg tggccgccga ggtcggccgc
     1921 agccaccgcg accatcgccg tgtcctgcga ctcgccgcct tcctgaccgc tggcttgctg
     1981 gtggctgcgt gctcaggcga tggtcgatca gccgtcagcc caccaccctc cgcccccgat
     2041 cgcaccgcca ccacgatgac caggaccgcc ccagcggccc cgaaggcgcc gacgaccgcc
     2101 ccatccacgg cagtgccggc cgccattccg gccgccgacc ggctggccgc ccagctcacc
     2161 acggccgaga ccgccatccg cgaccccgcc acccccgccg gaaggctccc cgccctgggt
     2221 cgcgcccagc agcgggccta ccgcaccctg gtccgccagc cgcggctggt ccccaaggtc
     2281 cttggtcagc tgtcgccctc gctgcggcgg gtggtccagg ccaacgtgct cgccggcagg
     2341 gaactgcgca agctcaaccg gcccgccggt cagctgccgc gctggcggat cgtcgccccc
     2401 gccccagccg ggcggctgct cgccgcctac cgggcggccc aggccaagct ggccgtgccc
     2461 tgggagtacc tggccgccat ccacctggtg gaaaccagga tgggccggat ccgggggacc
     2521 agctcggccg gcgctcaggg accgatgcag tttctgccct ccacctggaa gcgctacggc
     2581 cacggcggcg acatcggtgc caccggcgat gccatcgtgg ccgcggcccg gatgctacgc
     2641 gccaacggcg cccccgccga catggccgcc gccctgtacg cctacaaccc cagccgccgc
     2701 tatgtgcgcg cggtcagcgc ctacgccagc ctgctgcgcg ccaaccggcg ggcgttcctc
     2761 ggctactacc actggcaggt cttctacggc gacaccctcc tacccgaggg ttacccggcc
     2821 cggccgcccg ttccagcccc cggctgatcg cggtcacgcc ggggcgtccg gtgactcggc
     2881 cacgtggtcg gccacatagg caccccgctc cccgagcggc tggggcgggc cgacagtggt
     2941 gtcccgcctg gtctgcagtt ccagctcggc gttcaactcc gcgcccagca gcaccgcgaa
     3001 cgcattcagg aacagccaga acaacaacac gatgacgccg aagaaggccc cgtaggtctt
     3061 gttctgcgca ggcgcatagc tggcgtagaa cgaggaaagc atcgagccga ccagcagcag
     3121 gccggtcgcc gccagcgaac cccagctcag ccactgccag cgggcgtcct tgcggctcgg
     3181 cgcaacccgg tacagcacgg ccagaccggc gatcaacacc gcggccagca gcggccagcg
     3241 cagcaccgat accagca
//
LOCUS       DASSWR010000725         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_846453,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000725 DASSWR010000000
VERSION     DASSWR010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846453"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFO47_12170"
     CDS             complement(<1..948)
                     /locus_tag="VFO47_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8789492.1"
                     /translation="MTDRIRLGRTDLEVPRLGLGAMTWGSPSGRSRWGPAKLAYGGPP
                     GPEEERRAFEASLTAGVDLFDTAAMYSGGASERRVGELAEGQSVVIATKFPPGWLSTA
                     EAFPEALDQSLARLRRSPIDLYQHHFPSRRISIPSLMGLMADAVEAGKVRAVGVSNYS
                     AAQLRVAHAALAERGIPLASNQVEYSLLHRAPEVNGLLDACQELGITLIAYQPMAQGV
                     LTGKYRPGDRPKGIRRFGRYFRGDGLKKAQPVVALLREIGERHARTPAQVALRWLIER
                     EGVLPIPGAKNGRQAASNAEALSFSLAGDEIQALDQATTA"
     gene            complement(979..2223)
                     /locus_tag="VFO47_12175"
     CDS             complement(979..2223)
                     /locus_tag="VFO47_12175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8789493.1"
                     /translation="MPAWPPSCSGPAGSRPWRWPSGRNEAATDRSRRQGGPVRRRSHD
                     PLDSGLAPAVGDAAVRALADSMTPGLAVALVRHGQIALAAGYGVADQATGEPVTPATR
                     FQAASVSKPVTAWGVLRLVEQGRIDLDEPVVGRLRRWCPPPSPFKTDGITVRRLLSHT
                     AGLSVHGYVGQTLDRPLPAIDASLAGEGGGSFPVELLDAPGRRWLYSGGGYSVLQLLV
                     EELTGRPFADVMQTEVLGPLGMTASSFQWHRTAATARPHDVDGCQVPDFAFAEQAAAG
                     LVTTAPDLARFAAAALPGPEGEPPGRGVLRPAGVRLALTPAPGTGGRWGLGYAFGLLP
                     DGDRLAYHEGANRGWRAGLALLPDRRTGIVVLANSDAGSAPINAVVHQWVRLSTKPRR
                     RSPTGHDTSSGSLFRGPEGSMA"
     gene            complement(2126..2449)
                     /locus_tag="VFO47_12180"
     CDS             complement(2126..2449)
                     /locus_tag="VFO47_12180"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET8789494.1"
                     /translation="MSVVTEHLEQRGAAFEVLPHRQAYISIDEARALGVDADEVLKTL
                     AVRTRPGYVLVVIPAARRLDLQLVREALDDNHARLATELFGTGGITTVALAKRPERGR
                     DGPVA"
     gene            complement(2508..>3222)
                     /locus_tag="VFO47_12185"
     CDS             complement(2508..>3222)
                     /locus_tag="VFO47_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HET8789495.1"
                     /translation="IVGPDGAPWVTDGGLNAIVRVDPRTGKVDRYPLPPERGSANLNT
                     AAFRDGVLWFTGQAGVLGRLDPKVGRVEVFDAPRGPGPYGITTTPDGQVFYASLAGSH
                     IGRIDHRTGAAEVLEPPTGDQGARRVWSDSRGRIWVSEWNAGQLGRYDPADGRWQEWK
                     LPGANPMAYAVYVDDNDHPWLSDFAANALVRFDPSTERFTVVPLPSSPANVRQILGRP
                     GEVWGAESGVDKLVVVHTS"
BASE COUNT          453 a         1170 c         1145 g          454 t
ORIGIN      
        1 ggccgtcgtc gcctgatcga gcgcctggat ctcgtccccg gccagcgaga aggagagcgc
       61 ttcggcgttg ctggcggcct gtctcccgtt cttcgcaccc gggatcggga ggacaccctc
      121 ccgttcgatg agccaccgaa gggccacctg ggctggggtc ctggcatggc gttcaccgat
      181 ctcacgcagc agggccacga ccggctgcgc cttcttgaga ccatcgcccc ggaagtagcg
      241 gccgaagcgc cgaatcccct tgggccgatc gcccggccga tacttgccgg tgaggactcc
      301 ctgcgccatt ggctggtatg cgatcagcgt gatccccagc tcctggcagg cgtccagcag
      361 cccattcacc tccggcgccc ggtgcagcag ggagtactcc acctggttgg aggcgagagg
      421 aattccccgc tcggccagcg ccgcatgggc gacgcggagt tgtgctgcgg aatagttgct
      481 caccccaact gccctgacct tccccgcttc gaccgcgtcg gccatcaacc ccatgaggga
      541 ggggatcgag atgcgacgag aagggaagtg gtgctgatag aggtcgatgg ggcttcgccg
      601 gagccgagca aggctctggt ccagcgcctc cgggaatgcc tctgccgttg agagccagcc
      661 gggcgggaac ttggtggcga tcaccacgct ctggccctcg gcgagctcgc ccacgcgccg
      721 ctccgaagcg ccgccgctgt acatcgcggc cgtgtcgaac aggtccacgc ccgccgtcaa
      781 gctggcctcg aaggcccgcc gttcttcctc cggccctggg gggcctccat aggccagctt
      841 ggccggtccc cacctgctcc gacccgaggg gctgccccag gtcatcgccc ccaggcccag
      901 tcttggcacc tcgagatcag tacggccgag acggatccgg tcggtcatcc gcgcaccatc
      961 actcgccatg gcatgtcctt aagccatcga gccttccggt ccacggaaga gactaccgct
     1021 ggaggtgtcg tggccggtcg gggagcgccg ccgcggcttg gtgctgagcc tcacccattg
     1081 gtggaccacg gcgttgatgg gggcgctgcc ggcgtcgctg ttggccagca ccacgatgcc
     1141 cgtccgccgg tcgggcagca gcgccaggcc ggcccgccag ccgcggttgg cgccctcgtg
     1201 gtaggcgagc cggtcaccgt cgggcagcag gccaaatgcg tacccgagtc cccaccggcc
     1261 cccggtgccg ggggcggggg tgagggccag gcggaccccg gccgggcgga gcacccctcg
     1321 tcctggtggc tcgccctcgg ggccgggcag cgcggcggcg gcgaagcggg ccaggtccgg
     1381 cgcggtggtg accagcccgg cggcggcctg ttcggcgaag gcaaagtcgg ggacctggca
     1441 cccgtcgaca tcgtgcgggc gggcggtcgc cgccgtgcgg tgccactgga agctgctggc
     1501 agtcatgccg agcggcccca gtacctcggt ctgcatgacg tcggcgaatg gccggccggt
     1561 gagttcctcg accagcagct gcagcacgct gtagccgccg cccgagtaca gccagcgtcg
     1621 acccggtgcg tccagcagct ccaccgggaa gctcccgccg ccttcgcccg ccagcgaggc
     1681 atcaatggct ggcagcggcc gatccagggt ctggccgaca tagccgtgga ccgacaggcc
     1741 ggcggtgtgg ctgagcagcc gacggacggt gatgccgtcg gtcttgaatg gcgacggcgg
     1801 ggggcaccag cgtcgcaggc ggccgaccac cggctcgtcc aggtcgatcc ggccctgctc
     1861 gaccaggcgc agcacccccc aggcggtcac cggcttggag accgaggctg cctggaagcg
     1921 ggttgcgggc gtcaccggtt cgcctgtcgc ctggtcggcg accccgtagc cggccgccaa
     1981 cgcgatctgt ccgtgcctga cgagcgcgac ggccagcccg ggagtcatcg agtcagccag
     2041 tgcccgcact gccgcgtccc ccacggctgg cgccaagccg gagtccagcg ggtcgtgaga
     2101 tcgccgacgc accggcccac cctgcctacg cgaccggtcc gtcgcggcct cgttccggcc
     2161 gcttggccaa cgccacggtc gtgatcccgc cggtcccgaa cagctcggtg gccaggcggg
     2221 catggttgtc gtccaacgcc tcgcggacga gctgcagatc cagccggcgc gcggccggga
     2281 tgaccaccag cacatagccg ggccgggtgc gcaccgccag cgtcttgagc acctcatcgg
     2341 catcgacacc gagcgcgcgt gcctcgtcga tggagatata ggcctggcgg tgagggagca
     2401 cctcgaacgc ggcaccgcgc tgctcgagat gctcggtgac gaccgacatc acaaccacct
     2461 ccccattcgt ccagccccgc ccccggccgc ggacggacgc cgccggtcta gctggtatgg
     2521 accacgacga gcttgtccac gccggactcg gcgccccaca cctcgccggg ccggccgagg
     2581 atctggcgca cgttggccgg cgagctcggc agggggacga ccgtgaaccg ctcggtggag
     2641 gggtcgaacc ggaccagggc gttggcggcg aagtcgctca gccacggatg gtcgttgtcg
     2701 tccacataca cggcataggc catcgggttg gcgccgggca gcttccactc ctgccagcgg
     2761 ccgtcggccg ggtcgtagcg gcccagctgg ccggcgttcc actcgctcac ccagatccgg
     2821 ccgcgcgagt cggaccagac ccgacgggcg ccctggtcgc cggtgggcgg ctccagcacc
     2881 tcggcggcgc ccgtccggtg gtcgatgcgg ccgatgtggc tgccggccag ggaggcgtag
     2941 aacacctgcc cgtcgggggt ggtggtgatg ccgtaggggc caggccccct gggggcgtcg
     3001 aagacctcga cccgtccgac cttcgggtcg agccggccca gcacccccgc ctgcccggtg
     3061 aaccacagga caccatcgcg gaaggcggca gtgttgaggt tggccgagcc ccgctcgggc
     3121 ggcagcgggt agcggtcgac cttcccggtc cgcgggtcga cccgcacgat ggcgttcagg
     3181 ccgccgtcgg tgacccacgg ggccccgtcg gggccgacga tc
//
LOCUS       DASSWR010000726         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_706010,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000726 DASSWR010000000
VERSION     DASSWR010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706010"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..797
                     /locus_tag="VFO47_12190"
     CDS             207..797
                     /locus_tag="VFO47_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET8789496.1"
                     /translation="MLASLTGLGRSAAAGLHANIPLLLVGVLARFTDVLTLPEPYRWI
                     ESGWAIAIVSVLLVAELVLDKVPVVDHVNDVVQTLVRPTVGGVIFAATTAAEQADNST
                     WMQQHPWVGILLGVAVAGIVHATKATTRPLVNATTVGTGTPVVSAAEDAASLGMSLIA
                     VFIPILVVVVLLLLAWAAFAMYRRVRRRRRPAVTDG"
     gene            complement(878..1363)
                     /locus_tag="VFO47_12195"
     CDS             complement(878..1363)
                     /locus_tag="VFO47_12195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET8789497.1"
                     /translation="MPGPDGDGPMTRDDVLPAGARHCFACGDANPIGLHLNDIRREGD
                     RVLATLHPRPEFQSYPGVLHGGLSATALDEVMGYASILLAGIWAATATMDVRYRAQVP
                     YDAPLPLVAGLTDTRGRRVRAWSRLLLPSGEVGAEATALLVALPEDLASRARALYGPM
                     A"
     gene            1432..2475
                     /gene="add"
                     /locus_tag="VFO47_12200"
     CDS             1432..2475
                     /gene="add"
                     /locus_tag="VFO47_12200"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET8789498.1"
                     /translation="MAAVTDDALRRLPKAELHIHLDGSLRPATVEELAREVGLVAADA
                     PAGAAMARVRVTRRAGLLEALAGFEVLLPLLQTKEHLARVTTELVEDLAADGVVYAEL
                     RFCPRLNTEQGLTVDEVLATVGEAAAQADAASGTRVVLLACMMGGVDPEWNRPVLDAA
                     IRRIDSGVVGIDLAGPVEGRTPAWVDAHAAMFEHARASGLCVTVHAGEAEPPAAIRTA
                     LDRYLADRIGHATSLAEDPDLLAEVVERRVVLECCPRSNYWTRTEAELPSLSDHPVGG
                     LLRAGARVCLNTDDRTLFGNDLSSEYRVVAEAQGLDDRQVAALTANAFAGAFHPGRAS
                     AALERAVGRLDGA"
     gene            2479..>2647
                     /locus_tag="VFO47_12205"
     CDS             2479..>2647
                     /locus_tag="VFO47_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET8789499.1"
                     /translation="MTTTEAFTPADPAFVADPYPAYEGLRAAGRVLYDPASDHWLVPH
                     HADVNALLRDRR"
BASE COUNT          319 a          937 c         1035 g          356 t
ORIGIN      
        1 acctggccgc ccggatcgcc gcccacgccg gcgccgggca ggtgctggtc agccagggcg
       61 tcgccgagtc ggccgccccc gagggcgtgc gcttccagag gctgggcgag ctgcggctgc
      121 aggggttcgc ccgcccggtg cccctgctgg aggccagccg agcctgacgt tgtgtgacgg
      181 acccgtccag ggggaagcag gaggtcatgc tggcatcact caccggcctc ggccggtcgg
      241 cggcggcagg cctccacgcc aacatccccc tgctgctggt gggcgtcctg gcccgcttca
      301 ccgacgtcct caccctcccc gagccgtacc gctggatcga gtccggctgg gccatcgcca
      361 tcgtctcggt cctgttggtg gccgagctcg tgctcgacaa ggtgcccgtc gtcgaccacg
      421 tcaacgacgt cgtccagacc ctggtccgcc ccaccgtcgg cggcgtcatc ttcgccgcca
      481 ccaccgccgc cgagcaggcc gacaactcca cctggatgca gcagcacccc tgggtcggca
      541 tcctcctcgg cgtcgccgtc gccggcatcg tccacgccac caaggccacc acccgccccc
      601 tggtcaacgc caccaccgtt ggcaccggga ccccggtggt cagcgccgcc gaggacgccg
      661 cctccctcgg catgagcctg atcgccgtgt tcatcccgat cctggtcgtg gtcgtcctgc
      721 tcctgctcgc ctgggcggcg ttcgccatgt accggagggt ccggcgaagg cggagaccgg
      781 cggtgaccga cggctgagac ccagggtcag ggtcgcgagc tgctggaccg tggtgcctcc
      841 gggtcagcga ggaggctccg ggttggtggg gcgcttgtca ggccatcgga ccgtagaggg
      901 cgcgggcgcg ggaggccagg tcctcgggga gggcgaccag cagcgcggtc gcctcggcac
      961 cgacctcgcc ggatggcagc agcaggcggg accaggcgcg gacccgcctc cctctggtgt
     1021 cggtgaggcc ggcgaccagg gggagggggg cgtcgtaggg gacctgggcg cggtagcgga
     1081 cgtccatggt ggcggtggcg gcccagatgc cggccagcag tatcgacgca taccccatga
     1141 cctcgtccag cgctgtcgcc gacaggccgc cgtggaggac gccggggtag ctctggaact
     1201 cggggcgggg gtggagggtg gccaggaccc ggtcgccttc gcggcggatg tcgttcaggt
     1261 gcaggccgat ggggttggcg tcgccgcagg cgaagcagtg gcgggctccg gccgggagca
     1321 cgtcgtcgcg ggtcatggga ccgtcgccgt cgggaccggg catgggcgct cctcgatggt
     1381 gggtgggcag gtaggctacg cctgccgcga acgtgcgctc aggaggtctg gttggctgcc
     1441 gtcaccgatg acgctctgcg ccggctgccc aaggccgagc tgcacatcca tctcgacggg
     1501 tcgttgcggc cggcgacggt cgaggagctg gcccgggagg tggggctggt ggcggcggac
     1561 gcgcccgcgg gggcggcgat ggccagggtg cgggtgaccc ggcgggcggg gctgctggag
     1621 gcgctcgccg ggttcgaggt gctgctgccg ttgctgcaga ccaaggagca tctggccagg
     1681 gtgaccaccg agctggtgga ggacctggcc gcggacgggg tggtctacgc cgagctgcgg
     1741 ttctgcccgc ggctcaacac cgagcagggg ctgaccgtcg acgaggtgct ggccacggtc
     1801 ggggaggcgg ccgcccaggc cgacgcggcc agcgggaccc gggtggtgct gctggcctgc
     1861 atgatgggcg gggtcgaccc ggagtggaac cggccggtgc tggacgcggc catccggcgg
     1921 atcgactcgg gggtggtcgg gatcgacctg gccgggccgg tcgagggccg gaccccggcg
     1981 tgggtcgacg cccacgcggc catgttcgag cacgcccggg cgtcggggct gtgcgtcacc
     2041 gtccacgccg gcgaggccga gccgccggcg gccatccgga ccgcgctgga ccgctacctg
     2101 gccgaccgga tcgggcacgc caccagcctg gccgaggacc ccgacctgct cgccgaggtg
     2161 gtcgagcgcc gggtggtgct ggagtgctgc ccgcggtcca actactggac ccggaccgag
     2221 gccgagctgc ccagcctgtc cgaccacccg gtgggggggc tgctgcgcgc cggggcccgg
     2281 gtgtgcctca acaccgacga ccggaccctg ttcggcaacg acctgtcctc ggagtaccgg
     2341 gtggtggccg aggcccaggg gctggacgac cggcaggtgg cggccctgac cgcgaacgcg
     2401 ttcgcggggg cgttccaccc gggccgggcc tcggccgccc tggagcgggc cgtcggccgg
     2461 ctcgacgggg cttgaccgtt gaccacgacc gaggcgttca ccccggccga cccggcgttc
     2521 gtggccgacc cctacccggc gtacgagggg ctccgggcgg ccgggcgggt cctgtacgac
     2581 cccgccagcg accactggct cgtcccccac cacgccgacg tcaacgcgct gctccgcgac
     2641 cggcggt
//
LOCUS       DASSWR010000727         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_25757,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000727 DASSWR010000000
VERSION     DASSWR010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25757"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFO47_12210"
     CDS             <1..558
                     /locus_tag="VFO47_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET8789500.1"
                     /translation="MLRALDEFRIEGVPTTIPFHRLAMTDPAFVAGDVSTVLVEQGMD
                     LSSLEPAVLDGAGPPTAKPPPRRMVIELEGTRFDVDLFPQEPVKVPERIRTPRSPGAL
                     ERARAETGGPGKEVVKTPMQGTIVKVLVAEGDTVKAGQTLVVLEAMKMENHVTAHQAG
                     TVAGLEVSEGQTVPTGATIAVIEAT"
     gene            565..978
                     /locus_tag="VFO47_12215"
     CDS             565..978
                     /locus_tag="VFO47_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789501.1"
                     /translation="MTGAPSVAEWARANPAGRDALLALAERAVALQPAAEAAIQHCRS
                     GPEQAAQAQDAAAARDAYHSLAGHLRALGLEAPVAAEVAGLLDGHLEALDQALGGRGV
                     EGLGPVAGRLVGARDLLRRTVAIGGRPVTDVTPSV"
     gene            1012..>2408
                     /locus_tag="VFO47_12220"
     CDS             1012..>2408
                     /locus_tag="VFO47_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET8789502.1"
                     /translation="MQLARSLLMLPAGRVAKWVVLAAWVAVAALVAPFAGQLQSVQEN
                     EAASFLPSSAESTRALELQRQLPGGDRLPVVVVYRRESGITPADRQLAAGHQQALAQG
                     QEGPPPVPSGDGRALLLVLSLPAADADAVIAEIEGIRETVGRGDGDLQIRVTGPGGFL
                     ADTVSVFEDIDTTLLLVTVVVVAVLLLLTYRSPLLWLIPLLTVGLANQAATASVYGLV
                     KGFGLTVDGQSAGILTVLVFGAGTDYALLLIARYREELRRHDDKHEAMAVALANAGPA
                     ILASAATVIISLLCLLAADLANYRSLGPVGAVGVACALVAMLTLLPAVLVIFGRRLFW
                     PFVPSFGSEAREDAGVWARVGRWVAHRPRPVWLGTTAILAVLALGLLTLDTNLRQEDQ
                     FPGEPDAVAGQRLIEASYPSGTGQQTTIIGAAGPAEQVLAAARGTEGVAAVVPAGRTA
                     DLVQLQATLDAAPDS"
BASE COUNT          281 a          948 c          869 g          310 t
ORIGIN      
        1 atgctccggg cccttgacga gttccggatc gagggcgtcc ccaccaccat cccgttccac
       61 cgcctggcca tgaccgaccc ggcgttcgtg gccggggacg tctccaccgt cctggtcgag
      121 cagggcatgg acctgtcgtc gctggagccg gcggtgctgg acggggccgg gccacccacg
      181 gccaagccgc cgccccggcg gatggtcatc gagctggagg gcacgcgctt cgacgtcgac
      241 ctgttccccc aggagccggt caaggtgccg gagcggatcc gcaccccccg ctcccccggg
      301 gccctggagc gggcccgggc ggagacgggc gggccgggca aggaggtcgt caagaccccc
      361 atgcagggca cgatcgtcaa ggtgctggtg gccgagggcg acaccgtcaa ggccgggcag
      421 accctggtcg tcctcgaggc catgaagatg gagaaccacg tcaccgccca ccaggcgggc
      481 accgtggccg gcctggaggt cagcgagggc cagacggtcc cgaccggcgc caccatcgcc
      541 gtcatcgagg cgacctaggc cgccgtgacc ggcgccccgt cggtggccga gtgggcccgg
      601 gccaacccgg ccggccgcga cgccctgctc gccctggccg agcgggcggt cgccctccag
      661 ccggccgccg aggccgccat ccagcactgc cgttccggcc ccgagcaggc cgcccaggcc
      721 caggacgccg ccgccgcccg cgacgcctac cactcgctcg ccggtcacct gcgcgccctc
      781 ggcctggagg cgccggtcgc ggccgaggtc gccgggctgc tcgacgggca cctggaggcg
      841 ctcgaccagg ccctgggcgg ccgtggcgtc gagggcctgg ggccggtggc cgggcggctg
      901 gtcggggccc gcgacctgct gcggcggacg gtggcgatcg gggggcggcc cgtgaccgac
      961 gtcaccccct ccgtttgaca gccccggatt ctctggtagg actggccacc gatgcagctc
     1021 gcccgttccc ttctcatgct gcccgccggc cgggtggcca agtgggtcgt gctcgccgcc
     1081 tgggtggcgg tggcggccct ggtcgccccg ttcgccggcc agctccagtc ggtgcaggag
     1141 aacgaggcgg ccagcttcct gcccagcagt gccgagtcga ccagagccct ggagctgcaa
     1201 cgccagctcc ccggcggcga ccgcctgccg gtggtggtgg tctaccggcg cgagtcgggc
     1261 atcaccccgg ccgaccgcca gctggccgcc ggccaccagc aagcgctcgc ccagggccag
     1321 gaggggccgc cgccggtgcc gtccggggac ggcagggcgc tgctgctggt gctctccctg
     1381 cccgccgccg acgccgacgc cgtcatcgca gagatcgagg gcatccgtga gacggtcggc
     1441 cggggcgatg gcgacctcca gatccgcgtg accgggccgg gcgggttcct ggccgacacc
     1501 gtcagcgtgt tcgaggacat cgacaccacc ctgctgctgg tcaccgtggt cgtggtcgcg
     1561 gtgctgctgc tgctcaccta ccgcagcccc ctgctctggc tgatccccct gctgaccgtc
     1621 ggcctggcca accaggcggc cacggccagc gtgtacgggc tggtcaaagg cttcggcctg
     1681 accgtcgacg gccagagcgc gggcatcctg accgtgctcg tgttcggggc cgggaccgac
     1741 tacgcgctgc tgctgatcgc ccgctaccgg gaggagctgc gccgccacga cgacaagcac
     1801 gaggccatgg ccgtggccct ggccaacgcc ggcccggcca tcctggcctc ggcggccacg
     1861 gtcatcatca gcctcctctg cctgctcgcg gccgacctgg ccaactaccg cagcctcggc
     1921 cccgtcgggg ccgtcggcgt cgcctgcgcc ctggtggcca tgctgaccct gctcccggcc
     1981 gtgctggtga tcttcgggcg gcggctgttc tggccgttcg tcccctcctt cggctccgag
     2041 gcccgcgagg acgccggcgt gtgggcgcgg gtcggccgct gggtcgccca ccggccccgg
     2101 cccgtctggc tgggcaccac cgcgatcctg gccgtgctcg ccctcggcct gctcaccctc
     2161 gacaccaacc tgcgccagga ggaccagttc cccggcgagc ccgacgcggt cgccggccag
     2221 cggctgatcg aggccagcta cccgtccggc accggccagc agaccaccat catcggcgcc
     2281 gccggcccgg ccgagcaggt cctggccgcg gcccgcggca ccgagggcgt ggccgcggtg
     2341 gtcccggccg ggcggacggc cgacctggtc cagctccagg cgaccctgga cgccgccccc
     2401 gactccga
//
LOCUS       DASSWR010000728         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_819289,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000728 DASSWR010000000
VERSION     DASSWR010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819289"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFO47_12225"
     CDS             <1..273
                     /locus_tag="VFO47_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789503.1"
                     /translation="RPGAGNHCRPVAGQDSRPGEAWLLSPELVSISYAATELDLFEPV
                     ARQVGPAAYKVGPRNSEWVATLYLAQPVRQGAGGIWVITRTGSPAG"
     gene            536..739
                     /locus_tag="VFO47_12230"
     CDS             536..739
                     /locus_tag="VFO47_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET8789504.1"
                     /translation="MTVGTVKWFNANRGYGFIQPESGNDVFVHASAIQDGDSLQDGQA
                     VEFDITQGQKGPQAANVRPVAAQ"
     gene            825..1178
                     /locus_tag="VFO47_12235"
     CDS             825..1178
                     /locus_tag="VFO47_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8789505.1"
                     /translation="MAPLLQSATTPSEGDAVVTVRGDIDAATAPQLWQYLSYLVGQGH
                     HHIVLDLRGMVLIDSAGVEILGRVSAWTRRKGGELTLRSVSPALAEQLELARRAQAHR
                     HHPSTAGDQPPSPTR"
     gene            1175..1483
                     /locus_tag="VFO47_12240"
     CDS             1175..1483
                     /locus_tag="VFO47_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789506.1"
                     /translation="MTPEGTMPDDRGMSPGLLQQLTLAILDHSAEEGLRMIATVRSYL
                     DTWEVFLQDRHELVDHLRAAHHADPACTQADPASLNRQHWHAHGSDLTSFLDAHPVQG
                     "
     gene            1574..1843
                     /locus_tag="VFO47_12245"
     CDS             1574..1843
                     /locus_tag="VFO47_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789507.1"
                     /translation="MLPNQPVPLVDPEVERLAHVMVDGLIERLAAEPAVPATSLLDQL
                     AEQLQQAASLRRLNVMRRIPGSTSAAWSPATPRRTARSPASEGQP"
     gene            2111..3316
                     /locus_tag="VFO47_12250"
     CDS             2111..3316
                     /locus_tag="VFO47_12250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8789508.1"
                     /translation="MAPRTAFAELGVPSILTAALAARGITTPRPIQARTLPDALAGRD
                     VLGRAETGSGKTLAFGLPLLVRTATASGKRRARQPRGLVLVPTRELADQVHDELLPLA
                     AVLGLRLLTVYGGVPLGRQAQELRRGIDVVVATPGRLLDLLGQDACSLDAVATAVLDE
                     ADHMADMGFLPAVTSLLDRVPPGGQRLLFSATLDGQVDTLVRRYLTDPVRHTVAPTAA
                     ADRMDHQIRTVKATDKVAVAAELAGRPGRTLVFVRTKHRAARLARQLDRAGIRAGALH
                     GNLTQPARTRALAAFAAGTVPVLVATDIAARGIHVDGIDLVIHFDPPAEPKAYLHRSG
                     RTARAGAAGTVITLVLPEQATEVARLHRRAGVATPPLPVDPTTSRPARTGARRPGGRR
                     RRRRVTASA"
BASE COUNT          484 a         1362 c         1149 g          495 t
ORIGIN      
        1 cgtcccggcg ccgggaacca ttgtcggcct gtggccggtc aggactctcg cccaggcgag
       61 gcctggctgc tgtcgcccga actcgtctcg atctcgtatg cggccaccga gctcgacctg
      121 ttcgagccgg tggcgcgcca ggtgggcccg gccgcctaca aggtgggtcc ccgcaactcc
      181 gagtgggtgg ccaccctcta cctggcccag cccgtccgcc agggcgctgg cggcatctgg
      241 gtcatcaccc ggaccgggag cccagcgggc tagccggcag tggcctggtc tcgcgaccat
      301 cgcacggtgg ccgcttccac ctgagcagcc acgatggtgc tggggtgggt ccaggtcgtc
      361 tgcgtcgtgg actggcatga cgagcacccg gtcggcgggg cggcgcggcc ggtgtcgtgc
      421 taccctcgag ttagcacggg ttcacggttg acttgtagtt cacggtcgag cttgtccctt
      481 tgccgccggc gtcggcgtga tcgatacctc cgagctgatg gagggaggga caaccatgac
      541 tgttggaacc gtcaagtggt tcaacgccaa cagaggctac ggcttcatcc agcccgagtc
      601 cggcaacgac gtcttcgtgc acgccagcgc catccaggac ggcgactcgc tgcaggatgg
      661 ccaggccgtc gagttcgaca tcacccaggg ccagaaaggc ccccaggccg ccaacgtgcg
      721 accggtcgcg gcccagtagc cgctggggag cgattctggc tgcggttcgc gggagcggcg
      781 gacctgctgg gtgccagcgc gccgctcacg gtaaggaggc cgcgatggcc ccgctgttgc
      841 agtccgccac caccccgtcc gagggcgacg ccgttgtcac cgtgcgcggc gacatcgacg
      901 ccgccactgc gccccagctc tggcagtacc tgagctacct ggtcggtcag ggccaccacc
      961 acatcgtgct ggacctgcgg gggatggtcc tgatcgactc ggccggggtc gagatcctgg
     1021 gccgggtgtc ggcctggacc cgccgcaagg gtggcgagct gaccctgcgg tcggtgagcc
     1081 cggccctggc cgagcagctg gagctggccc gccgggccca agcccaccgg catcatccct
     1141 cgaccgccgg cgaccagccg ccatccccga cgcgatgacc ccagagggaa ccatgcccga
     1201 cgaccggggc atgtccccag ggctgttgca gcagctgacg ttggccatcc ttgaccactc
     1261 ggccgaggaa ggccttcgca tgatcgcgac ggtccgctcc tatctggaca cctgggaggt
     1321 cttcctgcag gaccggcacg agctggttga ccatctgcgc gccgcccacc acgccgaccc
     1381 cgcctgcacc caggccgatc cggccagcct caaccgccag cactggcacg cccacggcag
     1441 cgacctgacc agcttcctgg acgcccaccc agtccaggga tgacgacctc ggtcatccga
     1501 cgcccggccg aagcaggcgc gaggagtcaa aggagttgat ccgagcatgt cccgtcttgg
     1561 gggtacaccc cggatgttgc ccaaccagcc ggtcccgctt gtcgatcctg aggtcgaacg
     1621 gctcgcccat gtgatggtgg acgggttgat cgaacggttg gcggccgagc cggcggtccc
     1681 tgccaccagt ctgctcgacc aactggccga gcagctccag caggcagcga gcctgcgccg
     1741 gctcaacgtc atgcgccgca tccctgggtc cacgtcggcc gcctggtcgc ccgcgacccc
     1801 gcggcggaca gcgcggtcgc ctgcctcgga gggacagccc tgacaccagc cctgaactga
     1861 cccggcgagg cacgagcacc ccgcgggtac ccggttccgt ccaggtggtc tgctatcttc
     1921 gccacatacg ctttcgagcg agggcgatct gtcctcgcgg tcgtcacaag ccacgtactt
     1981 cgttcgtacg cgcgccagct cccggcgcag gtcgtccgcg cgccgtccgg cacttggggc
     2041 gcccgaccca ccccgaacgg agtgtcgttc ccacttgcct gcaaccccta ccacctcgat
     2101 cccgacccgc atcgcacccc gaactgcctt tgccgagctc ggcgtcccct cgatcctgac
     2161 tgcggcgctg gctgcccggg gcatcaccac cccccgcccg atccaggccc gcaccctgcc
     2221 cgacgccctg gccggccggg acgtcctcgg ccgggctgag accggctcgg gcaagaccct
     2281 tgccttcggg cttccgctgc tggtccggac cgccaccgca agcggcaagc ggcgggcgcg
     2341 gcagcctcgc ggcctggtgc tggtcccgac ccgggagctg gccgaccagg tccacgacga
     2401 gctcctcccg ctggccgccg tgctcggcct gcggctgctg accgtctacg gcggcgtgcc
     2461 gctcggccgg caggcccagg agctccggcg tggtatcgac gtcgtcgtcg ccacccccgg
     2521 gcggctgctg gacctgctgg gccaggacgc ctgctcgctg gacgcggtcg ccaccgccgt
     2581 ccttgacgag gccgaccaca tggccgacat gggcttcctg cccgcggtca cctcgctgct
     2641 ggaccgcgtc ccgcccggag gtcagcggct gctgttctca gccaccctcg acggccaggt
     2701 cgataccctc gtccgccgct acctgaccga cccggtgcgg cacacggtcg cgcccaccgc
     2761 ggctgccgac cggatggacc accagatccg caccgtgaag gcaaccgaca aggtcgcggt
     2821 cgcggccgag ctcgcgggcc gccctggccg gacgctggtg ttcgtccgca ccaagcaccg
     2881 cgccgcccgc ctggcacgcc agctggaccg cgccggcatc cgggctggcg ccctgcacgg
     2941 caacctgacc caaccggcca gaacccgtgc ccttgccgcc ttcgccgccg gcacggtccc
     3001 ggtgctggtg gccaccgaca tcgccgcccg cggcatccac gtggacggga tcgacctcgt
     3061 catccacttc gacccaccgg ccgagcccaa ggcctacctg caccgctcgg ggcgcaccgc
     3121 acgcgccggc gctgccggca ccgtgatcac cctggtcctg cccgaacagg ccaccgaggt
     3181 cgccaggctg caccggcgcg ccggtgtcgc cactccaccc ctgccggtcg atccgaccac
     3241 ctcacggccg gcccggaccg gcgcgcgccg ccccggcggc cgccgtcgcc gtcgccgtgt
     3301 gaccgcatcc gcctaggacc tgcacggcag ggtcacagcc gcgcgtgcgc cgccgtcgga
     3361 cacgctgacg gacgatgagc ctcccgaccg ggccccagtt tgggctcgcg ctggcgggct
     3421 agccacttgg cagcagccga cccgcaaccg gtcagctcgg tccctgcggc ccgaggcccc
     3481 ggaaggagac
//
LOCUS       DASSWR010000729         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1186722, whole genome shotgun sequence.
ACCESSION   DASSWR010000729 DASSWR010000000
VERSION     DASSWR010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186722"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFO47_12255"
     CDS             complement(<1..574)
                     /locus_tag="VFO47_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET8789509.1"
                     /translation="MSEPFGLAEVEGAAARIAGRVHRTPVVTSRLLDRELGCRLAMKG
                     EHLQRVGAFKARGAFSALLALDPATRANGVLAVSSGNHGQAVALAAAETGGRAVVVMP
                     AGSNPAKVAATRAYGAEVVTAGVTGENRETFAADLAAERGLRLVHPFDDHDVMAGQGT
                     AALELLDDVGDLDLVLVPVGGGGLISGTAAA"
     gene            complement(571..1599)
                     /locus_tag="VFO47_12260"
     CDS             complement(571..1599)
                     /locus_tag="VFO47_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET8789510.1"
                     /translation="MTTVDLRSDTFTRPTPAMRRAMADAEVGDDVWGEDPTIDRLEAA
                     VAERLGHEAALYVPSGTMGNQICLRLAAPPGTEVLANEDNHILWYESAAAPVLSGVQL
                     HPVPGPRGMLEVAAARARVRTERYGVPTSAIAVENTHNRAGGAVQPLEVLEGLRALAD
                     EAGLHLHCDGARIWNASVASGVPLERYGALFDSLSVCFSKGLGAPVGSAVVGPSDLVG
                     RAREVRHLYGGAMRQAGVLAAAALVALEQNLDRLADDHANARALAETVAAAVPGSVDP
                     GAVDTNMVLLDSGPFGTDGPGLVERLEELGVLAADTDPDRVRLVFSYEVSASDGERAA
                     KAVVAAAG"
     gene            1672..2901
                     /locus_tag="VFO47_12265"
     CDS             1672..2901
                     /locus_tag="VFO47_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HET8789511.1"
                     /translation="MASTPLPHNDTLEIRGHIIDSMLFTRILDQVLEAHGSYEIEQFD
                     VGRTPVDPSYARLRIYTDDPERLEELVQSLMGLGMQRLETGDATLVPAEMDGVLPDGF
                     YSSTNLETEVRVAGAFVRVENPEMDCAIVVRDRRARTVPMSDVLKGEQVVVGSQGIRV
                     QPLDRGRSGEVFEFMSSQVSSEKPQGLIVRRVAGAMRATKERGERILWVGGPAVVHTG
                     AGPSVVRLIEAGYMDVLFAGNALATHDIEGALYGTSLGVNLAEGIGVEHGHEHHIRAL
                     NTIRRCGSVAKAVEQGVLTSGIMHACVVNGVDFVLGGSVRDDGPLPDTVTDVVEAQRQ
                     MRSLLGGVGYALMVASMLHSIATGNILPASVPLVCVDINPATVTKLADRGSAQATGVV
                     TDVGLFLGALADELAPR"
     gene            complement(2846..>3169)
                     /locus_tag="VFO47_12270"
     CDS             complement(2846..>3169)
                     /locus_tag="VFO47_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET8789512.1"
                     /translation="IDWLLWFAAMSPPSAHPWMATLVTRLLERDPATLALLGRPDPFP
                     DHPPALVRARLYRYRFTTPAERRQTGAWWSRDLVGEYLPPLRLDPLSGAPARRPGRPG
                     TARRR"
BASE COUNT          407 a         1174 c         1191 g          397 t
ORIGIN      
        1 ccgcggccgc ggtgccggag atcagcccgc ccccgcccac cgggaccagc accaggtcca
       61 ggtcaccgac gtcgtcgagc agctccaggg cggccgtgcc ctgcccggcc atgacgtcgt
      121 ggtcgtcgaa gggatggacc aggcgcaggc cgcgctcggc ggccaggtcg gcggcgaagg
      181 tctcgcggtt ctcgccggtc acgcccgcgg tgaccacctc ggcgccgtag gcccgggtgg
      241 cggccacctt ggccgggttg gacccggccg gcatcaccac caccgcccgg cccccggtct
      301 cggccgcggc cagggcgacc gcctggccgt ggttgccgga gctgaccgcc aggaccccgt
      361 tcgccctggt cgccgggtcg agggcgagca gggcgctgaa cgcccccctg gccttgaacg
      421 cccccacccg ctgcaggtgc tcgcccttca tggccagccg gcagccgagc tcgcggtcca
      481 gcaggcggga ggtcacgacc ggggtgcggt ggacccgccc ggcgatccgg gccgccgccc
      541 cctcgacctc ggcgagcccg aacggctcgc tcatccggcg gcggcgacga ccgccttggc
      601 ggcccgctcg ccgtcgctgg cgctgacctc gtagctgaag accagccgca cgcggtcggg
      661 gtcggtgtcg gcggccagca cccccagctc ctccagccgc tccaccaggc cggggccgtc
      721 ggtgccgaag gggccgctgt ccagcaggac catgttggtg tcgacggcgc cggggtcgac
      781 gctgccgggg acggccgcgg ccaccgtctc ggccagggcg cgggcgttgg cgtggtcgtc
      841 ggccaggcgg tcgagattct gctcgagggc gaccagggcg gccgcggcca gcaccccggc
      901 ctggcgcatg gccccgccgt acaggtggcg aacctcgcgg gcccgtccga ccaggtcgct
      961 ggggccgacc acggccgacc cgacgggcgc gccaaggccc ttggagaagc agaccgacag
     1021 gctgtcgaac agggcgccgt agcgctccag cggcaccccg gaggcgaccg aggcgttcca
     1081 gatgcgggcc ccgtcgcagt gcaggtgcag cccggcctcg tcggccaggg cccgcagccc
     1141 ctccaggacc tccaggggct ggacggcccc gccggcccgg ttgtgggtgt tctcgaccgc
     1201 gatggcgctg gtcgggaccc cgtaccgctc ggtccggacc cgggccctgg ccgcggccac
     1261 ctccagcatc ccgcgggggc cggggaccgg gtggagctgg acgccggaca gcaccggggc
     1321 ggccgcggac tcgtaccaga ggatgtggtt gtcctcgttg gccaggacct cggtcccggg
     1381 cggggcggcc aggcgcaggc agatctggtt gcccatggtg ccggacggga cgtacagggc
     1441 cgcctcgtgg ccgaggcgct cggccacggc cgcctccagg cgatcgatgg tcgggtcctc
     1501 gccccagacg tcgtcgccga cctcggcgtc ggccatggcc cggcgcatcg ccggggtggg
     1561 ccgggtgaag gtgtcggagc ggaggtcgac ggtggtcatc gcccgccagt ctacgcgcgg
     1621 gcgtgcgaca atcgtccgtc cgccacgacc cgcgacccgc accgaggagc catggcctcc
     1681 acgccgctgc cccacaacga caccctggag atccgcggcc acatcatcga ctccatgctg
     1741 ttcacccgca tccttgacca ggtgctggag gcgcacggca gctacgagat cgagcagttc
     1801 gacgtcggcc gcaccccggt cgacccctcc tacgcgcgcc tgaggatcta caccgacgac
     1861 cccgagcgcc tggaggagct ggtccagtcg ctcatgggcc tgggcatgca gcgcctggag
     1921 acgggcgacg ccaccctggt cccggccgag atggacgggg tcctgcccga cggcttctac
     1981 tcctcgacca acctcgagac cgaggtgcgg gtggccggcg ccttcgtccg ggtggagaac
     2041 ccggagatgg actgcgcgat cgtggtccgc gaccggcggg cccgcaccgt gcccatgtcc
     2101 gacgtgctca agggcgagca ggtggtggtg ggcagccagg gcatccgggt ccagccgctg
     2161 gaccggggcc gctccggcga ggtcttcgag ttcatgtcct cccaggtgtc gagcgagaag
     2221 ccccaggggc tgatcgtccg ccgggtcgcc ggggcgatgc gggccaccaa ggaacgcggc
     2281 gagcggatcc tgtgggtcgg cggcccggcg gtggtccaca ccggtgccgg cccgtcggtg
     2341 gtgcggctga tcgaggccgg ctacatggac gtgctgttcg ccggcaacgc cctggccacc
     2401 cacgacatcg agggcgccct gtacggcacc tcgctcgggg tcaacctggc cgaggggatc
     2461 ggggtcgagc acggccacga gcaccacatc cgggccctga acaccatccg ccgctgcggc
     2521 tcggtcgcca aggcggtcga gcagggcgtg ctcacctccg ggatcatgca cgcctgcgtg
     2581 gtcaacgggg tcgacttcgt cctcggcggc tcggtccgcg acgacggccc cctccccgac
     2641 accgtcaccg acgtggtcga ggcccagcgc cagatgcgtt cgctcctcgg tggggtcggc
     2701 tacgccctga tggtggcctc catgctccac tcgatcgcca ccggcaacat cctccccgcc
     2761 tcggtcccgc tggtctgcgt ggacatcaac ccggcgaccg tgaccaagct cgccgaccgc
     2821 ggctcggccc aggccaccgg cgtggtcacc gacgtcgggc tgttcctggg cgccctggcc
     2881 gacgagctgg cgccccgctg agcgggtcga ggcggagcgg cggcaggtac tccccgacca
     2941 ggtcgcggga ccaccaggcg ccggtctggc ggcgctcggc cggggtggtg aagcggtagc
     3001 ggtagaggcg ggcccggacc agggccggcg ggtggtcggg gaacgggtcg gggcggccga
     3061 gcagggccag ggtggccggg tcgcgctcga gcaggcgggt gaccagggtg gccatccagg
     3121 ggtgggccga gggcggcgac atggccgcga accacagcag ccagtcgat
//
LOCUS       DASSWR010000730         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1073575, whole genome shotgun sequence.
ACCESSION   DASSWR010000730 DASSWR010000000
VERSION     DASSWR010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073575"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>2091
                     /locus_tag="VFO47_12275"
     CDS             151..>2091
                     /locus_tag="VFO47_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET8789513.1"
                     /translation="MAQPVQTTTRRSGGAALLAIPLLAVLVLAGIGLAANLRAGARAE
                     RVRGLAPFTGALNTLVHDLQRERSLSAAQAPSRPALDAARAAVDGSAARYRDAAVQVE
                     ISDRDRQLHQRLDSGLARLAELTALRASVGAAGRGGESDPVTVFRRYTEIIGNLLAVG
                     GEIGLREAGQDAGLLQAVTAATAFSRAKELADREREAAAQAAASGALAPTERTRLAAL
                     GGRQDVLLDQFAALASPGQQARYARAFPAAEGGQTARLRRAALAAGPGPGAEADRAGW
                     SKATTTRFERMREVELGLTAEVAALAAQADRAAARRVLAYAMVLFLAASALVVLLVLT
                     PGRTRRSNAPDRVPPRDRAPGGVPPRDRALDPGTPEPHPPVPAPPAGERSLTATGEFA
                     ALHTPHPLPPASRGSHTPPPAVHTSPVGEVGPELADLARRSQDLVDQQLELLDQVARD
                     QADPRLPGRLLQVDRLAVRARRNAHNLIVLAGGEPGGAWEESAPLAEVVSVSVRDNPD
                     AHRVDLEVTDDLLVPGSATDDLANLLAELVDNATSFSAPETRVRVGGQKVGSGYVLEV
                     EDRGLGMTDGELVAVNRRLSGEQAAGDDLEQRLGTWVAGRLARRHNVRVQLRRSAYGG
                     VTALVFLPEPLVAAAEPAAPAGP"
BASE COUNT          240 a          850 c          761 g          240 t
ORIGIN      
        1 gttcctgcgc ctgcgctcga tcggcgccga cctcgccaag gcgggagacc gttcgtcaga
       61 tcagtgacgt acgcgatgct tgaccgatag ttgaggcgga accctccgaa cctgtttcat
      121 acccgctctg tgatgcggac cggagaggac atggcgcagc ccgtccagac caccacacgc
      181 cggtcggggg gagcggctct cctggcgatc ccgctgttgg ccgtgctggt ccttgccggc
      241 atcggcctgg ccgccaacct ccgggccggt gcccgggccg agcgggtgcg ggggctggcg
      301 cccttcaccg gcgccctgaa caccctggtc cacgacctcc agcgggagcg gagcctgtcc
      361 gccgcccagg cgccgtcccg gcccgccctg gacgcggccc gggcggcggt cgacgggtcc
      421 gccgcccgct accgggacgc cgccgtccag gtcgagatct cggaccgcga ccgccagctc
      481 caccagcgcc tcgacagcgg gctggcccgg ctggccgagc tcaccgccct gcgggcctcg
      541 gtcggcgcgg ccgggcgggg gggcgagtcc gacccggtca ccgtgttccg ccgctacacc
      601 gagatcatcg gcaacctgct ggccgtcggc ggcgagatcg gcctgcgcga ggccggccag
      661 gacgccgggc tgctccaggc cgtcaccgcc gccaccgcct tctcccgggc caaggagctc
      721 gccgaccgcg aacgcgaggc cgccgcccag gccgccgcca gcggcgccct cgcccccacc
      781 gagcggaccc gcctggccgc cctcggcggc cgccaggacg tcctgctcga ccagttcgcc
      841 gccctggcca gccccggcca gcaggccagg tacgcccgcg ccttccccgc cgccgagggc
      901 ggccagaccg cccgcctccg ccgcgccgcc ctggccgccg ggccgggccc aggggccgag
      961 gccgaccggg ccgggtggtc caaggccacc acgaccaggt tcgagcgcat gcgggaggtc
     1021 gagctcggcc tgaccgccga ggtggccgcc ctggccgccc aggccgaccg ggccgccgcc
     1081 cgccgggtcc tggcctacgc catggtcctg ttcctcgccg ccagcgccct ggtcgtcctc
     1141 ctggtcctga ccccgggccg cacccgccgt tccaacgccc cagatcgcgt cccaccccgc
     1201 gaccgggccc cgggtggcgt cccaccccgc gaccgggccc tcgacccggg cacgccggaa
     1261 ccccacccgc cggtgccggc ccccccggcg ggggagcgta gcctaacggc gacgggcgag
     1321 ttcgcggctc tccacacccc ccaccccctg cctccggcga gccgcggctc ccacaccccc
     1381 cctccagctg tccacacctc cccggtgggc gaggttgggc cggagctggc ggacctggcc
     1441 aggcggagtc aggacctggt ggaccagcag ctggagctgc tcgaccaggt cgcgcgggac
     1501 caggccgacc cgcggctgcc ggggcggctg ctccaggtcg accggctggc cgtgcgggcc
     1561 cggcgcaacg cccacaacct gatcgtgctg gccggggggg agcccggtgg cgcctgggag
     1621 gagtcggcgc cgctggccga ggtcgtctcg gtgtcggtcc gcgacaaccc ggacgcccac
     1681 cgggtcgacc tggaggtcac cgacgacctg ctggtgcccg ggtcggcgac cgacgacctg
     1741 gccaacctgc tggccgagct ggtggacaac gccacctcct tctcggcccc ggagaccagg
     1801 gtgcgggtcg gcgggcagaa ggtcggctcc ggctacgtcc tggaggtcga ggaccgcggc
     1861 ctgggcatga ccgacgggga gctggtcgcg gtcaaccggc ggctgtccgg cgagcaggcg
     1921 gccggcgacg acctggagca gcggctcggc acctgggtgg ccgggcgtct ggcccggcgt
     1981 cacaatgtcc gggtgcagct gcgccgctcg gcctacggcg gggtgacagc gctcgtgttc
     2041 ctgcccgagc cgctggtcgc ggcggccgag ccggcggcac cggccggacc g
//
LOCUS       DASSWR010000731         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1129352, whole genome shotgun sequence.
ACCESSION   DASSWR010000731 DASSWR010000000
VERSION     DASSWR010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129352"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..585
                     /locus_tag="VFO47_12280"
     CDS             124..585
                     /locus_tag="VFO47_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8789514.1"
                     /translation="MSPETKPTTRVCSITDALSIVGDRYALLVARELRYGLTRFQDIA
                     ASTGAPRDVLTSRLRKLEQAGVIERRPYCEHPPRYEYLLTDAGRDLQPILLALKEWGD
                     RHCNPGAEPIVFQHTCGADFHPLTVCAACRGPLRDGDLTVTGGTHPVQATM"
     gene            711..1367
                     /locus_tag="VFO47_12285"
     CDS             711..1367
                     /locus_tag="VFO47_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8789515.1"
                     /translation="MATAGGRRPPASRRAGGRRSQPYDADSLLEVAVKVFTERGYDGT
                     SMEDLARAARITKSSFYYHVSGKEELLRRSLDRALDGLFAVLAEEQAVQGRAIDRLEH
                     VVRRSIEVLVDQLPHVTLLLRVRGNTRVERRALERRREFGRAITKLVTEAVEEGDLRA
                     GIDEGLTGRLLFGMINSVVDWYRPNGGSLTAGSLADAVISLTVEGWRQPGAQPRRKTR
                     "
     gene            complement(1343..1804)
                     /locus_tag="VFO47_12290"
     CDS             complement(1343..1804)
                     /locus_tag="VFO47_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8789516.1"
                     /translation="MTPRYRVDALSIEDGMEMASWRYPGPWAVFDSLEAPRPDEGWWA
                     VRDAGGRLTGFCCFGEAARVPGLAADPGRLDVALGLRPDLVGRGLGPELVRTVVAHAE
                     RVAQGRRLRCVVAISNEAGRRAAETAGFAVTGAHEVPGGAAVASYLVFLRG"
     gene            complement(1814..>2341)
                     /locus_tag="VFO47_12295"
     CDS             complement(1814..>2341)
                     /locus_tag="VFO47_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8789517.1"
                     /translation="WREAELLSGRLDRDRFASLLDNLVTPDVDEWFLCGPLELTETVQ
                     GTLVERGFDPDHIHRELFHVATGPARRQPPAAAPTDAETGAKVTVMLDGRGTSFTLPP
                     GTEPILDAALRLRGDAPYACKNGVCGTCRAKLVEGKVEMAQNFALERDELERGYVLAC
                     QSYPAAGEVTLDFDQ"
BASE COUNT          330 a          867 c          849 g          295 t
ORIGIN      
        1 agctgcgcgg ggtgacccag aagccgtgga tcaggacgat ggtgtcaggg gcggtcatgg
       61 cgtctccttc cctcgatgcg tgagtaggaa catcaaactc acagatccgg gtacgctaac
      121 acggtgagtc cagaaaccaa accaaccacc agggtctgct cgatcaccga cgccctctcg
      181 atcgtcggcg accgctacgc cctgctggtg gcccgcgagc tgcgctacgg gctgacccgc
      241 ttccaggaca tcgcggccag caccggggcc ccccgcgacg tgctcacctc gcggctgcgc
      301 aagctggagc aggccggcgt catcgagcgc cggccctact gcgagcaccc gccgcgctac
      361 gagtacctgc tcaccgacgc cggccgggac ctccagccga tcctgctggc cctcaaggag
      421 tggggcgacc gccactgcaa ccccggggcc gagccgatcg tgttccagca cacctgcggc
      481 gccgacttcc accccctgac cgtctgcgcg gcctgccggg gccccctccg ggacggcgac
      541 ctgaccgtca ccggtggcac ccacccggtc caggcgacca tgtgacgccg gcgaccgtgg
      601 tgctggccgc gtcgtggcct tcatccgggt ccaggagctg tgcctgggtc gatcgaacgg
      661 tcggtcggta agatggcccc cgaccggcga ctgagaggcg gatgcgcgag atggccacgg
      721 caggagggag gcgtcccccg gcgagccggc gggcgggcgg gcgccgctcc cagccgtacg
      781 acgcggactc gctgctggag gtcgcggtca aggtcttcac cgagcgcggc tacgacggca
      841 ccagcatgga ggacctggcc cgcgcggccc ggatcaccaa gtcgtcgttc tactaccacg
      901 tcagcggcaa ggaggagctg ctgcgccggt cgctcgaccg ggccctcgac gggctgttcg
      961 ccgtgctcgc cgaggagcag gcggtgcagg gacgggccat cgaccggctc gagcacgtgg
     1021 tccgccgctc gatcgaggtc ctggtcgacc agctgcccca cgtcacgctg ctgctgcggg
     1081 tccgcggcaa caccagggtc gagcgccggg cgctggagcg gcgacgcgag ttcgggcggg
     1141 ccatcaccaa gctggtcacc gaggccgtcg aggagggcga cctccgggcc ggcatcgacg
     1201 agggcctgac cggccggctg ctgttcggga tgatcaactc ggtggtcgac tggtaccgcc
     1261 ccaacggcgg cagcctcacc gccgggtcgc tggccgacgc cgtcatcagc ctgaccgtcg
     1321 aggggtggcg ccagccgggc gctcagccgc ggaggaagac caggtaggag gcgacggcgg
     1381 cgccgcccgg gacctcgtgg gcgccggtca cggcgaaccc ggccgtctcg gcggcccggc
     1441 gcccggcctc gttcgagatg gcgaccacgc agcggagccg ccgtccctgg gccacccgct
     1501 cggcgtgcgc caccaccgtc cggacgagct ccggtccgag gccccgcccg accaggtcgg
     1561 gacgcagccc cagggccacg tcgagccggc ccgggtcggc cgccagcccg gggacgcggg
     1621 ccgcctcgcc gaagcagcag aaccccgtga gccggccgcc ggcgtcccgg accgcccacc
     1681 agccctcgtc gggccggggc gcctccagcg agtcgaacac ggcccagggc cccgggtagc
     1741 gccaggaggc catctccatc ccgtcctcga tggagagcgc gtccacgcgg taccgcggcg
     1801 tcatctccct gcctcactgg tcgaagtcga gggtgacctc cccggccgcc ggataggact
     1861 ggcaggccag gacgtagccg cgctcgagct cgtcgcgctc cagggcgaag ttctgggcca
     1921 tctccacctt gccctcgacg agcttggccc ggcaggtccc gcagacgccg ttcttgcagg
     1981 cgtagggggc gtcgccgcgc agccgcaggg ccgcgtccag gatcggctcg gtgccgggcg
     2041 gcagggtgaa gctggtcccc cgcccgtcca gcatcaccgt caccttggcg ccggtttcgg
     2101 cgtcggtggg ggcggccgcc ggcggctgcc gcctggccgg cccggtggcg acgtggaaca
     2161 gctcgcggtg gatgtggtcg ggatcgaacc cgcgctcgac cagggtgccc tgcaccgtct
     2221 cggtgagctc cagcgggccg cacaggaacc actcgtccac gtcgggggtg acgaggttgt
     2281 cgagcaggga ggcgaagcgg tcacggtcga ggcggcccga cagcagctcg gcctcgcgcc
     2341 a
//
LOCUS       DASSWR010000732         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1157817, whole genome shotgun sequence.
ACCESSION   DASSWR010000732 DASSWR010000000
VERSION     DASSWR010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157817"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFO47_12300"
     CDS             complement(<1..390)
                     /locus_tag="VFO47_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789518.1"
                     /translation="MPALALANAGPLLDRLRPALLFSDVDGTLVGRDGSLLADLDGRP
                     TLAAAEALVAAHAAGLEVVLVSGRTAAQLRETGRLLGTRDAIAELGTVLVRGREVELV
                     WGEAPRDLGDCPAAALERSGALATVLEH"
     gene            complement(392..1387)
                     /locus_tag="VFO47_12305"
     CDS             complement(392..1387)
                     /locus_tag="VFO47_12305"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET8789519.1"
                     /translation="MTDIDTWEWFGNRTFHHSQFESPAKLRAAKERLGVTVSVCLPTR
                     DEAATVGGIVRNLRRNLVERARLVDEIVVMDAGSGDDTAAIARAEGARVFLEHDVLPG
                     EGPGSGKGEALWKSLHACEGDLLCFIDADIRNFHARFVYGLLGPLLLDPEIQYVKAFY
                     ERPIRERNALRATGGGRVTELLARPLINLLWPELAGIVQPLSGEYAGRRETLEQVPFF
                     SGYGVELGLLVDIAARFGVDAIAQVDLDRRVHRNQDMQALGRMSFGILQSAFLRLAEE
                     GRSAPGNFATTLVQFSNQQYEYQPEPREIVTSRRPPIVTVDDYRRRRADPVAASE"
     regulatory      1511..1619
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1682..2944
                     /gene="thrC"
                     /locus_tag="VFO47_12310"
     CDS             1682..2944
                     /gene="thrC"
                     /locus_tag="VFO47_12310"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET8789520.1"
                     /translation="MTDQTLDHVILRCRGCQTAYPAALEYACSRCLGPLDPEYDAAEV
                     AKRLSREAIEAGPRSIWRYAPLLPASPGPGDLAPGLTPLVPAPRLAEALGVPGPLFLK
                     DDTRNPTNSFKDRVVAVAVARARAFGLSTVACASTGNLAGATAAAAAAQGLECVVVIP
                     ADLEAGKVASAAVFGATVVAVEGSYDQANRLSMQAVEAFGWGFVNVNLRPWYAEGSKT
                     VGLEVAEQLGWSLPEHVVVPIASGALLTKVHQGFGALADHGLVERTPVRVHGAQSVGC
                     SPVATAFAAGADEVTPVRPSGIVKSLAIGDPADGAEALATVRGSGGRVEAIDDEEVVE
                     AIGLLARTTGVFGETAAGVTVATVGRLAAGGAFRPGERVVALITGHGLKTREVLDGRA
                     GATVTVRPNIDALSEALAPVRPHLAVPG"
     gene            complement(2941..>3130)
                     /locus_tag="VFO47_12315"
     CDS             complement(2941..>3130)
                     /locus_tag="VFO47_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789521.1"
                     /translation="PLAGSDAEAELENQLTLLEGLANAELRGAVLPNLPAGDEEETWR
                     QALITVVAGFSAGGTAAG"
BASE COUNT          398 a         1188 c         1149 g          395 t
ORIGIN      
        1 atgctccagc acggtggcca gggcgcccga ccgctccagg gccgcggccg ggcagtcgcc
       61 caggtcgcgc ggggcctcgc cccagaccag ctcgacctcc cggccccgga ccagcaccgt
      121 gcccagctcg gcgatggcgt cgcgggtccc gagcaggcgg cccgtctccc gcagctgggc
      181 ggccgtgcgg cccgagacga gcaccacctc cagcccggcc gcgtgggcgg cgaccagggc
      241 ctcggcggcg gccagggtgg ggcggccgtc caggtcggcc agcagcgacc cgtcccggcc
      301 gaccagcgtc ccgtcgacgt cggagaagag cagggccggg cggagccggt ccagcagcgg
      361 cccggcgttg gccagggcca gcgccggcac gctactcgga cgcggcgacg gggtcggccc
      421 gccgccgccg gtagtcgtcg acggtcacga tcggcgggcg ccgggaggtg acgatctcgc
      481 gcggctccgg ctggtactcg tactgctggt tggagaactg caccagggtg gtggcgaagt
      541 tccccggggc cgagcggccc tcctcggcca gccgcaggaa cgccgactgg aggatgccga
      601 agctcatccg gccgagggcc tgcatgtcct ggttgcggtg gacgcgccgg tccaggtcga
      661 cctgggcgat ggcgtcgacc ccgaagcggg cggcgatgtc gaccagcagg cccagctcga
      721 ccccgtagcc ggagaagaac ggcacctgct cgagggtctc gcggcgcccg gcgtactcgc
      781 ccgacagcgg ctgcacgatg ccggccagct ccggccagag caggttgatg agggggcggg
      841 ccagcagctc ggtgacccgg ccgccgccgg tggcccgcag ggcgttgcgc tcccggatgg
      901 gccgctcgta gaacgccttc acgtactgga tctccgggtc gagcagcagg gggccgagca
      961 gcccgtagac gaagcgggcg tggaagttgc ggatgtcggc gtcgatgaag cagagcaggt
     1021 cgccctcgca ggcgtggagg ctcttccaga gggcctcgcc cttgcccgac cccgggcctt
     1081 cgccgggcag gacgtcgtgc tcgaggaaca cccgggcccc ctcggcccgg gcgatggccg
     1141 cggtgtcgtc gccggagccg gcgtccatga ccacgatctc gtcgaccagg cgggcccgct
     1201 cgaccaggtt gcggcggagg ttgcggacga tgccgccgac ggtcgcggcc tcgtcccggg
     1261 tcgggaggca gaccgagacg gtcactccga gccgctcctt ggccgcgcgc agcttggccg
     1321 gcgactcgaa ctggctgtgg tggaaggtcc ggttgccgaa ccactcccag gtgtcgatat
     1381 cggtcatcgg ttctcccgat cgctggccgc tccggtgcgg ccatcgccgt tatctctccc
     1441 cctccggtgt tgcagccaag cagcgtgcca gggcaagctg gcagaccaca acttcatagg
     1501 tagtccaggc gacgcatcca gaggggcgga gggaccggcc cgacgaagcc ccggcaacct
     1561 cctccggacg aggaaggtgc cacttccggc ctcgccatcg tggcgaggga agatgcgccg
     1621 agcggttgac cgaccgcggc gcgacgtcgc cccacgccga gctcggaggt cgccgaccgc
     1681 catgaccgat cagaccctcg accacgtcat cctgcgctgc cgtggctgcc agaccgccta
     1741 ccccgccgcc ctcgagtacg cctgttcgcg ctgcctgggc ccgctcgacc ccgagtacga
     1801 cgccgcggag gtcgccaagc gcctgagccg ggaggcgatc gaggccgggc cgcggtcgat
     1861 ctggcgctac gccccgctgc tgccggccag ccccggcccc ggcgacctcg cccccggcct
     1921 gaccccgctg gtgccggcgc cccgcctggc cgaggcgctg ggcgtgcccg gccccctgtt
     1981 cctcaaggac gacacccgca accccaccaa ctccttcaag gaccgggtgg tggccgtggc
     2041 cgtggccagg gcgcgggcgt tcggcctgtc caccgtggcc tgcgcctcca ccgggaacct
     2101 ggccggggcc accgcggccg ccgccgccgc ccagggcctg gagtgcgtgg tcgtgatccc
     2161 ggccgacctg gaggccggca aggtcgcctc ggccgccgtc ttcggggcca ccgtggtggc
     2221 cgttgagggc tcctacgacc aggccaaccg gctctccatg caggccgtgg aggcgttcgg
     2281 ctggggcttc gtcaacgtca acctgcgccc ctggtacgcc gagggctcca agaccgtcgg
     2341 cctggaggtg gccgagcagc tcggctggtc gctgcccgag cacgtggtcg tcccgatcgc
     2401 ctccggcgcc ctgctcacca aggtccacca gggcttcggc gccctggccg accacggcct
     2461 ggtcgagcgg acgccggtca gggtccacgg cgcccagtcg gtcggctgca gcccggtggc
     2521 cacggccttc gcggccgggg ccgacgaggt caccccggtg cggcccagcg gcatcgtcaa
     2581 gtccctggcc atcggcgacc cggccgacgg ggccgaggcc ctggccacgg tccgcgggag
     2641 cggcggccgg gtcgaggcca tcgacgacga ggaggtggtg gaggcgatcg ggctgctggc
     2701 ccgcaccacc ggcgtgttcg gcgagaccgc cgccggggtg accgtggcca ccgtcggccg
     2761 cctggccgcc gggggcgcgt tccgccccgg cgagcgggtc gtcgccctga tcaccggcca
     2821 cggcctcaag acccgcgagg tcctcgacgg ccgcgccggg gccaccgtca ccgtccgccc
     2881 caacatcgac gccctctccg aggccctggc cccggtccgc ccccacctgg ccgtgcccgg
     2941 ctagccagcc gcggtcccgc cggcgctgaa gcccgcgacc acggtgatga gcgcctgccg
     3001 ccaggtttcc tcctcgtcgc cagcaggcag gttcggcagc acggcaccgc gcagctcggc
     3061 gttggcgagc ccctcgagca aggtaagctg gttctcgagc tcggcttcag cgtcgcttcc
     3121 ggcgagaggt
//
LOCUS       DASSWR010000733         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_366733,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000733 DASSWR010000000
VERSION     DASSWR010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366733"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFO47_12320"
     CDS             complement(<1..868)
                     /locus_tag="VFO47_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8789522.1"
                     /translation="MRRIRLGGAAVAACLALLLAACGGGDDALEGGTQPNEQSSVTVG
                     STNFSEQLILAQMYAAVLEKAGVEVEVRPNLGAREVVFPALEKGEIDLLPEYNGSVLS
                     FLDTDATQTTSEEVNTALTPLLDQKGLVALQQSPAEDKDGWAVTKETADKYGLAKVSD
                     LKGKASQLVVGGPPELETRPAGLPGLKKVYGIEDFKEFKALDAGGPLTTSALNKGDID
                     VGRVFTTQGAIAENGWVVLEEDKPLQPAQNIVPIGRKDAITDQVTSALNALSAKITTE
                     ELTNLNKLVDVDK"
     gene            complement(915..1622)
                     /locus_tag="VFO47_12325"
     CDS             complement(915..1622)
                     /locus_tag="VFO47_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8789523.1"
                     /translation="MNVVTEVLAWLTDPAQWSGPDGIPVRTLQHLWYSLLATAVAAAI
                     ALPVGIFIGHTGRGATFAVNLTNLGRAIPSLGIIILMFTVFGFGVAPILITLFALAIP
                     PIVTNSYIGVHSVDPDVRQAAEGMGMRGRQVLWQVELPMAMPLIMAGIRTSAVQVVAT
                     ATLAAYVGLGGLGRYLIDGLAQRDLAEVVGGAILVAVLSLLTELVLGRVQALVVSKGL
                     AERGADAAVRAEVEKAA"
     gene            complement(1629..2306)
                     /locus_tag="VFO47_12330"
     CDS             complement(1629..2306)
                     /locus_tag="VFO47_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8789524.1"
                     /translation="MSDGPAPVLAAEVIRWSWVGDHLDDIRDYLIQHVQLTVLAVLLG
                     LLLAFPLSLAAIRWPRLYGPVLGFTGMLFTIPSLALFILLIPFTGLSIRTSLIGLTMY
                     TLLILVRNIVEGLRGVDHDVREAAQAMGYTRARQLFRVELPLALPVIMAGIRIATVTT
                     IGLVTVTALIGQGGLGRLFIDGFTLNFTTPIIIGIVLSALLAFAADLALVGLQRQLTP
                     WARSRGR"
     gene            complement(2303..>2769)
                     /locus_tag="VFO47_12335"
     CDS             complement(2303..>2769)
                     /locus_tag="VFO47_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8789525.1"
                     /translation="RQGGHLEQYAPPDELLAEPASDFVADFLGAERGLKRLALIPVSA
                     VKAEAGPVVAPDDDGRAALAAAEAYGSDGVVVVDGDRRLLGWAHAGEAVERGRVGDAE
                     IRPFQLQVQADDSLRAALNAMVQSRTGVAVRVSDGDRYEGIVTLELLSKEIS"
BASE COUNT          406 a          937 c         1034 g          392 t
ORIGIN      
        1 gcttgtccac gtcgaccagc ttgttgaggt tggtcagctc ctcggtggtg atcttcgccg
       61 agagggcgtt gagggccgag gtgacctggt cggtgatggc gtccttgcgc ccgatgggga
      121 cgatgttctg ggccggctgg agcggcttgt cctcctccag caccacccag ccgttctcgg
      181 cgatggcgcc ctgggtggtg aacacccggc cgacgtcgat gtcccccttg ttgagggccg
      241 aggtggtcag gggcccgccg gcgtccaggg ccttgaactc cttgaagtcc tcgatgccgt
      301 agaccttctt gaggccgggg aggccggccg gccgggtctc cagctcgggc ggcccgccga
      361 cgaccagctg gctcgccttg cccttgaggt cggacacctt ggccaggccg tacttgtcgg
      421 cggtctcctt ggtgacggcc cagccgtcct tgtcctcggc cgggctctgc tggagggcga
      481 ccaggccctt ctggtcgagc agcggcgtga gggcggtgtt gacctcctca gacgtggtct
      541 gggtggcgtc ggtgtccagg aacgacagca ccgacccgtt gtactcgggc agcaggtcga
      601 tctcgccctt ctccagggcc gggaagacga cctcgcgcgc ccccaggttc ggccggacct
      661 cgacctcgac gccggccttc tcgaggacgg cggcgtacat ctgggccagg atgagctgct
      721 cggagaagtt ggtcgagccg acggtcacgc tcgactgctc gttgggctgg gtaccgccct
      781 cgagcgcgtc gtctccgccg ccgcaggcgg ccaggagcag cgccaggcag gccgcgaccg
      841 cggccccacc aagccggatc cttcgcatgg acgatcttcc tttccttggg gactgggacc
      901 ttgtcgtccc gggactatgc ggccttctcg acctcggcgc ggacggccgc atctgcgccc
      961 cgctcggcca ggcccttgga gaccaccagg gcctggacgc gaccgagcac cagctcggtg
     1021 agcagcgaca gcacggcgac caggatggcc ccgccgacca cctcggccag gtcgcgctgg
     1081 gcgagcccgt cgatcaggta gcggccgagc ccgccaaggc cgacgtaggc ggccagggtt
     1141 gcggtggcca ccacctgcac cgccgaggtg cggatgccgg ccatgatcag cggcatggcc
     1201 atcggcagct cgacctgcca caggacctgc cggccgcgca tgcccatgcc ctcggcggcc
     1261 tggcggacgt cggggtcgac cgagtggacg ccgatgtagc tgttggtcac gatcgggggg
     1321 atggccagcg cgaacagcgt gatcaggatc ggggcgacgc cgaagccgaa gacggtgaac
     1381 atcaggatga tgatgcccag cgacgggatc gcccggccaa ggttggtcag gttgacggcg
     1441 aaggtggccc cgcgccccgt gtgcccgatg aagatgccga cgggcagggc gatggcggcg
     1501 gcgacggcgg tggccagcag cgagtaccag aggtgctgga gggtgcggac cgggatgccg
     1561 tccggcccgc tccactgggc cgggtcggtg agccaggcca gcacctcggt gacgacgttc
     1621 accggcggct acctcccccg gctccgggcc cagggggtga gctggcgctg caggccgacc
     1681 agggccaggt cggcggcgaa ggcgagcagg gccgacagca cgatcccgat gatgatgggg
     1741 gtggtgaagt tcagggtgaa gccgtcgatg aacagccgcc ccagcccgcc ctggccgatc
     1801 agggcggtga ccgtgaccag cccgatggtg gtgacggtgg cgatgcggat gccggccatg
     1861 atcaccggca gggcaagggg cagctcgacc cggaagagct ggcgggcccg ggtgtagccc
     1921 atcgcctggg cggcctcgcg cacgtcgtgg tcgacccccc gcagcccttc gacgatgttc
     1981 cggaccagga tcagcagggt gtacatggtc agcccgatca gcgaggtcct gatcgacagc
     2041 ccggtgaacg ggatcagcag gatgaacagg gccagcgacg ggatcgtgaa cagcatcccg
     2101 gtgaagccga ggacgggccc gtacagccgc ggccagcgga tcgcggccag ggacagcggg
     2161 aaggccagca gcagcccgag cagcacggcc aggacggtga gctggacgtg ctggatgagg
     2221 tagtcgcgga tgtcgtcgag gtggtcgccg acccacgacc agcggatcac ctcggccgcc
     2281 aggaccgggg ctgggccgtc gctcaactga tctccttgga gagcagctcc agggtgacga
     2341 tgccctcgta gcggtcgccg tcgctgaccc gcacggccac gccggtgcgg gactggacca
     2401 tggcgttgag ggcggcccgc agcgagtcgt cggcctggac ctggagctgg aagggacgga
     2461 tctcggcgtc gccgacacgg ccacgctcga ccgcctcgcc ggcgtgggcc cagccgagca
     2521 gccgccggtc gccgtccacc accaccaccc cgtcgctgcc gtaggcctcg gccgcggcca
     2581 gggcggcgcg gccgtcgtcg tcgggcgcga ccaccgggcc ggcctcggcc ttgaccgccg
     2641 acaccgggat cagggccagg cgcttgaggc cgcgctcggc cccgaggaag tcggccacga
     2701 agtcgctggc cggctcggcc agcagctcgt ccgggggcgc gtactgctcc aggtggccgc
     2761 cctggcgga
//
LOCUS       DASSWR010000734         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_931740,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000734 DASSWR010000000
VERSION     DASSWR010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931740"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..377
                     /locus_tag="VFO47_12340"
                     /pseudo
     CDS             1..377
                     /locus_tag="VFO47_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            457..2100
                     /locus_tag="VFO47_12345"
     CDS             457..2100
                     /locus_tag="VFO47_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8789526.1"
                     /translation="MGRYDAIVVGGGHNGLVAAAYLARAGHRTLVCEAREVVGGAAVT
                     ERPFGPDYKVTSLSYVISLMPQGIVRDLDLHRHGYKVYPQGPYFAPHARGTSLQLSND
                     PVKRRQEIAKFSGRDADAEERWDAWMAGLADVLGPLLSAIPPKVGSRRPADLASQAQL
                     VWRLRGLDTRKAADVTRLFTLSIADLLEEFFESPELQAVHAVSGVIGTWAGPRAPGTA
                     YVMAHHHIGDVGDGHLGSWGFAEGGMGGVSNAIAGAARSFGAEIRTSAPVRRITTSNG
                     RATGVVLEGGQELAADVVVAATHPRITFLEQVDRSELPGDFVTDIERWKTRSGTVKVN
                     LATERLPRFTCRPEFDPEIHGGTIVLAESLDDMEGAFQDAAAGRPSALPFADVCIPSV
                     FDKTLAPEGHHIVSMFTQWVPHTWAGKPMGEELEAHADRVVARMERVAPGFTDSILFR
                     QVIGPYQMEHEYGLVGGNIFHGELSASQLFHTRPAAGYADYRTPVRGLYQAGSATHGG
                     GGVTGIPGRNVVRQIEKDRRKDERRQKVRARLPIPTRSG"
     gene            2192..3310
                     /locus_tag="VFO47_12350"
                     /pseudo
     CDS             2192..3310
                     /locus_tag="VFO47_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            3370..4524
                     /gene="solA"
                     /locus_tag="VFO47_12355"
     CDS             3370..4524
                     /gene="solA"
                     /locus_tag="VFO47_12355"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET8789527.1"
                     /translation="MYDAVVVGLGALGSAAAWVLARSGARVLGLEQFELGHHRGASHD
                     HARIIRRSYHTPGYVRLAGLAYRAWAELEWEAEERLVVTTGGLDLFPPGSAIPPGPYR
                     ASLEACGVPYQWLDADEVMRRWPAFRLDDGVGGLWQADAGMVPAARGTRLMQRLAAGY
                     GATLRDRTPVTAVRDLGGSVEVATADATYRAGRLLLCADAWTGDLLAQLGAGLPLVVT
                     QEQFSYYRPADPDAFAVGRFPVWIWMDDPSFYGFPVWPGADLKAAQDVGGHQVTATTR
                     SFDPDPAASDRLAGFLRARLPAAAGAHARTATCLYTLTPDRDLVLGPLPGHDRVLVAL
                     GAAHGFKFAPLVGRVLADLALHDGTDVDIGPFALDRPSLTSARPEANYLV"
     gene            complement(4550..4684)
                     /locus_tag="VFO47_12360"
     CDS             complement(4550..4684)
                     /locus_tag="VFO47_12360"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8789528.1"
                     /translation="MRRSIAAAVERAVAAGATKVREVDERDEHWVVLQDPEGNEFCMH
                     "
BASE COUNT          567 a         1870 c         1642 g          624 t
ORIGIN      
        1 gcgagatcct ggccctgctg ctcggcctgg tccgcgacct cggcctgtcc atcctggtcg
       61 tcacccacga cctcggcctg gcctggaaca tcgccgaccg gctggccgtc atgtacctgg
      121 gccgcatcgt cgagcagggc ccgaccgagg ccgtgctggc cgacccccgc cacccctaca
      181 cccgggccct gctgtcggtg gtgcccgagg cccaccggca ggagacccag atcctcgccg
      241 gcgagccgcc cgacccgacc cgggtcccgg acggctgccg cttccacccc cgctgcccgg
      301 ccctggcctc cggcgcggcc gccgaggcgg gcgtggccga ccgctgcgtc gccgaggcgg
      361 tgcccatcct gcgcggcgtg gccgagcatc atgctgcttg cttcctgact gacgaatcag
      421 ttaaaaagga gcgtggggac cgggtcggag gtaggcatgg ggcggtacga cgcgatcgtg
      481 gtcgggggcg ggcacaacgg gctggtcgcg gccgcctacc tggccagggc ggggcaccgg
      541 acgctggtct gcgaggcccg cgaggtggtc gggggcgccg ccgtcaccga gcggccgttc
      601 gggcccgact acaaggtgac ctcgctgtcg tacgtgatca gcctcatgcc ccaggggatc
      661 gtgcgcgacc tcgacctcca ccgccacggc tacaaggtct acccccaggg cccctacttc
      721 gccccccacg cccgcggcac ctccctccag ctctccaacg acccggtgaa gcgccgccag
      781 gagatcgcca agttctcggg ccgcgacgcc gacgccgagg agcgctggga cgcctggatg
      841 gccggcctgg ccgacgtgct cgggccgctg ctgtcggcca tcccgcccaa ggtcggctcc
      901 cggcgccccg ccgacctggc ctcccaggcc cagctggtgt ggcggctgcg cggcctcgac
      961 acccgcaagg cggccgacgt cacccggctg ttcaccctga gcatcgccga cctgctggag
     1021 gagttcttcg agtcgcccga gctccaggcc gtccacgccg tcagcggcgt catcggcacc
     1081 tgggccggcc cccgcgcccc cgggaccgcg tacgtgatgg cccaccacca catcggcgac
     1141 gtcggcgacg gccacctggg cagctggggc tttgccgagg gcggcatggg cggggtcagc
     1201 aacgcgatcg ccggcgcggc ccgctccttc ggggccgaga tccgcacctc ggccccggtc
     1261 cggcgcatca ccaccagcaa cggccgggcc accggggtcg tcctggaggg gggccaggag
     1321 ctggccgccg acgtggtcgt ggccgccacc cacccccgga tcaccttcct ggagcaggtc
     1381 gaccgttccg agctgcccgg cgacttcgtg accgacatcg agcgctggaa gacccgcagc
     1441 ggcaccgtca aggtcaacct ggccacagag cggctgcccc gcttcacctg ccggcccgag
     1501 ttcgacccgg agatccacgg cggcaccatc gtcctcgccg agtcgctgga cgacatggag
     1561 ggcgccttcc aggacgcggc cgccggccgg ccctcggcgc tgccgttcgc cgacgtctgc
     1621 atcccctcgg tgttcgacaa gaccctggct cccgagggcc accacatcgt ctcgatgttc
     1681 acccagtggg tgccgcacac ctgggccggc aagccgatgg gcgaggagct ggaggcccac
     1741 gccgaccggg tggtggcccg catggagcgg gtcgcccccg ggttcaccga ctcgatcctg
     1801 ttccgccagg tgatcggtcc ctaccagatg gagcacgagt acgggctggt cggggggaac
     1861 atcttccacg gcgagctgtc ggccagccag ctgttccaca cccgcccggc cgccggctac
     1921 gccgactacc gcacccccgt ccgcggcctc taccaggccg ggtcggccac ccacggcggc
     1981 ggcggcgtca ccggcatccc cgggcgcaac gtggtccgcc agatcgaaaa ggaccgccgc
     2041 aaggacgagc ggcgccagaa ggtccgcgcc cgcctcccga tcccgacccg ctctggctga
     2101 gctcccctgg ggggtgtgga caacccggag aactccctcc cccggtcggc taccctcccg
     2161 accggggccc aagtccagga ggtgagcggt cttggccggc ctggagcgcg ccctgcccgc
     2221 atcctactac ctcgacgagg ccgccttccg gcgggaacgt gagcggctcc tctttggcga
     2281 ttggttctgt gccggacggg ccgaccaggt cgagcggccg ggatcgctcg tggtggtcga
     2341 cgtcgccggc gagagcatcc tgctggtccg gacccgggcg ggggagctgc gggcccacta
     2401 caacgtctgc cgccaccgcg gctcccaggt ggtgccgtgc ccgcccgcca cccccgggtg
     2461 gccccccggc cggcccggcc cgcaaggccg ggtcgctgcg ctgcccctac cactcctgga
     2521 cctaccggct ggagggcgac ctgctccggg cccctggagc gaggagatcg acggcttcga
     2581 ccagcaggcc ttcgggctcc acccggtggg ggtggcgaca tggggcgggt tcgtgttcct
     2641 gcatctgacc ccggccgagg ccgggcccct ggccgaccag ctgggccccg tgcccgagcg
     2701 gctgcgccgc tacccgctgg aggcgctggc cgtcgggcgg cggctcgtct accgggtcgg
     2761 ggccaactgg aagctggtcg ccgagaacta caacgagtgc taccactgcg ccggcgtcca
     2821 tcccgagctg tgccgggtcg tgcccgcgtt caggcgcggc ggggccgggc tggactggga
     2881 ccggggcgtc ccccaccgcg agggcgcctg gacgttcacg gccaccggcc agagcgaccg
     2941 ggcgccgttc ccgggcctgg acgacgacga gcgcacccgc cacaagggcg agctggtcta
     3001 ccccaacctg atgctgagcc tgtccgccga ccacgtcgcc gccttcaccc tgtggccgca
     3061 cgggccgggg tagaccacgg tcgtgtgcga gctgctgttc caccccgacg agcaggcccg
     3121 gcccggcttc gacccctcgg acgcggccga cttctgggac ctggtcaacc gccaggactg
     3181 ggccatgtgc gagagcgtcc agcgcggcat gtcgtcgcgc gcctattccg gcggctggtt
     3241 cgcccccatg gaggagccca gcctcgacat ccgccgctac gtcctggacc ggctgggaga
     3301 ggaggggtga cgggtcctgc cgggcgcaac gggccggcct gggcgccgcg cccccgaccg
     3361 gctgggccgt tgtacgacgc ggtggtggtc gggctggggg cgctggggag cgccgccgcc
     3421 tgggtgctgg ccaggtcggg ggcccgggtg ctggggctgg agcagttcga gctcgggcac
     3481 caccggggcg cctcccacga ccacgcccgc atcatccgcc gctcctacca cacccccggc
     3541 tacgtacgcc tggccggcct ggcctaccgg gcctgggcgg agctggagtg ggaggcggag
     3601 gagcggctgg tggtgaccac cggcggcctc gacctgttcc cgcccgggtc ggccatcccg
     3661 cccggcccct accgggccag cctggaggcc tgcggcgtgc cctaccagtg gctggacgcc
     3721 gacgaggtga tgcgccgctg gccggccttc cggctggacg acggcgtggg cgggctctgg
     3781 caggccgacg ccggcatggt cccggccgcc cgcggcaccc ggctcatgca gcgccttgcc
     3841 gccggctacg gcgccaccct gcgcgaccgc accccggtga cggccgtccg cgacctcggc
     3901 ggctcggtcg aggtggccac ggccgacgcc acctaccggg ccggccgcct gctgctctgc
     3961 gccgacgcct ggaccggcga cctgctcgcc cagctcggcg ccggcctccc cctggtcgtc
     4021 acccaggagc agttcagcta ctaccgcccg gccgacccgg acgccttcgc cgtcggccgc
     4081 ttcccggtct ggatctggat ggacgacccc agcttctacg gcttcccggt ctggcccggc
     4141 gccgacctca aggccgccca ggacgtcggc ggccaccagg tcacggccac cacccgctcc
     4201 ttcgaccccg acccggccgc ctccgaccgc ctggccgggt tcctgcgcgc ccgcctcccc
     4261 gccgccgccg gcgcccatgc ccgcaccgcg acctgcctgt acaccctcac cccggaccgc
     4321 gacctcgtcc tcggccccct ccccggccac gaccgcgtcc tggtcgccct cggcgccgcc
     4381 cacggcttca agttcgcccc cctggtcggc agggtcctgg ccgatctggc cctgcacgac
     4441 ggcaccgacg tcgacatcgg gccgttcgcc ctcgaccgcc cgtccctcac cagcgcccgg
     4501 cccgaggcga actacctggt ctgagccttg gcccggggcg gtcggccggt cagtgcatgc
     4561 agaactcgtt gccctcgggg tcctggagga cgacccagtg ctcgtcacgc tcgtcgacct
     4621 cgcggacctt ggtcgcgccg gcggcgacgg cccgctcgac ggcggcggcg atgctgcggc
     4681 gcaactcctc gggcggggtg ccg
//
LOCUS       DASSWR010000735         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_733375,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000735 DASSWR010000000
VERSION     DASSWR010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733375"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1050..1703
                     /locus_tag="VFO47_12365"
     CDS             1050..1703
                     /locus_tag="VFO47_12365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET8789529.1"
                     /translation="MATQADAIAAYLAELTVVEDDVLRHARERASAGGMPPVSADAGA
                     FLRFAASWVGAQAAVEIGSGAGYSGVWIARGLAPKGILTTIEADPGHQRLAKETYEEA
                     GVPSRIRAILGRALDVLPRLTDGGYDLAFLDAKKSEYPEYLEHALRLVRPGGVIIADN
                     VLWSGRVADPEVTDPDTEGLRAYARRIAADDRLDSVILTVGDGLAVSRVRRNEELEA"
     gene            1712..>2497
                     /locus_tag="VFO47_12370"
     CDS             1712..>2497
                     /locus_tag="VFO47_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789530.1"
                     /translation="MTTTYMGGRSGPPDGDERPLRGLPGGRARPRPFPPSPTPVDDGP
                     AGGTAGGDGDQPVERVPGGSKHGDRPARRPHGDGGGSGQGGNGGAVRPLRVVPGGAAP
                     PLRLIPGGAGAPTELGDGDGQAPDGVLTISPPLPVPQPAGGARRRSRPRRRWLAVVLA
                     LLLVAGLAATGRLAAERPRTGAPTAPATTVSPGSVPSGRPSPIVATIPTGGFSSAMAA
                     GAGALWVVGSDEVIRIDPATDTVAARVPVRATGSGPAAVAVGAG"
BASE COUNT          263 a          981 c          966 g          287 t
ORIGIN      
        1 atcccggccc tccttgccat ccggaaccac gcgggcagcg gcatccagcg ccggctcacg
       61 ctcggcccgg gggtcgcctc gcctcccagg ctccggcccc agatcggccg caccctccga
      121 tgccggttcg cggccagctt gccggtcaac ccggctccca gctcccgggc tcgggccgac
      181 cccaccctcc gatccgagtc cacgcccggc ctgcggatgg tcctggctcc cggactccgg
      241 gtccgatccg ccaccggacg ccggaaccgg gccgctccgg atctccgcgc cgggcggagg
      301 ctcggcgtcg cgaaccggcc cgcgcgcggc cgccggtagg gggtcgtcct ccgtcttcgc
      361 cggacccgtg tcggagtggg gcgacggtgg tgcctcggct ccggccggtc cggcgctggg
      421 agccggcgac gcctggccgg ccaccggagc cggtatccac acgggacccg ccaacggctg
      481 ggggtccggc gaccgctcgg agtccggcga ccgctcggag tccggcgacc gctcggggtc
      541 cggcgaccgt gcgggagcct gggcctccgc ccgccctggc gccggggcgc cctcggacgg
      601 ggccatggtc ggcgtgggag cggagcgaac ctcgccagcc ggttccggcg gagcggggct
      661 cggctgcggg gcctcggccg ggcgacgggc ggcgaacgag ccggccggtg ccggaggggc
      721 cagcggcgcg gcgtccgacc cccgcccctc gggcgatccg gcgccggaga gctgacccgg
      781 gtcggagggc gatccggatc cgggcggatc gaccggttct gggcggtccg ggtcgaggcg
      841 ccctggcccg ggggtggggt cggtcgttcg gtccacgcaa gctccctcgt gcggcccccc
      901 ggacgggcgt cgcgcccagc ctatcggggc gggagcgctt ctgcgacagg cgttgtccac
      961 aacctgggag ggtcccatac cgccggccgc cgcagagggt tacgctggac ccgtcccgat
     1021 cgcagccttg cagcttcaaa ggagtcgccg tggccaccca ggccgacgcc atcgccgcgt
     1081 acctcgccga gctgacggtg gtcgaggacg atgtcctgcg ccatgcccga gagcgggcgt
     1141 ccgccggtgg catgccgccc gtgtcggccg acgcgggggc gttcctgcgc ttcgccgcca
     1201 gctgggtcgg cgcccaggcg gcggtcgaga tcggctcggg cgccgggtac tccggggtct
     1261 ggatcgcgcg cggcctcgcg cccaagggca tcctcacgac catcgaggcc gacccagggc
     1321 accagcggct ggccaaggag acctacgagg aggccggcgt ccccagccgg atccgggcca
     1381 tcctgggccg cgccctcgac gtgctcccga ggctcaccga cggcgggtac gacctggcgt
     1441 tcctggatgc gaagaagtcc gagtaccccg agtacctgga gcacgcgctg cgcctggtgc
     1501 gacccggcgg ggtgatcatc gccgacaacg tgctgtggtc gggacgggtc gccgacccgg
     1561 aggtgaccga cccggacacc gaggggctgc gggcctacgc ccgccgcatc gccgcggacg
     1621 accggctcga ctcggtgatc ctcaccgtcg gcgacgggct ggcggtgtcc cgggtgcgcc
     1681 gcaacgaaga gctggaggcc tgaaccggct cgtgacgacg acgtacatgg gcgggcggag
     1741 cggcccgccg gatggcgacg agcggcccct ccgcggcctg cccggcggtc gtgcccgccc
     1801 acgcccgttc ccgcccagcc ccaccccagt cgacgacggc ccggccggcg ggaccgccgg
     1861 cggcgacggc gaccaacccg tcgaacgggt ccccggcggg tccaaacacg gcgaccgacc
     1921 cgcccggcgg cctcatggcg acggcggcgg gtcggggcag ggcgggaacg gcggggcggt
     1981 gcggccgctg cgggtcgtgc ccgggggtgc cgctccgccg ctgcggctga tcccgggcgg
     2041 ggcgggggcg ccgacggagc tgggtgacgg ggacggccag gcccccgacg gcgtcctgac
     2101 catctccccg cccctgcccg tgccccagcc tgccggcggg gcccgtcgcc gttcccggcc
     2161 aaggcggcgc tggctggcgg tggtcctcgc cctgctcctg gtggccgggc tggccgcgac
     2221 cggccggctg gccgccgagc gaccccgcac cggcgcgccc accgctccgg cgacgaccgt
     2281 ctcgcccggg tcggtcccct cgggccggcc ctcgccgatc gtggccacca tcccgactgg
     2341 cggcttctcc tccgccatgg ccgccggcgc cggggccctc tgggtcgtcg gcagcgacga
     2401 ggtcatccgc atcgacccgg ccaccgacac ggtggcggcc agggtcccgg tccgcgcgac
     2461 cggctccggc ccggccgccg tggccgtcgg ggcgggg
//
LOCUS       DASSWR010000736         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1099651, whole genome shotgun sequence.
ACCESSION   DASSWR010000736 DASSWR010000000
VERSION     DASSWR010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099651"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFO47_12375"
     CDS             complement(<1..1176)
                     /locus_tag="VFO47_12375"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET8789531.1"
                     /translation="MDDPGLRLLGHLLDASHNLAPDELVAAVTRAGGAVDAADVAIYL
                     VDYEQTLLVPLADGSDRTPLEIDATLAGRAFAALAVQEADTGAGRRLWLPLLDGAERL
                     GVLGLTLPRVDDALRKRCSWLAALVAEMILSKDQYTDNYSLTRRRQPMSPAAEMQWQL
                     LPPLTFVTPRVVIAGLLEPAYEVAGDAFDYALNGDTAHLAIVDPVGHDLTATVLAAVT
                     VGSYRHSRRAGLDLAATHAAMDETIATQFGGERFVTGQLAQLDCLTGRLQWVNAGHPL
                     PLLVRRAKVVDTLACHPVPPLGVGLGQPEIAAAALEPADRVEDGQVSGVAVEGVVKGV
                     PGHLVGRLQQPGDHHSRGHKGQRREQLPLHLGGRAHGLAAAGQGVVVGVLVLAQDHLG
                     "
     gene            1476..1925
                     /locus_tag="VFO47_12380"
     CDS             1476..1925
                     /locus_tag="VFO47_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET8789532.1"
                     /translation="MAGRPDSAAPANRHPSQLLALAIGVIYTLIGLAGFLVTGFDNFA
                     SETDKTLVGFEINPLHNIVHLAIGLAGLALWRRLDTARTYGWLLAAGYGLAFLYGLFA
                     AGNSDINFLSLNGADNGLHLVSAVAGVAIALWPAQRTAPAGRAERSS"
BASE COUNT          304 a          773 c          760 g          324 t
ORIGIN      
        1 gccgagatga tcttgagcaa ggaccagtac accgacaact actccctgac ccgccgccgc
       61 caacccatga gcccggccgc cgagatgcaa tggcagctgc tcccgcctct gacctttgtg
      121 accccgcgag tggtgatcgc cgggctgctg gagccggcct acgaggtggc cggggacgcc
      181 tttgactacg ccctcaacgg cgacaccgct cacctggccg tcctcgaccc ggtcggccgg
      241 ctcaagggcc gcggcggcga tctccggctg gccgaggccg acgcccagcg gcgggaccgg
      301 gtggcaggcc agcgtgtcga ccaccttggc tcgtcgcacc agcagtggca ggggatggcc
      361 ggcgttgacc cactggagcc ggccggtcag gcagtcgagc tgggcgagct gaccggtgac
      421 aaagcgttcg ccgccgaact gggtggcgat ggtctcgtcc atggctgcgt gagtggcggc
      481 caggtcgagg ccggcacggc ggctgtgccg gtagctgccc acggtgaccg cggccaggac
      541 ggtggcggtc aggtcgtggc cgaccgggtc gacgatggcc aggtgagcgg tgtcgccgtt
      601 gagggcgtag tcaaaggcgt ccccggccac ctcgtaggcc ggctccagca gcccggcgat
      661 caccactcgc ggggtcacaa aggtcagcgg cgggagcagc tgccattgca tctcggcggc
      721 cgggctcatg ggttggcggc ggcgggtcag ggagtagttg tcggtgtact ggtccttgct
      781 caagatcatc tcggcgacca gggcggccag ccagctgcac cgcttccgca gcgcgtcgtc
      841 gaccctcggg agggtgaggc ccaggacgcc cagccgctcg gcgccgtcca gcagcggcag
      901 ccacagccgc cgtcccgctc cggtgtcagc ctcctggacg gcgagggcgg caaaggcccg
      961 cccggccagg gtggcgtcga tctccagcgg cgtccggtcg ctgccatcgg ccagcggaac
     1021 cagcagggtc tgctcgtagt cgaccaggta gatggcaaca tcggcggcgt ccacggcccc
     1081 tccggcccgc gtgaccgcag cgacgagctc gtcaggggcc aggttgtggg aggcgtccag
     1141 caggtgacca aggaggcgca ggcccgggtc atccatccct ccacctccgg atcgcgggtc
     1201 ggcgagcacc gcccagaggc gcacgcattc catccgagcc gacacgcccg ccgccgagcg
     1261 cacacaagtc tgcgtccggg cagtggggcc gtccagcccg tgaactgccg gtggccgcga
     1321 ggttgagagc gtgctcggtg gcccgctctt gtcgctgggt ggactgggcg gtttgtgacg
     1381 gccagacctg gctacccacc tgtcggcccg gcagaaggcc gggtcgactg aaagggccca
     1441 gaccccgtct tttccaccac ggaggcatcc ttctcatggc aggacgtcct gattccgccg
     1501 cgcccgcgaa ccggcacccg agccagctgc tggccctggc catcggcgtc atctacaccc
     1561 tgattggcct tgccggattc ttggtcaccg gcttcgacaa cttcgcgtcc gagaccgaca
     1621 agaccctggt cggcttcgag atcaaccccc tgcacaacat cgtccacctg gccattggcc
     1681 tggccgggct ggcgttgtgg cggcggctgg acacggccag gacctacggt tggctgctgg
     1741 ccgccggcta cggcctggcg ttcctctacg gcctgttcgc cgccggcaac tccgacatca
     1801 acttcctgtc cctcaacggc gccgacaacg gcctccacct cgtcagcgcc gtggccgggg
     1861 tggccatcgc cctgtggccg gcgcagcgga cggctccggc cggccgagcc gagcgcagct
     1921 cctgacccga acggccagcc ggccaacgcg gtgcgggaac tgcggctctg ggccgccgcg
     1981 gcggccgcgc cggccgtcgc ggcggggttc tccctgttgg ccggcatgcg tcgccgacgc
     2041 tctctacacc cggtcggagc cggctaccgg ggttggttcg tcgtcgatga ggagcaccct
     2101 gaccggcagg atgttcccct gttccagcca gggagggcag cgactgccgt cctccgcttc
     2161 t
//
LOCUS       DASSWR010000737         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_225767,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000737 DASSWR010000000
VERSION     DASSWR010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225767"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFO47_12385"
     CDS             <1..192
                     /locus_tag="VFO47_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET8789533.1"
                     /translation="GVAVVNEIKVRTARLLVARGGMPPVLTSGSVVGNEESARLFDAA
                     YDEHARRLARVLAGREEHV"
     gene            189..1250
                     /locus_tag="VFO47_12390"
     CDS             189..1250
                     /locus_tag="VFO47_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8789534.1"
                     /translation="MRRPRWMRAGILIAAMALAVTACGGGDDDAASGGSGAGSGQSGN
                     FVIGVSNTLAGNGWREQMICSVKAQALASGKVSKVIAISKNGGPNEQIQDLQNLISQG
                     ADAIIVNPSDREKLNPVIQQATQKKIVVVAVDQAVSEPTAYVATNDQVAYGRLGAEWL
                     AEQLGGKGNVLYMRGIDGVPADADRDKGFQEVMAQNPDIKIKEVFTGWDFTKGGDIAV
                     QELGSGNYDGVWTSGIDYTVVNAFKTVGKEPVPVVGADNNEFIQQLLDGSPGAAVTNP
                     AVIGGVGTAIALDVLSGKTVKRETLLTPEVWDAEQDKATLEANSFPDREATFSSAVSV
                     KPFTTYTNEQLFACKGPGE"
     gene            1269..>2522
                     /locus_tag="VFO47_12395"
     CDS             1269..>2522
                     /locus_tag="VFO47_12395"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET8789535.1"
                     /translation="MSQPLLQATAVAKAFGPVVALREVDLSVSPGEVHALLGANGAGK
                     STLVKILTGVLHHDSGTVAVNGAQVELRSPTEARGRGLAPVYQDPAMIGDLTVSQNLR
                     LTGADPAKVRHELAEMDLDGLDLDEQVRDIPLPFLRMLDLARALAFDPQLLVLDEITA
                     ALPPDLSDRVFAIMTRWKERNRSVLFISHRLAEVRQHCDMCTVLRDGRDVASFHPGSE
                     GGESQIVAAMLAEAGAGVRDTARERAARAEGERTQRLVVRDLLIDRQDAGVSFEVAAG
                     EVLGLAALEGQGQDRLFEILSGNARAAGGEILVDGQPLRARHPYDAIRRGVVLVPSNR
                     LMALLPQRPIRENIAAPLYNRVLRWGPINSGRERRQVQEAVTRLSIDTRAAAQARRLS
                     GGNQQKLTIGRWLAAGFRTLLLFDPT"
BASE COUNT          388 a          933 c          874 g          327 t
ORIGIN      
        1 ggcgtggccg tcgtcaacga gatcaaggtc cggaccgcgc ggctgctggt cgcccgcggc
       61 ggcatgcctc cggtgttgac cagcggctcg gtggtgggta acgaggagtc cgcgcgcctg
      121 ttcgacgcgg cctacgacga gcacgcgcgc cgcctggcgc gggtgctcgc aggaagggag
      181 gagcacgtat gaggcgtccc cggtggatgc gggccggcat cctgatcgcc gccatggccc
      241 tggccgtgac ggcctgcggc ggcggtgacg acgacgcggc ctctggtggg agcggagccg
      301 ggtcgggtca gagcggcaac ttcgtcatcg gcgtcagcaa caccctggcc ggcaacggct
      361 ggcgcgagca gatgatctgc tcggtcaagg cgcaggccct ggccagcggc aaggtcagca
      421 aggtcatcgc catctccaag aacggcggcc cgaacgagca gatccaggac ctccagaacc
      481 tgatctccca gggcgccgac gccatcatcg tcaacccgtc cgaccgggag aagctcaacc
      541 cggtgatcca gcaggccacc cagaagaaga tcgtggtcgt ggccgtcgac caggccgtgt
      601 ccgagccgac ggcctacgtc gccaccaacg accaggtcgc ctacggccgc ctgggcgccg
      661 agtggctggc cgagcagctc ggcggcaagg gcaacgtcct ctacatgcgc gggatcgacg
      721 gcgtgccggc cgacgccgac cgcgacaagg gcttccagga ggtcatggcc cagaacccgg
      781 acatcaagat caaggaggtc ttcaccggct gggacttcac caagggcggg gacatcgccg
      841 tccaggagct cggctcgggg aactacgacg gcgtctggac ctcgggcatc gactacaccg
      901 tggtcaacgc cttcaagacc gtcggcaagg agccggtccc ggtcgtcggg gccgacaaca
      961 acgagttcat ccagcagctg ctcgacggct cgcccggcgc cgccgtcacc aacccggccg
     1021 tcatcggcgg ggtggggacg gccatcgccc ttgacgtgct ctcgggcaag acggtcaagc
     1081 gcgagaccct gctgactccc gaggtctggg acgccgagca ggacaaggcg accctggagg
     1141 ccaacagctt ccccgaccgc gaggccacct tcagctcggc ggtctcggtg aagccgttca
     1201 ccacctacac caacgagcag ctgttcgcct gcaagggccc gggcgagtag ccggcacctg
     1261 acccgctgat gtcacaacca ctcctgcagg cgaccgcggt ggccaaggcc ttcggtcccg
     1321 tggtcgccct gcgcgaggtc gacctgtcgg tgtccccggg cgaggtgcac gccttgctcg
     1381 gggccaacgg cgccggcaag tcgaccctgg tcaagatcct caccggcgtg ctccaccacg
     1441 actcgggcac ggtggccgtc aacggcgccc aggtcgagct gcgcagcccc accgaggccc
     1501 gcgggcgggg cctggccccg gtctaccagg acccggccat gatcggcgac ctcaccgtct
     1561 cccagaacct ccggctcacc ggggccgacc cggccaaggt ccgccacgag ctggccgaga
     1621 tggacctgga cggcctcgac ctggacgagc aggtccgcga catccccctg cccttcctgc
     1681 ggatgctcga cctggcccgg gcgctcgcct tcgatcccca gctgctggtg ctcgacgaga
     1741 tcacggcggc gctgccgccc gacctctccg accgggtgtt cgccatcatg acgcgctgga
     1801 aggagcgcaa ccggtcggtg ctgttcatct cccaccggct ggccgaggtc cgccagcact
     1861 gcgacatgtg caccgtcctg cgcgacggcc gcgacgtcgc ctccttccac cccggcagcg
     1921 agggcggcga gtcccagatc gtcgccgcca tgctcgccga ggccggcgcc ggggtccgcg
     1981 acaccgcccg ggagcgggcc gcccgggccg agggggagcg cacccagcgg ctggtcgtgc
     2041 gcgacctcct gatcgaccgg caggacgccg gcgtctcgtt cgaggtcgcc gccggcgagg
     2101 tcctggggct ggccgccctc gagggccagg gccaggaccg gctgttcgag atcctctccg
     2161 ggaacgcccg ggccgccggc ggcgagatcc tcgtcgacgg ccagccgctg cgggcccggc
     2221 acccctatga cgccatccgc cgcggggtgg tgctggtccc aagcaaccgg ctgatggccc
     2281 tgctgcccca gcggcccatc cgcgagaaca tcgccgcccc cctgtacaac cgggtcctcc
     2341 gctggggccc gatcaactcc ggaagggagc ggcgccaggt ccaggaggcg gtcacccgcc
     2401 tgtcgatcga cacccgcgcc gcggcccagg cgcggcgcct gtcgggcggg aaccagcaga
     2461 agctgaccat cggccgctgg ctggcggccg ggttccgcac cctgctgctg ttcgacccga
     2521 cg
//
LOCUS       DASSWR010000738         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_932108,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000738 DASSWR010000000
VERSION     DASSWR010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932108"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFO47_12400"
     CDS             complement(<1..510)
                     /locus_tag="VFO47_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789536.1"
                     /translation="MYPNLTPFLVGLGGAALLVAMAMAARGPLTRRLALRQLNRRRAE
                     AALVVAGSVLGTAIIVGSLVVGDTLDHSFKQNAYRYLGVVDEVVSSPDPAQGEAAARR
                     LEPLRGDPAIDGLLTVRRHGAAVTNGGKGEPQATVLELDFAAAARFEGPGSPLAGPGP
                     GPGRVVLSDA"
     gene            complement(513..1238)
                     /locus_tag="VFO47_12405"
     CDS             complement(513..1238)
                     /locus_tag="VFO47_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8789537.1"
                     /translation="MNENGQAEPLLVAERVRKVYRTGGIEVEALAGLDLEVRRGELVA
                     VMGPSGSGKTTLLNCLSGLDDIDGGRVLVEGQDLFAMTDARRTSHRARAMGFIFQAFN
                     LIPVFSAAENVELPLLLNGVPARRARDRAVAMLDRVGLGHRTGHRPNQLSGGEQQRVT
                     IARALAARPAIVWADEPTGNLDTAMADQVVALLHELNRQEGQTIVLVTHDPAIGGSAG
                     RVVRMRDGHLIGDERRPLAALRS"
     gene            complement(1374..2885)
                     /locus_tag="VFO47_12410"
     CDS             complement(1374..2885)
                     /locus_tag="VFO47_12410"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET8789538.1"
                     /translation="MIAYHRSPALFAYVLTVSAVGLILVALLVLGDGPATVLQQPPLF
                     WVLLTSMLVMESLPILSNVAGGTKYVTTVEAFAFAVLLSLGTTAAVVALVLVVLISEM
                     LRRAAFEKLVFNVGQYGLLMVVAGSVYELLGGDHPFAVQDLPAFVVAACVFFVGNLVL
                     VGVVTTLAHGRHFVADLKECIRAEAWPYTMVFAMAPIMLVVAEQSMALLPLVLLPMFA
                     VRDALRTAEEAEERRVVAEQAAEEARAVAAEKARLVEAQQALVEQLRESDRLKDDLLA
                     AVSHELRTPLAGMLGALATLSAREGQLTDPQRQELVDMAVRQGDRLKLQIEQLLLAAR
                     LERSGHEPAERPVVDAAAAARDAVAVARAAYPARQLVLEAREVLPVRALPEAILQVLG
                     NLLDNAAKHAPDKAPIRVEARRVGAQAVLSVTDTGPGVPPADRERIFERFTQLDSGAT
                     RRAGGVGLGLYIARQLANAQGGELLLTDPVPPGPGARFELRLPLAELAFRRPG"
BASE COUNT          426 a         1134 c         1204 g          374 t
ORIGIN      
        1 ggcgtcgctc aggaccaccc ggccaggccc ggggccgggc ccggccaggg gcgaccccgg
       61 gccctcgaag cgggccgcgg ccgcgaagtc gagctcgagc acggtggcct ggggctcgcc
      121 cttgccgccg ttggtcacgg cggcgccgtg gcgccggacg gtgaggaggc cgtcgatcgc
      181 ggggtcgccg cgcaggggct cgagccggcg ggccgccgcc tcgccctggg ccgggtcggg
      241 cgagctgacc acctcgtcga ccacgcccag gtaacggtag gcgttctgct tgaaggagtg
      301 gtcgagggtg tcgccgacga ccaggctgcc gacgatgatg gccgtgccca gcaccgagcc
      361 ggccacgacc agggcggcct cggcccggcg gcggttgagc tggcgcaggg ccaggcggcg
      421 ggtcaggggg ccgcgggcgg ccatggccat ggccaccagc agggcggccc cgcccaggcc
      481 gaccaggaac ggggtcaggt tggggtacat ggtcagctcc tcagcgcggc caggggacgc
      541 cgctcgtcgc cgatcaggtg gccgtcgcgc atgcgcacga cccggccggc cgagccgccg
      601 atggccgggt cgtgggtgac cagcacgatc gtctggccct cctggcggtt gagctcgtgc
      661 agcagggcga cgacctggtc ggccatggcg gtgtcgaggt tgccggtggg ctcgtcggcc
      721 cagacgatcg ccggccgggc ggccagggcg cgggcgatgg tgacccgctg ctgctcgccg
      781 cccgacagct ggttgggccg gtggccggtg cggtggccga gcccgacccg gtccagcatg
      841 gccaccgccc tgtcccgggc ccgccgggcc gggacgccgt tgaggagcag gggcagctcg
      901 acgttctcgg ccgcgctgaa caccgggatg aggttgaagg cctggaagat gaagcccatg
      961 gcccgggccc ggtggctggt gcggcgggcg tcggtcatgg cgaacaggtc ctggccctcg
     1021 accagcaccc ggccgccgtc gatgtcgtcc agccccgaca ggcagttcag cagcgtcgtc
     1081 ttgcccgagc cgctcgggcc catcacggcg accagctcgc cgcgccgcac ctccaggtcg
     1141 aggccggcga gggcctcgac ctcgatgcca ccggtccggt acaccttgcg cacccgctcg
     1201 gcgacgagca ggggctcggc ctggccgttc tcgttcacgt ccgctcctcg ggctcggggt
     1261 cgtctcgaga agcaagcctg gggcccggcc gccgccgcga ccatccggga tgcccccgga
     1321 agatccccca gccagccccc gccgggctca ggggtttccc cgggggggcc ggctcagccg
     1381 ggacgccgga aggccagctc ggcaaggggg aggcgcagct cgaagcgggc ccccgggccg
     1441 ggcgggaccg ggtcggtgag cagcagctcc ccgccctggg cgttggccag ctggcgggcg
     1501 atgtagagcc ccaggccgac cccgccggcc cggcgggtgg cgccggagtc gagctgggtg
     1561 aagcgctcga agatgcgctc gcggtcggcc ggcggtaccc cgggcccggt gtcggtgacc
     1621 gacagcacgg cctgggcccc gacccgccgc gcctcgaccc ggatcggcgc cttgtcgggg
     1681 gcgtgcttgg ccgcgttgtc gagcaggttg cccagcacct gcaggatcgc ctcgggcagg
     1741 gcccggacgg gcaggacctc ccgggcctcc agcaccagct ggcgggccgg gtaggcggcc
     1801 cgggccacgg ccacggcgtc gcgggccgcc gcggccgcgt cgaccaccgg ccgctcggcc
     1861 ggctcgtggc cgctgcgctc cagccgggcg gccagcagga gctgctcgat ctggagcttg
     1921 agccggtccc cctggcggac ggccatgtcg accagctcct ggcgctgggg gtcggtgagc
     1981 tggccctcgc gggcgctcag ggtggccagg gcccccagca tcccggccag gggggtgcgc
     2041 agctcgtggg agacggccgc cagcaggtcg tccttgagcc ggtcgctctc ccgcagctgc
     2101 tcgaccagcg cctgctgggc ctcgaccagc cgggccttct cggcggccac ggccctcgcc
     2161 tcctcggccg cctgctcggc gacgacccgc cgctcctcgg cctcctcggc ggtccggagg
     2221 gcgtcgcgca cggcgaacat cggcagcagc accagcggca gcagggccat gctctgctcg
     2281 gccaccacca gcatgatcgg ggccatggcg aagaccatcg tgtacggcca ggcctcggcc
     2341 cggatgcact ccttcaggtc ggcgacgaag tgccgcccgt gggcgagggt cgtgaccacg
     2401 ccgaccagca cgagattgcc gacgaagaag acgcaggcgg ccacgacgaa ggccgggagg
     2461 tcctggacgg cgaaggggtg gtcgccgccc aggagctcgt agacgctgcc ggcgacgacc
     2521 atgagcaggc cgtactggcc gacgttgaac accagcttct cgaacgcggc ccggcggagc
     2581 atttcggaga tgaggacgac caggaccagg gcgacgacgg ccgccgtcgt gcccagactg
     2641 agcaggacgg cgaacgcgaa cgcctcgacg gtcgtgacgt acttggtccc tccggcgacg
     2701 ttcgagagga tcggcaacga ctccatgacc agcatgctgg tcagcagcac ccagaacagc
     2761 ggtggctgct gcagcacggt cgccggcccg tcgccgagca ccagcagcgc gaccaggatg
     2821 agcccgaccg ccgagacggt cagcacgtag gcgaacagcg ctggtgagcg gtggtacgcg
     2881 atcatcgtga ccaaccagca agacgggaca gaccggcgag cggggcgcta gcacaccatg
     2941 tcggtcgatc cttgtcgagc gtttcagcgc tgccgccacg agcgggtcgc taggcccact
     3001 tcgagccggc gccgagggcg gcggccagcg cggccagcgc ggccagcgtc caggccgcac
     3061 gggccagacg gtacctaagg tgcgtcatgc ctcattcacc tcctccctga gcggcggggt
     3121 ggaggaggcg aacctcca
//
LOCUS       DASSWR010000739         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_847518,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000739 DASSWR010000000
VERSION     DASSWR010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847518"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>2733)
                     /locus_tag="VFO47_12415"
     CDS             complement(177..>2733)
                     /locus_tag="VFO47_12415"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET8789539.1"
                     /translation="ADPASDRPRFSMALPPPNVTGSLHIGHALDHTYQDYLARLHRMR
                     GFEVLWLPGMDHAGIATQNVVERELAKEGKTRFDLGREAFVERVWAWKQESGGRILGQ
                     MQRLGDSVDWSRERFTMDEGLSRAVREVFVSLYEQGLLYRGRRIINWCPRCLTALSDI
                     EVEHEEVPGELAHLRYPLADGSGWIGVATSRAETMLGDTAVAVHPDDPRYQGLIGRTV
                     CLPLVDRLIPVVADAAVDREFGTGAVKVTPAHDPNDHEIAERHGLPAVNILTEEAIVN
                     AEGGRFEGLDRYAARREVKAALAELGLLERVEEAPHSVGHCYRCRTEVEPRLSLQWFV
                     ATRPLADQAMEAVRSGQTRFEPVRYEKTFFGWMEQIRDWCVSRQLWWGHRIPAWYCPE
                     GHVTVARQDPAACGTCGAAELAQDEDVLDTWFSSGLWPFSTLGWPDQTEDLATFYPTS
                     VLVTGYDIIFFWVARMLMFGCHFQPPTPFEVVAIHGMVRDAQGKKMSKSFGNVIDPIE
                     LMDRYGTDALRFALIRGANPGSDVPMAEEWVEGARNFANKLWNVARFVLSTGGATSAP
                     PDPPEPRPSLGLADRWLLSRLERTRAAVTAAYDGFDPAEAARLLHGFAWSELADWAVE
                     LAKPRLAAGGEDARQAAATLTYALDVTLRLLHPVMPFVTEELGRALTGVETLTLGPWP
                     AERPGDLDPGAETGMADLQEAIVALRRFRAEHRVPTASRPRLVVVPADEGQAGLFRAE
                     ADSLRRLARLDAVEVGSAAPATPTAKLLAGRAELHLPLEGLLDLDEERARLDRELATL
                     GAERARAEAKLANPAFLEKAPPPVVAKARERLAEVDEALAKVRAQQVELSGAR"
BASE COUNT          355 a          972 c         1045 g          361 t
ORIGIN      
        1 cgtggccgac gtgcagcgac ccgctgacgg tcaggggcgg gaagtcaatc gcgtagacgt
       61 cctcccgggg tcgccgccgg tcgaaccggt agacgccgtc ggcgtcccag cggggtcccc
      121 acttggcctc gagcccgtcg agggagggcc gctccggaac ccgcatgggg cctccctcag
      181 cgggcgccgg acagctcgac ctgctgggcg cggaccttgg ccagggcctc gtcgacctcg
      241 gccaggcgct cccgggcctt ggccaccacc ggcggcgggg ccttctccag gaaggcgggg
      301 ttggccagct tggcctcggc ccgggcccgc tcggccccga gggtggccag ctcgcggtcg
      361 agccgggccc gctcctcgtc caggtcgagc agcccctcca gggggaggtg gagctcggcc
      421 cggccggcca gcagcttggc ggtgggggtg gccggggccg ccgagccgac ctcgacggcg
      481 tccaggcggg ccaggcggcg gaggctgtcg gcctcggccc ggaacaggcc ggcctggccc
      541 tcgtcggccg gcacgaccac cagccggggc cggctggccg tggggacgcg gtgctcggcc
      601 cggaaccggc gcagggccac gatcgcctcc tggaggtcgg ccatgccggt ctcggccccg
      661 gggtcgaggt cgccggggcg ttcggccggc cagggaccca gggtgagggt ctcgaccccg
      721 gtcagggccc ggcccagctc ctcggtgacg aacggcatca ccgggtgcag cagccgcagg
      781 gtgacgtcca gggcgtaggt cagggtggcg gcggcctgcc gggcgtcctc gccgccggcg
      841 gccagccgcg gcttggccag ctcgaccgcc cagtcggcca gctcgctcca ggcgaacccg
      901 tgcagcagcc gggccgcctc ggccgggtcg aagccgtcgt aggcggcggt cacggccgcc
      961 cgggtgcgtt ccaggcgtga gagcagccag cggtcggcca ggccgaggga cggcctcggt
     1021 tcgggggggt ccgggggggc gcttgttgcc cccccggtgg aaaggacgaa gcgagccaca
     1081 ttccagagct tgttcgcaaa gttgcgtgcg ccttccaccc actcctcggc catggggacg
     1141 tcgctgcccg ggttggcccc ccggatcagg gcgaaccgca gggcgtcggt gccgtagcgg
     1201 tccatcagct cgatcgggtc gatgacgttc ccgaacgact tggacatctt cttgccctgg
     1261 gcgtcgcgga ccatcccgtg gatggccacg acctcgaacg gggtcggcgg ctggaagtgg
     1321 cagccgaaca tcagcattcg ggccacccag aagaagatga tgtcgtagcc ggtgaccagc
     1381 accgaggtcg ggtagaaggt ggccaggtcc tcggtctggt cgggccagcc gagggtcgag
     1441 aacggccaca gcccgctcga gaaccaggtg tcgagcacgt cctcgtcctg ggccagctcg
     1501 gcggcgccgc aggtgccgca ggcggccggg tcctggcggg ccacggtgac gtgcccctcc
     1561 gggcagtacc aggcggggat gcggtggccc caccacagct ggcgggacac gcaccagtcg
     1621 cggatctgct ccatccagcc gaagaaggtc ttctcgtagc ggaccggctc gaaccgggtc
     1681 tgtccggagc ggaccgcctc catggcctgg tcggccagcg gcctggtggc cacgaaccac
     1741 tggagggaca ggcggggctc gacctcggtc cggcagcggt agcagtggcc gaccgagtgc
     1801 ggcgcctcct cgacccgctc cagcaggccc agctcggcca gggcggcctt gacctcgcgg
     1861 cgggcggcgt agcggtccag gccctcgaac cggccgccct cggcgttgac gatcgcctcc
     1921 tcggtgagga tgttgacggc cggcaggccg tggcgctcgg cgatctcgtg gtcgttgggg
     1981 tcgtgggccg gggtgacctt gaccgcgccg gtgccgaact cgcggtccac ggccgcgtcg
     2041 gcgaccaccg ggatcagccg gtcgaccagg ggcaggcaca ccgtcctgcc gatgaggccc
     2101 tggtagcggg ggtcgtcggg gtggacggcg accgcggtgt cgcccagcat cgtctcggcc
     2161 cgcgaggtcg ccaccccgat ccagcccgac ccgtcggcca gcgggtagcg caggtgggcg
     2221 agctcgcccg ggacctcctc gtgctcgacc tcgatgtccg acagggcggt caggcagcgg
     2281 gggcaccagt tgatgatccg ccggccccgg tagagcaggc cctgctcgta gagggagacg
     2341 aacacctcac ggacggcccg ggacaggccc tcgtccatgg tgaagcgctc ccgcgaccag
     2401 tcgaccgagt cgcccagccg ctgcatctgg ccgaggatgc gccccccgga ctcctgcttc
     2461 caggcccaga cccgctccac gaaggcctcc cggccaaggt cgaagcgggt cttgccctcc
     2521 ttggcgagct cccgctcgac cacgttctgg gtggcgatgc cggcgtggtc catgcccggc
     2581 agccacagca cctcgaaccc gcgcatgcgg tgcagccggg ccaggtagtc ctggtaagtg
     2641 tggtccaggg cgtggccgat gtgcagcgac ccggtcacgt tcggtggggg cagggccatg
     2701 ctgaagcggg gccggtccga ggccgggtcg gcc
//
LOCUS       DASSWR010000740         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1214307, whole genome shotgun sequence.
ACCESSION   DASSWR010000740 DASSWR010000000
VERSION     DASSWR010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214307"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFO47_12420"
     CDS             complement(<1..529)
                     /locus_tag="VFO47_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET8789540.1"
                     /translation="MPELGESITEGTITRWLKQEGDQVEADEPLFEVSTDKVDTEVPS
                     PVAGVLKSIKVQADETVEVGAELAIIEQDGEGGGGGESEEPGEAEEESAGSGEAEEAA
                     EADEGSEEAGTEEEREETAAQADEGDGGGEQAEKEPAEQPKAEQPKAEASGDGKTTTV
                     TMPELGESITEGTITR"
     gene            complement(566..1972)
                     /gene="lpdA"
                     /locus_tag="VFO47_12425"
     CDS             complement(566..1972)
                     /gene="lpdA"
                     /locus_tag="VFO47_12425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET8789541.1"
                     /translation="MAENSYDVVVLGSGTGGYSTAIRAAGLGLTVALVEKEDRCGGTC
                     LNWGCIPTKALLHAAEVMDGVHEASERWGIKATVESIDYSATVANREDIVTKNVKGLE
                     GHLKKDGIAIVRGRGRVTGPRTVDVEGTGELTAGKALVLATGSQPKSIPGLEIDGERV
                     ITSDHALKLDYLPKSVIVLGAGAVGVEFASFWRSMGAEVTIVEVLPGLVPLEDADSQR
                     ELARAFKKRGITSMVDTKLEDAKVTDEQVTVTVKDAKGKASEVSAELLLVATGRGPVT
                     KDLGYEELGVKLDRGFVVPKRWETLETDVEGVYAVGDLLPPPSLALAHASFAEGMLIA
                     ETVAGQSSPPIDYAGIPRVTFSTPEVASVGLTEAQAKEQGHEVVVNKFPFNATAKGLI
                     HGQGGMVKVVAAKDGPVLGIHLVGARVTDLVAEAMLIYNWEALPIDVAQYIHPHPTLS
                     EALGEAHLTLAGRPFHQL"
     gene            2102..2566
                     /locus_tag="VFO47_12430"
     CDS             2102..2566
                     /locus_tag="VFO47_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8789542.1"
                     /translation="MEVSEVMTKEVVTVGPHYNVADVASLMDAKGIGSVIVLEEERVM
                     GILTERDILKVIGAGEDPKNVAAHEALIGDLYTIAPDASVEEAASQMTQAKIRHLPVI
                     ADDQIVGIVSIRDVVRWSVREMAQATELPHLDVSRQVLSIVHPVDTAAGNPS"
     gene            2620..2931
                     /locus_tag="VFO47_12435"
     CDS             2620..2931
                     /locus_tag="VFO47_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789543.1"
                     /translation="MDREATSEDLDALREFARTRVGVEFFVEPETMVTDTTAVAVAAD
                     GEWTRRRVGSPQVIRQVAKQLQLPVYDVQIVGYPERMRAYNARLKARRSDRLLGKEDP
                     S"
     gene            2928..>3597
                     /locus_tag="VFO47_12440"
     CDS             2928..>3597
                     /locus_tag="VFO47_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HET8789544.1"
                     /translation="MTADGELHRSPLDAVHRRLEAKMGAFAGWDMPIQYQGTMSEHKA
                     VRESVGVFDLSHLGKVEVRGPGAHDALQHALSNDLDRLAEPGAAQYTLCLTDEGTIVD
                     DLIVYHEADGYLLVPNAANWPKVADAVRASDPGRTEVTPRPDVAVLAVQGPRAPQLVG
                     GLFPQAADLPYMHMAVTEYRGAPARVCRTGYTGERGFELLVPGEVAPALWEELFERGA
                     PLEVV"
BASE COUNT          474 a         1372 c         1200 g          551 t
ORIGIN      
        1 agcgggtgat ggtgccttcg gtgatcgact cgcccagctc gggcatggtc accgtggtcg
       61 tcttgccgtc ccccgacgcc tccgccttgg gctgctccgc cttgggctgc tcggcgggct
      121 ccttctcggc ctgctccccg ccgccgtcgc cctcgtcggc ctgggccgcc gtctcctccc
      181 gctcctcctc ggtgccggcc tcctcgctgc cctcgtccgc ctcggcggcc tcctcggcct
      241 ccccggaccc ggccgactcc tcctcggcct cccccggctc ctcggactcg ccgccgcccc
      301 cgccctcgcc gtcctgctcg atgatggcca gctcggcccc gacctcgacc gtctcgtcgg
      361 cctggacctt gatcgacttc aggaccccgg cgacggggga ggggacctcg gtgtcgacct
      421 tgtcggtgga cacctcgaac aggggctcgt cggcctcgac ctggtcgccc tcctgcttga
      481 gccagcgggt gatggtgccc tcggtgatcg actcaccgag ctccggcatc ttgactggtg
      541 tcgccattga ccatcgtctc ccggctcaga gctggtggaa cggccgtccg gcgagggtca
      601 ggtgcgcctc gccgagcgcc tcggacaggg tcgggtgcgg gtggatgtac tgggcgacgt
      661 cgatcggcag cgcctcccag ttgtagatca gcatcgcctc ggcgaccagg tcggtgaccc
      721 gggcgccgac caggtggatc ccgagcacgg gaccgtcctt ggccgcgacc accttgacca
      781 tgcccccctg gccgtggatc aggcccttgg ccgtggcgtt gaaggggaac ttgttgacaa
      841 ccacctcgtg tccctgctcc ttggcctggg cctcggtgag gccgaccgac gccacctccg
      901 gcgtggagaa tgtgacccgc gggatgccgg cgtaatcgat cggcggcgag gactggccgg
      961 cgaccgtctc ggcgatcagc atcccctcgg cgaacgaggc gtgggccagg gccagcgacg
     1021 gcgggggcag caggtcgccg accgcgtaca cgccctcgac gtcggtctcc agggtctccc
     1081 agcgcttggg gacgacgaag ccccggtcca gcttgacccc gagctcctcg tagcccaggt
     1141 ccttggtgac cgggccgcgg ccggtggcca ccagcagcag ctcggccgac acctccgagg
     1201 ccttgccctt ggcgtccttg accgtcaccg tgacctgctc gtcggtgacc ttggcgtcct
     1261 ccagcttggt gtcgaccatc gaggtgatgc cgcgcttctt gaacgcccgg gccagctcgc
     1321 gctggctgtc ggcgtcctcc agcgggacca ggccgggcag gacctcgacg atcgtcacct
     1381 cggcgcccat ggagcgccag aacgaggcga actcgacccc gaccgccccg gcgccgagca
     1441 cgatcaccga cttgggcagg tagtcgagct tcagcgcgtg gtcggaggtg atcacccgct
     1501 cgccgtcgat ctccaggccg gggatcgact tgggctggga cccggtggcc aggaccaggg
     1561 ccttgcccgc ggtcagctcc ccggtcccct cgacgtcgac ggtccgcggc ccggtgaccc
     1621 gcccgcgccc gcggacgatc gcgatgccgt ccttcttcag gtgcccttcg agccccttga
     1681 cgttcttggt gacgatgtcc tcgcggttgg ccaccgtggc cgagtagtcg atcgactcga
     1741 ccgtggcctt gatgccccag cgctcggacg cctcgtggac gccgtccatc acctcggcgg
     1801 cgtgcagcag cgccttggtc gggatgcagc cccagttgag gcaggtcccg ccgcagcggt
     1861 cctccttctc caccagggcg accgtgaggc ccaggccggc cgcccggatc gcggtcgagt
     1921 acccgccggt gccgctcccc aggactacga catcgtagga gttctccgcc atggccgtcg
     1981 gctcctttag ggtagggctt ccccggctga gtctagcgag gagtaggctc agaggcagag
     2041 gccggggcgc tgatggcggc ccggccggac cctctggcgt cgctgaaagg aggaccgcct
     2101 cgtggaggtc tcggaggtca tgaccaagga ggtcgtcacc gtcggcccgc actacaacgt
     2161 cgccgacgtg gccagcctca tggacgccaa gggcatcggc tcggtgatcg tcctcgagga
     2221 ggagcgcgtc atgggcatcc tcaccgagcg cgacatcctc aaggtcatcg gggccggcga
     2281 ggaccccaag aacgtggccg cccacgaggc cctcatcggc gacctgtaca cgatcgcccc
     2341 ggacgcctcg gtcgaggagg cggccagcca gatgacccag gccaagatcc ggcacctgcc
     2401 ggtgatcgcc gacgaccaga tcgtcggcat cgtctcgatc cgcgacgtgg tccgctggag
     2461 cgtccgcgag atggcccagg cgaccgagct gccccatctg gacgtgtcac gtcaggtcct
     2521 gtcgatcgtc cacccggtcg acaccgccgc cggcaacccc agttgagctt cctcgacaac
     2581 ctccgcatcc ggcgcaaccg cggccggagg gtcgcctcca tggaccggga ggcgacctcc
     2641 gaggatctgg acgccctgcg cgagttcgcc cgcacccggg tcggggtgga gttcttcgtc
     2701 gagccggaga ccatggtcac cgacaccacc gcggtcgcgg tggccgccga cggcgaatgg
     2761 acccgccgcc gggtcggctc cccgcaggtc atccggcagg tggccaagca gctccagctg
     2821 cccgtctacg acgtgcagat cgtcggctac ccggagcgga tgcgcgccta caacgcccgc
     2881 ctcaaggcca ggcgctccga ccgcctcctc ggcaaggagg acccctcgtg accgctgacg
     2941 gcgagctcca ccgctccccc ctggacgccg tccaccggcg cctcgaggcc aagatgggcg
     3001 ccttcgccgg ctgggacatg ccgatccagt accagggcac gatgtccgag cacaaggcgg
     3061 tgcgcgagtc ggtgggcgtg ttcgacctct cccacctggg caaggtcgag gtccgcggcc
     3121 cgggcgccca cgacgccctc cagcacgccc tctccaacga cctggaccgc ctggccgagc
     3181 cgggcgccgc ccagtacacc ctctgcctca ccgacgaggg cacgatcgtc gacgacctga
     3241 tcgtctacca cgaggccgac ggctacctgc tcgtccccaa cgccgccaac tggcccaagg
     3301 tggccgacgc cgtccgcgcc tccgaccccg gccgcaccga ggtcaccccc cgccccgacg
     3361 tcgccgtgct cgccgtccag gggccgcgcg ccccccagct cgtcgggggc ctgttccccc
     3421 aggccgccga cctgccctac atgcacatgg ccgtcaccga ataccggggc gccccggcgc
     3481 gggtgtgccg caccggctac accggcgagc gcgggttcga gctgctggtc ccgggcgagg
     3541 tcgcgccggc gctgtgggag gagctgttcg agcggggggc gccgctcgag gtcgtcc
//
LOCUS       DASSWR010000741         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_733989,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000741 DASSWR010000000
VERSION     DASSWR010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733989"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VFO47_12445"
     CDS             complement(<1..1391)
                     /locus_tag="VFO47_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET8789545.1"
                     /translation="MAVATSPPRPASRATPSLRGVVLALFVASGAAGLIYQVVWSREL
                     VLVFGNTTQAVATIVTAFMAGLGFGSLAGGRLADTSARPLRLYGLIELAVAAMAALLP
                     FAFSSLAELYRGVWPSLVERPGQLAGVRFGLALAAVAPATFLMGMTLPLLTRYLVRTL
                     DEAGARLGELYAANTVGAMAGTLLAGFVLIELFGLRLTSYIAVALNLVAGVGALLLSR
                     RWETGPDAAGGPAARRERPARPEVPRAFRPRRRAVLAATFVSGFVSLALEVLWTRMLA
                     EGTGSSIYIFTTILAIFLLGIALGSFLYRRFSRPGGERLGTLGLCLAGVGVLAQATVV
                     LGSGMVGHVPFVVRTVVVLLPATILMGYAFPLAGRLATPSAEAAGGSVGLLYAANTGG
                     SILGSFSAAFVLAGTLGTNGSILLLGALNLLAGAALFLADPVGRPGPAGRPASAGATS
                     SGPGPAGPAYRGRA"
     gene            1602..2534
                     /locus_tag="VFO47_12450"
     CDS             1602..2534
                     /locus_tag="VFO47_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET8789546.1"
                     /translation="MALVAMVVVTGCDQGADTAGGGGDEAAATQSADQAAGGAPGQAA
                     GGAADQAAPAAVATEARESDKGGSTGSTGAAAQVRLVDLGNRVVRTASVDLEVGEGDL
                     NKTINDATDVVLQAKGLYVGSSTSVPEGEPASGQVTFRVPVDAFEPVLRQLKELGTYR
                     GEKSSTEDVTNQYVDLNGQLAAWRAQERVYLRLLDRAKSVTDVIAVQNQLQQVQSNIE
                     RLQGQLNHLEDQSSFSTIVLQLTEPGAGGPAPQPTGRLARAWATAVNGLGVMAAAVLV
                     AVVWLTPVLVLAGLVLFGVRALRRPRPVSGKVSG"
     gene            complement(2635..3135)
                     /locus_tag="VFO47_12455"
     CDS             complement(2635..3135)
                     /locus_tag="VFO47_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET8789547.1"
                     /translation="MVAVLAAALGVAGQTVLAPPAQARAINVSDAQQHRIRKAVNRYA
                     EIQVAQREPYCYGGTGPNCFDCSGLVVAAYRYAGVNLTGRGVRTSRQLYSFTTRTSLS
                     TSRTGDLLFYDYDHNGVVSHVEIVYRKNGSGPRMAVSATHSGGPPVNFHKIRTTGLVK
                     VGRVVT"
BASE COUNT          535 a         1170 c         1373 g          404 t
ORIGIN      
        1 gcccgccccc ggtacgccgg cccggcggga cccgggcccg aggaggtcgc gccggcagag
       61 gccggtcgcc cggctggtcc cgggcggccc accgggtcgg cgaggaagag ggccgcgccg
      121 gcgaggaggt tgagggcgcc caggagcagg atggaaccgt tggtgccgag ggtcccggcg
      181 aggacgaagg cggcgctgaa cgagccgagg atggagccgc cggtgttggc cgcgtacagc
      241 aggccgacgc tgcccccggc cgcctccgcc gacggggtgg ccaggcggcc ggcgagcggg
      301 aaggcgtagc ccatcaggat cgtcgccggc agcagcacca ccacggtgcg gaccacgaac
      361 gggacgtggc cgaccatccc gctgccgagc acgaccgttg cctgggcgag cacgccgacc
      421 ccggccaggc agaggccgag cgtgcccagc cgctcgcccc cgggccggga gaagcgccgg
      481 tacaggaagc tcccgagggc gatgccgagc aggaagatgg ccaggatggt ggtgaagatg
      541 tagatgctgg agccggtgcc ctcggccagc atccgggtcc acagcacctc cagggcgagg
      601 gacacgaacc cggagacgaa cgtcgccgcc agcaccgccc gccggcgggg ccggaaggcg
      661 cgcggcacct ccggccgggc cggacgctcg cgccgggcgg ccggcccacc ggccgcgtcg
      721 ggacccgtct cccagcgccg cgacagcagc agggcgccaa ccccggccac gaggttgagg
      781 gcgacggcga tgtagctggt gaggcgcagg ccgaacagct cgatgagcac gaagccggcc
      841 agcagcgtcc cggccatggc gccgacggtg ttggccgcat acagctcgcc caggcgggcg
      901 ccggcctcgt ccagggtgcg gaccaggtag cgggtgagca ggggcagggt catccccatc
      961 aggaacgtgg ccggcgccac cgccgcgagg gcgaggccga agcggacgcc ggccaactgg
     1021 cccggccgct cgaccaggct cggccacacg ccccggtaga gctcggccag gctgctgaag
     1081 gcgaacggca gcagggcggc catggcggcg acggccagct cgatcagccc gtagaggcgc
     1141 agcggccgcg cgctggtgtc ggccagccgg cccccggcca ggctgccgaa gccgaggccg
     1201 gccatgaacg cggtcacgat cgtcgccacc gcctgggtgg tgttgccgaa caccagcacc
     1261 agctcgcgcg accacaccac ctggtagatg agcccggcgg cgcccgaggc gacgaacagg
     1321 gcgagcacca cgccgcgaag cgacggggtg gcgcggctgg cgggtcgggg tggggaggtg
     1381 gcgacggcca cgggcaggac tcctcggcga tcgatgcaga cccggaggag gcttccagat
     1441 ggcggcccga cccggcaagc ggtcgtccac agccctccgg gtcgagcggg cccgcggggc
     1501 cgggtcggag ggccaggaac cgttcgggtc cggacggcgt cccaaccagc agacaccgac
     1561 atgggagggt gaggatgcag cttcgccgga cggcggttgg ggtggcgctg gtcgcgatgg
     1621 tggtcgtgac cggctgcgac cagggggcgg acaccgccgg cggaggcggc gacgaggccg
     1681 cggcgacgca gtcggccgac caggcagcgg gcggggcacc gggccaggcg gcgggcggcg
     1741 cggccgatca ggcggccccg gccgccgtgg ccacggaggc cagggagagc gacaagggcg
     1801 ggagcaccgg ctcgaccggg gcggccgccc aggtgcggct ggtcgacctc ggcaaccggg
     1861 tcgtccgcac ggccagcgtc gacctggagg tcggcgaggg cgacctcaac aagaccatca
     1921 acgacgccac cgacgtcgtg ctccaggcca agggcctgta cgtcgggtcg tccacctcgg
     1981 tgccggaggg tgagccggcc agcggccagg tcaccttccg ggtcccggtg gacgcgttcg
     2041 agccggtgct gcgccagctc aaggagctgg gcacctaccg cggggagaag tcgagcaccg
     2101 aggacgtgac caaccagtac gtcgacctca acggccagct ggccgcctgg cgggcccagg
     2161 agcgggtgta cctgcgcctg ctcgaccggg ccaagtcggt caccgacgtg atcgccgtgc
     2221 agaaccagct ccagcaggtg cagtcgaaca tcgagcggct ccaggggcag ctgaaccacc
     2281 tcgaggacca gtcgtcgttc tccaccatcg tgctgcagct caccgagccg ggcgccggcg
     2341 gcccggcccc ccagccgacc ggccggctgg cccgggcgtg ggcgaccgcc gtgaacgggc
     2401 tcggggtgat ggccgcggcc gtgctggtcg cggtggtctg gctgacgccg gtgctggtcc
     2461 tggccgggct ggtgctgttc ggggtccggg cgctccggcg gcccaggccg gtgtcgggga
     2521 aggtttcggg gtgatcatcc agatcacgtt gtacgggctc ttgagcggct tcaagaacct
     2581 ggcccgaggc tacctcgtga ggagctgcct cggcgtcccg ccggcccggc cgacctaggt
     2641 caccactctg cccaccttga ccagccccgt ggtgcggatc ttgtggaagt tcaccggggg
     2701 gccgcccgag tgggtcgctg agaccgccat cctcggcccg ctgccgttct tgcggtacac
     2761 gatctccacg tgggagacga ccccgttgtg gtcgtagtcg tagaacagca ggtccccggt
     2821 ccgtgaggtg gagagcgagg tccgggtcgt gaaggagtac agctggcggg aggtccggac
     2881 gccgcggccg gtcaggttga ccccggcgta gcggtaggcg gcgacgacca ggccggagca
     2941 gtcgaagcag ttgggtccgg tgccgccgta gcagtagggc tcacgctggg cgacctggat
     3001 ctcggcgtag cggttgaccg ccttgcggat ccggtgctgt tgggcgtccg acacgttgat
     3061 ggctcttgcc tgggccggcg gcgccaggac ggtctggccg gcgacgccca gcgcggccgc
     3121 gaggacagcg accacgagca acttgcagcg caaagggggg ctcctctccg gtaggggggt
     3181 tcggagggcg gggaggaggc taccccggtc agggcgctct tgtccaattc gctaggttat
     3241 gcagcatcga taggttctgc agccacgctg ggttctggat gctcaaggga ttcagcaggg
     3301 ttgctgggtt ctgcgagttg aacgctgcag tgagcttggg aggacctctc ggccttggcg
     3361 cggcagtcgg actggccgaa cccccaccac acccgccgac cagcagggca agcaggatca
     3421 ggggagcaag cctcggggtg gagcgggcga agggaatcga accctcaccg cgagcttggg
     3481 aa
//
LOCUS       DASSWR010000742         4098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_111738,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000742 DASSWR010000000
VERSION     DASSWR010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111738"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1119)
                     /locus_tag="VFO47_12460"
     CDS             complement(409..1119)
                     /locus_tag="VFO47_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET8789548.1"
                     /translation="MTRRRSQADEAALLQVLGQSPIFRHGRPDALDQLAKQATPFDYP
                     VGSFLMRQGEPADHVLVLTAGEVAIVSPVRGGGELVHTIIRAGNLIGELALLNEGRRT
                     AGARATSPTTAWAIGRDPFWGFLEANPPASSALLRQVAARLAAREALIDDLLSLDVKS
                     RLAKVLLGLADDLGEPDPDGGTRISLRLTHRDLAGMVGASRENVSRALGAFRKRGFID
                     YDSDSIRLRNPDALRRLT"
     gene            complement(1116..>4098)
                     /locus_tag="VFO47_12465"
     CDS             complement(1116..>4098)
                     /locus_tag="VFO47_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8789549.1"
                     /translation="LLNETFRRLAAEVRRFGGTLEKYIGDAIFAVFGFPTGHDDDAAR
                     AVRAALALRDIVKTAETALGDVPLHLRIGLDTGEVAAGPWSGDLRVTGPAVHTAARIQ
                     QAAEPDQILLSARTLRAARDIVEVGPAELLTVRGQRQPVEVAEVLGLAPAEPVTEAMV
                     GREADLPRLIGALDHAAHHNRLVLLVGDAGVGKSTLARAATAQIGDRVHVLWGRCLPD
                     WQSLPFWPVREVLAAAAGMAATDPAGVLASSIGRLVAETWPDPRAAPATAEALCRLAG
                     LDSDEPGPMSQPVLGDARELAAALAGVLCGLARNERVLVVLEDLHWATSDLLEVASTL
                     VTDGCRAEGQLAYLGISRPELPGLPAWLVRTGTQRIDLDPLGERPAAELLVSVLGADT
                     DPGLAGRVFEHSKGNPLFVKELALALREAERTGPDQPSLPIPDSLQALVAARLDRLPL
                     STKRVLCRAAVVGKWFSHAALAALAQPGDGELDADLDRLVRMGLIERLPERLAGGQER
                     FAFHHALFRDVAYSMLPKAGRSELHGRLAGWLAGAPGEEPSMPEVVARHLVQAVRLAG
                     EVRAPTAEDRELAARAVAACQRAARRLRDQEALAAAAQMLDDALALADLAETGVEERA
                     ELHLERGTVRGATGDLPGAVADLGPATVAERTAVRAQAWTELSRVHVLFGQFAESAAA
                     ADRAIVEAVEAGDPALIAQATRVKAYPPYLAGDLAGAGRLLDEAIVQARRAGQAKLVI
                     ELRASLLPMRLYLATPLDRLRAEALGLVEDARAAGRRSAEASAQVSLGEVAWLQGDPD
                     AAERHFAEGNRLSLEVGFTRKRLWSLLGLTLVGIARDEPEAARRRAQEAIELTTQPDG
                     SADVEAELHLAEACLAGADPDEAAAAVARAWAAVQDVDVFSRARLQRTEAKLALASGD
                     PARAVALLERSLAALETTGHRLDQLHTLVELAPALRRVGRDDDAGPVAKRALDQATTM
                     GAHALVRRLTQAEVPEGGVGR"
BASE COUNT          476 a         1545 c         1600 g          477 t
ORIGIN      
        1 gtgggcatcg ccgtcgccga cggcctggag gccgcgaccc gcgccgtgct ggacgggacc
       61 gtggcgccgg cgggcgggaa tgcgaccagg cccgctccgg ccgtgacgga gcgggcctgg
      121 cccgggatgc cggttggtcg cggacgaccg cgcccctgac cggcaccgcc agcggcgggt
      181 tcgggccccc ccggtcccgg tgaccgcccc cgtcggtccc ggaccccccc gaacaccgaa
      241 tccttccgct gtgtccccat ccccctgccg gttccggcgg gctcctcctg tgtctccgcc
      301 ggtacctctc gggaggacga gatgagcgta gccgccgccc cccgctggcg tcggtgctct
      361 gggtcaccgc atcccggtga tctccgacac aaccggcaca gaccaggctc aggtgagccg
      421 gcggagcgcg tcggggttgc gcaggcggat cgagtcggag tcgtagtcga tgaagccgcg
      481 cttgcggaac gcccccagag cccgactgac gttctcgcgg ctggccccga ccatgccggc
      541 caggtcgcgg tgggtgaggc gcagggagat cctggtcccg ccgtcggggt cgggctcgcc
      601 gaggtcgtcg gccaggccga gcaggacctt ggccagccgg ctcttgacgt cgagcgacag
      661 caggtcgtcg atcagcgcct cacgggcggc cagccgggcc gccacctgcc gcagcagggc
      721 cgaggaggcg ggcgggttgg cctccagaaa gccccagaag gggtcgcggc cgatggccca
      781 ggcggtggtg ggcgaggtcg cccgcgcccc ggcggtccgc cggccctcgt tcagcagggc
      841 gagctcgccg atcaggttgc cggcccggat gatcgtgtgg acgagctcgc cgccgccccg
      901 gacgggggag acgatcgcca cctcgccggc ggtcagcacc agcacgtggt cggcgggttc
      961 gccctggcgc atcaggaagc tgcccaccgg gtagtcgaac ggggtcgcct gcttggccag
     1021 ctggtcgagg gcgtcgggcc gcccgtggcg gaagatcggg ctctgcccga gcacctggag
     1081 caacgccgcc tcgtcggcct gggatcgccg tcgggtcatc tcccaactcc cccttccggg
     1141 acctctgcct gggtcaggcg gcggaccagg gcgtgcgcgc ccatggtggt cgcctggtcg
     1201 agcgctcgct tggccacggg gccggcatcg tcgtcgcggc cgacccggcg cagggccggc
     1261 gccagctcga ccagcgtgtg cagctggtcg aggcggtgac cggtcgtctc cagggcggcc
     1321 agggagcgct ccagcagcgc caccgccctc gccgggtcgc ccgacgccag agccagcttg
     1381 gcctcggtgc gctgcagccg ggcccgcgag aacacgtcga cgtcctggac cgccgcccag
     1441 gcgcgggcca cggccgcggc cgcctcgtcc gggtcggcgc cggccaggca ggcctcggcc
     1501 aggtgcagct cggcctcgac gtcggcgctg ccgtcgggct gggtggtgag ctcgatcgcc
     1561 tcctgggccc ggcggcgggc cgcctccggc tcgtcgcggg cgatcccgac cagtgtcagc
     1621 ccgagcagcg accacagccg tttgcgggtg aagccgacct cgaggctgag gcggttgccc
     1681 tcggcgaagt ggcgctcggc ggcgtcgggg tcgccctgca gccaggccac ctcgcccagg
     1741 gagacctggg cggacgcctc ggcgctgcgc cggccggccg cgcgggcatc ctcgaccagc
     1801 ccaagggcct cggcgcgcag ccggtccagc ggggtcgcca ggtacaggcg catcggcagc
     1861 aggctggccc gcagctcgat caccagcttg gcctggccgg cccggcgcgc ctggacgatc
     1921 gcctcgtcga gcaggcggcc cgccccggcc aggtcgccgg ccaggtacgg cgggtaggcc
     1981 ttgacccggg tcgcctgggc gatgagcgcg gggtcgccgg cctcgaccgc ctcgacgatg
     2041 gcccggtcgg cggccgccgc cgactcggcg aactgcccga acagcacgtg cacccgggag
     2101 agctcggtcc aggcctgggc ccgcaccgcc gtccgctcgg cgaccgtggc cggcccgagg
     2161 tcggccacgg cgcccggcag gtcgccggtg gccccgcgga ccgtcccccg ctccaggtgc
     2221 agctcggccc gttcctcgac accggtctcg gccaggtcgg ccagggccag ggcgtcgtcg
     2281 agcatctggg cggccgcggc cagggcctcc tggtcgcgca ggcgccgggc ggcccgctgg
     2341 caggcggcga cggcgcgggc ggccagctcc cggtcctcgg cggtcggggc ccgcacctcg
     2401 ccggccagcc ggaccgcctg gaccaggtgg cgggcgacca cctcgggcat gctcggctcc
     2461 tcgccgggcg ccccggccag ccacccggcc aaccggccgt ggagctcgga ccgcccggcc
     2521 ttgggcagca tgctgtaggc cacgtcacgg aacagggcgt ggtggaaggc gaagcgctcc
     2581 tggccgccgg ccaggcgctc gggcagccgt tcgatcaggc ccatccggac cagccggtcc
     2641 aggtcggcgt cgagctcgcc gtcgccgggc tgggccaggg cggccagggc ggcgtgcgag
     2701 aaccacttgc cgaccacggc ggcccggcac aggacgcgct tggtcgacag cggcagccgg
     2761 tcgagccggg ccgcgaccag cgcctggagg gagtcgggga tgggcaggga gggctggtcg
     2821 ggcccggtcc gttccgcctc ccggagggcg agggccagct ccttgacgaa cagcgggttg
     2881 cccttgctgt gctcgaagac ccggccggcc agccccgggt cggtgtcggc gcccagcacc
     2941 gagacgagca gctcggcggc gggccgctcg ccgagcgggt cgaggtcgat ccgctgggtc
     3001 cccgtcctga ccagccaggc cggcaggccg ggcagctcgg gccggctgat gcccaggtag
     3061 gcgagctgcc cctcggcccg gcagccgtcg gtgaccagcg tcgaggccac ctccagcagg
     3121 tcgctggtgg cccagtgcag gtcctccagg accaccagga cccgttcgtt cctggccagg
     3181 ccgcacagca ccccggccag ggcggcggcc agctcgcggg cgtcgccgag gacgggctgg
     3241 gacatcgggc cgggctcgtc cgagtcgagg ccagccagcc ggcacagggc ctccgcggtg
     3301 gccggggcgg cccgcgggtc cggccaggtc tcggcgacca gccggccgat cgaggaggcg
     3361 agcacgccgg ccgggtcggt ggcggccatc ccggcggccg cggccagcac ctcgcggacc
     3421 ggccagaagg gcaggctctg ccagtcgggc aggcagcgtc cccagaggac gtggacgcgg
     3481 tcgccgatct gggccgtggc cgcccgggcc aaggtcgact tgccaacgcc ggcgtcgccg
     3541 accagcagga ccagccggtt gtggtgggcg gcgtggtcga gggcgccgat cagccgtggc
     3601 aggtcggcct cgcggccgac catcgcctcg gtcacgggct cggcgggggc cagcccgagc
     3661 acctccgcca cctcgaccgg ctggcgctgg ccgcggaccg tcagcagctc ggccgggccg
     3721 acctcgacga tgtcgcgggc cgcgcgcagg gtccgggccg acaggaggat ctggtcgggc
     3781 tcggccgcct gctggatgcg ggccgcggtg tgcaccgccg ggccggtcac ccgcaggtcc
     3841 cccgaccacg gcccggccgc gacctcgccg gtgtcgagcc cgatgcgcag gtgcaggggg
     3901 acgtcgccga gggcggtctc ggccgtcttg acgatgtcgc gcagggccag cgccgcccgc
     3961 accgcccggg cggcgtcgtc gtcgtggccg gtcgggaacc cgaacacggc gaagatggcg
     4021 tcgccgatgt acttctccag ggtcccgccg aagcggcgga cctcggcggc gaggcgccgg
     4081 aaggtctcat tcagcagg
//
LOCUS       DASSWR010000743         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_734321,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000743 DASSWR010000000
VERSION     DASSWR010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734321"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..758
                     /locus_tag="VFO47_12470"
     CDS             114..758
                     /locus_tag="VFO47_12470"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET8789550.1"
                     /translation="MAIRRLGHEHGFAVDDTADPACIGQGLAGYRAVVFLSTTGDLLD
                     PGQQAALERYLQDGGGWVGVHGAADAEYDWPWYGGLVGAWFRRHPAVQRATVRPDPGA
                     AALAGKPPARWERGDEWYDFRANPRGRARVLATVDESTYRGGGMGTDHPVAWCQEYGG
                     GRAWYSAMGHTGGSFREPPFLAHLLAGIRYAAGRTAARCTPVPNRRRAGGGSGG"
     gene            complement(721..>2189)
                     /gene="pgi"
                     /locus_tag="VFO47_12475"
     CDS             complement(721..>2189)
                     /gene="pgi"
                     /locus_tag="VFO47_12475"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET8789551.1"
                     /translation="VTTDTLRMLVALAKRAGVEQLRDAMFAGEHINVTEDRAVLHTAL
                     RAPEGRQVRVDGHDVVPEVHEVLGRMRAFAEEVRSGRWLGHTGRPIRNVVNIGIGGSD
                     LGPAMAYEALLPYTDRSRRFRFVSTVDGADIAEATGDLDPAETLFVVSSKTFTTIETL
                     TNARTARAWLVDALGDEAAVSRHFVAVSTNAEKVAEFGIDPANMFGFWDWVGGRYSYP
                     SAIGLSLMVAIGPDHYDELLAGFHRMDEHFRTAPFECNLPVLLGLLGVWYINFFGAET
                     QAVLPYSQYLGRFPAYLQQLDMESNGKSVTLDGAPVGVDTGPVLWGQPGTNGQHAFYQ
                     LIHQGTRLVPCDFIGICRPAEVVGDHHDLLMANFLAQTEALAFGKTREEVEAEGVSPD
                     LAPHRTFPGNRPTSTILAPELSPSVLGQLTALYEHKVFTQGVIWDINSFDQWGVELGK
                     VLATRIAAELDPAAGDDLGHDSSTNTLIRRYRRLRDGG"
BASE COUNT          288 a          754 c          846 g          301 t
ORIGIN      
        1 ccccgagccc gcgccggcca cgcccatggc caccaccacc gccccgggcg ggttccgggt
       61 gctggtgtcc acccggacgg ccgggttccg gcacgcccca tgcggctggg gtggtggcca
      121 tccgccgcct gggacacgag cacgggttcg cggtcgacga caccgccgac ccggcctgca
      181 tcggccaggg cctggccggg taccgggcgg tggtgttcct gtcgaccacg ggggacctgc
      241 tcgaccctgg ccagcaggcc gccctggagc gctacctcca ggatgggggc ggctgggtcg
      301 gcgtgcacgg ggccgccgac gccgagtacg actggccctg gtacggcggg ctggtcggcg
      361 cctggttccg gcgccacccg gccgtccagc gggccaccgt tcgccccgac cccggcgccg
      421 ccgccctggc cgggaagccg ccggcgcgct gggaacgcgg cgacgagtgg tacgacttcc
      481 gggccaaccc gcgcggccgg gcccgggtgc tggccaccgt cgacgagtcg acctatcgcg
      541 gcggcggaat ggggaccgac catcccgtcg cctggtgcca ggagtacggc ggcggccggg
      601 cctggtacag cgccatgggc cacaccggcg ggagcttccg ggagccgccg ttcctggccc
      661 acctgttggc cggcatccgc tatgccgccg ggcggaccgc ggcccgctgc accccggttc
      721 ctaaccgccg tcgcgcaggc ggcggtagcg gcggatgagg gtgttggtgg agctgtcgtg
      781 gcccaggtcg tcgccggccg ccgggtcgag ctcggccgcg atccgggtgg ccaggacctt
      841 gcccagctcc acgccccact ggtcgaagct gttgatgtcc cagatcaccc cctgggtgaa
      901 caccttgtgc tcgtacaggg cggtgagctg gcccagcacc gacgggctga gctcgggggc
      961 gaggatggtg ctggtgggcc ggttgccggg gaaggtgcgg tggggggcga ggtcggggct
     1021 gacgccctcg gcctcgacct cctcgcgggt cttgccgaag gccagggcct cggtctgggc
     1081 gaggaagttg gccatgagca ggtcgtggtg gtcgcccacg acctcggcgg gccggcagat
     1141 gccgatgaag tcgcacggga ccagccgggt cccctggtgg atgagctggt agaaggcatg
     1201 ctggccgttg gtcccgggct ggccccagag gaccgggccg gtgtccaccc cgaccggcgc
     1261 cccgtcaagg gtgaccgact tgccgttgga ctccatgtcg agctgctgga ggtaggccgg
     1321 gaagcggccc aggtactggc tgtaggggag cacggcctgg gtctcggccc cgaagaagtt
     1381 gatgtaccag accccgagca gccccagcag caccggcagg ttgcactcga acggggcggt
     1441 gcggaagtgc tcgtccatgc ggtggaagcc ggccagcagc tcgtcgtagt ggtccggccc
     1501 gatggccacc atcagcgaca gcccgatcgc cgacgggtag gagtagcggc ccccgaccca
     1561 gtcccagaac ccgaacatgt tggccgggtc gatgccgaac tcagccacct tctcggcgtt
     1621 ggtggacacg gccacgaagt ggcgggacac ggcggcctcg tcgccgaggg cgtccaccag
     1681 ccaggcccgg gccgtgcggg cgttggtcag ggtctcgatg gtggtgaagg tcttggagga
     1741 gaccacgaac agggtctcgg ccgggtccag gtcaccggtc gcctcggcga tgtcggcccc
     1801 gtcgacggtg gagacgaacc ggaacctgcg tgagcggtcg gtgtagggca gcagggcctc
     1861 ataggccatg gccgggccca ggtcggagcc gccgatgccg atgttgacga cgttgcggat
     1921 cgggcggccg gtgtggccca gccagcggcc ggagcggacc tcctcggcga aggcgcgcat
     1981 ccggcccagc acctcgtgca cctcgggcac gacgtcgtgc ccgtcgaccc ggacctggcg
     2041 gccctcggga gcgcgcaggg cggtgtggag cacggcccgg tcctcggtga cgttgatgtg
     2101 ctcgcccgcg aacatcgcgt cgcgcagctg ctcgacgccc gcacgcttgg cgagtgcgac
     2161 gagcatgcgc agcgtgtcgg tggtgacgc
//
LOCUS       DASSWR010000744         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_734532,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000744 DASSWR010000000
VERSION     DASSWR010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734532"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFO47_12480"
     CDS             <1..757
                     /locus_tag="VFO47_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET8789552.1"
                     /translation="RRAAGVLAVSSGNQGQAVARAAAETGGRAVVVMPAGSNPAKVAA
                     TRAYGAEVVTAGVTGENRETFAADLAAERGLHLVHPFDDVDVMAGQGTAALELVTDVP
                     DLDLVLVPVGGGGLIAGTAAAVKGRSPATRVVGVEPAAAADAQASLRAGRRIPLPAAP
                     ATIADGVRSLCVGERPFEVIRRLVDDIVTVTDAELLDALALCWSRTKQLVEPTAALPL
                     AALRTGAARGTRVGVVLTGGNVDAAARAACLGR"
     gene            856..1329
                     /locus_tag="VFO47_12485"
     CDS             856..1329
                     /locus_tag="VFO47_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789553.1"
                     /translation="MEPSPSSGDTGRAPRWARWFVGAYLAVFVVCGLAGLEAWPLTGW
                     RLFADARQARQAGFQAVTVDRAGRETPVPFADLPAGYQGNVQVLKGFAGLPAGEQAAV
                     CQAWATAVRERGGEVAELRIYQTVTDVSARVGDRGAPPERALRFTCRPRSADAGG"
     gene            1316..2251
                     /locus_tag="VFO47_12490"
     CDS             1316..2251
                     /locus_tag="VFO47_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8789554.1"
                     /translation="MPAADALRRAGGGRGTAGVVPRRVEGWLFAPGDPRRLAALRIGL
                     GGLLAARLASGPYPELATQPPALFRPISFLRLLDRMPSPEVVAILQALALGAAVLATV
                     GLLTRVTLPLAWLAALPLVAMTSSLGKVVHNDVLLLLCLVPLLPSRAGAAWSLDARQR
                     PVAARGPAFGWPVRTAMVVVAGAYFFSGLAKLLHAGPAWVASGNVRWVLYASSDGQPE
                     PNPFALFVADRPVLAHLVAAATLAVELGFPLVLWRPRLAWLFVPAVVAMHAGIGLAMH
                     LDYSVMAATVLVVMVDWPALADRLFPRPVPIEEQP"
     gene            2248..>3268
                     /locus_tag="VFO47_12495"
     CDS             2248..>3268
                     /locus_tag="VFO47_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8789555.1"
                     /translation="MNPEADLIIVGAPVYTADPARPWADAVAVRAGRVVSVGPAAEVG
                     ALRGPATRVLRLDGGLVLPGFADAHVHTAAGGLELAQCDLHEVEPEAYAAVVAGYAAD
                     HPGVGWVLGSGWVMDAFGTEGPRAAALDAVVADRPVLLESTDGHSAWVNRRALELAGI
                     TRATPDPPRGRIERDAAGEPTGALHEAAKDLVGDLAPEPDQAEWEAAVERGQAHLHRL
                     GITAWQDAAVEPPMLAAYRAVAERGRLTGRAVAALRWMPEDGQDQLAALVERRERGTA
                     GRLRASAVKIFADGVFENRTAAMLDPYLDGDGHPTGNLGIGMLGAEELARVVTALDAE
                     GFDVHV"
BASE COUNT          312 a         1293 c         1262 g          401 t
ORIGIN      
        1 ccggcgggcg gccggggtcc tggccgtcag ctccggcaac cagggccagg cggtcgcccg
       61 ggccgcggcc gagaccggcg gccgggcggt ggtggtgatg ccggccggct ccaacccggc
      121 caaggtggcg gccacccggg cctacggcgc cgaggtggtc accgccgggg tgaccggcga
      181 gaaccgcgag accttcgccg ccgacctggc cgccgagcgc ggcctccatc tggtccatcc
      241 cttcgacgac gtcgacgtga tggccgggca gggcacggcc gccctggagc tggtcaccga
      301 cgtccccgac ctggacctgg tgctggtccc ggtgggcggg ggcgggctga tcgccgggac
      361 cgccgccgcc gtcaagggcc gctcgccggc gacccgggtc gtcggggtcg agccggcggc
      421 cgccgccgac gcccaggcgt cgctgcgggc cggccggcgc atccccctgc ccgccgcccc
      481 ggccaccatc gccgacggcg tccgcagcct gtgcgtgggg gagcggccgt tcgaggtcat
      541 ccgccgcctg gtcgacgaca tcgtcaccgt caccgacgcc gagctgctcg acgccctcgc
      601 cctgtgctgg tcgcggacca agcagctggt cgagccgacc gccgccctgc ccctggccgc
      661 cctgcgcacc ggcgccgccc gcggcacccg ggtcggggtc gtcctcaccg gcggcaacgt
      721 cgacgccgcc gcccgggccg cctgcctggg gcgatgacca gcgcccgcgg gcgcggcggc
      781 tgggagccgg ccggggggtc cgggggaccg ccggggtggt cccccggtgg atcgggacgc
      841 ggtagcgtgc tcggcatgga gccatccccg tcgtcgggcg acaccggccg ggcgccccgg
      901 tgggcgcgct ggttcgtggg cgcctacctg gcggtgttcg tggtctgcgg gctggccggt
      961 ctggaggcgt ggccgctcac cggctggcgg ctgttcgccg acgcccgcca ggcccggcag
     1021 gcggggttcc aggcggtgac ggtcgaccgg gcgggccggg agaccccggt gccgttcgcc
     1081 gacctgcccg ccggctacca gggcaacgtg caggtcctca agggcttcgc cgggctgccg
     1141 gccggcgagc aggcggccgt ctgccaggcc tgggcgacgg cggtccgcga gcgcggcggt
     1201 gaggtcgccg agctccgcat ctaccagacc gtcaccgacg tgtcggcccg cgtcggcgac
     1261 cggggcgcgc ccccggaacg ggccctccgc ttcacctgcc gccccaggag cgccgatgcc
     1321 ggcggctgac gccctgcggc gggccggggg gggccggggg accgccgggg tcgtcccccg
     1381 gagggtcgag ggctggctgt tcgcgcccgg ggacccgcgg cggctggccg ccctgcggat
     1441 cgggctgggc gggctgctgg cggcccggct ggccagcggg ccgtacccgg agctggccac
     1501 ccagccgccg gcgctgttcc ggccgatctc gttcctgcgg ctgctcgacc ggatgccgtc
     1561 gcccgaggtg gtggccatcc tccaggcgct cgccctgggc gcggccgtgc tggccacggt
     1621 cgggctgctg acccgggtca ccctgcccct ggcctggctg gccgccctgc ccctggtggc
     1681 catgacctcc agcctgggca aggtcgtcca caacgacgtg ctgctgctcc tctgcctggt
     1741 cccgctgctg ccgtcgcggg ccggggcggc ctggtcgctc gacgcccgcc agcgcccggt
     1801 ggccgcccgg gggccggcgt tcggctggcc ggtgcggacg gccatggtgg tggtcgccgg
     1861 ggcctacttc ttctccggcc tggccaagct gctccacgcc ggcccggcct gggtggccag
     1921 cggcaacgtc cgctgggtgc tgtacgcctc cagcgacggc cagcccgagc ccaacccgtt
     1981 cgccctgttc gtcgccgacc ggccggtcct ggcccacctg gtcgcggcgg ccaccctggc
     2041 cgtcgagctc ggcttcccgc tggtgctgtg gcggccccgg ctggcctggc tgttcgtgcc
     2101 cgccgtggtc gccatgcacg ccggcatcgg gctggccatg cacctggact actcggtcat
     2161 ggccgccacc gtgctggtcg tgatggtcga ctggccggcc ctggccgacc gcctcttccc
     2221 tcgtcccgtc cccatcgagg agcaaccatg aaccccgagg ccgatctcat catcgtgggg
     2281 gcgccggtct acaccgccga cccggcccga ccctgggccg acgccgtggc cgtgcgggcc
     2341 ggccgggtcg tctccgtggg ccccgcggcc gaggtgggcg ccctgcgcgg gcccgccacc
     2401 agggtgctgc ggctggacgg cggcctggtc ctgcccgggt tcgccgacgc ccacgtccac
     2461 accgccgccg gcggcctgga gctggcccag tgcgacctgc acgaggtcga accggaggcc
     2521 tacgcggcgg tggtggccgg ctacgcggcc gaccaccccg gcgtcggctg ggtcctcggc
     2581 agcggctggg tcatggacgc cttcggcacc gaaggcccgc gcgcggccgc cctggacgcg
     2641 gtcgtggccg accggccggt gctgctggag tcgaccgacg gccacagcgc ctgggtcaac
     2701 cgccgcgccc tggagctggc cgggatcacc cgggccaccc ccgacccgcc gagggggcgg
     2761 atcgagcgcg acgccgccgg cgagcccacc ggcgccctcc acgaggcggc caaggacctg
     2821 gtcggcgacc tcgcccccga gcccgaccag gccgagtggg aggccgccgt cgagcgcggc
     2881 caggcccacc tgcaccgcct ggggatcacc gcctggcagg acgccgccgt cgagccgccg
     2941 atgctggccg cctaccgggc ggtggccgag cgggggcggc tcaccggccg ggccgtggcc
     3001 gccctgcgct ggatgcccga ggacggccag gaccagctgg ccgccctggt cgagcgacgc
     3061 gagcgcggca ccgccggccg gctgcgggcc agcgcggtca agatcttcgc cgacggggtg
     3121 ttcgagaacc gcaccgccgc catgctcgac ccctacctgg acggcgacgg ccatcccacc
     3181 ggcaacctcg gcatcggcat gctcggcgcc gaggagctgg cccgggtggt caccgccctg
     3241 gacgccgagg gcttcgacgt ccacgtcc
//
LOCUS       DASSWR010000745         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628
            k141_1101323, whole genome shotgun sequence.
ACCESSION   DASSWR010000745 DASSWR010000000
VERSION     DASSWR010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101323"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFO47_12500"
     CDS             <1..390
                     /locus_tag="VFO47_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET8789556.1"
                     /translation="EMVRGIDNDLVSFLYCAPHTFHQGGDLEGIMRYAGPLLTHLHIA
                     DSFDHRGSSGLRYILNPPGTPARIHQHADIGQGEVDWDLFFGTLADLEFDGIATVCVF
                     AWEERAADSCRHNLEQVRHYLDKHRRQ"
     gene            452..1348
                     /locus_tag="VFO47_12505"
     CDS             452..1348
                     /locus_tag="VFO47_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET8789557.1"
                     /translation="MTSTRARLRLGSCPDSWGVWFPDDPSQTPWQWFLDELAEAGYRW
                     LELGPYGYLPTDPARLRDEVDRRGLRVSGQAVFGRLHDPGGWEDDLRDAHQVAELVTA
                     MGAGHVVLLPADAGPNPPELDPDRWRTLVDRTAELGRILRDEHGLAAVFHPHADSYVG
                     TQPQIERFLEETDPADVNLCLDTGHVAYYQGDNVELIRRYPERIGYLHLKQADPAVLE
                     QVRAEGLSFGEAVGRGAMCEPPRGEPDMEALLDAVDAHLDGELFAIVEQDLYPCHPDD
                     PLPIATRTCAYLRKLGVGSDAQ"
     gene            1345..2349
                     /locus_tag="VFO47_12510"
     CDS             1345..2349
                     /locus_tag="VFO47_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8789558.1"
                     /translation="MTLGIGVVGTGQMGRYHVERLAGGVPDAEVVAVSDVFVEGAKQV
                     AEQVGARAYSDGHELIADDRVQAVLIASPGFTHEEFTLACLAADKPVLCEKPLAPTID
                     ACLRVLDAEAAKPRRLIQVGFMRRYDDGFRSLKAATDAGRVGRPLLLHCRHRNADVPP
                     GFTSDMMITDSVVHDIDVTRWLLGQEIVAATVFKARPSSLAPEGLQDPQMVMLETADG
                     VLVDVESFVTCQFGYDIRYELVGESGTIALGEHAGVQVRAEGRHHGPIPADYRERFGA
                     AYQNELQEWVSGVLRGEVTGPSAWDGYATTAVAEAAVESLTKGGRIAVELVERPALYG
                     "
     gene            2342..>2599
                     /locus_tag="VFO47_12515"
     CDS             2342..>2599
                     /locus_tag="VFO47_12515"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HET8789559.1"
                     /translation="MAEAGVGLGRVRDGMAAAADGWLASLGPEQRAKACYPFPTDEER
                     TTWYYTPTERGGLPLAEMGPVSQRLAHRLVASGLSEGGYNTA"
BASE COUNT          372 a          968 c          923 g          336 t
ORIGIN      
        1 gagatggtcc gcgggatcga caacgacctg gtcagcttcc tgtactgcgc cccccacacc
       61 ttccaccagg gcggcgacct ggaagggatc atgcgctacg ccgggccgct gctcacccac
      121 ctgcacatcg ccgactcctt cgaccaccgc ggctcctcgg ggctgcgcta catcctcaac
      181 ccaccgggca ccccggcccg catccaccag cacgcggaca tcggccaggg cgaggtcgac
      241 tgggacctgt tcttcggcac cctggccgac ctggagttcg acggcatcgc caccgtctgc
      301 gtgttcgcct gggaggagcg ggccgccgac tcctgccgcc acaacctcga gcaggtcagg
      361 cactacctgg acaagcaccg caggcagtaa ggagggacgc catgagcagg gcgggcgcat
      421 acgacaggtc agcggatgga aggttgcgcc agtgaccagc acccgggccc gcctccggct
      481 agggagctgt cccgactcct ggggcgtctg gttccccgac gacccgagcc agacgccctg
      541 gcagtggttc ctggacgagc tggccgaggc cggctaccgc tggctggagc tggggcccta
      601 cggctacctt cccaccgacc cggcgcggct gcgcgacgag gtcgaccggc gcgggctgcg
      661 ggtctccggc caggccgtgt tcggccgcct ccacgacccc ggcgggtggg aggacgacct
      721 gcgcgacgcc caccaggtgg ccgaactggt cacggccatg ggcgccggcc acgtggtgct
      781 gctcccggcc gacgccggcc cgaacccgcc ggagctcgac cccgaccggt ggcggaccct
      841 ggtcgaccgg accgccgagc tgggccggat cctccgggac gagcacggcc tggccgccgt
      901 gttccacccc cacgccgaca gctacgtcgg cacccagccc cagatcgagc ggttccttga
      961 ggagaccgac ccggccgacg tcaacctgtg cctggacacc ggccacgtcg cctactacca
     1021 gggcgacaac gtcgagctga tccgccgcta ccccgagcgg atcggctacc tgcacctgaa
     1081 gcaggccgac ccggccgtgc tcgagcaggt cagggcggag gggctgtcct tcggggaggc
     1141 ggtcggacgc ggggccatgt gcgagccgcc caggggcgag cccgacatgg aggcgctgct
     1201 ggacgccgtc gacgcccacc tcgacggcga gctgttcgcg atcgtggagc aggacctgta
     1261 cccgtgccac ccggacgacc cgctgccgat cgccacccgg acctgtgcct acctgcggaa
     1321 gctgggcgtt gggagtgatg cgcaatgacc ctggggatcg gcgtcgtcgg caccggccag
     1381 atgggccggt accacgtgga gcggctggcc ggcggcgtcc ccgacgccga ggtggtggcc
     1441 gtctccgacg tcttcgtcga gggggccaag caggtggccg agcaggtcgg ggcccgcgcc
     1501 tactccgacg gccacgagct gatcgccgac gaccgggtgc aggccgtgct gatcgcctcc
     1561 cctggcttca cccacgagga gttcactctg gcctgcctgg ccgcggacaa gccggtgctg
     1621 tgcgagaagc cgctggcccc gaccatcgac gcctgcctgc gggtgcttga tgccgaggcg
     1681 gccaagccgc ggcggctgat ccaggtcggg ttcatgcgcc gctacgacga cggcttccgg
     1741 agcctgaagg cggccacgga cgccggccgg gtcggccggc cgctgctgct gcactgccgc
     1801 caccgcaacg ccgacgtccc tcccgggttc accagcgaca tgatgatcac cgactcggtg
     1861 gtccacgaca tcgacgtgac ccggtggctg ctcggccagg agatcgtcgc cgccaccgtg
     1921 ttcaaggccc gccccagctc gctcgccccc gagggcctcc aggaccccca gatggtgatg
     1981 ctggagaccg ccgacggcgt gctggtcgac gtggagagct tcgtcacctg ccagttcggc
     2041 tacgacatcc gctacgagct ggtcggcgag tcgggcacga tcgccctggg cgagcacgcc
     2101 ggggtccagg tccgcgccga gggccggcac cacggcccga tcccggccga ctaccgggag
     2161 cggttcggcg ccgcctacca gaacgagctc caggagtggg tctcgggggt gctccggggc
     2221 gaggtcaccg gccccagcgc ctgggacggc tatgccacca cggcggtggc cgaggccgcc
     2281 gtcgagtccc tcaccaaggg gggccggatc gccgtcgagc tggtcgagcg cccggcgctg
     2341 tatggctgag gccggcgtcg gcctgggccg ggtccgcgac ggcatggccg ccgccgccga
     2401 cggctggctg gcgtcgctgg ggccggagca gcgggccaag gcctgctacc cgttcccgac
     2461 cgacgaggag cggaccacct ggtactacac cccgaccgag cggggcggcc tgcccctggc
     2521 cgagatgggg cccgtctccc agcgcctcgc ccaccggctg gtggccagcg ggctgagcga
     2581 gggcggctac aacaccgcc
//
LOCUS       DASSWR010000746         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_112805,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000746 DASSWR010000000
VERSION     DASSWR010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112805"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VFO47_12520"
     CDS             complement(<1..85)
                     /locus_tag="VFO47_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET8789560.1"
                     /translation="MAEVEIGIGKSGRRAYGFDDIAIVPSRR"
     gene            276..779
                     /locus_tag="VFO47_12525"
     CDS             276..779
                     /locus_tag="VFO47_12525"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HET8789561.1"
                     /translation="MPDPLDPFEPQDDSPPEPIDEEERAALLDDLADLAEFRSALEQR
                     GFLGVVISCPDCEEDHFFGWSLLRENLEHILQHGEPRVHEPAFEPAVDHYVTWDYAKG
                     FVDGLLEGEHEQGPLRDGWTSPAEAARRLRRALTTRGLSEDEVAAVLSEGGLPPGDAS
                     AGPDGGR"
     gene            complement(721..1560)
                     /locus_tag="VFO47_12530"
     CDS             complement(721..1560)
                     /locus_tag="VFO47_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET8789562.1"
                     /translation="MTTTSTATDRDTRLATGRAAFFDVDKTLLPGSSLYLFARGLYRR
                     GFYDLRDIAGFAFGQLVFRLTGAEGRKGMEAAREQALAFIEGKRRDDLTQIGHDIVVE
                     VIGPRIYPGMRRVIDEHNAQGDRTYLVTAAPRELAQGIATYLGMDGALGTEAELVDGT
                     YTGRLLGPVLHGPAKLDAVVRLADEEGFDLRQSSAYSDSVNDRPLLEGVGRPVAVNPD
                     RYLRALATERGWPVQDFRTRRRLWLVWLPAGMAAGVTTAAAAVTGRRLARRKRRQAGG
                     RPR"
     gene            complement(1557..>2095)
                     /locus_tag="VFO47_12535"
     CDS             complement(1557..>2095)
                     /locus_tag="VFO47_12535"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET8789563.1"
                     /translation="RFAEALAMAYHRSHGVEVRIVRIFNTYGPSMRIDDGRAFCTFAV
                     QALRGEPLTVHGDGSQTRSLCYVDDLIDGIWRLLGSDLVGPVNLGNPEEVTILERART
                     VARAAGVEPRVEFRPRPVDDPEVRRPDITRAVTELGWKPQVSLEEGVQRTVPWFRRAL
                     EAQGGSGGLKPRPPVDRG"
BASE COUNT          287 a          818 c          705 g          285 t
ORIGIN      
        1 tccgccggct gggcacgatc gcgatgtcgt cgaacccgta ggcccggcgg ccgctcttgc
       61 cgatgccgat ctcgacctca gccaccttgc gttcctccca gcaggagatt tggggggtag
      121 atagacgaac ggacccgtcc gcaaacgcgg cgggcccggg gaaggtgcat gcccgacgct
      181 agcagcgggg ttctgtggag gtcaaggaca gcccggctgg catccgcgct accatgccag
      241 ggagagcttc gcatcaacct gacttcggag tgcccgtgcc cgaccccctc gacccgttcg
      301 agccgcaaga cgactcgccc ccagagccca tcgacgagga agagcgcgcc gcgctcctgg
      361 acgacctggc cgacctggcc gagttccgct cggccctgga gcagcgcggc ttcctcgggg
      421 tggtcatctc ctgccccgac tgcgaggagg accacttctt cggctggagc ctgctgcgcg
      481 agaacctgga gcacatcctc cagcacggcg agccccgggt ccacgagccg gccttcgagc
      541 ccgcggtcga ccactacgtg acctgggact acgccaaggg cttcgtcgac gggctgctcg
      601 agggggagca cgagcagggc cccctgcggg acggctggac ctcgccggcc gaggcggccc
      661 gccgcctgcg ccgcgccctc accacccgcg gcctgtccga ggacgaggtc gccgccgtcc
      721 tcagcgaggg cggcctcccg cctggcgacg cttccgccgg gccagacggc ggccggtgac
      781 cgcggccgcg gccgtggtga ccccagcggc catgccggcg ggcagccaga ccagccacag
      841 ccgccgccgg gtgcggaagt cctgcaccgg ccagccccgc tcggtggcca gcgcgcgcag
      901 gtagcggtcg gggttgacgg ccaccggccg gccgaccccc tccagcagcg gccggtcgtt
      961 caccgagtcc gagtaggcgc tgctctggcg caggtcgaac ccctcctcgt cggccaggcg
     1021 gaccaccgcg tcgagcttgg ccggcccgtg cagcaccggc ccgagcagcc ggcccgtgta
     1081 ggtgccgtcg accagctcgg cctcggtccc cagggccccg tccatgccca ggtaggtggc
     1141 gatcccctgg gccagctccc gtggggcggc ggtgaccagg taggtccggt cgccctgggc
     1201 gttgtgctcg tcgatgaccc gccgcatccc cgggtagatc ctgggcccga tcacctcgac
     1261 cacgatgtcg tggccgatct gggtcaggtc gtcgcgccgc ttcccctcga tgaaggcgag
     1321 cgcctgctcg cgggccgcct ccatcccctt cctgccctcg gccccggtga gccggaacac
     1381 cagctgcccg aaggcgaacc cggcgatgtc ccgcaggtcg tagaacccgc gccggtacag
     1441 cccgcgggcg aacaggtaca ggctggaccc gggcagcagg gtcttgtcga cgtcgaagaa
     1501 cgccgcccgg cccgtggcca gccgggtgtc gcgatcggtg gccgtgctgg tcgtcgtcat
     1561 ccccgatcaa ccgggggcct aggcttgagg cccccggacc ccccttgcgc ctcgagcgcc
     1621 cgccggaacc agggcacggt ccgctggacc ccctcctcca gcgacacctg cggcttccac
     1681 cccagctcgg tcaccgcccg ggtgatgtcg ggtcgccgca cctcggggtc gtccaccggc
     1741 cgcggccgga actcgaccct cggctccacc ccggccgccc gcgccaccgt ccgggcccgc
     1801 tccaggatcg tcacctcctc cgggttcccc aggttcaccg gccccaccag gtcgctcccg
     1861 agcagccgcc agatcccgtc gatcaggtcg tccacgtagc acagcgagcg ggtctggctc
     1921 ccgtccccgt gcacggtgag cggctccccc cgcaacgcct gcacggcaaa ggtgcagaag
     1981 gcacgcccat cgtcaattct catcgaaggt ccgtacgtgt tgaagatgcg cacgatccgc
     2041 acctcgaccc cgtggctgcg gtggtaggcc atggccaggg cctcggcgaa gcgct
//
LOCUS       DASSWR010000747         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U628 k141_113002,
            whole genome shotgun sequence.
ACCESSION   DASSWR010000747 DASSWR010000000
VERSION     DASSWR010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968652
            Sequence Read Archive: SRR25158396
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:00:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,500
            Genes (coding)                    :: 2,455
            CDSs (with protein)               :: 2,455
            Genes (RNA)                       :: 9
            tRNAs                             :: 9
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 13 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 4 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113002"
                     /isolate="SMAG_U628"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.46194444 N 113.4388889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1540
                     /locus_tag="VFO47_12540"
     CDS             32..1540
                     /locus_tag="VFO47_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8789564.1"
                     /translation="MRAPRLVAVLAILTLLAAACGGGGGGSQSGAASSAITIAIGAEP
                     TTLDPQVRDDGNERAVNDNIYETLMARDAAGELQPGLAASAPTRKGDEAWEFKLRPNV
                     KFSNGEAFNADAVVASVTRVIDPKLNSEQASFFSTISGATKVDDLTVTIETKGPDPLL
                     PSRMYWMKMVPAQYSSEDAFADKPAGTGPYQLKEWGRGTQIVLEANPNYWGAKPQITQ
                     VTYKFVPEGGARMSGLLAGEYDLITNLLPEDVERAPKSAHLQGLEHPMIILNARGSIT
                     QDERVRQALNYAVDKQALASQLFGGLAKVDTCQILSPQMFGYNPDLQPYAYDPAKAKS
                     LLQEAGAVGKQIELVGETGRWLKDAETIQAVAGFWQDAGLKVKVSNYDFDTYLKHLFQ
                     QPNRPDAIFVSSSNELLDADRQYSNYYAEGGSGASNSDPELKRLITEARTNIDEGSRL
                     QQYQQAGKLACDKAYFTFLLNIEDIYGMSERLNWQPRVDAKLLVSGMSTAQS"
     gene            1582..>2043
                     /locus_tag="VFO47_12545"
     CDS             1582..>2043
                     /locus_tag="VFO47_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8789565.1"
                     /translation="MVRRIAQGLLVVVGVTIMVFIFTRMVGDPAKTMLPLSASPEERA
                     AFRHELGLDRPITEQFGDYIGDLASGDFGTSVWQRRPAIDAVLDALPNTFQLVLLAIA
                     IAVVLGVPLGVLAALRPGGALDRTAVVLSLAGLSIPQFWLGLLLIIAFSVTL"
BASE COUNT          355 a          735 c          662 g          291 t
ORIGIN      
        1 tccttcagga attggccgaa aggagtgggg catgagagct cctcgcttgg ttgccgttct
       61 cgccatcctg acgctgctcg ccgcggcctg cggcggcggt ggtggcggtt cccagtcagg
      121 tgccgccagc tcggcgatca ccatcgccat cggcgccgag ccgaccacgc tcgatcccca
      181 ggtcagagac gacggcaacg agcgcgcggt caacgacaac atctacgaga ccctgatggc
      241 cagggacgcc gccggcgaac tgcagccggg gctggcggcc tccgcgccga ccaggaaggg
      301 cgacgaggcc tgggagttca agctccggcc caacgtcaag ttcagcaacg gcgaggcctt
      361 caacgccgac gcggtggtgg ccagcgtcac ccgggtgatc gacccgaagc tcaactccga
      421 gcaggccagc ttcttcagca ccatcagcgg ggcgaccaag gtcgacgacc tcaccgtcac
      481 catcgagacc aagggtcccg acccgctgct gccgtcgcgc atgtactgga tgaagatggt
      541 cccggcccag tactcctccg aggacgcctt cgccgacaag ccggccggca ccggaccgta
      601 ccagctcaag gaatggggcc ggggcaccca gatcgtgctc gaggccaacc ccaactactg
      661 gggcgccaag ccgcagatca cccaggtgac ctacaagttc gtccccgagg gcggcgcccg
      721 catgtccggg ctgctggccg gcgagtacga cctgatcacc aacctgctcc ccgaggacgt
      781 cgagcgggcg ccgaagtcgg cccatctcca gggcctcgag cacccgatga tcatcctcaa
      841 cgcccgcggc agcatcaccc aggacgagcg ggtccggcag gccctgaact acgcggtcga
      901 caagcaggcc ctggccagcc agctgttcgg cggcctggcc aaggtcgaca cctgccagat
      961 cctcagcccg cagatgttcg gctacaaccc cgacctgcag ccctacgcct acgacccggc
     1021 caaggccaag tccctgctgc aggaggccgg cgccgtcggc aagcagatcg agctggtcgg
     1081 ggagaccggg cgctggctca aggacgccga gacgatccag gcggtggcgg gcttctggca
     1141 ggacgccggg ttgaaggtca aggtgtccaa ctacgacttc gacacctacc tgaagcacct
     1201 gttccagcag cccaaccgcc cggacgccat cttcgtgtcc agctccaacg agctgctgga
     1261 cgccgaccgc cagtactcca actactacgc cgagggcggc agcggcgcct ccaacagcga
     1321 cccggagctg aagcgcctga tcaccgaggc ccgcaccaac atcgacgagg gctcccgcct
     1381 gcagcagtac cagcaggccg gcaagctcgc ctgcgacaag gcctacttca ccttcctgct
     1441 caacatcgag gacatctacg ggatgagcga gcggctgaac tggcagcctc gcgtggacgc
     1501 caagctgctc gtcagcggca tgtcgacggc acagagctag cccgccgggc gggcggcaac
     1561 ggaggcagca tgggtcgctt cgtggtcaga aggatcgcgc agggcctgtt ggtggtcgtc
     1621 ggggtcacca tcatggtgtt catcttcacg cgcatggtgg gcgacccggc caagacgatg
     1681 ctcccgctga gcgcgtcccc cgaggagcgg gctgccttcc ggcacgagct gggcctcgac
     1741 cggcccatca ccgagcagtt cggggactac atcggcgacc tggccagcgg ggacttcggc
     1801 acctcggtct ggcagcggcg gccggccatc gacgccgtcc tggacgcgct gcccaacacc
     1861 ttccagctcg tgctgctggc catcgccatc gcggtcgtgc tcggcgtccc gctgggcgtg
     1921 ctggccgcgc tccggcccgg gggagcgctg gaccggacgg ccgtcgtgct cagcctggcg
     1981 ggactgtcga tcccgcagtt ctggctcggc ctgctgctca tcatcgcctt ctcggtcacg
     2041 ctc
//
